LOCUS       DAJTYL010000001        94413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYL010000001 DAJTYL010000000
VERSION     DAJTYL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.001"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..822)
                     /gene="icd"
                     /locus_tag="OO572_000001"
     CDS             complement(<1..822)
                     /gene="icd"
                     /locus_tag="OO572_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6659146.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKE"
     gene            994..1647
                     /gene="rluE"
                     /locus_tag="OO572_000002"
     CDS             994..1647
                     /gene="rluE"
                     /locus_tag="OO572_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6659147.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1657..2118
                     /gene="nudJ"
                     /locus_tag="OO572_000003"
     CDS             1657..2118
                     /gene="nudJ"
                     /locus_tag="OO572_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6659148.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2172..3278
                     /gene="mnmA"
                     /locus_tag="OO572_000004"
     CDS             2172..3278
                     /gene="mnmA"
                     /locus_tag="OO572_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6659149.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3314..3955
                     /gene="hflD"
                     /locus_tag="OO572_000005"
     CDS             3314..3955
                     /gene="hflD"
                     /locus_tag="OO572_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6659150.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            3959..5329
                     /gene="purB"
                     /locus_tag="OO572_000006"
     CDS             3959..5329
                     /gene="purB"
                     /locus_tag="OO572_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6659151.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5498..6169
                     /gene="phoP"
                     /locus_tag="OO572_000007"
     CDS             5498..6169
                     /gene="phoP"
                     /locus_tag="OO572_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6659152.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6169..7629
                     /gene="phoQ"
                     /locus_tag="OO572_000008"
     CDS             6169..7629
                     /gene="phoQ"
                     /locus_tag="OO572_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6659153.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8426..8707)
                     /locus_tag="OO572_000009"
     CDS             complement(8426..8707)
                     /locus_tag="OO572_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659154.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(8721..10382)
                     /locus_tag="OO572_000010"
     CDS             complement(8721..10382)
                     /locus_tag="OO572_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6659155.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10366..10692)
                     /locus_tag="OO572_000011"
     CDS             complement(10366..10692)
                     /locus_tag="OO572_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6659156.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(10748..10930)
                     /locus_tag="OO572_000012"
     CDS             complement(10748..10930)
                     /locus_tag="OO572_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659157.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(10914..11354)
                     /locus_tag="OO572_000013"
     CDS             complement(10914..11354)
                     /locus_tag="OO572_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6659158.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11354..11650)
                     /locus_tag="OO572_000014"
     CDS             complement(11354..11650)
                     /locus_tag="OO572_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6659159.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(11647..11985)
                     /locus_tag="OO572_000015"
     CDS             complement(11647..11985)
                     /locus_tag="OO572_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6659160.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(11982..13193)
                     /locus_tag="OO572_000016"
     CDS             complement(11982..13193)
                     /locus_tag="OO572_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6659161.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13195..13767)
                     /locus_tag="OO572_000017"
     CDS             complement(13195..13767)
                     /locus_tag="OO572_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6659162.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(13807..14964)
                     /locus_tag="OO572_000018"
                     /pseudo
     CDS             complement(13807..14964)
                     /locus_tag="OO572_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15265..15489)
                     /locus_tag="OO572_000019"
     CDS             complement(15265..15489)
                     /locus_tag="OO572_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659163.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(15631..15792)
                     /locus_tag="OO572_000020"
     CDS             complement(15631..15792)
                     /locus_tag="OO572_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6659164.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15802..16107)
                     /locus_tag="OO572_000021"
     CDS             complement(15802..16107)
                     /locus_tag="OO572_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659165.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16104..16382)
                     /locus_tag="OO572_000022"
     CDS             complement(16104..16382)
                     /locus_tag="OO572_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659166.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16671..18419)
                     /locus_tag="OO572_000023"
     CDS             complement(16671..18419)
                     /locus_tag="OO572_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6659167.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18416..18715)
                     /locus_tag="OO572_000024"
     CDS             complement(18416..18715)
                     /locus_tag="OO572_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6659168.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18721..18954)
                     /locus_tag="OO572_000025"
     CDS             complement(18721..18954)
                     /locus_tag="OO572_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659169.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(18956..19176)
                     /locus_tag="OO572_000026"
                     /pseudo
     CDS             complement(18956..19176)
                     /locus_tag="OO572_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19169..19390)
                     /locus_tag="OO572_000027"
     CDS             complement(19169..19390)
                     /locus_tag="OO572_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659170.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19380..19580)
                     /locus_tag="OO572_000028"
     CDS             complement(19380..19580)
                     /locus_tag="OO572_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6659171.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19710..19889
                     /locus_tag="OO572_000029"
     CDS             19710..19889
                     /locus_tag="OO572_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659172.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20014..20343
                     /locus_tag="OO572_000030"
     CDS             20014..20343
                     /locus_tag="OO572_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659173.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20393..20581)
                     /locus_tag="OO572_000031"
     CDS             complement(20393..20581)
                     /locus_tag="OO572_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6659174.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(20956..22185)
                     /locus_tag="OO572_000032"
     CDS             complement(20956..22185)
                     /locus_tag="OO572_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6659175.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22434..23555
                     /gene="roxA"
                     /locus_tag="OO572_000033"
                     /gene_synonym="ycfD"
     CDS             22434..23555
                     /gene="roxA"
                     /locus_tag="OO572_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6659176.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23604..24830)
                     /gene="pepT"
                     /locus_tag="OO572_000034"
     CDS             complement(23604..24830)
                     /gene="pepT"
                     /locus_tag="OO572_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6659177.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25080..26216
                     /gene="potA"
                     /locus_tag="OO572_000035"
     CDS             25080..26216
                     /gene="potA"
                     /locus_tag="OO572_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6659178.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26200..27057
                     /gene="potB"
                     /locus_tag="OO572_000036"
     CDS             26200..27057
                     /gene="potB"
                     /locus_tag="OO572_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6659179.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27054..27848
                     /gene="potC"
                     /locus_tag="OO572_000037"
     CDS             27054..27848
                     /gene="potC"
                     /locus_tag="OO572_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6659180.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27845..28891
                     /gene="potD"
                     /locus_tag="OO572_000038"
     CDS             27845..28891
                     /gene="potD"
                     /locus_tag="OO572_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6659181.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29047..29868)
                     /gene="cobB"
                     /locus_tag="OO572_000039"
     CDS             complement(29047..29868)
                     /gene="cobB"
                     /locus_tag="OO572_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6659182.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(29884..30795)
                     /gene="nagK"
                     /locus_tag="OO572_000040"
     CDS             complement(29884..30795)
                     /gene="nagK"
                     /locus_tag="OO572_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6659183.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30824..32068)
                     /gene="lolE"
                     /locus_tag="OO572_000041"
     CDS             complement(30824..32068)
                     /gene="lolE"
                     /locus_tag="OO572_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6659184.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32068..32769)
                     /gene="lolD"
                     /locus_tag="OO572_000042"
     CDS             complement(32068..32769)
                     /gene="lolD"
                     /locus_tag="OO572_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6659185.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32762..33961)
                     /gene="lolC"
                     /locus_tag="OO572_000043"
     CDS             complement(32762..33961)
                     /gene="lolC"
                     /locus_tag="OO572_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6659186.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34223..35296
                     /locus_tag="OO572_000044"
     CDS             34223..35296
                     /locus_tag="OO572_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6659187.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35424..38870
                     /gene="mfd"
                     /locus_tag="OO572_000045"
     CDS             35424..38870
                     /gene="mfd"
                     /locus_tag="OO572_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6659188.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39013..39975
                     /gene="ldtC"
                     /locus_tag="OO572_000046"
     CDS             39013..39975
                     /gene="ldtC"
                     /locus_tag="OO572_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6659189.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40058..40270)
                     /locus_tag="OO572_000047"
     CDS             complement(40058..40270)
                     /locus_tag="OO572_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6659190.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40555..41187
                     /gene="comR"
                     /locus_tag="OO572_000048"
     CDS             40555..41187
                     /gene="comR"
                     /locus_tag="OO572_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6659191.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41249..41788)
                     /locus_tag="OO572_000049"
     CDS             complement(41249..41788)
                     /locus_tag="OO572_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6659192.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42015..43319)
                     /gene="ndh"
                     /locus_tag="OO572_000050"
     CDS             complement(42015..43319)
                     /gene="ndh"
                     /locus_tag="OO572_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6659193.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43727..44269)
                     /locus_tag="OO572_000051"
     CDS             complement(43727..44269)
                     /locus_tag="OO572_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6659194.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44292..45317)
                     /gene="nagZ"
                     /locus_tag="OO572_000052"
     CDS             complement(44292..45317)
                     /gene="nagZ"
                     /locus_tag="OO572_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6659195.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45328..46152)
                     /gene="thiK"
                     /locus_tag="OO572_000053"
     CDS             complement(45328..46152)
                     /gene="thiK"
                     /locus_tag="OO572_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6659196.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46133..46774)
                     /gene="lpoB"
                     /locus_tag="OO572_000054"
     CDS             complement(46133..46774)
                     /gene="lpoB"
                     /locus_tag="OO572_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6659197.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46789..47166)
                     /locus_tag="OO572_000055"
     CDS             complement(46789..47166)
                     /locus_tag="OO572_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6659198.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47169..47528)
                     /gene="hinT"
                     /locus_tag="OO572_000056"
     CDS             complement(47169..47528)
                     /gene="hinT"
                     /locus_tag="OO572_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6659199.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            47861..50046
                     /gene="fhuE"
                     /locus_tag="OO572_000057"
                     /pseudo
     CDS             47861..50046
                     /gene="fhuE"
                     /locus_tag="OO572_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50106..51539)
                     /gene="ptsG"
                     /locus_tag="OO572_000058"
     CDS             complement(50106..51539)
                     /gene="ptsG"
                     /locus_tag="OO572_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6659200.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51834..52631)
                     /locus_tag="OO572_000059"
     CDS             complement(51834..52631)
                     /locus_tag="OO572_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6659201.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52642..53646)
                     /gene="holB"
                     /locus_tag="OO572_000060"
     CDS             complement(52642..53646)
                     /gene="holB"
                     /locus_tag="OO572_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6659202.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53643..54284)
                     /locus_tag="OO572_000061"
     CDS             complement(53643..54284)
                     /locus_tag="OO572_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6659203.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54274..55296)
                     /gene="yceG"
                     /locus_tag="OO572_000062"
     CDS             complement(54274..55296)
                     /gene="yceG"
                     /locus_tag="OO572_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6659204.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55299..56108)
                     /gene="pabC"
                     /locus_tag="OO572_000063"
     CDS             complement(55299..56108)
                     /gene="pabC"
                     /locus_tag="OO572_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6659205.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56228..57469)
                     /gene="fabF"
                     /locus_tag="OO572_000064"
     CDS             complement(56228..57469)
                     /gene="fabF"
                     /locus_tag="OO572_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6659206.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57556..57792)
                     /gene="acpP"
                     /locus_tag="OO572_000065"
     CDS             complement(57556..57792)
                     /gene="acpP"
                     /locus_tag="OO572_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6659207.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58003..58737)
                     /gene="fabG"
                     /locus_tag="OO572_000066"
     CDS             complement(58003..58737)
                     /gene="fabG"
                     /locus_tag="OO572_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6659208.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58750..59679)
                     /gene="fabD"
                     /locus_tag="OO572_000067"
     CDS             complement(58750..59679)
                     /gene="fabD"
                     /locus_tag="OO572_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6659209.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59695..60648)
                     /gene="fabH"
                     /locus_tag="OO572_000068"
     CDS             complement(59695..60648)
                     /gene="fabH"
                     /locus_tag="OO572_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6659210.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60716..61786)
                     /gene="plsX"
                     /locus_tag="OO572_000069"
     CDS             complement(60716..61786)
                     /gene="plsX"
                     /locus_tag="OO572_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6659211.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(61867..62040)
                     /gene="rpmF"
                     /locus_tag="OO572_000070"
     CDS             complement(61867..62040)
                     /gene="rpmF"
                     /locus_tag="OO572_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6659212.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62092..62613)
                     /gene="yceD"
                     /locus_tag="OO572_000071"
     CDS             complement(62092..62613)
                     /gene="yceD"
                     /locus_tag="OO572_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6659213.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62812..63396
                     /gene="yceF"
                     /locus_tag="OO572_000072"
     CDS             62812..63396
                     /gene="yceF"
                     /locus_tag="OO572_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6659214.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63508..64467)
                     /gene="rluC"
                     /locus_tag="OO572_000073"
     CDS             complement(63508..64467)
                     /gene="rluC"
                     /locus_tag="OO572_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6659215.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65040..68231
                     /gene="rne"
                     /locus_tag="OO572_000074"
     CDS             65040..68231
                     /gene="rne"
                     /locus_tag="OO572_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6659216.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68428..69370)
                     /gene="flgL"
                     /locus_tag="OO572_000075"
                     /pseudo
     CDS             complement(68428..69370)
                     /gene="flgL"
                     /locus_tag="OO572_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69382..71024)
                     /gene="flgK"
                     /locus_tag="OO572_000076"
                     /pseudo
     CDS             complement(69382..71024)
                     /gene="flgK"
                     /locus_tag="OO572_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71090..72031)
                     /gene="flgJ"
                     /locus_tag="OO572_000077"
     CDS             complement(71090..72031)
                     /gene="flgJ"
                     /locus_tag="OO572_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6659217.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72031..73128)
                     /gene="flgI"
                     /locus_tag="OO572_000078"
     CDS             complement(72031..73128)
                     /gene="flgI"
                     /locus_tag="OO572_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6659218.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73140..73838)
                     /gene="flgH"
                     /locus_tag="OO572_000079"
     CDS             complement(73140..73838)
                     /gene="flgH"
                     /locus_tag="OO572_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6659219.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(73891..74673)
                     /gene="flgG"
                     /locus_tag="OO572_000080"
     CDS             complement(73891..74673)
                     /gene="flgG"
                     /locus_tag="OO572_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6659220.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74811..75562)
                     /gene="flgF"
                     /locus_tag="OO572_000081"
                     /pseudo
     CDS             complement(74811..75562)
                     /gene="flgF"
                     /locus_tag="OO572_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75582..76721)
                     /locus_tag="OO572_000082"
     CDS             complement(75582..76721)
                     /locus_tag="OO572_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6659221.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76746..77441)
                     /gene="flgD"
                     /locus_tag="OO572_000083"
     CDS             complement(76746..77441)
                     /gene="flgD"
                     /locus_tag="OO572_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6659222.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77453..77856)
                     /gene="flgC"
                     /locus_tag="OO572_000084"
                     /pseudo
     CDS             complement(77453..77856)
                     /gene="flgC"
                     /locus_tag="OO572_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(77860..78276)
                     /gene="flgB"
                     /locus_tag="OO572_000085"
     CDS             complement(77860..78276)
                     /gene="flgB"
                     /locus_tag="OO572_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6659223.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78431..79090
                     /gene="flgA"
                     /locus_tag="OO572_000086"
     CDS             78431..79090
                     /gene="flgA"
                     /locus_tag="OO572_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6659224.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79166..79459
                     /gene="flgM"
                     /locus_tag="OO572_000087"
     CDS             79166..79459
                     /gene="flgM"
                     /locus_tag="OO572_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6659225.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79464..79880
                     /gene="flgN"
                     /locus_tag="OO572_000088"
     CDS             79464..79880
                     /gene="flgN"
                     /locus_tag="OO572_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6659226.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(79920..81455)
                     /gene="murJ"
                     /locus_tag="OO572_000089"
     CDS             complement(79920..81455)
                     /gene="murJ"
                     /locus_tag="OO572_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6659227.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81655..82488)
                     /locus_tag="OO572_000090"
     CDS             complement(81655..82488)
                     /locus_tag="OO572_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6659228.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82490..83137)
                     /locus_tag="OO572_000091"
     CDS             complement(82490..83137)
                     /locus_tag="OO572_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6659229.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83148..83732)
                     /gene="rimJ"
                     /locus_tag="OO572_000092"
     CDS             complement(83148..83732)
                     /gene="rimJ"
                     /locus_tag="OO572_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6659230.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            83968..85176
                     /gene="mdtH"
                     /locus_tag="OO572_000093"
     CDS             83968..85176
                     /gene="mdtH"
                     /locus_tag="OO572_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6659231.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85240..85887
                     /gene="grxB"
                     /locus_tag="OO572_000094"
     CDS             85240..85887
                     /gene="grxB"
                     /locus_tag="OO572_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6659232.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86021..86581
                     /locus_tag="OO572_000095"
     CDS             86021..86581
                     /locus_tag="OO572_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6659233.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86687..87733
                     /gene="pyrC"
                     /locus_tag="OO572_000096"
     CDS             86687..87733
                     /gene="pyrC"
                     /locus_tag="OO572_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6659234.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87807..88052
                     /gene="dinI"
                     /locus_tag="OO572_000097"
     CDS             87807..88052
                     /gene="dinI"
                     /locus_tag="OO572_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6659235.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88342..88596
                     /gene="bssS"
                     /locus_tag="OO572_000098"
     CDS             88342..88596
                     /gene="bssS"
                     /locus_tag="OO572_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6659236.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88711..89829
                     /gene="solA"
                     /locus_tag="OO572_000099"
     CDS             88711..89829
                     /gene="solA"
                     /locus_tag="OO572_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6659237.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            89877..89990
                     /locus_tag="OO572_000100"
     CDS             89877..89990
                     /locus_tag="OO572_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6659238.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90251..90817
                     /locus_tag="OO572_000101"
     CDS             90251..90817
                     /locus_tag="OO572_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6659239.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90821..91396
                     /locus_tag="OO572_000102"
     CDS             90821..91396
                     /locus_tag="OO572_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6659240.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91438..92490)
                     /locus_tag="OO572_000103"
     CDS             complement(91438..92490)
                     /locus_tag="OO572_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6659241.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92715..93635
                     /gene="lpxL"
                     /locus_tag="OO572_000104"
     CDS             92715..93635
                     /gene="lpxL"
                     /locus_tag="OO572_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6659242.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93807..94004
                     /locus_tag="OO572_000105"
     CDS             93807..94004
                     /locus_tag="OO572_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6659243.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94028..>94413
                     /locus_tag="OO572_000106"
     CDS             94028..>94413
                     /locus_tag="OO572_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6659244.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDP"
BASE COUNT        22673 a        25065 c        23307 g        23368 t
ORIGIN      
        1 ctctttgccg gtattcgggt ttttaacttt cagccacggg ccgccgtcga tcagttcacc
       61 gccaaattct tcacgcgcca gctggtagcc ccagtcttta aacgcgcctt cggtgaactt
      121 catgatgttg cctttgtgca ccagggtcac agagtcacga tcgttagcaa ttgcgtattc
      181 gatcgctgca cgaaccagac gtttggtgcc ttcttcagaa cacggcttaa taccgatacc
      241 gcaatgttcc gggaagcgaa ttttcttcac gcccatctct tcacgcagga atttaatcac
      301 tttctcggcg tcggcagagt cagctttcca ttcgataccc gcataaatgt cttccgagtt
      361 ttcacggaag ataaccatat cggtcagttc agggtgttta accgggcttg gagtgccctg
      421 atagtaacgt accggacgca ggcagatgta gagatccagt tcctgacgca gggcaacgtt
      481 gagagaacga ataccgccac caaccggagt ggtcagcggg cctttaatgg caacgcgata
      541 ttcacgaatc agatcaaggg tttcagcagg cagccagaca tcctgaccat aaacctgtgt
      601 ggatttttca ccggtgtaaa tttccatcca ggagatttta cgctcgcctt tataggcttt
      661 ctcgactgca gcgtcgacca ctttcagcat ggctggggtt acatctacac cgattccatc
      721 accttcaatg taagggataa tcggattttc aggaacgttg agtttgccgt tttgcagggt
      781 gatcttcttg ccttgtgccg gaacaactac tttactttcc attcacctct ccttcgagcg
      841 ctactggttt gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc gatacggatg
      901 ctttagagca attttttgtt aatgatttgt aattgacttg tcaatactac cctattgttt
      961 gccgtcatga aagatgcgcg ttattaggct ataatgcggc aattcataat ctctgaaaat
     1021 accatgcaaa aaacttcttt tagaaatcac caggttaagc gattcagctc gcaacgttct
     1081 accaggcgta aacatgaaaa ccagcccacg cgtgtgatcc tcttcaataa accctacgat
     1141 gttcttccgc agttcaccga tgaaaccgga cgcaaaacat taaaagaatt catcccggtt
     1201 cagggcgttt atgcagcagg tcgccttgac cgcgatagcg aagggttgct ggtgctgacc
     1261 aataacggcg cgttgcaggc gcgtttaacc cagccgggta aacgcaccgg aaaaatctat
     1321 tatgtgcagg tggaaggtat tcccacacaa gacgcacttg aagccttgcg caatggcgta
     1381 accttaaatg atggccctac cctgcccgca ggcgcggagc tggttgacga acccgcgtgg
     1441 ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta ttcccaccag ctggctgaag
     1501 atcaccttat atgaaggacg taatcgccag gtgcgccgca tgaccgccca tgttggtttc
     1561 cccacgctgc gactgattcg ctatgcgatg ggagattact ctttggataa tcttgccaat
     1621 ggcgaatggc gagaagtgac agattaagga tgcaaaatgt ttaaaccgca cgttaccgtt
     1681 gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg aagagacgat taatggtaaa
     1741 gcgttatgga accaacctgc cgggcatctg gaagccgatg aaaccttagt ggaagccgcc
     1801 gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc cgcaacactt tattcgtatg
     1861 catcagtgga ttgcgccaga taaaacgccg tttttgcgtt tcctctttgc cattgagctt
     1921 gagcaaatat gcccgaccca gcctcatgac agcgatatcg actgctgccg ttgggtcagc
     1981 gccgaagaaa ttttacaggc gtcaaatctt cgttcgccgc tggtggcgga aagtattcgt
     2041 tgttatcaaa gcgggcaacg ttatccgctg gagatgattg gcgattttaa ctggcctttt
     2101 acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc cgccttgaag ttcaatgtcg
     2161 tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt cggcatgtcc ggcggtgtcg
     2221 attcctccgt ttctgcctgg ctgttgcaac aacagggata tcaggtcgaa ggcctgttta
     2281 tgaagaactg ggaagaagac gacggtgagg aatattgcac agcagcagcg gatctggctg
     2341 atgcccaggc ggtctgcgac aagctcggca ttgaactgca caccgttaac tttgctgccg
     2401 agtactggga caacgtcttc gaactgttcc ttgccgaata taaagccggt cgcacgccga
     2461 atccggatat tctgtgcaac aaggagatca aatttaaagc cttcctcgaa tttgccgccg
     2521 aagatttagg tgccgattat atcgctaccg gtcattacgt acgtcgggcc gatgtcgatg
     2581 gcaagagccg cctgctgcgt ggtctggaca gcaataaaga ccagagctac ttcctttata
     2641 cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt aggcgaactg gaaaaaccgc
     2701 aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc gaagaaaaaa gactctaccg
     2761 gcatttgctt cattggcgaa cgtaaattcc gcgagttcct gggccgttat ctcccggcgc
     2821 aaccgggcaa aatcattacc gtcgatggcg atgaaattgg tgagcaccag gggctgatgt
     2881 atcacactct cggtcagcgt aaaggtctgg gtatcggtgg caccaaagaa ggtaccgaag
     2941 aaccgtggta tgtggtggac aaagacgtcg aaaacaacat tctggttgtc gctcagggcc
     3001 atgaacaccc gcggctgatg tctgtcgggt tgattgccca gcagctgcac tgggtcgatc
     3061 gcgaaccatt caccggcact atgcgttgca cggtaaaaac ccgctatcgc cagaccgaca
     3121 ttccgtgcac cgtcaaggcg ctggacgacg atcgcattaa agtgattttc gatgagccgg
     3181 ttgccgccgt gacgccgggc cagtctgccg tcttctataa cggtgaagtg tgcctcggtg
     3241 gcggtattat tgagcagcgt ctgccgctgc cggtctgatt attatcttta cttaaaacaa
     3301 ggaagcagtg aacgtggcaa agaattacta tgacatcacc ctcgccctgg ccggtatttg
     3361 tcagtcggca cgcctggtgc aacaactcgc tcaccagggg cattgtgatg ccgatgcgct
     3421 acacgtctca ctcaacagta ttattgatat gaaccccagc tcgacgctgg cggtttttgg
     3481 cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg ctcggcgtgc tcaatgccag
     3541 cagttgccag ggcttaaacg ccgaattaac ccgctacaca ctcagcttga tggtgcttga
     3601 gcgcaaactc tcctcagcga aaggcgcgct cgacactctg ggcaaccgga tcaacggcct
     3661 gcaacgccag ctcgaacact tcgatttaca gtccgaaacg ctgatgagcg cgatggctgc
     3721 tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt caggtcaccg gttcccctgc
     3781 tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca accctgctgg caggcattcg
     3841 cgccgccgtg ctctggcacc aggtcggcgg cggacgtcta caactgatgt tttctcgtaa
     3901 tcgcctgacc actcaggcaa aacaaattct tgctcattta accccggagt tgtgatctat
     3961 ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga cgctacggcg ataaagtcag
     4021 cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa ttccgtgtac aagttgaagt
     4081 acgttggctg caaaaactgg ccgcgcacgc agcgatcaag gaagttcctg cttttgctgc
     4141 cgacgcaatc ggttaccttg atgcaattgt cgccaatttc agcgaagaag atgccgcacg
     4201 catcaaaacc atcgagcgta ccactaacca cgacgttaaa gcggttgagt atttcctgaa
     4261 agaaaaagtg gcggagatcc cggaactgca cgcggtttct gaattcatcc actttgcctg
     4321 tacttcggaa gatatcaata acctctccca cgcattaatg ctgaaaaccg cgcgtgatga
     4381 agtgatcctg ccgtactggc gtcaactgat tgatggcatt aaagatctcg ccgttcagta
     4441 ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca gccacgccgt caaccatcgg
     4501 taaagagatg gcaaacgtcg cctaccgtat ggagcgccag caccgccagc ttaaccaggt
     4561 ggagatcctc ggcaaaatca acggcgcggt cggtaactat aacgcccaca tcgccgctta
     4621 cccggaagtt gactggcatc agttcagcga agagttcgtc acctcgctgg gtattcaatg
     4681 gaacccgtac actacccaga ttgaaccgca cgactacatt gccgaactgt ttgattgcgt
     4741 tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac gtctggggtt atatcgccct
     4801 taaccacttc aaacagaaaa ccattgctgg tgagattggt tcttccacca tgccgcataa
     4861 agttaacccg atcgacttcg aaaactccga agggaatctg ggcctttcca acgcggtatt
     4921 gcagcatctg gcaagcaaac tgccggtttc ccgctggcag cgtgacctga ccgactccac
     4981 cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg attgcgtatc aatccaccct
     5041 gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg ctggatgagc tggatcacaa
     5101 ctgggaagtg ctggcagaac caatccagac agttatgcgt cgctatggca tcgaaaaacc
     5161 gtacgagaag ctgaaagagc ttactcgcgg taagcgcgtt gacgccgaag gcatgaagca
     5221 gtttatcgat ggtctggcgt tgccagaaga agagaaagcc cgcctgaaag cgatgacgcc
     5281 ggctaactat attggtcgag ctatcacgat ggttgatgag ctgaaataag cctcaaatca
     5341 gtgccggatg gcgatgctgt ccggcctgct tattaagatt atccactttt tcttttttca
     5401 ctttacctcc cctccccgct ggtttattta atgtttaccc ccataaacac ataatcgcgt
     5461 tacactattt taataattaa gacagggaga aataaaaatg cgcgtactgg ttgttgaaga
     5521 caatgcgttg ttacgtcacc accttaaagt tcagattcag gatgctggtc atcaggtcga
     5581 tgacgcagaa gatgccaaag aagccgatta ttatctcaat gaacatttac cggatattgc
     5641 gattgtcgat ctcggattgc ctgacgagga cggtctgtca ctgattcgcc gctggcgtag
     5701 caacgatgtt tcactgccga ttctggtatt aaccgcccgt gaaagctggc aggacaaagt
     5761 cgaagtatta agtgccggtg ctgatgatta cgtgactaaa ccgtttcata ttgaagaggt
     5821 gatggcgcga atgcaggcat taatgcggcg taatagcggt ctggcttcac aggtcatttc
     5881 gctccccccg tttcaggttg atctctctcg ccgtgaatta tctattaatg acgaagtgat
     5941 caaactgacc gcgttcgaat acaccattat ggaaacgttg atacgcaata atggcaaagt
     6001 ggtcagcaaa gattcgttaa tgctacaact ctatccggat gcggagctgc gggaaagcca
     6061 taccattgat gtactgatgg gacgtctgcg caaaaaaatt caggcacaat atccgcaaga
     6121 agtcattacc accgttcgcg gccagggcta cctgttcgaa ttgcgctgat gaaaaaatta
     6181 ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc tgttggcaac ggcagcggta
     6241 gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga tcggttatag cgtcagtttc
     6301 gataaaacta cgtttcggct gttacgtggc gagagcaatc tgttctatac ccttgcgcag
     6361 tgggaaaaca ataagttgca tgtcgagtta cccgaaaata tcgacaagca aagccccacc
     6421 atgacgctaa tttatgatga gaacgggcag cttttatggg cgcaacgtga cgtgccctgg
     6481 ctgatgaaaa tgatccagcc tgactggctg aaatcgaatg gttttcatga aattgaagcg
     6541 gatgttaacg ataccagcct cttgctgagt ggagatcatt cgatacagca acagttgcag
     6601 gaagtgcggg aagatgatga cgacgcggag atgacccact cggtggcggt aaacgtctac
     6661 ccggcaacat cgcggatgcc aaaattaacc attgtggtgg tggataccat tccggtggag
     6721 ctaaaaagtt cctatatggt ctggagctgg tttatctatg tgctctcagc caatctgctg
     6781 ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt tacgccccat cgaagccctg
     6841 gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat tgctcaatcc agccacaacg
     6901 cgagaactga ccagtctggt acgaaacctg aaccgattgt taaaaagtga acgcgaacgt
     6961 tacgacaaat accgtacgac gctcaccgac ctgacccata gtctgaaaac gccactggcg
     7021 gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga tgagcgtcag tgatgctgag
     7081 ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa ttggctacta cctgcatcgt
     7141 gccagtatgc gcggcgggac attgctcagc cgcgagctgc atccggtcgc cccactgctg
     7201 gacaatctca cctcagcgct gaacaaagtg tatcaacgca aaggggtcaa tatctctctc
     7261 gatatttcgc cagagatcag ctttgtcggt gagcagaacg attttgtcga ggtgatgggc
     7321 aacgtgctgg ataatgcctg taaatattgc ctcgagtttg tcgaaatttc tgcaaggcaa
     7381 accgacgagc atctctatat tgtggtcgag gatgatggcc ccggtattcc attaagcaag
     7441 cgagaggtca ttttcgaccg tggtcaacgg gttgatactt tacgccctgg gcaaggtgta
     7501 gggctggcgg tagcccgcga aatcaccgag caatatgagg gtaaaatcgt cgccggagag
     7561 agcatgctgg gcggtgcgcg gatggaggtg atttttggtc gccagcattc tgcgccgaaa
     7621 gatgaataaa tatgtccttt taccactaca tcaagtcatt gattcgatca aacaattttc
     7681 attgaaaaag tgccaaaaat cgcaaattga ctacaccatt aactacaccg ttcggtgcac
     7741 tgtatgaaac aacgtggaac aaatagacac aagaaatata caggcgggta atctttccag
     7801 ggggaagcgc accaattcat gaggggcgtt aatgtcgata tggggatccc cataacgggg
     7861 ctaccggatt ttttttccgg ttaaccttta atcaggctgg tgggttttac atacccttga
     7921 tcaccgtaat gatgatccag atgtgcacct tttccaccaa tggcggaaaa gctccaaccg
     7981 taacgatgat cgctatggtt gcctcgatgg tctgcgtggt cattatgacc gttcagccag
     8041 tgaccttaat cagaattttt cgctaaaaaa tgacgttggc cacgtcatcc ggaaagcaca
     8101 aaatcacgtc gatttttaat gaacggttaa accgtctttt tgtcacggtg tgtcactggt
     8161 tgtcactctc cgtcacgatt gaggtggtgg tcattctgcc cgaccgcgca tctttgcgct
     8221 atcgggaata tcaaacagtt acctcgccga cttcctcaaa ttgaggtttc cgaaaatatc
     8281 agagcgggtt agcgattaag ctgacgagta attaaactgc gaaaatttcg tagtttctga
     8341 atatattccg gcctgctggt taacagtcct cagatgtgag ggatgtagcc agcgcaattt
     8401 tgcgctttgc tttaatctca gtgagttacc gccgtaaccg ctccggcttc ttccagtggt
     8461 acgtgatttt ctccttctcc cgatacagtt caacgcggcg attgtaggcc agcatttcca
     8521 gaacgcggat ccgtatgtcg cgcatatccg catcattaag ctggatacca tcacggcgca
     8581 tcacctcagc aacaacacgc gcataatttt cggctgtcac gctgtccggc tgcgtggtct
     8641 gttcgtcagc ctgctggctg attccagaga cgcggcggat taatcgcagt atttcggctt
     8701 ctgtcatgct gctgacctca ttacaccccg ctaaattctt ccagtttctg gcggtggctg
     8761 tcgcttatat caaaagcaaa atcctcatgc tctgcctgga atgtaccaaa cgccatcagc
     8821 gccgctacgc tcgggtctat cttgttcggt gattttttct tgttcggctt gatattggcg
     8881 ttcgcgtcac tctgcatcac gacgttactc atcgaccagg acaacaccgg atcgccacga
     8941 tgcacaatca ccctgcggtt aacaaaaact tcgaacgatt tcgccgccgg actgaatctg
     9001 aggtaggttt gcgggaacgg ctccacctca aaaccagccc cctgtaattg cgtcctgaga
     9061 tgcgtggcgt tccacgtatc aaagcccacc agcctgatat taaatttctc cgcatcctgc
     9121 atgatgtcat ctctgatccg gtcgtaatca atgcagtcac ctggcgttgt gcgtatccag
     9181 cccgctttag cccactggcg atagacagcg cggtttttat tggcggggtt ctgtagctgg
     9241 aactccggca gataatgacg ggaaatcagc ataatggttt taccgaccgg aaaggcatag
     9301 catacgctgg aaatatcgct ggttgatgat aagtccagcc ccgcgtaaca ctcctgaccg
     9361 tgtaaatctt cctccgtgaa cgttccggca cactcagccc atgcaccatt acccatccac
     9421 ggggtagtcc cctgacacca gatattaaat cgctttgtca tcatctccac ccactgcgac
     9481 ggaatacccc gcgctttctg gatggttgag gccagttttt cacgatccac ggaaacatca
     9541 atattagggt tcgcctttat ccacatcgcc ggatcatcaa cctcgttttc gtcgtccagt
     9601 tcgtaaatca gtacaaaaat tgaatcgtta acctcttcgc cgtccaggat ctggcagcaa
     9661 taatcatagt gctgtttaca ggctgaaacg acgttactgc ccgatgtggt aatggcaaat
     9721 aacagccctt cgggacgtgc gcccatcccc agctcaagcg cggaataaac gccgttatcg
     9781 gggtgtaggt ggtattcgtc cacgatggaa agactggggt ttgtcccctc gatggttgcc
     9841 gcttttgctg ccagcggctt taacaggctg ttgcttttcg ggtgtatcac cttatgcgcc
     9901 tgaatattca cccgcctgcg taacggtcgg gataaaaggc acatctgacg cgcattatca
     9961 aacacgatcc gcgcctggtc acggctcacc gctgcggtgt agatatcctg ctgaccgttc
    10021 tccataatca gaaaccagtt agccagaatc gcggcggtcg tggatttcgc atttttgcgc
    10081 ggcacttcga taaaggcgct cgtgtatttg cgccgtccgg tggccttaac cttaaagccg
    10141 aggatgcacg caaaggcgaa ctgctgccac ggctccagct caatgggtct gccacgcatc
    10201 ggtcctttta cgtgcgggca caccctggaa aaggcaataa accgctccac aacctcacga
    10261 tcgaacgtgt aaagggggct tttaaggtcc gaaaagtacc gtttaacagc ctgttttaac
    10321 cgtttacagg ccggaatttt gcccgttttt acgtcttctg cgtacttatt ccaggcggtc
    10381 aagctcgtcc tcttcttctg tttccggtgg atttttacgg cggcttatcg ggtcaaaacc
    10441 gagcaaggag gccattttta tcatcactct ttcagcgtcg gattttgcgc ttaatgcggg
    10501 gtttctgctc tcgccgccct gactgttaac aatgctgaac ccgcgcgccg caaggtctgc
    10561 aacggctttc cggtaaatgg agtagttgac gcaatacagt tccagattgc tccagtcggc
    10621 gggggtcagg tctccccgtt ccgcaagctg ccgcgatttt tcccgccact gcttcacggc
    10681 gatatcatcc aggtaggcgg ggtcgggttt gtcctgaaaa cgccccccac cccgtcatac
    10741 agcctcatca gcgattgcgg aaacattcca taacctcgcg gtcacggtcg gttaatcgct
    10801 tcgctgtgat gcgttctgcg cgtcctgacg ctttatcttt atgccctgtt tcctgtgtct
    10861 tccatgcgtc acgctgcctt ataagtccac ggataaggcg gttttgttcc cgctcattca
    10921 tcagcgtcat acatccagtt attgcggtta gcagcccgtt cttcctcttc acgaaatcca
    10981 cctgcggcac gcttgctttt tgttgccgga tcaagccatt tcgttttctg gttatgacac
    11041 gcctggcaca atggttgatg gttccattcg ggccagaaga gaacatcatc accgccatta
    11101 atcgggataa tgtgatccac caccacggcg ggcgtatata tccccttctc aaggcatcgc
    11161 acgcataacg ggtttttact cagatacatg gcgcggtatt tgtcccactg tctggaatat
    11221 ccacgcgcgc ggcggtgtcc tcgtctggca tcctctgcac gccatgccgc ccgcctgtgc
    11281 tcctcacact tgccggattt aacgcgcctg ttacagcctg gttctgtgca tcgtcttaat
    11341 ggttgccacg gcatcagtac ccccccacat cacggtaagc cgtccagagt gcgcctatcg
    11401 tcatgggtac gcgtgttttt gcgttatccg cgacaatctg gcgattttca tacaggtgag
    11461 cgataaacat catgcagcca atctttatgg ctggcgtgaa cgccagcccg tcatcaaagc
    11521 gcctgcctat gtgtatctgg cacgcctcaa gcgatgcggc aatgtatccg ctgattaact
    11581 ggtcttcatc gtcgccatcg atgcggcaat ggagtttcac ttcttccagg gtgataagtt
    11641 cttctgtcat ttttccgcgc cctcacgaca aagaatttca aggcgtgtcc tggcggcatc
    11701 cggcagcggc tgcccgatga tattcagtac acgccccgcc agcggccccg tactgacctt
    11761 tatccggctg gtggcgttga tgtccttccg gtaccgtatc cagatcctta cggtaccaat
    11821 cgccagctct gcgcccgatg aaatggcctc cttgctgctg accatgttca cgcttgccca
    11881 gagtgtgtga ccgtcctccc acgtttcgag tatttcgccg gttatatccc tggtctgttt
    11941 cagggtctga atcgttatcc tgtcacgcag tcgccctatg ttcattccgg tttttctccc
    12001 tcgctgattt ttacttcctg tttccatgcc tggctgaact catcaccacc atcacgcggt
    12061 gacagccctt cacgttcgcg ggcttcgttc ggacacataa cgtcggattt tatgccgcgc
    12121 tcataggtgg caaagcgttc gccaggtgtg gcccgtaaca ggtccgcgct gtcaaactcc
    12181 acctgatacc ggataccagg tacaggcgag gccaccagca aggcgttttt tatctgctgc
    12241 tcaaagttcg ccagccacgg gcgcatcgtc atggtgagaa atgcgcggct tgcctcgctg
    12301 aagttgctgt aggtgctgtt gctgtattcc tgcaaaaaaa tcggcgagac gttgaacatt
    12361 cgggcgatgt cttcaatggt gaagcgacgg gaggccagcc attcggcatc ctgattactc
    12421 atgcccagct gctgatagct catcccccct tcaaggatgg gcgtttttcc ggcgtttctg
    12481 gcccctttgt agcgttccag tgcggctaat gcctgtttgc ccttcacgcc gtccagccat
    12541 tcgcctgacg tgataacccc tgccgccatc atgccatcac gcatcacgct cgcgccgtgg
    12601 cgttgttggg ccagcccaag ccccagcgat tcgcggcaga tggttacagg tgagcgcccc
    12661 ataaaaccgt catccgtgga gtaacgaagg tggagaacct cccacggtaa atagttgcgg
    12721 atgtttccgg tgtaggcgtc agtgatgcag tagcgccagt tatgatcacc tgtctgctcc
    12781 acgttcaccg actgcggcgg gtaaggatgt aaagccgccg gaaaaccatc acgcccccac
    12841 tgaataaccg cataagcatt accacttaac aggcagtagc ggatcatcat tcgcttaaac
    12901 tggtagggtg tctgccacgc gttcgggcgc tcgttgagga tgtgatcgac cggatgagaa
    12961 tcaagccatt cgcgggcctc cttccccttc tcattgcgta ccaggtacag gtaacacggc
    13021 atggtagcca ccgcgtcaga gatgaccgtg acggcgttca tgacggcggg gagtgattcc
    13081 gctgtcccag atgatacgta ctcacccgcc cccgtgttcg atgtgccggc cagcgccata
    13141 aattcatcaa gcgtcatgct gcgctgctct ttttttcgtc tgaaaggcca catatcacac
    13201 ccccgctaaa tccgcccacc agcggcgatt atccgcacgc ggcatttttt cggggtgctg
    13261 ctcatacagg gaacggcggg ccagctccac gccggaatcg gggtaagccg gtaccgatgt
    13321 aacggtaatt tcgtacagtt ccgccaccag cacggtgcgc acgcatggat ctgttgtggt
    13381 atcccataca tccttacggg agcgaaagcc aaagctcatc cccgagatgt ccccacgctt
    13441 aaccagttcg ataacgtcgc gccctgtact ggtatccggt ggggtcagtt caaagcgtaa
    13501 ccctgtttcg tcctcttcca gtttcagcgt gccggaacgg gtgcgcccca gtaacatgct
    13561 gtggtcatgc tcatacaggc cgcgaacgtc attacccgcc gcaagccatt cagtaaacgc
    13621 cccccgctgg aatttttcgt aaaactcacc ccataacagt tctgaaagct tatcccagcg
    13681 aacaacgtag cccgtcagcg taccggctcc gctggtggtg atttccgatg accggatttc
    13741 catactcttc ataatttttt cacccataaa acactaaagg ggctttttag ccccttctgt
    13801 atgctgttaa tcgtcgtctt ccggcagtgc cagaatcttg atcgcgttcg aatccaccac
    13861 gccaccgcct aaatattttt gcgtgaaaat tttgatgaat cctggttctg tcaggttgtc
    13921 cggtctggtg cgaacacctg tttcgtgatc aacaatgtag taaccgcgtt tgaagtcacc
    13981 cagggcaata acgttatcag gcataaactc cagatattcg accggaaggc ccagcaacgt
    14041 atcaggatca cccgcctgta aacggtcgcg ccagatataa tcaccgttcg cgttcttcac
    14101 cttctgaagt tttgccgccg tcgtggagtt aaccactcag accgcgtttt tgcggtattt
    14161 tttacgtaat gcaaatttca ggtcgatcag cgggtccgca gatgtccacg ccagagattc
    14221 ggaaggttta attacctgca acgtaccaaa atcacgctct ttgtcgttct tctctgcacg
    14281 gggtacggat aaaaaacctt ttgctttttt gtcaccgtcg cccacaacca gatcgctttc
    14341 ttcggtttcc gtgaaggtgt cgccaatctc acccgtcagc catgaaagga tgtccacatc
    14401 ggaaaaatcc acgatttcct gtgtggtgcg cgggtacgca tagaccggat acagcttaat
    14461 gctcacctcg ttaatctgcg gggtgctggt ctgttcgcgt gccttaccct cttcaccgtg
    14521 gttaacggtc gcaccgccag cggaaacaag ctgcttaaac tcgttgctgc tgattttctt
    14581 cacggtacag atgcgacgca tggtggattc atccgccagc attcgcatga tttcggtgtt
    14641 cagttcaggg ataacggtat aaccaccatc agcgggaacg cctgtactta atgcgcgggt
    14701 ttcaccggtc agaatgtagt tacgtagttc tgcggggtca gtggtctggc tgcttttacc
    14761 tggcttgctg cgctcttcgt ctgcaatggc ttcaaggcgg gaaatgtctt tatcgaggga
    14821 ttcagcttta gcgcgtaatt cgtcaaattt tgcgccctca gcatcgttaa gactgcggtt
    14881 ttctttttca gcgttctcca gcatgtcgcg catctgattt ttaatggcgg ttttctgctg
    14941 gcgtaattcg attattctct gcataaaaaa agtcctgggg ttaagtaagg aactccagga
    15001 cgcggcaaac actcaaccgt ttttcataag gaaatcagta atcgcaccga tcgttttccc
    15061 gcctggtaat gaatattggc gagcacatta acaggcggga aagtggcccc agcgtcctgg
    15121 caccacgggc gagaataatc atgattcagt tcgggtaaaa tatgccgatc cgctcagtga
    15181 acaacgtgga acaaccacga acaaataatt tacaaaaaat aacaaaaagc cggattgctc
    15241 cggctgtatg tgctgtatgg gtgctcagtc tctgatccct ttcagaaatt ctatcaatgc
    15301 atctgtctgt tcaggattta ctgccagcat ttcaccggat agcgcgcaac gaacaaaacc
    15361 atgatgatcc ttttcaatca gcgcccccgt ttccagaaat gcgcggtaat cagtgatatt
    15421 catcgtctcc atgttgtcag catggtattt atcacgctgt ttaagtatct catcaaattt
    15481 catcggcatt gcgttttcct ctgttgtgtc tgtttgtttc agatagtaac tatgccggac
    15541 cgtgacgaaa agccagtagt gctccatacc gtttcaaatg acgcaaaaaa agccggattg
    15601 ctccggctgc gtggtgtggt tctgatattc ctactctgct aatttgctca gccccatgtc
    15661 tgcgtaagtg cgatttactg catttctcag gtctgcgtag ttctccgttg gcggctccgg
    15721 tgtgcccttg ctgctctgtt ttaccagcgg gtcgcccacc agctgcccgt aagctgcaat
    15781 ttgtgctgtc atattgccac ctcagtgaag cgatccggca ttgttctttt tcatctcttc
    15841 catcattttt tcagtgagca ttacgcactg aaaccccatg tttttgatgt ctacgggttt
    15901 catgcctttg tcagtggata tcacaggcag aatatcctga tacacatcgc ggtcagggta
    15961 actaagcaca caaagctccc atgatgccac gggtgaggat ccttcgtctg tttcaaccca
    16021 ccagcctggt gtagctgcgc ttatttgcag aatcgtgatc cgtccgttgt gcttgtaaaa
    16081 tttttcacca aattttggtt cgctcatgat tccacctctc cggttttaac gttgattgtt
    16141 gttacctgtt ccgcttcggc aatctcccgc tctgtcagcg tggcaaaatt tgccgccgtc
    16201 gtggtcatga atgcgcttat cagttcggga tgtgctttcg cgtatccttc ccccgcgtgg
    16261 cggtctatcg ttctgattgc cacctttaaa gcgtgctctg tcatgtctaa cgcgcgatat
    16321 ttcggttctg ttctgtctct gcgttttttg aatgatttat taaatttccc tgaagtgtgc
    16381 atatttattt ttaccccctc gtttaaaaag ttttgagttg tgcctcccct tgtctacctt
    16441 atctacctta gtggccctca tgccagtaat ggcgctgctt tcagcggggt agagtgcttt
    16501 tatccactat ctaccccgtg tctacctccc tgtctgattc aggtaaaatc aggtagagag
    16561 ggtagatagt gggtagacag taaaaaaagg ctatctacct aacttaatgc actgaattaa
    16621 atgtattttt ctttactcag gtagacaggg tagacagcaa ttacaaaaaa ttataaaaac
    16681 gcgtcgcact cgtctgttgt tattgcgtta gtctgcgtta ctcccttaac tttccgcgta
    16741 atatattcat gtccgtaaac tttcgcggct ggcttcatag ccttgccaaa gtcatttacg
    16801 tttagcggtt tgctcctgcc tgcgtacgcc ataaacgcca gatagacgcg gtaaaggctg
    16861 tttctggtcg tgtacttcac tgaatcgcca ccgccgccca tcatcaggcc gcgtgcttcc
    16921 tccagaaaat tcaggaactg gcaaaactca ataaccggat ccgtctgttg ctttattgcc
    16981 agagcttcat caccgtcacg ctgttccagt agtaaagccc gtgctttctc agagtcggta
    17041 aagttcgcca gcaatcggcg gataatgaca gggatttcag ccgcaatctt ttccggtagc
    17101 tccctgtctt tttcggcctc actaacgata ttgtcgaaac ggaaaatcac gcgacgacgt
    17161 gccacacctc cggcccgttc ggtgaatatc atcgggttat tgttggtcgc cagcaccacc
    17221 gccctgatta cagccgtgaa acgcttttcg tatttcgggt taatttccac ggggtcaccg
    17281 cccgtgattt tcttgatgcc cgttccttcg cctgtatatt tcggctggtc agccaggacg
    17341 ataagacgac tcccgacaac ctgcgcacgt ccaccagcat catcaagcga tgtcatttca
    17401 gcgcttaccg tgttctgttt ccctgccaga aggctggcta tgtgtgtgaa tgtactttta
    17461 ccgctcccgc cgtctccggt ggcctcaata aacatctgcc agtcgtaccg gttcgccata
    17521 atcatgtaca gcgcggcaca tatacgcatc atcttgcgct ggtcttttcc ggctgcgtgc
    17581 tcaagccatt tatgaaagtt tagcgcgtta tcgcggatgt tctcccctgg tgctggtggc
    17641 gtgtactcaa tgccgttgtg cgtggtgatc cagttctccg gcgtgtgcgg ggaaaattcc
    17701 cccgttttca ggtcaagcgc accattggcg aacggcagca aatcgccgga cggctcgccc
    17761 attggttcgg caataacttt taacgcttcc acggcgttat tgattacgcg cttgctgaaa
    17821 gtggccctgt gctctgaata gatcaccacc atttcgcggc taagttccat tgtgctgacc
    17881 ggacaccata ccccgccgcg ccatacgtga acgatttcac tttcctgatg cacacaaacg
    17941 ccatcaaagc gctcggcaag cagctgcgcg cgctcactgt ctgccatttg tgccagttgc
    18001 gtatttttct gctccggcat ggtaagccct gcggcaatat tttcacgctc agcattcaga
    18061 tagcgccgcc agttttcaca cttctggacg tgcattcctt cggggtaaaa atttgcgtcc
    18121 tgaatatctg ccgccgccag cttctgacca atctttttgg tctccaccaa atccagttct
    18181 ccggcctggt acagccttac gcgttttttt ccatccggaa caattttcag tgcatcaagt
    18241 tcggcaagct gatttggccc aagccacaca ggcggcacat tatcgccgga tgcggggcca
    18301 tcctgctcct gccattgttt cgcgtgcgac caggcatcac tacccgcaaa gataattacc
    18361 tctgtgtctt tgtgttttat tccgcgtggc tgttttttta cgttcggtgc cagtttcatt
    18421 ttttacccct gaatacgtta agcatctttt ttatttcctg aatattggcg cgtgctttct
    18481 ccctgctggt tggtttactg cggggcgctg cctgtaccag cgtgaaatca cgccggaatt
    18541 gataaacagg catcacgcaa tcatattcgt aaccttcacg gcggtaggtg atgcgccgtt
    18601 ctgccacgcc tttaatcgtt accatgccgc cgtactggtc gcggtaaata tcaccgcgcg
    18661 taaatttagg gtgagtgttg ccactggcag ttaagccaga atatttaagt ttcattattt
    18721 ttattctccg gtgtgttgtt ctttatatct gtcgtgcaat agatctattt cttgcagttc
    18781 cattattaca ggctcaagaa gcgttattaa tgccgtggca attcttgatt tttgtttgtc
    18841 gcgttcattg tcgccaagtg tttcaagcca tatgcgcaat atttccagca tgttttcact
    18901 gtgagaaagt gcaagaaatg cgcggtctat tgtttcgtgg taaatatcac gcatattaat
    18961 ccccgtccgt cgtttttctt aaaataacct ctgtcacgaa atcagcataa tcggcggcga
    19021 tattcagtac atcaagcccg ttgatttata ttctctcgtg gaaagtaaga aaaaagccgc
    19081 tctaataagt tctggcattg acgaaagcgc atcagccgca tcatcaggaa cgccggaaaa
    19141 ttcctgtttc agggaattaa aacgatcatc acgcataacc ccccccattt tcacaatcgg
    19201 caacaagaat atttttagcg tcattcagtg accgcgttgc gatgtatcgg atacattcca
    19261 gggcgaactg tgtccggtct ttctgttctt ctccctgcgc aagctctgcc gtgcattcaa
    19321 tatcaataag cgcgtgcatc agcgtagtga gtgcggcggc tgctgcgttc ggtgtggttt
    19381 tattgtgcat aggtttcccc ctgacgaata cgggcggcga atacagcaac acaaccggac
    19441 gggcaacggc tacgcgcttc gcgttccgtc caagcggtta cgtggatgat ttgggattct
    19501 ccggcactca gtgccagaaa acgccacaca aaggccgttt gtgtgtgtac aaggtgtggt
    19561 atatgattta cggcaaccat aacggctcct agtttacgtt gttggttaga agccccgtta
    19621 ctgctcctac agtgcggggc tttgcttttt gtgttgattg tgtgtaatgt gtcactacac
    19681 ataaatacat tacctgaggt gtaattaacg tgtcaacaca caaaaacgaa aggcgtggta
    19741 atcctccgtt ccagttccgt ttagatcctg acttacggga aaagatggaa aaggcacaac
    19801 aacaggacgg cgatgattct cttgctgcct ggataaagcg aataatccgc aaagaattac
    19861 agcaacgcgg catagaaccc aaaggataaa aactatcagc gccgtggtgt gaggtactac
    19921 ggcgcattgc tttacaggcg cacacaatga ccaacgaaga atcaaccgaa aaaacatcac
    19981 ctgcacgaaa aagacgacgc agaaagaaag agcatgaatc agaacgattc gcgccgtgtt
    20041 cttttgccct tgagaagtac cttaaagaat actcatcaaa ggagagaact tcgcagttat
    20101 ggcaacgcac tgaaccagac tgattgcatt gcccaccagc ctgatagcgg ctatcatccc
    20161 cgtgcttatg tttgggatca cacacacaaa cggcgcagcg ggttatctgt tcagaaaggc
    20221 ggctccattt cggggccgct ttttttatgc ctgaaaaacc ccaattttgt ggttttccag
    20281 tttcaccagg gcgaacgaat ccccgcccac gttctggcgt atattcaatc ttcatggtta
    20341 tagctctgtg ttcagatgat tgatgtgtgg cggctgcgtg ccgccagcgt gattaatgaa
    20401 ctgccttgca gctatccttc caggccagaa cctcggataa agaccagcca acggaacgac
    20461 cgccaagttt acgacgtgat gggaattgtc cggccttttc caggcggtag cggcatgagc
    20521 ggctaaggcc tgttagcttt tcgcattctt tttcacgtat aaaccgatca gtgcttaaca
    20581 ctattgcccc ctttcgtttc ttaaagagtt atttcgtgtt ctattgcgtt gggatgtgtc
    20641 tgattgtgtc aggatgattc agagttggca aatgttgagg ccgtatggtt tacagaaaga
    20701 ggaataatca ggataaaatc ctttaaattc atgttaatac aaaggcataa aatattgttt
    20761 catgcctttt ttctcgctat ttaaagagtg attcgctagt gtataaaaaa tcagtaacac
    20821 attaaaaatc agctacttat aaatctgtac actttttcac ctcttgttcg tagttgttcc
    20881 gcattgttgc tcattgttgc acgttgtatc tgttcgcata tccagtatgc gcatactgaa
    20941 aaaacacgaa aaaaattatt ttcttctggc tactggtagc gtggttacgt tttcatgtgt
    21001 tcccgccagt atccctaacc gctccgtcca catatccagc gcattgcgtt tagcatccag
    21061 ataacgggaa tgattataaa ctcgctgcat tcctggcatc tggtggcctg taagctgctc
    21121 cacgacgtga ggatccacgc ctaaatcgtt cagcatcgtt gtaaaggtgc gccggatgtc
    21181 atgtagtgac cagtgagggt gatttagcct cctgtgcgct aatctgccgt actgcgacac
    21241 gcttgtttcc tgtttcactt cccccagcaa taagcccgtg tgcctgttct gctccaccag
    21301 ctgcgtgacg aacggcagta ttgcttccgg tatgggccgg aatattgcca ccttcgtttt
    21361 gctgtgttct ttcggcacgg tccagagcat ttcggtaaaa tcccactcgc tgatctccga
    21421 taacctcagt tctaccgtcc ggcatccgaa cacaatcagg aggcggatta acgcgatgta
    21481 gtagggggag aatatttttt tgtccagtgc ctgcaataat tcgcccagtt ctttggtgct
    21541 taagacacgc tcgcttatat ccggtttttt cccaacgtcc gccacactca tatcatcaag
    21601 aacgttgctg attgcatagc gccgcctccg gcagaactta agcgcctgtt tgcacgtctg
    21661 tagcaagaat ccggcagtaa caggcgttcg ctttgccacc tggtcaaaac aggccagcca
    21721 gtgccgtagc tcgcatttat ccagcggcat agcaccaatg tgctgtatta cgtgattatt
    21781 aaggcgcttt ttcagggcgg cataatccac gcggttttcc tttgcgtacg actcaagcca
    21841 gtaggtgagc gcatcgccaa ccgttaccgg ctttaacgct tcctgtacgg tgtaattaag
    21901 ctcatgacgt ggatttttcc cctctgccag ccatgcgcga cactgggcgg ctttttccct
    21961 ggctgatttc aggctcagat caggataatt tcccagctta atgcgttccg gtggtgcccc
    22021 ccttcccgtt ccggccctgt aagtgaaata ccaggttaaa aggccactgg ttgaatgcct
    22081 gacgctcagg tttccaccgt cattaagaaa ggctgttttt tgggcgggtg tgccgttgat
    22141 tttcctcagc tgtgtatcgc tcagtttgtt aagtgctctg ctcataattt tgattccggt
    22201 catcacaatt aactacacca ttaactacac cgatcgttgc acaaagggct acaacgtgaa
    22261 acaagctgga acaaggaaaa tcacaaatct gtttataatc aaaaacataa tgaacaatct
    22321 gaaacattat gaaacggcaa aaaacactaa ttgataaaat atgtccatac ttcacgcatt
    22381 acgttaagca tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat
    22441 accaactcac tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg
    22501 tgttaaaacg cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc
    22561 tggcgatgga aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg
    22621 tcagccacgg cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg
    22681 tacaggcagt aaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac
    22741 taccggactg gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg
    22801 gcccgcatct cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc
    22861 gagtgggcga aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg
    22921 atccgttcga agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc
    22981 caggattccc gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggttttc
    23041 gcgcgccaaa tacgcgggaa ttaattagcg gatttgccga ttatgtgctg caacgtgaac
    23101 tgggcggcaa ctactacagc gatccggatg ttccacctcg tgctcatcct gcggatgttc
    23161 tgccgcaaga gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac
    23221 actttaagca atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc
    23281 cgccggaacc gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc
    23341 tggtgcgcct gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg
    23401 agaagatcga ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga
    23461 ctgcggagaa ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc
    23521 tggtcaatag cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcagca
    23581 tcacatgccg catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg
    23641 atcacctgca ccgctttttc cataccttcc agaatcacaa actcatgctt accatgatag
    23701 ttgtaaccgc cagtgaacag gttcgggcaa ggtaatccca taaacgacaa ctgcgcgccg
    23761 tcggtaccgc cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg catcgcctgc
    23821 tgggcgatat cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg
    23881 tcttcaatca ccaattcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg
    23941 atctccatca ttttacgttt acgcgcttca aactgtttac ggtcgaaatc acggatgatg
    24001 tagtgcatat cggcccgttc aacggtgcct ttcatgctcg ccaggtgata gaaaccttca
    24061 tagccttctg tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc
    24121 agcgacagcg catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca
    24181 atttttatat tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca
    24241 ccgtcaacag tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct
    24301 ttgcccactt cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc
    24361 tgttgcaata ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcagcacct
    24421 aacaaggttt taccatcggt ggtaatcaac gtctgaccca gtagctgatg cagcaccggg
    24481 aacataaccg gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag
    24541 ttttcaacaa tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga
    24601 gaaataaagc caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa
    24661 gtgcccttct cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc
    24721 agatgcaata acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat
    24781 tgggtatcca gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc
    24841 tcactttttg tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact
    24901 tttacccctg caagcgggaa tatttatcag cattacctta atgaatataa agctaagcca
    24961 gtaattcaca acaaagattg gcgattcaag cggtttgccg tagaattacc gccctcatta
    25021 ggagtcacca aggtggttaa ccacaaaccc cgcatcggta agccatccgt tgcgtttaca
    25081 tgggacagag taaaaaattg aataaacaac cgagttcgct ttcaccgctg gtgcaattgg
    25141 cgggaattcg caaatgcttt gatggtaaag aggtcattcc ccagctggat ctgactatca
    25201 acaatggcga gttcctcacg ctgcttggcc cttctggctg cggtaaaaca accgttcttc
    25261 gcctgattgc aggtctggaa actgttgatt ccggacgcat catgctggat aacgaggaca
    25321 tcacccacgt tccggcggaa aaccgctatg tgaacactgt tttccaaagc tatgcacttt
    25381 tcccccacat gaccgtgttc gaaaatgtgg cctttgggtt gcgcatgcaa aaaacccccg
    25441 ctgctgaaat tacgccccgc gtgatggaag ccctgcggat ggtgcagctg gaaaccttcg
    25501 ctcaacgtaa accgcatcag ctctctggtg gtcaacagca acgcgtcgcc attgctcgag
    25561 cggtggttaa caagcctcgt ctgttgttgc tggatgagtc gctctcagcg ctggattaca
    25621 aactgcgtaa gcaaatgcag aacgagctga aagcgttaca gcgtaagctt ggcattacat
    25681 tcgttttcgt gactcacgac caggaagaag cactcaccat gtcggacagg attgtggtga
    25741 tgcgcgatgg tcgcattgag caagacggca cgccgcgtga aatctacgaa gagccgaaaa
    25801 acctgtttgt tgccgggttc attggcgaaa ttaatatgtt taacgccacg gtgatcgagc
    25861 gactcgacga gcagcgcgta cgcgccaacg ttgaaggccg cgaatgtaat atctacgtta
    25921 acttcgccgt tgaaccgggg caaaaactgc atgttctgct gcgcccggaa gacttacgtg
    25981 ttgaagagat taacgacgac aaccacgctg aagggctgat tggttacgtt cgtgagcgta
    26041 actacaaagg catgacgctg gagtcggttg ttgaactgga aaatggcaag atggtgatgg
    26101 tcagcgaatt cttcaatgaa gacgatcctg actttgacca ctctctcgac caaaaaatgg
    26161 ccattaattg ggtagaaagc tgggaggtcg tactggctga tgaagaacac aagtaagttc
    26221 cagaatgtag tgattgtcac tattgtcggt tggcttgtgt tgtttgtctt tctgcccaac
    26281 ctgatgatca ttggcaccag ctttttgacc cgcgacgacg ccagtttcgt caaaatggtc
    26341 tttacactgg ataactacac gcgtctgctc gatccgctct attttgaagt gctattgcac
    26401 tcgctgaata tggcgctgat cgccaccctc gcctgcctgg tgctgggcta tccatttgcc
    26461 tggtttctgg cgaagctgcc acacaaggtg cgtccgctgc tgctgtttct gctgattgtt
    26521 ccgttctgga ccaactcatt gattcgtatc tacgggctga aaattttcct cagcaccaaa
    26581 ggctatctca acgagttttt gctctggctg ggcgttatcg acacaccaat ccgtatcatg
    26641 ttcacgccca gtgcggtgat tatcggtctg gtttacattc tgctgccgtt tatggtgatg
    26701 ccgctgtact ccagtatcga aaaactggat aagccgttgc ttgaggcggc gcgcgatctc
    26761 ggtgccagca agttacagac ttttatccgc atcattattc cactgacgat gccggggatt
    26821 attgccggat gtctgctggt gatgctgcca gcgatgggcc tgttctatgt gtccgacctg
    26881 atgggcggtg cgaaaaacct gctgatcggt aacgtcatca aggtacagtt cctcaatatt
    26941 cgtgactggc cgtttggtgc agccaccagc attacgctga ctatcgtaat gggcctgatg
    27001 ttgctggttt actggcgcgc ttctcgtttg ctgaataaga aggtggaact cgaatgatcg
    27061 gtcgactgct tcgcggcggt tttatgaccg ctatctacgc gtacctgtat atcccaatca
    27121 ttattttgat tgtgaactcc tttaacagct cgcgctttgg catcaactgg cagggtttta
    27181 ccaccaaatg gtatagcctg ctgatgaaca acgacagcct gttacaggca gcgcagcatt
    27241 cactaacaat ggcggtgttt tcggcgacgt ttgctacgct tatcggttca ctgacggcag
    27301 ttgcgctgta ccgttatcgc tttcgcggta agccgttcgt tagcggaatg ctgtttgtgg
    27361 taatgatgtc accagatatc gtgatggcaa tttcgctgct ggtgctgttt atgctgctgg
    27421 gtattcagct tggcttctgg tcgctgctgt tctcgcatat caccttctgc ctgccatttg
    27481 tggtggtgac ggtgtattcg cgcctgaaag gttttgacgt gcggatgctg gaagcggcga
    27541 aagatctcgg tgccagcgaa tttaccattc tgcgaaaaat cattctgcca ctggcaatgc
    27601 cagcggtggc ggcgggctgg gtgttaagct ttaccctgtc gatggacgat gtggtagttt
    27661 cttcattcgt caccggacca agttatgaaa ttctgccgtt aaaaatttat tcgatggtca
    27721 aagtcggcgt atcgccggaa gttaacgcgc tggcaaccat attactggtg ctgtcgctgg
    27781 tgatggttat tgccagccag cttattgctc gtgataaaac gaaaggtaac acaggggacg
    27841 ttaaatgaaa aaatggtcac gccacctgct cgcggcgggt gctctggcac tgggcatgag
    27901 cgccgctcac gccgatgaca acaacacgct gtatttctac aactggaccg agtacgtgcc
    27961 gccaggactg cttgaacagt tcaccaaaga aaccggtatt aaggttatct attcgactta
    28021 cgagtcgaac gaaaccatgt acgccaagct gaaaacgtac aaagacggtg cctatgacct
    28081 ggtggttcct tcaacctatt acgtcgataa aatgcgtaaa gaggggatga tccagaagat
    28141 cgacaagtcg aagttaacga acttcagcaa tctcgatcca gacatgctca acaagccttt
    28201 tgacccgaat aacgactact ccattccgta tatctggggt gcgacggcga ttggtgttaa
    28261 cggtgatgcg gtggatccga aatctgtcac cagctgggcc gatctgtgga agccagagta
    28321 caaaggcagc ctgctgttga ccgacgatgc ccgtgaagtg ttccagatgg cgctgcgtaa
    28381 gctgggctac tccggtaaca ccaccgatcc gaaagagatt gaagctgcat ataacgagct
    28441 gaaaaaactg atgccaaacg tcgcagcgtt taactccgat aacccggcta acccgtacat
    28501 ggaaggcgaa gttaacctcg gcatgatctg gaacggttct gctttcgttg cacgccaggc
    28561 gggtacgcct attgacgtgg tgtggccgaa agaaggcggc atattctgga tggacagcct
    28621 ggcgatcccg gcaaatgcca aaaacaaaga aggcgcgctg aaattgatca acttcctgct
    28681 gcgcccggat gtggcaaaac aggttgctga aactatcggt tatccaacgc caaaccttgc
    28741 ggcgcgtaag ctgttaagtc cagaagtggc aaacgataag acgctctacc cggatgctga
    28801 aaccattaaa aatggcgaat ggcagaatga cgttggcgca gccagcagca tttatgaaga
    28861 gtattatcag aagctgaaag caggacgtta attcagcaag cgggcggtgg tattaccgcc
    28921 cgttttttag ttagcaagga tgtggctaat gagtcaggac agtaaagttt tatcccgctc
    28981 tttcgtcggt attgtggtcc gcttgtaggc ctgataagcg tagcgcatca ggcaatgctt
    29041 cctgctttat aatccctcca gcaacttttc aacaaattct ggcaccacct ggcttgccgg
    29101 gccgtaatat ttctcggcaa attcattacc aacctgactc ggttcaagat tcagttcaac
    29161 ggtgtgagca ccatgtagtt tggcttcgtg tacaaaccca gccgccggat agacatgccc
    29221 ggaagtacca atggcaataa agacatccgc catcgacaac gccatataaa tttcatccat
    29281 gccgagtggc atttcgccaa accaaacgac gtgcgggcgc aagggtgccg ggaactggca
    29341 acaatggcat ttatcttctg gcgtaacgtc tccggtccag tcgagaacct gaccactttg
    29401 cgaacaacga actttcagca attccccatg catatgaatg acattggtat tacctgcgcg
    29461 ttcatgcagg ttgtctatat tctgcgttac cagcaaaaag cgatcgccga gagcgtcttg
    29521 cagtttagcc agcgcaagat gcgcggcgtt aggctgaatt tctggctgct gcagctgtcg
    29581 acgacgggcg ttataaaacg cttgcaccag ttcaggatcg cgatcgaaac cttccggcgt
    29641 tgccacatct tcaacccgat gttcttccca caggccatct gcggcgcgaa aggtacgaat
    29701 acctgattcc gcagaaattc ctgcccctgt cagtacgagt actcttggtt tttccattgc
    29761 ttccggcacc actttatctc tgaaaaaaat acgctgacgc aaacgctcgc gcaggcgacg
    29821 tttattttta cgaaaacgac ttaaccgatg accccgacgc gacagcatag caacctctgt
    29881 tgtttaatcg gttagatgta ggaaggccgc accacgcatt cctcccgcat caccgtggcg
    29941 cgcgcgttca atgcgtggaa cacgagctac aggtaagaga tgacaaggca acctgtccgc
    30001 cagttgcgtt gtgattgccg ggaaattcga taagccacca ccaatgacga ccaggtcagg
    30061 gtcaacaatg gtcaggatat ttcccagaga aaccgctaat aaatccagat aacgctcaac
    30121 gtgcgccctt gcctgctcat cgccttgatc ataaagcgca ataatttcgg gagcctgcaa
    30181 cggttgatga taatagtgtt gatacagcca cgcaaaaccg cgaccagaca gataattttc
    30241 aatgcagcca tgctgaccac agccgcagcg gcgtaacggg aaatccaacc ccatcatggt
    30301 taacgcatca accggcagac gcatatggcc aaactcgccg gtaatgtagc ttttaccagt
    30361 aatcggtttg ccgttgaaaa ccagcccgcc gccaacgccg gtgccgagaa ttaaccccat
    30421 caccagtgga tattgcgtaa attcatcatc ccaggcttct gaaagggcaa aacagttggc
    30481 atcgttatca aggcgtacat cgcgatcaag acgtgcgctc aggtcggcac gcagcggttt
    30541 gccgctggca gcggggacat tagcggcata cagcttacca tcttctgttt ccggcattcc
    30601 cggaataccg atgccgacag aacctttaca gccaaaacgt tggtcagctt cagctaccag
    30661 ctcacacact gcatctaaaa atgcgtcata gctgtcacgc ggtgtcggca cccgcttttc
    30721 ccactgcaac tgccgaccgc tatcaaacac gccaagcgca atttttgttc caccaatatc
    30781 aaacccgtaa tacattgccg ctccttgttt taatgtactg cctttactgg ccgctaagga
    30841 ctcgcgcagg gtcaatatta ctggcgcgcc gcgccggata ccaacttgcc aaaagactca
    30901 gcaacaatgc tgtgaccagt acgtagaaga cgtccagcca gtgcaattcc gatggcagga
    30961 agtcaataaa ataaatatcg ctggagagga actgatgacc gatcagcttt tcaatccact
    31021 caataatcgg ggtaagttgc agtgaaacaa ctacgccgat aaccacccca cacaggctgc
    31081 cgaacagccc tgccagcaat ccataccaga caaagatggc gcgaattaaa ccatctttcg
    31141 cccccagcgt tcttaatact gcgatatcgc cactcttgtc tttcaccgcc atcactaagg
    31201 tggagacgat gttgaaacag gccacgccaa tcaccagtac catcgccaga tacataatgg
    31261 cgcggatcat ctggatatcg cgatacatat agccgtaagt accaatccaa cttttaatat
    31321 aaacatagct gttggtcact tcacccgcat cgcgtaccag cttattggcg ttgaaaacat
    31381 ccgtcatttt aagggcaata cctgacacgc tggaacccat atcaagatat tgttgggcat
    31441 ccgccagcgg gatcatggca aaactgtgat cgagttgacc actcaactgc aaaataccgg
    31501 caatgtgcaa acgcacacgt tttggctgca tcagtttatg ctcgggattc gagttgggga
    31561 tcatgatcga cacccaatcg ccctgcttca ctttcagcgc atccgccacg cctttgccga
    31621 tgataatttg ctgttcgccc gctttaaaat tgcgccacgc atccccctga acaaacgagg
    31681 gtaatgcgct cagacgctgt tcctgttgtg ggttaacgcc cttcacctgg attgcgcgca
    31741 gattcgctcc actttccacc agaccggtga aattgatata cggcgcagcg gcggcaatac
    31801 ccggcacttt ctgcacgtta tccagtgctt cctgccagtt agtccatggc tgatttaccg
    31861 cttcgatttc gccatgcggc accaccgcca gaatgcggtt attcagttcg cgttcaaagc
    31921 cgttcatcgc gcttaagccg acgatcaata ccgccacgcc gagggcaatg ccaatggtag
    31981 aaatgacaga gattagcgac accatgccgc cgcgtcgccg tccgcgacta aaacgcaggc
    32041 caatcaataa cgataaaggc atcgccatta ctccgccccc atcaggctca gttccgccgt
    32101 cagacgtcca tcacgcattt ccagttggcg gctcatacgt ttcgccagtt gcaggtcgtg
    32161 agtgaccacc aggaaagcgg tgccctgcaa gcgattcaac tccccaagca actggaagat
    32221 gctgtcggcg ttacgcgcat cgaggttacc ggtaggttca tccgccagta ccagacgcgg
    32281 attattgacc agcgcacggg caatcgctac acgctggcgt tcgccgccag aaagttcaga
    32341 tgggcggtga ttcgcacgat gctccagccc caccgctttt aacatctcca gtgcacagct
    32401 gttgatttca gcgggctttt tcttgccaat cagcagtggc atagccacgt tttccagggc
    32461 agtaaaatcc ggcagcaggt ggtgaaactg ataaataaag cccagcttct ggttgcgcag
    32521 ttctgctttc gccgctgaag acagtttgct catcggctga ccgttaaaga tcacatcgcc
    32581 ggaggttggc gtatccagcc cgcctaacag gtgcagcaag gtacttttac cggaaccaga
    32641 gctaccgaca attgccatca tttcaccttc gcccacgctg aaactgacat tgagcagcac
    32701 atcggtttgc acactgcctt cctgatagcg tttgcacagg ttgtcgcatt gcaacaggat
    32761 cttattcata acgtaaagcc tcagcgggtt gtgtggcggc agcgcgccat gaagggtaaa
    32821 gcgtagacag cagcgcgata gccatcgcca ccagcgcaat aacaatgacc tgtaaaggtt
    32881 cgatagccac cggcagcgcc gcgccatcaa gcaggacgcc gattatcggc atcagattat
    32941 ttaactggct ggcaagcagt gcgccaagcg ccgctccgag gattgcaccg ataatcccgg
    33001 cgctagcccc ttgcaccata aagaccatca tgatttgtcg cggagttaag ccttgcgttt
    33061 gcaggatcgc gacttcgccc tgcttctcca tcaccatcag ccccagcgag gtaataatgt
    33121 taaacgccgc aacagcgaca atcaggctca gcagtaagcc catcatattt ttttccatgc
    33181 gtacggcctg gaacagctcg cctttacggt cacgccagtc ctgccatttg ctgccttcag
    33241 gcagtttttg ctgacttaaa gagtcaactc tcagcggctc atccagccac aaacgccagc
    33301 cggtaatatt ggctgccgga taacgcatca gacgcgaggc atcctcaata ttcaccagca
    33361 tttcatagcc atcgacttca ctgttggcgg cgaaagtgcc aatcacattg aacaggcgct
    33421 gacttggaat acgccccatc ggcgtgaact ggctggcaga tggcaccatc acgcggattt
    33481 gatcaccgcg attgacgccc aactgtgagg caagttgttc gccgaggatg acattatatt
    33541 tccccggctc gaggtcagtt tgtttcacat tgaccagata cggcgttagt ggatcttttt
    33601 gcgccgggtc gataccgagc atcaccccga ccgccacgct gcgcgcgctt tgcagtacca
    33661 catcaccggt agtaataggt gcgacgcgat taacgccgtc cagtttgact gccgtttccg
    33721 ggagttgctg cgggttaaga gagccatgct cagaagagag aattgcctgt ggcatcaggc
    33781 caaggatgtt gttttgcagc tcgcgctcaa agccgttcat cactgacaat actgtgacca
    33841 gcgccatcac cccgagggta atgccgatgg tagaaagcca ggagacgaaa cgaccgaagc
    33901 gatccactgc acgcccacgc atgtaacgca ggccaataaa tagagcgaca ggttggtaca
    33961 tgaaatccgt ctggttgctg tagcaaagac acgagtatat aagcgaaagc aagatgggta
    34021 aacgtctgaa ctgtaagtga tcgtcagggc taatctgaaa agcccccgta aatcaatcgg
    34081 ctaaattgcc atattttatc atcaatcttg ccgcctgatc ttaatctgaa aaatcatcca
    34141 atacagacag atacccatta cacctcgggt cataaccagc aggatcaacc gcatgtaaat
    34201 taaaaaagag cgtgttgttc tgatgaaaca aaaagagcta tggattaacc agatcaaagg
    34261 gttatgtatt tgtctggtgg tgatttatca ctcggtcatt accttttatc cgcatatgac
    34321 cacttttcag catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta
    34381 ccttgccccc tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta
    34441 tatcgacagc gtaccgtggg gaaattgtct cgataaacgg atctggaaca tcttctgggt
    34501 gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc
    34561 acctgagcgc gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt
    34621 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    34681 tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact
    34741 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg
    34801 caatttgcct tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa
    34861 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg
    34921 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    34981 gctgttttat cagtacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat
    35041 tggctccaac accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt
    35101 aactctgctt gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct
    35161 gctggtttat ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    35221 actttcacag cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    35281 cagttactct cgttaaacca tcaccccagc cggggcgatt caatttgcta aaccatgtcg
    35341 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    35401 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    35461 cgggtgagca gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg
    35521 aaattgccga acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc
    35581 tgcgtttgca tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact
    35641 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    35701 ccacccttta ccagctaccg acgatgcagc gtggcatact gattgttccg gtgaatacgc
    35761 ttatgcagcg cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag
    35821 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgccatg
    35881 ttgaccaggt gatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc
    35941 cgatggggag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc
    36001 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc
    36061 ccgcgcacga atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    36121 ataccttcga agtgaagcgc gatccggaac atatttacca gcaagtgagt aaaggcacat
    36181 tacctgctgg gatcgagtac tggcagccat tgttcttcag cgaaccactg ccaccgctgt
    36241 tcagttattt ccctgccaat accttgctcg tgaatactgg cgatctggaa accagtgccg
    36301 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc
    36361 cgctgttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    36421 actggccccg agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt
    36481 taggtttcca gaaactgcca gacctggccg ttcaggcgca acaaaaagcg ccgctggatg
    36541 cgctgcgtaa gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag
    36601 gtcgccgtga agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta
    36661 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    36721 gttttgtcga taaggcgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac
    36781 gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta
    36841 accttgcgga gctgcatatt ggtcagccgg tggtccatct ggagcacggc gtcggtcgct
    36901 atgccggaat gaccacgctg gaagctggcg gcattactgg cgagtacctg atgctcacct
    36961 atgccaacga cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg
    37021 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg
    37081 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg
    37141 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttat
    37201 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac
    37261 ttagcgacat gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    37321 gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtag
    37381 cggtgttggt gcctaccacc cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt
    37441 tcgccaactg gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga
    37501 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    37561 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    37621 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga
    37681 cgcttactgc aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc
    37741 tgtcgattat cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt
    37801 atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    37861 attatctcta caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg
    37921 tgcctgaagc acggattgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg
    37981 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    38041 ccgggatcga catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc
    38101 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc acatcatcag gcatatgcat
    38161 ggctgctgac gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    38221 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    38281 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    38341 cgctgtatat ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt
    38401 cgctggaaga tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc
    38461 cagatgattt catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg
    38521 ccaaaacgga aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc
    38581 tgccagatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    38641 tggggatcag gaagctggaa ggtaatgaga aaggcggggt gatcgaattt gccgagaaga
    38701 atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc
    38761 ttgatggtcc gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg
    38821 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg
    38881 gggcgtttct gcgcccccat ctgcaacatt tacaaattct ttgcactttc ctgcactatc
    38941 cgacactgtc accatccata attcaggctt atctgtttat tacaataacc ttatatttat
    39001 tatgtatttt tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc
    39061 gccgctgccg tggcgctggc gctaccggca aaagccaaca cctggccgct gccgccagcg
    39121 agcagtcgtc tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg
    39181 gaagccatcg ccaaaaaata taacgtcggc tttctcgctc tgttacaagc taaccccggc
    39241 gttgatcctt acgtaccgcg cgcgggcagc gtgttaacga tcccgttgca aaccctactt
    39301 ccagacgcgc cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac
    39361 ccgccgggta aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac
    39421 acgctgacac cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg
    39481 ccaacggcaa acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg
    39541 ccggctggac cggataaccc aatgggccat catgcgattc gtctggcggc ctatggcggc
    39601 gtttatttgc ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc
    39661 tgtattcgtc tgcgggatga cgatatcaaa acactcttta gccaggtcac cccaggcacc
    39721 aaagtgaata tcatcaacac tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg
    39781 gttgaagtac atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt
    39841 acgctgaata gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg
    39901 caacatgtga tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc
    39961 ccacaaactc tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa
    40021 aaaatcagca gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc
    40081 catggagggt attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat
    40141 ccgctttttg cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga
    40201 tagtaccgac tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg
    40261 caaatgacat ggagcttaaa atcgccgcag cgatgaggtt tttacgtttt tcataatagt
    40321 ggccttatgc agatgaatga cgtcgcaaga aattacttaa cgatcgataa ctaaatgata
    40381 gatgtgatct ggatcacata caagatattt tttataacaa tcattaatta atttaaaaat
    40441 ctttaaattt cagtcaaata gaaagcaatt atttttatgg gatctggact ggtgaacgga
    40501 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca
    40561 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
    40621 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
    40681 gcggacctcg tcgaagcaac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
    40741 aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat
    40801 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
    40861 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
    40921 tgcaccaccc tctccccaga ttcaggagat atagccaata cgttgaaatc acgccatgcg
    40981 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
    41041 acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg
    41101 atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgccgg aacgactttg
    41161 cgtttatggc ccgaactggt gaaataagtc gcaaaaaagg cccctcagcc tgagcgaatg
    41221 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt
    41281 tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat
    41341 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
    41401 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
    41461 taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta ccgcgaccac
    41521 caccaaactg atgccctatc acgccaccag caacagcgcc gagcaccgac ccggtaatgc
    41581 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
    41641 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggcccc
    41701 gttcaaacac gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc
    41761 cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat
    41821 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatgactg
    41881 gaaatactta aggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
    41941 tatcaggcct accgctcgta ccatttgtcc agggcggata aggcgtttat accacatccg
    42001 ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc
    42061 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
    42121 agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
    42181 ttacccatca ggctaccaac ggtggagaag ttcgacagcg ataccagcga accatgatct
    42241 ttatactgat aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg
    42301 caagtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc
    42361 gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgagtggt ttgcagcgtc
    42421 ggttccacca ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag
    42481 tctggcgctt tgatcccggc tgcccatacc atcagatcag cctcaatata ttcgccatct
    42541 ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg
    42601 ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa
    42661 atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa
    42721 ccgtagctgt gcagttgctt gatcgcgttg tgcaattcag cggagagttc tacacccgtc
    42781 gcgccgccgc cgacaatcgc aatgttcact ttgccatttg cgcccaggtt ggcagagtat
    42841 ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
    42901 aaaatgcagt tctctttgac gcctggcgta ttgaaatcgt tagaggtgct acccagcgcc
    42961 atgaccaggg tgtcataggc gattttacgt tccggaacca acagttcacc tttctcgtcg
    43021 cgtagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccaac
    43081 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
    43141 tcaagcgagc cagtcgccac ttcgtgcagc agtggtttcc acaggtggct gtggttacga
    43201 tcgaccagcg taattttggc ttttttctta cgtcccagct tatgccccag ctgtgttgcc
    43261 atttccagcc caccagcacc gccgccgaca atcacaatct ttttcaatgg cgtagtcaac
    43321 gtgcccccct taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat
    43381 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
    43441 ttggtcatac caaaattgat gttaatcaag ttttgttgct taaaagatag gcaactttac
    43501 cgggggagat caaaaaatag agtttattgt ttattatttg agcaaattcg gaagggtaat
    43561 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagatgcgc agcatcgcat
    43621 caggcattga tgctcgactg tcggatgcgg cgtggacgcc ttatccgact tacagaattc
    43681 ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
    43741 tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt
    43801 cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagtg
    43861 cttcatcatt acgcgacaaa ataaccagac aacgatcgcg attcttctcg cggaagttgg
    43921 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt
    43981 tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa
    44041 tccgttccgc ccagtatccg cccaggccaa cgccgcaaat tagcggacgc tcgtcaacgt
    44101 tcagttgcaa cattttgtcc acttctttaa gcagatgctg catatcatgt ttcggatgcc
    44161 gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct
    44221 cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac
    44281 ataagccagg gttagtgacc tactttctct tcctgccagc gttcatgtaa ctgattcaga
    44341 cgggtgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
    44401 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
    44461 acggcccctt tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatgcc
    44521 tgcccgcgtt cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag
    44581 aaaatcacgc cgtcaaaccc cagttcctga cgcaaaacgg ttttcagcca gtagggagaa
    44641 ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg
    44701 agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct
    44761 tgtggacgtg gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct
    44821 gggaagtgtt tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg
    44881 gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg
    44941 atatgcccga catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg
    45001 ctggccatca gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc
    45061 gcagcaaagg attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt
    45121 ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc
    45181 accagttcac gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc
    45241 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc
    45301 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
    45361 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
    45421 tcctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
    45481 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc
    45541 cacccacacc gccgccagtt ccagcgcgat atcaccatct ccagcatact cccagtcgat
    45601 gagttttaac cctgatgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
    45661 acttaagcgt aaaggtcgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca
    45721 acccacagtc cgccgcgccg gatcgctttg ctgccagtac agttccagta agggcaacag
    45781 cgttattcgc cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc
    45841 gttggtatcc ggcaaatacg tttttaccgc accgggcaga tagtcgacta ccatccagtc
    45901 acggagatat aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata
    45961 ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
    46021 ctggccctga tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac
    46081 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct
    46141 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
    46201 tttgtagggt cggagcgtta acgttgccag aggcgcagga gtacagcacg taatgagcgc
    46261 cgacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
    46321 aacctaactg ttgcttagcc atcgacagtt gctgggcgga aaccagggta aatttcccgt
    46381 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag
    46441 tacggttgtt aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa
    46501 gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg
    46561 caggcggtgc cgttcgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca
    46621 ccgagggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca
    46681 cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg
    46741 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcaccccttt acaaatatag
    46801 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga
    46861 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
    46921 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaatcgt
    46981 tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc
    47041 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
    47101 atgtgaacga cagcccacca gcagtaacaa caccagagac accagcccaa agcatccttt
    47161 tctcatcgtt acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag
    47221 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtattcat gatcagacga
    47281 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
    47341 cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag aatattcgga
    47401 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
    47461 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
    47521 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
    47581 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaatcttaa ctcacgattt
    47641 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
    47701 aatgcgctga aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc
    47761 tcatttgcat ttgcagcaaa attattcgca cactaaatat aaccgctcat caccctcctc
    47821 cggcatgagc ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa
    47881 cagggataat caatatcaag tcatcaccaa accgtcacta cttgccggtt gcatagcact
    47941 ggcactatta ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga
    48001 gggttcagcc acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc
    48061 gggtaccaaa atgcagatga ctcaacatga tattcctcag tcggtcacta ttgttagcca
    48121 gcagcggatg gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg
    48181 gatcagcaaa agtcaggcgg attccgatcg tgctctttat tattcccgcg gattccagat
    48241 cgataactat atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga
    48301 cgcactttct gatatggcac tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact
    48361 catgaccggg acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgacctg
    48421 tcgtgaattt aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt
    48481 ggcggattta caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg
    48541 ctaccagaat aacgactcat ggctggaccg ctacaatagt gaaaagacct tcttctcggg
    48601 cattgtcgat gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg
    48661 cattgatgtt aatagcccaa cctggggcgg tttaccgcgc tggaatactg atggcagcag
    48721 caacagttac gatcgcgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat
    48781 caacaaggtc tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa
    48841 tgccacccac tctgaagtcg aatttgacag caaaatgatg tatgtcgatg cctatgtaaa
    48901 caaagcggat ggtatgctgg ttgggccata cagtaattat ggacctggct ttgatcatgt
    48961 cggcggcacc ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga
    49021 cggtagttat gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa
    49081 acaaaacaat cgttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac
    49141 aactttaatg gcaatttccc acaagccgac tggtcatcac agagcctggc gcaggacgat
    49201 accacacata tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
    49261 ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag
    49321 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
    49381 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
    49441 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
    49501 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
    49561 ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc
    49621 agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt
    49681 ggcgcaacgc gctatcttgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
    49741 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc
    49801 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc
    49861 ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg
    49921 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
    49981 atggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
    50041 ttctgaataa cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt
    50101 acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta
    50161 ccgaaaatag cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
    50221 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
    50281 gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt
    50341 tcgctggtac ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga
    50401 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg
    50461 acgatcggta acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga
    50521 ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg
    50581 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg
    50641 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    50701 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    50761 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    50821 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc
    50881 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg
    50941 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    51001 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    51061 gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc
    51121 agcttaatac ggtagaaacg gttaaacatg tacgctgcaa tcgcaccgga gataatccct
    51181 ccgagtacgc cagtatccgc caagtgttta gaggcgattt cttcagcagg taaatgcagt
    51241 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc
    51301 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt
    51361 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    51421 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg tagtacggat
    51481 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    51541 attgagagtg ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga
    51601 gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat
    51661 tcagtgccaa tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca
    51721 cattttgaca aaacatatgt cacaatattt caaacgcggt tattcaccgc ccaattttcg
    51781 gcggtgaact ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg
    51841 atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc
    51901 tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga
    51961 accatcgcag gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca
    52021 gtttccacca gtaatcgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca
    52081 ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc
    52141 gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct
    52201 tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg
    52261 ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt
    52321 tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc
    52381 gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca
    52441 caagaaaata cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt
    52501 aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg
    52561 tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg
    52621 actaaaaaca tgatgtctct cttaaagatg aggaaccggt aacacaacgc ccggttgcag
    52681 gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac
    52741 agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcacgaggg
    52801 agaaagatgg tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc
    52861 agcgccatga tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc
    52921 cggagcttgt tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct
    52981 atatgccaac gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc
    53041 cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga
    53101 cattgtcact tcgcgtgaaa gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta
    53161 atgtaaccga caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag
    53221 gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc
    53281 gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg
    53341 ctcattcagc ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt
    53401 ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg
    53461 acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc
    53521 gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc
    53581 tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca
    53641 tctcatgcgt ccaactcctt cacccagtgg gtcacggtag tgcggattgc atccatcacg
    53701 gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc
    53761 agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc
    53821 tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt
    53881 aagtctgggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca
    53941 ataccacgtc ctccgccctg atacgcctga gtggaaagat cgtggcgatc gccaatcacc
    54001 caggtgccgt tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca
    54061 taaaacatca gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc
    54121 agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc
    54181 atgtcgcgga tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg
    54241 cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac
    54301 tttcagataa tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc
    54361 acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt
    54421 cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt
    54481 gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc
    54541 catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat
    54601 aaataccgag gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga
    54661 tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca
    54721 ggcgctatcg accgctttca ccattttctt gtgaactcgc ttgagtaacg cgacatcggt
    54781 ggtattggcg gtatacatca aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt
    54841 ttccagttca agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat
    54901 atacggagcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg
    54961 cagcgggaac tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat
    55021 ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag
    55081 cagccattga aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag
    55141 cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt
    55201 gctgtcggca agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac
    55261 cagcaataac aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt
    55321 ggggctaaat attcatataa cgttgctgac gaaaaggtga catcgccaca ggcacgtacg
    55381 ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcctcc
    55441 agcgaggctt gcacttcgac aagctgataa gaggattgtg ccagtaaacg gatacagaat
    55501 tgtcgcataa tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacattgccc
    55561 ttccgccaga acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc
    55621 agcgcctcat cagcgtttgt ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga
    55681 ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc
    55741 aacgtcatcc cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga
    55801 atccgcgttg ctggtccgct gttcaatgtg ctgtaccctc gcccgccact accgcgactg
    55861 atcacgactt tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca
    55921 agctgaggcc agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg
    55981 atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca
    56041 tcaccaaact gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt
    56101 aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac
    56161 aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa
    56221 tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt
    56281 tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat
    56341 cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg
    56401 ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag
    56461 atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct
    56521 gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct
    56581 gactcgcttc aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc
    56641 cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt
    56701 cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac
    56761 cggcaccatc gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt
    56821 gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg
    56881 gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa
    56941 tacgcgcagc atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc
    57001 ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg
    57061 acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt
    57121 cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt
    57181 tctcttccgt tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat
    57241 attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa
    57301 aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta
    57361 ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga
    57421 caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc
    57481 aaggagggaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    57541 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    57601 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    57661 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    57721 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    57781 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    57841 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    57901 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    57961 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    58021 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    58081 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    58141 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    58201 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    58261 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    58321 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    58381 ttttgacaga cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc
    58441 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    58501 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    58561 attcaacatc agacctttgc cgttggcacc taaataatca ctgatcgcct gagcgccatt
    58621 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    58681 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    58741 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    58801 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc
    58861 atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg
    58921 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    58981 atcaacgtta ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac
    59041 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa
    59101 cggcagcgcg cgttttgcgc cagcagcttt acaggcagcg ccagcacgct caaccgcttc
    59161 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    59221 accttctgca gcttcttcac acgctttcgc aataggcgca tcatccagac cgatgattgc
    59281 cgccatagcg cccgtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac
    59341 cagacgcacc gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc
    59401 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata
    59461 cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    59521 agctgacccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    59581 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    59641 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    59701 acgaaccagc gcagagcccc aagtgaatcc accgccaaag gcttcaagca gaaccaactg
    59761 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    59821 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt
    59881 tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    59941 agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac
    60001 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    60061 gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt
    60121 ggaaatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    60181 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    60241 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    60301 ggtgaaacct gcacaggctg ctgcaacgtc aaatgccggg caacctttaa tgtccaacat
    60361 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    60421 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    60481 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    60541 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    60601 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    60661 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    60721 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    60781 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    60841 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    60901 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    60961 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    61021 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat
    61081 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    61141 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    61201 tactgtcaag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    61261 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    61321 tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    61381 gtggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    61441 ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    61501 ctttcaccag ctccagggcc atacgcattg aactcccacg gctggcgcgg atagcttgcg
    61561 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    61621 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    61681 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    61741 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    61801 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    61861 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    61921 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    61981 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    62041 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    62101 ctttaagctg gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc
    62161 aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    62221 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    62281 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    62341 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    62401 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    62461 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    62521 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    62581 atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    62641 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    62701 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    62761 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    62821 cttattttag cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct
    62881 tttgaatgtg cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    62941 ttggtgcttc gactggcaca agaaaaatcg caatctctgg cgtcacgtta tccggatcat
    63001 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    63061 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    63121 actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt
    63181 gacgtccatt tccgtcacct aagcgaggcg gagattgata attacgtgcg taaggagcat
    63241 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt
    63301 ttagagggac gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    63361 ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg
    63421 tcgcatccgg caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc
    63481 gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    63541 cttcaaacct tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    63601 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    63661 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    63721 ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt
    63781 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    63841 gacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct
    63901 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt
    63961 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    64021 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    64081 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    64141 ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc
    64201 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    64261 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    64321 cacccgcact tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    64381 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    64441 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    64501 gccactcatt tttctatgct tatttttagt tttgcacctt attacttcac tacgtgatca
    64561 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    64621 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    64681 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    64741 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    64801 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    64861 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    64921 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    64981 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    65041 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    65101 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    65161 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    65221 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    65281 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    65341 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    65401 gtctggcggg tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc
    65461 gccgtatcga aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    65521 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc
    65581 aatgggattt aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    65641 gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt
    65701 atttacgtca ggatatcggc gaaatcctta tcgataaccc gaaagtcctc gaactggcac
    65761 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    65821 gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc
    65881 gtgaagtccg tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaacag
    65941 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta
    66001 acaccaacct cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    66061 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    66121 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    66181 gctttggcct gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc
    66241 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc
    66301 tctctattct gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    66361 ccattgttcc tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg
    66421 cgattgaaac gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa
    66481 ccccgcacta ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca
    66541 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    66601 gtaagcgtcc tgaacaacct gcactggcaa cctttgccat gccggatgta ccgccagcgc
    66661 caaccccagc tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa
    66721 caccagcagc tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc
    66781 tgttcagcgg tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga
    66841 aaccggaacg tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg
    66901 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    66961 gtaatcgtcg ccaggcacag cagcaaactg ccgagacgcg tgagagccgt cagcaggctg
    67021 aggtaacgga aaaagcacgt accaccgacg agcagcaagt gccgcgtcgt gaacgtagcc
    67081 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    67141 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    67201 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    67261 tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc
    67321 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    67381 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    67441 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    67501 caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    67561 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg
    67621 aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    67681 aacctgttgt tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac
    67741 cggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    67801 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg
    67861 cagaacacgc agaaccgatg gttgaaccgc aggaagagac ggcagatgca gatattgaag
    67921 aagttgccga aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg
    67981 cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga
    68041 tcaccgttga gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg
    68101 ttccggaggc accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag
    68161 gtgccgcagg tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac
    68221 ctgttgagta ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat
    68281 ttcatgtttg aatcaacata ttaaaattca tcaaatggca tccttgctaa ccaacaatgc
    68341 aaaataggca aaaaaaatgc ccgccccggc gatcccagag cgggcagaca aaacataccc
    68401 agtttcatga tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg
    68461 catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga
    68521 aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc
    68581 gcgatcgcta cctaaggaat ccagcgactc cagtttgttc agctgcgtgc ctaattccgc
    68641 gcgaacggta aggacattat tcagcgagtt tttcagtcca cggttggttt tatccaatgc
    68701 cgcagcggcg atttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat
    68761 ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac
    68821 cgcgttactg gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg
    68881 cgaagcatcg acctgttgct taatactttc tgttccgcca acataatcac cgttagcctc
    68941 actaaacggc gcagtctctg ttttatatgt agcgcccatt accgtcagtg gtgttcgcca
    69001 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt
    69061 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg
    69121 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca
    69181 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa
    69241 tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca
    69301 tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac
    69361 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    69421 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    69481 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    69541 attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
    69601 taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg
    69661 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    69721 catcgcgatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
    69781 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    69841 atcaaatgcc actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag
    69901 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac
    69961 ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag
    70021 aaccgcgggc ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc
    70081 ggctttgtgt tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt
    70141 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    70201 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt
    70261 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
    70321 cagtgagtaa ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct
    70381 acgccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta
    70441 ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt
    70501 tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg
    70561 tcctggtcgc gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc
    70621 ccaatcagcg cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg
    70681 gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg
    70741 attttcgaca tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt
    70801 aactggttgg taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg
    70861 ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg
    70921 gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg
    70981 gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag
    71041 ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat
    71101 tatcaatgtt catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga
    71161 tcatgttggt gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga
    71221 cctgcgcccc ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg
    71281 ttaacagccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg
    71341 cttttacttt cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc
    71401 ctttccagtt gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc
    71461 ggcggatttg ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat
    71521 gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg
    71581 ctctactgtc acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct
    71641 gcgaaagcgt ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg
    71701 ctggcgtgga ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg
    71761 caagccccag acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg
    71821 tatacagacg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc
    71881 ttttcaacat catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg
    71941 ccgccggatc ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg
    72001 cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca
    72061 tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc
    72121 attgagcgca cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc
    72181 gccgctctgg cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa
    72241 cggtgtatct ggctggctga cattggcctg acgattaact gttactgaga gatttccctg
    72301 cgctaccgcg cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga
    72361 gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc
    72421 aaggaaacgg acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc
    72481 taacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc
    72541 catgctgaaa tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct
    72601 gggcaattca cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac
    72661 ctgaacactg ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc
    72721 cagcgcatac acctgactgt caacgccctt aagcggagtc atcaacaacg tacctccacg
    72781 caagcttttg gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc
    72841 aaacggagga agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt
    72901 gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa
    72961 cggcgtctgg gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat
    73021 cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc
    73081 cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact
    73141 tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta
    73201 atgtagccat tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta
    73261 ttgctgccgc tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc
    73321 tgattaatgg caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg
    73381 tcaaccgtca ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg
    73441 ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc
    73501 aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca
    73561 gaggagcttt tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata
    73621 ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac
    73681 tggaaaatag aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc
    73741 ccctgcacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag
    73801 ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag
    73861 taaaccggtg aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg
    73921 atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt
    73981 gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc
    74041 cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta
    74101 gaggttttcg ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg
    74161 cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc
    74221 gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc
    74281 agccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga
    74341 tgagccatct ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct
    74401 gttattggtc tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac
    74461 ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg
    74521 ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt
    74581 agtactgacg tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag
    74641 gcccgttttg gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca
    74701 agcagagcag aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg
    74761 ggcgagggaa tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc
    74821 gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc
    74881 atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta
    74941 ctgccttcca gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct
    75001 cgcgtggcct gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg
    75061 ctgcccgtgg ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg
    75121 cccggattga gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc
    75181 acagcaattg gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg
    75241 ggatcaacct gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg
    75301 gccagccatc ctgctgcaac gcaacgtcca gcgggcgaga ggtgtaatcc atcttgccgg
    75361 gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc
    75421 cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat
    75481 tggccagatt actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg
    75541 cagtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc
    75601 gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg
    75661 gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg
    75721 ttggtcagag tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat
    75781 tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc
    75841 gccagtacaa tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta
    75901 ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt
    75961 gccacaatgt tgttagcgcc ggttgcgata ttattcgcca tcgcaccatc cactaatgtg
    76021 ccattagcat taaattccag tgttgtcgct gtcttcgcaa tgctgtttgg atcactgcta
    76081 tcctgggtgt agacctgcca gttattatcc ccggtcttca caaaatagac gctcatgtca
    76141 tgagcattac cctgactgtc gaaaacagtc accgaacctt ttttgttata gctatccgca
    76201 ttgctggcgc tgaatggcgt aacagtagga agcggatcac tggaattcag gttgatctgc
    76261 atcgacgccg tggtggtagt tttcgctgcc atcagggtat tcgggatcga aatattggtc
    76321 ggattcgccc cttgctgaat agtcggcggc gtaccggtta ccgggtaacc cgtcagctgt
    76381 aaaccttgcg tattcaacag gttacggttt tcatccagct taaattgtcc gttacggctg
    76441 tagaacaccg aaccgttgct gtctaccaga cggaaaaaac cgttctggct gatagcaacg
    76501 tccagacctc gcccggtgtt ggtggtcgtg ccatcggtaa agtcctgagt gataccggca
    76561 acttttaccc ccagtcccac tttcgaaccg gcaaacatat cggcaaaaga ggccgtgcct
    76621 gatttaaagc cgtaggtggc ggagttggcg atattgttgc caataacatc gaggttggtg
    76681 gcggcagcgt ttaatccgct aaccgcttga gaaaaggcca tgactgactc ctgaaatgtg
    76741 aaggcttaaa ttatctgccg tacttcgtcg agggtggtgg tgccgtaagt gccgagatcc
    76801 agcgtattac cgctgttgcc gcggatcaca ccctgcacca gagcaaactg cagcggctgg
    76861 gcaaccagtt gtgtaccacc gttactggcg ctaatcgcta cattgtaaga accgttcggc
    76921 gcagtgctgc catcagtcaa cgaaccgtcc caggtgaaac tgtgaactcc ggcggtcagt
    76981 tcaccaatat caatggtgcg cacaaccgcg ccatttttat cggtgatggt ggccgtaact
    77041 ttgtctgccg cctgttgcag ctcaacacca aacggcgtgg tcgtggtcac agccccttct
    77101 tcactgccgg ttcccgcaag aacagttgtg ccggggatca tcacgccgtg accgatcagg
    77161 ttactggcct gtaacgactg gctgttatca atctgtccgg aaatagatcc gagcgtggta
    77221 ttgagttttt caatcccgct gaccgtgctg atttgtgcca attgcgacgt cagctcgttg
    77281 ttttccattg gattggtcgg gtcctggttt ttcagctgcg ccaccagcaa agtcagaaaa
    77341 ctgctttgta aatctgcggc gttgctgccc gtgagcgaac tactgctggt ggtactgacg
    77401 ccggtatttg tcggatcggt ggtggttacc gcaatggaca tagctttctc ctttattgac
    77461 cgagcgtaag ggttttcagc atcatgcttt tcaccgtgtt gagcacttca acattggcct
    77521 gatagctgcg tgacgccgac atggtgttaa ccatctcccc gacgacatca acgttcggca
    77581 tttttatgta gccctttgca tctgccagcg gattacctgg ttcataaacc agtttgtccg
    77641 gggcctgact ttcttaacat cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc
    77701 gttaacctgg aataccacct gttttgcccg atatggctgt ccatcaggac cggtcacgct
    77761 atcagcattt gccagattac tggccgccac gttcaggcgc tgggactggg cagttaacgc
    77821 cgacccggcg atatcaaaaa tattcagcag tgccatccgt taatttccgc tctgtaaaac
    77881 gttcatcatg cctttgattt gcccgctcaa cgcgctaagg ctcatctggt attgcaggct
    77941 gttatcggca aactgggtgc gttcgcgatc catatcgacg gtattaccgt caagcgaagg
    78001 ctggtccgga atacggtatt gcagttctgc ggtaggaggc gtcagcgcct gcgccgggat
    78061 gtgctgcgtt gaggtcatcg tcagtgcaac cacactggtt gcatcccgtc cacgttgcat
    78121 gactttttta agttcactgg caaaatcgat atcgcgcgcc tgataaccag gggtatcggc
    78181 attggcgatg tttgctgcca gcacttcctg acgctgggcg cgcagattga gcgcctcttg
    78241 ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct cctccgcagg tatcaaaatt
    78301 ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc ggaataaacg caaaatgggt
    78361 cgctatttat gccgttgatg gtcattgcgg acaggtacaa ttcacgttgt agaaatggct
    78421 gggggcgaaa atgctgacaa taaaacgtag cgtggcgatc atcgcgatac tgttcagtcc
    78481 gttaagtacg gcgagcaatc tcacatcgca attgcacaac ttttttagcg cccaactcgc
    78541 gggggtaagt gatgaggttc gtgtttctat tcgtacagcg cccaatctac taccgccatg
    78601 cgagcagcca ttgctttcga tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt
    78661 ggcacgctgc ggtaacgaca aacgatattt acaggttaat gtgcaggcca caggaaatta
    78721 tgtggttgcc gcgatgccta ttgtgcgggg aggaaagctg gaagctggca atgtcaaact
    78781 gaaacgtgga cgcctggata ccctgccacc gcgtacggtg ctggatatca atcaacttgt
    78841 tgatgccgtt agcctgcgcg atctatcacc cgatcaacct atccagttaa cccagtttcg
    78901 ccaggcatgg cgggtaaaag cgggacaacg cgtcaatgtg atcgccagcg gtgatgggtt
    78961 tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc gtcgcacaga atgcgcgggt
    79021 gcgcatggta tcggaacagg tagtcagcgg cgttgttgat gcagatggga atattcttat
    79081 aaacctgtaa gctgttaaag attacccgtc ccttgccgat aaataagcaa cacatgataa
    79141 aagcgccctc aatgaggaat aaaccatgag tattgatcgc acttcgcctc tgaagcctgt
    79201 aagcaccgtt caaccgcgcg aaaccaccga cgcgccggta acgaacagcc gggcggcaaa
    79261 aacaaccgcc tccaccagca ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca
    79321 acccggcagc agtgatatca atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa
    79381 cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca
    79441 agacttgcag agtaactgat cgtatgacac gtcttgcaga gatcctcgac cagatgtccg
    79501 ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca gcaacatctc tctatggggc
    79561 agatcaacgg cagccagttg caatggatta cagaacaaaa aagctcactg ctggcgacgc
    79621 tggattacct cgaacagtta cgcaagaaag aacccaatac agcaaatagc gttgatatta
    79681 gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact acgccaaatg aatcaacata
    79741 acggctggtt actggaagga cagattgagc gcaatcaaca ggcgctggaa atgttgaaac
    79801 cgcatcagga accgacgcta tatggggcga acggtcagac ctcaacaccc catcgcggca
    79861 gtaaaaagat ttcgatctga atattatctc cggcctgcac tgcaggccgg aatgcattgt
    79921 tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca gtaccgccag tgcagcgaag
    79981 tacgcggcaa tccccgccag cacgaccgcc attaaacgca gtaagcgcca gggcatggta
    80041 cccaatgacc actccggcat gatatgtaac atacctaaaa gcacgccaga catcaccagt
    80101 accgccacca ccagacgcaa cagaaacgcc atccagccgg gttgcggagt aaagattttc
    80161 tgcttacgca actgccagta aagcagcgaa gcattcagac acgccgccag accaatagaa
    80221 agtgacagcc cggcatgttt caacggacca ataaacgcca ggttcatcaa ttgcgttaaa
    80281 atcagcgtaa cgatggcaat tttcactggc gttttaatgt cctggcggga ataaaagcca
    80341 ggagccaaca ctttcactac aatcaggcca atcaaaccca ccgagtaggc aattaacgcc
    80401 cgctgggtca tcagcgcatc aaacgcggta aatttaccgt actggaacag cgaaacggtt
    80461 aacggacctg aaagaatgcc caacgcaacc gcactcggca gtgccaacag gaaacaaaga
    80521 cgcaaccccc agtccatcaa acggttgtat tcatcatgat tgccactggc aaaacttttc
    80581 gacagcgacg gcagcaaaat ggtgccaagc gccacgccca gcacaccgga gggaaactcc
    80641 attaagcggt cggcgtaata catccaagac accgaaccgg aagcaagaaa cgaggcaaaa
    80701 atggtgttga tgattaagga gatctggctc acagagacgc caaggatcgc cggtcccatc
    80761 tgtttcacca cgcgcattgc tccagcatcg tggaagttaa tgcgcggcag gaccagcatg
    80821 ccgatcttct tcaggtgcgg tagctgatac accagctgca gaatgccgcc gaccgtaaca
    80881 gcccacgcca gcgccagcac cggtgggtta aagtacggtg cggcaaacag cgcgaaacca
    80941 atcatgctga tgttaagcag tgttggcgca aacgccggaa tcgagaagcg gttccacgta
    81001 ttcagaatcg ctcccaccag cgacgccagg gagatcagca agatataggg aaaggtaatc
    81061 tttagtagct ggctggtcag ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc
    81121 accatgatca cccacggtgc ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt
    81181 gtcagcaggc cagaaacata agagacaaag acccgcgtgg cgtcttcacc ctgcttactt
    81241 ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg
    81301 cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca
    81361 aagattctgg cgacaattgc gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc
    81421 gagctgacgg cggccagcga ttttaataaa ttcatcggtg ttctaatccg gacccacggg
    81481 caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt ctacctagat tatggtgaat
    81541 tgctaccgcc agatgttaca gggtttattc actcatcgca tcgcgccaga tcttgtcaac
    81601 gatacgttgc gccagcactg cctattcgcc ggcggtttgc ggaactgtct gattttacac
    81661 gcattcaatg aagtgccgcg cacagccgac aaatccacgt tgctcaagag tgctctgcca
    81721 accaggaatc ggtttattca ctacaccctg cccgcgctcc tcacgccatt cgcgcatatc
    81781 cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg gtttcacgct gacttccggc
    81841 acggcgatgc atacaagtgg tgatctgcaa aggaccggcc gaaaaatggt gctcggcaaa
    81901 cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca tccagagagg ctttgccgcc
    81961 cgacaaccac agcgcggtat ccaccacatg cagataatca tccagcaacg tgaaataaag
    82021 atcgtgtggc ccgacgctat tgctacgatg tttatccatt cttagcgagg ctgcggtggc
    82081 gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa
    82141 cgtcagtttt ttccgcgccg ccagttccac cagcctttca gcatcgcgca gattttctgc
    82201 cagcggttta tcgacacaga catgtacccc cgcattgagt aacgtactga ccacgtcaaa
    82261 gtggctggcg gtgctggagt gcacaaaaac cgcatcacaa ctggcggcaa ggctggataa
    82321 cgaatcggca taaggaatgc gccagctgtc acaaattggc agggctttgg cgcgcgtagg
    82381 cgaccaggct ccttgtaacg tccagtcaga cgctgccgcc agcaccggta accacgcttt
    82441 ttgcgcaatg ccgcctaatc ccactacgcc gatacgtaat tttttcactt taatctccca
    82501 gatgggccag caacgaatca agacgttgtt tcagttctgc cacttcgatt tccagggctt
    82561 cgacgcgggt ctgtaaatca ccgtcaacca tgttcgacat agccgttacc gccggctgat
    82621 cttcaacctc accactgaaa agatgcatgt agcggctttc gcgtttaccc ggttcgcggg
    82681 ccagacgcac cacaaaagga ccatcttcgc gattcgccag ttgttccagc gtcgactcca
    82741 cttccgccat atcgctgaat tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg
    82801 tctgggcacc acgcaataac aacgtggtga ttaacgccac ttctgcggcg ctcagtttca
    82861 gatcgccaaa ttctgaatta caaaaacgtt gctcatattt ggtgacccga ttaccaaaac
    82921 cgctcactgt gcgtagataa tgacgtttga ccagattatc cagctgttcc tgcacttcgg
    82981 attcactcag attcatgacc ggttcacggt tcgttttctg attacaggcc gtgactacac
    83041 cattgactga gagcgggtat tgctccggcg tggtcacctg cttttccagc aagcaaccaa
    83101 ttacgcgagc ttccagggca gtaagttgat atttcatgag attctcctta gcggccgggc
    83161 gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac
    83221 agatagtctt tcgcatagcc ttctttttca aaacccagtc gcgccagtaa atcaccgctg
    83281 cgtttattgt gcggcatata attagccata atgcgatgaa tatgttgggt gcgctgcata
    83341 taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt tgccctgcca tttttgccca
    83401 atcgaataac cgagatagca ggcatgaaaa gagccgcgaa caacattgga aaaattggca
    83461 acgccaataa tctctttttc gtccgggtcg aataagccaa agtagaaagc tgaaccttgt
    83521 ttatgaaatt cgttaatcat ccctagcctg gcctgccagc ctgatggata acagtggctt
    83581 tcgtcgcgca ctggctccca gggcttgagg aaatggcgat tctctgcgta ataatccgcc
    83641 agacgccagg catcacgatc atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc
    83701 acttttggca cgttactgcg atagccaaac attctatacc tactccttcc cgtaacgtct
    83761 ttaatacgct gtaagtatta ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg
    83821 ccattttctg cattattcac atatttgata aaaactctct atcaaagcga cgttttgata
    83881 aaaaaatact gtccccgaag tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg
    83941 ctcatctttt atttcccggg aggggaaatg tcccgcgtat cgcaggcgag gaacctgggt
    84001 aaatatttcc tgctcatcga taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg
    84061 ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg ccgtcatggt cggtattgct
    84121 ctcggtctac gccagtttat tcagcaaggt ctgggtattt tcggcggcgc aattgccgac
    84181 cgctttggtg ccaaaccgat gattgttacc ggtatgctga tgcgcgccgc cggattcgcc
    84241 acaatgggta tcgcccacga accgtggcta ttgtggtttt catgcctgct ctcgggactc
    84301 ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa
    84361 caacgtggtc gttttttctc gctgttgatg atgcaggaca gtgccagtgc ggtcattggc
    84421 gcattgttgg ggagctggct gttgcaatac gactttcgcc tggtctgcgc cacaggggcc
    84481 gttttgtttg tgctatgtgc ggcgttcaat gcgtggttgt taccggcatg gaaactctcc
    84541 accgtacgca cgcccgttcg cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc
    84601 acctatgtcc tgacgctggc gggttactac atgctggctg tacaagtgat gctgatgctg
    84661 ccaattatgg tcaacgacgt ggctggcgcg ccctctgccg ttaaatggat gtatgccatt
    84721 gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc gctggagtga aaagcatttt
    84781 cgtctggaac accggttgat ggctgggctg ttgataatgt cattaagcat gatgccggtg
    84841 ggcatggtca gcggcctaca acaacttttc accctgattt gtctgtttta tatcgggtcg
    84901 atcattgccg agcctgcgcg tgaaacctta agtgcttcgc tggcggacgc aagagctcgc
    84961 ggcagctata tggggtttag ccgtctgggt ctggcgattg gcggcgctat tggttatatc
    85021 ggtggtggct ggctgtttga cctgggcaaa tcggcgcacc agccagagct tccgtggatg
    85081 atgctgggaa ttattggcat cttcactttc cttgcgctgg gttggcagtt tagtcagaaa
    85141 cgcgccgcgc gtcgtttgct ggaacgcgac gcctgatttg atactccccc ctgactcctc
    85201 catactgaaa gtgaactgac agccaaatgg aggagtcacg tgaagctata catttacgat
    85261 cactgccctt actgcctcaa agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa
    85321 ttacatgttc tgctcaacga cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag
    85381 gttcccattc tgcaaaaaga tgacagccgc tatatgccag aaagcatgga tatcgttcac
    85441 tatgtcgata aactcgacgg caaaccgtta ctgaccggca aacgttcccc tgccattgaa
    85501 gagtggctgc gcaaggtcaa tggctacgtc aacaaactgc tgttgccgcg ttttgccaaa
    85561 tcggcatttg atgagttttc tactcccgcc gcgcgcaaat atttcgtcga caagaaagag
    85621 gccagcgcgg gtaattttgc cgacctgctg gcccactctg acggtctgat taagaatatc
    85681 agcgatgatt tgcgtgcgct ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa
    85741 ctttcggaag atgatattca gctatttccg ctactgcgta atctgacgct ggtagccgga
    85801 atcaactggc caagccgcgt tgctaattac cgcgataata tggcgaaaca gacacaaatc
    85861 aatttgttat catcaatggc gatttaatcc tgacccgccg cggcagcctg tattcgcggg
    85921 ttgccgccgt tttcagtttt ttctgctggc gagtttatcg ctggtgacac atgctgcgtt
    85981 gtgtcaggat attgacgcag atttcatgtc gaggactacc atgaacaagt ttttatttgc
    86041 cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat caactcaccc aatacaccat
    86101 caccgaacaa gaaattaacc agtcgcttgc gaaacataat aatttctcaa aagatatcgg
    86161 tttacccggc gtggctgacg cccatattgt tctgacaaac ttgaccagcc aaattggtcg
    86221 cgaagagccg aataaggtta ccctaaccgg agacgccaat ctggacatga actccctgtt
    86281 cggtagtcag aaagcgacca tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga
    86341 aaaaggtgcg atcttcctga aagagatgga agtggtcgat gcgacggtac aaccggaaaa
    86401 aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac caggcattac gcaattactt
    86461 taaccagcaa cctgcttacg tcctgcgcga agatggcagt cagggcgaag caatggcgaa
    86521 aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt gtcattccgt ttacggatta
    86581 atgacgaggg cgcattcgcg ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc
    86641 ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag agattaatga ctgcaccatc
    86701 ccaggtatta aagatccgcc gcccagacga ctggcacctt cacctccgcg atggcgacat
    86761 gttaaaaact gtcgtgccgt ataccagcga aatttatgga cgggctatcg taatgcccaa
    86821 tctggctccg cccgtgacca cagttgaggc tgccgtggcg tatcgccaac gtattcttga
    86881 cgccgtacct gccgggcacg atttcacccc attgatgacc tgttatttaa cagattcgct
    86941 ggatcctaat gagctggagc gcggatttaa tgaagccgtg ttcaccgctg caaaacttta
    87001 cccggcaaac gcaaccacta actccagcca cggcgtgacg tcaattgacg caatcatgcc
    87061 ggtacttgag cgcatggaaa aaatcggtat gccgctactg gtgcatggtg aagtaacaca
    87121 tgcagatatc gacatttttg atcgtgaagc gcgctttata gaaagcgtga tggaacctct
    87181 gcgccagcgc ctgactgcgc tgaaagtcgt ttttgagcac atcaccacca aagatgctgc
    87241 cgactatgtc cgtgacggaa atgaacggct ggctgccacc atcactccgc agcatctgat
    87301 gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg cacctgtatt gtttacccat
    87361 cctcaaacgc aatattcacc aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg
    87421 agtattcctc ggtacggatt ctgcgccaca tgcacgtcat cgcaaagaga gcagttgcgg
    87481 ctgcgcgggc tgcttcaacg ccccaaccgc gctgggcagt tacgctaccg tctttgaaga
    87541 gatgaatgct ctgcagcact ttgaagcatt ctgttctgta aacggcccgc agttctatgg
    87601 cttgccggtc aacgacacat tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag
    87661 catcgcactg actgatgaca ccctggtgcc attcctcgcc ggggaaacgg tacgctggtc
    87721 cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg ttattttacc tgtataaata
    87781 accagtatat tcaacagggg gctattatgc gaattgaagt caccatagcg aaaacttctc
    87841 cattgccagc tggggctatt gacgccctgg ctggcgaact ttcccgccgt attcagtatg
    87901 cgtttcctga taatgaaggc cacgtatcgg tacgttatgc cgcagcgaat aatttatcgg
    87961 ttattggcgc aacaaaagaa gataaacagc gcattagcga aattctccag gaaacgtggg
    88021 aaagcgccga tgactggttt gtcagcgaat aatatgcagt gatttttgtt tttgccggat
    88081 cgccccggct tttttatttt acttacgaat tttgagtaca aaatttctac acttcctcaa
    88141 aataattgca caatatgcta aaaaaatatg aatgacatgc tgttttattg ttcatataaa
    88201 tcttaaaagc agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta
    88261 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
    88321 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
    88381 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
    88441 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
    88501 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
    88561 ggtgatttcc cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
    88621 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
    88681 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
    88741 ggttccgtag gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg
    88801 accgacgccc atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt
    88861 cgccatgctt atggtgaagg cgaaaagtat gtcccgttgg tcctccgcgc gcaaacgctg
    88921 tgggatgaac tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
    88981 aacctcggcc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
    89041 caactcaacg ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc
    89101 gtcccggaca actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg
    89161 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc
    89221 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag
    89281 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag
    89341 ctgcctgtcc agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc
    89401 gtgaagaata aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt
    89461 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
    89521 tcagcggatg aacgtgttcc gtttgcggaa gtggccagtg atgggtcaga agccttcccg
    89581 ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat
    89641 gataattcgc ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg
    89701 ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct
    89761 gccgattttg cgcaagacca aaaaagcgat tttgatttga cgccattcag gctttcccgc
    89821 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
    89881 gtcggctgtt gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc
    89941 tctggggatt gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac
    90001 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
    90061 tattcctgct tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga
    90121 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
    90181 atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtatt
    90241 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
    90301 ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac
    90361 gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat
    90421 tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
    90481 tccgctgccg agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt
    90541 gtgcctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
    90601 accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca
    90661 ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtctgt
    90721 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
    90781 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
    90841 tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat
    90901 tgacaaagaa ggtcagcacg cctttgttaa cttccgcata cagcatcttg gctatagctg
    90961 gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccggc
    91021 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaacc acgccgaacg
    91081 cgataaacat cttcgcagtg cggattttct caataccaca aaatatccac aggcaacatt
    91141 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct
    91201 gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc
    91261 atggggcggt aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa
    91321 tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg
    91381 cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta
    91441 ttctgttgga tcaggaatac ccagtgttgt attcagacgt ccacgagact tattaaagat
    91501 cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct
    91561 ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcgcata ctggacactg
    91621 aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcgcc
    91681 gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa
    91741 aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta
    91801 ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc
    91861 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
    91921 tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc
    91981 gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag
    92041 tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt
    92101 gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat
    92161 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc
    92221 taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
    92281 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
    92341 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
    92401 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
    92461 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
    92521 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
    92581 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
    92641 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg tataggttga
    92701 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
    92761 attggttaac ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccga
    92821 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
    92881 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
    92941 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggtatgg
    93001 cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca
    93061 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
    93121 tggagctggg cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
    93181 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
    93241 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
    93301 ggtacgcacc agatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg
    93361 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
    93421 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc
    93481 caccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
    93541 tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta
    93601 aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga tgcttcagaa
    93661 tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
    93721 cgccccctgg aagtcgggcg cataattagt gtgtttatct tttcttctta tgttcaccgc
    93781 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
    93841 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc
    93901 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg tgttaccggc cactccgccc
    93961 tgaatatgtg gtccggtatt gtcttcagta taatgggttc ctaaacacat cattttaggt
    94021 attgaatatg agacgggcaa tcagctacat cagattttca agtgaacgac aactgaaagg
    94081 ggactctgta cgccgacaat ctaagctggt tactgactgg cttgataaga accctgagtt
    94141 ttacttggat tcatccttga gctttaaaga tttggggaaa agtgctttta gtggtaagca
    94201 cctgaaaggt ggtctaggtg actttttaac cgctatcgag aaagggcttg ttaaagctgg
    94261 tgatacgcta ctaatcgaga gtttagaccg tctgtcacgt caggatattg atattgcttc
    94321 tgagctatta cgccgtatct taagggctgg tgtagatgta gtcacactga gcgatggaga
    94381 acattacaca cgagaaagtt taaaagaccc gtt
//
LOCUS       DAJTYL010000002        72846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYL010000002 DAJTYL010000000
VERSION     DAJTYL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.002"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO572_000107"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO572_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6659245.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO572_000108"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO572_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6659246.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO572_000109"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO572_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6659247.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO572_000110"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO572_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6659248.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO572_000111"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO572_000111"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6659249.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO572_000112"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO572_000112"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6659250.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO572_000113"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO572_000113"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6659251.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OO572_000114"
     CDS             complement(4523..5245)
                     /locus_tag="OO572_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6659252.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO572_000115"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO572_000115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6659253.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OO572_000116"
     CDS             6445..6663
                     /locus_tag="OO572_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6659254.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO572_000117"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO572_000117"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6659255.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OO572_000118"
     CDS             complement(7397..7597)
                     /locus_tag="OO572_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6659256.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO572_000119"
                     /pseudo
     CDS             complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO572_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO572_000120"
     CDS             complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO572_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6659257.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10026..11939
                     /locus_tag="OO572_000121"
     CDS             10026..11939
                     /locus_tag="OO572_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6659258.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSSEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            11939..12961
                     /locus_tag="OO572_000122"
     CDS             11939..12961
                     /locus_tag="OO572_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6659259.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            12955..13173
                     /locus_tag="OO572_000123"
     CDS             12955..13173
                     /locus_tag="OO572_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6659260.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13227..14096
                     /locus_tag="OO572_000124"
     CDS             13227..14096
                     /locus_tag="OO572_000124"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6659261.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14151..14555)
                     /locus_tag="OO572_000125"
     CDS             complement(14151..14555)
                     /locus_tag="OO572_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6659262.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14857..15489
                     /gene="crp"
                     /locus_tag="OO572_000126"
     CDS             14857..15489
                     /gene="crp"
                     /locus_tag="OO572_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6659263.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15540..17630
                     /locus_tag="OO572_000127"
     CDS             15540..17630
                     /locus_tag="OO572_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6659264.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO572_000128"
     CDS             complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO572_000128"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6659265.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO572_000129"
     CDS             complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO572_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6659266.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19598..20200)
                     /locus_tag="OO572_000130"
     CDS             complement(19598..20200)
                     /locus_tag="OO572_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6659267.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20190..20357)
                     /locus_tag="OO572_000131"
     CDS             complement(20190..20357)
                     /locus_tag="OO572_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6659268.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO572_000132"
                     /gene_synonym="rotA"
     CDS             complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO572_000132"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6659269.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21314..22495
                     /gene="tsgA"
                     /locus_tag="OO572_000133"
     CDS             21314..22495
                     /gene="tsgA"
                     /locus_tag="OO572_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6659270.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22757..25300
                     /gene="nirB"
                     /locus_tag="OO572_000134"
     CDS             22757..25300
                     /gene="nirB"
                     /locus_tag="OO572_000134"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6659271.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25297..25623
                     /gene="nirD"
                     /locus_tag="OO572_000135"
     CDS             25297..25623
                     /gene="nirD"
                     /locus_tag="OO572_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6659272.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25749..26555
                     /gene="nirC"
                     /locus_tag="OO572_000136"
     CDS             25749..26555
                     /gene="nirC"
                     /locus_tag="OO572_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6659273.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26574..27947
                     /gene="cobA"
                     /locus_tag="OO572_000137"
     CDS             26574..27947
                     /gene="cobA"
                     /locus_tag="OO572_000137"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6659274.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28194..28361
                     /locus_tag="OO572_000138"
     CDS             28194..28361
                     /locus_tag="OO572_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6659275.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28656..29993
                     /gene="frlA"
                     /locus_tag="OO572_000139"
                     /pseudo
     CDS             28656..29993
                     /gene="frlA"
                     /locus_tag="OO572_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30014..31036
                     /gene="frlB"
                     /locus_tag="OO572_000140"
     CDS             30014..31036
                     /gene="frlB"
                     /locus_tag="OO572_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6659276.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31086..31916
                     /gene="frlC"
                     /locus_tag="OO572_000141"
     CDS             31086..31916
                     /gene="frlC"
                     /locus_tag="OO572_000141"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6659277.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31913..32698
                     /gene="frlD"
                     /locus_tag="OO572_000142"
     CDS             31913..32698
                     /gene="frlD"
                     /locus_tag="OO572_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6659278.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32798..33529
                     /gene="phoB"
                     /locus_tag="OO572_000143"
     CDS             32798..33529
                     /gene="phoB"
                     /locus_tag="OO572_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6659279.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33689..34774)
                     /locus_tag="OO572_000144"
     CDS             complement(33689..34774)
                     /locus_tag="OO572_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6659280.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34786..36090)
                     /locus_tag="OO572_000145"
     CDS             complement(34786..36090)
                     /locus_tag="OO572_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6659281.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36102..36455)
                     /locus_tag="OO572_000146"
     CDS             complement(36102..36455)
                     /locus_tag="OO572_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6659282.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36466..37344)
                     /locus_tag="OO572_000147"
     CDS             complement(36466..37344)
                     /locus_tag="OO572_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6659283.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37341..38567)
                     /locus_tag="OO572_000148"
     CDS             complement(37341..38567)
                     /locus_tag="OO572_000148"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6659284.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38568..39731)
                     /locus_tag="OO572_000149"
     CDS             complement(38568..39731)
                     /locus_tag="OO572_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6659285.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39815..40177)
                     /locus_tag="OO572_000150"
     CDS             complement(39815..40177)
                     /locus_tag="OO572_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6659286.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40194..41099)
                     /locus_tag="OO572_000151"
     CDS             complement(40194..41099)
                     /locus_tag="OO572_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659287.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41369..42373)
                     /gene="trpS"
                     /locus_tag="OO572_000152"
     CDS             complement(41369..42373)
                     /gene="trpS"
                     /locus_tag="OO572_000152"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6659288.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42366..43124)
                     /gene="gph"
                     /locus_tag="OO572_000153"
     CDS             complement(42366..43124)
                     /gene="gph"
                     /locus_tag="OO572_000153"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6659289.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43117..43794)
                     /gene="rpe"
                     /locus_tag="OO572_000154"
     CDS             complement(43117..43794)
                     /gene="rpe"
                     /locus_tag="OO572_000154"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6659290.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43812..44648)
                     /gene="dam"
                     /locus_tag="OO572_000155"
     CDS             complement(43812..44648)
                     /gene="dam"
                     /locus_tag="OO572_000155"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6659291.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44755..46047)
                     /gene="damX"
                     /locus_tag="OO572_000156"
     CDS             complement(44755..46047)
                     /gene="damX"
                     /locus_tag="OO572_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6659292.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46139..47227)
                     /gene="aroB"
                     /locus_tag="OO572_000157"
     CDS             complement(46139..47227)
                     /gene="aroB"
                     /locus_tag="OO572_000157"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6659293.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47284..47805)
                     /gene="aroK"
                     /locus_tag="OO572_000158"
     CDS             complement(47284..47805)
                     /gene="aroK"
                     /locus_tag="OO572_000158"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6659294.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48206..49444)
                     /gene="hofQ"
                     /locus_tag="OO572_000159"
     CDS             complement(48206..49444)
                     /gene="hofQ"
                     /locus_tag="OO572_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6659295.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49356..49762)
                     /gene="hofP"
                     /locus_tag="OO572_000160"
                     /pseudo
     CDS             complement(49356..49762)
                     /gene="hofP"
                     /locus_tag="OO572_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49752..50192)
                     /gene="hofO"
                     /locus_tag="OO572_000161"
     CDS             complement(49752..50192)
                     /gene="hofO"
                     /locus_tag="OO572_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6659296.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50176..50715)
                     /gene="hofN"
                     /locus_tag="OO572_000162"
     CDS             complement(50176..50715)
                     /gene="hofN"
                     /locus_tag="OO572_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6659297.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50715..51494)
                     /gene="hofM"
                     /locus_tag="OO572_000163"
     CDS             complement(50715..51494)
                     /gene="hofM"
                     /locus_tag="OO572_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6659298.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51614..54166
                     /gene="mrcA"
                     /locus_tag="OO572_000164"
     CDS             51614..54166
                     /gene="mrcA"
                     /locus_tag="OO572_000164"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6659299.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54331..54891)
                     /gene="nudE"
                     /locus_tag="OO572_000165"
     CDS             complement(54331..54891)
                     /gene="nudE"
                     /locus_tag="OO572_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6659300.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55211..57346
                     /gene="igaA"
                     /locus_tag="OO572_000166"
     CDS             55211..57346
                     /gene="igaA"
                     /locus_tag="OO572_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6659301.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57411..58079
                     /gene="yrfG"
                     /locus_tag="OO572_000167"
     CDS             57411..58079
                     /gene="yrfG"
                     /locus_tag="OO572_000167"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6659302.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58090..58491
                     /gene="hslR"
                     /locus_tag="OO572_000168"
     CDS             58090..58491
                     /gene="hslR"
                     /locus_tag="OO572_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6659303.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58516..59385
                     /gene="hslO"
                     /locus_tag="OO572_000169"
     CDS             58516..59385
                     /gene="hslO"
                     /locus_tag="OO572_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6659304.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59529..61280)
                     /locus_tag="OO572_000170"
     CDS             complement(59529..61280)
                     /locus_tag="OO572_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6659305.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQGRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61659..63281
                     /gene="pckA"
                     /locus_tag="OO572_000171"
     CDS             61659..63281
                     /gene="pckA"
                     /locus_tag="OO572_000171"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6659306.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63357..64709)
                     /gene="envZ"
                     /locus_tag="OO572_000172"
     CDS             complement(63357..64709)
                     /gene="envZ"
                     /locus_tag="OO572_000172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6659307.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64706..65425)
                     /gene="ompR"
                     /locus_tag="OO572_000173"
     CDS             complement(64706..65425)
                     /gene="ompR"
                     /locus_tag="OO572_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6659308.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65653..66129
                     /gene="greB"
                     /locus_tag="OO572_000174"
     CDS             65653..66129
                     /gene="greB"
                     /locus_tag="OO572_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6659309.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66226..68547
                     /locus_tag="OO572_000175"
     CDS             66226..68547
                     /locus_tag="OO572_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6659310.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68985..69212
                     /gene="feoA"
                     /locus_tag="OO572_000176"
     CDS             68985..69212
                     /gene="feoA"
                     /locus_tag="OO572_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6659311.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69229..71550
                     /gene="feoB"
                     /locus_tag="OO572_000177"
     CDS             69229..71550
                     /gene="feoB"
                     /locus_tag="OO572_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6659312.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71550..71786
                     /gene="feoC"
                     /locus_tag="OO572_000178"
     CDS             71550..71786
                     /gene="feoC"
                     /locus_tag="OO572_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6659313.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71989..>72846
                     /gene="rpnA"
                     /locus_tag="OO572_000179"
     CDS             71989..>72846
                     /gene="rpnA"
                     /locus_tag="OO572_000179"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6659314.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEE"
BASE COUNT        17561 a        19745 c        18421 g        17119 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcaggc cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcatt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca cgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagagcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcaa aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag actcaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcgc ttgccggtga ggtcagttgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaacttatg gccttctgct
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg aaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacactag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct ttaagagagt tttccatgta acgacccagc
     6061 gaagcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgatgc tttgctgtct
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagtag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtggcc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ccttttcggt attagtggca
     6721 accacaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc
     6841 gccgtgaacg tggccatgag ccagttcttc ttcggtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca ctacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcagtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcactag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgtgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgctataaaa cgttttcgga ttgccatcct taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc agcatgcatc gggattaact
     7741 cccgcagcat tcgcatatcc aggcggcgaa aatgcagttg cgcccgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccatat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8341 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8401 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8461 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8521 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8581 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8641 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8701 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8761 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaactcc agcaccttca
     8821 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     8881 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     8941 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9001 tcgcaaagcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9061 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9121 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9181 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9241 acgggccaat tgcaatcccc gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9301 aagagaaaca gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag
     9361 acagcggatt tgccaaccag tcaccgtagg ctctggcgtg gctcgccagc tcctttgcgc
     9421 tttgccgtct cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg
     9481 ccgccagttc aaaggggcgc agcacatcgc tcatcgggta gcgattcagc gcgtcataac
     9541 ggtaagcact ttccggctcg ccggtggtaa tcacgttacg ccagtacttt cccgccagtt
     9601 ggtttcctcc cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca
     9661 gtagcgccgg gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc
     9721 gcagtaatgc ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt
     9781 cgtgcacggt aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg
     9841 agtcctgaga ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc
     9901 ccctcccggt atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg
     9961 gcagctcgcc catacattac attatcataa tgataagtta acatagtctg aacatacggc
    10021 gccttatgat tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata
    10081 atgccaccgc caccatcaac cccgggcaga aagtcggcct ggtgggtaaa aacggctgtg
    10141 gtaaatctac cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca
    10201 cctttccggg aagctggcaa ctggcgtggg tgaatcagga aacgccagcg ttaccgcaag
    10261 cggcgctgga atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctgc
    10321 acgacgccaa cgaacgtaac gacgggcacg ccattgcgac cattcatagc aagctggatg
    10381 ctattgacgc atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca
    10441 gcagtgaaca actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta
    10501 accttgccca ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc
    10561 acctcgatct cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc
    10621 tgatcctgat ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata
    10681 tcgaacaaca aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg
    10741 ccacccgtct ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtggcacatc
    10801 tgcaaagtta tatcgatcgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc
    10861 gcattaagat gctggaacgt atggagctga ttgcccccgc acacgtcgac aacccgttcc
    10921 gctttagctt ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaggtca
    10981 gcgcaggcta tggcgatcgc attattctcg actcgattaa attgaatctg gtccccggct
    11041 cgcgcattgg tctgttaggc cgcaacggcg caggtaaatc gacattaatc aaactgttag
    11101 ccggtgaact tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct
    11161 acttcgccca gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg
    11221 cacgtttagc gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt
    11281 tcctgggcga taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc
    11341 tggtgctggc attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta
    11401 accaccttga cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg
    11461 cgctggttgt cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc
    11521 tggttcacga tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt
    11581 tgagcgacgt acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca
    11641 gcgcccaggc acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc
    11701 cgctgcgtaa agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg
    11761 cgcaggcgga agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt
    11821 tgaccgcctg cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg
    11881 cgtggctgga agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat
    11941 ggcgcagata acgacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat
    12001 gcgcggcttt agcaattgtc atctacaaac tatgctgccg cgtttgtttc gtcgtcaggt
    12061 gaaattcacc ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc
    12121 gtggagtgaa gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct
    12181 ggaaggcagt ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg
    12241 ctggctgggc gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg
    12301 catttaccat tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga
    12361 atttggtcat gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg
    12421 tttgctggca aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc
    12481 gtttatgctg gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg
    12541 ttacttgctg aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac
    12601 gctgccgatt aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct
    12661 aatcaccgcc agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc
    12721 catgccaatg ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc
    12781 gtttatggat catcaggtga tcccaaaacc ggaaagtctc ccctcgcagg tggagtatca
    12841 actgactgaa catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat
    12901 gtggctggag tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg
    12961 attccgtggc aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta
    13021 cgtgaaggca ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc
    13081 aaacgccagc tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc
    13141 aatattatgc cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat
    13201 agtgatagct aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag
    13261 gatccagcgg cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt
    13321 taaatctgca tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa
    13381 tggacatggc gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg
    13441 aagcgaacga cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag
    13501 ggaaatctcg caaatatctg catacctacg acgaagccgt accctggaat caggtaccgg
    13561 gaacattcac cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt
    13621 tacacggcgg cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg
    13681 gcgtagtgcc tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc
    13741 gcgggcactc acgagaagca gtgatggact cggtagtgcg ttcaatggaa gactatatca
    13801 actacatcac accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg
    13861 acacttcaaa cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga
    13921 tccattttcg taatctggaa gggatcgatt tcccttggct gctggcgatg ttgcaaggct
    13981 cattcatttc ccacatcaat acgttagtgg tacctggcgg caaaatgggg ctggcaatgg
    14041 aattaattat gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaacttt
    14101 gctattatgc cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca
    14161 accacttcat acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa
    14221 tatttctcgg cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg
    14281 gtgacgataa aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca
    14341 gaggtcaatt ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc
    14401 acatcgatgg cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct
    14461 ttatcgcctg agttgccgtc cattaaaatc tggtgaccag aggcggattc gcccagaaaa
    14521 gttaaccctt cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt
    14581 tcctgacaga gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg
    14641 aagcgagaca ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt
    14701 aatacattga tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat
    14761 agccccttcc caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg
    14821 gctctggaga aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca
    14881 gacccgactc tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc
    14941 acgcttattc accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg
    15001 gcagtgctga tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt
    15061 gattttattg gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt
    15121 gcgaaaaccg cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag
    15181 gtaaacccgg acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact
    15241 tcagagaaag tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg
    15301 ctgaacctgg caaaacaacc agatgctatg actcacccgg acggtatgca aatcaaaatt
    15361 acccgtcagg aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg
    15421 aagatgctgg aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc
    15481 actcgttaat cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga
    15541 tgtggcgcag actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc
    15601 tatgtttgcc cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct
    15661 ccctcgttcc tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca
    15721 aacacttaat cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac
    15781 tggtaaaaga tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca
    15841 ctgctgagct ggggccatta cacgcaaaat tgcttcctgc atcgctgctc gccgccattt
    15901 ttaccctcag tctggcggga tacatgccgg tctgggaacc attgctcatc tatgcgttgg
    15961 gcactctctg gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc
    16021 tgcgcgagtc actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca
    16081 gcctgcttac ccagcacacc gatcctgaaa aagcgctgcc accgctgctg gtgcgccagc
    16141 aaaaagcggt cgatttaatt actcagtgct atcagcaaat gcatatgctt tccgcgcaaa
    16201 acaataccga ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttgcaggaac
    16261 atatttcggt cagtttgcat cagccggaag aggtgcaaaa actggtcgag cgtagccatg
    16321 cggaagaagt tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg
    16381 atgacattct ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac
    16441 tggaaaaaat cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt
    16501 tcagccgcat cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg
    16561 ccgataaaca gcggcgaatg ccgttgcttc cggcactgaa aagttatctg tcactaaagt
    16621 ctccggcgct acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg
    16681 gcaccgtgct gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac
    16741 aaaatggcta tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg
    16801 ggttaatcat tgcgggcgta gcgctgcact ttaaaattcc cgaaagttac accctgacgt
    16861 tgatgctgat taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcaa
    16921 cggtcggttt tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc
    16981 agtacatcct tccacgtctt atcgatacca ttattggttg tttgattgct ttcggtggta
    17041 ccgtctggct gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgatgctt
    17101 tagaagccta tcaggaagcg attcgcttga ttctgagcga ggacccgcaa ccaacgccac
    17161 tggcctggca gagaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc
    17221 aggcgatgca ggaaccggca ttcaacagcc attatctggc agatatgaaa ctgtgggtaa
    17281 cgcacagcca gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc
    17341 gggcattgcc acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc
    17401 gttgtcagca gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg
    17461 atgcgccgga gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc
    17521 gggttattgg ccatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac
    17581 cgcatcacgg gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tacaggctaa
    17641 accatcggat aaggcattca cgccgcatcc gacatatttt gcctgatacg tcttatttac
    17701 gccccaacca ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca
    17761 tcttccacca ccagcgacgg tgctaaacgc atcacatccg gtccggcatt cagcaccatt
    17821 acgccaacct ctgcacccgc atacaggaaa tcacgcgccc gacctttgta gtgtggtttc
    17881 agctccgcgc caattaacag ccccataccg cgaatatcgc taaacacatc gtactgctga
    17941 tcgatcttct gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact
    18001 tcaggggtat tgatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg
    18061 ccgtaggtgg aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc
    18121 atggcgctaa tcgggaagcc gccgcctaac gctttcgcag aagtcagaat atccggcgtc
    18181 acaccgtagt acctgtaagc aaacaaatcg ccagtccgcc ccatcccgca ctgcacttca
    18241 tcaaacacca ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct
    18301 ggcgtcgctg ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg
    18361 tgatcatcca tcaccgcttt cactgcatgg agatcgttaa agggaacgtg gatgatgtct
    18421 gccggtttcg gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac
    18481 agcgagcgac catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga
    18541 cgcacacagg cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag
    18601 ttcataaaca ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc
    18661 gccggttcat tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac
    18721 gcgttcacca gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa
    18781 tcgacatact ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata
    18841 aactctgccg gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct
    18901 gtttgttcaa ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa
    18961 tcacaaaata tgcataaaaa atcactgaat ggcaatcgga aatcagagat gcaggaaatt
    19021 agccagcagt tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc
    19081 cagatcccac tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac
    19141 gtcaaagcac gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt
    19201 aagtggattt gccagccccc gaaatacgcc ctcaccgcta tgtgtaatcg gcgaggtttt
    19261 gccgtgcatg acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg
    19321 atgaccgagg cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag
    19381 ggagatcccg gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt
    19441 aagggcgtcg atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc
    19501 cagttcacaa aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag
    19561 gatcatggcg gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc
    19621 ttaccacttt gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat
    19681 ttgcctgatt ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa
    19741 tcgccagttg ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca
    19801 gcacgttgat ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc
    19861 caaccagata accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat
    19921 aagcaaagtg gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg
    19981 cccagtcgaa aatatcctgg tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc
    20041 gcaccagcgg accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct
    20101 gttccagccg ctgctgctgg cggatgttca gccggttacg catgatatca aggccaggat
    20161 aaagatacag atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca
    20221 aggcgcgcta aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga
    20281 cggcgactgg cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta
    20341 tcggtgagtt tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg
    20401 gcaaatccgg gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc
    20461 attacggcag gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg
    20521 gaccaacatc atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca
    20581 ctttaccaaa taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg
    20641 caacgttgat aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga
    20701 tggtgccacg cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt
    20761 ttttctgctg catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc
    20821 ggtgaaaggt agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag
    20881 acactggcgc tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca
    20941 atacgtgcgg gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcagaaa
    21001 gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa
    21061 cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc
    21121 tttacctaat tttacgtatc tgaaatatat gtaacctgac gatcactttt tattccgtga
    21181 tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaaacagat
    21241 cataaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt ttatttttga
    21301 acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta gctttctctc
    21361 ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga
    21421 ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca acgccggcat
    21481 tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt
    21541 acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct
    21601 ggcgctgttc tcgacggcga tgttcattct cggggtggtc agcggcatta ctatgtcgat
    21661 tggtacattc ctgataacac aaatgtatga agggcgtcag cgcggttccc gcctgttatt
    21721 tactgactcc ttcttcagta tggctgggat gattttccca atgatcgccg cgtttctact
    21781 ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt atgtcgctat
    21841 ttttattctg accttcggct gtgagttccc ggcactgggc aaacgtgcac caaaaacgga
    21901 tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct
    21961 gtgctacatc ctcggtcagt taggttttat ctcctgggtg cctgagtatg ccaaaggcct
    22021 gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt catacatggt
    22081 cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgca ttctgaccgt
    22141 actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac cagtacatat
    22201 ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca ccatcatcac
    22261 cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg
    22321 cggaaccatc ggtactatgt tgacctttgt ggttaccggc acgattgttg aacatagcgg
    22381 tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt
    22441 cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgct
    22501 aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct acgtgcatct
    22561 atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg tcagacaatt
    22621 ccgtgactta agaaaattta tacaaatcag caatataccc cttaaggagt atataaaggt
    22681 gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa
    22741 agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg
    22801 ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt
    22861 tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcatctt acttctctca
    22921 ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa
    22981 agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag
    23041 cgccggacgt accgtttttt atgacaagct gatcatggca accggttcct acccgtggat
    23101 cccaccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct
    23161 caacgccatt gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg gtggcggcct
    23221 gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc acgttatcga
    23281 atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc agctgcgtcg
    23341 caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacacccttg agattgtgca
    23401 ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga
    23461 ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct
    23521 ggacgttgct ccgcgtgggg ggattgtcat taatgattcc tgccagactt ccgatccgga
    23581 tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc
    23641 aggctacaaa atggcgcagg tcgccgttga ccatattctg ggtagcgaaa acgcctttga
    23701 aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggtg gtattggtga
    23761 tgcgcacggt cgcacgcctg gcgcacgtag ctatgtttac ctcgacgaaa gtaaagagat
    23821 ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg
    23881 cgataccagc gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga
    23941 aaacccggat tctctgattc tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt
    24001 tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta aaggtgatct
    24061 gattgctgcc atcaacaaag gctgccacac agttgcggcg ctgaaagctg aaaccaaagc
    24121 gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg cggaactggc
    24181 gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt cgcgtcagga
    24241 actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca
    24301 cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc tggcctcctg
    24361 ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta acgacaactt
    24421 cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga
    24481 aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtgaattta atctctacac
    24541 caaaatcacc ggctcccagc gcctggcgat gtttggcgca cagaaagacg atctgccgga
    24601 aatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg cgaaagcact
    24661 gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt
    24721 cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa
    24781 gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag acgtgggtat
    24841 tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg
    24901 tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg
    24961 cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga
    25021 aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct
    25081 gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac
    25141 tgaaacggtc aatacaccgt ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa
    25201 gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta
    25261 tgaacgtatc ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc
    25321 aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcac tgttaggtga caagcaggta
    25381 gcgattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat tgatccgttc
    25441 ttcgagtcca gcgtgctatc acgcgggctg attgcggaac accagggcga gctgtgggtc
    25501 gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa
    25561 cagttttccg tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt
    25621 taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta atgaccttcg
    25681 gtatattgca ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg
    25741 ataatcaaat gtttacagac actattaata agtgtgcggc taatgctgcg cgcattgcac
    25801 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcatatg
    25861 tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    25921 cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg
    25981 aactgttcac cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc
    26041 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct
    26101 tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    26161 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    26221 cattgtgtaa ctggctggtt tgcctggcaa tctggatggc gctgcgcact gaaggggcgg
    26281 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    26341 ctatcgctaa catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca
    26401 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    26461 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    26521 acaaatttaa tcaaactgaa acagctgccg gttaattact aaggggtttt tacgtggatc
    26581 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    26641 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    26701 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    26761 ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg acggatgatg
    26821 acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag
    26881 ttgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctaa
    26941 tggtagcggt ctcctctggc ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac
    27001 tcgaatcact gctgccgttg catctgggcc aggtggcgaa atatgccggg caattacgcg
    27061 ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt
    27121 tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa
    27181 cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg
    27241 caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag
    27301 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    27361 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag gaagagatta
    27421 accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg
    27481 atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc
    27541 cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    27601 cactcacgca tcgcgattat gcccagagcg tacgcttaat taccggacac ttaaaaaccg
    27661 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg
    27721 ggttgaatca ggccgcgact attcagcaaa agctgattga acacggtatg cctggcgaaa
    27781 tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc
    27841 tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt attattggtc
    27901 gggttgttgg cctgcgcgat aaactgaact ggttctccaa tcattaattt aacccgggcc
    27961 agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc
    28021 ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgattta caaaattaag
    28081 tcattcgctc actgacgcca attaaatatt cctgtatcgt tcttaacgag cagtctgctt
    28141 atattcatat aatcaatgaa tattaattaa tatataatac atagggaatg taaatgaaca
    28201 aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc
    28261 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta
    28321 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
    28381 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
    28441 tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttta taactacatc
    28501 cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat cacataacat
    28561 aatatgttgt tatattcatc atgcattgtc atgttacctt ttaaatggct gcaaactctc
    28621 ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaggaa ctccaacgca
    28681 agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt
    28741 ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt
    28801 ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg
    28861 cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg
    28921 ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg
    28981 cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat
    29041 ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg
    29101 gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg
    29161 gccttgggat cttctggttt aaagcggaga attttgccgc ccttaccacc actgcgattg
    29221 gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata
    29281 ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc
    29341 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
    29401 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
    29461 ccctgaccta gatccccgca ctcggcagca ccgctgggct ctttgttgcc atcacggcga
    29521 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
    29581 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgcgcatccg aaatacaaca
    29641 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
    29701 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
    29761 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
    29821 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
    29881 ttgtctgggc accgattccc ggccttatct gcgccgccat cgttattgct actggtctgc
    29941 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
    30001 tgaagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
    30061 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acgccatcgt
    30121 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gctcgccact
    30181 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
    30241 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
    30301 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
    30361 ggcctgcgga gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
    30421 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
    30481 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
    30541 caaaaacgat ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca cctgggaaga
    30601 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
    30661 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
    30721 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
    30781 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
    30841 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
    30901 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
    30961 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
    31021 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccggatttac gcaaggagtt
    31081 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
    31141 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
    31201 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
    31261 atcagatgcc gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc
    31321 tgggcgatga acatatgcgt cgtgaaagcc tggacatgat caagctggcg atagatatgg
    31381 caaaagagat gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc
    31441 cgcctaacgt tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg
    31501 aaaacatcgg catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
    31561 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
    31621 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
    31681 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
    31741 acattcctgg agaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg
    31801 gctatgaggg ttactctacg gtggagctgg tgacgatgta tatgaacgag cccagactct
    31861 atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac
    31921 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
    31981 tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg
    32041 cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat
    32101 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
    32161 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
    32221 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
    32281 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
    32341 cttctccgac aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc
    32401 ctttgcctcc gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
    32461 ccgtggcgca ggaacagtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg
    32521 cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg
    32581 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
    32641 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
    32701 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
    32761 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
    32821 tcatcaactc ctctacgcta ccgtccgcca gcggctgctg gatgatatcg cgcagggggt
    32881 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacacaat ataacgtcag
    32941 ccgcattacc attcgcaaag ccatcagcga cttagtggca gacggcgtac tgatccgctg
    33001 gcagggaaaa ggcacctttg tacaaagcca gaaagttgaa aacgccctgc ttactgtcag
    33061 tggttttacc gattttggcg tctcacaagg caagtcgacg aaagagaaag tgatcgaaca
    33121 ggaacgggtc agcgccgcgc cgttttgcga aaagctgaac atccccggaa acagcgaagt
    33181 gttccatctc tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcatg
    33241 gatcccgctg tcgcgttatc ctgactttga tgagatttac gtcgaaggaa gctccaccta
    33301 tcagttattt caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat
    33361 ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt
    33421 tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt
    33481 ctgccgcgcc aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg
    33541 ataagatgcg ccagcatcgc atccggcgat gctggcgcac tgaattttac atcccgcacg
    33601 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcagtg cggaatttag
    33661 cttgattgcc gaacggtgct atccagcatt aaatactcgc aatactctca cgcaaaatcc
    33721 gcagcaccgt ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttcgagtt
    33781 gtggattcgc ctggcgaaac gttcctgaaa gtcgatagaa gagcggcggg atttcatatt
    33841 tcgactctgc accgaccggg taaggcaacg cgccacgctt ttgcgcttct tccagcaccc
    33901 tggcggcaat cagctgatga aactcgacaa tcaacacctt cgactgcgca ttggcaatga
    33961 cggcgctttt cacttccgcc accgcgccgc cgttaagcaa agccagcaac cgttcggata
    34021 ctcccgcctg caccgcatgc atcactggcg cgaacaccag accacgcaag acttccagcg
    34081 cctgtgagcc ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaatc cgactaataa
    34141 catcggcatc gccaaccact gcaccaacgc cctccggccc aaacagcttg aagcaggaga
    34201 atgtcgagac attcgcgccg cattcacagc cgattcgcgc taccttcatc accgcatagt
    34261 tatcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca
    34321 catagctgtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct
    34381 cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc agcagtaata agcgtcagcc
    34441 ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac
    34501 gctgccccgg tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct
    34561 gcaccagcgc cgcggcctgt gcgtgaaatg catcagccag cacctgctcc acccgttggg
    34621 taatacgcgg ttgattcaga cttggagtta accctaaatc accgccagca agaaactcgc
    34681 tgcctggaaa atggcggcaa atagtgtcca ccagcgcaaa ctgcttttgc tgcgcttcag
    34741 caagagtcag gctttgcaaa ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
    34801 caggccaagc cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg
    34861 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
    34921 gatagagaat ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag
    34981 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga
    35041 cgggtagcgt cccagccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
    35101 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac
    35161 aaaggtaaag cccgcaactg catacacacc cgttgctaag gcggtggtgg caatcaacgg
    35221 cacaaacccc agtccgcgca taaattctgc cagagccgcc tgattaatca gcgtttgcga
    35281 ttgttccggc gtcacgcctg cggaatacgc tttctccagt gtgaagatcg aaacttcgct
    35341 accagcaaaa atcttcatac tggcaacggc ggcaatcaat gctcccacga tggcgatata
    35401 aggtaagttt ttgataatcc gcgacgtgcg ctcttcaaac accgaaagcc cgctggcatc
    35461 gatgtcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac
    35521 catgccaata aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac
    35581 cacacgggtc atcagaacaa ccacggcggc gaccagactt tgcttccagc caaactggta
    35641 gaaaatcgcc accagcggga acagtgcaaa agcagaaacc accggcgagc ttaattcccc
    35701 gaggctacct aatacatcca ccggcagcgc ggtcagcagc tggttaactg gcaacaggca
    35761 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
    35821 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt
    35881 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
    35941 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggtggatgcc
    36001 gtcatgaaaa acagccgccg agcgatgctc cagaagcgat gtcatacccg tcaggcacgc
    36061 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
    36121 aacatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac tttcccttca
    36181 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
    36241 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
    36301 cacgcgccaa tgtaataatc tagttgcccg gatttcacct tcattgccgc ttccatgtcg
    36361 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc
    36421 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
    36481 agagggattt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
    36541 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg
    36601 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag
    36661 cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc
    36721 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag
    36781 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acagagccag
    36841 ttgttccagc cccatggtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt
    36901 atgcgccagc gcagcggcaa taaatacctt ctcttccagc ggcgtaatct ttccttcgct
    36961 ggagccgatc tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat
    37021 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcaccacat gttccgggaa
    37081 aaacgcatcc tggtaataac cagtacaggc caccacgttg atcccggttt cgcgcattac
    37141 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
    37201 tacaccccgg gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
    37261 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggccagggt
    37321 gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
    37381 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc tcccacatca
    37441 gaaagcgtgg tgcgcacacc gagctgcgtg gcgaccagcc cttgctgata aaccagcact
    37501 ggcaccactt cacgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
    37561 atcacgacca ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca
    37621 acgacctgca aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
    37681 tcctggatat tgatgcaaat aaacgccgtc ggatgggtgt taaattcgtc gagggtgata
    37741 tccataatcc gctggctatc caccaggttt tgccagctca caccataagg attgctgacg
    37801 atatctgcca ccttacccac cagcacggtt ggcacgcccg cttcatacag tttttgcggc
    37861 acctgcactt tttcatcaac accgtagccc atatgcacga cctggaagcc gttgtcataa
    37921 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
    37981 agaatacgct gactgttggt taacaggccg ccaaatgtaa tgacccgacc gacctgtacc
    38041 tggtcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
    38101 gcggtaatgt tatagacttg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
    38161 tgattgaccg acagaaattg cagatcatcg ccacggcgct ccacctgcca gccagcggaa
    38221 actaatgcct gctcaacacg gtcaatcaca tcgcgaaaag gcatccgcag cggcggtaac
    38281 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
    38341 tctgccacgc cccaggttgc ggaatctgac ggctgcatat cgcctggcgc ataacccaat
    38401 gtgttgatta gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg
    38461 atgtgaccac aggtattcgc cccggcatct tgcgggcgca ccagcgtgac atctttcatt
    38521 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattta tgcctccaga
    38581 gggttgccga ggctgtcata acgaccaact atttctggtt cgccgcgctg aataccggac
    38641 accagcacca catcgctacg ggtgacaaaa atctgcgtgc gaaaacagag caccactgca
    38701 ctgctcaccg gatactcgcc tgccagcggc agggtgtagt cgatactgct gtcatccaca
    38761 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
    38821 gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
    38881 gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg ctggtttgcc
    38941 ggaatagtac cggtcagcgc atgaccgggt tcggcatgag tcgcaccgta ttccgccagc
    39001 aatggcagcg aagtgcagct ggtcgctgaa ggtacgttca gttgctcaat cgcaatacca
    39061 gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt tggcaaaact
    39121 ttcccagcag cctcatccca aagcaggcaa gggaaatggg ttagtccggc taaatgcagc
    39181 cccggcagtt tcttaatttc agcaatcacc tcgtgcagcg aatgctgtac aaaaccgctc
    39241 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac aggctggacc
    39301 cgtccggcct taaccgctgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
    39361 atgatgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat ttgtaccaga
    39421 tgcccctgat gcgccactgg caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
    39481 accgccacaa tgccgctgta gcctaatgcc agcaattttt ccgccagcca cggattacga
    39541 ccaaattgct tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatgagc
    39601 cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg ggcgattttg
    39661 ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
    39721 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
    39781 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagccatcc
    39841 ataatccata aagattggcc agaaaatagc cttcttcaca cgggttaact tccagagcga
    39901 attccttcaa caactcctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
    39961 gttccgccag caactcgtta tccagcggct ctatttcttc accacggcga ctgcgcatca
    40021 gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggcagatggc
    40081 actctttttc cagtacgttg acgacctgca tcatgccttt gcagacgtcc ttatcaataa
    40141 cgcctgcctc gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa
    40201 ctgggttcct gttccccact caccacccgt tgttgatggg caagcagggc gtgtttatct
    40261 acaacagcac gcagcaattg ttgcattggg taatcatcga ctcgcgtcag gaccacggct
    40321 tcggtggcct gtaaaaatcg cggatcgtct gttagcggcg tcgcctctaa tcccagcatg
    40381 gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc tttaacaatg
    40441 cgttgtaaac tctcgtgata agagagatcg actcgttcca catcactatc gccaaaaaag
    40501 acatcggtca tgattttctg atccgccgaa cgactatcga gacctactcg cttaacattt
    40561 ccagactcac ctttgcggca tatcagttga tgttcaccaa catacgtatg cgggccaagc
    40621 tcgagggcga tgcacaaatt gttttggctg agataacttt ccgccgccag gcgggaaact
    40681 actgccatgt catagacacc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
    40741 tgcgcatagt aaaaaggaat gccatcaaac tgggctttca atccgctcgc taaaccttcg
    40801 tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc cacatgactc
    40861 agcaatgctt tgttatccat ctcgacaaga taactgccat tgcgcccacg gcgttcaatc
    40921 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aaccgatgaa
    40981 cggcattcag ttgccagctc atctatggtt ttcagtcgat taccgcattt ttcacccaac
    41041 aaataacggg ccagtgtcgt gatgacgacg ccttcttttt tgataaacgt tcgacgcata
    41101 atatattttc aataaaatga atatttatat cttcattaaa atgaagataa gtggcgacga
    41161 cgagatgtgg aaacggcgag gcgcttcaca ttttttcggg ttgttgcgaa aagaggataa
    41221 aaaaaccggg tttccccggt ttcagagtga tgacgaagtg atttattcga caatgcgagc
    41281 aaagggtaat tcccactggc gcttgcctgc tcccctcacc ctaaccctct ccccaaaggg
    41341 gcgaggggac cgtccgagcg ttttgatatt acggcttcgc cacaaaacca atcgcttcgt
    41401 acaccgcttt cagcgtacgg gaagcgtgta cactggcttt ttctgcgccg tctttcatca
    41461 cctgttgcag gaaggcttca tcgttgcgga aacggtgata gcgttcctgc aattcagtca
    41521 gcataccgga aacggcatca gccacttcac ctttcagatg accatacatc ttgccttcga
    41581 actgtttttc cagttctggg atgctctggc ccgttaccgc agaaaggata tccagcaggt
    41641 tggaaacgcc cgctttgttc tgcacatcgt agcgaactac cggcggctcg tcggagtcag
    41701 tgaccgcgcg tttgattttc ttcactaccg atttcggatc ttccagcagg ccgataacgt
    41761 tattgcggtt atcgtcagac ttggacatct tcttggtcgg ctccagcagc gacattacgc
    41821 gcgcgccaga tttcggaata aacggctccg gcaccttaaa gatctcgcca tacagcgcgt
    41881 tgaaacgctg ggcgatatcg cgactcagtt ccaggtgctg tttctggtct tcacccaccg
    41941 gtaccagatt agtttgatac agcaggatgt ccgcagccat cagcaccgga tagccaaaca
    42001 gaccagcgtt gatgttctcg gcataacgcg cagatttatc tttaaactgc gtcatgcgac
    42061 tcagttcgcc gaagtaggta tagcagttca gtgcccagcc taactgcgca tgttctggta
    42121 cgtgggactg aacaaaaatg gtgcttttct caggatcgat accacaagcc agatacaagg
    42181 ccagcgtatc caacgtcgct ttacgcagct tctgtgcatc ctggcgcacg gtgatcgcgt
    42241 gttggtcaac gatacagtaa atgcaatggt agtcatcctg catgtttacc cactgacgca
    42301 gcgcacccat gtagttacca atggtcaatt cacctgaggg ctgtgcgcca ctaaaaacga
    42361 tgggcttagt catttttcga ttcctgattt tcgctatgcg gaagccctaa tgcgggcaga
    42421 aggtcattta tagactgata aattacatca ggctggctga gatcgatagc ctcgccgtag
    42481 ttgtatccgt aggttaagcc aactgatggg caacctgccg cttttgccgc ctgaatatca
    42541 ttccgtgagt cgccgacaaa cagcatctgt tgtggggcaa ttcccatccg ctcagccacc
    42601 agtaacagcg ggtccggatg cggttttttg ttttgcacat catcgccgcc gataaccacg
    42661 ctaaagtatt tggcaatatc taaggcttcg agcagcggcg cgacgaacgg cgtcggtttg
    42721 ttggtgacca agcctagcgg caggccttta gcctgcaacg cgcccaacgt atcggcaacg
    42781 tgcgggaaca aaaacgtccc ctcttcggca acctcgccat agtagcgatc gaacagttta
    42841 cgcagaatac gtacctgttc ttctgccgga atgtcgtcat caacgggcgg tttccccatt
    42901 gttttacgca gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc catcagaaca
    42961 tctgcgccgt taccaatcca ggtaataacg cgttcttcac ctgcgacggg caactccagc
    43021 gcatacagcg ccatatctac cgcagcagca agaccaggag cactgtcgac cagcgtacca
    43081 tcaagatcaa aagcgacgcc gcgaatatct tcaaacttat tcatgactta cctttgccag
    43141 ttcactgcgc atttcatcaa tgactttttt atagtctggc tggtcgaaga ttgccgaacc
    43201 ggcgacgaac atatccgcgc ccgccgcagc gatttcgcca atgttgttca ccttcacgcc
    43261 accgtccact tccagacgaa tgtcaaagcc agactcgtcg atacggcgac gtacttcgcg
    43321 cagtttatcc agtgtttgag gaatgaaaga ctgaccgccg aaaccagggt tgacggacat
    43381 cagcaggatc acatccagct tatccatcac gtaatccaga tagctcagag gtgtcgccgg
    43441 gttaaatacc agacccgctt tacagccatt ttctttaatc agttgcagcg tgcggtcaac
    43501 atgctcggag gcttctggat gaaaggtaat gatgctggca ccagcggcag cgaaatcagg
    43561 cacaatgcga tcgacgggtt tcaccatcag atgtacgtcg ataggggcgg taatgccgta
    43621 gttacgcaag gatttcagca ccattggccc aatcgtcaga ttgggaacat agtggttatc
    43681 catgacgtca aaatgcacga catcagcgcc agctgccagg gcttttgcgg tatcttcacc
    43741 caggcgggca aaatcagccg acagaattga gggggcaatc aaatactgtt tcatccgctt
    43801 ctccttgaga attatttttt cgcgggtgaa acgactcctg gtttgtacaa agccagcagt
    43861 tcgtccacct ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac tttgacgaca
    43921 tgcaattttg cgcgctgata ccactcacgc gttaacatcg tatcgtgatt ggagatcagc
    43981 actggaatat ggcgctcaac cagaccttcg gcgatctccg ccagatgcgc ttgttgttca
    44041 agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg tcgcagacag cggtgcataa
    44101 ggcggatcgc aatagacgac ggatgcatca tctgcgcgct ccatgctatc ggcgtaagac
    44161 tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc tgcttccggg
    44221 aaatagggtt ttttgtagcg gccaaacggc acgttaaact caccgcgcag attgtaacga
    44281 cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg acggaacgga
    44341 tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa cctcggcgca atttgtttcg
    44401 ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag tacgcatctt cacaatgtta
    44461 tagagactga tcaggtcgct attgatatcg gcaaggatat aacgagaaaa gtcggtgttg
    44521 agaaacaccg acccggcacc tacaaaaggc tcaaccagac attcgccctt gggcaaatgc
    44581 cgtttaatat catcaagcag gggatacttg ccccctgccc acttcaaaaa agcgcgattt
    44641 ttcttcatgc cgattaacta attacacctt ctccggctgt ggagaaagct ccgacagcat
    44701 ccagcgcttc aggcagctcc cgcaacgata aaccattgcg ggagataacc ttgattactt
    44761 cagatcggcc tgtacctgac gcagcggttt cgcccacggg tttttggcct gaacatctgc
    44821 tggcaatgta gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc cagaaaccag
    44881 gacataccac ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt tcagattctc
    44941 tttcttcgcc caaccgttca ggttgtcgta gttagaggaa ctgctcagct gcagagtgta
    45001 atggctggac ggtgccgatt tcaacgaacc aacattacct gcggtcttcc ctccagcggc
    45061 tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact ggagccgtag tcgccgtctc
    45121 ttttggtgct ggcgtagaag tcgcagccgg tgccttcgtg ctcgccacag gggcagctgg
    45181 ttcaggttca gtacgcttcg gcgtctgtgc taccggcttc ggctccgttt tcacggttgc
    45241 ttgcggtttt ttcggttcaa tcaccgcctg ctgacgaact gggcgcgtag tggacggacg
    45301 ttcagcggtt tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgaa caggcgcaac
    45361 cgttgcgggt tcagtcggca acgtggaatt gaccgccaca ttgttcagct gttgctgatt
    45421 ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc
    45481 cgttgccacc ggggtttgcc cttgagtcgg cgtagaagag atcggtggca gagaaacatc
    45541 ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat tcgcctgatc
    45601 ggtcgcattg ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaagt
    45661 ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat
    45721 gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt
    45781 acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc
    45841 ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc
    45901 agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc
    45961 agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag
    46021 ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac
    46081 gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt
    46141 acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa
    46201 cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct
    46261 tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg
    46321 gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc
    46381 cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta
    46441 accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca
    46501 gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac
    46561 aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat
    46621 tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg
    46681 ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca
    46741 ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag
    46801 ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac
    46861 gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc
    46921 caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg
    46981 tatcgagtac agctaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt
    47041 taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt
    47101 cgctggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat
    47161 taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc
    47221 tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca
    47281 gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc
    47341 atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa
    47401 cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg
    47461 ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc
    47521 ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat
    47581 accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc
    47641 gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc
    47701 ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc
    47761 ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact
    47821 aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga
    47881 aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg
    47941 gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc
    48001 taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc
    48061 cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat
    48121 cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcata
    48181 aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca
    48241 ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg
    48301 gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc
    48361 cgcccagcgc caacgtttct ccgcttttta cctcgacctg cgtttcgatc tcctgcttat
    48421 caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga
    48481 tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca
    48541 ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg
    48601 gaatttcgct ctcctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac
    48661 tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaagc
    48721 gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt
    48781 cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc
    48841 atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct
    48901 caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg
    48961 ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg
    49021 cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta
    49081 tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg
    49141 cgatattgtc attctgccag gcaatggaat gcactgagag aatgttgcct tcctgccgcg
    49201 ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta
    49261 gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt
    49321 cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt
    49381 gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct
    49441 tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggta
    49501 cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg
    49561 ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg
    49621 cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatatagat
    49681 cttccggcga tttaaaaggg tcacgcatac cggttaaaag gcacaatgca atacctgcca
    49741 acaaccagcg tttaaccctc atgcggcatc tccagttgta gcgtgaacaa aagatcatca
    49801 ccttccacgc ttaacgaaaa acggctcacg ctgacgttgc gctctgccag ccgtgtaaat
    49861 gccgacggca ctgcttccca cagcgttttc aacgccaact cacctccctg cgcggatgga
    49921 tgccaggaaa ccagttgcgc gccggataac tgaaaatcca gtggcgaaaa gggcagcgtt
    49981 ttttcctcgc tgaagggagt ggtgtctacc aggcgataca gtgcggccca ctgctggtga
    50041 tgacttgccc gtagtcgaat taatgcgtcg cgctcttcat ggtgtgtcga tgatagaaaa
    50101 atgagcgtaa ctaacatcag caaccagaat gcccagcaaa actggcggag gcggggtgat
    50161 gtggcgaacc accagtcaaa gaacatgttc atcgctaacc ttccttgtta actgatactc
    50221 aaattgccag cgtccctgcg catcctgctg cgtggctccc cgctgattga gatgaaaaga
    50281 cgcatcctgg cggagtgacg tttctagtgc gtttaacgcg gtaatgcttg ttgtcagccc
    50341 cttgatctcc agcgttccct gctgccagct tatcgttgtc agccaggcgt gctcgggtaa
    50401 aagcgccgcc agtgcttcca gcgcagattg ccagtcgcgg gtaaattgtc gctggcgctg
    50461 ccgttgcgaa cgctgttcgc gtaactgctg ttgctccagc aaacgtggct tcgttatctg
    50521 taagctgcgg gcgagttgtt gttccgcctg aagcaaaact gcgtctatgc gcgcttcggc
    50581 gctgcctgtc agacgcagta ttagcgttat cccgacggcc agcagcagag gcgcaacgaa
    50641 catcagcaac cagaaacgca gaaaagcggt ccggcgttgc tgtcgccagg gcaaaaaatt
    50701 aattggcgga ttcatcagta cgccttccca agcgccagcc ccagcgcgat ggcaaagtct
    50761 ccaccaggcg gcggtagcgg cggctgacga acagaaacgg cctcccaggg gtcaaatccg
    50821 ccttcgccac atatcgcgac gctgtcggga tcaacggata acgctgccgc cagctccttc
    50881 gcgctagtca tccccaccgc cagtttgcgc ccccagctat agcgcgtcgc ccacagccac
    50941 tgttcgttat cacgccaggc cagacattgc tgatgagaag gtaaaaaagg caggaatcgc
    51001 tgtaatgcac tggcatccgg ggtgatcgca ctaacatgaa cacgcaacct ttctgccagc
    51061 gtaagcagcg ttgccagctc tttgctttgc gcggcagtca cgttataagc ggggctgagt
    51121 gagtcttcgc tgtaatcgaa gcgcagggag tccggatcca tatccagctc gcgggccatc
    51181 gtccctgaca gccaggccgt ttgctcccgc tcaccaaggg acatcgacgg gcgcggaaat
    51241 gaccgctgta atgtgcgact ggcgggaaac gccaacataa tgtgatgacg ctgcggcagt
    51301 tcgcgactcc acggtaacaa cgttttagcc agctgctgcg catcaacaat ccgcccatct
    51361 ttgataatgt cgtgcgccag cggcaaccgc caccagcgtt gcaaaaagca ttcttttgcg
    51421 ccccgcacga tcgcaaccgc tgccgcttct tgctgttgta aatgcaaacc aatttgccag
    51481 atcttaaatg tcattgtgat gatctcctta tcacccgtca ctctgacggg tatatcaatg
    51541 cgtctggctt gcctttatac taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa
    51601 tgggaaattt ccagtgaagt tcgtaaagta ttttttgatc cttgcagtct gttgcattct
    51661 gctgggagca ggctcgattt atggcctata ccgctacatc gagccacaac tgccggatgt
    51721 ggtgacatta aaagatgttc gcctgcaaat tccgatgcag atttacagcg ccgatggcga
    51781 gctgattgct caatacggtg agaaacgtcg tattccggtt acgttggatc aaatcccacc
    51841 ggagatggtg aaagccttta tcgcgacgga agacagccgc ttctacgagc atcacggcgt
    51901 tgacccggtg gggatcttcc gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca
    51961 aggggcgagt accattaccc agcagctggc gagaaatttc ttcctcagtc cagaacgcac
    52021 gatgatgcgt aagattaagg aagtcttcct cgcgattcgc attgaacagt tgctgacgaa
    52081 agacgaaatc ctcgagcttt atctgaacaa gatttacctt ggttaccgcg cctatggtgt
    52141 cggtgctgcg gcacaagtct atttcggtaa aacggtcgac caactgacgc tgaacgaaat
    52201 ggcggtgata gccgggttgc cgaaagcgcc ttccaccttt aatccgctct actcaatgga
    52261 tcgtgccgtc gcgcggcgta acgtcgtact gtcgcggatg ctggatgaag ggtatatcac
    52321 ccagcaacag ttcgatcaga cacgcactga ggcgattaac gctaattatc acgcgccgga
    52381 gattgctttc tctgcgccgt acctgagcga aatggtgcgc caggagatgt ataaccgtta
    52441 tggcgaaagt gcctatgaag acggttatcg catttacacc accatcaccc gcaaagtgca
    52501 gcaggccgcg cagcaggcgg tacgtaataa cgtgctggac tacgacatgc gccacggcta
    52561 tcgcggcccg gcaaatgtgc tgtggaaagt gggcgagtcg gcgtgggata acaacaagat
    52621 taccgatacg ctgaaggcgc tgccgaccta tggtccgctg ctgcctgccg cagtcaccag
    52681 cgccaatcct cagggagcga cggcgatgct ggcggacggg tcgaccgtcg cattgagtat
    52741 ggaaggcgtt cgctgggcgc gtccttaccg ttcggatact cagcagggac cgacgccgcg
    52801 taaagtgacc gatgttctgc aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc
    52861 atggtggctg gcacaagtgc cggaagtgaa ctcggcgctg gtgtcgatca atccgcaaaa
    52921 tggtgccgtt atggcgctgg tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc
    52981 cacccaggca ctgcgtcagg tgggttccaa catcaaaccg ttcctctaca ccgcggcgat
    53041 ggataaaggt ctgacgctgg caagtatgtt gaacgatgtg ccaatttctc gctgggatgc
    53101 aggtgccggt tctgactggc agccgaagaa ctcaccaccg cagtatgctg gtccaattcg
    53161 cttacgtcag gggctgggtc agtcgaaaaa cgtggtgatg gtacgtgcaa tgcgggcgat
    53221 gggcgtcgac tacgctgcag aatatctgca acgcttcggc ttcccggcgc aaaacattgt
    53281 ccacaccgaa tcgctggcgc tgggttcagc gtccttcacc ccaatgcagg tggcgcgcgg
    53341 ctacgcggtc atggcgaacg gcggcttcct agtggacccg tggtttatca gcaaaattga
    53401 aaacgatcag ggcggcgtga ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga
    53461 tattccagtg atttacggtg atacgcagaa atcgaacgtg ctggaaaata acgatgttga
    53521 agatgtcgct atctcccgcg agcagcagaa tgtttctgta ccaatgccgc agctggagca
    53581 ggcaaatcag gcgttagtgg cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa
    53641 cactccgctg gcattcctga ttaagagtgc tttgaacacc aatatctttg gtgaaccagg
    53701 ctggcagggt accggctggc gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa
    53761 aaccggtacc actaacagtt cgaaagatgc gtggttctcg ggttacggtc cgggcgttgt
    53821 gacctcggtc tggattggct ttgatgatca ccgtcgtaat ctcggtcata caacggcttc
    53881 cggagcgatt aaagatcaga tctcaggtta cgaaggcggt gccaagagtg cccagcctgc
    53941 atgggacgct tatatgaaag ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc
    54001 tccgggtatt gtgacggtga atatcgatcg cagcaccggg cagttagcta atggtggcaa
    54061 cagccgcgaa gagtatttca tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt
    54121 gggaacgacc attatcgata atggtgaggc acaggaattg ttctgattaa aaaggcgctt
    54181 cggcgccttt tcagttggct gacaaagtgc atttgtttat gccggatgcg ggtgaacgcg
    54241 ttatccggct aacaaaatcg tgaaaaatca atatattgca ggaaccccat aagcctgata
    54301 agcgttgtgc atcaggcaaa cttcacgcat ttacactcgc ccctgccctt tcaaccattc
    54361 gcgcacgagg aacagcgcgc tgacattacg cgcttcgttg aagtcagggt cttccagcaa
    54421 atccatcata tgcgccagcg gccagcgcac ctgtggtagc ggctctggct catcgccttc
    54481 cagcgattcc gggtagagat cttgcgcgac cacgatattc attttgctgg aaaagtaaga
    54541 cggtgccatg ctgagcttct tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc
    54601 ttttagctcg cggttagcgg cttcgtagac gctttcaccc ggatcaatta atcctttcga
    54661 aaaacctaat tcgtaggatt cagttcccac tgcgtattcg cggatcagga tcaggtgatc
    54721 gtccacaatc ggcacaatca tcactgcttc ccggttggtt ggacgcattc gttcataaac
    54781 acgccgcacg ccattgctga actccagatc cacgctctcg acggtaaaca gtcgggaacg
    54841 ggctacagtt tcaacattca gaatggtggg tttttgtaat gatttgttca tcgtgggatc
    54901 tatgctgtga aatcagcggt tattgtgcga tatcggacac gctttcggca atgtgaattg
    54961 catgttattt acatttatgt aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca
    55021 ataggttgaa ataactccag gaatttgctg atattccgcc ttcagagggt ttgctatgat
    55081 cagcggttac tgtgatgtgc ttaatgatgc tcaagttaag ctccacgctt gccgatagcc
    55141 aaccgcagaa tcatgtattg tgtccggtgc gactgaccac gcctgacaga ctaagtaaga
    55201 tggggaaagc atgagcacca ttgtgatttt tttagctgct ttgctggcct gctcactact
    55261 tgcgggatgg ctgataaaag tgcgatccag acggcgtcag ctgccctgga ccaacgcctt
    55321 cgcggatgcg caaacgcgta aactcacacc tgaagaacgt agcgccgttg aaaattatct
    55381 tgagagcctg acgcaggtat tacaggtgcc tggcccaacg gaagctagcg cggcaccgat
    55441 ctctctggcg ctgaatgccg aaagcaacaa cgtcatgatg ctgacacacg ctatcacacg
    55501 ttacggcatc tctaccgacg atccgaataa atggcgttac tacctcgatt cggtagaagt
    55561 ccacctgccc cccttctggg aacagtacat caacgatgag aataccgttg aactgattca
    55621 taccgattcg ctgccgttgg ttatttcact caacggtcat acgctgcagg agtacatgca
    55681 ggaaacgcgc ggctatgcct tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga
    55741 agagagtgag caaatcgagc tactcaatat tcgcaaagaa acgcatgaag aatatgcgct
    55801 gagtcgtccg cgcgggctgc gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt
    55861 tttctgcctg attaccccgg atgtatttgt tccgtggctg gcaggcggcg cattactgct
    55921 gctgggcgca ggtctgtggg ggctattcgc gcccccggca aaatcctccc tgcgagaaat
    55981 tcattgtctg cgcggtacac cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca
    56041 gatcaacaat atttcactcg gtattatcga cctggtctat cccgcacact ggcagccata
    56101 cattgctcag gatctcggtc aacaaaccga tattgatatc tatctcgacc gccacgtagt
    56161 gcgtcagggg cgatatcttt cgctgcatga tgaagtaaaa aactttccgt tacagcactg
    56221 gctgcgcagt acgattatcg ctgcgggctc gctgctagtg ctgtttatgc tgttattctg
    56281 gatcccgctg gatatgccgc tgaaattcac tctctcatgg atgaaaggcg cacagaccat
    56341 tgaagccacc agcgtaaaac aactggctga cgctggcgtg cgggtaggcg atacattgcg
    56401 tattagcggt acgggaatgt gtaatattcg cacttccgga acctggagcg cgaaaaccaa
    56461 ttcacctttt ttaccgtttg actgctcgca gatcatctgg aatgacgccc gctcattacc
    56521 attaccagaa tctgaactgg tcaacaaagc gacggcattg actgaagcag ttaatcgcca
    56581 gctgcaccct aaaccggaag atgaatctcg cgtcagtgcc tcattacgtt cagcgattca
    56641 aaaatccggc atggtattgc ttgatgattt tggcgacatt gtactgaaga cagcggattt
    56701 atgttctgcc aaagatgact gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag
    56761 taaagactgg gacgcgctgg taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt
    56821 gttattacgc ccggtgagtg ctgaatcgct ggataacctg gtggcaacct ccaccgcgcc
    56881 gtttatcacg catgaaacgg cgcgagcggc acaatcacta aacagtccgg cccccggcgg
    56941 attcctgatt gtcagcgacg aaggcagcga ttttgttgat cagccctggc cttcggcatc
    57001 actttacgac tacccgccgc aagaacagtg gaacgctttc cagaaactgg cacaaatgct
    57061 gatgcatacg ccgtttaacg ccgaaggtat cgtcacaaaa atcttcactg acgccaatgg
    57121 tacgcagcat attggccttc atccgatccc ggatcgttcc ggcctgtggc gctatctcag
    57181 caccacattg ctgctactga cgatgctggg tagcgccatt tacaatggcg tacaggcctg
    57241 gcgtcgttac cagcgtcatc gcactcgcat gatggagatt caggcctatt atgaaagctg
    57301 cctgaacccg caactgatca ccccttcaga aagccttatc gaataacacg tttgcgcggc
    57361 aggttatgct accctgtcgc gcaaattgct tcactctgga gatttccctc atgcatatca
    57421 acattgcctg gcaggacgta gataccgttc tgctggatat ggacggcacg ttgctcgacc
    57481 tcgccttcga taactatttc tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg
    57541 gggttacgcc acaggaagcg atggaatata tgcgccagca atatcacgac gtacagcata
    57601 cgctaaactg gtactgtctt gattactgga gtgagcaact gggtctggat atctgtgcga
    57661 tgaccaccga gatgggaccg cgcgccgtac tgcgtgaaga taccattccg tttcttgagg
    57721 cactgaaagc cagcggtaag cagcgaattt tgctcaccaa tgctcatccg cacaacctgg
    57781 cggtaaaact tgagcatacg ggtctggacg cacaccttga tttattactt tccacccaca
    57841 catttggtta tccgaaagag gatcagcggt tatggcatgc ggtggccgaa gctacgggtc
    57901 tgaaagctga aagaacgctg tttattgatg acagcgaagc gattctcgat gctgccgcgc
    57961 aatttggtat tcgttactgc ctcggcgtga ctaatcctga ttccgggatt gccgagaaac
    58021 agtatcaacg ccatccgtca ctgaatgact accgccgcct gatcccctcg ctaatgtgaa
    58081 ggagacgcca tgaaagagaa acctgctgtt gaggttcgac tggataaatg gctatgggct
    58141 gcccgttttt ataaaacccg cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat
    58201 tacaacgggc agcgcagcaa gccgagcaaa atcgtcgagc tgaatgccac gctcactctg
    58261 cgccagggaa atgacgaacg cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc
    58321 gccagcgagg cagccttgct gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa
    58381 atggcgatgg cacgtaaact taatgcctta accatgccgc acccggaccg acgcccggac
    58441 aaaaaagagc gccgcgacct gttacgattt aaacacggcg acagtgaata actgtcacct
    58501 gcaagagaga tgattatgcc gcaacatgac caattacatc gctatctgtt tgaaaatttt
    58561 gccgttcgcg gcgaactggt aaccgtttcg gaaaccctgc aacagatcct tgagaaccac
    58621 gattatccgc agcccgttaa aaacgtgctg gcagaactgc tggttgcgac cagcctgtta
    58681 accgctacgc tgaagtttga tggtgatatc accgtacagc tgcagggcga cggtccgatg
    58741 aatctggcgg ttattaacgg taacaataac cagcaaatgc gcggtgtggc gcgcgtgcag
    58801 ggcgaaattc cagaaaatgc cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc
    58861 accattaccc cgagcgaagg cgaacgctat cagggcgtag ttggtctgga aggtgatacc
    58921 ctggcggcct gcctagaaga ttactttatg cgttctgaac agctgccgac gcgcctgttt
    58981 attcgcaccg gcgacgtaga cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg
    59041 cctgcgcaaa atacccaaca ggacgacttt gaccacctgg cgacgctaac cgaaaccatc
    59101 aaaaccgaag aactgctgac cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa
    59161 gaagaggtga cggtttacga tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa
    59221 cgttgcgccg atgcgctgaa aacgctgcct gatgaagaag ttgatagcat cctggcggaa
    59281 gatggcgaaa ttgacatgca ttgtgattac tgcggtaacc actatctgtt caatgcgatg
    59341 gatattgctg aaatccgcaa caacgcgtct ccggcagatc cgtaagttca ttaatgagta
    59401 tgtccggcag agagggctct gccggatttt acgacttaat ggcctgatgt gctgcgctta
    59461 ccagcgccta tgaaataaat cgcaaattat tttggtcaga tacagcgtcg tatccgataa
    59521 aagcgttgtc acttacttaa gtttatttcc agcgtttcac catctaccgt cagatcgcgc
    59581 cacgctttcg gcctcgtccc cagttttcca tccttcgcag ctgtgagtaa tttttctttc
    59641 gttatttcgt tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca
    59701 aagccataga caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca
    59761 tcactattca gatcttgctc gaccagtacg caggcgtctt tttcaataca ggtcatcacg
    59821 ttatagcgac cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccaggg
    59881 gcaattaaca cattttcggc gaatactttt tccgatacct gttcctgaag atgttgctct
    59941 ccatctaacg ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg
    60001 cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata
    60061 gttacctggt ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta
    60121 atacgcatac tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac
    60181 gaaaccgcaa ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg
    60241 atactgacaa aataccccag cgaccacacc agtaacacca gctccgccag cgcgccctgc
    60301 aagcggtcga cagtccagcc gtactgagcg acccgcagcc ataaccccca ggtggcgacg
    60361 aacacataca gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc
    60421 cagggaagtg acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag
    60481 gccagcgtta acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggcccgta
    60541 aacggcaggg tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg
    60601 gcgattagcg tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg
    60661 atcaccgcca gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa
    60721 aagagcgtat tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc
    60781 accagccacg tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat
    60841 actgactggt aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt
    60901 atccacggta gcaacaacat tgccatcaac agcagatagc agccaaaatc ccaaatcgcc
    60961 ttttcagctc tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct
    61021 gtcgtggcaa taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc
    61081 acggagaaga gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa
    61141 ctgtgatttt ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat
    61201 ccggtagcaa tcatcccctg aagtaatccg gtagcaatca tcccccagcg tgttgccggt
    61261 gaaagttcaa cgttatccat gttggttatc cagaatcaaa aggtgggtta attatcgcat
    61321 ccgggcagta gtattttgct tttttcagaa aataatcaaa aaaagttagc gtggtgaatc
    61381 gatactttac cggttgaatt tgcatcaatt tcattcagga atgcgattcc actcacaata
    61441 ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc taaaatgcta
    61501 tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg tcaaatatga
    61561 atttctccag atacgtaaat ctatgagcct tgtcgcggtt aacaccccca aaaagacttt
    61621 actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac aatggtttgc
    61681 ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt tacaacccaa
    61741 gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat gagcgcgggg
    61801 tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt cgttcaccaa
    61861 aagataagta tatcgtccgt gacgatacca ctcgcgatac tttctggtgg gcagacaaag
    61921 gcaaaggtaa gaacgacaac aaacctctct ctccggaaac ctggcagcat ctgaaaggcc
    61981 tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga
    62041 acccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag gcgcattttg
    62101 tcaaaaacat gtttattcgc ccgagcgatg aagagctggc aggtttcaaa ccagacttta
    62161 tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaacagggt ctgaactccg
    62221 aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct gattggcggc acctggtacg
    62281 gcggcgaaat gaagaaaggg atgttctcga tgatgaacta cctgctgccg ctgaaaggta
    62341 tcgcttctat gcactgctcc gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg
    62401 gcctttccgg caccggtaaa accacccttt ccaccgaccc gaaacgccgc ctgattggcg
    62461 atgacgaaca cggctgggac gatgacggcg tgtttaattt cgaaggcggc tgctacgcaa
    62521 aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg
    62581 cgctgctgga aaacgtcacc gtgcgtgaag atggcactat cgactttgat gatggttcaa
    62641 aaaccgagaa cacccgcgtt tcttatccaa tctatcacat cgataacatc gttaagccgg
    62701 tttccaaagc gggccacgcg actaaggtta tcttcctgac tgctgatgct ttcggcgtgt
    62761 tgccgccggt ttctcgcctg actgccgatc aaacccagta tcacttcctc tctggcttca
    62821 ccgccaaact ggccggtact gagcgtggca tcaccgaacc gacgccaacc ttctccgctt
    62881 gcttcggcgc ggcgttcctg tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac
    62941 gtatgcaggc ggcgggcgcg caggcttatc tggttaacac tggctggaac ggcactggca
    63001 aacgtatctc gattaaagat acccgcgcca ttatcgacgc catcctcaac ggttcgctgg
    63061 ataatgcaga aaccttcact ctgccgatgt ttaacctggc gatcccaacc gaactgccgg
    63121 gcgtagacac gaagattctc gatccgcgta acacctacgc ttctccggaa cagtggcagg
    63181 aaaaagccga aaccctggcg aaactgttta tcgacaactt cgataaatac accgacaccc
    63241 ctgcgggtgc cgcgctggta gcggctggtc cgaaactgta atgatttgaa gctggagaat
    63301 atctatccag tatcttatag aaagcaaaac gggaggcacc ttcgcctccc gtttatttac
    63361 ccttcttttg tcgtgccctg cgcccgcgtt accggcactg gcagccaggc gcgaatggaa
    63421 agcccgcccc gctcgctggt gccaagctcc agcatcccgt tatggttatc cacgatacgc
    63481 tgcacaattg ccagccctaa tcccgtgccg ctaatggtgc gcgcactgtc gccgcggaca
    63541 aacggctgga acaggtgctt acgttgttcc ggcgcaattc ccggaccgtc atcttccacc
    63601 tggaaccagg cgcgattcgg ctccgttccg ctgctgactt tgatccagcc attgccataa
    63661 cgggcggcgt tgaccaccat attcgccacc gcgcgtttga tcgacagcgg gtgcattttc
    63721 acttcaatgc tgccggggta aagcgcggtt tcaatttccc gctcatagcc actttcggca
    63781 gcaatcacct caccgagcac tgcattaaga tccgccattt ccatcggcat ctcctgcccg
    63841 gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc actcttcgat atctttattg
    63901 atcgattctg ccagatagcc atcctgctcg ctcatcatct cagtcgccag gcgaatacgc
    63961 gtcagcggcg tgcgcaagtc gtgacttacc cccgccatca gcagcgtgcg gtcatccgcc
    64021 agttgcttaa caccagccgc catatggtta aaggcacggg taacggaacg cacctccgaa
    64081 gcgccatatt cacgcagcgg cggcggaata atccctttac caacctgcaa ggctgcgtgt
    64141 tcgagatcga ccaacggtcg gttctggata cgaataaaca gccacgcccc gcctatcgcc
    64201 aatagcataa tcgccagcgt atagcggaac agcggagaga aatcgccctg atgaatttcg
    64261 gtcagcggca cgcgtaccca gatattgggc gacagccagg ttttcagcca gacgacaggc
    64321 gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc ccagttgctg tgccatctga
    64381 tggcttaaga attcatagtg ttgcgcccaa cgcagacctg cctcttcggc agcctcgttg
    64441 gagtagagag agatccccag ctcacggtag atctcccgac ggaaagcggg aggcacaacc
    64501 aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg
    64561 aggactttat taaactgctg gaggctcggc aaaatcgcga agttcagcac caccagataa
    64621 gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa
    64681 cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc gtccggtaca aagacgtagc
    64741 ccaggcccca aacggtctga atgtaacgcg gatgcgctgg atcttcttct accatgcggc
    64801 gcagacgcga aatctgcacg tcgatggagc gttccattgc ggaatattca cgaccacggg
    64861 caaggttcat cagcttatcg cgggagagcg gctcacgcgg atggctgacc agtgccttca
    64921 gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg
    64981 taccgaggtt aagtttgaac ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc
    65041 ctggcagttc gttcgcctga cgacgcagca ccgcacgaat acgggccagc agttcacgcg
    65101 ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc caggcctacg atacggtcca
    65161 cttcttcccc tttcgccgtc accataatga tcggcatcgg gttgctctga ctacgaagac
    65221 gtcggcaaat cgacaagcca tcttcaccag gtaacattaa atccagtacc ataagatgga
    65281 aagattcacg agtcagcagg cgatccatct gttctgcatt agcgacgctt cgaacctgga
    65341 agccttgttc ggtgagataa cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca
    65401 ccagaatctt gtagttctct tgcattgttt gtactcccaa aggttcgcaa caatttgtaa
    65461 gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa atctggtatg aatttcagcc
    65521 taaattgtta caaagcatat taaacagcag cttaagtata caatttattc ggcgaaacat
    65581 tattgattct gttgatatga tcacgttata cccaatgtgc gcattatcaa acagacaaag
    65641 ggaatcaacg agatgaaaac gcccctggtt acccgggaag ggtatgaaaa actcaaacaa
    65701 gagcttaatt atctctggcg tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc
    65761 gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt
    65821 gaaatcgacc gtcgcgtgcg ctatctcact aaatgcctgg aaaatctcaa aatcgtcgat
    65881 tactcccctc agcaggaagg caaagtcttt tttggcgcgt gggtggagat tgaaaacgac
    65941 gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat
    66001 tacatctcta tcgattcccc gatggcccgc gcattgctga aaaaagaagt cggcgatctg
    66061 gcggtggtga atacccctgc cggggaagcg aactggtatg ttaatgctat cgagtacgtg
    66121 aaaccgtaag gaagagtctt aacctcctgc cgatggctgg catttttgcc agccagtccg
    66181 tataactatc ccctgatttt tgatccgaaa agatgaactc aaaccatgat gaatgattcg
    66241 ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt
    66301 cgcctgcttg acgaagggaa taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc
    66361 ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag
    66421 ctggaagaga gacgtcaggc gatcctcaaa tccatttccg agcaaggcaa actcaccgat
    66481 gatctggcga aggccatcaa cgccacccta agcaaaaccg aactcgaaga cctctacctg
    66541 ccctacaaac ctaaacgccg cacccgcggg caaatcgcca ttgaagcagg gcttgagccg
    66601 ttggctgacc tgctgtggag cgatccgtca cacacgccag aagtcgccgc tgcacaatat
    66661 gttgatgccg ataaaggcgt ggcagatacc aaagccgcgc tggatggcgc gcgctatatc
    66721 ctgatggaac ggtttgccga agatgccacg ctgctggcga aagtgcgtga ttatctgtgg
    66781 aagaacgcgc atttggtttc tacggtggtg agcggtaaag aagaggaagg ggcgaaattc
    66841 cgcgactatt tcgatcatca cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg
    66901 atgttccgtg ggcgtaacga aggcgtactc cagctttcgc tgaatgccga tccgcagttc
    66961 gatgagccgc ccaaagagag ctattgcgag caaatcatca tggatcacct tggcctgcgc
    67021 ctgaacaatg ccccggcgga tagctggcgc aaaagcgtag tgagctggac ctggcgcatc
    67081 aaggtgctga tgcatctgga aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat
    67141 gaagcaatca acgtctttgc ccgtaacctg cacgatctgc tgatggcggc ccctgccgga
    67201 ctgcgtgcaa cgatgggcct cgatccgggt ctgcgtaccg gggtaaaagt ggcggtggtc
    67261 gatgccactg gcaaactggt ggcgaccgat accatttacc cgcacaccgg acaagccgca
    67321 aaagcagcga tgaccgttgc tgccttgtgt gaaaaacata acgttgaact ggtggcgatc
    67381 ggcaacggta cagcttcccg cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc
    67441 ccgaaagtga ccgcacagaa agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct
    67501 tccgagctgg cagcgcagga gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct
    67561 atcgcccgcc gtttgcagga tccgctggcg gagctggtga aaatcgatcc gaaatctatc
    67621 ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac tggcccgcaa actggatgca
    67681 gtagtagaag actgcgtaaa cgccgttggc gtcgatctca acaccgcttc tgttccgcta
    67741 ttaacccgcg tggcgggcct gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat
    67801 gagaacggcc agttccagaa ccgtcagcaa ctgctgaaag tcagccgtct ggggccgaaa
    67861 gccttcgagc agtgcgcggg cttcctgcgc attaaccacg gtgataaccc gctggacgcc
    67921 tctaccgttc atccggaagc ctatccggtg gtggaacgca ttctggcagc aacacagcag
    67981 gcactgaaag atctgatggg taacagcagc gaactgcgta acctgaaagc gtctgacttt
    68041 actgatgaaa aattcggtgt gccgacagta actgacatca tcaaagagct ggaaaaaccg
    68101 ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg ccgatggcgt cgagacaatg
    68161 aacgacctgc aaccgggtat gatcctcgaa ggcgcagtga ccaacgtcac caactttggc
    68221 gcgtttgtcg atattggcgt gcatcaggac ggcctggttc acatctcttc attgtcgaac
    68281 aagtttgtgg aagatccgca taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg
    68341 ctggaagtgg atcttcagcg taaacgtatc gccctgacta tgcgtctgga cgagcagcct
    68401 ggcgaaacca actctcgtcg cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca
    68461 gccaaaccac gcggtcgtga agcgcagcct gccggtaata gcgcgatgat ggatgcgctg
    68521 gcggcagcga tgggtaaaaa acgttaaacg cccggactgg cccctaaacc tgcgcgataa
    68581 ggcgttcacg ccgcatctgg caacgaacgc tttgcctgac ataaaagtgc cggagaatat
    68641 ctccggcact tttattccac agccaaactc acaatatact ccgttaatat ttatcacttc
    68701 attaacaact gaaaccttaa ttaaacatta gccagtccgg gtaactcact attcgaatta
    68761 tattttcgct gcgatataac cttgagccac atcaacattg agtcagatta ttattcaaac
    68821 caacattcgc acacatttta agtattgctg atagaaacca ttctcattat cattgtgttg
    68881 ttgattattt aatctctcct ttgttggcaa atcatctggt ctcatgtcgc ggtcaaacgc
    68941 cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa tacactccag
    69001 atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat cgccaaaaac
    69061 tgctttctct aggcatgtta cctggctcct cttttgatgt ggtgcgcgtc gctccactcg
    69121 gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa aaagatctgg
    69181 ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat gaaaaaatta
    69241 accattggct taattggtaa tccaaattct ggcaagacaa ccttatttaa ccagctcact
    69301 ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa agaagggcaa
    69361 ttctccacca ccgatcatca ggtcacgctg gtggacctgc ccggcaccta ttctctgacc
    69421 accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactacat tttgagtggc
    69481 gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg
    69541 acgctgcaac tgctggaact cggcattccc tgcatcgtgg cgctgaacat gctcgacatt
    69601 gccgagaagc aaaatattcg tattgaaatt gatgctctat cggcacgtct tggctgcccg
    69661 gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat
    69721 cgctataaag ctaacgagaa tgtggaactg gtgcattacg cacagccgct actcaacgaa
    69781 gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg tcgctggctg
    69841 ggcctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga agcgtcgcag
    69901 catctggatg ccaccctcgc ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt
    69961 gccgatgcgc gttaccagtg cattgctgcc atctgtgatg tggtaagcaa caccctgacg
    70021 gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg tttcctcggt
    70081 ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa catcggcggg
    70141 gcgttacagc cgctgtttaa cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg
    70201 attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca gggcctgggt
    70261 ggcggtatta acaccgtgct gccactggtg ccgcagatcg gcatgatgta cctgttcctc
    70321 tccttccttg aggactccgg ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg
    70381 caggcgctgg gcttgccggg gaaatccttt gtgccgctga tcgtcggttt cggttgtaac
    70441 gtaccgtcgg taatgggcgc acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc
    70501 atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct
    70561 gccttcttcg ggcagaacgg tgccctggcg gttttctcgc tgtatatgct cggtatcgtg
    70621 atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga agcgacgccg
    70681 tttgtcatgg agctgccggt ctaccatgta ccacacgtta aaagcctgat tatccagacc
    70741 tggcagcgtc tgaagggctt cgttctgcgt gctggtaaag tgattatcat cgtcagcatt
    70801 ttcctgagcg ctttcaacag cttctcgttg agcgggaaaa tcgtcgataa catcaacgac
    70861 tcggcgctgg cttccgtcag ccgggtgata acccctgtct tcaagccgat tggcgtacat
    70921 gaagataact ggcaggcaac ggttggcctg tttacgggcg caatggcaaa agaagtggtg
    70981 gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga gttcaatccg
    71041 gcggaattca acctcggtga agagctgttc agtgcggtag atgaaacctg gcagagcctg
    71101 aaagacacct tcagccttag cgtactgatg aaccccattg aagctagcaa aggcaacggt
    71161 gaaatgggta ccggggcgat gggcgtgatg gatcagaaat tcggtagcgc agctgccgca
    71221 tacagctacc tgattttcgt cctgctgtat gtaccatgta tctcggtgat gggagccatc
    71281 gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct gaatatcgct
    71341 tactcactgg caacattgtt ctatcaggtc gccagctaca gtcagcaccc aacttacagc
    71401 ctggtgtgca ttctggcggt aatcctgttt aacatcgtag ctatcggtct gctgcgccgc
    71461 gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag cagttgctgc
    71521 gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggta cgtgatttgc
    71581 tggcgttacg gggccgtatg gaagcgaccc agataagcca gacattgaac actccgcagc
    71641 caatgattaa cgccatgctg caacagctgg aaagcatggg caaagccgtg cggattcagg
    71701 aagaacctga cggctgcctc tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc
    71761 tgcgcgagtg gtgggcgctg cgttaaccat actccatcgc ctgttttgaa aagcccggta
    71821 tgcgtttgca tccgggcttt tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg
    71881 caacgtaagt tcctctgaaa gcgtctcgca gggatgaaaa ctcgctaatg cacagatgtg
    71941 gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag
    72001 cagagttcca ccccacatga tgcgctgttc aaactctttt tacgccaacc ggagacggca
    72061 cgcgattttc ttgcgtttca tttaccggca cccattcacg cgctctgtga tatgaaaacc
    72121 ctcaagctgg agtcgaggag ctttattgat gacgatctgc gtgaaagcta ttccgatgtg
    72181 ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat tgaacatcaa
    72241 agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa
    72301 aatcaccttg atgctggata caaaacgttg ccgatggtgg tgccattgtt gttttaccac
    72361 ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc cgatcccaaa
    72421 ctggcaaggc agctttatgc ctccgcattt ccgctgattg atgtcaccgt catgcctgat
    72481 gatgaaatca tgcagcaccg acgtatggcg ctgctggagt taattcaaaa acatattcgt
    72541 caacgcgatc tgatggggct ggtagagcaa atggcctgct tattaagtag tggatacgct
    72601 aatgacagac aaatcaaagg gctgtttaat tacatactgc aaactggcga cgctgtacgt
    72661 tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga aacacaagga gagtttaatg
    72721 actattgcgg aaagattgcg gcaggagggg gaacaatcca aagccctgca tatagccaaa
    72781 ataatgcttg aatccggagt ccctcttgca gacatcatgc gatttaccgg gctgtcagaa
    72841 gaagag
//
LOCUS       DAJTYL010000003        71356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYL010000003 DAJTYL010000000
VERSION     DAJTYL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.003"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(849..2435)
                     /locus_tag="OO572_000180"
     CDS             complement(849..2435)
                     /locus_tag="OO572_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6659315.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2790..4961)
                     /gene="glcB"
                     /locus_tag="OO572_000181"
     CDS             complement(2790..4961)
                     /gene="glcB"
                     /locus_tag="OO572_000181"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6659316.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4983..5387)
                     /locus_tag="OO572_000182"
     CDS             complement(4983..5387)
                     /locus_tag="OO572_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6659317.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5392..6611)
                     /gene="glcF"
                     /locus_tag="OO572_000183"
                     /pseudo
     CDS             complement(5392..6611)
                     /gene="glcF"
                     /locus_tag="OO572_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6622..7674)
                     /gene="glcE"
                     /locus_tag="OO572_000184"
     CDS             complement(6622..7674)
                     /gene="glcE"
                     /locus_tag="OO572_000184"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6659318.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7674..9173)
                     /gene="glcD"
                     /locus_tag="OO572_000185"
                     /pseudo
     CDS             complement(7674..9173)
                     /gene="glcD"
                     /locus_tag="OO572_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9424..10194
                     /gene="glcC"
                     /locus_tag="OO572_000186"
     CDS             9424..10194
                     /gene="glcC"
                     /locus_tag="OO572_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6659319.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10318..11459)
                     /locus_tag="OO572_000187"
                     /pseudo
     CDS             complement(10318..11459)
                     /locus_tag="OO572_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11817..13547
                     /locus_tag="OO572_000188"
     CDS             11817..13547
                     /locus_tag="OO572_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6659320.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13544..14458
                     /locus_tag="OO572_000189"
     CDS             13544..14458
                     /locus_tag="OO572_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6659321.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14491..14739
                     /locus_tag="OO572_000190"
     CDS             14491..14739
                     /locus_tag="OO572_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6659322.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14739..15910
                     /locus_tag="OO572_000191"
                     /pseudo
     CDS             14739..15910
                     /locus_tag="OO572_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15946..17025)
                     /gene="lptG"
                     /locus_tag="OO572_000192"
     CDS             complement(15946..17025)
                     /gene="lptG"
                     /locus_tag="OO572_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6659323.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17022..18092)
                     /gene="lptF"
                     /locus_tag="OO572_000193"
     CDS             complement(17022..18092)
                     /gene="lptF"
                     /locus_tag="OO572_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6659324.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18123..18683)
                     /locus_tag="OO572_000194"
     CDS             complement(18123..18683)
                     /locus_tag="OO572_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6659325.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18695..19519)
                     /locus_tag="OO572_000195"
     CDS             complement(18695..19519)
                     /locus_tag="OO572_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659326.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19519..20868)
                     /locus_tag="OO572_000196"
     CDS             complement(19519..20868)
                     /locus_tag="OO572_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6659327.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20914..21672)
                     /locus_tag="OO572_000197"
     CDS             complement(20914..21672)
                     /locus_tag="OO572_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659328.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21704..22417)
                     /locus_tag="OO572_000198"
     CDS             complement(21704..22417)
                     /locus_tag="OO572_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6659329.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22591..23283
                     /locus_tag="OO572_000199"
     CDS             22591..23283
                     /locus_tag="OO572_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6659330.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23332..24831)
                     /gene="pitB"
                     /locus_tag="OO572_000200"
     CDS             complement(23332..24831)
                     /gene="pitB"
                     /locus_tag="OO572_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6659331.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25123..26982)
                     /gene="gss"
                     /locus_tag="OO572_000201"
     CDS             complement(25123..26982)
                     /gene="gss"
                     /locus_tag="OO572_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6659332.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27187..28053
                     /gene="yghU"
                     /locus_tag="OO572_000202"
     CDS             27187..28053
                     /gene="yghU"
                     /locus_tag="OO572_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6659333.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28176..28424)
                     /gene="hybG"
                     /locus_tag="OO572_000203"
     CDS             complement(28176..28424)
                     /gene="hybG"
                     /locus_tag="OO572_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6659334.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28558..28899)
                     /gene="hypA"
                     /locus_tag="OO572_000204"
     CDS             complement(28558..28899)
                     /gene="hypA"
                     /locus_tag="OO572_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6659335.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28892..29380)
                     /gene="hybE"
                     /locus_tag="OO572_000205"
     CDS             complement(28892..29380)
                     /gene="hybE"
                     /locus_tag="OO572_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6659336.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29373..29867)
                     /locus_tag="OO572_000206"
     CDS             complement(29373..29867)
                     /locus_tag="OO572_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6659337.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29867..31570)
                     /gene="hybC"
                     /locus_tag="OO572_000207"
     CDS             complement(29867..31570)
                     /gene="hybC"
                     /locus_tag="OO572_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6659338.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31567..32745)
                     /gene="hybB"
                     /locus_tag="OO572_000208"
     CDS             complement(31567..32745)
                     /gene="hybB"
                     /locus_tag="OO572_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6659339.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32735..33721)
                     /gene="hybA"
                     /locus_tag="OO572_000209"
     CDS             complement(32735..33721)
                     /gene="hybA"
                     /locus_tag="OO572_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6659340.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33724..34842)
                     /gene="hybO"
                     /locus_tag="OO572_000210"
     CDS             complement(33724..34842)
                     /gene="hybO"
                     /locus_tag="OO572_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6659341.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35031..35318)
                     /locus_tag="OO572_000211"
     CDS             complement(35031..35318)
                     /locus_tag="OO572_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6659342.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35437..36324)
                     /locus_tag="OO572_000212"
     CDS             complement(35437..36324)
                     /locus_tag="OO572_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6659343.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36481..37521
                     /gene="gpr"
                     /locus_tag="OO572_000213"
     CDS             36481..37521
                     /gene="gpr"
                     /locus_tag="OO572_000213"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6659344.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37561..38055)
                     /locus_tag="OO572_000214"
     CDS             complement(37561..38055)
                     /locus_tag="OO572_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6659345.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38246..39130
                     /gene="yghA"
                     /locus_tag="OO572_000215"
     CDS             38246..39130
                     /gene="yghA"
                     /locus_tag="OO572_000215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6659346.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39402..39827)
                     /gene="exbD"
                     /locus_tag="OO572_000216"
     CDS             complement(39402..39827)
                     /gene="exbD"
                     /locus_tag="OO572_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6659347.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39834..40568)
                     /gene="exbB"
                     /locus_tag="OO572_000217"
     CDS             complement(39834..40568)
                     /gene="exbB"
                     /locus_tag="OO572_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6659348.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40820..42007
                     /gene="metC"
                     /locus_tag="OO572_000218"
     CDS             40820..42007
                     /gene="metC"
                     /locus_tag="OO572_000218"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6659349.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42147..42806
                     /locus_tag="OO572_000219"
     CDS             42147..42806
                     /locus_tag="OO572_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6659350.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42846..43745)
                     /gene="yqhC"
                     /locus_tag="OO572_000220"
     CDS             complement(42846..43745)
                     /gene="yqhC"
                     /locus_tag="OO572_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6659351.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43939..45102
                     /gene="yqhD"
                     /locus_tag="OO572_000221"
     CDS             43939..45102
                     /gene="yqhD"
                     /locus_tag="OO572_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6659352.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45207..46034
                     /gene="dkgA"
                     /locus_tag="OO572_000222"
     CDS             45207..46034
                     /gene="dkgA"
                     /locus_tag="OO572_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6659353.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46234..47160
                     /locus_tag="OO572_000223"
     CDS             46234..47160
                     /locus_tag="OO572_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6659354.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47211..47468
                     /gene="yqhH"
                     /locus_tag="OO572_000224"
     CDS             47211..47468
                     /gene="yqhH"
                     /locus_tag="OO572_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6659355.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47511..49730)
                     /locus_tag="OO572_000225"
     CDS             complement(47511..49730)
                     /locus_tag="OO572_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6659356.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49841..51253)
                     /gene="ftsP"
                     /locus_tag="OO572_000226"
     CDS             complement(49841..51253)
                     /gene="ftsP"
                     /locus_tag="OO572_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6659357.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51328..52065)
                     /gene="plsC"
                     /locus_tag="OO572_000227"
                     /gene_synonym="parF"
     CDS             complement(51328..52065)
                     /gene="plsC"
                     /locus_tag="OO572_000227"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6659358.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52299..54557)
                     /gene="parC"
                     /locus_tag="OO572_000228"
     CDS             complement(52299..54557)
                     /gene="parC"
                     /locus_tag="OO572_000228"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6659359.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55103..55585)
                     /locus_tag="OO572_000229"
     CDS             complement(55103..55585)
                     /locus_tag="OO572_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6659360.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55638..56030)
                     /gene="ygiW"
                     /locus_tag="OO572_000230"
     CDS             complement(55638..56030)
                     /gene="ygiW"
                     /locus_tag="OO572_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6659361.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56182..56841
                     /gene="qseB"
                     /locus_tag="OO572_000231"
     CDS             56182..56841
                     /gene="qseB"
                     /locus_tag="OO572_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6659362.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56838..58112
                     /gene="qseC"
                     /locus_tag="OO572_000232"
     CDS             56838..58112
                     /gene="qseC"
                     /locus_tag="OO572_000232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6659363.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58158..58490)
                     /locus_tag="OO572_000233"
     CDS             complement(58158..58490)
                     /locus_tag="OO572_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6659364.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58809..59390
                     /gene="mdaB"
                     /locus_tag="OO572_000234"
     CDS             58809..59390
                     /gene="mdaB"
                     /locus_tag="OO572_000234"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6659365.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59421..59735
                     /locus_tag="OO572_000235"
     CDS             59421..59735
                     /locus_tag="OO572_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6659366.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59783..61675)
                     /gene="parE"
                     /locus_tag="OO572_000236"
     CDS             complement(59783..61675)
                     /gene="parE"
                     /locus_tag="OO572_000236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6659367.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61704..62285)
                     /gene="yqiA"
                     /locus_tag="OO572_000237"
     CDS             complement(61704..62285)
                     /gene="yqiA"
                     /locus_tag="OO572_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6659368.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62285..63112)
                     /gene="cpdA"
                     /locus_tag="OO572_000238"
     CDS             complement(62285..63112)
                     /gene="cpdA"
                     /locus_tag="OO572_000238"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6659369.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63137..63559)
                     /locus_tag="OO572_000239"
     CDS             complement(63137..63559)
                     /locus_tag="OO572_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6659370.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63560..64189)
                     /gene="nudF"
                     /locus_tag="OO572_000240"
     CDS             complement(63560..64189)
                     /gene="nudF"
                     /locus_tag="OO572_000240"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6659371.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64394..65875
                     /gene="tolC"
                     /locus_tag="OO572_000241"
     CDS             64394..65875
                     /gene="tolC"
                     /locus_tag="OO572_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6659372.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66023..66694
                     /locus_tag="OO572_000242"
     CDS             66023..66694
                     /locus_tag="OO572_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6659373.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66700..67860
                     /locus_tag="OO572_000243"
     CDS             66700..67860
                     /locus_tag="OO572_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6659374.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67898..68686)
                     /gene="ygiD"
                     /locus_tag="OO572_000244"
     CDS             complement(67898..68686)
                     /gene="ygiD"
                     /locus_tag="OO572_000244"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6659375.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68829..69602
                     /gene="zupT"
                     /locus_tag="OO572_000245"
     CDS             68829..69602
                     /gene="zupT"
                     /locus_tag="OO572_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6659376.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69660..69830)
                     /locus_tag="OO572_000246"
     CDS             complement(69660..69830)
                     /locus_tag="OO572_000246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659377.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70091..70744)
                     /gene="ribB"
                     /locus_tag="OO572_000247"
     CDS             complement(70091..70744)
                     /gene="ribB"
                     /locus_tag="OO572_000247"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6659378.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70848..70996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71118..>71356
                     /gene="ubiK"
                     /locus_tag="OO572_000248"
     CDS             71118..>71356
                     /gene="ubiK"
                     /locus_tag="OO572_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6659379.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENR"
BASE COUNT        17791 a        19025 c        17640 g        16900 t
ORIGIN      
        1 tttagcagga aatactcagg tgtcaacatt cgaactccaa ctctgaaata ttcatataag
       61 ttaatgattt taatgatttt aatgatttta atgatttttt catttattca cacctaataa
      121 aagtattcaa attcagatga tttttctgct caacagacac aaacaggcaa taatactctc
      181 caaagcgaac aatatttggc attacgattt aatgcttcat aatcaccaaa ttatttaacc
      241 ataaattttc ttatttctaa caaaatgatt ggtgtgttgt tggagttaaa atacatacat
      301 aatattaaca tctgccaaca tttcactttc cgcaagacgg atgatttcga atcctcatcc
      361 acgttaccct atcccaaata tctttattaa taggcagtaa cgaacactgt tatagcaaga
      421 ttaacttatt aacctaacgg catcttttat gtaacaagtg gagataaatt gcttaaaatt
      481 gatcctcgac aaatcttaaa agttctcatt attctgcaaa atcagctttg cgaataatct
      541 tatgtttttg ggagttttgg gaattttgat ctactcattg ttctagtgaa aaatataaat
      601 aagagaaaaa acgccattcg tcatgaaagc aacagatgaa gcaatgtaat aacatactaa
      661 aattgatctc atgaaagtgg gtactttata accagcacag aagccaagtt gtataaatat
      721 taaacaattc gcatcgtaaa acggagacta cgtataaaga agccaatcgt aaaaacggtt
      781 ccctggcgag ataaacgaca gacaaatgga aagccgggtg atatttacct tcatccggct
      841 ttattgattt acgagactaa catcccggta aacacatacg cctgcagcag ggtgataacg
      901 ctggcaaaaa tcagactgtg cttcacggtg tagcggaaca gttcagattc tcggcccacc
      961 atgcccgtcg cggcgcaggc cacggcgata gattgcgggg agatcatctt gccagttacg
     1021 ccgccgctgg tatttgctgc caccagcaga gtgtcagaga cgttgatttg ctgcgccgtg
     1081 gtcgattgca gtgaaccaaa cagggcgttt gaagaagtgt ccgacccggt aaggaatacg
     1141 cccagccagc cgagaaacgg tgagaaaaac gggaacatca cgcctgtacc tgccagtacc
     1201 agcgccagcg tggtggacat gccggaatag ttggtgacga aggcgaatgc cagcaccatg
     1261 ccaatcgaca atatcggcca cttcagacta attagcattt cggcaaagac gccaatccct
     1321 ttcttgatgc ccacgccgag gatgaagata gagataatcg ccgcaataaa aatagcggtg
     1381 ccgccagccg agagagggtc gaatttaaac accgcatcca ttggcgttgg ttgggcgaca
     1441 atgggtgccg ctttcaacac ttgttgatgc aaatgaggga tctggaaatt aatcaccagt
     1501 gaataaaacg cgccgcccgg agcaaataac gctttaaacg gcttcatggt ccagatggtg
     1561 accagcaccg ttaagattaa aaacggtgac cacgcacgaa tgatttgccc cagactatat
     1621 tctgaaggca cgggaccgcc agaagatggc ttatttacca ccatcgcacc tgcggattgc
     1681 cccatgctga ttgccgtttc ggtatttttc ggccgccaga ctttaaggaa taaagcgagt
     1741 gagacgatac tcaccagcgc cgaagtaata tccggcagtt ccggaccaat atagttagag
     1801 gtaaagaact gagtgacagc gaagcttccc ccagcaacca gcgccgctgg ccacgtctct
     1861 ttcacccctt tccagccgtc catcattgct accagccaga acggcacaag aaccgacagg
     1921 aacggtaact gacgtcccgc cattgcgcca atgtggaacg gatcgattcc cgttacctga
     1981 ccggcgacca gaatcggcac gcccaacgca ccaaacgcca ccgtcgcacc aaagccagca
     2041 gctccttcca gcaacgcacc aaaggagaaa ccaatcagta acacctgcaa acgctgatcg
     2101 tcggtgatgg agataaccga gctgcggata atatcgaact gcccgctggc aacggttaat
     2161 ttatacagga acaccgccgc gacaataatc cacgcaattg gccataatcc ataaataaag
     2221 ccatagcccg cagcagcaaa tgccatatca atcggcattt taaaggcgaa tatcgcaatc
     2281 aggatagata atataagggt tattgctcca gcaacatgtc ctttcagacg taataccgcg
     2341 agtgcaacga agaagaatat tatcgggatc agggcgacca gagcggatag ccccagtcct
     2401 cccatcggca tatacatttg ggtccaggta accatattgt tcagtctctt attatcttta
     2461 tatgcttgat atacttaagg ttgtaataag caaaagagga ctgaactgta aaatataggc
     2521 gttatacttt acagcaacag tacgccgcta acgcaattgc tacctctggc ataacaagta
     2581 tatcgggtaa gggtttctgt tccgcacacg caggcgcaga gtatcgttaa gatgtccata
     2641 ttgttgtttt aggcccgcta gtaatgcgct acgggtattt aatattgtta aaccctgata
     2701 atcgctccgg ttatttccgg gataaatgta ctaccgcagt tactatcata gccccgacaa
     2761 taaaacttgc cggggctttt ttgacgctat taatgacttt ctttttcgcg taaacgccag
     2821 gcgtgtaata acggttcggt ataaccgttt ggctgtttca cgccgaggaa gattaaatcg
     2881 ctggcagctt taaaagcaca cgagttagcg aaattccccg ccatcggacg ataagccgga
     2941 tcgccagcat tttgctgatc aaccactttc gccatattct ccagcgacgc ctgcacctgt
     3001 tctttggtca gaataccgtg acgtaaccag ttggcgatat gctggctgga gatacgcagc
     3061 gttgcgcggt cttccatcag cgccacgttg tgaatatccg gcacttttga acaaccaatc
     3121 ccctgctcca cccaacgcac cacgtacccc agaatcccct gcacgttgtt atccagctct
     3181 tgttggatct cttgcgccga ccagttagcg ttttcagcca ccggaatagt cagcagatcg
     3241 tccagcagcg gttcaaattc agcactgaac tcggtctggg caatgttggc ttgcacgctc
     3301 tgtacgttgg tatggtggta gtgcagcgca tggagcgtag cagcggttgg tgacggaacc
     3361 caggctgtgt ttgccccggc acgcagttgg tcgcccttct ggctgtacat gtctgccatc
     3421 aggtccggca ttgcccacat gcctttaccg atttgcgctt taccgcgcag cccacagaac
     3481 agaccggaaa gcacgttatt acgttcgtag gctttgatcc acggcgtcga tttcatctgg
     3541 tttttacgca gcatcgggcc agcttccatc accgaatgca tttcatcgcc ggtacggtcg
     3601 aggaaaccgg tattgatgaa cgccacgcgg ttgcgcgcct gagcgataca gctacgcaag
     3661 ttcagcgagg tccggcgttc ttcatccata atgcccattt tcagggtatt cggtgccata
     3721 ccgagcattg tctcaatgcg ggtaaacagt ttgttggcga acgccacttc ctgcggaccg
     3781 tgcattttcg gtttcacaat atagacgctg ccagtgcgcg agtttttctg cacttttaaa
     3841 tcatagaggg caatcgcgcc agtcatgacg ccatcaagaa taccttccgg gatttcattg
     3901 ccttcgctgt cccaaatcac aggaatggtc atcaaatgac ccacgttgcg gataaacagc
     3961 aacgagcgtc cgtgcagaga aatttcagag ccatcggcgg cggtgtactg acgatcgtca
     4021 ttcagtttac gtacgatttg ccgaccgttt ttctccattt tctcttgcag agtcccctgc
     4081 atcaggccca gcagattgcg gtacagcagg attttatctt ccgcatcaac cgccgcgacc
     4141 gaatcttcgc agtcgagaat ggtactgata gcagcttcga cgataacatc gttgatgtgc
     4201 gccggatcgt ctttgccaat ccgcccgttg gcatcaattt gcagttcaat atgcaggccg
     4261 ttatttttca gcaaaatgca ggtcggcgca gcggcatcgc cacggtaacc gacaaactgt
     4321 gctggagtac gtaacgtggt ttctttacca tttttcaact ggatgcgtaa ttgtttatcg
     4381 accaccttaa acgccaccac atcctgatag ctgccgtttt ccagcggtag tgattcatcg
     4441 aggaaacgtc gaacccaggc gataacctgc tcaccgcgct gcggatcgta gccactgacc
     4501 atcgcccctt cctgcgggat gatgtcgctg ccgtataacg catcgtacag tgagccccag
     4561 cgagcgttcg ccgcgttcag cgcgtagcgg gcgttcattg ccggaaccac cagctgtggc
     4621 cccgcctgac tggtgatttc gctgtcaatg cccgtggttt ccaccgtcac gcgctccggt
     4681 tgcggcacca ggtagcccag ttcacgaagg aaagatttat aggccgcttt atcttttacc
     4741 ggccccggat tgctgcgatg ccactcatca agcgccgcct gaatgcgatc gcgttctgcc
     4801 agcaactgac gattttctgg tgccagatcg tgaacgatct catcaacatt gtgccagaat
     4861 gcagcagcgt ccagttccac gcccggcaaa acttctccat ccacaaaacg tttaaaattg
     4921 gcgtcaatgc gtaaacggcc ctgggttatg gtttgactca ttgtttctct cctcgttttc
     4981 gcttatttcg ccaacaccgc tgctgccact ttcgcgacct gcgcatcctg tgctccggtt
     5041 aaaccagaaa cgcccacggc accaataatt tgcccatcaa caacaaccgg tacgccgcct
     5101 tccagcgacg tcagtaacgg cgcagtcacg aacgcggtac gtccgttgtt caccatctct
     5161 tcatagccct tagtttcacg acgccccagc gcggcggtac gcgctttctc ctgggagatg
     5221 taagccgcaa tcggcgcgca atcgtccatg cgacttaacg ccagcagatg accgccgtca
     5281 tcggcaacag caatggaaac agaccagtta tttttctgcg cctcttcctg acctgcggca
     5341 ataattgcac tcgccatttg ctggctaaga atgactttag ttttcatttt tttattcctt
     5401 ttcaagggct tgttctacaa tttcaatcca gtgacgcaca gaggtacgac cggcgctcgc
     5461 cagatgcgtc tggcaaccaa tgttggcggt gacgatcatt tccggtttgc cgctttccag
     5521 cgcattcatt ttgttatccc gcagctggcg tgccagatcg ggatgcgtta acgcatatgt
     5581 tcccgctgaa ccgcagcaca gatggctgtc gggaacgtcc gttaaggtaa atccaagacg
     5641 aagtaacact ttttccactt cgccgttcag cttttgcgca tgttgtaggg tacacgggca
     5701 gtggaaggcc agttttttat cgccgcgaat tgccagtttt tccagcggtt cctcgcgcag
     5761 aagttcgact aaatcgaccg ccagttcact gacctgacgc gctttatcgg catataacgc
     5821 atcgtttttc agcatctgcc catactcttt gacgaacgcg ccgcaaccgc tggcggtttg
     5881 caaaattgcc tcggcacctg cttcaatcgc gggccaccag gcatcaatat tattgcgcgc
     5941 ccgtgccaac cctttctcct gcgcattaag atgatagtcc accgcgccac aacagcctgc
     6001 ttcgttagct gacatgacgc tgatccccag acgatccagc actcgcgcag ttgccgcgtt
     6061 ggtgttgggc gaaagcgtag gctgggcgca gccttccagc attaaaaccc gacgcttatg
     6121 gcgcagcggc ggacgcggtt tagctttcac cgtttcagca ggcagttttg ctctgacctg
     6181 ttccggtaaa aacggtcgca gcaccagccc tacctgcgtc agcgcacgga agaccgccgg
     6241 acgcggcact acctggcgca atccttcgcg cagcattcgc tccggcagtg ggcgtttcac
     6301 tttctgctcg acaatatcac gcccgatatc cagcaaattg tgatagcgca caccagaagg
     6361 acaggtggtt tcacaattgc ggcaagtgag gcagcgatcg agatgctcct gtgttttaag
     6421 cgtgacttcg ttgccttcca gcacctgttt aatcagatag atgcgcccgc gcggcccgtc
     6481 cagttcatcg cccagaagct gataggttgg gcaggttgcg gtacaaaatc cgcagtgaac
     6541 acaggcgcac aggatgctgt cggcttccag cgcgcgttct gccgcatctc ttcggttaat
     6601 cgggtttgca tagcctgctc ctcaaagttc cgcgtacatg cgaccggggt taaacacgcc
     6661 gcaagggtcg agctgctgtt taagctgctg gtgatagcgg aataaaggag ccgatagcgg
     6721 ggcaaagcca ccatctccgg cactaaagcg ggtcgcatga ccgccagcgt tgcgggcgat
     6781 gcgatggatt tgattgtcat cggctgtcga tttcagccag cgtaacgccc cgccccagtc
     6841 gatcagttgc tcgccgggta aatccatcat cggcgcatcg ctgggtaatg aaatgcgcca
     6901 taaggtacct ggtaacgaga agaacggcag ttgttgttca cgcaattgct gccagaactg
     6961 acccacaacc tcttcgccac ccagcagttc acgcgctgct tttaccgatc cttcgccgcc
     7021 ctcaaggcgg atccacaacg cattgtcgaa gtaacataag ccactaatgg gtaatggctg
     7081 gagttgccac tcggcgattt cactcatggc ttcttgcagg ctgatttccc gacgcaggct
     7141 cagagaggca cgcggtcgcg gtaacacttt cattgagatt tcagtgagca cgccaagaca
     7201 accgtagctt ccggccatta accgtgagag atcgtatccg gcaacgtttt tcatcacttc
     7261 gccaccaaaa cgcagatgtt ttccagtgcc ggtaatgatg cgcgtgccga ggacaaaatc
     7321 gcggaccgaa ccgctccacg ggcgacgcgg ccccgccagc ccgcaggcga ccatcccgcc
     7381 ccaggtggct tcttcaccat aatgcggcgg ctcgcaggga agcatttgcc cggcgctctc
     7441 cagcgccgct tcaatcgcca ccagcggcgt tccggcacga gcggttatca ccagctcggt
     7501 cgggtcgtaa ttaacaatgc cgcgatgaca acgcacatcc agcgtttgcc cggtgactgg
     7561 gcgacctaaa aaggctttgc tattgctgcc ctgaatcacc agcggcgttt tatcgctaat
     7621 tgcctgattc acctgctcca gcagcgcctg actgtaatca cactcgcgta gcatcaaaag
     7681 cgctccagtt caggaaaagg taaatgaccg tgatgcacat gcatggcacc aaattcagca
     7741 cagcggtgta gcgtgggaat gtttttccca gggttcagca aaccatcggg gtcaaacgcc
     7801 gccttgaccg catggaaggt cgtgatttca tcgctgttga actgggcgca catttgattg
     7861 attttttctc gcccgatgcc atgttcccca ctgatgctgc cgccaacttc aacgcagagt
     7921 tcgaggatct tcccgcccag ctcttccgcg cgggcaaatt caccaggttc gttggcatcg
     7981 aaaaggatta acgggtgcat gttgccatct ccggcatgaa agacgttggc aacacgtaaa
     8041 tcatattgct gcgataaacg ggcaatgcct tccagtacgc caggcagggc gcgacgcggg
     8101 atggtgccat ccatgcagta gtaatccggg gagatacgtc ctaccgccgg gaacgcattt
     8161 ttgcgaccgg cccagaaacg tacgcgctct gcttcgtcct gtgccaaacg gacgtcagtc
     8221 gcgcccgctt tcaacaagat gtagttaacc cgctcgcagt cttcctgtac gtcagactcc
     8281 acgccgtcca gttcgcataa caaaatcgct tcggcgtcga cgggataacc ggcatgaata
     8341 aaatcttccg ccgcgcggat cgacaggtta tccatcatct ccagcccgcc ggggataatg
     8401 ccattggcga tgatgtcacc aaccgcaagt ccggcttttt ctaccgagtc aaagctggct
     8461 aacagaaccc gcgccacggg cggcttcggc agcagtttta ccgtcacttc ggtggtcacg
     8521 ccgagcatac cttccgatcc ggtgaacagc gccagcaggt caaaaccagg tgaatccagc
     8581 gcgtccgatc ccagcgtcag cgcctcgccg tccagcgttt gcacttcaat tttcagcagg
     8641 ttatgtacgg tcagaccata tttcaggcag tggacgccac cggcattttc cgccacattg
     8701 ccgccaattg aacaggcgat ttgtgaggaa ggatccggcg cgtaatagag attatgcggc
     8761 gcaacggcct aggagatcgc caggttacat acgcccggct gcacgcgcgc gcggcgacca
     8821 acggggttaa tgtcgaggct ctctttaaag cgcgccatca ccaacaacac acctttttcc
     8881 agcggcagcg cgccaccaga aagcccggtg cctgcaccac gggtcaccac cggtacacgc
     8941 aggcgatggc agacagccag aatcgctgtc acctgttcca tttgcttagg cagaacaacc
     9001 agtaatggac gcgtgcgata cgcgctcaac ccgtcacact cgtaaggaat gatctcctca
     9061 tcggtatgca ggatctcaag tccagggaca tgctcacgca gtgccatcag taccgatgtg
     9121 cggtcgacat cgggtaaagc gccatcaaga cgctcttcgt acaagatgct catgagtagg
     9181 cttcgctttg ttgtgttgtg tggcagctga tttttgcgcg ctgcttctgt gaacagttat
     9241 taagcgggct tttcgttttc gtctatctct ttagctaccg gtcagaccat tttttttcca
     9301 gctctgtgac cttgtcttgg ttaacttaat gttaaattga tgtaacataa tcacttgcgt
     9361 gatgtgcgtg ttttgcgagt taagaacaga aaaattggtc ctacctgtgc acgaggtccg
     9421 ggaatgaaag atgaacgtcg ccctatttgc gaagtggttg cagagagtat cgaacggtta
     9481 attatcgacg gcgtactgaa ggtcggtcag ccgcttccct cggaacgtcg actgtgtgaa
     9541 aagctcggct tctcacgctc cgcactgcgt gaagggctga ccgtgctgcg cgggcgcggg
     9601 attattgaaa cggcgcaggg tcgcgattct catgtcgcac ggcttaatcg ggagcaggac
     9661 accagcccgc tgatccatct gttcagtacg cagccgcgaa cgctgtacga tctgctcgac
     9721 gttcgcgcat tactggaggg cgaatcggca aggctggcgg caacgctggg aacgcaggct
     9781 gattttgttg tgataacccg ctgttatgaa aaaatgctcg ccgccagtga gaaccacaaa
     9841 gagatttcgc tgatcgaaca tgcgcagttg gatcacgctt tccatctcgc catttgtcag
     9901 gcttctcaca atcaggtgct ggtgtttacg ctgcaatcat tgaccgatct gatgtttaat
     9961 tcagtgtttg ccagcgtaaa taatctctac catcgaccac agcaaaaaaa gcagatcgat
    10021 cgccagcatg cgcggatcta caacgcggtg ttgcagcggc tgccgcacgt cgctcagcgc
    10081 gcagcgcgcg atcatgtacg gaccgtgaaa aagaatctcc acgatatcga gctggaaggc
    10141 caccatttga ttcgctcggc ggtgccgctg gagatgaaca aggtgggaat gtgagcagag
    10201 ttttacccta tccccaaggc gaggggaccg cccctgcact taacaggatc tggtgttaaa
    10261 tctgttcatc tataccctct cccgaatgac cgcccctgcg ctaaacagga tctggtatta
    10321 aatttgcttt tcatacaatc ggtaacgctt gtacggctcc gccccaatgc gttccagcat
    10381 gttattcatg cctgtattgg tttcgaggat ccatgacatc tccagcgcat cgatcttccg
    10441 gcgggcaaac ggatcgcgta aggcttcaat caataacagc gcaatcaccg ggccgatgcg
    10501 gctgaactga tactcgtcgc gcacgcccat cagcggtact cgcgcagttc gcacaccgct
    10561 gactttcaaa cgccacagca attttgccca gccgaaggga aagagcgatc cgttcagatc
    10621 ggcaatcgcc tcgttgatgt taggcaagcc acaataaacg cgcagggtgc agaatcaatc
    10681 tcagcgatgt agatcatatc gtccggcacc agatatttaa gttggtcgcc catggtcgcg
    10741 aattcatgtt cggtaaacgg cacaaatccc cagttgtgct gccagccaga gttgaaaatc
    10801 tcacgcagga tctgcatctc ttcggcaaac cgctgacgat tgatgcagcg aatggtcacc
    10861 tttttgcgca cctgatccat cagttttttt agcgccggag agaaagtgag atcggttcgc
    10921 tgcatccacc acgccagtaa atcaatgcct ttgtgataac ccagttgttc aatatgcgcg
    10981 gcataccacg gtttgccgtg tggcatcatc gcacagggtg gtgtgtcaaa accttcaatc
    11041 agtaatccgc tttcctgatt gatattcagg ctgaaaggcc cgctgatctt acttgcacct
    11101 tgtgacttca accacgcttc cgctgcgcca aacaacgcgg caaaatcctg cggatcatca
    11161 atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt aacgctcacg gtgcaaggta
    11221 tctatttgcg cggtaatacg cccaactatc tgccccgctt tttttgccac ccacgcctgc
    11281 cagatgatat ggtccgtccc cggatttttc gcagacaaat gctcgttgcg ttcaatgaat
    11341 aaagggggta tccagtttgg atcgtcggga taaagtgatg acggaaaagc gataaatgcc
    11401 ttaaggtcat ttttattaat tactttttca attttaatca tcagcagatc gcattccata
    11461 tctctgtgga taacaaataa ttaatggtga aaaacaggtt gcaacagcaa cgagcggcat
    11521 cttaataatc aactcaatga atacaaacga cgaataaata gtattaatta atgattatga
    11581 taaggtcaga ggctacacag aaacgcctca atgccttttg tcaatttaag gcgaccggac
    11641 aaggcttctt cttaatatat tcataaatta aatatcattc ataataaaaa aaacttaaac
    11701 tcattattta aatcaatgtt cattttcaca ccgatgaaaa tactatctca tttatctttt
    11761 atgcctatat aatccaccgc attattgtca ttttattttt gatatccaat tttttcgtgg
    11821 tgtatatgtc taataaaatc tttacgcatt ccctacctat gcgctatgcc gattttccaa
    11881 cgctggttga tgctttggac tacgccgctc tgagtagagc cggaatgaat ttttatgaca
    11941 gacgttgcca acttgaagat caactggaat atcagacatt aaaaacgcgt gccgaagctg
    12001 gtgcgaagcg gttgttatcg ctgaacctga aaaaaggcga acgcgtggcg ctgattgccg
    12061 aaacgagtag cgggtttgta gaggcttttt ttgcctgtca gtatgctggc ttagtcgccg
    12121 tcccgttggc gattccaatg ggcgtcggtc agcgggattc ctggagcgcc aaactccagg
    12181 gtttactggc aagttgccag cccgcagcca ttatcactgg tgatgagtgg ttgccactgg
    12241 tcaatgccgc gacgcataac aaccccgaat tacatgtttt aagccacgct tggtttaagg
    12301 cattaccgga agccgatgtt gcgctccagc gtccagttcc gaacgatatc gcctacctcc
    12361 agtacacctc cggcagcacc cgttttcccc gtggcgtcat tatcacccat cgcgaagtga
    12421 tggctaatct acgtgctata agccacgatg gcattaaatt acgccctagc gaccgctgcg
    12481 tctcctggca gcctttctac catgatatgg gactggtcgg ctttctcctg ccccccgtcg
    12541 ccacgcaact ttcagtagat tatttgcgca ctcaggattt tgccatgcgt cctttgcaat
    12601 ggcttaaatt gatcagtaaa aatcgcggca ccgtttccgt tgcgccgccg tttggctatg
    12661 aattgtgcca acgccgcgtg aatgaaaaag atctcgctga actggatctt tcctgctggc
    12721 gcgtcgctgg tattggtgct gagccgatct ccgcagaaca actccatcaa ttcgctgaat
    12781 gtttccgtca ggttaacttt gacaataaaa ctttcatgcc gtgctacgga ctggcagaaa
    12841 atgcgctggc tgtcagcttc tctgatgaag cctccggggt tgtggttaac gaagtggatc
    12901 gcgacatcct cgaatatcag ggtaaagccg tcgcgccggg tgcagagaca cgcgccgtat
    12961 cgactttcgt caactgcggc aaagcgttgc cggaacatgg tattgaaatc cgcaatgaag
    13021 caggtatgcc ggtcgcggaa cgtgtggtag gccatatttg catctccggt cccagtctga
    13081 tgagcggtta ctttggcgac caggtttcgc aagacgagat tgccgcgacg ggctggttag
    13141 acaccggcga cctcggttat ctgctggacg gttatctgta tgtcaccgga cgcattaaag
    13201 atctgattat tattcgtggc cgtaatatct ggccgcagga tattgaatat atagcggaac
    13261 aggaaccgga aattcattct ggcgatgcga ttgcttttgt taccgcccag gaaaaaatca
    13321 ttttgcagat ccagtgtcgg atcagcgacg aagaacgtcg cgggcagctt atccacgcgc
    13381 tggcggcacg gatccaaagc gaatttggcg tgaccgcggc tatcgatctg ttgccgcccc
    13441 acagtattcc ccgaacgtcc tccggcaagc ctgcccgtgc ggaagcgaaa aaacgttatc
    13501 agaaggctta tgctgccagt cttaatgtgc aggaatccct ggcataaatc aaactgtcgc
    13561 ggtgacgggc gctaccgggt ttatcggtaa atatattatc gataacctgc tcgcccgcgg
    13621 ctttcacgtt cgcgcattga cgcgtactgc ccgcgctcac gtcaatgata atctcacctg
    13681 ggtgcgcggt tcgctggaag atactcattc acttagcgag ctggttgccg gagccagcgt
    13741 agtggtccat tgcgccgggc aagtgcgcgg gcacaaagaa gagattttca cccgctgtaa
    13801 cgttgacggc agcctgcgcc tgatgcaagc agcaaaagag agcggctttt gccaacgttt
    13861 tctgtttatc tcttcgctgg cggcgcgcca tcccgagctc tcctggtatg caaaatccaa
    13921 atacgtcgcc gagcaacgac tggcagcaat ggcggacgaa attacgctgg gcgtttttcg
    13981 cccgacagcc gtgtatggtc ccggcgataa agagttaaaa ccgctgtttg actggatgct
    14041 acgcggcctg ctgccacgac ttggtgcgcc agatacacaa ctctcttttc ttcacgtcac
    14101 cgattttgcg caagcagtgg gtcagtggtt aagcgccgaa actgtacaga cgcaaaccta
    14161 tgaattatgc gatggcgtcg ccggtggcta tgactggcaa cgcgtacgac agcttgccgc
    14221 caaagcccgt tgtggttccg tgcgaatggt tgacattcct ctgccggtac tcacaggcct
    14281 tgcggatatc agtaccgcgt tgagtcgcct ggcaggtaaa gaacctatgc tgacccgctc
    14341 gaaaattcgt gaattaaccc acgccgactg gtcggcaagt aataaccgta tttctgaaga
    14401 tatcaattgg tttcccggga ttagcctgga acacgcatta cgcaacgggc tattttgatt
    14461 gtcttgtttt aaaggtgtgt taacgtttct atggtaaatc gtgaaatagt aatggattac
    14521 atcctttcct gtttacagga tttggtcgaa aacggtgtgg aaattaagcc cgacagcgat
    14581 ctcgtcaacg atcttggcct ggagtcgatc aaagtgatgg atctcttaat gatgctggaa
    14641 gacagattcg atatttccat tcctattaat attctgctgg atgtcaaaac ccctgcgcaa
    14701 ttaatggaaa ctctacttcc atggctggag aataaataat ggggctatac gataaatatg
    14761 cgcgccttgc tggcgagcgt ctgcaattta gtgacaacgg gttaacaccg tttggcacct
    14821 gtattgatga agtgtattcc gccaccgaag gccggatcgg caataaaaaa gtgattctgg
    14881 ccgggaccaa taattatctc ggtttaacct ttaatcatga tgccatcagc gaaggtcagg
    14941 ccgcgttagc ggcccaaggc accggcacca ccggttctcg tatggcaaac ggcagttatg
    15001 cgccgcacct ggcgttagag aaagagattg ccgagttttt caatcgccct accgctatcg
    15061 tttttcaacc ggttataccg ctaatttagg cgtcatcagc gcacttgccg accataacgc
    15121 cgtggtgtta ctggatgccg acagccacgc cagtatttat gacgtctgtt ctctcggtgg
    15181 cgcggaaatt attcgtttcc gccataacga tgctaaagat ctggaacggc gcatggttcg
    15241 cctcggtgaa cgggcgaaag aggcgattat catcgttgag ggcatctaca gcatgctcgg
    15301 tgacgttgcg ccgctggcgg aaattgttga tatcaaacgt cgtctcggtg gttatttgat
    15361 tgtcgatgaa gcgcattcgt ttggcgtgtt aggcgcaacg gggcgcggac tggctgaagc
    15421 tgtcggcgtt gaagatgatg tggatattat cgttggtact ttcagtaaaa gtctggcctc
    15481 gattggcggt tttgctgtcg tttcggaagc gatggaagtg ctgcgctacg gcagccgccc
    15541 gtatattttt accgcctctc cttctccgtc ctgcattgcg accgtgcgct cgtcgttaag
    15601 aactattgcc acacaaccag agctgcggca aaagctgatg gataacgcca accatctgta
    15661 tgacggtctg caaaaactgg gttatgagtt aagttcacat attagcccgg tagtgccagt
    15721 aataatcggt tcgaaagagg aaggtttacg catctggcgc gagcttatct cgccgggcgt
    15781 gtatgtgaat cttatcctgc cgcctgccgc gcctgcaggc atcacgctgc tgcgctgtag
    15841 cgtaaatgct gcccacagtc gtgagcaaat cgatgccatc attcaggcat ttgctactct
    15901 gaagcagtaa tgtgagcccg ccgtggtgtt tgcgccacgg cgggtttaat gttgtctgtc
    15961 ataccaatac accaggctca acgccaccaa cacaaacgct accggtaaac cgagcgcggt
    16021 aacaggtgcg cttaacgcaa atagcaatcc cagattaacc atgatttgat agctgatcca
    16081 ggtgagtaag cccacgatga cgcctacagc gagacggcta cccatcccag gcgaacgcgg
    16141 ggcactaaag gtaaacggca cagccagcaa aatcatcgcc agggtcagga tgggttgccc
    16201 cagtttttcc cacagtgcaa ggtggtattc gctgctgggt tgcccggttt cctgcaaata
    16261 atggatgtaa tgattaagct gacggacaga aaaagtattc cccggcattg ataactcttc
    16321 aagattcata ctggtgaagg ccgattgcca ggcaagatta tctaatgttt ccagcgtttc
    16381 tttgccattc agccattttt tatgattcac accatgcagg acccacgttt tgtcgtcttc
    16441 aatggtggca ctgcgtgcat aaatgtagga ttcaagcgag agatcgtcgc gataatgaaa
    16501 tatctccacg cccaccggct ggccctgctc gttcagcgat ttcaccgtca caaattcatt
    16561 accacgcctg gcccacagca tattgccggt aatgtcatcg tcctccccca acgcggtggc
    16621 ggtcgatttg atttgcaacg cctgttgctg caatggcgac gccacccact catctatcgc
    16681 gcctaacgaa acagtccaca atatcccggc aaccagcgcc accagtgcaa tacggaagat
    16741 agaaaacccc gtgctgcgaa tggcggtaag ctcactgttt ttcgataact gccccagtcc
    16801 aacaatacca cccaataacg cgataaacgg cgaaagttcg accagtgtgc gcggtaaggt
    16861 cattagcacc accagcaccg cctgagtcca gcgataaccg cccgggctga caccatccag
    16921 ttcgttaatc aggttgaagg tggtaaaaag cggcagcaat agccctgcag cggcggcaaa
    16981 accgagaaag agatgacgga ttaaatagcg actgaaaaca ttcatcggga aaatttctgc
    17041 agcaaagaga agtcacgtgc gaccagtatc agcaggccca ttagcatgag tccgggcact
    17101 aaccacaaac caggaatgag gggaatcgcg ccgttagcca ccagtgtacg gcagacgtcg
    17161 ccgccataaa aaatggcaat aaacagcaac gtcagtggca ataacgttga aaatcgccct
    17221 tgccgcggtt ttacccgact taatgaaatg gccagcagcg ccatcaacaa tgcggtcagg
    17281 ccacggcttt gtcgccattg cagttcggca tgatcggcag gaaaggcgga gcgcgccagc
    17341 tccgtgaccg atttcgcttt gcgtttgacg ttcgggcttt gatccagcgg attcagatgt
    17401 aattgcagat tacggtagat ctgttcgttg tcgtcacgcc cctgatgatc gagaagatag
    17461 gcagtgccgt tgtgcaacat aacggtcggt ttctcgggtg atgggtcaac cacatcaacc
    17521 gaaccggcgc ggaaaatacg ggttcgattg gcagtagaag tataaatcag cgcgtcagtc
    17581 aggcgattat tatcctgatc aaccgtctgc gaaaggatca ttcgtccgtt atcgttagtg
    17641 ttaaatttct ttgcccgcaa ctggcgaaca tccagctccg actgcgactg ctgctccagt
    17701 tgataaatct gcgcataagc ccacggacga ccatacatcg acagaagggt gacaaaaata
    17761 cttagcggta tcgccagata aagaacggct ttgtacaagc gtcccggact gccccccgcc
    17821 gcagagatag cggtaatttc cgagtcggta tacatctgcc ctagcgtcac gccaactgac
    17881 acatacagac caacaggtaa caacatctcc agtgcaatca gtactttgta gaaaacgata
    17941 tccagcacaa catcaagagc taatgtccca tttgccgctt cggtcagata acgctgtgcg
    18001 gagtagctgg cgaaaataaa gatcagaaaa ccgacgataa tcagcaccag acggcgcgta
    18061 ccacgcatga tgtagtgttc aaccagcttc atctcactaa cgccctcaat cctttgagtt
    18121 ttttattcat tgatcagttt ctgcgccaac gcaatgacat gatctacctc ttccggcgtc
    18181 aggctgccgt cctttgccca ctgaatttgc ccggctttat tcagcaccag aatggttgaa
    18241 ctctgctccg gcaagcgcca ggcaacgcgt ccctgtccat tgccgtcaat aacgaattgc
    18301 gcccacggat agcggcgttt atttttttcg attttgccaa caacaaaata gccagtacca
    18361 aagatggcat catcggtgtt cacaatggtg gtgggctgaa aacgatcctg cggaaagttt
    18421 gccgcttcaa ccgctttaat taacagtgag ttctttttct tcgccgactt acgcccggcg
    18481 atatactgaa taatgcggac ttttcccgct aattgtgtgc tgttccactt ttggtagtga
    18541 atttcgtcgt cgctattcag aagcagctcg ccgccttcct ggatataaac aggggtaacc
    18601 gttttgccag tgacgaaatt gtgtgcctgc acacccgggg ctaagagcat gataatcaac
    18661 gcaataatca aacgagatga catcattgct ccggctatag cggaaaacgc gaaatcagca
    18721 gctctaagcg ctgacaaata tcgctatcca gcgccaacgg tgcataatac gactggcggt
    18781 tttccatatc atccgccacc atgtcgtcgt gtttatctcc catcaggcca ttaatggcgg
    18841 cgaggaaacg ggaaatctgt aacgcctctt tattttcagg atcaagcgat cttaaggtgt
    18901 cgtaataaga aacctgctgg ctgatgattt ttcgcacatc atgaaattcc ctgccggtta
    18961 gtcgcggttt ggcaataatc tctttcaggg tatgcatttg cttttgccga taggcaataa
    19021 aactgcttac gctttctaac tggctatggc gtaatgaata gttcaccaac gaccagaccg
    19081 gagagcttaa ataaatccgt aacaggttgc cgtaatacga cacaatattt ttcttaccgt
    19141 tacgaaagcc gtcctgaaaa cgccccagaa aaacggtcgt tttgccgaat aaaaatccgg
    19201 cctgatgctt ctttaaatac agccgttgcg caaaacgcag gtgcttgtag cgcgctcgca
    19261 tgtatttaaa ttgtttgcgc tcatcagcag tcagctcatc gcctttggct tgtttgcgaa
    19321 ctaaacgcag taattcctga cgatcaacgc cattgagcca gaattgcagg cataacgcgt
    19381 aattatcatg aatgatttcg gtggtacaac ttagtgcaat agtcggcggc aaactaacct
    19441 gtgcatcaac gacatcatct tcttcaatcg caacaaatag acgctcgctc gcctcaagcg
    19501 ggaaatattt tctcatcatc aggatttctc tccggctgcg ttataaagca aggaatggcg
    19561 ctgaaaaaat gcctttaagg ttggaattaa cgagagcgcc acgtccagac attgcccacc
    19621 gaagtaagca aacgctgctc ccagcacacc aaacagatga cagaacatga acagcaatac
    19681 gatgtagccg attgaggcga tggtttgcgc cacgagaaaa gcgcgctgtt ttcccgccat
    19741 caatagcaat gactcctgcg ggaagcccag catcgaaatc acaattgcgc ccagcatcac
    19801 ctgaatcagg tcatacgctt cgagatactt aacgccaaac accagcgaaa tgagcggctt
    19861 gccgacgatc aacaccgcga gcgccaccag aataccgatt ccgcccgcca gtaaaccgga
    19921 cttcacacct aacaaccacg gtctggtggt gcgcggatct aaacgcatca cctctgggta
    19981 aaaactttta cccagcaaac ccgctggcgt tccggcggcg tcgaagaatg tcatggcgat
    20041 tttaaataac ccggcggcag cgggtcctaa cacgatcccc accaacactg tgctgcacga
    20101 gttacgcgcc gaccagatgg agtgggcaat gttggttgac cagacaaaac tccacgcgcc
    20161 tttaatgtgt cgggcagact caaacagatt caatttgaag gcgttatgga tatttctacg
    20221 gcgtaattcg cgcgcggcaa accaccagta catggtgccg ccaaccagat tcgacacgta
    20281 ccaggcaata acaaaaccca caaaaccaaa gtcaaaatac caggctgcga cgctccccgc
    20341 tgcgcgcaga aaaggtttcg tcgcctgctg tacagcaatc aaatcaaagc gatctaccgc
    20401 acgcagaatg ccggtcggcg tggaggaagc cattgaagga atgagcgtgc aatacagcgc
    20461 cgccagccaa aagctttggt catccagacc taatgaatgg gaaaggaatg gcagtaaggc
    20521 aatgccgccg acaatcgcca ccgcgccgct gacgatatcc agcgagaagg aaaatgagac
    20581 gacattgcga aattgctgcg gattattgtt ggtcaaggct ggcgttccgt actgaaccac
    20641 cagctgccat gtctgaaact taataaaatc gctgatcgac ttggcgtacg attgcacaat
    20701 caccagtaca ccaaacatgg cgggcgtcat ccctttaccg gcacacgaga gcgccaacag
    20761 ccccagcaag gcgctctcga cattactgga gcctaaccag gcgctattgc gaataatggt
    20821 gcgaaacgcg ccatctgcaa accaatgttt gatgttaaaa cccgccaatt cagcctgacc
    20881 ttatcgttga tagtaaaaaa tatcccgcca gccttaagtt aaacttcggc ggtcagaaac
    20941 gatggcaacc agagaaaccg ccttctgtac ctgttccagc acttcgctgt agggcgctct
    21001 ggaatcaatc tcaagaattt ttgtgccgtt atagccaatc gtcgacatga cgccgatttt
    21061 gtcctgcagc tcggcatact catggtcagg cttgcgggag atggcagtat caatatcaat
    21121 ccccaaacga atgattaatt caggacggta ttgcgccatt ttttggtata aactccgttc
    21181 gcgctgcgcc agaaacatgc tgattttccc ggtcgcacgt tcgacgccga tccccggtcc
    21241 gtcataataa aagcccgaaa tttcagcctg cgggaagcga tcgctgacca ccagaacgcc
    21301 actttgcgcc agtcgctgaa ccttgcgtag attcgccatt cttcgcagcg agaagcagta
    21361 cataatcacc gccgcccata gcgccggaga tttggttttc atgctttggg ttttcgagga
    21421 tttggccgcc aatcgccgtt ccagccagac gccaaccaac ggcaatcgtt tgattttgtc
    21481 gccgtcttcg ccggagagca gccccagata gcggcgctcg gtttgccagt gttgttgcag
    21541 cgatttcacc aggtcggtgg tcagcgtgga tttaccggtg ccatcacacc cgaccaccgc
    21601 aatcagcccc ggaatgtagt tgggctgcgg cgcggttgta ctattaacag tttgtgtttg
    21661 tagtgcatcc attcgttaaa tcatccccta tccattttgg tgatcaggaa agcgcggcgt
    21721 gaattgcgcg tagtgacgct tgcataattt catcggctgg ttgccgacca tccagttcaa
    21781 ggatcttggc gccattgaat gtcagttgcg gcgtaacggc gattttttcc tgtaacgctg
    21841 ccagttggtg gtcaggttta cgcgcaaacg cggtttgttc atcaatgcca agacgaatca
    21901 acaatacggg taaataagac gccatccatt ggtacagctt caactcgcgc tgccttaaca
    21961 tttttatcca accgttaccg cccgtggttt ttgccaattg cgggccatca aagcgaaaac
    22021 ccggcacttc aacctgcggg tagcggtcgg tgatgagcag atagccttgc tggcttttac
    22081 acaacatttt gcgaaacttg tacgcccgcc agcaggaaag cagatagata accagcgcag
    22141 taatattgcc aggcggagtt aagggctttt cgtgtacatg tgccgcttta cttcgcagat
    22201 aacacccaca aggtgcgcca ataacaggga gctgtgaaat ccattcgcca attcgcccgg
    22261 acgattgccc gagataaatg tgttctgttg gcattcttgc tgccagttca tttaccaggc
    22321 ttgccgtgag ggtcgattta cctgaaccat cacatcctac aatggcaata acacgcactg
    22381 gcgtggaatt aattattgac atattacgtt gattcacgaa aaacccggca gtaaataatg
    22441 tattgaatat tacgtggtcc gtgctcagaa tatccgttca accttattgt gacaatgact
    22501 aattgtgaca ttgacctgaa ttacgctttc ataaaaacat attaaccaaa taaatatttt
    22561 taatggatat ttaaattaaa ggatatattc atgcagtcaa taacacctcc attaattgcc
    22621 gttattggta gcgatgcttc aggcaagtca acggtgtgtg aacatcttat taccgttgtc
    22681 gaaaaatatg gtgctgccga aagagttcat ttaggaaaac aggccggaaa tgtcggtcgt
    22741 gcagtgacaa aattaccttt gatgggaaaa tccttacata aaacaattga acgaaatcag
    22801 gtgaaaacag caaaaaaatt gcctggacca gttccggcgc tggtaattac agcgtttgtc
    22861 gcccgtcgct tactgcgctt tcgtcatatg cttgcctgtc gtcgtcgcgg gttaattgtt
    22921 ctaaccgacc gttatcctca ggaccaaatt cctggcgctt acgatggtac ggtgttccca
    22981 cctaacgttg aaggtggtcg ttttgtctca tggctggcaa gccaggaacg taaagcgttt
    23041 cactggatgg cgagccataa gcctgatctg gtcatcaaac tcaatgttga ccttgaagtt
    23101 gcctgtgcac gtaaacccga ccataaacgg gaatcgctgg cgaggaagat tgccataacg
    23161 ccacagttaa cctttggtgg tgcacaactg gttgatatcg atgccaatca gccactggaa
    23221 caggtgttgg ttgatgcaga aaaagcgatt acggatttta tgaccgcgcg tggttatcac
    23281 tagtcaaaaa tggaaatgcc cgatcgccag gaccgggcat tttcaggaag gttaaatcaa
    23341 ctgcaatgct atccagtaca gcccaccaga aagaaaaatt gccgccggta aagtaaacac
    23401 ccacgccatc aggatgctgg ttaccgtttt acgctgtaac ccaccgccgt ccaccaccat
    23461 cgtccctgct accgcagacg agaggacgtg tgttgtggag acaggcatcc caatataact
    23521 ggcaaggccg atagacactg ccgccgtcat ttgtgccgcc atgccttgcg catacgtcat
    23581 gccgcgctta ccaatcttct caccgatggt catcgctaca cgacgccagc caatcatggt
    23641 gccaatgccg agcgccagtg ctaccgccat gatgatccac accggagcgt actcaatggt
    23701 gcttaacata tcgctgcgca gttttttcag caggttctgg tcttctttac tgacgcctgg
    23761 cagtttcgcc agcttcgcgg aggtatcgga gatgcacagc ataatgcggc gcagctggct
    23821 gcgctgactc acgcttaacg gctcgtaact ttccatattg cctggcagca tcgttttaac
    23881 gcgcgcaatc gcatcaaagg tatttgccgg atgacagtga aactctgtta cttgcgtgcc
    23941 atcagtcgat gctgcaggca atggaggttc catcgcaatc aacttctgcg gcagttcagg
    24001 atgctgttgc aggtagtgtt cgaagttggt aacggcatcg cgggtacggg taatttcata
    24061 gccggacgca ttcatattga cgacgaagcc agcaggggca atccccacca gtaccagcat
    24121 taccaggccg atccctttct gcccgtcgtt cgcgccgtgc gaaaacgcca cgcccgcagc
    24181 ggaaacaatc agcgcaatac gcgtccagaa tggcggttta cgtttgccct ttttcttttt
    24241 gcgatcttcc ggaatgcggt gaatacggtc acgctttttc gtcccactcc agaaacggcg
    24301 cagcaggaaa atcagaccac ccgcaatgac caggccgacg ataggggaaa caatcagcga
    24361 ggagaaaatt ttggttactt cacgcaggtt taacgcatcc atcaccgatg agccggttaa
    24421 cagcgcgttg gttaaaccga tgccgataat cgcaccaatc aaggtgtgcg aactggaggc
    24481 cggtaaacca aagaaccacg ttcccaggtt ccagataatc gccgccagca gcatggaaaa
    24541 gaccatcgcc aaaccgtgag ttgaccccat attcagcaac aaatcgattg gtaacatatg
    24601 gacaatggca taggcaacgc taagtccgcc caataacacg ccaaaaaagt taaaaaatgc
    24661 cgccatcacc acagcaagtt gtggctgcat ggcacgagta taaataacgg ttgccaccgc
    24721 attcgccgtg tcatgaaaac cattgattgc ttcgtagaac aacacaaatg ccagagcaag
    24781 taataacaaa agccccgtat atatatcaag gccaacaaat aaatttagca tatattagat
    24841 tacgccattt tgaatttacg aacggacgca ttatcagtga ctttaacggc atgggcaaag
    24901 tgaaatatca ttttttaacg ataaaaagag tgttatttct tgtgtatcag acaataagca
    24961 ttcattcaaa acctttagaa ataatcaaaa cacaggtatt ccactggtta atatattcac
    25021 atatgaaatg aataaatatt ggaatatata aatattgaat attttgatta atccccgtac
    25081 taattattct tcataatcag tacggggttg ctacaacatg tattactttt tcaccacaat
    25141 taacggttca atatcactct cttttttgat gaccagtgat tcatcaccgc gcaaacacgt
    25201 cccaccgtag ttgccgccaa cggtgaaggt acatacctga atgtatttac cgtccacttt
    25261 cggcaaacac cacagttgct gatagatgtt tttctgctcg gcaaatttac cgctggtttt
    25321 gtccagcacc tcttcatgat ggctgaccag gtcgatattg ctaccacagc gaccggcgat
    25381 cggtttcact gcgtaacctg ttttcaccaa ttcatcatta acagtgaaat cggtatccag
    25441 caggtaacga tggtgcggga acagcgacca gaggatcggc agaatcgctt tgttgccggg
    25501 gatcaccgtc cacagcggct caaagaccag cacttccggg cgcagcaata cgtcgataag
    25561 acgcacttcg ttttgcggat gaccggtacg gattggcacc gcagcaaact cacggtcgct
    25621 aacttcacga atctgatcaa acgcggtttc ccacgcccag gttttccaca cgcagttaac
    25681 cagtcgccct tccccatcaa tcagttgccc ggcagcatcc cagcccagtt catccagccc
    25741 gcgcaagata cgcgtttcaa agcccgcctg gtgcagcgcc tgctccataa actgcgcgtg
    25801 atagttttcc tcgatatctt tgtcctgcat gatatggaca aacggacgtg cacgactgtg
    25861 tttccaggca ccagccagtt cgttaatcag cccttccgcc ggattgaagc cgttgccttt
    25921 atagccctgc tccgcccaac gttcgaggat caagcccgct tcggtatgac aggaggcgga
    25981 gtcggcgttg tattcgtaaa ccttcaggcc acgctcatcc atgcagaaat ccatacgacc
    26041 agtgatcata tggtgacggc gacgctgcca ggagagacgc aaacgtggcc agaggatttt
    26101 cggaatgtcg aacagcgcca gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag
    26161 atacatcaga tgcagctcgt tggtggcttt aattagctcc tgctcggcac tctcggtaat
    26221 ggtgtagtaa tgatacggat cctgattgat cacctgaccg ttggcctgca cataggcgtt
    26281 ttgtagcgga tctttttcat ccagccattt accgtcaaac tggcctttgt tttccagacg
    26341 cgctccactg attttcagca gctcgcctgc aatttccggc tgcggtaagc tgtattcagt
    26401 atcttccgtc tggatcatcc agcccagaat ggtggtgtca tcaaaagtgt ctttaagggt
    26461 atagcagccg ttttcgacca ccatctccag ctcgcgcgtc cactgttgcc cttgcggcaa
    26521 cggggaatga atcacgttct gttccgcaat acggactttg ttgccatgca attgggtaat
    26581 gatggcgaca tggccagtgt ctttaaattc accgccttta tcccagataa gaagcgcacc
    26641 cgcgaccggc gcacgcggcg agccgttagg aaatgcctgc aatggcagga tgttgtcatt
    26701 aaccacttct cgcaggaagc gcagcgagaa aatctcccac gccataccca cgtcagtaaa
    26761 gaccacaccg taattcagaa agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg
    26821 gcccatatat tcgtcgtcga tatagctacg gaatacggcg tcatcttcgt attcctgcgg
    26881 atcgagagaa ctgtaatctg aagagtagat tgctacccca cctggggcgt agcccaataa
    26941 tgtcccgaac ggggcatcct ggctggtcgt tcctttgctc atcactttac ctttaacaat
    27001 acaacctaag cagttggcgt aattgttgtg ctctgattac ctgctcagcg acattaaccg
    27061 gacagaggtc agactaatca tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat
    27121 tcacattctg tacagcaagt gacctgctac actgcttcaa cactaccact cagaaggcaa
    27181 ctcactatga cagacaatac ttatcagccc gcgaaagtct ggacgtggga taaatccgct
    27241 ggcggcgcgt tcgccaatat caatcgcccg ttttctggtc cgacgcatga aaaaacgctg
    27301 cccgttggca aacatccatt gcaactttat tcgctgggaa cgccgaacgg tcagaaagta
    27361 acgattatgc ttgaggagct actggcgctg ggcgttactg gtgcagagta cgacgcctgg
    27421 ctgattcgta ttggcgatgg cgatcaattc tccagcggct ttgtcgaagt gaacccaaac
    27481 tcgaagatcc cggcgctgcg cgatcatacg cataatccgc cgatccgcgt gtttgaatct
    27541 ggttcgatcc tgctttatct ggcggagaaa tttggctact tcctgccgca ggatctggca
    27601 aaacggactg aaacgttgaa ctggctgttc tggttacagg gtgccgcgcc gttcctcggc
    27661 ggtggttttg gtcactttta ccattacgcg ccggtgaaaa ttgagtacgc catcaaccgc
    27721 tttaccatgg aagccaagcg cctgctcgac gtgctggata agcaactggc gcagcataag
    27781 tttgttgcgg gcgatgagta caccattgcg gatatggcga tttggccgtg gtttggcaac
    27841 gtggtgctcg gcggcgtgta tgatgccgca gagtttctgg atgcgggaag ttataaacat
    27901 gtgcaacgct gggcgaaaga agtgggcgaa cgtccggcag tgaaacgtgg gcgtattgtt
    27961 aaccgcacca acgggccgct gaacgagcag ttgcatgagc gccatgacgc cagtgatttt
    28021 gagacaaata cagaagataa gcgtcagggg taagggttgg tgttcgtcgc agcaagccat
    28081 ccaggccgga taaggcgttc acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg
    28141 ctgccgcgtc ttatcaggcc tacaaaagct aatcatcacg cgctggtaat gtcgtaatcc
    28201 atttggcgca gtgcgtctaa tgtggctttg gcttcatctt cgtcgatgat gctcatggca
    28261 aatccgacgt gtaccagcac ccactggccc agcagatcgg cagggttacc ttcgcaaatc
    28321 agggcgatat tcacgtcgcg cttgatacca catacttcaa cctgcgcaag ctggtgaata
    28381 tcttcaccga cagccagcac ctggcctgga acgccaatac acattgttaa ctccggttat
    28441 ttcgattgcc ggacaaacgc gaaaaagaac ctttagcggg tgttccctct caccctaacc
    28501 ctctccccgg aggggcgagg ggaccgtccg tgcgcttttt aggcgttgtc ctcagtctta
    28561 ttcaacttca atacttttga cgatcagcga atcgccggta tcgacacgca accgctcgcc
    28621 gtgacagagc ggacactgcg catcgtgctg atgaatctcc accacctggc tgcaatccca
    28681 gcaccaggcc tgggcgggtt tatagacgat atgtaaatcg cacccttgcg ccaccgttcc
    28741 gtggcagaca atttcaaaac taaaacggac ggcgctctcc tcaacgcagg agagcgcgcc
    28801 aatttccagc cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct gccgttggat
    28861 aatttcaacg gcgctctggc aaagcgacaa ctcatgcatt ttcgcccctc ctgcggccaa
    28921 acagcagggc gcgacgccct gcgtgtggta catccggatt ggtgactggc aacgaaagga
    28981 tcattcgtgc gcagtcatcc gtcaggcgtt ggccttcttc aatcgacatg ctgtgcgaaa
    29041 gcggcgacat cagcgaacag gagaggtatt gcgaaacacc gtccagttcg ccaacggtaa
    29101 aggtcatggt gccatacggc agttgcagac caattttttc actcactttg cgcagcggcc
    29161 agagttgatc cgggccgggg aagataactg cactcagcat ccacggggtg atcacacacc
    29221 ccgtccactg accttcgaac agcgtaaaat cagaaacata caccggcatt gaaggatgca
    29281 gaaaagagag atcgtgcatc gaacgccggg caatttcttc aaacgctgcc tgtacttgcg
    29341 ccttcgggga ggtctggaaa cctgctatct cttcagtcat gaatcgcctc ccgtgggatg
    29401 gcttccacgc ctgattcgcg cagcgcagcc agaacctgct caagcgcagg ttcaatcatt
    29461 gcttcaaccg tcggcgttaa gccgatgtgt ggctccagcg attccgggat cacgccgacc
    29521 agggtcagct ttttcggaaa ctcaccggtg aagcgcaggg ccgacaatac gtcggccagg
    29581 ccaagctgat gcggagagat tttgttggta aacaacgccg gaacttcttc atcccgcagg
    29641 atcatcatcg ttcccggagt gttctttttc gacacaatgg catccgcaat aatcagatga
    29701 tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg tgccgccatc gaggatctca
    29761 acataatccg gcagaatgta tcgttgctct aacgcttcga caatccgcac gccgatggct
    29821 tcatcggtca gcaaaatatt gccgacccct aagactaaaa tacgcattac agaaccttca
    29881 ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac cgcacaggcc atgcacgggt
    29941 cgaaggagtg aatggtacgc accacttcca gcggtttatt cggatcggca accggtgtac
    30001 ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa gttacgcgga ccagagttcc
    30061 aggttgatgg aacaaccgcc tggtagttgc tgatgatacc gtctttaata accatccagt
    30121 gagagagcat accgcgcggc gcttcgagga agccaacacc tttgaactca cccgttgccg
    30181 gaatgttcgg cttcacaaag gtggtgtgat cgcctttgcc gatattggtg atcagtgcac
    30241 tgtattggtt ttgcaggata tcctgcaatt cacagcagtg aacggtacga ccaataatac
    30301 ggcccagcgt ggagtgcagc tgtgccactt ccagcgtgtt gccagtcagt ttctgataaa
    30361 tcgcaacgat ttcattcagt ttgttctggg tagattcgcg acctgccgcc agtttcacca
    30421 gcatattagc cagcggaccc acttctaccg ttttgccgta gaaagtcggt gatttcaccc
    30481 aggaatattt cccgtcgtca gaccaaccat cataagccgg aatggtggta ccttcccacg
    30541 gcgcctgtgg cgcttcgtct ttataccagg agtgcttcgc gctttcctga atgcctttga
    30601 tcaggtattc atcggaatga gaagtgatcg gacgatacga ggacagatcc gcattctcaa
    30661 tgtagcctcc cgggaacagg aagctgccgt ttttgctgtc ggtcgggaat tccggcacgc
    30721 tcaggtagtt caccgcacct ttaccgcgcg tcagccattc cgggtagaac gcggcgataa
    30781 ccgcggtatc aaccttataa acctgctcaa caaagtcgct cagtttgtcg atgaaagact
    30841 tgatgtacat caggcgctca aggttcaaca cgcccaaccc gtcgaggttg attgggttcg
    30901 cgacaccacc cacagccagg ttctgaatgt gcggcgtttt accgcccagc agcgccacga
    30961 cgcggttagc gtcacgctgg cactccagcg cctgcaggta gtgcgctacc gcaatcaggt
    31021 tcacttccgg cggcagtttc atcgccgggt gaccccagta gccattggcg aaaataccca
    31081 actgaccgct ggcaaccaga tctttgatct tgttctgaac tttagtgaac tcttccggac
    31141 tgttcagatg ccaggtcgaa acgcctttca gcatttcgga ggctttggtt gggtcagctt
    31201 gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa tgaacgatat
    31261 ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat gtattgcgcg ttaaccggaa
    31321 cgtcgatatt cagcgcactt tctgccgcac gaacggaaga cagcgcgtga gtggtagtac
    31381 atacgccaca gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg tttttcacga
    31441 tctcttccat gccgcgccac atggtaccgg aagcccatgc tttcgaaacg acgccatttt
    31501 cgatttcgca atcgatgcgt aaatgcccct caatacgggt taccggatca atagtaattc
    31561 tctggctcat gctttgctcg cctcatgacg attatgatcg ttttgtttta aaggaggaag
    31621 tatcggcagt agacgaatga gtacgatgta agcgcaaatc tcaatagcca caaaaccaat
    31681 agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc
    31741 caccaacgaa taggtcagac gccaggttgc acaacctaac agtgcgctca gtgctgacag
    31801 gaacagcata cgggaatcat tacgcagctt cgccacacgc agaacgacca gcgggaagag
    31861 catcagcagg acttcaatcc agaacatcac ggagtagaag tcaccggcaa acgctaacga
    31921 cagcttgtcg cgatagataa gctcgccaaa gcgcagcccg atgaaaatcg ccagcaacac
    31981 actgatggtg ttggtcagct tgacgaacag gctcttttca tccggaccgt tgccacgcag
    32041 acccgcctgc accagtgaac cttcaaagat gacaatcgag aagcccatga tgaacgccgt
    32101 cagcagcgag aacagcggca acatttcata gctctgccag agcggatgca ccttgtagcc
    32161 cgccgagatc atcagcgacc ccattgaaga ctggtgcatg gtcggcagca gcgcaccgag
    32221 cgcgatgatg aagaacatca ccttgttcag acgttgtagc gacaccttcc agcccagacg
    32281 ttcaaacagt gccggagcaa actccagagc catcacgccg atgtagatgg tcatacagac
    32341 cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga agtacggcag
    32401 gttccagtag cgacccacgt cgatagtgat cgacaagcca cccagtgagt aaccaaacag
    32461 actcgccaac agcgccggac gcaccagcgg atggtattgc ccacggttaa agacgtatac
    32521 cgcccacgcc agcgcccagc cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc
    32581 gatccacacg ccccacggga agccgccgtt caggtcagag acagagccca gaccgaacac
    32641 cagacgcttc acaatcagga gcatacagat gacgattaac ggtccaaaaa tcatgaccgg
    32701 tttactgatg attttgccgc ccagcggttg tggatcatga ctcatgatcg tctcctccgt
    32761 cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt taagcccgcc agcacagcca
    32821 gtggtagcat catgccttta tacagggtgt gttgaatatt ttcggaacgc gcaccggtag
    32881 aaagatcgtc cagtttcggc aggtcgagat tttcataagg cacacccgtc agtaccagaa
    32941 cctgagtacc gccgccctct ttctcgccgt acagatgcgg ataatatttc ggcaccgtat
    33001 gcaggtaagt gtcgccagat ttcagcgtct gacgcggata gtggtattcg ctgccaggct
    33061 tcagcgccag acgttttttc gcctccgcca tcagctcttc acgcgtaccg aaaatcaccg
    33121 cgcccgccgg gcacacttct acgcagccag gcagaccgcc tttatcgaga cgttccacac
    33181 ctttctggtt gcacagctcg cacttatgca gcgcaccaaa cgggttgttg tagtcgtact
    33241 tcggcacgtt gtacggacag gcgaccatgc agtaacggca accggtgcac acgtctttgt
    33301 cgtaatggac aatgccggtt ttcggatctt ttttcagtgc agagaccggg cacacagaga
    33361 cacagttcgg atcgacgcag tgcatacact gtttcttaat gtacgcgtag ccgttctcct
    33421 cctggtcttt gttgacccct gtgccgctgg tccacacctg aatgatgtta ttggtatacg
    33481 gcgacagttt gtcgttgttc gaccaggtct gttccccttg cgggttacgt tcagggaagt
    33541 tgatatcctg acacttggtg acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca
    33601 acatccccag cgatcccgga attggcgggc ggttttcagc agccgcatga ctgacagacg
    33661 gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt aataaaatta cgtctgttca
    33721 cggttattct ccccgtgagt cagcgttatc tttcttttgc tgacgaccca gttcacgcac
    33781 cgccatcacg ctgacaccgg caaccagccc aaccacaccg ccgagcaaac caatagcgcc
    33841 tgcagagacg ttgccgccct ctttagcgtt aacatccggt ttctgtgaac gcggagtctg
    33901 attttcgacg ttggcaagct gatggatgcc tttatggaag ccgatacctt tttcgttaca
    33961 gccatagcac gggtgaccaa tcgccaccgg ccacacaccg ccaacatcgc agaattgcag
    34021 cgttgagcag ttgccgtaag tttctggccc tttacagccg aggtggtaca agcaccagcc
    34081 ttcgcggtgg ccttcatcac cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg
    34141 acgttcgcag tgttcgtgaa tcagacggcc ataggcgaag gtcggacggt ttttgtcatc
    34201 cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg
    34261 gttcggcggg cagcccggaa tattgataac ggttttgcct ggcagaactt cttgcaagct
    34321 gactgcgcca gttgggttaa ctccagctgc ggcaacaccg ccccacgcgg agcaggaacc
    34381 aatagcgata atggctgctg agccttccgc cgctttgcgg atgtgatcca caatcggctc
    34441 accggcaacc atgcaataaa taccgttatc ttttaatggg atggaaccat ccaccactaa
    34501 cacatactgc cctttgtact tctcgagagc gttatgtttg ttctcttcga cctgatgacc
    34561 gaaggcggcg gaaagcactt cgtgatattc cagagagata gtctccagca cgaggttttc
    34621 taccgttgga tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc
    34681 aatccagata actggcggac gctgcgggtt agtaaccgat tcggccatct ctgcagcggc
    34741 tttgctactt aaccccatgg tggcggctaa tgctgcacaa agcttcatga aatcacgacg
    34801 gttaatgccg tgagaatgga tgagggtgtt atctccagtc atttatagtt attccgttgc
    34861 gagaacctgg catatatttt gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt
    34921 taccacacat ttattatggt tatcgatatc atgatactgc ggcggggcgc taaaacgaag
    34981 ggaagaagca ttagtgtaat taattaaagc aagataatac gtatgtttga tcaattttcg
    35041 aggcgataac cggccataaa aaagcgcaac gtagaacagg aattattctc acgaaagaaa
    35101 tccattacca tctctttatc cagtccatag cggcgcgtca gaatacccgc ttcccaggcg
    35161 ctaagctggc gatctccggt cttttcgtac atcctgtgtg agtagcctaa tacaaaaccg
    35221 cgtttataat cggcacagaa ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt
    35281 aatcccgcca ttaagccttt accaaaatgg ttattcatcg ttcacctcat tcattatata
    35341 tcgccatata taacgatatt ttaggcaaag aacgactttt gcgcacgcta ttttttcata
    35401 cttgtttaag acgtaatccc gtaagagata aacctatcag gagagatatt tatcgaacca
    35461 tttcagtgtc ctttgccagg caagatcggc ggcagatttg tcataacggg gcgtggaatc
    35521 attatggaat ccgtgattaa cccccggata gatataagcc tcataaacct tattattgtc
    35581 tttcaacgcc gcctcgtaag gaggccagcc ctcgttgatt cgggtgtcca gttccgcgaa
    35641 gtggagtagt aaaggcgctt caatcttcgc cacatcggca gtgggtgcct gacgaccata
    35701 aaacggcacc gcgcaggcca gttccggata cgcgacagcc gccgcgttcg atacgccacc
    35761 gccatagcaa aatccggtaa tacccacttt acctgtcgct tgcggatagc gttgcataaa
    35821 ctcaattgcg gcaaagaaat cattcatcag tttggttgga tcaacctgct gttgcagctc
    35881 acgaccttta tcatcatttc ccggataacc tccaacggaa cttaatccgt caggtgccag
    35941 ggcgatatac cccgccttcg ccactcgccg tgccacatct tcgatatacg gattcagtcc
    36001 acgattctca tgcaccaccc ccacggctgg cgttttgccg ctcatttttg cgggtttcac
    36061 cagataaccc cgtacctcgc cgtgaccatt tggcgaagga tacgtgatgt actcagcaac
    36121 aatctccggg tcggtaaact ctacctgagt cgccagcgca taatttggct tgagcaaatc
    36181 aaacaacgct aatgccgtca tcccgcccac cgcatacttc gccgcaaggt tgaggaactc
    36241 acgtttcgaa attttcccgt gaacgtaata gtcgtagtaa tccaacaact cttgtgggaa
    36301 atctttggcg gttaaacgcg gcatcgttgc attcctcaac tggtgttttt ttaagcaaag
    36361 cataagcgcg tgtttttgcc cagtttttcg tcactctgtg agccagacta cgggatacgc
    36421 gctggcgaat cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc
    36481 atggtctggt tagcgaatcc cgaacgttac gggcagatgc agtaccgcta ttgcggaaaa
    36541 agtggtttac gcctgcccgc attatcgctc ggtttatggc acaatttcgg tcacgttaac
    36601 gcgctggaat cacagcgtgc aatcctgcgt aaagcgtttg atttaggcat tacgcacttt
    36661 gatttagcca acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg
    36721 ctgcgcgagg attttgctgc ttatcgcgat gaactgatta tctctaccaa ggctggctac
    36781 gatatgtggc ccggcccgta cggctctggc ggttcacgta aatacctgct cgccagcctc
    36841 gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc
    36901 gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt
    36961 aaggcgctgt atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag
    37021 ttgctgcacg agtggaaaat tccgctttta attcatcaac cttcgtacaa tttactgaac
    37081 cgctgggtgg ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgtatt
    37141 gcctttactc ctctggctca gggattgctg accggaaaat atctcaacgg cattccgcaa
    37201 gattcacgga tgcatcgtga agggaataaa gttcgtggtc tgacgccgaa aatgcttacc
    37261 gaagccaacc tcaacagcct gcgcttattg aatgaaatgg cacagcagcg tggacaatca
    37321 atggcgcaaa tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt
    37381 ggtgccagcc gcgcggagca actagaggag aacgtgcagg cgctgaataa tctgacattt
    37441 agcaccgagg agctggcgca gattgatcag catatcgccg atggcgagct gaatctgtgg
    37501 caggcgtcgt ccgataaatg acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga
    37561 tcagtgatta tgacgagtca gtcggtcaag atagcccatc acaaatgcag aaagcacaaa
    37621 cgtcagatgg ataatgacgt accacatcag tttgttatcc gggacatttt tcgcatccat
    37681 aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt
    37741 cagcgacgtt gcgtccattt tccccagcca cttcagcttc tctttgttct cggagatatc
    37801 cagctgtgag acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac
    37861 cagcgtcata tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa
    37921 gatattcggc agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc
    37981 cagcgaaagg ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc
    38041 aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt
    38101 gagtaaacag cacgcaacag caaatcaaca tatacgttaa acctttgtcg cataattctg
    38161 aggtttaggg atcttcctgg ttcccttatc aacgcatcga gagcgtctac ccttaaagga
    38221 cgttgaacct gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta
    38281 cactggtgaa tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc
    38341 gataccggat tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc
    38401 actggtgaca gggggcgatt ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga
    38461 aggggctgac gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa
    38521 aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga
    38581 gaaattcgcc cgttcgctgg ttcacgaagc gcacaaggcg ttaggcgggc tggatattat
    38641 ggcgctggtc gccgggaaac aggttgccat tccggatatt gcagacctca ccagcgaaca
    38701 gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat
    38761 ccccctgcta ccgaaaggtg caagtgtcat caccacttcg tcaatccagg cataccagcc
    38821 aagtccgcat ttactggact atgcggctac gaaggcggcg attctgaact acagccgtgg
    38881 cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggccctat
    38941 ctggacggca ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca
    39001 gaaaacgccg atgaagcgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct
    39061 ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca
    39121 tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct
    39181 tacctgatgt ggcgaaaacg ccttatccgc ctacaaacgc atgaagattc aacatgttgc
    39241 aatagatttt gtaggcctga taagcgtagc gcatcaggca ttgagcacct aatgccggat
    39301 gcgtcgtgaa cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat
    39361 tttgtaggcc tgatgagcgt agcgcatcag gcaattctac tttacttcgc tttggcggtt
    39421 tcttcgccga ccagacctat cttcaggtaa cccgcctgat gcagcgtatc cattaccttc
    39481 atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga agatggtggt gtctttcttg
    39541 ccttcggtta acgcattcaa cgccgtaatc attgtttcat cggtgaccgg atcgttaccg
    39601 ataaacatcg agttgtctgc cttcaccgac agataaaccg gtttttccgg gcgcggctgc
    39661 ggcgtgctgg tagaagcagg caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc
    39721 accataaaga taatcagcag aaccaacatc acgtcgataa acggcgtcac gttgatatca
    39781 tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg acattatcct
    39841 gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc cagatctagg
    39901 tcacggcttt gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc tttaaagccg
    39961 ccaatctggc gtgcaaatac gttatagata acgaccgcag gaatcgctgc cacgaggccg
    40021 attgccgttg ctaacagagc ttctgcgata cccggcgcaa cgactgccag gttagtggtc
    40081 tgcgtttgcg cgataccaat aaagctgttc atgatgcccc agaccgtacc aaacagacca
    40141 acgaacggag aaatcgcgcc gatggttgcc agatagccgt taccgcggcc catttgacga
    40201 cccactgcgg cgacccgacg ctccagacgg aagctggtac gttctttaat accttcgtta
    40261 tcgtcgctgc cttctgacag ttccagctca ttctgcgctt cattgagcaa atgcaggctt
    40321 aagcttttgc taccaaaatc agcggcgata tcgttggcct ggtttaagga acgtgcttca
    40381 gccagcagtt gctgctcgcg cttaaggcga cgcttctgat tgaagaactc tacgctctta
    40441 ctgaagaaga ttgcccaggt gactacagag gccaaaataa gcccaatcat cacgcactta
    40501 acgacaatat cggcgtactg atacataccc cagacggaaa ggtccgtctg cattaaatta
    40561 ttacccactg tgtatctcca ggacgcaagt cacaaaatct gcgcataata atatcaaaac
    40621 gacgtcgaat tgatagtcgt tctcattact atttgcatac tgccgtacct ttgctttctt
    40681 ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt
    40741 tatgccaatt cttcaggatg cgcccgcgaa tattcatgct agtttagaca tccagacgta
    40801 taaaaacagg aatcccgaca tggcggacaa aaagcttgat actcaactgg tgaatgcagg
    40861 acgcagcaaa aaatacactc ttggcgcggt aaatagcgtg attcagcgcg cttcttcgct
    40921 ggtatttgag agtatggaag ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt
    40981 gttctatgga cggcgcggaa cgttaaccca tttttcctta caacaagcga tgtgtgaact
    41041 ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg gcagcggttg ctaattcgat
    41101 tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct atgaaccgag
    41161 tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt ggtttgatcc
    41221 gctgattggt gccgatatcg ttaagcatct gcagccaaac actaaaatcg tgtttctgga
    41281 atcgccgggt tccatcacta tggaagtcca cgacgttccg gcgattgttg ccgccgtgcg
    41341 cagtgtggtg ccggatgcca tcattatgat cgacaacacc tgggcagccg gtgtgctgtt
    41401 taaggcgctg gattttggca tcgatgtttc tattcaggcc gccaccaaat atctggttgg
    41461 gcattcagat gcgatgattg gcactgccgt gtgcaatgcc cgttgctggg agcagctacg
    41521 ggaaaatgcc tatctgatgg gccagatggt cgatgccgat accgcctata taaccagccg
    41581 tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc
    41641 cgaatggctg gcagaacatc cgcaagttgc gcgagttaac caccctgccc tgcctggcag
    41701 taaaggacac gaattctgga aacgagactt tacaggcagc agcgggctat tttcctttgt
    41761 gcttaagaaa aaactcaatg atgaagagct ggcgaactat ctggataact tcagtttatt
    41821 cagcatggcc tactcgtggg gcgggtatga atcgttgatc ctggcaaatc aaccagaaca
    41881 tatcgccgcc attcgcccac aaggcgagat cgattttagc gggaccttga ttcgcctgca
    41941 tattggtctg gaagatgtcg acgatctgat tgccgatctg gacgccggtt ttgcacggat
    42001 tgtataacat tgccactttt ggacaatttt gcagacattt tattgtgaaa agtcttaaat
    42061 tgttgcgtcc gggatcaagg cgtcccggac gattcaggag tacaataggc agataaaggc
    42121 ttaaacgctg ttccacagga aagtccatgg ctgttattca agatatcatc gctgcgctct
    42181 ggcaacacga ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt tactttgtca
    42241 tgtttgccac gctgttttta gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca
    42301 gcttgttgat actggcaggc gcattgattg cccaaggggt tatggatttt ctgcctacga
    42361 ttgcgattct gaccgccgca gcaagtctgg gctgctggct aagttatatt caggggagct
    42421 ggttagggaa taccaaaacg gtgaaaggct ggctggcaca gcttcctgct aaatatcacc
    42481 agcgcgccac ctgcatgttt gaccgccacg gtctgctggc gctgctggct ggacgttttc
    42541 ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc
    42601 gcttccagtt tttcaactgg ttaagtggat tgctgtgggt cagcgtggta accagttttg
    42661 gctatgcctt aagtatgatt ccgttcgtta aacgccatga agatcaggta atgacgttcc
    42721 tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg tttgtggtga
    42781 ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt cccggctgct gtaccgggaa
    42841 cgtatttaat tcccctgcat cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg
    42901 aagtaacgtt taaactcacg gctaaattgc gatgcgcttt catagccgac gcgcatcgct
    42961 gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc gcgccttatg cagacggtaa
    43021 ttcttcaaat actgcaacgg cgaggtgctg gtgacagact taaaattatg gtggaacgcc
    43081 gatacgctca tgttggcttc tgccgccagt tgctcgacgc tcaggttttc ggtgtattta
    43141 ttctcaattc gtttcagcac gcggctaatc agactgaagt gagtctggcg actgaccagc
    43201 gccagtaacg cgccgccgca aggtccggtc agcacgtagt acagaatttc gcggatgatc
    43261 tgtttgccga gaatacgcgc atccagtggt cgctccatca cgtcgagtaa ccgctccgcc
    43321 gcgcataaaa tctcttctga taacgtggcg gagttaatcc cgctggctgc catcgacggc
    43381 tggaaatgct catcttcgcc aatgtccatc aacagttcct gtaactgcaa aatatcgaca
    43441 ttgagacgca accctgccag cggcacctct gacgtggcat aggtttcgca ctcaaacggc
    43501 aacggcaccg tcagcagcag gtattcattg gcatcataac gaaacacgcg ttcattgata
    43561 taaccgattt tatgcccgga aaagagaatt atgatgccag gctcgtacat caccggtgta
    43621 cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg gcaacagttc tgacaaacta
    43681 ttttctttat ttttcagttt attaacttta tccgccagca agcgacaaat ctcttcacgt
    43741 ttcatatcgc gtaatttctt aggaataatg cggcaatttg attgtgcgca attttgtagc
    43801 atttctccag cactctggag aaataggcaa gacattggca gaaatgagca ttgagagcca
    43861 gggcgctggc gatcacaatg aaaaacatca ggcagatcgt tctctgccct catattggcc
    43921 cagcaaaggg agcaagtaat gaacaacttt aatctgcaca ccccaacccg cattctgttt
    43981 ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc acgatgctcg cgtattgatt
    44041 acctacggtg gcggcagcgt gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg
    44101 aaaggcatgg acgtgctgga atttggcggt attgagccaa acccggctta tgaaacgctg
    44161 atgaacgccg tgaaactggt tcgcgaacag aaagtgactt tcctgctggc ggttggcggc
    44221 ggttctgtac tggacggcac caaatttatc gccgcagcgg ctaactatcc ggaaaatatc
    44281 gatccgtggc acattctgca aacgggcggt aaagagatta aaagcgccat cccgatgggc
    44341 tgtgtgctga cgctgccagc aaccggttca gaatccaacg caggcgcggt gatctcccgt
    44401 aaaaccacag gcgacaagca ggcgttccat tctgcacatg ttcagccggt atttgccgtg
    44461 ctcgatccgg tttataccta caccctgccg ccgcgtcagg tggctaacgg cgtagtggac
    44521 gcctttgtac acaccgtgga acagtatgtt accaaaccgg ttgatgccaa aattcaggac
    44581 cgtttcgcag aaggcatttt gctgacgcta atcgaagatg gtccgaaagc cctgaaagag
    44641 ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc gctgaacggt
    44701 ttgattggca ctggcgtacc gcaggactgg gcaacgcata tgctgggcca cgaactgact
    44761 gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat
    44821 gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc
    44881 actgaaggtt ccgacgatga gcgtattgac gccgcgattg ccgcaacccg caatttcttt
    44941 gagcaattag gcgtgccgac ccacctctcc gactacggtc tggacggcag ctccatcccg
    45001 gctttgctga aaaaactgga agagcacggc atgacccaac tgggcgaaaa tcatgacatt
    45061 acgttggatg tcagccgccg tatatacgaa gccgcccgct aagcttttta cgcctcaaac
    45121 tttcgttttc gggcatttcg tccagactta agttcacaac acctcaccgg agcctgctcc
    45181 ggtgagttca tataaaggag gaacgtatgg ctaatccaac cgttattaag ctacaggatg
    45241 gcaatgtcat gccccagctg ggactgggcg tctggcaagc aagtaatgag gaagtaatca
    45301 ccgccattca aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc gcggcctaca
    45361 agaacgaaga aggtgtcggc aaagccctga aaaatgcctc agtcaacaga gaagaactgt
    45421 tcatcaccac taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc ctgctcgaca
    45481 gcctgaaaaa actccagctt gattatatcg acctctactt aatgcactgg cccgttcccg
    45541 ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa gagggattaa
    45601 tcaaaagcat cggcgtgtgc aacttccaga tccatcacct gcaacgcctg attgatgaaa
    45661 ctggcgtgac gcctgtgata aaccagatcg aacttcatcc gctgatgcaa caacgccagc
    45721 tacacgcttg gaacgcgaca cacaaaatcc agaccgaatc ctggagccca ttagcgcaag
    45781 gagggaaagg cgttttcgat cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa
    45841 ccccggcgca gattgttatc cgctggcatc tggatagcgg cctggtggtg atcccgaaat
    45901 cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg ggatttccgt ctcgacaaag
    45961 acgaactcgg caaaattgca aaactcgatc agggcaagcg tctcggtccc gatcctgacc
    46021 agttcggcgg ctaacgtgca aattctcccg gtggcggtaa tgttccgcta ccggactttt
    46081 cagaaatcat ttattcccct cgcgtcccgc ccgttgttac tcttccttgt tcaggaatgc
    46141 caaatataag gacatcatca tgcagagccg gaagctctta aaagaacaac tcatctatat
    46201 ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa taatacttct gtttttagca
    46261 gccctggcaa gttttaccgt acacgcgcag cctccttcac agaccgtaga acaaacagtc
    46321 cggcagattt atcagaacta taaatcagat gccagtacgc cttatttcgg tgaaaccgga
    46381 gagcgggcga taacttcagc gcgtattcaa caggcgctta ccctgaacga caatcttaca
    46441 ctaccgggca atattggctg gctggattat gatccggttt gtgattgtca ggattttggc
    46501 gatctggtgt tagaaagcgt agcgataacc caaactgacg tcgatcatgc cgatgccgtt
    46561 gtgcgctttc gtatctttaa agatgataaa gaaaagacca cacagacact gaaaatggtg
    46621 gcggaaaatg gtcgttgggt cattgatgat attgtcagca atcatggcag cgtcttacaa
    46681 gcagttaata gcgagaatga aaaaacgctg gccgctttag cttcgttgca gaaagaacag
    46741 ccagaatcct ttgttgctga actctttgaa catattgctg attatagctg gccgtggacg
    46801 tgggtggttt ccgactctta ccgccaggcg gttaatgcct tctataaaac caccttcaag
    46861 acggccaata atcccgatga agatatgcaa atagaacggc aatttattta cgacaatccg
    46921 atctgttttg gcgaagagtc gctattttca cgcgttgatg aaattcgagt cctggagaaa
    46981 accgccgatt ccgcccgcat tcatgttcgt tttacgctga ccaatggcaa caatgaagag
    47041 caagagctgg ttttacagcg gcgcgaaggt aagtgggaaa tcgctgattt tatccgcccg
    47101 aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt aaagcaatga
    47161 gctgaattaa ataacaatta gccggaacaa taaataaaag ggaacactat atgaaaacga
    47221 ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc gtcaatgagc
    47281 aaaaggtcaa tcaactggcg agcaatgtac aaacattaaa tgccaaaatc gcccggcttg
    47341 agcaggatat gaaagcacta cgcccacaaa tctatgctgc caaatccgaa gctaacagag
    47401 ccaatacgcg tcttgatgca caggactatt ttgattgcct gcgctgcttg cgtatgtacg
    47461 cagaatgata aaaaaatccc cggcagcatg tcagttgccg gggatttttt ttaacgtcca
    47521 accgccgctt tagggcgttt cttcgcacca gcattcaccg gacgagattg cgtagacgac
    47581 gctttttttg ccgtagcagg cgtctgacgc tgagtcgcca tcggcgtatg tttagtcaac
    47641 gccggacggg tattgcggtt ctggcgacga gcttcacgca tctcttcaat ggttggcgca
    47701 ggcactaagc aatcgcgacg gctgccaatc agatgctttt tgcccatcgc ttccagcgcc
    47761 tggcggatta acggccagtt tgccggatcg tggtaacgca acaacgcttt atgcaaacga
    47821 cgctgtttgt cgcccttcgg tacgaagacg ccttcgctct tataaccaat cttcgccagc
    47881 ggttttttcc cggtgtaata catggtggtt gagttcgcca gcggcgatgg gtagaagttc
    47941 tgtacctggt cgagacggaa acgatgcttt ttcagccaca gcgccaaatt caccatatct
    48001 tcatcacgcg taccggggtg cgcagagatg aaatacggga tcagatactg ctctttacct
    48061 gcctgtttcg agtaggtatc gaacagctct ttaaagcggt catagctgcc catgcccggc
    48121 ttcatcatct tcgacaacgg cccttcttcg gtatgttccg gagcaatctt cagataaccg
    48181 ccgacgtgat gggtcgccaa ctctttgata tagcgcggat cttccacggc gatgtcataa
    48241 cgtacgcctg aggcgataag gattttttta atgcctttca gatcacgcgc acggcgatag
    48301 aggttgatcg tcggttcgtg gttagtgtcc atgtgcggac aaatatccgg ataaacgcac
    48361 gacaaacggc gacaagtttg ttcagcgcgt ggcgatttgc agcgcaacat atacatgttg
    48421 gcagttggcc caccgagatc ggaaatcacg cccgtaaaac ctggaacggt gtcgcggatc
    48481 gcttcgatct cattaatgat cgaatcttcc gaacggctct gaataatgcg cccttcgtgc
    48541 tcggtaatag aacagaaaga acagccgcca aagcagccgc gcataatgtt gaccgaaaaa
    48601 cggatcattt cgtaagccgg aatacgggca ttgccatagg ccggatgtgg cacgcgcttg
    48661 tacggcagcg caaaaacgct gtccatctct tcggtagaaa gcgggatagc aggcgggttg
    48721 atccacacat agcggtcacc gtgtttttgc atcaatgcgc gggcgcagcc tgggttggtt
    48781 tcgtggtgca gaatgcgcga agcatgggcg tacagcactt tatcgccctt cactttctcg
    48841 aaagaaggca gcaatacgta ggttttttcc cacggtttct ggcgcggtgg ctgcacgatt
    48901 acagctttcg cttcctgctt tttcggcgca acaggtttgt tatccgcaca cggcaaatct
    48961 tcgccatacg gatgcgggat tgggtcgatt ttccctaggg tatcaagacg ggtggaatcc
    49021 acaccgctcc agccaggcag cgcctctttt acgataattg cggtattacg cacatcgcgg
    49081 atttcactaa tcggctcgcc catcgccaga cgatgcgcta cttccaccag cggacgttca
    49141 ccgttaccaa acatcagcat gtcggctttc gaatctacca gaacggaacg gcgcacggta
    49201 tcggaccagt aatcataatg cgcggtacgg cgcaggctag cctcaatacc gccgaggatc
    49261 accggcacat ctttccacgc ctctttacaa cgctgggtat aaaccagcgt ggcgcgatcc
    49321 ggtcgcttac ccgcgacatt atctggcgtg taggcatcat catgacgtaa acggcgatcg
    49381 gcggtatagc ggttgatcat cgaatccatg ttgccagcag taacaccaaa aaacagattc
    49441 ggtttaccca gacgcataaa gtcgtctttg ctgctccagt ccggctgggc gatgatcccg
    49501 acgcgaaagc cctgcgcttc cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga
    49561 tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc
    49621 atctcttcac gagacatcgg caaaaacggt gccggtccaa aacaggcggc ccagtactgc
    49681 ggccaggaga acaggtcgcg atccggttgg atcagggaga tagagctcat tttgcttcca
    49741 gaaatgataa aaaaataatc aaaggccggg gattataagc cggaacgaaa gagaaatcga
    49801 aaggtattcc atactcgccc tcctcgggcg agtatgaaga ttacggtacc ggattgacca
    49861 acagttgccc aatcgagcca cggtccgcca tttccagcgt ctgactgttg aagtagaacg
    49921 ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc
    49981 aaacggtatc tttccagcct ctgtcttccg ggaacggcat cgcgccattc acgttacgga
    50041 tctggaacat tacgccttca atatggaacg cttgcggctc gtccgcgcgt accgtccagc
    50101 gttcccacgt tccttgctgc gcggtgacat caatacggtt gacgtcccac agctgtccat
    50161 taatacccgg gtcatcaccc agactgatat cgcgactgcg aattggcgaa ccagccatga
    50221 tttcagttgg cagcaagcgc atcggaagac tgtctgtgac cagcggcaga aggccggttg
    50281 ggcgcagcgt tagcaccaag gtagaaacca gaatactgga tggctcaaag aagccgcgaa
    50341 tacgatcaac aatgctcgcc gcttcaccac aggtgatcga cacttcatcg ccgttgctca
    50401 tatccaccag aatctcgcgg cgctcgcccg gtgccagcga aagttgcttc accgatacag
    50461 gagcaggcag gaatccctga tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct
    50521 gtagttgata gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc cagccacgcg
    50581 agacttcaac gtacgggctt tgtacaccgt taaccagcag cgtatcacca acaaagccgc
    50641 cgcttcccgg ttcgttgtat tctggcgtac caaagttatc cagccgttta tcctggataa
    50701 tgaccggaaa atcatccaca ccataatggt tggggatagg cagcgacttg ctgacttcat
    50761 cttccaccag ccacattccg gcaaggccgt tatagacctg ctgagccgtg cggttgggag
    50821 tattggcgtg ataccacaga gtagctgcgt tctggcgaat aggcagtacg ggtgcccagt
    50881 cagcgtttgg cgacatcatc cgtgccggac cgcccatcag cgggcctggt acctgtagcc
    50941 cggccaccgt cattgagaca ttttctgtca ggcggttgct gtaaataagc ttaacatcgt
    51001 cgcccttcca gacgcggata gtcggcccca ggtaacgacc attgattccc cagaccgacg
    51061 cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac agtcataaac agcggttgcc
    51121 cacggcgaga ttcaagtagc ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg
    51181 ccttcagggg aacagcgcct gcacaaagtg caatccccga tgcctgaatg aactgacgcc
    51241 gactgagtga catatttgct ccatgtaaaa ctggctaaag taataccgtg caggaaagtg
    51301 ttccccgcaa aaatacagat tgccgactta aacttttccg gcggcttcgc gttctgcgac
    51361 ttctttatcg agctcggcga ttttttgttc cattatcgaa cgacaatggg cagccagctc
    51421 acgaacctga tctttgccat actgactgac gtcaattggc ggcagcattt cgacaatcac
    51481 cagaccgttg tgcagtcgat taagattaat cttattcgaa gttgtagaga cgcacacggg
    51541 aataatcggg acgcccgccg caattgccgc gtgaaatgct ccagtcttga acggtagcag
    51601 gccgcgacca cggctgcggg ttccttccgg gaacatccag atggaaatac ggcgtttttt
    51661 gaaatgattc acgacttccg caatggtgcc gtgagcttta gtgcgattgt ttctgtcgat
    51721 caataagttg ccggttaacc agtacaactg cccgaagaag gggatccaca gcaagctctt
    51781 tttacctacc gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca tgtcatagtt
    51841 gttctggtgg ttagcgatat agatagcatt gccgtagctt tcagcatctg caggtttgcg
    51901 gcactcaact ttcaggccga acagtggcgc aagacggcca aacatatgcc caaaggtggc
    51961 cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg gagccgaata cacagactaa
    52021 gatgctgtaa atcacggtaa taataagacg aaagatatat agcataacac cctctgaagg
    52081 tctgagagac tggcattgta cgtcacctga aatcaggtta gggcattgtt aaaagccgct
    52141 ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa caacggtttc aagggaattt
    52201 ttatcgtttc cgggggttga atacgctgcc ggatgacgac ttaacgtttc atccggcgtt
    52261 ccttgctagc gggaggaaac agcgccctcc ccggcatatt actcttcgct atcaccgctg
    52321 ctggcacggc gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac
    52381 gtaccgcggc gtccacgttc gccagtgact ttctgtagct cttccggacg cagtttaatt
    52441 ttgcgtttcc caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta cagttgcgcc
    52501 agaccatcct cgccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg
    52561 cccttcgaca gctgcggcag atcgcttacc gggaacatca acatacggcc tgcctgagtg
    52621 attgccagca gcatatcgga agcatcttca atcaccaccg gcggcataac atgggcattt
    52681 tccggtaagg tgatcaaagc cttacccgca cggttacgcg ctaccagatc gttaaaggtg
    52741 cagacgaaac cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc
    52801 agcatatggt caacggtcgc cccaggcggc aacgttaatt tgccggtgag tggttcgccc
    52861 tgaccacgcg ccgacggcag cgtaatcggg tcgatggcat agctacgacc ggtggaatca
    52921 acaaacacta ccggttggtt actcttacct ttcaccgccg ctttgaagct atcacccgct
    52981 ttataattca ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc
    53041 tgcgacagca caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc
    53101 gcttcttcgc gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc
    53161 tgcagttctt tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac
    53221 tggtcgcgct ctttttccag ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg
    53281 gcaagatgac gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac
    53341 cgcgacatca gcgccggttt cggttcatct tcgttacgaa tgatctcaat cacttcgtcg
    53401 atgttgagaa acgccaccag caaaccttcc aggatatgca ggcgcttgag gactttctcc
    53461 agacgatagt tcagtcggcg gcgcacggta tcgcgacgga acaccagcca ttcggagagg
    53521 atttccagca ggtttttcac cgccggacga ccatccagac cgatcatatt gaggttaatg
    53581 cgatagctct tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc
    53641 acgcggttgg aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg
    53701 cgcagatcgt caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt
    53761 acgcgcgcac ctgaaacctg atgcggtaat gcgctgataa ccaccgcgcc atcttctttc
    53821 ttccacaccg cgcgcatacg cactgaacca cgtccgttct cgtagatttt acgaatctcg
    53881 gcgcgcgaag tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc
    53941 agctgatcga gcgtagtttt cggctggtcg attaatgcga ttgccgcctg agctacttca
    54001 cgcaggttat gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta
    54061 agcaaaatgt ttggcagacg ggcaggtagc attttcggct cctgcaaagt gccgtcgaag
    54121 tttggcaccc agtcagccgt cccctgcccc agctcgctca atagcagctc ggaatatttc
    54181 gacaaccggg attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc
    54241 cagttcccct gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc
    54301 atcgcttcat aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg
    54361 gtacgggccg attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac
    54421 acaatgcggc gctgaacagg tttcagacca tcaccaataa acggcaacgc acggtccatg
    54481 atcacgtaca tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc
    54541 tctgccatat cgctcattaa ttctgattcc tcaacttatt cgccagcctt caaaggggca
    54601 atattgccgg agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag
    54661 cgccgccgga cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaactctt
    54721 tgctgtagac ttgatcggca tagagggcag cacaaggttg cgcccatgcc acaccactcg
    54781 gcatgggggt ccgctccggg cgattcgaga agttgaggtt atgcgagtcc agggaagcct
    54841 aaagcatcaa agtagtcgac tgtaagtata cggttcagcc cagtttagct gtgtctccac
    54901 cagtgccccg cgcctcctgc cataagtgcg gcttgctcat ggatatcccc gagacggctt
    54961 acggcgcacc ggataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
    55021 gcccaacaac aatacgcctg tgacattgtt cgctcaaact tatcgcgagt gatttggctc
    55081 acactccggt aaagattccc catcacgcca acggcacata aacatccgtt tccagtcgct
    55141 gctctgtcac cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt
    55201 tctcgccgct tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt
    55261 ccagctcgcc aacgtggcgg gccacggcat aacgtccacc ggtaagttca ccattgctca
    55321 caccataacg attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg
    55381 cttccggtgc ggtggtggca ggggcgtccc aggcgacgcc aaaagtctgg ctttggttaa
    55441 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacactg tagttgagca
    55501 actcagggct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt
    55561 ttacatccag tgtcaggttt gtcattttgc ctccctgcga aattattccc gggaacgcgt
    55621 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
    55681 gaattccagt ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc
    55741 acaccgttcc agcgtttgtg atcgatatca acattgatag taccgctggc atctttaaac
    55801 acgtagagat cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg
    55861 tcacgcaggg attttgcgct ttctacagtc gttacgctgc cgttcggccc ctgaaatcct
    55921 ccggcctgac tttgcgttac cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc
    55981 ggtgcactgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
    56041 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
    56101 aaaaataaaa tttaatgctg tacagagcgc gttacaacac ggtttactgg cagcaaatac
    56161 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggt
    56221 gacggcatca aaactggcct tagtaaaatg ggttttagtg tcgactggtt tacacaaggt
    56281 cgtcagggaa aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc
    56341 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagagaaagg tcagcgtgag
    56401 ccggtactga acctgaccgc gcgtgatgcg ttagcggaac gtgtagaagg gctgcgtctg
    56461 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggag
    56521 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg
    56581 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa
    56641 gaatttgccc tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg
    56701 attgaagaga aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat
    56761 gtgcatcatc tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc
    56821 tacacattag gtgagaaatg aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc
    56881 taatcttttt aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac
    56941 aaacaacgga taacgtcgat gaattgttcg acacccaact gatgctgttt gccaaacggt
    57001 taagtacgct cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata
    57061 aattaaaaca cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg
    57121 gcagaatggt ccttaatgat ggcgataacg gagaagatat tccctatagc tatcaacggg
    57181 aaggttttgc tgacgggcaa ctggtcggtg aagacgatcc ttggcgtttt gtctggatga
    57241 cctcacctga tggcaaatat cgcatcgttg ttggccagga atgggaatac cgtgaagaca
    57301 tggcgctggc gattgttgcc gggcaattga tcccgtggct ggtcgcactg ccgattatgt
    57361 taatcatcat gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg
    57421 cactacgtat gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg
    57481 aagtgcggcc actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg
    57541 ttcgagaacg acgctttacc tccgatgcag ctcacgaact acgtagcccg ttaacagcgc
    57601 tgaaagtgca aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag
    57661 cactgctcca attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca
    57721 cgctatcgcg gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag
    57781 atctcctaca atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg
    57841 tgcgactgac actcaatgcc cacggcatca aacgcaccgg acaaccgcta ttgctaagtt
    57901 tgttggtgcg aaatttgctg gataacggcc ccggtgtgac accagaggca ctggcgcgaa
    57961 ttggcgaacg cttctatcgc ccacccggac aaaccgctac cggcagtgga cttgggctat
    58021 cgattgtcca gcgaatcgcc aagctgcatg acatgaatgt tgagtttggg aatgcggaac
    58081 aaggtggatt tgaggcgaag gtaagctggt aacttctctg tctatgagag ccgttaaaac
    58141 gactctcata gattttacta atagcaaaat ataaaccgtc cccaaaaaag ccaccaacca
    58201 caaaccagac aaataacgag cgagaataca tatagccaaa actcgaacat ttcccgcata
    58261 aagagttttc ttaagataag aataataagt ggcgtaagaa gaaaaaatgc tgcaatcatt
    58321 gcttgcaata tacgatcttc gcctgaaacg gaaggtatac tgcaaatatt gcctcccgac
    58381 tcaagcatcc agtcatactt attgaaagca aaaaaagaat ctattaacca ataagtaagc
    58441 attatatatg gcaatgcttc aaatattgtt ttaattttct gctttaacat atatcagtac
    58501 gctcataaaa atccatttat aattatcaac atgacacgtt gatattttca tcttttactt
    58561 attcaaagcc atattttcaa tccccaaaaa aaccgaacca aacgtgtaaa ttattccggt
    58621 gcgagtaaga attatgagga atggctatca gtattgtcat tttcagaaaa tatttatcct
    58681 gcatcggtga gtcagagcta gatcagactt ttgctaaatt cgcaaaagac tttgcacatt
    58741 ttgctaattt cacagtaccg ctctgtgacg tactatagtc gacaaacgtc tcaccttgag
    58801 attaaaaaat gagcaacatc ctgattatca acggcgcgaa aaaattcgcc cactccaatg
    58861 gtcaactgaa cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg
    58921 atgtccgcat cgttcgcgcc gacagcgact acaatgtcaa agcggaagta caaaactttc
    58981 tctgggctga tgtggtgatc tggcagatgc caggctggtg gatgggcgcg ccgtggacag
    59041 tgaaaaaata tattgatgat gtattcaccg aaggtcacgg gactctgtat gccagcgatg
    59101 gtcgtacccg caaagatccg tcgaaaaaat acggttccgg cagcctggta caggacaaaa
    59161 aatatatgct ttctctaacc tggaacgcac caatggaagc cttcaccgaa aaagatcagt
    59221 tcttccacgg cgttggcgtt gacggtgtgt atctgccgtt ccataaagca aaccaattcc
    59281 tcggtatgga accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc
    59341 cccgctatac tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctca
    59401 ggctttagaa ggagttaacc atgcttaccg taatcgcaga aatccgtact cgtcctggtc
    59461 aacatcaccg tcaggcggta ttggatcagt ttgctaaaat cgttccaacc gtactgaaag
    59521 aagaaggttg ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt
    59581 ctatggcacc ggattctatc gtgatgattg agcagtggga aagcatcgcg caccttgaag
    59641 cgcatctgca aaccccgcac atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg
    59701 agatgaatat ccgtattctg cagccaggga tttaatcctg ccttgtttgc ccggccatcc
    59761 tgaccgggca atgttctttc ctttaaacct caatctccgc catgtcgcct ttctcttgca
    59821 accagttgcg gcgatcttcc gagcgtttct tcgccagcag catatccatc atcgcgtcag
    59881 tacgctgatc gtcttcatca tcgatagtca actgcaccag acggcgagtg ttcggatcaa
    59941 gcgtggtttc acgcaattgc atcgggttca tttcccccag acctttaaaa cgctggacgt
    60001 tcggcttgcc tttcttgcgt tttaattgct caagtacgcc ctctttctct tcttccgtca
    60061 gcgcgtaata aacctctttc ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa
    60121 cgtgaccgtg tttcaccaac gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg
    60181 caatgtgcag accatcagag tccgcatccg cgaggataca gattttgcca taacgaagct
    60241 ggctcagatc gtcgctgtca ggatcgatac cgatcgctac cgaaatatca tgcacttcct
    60301 gcgaggccag cacttcgtcg gaagaaactt cccaggtgtt aaggatctta cctttcagcg
    60361 gcatgatcgc ctgatattca cgatcgcgcg cctgcttggc agatccgcct gcggagtcac
    60421 cttccacgag gaacagttcg gtacggttaa ggccctgcgc ggtacaatca gccagtttgc
    60481 caggcagcgc cgggccgctg gtcagctttt tacgcaccac ttttttagcc gcacgcatac
    60541 gacgctgggc gctggaaatc gccatctctg ccagcaactc cgccgcctga acgttctggt
    60601 tcagccacag gatgaaggca tctttcacca cgcccgaaac gaatgccgca cactgacgcg
    60661 aagagagacg ctctttggtc tgcccggcaa actgcggatc ctgcatttta accgacagaa
    60721 cataggcgca gcgatcccag atatcttccg ccgacagctt cacgccgcgc ggcagaatgt
    60781 tgcggtattc acagaactca cgcatcgcgt ccaacaggcc ctgacgcaga ccattaacat
    60841 gggtaccgcc ctgcatcgtt gggataaggt tgacgtagct ttcggtcagc agttcaccgc
    60901 cttccggcag ccacagtagc gcccagtcca cagcttcagt atcgccagcg aaattaccga
    60961 taaacggttt ttctggcagc gtcggtaaac cgtttaccgc ttccgccagg taatcattca
    61021 gaccgtcctg atagcaccag cgctgttcgg tgttgttgat ctcatcttta aaggtaatct
    61081 caacgccggg gcacagtact gctttggcct tcagcacatg cgtcaggcgt gaaacagaaa
    61141 aacgcgggct gtcaaagaag gtttcatccg gccagaagtg cacgctggta ccggtattgc
    61201 gtttaccgca agtgccgaca acctgtaaat cctgcacctt ttcgccattt tcaaaggcga
    61261 tgttataaac ctgaccatcg cggcgcacgt taacttctac gcgcttcgac aaggcgttaa
    61321 ctaccgaaat ccccacgcca tgcaggccgc cagagaactg gtaatttttg ttagagaatt
    61381 taccgcctgc atgcagacgg caaagaatca gttcaaccgc cggtactcct tcttccgggt
    61441 gaatatccac cggcatcccg cgcccatcgt caatcacttc taacgactgg tcagcatgta
    61501 agattacatc cacgcgtttt gcgtgacccg ccagtgcttc atcgacactg ttatcaataa
    61561 cttcttgccc caaatggtta gggcgagtgg tatcggtata catccccgga cggcggcgaa
    61621 ccggctcaag cccggtgagt acctcaatgg catcagcgtt ataagtttgc gtcatggttt
    61681 aagttagcaa ttcgagttga tcgtcagaga tggtgcagac caagaaaatc gacgatcggg
    61741 ttgaaataat cttcgaagcc cgtgaatgcg tggttgccgc cttctatgac agtctggcgg
    61801 caggaagcat agtacgccac cgcctggcgg taatccagca cttcatctcc cgtctgttgc
    61861 agcagccaga tcaaatccgg cgcttccagc gggtcaatct gcatgacttt gagatcgtaa
    61921 atatggcgtg actctagcac atattgctgc ccggtgtagg ggttctcgtt ctgaccgaga
    61981 tagtccgtca gcaattcaaa cgggcgtacc gccgggttta ccaccactgc gggcagcata
    62041 aaacattgcg acaaccaggt ggcgtaatat ccccccagtg acgaaccgac aatacccagc
    62101 gaatcgccgc catgttccag gacaatggat tccagcagtt ctgccgcgtc ggaaggatac
    62161 ggcggcaact gcggaatgat catctcaacg tcagggtgat gttccgccag ccagtttttt
    62221 aacaagctcg cttttgcaga gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta
    62281 gacatcagta gccttctgaa gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca
    62341 cctcggtggt cagcgtgcca tcagcatgta actcgagagt acgccagccg ggcgcgatag
    62401 tatccagcgt aaagttggaa cagtgcggct taaactgcac acaggtcgac ggcgttgcca
    62461 gcaggcggcg accgttccag tcgagatcca gctcctgatg aatatgaccg cacagcaagt
    62521 atttgacgtg cggaaacttc gccagcacgg tatccagttc gcccgcgtta cgcagactgt
    62581 gttgatcgag ccaactacaa cccgcaggta gcggatgatg atgcagcagc agcaacgtat
    62641 ggcgttctgg cgcatcggcc agtttacgtt ccagccactc aagctgaaac tcgctcagct
    62701 caccgtgcgg cacgccaaac acctggctat ccagcaacag gatttgccat tgctcaccaa
    62761 taaacacccg cttcgccggg gagatacccg catcctgtaa cgcgctgtac atcgcgggct
    62821 ggaaatcgtg gttgcccggc agccagacgc agggcgcacg aaaacttgcg atgccttcag
    62881 cgaaatgctg ataggccgca gaggattgat cctgcgctaa atcacctgtc gcgacaatca
    62941 ggtcgaattc gtgctggtgt ggacgaatcg cctccagcac cgcctggtag ctttcccagg
    63001 tgtttacccc taacagggct tcgtgctttt gtgcaaacag gtgagtatcg gtaatttgta
    63061 aaatccttcc tctggcctca ccagccagag gaagggttaa caggctttcc aaatggtgtc
    63121 cttaggtttc acgacgctag taaaccggaa tcgccatcgc tccatgtgct aaacagtatc
    63181 gcaaccagtc cgctaaaaac tggttaattt gatgcttttc gtcgcgttga tgcaactttt
    63241 tattaggata atcataccgc gctttgaagc gaaaaatctg ctggcttgaa cacacttcag
    63301 ccaccatcgc gtcatgatac agacgcaccg tcattgacgg aaggctccag taactgatcg
    63361 cgggcgcagt ctgttctatt gtcaccaggg tagtgtatcg ggtcgattcc acaatcgtca
    63421 gccgatattg tgcgtttgcc acctgatagc ttacagtttc gccgggtgcg tcattgcgcg
    63481 gtaacaaacg gcgcaattgt gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt
    63541 caggtgtgta acgcttcatt tatgcccact cattttttaa cgcttgatga tgcagctgca
    63601 gccattgcaa agcgatgacc gacgctgcgt tgtcgatttt cccctcttct acccactggt
    63661 atgcctgttc ccggcttacc acatgaacgc gaatatcttc gttttcatca gccagaccat
    63721 gaataccgct tgcggtcgtg gcgtccactt cgcccaccat aattgacgaa cgctcactgg
    63781 tgccccccgg gcttgccagg aaacttaaca ccggtttggt ccgtttgact atcagtcccg
    63841 cctcttcaat cgcttcgcga cgggcaacat cttccacact ttcaccctct tcaatcatcc
    63901 cggcaaccat ctccagtagc cagggggttt cgctggtgtc gtacgcggca atccgaatct
    63961 gctcaatcag cacaacttca tcacgcactg ggtcaaaggg tagcaagact gcggcgtgac
    64021 cgcgctcaaa aatttcccgc cgtacctcat gactcatttg cccgttgaat agacgatgac
    64081 gaaatctata aagatctaat gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt
    64141 ctacatcgtt tttgccaaat gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc
    64201 tagcactaat agtgaattaa atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc
    64261 atatgttaaa ttgaggcaca ttaacgccct atggcacgta acgccaacct tttgcggtag
    64321 cggcttctgc tagaatccgc aataatttta cagtttgatc gcgctaaata ctgcttcacc
    64381 acaaggaatg caaatgaaga aattgctccc cattcttatc ggcctgagcc tttctgggtt
    64441 cagttcgttg agccaggccg agaacctgat gcaagtttat cagcaagcac gccttagtaa
    64501 cccggaattg cgtaagtctg ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc
    64561 gcgcagtcca ttactgccac agctaggttt aggtgcagat tacacctata gcaacggcta
    64621 ccgcgacgcg aacggcatca actcgaacgc gaccagtgcg tccctgcagt taactcaatc
    64681 catttttgat atgtcgaaat ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca
    64741 ggacgtcaca tatcagaccg atcagcaaac cttgatcctc aacaccgcga ccgcttattt
    64801 caacgtgttg aatgctattg acgttctttc ctatacacag gcacaaaaag aagcgatcta
    64861 ccgtcaatta gatcaaacca cccaacgttt taacgtgggc ctggtagcga tcaccgacgt
    64921 gcagaacgcc cgcgcgcagt acgataccgt gctggcgaac gaagtgaccg cacgtaataa
    64981 ccttgataac gcggtagagc agctgcgcca gatcaccggt aactactatc cggaactggc
    65041 ggcgctgaat gtcgaaaact ttaaaaccga caaaccacag ccggttaacg cgctgctgaa
    65101 agaagccgaa aaacgcaacc tgtcgctgtt acaggcacgc ttgagccagg acctggagcg
    65161 cgagcaaatt cgccaggcgc aggatggtca cttaccgact ctggatttaa cggcttctac
    65221 cgggatttct gacacctctt atagcggttc gaaaacccgt ggtgccgctg gtacccagta
    65281 tgacgatagc aatatgggcc agaacaaagt tggcctgagc ttctcgctgc cgatttatca
    65341 gggcggaatg gttaactcgc aggtgaaaca ggcacagtac aactttgttg gtgccagtga
    65401 gcaactggaa agcgcacatc gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat
    65461 taatgcttct atcagtagta ttaacgccta caaacaagcc gtagtttccg cgcaaagctc
    65521 attagacgcg atggaagcgg gctactcggt cggtacgcgt accattgttg atgtgttgga
    65581 tgcgaccacc acgctgtaca acgccaagca agagctggcg aatgcgcgtt ataactacct
    65641 gattaatcag ctgaatatta agtcagccct gggtacgttg aacgagcagg atttgctggc
    65701 actgaacaat gcgctgagca aaccggtttc cactaatccg gaaaacgttg ccccgcaaac
    65761 gccggaacag aatgctattg ctgatggtta tgcgcctgat agcccggcac ccgtcgttca
    65821 gcaaacatcc gcacgcacta ccaccagtaa cggtcataac cctttccgta actgatgacg
    65881 acgacggggc ttcggccccg tctgaacgta aggcaacgta aagatacggg ttatctgccg
    65941 cattcttccc ccttctcgct tcaatttcga ccagccatcc tctattctga tgggtattta
    66001 ccactggtcc cggaagacaa aaatgaaacg gacaaaatcc atacgccacg catcgttccg
    66061 caaaaactgg agcgcacgcc atctgacacc agtcgctctc gcggttgcca ctgtttttat
    66121 gctggctggc tgtgaaaaga gtgatgaaac agtgtctctc tatcaaaatg ctgacgactg
    66181 ttcagctgca aacccaggca aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa
    66241 agaagccgaa cgtactgcgc cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg
    66301 tgaaggtcag tgtcagcaag caccagccca ggctggcatg gcaccagaaa accaggcgca
    66361 ggctcagcaa tccagcggga gtttctggat gccgctgatg gccggttaca tgatggggcg
    66421 tctgatgggc ggcggcgcgg gatttgcaca gcagccgctg ttctcctcga aaaacccggc
    66481 cagtccggct tacggtaaat ataccgacgc gacgggtaaa aactatggcg cagcccagcc
    66541 aggccgcacc atgaccgtac cgaagacggc aatggcacca aaaccggcga ccaccactac
    66601 cgttacccgt ggcggttttg gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc
    66661 aaccggcacc tcttctcgtt caatgggtgg ctgataccga tggaaagagt cagtattacc
    66721 gagcgcccgg actggcgtga gaaagcccac gaatacggtt tcaattttca caccatgtac
    66781 ggcgagccgt actggtgtga agatgcttac tacaagttga ccctcgccca ggttgaaaag
    66841 ctggaagaag tcaccgccga actgcaccag atgtgcctga aagtggtgga aaaagtgatc
    66901 gccagcgatg agctgatgac caaattccgc ataccaaaac acacctggag ttttgtgcgt
    66961 caatcatggc tgactcacca gccatcgctt tattcgcgtc ttgatctggc gtgggatggc
    67021 actggcgaac ctaaacttct ggaaaataac gccgatacgc caacgtcact atacgaggcg
    67081 gcgttctttc agtggatctg gctggaagat cagcttaacg ccggtaactt gccggagggc
    67141 agcgaccagt ttaacagtct gcaagaaaaa ctgatcgatc gcttcgttga gctgcgtgaa
    67201 cagtatggct tccagttgct gcatctcacc tgctgtcgcg acacggtgga agatcgcgga
    67261 accattcagt atttgcagga ctgcgcaacc gaagctgaaa tcgccaccga gttcctctac
    67321 atcgacgaga taggtttagg cgaaaaaggc cagttcacgg atttacagga tcaggtgatt
    67381 tctaacctgt tcaagcttta tccgtgggaa tttatgctgc gcgaaatgtt ctccaccaag
    67441 ctggaagatg ctggcgtacg ctggctggaa ccggcgtgga agagcattat ctccaacaag
    67501 gcacttctac cgctactgtg ggagatgttc ccgaatcacc cgaacctgct gcccgcttat
    67561 tttgcggaag atgatcatcc gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt
    67621 gaaggcgcaa acgtgtcgat cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg
    67681 tatggcgaag aagggatgat tgttcagcaa ttccacccgt taccgaaatt cggcgacagc
    67741 tatatgctga ttggtagctg gctggtgaac gatcaacctg ccggaattgg cattcgtgaa
    67801 gaccgtgcgt tgatcaccca ggatatgtct cggttttatc cacatatttt tgttgaataa
    67861 gccacgatac cggatggcac tcgccatccg gtaattgtta gcctatctgc accgacagca
    67921 tacttaaact gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc
    67981 acacacctaa cacatacaac aacggcagat agtgctctgg cgttgggttc gataacgcgc
    68041 caccttcatg gtcgagataa ttcaccagag gatgctgctc taccggccct tgccacgtca
    68101 gattcgcttt cacatactca ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt
    68161 gccacttcac tgtgcgcagg ttatgcacca cgttaccgct ggcgaccaac attattcctt
    68221 catcacgcag cgccgcgagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac
    68281 tgtcgatact caactgcacc atagggatat cggcgtcagg atacatctta atcagcacgc
    68341 cccacgagcc gtggtcaaag ccccaggctt ctttatccag cgtcaccggg atcggcgcta
    68401 acagctcaac cagacgctgt gccagcgcag gcgaacccgg agcaggataa tgcgtatcgt
    68461 acagcgcctg cgggaagcca ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca
    68521 ctcctgttcc acgggtaaac cagtgagccg aaaccaccac aatcgcttgc gggcgtggca
    68581 atgtcatccc caacttctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg
    68641 gactaccgtg acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa
    68701 ctaaaggtgt cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat
    68761 aacaatgatg atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg
    68821 agttgtcgat gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg
    68881 gcgcgtttct cggcgttctc ggacaaaaac cctcgaaccg cttactggcg ttttcgctag
    68941 gttttgcggc ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcactag
    69001 cagctgaagg aatgtcgccg gtgttgggtt atggaatgtt tatcttcggt ctgcttggct
    69061 actttggcct ggaccgcatg ttgccgcatg cgcatccgca ggatttaatg caaaaatcgg
    69121 tgcagctgtt gccaaaatcg atcaagcgca cagccattct gctcactctc ggcatcagtc
    69181 tgcataactt cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg
    69241 gatttggcat cgcactggcc gtcgcgttgc acaatatccc tgaaggtctg gcagtggcag
    69301 gcccggttta tgcggcaacg gggtctaaac gcaccgcgat tctgtgggcg gggatttctg
    69361 gactagcaga aattcttggt ggtgtgctgg cgtggttaat cctcggtagc atgatttccc
    69421 cggtggtcat ggcggcaatc atggcggcgg ttgcaggaat tatggtggcg ctctcggttg
    69481 atgaattaat gccgctcgcc aaagagattg accctaataa taaccccagc tatggcgtac
    69541 tatgtggaat gtcagtgatg ggattcagtt tagtgctgct acaaacggcg ggaattggtt
    69601 aaaaacaaca tcagaagttg tccagcacat ggacaacttc tgccagcttc acgaaagcct
    69661 tattctacct tttcggctgt ctcttctctc gtactgttta agtatttgtt ccgcgcttca
    69721 agcagtgcgt ctctgtcctg gcgaaacgcc cgacaataac gtttcaaatg caggaaataa
    69781 cccaccacaa ccagagcaat caatacaatc cagtaccagg cgataaacat ccttttaccc
    69841 ttaatattta acaatactaa acatttagcg cataaatttc acatatcctt tttcggatat
    69901 attcatatgg tcgcataata acggaacaat tatatgattt tgatcataat gaaaaagaat
    69961 gcctgatatt aatgaaattt tctcatagaa agagaagcgg aattaacatc ttgcatagca
    70021 gggcaaaata aaaaccgaag ccagctacaa cttcggtttc ttgattaagg cagtaattaa
    70081 gcagcggttt tcagctggct ttacgctcat gtgcctgacg gtatgccacc aggtcttcaa
    70141 tagtcacgag cgccatattg tgtttattgg caaactcaat gcactctggt gcacgcgcca
    70201 tcgtgccatc gtcattagtc agctcacaca gcacaccagc cggtttaaag cctgccagcg
    70261 tcatcaaatc aatagtcgct tcagtatgac cgccacgcgt cagtacacca cctgcctgcg
    70321 cgcgaagtgg gaaaacgtgg cccgggcgat tcagatcaga aggttttgcg ccatcggcaa
    70381 tcgctgcgcg aacggtcgta atacgatcag cggcagaaac accggtagtc acaccttcag
    70441 ccgcttcaat ggtcacggta aaaccggtgc cataggcgct ggtgttattt tctaccatca
    70501 ttggcagatc aagttgttta cggcgatctt cagtgatgca caggcaaaca ataccgctac
    70561 cgtggcgaat ggtcagcgcc atctgctcaa cggtcatggt ttctgccggg aagatcatat
    70621 cgccttcgtt ttcacggtct tcatcatcaa gcaccattac accgcgtcct tcacgcagcg
    70681 cagccagtgc attttcaaca cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat
    70741 tcatggtaaa aaaacctcac taaaattatg gttaccagaa tcagggcagt cttaggagtg
    70801 gcggcatata gccaaaataa cgtgagcggg tccatgcccg acagaatcgt tactctctcc
    70861 catccggact ctaaccgtcg gccccggaat tacaccggat ctgctgtcct ttgagttcgc
    70921 acccaaagcg ctcgcgggct ttcaactgag ttgatttacc gccggtgggg aatttcgccc
    70981 cgccctaaga ataagcggat tcactataac gctaatgatt agcggcagca acgcatagct
    71041 tcacataatt ctggtttatg acttaccctt atcgcactac aatggtactc aacacctatc
    71101 agtacaggga aactacaatg attgacccga aaaaaattga gcaaatcgct cgccaggttc
    71161 acgaatcaat gcctaaagga atcagggagt tcggggaaga tgtggagaaa aaaatccgcc
    71221 aaaccctaca agctcagctg acgcgcctcg accttgtaag ccgtgaagag ttcgacgtcc
    71281 aaacgcaagt cctgttacgg actcgtgaga aactggcgct gcttgaacaa agaatcagcg
    71341 aactggaaaa tcgcag
//
LOCUS       DAJTYL010000004        70226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYL010000004 DAJTYL010000000
VERSION     DAJTYL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.004"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..507
                     /locus_tag="OO572_000249"
     CDS             <1..507
                     /locus_tag="OO572_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6659380.1"
                     /translation="YLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQEN
                     RLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGA
                     LASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSV
                     LQGTAQCH"
     gene            518..1609
                     /locus_tag="OO572_000250"
     CDS             518..1609
                     /locus_tag="OO572_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6659381.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1652..2512)
                     /gene="rsmI"
                     /locus_tag="OO572_000251"
     CDS             complement(1652..2512)
                     /gene="rsmI"
                     /locus_tag="OO572_000251"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6659382.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2577..4613
                     /gene="lpoA"
                     /locus_tag="OO572_000252"
     CDS             2577..4613
                     /gene="lpoA"
                     /locus_tag="OO572_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6659383.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4571..4966
                     /locus_tag="OO572_000253"
     CDS             4571..4966
                     /locus_tag="OO572_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6659384.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4986..5576
                     /gene="diaA"
                     /locus_tag="OO572_000254"
     CDS             4986..5576
                     /gene="diaA"
                     /locus_tag="OO572_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6659385.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5586..6161
                     /gene="yraP"
                     /locus_tag="OO572_000255"
     CDS             5586..6161
                     /gene="yraP"
                     /locus_tag="OO572_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6659386.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6275..7315)
                     /locus_tag="OO572_000256"
     CDS             complement(6275..7315)
                     /locus_tag="OO572_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6659387.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7388..8023)
                     /locus_tag="OO572_000257"
     CDS             complement(7388..8023)
                     /locus_tag="OO572_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6659388.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8151..8669
                     /gene="yhbO"
                     /locus_tag="OO572_000258"
     CDS             8151..8669
                     /gene="yhbO"
                     /locus_tag="OO572_000258"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6659389.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8649..9092)
                     /locus_tag="OO572_000259"
     CDS             complement(8649..9092)
                     /locus_tag="OO572_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6659390.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9143..9445
                     /gene="yhbQ"
                     /locus_tag="OO572_000260"
     CDS             9143..9445
                     /gene="yhbQ"
                     /locus_tag="OO572_000260"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6659391.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9432..9935)
                     /locus_tag="OO572_000261"
     CDS             complement(9432..9935)
                     /locus_tag="OO572_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6659392.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9929..10453)
                     /locus_tag="OO572_000262"
     CDS             complement(9929..10453)
                     /locus_tag="OO572_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6659393.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10662..11657
                     /locus_tag="OO572_000263"
     CDS             10662..11657
                     /locus_tag="OO572_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6659394.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11666..12544
                     /locus_tag="OO572_000264"
     CDS             11666..12544
                     /locus_tag="OO572_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6659395.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12625..13632
                     /locus_tag="OO572_000265"
     CDS             12625..13632
                     /locus_tag="OO572_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6659396.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13750..14994)
                     /gene="mtr"
                     /locus_tag="OO572_000266"
     CDS             complement(13750..14994)
                     /gene="mtr"
                     /locus_tag="OO572_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6659397.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15148..17037)
                     /gene="deaD"
                     /locus_tag="OO572_000267"
     CDS             complement(15148..17037)
                     /gene="deaD"
                     /locus_tag="OO572_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6659398.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17030..17110)
                     /gene="yrbN"
                     /locus_tag="OO572_000268"
     CDS             complement(17030..17110)
                     /gene="yrbN"
                     /locus_tag="OO572_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6659399.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17217..18101)
                     /gene="nlpI"
                     /locus_tag="OO572_000269"
     CDS             complement(17217..18101)
                     /gene="nlpI"
                     /locus_tag="OO572_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6659400.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18210..20345)
                     /gene="pnp"
                     /locus_tag="OO572_000270"
     CDS             complement(18210..20345)
                     /gene="pnp"
                     /locus_tag="OO572_000270"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6659401.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20592..20861)
                     /gene="rpsO"
                     /locus_tag="OO572_000271"
     CDS             complement(20592..20861)
                     /gene="rpsO"
                     /locus_tag="OO572_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6659402.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21010..21954)
                     /gene="truB"
                     /locus_tag="OO572_000272"
     CDS             complement(21010..21954)
                     /gene="truB"
                     /locus_tag="OO572_000272"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6659403.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21954..22355)
                     /gene="rbfA"
                     /locus_tag="OO572_000273"
     CDS             complement(21954..22355)
                     /gene="rbfA"
                     /locus_tag="OO572_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6659404.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22519..25167)
                     /gene="infB"
                     /locus_tag="OO572_000274"
     CDS             complement(22519..25167)
                     /gene="infB"
                     /locus_tag="OO572_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6659405.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25192..26679)
                     /gene="nusA"
                     /locus_tag="OO572_000275"
     CDS             complement(25192..26679)
                     /gene="nusA"
                     /locus_tag="OO572_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6659406.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26707..27159)
                     /gene="rimP"
                     /locus_tag="OO572_000276"
     CDS             complement(26707..27159)
                     /gene="rimP"
                     /locus_tag="OO572_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6659407.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27366..27442)
                     /locus_tag="OO572_000277"
     tRNA            complement(27366..27442)
                     /locus_tag="OO572_000277"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27406..27408),aa:Met,seq:cat)
     gene            27790..29133
                     /gene="argG"
                     /locus_tag="OO572_000278"
     CDS             27790..29133
                     /gene="argG"
                     /locus_tag="OO572_000278"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6659408.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29141..30748)
                     /locus_tag="OO572_000279"
                     /pseudo
     CDS             complement(29141..30748)
                     /locus_tag="OO572_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31226..31312)
                     /locus_tag="OO572_000280"
     tRNA            complement(31226..31312)
                     /locus_tag="OO572_000280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31276..31278),aa:Leu,seq:gag)
     gene            complement(31327..31659)
                     /gene="secG"
                     /locus_tag="OO572_000281"
     CDS             complement(31327..31659)
                     /gene="secG"
                     /locus_tag="OO572_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6659409.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31887..33224)
                     /gene="glmM"
                     /locus_tag="OO572_000282"
     CDS             complement(31887..33224)
                     /gene="glmM"
                     /locus_tag="OO572_000282"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6659410.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33217..34065)
                     /gene="folP"
                     /locus_tag="OO572_000283"
     CDS             complement(33217..34065)
                     /gene="folP"
                     /locus_tag="OO572_000283"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6659411.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34155..36089)
                     /gene="ftsH"
                     /locus_tag="OO572_000284"
     CDS             complement(34155..36089)
                     /gene="ftsH"
                     /locus_tag="OO572_000284"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6659412.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36189..36818)
                     /gene="rlmE"
                     /locus_tag="OO572_000285"
     CDS             complement(36189..36818)
                     /gene="rlmE"
                     /locus_tag="OO572_000285"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6659413.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36944..37237
                     /gene="yhbY"
                     /locus_tag="OO572_000286"
     CDS             36944..37237
                     /gene="yhbY"
                     /locus_tag="OO572_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6659414.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37393..37869)
                     /gene="greA"
                     /locus_tag="OO572_000287"
     CDS             complement(37393..37869)
                     /gene="greA"
                     /locus_tag="OO572_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6659415.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38117..39550
                     /gene="dacB"
                     /locus_tag="OO572_000288"
     CDS             38117..39550
                     /gene="dacB"
                     /locus_tag="OO572_000288"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6659416.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39736..40908)
                     /gene="cgtA"
                     /locus_tag="OO572_000289"
     CDS             complement(39736..40908)
                     /gene="cgtA"
                     /locus_tag="OO572_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6659417.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40924..41889)
                     /locus_tag="OO572_000290"
     CDS             complement(40924..41889)
                     /locus_tag="OO572_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6659418.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42016..42273)
                     /gene="rpmA"
                     /locus_tag="OO572_000291"
     CDS             complement(42016..42273)
                     /gene="rpmA"
                     /locus_tag="OO572_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6659419.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42294..42605)
                     /gene="rplU"
                     /locus_tag="OO572_000292"
     CDS             complement(42294..42605)
                     /gene="rplU"
                     /locus_tag="OO572_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6659420.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42863..43834
                     /gene="ispB"
                     /locus_tag="OO572_000293"
     CDS             42863..43834
                     /gene="ispB"
                     /locus_tag="OO572_000293"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6659421.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44061..44339
                     /gene="sfsB"
                     /locus_tag="OO572_000294"
     CDS             44061..44339
                     /gene="sfsB"
                     /locus_tag="OO572_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6659422.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44387..45646)
                     /gene="murA"
                     /locus_tag="OO572_000295"
     CDS             complement(44387..45646)
                     /gene="murA"
                     /locus_tag="OO572_000295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6659423.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45701..45955)
                     /gene="ibaG"
                     /locus_tag="OO572_000296"
     CDS             complement(45701..45955)
                     /gene="ibaG"
                     /locus_tag="OO572_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6659424.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46115..46408)
                     /gene="mlaB"
                     /locus_tag="OO572_000297"
     CDS             complement(46115..46408)
                     /gene="mlaB"
                     /locus_tag="OO572_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6659425.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46408..47043)
                     /gene="mlaC"
                     /locus_tag="OO572_000298"
     CDS             complement(46408..47043)
                     /gene="mlaC"
                     /locus_tag="OO572_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6659426.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47062..47613)
                     /gene="mlaD"
                     /locus_tag="OO572_000299"
     CDS             complement(47062..47613)
                     /gene="mlaD"
                     /locus_tag="OO572_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6659427.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47618..48400)
                     /gene="mlaE"
                     /locus_tag="OO572_000300"
     CDS             complement(47618..48400)
                     /gene="mlaE"
                     /locus_tag="OO572_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6659428.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48408..49217)
                     /gene="mlaF"
                     /locus_tag="OO572_000301"
     CDS             complement(48408..49217)
                     /gene="mlaF"
                     /locus_tag="OO572_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6659429.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49427..50392
                     /locus_tag="OO572_000302"
     CDS             49427..50392
                     /locus_tag="OO572_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6659430.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50406..51392
                     /gene="kdsD"
                     /locus_tag="OO572_000303"
     CDS             50406..51392
                     /gene="kdsD"
                     /locus_tag="OO572_000303"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6659431.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51413..51979
                     /gene="kdsC"
                     /locus_tag="OO572_000304"
     CDS             51413..51979
                     /gene="kdsC"
                     /locus_tag="OO572_000304"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6659432.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51976..52551
                     /gene="lptC"
                     /locus_tag="OO572_000305"
     CDS             51976..52551
                     /gene="lptC"
                     /locus_tag="OO572_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6659433.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52520..53077
                     /gene="lptA"
                     /locus_tag="OO572_000306"
     CDS             52520..53077
                     /gene="lptA"
                     /locus_tag="OO572_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6659434.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53084..53809
                     /gene="lptB"
                     /locus_tag="OO572_000307"
     CDS             53084..53809
                     /gene="lptB"
                     /locus_tag="OO572_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6659435.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53857..55290
                     /gene="rpoN"
                     /locus_tag="OO572_000308"
     CDS             53857..55290
                     /gene="rpoN"
                     /locus_tag="OO572_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6659436.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55313..55600
                     /gene="hpf"
                     /locus_tag="OO572_000309"
     CDS             55313..55600
                     /gene="hpf"
                     /locus_tag="OO572_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6659437.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55718..56209
                     /gene="ptsN"
                     /locus_tag="OO572_000310"
     CDS             55718..56209
                     /gene="ptsN"
                     /locus_tag="OO572_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6659438.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56255..57109
                     /gene="rapZ"
                     /locus_tag="OO572_000311"
     CDS             56255..57109
                     /gene="rapZ"
                     /locus_tag="OO572_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6659439.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57106..57378
                     /gene="npr"
                     /locus_tag="OO572_000312"
     CDS             57106..57378
                     /gene="npr"
                     /locus_tag="OO572_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6659440.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57591..58223
                     /gene="yrbL"
                     /locus_tag="OO572_000313"
     CDS             57591..58223
                     /gene="yrbL"
                     /locus_tag="OO572_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6659441.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58220..58947)
                     /gene="mtgA"
                     /locus_tag="OO572_000314"
                     /pseudo
     CDS             complement(58220..58947)
                     /gene="mtgA"
                     /locus_tag="OO572_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(58944..59597)
                     /gene="elbB"
                     /locus_tag="OO572_000315"
     CDS             complement(58944..59597)
                     /gene="elbB"
                     /locus_tag="OO572_000315"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6659442.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59827..62163)
                     /gene="arcB"
                     /locus_tag="OO572_000316"
     CDS             complement(59827..62163)
                     /gene="arcB"
                     /locus_tag="OO572_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6659443.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62259..63188)
                     /locus_tag="OO572_000317"
     CDS             complement(62259..63188)
                     /locus_tag="OO572_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6659444.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63863..68323
                     /gene="gltB"
                     /locus_tag="OO572_000318"
     CDS             63863..68323
                     /gene="gltB"
                     /locus_tag="OO572_000318"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6659445.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68336..69754
                     /gene="gltD"
                     /locus_tag="OO572_000319"
     CDS             68336..69754
                     /gene="gltD"
                     /locus_tag="OO572_000319"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6659446.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69938..>70226
                     /locus_tag="OO572_000320"
     CDS             69938..>70226
                     /locus_tag="OO572_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659447.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLF"
BASE COUNT        17186 a        19056 c        17448 g        16536 t
ORIGIN      
        1 tatctgggca acgcttttgc gcttatcgat gctaacgggg cttctggcgt gaggatacaa
       61 aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact
      121 tatcaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgaa
      181 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
      241 aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt
      301 ggcgctcttg ccagcaacga tgatacgggg caacaaagta tcgtcgatga gggcggcata
      361 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
      421 gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta
      481 ttacaaggca cagcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
      541 aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagccga
      601 cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac
      661 agataacgtg acaggacggg agtttacctc tgcaacgcta agtagcacta actggcaata
      721 cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccccgtact
      781 taccaccact ggacatcaga caggatatta caaactcaat gacagcctgg atattaaaac
      841 cacattacag gcaaacgaca ttccaggact cacaaccgac caggttgtct ctgttaacac
      901 ccgattcaca cagataaaaa gcagcaccgt atattctgct gcaacccaaa cgggtgtttg
      961 ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct
     1021 gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat
     1081 taaaggcgcg tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt
     1141 caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga
     1201 tgttattaaa gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat
     1261 gccagacggt tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa
     1321 gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa
     1381 cctggtcgcc aggcgaagaa gttcagacaa tgtgcccgat gtcggtattc gtattgaaaa
     1441 tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc
     1501 cgggcatggc accgtcaata tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga
     1561 aacaggaaaa tttcagggca cagccaccat taccgtcatg gtgcggtaaa agaagacgtt
     1621 gcgttttgat taacgcaacg cttcggcact gttacccctg ctgctccagc gcatacttat
     1681 acagcgcatt tttcttcacg ccatgaattt ctgcggccag cgccgccgct tttttcagcg
     1741 gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
     1801 gtgctttatg accttcgaca atcagcacca tttcgccttt gcgacggttt tcatcttcct
     1861 ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
     1921 gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
     1981 tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg
     2041 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac
     2101 ggtcagatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggt ccgggtagcg
     2161 gcaccacacg aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa
     2221 ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc agctttgcca
     2281 gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
     2341 tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa
     2401 cggcctgtaa tacctctaac gtacgctggg tgatatccgc cagattgccg attggcgtcg
     2461 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
     2521 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg
     2581 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
     2641 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
     2701 cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca
     2761 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg
     2821 cggttgagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa
     2881 cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
     2941 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa
     3001 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
     3061 aaccgctgct tggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct
     3121 cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
     3181 aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga
     3241 aagccgggat cgccgactgg cagaaacgtt atccgaacaa tccgggcgcg aaaatgctgc
     3301 caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc
     3361 tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
     3421 ccgcgaaaaa tatcggcact cagccagtgg cggctcaggt agctgccgca cctgccgcag
     3481 acgtagcaga acaacctcag ccgcaaactg cggatagcgt tgccagcccg gcacaagcct
     3541 cggttagcga tctgaccggt gatcagcctg cggcccagcc ggtgcctgta agcgccccgg
     3601 cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg
     3661 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta
     3721 ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacaccccgc
     3781 tgaacgtact ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat atttgttact
     3841 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
     3901 aacaagcgcc gctggtgctg atcccacgca gtgcattggg cgatcgcgta gccaatgcgt
     3961 ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca
     4021 ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga
     4081 ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
     4141 cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtc tacattgtgg
     4201 caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga
     4261 gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg caccgcaggc ccggatttcc
     4321 gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctgc
     4381 cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct cgcatgtatg
     4441 cgatgggcgt tgatgcctgg tcgttggcaa atcatttctc acaaatgcgc caggttcagg
     4501 gttttgaaat caacggtaat accggaagtc tgacggctaa cccggattgc gtgattaaca
     4561 ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa
     4621 acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg
     4681 gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
     4741 cctgaccacc gtttttgtcg aggtacgcta tcgccgctct gcgctttatg gcggcgcggc
     4801 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
     4861 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
     4921 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
     4981 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg
     5041 cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc
     5101 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
     5161 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
     5221 taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt
     5281 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
     5341 gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt gatatgacca
     5401 ttgtggcatt gaccggctat gacggcggcg aacttgcagg tttgttaggg ccacaggatg
     5461 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
     5521 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gattaaggag
     5581 aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt
     5641 gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac
     5701 gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
     5761 cgaaagacga acagattaag aaagaaaccc gtattaatgt cacggcctat cagggcaaag
     5821 tgctgctggt tgggcagtca ccaaatgctg aactttcggc tcgcgccaaa cagattgcta
     5881 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
     5941 tgggcgaagc atctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca
     6001 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
     6061 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg
     6121 gcgtaaagcg ggttaccaca gcgtttacgt ttattaaata gcagcccctt gtaatgcctg
     6181 atgcgacgct tgccgcgtct tatcaggcct acaacgatgc aaaccgtagg tcggataagg
     6241 cgtttacgcc gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga
     6301 caatcacacc agacactgcc accatcgccc ctgtcagcca taaggctttc gccgggaacg
     6361 ctttgcgcag cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca
     6421 gcgccggagc ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg
     6481 ccgtgggaat gacaaacaag catcctgcta ccgccatcgc caccacccac atcaggctgt
     6541 tatcgacagc accatcggca tgggggaata accagacgcg tgcggcaccc aacaccagta
     6601 ctgcaaggat gtaaaccggg atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc
     6661 ggctaaaaaa accgctctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg
     6721 gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg
     6781 ccaccagacg aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg
     6841 ccgggtttaa caccggattg cccatccaga atgccagcgc accgcccatc gatacctgtt
     6901 ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg
     6961 aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc
     7021 agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt
     7081 tccatactgc gaggaaatag atcatcgcgt aatccaacgc cgcttgccac ggattagcat
     7141 ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggctgcagta aacgctttgc
     7201 cgtagtaagg ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg
     7261 cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat
     7321 tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg
     7381 ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat
     7441 gctcaggccg catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt
     7501 tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt
     7561 tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg
     7621 caattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat
     7681 tggcacccat agcactgacc accagcatat gctgcgcgcc cagtcgccgc ccggttaatg
     7741 cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc
     7801 gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg
     7861 cgtcactcag ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc
     7921 gcgtcggcgc agcaatggcg ttaactttcg gttcattaat caacatccgc agcaggtgac
     7981 cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgtt cctttacaga
     8041 attgtctgcc ttgcgctccc cggctcatgc accagtctta atagaccatg aggtaattat
     8101 ccccgattgt ggaaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga
     8161 aaattgccgt tttaatcact gatgaatttg aagattcaga atttacttca cccgcagacg
     8221 agttccgtaa agccggacac gaagtgatta ctattgaaaa acaagcgggt aaaacggtga
     8281 aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgccag
     8341 cggagtttga tgccctgttg ctaccgggtg gtcattcacc ggattatctg cgtggcgaca
     8401 accgttttgt cacctttacc cgtgattttg tgaatagcgg caaacctgtt ttcgccatct
     8461 gtcacggtcc gcagctgctg atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag
     8521 ttaaaccgat cattattgat gttaaaaatg cgggcgtgga attttacgat caggaagtcg
     8581 tggttgataa agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc
     8641 gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag
     8701 cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga tttcccacac cggggccgac
     8761 agcattctgg caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc gctttcctca
     8821 ccttccagcc tgcggatctc acctttaaac tgcacaccgc gaattaacgc taccgttttc
     8881 ggctgaccgt ttaccgttcc ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc
     8941 gttttttctt ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca
     9001 ttagcgcacc agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc
     9061 agccaacggc tgatggcgat gagtgtttcc attgctgttc tcttttatac tgtgggcctg
     9121 accttaacat gcgatgcaga cgatgacacc ctggtttctt tacttgatcc gtaccgccga
     9181 caataagctt tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag
     9241 cggcaaaggg gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc
     9301 agtgggcgac cgttcgctgg cactacgggc ggaatatcgc gttaagcaac tgaccaaacg
     9361 gcaaaaagag cgtctggtag cggaaggcgc agggtttgca gaactgttga gcagcctgca
     9421 aaccccggag attaaaagcg attgaaatgc tcgtgatact caaccagacc ggttacgcca
     9481 ttcagcgcgt catccgctaa acgatgtacc aggaaggcgc tttcagtgcc cggccagcgg
     9541 caacgcagat cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg
     9601 cccagcgtca ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc
     9661 agttggcgtg ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg
     9721 acccattgca ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc
     9781 tgaccttcgt catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg
     9841 tgaaccagct tcgcttccgc atcactttcg aatgagcgac gcagcagggc atcaataccc
     9901 ggcgcatcaa tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg
     9961 tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc
    10021 gtaaggcttt cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacaccc
    10081 ccagctccgt atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt
    10141 gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt
    10201 gcgtgttctg gctgaccacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa
    10261 tatcacgcac atgaatactt aaccagcggc cttcaagaaa ctccagctcg ccatcatcca
    10321 gcgcctgacg gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg
    10381 gcgtcagttt taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca
    10441 gtttatccaa cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga
    10501 aaaataacat agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca
    10561 actttaactg ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt
    10621 aacatttttg cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg
    10681 ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg
    10741 ggctaaaaga tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat
    10801 tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca
    10861 cttttgcgca tccggacggt tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc
    10921 tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt
    10981 acccgcatat tgaacgtcat gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta
    11041 acttttatca tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc
    11101 atcaggtgaa acagctggca cgggttactc ctgtcccgct ggaagtgttt gctttcggca
    11161 gcctgtgtat tatgtcggaa ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc
    11221 ccaacactgt gggtgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg
    11281 ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag
    11341 gttatccgac gctgtgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg
    11401 aagaaccaac cagtctcaat accctggaac tgctgccgga gttaatggct gcgaatattg
    11461 cttcggtgaa aattgaaggc cgccagcgta gcccggcgta tgtcagccag gcggcgaaag
    11521 tctggcgtca ggctatcgac cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg
    11581 cgtggatgga gacgctcggg tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc
    11641 accgtaaatg gcagtgagaa gagcaatgaa atattcctta gggccagtgc tgtggtactg
    11701 gccaaaagag acgctggaag atttttatca gcaagccgcc gccagcagcg ccgacgtgat
    11761 ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg actgtctgga
    11821 gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt
    11881 gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt gagaacggtg aatttctgat
    11941 tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc
    12001 cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat
    12061 gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca
    12121 gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct
    12181 accgctggcc tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga
    12241 gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca
    12301 acaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa
    12361 cgagctggca tccatgcagg gattggttga tgtagtccgc ctgtcaccgc agggcactga
    12421 cactttcgcg atgctcgacg ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt
    12481 gacggcaaac agcgactgta acggctactg gcggcggctg gcaggactgg agctgcaagc
    12541 ctaagtaaat agctcacttt gttaacaact ttaactattc tttaatgcag tattaaagat
    12601 taatcggtaa caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat
    12661 ctggccccca tacccgaagg ctcttcagcg cgggaagcgt tctcccactc tctcgatctc
    12721 gcccgtctgg ctgaaaagcg cggctatcat cgctactggc tggcagaaca ccacaatatg
    12781 accggcattg ccagtgctgc aacatcggta ttgattggct atctggcggc gaataccacc
    12841 acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc acttaccgtt ggtcattgcc
    12901 gaacagttcg gcacactcaa tacgctctat ccggggcgaa tcgatttggg gctgggtcgt
    12961 gctccgggta gtgaccaacg gacaatgatg gcgctacgtc gtcatatgag cggcgatatt
    13021 gataatttcc cccgcgatgt cgcggagctg gtggactggt ttgacgcccg cgatcccaat
    13081 ccgcatgtgc gcccggtacc aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc
    13141 agcctttaca gtgcacaact ggcggcgcag cttggtctgc catttgcgtt tgcctcacac
    13201 ttcgcgccgg atatgttgtt ccaggcgctg catctttatc gcagtaactt caaaccgtcg
    13261 gctcggctgg aaaaaccata cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac
    13321 cgcgacgctg aatttctgtt tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc
    13381 gaaaccgggc aactgccgcc gccgattcaa aatatggatc agttctggtc accgtctgag
    13441 cagtatgacg tgcagcaggc gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt
    13501 catggtttgc agtcgatcct gcgcgaaacc gacgctgatg agattatggt taacgggcag
    13561 attttcgacc accaggcgcg cctgcattcg tttgagctgg cgatggatgt taaggaagag
    13621 ttgttgggat agtgtgtctt aacgcgggaa gccttatccg agctggcaac gctgtcctac
    13681 gtaggcctga taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg tcggataagg
    13741 caccgctgat tactgataca ccggcagtaa attaaagctc gataaaatat gcaccagtgc
    13801 gttgccgacg ccaaacacca gaatcagcgc aatcatcggc ttgccacccc agacgcggaa
    13861 tttcgggctg ccaaagcgtt tacgcgatgc gcgggccagc agtgccggaa caattgccgc
    13921 ccagatggta gccgctaaac cagcataacc aatggcatac aggaatccgt tcgggaacag
    13981 cagaccaccc acaacaggcg gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc
    14041 cgagtcgtcg aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccaag
    14101 gaacgaactc gctaccgcaa agtttgagaa cacgaccagc agaaggtcca ggctacggct
    14161 gttcagcacg ccgcttaatg cttgtaccaa cacatcaata ttaccgccct tctcggcgat
    14221 gccgataaac tccggacgag ggatgttgcc cattgtcgcc agcaaccaga tggtatacag
    14281 cgccagtgcc atcagcgtac cgtacaccag gcatttcacg atggttttcg gatctttgcc
    14341 gtaatacttc atcaggcttg gcacgttacc gtgataacca aacgacgcca ggcagaacgg
    14401 cagggtcatc agcaaatacg gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt
    14461 cgcaggctgc acatgcccca gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc
    14521 ccccagcaca atcgccgtca tgcggctgac ggctttagta ctcaaccaca ccacaaacgc
    14581 taccagcagt gcaaaaccaa aacccgccgc ccgtgccggg acgtttagcg acatctctgc
    14641 gaaggtgtga tgcagaatcg aaccactggc agaaatatag gcataggtca ggatatagag
    14701 cacaaaggca atggaaatgc cgttgaccac gttccagcct ttgcccagca aatctttggt
    14761 gatggtgtca aaactcgaac cgattcggta attcaggtta gcttccagaa tcatcaagcc
    14821 ggaatgcagc atacagaacc aggtaaagat cagcgctgcc attgaccaga aaaaccacgc
    14881 cccggacatg accactggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat
    14941 caccacgccg ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc
    15001 ttctctccag tgaaaaatag tgcgactgcg ttgttatgca ttgcactgta ccagtacatg
    15061 agtacaaaag acagaaaaaa agccccgatg gttaaaatcg gggctgtata tattatttta
    15121 cagattgtgt tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt
    15181 agagtcatca cgacgcggtg cgcggccttc acgacgttcg ccgctaaaac gacgaccatc
    15241 accacgacca ccttcacggc gttcaccgct gaagttacga ccgccttcac gacgctcgcc
    15301 accgaaacca cgaccaccgc cacgacgctc accgccagta tgcggctgtg catcgcccag
    15361 taactgcatg ttcatcggct tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc
    15421 acccggcata cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc
    15481 gatgtaacgg ctgctgatgt cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc
    15541 aacaccatca tcgcggccca cttcaatgcg gtacagctgc atatcgccaa catcacgacg
    15601 ttcacgacgc ggacgatctt cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg
    15661 accacggtca tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac
    15721 aatcagagta cgttcaccct gtgccatttt cagcagtgcc gcagccagag tttcgagatc
    15781 cagctcttca ccttctgcag tcggctgaat tttgctcagc agtgcgcggt attgatccag
    15841 atcgctgctt tccagctgct gctgtacttt agcggcgaat ttttccagac ggcgtttgcc
    15901 cagcagttct gcgttcggca gttctacttc cggaatagtc agcttcatag tacgttcaat
    15961 gttgcgcagc agacgacgct cgcggttctc aacgaacagc agcgcgcggc cagcacgacc
    16021 cgcacgaccg gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta
    16081 gttaaccacc aggctgatac gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat
    16141 caggatgtcc agacgaccgt ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt
    16201 catgtcaccg ttcagcgcgg cgctattgta gccgttacgc tcaagagctt cagccacttc
    16261 cagagtcgcg tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag
    16321 gaaacgtacc agtgcttcgt ttttacgcat accccacaca gtccagtagc tctggctgat
    16381 gtcaggacgg gtagtcacgc tggactgaat acgcacttcc tgcggctctt tcataaagcg
    16441 gcgggtaatg cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc
    16501 ttccgggatc tgcgccataa tggtttcaac gtcttcgatg aagcccatgc gcagcatttc
    16561 gtcagcttca tccagaacca gaccgctcag tttagagagg tccagagtgc cacgtttcag
    16621 gtggtccagc agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg
    16681 taattgcacg tcataacgct ggccgccgta cagggcaacc acatttacgc cgcgcatgtg
    16741 tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag
    16801 caccagaatc tgtggtgctt tcagctcagg atcaagattc tgcagcagag gtaaagagaa
    16861 tgctgcagtt tttccgctcc ccgtctgggc catacccaga acgtcgcggc cattcagcag
    16921 atgtggaata cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc
    16981 ttcaaggata ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta
    17041 gtacgtgtgc ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag
    17101 caattttcat tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc
    17161 ctcacccgtt aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat
    17221 tgctggtccg attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat
    17281 gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc
    17341 gtggcgctgt ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca
    17401 ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac
    17461 gtttgttcgc taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta
    17521 tccgattttt cgaagtgctg tttcaatact tctttagcct gcttctcatc gagcttctgc
    17581 tcggcgagat aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc
    17641 agcagatcat cttgcgctaa cttgtcacga ccgccgtaat ataatgcaat cccgcgattc
    17701 aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca
    17761 gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata
    17821 tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga
    17881 ctatcataca acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc
    17941 cgactggcaa ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc
    18001 aatggtaccg cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta
    18061 agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca
    18121 cggttgaatg aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc
    18181 cccgccgcag cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc
    18241 ggtgctgcag caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc
    18301 tggcgatcaa cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc
    18361 actttctcaa cgcgtttgtc agcgatttga gagatgtgta ccagaccttc tttaccgccg
    18421 ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca ctttaccagt gtagacgcgg
    18481 cccacttcga tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg
    18541 ccgtcggtcg ctgcaatctt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt
    18601 tcttcggtca gggcacggat aacagagccg cctttaccga taacgtcttt gatcttgtcc
    18661 gggttgatct tgatggtatg gatacgtggt gcgaactcag agatatcgcc acgcggcgcg
    18721 ttgatcgcct gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagc
    18781 gcaacctgca tgatctcttt ggtgatacct tcaattttga tatccatctg caatgcagag
    18841 ataccctcgc gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc
    18901 aaaatgtcag acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc
    18961 gcaacggcag ctttgatcgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg
    19021 gaagccatag aagaggaacc gttggattca gtgatttcag acacaacacg tacggtgtac
    19081 gggaatttgt ccatatccgg catgactgcc agcacgccgc gcttcgccag acgaccgtga
    19141 ccaatttcac gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg
    19201 aagttgtagt ggaacaggaa ggtatcggta cgttcgccca tcagttcatc aagaacctgc
    19261 gcgtcacgag cagtacccag cgttgcggta accagcgcct gcgtttcacc acgggtgaac
    19321 agcgcagaac cgtgagtacg cggcagcacg ccagtacgca catccagacc acggatcata
    19381 tctttttcac gaccgtcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt
    19441 ttctcgatcg cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc gtcttcagca
    19501 agcagcgtcg cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg
    19561 tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc
    19621 agcgcttcgt ttaccggctc cggctgccag tcccaacgtg gtttaccggc ttctttcacc
    19681 agttcattga tgttctgaat aacaacctgc tgttgttcat gaccgaacac tactgcgccc
    19741 agcatctggt cttcgctcag cagttcagct tcagattcaa ccatcagtac agcggcttca
    19801 gtaccggcaa caaccagatc cagtttgctc tctttcaact cgtcctgagt cgggttcagt
    19861 acgtactggt cattgatgta acctacgcga gcagcaccaa tcggaccatt gaacggaata
    19921 ccagacagag acagtgctgc ggaagcacca atcatcgcga cgatatccgg gttaacttgc
    19981 gggttaacag aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac
    20041 agcggccgaa tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg
    20101 ccttcacgac ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag
    20161 ttaacggtca gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta
    20221 acaaataccg cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc
    20281 atgccggttt ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta
    20341 agcaatgtaa tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc
    20401 gatcttctgc gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc
    20461 ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt
    20521 ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa
    20581 actcgcaaga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    20641 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    20701 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    20761 gcagtcagca gtgctacctg aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac
    20821 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    20881 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    20941 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga
    21001 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcaacgc ggccttcatc
    21061 gtcaatttcg cccataccga taaatttgcc gttctcacct tctgtaacgc gaaccagtcc
    21121 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    21181 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    21241 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    21301 atgttccagg gtcacaatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    21361 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    21421 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    21481 caattcataa acggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    21541 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    21601 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    21661 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    21721 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag
    21781 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    21841 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    21901 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    21961 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    22021 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    22081 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    22141 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    22201 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    22261 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    22321 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    22381 gctgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    22441 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    22501 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    22561 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    22621 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    22681 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    22741 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    22801 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    22861 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat
    22921 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    22981 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    23041 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    23101 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    23161 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    23221 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    23281 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    23341 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    23401 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    23461 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    23521 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga taccagtacc
    23581 cgctttcgca gacacatgta cgaactggct ttcaccgccc cactcttccg gcaggatgcc
    23641 gtactgggag agttcgttct taacgcgatc cggatcggct tctggtttat cgatcttgtt
    23701 cactgcaacg accaccggaa cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg
    23761 cggcattacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    23821 acgagcacgc attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc
    23881 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    23941 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    24001 catgatggtc acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat
    24061 taccgcctct tccagctcgt tttcacgacg caggataact ttatggccca tctcttcagc
    24121 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    24181 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    24241 ggtgatagtt tcgccgatca caacgtcacg gttaacggcc tgagcaggct tctggaagcc
    24301 ttgctgcagc gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc
    24361 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    24421 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    24481 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    24541 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    24601 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    24661 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    24721 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    24781 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    24841 acgggcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24901 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24961 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    25021 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    25081 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    25141 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    25201 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt
    25261 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    25321 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    25381 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    25441 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    25501 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaaac cttcttctac
    25561 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25621 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt tgagttccca
    25681 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25741 gttaccagct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25801 aacgtctgcc ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac
    25861 gatatcgata cgctcgccgc ccagttcagc agaaaccgcc tgaacacgcg cgccacgcat
    25921 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25981 acgagaaccc gggtcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttccgg
    26041 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    26101 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    26161 gaagttttca cgcggcagca tatcttcgcg caggatcacg gcttcagcgt tgttgcccag
    26221 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    26281 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    26341 aacctgtttt gccgtctggg tggtgatacg gtcaaaggta acagactcaa tctgatcttc
    26401 aacgtaatcg cccaagttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    26461 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    26521 gcttttgcga tcgatctgta cacggacgtc gatctcttgc tcatattttt tctttgttgc
    26581 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26641 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26701 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26761 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26821 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26881 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26941 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    27001 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    27061 tgtgcgaccg cgaataaatt cgatgcctac cagttcaaaa cccagggcct caactggcgc
    27121 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    27181 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    27241 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    27301 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    27361 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    27421 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    27481 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27541 gtgatccatg ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccagttc
    27601 actttcattt gttgaatact tttgccttcc cctgctctcc cttaagcgca ttattttaca
    27661 aaaaacacac taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat
    27721 tttgcataaa aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg
    27781 tgttatttta tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct
    27841 ttttccggcg gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt
    27901 ccttatgcat atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct
    27961 cgtcgtgcca tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg
    28021 gtggccgaag gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg
    28081 acctatttca acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg
    28141 atgaaagaag atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc
    28201 gaacgtttct atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg
    28261 cttgatactg actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt
    28321 gcctgcggtt tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg
    28381 cttggtgcaa cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc
    28441 aacccgatta tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc
    28501 acagtacgct ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac
    28561 gtagaaatga tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac
    28621 cagattgaga accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg
    28681 gcactgctgc acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt
    28741 gagcagtatc acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt
    28801 gactcccagg cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact
    28861 ggtgaagtta ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca
    28921 gagaacctga cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg
    28981 ccagatgatc gtattggtca attgaccatg cgtaatctgg atatcactga tacccgagag
    29041 aaactttttg gttatgccaa aactggcctg ctttcctcct ctgccacttc aggcctgccg
    29101 caggtggaga atatggaaaa caaaggccag taattcgaca tcaaccctgc ccttcagggg
    29161 tggggttacc ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat
    29221 catggtttgc atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg
    29281 tttgcggctg ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag
    29341 ctgtcgaaaa gatattgttt tccgttgtgc gatccacgcc atccccaaga acggggctat
    29401 accagataaa catcgggaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat
    29461 agacgttctt cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg
    29521 cgcggcgatc ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg
    29581 agttgtcata gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat
    29641 aggcgctaca agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct
    29701 gcgtattttg ctgtaatgct tgcgataaat gcggcaacaa caattcatca aatcctctga
    29761 cataaactgt ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa
    29821 aagcggattg cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa
    29881 tgttgtcggg gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg
    29941 gaaccgacag cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt
    30001 ttctttgtgc ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt
    30061 cgacacgtac agactccccc acaatcaaca cgtaggtatc aatacttgta tccctgactg
    30121 ataattgaaa atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc gctttcgctg
    30181 ctaaagcaaa atagttgaga ttgaaaaacg gcgtataggt agcaaatcac gacgctagta
    30241 tatatggact gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa
    30301 ataaactgcc cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg
    30361 aaacatcata ttttattatt actgccaaaa aaagaaggga taaaaaagca aaagcacata
    30421 gataagggat atacatcccc agcattttca caacttcatc cggatcactc tgcagcacac
    30481 taatcgcaaa tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac
    30541 tcatgtctgc cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg
    30601 atgatatacg tttaacaacc aggatggtca ggacgaagaa cagcaatcgc tgaaccatat
    30661 gcgctccgga agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca
    30721 accaatgaga tttaaaatct tctagcaaat ttgttgaata ctgtcatcca gaaatccaag
    30781 ttcacaacca atataaacaa ttatcaacaa tgttcaattt tatatcagac atgtttttca
    30841 actctgatcc atatcagacc gaaacactgt tagttttttc tttttaaatg atgcgaccat
    30901 tgcgttctca gtaattaaat aacattatca atgcgtctct aaacattacg cagaacgtag
    30961 ccatcagtag ttatactgac aggagcagga tggtttatcg agaagtaaac atatccgtat
    31021 tcgtaccgga actggagttg acacaataaa gtgccaatta tgtcagcagt agggaaaagt
    31081 ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc agctacgagt aaagcaactg
    31141 gacgagatac agatacctga caaccattca tcagaccagg accaaaacga aaagagacgc
    31201 ttttcagcgt ctcttttctg gaatttggta ccgaggacgg gacttgaacc cgtaagccct
    31261 attgggcact accacctcaa ggtagcgtgt ctaccaattc caccacctcg gcacggatac
    31321 tactttttag ttcgggatat cgctggtcgg cttagccgga gcagctggct gagtttgttc
    31381 ggttttcgcc ggtgcactca gattttccca ttcgctacct ctattggttt tgttgctatt
    31441 gatgttaccc agcaccagac tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg
    31501 ggtcatgaag ttaccagaac cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa
    31561 ggaggctccc atatcagcgc ctttaccttg ctgcagcatg atcagaccaa caaggccaat
    31621 tgccacaata aggaaaacta ctaaaagagc ttcgtacata atcaacctgt tccttgcgga
    31681 gttgccgcgt accaatgctt caaccaataa agcgggagtt tttaatcttt cccactgaag
    31741 cgggtgtgaa tactaaccaa aacgaatgac cttcgcaagg gcaattttat cgcattgtat
    31801 caactgcgga aaaaaacagc aaaaccgatg tgttcgccga gaaaaaaggc gacaatcgcc
    31861 gcctttttag ccagttatct aacgctttaa acggctttta ctgcatcggc gatgcggtgt
    31921 gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat taacggttcg
    31981 gtgccggatt tacgcagcaa cacgcgtcca cggttgccca gcgcagcttc aacctctgcg
    32041 gtcacggctt taactgactc atgctcaagc ggatcgccgc tacctgcggt gtaacgtacg
    32101 ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg
    32161 ttacgtgcca tcgccgccag cacctgcaag ccagcaacga tgccgtcacc ggtagtggtt
    32221 ttatccagca ggatcacatg accggaattc tctgcaccga tacgccagcc tttctcctgc
    32281 attttttcca gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat tcctaactgt
    32341 ttcagcgcca gttcaagccc catgttgctc atcaatgtac ccacagcacc accacgcagc
    32401 tggccctgac gaagaccttc acgcgcgatg atatacatga tctgatcgcc atcgacttta
    32461 ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc aataccgaga
    32521 tccgcttttt cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg
    32581 gcattgatgt ttacaccgtt tggctcacaa ccgatagcga taacgttcgc ccccagttcg
    32641 cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg cacaatccac cacaatcttc
    32701 agttcactga ggctaagttc gttcgggaac gtggctttgc aaaactcgat atagcgaccc
    32761 gcggcatcaa cgatacggct ggctttaccc agttcagccg aatcaacgca gctgatctcc
    32821 ttttccattt ccgcttcgat ggcctcttct accgcatccg gcagtttagt gccgtcgata
    32881 gagaagaatt taatcccatt atcgtagaac gggttatgcg atgcagatat cacaattccg
    32941 gcctctgcgc ggaaggtacg cgtcagataa gccacggccg gtgttggcat cgggccagtg
    33001 aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca gtgctgactc cagcatatag
    33061 ccagaaatac gcgtgtcttt accaataata atcttacggg agccgtggcg cgccagcact
    33121 ttacccgcgg cccaacccag cttaagcaca aaatcaggtg tgatcggcgc atcccctaca
    33181 cgaccacgaa tcccatcggt accgaaatat ttacgattac tcatagcgtt tgttttcctt
    33241 tgcagacaga gtggcttcca ccacccgcat cgcttctacg gtttctttga cgtcatgaac
    33301 acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca caggccagac taccgctcag
    33361 gcgctcggac ggccccacgt tcagcagctg cccaatcatc gattttcgtg acatacccac
    33421 caacagcggc aggttgaaat ggtgaaattc agccaggcgc gccagtaatg aatagttatg
    33481 ggagagattt ttaccgaaac cgaatccggg gtcgagcaac aatttctctt ttgcgatacc
    33541 cgcctgctcg caacgtgcta tttgctcaat aaagtagcga ttcacttctg caaagacatc
    33601 gtcatacttc ggagcttcct gcatggtttt tggatttccc tgcatatgca tcagacaaac
    33661 cggtaaaccg gtttctgcag ccgcctccag agcgccaggt tcggaaaggg agcggatatc
    33721 attaataatg tgagcgccaa ctttcgctga ctcacggatg acttctggtt tggatgtatc
    33781 gaccgagatc cagacttcga agcgttgagc aattgcctca accacaggaa taacacgttg
    33841 caactcttct tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc caccaacgtc
    33901 aatgatcgtc gcgccagcat tgatcatcag attcgcatgt ttcaccgcat ctatcagcga
    33961 gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg
    34021 aggatggcta aggtccagtg aagtaccctg ggcaaagagt ttcattatgt tatccctggt
    34081 atgaattgat aagaaaaaac cccggagcac gccccggggt tttcggtaca aatacagtca
    34141 tctgatgcgg gaacttactt gtcgcctaac tgctctgaca tggtgttacc cgggttcggc
    34201 gtacgcggtt catcaaccgg acgaggagcc tttggactac cattgtcgcc agaattgtta
    34261 gaagcgcctg gttcttccca gcccgctggc ggacgtacat cgcgacgtgc catcaggtca
    34321 tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag catctttcat cgcatgcaga
    34381 atatccatat tgtcggtcag aagctgacgc gcacgattat agttacgctc aatcagtgct
    34441 ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg
    34501 ctacggccga ggaatacttc accttcttct tccgcgtaca gcagtggacc caatttctca
    34561 gagaagcccc actgagtcac catgttacgt gccaggttgg tcgcaacttt aatatcgttg
    34621 gacgcaccgg tagaaacatg ttccggcccg tagatgatct cttctgccag acgaccaccg
    34681 tacagcgtag aaatctggct ttccagtttc tgacggctgg cgctgattgc gtcgccctca
    34741 ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga taatcgtcac tttatgcacc
    34801 ggatcgtgtt ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtt
    34861 gattctttct gcgcttccgt catcaccatg gagcgacgtt ccgcacccat catgattttg
    34921 tctttcgctt tctcgaactc aaccatcgac acaacgcgtt tgttgccacg agcagcgaac
    34981 agtgccgctt cgttcaccaa gttcgccagg tcagcaccgg agaaaccagg agtaccacga
    35041 gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg aactttcagg
    35101 atctgctcac gaccgcgaac atctggcaag ccgaccacaa cctggcggtc gaaacggcca
    35161 ggacgcagca gggccgggtc gagaacgtcc ggacggttag tcgcggcgat aacgatgata
    35221 ccttcgttac cttcgaagcc atccatctca accagcatct ggttcagagt ctgttcacgt
    35281 tcatcgtgac caccgcccag accagcgcca cgctggcggc ctacggcgtc gatttcatcg
    35341 ataaagatga tgcacggtgc cgctttcttc gcctgttcga acatgtcacg aacacgggat
    35401 gcacccacac cgacgaacat ttctacgaag tcagaaccgg agatagtaaa gaacggaact
    35461 ttcgcttcgc ctgcaatcgc tttcgccagc aacgttttac cggtacccgg aggaccgacc
    35521 atcaggacgc ctttcgggat cttaccgccg agtttctgga agcggctcgg ctcgcgcaga
    35581 tactctacca gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag
    35641 gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca
    35701 cctttgccac cgccgccctg catttgacgc atgaagaaga tccagacacc aatcagcaac
    35761 agcatcggga accaggagat gaagatagaa gccagcaggc ttggttcttc aggcggttca
    35821 ccgacaacct tgacgttctt ggtcaacagg ttatccagta atttcggatc ctgaaccgga
    35881 atgtaagtgg tataacggtt actatctttc ttggtaacgt tgatttcacg tccgttgata
    35941 cgcgcttcac gaacctggtc gttattgacc tcttgtagga aggtagagta atccacctta
    36001 cggccattag actcgctggg cccaaagctc tggaatactg acatcagcac aacggcaatg
    36061 accagccaga gtattaggtt tttcgccatg tcactcaagg gattaacctc ttattacaac
    36121 tgtgttaaaa acagcgtcag gatactctat atccagcatc tttcaaactt tcgtctgaaa
    36181 tctcccggtt agggtttacg cccggtcgct acaatataca cttctcgcga acgtgcacga
    36241 gaagagtccg gcttacgaac tttgaccttc gtaaacaggg agcgaatttc ccttagatac
    36301 tcatcgaaac cttcgccctg gaacaccttc actacaaaac tgccacctgg cgctaataca
    36361 tcacgacaca tttctagcgc cagttccacc agatacatgg ctcgggggat atccaccgcc
    36421 ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa cctggacttt gctgtcgcca
    36481 acgcgctcca gcagtgcttt catcaccagt tcatcacgaa aatcgccctg aagaaagtcc
    36541 acaccaacga taggatccat aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca
    36601 atttgggtga ccacatattg tgaccaacca cccggagcag caccaaggtc gacaaccgtc
    36661 attcccggtt taaagagttt gtcactttgc tgtatttcat caagtttaaa ccaggcacgg
    36721 gaacgtaatc cctttttctg tgcctgttga acatatttat cgctaaagtg ttcctgaagc
    36781 cagcggctgg agctggcaga acgcttctta cctgtcattt aactttccca tggggataac
    36841 tcatcgtaac caattgcgta aatttttacg cgcctatttg gtgatatatg ggagatggcg
    36901 gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc tgagtactaa
    36961 acaaaaacag cacctgaaag gtctggcaca tccgctcaag ccagttgttc tgcttggcag
    37021 taatggtttg accgaagggg tgctggccga gattgaacaa gcgttagagc accatgaact
    37081 catcaaggtg aaaatcgcca ccgaagatcg cgaaacaaaa accttgatcg tggaagctat
    37141 cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg
    37201 cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag ttacacacat
    37261 ttgctgtgta aaacgagggg ttttccgcag gcaggagagc aaaatgccac gctctgttcg
    37321 ttgataaaag gccgcagagc ggcctttttc ctttctttac aatacatcaa catcttgagt
    37381 attgggtaat tcttacaggt attccacctt aattacttca aattctactt cgccgcccgg
    37441 cgttttgatg accacaacat catcttcttc tttgccgatc aggccacgag caataggcga
    37501 gtttacagaa atcaggtttt gtttaaagtc agcttcgtca tcgccaacga tgcgataagt
    37561 ctgttcttcg tcagaatcca gattcagcac cgttacggta gcaccaaaaa taacgcgccc
    37621 attgttgggc attttggtga catcaatcac ctgcgcgttt gacagcttgg cttcgatgtc
    37681 tttgatacgg ccttcgcaga agccctgctg ttcacgagct gcgtggtatt cggcgttttc
    37741 tttcaggtcg ccatgctcac gcgcttccgc gatagcagca atgatttcag gacggcgcac
    37801 agatttcaga aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat
    37861 agcttgcatt tgttatacct cttgaatatt cctgatgggg caagtcttca cccaccccgg
    37921 ctgttcggcc tgcccggcat aactcctcac cgggaccaga agcaaaaaaa taccgacccg
    37981 ggtacaagtc ccaggtcagc tacaattcac attttgatgg tcattttacc ctgaagttcc
    38041 cgaagggtca tcgtttactt tatagggcgt tgcgccgtag tatgacggct cgattccagg
    38101 ttgttagcgc gagattatgc gattttccag atttatcatc ggattgacca gctgtatagc
    38161 gttcagtgtt caggccgcga atgttgatga gtacgttact caactccccg caggtgccaa
    38221 ccttgccctg atggtgcaaa aagtcggtgc gtcggccccc gctattgatt accacagtca
    38281 gcagatggcg ctgcccgcca gtacccagaa agtgattact gcgctggcgg cgttgattca
    38341 actcggcccc gattttcgtt tcaccacgac gcttgaaacc aaaggcaatg tggaaaacgg
    38401 cgtacttaag ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa aacgtcagga
    38461 tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg atggcaatgt
    38521 gttgatagat acctccattt tcgccagcca cgataaagcc cccggctggc catggaatga
    38581 catgacacaa tgctttagcg ctccgcctgc tgccgccata gttgaccgca actgtttctc
    38641 cgtctcgctc tacagtgccc caaagcctgg tgatatggct tttatacgcg tggcatctta
    38701 ttaccccgtt acgatgttca gccaggtacg caccctcccc cgtggttctg ccgaagcgca
    38761 atactgcgaa ctggatgtgg tgccaagcga cctgaaccgc tttacgctga cgggatgcct
    38821 gccacaacgt tctgagccgc tcccgttggc ttttgccgtg caggatggag ccagctatgc
    38881 cggtgcaatt ctgaaagatg agttaaaaca ggcgggtatc acctggagcg gaacactgct
    38941 gcgccagact caggttaacg aacctggaac ggtagttgcc agtaaacagt cggccccgct
    39001 gcacgatctg cttaagatta tgctgaaaaa gtcggacaac atgatcgccg atacggtttt
    39061 ccgcatgatc ggccatgcgc gcttcaatgt gcctggaaca tggcgggctg ggtcggacgc
    39121 cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt ggtaacacca ttattgccga
    39181 tggttcaggg ctttcgcggc ataacctgat tgcccccgcc accatgatgc aggtgctgca
    39241 atacattgcc caacacgaca atgaacttaa ctttatctcc atgctgccgc ttgcgggcta
    39301 tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg gcaaagtctc
    39361 agcgaaaacc ggttcgttgc agggggtata taacctggcg ggattcatta ccacagcgag
    39421 cgggcaacga atggcatttg tacaatatct ttctggctat gcagtaaaac ccgcggatca
    39481 gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt ttgtacaaag atatttatca
    39541 gaacaattag tcaaaaagaa accccggcgc atggccgggg tttcagatta ttgacaaagt
    39601 gcgctttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcgtgcaaa
    39661 ttcaatatat tgcaatcatc ttgtaggcct gataagtgta gcgcatcagg cttatttggc
    39721 attggtcatt agtgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc
    39781 ccagtcgtca tcccagtctt catcatcctc ttcagcaatc tcttcaagct gctgacggtg
    39841 ataatcatcc cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac
    39901 cgggttttca atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccgct
    39961 cgccgcagag atcagataat atttatcttc ccagcccagc gcttcagcga tcgctttcgc
    40021 tttctcttcg gcttctgcct tatccagcag atcgatcttg ttgaagacta accaacgcgg
    40081 tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc
    40141 aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg
    40201 ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag
    40261 tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact
    40321 tggcaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg
    40381 aataaaggtc gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat
    40441 cagctccagc agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt
    40501 acggttaact gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac
    40561 cagcagacgc tgaccgtgtt tagtcatatc acccatggtt tcaccagtac cctggtcgat
    40621 tacacgcgta cctaccggca ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc
    40681 gcggcttgcg ccgttctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat
    40741 aagcgtgttc aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc
    40801 gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc
    40861 gccatcacct gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt
    40921 aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca
    40981 aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata
    41041 acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc
    41101 tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg
    41161 ccagcgagcc atcgcttccg ccaaggcgcc atatcctacc aaggtattca gtccgcaaaa
    41221 aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg
    41281 gaagagcgcg attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg
    41341 acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc
    41401 aaagataact ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt
    41461 aaaaaacatc accagaccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat
    41521 tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat
    41581 cacctgcgaa gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc
    41641 aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg
    41701 taatggcggc aacctcttct tcaccgcaag aatggcacca aggccaatac tcgccatcaa
    41761 gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat
    41821 tggcaacgcc ccccagcaaa ttgctgtggt aagcgccaaa agaatgccaa tgcctgcctg
    41881 ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat
    41941 ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac
    42001 cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt
    42061 aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc
    42121 tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag
    42181 aacggattcg ccaccgaaac gcttaacgcc caagcgttta gcttctgaat cgcgaccgtt
    42241 acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg
    42301 ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag
    42361 tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact
    42421 tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt
    42481 gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc
    42541 aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg
    42601 tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata
    42661 ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat
    42721 acacgaagaa aaaaacacaa cttgtacggt aacgtttatc tgtgccattt tttcagtaca
    42781 attaccctat attcctaacc ataaacccta agttgccttt gttcacagta aggtaatcgg
    42841 ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga
    42901 tatggcgggt gttaatgcgg caatccttga gcagcttaat tccgacgtcc aactgatcaa
    42961 tcagttaggc tattacatcg tcagcggcgg cggtaaacgt attcgtccga tgattgctgt
    43021 actggctgca cgagctgttg gctatgaggg aaatgcgcat gtcaccattg ctgccctgat
    43081 cgagtttatc cacacggcga ctctgctaca cgacgacgtt gtggatgaat cagatatgcg
    43141 caggggtaaa gctaccgcca acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga
    43201 ttttatttat acccgcgcgt tccagatgat gaccagcctc ggttcactca aagtgctgga
    43261 agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt
    43321 taacgatccg gacatcactg aagaaaacta catgcgcgtt atctatagca aaaccgcgcg
    43381 tctgtttgag gctgccgcgc agtgttccgg gattctggct ggctgtacgc cggaggagga
    43441 gaaaggcctg caggattatg ggcgctatct cggcactgct ttccagttga tcgacgattt
    43501 actcgattac aatgccgatg gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga
    43561 aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat ggcacaccag aacaggcaca
    43621 gatgatccgt accgccatcg aacaaggtaa cggtcgccat cttctggaac cggttctgga
    43681 agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga
    43741 caaagccatc gcagcgttac aggtgctccc ggacacccct tggcgagaag cactcatcgg
    43801 cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc
    43861 gcgggatgat tccagtaaat tccataaaaa cactaattca gcgctataac acctgaaaaa
    43921 agggcgatga atctttatat attctgaata ttcacactct ttacaggaac tttttagcgc
    43981 aatagaccat caggagtata gtgatgcccg acagaagaag tgttctgaat gaaagcgact
    44041 aacttaagga gtgaggaaag atggaaagta atttcattga ctggcatccc gctgacatca
    44101 ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga atctcgcaga aatggtttga
    44161 gttcctcaac gctggcaaac gcattatcgc gcccatggcc gaaaggagag atgattattg
    44221 cgaaagccct gggaactgac ccctgggtta tctggccatc acgctaccat gatccgcaga
    44281 cccatgagtt tatcgacaga acgcagttga tgcgtagcta cactaaaccg aaaaaatgag
    44341 tggtctggcg gtagccccgc gaacggggct gccagctctc agacgattac tcgcctttca
    44401 cacgctcaat atttgcacct aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat
    44461 cgatgtgata aatacgatca accaccgtcg tcccttccgc aatacagcca gccagcacca
    44521 ggcttgctga tgcacgcaga tcggttgcca taacctgtgc accagaaagt ttttcaacac
    44581 catgacaaat aacggtattg ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg
    44641 gcacatgcat aaagcggttt tcaaagaccg tttcggtgat gaacccggtc ccttcagcca
    44701 ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg
    44761 tacgtacgtt aacagccttc ggacgtttgc catgcatatc caggctaatc cagtcttcgc
    44821 cgacttcgat gtccgctcca gcatcacgca gtttcgccag cacggcgtcc agagtatctg
    44881 gctgcgcgtt acggcagata attttgccac gagagatcgc cgccgccacc aggaaagtac
    44941 cggtttcgat acgatccggc agcacgcgat agacaccgcc gcctaaacgt tccacacctt
    45001 cgatgacgat acgatcggtg ccctgaccgc taattttcgc acccagcgta atcaggaagt
    45061 tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg
    45121 ccagggttgc agcacacatg atggtcaccg ttgcgccaac gctgacttta tccatcacga
    45181 tatgtgcgcc tttcagacga ccatcgacgg aagctttaac gtaaccttct tccagtttga
    45241 tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc aaccggacgc gcaccgatcg
    45301 tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg cgctaccagc ggccccagcg
    45361 cccagataga agcacgcatg gttttaacca gatcgtaagg tgcgcagaat acattaacgt
    45421 cgcgggcatc aatatgcaca gaaccattac gttctacttt cgcacccagc tggcttagca
    45481 gtttcattga tgtatcgacg tctttcagtt tcgggacgtt ctggatctct accggttctt
    45541 ccgccagtag tgcggcaaaa agaataggca gagcagcatt tttagcgccg gaaattgtga
    45601 cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt atccatttag tttgttctca
    45661 gttaacaatt catatccgct accggcgaat cgcccatagc tcaaaagccg ttcagtttgc
    45721 gatcgcgcgc ccactccgca ggggtatacg ctttgatcga cacagcatga atgcggttat
    45781 ccgcaatata ttccatcagc gggccataga ccgtctgctg ttttttaacc cgactcatac
    45841 cgtcaaacaa ctcacccacg gcaataacct gaaagtggct gccatcgccg gaaacgtgga
    45901 cttcctggag ggagagagcg ttcatcaaca cgctctgaat ttcattattt tccatgggat
    45961 cttcaatcat cagttaataa accagcgaaa catcttagag caaagttgcg ctggcataaa
    46021 taagcaaaaa gcctcgctga taaatcagac aaggctcgac ttgcaggcag gtttgccgga
    46081 caggcggtta acgccatatc cggcctgaaa aaatttaacg aggcagaaca tcagcaggca
    46141 aattatacaa tttcgcgagg gtatacactt tgtcgtttac cccctgaagc gtcacgttgt
    46201 tgccctgctt tttcgccaga tcgataagat ggagcagcag tgccagtccc cccgtatcca
    46261 cgcgggacac acggctaaga tcgatgcagg taatcccctt caccgcctcc tcacgcattt
    46321 cccaaagcgg tagcagaacg tcctgatcca gctctccgga taacgccagc gtgtcacccg
    46381 tctgcatcca gctcagtgac tcgctcatta ttttttctct tccagagtga ttttctgttg
    46441 agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc
    46501 ccactcgttt tgtttggtgg tgatcatact gacgccttca gcaatcatgt cgtaagcttg
    46561 ccagtttccc gtctgggagt ttttacgcca ctggaagtcc agacgcaccg gcggacggcc
    46621 attcgggtca ataatggtaa cgcgaatagg cacaatggtt ttatcgccca gcggctgttc
    46681 tggcgcaatc tgataggttt gaccgtgata catcgccagc gcctgaccgt aagcctgctt
    46741 caggtactca cggaaagcgg caaagtaggc ttcacgttga gcaggggtcg cactcttgta
    46801 atactggccc agcaccagcg caccggcgta tttcacctgt acgtatggca gcagttcctg
    46861 atcaacaatg gtacgcagat aatccgggtt ggcccgaatt tgcggttgct cattcttcag
    46921 gcgatcgaac gttttctgcg ccgcctcgtc catcagctta taaggattgg tctggtctgc
    46981 cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg accatcatta aacgtttaaa
    47041 catgcgtcgg ttctcctgaa attatttcgt tgtacccaca ggttcagtgg tttcattatt
    47101 acctggcgca gcagctggcg catcgccact attcttattg tcatcgcctt tactaccgta
    47161 aaggaactga ccaatgagat cttccagcac catcgcagac ttggtgtcct gaattgtatc
    47221 gccatccttc aggatagcag tccccagttc cgggtcttca aaaccgacgt ttaatgccag
    47281 atattgttct cccagcaggc cggaagtacg aatgctcagc gaactggtat caggaatgtg
    47341 gttataacgt tgttcaattt ccagcgttac gcgcggcaga taggttttcg ggtccagcgt
    47401 aatatccgcc acccggccca caacaacacc accaatgctg accggagagc gcgctttcag
    47461 gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc gattcagtac gtatggacgt
    47521 cacgttcgcc gccttcaggc aaacaaacag cgccgccagc agtgctgcta ataaaaagat
    47581 acccacccaa atttcatttt ttttcgtttg catgaactca attcccaaac atcaatgcgg
    47641 tcagcacaaa atccagcccc agaacagcca gagacgagtg gacaacggtg cgagtggttg
    47701 cccggctaat cccggcagac gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg
    47761 tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt gaccagatcc atacgccagt
    47821 cgacggcatt ttgcattgcc gaccagaaga acccgctatc aatgcctttc caactgacgc
    47881 cgaccagcga tccgccccag atccccacgg cgacgaagat aaccgtcaac agtggtaatg
    47941 aaataacccc agcccagaaa cggggagaaa taacccgacg cagcggatcc accgccatca
    48001 tctccatact ggagagttgc tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg
    48061 aaccagcacg cccggcaaac aacagcgcgg caaccaccgg ccccagttca cgcagtagcg
    48121 ataacgccac cagcataccc agactggttt ccgcgctata agtggtcaga accagataac
    48181 cttgcagccc caacaccatt ccgatgaaca cgccagaaac tacaataatc agcatcgaca
    48241 ggacgccgac attatagagc tggcgcacca gcagcggcgc atgtttgcga aattccggtt
    48301 tgccgaccag cgcattgaat aacattaacc cggcccgccc gaacgttctc agggttttaa
    48361 tccctttatg tccgagcgac gccagcgcat ttaacagcat gagtggctta actccctggt
    48421 aaaagatcag cgtgataatc gccggcagga tagcggaacg gaacaggccc gtcagctatc
    48481 ccgtccagaa actgacgtac gcgcggatca ggattcgtct gcaacgcctg ggcactgcca
    48541 tgagcgacaa tttttttgtc cgccaggatc caggcgtgat ccgcaatact taacacttcc
    48601 ggcacatcgt gagaaaccac cacacaagtc acgcccagcg cgctgttcag ctcagaaatc
    48661 agcttcacca gtacgcccat ggtgatggga tcttgcccaa caaaaggttc atcaaacatg
    48721 atgagatccg gctccagcgc aatcgcacgc gccagcgctg cacgccgcgc catcccaccg
    48781 gaaagttcag aaggcattag tttagccgct ccacgcagcc ccacggcctc cagcttcatc
    48841 atcaccgtac tatgcaacaa tggcgcggga agttgggtat gttcgcgcag tggataggcg
    48901 acgttgtcaa atacgttcat atcagtgaac aacgccccgg actgaaataa catgctcatc
    48961 cgtttacgca ctgtatacag gcgcgaacga gacatcgccg gaatattctc accatcgaaa
    49021 aggatctcac catgatctgg tgcgatttgc ccgccaatca gacggagtag cgtcgtttta
    49081 ccgatgcccg atggccccat gatcgccgtg atcttccctc gcggcacggt cagggaaata
    49141 ttatcgaaga tgcagcgatt gccacgcgta aaactgacat cgcgcatatc gactaaattc
    49201 gccacagact gctccataat tcacccttcg tcttgcgttg attttctaag catggcgctc
    49261 aatttaacct tgaacccaac atatttacag aatattaccc gccgtggtta gcgaaagctg
    49321 gcatttgttt tactttttag ccgcataaag tcaaaattaa gaatccgtta cggctttctg
    49381 aaaatcttca gcggaccggc gagtatacct gaagaaagga cgttagatgc ttttagctac
    49441 ggcactgtta attgttggtt tacttttggt cgtttacagt gccgaccgcc tggtttttgc
    49501 cgcgtctatt ctttgccgaa cctttggcat cccgccgctg atcatcggca tgacggtggt
    49561 cagtattggt acatcgttac cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca
    49621 acgcgattta gccgttggta cagccctcgg ctcaaacatt atcaatatat tgctgatcct
    49681 cggtctggct gcgctggttc gtccttttac cgtccattcc gatgttctgc gccgtgaatt
    49741 acccttaatg ttgttggtca gcgtggtggc cggttccgta ctctatgacg gacaacttag
    49801 tcgaagcgat ggtatctttc tcctctttct ggctgtgcta tggctgctgt tcattgttaa
    49861 acttgcacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    49921 cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc
    49981 cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    50041 gacgatggct ctgacggcaa ttgctatcgg aaccagtctg ccggaactgg caaccgcaat
    50101 agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat
    50161 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag agattgatcc
    50221 actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    50281 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    50341 tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    50401 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    50461 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    50521 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    50581 aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat
    50641 ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg
    50701 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    50761 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    50821 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    50881 caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    50941 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    51001 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    51061 ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta
    51121 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    51181 gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    51241 cggggggaat acgtgtgcgc cctggcattc tggccgttga ggcactgaac ttaatgcagt
    51301 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    51361 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    51421 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc
    51481 agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta
    51541 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    51601 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    51661 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    51721 gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt
    51781 tggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    51841 cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg
    51901 tggcgcagtg cgcgaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    51961 caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct atcactggcg
    52021 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    52081 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    52141 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    52201 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    52261 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    52321 gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa tcacgacgga taacgcgcag
    52381 atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    52441 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    52501 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    52561 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    52621 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    52681 taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    52741 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggatacggta aaccggcaac
    52801 gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc agatgcacta
    52861 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    52921 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    52981 cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa
    53041 caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    53101 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    53161 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    53221 ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    53281 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga
    53341 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    53401 tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca
    53461 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    53521 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc
    53581 cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgaacacc tgcgcgacag
    53641 cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    53701 cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag aaatcttaca
    53761 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    53821 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    53881 cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct gttgcagttg
    53941 tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag
    54001 caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga aacgctggac
    54061 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    54121 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgacgag
    54181 ctgccggtct accagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc
    54241 gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt
    54301 gatgaaaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    54361 gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgacccggtc
    54421 ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataag
    54481 accacgccgt ggctggaaga ggccagattg atcattagcg atcatctcga tctgttagcc
    54541 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    54601 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    54661 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa
    54721 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    54781 aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg
    54841 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    54901 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    54961 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    55021 tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat
    55081 accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cactggtgaa gaaattaatc
    55141 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    55201 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    55261 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    55321 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttacagctaa
    55381 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    55441 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    55501 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    55561 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    55621 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg
    55681 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    55741 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact gtcagagcaa
    55801 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tgccgcctca
    55861 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    55921 tattgccatt ccgcatggca aacttgaaga agatactctg cgtgccgttg gcgttttcgt
    55981 tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    56041 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    56101 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    56161 gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa
    56221 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    56281 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    56341 ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg atcgcgagat
    56401 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    56461 ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg atgccgaccg
    56521 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    56581 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    56641 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg
    56701 tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt
    56761 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    56821 gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    56881 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    56941 gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg gttgtaccgg
    57001 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    57061 taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    57121 actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    57181 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa
    57241 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    57301 gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct ctttaattct
    57361 ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa gctcccccga
    57421 caataagaaa atcacgtact gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg
    57481 cttttcactt tcagaagaac cttaagaaaa ccttaagagg cattgtttag gttttgttta
    57541 agttaatcga ccatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct
    57601 tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag
    57661 atgcccaacg ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc
    57721 gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac
    57781 cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg
    57841 ccgattttga cggcaagcct tccattacgc tgaccgaatt tgctgaacaa tgccgttacg
    57901 aagaagacat tgcacaactg cgccagctac tgaaacagct gaagcgttat ttgcaggata
    57961 accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt
    58021 cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga
    58081 cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc
    58141 aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg
    58201 ccctcacttc gcgcgaagct taatccagct ggtgttgttg cataaacggc tcaccgccta
    58261 actgatacat ctgccgtaaa atccacgcct gacggctacg cacgtagccc gatggcgagg
    58321 agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg
    58381 taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac
    58441 cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg
    58501 tttctatccc cagcgttaat ccagcttcca gcccttttcg tacccagcta cgcccatccc
    58561 ataaaaagag attttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc
    58621 attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc
    58681 aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc
    58741 catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac
    58801 ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc
    58861 cccgccccag aaaacggcga ggacaaccat taaccgcaaa agaaaacggc gaacgaaact
    58921 aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta
    58981 tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca
    59041 atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc
    59101 atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa
    59161 tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc
    59221 tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc gctaccaaga
    59281 ctggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc
    59341 agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct
    59401 tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca
    59461 acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac
    59521 aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt
    59581 acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt
    59641 taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt
    59701 gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata
    59761 atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag
    59821 ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg
    59881 ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc
    59941 aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc
    60001 acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga
    60061 ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat
    60121 cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc
    60181 tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa
    60241 tttcttgatc atcgcggtta gcgccggaac cgaaagcggc ttactcagca catcatccat
    60301 tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg
    60361 cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat
    60421 atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa acatctccag
    60481 cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg
    60541 cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg
    60601 catatcgtct tcatcaaacg catcatcgac ctcttctgcc accgacggtg cgtggatcgt
    60661 caacgtaaag gttgaacctt tgccctgttc actggtaacc gtaatatcgc cgcccatatt
    60721 tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc
    60781 atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat
    60841 gccgatacca gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg
    60901 cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat
    60961 ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg taggctccag
    61021 gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag
    61081 gaagctggtg aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc
    61141 catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatagtttt
    61201 gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcaggccgac
    61261 gataccgttc agcggtgtac gcaattcgtg actgatggtg gaaataaacg tcgttttgtc
    61321 gcggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa
    61381 gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa
    61441 gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg
    61501 acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc
    61561 agcaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg
    61621 gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaagtcgg gtgaagcatc
    61681 aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc
    61741 tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg
    61801 aacggcaatt tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa
    61861 atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc
    61921 ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc
    61981 aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac
    62041 cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg
    62101 caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt
    62161 cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt
    62221 ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac
    62281 ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc
    62341 attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg
    62401 gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg
    62461 agcgtataat cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata
    62521 atgctgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgct ggtttcaacc
    62581 actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc gacaattaag
    62641 tgggaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg ataacaggca
    62701 aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc
    62761 tccagccaca cttcgtaacc ctggtcctta tattcgcaaa gcagatccag caccgcatcc
    62821 ggcacgcagt ccgggcgggt accaacacac aaaccgacaa tattggcctg gctcaccgcc
    62881 tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag
    62941 taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca
    63001 atggaacgat gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagcca
    63061 ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta
    63121 tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt
    63181 aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc
    63241 aattcggcga tgacctggat caatcgtccc ggcctgcttt tatcaactgc ataatcaatc
    63301 aaaattaccg aaatttcatg cacaatcaca taaatcactt ttgcttatct tgtgtcagat
    63361 ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta
    63421 aggcagtata aaatgctggt tttctcgtca gttcaaggca ggataagggt taacacgcct
    63481 ttatgacagt caggaattga ctgtttctct aacgactctc cttttagcct taaagataaa
    63541 atctatttta atttcagcta tttaataaag aattttgcgc taaagcacat ttctgtacca
    63601 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
    63661 ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt
    63721 attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa
    63781 accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca
    63841 gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
    63901 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
    63961 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga
    64021 cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg
    64081 cggctggcgt ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga
    64141 actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat
    64201 tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc
    64261 tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cacgcgatat
    64321 ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga
    64381 cttctacgtc tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc
    64441 ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct
    64501 gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg
    64561 ctatctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc
    64621 gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccattcgt
    64681 caacgaaacc ggttctgact ccagttccat ggataacatg ctggaactgc tgctggcagg
    64741 cgggatggat atcatccgcg ccatgcgcct gttagtgcca cccgcctggc agaacaaccc
    64801 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
    64861 ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg
    64921 taacggtctg cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc
    64981 tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc
    65041 aggcgaactg atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga
    65101 tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact
    65161 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
    65221 gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg
    65281 cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc
    65341 cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt
    65401 gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat
    65461 cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa
    65521 atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta
    65581 ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg aagcgacagt
    65641 caaagagcta tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct
    65701 ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc
    65761 gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac
    65821 cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat
    65881 ttatccgtac cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa
    65941 agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat
    66001 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
    66061 ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggcgcggtca gccgcattgg
    66121 tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct
    66181 ggcgcgcaag ccgattagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca
    66241 cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag
    66301 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct
    66361 gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga
    66421 actgtttaaa cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca
    66481 cgaggcgctg gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg
    66541 cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg
    66601 tcgcttcggc gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc
    66661 ccagggcgcg aagccgggcg aaggtggtca gttgccgggc gacaaagtca ctccttacat
    66721 cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga
    66781 tatctactct atcgaagact tagcgcagct tattttcgac ctcaagcagg ttaacccgaa
    66841 agcgatgatc tccgttaagc tggtgtccga accaggcgtt ggcaccatcg cgactggcgt
    66901 ggcgaaagct tatgcggact tgatcaccat cgcgggctat gacggcggca ccggcgcaag
    66961 cccgctgtcg tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca
    67021 acaggcgctg gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg atggcggcct
    67081 gaaaacgggt gtcgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg
    67141 cactggccca atggtggcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg
    67201 cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
    67261 gttcaaggtg actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
    67321 gctgggagta acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
    67381 cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga ctgccgagcc
    67441 acatgcaggt aagtcactct actgcactga aaacaacccg ccgtttgata acggcctact
    67501 gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt
    67561 ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc
    67621 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
    67681 cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga
    67741 tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt
    67801 tggttccgcc ttccgcagcc atgaagcaag cattatcggc aacacctgcc tgtatggcgc
    67861 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc
    67921 cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg
    67981 tattgtctgt attctgggta aaaccggcgt taacttcggc gcgggcatga ccggcggctt
    68041 cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactggtcga
    68101 agtcttaagc gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga
    68161 gcatgttcag cataccggtt ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt
    68221 cgccacgaaa ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca
    68281 ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag
    68341 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa
    68401 gatccgtaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
    68461 gcaggctgac cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca
    68521 caactacatc ccaaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcaga
    68581 actgtcgcat cagaccaaca cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg
    68641 tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat
    68701 tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt
    68761 gaagcaaacc ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc
    68821 ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg
    68881 cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg
    68941 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
    69001 cgtacagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg ttgggactta
    69061 tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc
    69121 gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt
    69181 cgtcagcatg gaaggcaaac gcgtggtagt acttggcggt ggcgacactg cgatggactg
    69241 cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga
    69301 agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt
    69361 caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt
    69421 aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat
    69481 cgttgcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg
    69541 tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat
    69601 catcgctccg gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg
    69661 cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc
    69721 ggcagacggc attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag
    69781 aaaagagtcg gcgctgaaat tctcctttct ttctggaaat cgcctcgcat aatctgaaga
    69841 aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt
    69901 ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac
    69961 ctcaacaggc taccagcaaa tccacaacgg cattattcat ctggtcgata gcgcccgaac
    70021 ggaaacggta cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg
    70081 gattgtcgaa tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga
    70141 tcggctatcg aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg
    70201 ccaaatgagg cttttttacc tctttt
//
LOCUS       DAJTYL010000005        65681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYL010000005 DAJTYL010000000
VERSION     DAJTYL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.005"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..2278)
                     /gene="glgP"
                     /locus_tag="OO572_000321"
     CDS             complement(<1..2278)
                     /gene="glgP"
                     /locus_tag="OO572_000321"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6659448.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLAD"
     gene            complement(2297..3730)
                     /gene="glgA"
                     /locus_tag="OO572_000322"
     CDS             complement(2297..3730)
                     /gene="glgA"
                     /locus_tag="OO572_000322"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6659449.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3730..5025)
                     /gene="glgC"
                     /locus_tag="OO572_000323"
     CDS             complement(3730..5025)
                     /gene="glgC"
                     /locus_tag="OO572_000323"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6659450.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5043..7016)
                     /gene="glgX"
                     /locus_tag="OO572_000324"
     CDS             complement(5043..7016)
                     /gene="glgX"
                     /locus_tag="OO572_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6659451.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7013..9199)
                     /gene="glgB"
                     /locus_tag="OO572_000325"
     CDS             complement(7013..9199)
                     /gene="glgB"
                     /locus_tag="OO572_000325"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6659452.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9472..10575)
                     /gene="asd"
                     /locus_tag="OO572_000326"
     CDS             complement(9472..10575)
                     /gene="asd"
                     /locus_tag="OO572_000326"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6659453.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10767..11360
                     /gene="yhgN"
                     /locus_tag="OO572_000327"
     CDS             10767..11360
                     /gene="yhgN"
                     /locus_tag="OO572_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6659454.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11417..12757)
                     /gene="gntU"
                     /locus_tag="OO572_000328"
     CDS             complement(11417..12757)
                     /gene="gntU"
                     /locus_tag="OO572_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6659455.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12761..13288)
                     /gene="gntK"
                     /locus_tag="OO572_000329"
     CDS             complement(12761..13288)
                     /gene="gntK"
                     /locus_tag="OO572_000329"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6659456.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13427..14422)
                     /gene="gntR"
                     /locus_tag="OO572_000330"
     CDS             complement(13427..14422)
                     /gene="gntR"
                     /locus_tag="OO572_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6659457.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14646..15341)
                     /gene="yhhW"
                     /locus_tag="OO572_000331"
     CDS             complement(14646..15341)
                     /gene="yhhW"
                     /locus_tag="OO572_000331"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6659458.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15464..16501)
                     /locus_tag="OO572_000332"
     CDS             complement(15464..16501)
                     /locus_tag="OO572_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6659459.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16834..17322
                     /gene="yhhY"
                     /locus_tag="OO572_000333"
     CDS             16834..17322
                     /gene="yhhY"
                     /locus_tag="OO572_000333"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6659460.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17381..19123)
                     /gene="ggt"
                     /locus_tag="OO572_000334"
     CDS             complement(17381..19123)
                     /gene="ggt"
                     /locus_tag="OO572_000334"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6659461.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19243..19683
                     /locus_tag="OO572_000335"
     CDS             19243..19683
                     /locus_tag="OO572_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6659462.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19670..20413)
                     /gene="ugpQ"
                     /locus_tag="OO572_000336"
     CDS             complement(19670..20413)
                     /gene="ugpQ"
                     /locus_tag="OO572_000336"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6659463.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20410..21400)
                     /locus_tag="OO572_000337"
                     /pseudo
     CDS             complement(20410..21400)
                     /locus_tag="OO572_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21402..22247)
                     /gene="ugpE"
                     /locus_tag="OO572_000338"
     CDS             complement(21402..22247)
                     /gene="ugpE"
                     /locus_tag="OO572_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6659464.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22244..23131)
                     /gene="ugpA"
                     /locus_tag="OO572_000339"
     CDS             complement(22244..23131)
                     /gene="ugpA"
                     /locus_tag="OO572_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6659465.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23229..24545)
                     /gene="ugpB"
                     /locus_tag="OO572_000340"
     CDS             complement(23229..24545)
                     /gene="ugpB"
                     /locus_tag="OO572_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6659466.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(24942..25655)
                     /gene="livF"
                     /locus_tag="OO572_000341"
     CDS             complement(24942..25655)
                     /gene="livF"
                     /locus_tag="OO572_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6659467.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25657..26424)
                     /gene="livG"
                     /locus_tag="OO572_000342"
     CDS             complement(25657..26424)
                     /gene="livG"
                     /locus_tag="OO572_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6659468.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26421..27698)
                     /gene="livM"
                     /locus_tag="OO572_000343"
     CDS             complement(26421..27698)
                     /gene="livM"
                     /locus_tag="OO572_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6659469.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27695..28621)
                     /gene="livH"
                     /locus_tag="OO572_000344"
     CDS             complement(27695..28621)
                     /gene="livH"
                     /locus_tag="OO572_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6659470.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28669..29778)
                     /gene="livK"
                     /locus_tag="OO572_000345"
     CDS             complement(28669..29778)
                     /gene="livK"
                     /locus_tag="OO572_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6659471.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30202..30585
                     /gene="panM"
                     /locus_tag="OO572_000346"
     CDS             30202..30585
                     /gene="panM"
                     /locus_tag="OO572_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6659472.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30784..31887)
                     /gene="livJ"
                     /locus_tag="OO572_000347"
     CDS             complement(30784..31887)
                     /gene="livJ"
                     /locus_tag="OO572_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6659473.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32158..33012)
                     /gene="rpoH"
                     /locus_tag="OO572_000348"
     CDS             complement(32158..33012)
                     /gene="rpoH"
                     /locus_tag="OO572_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6659474.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33257..34315)
                     /gene="ftsX"
                     /locus_tag="OO572_000349"
     CDS             complement(33257..34315)
                     /gene="ftsX"
                     /locus_tag="OO572_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6659475.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34308..34976)
                     /gene="ftsE"
                     /locus_tag="OO572_000350"
     CDS             complement(34308..34976)
                     /gene="ftsE"
                     /locus_tag="OO572_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6659476.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(34979..36475)
                     /gene="ftsY"
                     /locus_tag="OO572_000351"
     CDS             complement(34979..36475)
                     /gene="ftsY"
                     /locus_tag="OO572_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6659477.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36625..37221
                     /gene="rsmD"
                     /locus_tag="OO572_000352"
     CDS             36625..37221
                     /gene="rsmD"
                     /locus_tag="OO572_000352"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6659478.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37211..37480
                     /locus_tag="OO572_000353"
     CDS             37211..37480
                     /locus_tag="OO572_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6659479.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37483..37842)
                     /locus_tag="OO572_000354"
     CDS             complement(37483..37842)
                     /locus_tag="OO572_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6659480.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            37983..38609
                     /locus_tag="OO572_000355"
     CDS             37983..38609
                     /locus_tag="OO572_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6659481.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38683..40881
                     /gene="zntA"
                     /locus_tag="OO572_000356"
     CDS             38683..40881
                     /gene="zntA"
                     /locus_tag="OO572_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6659482.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40983..41228)
                     /gene="tusA"
                     /locus_tag="OO572_000357"
     CDS             complement(40983..41228)
                     /gene="tusA"
                     /locus_tag="OO572_000357"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6659483.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41449..42114
                     /locus_tag="OO572_000358"
     CDS             41449..42114
                     /locus_tag="OO572_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6659484.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42187..42744
                     /gene="dcrB"
                     /locus_tag="OO572_000359"
     CDS             42187..42744
                     /gene="dcrB"
                     /locus_tag="OO572_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6659485.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42748..43965)
                     /locus_tag="OO572_000360"
     CDS             complement(42748..43965)
                     /locus_tag="OO572_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6659486.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44099..45148
                     /locus_tag="OO572_000361"
     CDS             44099..45148
                     /locus_tag="OO572_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6659487.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45203..45790
                     /gene="acpT"
                     /locus_tag="OO572_000362"
     CDS             45203..45790
                     /gene="acpT"
                     /locus_tag="OO572_000362"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6659488.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45901..47475
                     /gene="nikA"
                     /locus_tag="OO572_000363"
     CDS             45901..47475
                     /gene="nikA"
                     /locus_tag="OO572_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6659489.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47475..48419
                     /gene="nikB"
                     /locus_tag="OO572_000364"
     CDS             47475..48419
                     /gene="nikB"
                     /locus_tag="OO572_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6659490.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48416..49249
                     /gene="nikC"
                     /locus_tag="OO572_000365"
     CDS             48416..49249
                     /gene="nikC"
                     /locus_tag="OO572_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6659491.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49249..50013
                     /gene="nikD"
                     /locus_tag="OO572_000366"
     CDS             49249..50013
                     /gene="nikD"
                     /locus_tag="OO572_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6659492.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50010..50816
                     /gene="nikE"
                     /locus_tag="OO572_000367"
     CDS             50010..50816
                     /gene="nikE"
                     /locus_tag="OO572_000367"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6659493.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50822..51223
                     /gene="nikR"
                     /locus_tag="OO572_000368"
     CDS             50822..51223
                     /gene="nikR"
                     /locus_tag="OO572_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6659494.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51343..51702)
                     /locus_tag="OO572_000369"
     CDS             complement(51343..51702)
                     /locus_tag="OO572_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6659495.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51699..51974)
                     /locus_tag="OO572_000370"
     CDS             complement(51699..51974)
                     /locus_tag="OO572_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6659496.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52033..53157)
                     /locus_tag="OO572_000371"
     CDS             complement(52033..53157)
                     /locus_tag="OO572_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6659497.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53157..55892)
                     /gene="rbbA"
                     /locus_tag="OO572_000372"
     CDS             complement(53157..55892)
                     /gene="rbbA"
                     /locus_tag="OO572_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6659498.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55889..56956)
                     /locus_tag="OO572_000373"
     CDS             complement(55889..56956)
                     /locus_tag="OO572_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6659499.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57322..58944)
                     /locus_tag="OO572_000374"
     CDS             complement(57322..58944)
                     /locus_tag="OO572_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6659500.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59206..60795)
                     /locus_tag="OO572_000375"
     CDS             complement(59206..60795)
                     /locus_tag="OO572_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6659501.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61180..62211
                     /locus_tag="OO572_000376"
                     /pseudo
     CDS             61180..62211
                     /locus_tag="OO572_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62298..62774)
                     /locus_tag="OO572_000377"
     CDS             complement(62298..62774)
                     /locus_tag="OO572_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659502.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62767..63531)
                     /locus_tag="OO572_000378"
     CDS             complement(62767..63531)
                     /locus_tag="OO572_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6659503.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63777..64538
                     /locus_tag="OO572_000379"
                     /pseudo
     CDS             63777..64538
                     /locus_tag="OO572_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64645..65145)
                     /locus_tag="OO572_000380"
     CDS             complement(64645..65145)
                     /locus_tag="OO572_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659504.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65296..>65681)
                     /locus_tag="OO572_000381"
     CDS             complement(65296..>65681)
                     /locus_tag="OO572_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6659505.1"
                     /translation="RDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKG
                     LVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQ
                     SCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        15959 a        17646 c        16155 g        15921 t
ORIGIN      
        1 aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc agatcgcgat
       61 agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc acctgatgca
      121 gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc agttcttcca
      181 cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga tccagcatct
      241 cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca aacttcatgt
      301 tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga tctgccgccg
      361 gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact ttcagcttat
      421 cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa tgaataatgt
      481 gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg cgcggtaccc
      541 acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc aacacattca
      601 tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac gcctttggat
      661 tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg ttctccagct
      721 tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc agctcattaa
      781 gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg gctgaaagcg
      841 atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac
      901 gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga ttagagtgca
      961 gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc atacgcacac
     1021 gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta tcgttcggat
     1081 actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc tgcaggtgac
     1141 gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg
     1201 tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt
     1261 ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga tgggtatcat
     1321 tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac tgataatgac
     1381 ggcttaaaat gtcctgaatg gtcgaggaaa ccaagaagta ttcctgacgc aggcgcagct
     1441 cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc tcggagtggt
     1501 ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg ttaatttcgc
     1561 tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg taaccaggga
     1621 ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc gtttttttac
     1681 cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt
     1741 cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta ccgttaacga
     1801 tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc ggcaacccta
     1861 acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg ttaccgaggc
     1921 ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct tccagtgcgc
     1981 cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta cggccaatca
     2041 aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg gcacggtttg
     2101 aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg ttcagccatt
     2161 catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg taagcgatag
     2221 agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat
     2281 aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac ttcgccgcga
     2341 cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac agtgaaggac
     2401 gggaccacag tacaaaagca cgccgaatag cccgtaacag cgaccaggca ttactatctt
     2461 caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag tcagaaaccg
     2521 tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc aatccataaa
     2581 gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc gcgccgccca
     2641 taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctga ccggggtatt
     2701 ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg agtagcgcca
     2761 gctgcccgcc ctgctccaga agccccggta aggcttccag caccaggtcg agacctttct
     2821 ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca
     2881 ttgcgatttg taactggctc ttattttccg ctttatcttc caacgtatcg cgggtgtaac
     2941 gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg ccgttcagta
     3001 cgcctgaaag acgcccttca cggtgacgct gttgcaacag accttccata ccgtaggcaa
     3061 actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg tgatcggcat
     3121 agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca tgaatattaa
     3181 agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg ccttgatagg
     3241 ccaggttgtg cacagtaaac accgacttcg caggacgccc gcgcgccgcc agatacgcag
     3301 gcgcaagacc tgcatgccag tcgtgcacat gcaccacatc aggacgccag aatgggtcaa
     3361 gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg
     3421 tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag agatgcggcg
     3481 cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg atatgcccgg
     3541 cgaaagtatc acgacgggat actacctgcg catcggtcac gccacggcga atatcgggaa
     3601 atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc
     3661 caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa catacatgta
     3721 aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc gcgttaccag
     3781 cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt tttcaccaat
     3841 caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca gacggcacga
     3901 gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga atgaattcac
     3961 gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca cacaaccgcc
     4021 ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca cgaatttcgc
     4081 tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca tatccagttc
     4141 cggcaccaca gaggccagat cgagattcgc tttccagtaa gcttccagcg tacccacatc
     4201 gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga acgggtgcgc
     4261 ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt cgtggctgga
     4321 gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt caaagacgta
     4381 gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg ggttagcagg
     4441 tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc caaatgcgga
     4501 ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg
     4561 atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca ggatcaccac
     4621 gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat ctgcggtgcc
     4681 gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga caaactcgtt
     4741 catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag tgtgggactg
     4801 gtactgggtg atcacgccca tacgacggat cccagagttg atgcagttag acagcgcaaa
     4861 gtcaataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct tattggttaa
     4921 atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt tcaatggcag
     4981 ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta actccttttt
     5041 tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacaac cgtaatcact
     5101 gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg taaaacaatc
     5161 tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag cagaatttgc
     5221 agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata tcgatttagc
     5281 caacggacat tgccgtcacc ttcttcccac cagcgattct ccaccaaagc gggaatgcgc
     5341 ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag
     5401 tccaaccagt ttaattggtt atcctgacag taggcattgt tattgccatg ctggctgtga
     5461 ccatgttcgt caccggccag taacatcggc gtgccctggg agagcaacaa cgttgttaac
     5521 agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa gagtaccgcc taacccttct
     5581 ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc tccgtttgct
     5641 tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc atgcgcggtg
     5701 acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc gctggaggca
     5761 gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa acgacgggca
     5821 gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc cacctgataa
     5881 ccactaggag cgatatccca cggttcagca attaacttca cctgcgagag caccgggcag
     5941 ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg gcccatgact
     6001 gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata acgcaggcag
     6061 gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc gcaaccggtc
     6121 cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc gatcccacgc
     6181 agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac gatatcaaga
     6241 atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc atccagcgcc
     6301 gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc
     6361 cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg cgccactggc
     6421 agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac cggatgcccg
     6481 agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta cgttaatcct
     6541 ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg ggcgtcatct
     6601 tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc gttgtcgcga
     6661 tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc cccgtcaatt
     6721 tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc ctcggcgggt
     6781 tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc cggcagataa
     6841 ccgtgccaaa tgtcgccact gttccctggc aagtcatagc gatgttcctg gccattggcg
     6901 tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc
     6961 tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg tgtcattctg
     7021 cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt gaatgctgac
     7081 gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca ttactgccgt
     7141 gatagtgtat ggaatcggta ttgaggattt cacgccattt acctggctga ttaatgccga
     7201 agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt tcgttaccct
     7261 ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc accagccatt
     7321 caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg taggtgaggt
     7381 tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct tccaacagat
     7441 gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg ttccccatga
     7501 acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc aggttagcga
     7561 atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg tggaccactt
     7621 catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca
     7681 gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc agggtgtcat
     7741 gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc tgcggacgag
     7801 aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa acctgctcac
     7861 caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg cgcccgccaa
     7921 attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc atcgacgcca
     7981 ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc gcgttaccga
     8041 cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc cagtcctgat
     8101 gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat tcggcaagcg
     8161 caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc agaccagctg
     8221 cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg cgggttggtg
     8281 catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg ttaatgggca
     8341 gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga tcggccagct
     8401 cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc aggtgaactt
     8461 cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca gtctgtacaa
     8521 ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc gcttcgaagg
     8581 cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg tatttataga
     8641 gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct ttacgcaggc
     8701 gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag acccgacggg
     8761 cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg
     8821 cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag gcatccattt
     8881 cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga ccatgccaga
     8941 caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg ctaaagaatc
     9001 cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt tcaatcaccc
     9061 acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg gttttatgca
     9121 ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc
     9181 tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca tccaattgga
     9241 tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc tgtgcatcct
     9301 ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag ccacccttaa
     9361 agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg cttactcctg
     9421 tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag attacgccag
     9481 ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt cgcccacggt
     9541 aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta ccggcgtggt
     9601 cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc gatcgttcgg
     9661 aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg gaatagacac
     9721 atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac
     9781 acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg tttccgcctg
     9841 ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag
     9901 gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac gggttaaggt
     9961 tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt catctgccac
    10021 atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg caccaccgcc
    10081 ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg cgaataaacc
    10141 acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag tcctgatgcc
    10201 attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga tgatggcgtc
    10261 atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc cgctttcacg
    10321 aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa tgatatcgag
    10381 ggcctttagc gcctctagat caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga
    10441 cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc
    10501 gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc agccgataaa
    10561 accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa atgtgccggt
    10621 ctcctcttgg cacatctttc accatacaaa aagctgccaa agtcgcaagt gaaattaatc
    10681 aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg cagaaagtcc
    10741 gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca gttttattga
    10801 tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg aaacatactg
    10861 aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc ctggtgatgc
    10921 tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga gcagaaaccg
    10981 tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt ttccccagcg
    11041 cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg ccgttggcaa
    11101 ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct catcagtacc
    11161 cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc acctttgtca
    11221 tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg aacgcacttg
    11281 aacgcctgat gggattgatt ctggtgatga tggcaactca gatgttcctc gacggcattc
    11341 gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gagcccggta gcgtcacaac
    11401 taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg atggcaccaa
    11461 cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct tcggtcgcgc
    11521 cggtaaattt accgaacaac cagaaaccag cgtcgttaac gtggctgaca acaatcgaac
    11581 caccagcgat acaaatcgac agtgccgcca tttgcgcacc agagtagttc agttgttcaa
    11641 tgactggcat caccagtccc accgccgtta aacaggctac ggtggcagcc ccctgaatga
    11701 tgcgcactgc agcagccagc acgaagcagg tgatagcaat cggcaggccc atgccggtta
    11761 acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg aacacgccgc
    11821 ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg caaatctcca
    11881 tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct accagacaag
    11941 caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa gcggtagagc
    12001 cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc ggcaacagga
    12061 tcagcgacag gctgaatccg aaagatggca ttttaccttc gccgagatgc ggttcgctga
    12121 tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa gttaccccac agcggcccgg
    12181 caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag ccaaagtcgg
    12241 cattcatctg cgatgccagc agcattggcg ctggcccagg caccaggaac gctgccgcgg
    12301 ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg gtgtggcgcg
    12361 ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac agcggtagcg
    12421 cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg aaggatttga
    12481 gcattttgac ggcaatctga tcgactgcgc cggtttcata taagatcttg ccaaacatag
    12541 ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct ttttccatcg
    12601 tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg gacaccacca
    12661 tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc agtaaaacag
    12721 accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct tttttaataa
    12781 cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc accagtacat
    12841 cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac atttgggttt
    12901 taaagaaatg gcctttgcgc gctttcaggc gactttcaat cacatcaaaa tcgcctttca
    12961 ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag tgctttttca
    13021 gtgcagaaca ggcgatcagc gacactttat tggtgcgctg catagcaaac gcggagtcgt
    13081 tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca gacgccattt
    13141 tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca tgaagttgat
    13201 gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc aagacgtaaa
    13261 tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag aattaaaaat
    13321 gttacgggta actgttatcg gcaacattgt ccagccggac aatatcagaa gcaatatcca
    13381 tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa atagatccgc
    13441 ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg ccacgaatac
    13501 gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc agcacgctcg
    13561 caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca atcgccatat
    13621 cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc gccagatcat
    13681 cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg cgaatcagtt
    13741 caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc accaggcctg
    13801 catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg tcgagacgtg
    13861 cgccgagata ggcaatgtgg cgatgcccac gagcaataat ggcggtagtc atctggcgtg
    13921 ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg ctgtccatca
    13981 actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc gtgtgggtac
    14041 gttcggtgag gatcaggccg tcgatattcc aggagagcat ggattcgaga cgttcttgct
    14101 ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc gcgtcggtga
    14161 cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa ggtaacagga
    14221 cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg ggaatatagc
    14281 ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc tgctccgggt
    14341 tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc acatcctgaa
    14401 gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt ctcttagttt
    14461 aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat tattagatat
    14521 acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg aagcgtgcat
    14581 cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa agatgccgtt
    14641 gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc cgcatggatg
    14701 gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt cacgccgtta
    14761 atggtaacat tgccttttac cacctgaatc cagacgcggc gttcagcggc aatctgatgc
    14821 accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg atgcactttc
    14881 agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac ggcatcgaag
    14941 cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca gatctgatac
    15001 agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc cgtacccgca
    15061 ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct gtcctgatgc
    15121 tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg gtgagtgccg
    15181 aagccctgcc ctacttcaat cacgtcgtcg ttaatcacgc gcagcgcgga gaagcccata
    15241 aagttcggat cgtaatagtt ggcacaagag aaagtatgcc aggagtccag ccagccatga
    15301 tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg ccccctgaat
    15361 gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt gaaaattgcc
    15421 ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc gcgagagtca
    15481 ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg gtaagaactt
    15541 cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac gcatcataaa
    15601 cacgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcaca ccctcgtcat
    15661 tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg cccggcataa
    15721 tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac gaacctttct
    15781 taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt ttgacgatgg
    15841 ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg gctttattac
    15901 gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa ataatctggt
    15961 ccatcgtatg cacaccaagg ccatagaacg cgccatcctg cggcagtcca ggtttggttt
    16021 ctgccaccgg gcggtaatag tcaaaatggc tttccacttc gacaatctct cccagcttgc
    16081 cgctttcaat cgcttttttc gccgtcagga agcaggaatc aaagcgacga ttctgatatg
    16141 gcgtgacggt cagccctttg cttttcgcca gtgcaaacag ctctttcgcc tgcgcaagtg
    16201 tcggagtgaa cgttttttcg accagcacat ttttcccggc ttccagcgcg cgcttcgcgt
    16261 actcgaagtg gctgtccgca tgggtgcaga caacaaccaa cttaacatcg ggatcgttta
    16321 gcacttcgtc gagatcgctg gtgaaatgga tatgagagta aatgggagcc tgttcttccg
    16381 gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg ttaagtacat
    16441 acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag ttgatgacca
    16501 tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac gtggttccta
    16561 ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg aaagatcaaa
    16621 aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca atgtcgtgct
    16681 ttcaggttct ccgcgagggt cttcctgatc gcgatacaat aataatcatt ctcattcgca
    16741 cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt caatgatgtt
    16801 gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat acgccacgca
    16861 gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt gtattgcaac
    16921 acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc cgatcgtccc
    16981 ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct caccattgac
    17041 gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt cgactctcgt
    17101 tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat gtgcgacaac
    17161 tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc agcaattaag
    17221 gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc attgcgtaat
    17281 ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg caatctaagt
    17341 ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc gccgttaaat
    17401 catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca accataatgc
    17461 tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct tccagcagct
    17521 tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac cactgatggt
    17581 ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg ctattcacca
    17641 ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta accagccagg
    17701 ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt ttgttcggcc
    17761 cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc ggtttggcgg
    17821 agaaatcatc catctggtta ttaagcagaa taccgctctc acccgcgaca atacccgtac
    17881 cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta tccactacag
    17941 agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga atctcactgg
    18001 acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag gctttattgg
    18061 tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc gagcggtcgg
    18121 cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg ccaaagccgt
    18181 atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg atcccgccgg
    18241 aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt atcggagtgc
    18301 gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg tttttctgca
    18361 tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg ccgttttcag
    18421 caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgtcgccc tttttcagcg
    18481 gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac acttcgctac
    18541 cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg cgtgccagtt
    18601 taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc agcgccagcg
    18661 agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt gattttttgc
    18721 tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg ggtgccattt
    18781 cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata aaaccaccac
    18841 cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc gccacggcgg
    18901 catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg gcagtggcgt
    18961 ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct tcctccacac
    19021 catacgagac gggcggcgca ggaggcgcgg cggcggcgct aaaacaactt cctgagagca
    19081 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19141 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19201 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19261 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19321 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19381 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19441 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19501 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19561 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacagc
    19621 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19681 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19741 gcaactctgc cgcgtgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19801 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    19861 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    19921 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    19981 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20041 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20101 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20161 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20221 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20281 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20341 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20401 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20461 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20521 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20581 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20641 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20701 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20761 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    20821 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    20881 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    20941 ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21001 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21061 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21121 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21181 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21241 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21301 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21361 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21421 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21481 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21541 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21601 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21661 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21721 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21781 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    21841 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    21901 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    21961 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22021 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22081 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22141 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22201 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22261 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcagcacca
    22321 tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22381 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22441 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22501 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22561 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22621 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22681 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22741 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22801 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    22861 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    22921 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    22981 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23041 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23101 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23161 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23221 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23281 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23341 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23401 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23461 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23521 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23581 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23641 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23701 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23761 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    23821 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    23881 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    23941 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24001 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24061 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacgga agtcgagctg
    24121 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24181 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24241 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24301 ggagcgttgc cggtacgaaa tacggcaatc cccgcgctta aattctgttc gtagttgcct
    24361 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24421 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24481 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24541 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24601 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24661 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24721 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24781 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    24841 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    24901 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    24961 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25021 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25081 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25141 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25201 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25261 acacggcgct catgcaaacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25321 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25381 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25441 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25501 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25561 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25621 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25681 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25741 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25801 caaaatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    25861 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    25921 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    25981 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26041 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26101 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26161 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26221 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26281 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26341 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26401 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26461 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26521 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26581 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26641 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26701 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcagtc
    26761 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    26821 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    26881 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    26941 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27001 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27061 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27121 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27181 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27241 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27301 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27361 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27421 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27481 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27541 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27601 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27661 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27721 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27781 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    27841 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    27901 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    27961 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28021 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28081 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28141 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28201 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28261 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28321 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28381 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28441 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28501 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28561 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28621 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28681 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28741 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28801 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    28861 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    28921 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    28981 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29041 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29101 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29161 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29221 tttcagcccg tcctgcaccg aacgcgccag cccttcgcca tactgttgtt tgtcgtgaat
    29281 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29341 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29401 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29461 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29521 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29581 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29641 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29701 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29761 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    29821 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    29881 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    29941 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30001 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30061 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30121 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30181 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30241 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30301 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30361 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30421 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30481 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30541 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30601 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30661 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30721 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30781 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    30841 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    30901 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    30961 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31021 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31081 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31141 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31201 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31261 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31321 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31381 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31441 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31501 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31561 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31621 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31681 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31741 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31801 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    31861 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    31921 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    31981 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32041 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32101 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32161 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32221 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32281 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32341 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32401 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32461 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32521 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32581 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32641 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32701 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32761 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    32821 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    32881 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    32941 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33001 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33061 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33121 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33181 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33241 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33301 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33361 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33421 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33481 gcagcgcacc gccgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33541 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33601 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33661 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaataccgt
    33721 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33781 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    33841 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    33901 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    33961 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34021 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34081 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34141 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34201 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34261 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34321 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34381 agattaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34441 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34501 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34561 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34621 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34681 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34741 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34801 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    34861 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    34921 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    34981 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35041 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35101 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35161 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35221 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35281 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35341 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35401 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35461 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35521 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35581 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35641 tctcgcccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35701 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35761 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    35821 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    35881 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct gcaaccggct
    35941 gctcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36001 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36061 cgacaatctc tacggtttcc gcttcagcct gccactcttc tgacgaaacc tcttcggcgt
    36121 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36181 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36241 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36301 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36361 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36421 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36481 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36541 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36601 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36661 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36721 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36781 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    36841 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    36901 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    36961 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37021 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37081 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37141 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37201 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37261 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37321 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37381 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37441 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37501 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccactg tcagggcgtg
    37561 gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37621 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37681 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37741 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37801 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    37861 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgccact
    37921 aaactggaag caagaccgta ggcattctgc ttacgaaaaa ataacgaatt caaggaacta
    37981 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38041 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38101 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38161 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38221 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38281 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38341 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38401 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38461 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38521 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38581 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38641 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38701 cacggcaaga aagcccctca atttgctgcg ttcaaaccgc taaccacggt acagaacgcc
    38761 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    38821 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    38881 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    38941 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39001 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39061 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39121 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39181 attgctcgtc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39241 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39301 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39361 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39421 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39481 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39541 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39601 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39661 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39721 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39781 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    39841 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    39901 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    39961 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40021 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40081 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40141 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40201 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40261 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40321 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40381 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40441 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40501 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40561 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40621 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40681 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40741 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40801 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    40861 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    40921 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    40981 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41041 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41101 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41161 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41221 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41281 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41341 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41401 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41461 tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc
    41521 acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg
    41581 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41641 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41701 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41761 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    41821 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    41881 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    41941 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42001 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42061 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42121 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42181 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42241 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggttgcgga aagtaatgct
    42301 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42361 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42421 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42481 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42541 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42601 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42661 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42721 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42781 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    42841 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    42901 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctgattttg
    42961 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43021 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43081 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43141 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43201 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43261 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43321 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43381 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43441 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43501 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43561 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43621 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43681 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43741 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43801 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    43861 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    43921 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    43981 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44041 atattgctgg cgtgactccc gcgctacact taacacaaaa agtcacaagg aagccccaat
    44101 ggaaacccct caacccgata aaacgggcat gcacattctg ctcaagttgg cctcgctggt
    44161 agtgatcctc gcgggcattc acgcagcggc agatatcatt gtgcagctgt tactggcgct
    44221 gttttttgcc atcgtcctca acccgctcgt tacctggttt attcgtcggg gagtacaacg
    44281 ccccgttgcc attacgattg tagtggtggt gatgctgatc gcactaaccg cgctggtcgg
    44341 cgtactggcg gcatcgttta acgaatttat ctctatgctg ccgaagttta ataaggagct
    44401 gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt cttaatttgc atatgtcgcc
    44461 ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt accttcacca cagcgctaat
    44521 gaccgggctt tccggggcaa tggcgagcgt gcttttgctg gtgatgaccg tagtttttat
    44581 gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt gcgctgaata acccacagat
    44641 tcacatcgca ggattacacc gcgcacttaa aggcgtttcg cattatctgg cgttaaagac
    44701 gctactcagt ttatggacag gtgtcatcgt ctggctgggg ctggcgctaa tgggcgtaca
    44761 gtttgcgctg atgtgggcag tactggcgtt tttgctcaac tacgtgccca atatcggcgc
    44821 ggtaatttcc gccgtaccgc caatgattca ggtgctgctg tttaatggtg tttacgaatg
    44881 tattctggtc ggcgcattgt ttttagtggt ccatatggtc atcggcaata ttttagaacc
    44941 acggatgatg ggccatcgcc tggggatgtc caccatggtg gtatttcttt cattgttaat
    45001 ctggggatgg ctgctcggcc cggtagggat gctactttcg gtgccattaa ccagcgtgtg
    45061 taaaatctgg atggaaacga cgaaaggtgg cagcaaactg gcgattttac tggggccggg
    45121 cagaccgaaa agtcggttac cgggatgagg cgacaagtga tacgatacgc actttcattt
    45181 tccattaaac gttggccctg atatgtatcg gatagttctg gggaaagttt cgaccttaag
    45241 cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg caaggtccac gacgcgaacg
    45301 ctggctggcg gggcgtacat tgctttcgca cacgctttcc ccgctaccgg agatcatcta
    45361 tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg ctatggttca acttaagcca
    45421 tagcggtgac gatatcgctc tgctgttgag cgatgaaggc gaagtcggct gcgatatcga
    45481 agtgattcgc ccgcgcgtca actggcgctg gctggcgaac gccgtgttca gcctcgggga
    45541 acacgctgaa atggacgccg tgcatcctga tcagcaactg gaaatgttct ggcgcatctg
    45601 gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc gcctggcaaa tcgtcagcgt
    45661 agatagcacc tatcactcct cgctgtcagt cagccattgt cagctagaaa atttaagcct
    45721 tgcgatctgc acccccaccc cctttacgct caccgccgac agtgtgcaat ggatcgattc
    45781 agttaactga tccgcccacc cgactgccca tctattgatc cagaacaggt aatcagtatg
    45841 acgaatactt aaaatcgtca tacttatttc cgccatctat tttaatccat tggggttacc
    45901 atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc ttttatcgtc
    45961 catgccgctg caccagatga aatcaccacc gcctggccgg tgaatgtcgg gccactaaac
    46021 ccgcaccttt acacgcctaa ccagatgttc gcccagagca tggtttatga accattggtg
    46081 aaatatcagg cagacggttc ggtgatcccg tggctggcaa aaagctggac tcattcagaa
    46141 gatggtaaaa cctggacctt caccctgcgt gatgacgtga aattctccaa cggtgaaccg
    46201 ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc tcgataaccg tcaacgtcac
    46261 gcctggctgg agctggcaaa ccagattgtt gatgttaaag cactcaataa aacagagctg
    46321 caaattaccc tgaaaagcgc ctactatcct ttcctgcaag aactggccct gccccgtcct
    46381 ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa ccatgaacgg aattaaagcg
    46441 ccgattggca ccggaccgtg gattttgcag gaatcgaaac taaatcagta cgatgtcttc
    46501 gtccgtaacg aaaactactg gggcgaaaag ccagcgatta aaaagatcac ctttaacgtc
    46561 atcccggacc cgactacccg cgcggtggcg tttgaaactg gcgatatcga cctgctgtac
    46621 ggaaacgaag ggttattacc gctcgatacc ttcgcccgct ttagccagaa tccggcttac
    46681 cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg cgctcaatac cgccaaagcc
    46741 cccaccaacg agctggcagt acgtgaagct cttaattacg cggtaaacaa aaaatcgctg
    46801 attgataacg cgttatatgg cacccagcag gtcgccgaca ccctgtttgc cccttctgtg
    46861 ccttacgcta atctcggcct gaaaccgcgc cagtacgatc cgcagaaagc gaaagagttg
    46921 ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca tccgcgagaa aaatggtcag
    46981 ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt taagcaaatc gatggcggaa
    47041 atcattcagg ctgatatgcg ccagattggc gcagatgtct cgctgattgg cgaagaagag
    47101 agcagtatct atgctcgtca gcgcgacggt cgttttggca tgattttcca ccgcacctgg
    47161 ggcgcgccat acgatccaca cgccttcctg agttcaatgc gcgtaccgtc gcacgctgac
    47221 ttccaggcac agcaaggatt agccgacaag ccgctgattg ataaagagat cggcgaagtg
    47281 ctggcgaccc atgacgaaac gcaacgtcag gcgctgtatc gcgacattct gacccgtctg
    47341 catgacgagg cggtttatct gcctatcagc tacatctcga tgatggtggt atcaaaaccg
    47401 gagctgggta atatccccta cgcgccgatc gccaccgaaa ttccgttcga acagattaaa
    47461 ccggtgaaac cttaatgttg cgttacgtat tacgccgctt tctgctgctg atcccgatgg
    47521 tgctcgccgc ctcggtgatc atttttctga tgctgcgcct cggtaccggc gacccggcgc
    47581 tcgattatct gcgtttatct aacctgccgc cgacgccgga gatgctggcc tctacccgca
    47641 ccatgctcgg gctggatcag ccgctgtacg tccagtacgg tacctggttg tggaaggcgc
    47701 tgcatcttga ctttggtatc tcattcgcca gccaacgccc ggtactggac gatatgctga
    47761 acttcctgcc cgccacgctg gaacttgcag gtgcggcgct ggtattaatt ctgctcactt
    47821 ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg tttgccggat ttcgccgtgc
    47881 gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg gctggcattt ttgctagtga
    47941 tggcgttttc ggtgtatctg caatggctac ccgcgatggg ctacggcagc tggcagcaca
    48001 tcattttacc tgcggtttcc atcgccttta tgtcgctggc gattaacgcg cgactgctgc
    48061 gcgccagtat gctggacgtc gccggtcagc gtcacgtcac ctgggcgcgg ctacgcggcc
    48121 tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa tgcctcgctg ccgatgatca
    48181 ccgccgtggg gatgcacatt ggcgaattga ttggcgggac gatgattatc gaaaacatct
    48241 ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat ttttaaccgt gactatccgg
    48301 tgattcagtg ctttacgctg atgatggtgg tggtttttgt ggtctgtaat ttgattgtcg
    48361 atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca tgaaggagcg cacgcgtgaa
    48421 ctttttcctc tcttcccgct ggtcggtacg cctggcgctg atcattatcg ccctgctggc
    48481 gctgattgcg ctcaccagcc agtggtggca gccgtacgat ccacaggcaa ttgatttgcc
    48541 gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc accgatcact taggtcgcga
    48601 tattttctcg cggctgatgg cagcgacccg cgtgtcgctc ggttcggtaa tggcctgcct
    48661 gctgctggtg ctgacattag ggctggttat tggcggcagc gccgggttga ttggcgggca
    48721 cgttgatcag gccaccatgc gcgtcgccga tatgtttatg accttcccga cctcgattct
    48781 gtcgttcttt atggttggcg tgctcggcac cgggctgacc aacgtaatta tcgccatcgc
    48841 cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc ttggtgattt cactacgcca
    48901 acgcgagttt gtgctggcgt cacggctttc cggtgcgggc catgtgcggg tgtttgtcga
    48961 tcatctggca ggcgcggtga tcccttcgct gctggtgctg gcaacgctgg atatcggcca
    49021 tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc ggtgtgaccg cgccgaccgc
    49081 cgaatggggc gtgatgatta acgacgcgcg ccagtatatc tggacccagc cgctgcaaat
    49141 gttctggccg gggctggcgc tgtttatcag cgtgatggcc tttaacctgg tgggtgacgc
    49201 actgcgcgat catctggatc ctcatctggt gacggagcac gcacactaat gccgcaacag
    49261 attgaactac gtaatatcac gctacaggcc gcgcagccgc tggtacacgg tgtatcgtta
    49321 accctgcaac gcgggcgcgt gctggcgtta gtcggcggta gcggcagcgg gaaatcatta
    49381 acctgcgccg cgacgctggg cattttgccc gctggcgttc gccagacggc gggggaaatt
    49441 ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg gcatcaaaat tgccaccatc
    49501 atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca tgcacaccca cgcgcgggaa
    49561 acctgcctgg cgttagggaa acccgccgat gacgctacgc ttaccgctgc catagaagcg
    49621 gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc cgttcgagat gagcggcggc
    49681 atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg aatcaccgtt tatcatcgcc
    49741 gatgaaccga ccaccgacct cgacgtggta gcacaggcgc gcatcctcga tctgctggaa
    49801 agcattatgc aaaaacaagc gccgggaatg ctgctggtca cccatgatat gggcgttgtg
    49861 gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta aaattgtcga acagggcgat
    49921 gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc gcagcctggt ttccgctcat
    49981 ctcgccctct acggtatgga gctggcatca tgaccttact taacgtctgc ggcctttccc
    50041 atcactatgc ccacggtgga tttagcggaa aacatcaaca tcaggcggtg ctgaataacg
    50101 tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct gggacgcagc ggctgcggaa
    50161 aaagcaccct cgcgcggctg ctggtaggct tagagtctcc ctcacaaggg aatattagct
    50221 ggcacggcga atcgctggcg aaacttaatc gcgcccaacg taaagccttc cgccgtgata
    50281 ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc gcgcaaaacc gtgcgcgaga
    50341 tcctgcgtga accgatgcgc cacctgctat cactgaaaaa atccgaacaa ctggcgcgcg
    50401 ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt tctcgacaaa cgcccaccgc
    50461 agttaagcgg cggccagctc cagctcgtct gcctggcacg cgcgctggcg gtcgaaccga
    50521 aattgctgat tctggatgaa gccgtttcta accttgatct cgtgttacag gcgggcgtca
    50581 ttcgcctgct gaaaaagcta caacaacagt ttggcactgc ctgcctgttc atcactcatg
    50641 acttacgcct ggtggaacgc ttttgccagc gagtgatggt gatggacaac ggacaaatcg
    50701 ccgaaaccca ggcggtggga gataaattaa ccttttcctc tgacgccgga cgtgtgctac
    50761 aaaacgcggt attacccgca ttccccgtgc gccgtcgcac ctcagaaaag gtttaacgcc
    50821 aatgcaacga gtcaccatca cgcttgatga cgatttactg gagacgctgg acagcctgag
    50881 ccagcgtcgt ggttataaca accgttccga agctatccgc gatattctgc gtagtgccct
    50941 ggcgcaggaa gctacccagc agcacggcac acaaggtttc gcggtgctgt cgtatgtgta
    51001 tgaacacgaa aaacgcgact tagccagccg tattgtttcc acccagcatc atcaccacga
    51061 cctctccgtc gccacgctac atgtgcacat caaccacgac gactgtctgg aaatcgccgt
    51121 gttgaaaggt gacatgggtg acgtgcagca ttttgccgat gacgttatcg cccagcgcgg
    51181 cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat tgagttattt ctaaaacaag
    51241 gaaagaaaag atattaactg aacttaattt ataaaattaa gttcagttgc atagatagat
    51301 cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat gattcaaacg
    51361 ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg cagccgcatt
    51421 attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa tcttttctgt
    51481 acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc gcagggaaag
    51541 ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat aacctgacaa
    51601 gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa cagctggttg
    51661 cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac ccctatactt
    51721 agtaaccagt cacacacgct ttctacagcg cctttatcgg tatctggcga cggatgaggc
    51781 cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc tcttccttcc
    51841 ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt gatcccttga
    51901 ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg taaagcctgt
    51961 accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag tatgaagtca
    52021 agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca gtgcgatggt
    52081 gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga tttcgaatcc
    52141 ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg gcatggtcag
    52201 cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac cggaaagcat
    52261 ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg aacgcgctat
    52321 cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca cgcccagcat
    52381 aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca tcagcaccag
    52441 cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg ccatcatgat
    52501 ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt cacgctcgcg
    52561 gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga tgatcgccat
    52621 caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg tttccagcga
    52681 taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt caccgttgat
    52741 aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg catcgacgtt
    52801 cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag gcggaatatt
    52861 tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg cggtgatcat
    52921 ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg ataactgcga
    52981 ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg taacggtcgc
    53041 tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg ctttatcacc
    53101 gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat ggcgcatcat
    53161 ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat ggctatcagc
    53221 aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa cgttccgcgg
    53281 gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc aggcccttcc
    53341 agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa tgtgataatc
    53401 gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag cagccccatt
    53461 ccagtggcaa tgatgatata cagcagcgcc gccaaggtga gcgtcaggaa actgccttta
    53521 tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt cagcatcccc
    53581 agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt gggggtcacg
    53641 taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc gcttagcatt
    53701 gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg caggcttttt
    53761 acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg gctggcgggt
    53821 gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg cacgtaacct
    53881 tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag ttccacaggc
    53941 gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac cgtgatatcg
    54001 cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg ttcgataaag
    54061 taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg gtcgcggtcg
    54121 agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat tatcagcatc
    54181 aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg cagttccagc
    54241 gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg cggcgcgtgg
    54301 gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc cgcttcctgc
    54361 aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac cagttcctgc
    54421 ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc gcaacgttcc
    54481 gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg cgagagatcg
    54541 accatcaact gccagaacat atccctcgcc accggatcga caccagaagt aggctcatcg
    54601 aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg ctggcgaatg
    54661 ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa acgctcgctc
    54721 atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg ggcatgtaac
    54781 tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga catatagccc
    54841 acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag ccacgcctca
    54901 ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt accgcagccg
    54961 ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac gtgatcaacg
    55021 gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat ctctgcgttt
    55081 tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc ttgcggtaac
    55141 agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag ctcttcggcg
    55201 ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa gcgttcggcc
    55261 tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg gcgaatactg
    55321 tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt tggctcatca
    55381 aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt ttgcttcatc
    55441 ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt gctggtcagc
    55501 agctcattaa ttcgcgcttc ccgctccgct ttatcgtgac cgaacaggcg agcgaaaaaa
    55561 tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc ctgcggcatc
    55621 caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc gccacccagc
    55681 accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa taaactcgac
    55741 ttcccgacgc catccggacc aatcagcccg accatgcaac gggcctgaat atcgagagtg
    55801 atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag ttgcgcgaca
    55861 ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg aggtcgtcag
    55921 gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg gttttgacat
    55981 attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg aacatcagtt
    56041 tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca ctggcgacaa
    56101 aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc cgggcttcac
    56161 cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata tagacgtcgc
    56221 tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct ggctcggcaa
    56281 cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg atatctgcgg
    56341 caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga atgatattgg
    56401 tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct gattccagcg
    56461 cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca gaaatagccc
    56521 ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacagagtcc agttcggcct
    56581 ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc tccagcaaag
    56641 cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct tccagtcgct
    56701 gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga acaaactggc
    56761 cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata tccacttccg
    56821 tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc gggcgcaaca
    56881 gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac gccagatggc
    56941 gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac gcgtgttaca
    57001 actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga aacgacaaat
    57061 acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa cgggtagtaa
    57121 tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata agtgtgatct
    57181 ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcatttcacg cctgttcagt
    57241 tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga tagtaacatt
    57301 aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa tcaaaggcat
    57361 aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta tctctggccc
    57421 tgcaccaggg ttaatttggc aattatgcat gctgaacatt cttccagcat gtttactgtc
    57481 acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc caccatttcc
    57541 gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata gtgcttcaag
    57601 tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac aagagatagc
    57661 ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat taataatttc
    57721 cgctaattcg accggatcga gagcattggt catttcatca atagagacag ttatcggtcc
    57781 tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt
    57841 gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc
    57901 aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt
    57961 tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat
    58021 aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca
    58081 tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc
    58141 tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt
    58201 ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt
    58261 ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc
    58321 gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga
    58381 acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat
    58441 atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac
    58501 tgcaacatca ttagcattat catttactgt catattatta agtaaataaa caatgctgca
    58561 ccaattaata tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat
    58621 aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata
    58681 tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg
    58741 gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctggc
    58801 aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa
    58861 gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt
    58921 gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata
    58981 gttaacattt tatcttatga catacacctc atattcgatg gggaaataat tatattttgc
    59041 acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc
    59101 ctaaatattg tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga
    59161 aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa
    59221 acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccaaga
    59281 atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg
    59341 gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt
    59401 tggtctgaaa gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc
    59461 attgcttcta attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaca
    59521 tatatttgct aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa
    59581 agcaccgaga caataacatt gattttcttc atttcttatt attccgtgat gactggttaa
    59641 aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc
    59701 accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa aattgatttc
    59761 atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat taccctccat
    59821 atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct
    59881 atcgcctgta tgatccccaa ggaagataca aggggttatc gcatcgtcct ttaaaaaaac
    59941 atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt
    60001 attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa
    60061 taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac
    60121 agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat
    60181 cacaccacat tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa
    60241 cttatcctta ggaaacgtct ctttagagac atcccaacgc cagtcagctt taaaattacc
    60301 gctaagaaga gtaaagaaat tttcaggtat gatagaaagc ccattaactt tataaattat
    60361 gtctgattta tctccggaga aattaataaa ataataacca tccttacact caatcttaac
    60421 tgtaatggga gacgataatt ttatcacatg gttattgtct ggtataattg taaagataaa
    60481 cgtgttagta gtattatcgg caaaactatt atattcttga atatctatag atggctcact
    60541 attgtaaaac catattttct ccatctgaag actatgttca tttaccctcc agtgaccatg
    60601 ttgcaggaaa tagatgatat gcatgccaaa atcgttatct atttgcgaat cattatccag
    60661 cttatcaatt tcaactatct ttgaacgatg tgttttatca accttgctat ggtgttgatg
    60721 attattaact ttatctgaat gcttatcttt atggtttgaa gggatgtact taaatatatt
    60781 gtcaattgct ttcataaata acccgcgata tttttgacct gttagtaaaa aataaatgat
    60841 acatgtctga aagacatatt tacgacgata taaaacaata atacttatat gttcgctgat
    60901 tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc atccctggaa
    60961 gacaattgta aaaaaaaaca acagaataag aattaatctt aggataaata tttatttatc
    61021 atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta tagttatgct
    61081 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
    61141 cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtag
    61201 cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg tggctttgtg
    61261 ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt tctctggcgg ccatatgcct
    61321 ggcattattc actaccgcat ttattatcat ttcgcaactc acgcgcggcg ttaatacgtt
    61381 ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca tgatatgggg
    61441 ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg gccatgttat
    61501 ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt ccggtcactt
    61561 tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg tacaggctta
    61621 ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc tgctcggttt
    61681 catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg tcgccttggg
    61741 ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca caatcgtcca tcaaacacgt
    61801 aatacgttct catccaaaga gcactggctg tggtgttatt gggttatttt tctcggctca
    61861 gtcacggtgc tgcaggggat atacgtctta gtcagttccg atgcaagcgc ccgactggct
    61921 cccggcatta ttctcatttg cctcggaatg atctgttaca gcatattctc aaaagtctgg
    61981 ctactggcac tggtctggag acgtacctgt tcgttagcca acagaatacc gatgataccg
    62041 gtatttacct gtctgttttg cttgtttttg tcatcattcc tcgcggaaat cgcacaaact
    62101 gagctggact actttattcc atctcgggtt ctggttggat taggcgcagt atgttttaca
    62161 ttgttctcta ttgtttcaat attagaggcc ggttctgcga aaaaaaatta aaatttacat
    62221 aatcccagcg ttattcatat cttaacgctg gttttttacg aacttagtga aataatgtag
    62281 aatataaaat tataatttta tattggagca catgtgttaa tgatcaaccc cgggatacta
    62341 aaaatataga cattattaaa cttgcttagc ctcgcattgc ctttacctgt taaagatgca
    62401 tcgagaaaat tgagtctgaa gttctcatcc tttatctgat tttccagtcc cgaaactttg
    62461 gccttagcat tacctgagat ccgtccgtta ctatctattt tgtaggtaaa aaaatacgaa
    62521 tgggaaaatt tcctcggtgt atcttcatca gttaagccaa caatcgtaaa actaccgcta
    62581 ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt ataaagaatg atctgtttta
    62641 gcatttcttg ttaacgtcga cgtacattca agagacgttg tgctaccatc acgagccttc
    62701 cagagagcaa aaaaacacac ctgtagcaat gtgaaaacaa gtattaatat aaaatacatt
    62761 ttcttattag tcatataata gcacctctga acaaattgca taagtttcgt cattagagga
    62821 ttttatacat cttgataaat accgctgccc ttccccaagc aaataatgca tactatttac
    62881 atcatatata aaatttgcac ctggcacaca gggtaaatgc tttttcgaaa tttcagcggc
    62941 ctctatttga gatttagcga tgccaatctc gttaagattc tctgaaagat ataaatcaca
    63001 ggtgtcaatc ttacctgatt taaccaatgg ataatccgtt atactgtagg tatctttaaa
    63061 agccacggca aacatttcaa taataacaat tactaccaca agcgaaatca aaatgcgaaa
    63121 aagaggaata ctccttttgc gctctacttt ttcaacaaca tcttcttgta aatgagaatc
    63181 atcataagat tgtttactta ttgttatgtt ttcactgatg taaaagccta ctttgggtat
    63241 cgtaagaatc acattttctt caacaccaat atttttaaaa cttttgcgta acagggatat
    63301 atattggttc aatgtgttgt ttgacagatc aaatgaaaat ctctccaaaa cgctatcaat
    63361 gattgagttc ctgtcaaccg ctccaccatt actttccagt aacactgcca aaatacaccc
    63421 cgccgtagct ggaaaattta tctcatcctg ggtatttaca ttcgagagcg agcatttgtt
    63481 cggatcaaaa ataatcgcgt cattgataat gaaaaaacat ctccgctcca tgcaggcaac
    63541 aacacaaaat aagattttgt cattttagcc tattcctggt ggaactgaca caagaaacta
    63601 aaatacaaat ataaccctga aaaagtcata taaaacttat atccacagaa taaaaaatca
    63661 aacatgtcag ttttatcaat ataaagtatc gcaaggcgaa aagtgatgca agaatactcc
    63721 taagtaaatg aaagcgcacc acatgaaatt atcaacacat gtaatttcat cattaggtgc
    63781 tatcgaaaat aactcaggag ctacttaaga tgaataatgt cttactcatg agacccgatg
    63841 actcagaatt tatgatacct gatgactggt cagggctaaa cgcaaaaggc attacatacg
    63901 aacaagataa tagagtgaca gagtcttttc tgccttttaa atctttttat tgttcaccgg
    63961 tgtgcacgac agttgaactc atctcataaa ataattgttt tgctatgggt atccgctacc
    64021 ttctggaaaa gaaaagagac ttaaggctaa atcactttaa agccttctct cataaatgcg
    64081 agacaaaacc tgacgtgtta attatgaccg ggtgcaccgt cgctgatact tatagtatat
    64141 tctcacgcat tgttgaagaa aatataatga aaacgatgac acctatcgtc atcattactg
    64201 ggcatcactc agaagatctt tgttctctgg catcgaaact gggaggagtt aaaataatat
    64261 caccgacaga aaagattgca aaaatagaaa acgctatcaa tgatgctttt aacagcaagc
    64321 ctaaaaaaat aagattttta acagatatac tctctcctca acaaattgag accatgaaaa
    64381 tggttgcgca gggtagatcc gttaatgaga tttcagccgc aatgaatatt agcatcaaga
    64441 ccgtgtatgc acataaagtt cgtgctatca ataagctggg aattaagaaa aaagtttatg
    64501 aagcgctttt ttataaagcg ctaattcagt tcaactgatg taaaaatcac cctcgcttaa
    64561 taaataatat attctatgtc tttttttata agttaaaaaa ggcgggatat caaatcccgc
    64621 catgaaatta actaatcatc tgcattattc tgcgtagata taagcctggg tacggatggt
    64681 gtaagagtct ggctcaacaa cctgttcttt catatgggaa acgacgcgat aggcaccacc
    64741 tgttccagta tttttcagca tcatataacg cttaccgcca atctcttcgg aggcagctga
    64801 aggaggatcc tgctcaagcg ttgtcaggga aactttcagt ttatggtccg ggttattgtc
    64861 accagaaatt actgcatccg ctgaagtact gctctccgca taaatatcat ccgggaaagt
    64921 tacagcatag tcgtggctct gcccatcaga tgcgccaaca atacctgttg ccaaaggcgt
    64981 ccatgcagtt aacgtaccgc tatatacccc tttcactggt tgaatggtat tattaacaac
    65041 gataggcttg gtcaaagtaa gattagctga cgcttcagta ccctggctta ctaaagttcc
    65101 cgcctgagaa gcaaaagctg tccccgccag catgattgct aacaatgttt tcttcattat
    65161 attttctcca tgattttcat ttacactgca aaatgcagtt tctttctttg catttacgtg
    65221 ctatcgcgaa aatgcttcca ccaaataatg gaaacatctt cacaaaaata aattatgagt
    65281 ttcttaattt agaaactata aacatgtcgc cgctatctgg tataaccctg atgcactttt
    65341 cttcgcagga agttggtatt ttgcgataca ggattgatct ttagaataac cccatttcac
    65401 attaaactcg ccttccgcac tcaagccaga aacataagca ataccatctt cacctacaat
    65461 gccggccata ttttcagtgt ctttgctata actaatcaca gcgccgaagg ggatgggttt
    65521 accctctttc tgttttaagg ttactaatcc tttatccccg ctcttcactg ccaatgtcgc
    65581 aggcacaacg gctccagctg tcggtacaac caattgacta gtttgtggta agtcgtaatt
    65641 cttacctatc gttttttcat ccagaataac atcgtctctt c
//
LOCUS       DAJTYL010000006        64475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYL010000006 DAJTYL010000000
VERSION     DAJTYL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.006"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            27..152
                     /locus_tag="OO572_000382"
     ncRNA           27..152
                     /ncRNA_class="other"
                     /locus_tag="OO572_000382"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            272..613
                     /locus_tag="OO572_000383"
     CDS             272..613
                     /locus_tag="OO572_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6659506.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(604..1530)
                     /locus_tag="OO572_000384"
     CDS             complement(604..1530)
                     /locus_tag="OO572_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6659507.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1620..2618
                     /locus_tag="OO572_000385"
     CDS             1620..2618
                     /locus_tag="OO572_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6659508.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2615..2833)
                     /locus_tag="OO572_000386"
     CDS             complement(2615..2833)
                     /locus_tag="OO572_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6659509.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2835..4850)
                     /gene="ligA"
                     /locus_tag="OO572_000387"
     CDS             complement(2835..4850)
                     /gene="ligA"
                     /locus_tag="OO572_000387"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6659510.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4921..5907)
                     /gene="zipA"
                     /locus_tag="OO572_000388"
     CDS             complement(4921..5907)
                     /gene="zipA"
                     /locus_tag="OO572_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6659511.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6137..6898
                     /gene="cysZ"
                     /locus_tag="OO572_000389"
     CDS             6137..6898
                     /gene="cysZ"
                     /locus_tag="OO572_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6659512.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7083..8054
                     /gene="cysK"
                     /locus_tag="OO572_000390"
     CDS             7083..8054
                     /gene="cysK"
                     /locus_tag="OO572_000390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6659513.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8438..8695
                     /gene="ptsH"
                     /locus_tag="OO572_000391"
     CDS             8438..8695
                     /gene="ptsH"
                     /locus_tag="OO572_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6659514.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8740..10467
                     /gene="ptsI"
                     /locus_tag="OO572_000392"
     CDS             8740..10467
                     /gene="ptsI"
                     /locus_tag="OO572_000392"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6659515.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10508..11017
                     /gene="crr"
                     /locus_tag="OO572_000393"
     CDS             10508..11017
                     /gene="crr"
                     /locus_tag="OO572_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6659516.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11059..11910)
                     /gene="pdxK"
                     /locus_tag="OO572_000394"
                     /gene_synonym="thiJ"
     CDS             complement(11059..11910)
                     /gene="pdxK"
                     /locus_tag="OO572_000394"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6659517.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12015..12389
                     /locus_tag="OO572_000395"
     CDS             12015..12389
                     /locus_tag="OO572_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6659518.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12422..13145
                     /locus_tag="OO572_000396"
                     /pseudo
     CDS             12422..13145
                     /locus_tag="OO572_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13349..14260)
                     /gene="cysM"
                     /locus_tag="OO572_000397"
     CDS             complement(13349..14260)
                     /gene="cysM"
                     /locus_tag="OO572_000397"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6659519.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14394..15491)
                     /gene="cysA"
                     /locus_tag="OO572_000398"
     CDS             complement(14394..15491)
                     /gene="cysA"
                     /locus_tag="OO572_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6659520.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15481..16356)
                     /gene="cysW"
                     /locus_tag="OO572_000399"
     CDS             complement(15481..16356)
                     /gene="cysW"
                     /locus_tag="OO572_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6659521.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16356..17189)
                     /gene="cysT"
                     /locus_tag="OO572_000400"
     CDS             complement(16356..17189)
                     /gene="cysT"
                     /locus_tag="OO572_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6659522.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17189..18205)
                     /gene="cysP"
                     /locus_tag="OO572_000401"
     CDS             complement(17189..18205)
                     /gene="cysP"
                     /locus_tag="OO572_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6659523.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18509..19300)
                     /gene="ucpA"
                     /locus_tag="OO572_000402"
     CDS             complement(18509..19300)
                     /gene="ucpA"
                     /locus_tag="OO572_000402"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6659524.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19487..20287)
                     /gene="murR"
                     /locus_tag="OO572_000403"
     CDS             complement(19487..20287)
                     /gene="murR"
                     /locus_tag="OO572_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6659525.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20451..21347
                     /gene="murQ"
                     /locus_tag="OO572_000404"
     CDS             20451..21347
                     /gene="murQ"
                     /locus_tag="OO572_000404"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6659526.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21351..22775
                     /gene="murP"
                     /locus_tag="OO572_000405"
     CDS             21351..22775
                     /gene="murP"
                     /locus_tag="OO572_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6659527.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22955..23854)
                     /gene="yfeX"
                     /locus_tag="OO572_000406"
     CDS             complement(22955..23854)
                     /gene="yfeX"
                     /locus_tag="OO572_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6659528.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23950..24525)
                     /locus_tag="OO572_000407"
     CDS             complement(23950..24525)
                     /locus_tag="OO572_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6659529.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24586..25035)
                     /locus_tag="OO572_000408"
     CDS             complement(24586..25035)
                     /locus_tag="OO572_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6659530.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25022..25447)
                     /locus_tag="OO572_000409"
     CDS             complement(25022..25447)
                     /locus_tag="OO572_000409"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6659531.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25661..26530
                     /gene="amiA"
                     /locus_tag="OO572_000410"
     CDS             25661..26530
                     /gene="amiA"
                     /locus_tag="OO572_000410"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6659532.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26534..27433
                     /gene="hemF"
                     /locus_tag="OO572_000411"
     CDS             26534..27433
                     /gene="hemF"
                     /locus_tag="OO572_000411"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6659533.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27439..28491)
                     /gene="eutR"
                     /locus_tag="OO572_000412"
                     /pseudo
     CDS             complement(27439..28491)
                     /gene="eutR"
                     /locus_tag="OO572_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28537..29037)
                     /gene="eutK"
                     /locus_tag="OO572_000413"
     CDS             complement(28537..29037)
                     /gene="eutK"
                     /locus_tag="OO572_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6659534.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29050..29709)
                     /gene="eutL"
                     /locus_tag="OO572_000414"
     CDS             complement(29050..29709)
                     /gene="eutL"
                     /locus_tag="OO572_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6659535.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30891..31682
                     /locus_tag="OO572_000415"
     CDS             30891..31682
                     /locus_tag="OO572_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659536.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32402..32797
                     /locus_tag="OO572_000416"
     CDS             32402..32797
                     /locus_tag="OO572_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6659537.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32815..33078
                     /locus_tag="OO572_000417"
     CDS             32815..33078
                     /locus_tag="OO572_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659538.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33101..33643
                     /locus_tag="OO572_000418"
     CDS             33101..33643
                     /locus_tag="OO572_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659539.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33782..34069
                     /locus_tag="OO572_000419"
     CDS             33782..34069
                     /locus_tag="OO572_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659540.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34108..34314
                     /locus_tag="OO572_000420"
     CDS             34108..34314
                     /locus_tag="OO572_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659541.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34626..35111
                     /locus_tag="OO572_000421"
     CDS             34626..35111
                     /locus_tag="OO572_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6659542.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35092..35370
                     /locus_tag="OO572_000422"
     CDS             35092..35370
                     /locus_tag="OO572_000422"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659543.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35363..36823
                     /locus_tag="OO572_000423"
     CDS             35363..36823
                     /locus_tag="OO572_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6659544.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36857..37666
                     /gene="murI"
                     /locus_tag="OO572_000424"
     CDS             36857..37666
                     /gene="murI"
                     /locus_tag="OO572_000424"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6659545.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37785..38479)
                     /locus_tag="OO572_000425"
     CDS             complement(join(37785..38233,38233..38479))
                     /locus_tag="OO572_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6659546.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38998..40371)
                     /gene="eutA"
                     /locus_tag="OO572_000426"
     CDS             complement(38998..40371)
                     /gene="eutA"
                     /locus_tag="OO572_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6659547.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40346..40825)
                     /gene="eutP"
                     /locus_tag="OO572_000427"
     CDS             complement(40346..40825)
                     /gene="eutP"
                     /locus_tag="OO572_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6659548.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40838..41173)
                     /gene="eutS"
                     /locus_tag="OO572_000428"
     CDS             complement(40838..41173)
                     /gene="eutS"
                     /locus_tag="OO572_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6659549.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41466..43745)
                     /gene="maeB"
                     /locus_tag="OO572_000429"
     CDS             complement(41466..43745)
                     /gene="maeB"
                     /locus_tag="OO572_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6659550.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44034..44984
                     /gene="tal"
                     /locus_tag="OO572_000430"
     CDS             44034..44984
                     /gene="tal"
                     /locus_tag="OO572_000430"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6659551.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            45004..47007
                     /gene="tkt"
                     /locus_tag="OO572_000431"
     CDS             45004..47007
                     /gene="tkt"
                     /locus_tag="OO572_000431"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6659552.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47110..48153)
                     /locus_tag="OO572_000432"
     CDS             complement(47110..48153)
                     /locus_tag="OO572_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6659553.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48279..48854)
                     /gene="nudK"
                     /locus_tag="OO572_000433"
     CDS             complement(48279..48854)
                     /gene="nudK"
                     /locus_tag="OO572_000433"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6659554.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48922..50901)
                     /gene="aegA"
                     /locus_tag="OO572_000434"
     CDS             complement(48922..50901)
                     /gene="aegA"
                     /locus_tag="OO572_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6659555.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51107..52824
                     /gene="narQ"
                     /locus_tag="OO572_000435"
                     /pseudo
     CDS             51107..52824
                     /gene="narQ"
                     /locus_tag="OO572_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            53026..56139
                     /gene="acrD"
                     /locus_tag="OO572_000436"
     CDS             53026..56139
                     /gene="acrD"
                     /locus_tag="OO572_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6659556.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56238..56297)
                     /gene="ypfM"
                     /locus_tag="OO572_000437"
     CDS             complement(56238..56297)
                     /gene="ypfM"
                     /locus_tag="OO572_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6659557.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56678..57034
                     /locus_tag="OO572_000438"
     CDS             56678..57034
                     /locus_tag="OO572_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6659558.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            57038..58165
                     /gene="dapE"
                     /locus_tag="OO572_000439"
     CDS             57038..58165
                     /gene="dapE"
                     /locus_tag="OO572_000439"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6659559.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58193..58393
                     /locus_tag="OO572_000440"
     CDS             58193..58393
                     /locus_tag="OO572_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6659560.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58503..59201)
                     /gene="ypfH"
                     /locus_tag="OO572_000441"
     CDS             complement(58503..59201)
                     /gene="ypfH"
                     /locus_tag="OO572_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6659561.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59275..61290)
                     /gene="tmcA"
                     /locus_tag="OO572_000442"
     CDS             complement(59275..61290)
                     /gene="tmcA"
                     /locus_tag="OO572_000442"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6659562.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61305..62168)
                     /locus_tag="OO572_000443"
     CDS             complement(61305..62168)
                     /locus_tag="OO572_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6659563.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62336..63049)
                     /gene="purC"
                     /locus_tag="OO572_000444"
     CDS             complement(62336..63049)
                     /gene="purC"
                     /locus_tag="OO572_000444"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6659564.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63262..64296)
                     /gene="bamC"
                     /locus_tag="OO572_000445"
     CDS             complement(63262..64296)
                     /gene="bamC"
                     /locus_tag="OO572_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6659565.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64313..>64475)
                     /locus_tag="OO572_000446"
     CDS             complement(64313..>64475)
                     /locus_tag="OO572_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCR6659566.1"
                     /translation="HNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITDSGRETVR
                     AALKHAGLL"
BASE COUNT        15940 a        16829 c        15959 g        15747 t
ORIGIN      
        1 aaaaaagcgc cctaaaggcg cttttttgct atctgcgata ctcaaagatt cgaacctgca
       61 gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc
      121 gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgcttt
      181 tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt cttctaccga
      241 tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa cttcaattcc
      301 ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg cttcacaaat
      361 cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga ttgtagacac
      421 tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc aaatcaccct
      481 actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc aggagaaaga
      541 gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga ctcatatcga
      601 tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc
      661 tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac
      721 cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg ggctaccttc
      781 aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg ctaccatcgg
      841 gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc
      901 cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc
      961 tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat cgcgatggca
     1021 aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg
     1081 atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg
     1141 cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca
     1201 ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag
     1261 ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc
     1321 aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc
     1381 cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta ctgccgactg
     1441 gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa
     1501 aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat
     1561 caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta
     1621 tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg
     1681 cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag
     1741 ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg
     1801 gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga
     1861 ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg
     1921 gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa
     1981 tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga
     2041 ttttcctttc accattgctg gttggtctgg tgatgaatgt tcacggtgca aggggcagcc
     2101 ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt
     2161 cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc
     2221 agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt aatggtatct
     2281 ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg
     2341 ctatcgtgat tgtagttaac gtctttatgg cacgccgact gagcttcaat aaggcagatg
     2401 aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa
     2461 acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc
     2521 agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt
     2581 tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga
     2641 ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct
     2701 tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcaa
     2761 ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct
     2821 gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca
     2881 atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga
     2941 tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag tcgagcttta
     3001 gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac
     3061 gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc
     3121 aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat
     3181 gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc
     3241 gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg
     3301 atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta
     3361 accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt gcctgcggtg
     3421 agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt
     3481 ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct
     3541 ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca
     3601 cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct
     3661 tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga
     3721 atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag
     3781 gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta
     3841 atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc
     3901 gccgggaatt taaacgcaac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc
     3961 tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg
     4021 cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt
     4081 acccgatcgc tgaccggcac cccccacttt ttaaattgca gtaaacggcc aagatgagta
     4141 tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc
     4201 ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta
     4261 gcaaaaactt tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc
     4321 ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc
     4381 agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg
     4441 ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca
     4501 tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca
     4561 cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt
     4621 acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg ttgggtaggc
     4681 gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc
     4741 ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga
     4801 tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat
     4861 caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta
     4921 tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg
     4981 agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg
     5041 cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat
     5101 aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt
     5161 caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg
     5221 gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc
     5281 accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg
     5341 cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac
     5401 gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg
     5461 cgctggcgcg acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata
     5521 ggcaggctgc tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc
     5581 aggcggctgc tggaccggtt gacgcggctg cgcagacgca taaggcggta ggtactggtg
     5641 ttgcggcgac ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg
     5701 gtgaacacga acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc
     5761 gtcacgtttt gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt
     5821 acggctggtc cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat
     5881 taatatcaga cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca
     5941 cggcaaacat ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta
     6001 actttcacag cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct
     6061 aactgtccat tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa
     6121 ggagctcatc ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta
     6181 ttttgcgcaa ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc
     6241 gctgctggtc aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga
     6301 tgtctggatc ccgactttca tgagttacgt tccggactgg ctacaatggc tgagttatct
     6361 gttgtggcct ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat
     6421 tgctaactgg attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt
     6481 gactggcgct acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat
     6541 catgaaacgc gaatggcaaa aatttgtctg gtatctgccg cgcgcaattg tattactaat
     6601 tctttacttc atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag
     6661 cgcctggatg ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt
     6721 taaagagatg cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt
     6781 aaccagcctg tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg
     6841 tggcgcgacg gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca
     6901 atctaccggt tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta
     6961 ttccatcttg catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg
     7021 catattctct gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg
     7081 ccatgagtaa gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc
     7141 tgaatcgcat cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca
     7201 gcgttaagtg ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga
     7261 aaccaggcgt tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg
     7321 tagctgccgc tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac
     7381 gccgcaagct gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca
     7441 tgaaaggcgc aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc
     7501 tgctgcaaca attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg
     7561 agatctggga agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg
     7621 gtacgctgac tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct
     7681 ctgtcgccgt tgagccaacc gattctccag ttatcgccca ggtgctggca ggtgaagaga
     7741 ttaaacctgg cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg
     7801 atctcaagct ggtcgataaa gtcattggca tcaccaatga agaagccatt tctaccgccc
     7861 gtcgtctaat ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg
     7921 cagcgttgaa actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac
     7981 catcatcggg tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag
     8041 aattgcaaca gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt
     8101 ttttgtggcc tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg
     8161 tatttaacca gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc
     8221 tgaatcgatt ttatgatttg gttcaattct tcctttagcg gcataatgtt taatgacgta
     8281 cgaaacgtca gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc
     8341 gtctgttaaa aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta
     8401 gttccacaac actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat
     8461 taccgctccg aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg
     8521 cttcacttct gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt
     8581 taaactgcag actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga
     8641 agacgagcag aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc
     8701 cgggttcttt taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca
     8761 tccccgggta tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac
     8821 cggaaaaaaa tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt
     8881 gccaaggcat cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa
     8941 gaaaaagaag ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag
     9001 gaaatcatag ccctgattaa agataaacac atgacagctg acgcagctgc tcatgaagtt
     9061 atcgaaggtc aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg
     9121 gctgacgtac gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc
     9181 gacctgagcg ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa
     9241 accgcacagc tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact
     9301 tcccacacct ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc
     9361 gtcacctctc aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt
     9421 tacgtcaatc caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct
     9481 tctgaaaaag cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac
     9541 caggtagaag tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac
     9601 ggcgctgaag gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg
     9661 cccactgaag aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag
     9721 gcggttatcg ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc
     9781 ccgaaagaag agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa
     9841 gagatcctgc gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc
     9901 attatgttcc cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa
     9961 atctacaaac aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc
    10021 gtaatggtgg aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat
    10081 ttctttagta tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat
    10141 gatatgattt cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa
    10201 gttattgatg cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc
    10261 gatgaacgtg ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc
    10321 atttctatcc cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg
    10381 ttagcagagc aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag
    10441 ttcattgaag aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga
    10501 gaagatcatg ggtttgttcg ataaactgaa atctctggtt tccgacgaca aaaaggatac
    10561 cggaactatt gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc
    10621 ggatgtcgtt tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa
    10681 caaaatggtc gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt
    10741 ctctatcgaa tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga
    10801 actgaaaggc gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga
    10861 tactgtcatt gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc
    10921 ggttgttatc tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac
    10981 cgtgggtgaa accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc
    11041 gcccatcggc gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc
    11101 atgctgctgg gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc
    11161 gtttaacgcc ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt
    11221 ccctttcagg tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac
    11281 caccaccacc tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca
    11341 ttttaatgtg tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca
    11401 atttttaccg gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg
    11461 cagtaaatat tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat
    11521 atcgccaatc accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt
    11581 cagccactcg gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc
    11641 acgaagttgg cgcagcgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc
    11701 gtccggaatc gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt
    11761 cggcacggca aagacattca agccgttctg ttttatagca ggcaccgcaa tgctgttgcc
    11821 tacgcttccg tacactacct gcgactgcac ggcgacgata tccgcctgca gcgccctact
    11881 cttatcgtta aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca
    11941 tcgtattcca accgaaactt tacctgattc tggcagtcaa atcggctatt acaaaacaaa
    12001 gataaggtaa ttcagtgaag aaaatcattt gtctggccat tacgctgcta atgacactcc
    12061 ccgcttacgc gaagttaact gttcatgaag aagcccgcat caacgccatg ctggagggat
    12121 tagcacagaa aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag
    12181 cggtttccca cctgcgtctg aagctcggca atacccgtaa ccgcattgac actgccgagc
    12241 agtttattga taaggtagct tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga
    12301 tccccggtaa gagcgatgag aacgcacagc cttttttaca tgcgttaatt gcgcagacgg
    12361 ataaaacggt gcctgcggaa ggaaattaat ccgctttggt aaggcattta caggaggtaa
    12421 catgaaaaaa tgctttattt atcacgatga aaaatcgaat aaattttggt ggatagatta
    12481 cgaaggggat agtttagctg tcaactatgg caaggtaggt agtattggta aattccatac
    12541 aaaagaattc gataatgaag aacagtgtct gaaagaagcc agtaaattga ttgccgcaaa
    12601 aatgaaaaaa ggatatcaag aaactccaga ctttaacttc atggatctct actattttga
    12661 tgatgaagag attgggttac atcttaaaac gtcacaccca aacttccagt gccattttac
    12721 tgatccactt tatatgtgtt gctgggatga agaatctcct tttggtagcg atgaaggtgc
    12781 tgatgctcta aacgaacttg aaaatagcct ccgttctgga ctgtgctgat ttccctcaaa
    12841 tgttaattga aactatgtgg ggtatgaaat acatcgctat ggacagtatt cttgaagagg
    12901 atgtgcgtgc gcaattacta gccgatgaaa tgagctctat ccagagcaat atgattacct
    12961 acgcgactgc attcggtcag atcaaagtca tgggtaaaat ctcccataaa cttaaaaaga
    13021 tgggactcaa tgcactagcg cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg
    13081 gtcaggactc accaatactt caaaaaatga ttgatgacct tactgctttt ccacacgaaa
    13141 attaaaaact gcatttgtcg gcagcaacaa ctgccgacaa atcagattat tgacaaagtg
    13201 cactttgttt atgccggatg cggcgtaaac gcctgatccg gcataaaaat attgctaatt
    13261 caatatattg cagaaacatt gtaggcctga taagcggagc gcatcaggca gttttgcgtt
    13321 tgtcatcagt ctccaatgct attaatcctt aaatccctgc cccctgacta aaatgctctt
    13381 ccccaaacac tccggtggag aggtagcgat cgccacgatc gcagatgatc gctaccacca
    13441 ccgcaccagg gttagctttt gccacccgca gtgctccagc aaccgcgccg cctgagctga
    13501 cgccacagaa tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct
    13561 gatgaatatc cagcacctca tccaccagcg cagcgttgaa aatccccggc agatattccg
    13621 caggccagcg gcgaatgccc ggaatactgc tgccttcttc cggttgcagg ccaacaatgg
    13681 tcaccggttt ggattgttcg cgcataaagc atgaaacgcc ggtgatagtg ccggtcgtcc
    13741 ccatgctgga gacaaaatga gtgatgcgcc cgccggtttg ctgccagatt tccggcccgg
    13801 tggtggtgta atgcgcataa gggttatcgg gattattgaa ctgatcgagc agctttcctt
    13861 cgccacgatt cgccatctcc agcgccagat cgcgcgcacc ttccatgccc tgctctttgg
    13921 tgacaagaat cagttccgca ccataagcac gcatcgccgc acggcgttcc tggctcatgt
    13981 tgtcgggcat cagcaatttc atgcgatagc ctttcagcgc ggcaatcatt gccagcgcaa
    14041 tgccggtgtt accactggtg gcttcgatta agacatcacc cggtttaatt tccccgcgct
    14101 tttccgcctc gacgatcatc gaaagtgccg cacgatcttt caccgaacct gccgggttat
    14161 tgccttccag ttttaaccac acttcactgc cgttatccgg ccccattcgc tgcaacttca
    14221 ccagaggcgt attgcctatt gtttgttcta atgtactcac aatctctatc catacgtggt
    14281 gttgcctgat gcgacgcttg cgcgtcttat caggcctaca ggttacaaac cttgccatta
    14341 aaaagcccgg atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg
    14401 ctttgtgcga gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc
    14461 cgcgcatgtt gcagaccaac gaataaacgc tcgccatgct gcggggcatc gtcgccatgc
    14521 atcacgaccg tcagcggttc gttgtaccac cccagcggct gcaccactaa ttgggtgtag
    14581 tgacctttcg ggctggcttc cagtacctgt accggcagcg gcgaatcgag actggtacgg
    14641 cggctgatat ccacttccca cgggcgcagg aagagatcca ccggcccctg atacgcaggt
    14701 gtgtaaccca gcggccagcg atgcgcgcca acatggaact gcccgccgcg aatggttccc
    14761 tgcaggcggt tcacttcgcc cataaattcg agtacaaaac gggtcgctgg ttcgcgccat
    14821 acctgattcg gcgcatcagc ctgttcaata ttcccctgac tcatcaccac tacacgatcg
    14881 gctacttcgg tcgcctcttc ctggtcgtgg gtcacaaaca cgctggtgaa tttcagctct
    14941 tcatgcagtt gacgcagcca gcgacgcagc tctttacgca cctgcgcatc cagcgcgccg
    15001 aacggttcat caagcagcag aatttgcggt tctacagcca gcgcgcgcgc cagcgccaca
    15061 cgctgtttct ggccgccgga aagctgcgcc ggataacgat ccgccagatg cgcaagctgg
    15121 accatttcca gcaattttgt cactttcgct ttgatggctg cggcattcgg gcgctcgcga
    15181 cgcggcagca ccgtcaggcc aaaagtgata ttgtcgaata ccgtcatatg gcggaacagc
    15241 gcgtaatgct ggaacacgaa accgacttta cgatcgcgcg cgtgcaggcg actcacgtcg
    15301 gtgccgtgga agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatccgcagc
    15361 agcgtggttt tcccggaacc ggacggcccc agcaacgcga ccatctgacc agaaggaata
    15421 tccagtgaga tatcgttcag cacctgggtg cgaccaaaag acttcttgat attggcaatc
    15481 tcaatgctca tgatgctcct cctgctgcgc gcgtttttcc tgattctcca ggcgccactg
    15541 caacatactc tttaaaaaca gggtgataat cgccatcaac gtcaacagcg cggcggcggt
    15601 aaaggagccg acggtgttgt agtcctgctc caacaattca atctgtaacg gcagcgaaag
    15661 tgtttcgccg cgaatcgagc cggaaaccac cgacactgca ccaaactcgc caatcgcgcg
    15721 ggcgttggtc agcaccacgc cgtaaagcag cgcccagcga atattcggca atgtgacgcg
    15781 acggaacatc tgccagccgg acgcgccaag caaaatcgcc gcttcgtctt cctggctgcc
    15841 ctggcttaac atcaccggca ccagctcgcg caccacaaac ggacacgtca caaagatggt
    15901 gaccagcacc attccgggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc
    15961 gccgagcggg ccgttggagc cgtagaacag cagatacacc agaccggcaa ccaccggcga
    16021 tacagcaaac ggaatgtcca gtagcgtcag cagtaactgg cgtcccggga agttaaagcg
    16081 cgtcaccagc caggccagca gaatgccgaa caccaggttt accggtacgg caatcagcgc
    16141 gatcatcacc gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac
    16201 cggcatcagc cccttgctga atgcctgcac gaagatgtaa atcatcggca ccagcaggat
    16261 gaacgccgaa accagcatcc cgatgccaat cagaaaccat ttgccccagt taatcgggcg
    16321 cgcgtcataa cgcttcaatt gggtaacttc cgccattaat gacctaccac acgccgacca
    16381 aagcgacttt gcagagtgtt aattgagaac agcagcagca gagatgccgc gaggatcacc
    16441 gaagcaatcg cgctcgctgc cgggtaatca aactcctgta agcgcacaaa aatcatcagc
    16501 gacgtcactt ccgtcttcca cgcgatattt ccggcgataa aaatcaccgc gccaaattca
    16561 ccaagactac gggtaaacga cagcgccacg cccgccacca gcgccggaga aagctccggc
    16621 agcaccactt tgcagaaact ctgccagcgc gttgcaccaa gcgtttccgc cgcttcttca
    16681 tattccgggc ctaactcttc cagcaccggc tgcacggtac gcaccacaaa cggaatgctg
    16741 gtaaaggcca tcgccaccgc aataccaagc caggtatagg tgactttgat atcaaacttc
    16801 gccagccatt caccgtaaaa accgtttacg gaaaagagcg aggccagcgt taaaccggcg
    16861 actgccgttg gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcggcccgga
    16921 aagcgataac gtgttaaaat ccacgccatc agcagaccga aaacgccgtt aaaaatcgat
    16981 gccacaaacg ccgacagcag cgtcactttg taggccgcga ccacctgcgg gttggtgatc
    17041 acctcccagt actgcgccca gctcatctca gaaagctgca tcaccagcgc agagagcggc
    17101 agcagcaata tcaggcacac aaacagcaga ctggtgccaa ggcttaaagt aaagcccggc
    17161 agcacgcgtc ttgaagaaac tgcaaacatt acttacgccc cgccgctaac agcttgtcta
    17221 actcgccgcc gctggtgaag tgggttttca tcacttccgg ccatgagcca aatttgtctt
    17281 ccacgcggaa cagttcggtc tgcgggaatt tatctttcag tttgtccatg acttccgggt
    17341 tattcacacg gtagtaatag tcggtgatga tggtctgcgc ctgtgggctg tagagccagt
    17401 tcaggtaggc ttttgcggct ttttccgtac cgttggcctg cacattttta tcgacccacg
    17461 ccaccgggaa ttccgccaga atattggttt tcgggatcac cacttcaaag ccctgtgctt
    17521 catactgttt acggatgttg ttcacttccg attcaaagct aatcagcaca tcgcccaagc
    17581 cacgctcggc aaaagtggtg gttgcgccac gaccgccggt atcgaacact tcaacgtttt
    17641 tcaggaactg ggtcataaac tgttcggttt tggctttatc gccaccgtca gctttatccg
    17701 ctgcgcccca tgccgccaga taggtataac gcgcgttacc cgacgttttc gggttcggga
    17761 aaatcagctt cacgtcggag cgcaccaggt cgttccagtc gtggatattc ttcgggttgc
    17821 ccttacgcac caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg
    17881 actgccagtc agccgggatc agcttgcctt tgtcgtgcag gatttgtacg tcggtcacct
    17941 ggttataagt gacaacgtct gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg
    18001 acccggcatg agattgtttt atcgtcagtt tgtcgccgcc gttatctttt gcccattgct
    18061 gttcaaacgg cggattcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca
    18121 gcagttccgt tgcctgtaca tggcccgcca gcagcagaga agcgaccagc gcgagtgagt
    18181 tctttttcag taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag
    18241 ccatcatatt tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg
    18301 gaagttaaaa agggaataag aacttggttc aacaggcgta attgttgcag gcagtttgga
    18361 cacggacagc gcggagaaac cggagcgtac acatagtacg tgaggatttc gagcactgcc
    18421 cggggccaaa atgacaaatg aaatagcctg gtgaacttag ttcaagacga aatcctcccc
    18481 acacatgcag ggaggaaaca gaggtgaatc agataccgac gctaaccgtc tccggcagtg
    18541 tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca tccgatgcga
    18601 ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacacagc gggattgctt
    18661 ttgccatttc agtcagcacc gattctggat cttccgggtt cgactggcgg gcaatgcttt
    18721 ccgccattgg cgtgcggacg tatcccgggc agatggcgtt aacgcgaata ccggattgcg
    18781 cgtactccac cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt aaggcatacg
    18841 ccgtttcgcc aggatcggcc accatatcac cagtgactga agacatcatc acaatgcgac
    18901 catctttgcg cgcaatcatc tccggcagca ccgccttcgt gacgttccat acgcctttaa
    18961 tattgatatc aatatggaaa tcgcgatctt catcgctcat atcgaggaaa ctgcccagac
    19021 gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc ttcgcgcgtt
    19081 tgatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc gtacagcgat
    19141 gaccacgacc acacagttcg tccgctagct tttcgatctc aggggagata tccagcaaga
    19201 ttaggttcgc gccatgacgt gcaaaagttc tggcaattcc ttcgccaatt ccctgcaatg
    19261 cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc tccttgttaa
    19321 tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata ttgctgcgga
    19381 tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc atttcaggcg
    19441 ttgtgttagc tcactgctgc gctgaatcat tttcaatgac gccacatcat tgatgttgca
    19501 caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg
    19561 agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca
    19621 gcggagagtc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt
    19681 ctgcgcacaa gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc
    19741 cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg caggccacgc
    19801 gatagccaat tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc gatccgccca
    19861 ggcccgttat ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg
    19921 cgtagtcgaa caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcagtttgc
    19981 gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg
    20041 cattcgtttt ctcccggctg gcactgtatt cgccaattaa cgccatacgc aattcagtaa
    20101 atccttgcgc gccgagcttc tgggcaaatt tcacaatgct cgactggctg ataccgagct
    20161 gtttcgccat ctgccgtgaa gagacggatt tcagctcgct cactctggcc cgcagaaagt
    20221 cagcaatttt ttgctcgttc ccggtaaatt cactttccgc attgcgtatt ttcgtcaggt
    20281 acaacatctt ccactctcct gggctggcag caaaaattcc gcttaagttc ttatgacgct
    20341 cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat
    20401 gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaactag
    20461 aaaagatgat tactgaaggc tcgaacaccg cctcggctga aattgaccgc gtatcgacgc
    20521 tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcctgcc gttgatcgcg
    20581 tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc ggcggcggtc
    20641 gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat
    20701 gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg tttgattgct ggaggcgaat
    20761 atgccattca gcacgcggtg gaaggagctg aagatagccg ggaaggtggt gttaacgatc
    20821 tgaaaaatat taatttaacg gcacaggatg tggtggttgg cattgctgcc agcggtcgca
    20881 cgccgtatgt gattgccgga ctggaatacg cacgccagct cggctgccgc acagtgggaa
    20941 tttcctgtaa tccggggagc gccgtttcaa ccaccgctga gtttgccatt acaccgattg
    21001 taggtgccga agttgttacc ggttcttcgc ggatgaaagc aggtacagcg cagaaactgg
    21061 tgctcaatat gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga
    21121 tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa
    21181 acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact
    21241 gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc
    21301 tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag
    21361 agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg
    21421 ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg
    21481 atcccaatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc
    21541 aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg
    21601 gcgatgcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag
    21661 ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc
    21721 tgatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg
    21781 tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat tttatgaagg
    21841 tgttcagcaa aggtttgttc accttcctag tgattctggt gggctataac gccgcccagg
    21901 catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg ctcggttata
    21961 accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg
    22021 atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag
    22081 gcatggtgcg ccgctttatg ccggacgatc tcgacatgct gctgacctcg ttaatcaccc
    22141 tgctgatcac cgctacgctt gcgtacctga tcattatgcc gctgggtggc tggttattcg
    22201 aaggtatgtc atggctgttt atgcacctga acagtaatcc gttcggttgt gcggttttag
    22261 ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc
    22321 tcgcgttaat ggacagccag ggattcaaca gcttatttcc catcctttca atggcaggcg
    22381 cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcgctac
    22441 gcagtcaggt acgcggggcg attattcccg gcctgctggg cgttggtgaa ccgctgattt
    22501 acggtgtcac cctgccccgc atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg
    22561 gcggtttgtt tatcggattg atagcctggt ggggtctgcc gatgggctta aacagcgcct
    22621 ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc atccttccgg
    22681 caatggcggt ttatgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca
    22741 cgctctttgg ctgccgtaac gtcaatctgg actgatatta tgaaacggac aatgctctat
    22801 ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa
    22861 ggtagttgat caagtatatg cggcgttaaa gcagtaataa aaaggccagt cagtaatgtt
    22921 actgcctggc ctttaatcaa tgaatcagaa acgcttacag cgccatcaac ttgtccagcg
    22981 acggtgcgaa ataatagccg ccggttaccg gtttggtgaa acgcaacatc gcatcacgtt
    23041 tgccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc agacgcgcgc
    23101 agtaggcgca gaagtacaga ccgtgagtgc cactggcggt gccgtacggc aggctctggc
    23161 gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtgagg tgagaggttt
    23221 ccggacgttc gtcgccgtca atctcttcgt tggcctcttt ggtgcgcccg atcatcatct
    23281 cctgatcgtg aacgctcatc cggttgagct gcttcaggtt gtgctcccaa cgctgcacaa
    23341 acacatagct gccgccggaa tccacgccgt ctttgataac cgccacttcg cggcgcgtct
    23401 cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc tcttccaccc
    23461 aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct tccatcgccg
    23521 cctgagcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc aacacatcga
    23581 actgggtcgt cggcgcaagg cctttaccgt agcccggaaa atctttcagc tcttctgccc
    23641 caacgccgcc gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc gcaccaagat
    23701 gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt ttactggccg
    23761 cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc gcgcggcaat
    23821 gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat aagaatgcca
    23881 cgcaatccgt ggcattgttg agcgctattt tacgtgtttt ttcgcgacgg cggtttgttc
    23941 agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca gggtatcgtc
    24001 cgaaggcatt aacccttcag gaccacgcca ttccccgctg aactggtagc taatatgttg
    24061 actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt tttggcaatt
    24121 gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac cggtgtcagc
    24181 aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc cgttaatcac
    24241 catcgccacg ttgtcgcctt tcatcacttc aaaaaagcgc accacgttgc cgttcgtggt
    24301 tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcagcaatgg cttgttcttg
    24361 cagtggtgtg gacgccgtta attcacccac gccctgctcg ctcactttgg tggacgatcc
    24421 aaaccagttc cacgggttag cggcagacca gttaactgac gacatcgtgg aacagccggt
    24481 cagcatcaac ggcatagcgc ataacattaa acgcagcgat ttcatgtcac ttcctttggt
    24541 tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt ctttttcttc
    24601 attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca caatatccgc
    24661 cactaccagc gccagaccga taccgctaac ggattcaccg ttcagccaca gccacggttg
    24721 ccagcaaagt aaaaccacct gcgccaacag caacagaaaa tagagcacat gccaggtgcg
    24781 aggaaacgaa gcccgccgac cgctcaacaa aaacgccagc accgccggaa taccgggaat
    24841 cagccccagc cagaaattat cgtgatcggg ataaaacaga tttagcagcg cagtaccctg
    24901 ctcacgcgac gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa gcaatagcac
    24961 aagccagaag agcaagggta aacgcaggcg tccgtgcgca tcataatgga caggatggaa
    25021 ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg gcgtgttcat
    25081 atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg ttgatctgaa
    25141 ttttcgggca gccgcgagca atcagctttt tctccagccg gttaagcaac gcattggcaa
    25201 ttccacgccc acgaaactct ggatgcacgc caagataata agcagacccg cgatgcccgt
    25261 cataaccgcc catcaccgtt ccgaccactt caccgtttac ctcagcgacc aaaaacaaac
    25321 tgacgtcatg gttcatctta cgctcgatgt ccatttccgg atcgttccac ggacgcagca
    25381 agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga aatacgcgta
    25441 tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg tgaacggtcg
    25501 aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga gttgtcaact
    25561 attgaaatga aaagtaaaac aattctcaac agcaaaccgt cgtaacggat tacgcgatac
    25621 gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt ttaaaccact
    25681 aaaaacactc acttcgcgcc gccaggtgct gaaagccggt ttagctgccc tgacgttgtc
    25741 aggaatgtcg caagccattg ccaaagacga acctttaaaa accagcaacg gacacagcaa
    25801 gccgaaagcc aaaaaatctg gcggcaaacg tgtcgttgtt ctcgatccag gccacggcgg
    25861 aattgatacc ggagcgatcg gacgcaacgg ttcgaaagaa aaacatgtgg tgctggcgat
    25921 tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt gatgcgcgtt taacgcgttc
    25981 tggcgatacg tttatcccac tttacgatcg cgttgaaatc gcccataaac atggcgcaga
    26041 tctgtttatg tcaattcatg ccgatggctt taccaacccg aaagctgccg gtgcttcggt
    26101 atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt ctgaacgcga
    26161 aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc tattgcagca
    26221 agtactgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc ttggctcgca
    26281 tattctgaaa aagattaagc cggtgcataa actgcacagc cgcaacaccg aacaagccgc
    26341 gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct cgtttatcac
    26401 caacccggaa gaagagcggc tgttaggcac ggcggcgttt cgtcagaaaa tcgccacagc
    26461 gattgctgaa ggcgtgatca gttatttcca ctggttcgac aaccagaaag cacattcgaa
    26521 aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa cagtttctgc tcaaccttca
    26581 ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca gaatttgtcg aagatagttg
    26641 gcagcgcgaa gctggcggcg gcgggcgcag tcgggtgttg cgtaatggtg gtgttttcga
    26701 acaggcgggc gtcaactttt ctcacgtcca cggtgaggcg atgcctgctt ccgccaccgc
    26761 tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac tggtagtgca
    26821 tccgcataac ccgtatgttc ccaccagcca cgcgaatgtg cgttttttta ttgccgaaaa
    26881 accgggtgca gagcccgttt ggtggtttgg cggcggcttc gatttaaccc ctttctatgg
    26941 ttttgaagaa gacgccattc actggcaccg caccgcccgt gacctgtgcc agccatttgg
    27001 tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat tacttctacc tcaaacatcg
    27061 caacgaacag cgcggcattg gcgggctgtt ctttgatgat ctgaacacgc cagatttcga
    27121 ccactgtttt gcctttatgc aggcggtagg caaaggctac accgacgctt atttaccaat
    27181 tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag cgcaattttc agctctaccg
    27241 tcgcggtcgt tatgtcgagt tcaatctggt ctgggatcgc ggcacgctgt ttggcctgca
    27301 aactggcggg cgcaccgagt cgatcctgat gtcaatgccg ccactggtac gctgggagta
    27361 tgattatcag ccaaaagatg gcagcccaga agcggcgtta agtgagttta ttaaggtcag
    27421 ggattgggtg taactccctc acccccattc ccgcatccgc tgatgcaacg tcaacgacgg
    27481 cttctcggca aacagctgct ggtaatccgt ggcaaattgc cccagatgcc agaatcccca
    27541 ctgcatggcg gcgtctttta ccgttgtgct ttgcgaccac gggcttatca gttcgcggcg
    27601 tacgacgttc aggcgaatgc gtttcagcca cgcgtttggg ccaatgccta aaatagcgtg
    27661 aaacgcgttt tgtagcgtgc ggcgactgac atgcagttga ttacacaaat ccagcaccgt
    27721 caccggttcg gacatgtttt ccagcacata ttcacgggcg cgggaaagca accgacggta
    27781 actctgatga ctgatgcttt ccgccgtcac cattggttgc gcttcttcca gcatcgcccc
    27841 catcgccatt agcaaattat cccccagcac ttttcgcact gcaggctgat ggagattttc
    27901 cgggttctcg caaaacgtcg ccagcgcctg ttggacaaag ccccacagcg cggctttatg
    27961 ctgctctttc acttccagcg ccgactggct acgcagcata tgtaataccc gatccggatt
    28021 gtgcaaaaag ttagcctgac aggtgatgac atcttcagac agcactacgc ccaggatcgt
    28081 ataatcgtcc ggcgtactta attcaaactc ggttccaccg gggcgcgtcg ctatttctgc
    28141 gcttcccaga cattgcgaac cgataaatcc ctgctcaccg cgcgtcgccg gaatgccaaa
    28201 ccagaacgag ttcggccaga ccaggcacga ctgacgcagc gccagaccgg tgtattcacg
    28261 aaaaacctga atatcatcga gtagaatttc cgtaaattca ccatgaaact tgcccggatg
    28321 cagctgatcg taaatctgct gctaggcggt aatcgtcaaa gcatgttcat agacatccgt
    28381 tgtctgtcgt tgatgaacat tatccacttc gaccttcggc gtgagcttca ggttttcggg
    28441 taagggttca tgataaagat ggtgcaaatt ggctgtacgg gtctttttca tgatgttaat
    28501 gccgggtgtt gtaggacacc cggcacctcc gacaggttaa tggggcttga gacgataacg
    28561 actactgcgt ttacgtaacg tcccggcaga aaagagctgt tcgagcgcat ttctggcttt
    28621 ttccagcggc cagccaaagt gggcggccac ttctcctgcc gtcattccct gacatactga
    28681 tgttaacagc gccaacaact cgtcagcaga ttccgcgacg ataactggcg cgtcgggttc
    28741 ctttacgggt tgcttcggct ggcggttaaa gccagtaacc agccactggg tgtcatcgtc
    28801 tggccgaccg atctccttac ggctgatgac acgcccggta cgcatcgcgg cagcacaacc
    28861 tgcgtccagc gccgcacgac acgccgccag atcgccttcc accaccagcg ttaagcgacc
    28921 agggtcaagc acttcgtgac tgagcagacg aacgttagct gctttgagca tggcatccgc
    28981 tgcatctatc gcggcgacca tgccgtccac ttccagcaat cccagtgcat tgatcatcgg
    29041 caacctccgt tacgcacgct ggattggatt acgcgcgatt tccagcactg catcggtaaa
    29101 ggcgttacag gctgctttac acgcggcctg gctaccggtt aaaaatgctg ccgagtagtt
    29161 ggtttcagac ggtggcggga cgtaggtcac cagctgtacg tcggcagatt tcaacgcggc
    29221 atcaatgccg taggtcgctt ccagcggcgg tgccaccaga tacgccatcg gatcgccaag
    29281 cgtgatcccg gcggttgatg aaagataaga accggtacgc gaaaccacat gcgccaggaa
    29341 ggcggtgttt tctgcgtcat tggcccactg gaaagctgcg ccattttcaa tatgtgcaac
    29401 catcgcatcc agaccggcac gcacttccgc cgggtttggc ccaccaagca taatcagcac
    29461 ttcaccggca gtcggtgacg ggccgtgtgc cgctcccgca tacagcgagc gaccatatac
    29521 cacttcgacc atcgcttgtt tggtcgcttc gtcagccgca atgtaggtta cgtcgtcaga
    29581 atcggcagaa atcagcccga ggctacgaat atgcggcggc aattttagtt cacgcgcaaa
    29641 ttcagcgtta acggaggcaa tcacccgcat ggcggttacc gacggtcgaa tcaaatctaa
    29701 agctggcatg atgcctcctt atcgggtcat gttgatgccg gatgctttct gctccagcat
    29761 acgtttcgcc aaatccacaa tgacggcaca gcagcacgaa agagaagcaa aagtcagcta
    29821 cctggtacac taggcagcgg gtcagcaaag gggggcaaat gccccttttt tcatgtcaga
    29881 taatcagatc attacctcaa taaactaata atcacatcgc ggttacaacc agtttgatag
    29941 tgatagctca aacccagacg tggcgctggc tgcaagcaaa ataagccgca aaaatgcgac
    30001 cccatgttta ctagccgcaa aaatgcggtc ctatttgcca gaacctttta ttattcaatt
    30061 ggttagtgca agaaaaagcg tgattactgt tcaagaagta tccagaaagg tcatgatttt
    30121 gacgctgaaa aaaaataggt gggcattttt gcccactcac tagccgcaaa tttgcccact
    30181 cactagccgc atttttgcgg cccaaagtaa taaatacaga gaaataataa atacagatca
    30241 gcgccaagat ttttcgtctt ttcggcggaa tcatgagact agtgcttacg tcctgataac
    30301 ctccagagca gaccttaact tctgtgcagc aggactacgc tcattgccgc tcggctaggt
    30361 caaatcctca cgataaagct gttcggattg tgaccataat gttaaaggac tctttaaatc
    30421 attccttgac gaattaacta gctggcttaa gcggaatagc atgcaggata aaaacaaaac
    30481 cagcttagca gtggggagag cttcgcttcg ctcgtctcct ggggccaatg ctcaatgaaa
    30541 cgacgtaagg cacggagcgc tcaccatagg tagacacata aacatctgag agcgcacctt
    30601 ctatcgcgct acgcgctcaa taaaaaccaa cgacaccgcc acactgcaag accaatggtt
    30661 tttatcgcgt atacagacca gacaggaaac agcagcaaag cagcgtatag ccaggtgcag
    30721 acagcacact cacacgacgc acagcaggct taagtgatca ctcaggaaac tttatccccg
    30781 ccgccgatca ccgcgaacta tcatgaccgc gccaaattcg aaaggaatgg cccatagaac
    30841 gcgatgaaca cacataagcg ctacacttac ttaacgtcgc cacagagcga ttgagagcgc
    30901 ttaaaatcga tgcgatttca ttaaattttt ctcactcgcc tgaatggtgg cgatcaggac
    30961 tgatcgcaac attgccccgc ctcgatattg agcgcataat agatacatac attaagcgga
    31021 aaagagataa gtttattgtg aaaatcaggg ggttacatat catgcgcata tcagaacaac
    31081 aacggcagat cctcgactat ctggcaaaaa acgaatcaat caccgcacga caagccgcag
    31141 atctcgttta tggtggcaat gttacccgcg tccaggttga cgccgcccgc cgctcactca
    31201 tgacgatggt tagtaatggg ctaatcagga aacaaggccg cgctttcgta gctgaaagac
    31261 atatcaatca cgcttatagc caggacgttg aagaattgcg ccgccagatc ctttgggtat
    31321 tcagcaaaca gggtcaggag tatttccttt ctgaacaccc aaatttcgcc gccaaagagt
    31381 taacagtaag cggattgtcg tgcgcactgg attatcttgt cggtgaaggt tgcgttactt
    31441 acaagcagct atggcacgcc ctgaatacgc ttgtagaaga tggtttactc attgccacgc
    31501 gtgagaacat cgaatactgg ataatgccgc gttcaggtcg ccgcaatcat gcacggtata
    31561 tttcaggcaa cagatacaga acgccggact ttaatccgac acaggaagat cgggaccgtg
    31621 ccgaaatgat gtgcagggaa caggagaaaa gaattatgga taagttattt tcgatgcagt
    31681 gaacacaagc gggcgcaatg cccgttttta ttgccttatt ccacgcctga cgaatcaatt
    31741 tgatcacacc tgagaaaatg catagccaga agttaaggcc gttatattgc gattgggagc
    31801 gttttaaacg gtatcaattt aattaattct gatgtggtga tttgcaggca aaaaaaaccc
    31861 gccggagaag cgggaaatag agacatatcg ttgtagttat taacagatca ctttgaggtc
    31921 gagggcggct aaccctcgct aatattatcc cgctattacc ttgcgggtgc aaataatctt
    31981 ttattgcttt gatgatttaa taaatagcac tttttgctaa caaaaaccgc tgtcaatata
    32041 tggccaaaaa tgtcaacaga aaaaataaga tttataatat taagtatcac atgggtaatg
    32101 ggttcaggga atcggaatcc accgcctcac ggaaattcaa aatttgcttc ttctttcctc
    32161 cgtgggcggc ttctttttcg tatcgaggta tttatgaaag caataacatt agaacagttc
    32221 gcagcaatgg cgaatgtatc gaccagaacg ctgaggcgct ggctaaaaaa accatcaacc
    32281 ggagtccctt tgccatttac cccacgcggt actcgccaat tattctggga gcacgaatgc
    32341 aaagaatggt tgtacagaac agaacgataa taatcacgca tcacattttg aggtaaacat
    32401 catgaccaat atcgaaagat tagaacgctg tgccgatatt atgcgacgca gatggattta
    32461 tgaccctgaa gcgggtttat tgtttagccg tgaaacaaaa gacgttatca aaggcagcat
    32521 gaccaataaa gggcatttgg ttgtaacggt acatgaaggt aaattcaaag tgacactcac
    32581 ctatcagaaa gcctgctatg tatacacata cggcgcttat gacgagacat tatatgaagt
    32641 gcaccacgtt aatttgaaca aacaggataa ccgcatcaag aatattcgcc tgatgccgaa
    32701 agaaaagcac cgccggatac ataaaaacgt ccgcaaactt attcggctgg gccacacaca
    32761 atggcttcaa ccgcaattaa ttgaacaatc actataaaca caggagtaaa taccatgcat
    32821 tcattagaca gttattttca gcgcacgaca gcccctaaat cagccgccca ggaacgccgt
    32881 gaggaattcc atgagaaagt gatgcgttct gctgattata ttgcggacaa gttcgtggaa
    32941 acggttcgtc cacttgttga tgaagtagct gataaacttc agtctgaaat gccggaagat
    33001 atggaaggca ctgcaaagcg gcgactcatt tgcgaattaa gtcgtcgttt tggcgtgtca
    33061 ataagcgctt ttaaataatc gaaaaaaggg gggaaagata atggacgcat ttgaggtact
    33121 ggcaaataaa ggcgctgaac tggtagcaca gcgtgataaa accgccaacg aaggggaacg
    33181 cgcactgcta aataaacaga ttaaagcgat ccgtatggcc cagtttaaat taatcagtaa
    33241 tgaagttatc gaaacaatta aaccgcagat cccacaaatc gttgctgacg ctattaaagc
    33301 cgccggactg gagaaacgca ttgccgggct aaaaacgaac gcagagaaaa tggagatgta
    33361 caaacagagc ggtgctccga atcccgatga gtacttctca ccggatgttg agtttatggc
    33421 gcaggttgag gaacgtctgg gtagctgtct caccgaggaa cagcgtcgct attttgatgg
    33481 agtggatagt tcggcaggta ttgacctgaa ctcgtatttc ggtcgtgaaa ttgagcattt
    33541 tgatgccgcc ccgtctatgc cggagccaga accagaaatg gccaccgatc ctgaagctga
    33601 gcaacggaaa cgcgtattca atgcgatagg gattcggtac tgatcggata aaagaaagcc
    33661 gggaattacc ccggctgact taacaatgtg accaatgaac aaatcggaca agacaaacag
    33721 gataagcagt ctcgaaacaa tgttcgggaa ctatattagc acaagtccgg agaaaatcat
    33781 catgttggca ctacgcatca tgcagggcat cgctaagacc ctcgcagaac atgtcttaga
    33841 cctgaaacat tcgccactga gcaaacaggc catgaagcgc caaacgcttc gtctgtgggc
    33901 tgaatattcg cttggaacta tcaataaaat catcgacatg aaatcggggc cgagcaacca
    33961 gagcgcggag gaaatggagt tcatcaggcg tttaattctc attcgcagag acattcatag
    34021 ccagttacac agcgtgggaa tcgatattaa tgatgggacg ggcgattaat tttcggacaa
    34081 gtactatgcg cgcgcgagga gctgaacatg aagcaagata ttgcagacag gctggagata
    34141 ctcgaaggcc agcgggcaga agcgaaacaa ctacgtaagc aggccagaag agcgcacagg
    34201 aacaacgaag cagagttact aaccaaatac atcagtttca ccaattactg catctacgag
    34261 tgttacaagg aagatgcaga ggactggctg gattcattgc cagaacagta ctgagcagaa
    34321 taacaccacg tcatgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattt
    34381 agtgtgccac ttcagcatca ccgttgaaat cacaataacg atttttttgt aaatatgctc
    34441 tcacatatgt aggaatagac caggttggca ttgaaacaca tctaccatga ccataataaa
    34501 gtaatttttt gatatgttta tggttttata ttaattaaaa tatatctaaa tttgatagtt
    34561 ttaaaattgc aaaatccaga ttgtgtttgt aatgtttaat ctgattgtat tgagtggcgt
    34621 ttattatgaa aaataaatca ggtaattctt gttatagaga agcagtcgat attcatagct
    34681 cacttgagct ttcatttata ttgcataaat atcatttgcg tgtagatgag aagaaatctc
    34741 gaaatgtatt cagcgccata cgtgatgagc tcatccttga tggggattca cgacaaaact
    34801 tagctacttt ctgtcaaaca tggcttgatg atgaaatccg tgagttgctt aatttagcta
    34861 ttgataaaaa catgatttat aaagatgaat atccacaaat ggcggaacct gaaggtcgct
    34921 gtgtgcgtat gctttccgat tcgtggagtt tccccgattc gcgacacacc ttaggttgct
    34981 caaccattgg ttcatctgaa gctgctatgc tcggccgctt ggcgttaaaa tggcagtggt
    35041 gtaaaaaacg cgaagtacaa ggtaaatcaa ccgaacctga tttgcgatcc tgtgcaaata
    35101 tgctggcata aattctgccg ttactgtgat gtgaaattgt gtgaaattcc acttgatgat
    35161 catcacctga taatgaatgc taaagaggtt ttgaagcatg tcgatgaaaa cactattggt
    35221 gtcgttacga ctttaggtat tacttatttt tgtaaagcag attgcgttag tgtttttaag
    35281 ataagtgtta aagttaatat tagtttttat atttacatcg taattatatg tttctatttt
    35341 aaaaatgttc gagagtataa aaatgtctaa tgctaatatc gcacccaaaa gtaagaaaat
    35401 gacagtgggg acactcgcta tcatgaatat aactgctgta gtaagtctac gatttatacc
    35461 ttctgaagca gaatacggct taggtgccat tttttactat acctttgctg caattatgtt
    35521 tttagtacct gttgcctttg ttgcggctga gctagctaca acatatccac aaaaaggggg
    35581 ggcatttcga tggataggag aagcatttgg ggctcgctgg ggttttgtag gaatgcttat
    35641 gacctggcta caagtcatcc cctactttcc aacagtactg acatttggtg cagtctcggt
    35701 tgcatttatt gatgttgata tgggggtggc agaatcaatt gccagtaata aatggtatat
    35761 tttatttttc gtactatttg tttactgggg agctatttgt gttgcattac gtggcgttgg
    35821 tattttctcg caggtatcta agtggtgcgg tattataggg acaattattc cagctattat
    35881 ccttgttatt cttggtttct cctatctgtg gttcagcggt aaaccagcgg tgatcccatt
    35941 aggttggggt gatcttttcc ctaatatagc taacttccag aatattgttc ttgccgccag
    36001 catatttctt gcttatgcag ggatggaaat gaatgcggtc catgttaacg atttagataa
    36061 tccaactaaa aaatacccaa ttgcaattac catcgcttct ataggtacta ttgcaatatt
    36121 cctgctcagt actttagggg tcgcctttat tattcctaca gataaaatta gtctaactca
    36181 aagtttgttg gttgcctacg atatgctgtt cgaatgggct ggagtgccat ggcttggctc
    36241 tgtgatggca tttatgctag caattggcgt actgggtggg gtggttactt ggattgcagg
    36301 gcctaataca ggtgtattag cgatcgcgaa agctggttat ttacctaagt ttttccaaaa
    36361 aaccaatcgt catggaatgg ggcaccatct aatgtttgta caaggtataa ttgtctcagt
    36421 tctttctgtt acttttgtga taatgccttc agttcaagca gcttttcaga ttttatcaca
    36481 gttaacagtt atgctctatt tagttatgta tatgctcatg ttcgctagtg ctatctatct
    36541 acgacattct caacctgata caaaacgccc atatcgagcc cctgctttat ttttctgggc
    36601 aacacttgga ttcttagggt cttttctggc ttttgtatta agttttattc cgcccgagca
    36661 aatcccaact ggtagtccaa ccagctatgt tctttatttg ttagtattag ttgccctatt
    36721 tttattaatt ccaatattaa tttacgcctt ccgaaaatct gactggaaag atccaaactc
    36781 tgatttcgaa ccattttctt gggaaaaaag aactagtaat taaattttcc ataagctaca
    36841 aaagatgagg ttaaatatgt ttagtgctga atctccaatt ggtgtaatag actcaggtgt
    36901 cggtggattg acatcggtaa aggaaataat taatttgctt cctggtgaag atattatcta
    36961 ttgtggagat aatatgaatg caccttatgg caaccgttct gccgatgata tcatcagttt
    37021 gactaaaaaa atgctgactt ttttacagtc aaggaatgtc aaactagttg ctgtcgcatg
    37081 taatactata tcctcaactt tggaatccga agaatacagc ggatatgcta agagctttcc
    37141 gtttcctatc ttatccatca ttgaacctgc agtagaagat gtgattcgcc agcaatataa
    37201 aaatgttggt attatcgcta ccgagtttac tattaaaacg gggtgtcaca aagagctaat
    37261 aaaaaaattg aattcaacaa taaatgtatt tggagaacca agcaaaaatc ttgcaatgtt
    37321 aattgaggaa ggtaatctta atgctcctgc aatcttgaat gacatcaaaa aacatgttaa
    37381 ccatttaatt tccctccatc cagtaaatga aattattctc ggctgtactc actatccaat
    37441 tgttcagaat ttttttgaag cagttgcacc ggatataaaa tttattaatc ctgcacatga
    37501 tcaggctatc tctattaaaa atcacttggg acagctaaat ttattaaact cctcaaatat
    37561 aggaactttg caaataaata ctagtggaag tatggagatc tacaaaacgg tactagggga
    37621 attgagcatt acgaaacctc atacattcag tatcaggcag ttttaaattt atccggtaca
    37681 ccgtaaaatc ccgtccttct gaactgcacc ccaaaagttg gacaccaact gagtaaggtg
    37741 cattttatgg acaaacccag ttacatactg ggtagtgcat ccaattagta gaacatgtgt
    37801 ttttcgataa acgctccgat cactttttcg tggatctcaa ctgagcgtga gaagcagatt
    37861 gttttacgac ccaaccgctt aatgcgggtg cgtagcgtca gattattacg ctcaatgcgt
    37921 tgggtgaata ttttgccggt cagatgctta ttcttcggca cctcccggcc atagctgccc
    37981 cagtcatcgc tggtcagcat gccgatgttg aagggtgtaa gcagtgccag tagctcccgg
    38041 cacgtttgat cggttcgggg accaaaagtg taggccagta caccgcctgt tttggtgttg
    38101 tacgcgtacc cgagccaatg ttgccgggct ttactgccaa cgtagctcca ttgctcatca
    38161 agctcgcaga taaacgccac atcagcatgg gcaacaggcg aagaagttat tcgctttggc
    38221 gcgagttttt taaagtccgg atgacggtgt tactaccaat tttcagtgtc ctggcggtat
    38281 cgcgaacccc ggcaccatta aaggccattt cagttatcag ctctttcata cccggcttac
    38341 gtgcttgata agtataagtg aactgaaaca cacggtggca gtcacggcag cgaagtctgt
    38401 cacggccttt agggttctga ccatggcggt aaacctgagc tgactgacaa cggggacaat
    38461 gaatattaac gctggtcatg agataacctc aaaagcccgt attatacatc agattcaact
    38521 aattagaggc atcacccatc cagcattata aaacatacta acaataacta aattataata
    38581 atcacaactt acaatcaaat aataaatgca taagattaac aatgattttt tcgggttata
    38641 tttatctaaa aaatcagaac attatgtaac ttattatttt ctaagcgaaa atttattgtg
    38701 cggtcagact aacaaacttg cgtaccaccc gaagaacatg cgaagattta agtattcttt
    38761 cttttttggt ttttattttt cattttctct tttcttctct ttccggttct gttctggttt
    38821 tggttttttc ctttttattt ttattctttc ttcagggtta tgctaaaaca ccaaaacatt
    38881 tttcaccaca aaagcatcca atcatcttgc cagaccaatc accttaaccg gatacactgc
    38941 cggatataat cccactaaca cccagacacc aagcaccata cctgataact accgtattca
    39001 ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt
    39061 accaatgtcg atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag
    39121 ttgttgtagc tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac
    39181 caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac
    39241 cgtcagaacc gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacacgtc
    39301 agttttggga tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt
    39361 ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctccagcca
    39421 gattgtgctg ccagagagcg aaagggtatg tgcacccgcg ccaatcaccg tggagcgtac
    39481 ggtttgcgcc ggaaactgca cattcatctc acgcaggcgc ggatggtcat gcagcgccgt
    39541 tgccagaagt gggccaatat cggcaaaaca gaacgggttg gcgggctggt ggcgataaca
    39601 ttcgcccacg cctccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc
    39661 ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg tcaatcactt cgacaatcag
    39721 cgccgccatc cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcagt
    39781 gcctgcaccg aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg
    39841 cccctggctg tcggtttcca gcaggcgacc accgacgttg agacaggcag tgccgctgat
    39901 ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg ccgatatcga tattcagtac
    39961 ccgacacagc cgttgttcag aaagggtttg cgccccggct ccgtgaccgg cgatcacgga
    40021 ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc agcgactgtt tttccttcat
    40081 cactttttcc atcagctgtg cgaccaggct ttcggtaaac tggccttccg gcagctgggc
    40141 aatgatggtt tcgcggatgc gctggctttc ggttttgtcg gctggcacgc tggcgggcac
    40201 ggacgccgtt accgggatcg attcatcaca ttcggtgatg gtaaaaccca gtagatccgc
    40261 cacttcacgg gcttccgggg taataatgct ggcgcgcaga accacggaca ttgcctgttc
    40321 gccgcgcgcg tgtgcttcac gaatatcatt cgctgtgatg agttttttca cctgcttcct
    40381 cctgtttggt gagagatgcc agataatcca ccagttgctg cacgctttgc gggtcgtggc
    40441 tgtttagctc gaaaatcggc tcttcgaaac ccgtctcaag caataacttt cgcgttgcgg
    40501 cgacgtcagc atctggcatg tccgttttac tgatgacggc gatttgccgt ttgctgacgc
    40561 caatatccag caacccggca ggcaaccgac tttccgggtc atttgcgcca tggacataga
    40621 tcagcatgtc tacatcctgt agcgtggtaa ttaaggcgtg ataccagcgg ggatggctaa
    40681 aatattcacc cggcgtatca atatcgccct tatcattaaa ttccacggcc agtgtttttc
    40741 tggcgagggt ataattcccc tgcaacgcat taaataacgt tgtttttcct gccccgacgc
    40801 tacccacaaa cgcaattcgt ttcatgaatg cctcgtatta acttttggtc atttcacaaa
    40861 gcgtataatt taataagcga cctaaaccgc tgacggtttg tgataacgct tcttctactg
    40921 cgcccaccga gccataaatc accaatgcac cgctgaagcg gtcgagaaaa ccgatatgca
    40981 catcagcggc tttcagcgct aaatcaccgg cgatcatcgc cgtttcgccg ggagttagcg
    41041 tcataatgcc aattgcgccc gcatccggaa cgccgatttt cttcgccagt tcctcaccgg
    41101 gatgcgcgat gagatgcgcc agtgtgacct gtttgccggg cacaaattcc tgaatgatgc
    41161 gttctttatc catgagtcgc cgcctgtgaa aaactttcgt gtatcgtgac aatttttcgt
    41221 cagagtaaat aacaaaattc ggcaagcgcg tttaaaaggt gaggtagatc aataagaatg
    41281 gagagatatt tactgttatg aataaacttt catatgcgac gcatgaatat aaataaaata
    41341 atatcgagat attttatata ctgatttttt tattattcgc aaactcagta ataagctttg
    41401 ataatatatt tcaggaaaac cgcttaccct gaatattcag ggtaagcgtg agagttaaga
    41461 atgaattaca gcggttgggt ttgcgcttca accacggcca gcgccaccat attgacgata
    41521 cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt tcgccacgcc catcagcacc
    41581 ggaccgacag tcacaccttc cgagctggaa acgcgcagta agttgtaact aatgcgggca
    41641 gcttccatgt tcggcatcac cagaatattg gcagaacctt tcaaagggct gtccggcata
    41701 cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt gcatttcacc atcaatcatc
    41761 agatctggcg cacgatccct gaccagttcc agcgcctgac gcattttgct cgacgacggg
    41821 cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa cgcgcggctc aataccaaaa
    41881 cgacggacag tttctgccgc catcaaggtg atctccgcca gctcttctgc gtctggttcg
    41941 tcattgacgt aggtatcggc aataaaggtg ttaccactcg gcagcagcag cgcattcatt
    42001 gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat ttttcaccac actaaaatgt
    42061 tcatgataat cgcccaccgt accgcagatc attgcatcgg cttccccacg ctgaaccatg
    42121 atcgcgccga tcactgtcgg gttactgatc agcgcacgct gcgcctgttc ctgagtgaca
    42181 ccgcgacgct tcatgatctg gaagtattcg gtccagtact ctttaaagcg cggatcggat
    42241 tcgttattga cgatttcaaa atcaacgccc gctttgatct gcaagcccag tttctgaatg
    42301 cgcatttcga tcacgttcgg acgaccgata aggatcggtt tcgccagtcc cagcgttacc
    42361 agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt ccggcagaac tacgcgcttc
    42421 ggcgctttgc gagcctggga gaaaatcggc ttcataaaca ggttggtttt gtaaacgaac
    42481 tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg gacgcgtcgc cacgcccgac
    42541 tccatcgccg cttttgcgac cgcaggagcg atcttaacga tcaagcgcgg atcaaacggt
    42601 tttggaatga tgtattccgg accaaagctc agatcctgaa cgccatacgc tgaagccacc
    42661 acttcactct gttctgcatg ggccagttct gcaatcgcgc gtaccgccgc cagtttcatc
    42721 tcttcgttga tggcggttgc gccaacgtcc agcgcgccac ggaagatgaa cgggaagcaa
    42781 agaacgttgt tcacctggtt cgggtagtcg gaacggccgg tacagatgat ggcatccgga
    42841 cgcacttctt tcgccagcgg cggcagaatt tccggttccg ggttcgccag cgccaggatc
    42901 attggtgccc gagccatttt cttcaccatt tcctgggtca gcactttcgg gccggaacag
    42961 cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg tacgtttgcc gtcatccacc
    43021 accgcatatg cggctttggt ttccgccata tttggctcac ggccctgata gataacgcct
    43081 tttgaatcgc aaaccacgat gttatgtttt tgcagcccca gcgctaccag caggttcata
    43141 caggcgattg ctgcggcacc cgcgccggaa accaccatcc gcacgtcgga gatgtttttc
    43201 tccaccacgc gcaagccgtt gaggatggcg gcagtgctga tgattgccgt gccgtgctga
    43261 tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt tctgttcaat atagaaacat
    43321 tctggcgctt taatgtcttc gaggttgatg ccgccgaagg tcggttcgag cgcggcgacg
    43381 acttcaataa atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac atcaatccca
    43441 gcgaatttct taaatagaac gcccttgcct tccatcaccg gtttgcctgc cagcgcgcca
    43501 atgttgccta accccagcac cgccgtaccg ttagagatca ccgcaaccag gttaccgcgc
    43561 gcggtatatt tgtaggcttt tagcgggtct ttttcgattt caagacaagg tgcggcaacg
    43621 cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc
    43681 tggattttcc ctggaactgg aaattcatgg aaatcaagtg cactttgttt taactggtca
    43741 tccatttggt tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct
    43801 gcccacaacg tggcgcacat tattaccctg ccggagtcta cagactttga gcaagtccaa
    43861 actctcacca ttaatataat gttttggtaa taatcctata acactgatgt tacctgctta
    43921 atccagcaat accatgcctg tctgctatgc ttttttgatg cgtttagcga aatttctcag
    43981 aagtgtgaat taacgcactc atctaacact ttacttttca aggagtattt cctatgaacg
    44041 agttagacgg catcaaacag ttcaccactg tcgtggcaga cagctgcgat attgagtcca
    44101 ttcgccatta tcatccccag gatgccacca ctaatccttc gctgttactc aaggctgccg
    44161 gattatcaca atatgagcat ttaatagacg atgctatcgc ctggggtaaa aaaaatggca
    44221 agacccagga acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg
    44281 aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt tgatgcacgc ctctcttttg
    44341 ataaagaaaa gagtattgag aaggcgcgcc atctggtgga cttgtatcag caacaaggcg
    44401 ttgagaaatc acgcattttg atcaaactgg cttcgacctg ggaaggaatt cgcgcggccg
    44461 aagagctgga aaaagaaggt attaactgca acctgacgct gctgttttct tttacccagg
    44521 cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc gtttgtaggg cgtatttatg
    44581 actggtatca ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta
    44641 aatcggtgcg caatatctac gactactata agcaacacca ctatgaaacc attgtgatgg
    44701 gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat cgactgacta
    44761 tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc tccagtggta cgtaaattaa
    44821 tcccgccttc tcagacgttc ccacgcccag cacccatgag cgaagcggag ttccgttggg
    44881 agcacaatca ggatgcgatg gcggtagaaa aactgtctga aggcattcgt ctgttcgctg
    44941 ttgatcaacg caaactggaa gatcttcttg ccgccaaact ataaaccagc cacggagtgt
    45001 tatatgtccc gaaaagacct tgccaatgcg attcgcgcac tcagtatgga tgctgtacaa
    45061 aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc cgaagtgctg
    45121 tggaacgatt ttcttaaaca taatcctacc gacccaacct ggtatgatcg cgaccgcttt
    45181 attctttcca acggtcacgc gtcgatgctg ctctacagtt tgctacatct gaccggttac
    45241 gacctgccgc tggaagaact gaagaacttc cgtcagttgc attcgaaaac cccaggccac
    45301 ccggagattg gctatacgcc aggcgttgaa accaccaccg gcccgcttgg acaaggtttg
    45361 gcgaacgccg tcgggctggc gatagcagag cgtacactgg cggcgcagtt taaccagcca
    45421 gaccatgaga tcgtcgatca cttcacctat gtgtttatgg gcgacggctg cctgatggaa
    45481 ggtatttccc acgaagtctg ttcgctggca ggcacgctgg gactaggcaa gctgattggt
    45541 ttttacgatc acaacggtat ttccatcgac ggtgaaacag aaggctggtt taccgacgat
    45601 acggcaaaac gttttgaagc ctatcactgg catgtgatcc atgaaatcga cggtcacgat
    45661 ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg tgaaagataa gccgtcgctg
    45721 attatctgcc gtacggtgat tggctttggt tcgccgaata aagcaggtaa ggaagaggcg
    45781 cacggcgcac cactggggga agaagaagtg gcgctggcac ggcaaaaact gggctggcac
    45841 catccgccat ttgagatccc taaagagatt tatcacgcct gggatgcccg tgaaaaaggc
    45901 gaaaaagcgc agcagagctg gaatgagaag tttgccgcct ataaaaaggc tcatccgcaa
    45961 ctggcagaag agtttccccg acggatgagc ggtggtttac cgaaggactg ggagaaaacg
    46021 actcagaaat atatcaatga gttacaggca aatccggcga aaatcgctac ccgtaaggct
    46081 tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg agttgctcgg cggttcggcg
    46141 gatctggctc ccagcaacct gaccatctgg aaaggttctg tttcgctgaa ggaagatccg
    46201 gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat cgccaacggc
    46261 atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt tgttgaatac
    46321 gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc ggcagattat ggtttatacc
    46381 cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga gcaactggcc
    46441 agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcagat ggaagcggcg
    46501 gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat cctctcaagg
    46561 cagaatctgg cccaggtgga acgtacgccg gatcaggtta aagagattgc tcgtggcggt
    46621 tatgtgctga aagacagcgg cggtaagcca gatattattc tgattgccac cggttcagag
    46681 atggaaatta ccctgcaagc ggcagagaaa ttagcaggag aaggtcgcaa tgtacgcgta
    46741 gtttccctgc cctcgaccga tattttcgac gcccaggatg aggaatatcg ggagtcggtg
    46801 ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga ttactggtac
    46861 aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg gttacgggga atctgctccg
    46921 gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc aaaagcgcat
    46981 aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgtatg ttcccctcac
    47041 cctaaccctc tccccagaag ggcgagggga cggtccgtgc gctttttagg gccgcatctg
    47101 cgattttgtt taacgggtga tccacaacgt gggcctggcg tctggcccat gccagttatc
    47161 gcaggtaggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa agcgccagcg
    47221 cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca gttcacgcga
    47281 tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctggc cgttgttgaa
    47341 cggcaaacgc aaccgaaccg ggcgcgaggc tagcggcttt ttacgcgaca caatccatgc
    47401 caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagtg ctttatcatc
    47461 ggtcagcgca gtgacattca cctcacgacg taacggatca agcgagcagc gcagaccatt
    47521 catccgccag tttccataat ccagcaaatc gttacgttct tcgagtgaga gtggcgtcgg
    47581 cgttgggtta accaccgcga cctctttcag cgcaggcgcg ggcggtacgc tgagaggcgg
    47641 ttcgtcccct ttcttgatcc acgcggtttc actgccaacg cgcttttgct gagcatcaat
    47701 aaacaacaac gccgctttta agccgctcag agaaatggtc tgattgccgt cgcgtaaggt
    47761 gatcgccttc ccttcctgaa ttatctgcaa aaacgcggtg atggttgccg tatcatcggt
    47821 tactaataac catggtgaaa tccgccactt gtcaccactt aatgccaacg gctcaccatc
    47881 taatagcagc cgtggcgcta tctccccttc agacgcatcc ggcgacttca acccaccgcg
    47941 ctcaatacgt aaaacggcat cggtatgcgc cccagcgctg cggctcaggg tcatcaccag
    48001 tccattatga tcgcccgtat tgcgcgccac acaaaaattt tgattattac aggtgacctg
    48061 ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg gcaacaaagc
    48121 gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag aagttcaaac
    48181 aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga tttgtcggat
    48241 tgagccaata ataaacaacg aaatattatt gctatttttc agtccattaa atgtgacatt
    48301 tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt tttgatcatc
    48361 tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc aacaccgcca
    48421 ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat tagctcagtc
    48481 acaccgccag gcgacatata cagttcaaat aatttgcgca cttcgcccac ttcatagccc
    48541 gtctcttcaa tcgcttcttt acgaatgcac acttccggtt cgtcgttatc cagcagcccg
    48601 gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc gacacggaac
    48661 tgacgaatca gaaccacgct ctttttcttc gcgttgtaca ggaggatcgt cgcaccatta
    48721 ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg cgtgagatcg
    48781 taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt aatgagggtg
    48841 atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatattac gctgtgaagt
    48901 cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca gccagtcaat
    48961 aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat ccgcaccacg
    49021 cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt actggctttc
    49081 cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact ccagccacgg
    49141 catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca tgacaaattc
    49201 actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc caagacgcgt
    49261 gcgcaggaaa cgaattccgc aaacgtgacc ttgctcattc agctcaagcg ccaccggctg
    49321 gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt tcttcgagcc
    49381 tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc cgtggcgcag
    49441 cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga cgttaagtcc
    49501 ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct gtttagtgtt
    49561 ggcaatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg gtaaacccgc
    49621 tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt ccaaaagcaa
    49681 atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc ccatcgcgct
    49741 gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag gaatgccgaa
    49801 agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccgcca cgccattgcg
    49861 ggtcagaaca tccgcacagg ccagccctgc cggacctgcc ccgataatcg ccacccgctt
    49921 gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg cctgatctga
    49981 aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc tcatcgcgaa tagtacaggc
    50041 accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca gggtgttggt
    50101 ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt caatccactg
    50161 cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc cgaacttaag
    50221 gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat ggcagataaa tttcatcaaa
    50281 accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg gcgttgcctg
    50341 catttgttcg actttactca ttaccggcat ttcttgtgca gcggtactgg catgccacgg
    50401 ttgatgctcc tgacgtgcgg tgcgcaagcg gcgggatttc gccatgccgg agagtgcgac
    50461 gtcagtgacc agttgcagcg cgtccgccgg gcaattctcg acacaggcag gaccgttttc
    50521 gcgccctgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg ccgcgacggg
    50581 tgtcaggacg atttgcatcg taccaaacgg acaggccacc acgcaggatt tacagccaat
    50641 acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc cattagggca
    50701 gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac gttgctgctg
    50761 atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat gttgctcatc
    50821 attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt gctgactgtt
    50881 ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc ttattcttta
    50941 tatgagtgtt gttattaccc gacttacagg ggaaccggca atgttcatgt gcccagaata
    51001 agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga ctaaattgcg
    51061 tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga ttgttaaacg
    51121 acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt cgattctttc
    51181 cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg ctatcaatat
    51241 tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa gtggcagtcc
    51301 acaactcaat gcacatcgcc agttgtttca gcaggcactg cattcaccgg tattaaccaa
    51361 cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc tgaatgccaa
    51421 ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc aggccaatat
    51481 taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact acgctgaacg
    51541 caaaatgctg ctggtggtgg cgatttccct ggctggcggt atcggtattt tcacgctggt
    51601 cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc agctggttac
    51661 cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata ccagtctgcc
    51721 gaatgagctt ggtctgcttg caaaaacctt taaccaaatg tcgagcgagc tgcataaatt
    51781 gtacctttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg agaccaagcg
    51841 tcgtctggag gtgttgtatc agtgcagtgt tcccaggcgc tgaacaccag ccagattgat
    51901 gtgcattgtt tccgccatat tttgcagatt gttcgcgaca atgaagcagc tgaatatctg
    51961 gagctgaacg ttggggataa ctggcggatt agcgaaggga agccaaaccc tgagctgcca
    52021 atgcagattt taccggtgac aatgcaagag acggtttacg gcgaactgca ctggcaaaat
    52081 agtcacgttt catcatcaga accgctgctt aacagtgttt catcgatgct gggacgcggt
    52141 ttgtacttta atcaggcgca gaagcatttt cagcagttat tgttgatgga agaacgtgcg
    52201 accatcgccc gcgaattgca cgactcgctg gctcaggtgc tttcttactt acgtatccag
    52261 ttgacgttac tgaagcgttc gataccggaa gataacgcca ccgcacaaag tatcatggcc
    52321 gatttttccc aggcgttgaa tgatgcttat cggcagttac gcgagctgtt gactactttc
    52381 cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga gggaaatgct ggatacgtta
    52441 caaaatcaaa ccagcgctaa actgaccctc gactgccgtc tgccaaccct ggcactggat
    52501 gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa tgcgatgaag
    52561 cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg caatcacacg
    52621 gtttatatcc gtgataacgg gattggtatc ggtgaaccga aagaacccga aggtcattat
    52681 ggactgaata tcatgcgcga acgcgcggaa cggctgggcg ggacgctgac tttttcacaa
    52741 ccttccggcg gcggcacgtt agtgagtatt agctttcgct ccgcagaggg tgaggggaag
    52801 gggaacacgc cggctgaggt gtaaataatt accctcacac cggccctctc cctttaaagg
    52861 cgagagggaa aaacgtgccc atattgacct gtaagcgagt gtgtcagcaa tctcatgata
    52921 atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct acagtgaagc
    52981 aagtcaagcc tacaacaata cgcagaaaca cgaggtcctc ttttaatggc gaatttcttt
    53041 attgatcgcc ccatttttgc ctgggtgctg acaatcctgt tgtgtctgac aggtaccctg
    53101 gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa tgtgcgagtg
    53161 accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac ccaggttatc
    53221 gagcaaaata tgaccggcct cgataatctg atgtatatgt catctcagag cagtggcacc
    53281 ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga agccgtgcag
    53341 caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt gcaaaatcag
    53401 ggcgtgacgg tgcgtaaaac cggcgatacc aacattctga ccattgcctt cgtctctacc
    53461 gatggctcga tggataaaca ggatattgct gattatgttg ccagtaatat tcaggacccg
    53521 ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata ttccatgcgt
    53581 atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga tgtcactgat
    53641 gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac accttccgtc
    53701 gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac accagaacag
    53761 ttccgcgata tcaccttgcg ggtgaatcag gacggctcag aggtaaggct gggcgatgtc
    53821 gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa tggtaagcca
    53881 gcctccgggt tgggggtaaa actggcctcc agcgcaaacg aaatggcgac agcggagctg
    53941 gtgctcaatc gtctcgacga gctggcgcag tatttcccgc acggactgga atacaaggtg
    54001 gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa aacgctgctg
    54061 gaagctatcg ctctggtttt cctcgttatg tatctgttcc tgcaaaactt ccgcgccacg
    54121 ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt actttacgcc
    54181 ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg tgctggcgat cggtctgctg
    54241 gtggatgacg ccatcgtggt ggtggaaaac gtcgaacgta ttatgagtga ggaaggactc
    54301 actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact ggtcgggatt
    54361 gcgatggtgc tttcggcggt atttgtacca atggccttct tcggcggcac caccggtgcc
    54421 atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt actggtagcg
    54481 atgctcctca ctccggctct gtgtgccacg ctacttaagc cactgaaaaa aggtgagcat
    54541 cacgggcaaa aaggtttttt tgcctggttt aaccagatgt ttaaccgcaa cgccgaacgc
    54601 tacgaaaaag gggtggcgaa aattctccac cgtagcctgc gctggattgt gatttatgtc
    54661 ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt cttaccgctg
    54721 gaagaccggg gcatgtttac tacctcggta caattgccca gcggttcaac ccaacaacag
    54781 accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa agacaacatc
    54841 atgtcggtgt ttgccaccgt tggttctggc cctgggggta acgggcaaaa cgtggcgaga
    54901 atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac ctcgtttgcc
    54961 attatcgagc gtgcaacgaa ggcgtttaac caaattaaag aagctcgcgt tatcgccagc
    55021 agcccaccag caattagcgg tcttggtagt tctgcaggtt ttgatatgga gttgcaggac
    55081 cacgctggag cgggtcacga tgcgctgatg gcagcacgta atcagttgct ggcgctggcg
    55141 gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc tcgatgacag tccgcagttg
    55201 cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgatatcga cgatattaac
    55261 gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga tcgcggtcgc
    55321 gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga tgacatcaat
    55381 ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt cgcgacctca
    55441 cgctgggaaa caggctcgcc gcgtctggaa cgctataacg gttattctgc ggttgagatt
    55501 gttggggaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat ggaatcgtta
    55561 gtgaagcagc tgccaaacgg ctttggtctg gagtggacgg cgatgtcgta tcaggagcgg
    55621 ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtggt attcctgtgt
    55681 ctggcggcat tgtatgaaag ctggtcggta ccgttctcgg taatgctggt cgtgccgctg
    55741 ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga cgtttacttc
    55801 caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct gatcgtcgag
    55861 tttgctaacg agatgaacca aaaaggccat gacctgtttg aagcgacgct ccacgcctgc
    55921 cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg cgtattgcca
    55981 atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac tggcgtaatg
    56041 ggcgggatga tttcagctac tattctggct atttacttcg tgccgctgtt ctttgtgctg
    56101 gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag caataaaaaa ggcgacatgc
    56161 caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt cttgctgttt
    56221 atactttgtc tcaggaatta cttacgaagc ataacttcga taaagtcttt ccagttcccc
    56281 agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg gaaacaatat
    56341 accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg acaaacctta
    56401 tgtaaatact atggtcctgc ggtgataatt tgtatgtgat acacagcaac atttcgagat
    56461 attcatacgg catctaatac tgatttaatt ctggttaaaa tacagacaga taacaagatg
    56521 aatattctta atgtttacgt taaaaatgtt taatattatt taatagttgt taatttgaat
    56581 acttcgataa tgttatattt tctgataatc atttgcaggc aaaatgtttt cacccttaaa
    56641 tgagtattta ttctcataaa tcgaaaaagg attcattatg gttacacttt acggcatcaa
    56701 aaattgtgac accattaaaa aggctcgccg ttggctggaa gccaataaca tcgactatcg
    56761 ttttcatgat taccgcgtcg atgggctgga cagcgaatta ttgaacggtt ttatcaacga
    56821 attaggctgg gaagcgttac tcaacacccg tggtacaacc tggcgtaaac tggacgaaac
    56881 tacccgcaat aaaatcaccg atgcggcctc tgcggcggca ttaatgactg aaatgcctgc
    56941 aattatcaaa cgtccattgc tctgcgcgcc tggtaagcct atgctgctgg gtttcagtga
    57001 ttccagttat cagcaatttt tccatgaggt gtagtctatg tcgtgcccgg ttattgagct
    57061 gacacaacag cttattcgcc gcccttccct gagtcctgat gacgcaggat gtcaggcttt
    57121 gttgattgaa cgtttgcagg cgatcggttt taccgttgaa cgcatggatt ttgccgatac
    57181 gcagaatttt tgggcatggc gtgggcaagg tgaaacgtta gcctttgccg ggcataccga
    57241 cgtggtgccg cctggcgacg ccgatcgttg gatcaatccg ccgtttgaac ccaccattcg
    57301 tgacggcatg ttattcgggc gcggtgcggc agatatgaaa ggctcgctgg cggcgatggt
    57361 ggtagcagca gaacgttttg tcgcacaaca tcccaaccat acggggcgac tggcatttct
    57421 gatcacttct gatgaagaag ccagtgccca caacggtacg gtaaaagtcg tcgaggcgtt
    57481 aatggcacgt aatgagcgtc tcgattactg cctggtcggc gaaccgtcga gtatcgaagt
    57541 ggtaggtgat gtggtgaaaa atggtcgtcg cggatcgtta acctgcaacc tcaccattca
    57601 tggcgttcag gggcatgttg cctacccaca tctggctgac aatccggtac atcgcgcagc
    57661 acctttcctt aatgaattag tggctattga gtgggatcag ggcaatgaat tcttcccggc
    57721 gaccagtatg cagattgcca atattcaggc gggaacgggc agtaacaatg ttattccggg
    57781 tgaactgttt gtgcagttta acttccgctt cagcaccgaa ctgactgatg agatgatcaa
    57841 agcgcaggta cttgccctgc ttgaaaaaca tcaactgcgc tatacggtgg attggtggct
    57901 ttccgggcag ccatttttga ccgcgcgcgg taaactggtg gatgcggtcg ttaacgcggt
    57961 tgagcactat aatgaaatta aaccgcagct actgaccaca ggcggaacgt ccgacgggcg
    58021 ctttattgct cgcatggggg cgcaggtggt ggaactcggg ccggtcaatg ccactattca
    58081 taaaattaat gaatgtgtga acgctgccga cctgcagcta cttgcccgta tgtatcaacg
    58141 tatcatggaa cagctcgtcg cgtgatgagt ggttctgcaa gaggaaataa gcatggactg
    58201 gctggctaaa tattggtgga ttctggtgat tgtctttttg gtaggcgtcc tgctgaacgt
    58261 gattaaagat ctcaagcgcg tcgaccataa gaaatttctc gccaacaagc cggagcttcc
    58321 cccgcatcgt gatttcaacg ataagtggga cgatgacgac gactggccga aaaaggatca
    58381 accgaagaag taagctgagt gctcaaccgt aggccggata aggcgttaac gccgcatccg
    58441 gcaatggtga acgatccctg atgcgacgct cacgcgtctt atcaggccag gtaatctgtc
    58501 ttttacatca tttcaatcac atcatcatcg ccaggcttac cgccacttag cgcttggtcg
    58561 aaataatgtt tcggaatggt ataacgcaag tgatccaggg caaattgcat actgcgattg
    58621 tcgatcgcat ggcctaaatc ctccacgata tccagcgtca cgtcaccacc ggcactgatt
    58681 aacgcttcct gcgccgcaac tgcatgcgcc agatcgataa ccggatcttc accaccatga
    58741 atcaagtgaa ttgtcgtggc tgtagacgcg gtctccggca ggctcgcata gcgtccgtta
    58801 aaggcaatga cgcgcgaagc aaggcccggt tcggctttaa tgctctccag cgccataatc
    58861 gcgccttgtg agaaaccaat tagcgccgtg gcgttagccc ctactccgct ctgtttctgc
    58921 cagtagcgta ccgtttcaat aaacgtcggc atgatggcat ccacacgcgc ctggcgatta
    58981 tcttccgtaa taccctgcac tgaaaaccac tgacgccccg ccggattacc gctcggctcc
    59041 gcgccaccga cactcaccac cagcgcatca ggaaacagcg gtgcaaacca gctgcctatt
    59101 tcccccattg ccaccgggtt atccccgaca ccgtggaaaa gcagtaacaa ctgttgtgca
    59161 ggtttatccg ggctttgaac aacaaaatgg tcatgtttca tggcgaactc cttaactgat
    59221 gtcattaatt ttacgccgct gactcacaat gaccatggga gaaaattgaa ggagtcagtg
    59281 aaaaaattgc cattgcgtta tgcgatcgcg cagacgctca gtgcgaacct catttagtgc
    59341 gaacagcgcc tgcgcggcct cttcccgctg gcgaaccagt aacatcttgc ggcctgaaag
    59401 tttaagtgtg gtacataact gcgcgtcact ggcgtttttc tgtaaacgcc cacgcagcgc
    59461 cggtaatgcc agttcactgg tttgtagcag acgcaataag caacctaacg atgttaatag
    59521 cggacgatga gcgaaagcaa aaccggccag ttcaagccag tcgtcgtcag aaaggatggc
    59581 atcgtttagt ggatcaacag gaagcatttc gccattccac tgcgcgagag cttgcgcatc
    59641 gcgacgtaaa cggtaatgct cacgttcagc cagctgttta cccgcatcac tcatcggtaa
    59701 cagcgccatc gccgtatagc aaccgctgct ggcttcccga tgattaccca tccgcaccag
    59761 cacaaaaccg cagcgttgcc agaaacgcca taactcccca gtgtaaccaa aactcaccga
    59821 aagatagtcg aggtcatgcg tatattgcaa agctccagca ataagttgtc gccctgtgcc
    59881 ttcccgctga cgagccggat gaactgctat ccggctaacc cgccttccgc gcaatgtcgc
    59941 cgccagtgga ttgctgccgt gcgccgccag cgactgggcc accagattac cccgcgggcg
    60001 acgaaaacct gcccataccg cctgactgag ttcttgagat aatccgccct catccaccag
    60061 ccacaacgcc ccggcaatct cgttttcgcc agccgcctgt aaaaaatgtt gccctggtgc
    60121 atccatcatc cggcgtaaat ccagcggcga ggtccggtag tgcgcaccag acaataactg
    60181 ataaaccttt aacggcgttt ctggctcgct tcgccataac gtttgttcaa atgcggaaat
    60241 gacgatattg ccttgtggtg tatgggtgaa gttttcatcg ttaaaaacca gtgcctcact
    60301 aaccattttt tccagcgggc atccctgtgc ccagcggatc ggctgttgca gttcaaaacg
    60361 gtgtaaatgc ggaaagcgag cgcaaaattt cagcaaaaaa ccacgtccgg taccttcgta
    60421 gccctgcacc gtagtggtta acaacgttcg aggaaaacgc gataccagtt gatgcaacaa
    60481 cggcgcaggt atggctgcgg cttcgtcgac caccagccag tcggcttgct catcgctggc
    60541 taacaaggca tccggtgcaa taaagcgaaa cttctcgccc gcaaattgtg ccagtacatc
    60601 cgttgccgct tttgcgggcg cggtgacaat cgcactgccc gcaatacgag aaatgagttg
    60661 ccctgccaac gccgacttac cgcgcccacg cgcagccgtt accgctgcca cgcccggcgg
    60721 catggtcagt agctgctgta agagttgctg ttgttctggt tgtggtgcgc cggtcgcggg
    60781 gtgccagtca gtacggggag taaaatgcgc caacgagaac ggctggtttt gccgccagag
    60841 gatagcgtcg ttatccgccg taagtacgcg tttgagatgc tggacaaaat gcggcgtcgc
    60901 aatagggtca gggcaatcac tccagcgcag cgagtcggca tcaggttggt tttcccactc
    60961 ttcccataca gggagtaaca aaaccagcca gcttcccgct ttcaacgttc cgctaagtgc
    61021 tgcaaaggca gcggcatcaa agccgtggcg ggcgtcgaat accgcatgcc ggaactcgcg
    61081 cccaagtaaa gtttgtagcg ccgagggtga acagtggttt tcagcatcta gctgcggcga
    61141 aatccacagc cagtcgccag gtaaggcatc acgcaactta agagcatgat caaaacacca
    61201 accctcttcc ccgctcaaca ccagcaagcg gcagatccct tcacgtttca tttgcgctgt
    61261 taatgtgtaa agcgcagtca gttcagccat ccctgccccg gaaattaaat gcttttacca
    61321 aaagtattgc attgtgccgg atcgccgctg tcgaaaccac gtttaaacca gctgtagcgt
    61381 tgctgagaag tgccatgtgt gaaactgtct ggtactactc gcccctgact ttgctgttgt
    61441 aaacggtcat cgccgatggc ctgcgccgca ttcagcgcct cttccagatc gccagtttcc
    61501 agaacgcctt gctgctgcat actatgcccc cagacaccgg caaaacagtc ggcctggagt
    61561 tccatacgca cagataagcg gtttacttcc gcctgcgtcg cgttttgttg cagttgacga
    61621 actttcggct cgatgcctaa cagtttctgc acatgatgac cgacttcatg ggcgataacg
    61681 tacccctggg caaaatcgcc atccgcgcca agtttgtctt tcatgtcatc atagaaggag
    61741 agatcgatat aaactgtgcc atccgccggg caatagaacg gccccataat ggactggccc
    61801 gccccgcagc cggtgcgcgt cattccacgg tacatgacca gcttcggttg ctgataggtc
    61861 ttacccatct tttcgaactg ttgtccccag gtgtcttccg tggttgcctg aatcaccgag
    61921 gtgaattttg cggcttcgtc ttcattaggg ctaattgacc gcgttgattg ttgttgagaa
    61981 accggctgcc cggtcatcaa cccggttaaa tcaacgccat agtagcctgc aaccagcacg
    62041 actatcagta aaatcagccc gcctttaccg ctcggcagac ggaaaccggg accgcccatt
    62101 gacggaccgc cagagctgtt gcgcctgtct tcaacattgt cactttcacg tcgcccttgc
    62161 caacgcatag atacctcaac aatatattca ttatagaaat gatcgtaggt ggttcagagg
    62221 aagattacca caggaaagcg ggatgaaatg gcggaggaat gagcgttcaa tgcatatatt
    62281 ccaagagcgg ttgtcttgcc gtgccagctg cacggcaaga tgatgaacag aaaaatcagt
    62341 ccagctgtac acccaggcgg cgggcgacgg cttcataggc ttcgatcagg ccaccgaggc
    62401 tctggcggaa acggtctttg tccattttct ccagtgtttc tttgtcccac aggcggctgc
    62461 cgtccgggga gaactcatcg cccagtacca cttcaccttt gtacagacca aactccagct
    62521 tgaagtcgac cagaatcaga ccagcatcat cgaacagttt tttcagcacg tcgttcgctt
    62581 tgtaggtcag ctctttcata cgcgccaggt tctctttgct cacccagcca aaggtttcgc
    62641 agtaagattc gttgaccatc ggatcgtgca tggcatcgtt tttcaggaac agatcgaaca
    62701 gcggcgggtt cagctcaata ccttcttcga ttccaagacg tttcactaga gagccagcag
    62761 cacggttacg cacgacacac tcaaccggca ccatatccag ctttttcacc agacattcgg
    62821 tatcggagag cagacgctcc atttgagtcg ggatacccgc ttcagccagt ttgctcataa
    62881 tgaagtagtt gaacttgttg ttcaccatac ctttgcgatc aaactgctca atgcgcgcgc
    62941 catcccctgc tgacgtatca ttgcggaatt cgagcaccaa caggtccggg ttttccgtgc
    63001 tgtatacggt tttcgcttta ccacgataca actcagcttg cttttgcatc tttatcactc
    63061 ctgggtgtga attaacgttt taaaatcttt tgctgtctgg tgtgccggat gttttgtcgg
    63121 atgcggcgtg aacgccttat ccgaactacg tccgacaaac aaatttcgtg cgaattaccg
    63181 ctaaaatcgc gttttcctgc cgacgcacac gtttgcgtat catatcagaa aaaagggccg
    63241 gatgattcca gccctgtatt tttacttgct aaacgcagcc tggaagacag ctaccagcgc
    63301 gtcgttctga ctctgagtca gagtatgacc tttcggatcg atgaactgta ggctgctgcg
    63361 gttatctaaa tcgccaacct gcagtttata gtcaccggat gccaggcctg gatcgctcgc
    63421 gcccagttcc tgccagtcgc tgtcagacag cggcttataa gttacggcca tgttgccctg
    63481 cgaacgggtg ctgtcggtca ctttcatgcc cactttttcc agcgccgctg gcagacgttg
    63541 ccaaaccaca ttgaacggcc cgcgtacgac cagcattggt aaaccggtgt catcagctgc
    63601 actttgtacg tccatagtgg tggaggcacg attttgcgca gcgttcgcgg cgtcagtggc
    63661 agatttatcc agaccggcgg aaataacgtt catcatctcc gtgctgtaac gctgcatgga
    63721 agccgcgtct gcaaccggtt tgcccgcctg ttccaggttc agcagtttaa ccgtaaccgc
    63781 ctgctgataa ccctgcggct taacagagat ttgataacga ccacgatact gctcgtcttc
    63841 gtccagacgg ttccattgta cccaatcggt ggtcagtgtc tgaccagcat catcacgttg
    63901 ggtgatggtg tagtttttcg cctgcagcac gctaaccacc tgcggccaca gagtattgcc
    63961 acgaccattt tccaccagca atgaagcggt atcgcccgtg aactgggtac gcgcgccaga
    64021 aaccagtgcc aacggctggg ctggtggacg aatgtccagc gccttaccga cagcaccact
    64081 accgttggtc accgggattg cataatcacc ggaggtcacc ggcaaaatca ttccagccgg
    64141 ggcatgaagc tccgcaagcg gtgccgcttc caggtaggct tcatcaccac tgacctgacg
    64201 cttatagcgt gagtcagaac tacaggcagc gagtaataaa acaagcgaaa cacccgcaac
    64261 ctttgccagg cgcgactttt gaacagagta agccatcaaa tctccctaaa ctttacagca
    64321 aaccggcatg cttaagcgcc gctctgaccg tctcacgacc actgtcggtg attggtgtca
    64381 ttggcaggcg cagcgtatcg gtcgccacaa gacccagttc cttacatgcc catttcaccg
    64441 ggattggatt gggttcgaca aatagtttgt tgtgt
//
LOCUS       DAJTYL010000007        63580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYL010000007 DAJTYL010000000
VERSION     DAJTYL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.007"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..816)
                     /gene="cra"
                     /locus_tag="OO572_000447"
     CDS             complement(<1..816)
                     /gene="cra"
                     /locus_tag="OO572_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6659567.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIAT"
     gene            complement(898..984)
                     /locus_tag="OO572_000448"
     CDS             complement(898..984)
                     /locus_tag="OO572_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6659568.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(996..1487)
                     /gene="ilvN"
                     /locus_tag="OO572_000449"
     CDS             complement(996..1487)
                     /gene="ilvN"
                     /locus_tag="OO572_000449"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6659569.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1490..3214)
                     /gene="ilvI"
                     /locus_tag="OO572_000450"
     CDS             complement(1490..3214)
                     /gene="ilvI"
                     /locus_tag="OO572_000450"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6659570.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3333..3377
                     /locus_tag="OO572_000451"
     CDS             3333..3377
                     /locus_tag="OO572_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659571.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3532..4476)
                     /gene="leuO"
                     /locus_tag="OO572_000452"
     CDS             complement(3532..4476)
                     /gene="leuO"
                     /locus_tag="OO572_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6659572.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5135..5221
                     /gene="leuL"
                     /locus_tag="OO572_000453"
     CDS             5135..5221
                     /gene="leuL"
                     /locus_tag="OO572_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6659573.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5314..6885
                     /gene="leuA"
                     /locus_tag="OO572_000454"
     CDS             5314..6885
                     /gene="leuA"
                     /locus_tag="OO572_000454"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6659574.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6885..7976
                     /gene="leuB"
                     /locus_tag="OO572_000455"
     CDS             6885..7976
                     /gene="leuB"
                     /locus_tag="OO572_000455"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6659575.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            7979..9379
                     /gene="leuC"
                     /locus_tag="OO572_000456"
     CDS             7979..9379
                     /gene="leuC"
                     /locus_tag="OO572_000456"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6659576.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9390..9995
                     /gene="leuD"
                     /locus_tag="OO572_000457"
     CDS             9390..9995
                     /gene="leuD"
                     /locus_tag="OO572_000457"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6659577.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10044..11222)
                     /gene="setA"
                     /locus_tag="OO572_000458"
     CDS             complement(10044..11222)
                     /gene="setA"
                     /locus_tag="OO572_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6659578.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11324..11455)
                     /gene="sgrT"
                     /locus_tag="OO572_000459"
     CDS             complement(11324..11455)
                     /gene="sgrT"
                     /locus_tag="OO572_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6659579.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11544..13199
                     /gene="sgrR"
                     /locus_tag="OO572_000460"
     CDS             11544..13199
                     /gene="sgrR"
                     /locus_tag="OO572_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6659580.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13233..13332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13363..14346
                     /gene="thiB"
                     /locus_tag="OO572_000461"
     CDS             13363..14346
                     /gene="thiB"
                     /locus_tag="OO572_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6659581.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14322..15932
                     /gene="thiP"
                     /locus_tag="OO572_000462"
     CDS             14322..15932
                     /gene="thiP"
                     /locus_tag="OO572_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6659582.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15916..16614
                     /gene="thiQ"
                     /locus_tag="OO572_000463"
     CDS             15916..16614
                     /gene="thiQ"
                     /locus_tag="OO572_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6659583.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16728..17492)
                     /locus_tag="OO572_000464"
     CDS             complement(16728..17492)
                     /locus_tag="OO572_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6659584.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17578..18456)
                     /gene="araC"
                     /locus_tag="OO572_000465"
     CDS             complement(17578..18456)
                     /gene="araC"
                     /locus_tag="OO572_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6659585.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18795..20495
                     /gene="araB"
                     /locus_tag="OO572_000466"
     CDS             18795..20495
                     /gene="araB"
                     /locus_tag="OO572_000466"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6659586.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20506..22008
                     /gene="araA"
                     /locus_tag="OO572_000467"
     CDS             20506..22008
                     /gene="araA"
                     /locus_tag="OO572_000467"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6659587.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22208..22903
                     /gene="araD"
                     /locus_tag="OO572_000468"
     CDS             22208..22903
                     /gene="araD"
                     /locus_tag="OO572_000468"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6659588.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            22978..25329
                     /gene="polB"
                     /locus_tag="OO572_000469"
     CDS             22978..25329
                     /gene="polB"
                     /locus_tag="OO572_000469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6659589.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25494..28400
                     /gene="rapA"
                     /locus_tag="OO572_000470"
     CDS             25494..28400
                     /gene="rapA"
                     /locus_tag="OO572_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6659590.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28412..29071
                     /gene="rluA"
                     /locus_tag="OO572_000471"
     CDS             28412..29071
                     /gene="rluA"
                     /locus_tag="OO572_000471"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6659591.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29188..30003)
                     /gene="djlA"
                     /locus_tag="OO572_000472"
     CDS             complement(29188..30003)
                     /gene="djlA"
                     /locus_tag="OO572_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6659592.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30258..32612
                     /gene="lptD"
                     /locus_tag="OO572_000473"
     CDS             30258..32612
                     /gene="lptD"
                     /locus_tag="OO572_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6659593.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32665..33951
                     /gene="surA"
                     /locus_tag="OO572_000474"
     CDS             32665..33951
                     /gene="surA"
                     /locus_tag="OO572_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6659594.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            33951..34940
                     /gene="pdxA"
                     /locus_tag="OO572_000475"
     CDS             33951..34940
                     /gene="pdxA"
                     /locus_tag="OO572_000475"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6659595.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34937..35758
                     /gene="rsmA"
                     /locus_tag="OO572_000476"
     CDS             34937..35758
                     /gene="rsmA"
                     /locus_tag="OO572_000476"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6659596.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35761..36138
                     /gene="apaG"
                     /locus_tag="OO572_000477"
     CDS             35761..36138
                     /gene="apaG"
                     /locus_tag="OO572_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6659597.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36145..36987
                     /gene="apaH"
                     /locus_tag="OO572_000478"
     CDS             36145..36987
                     /gene="apaH"
                     /locus_tag="OO572_000478"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6659598.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37065..37544)
                     /gene="folA"
                     /locus_tag="OO572_000479"
     CDS             complement(37065..37544)
                     /gene="folA"
                     /locus_tag="OO572_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6659599.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37735..39597)
                     /gene="kefC"
                     /locus_tag="OO572_000480"
     CDS             complement(37735..39597)
                     /gene="kefC"
                     /locus_tag="OO572_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6659600.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39590..40120)
                     /gene="kefF"
                     /locus_tag="OO572_000481"
     CDS             complement(39590..40120)
                     /gene="kefF"
                     /locus_tag="OO572_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6659601.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40168..41562)
                     /locus_tag="OO572_000482"
     CDS             complement(40168..41562)
                     /locus_tag="OO572_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6659602.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41621..41908)
                     /gene="fixX"
                     /locus_tag="OO572_000483"
     CDS             complement(41621..41908)
                     /gene="fixX"
                     /locus_tag="OO572_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6659603.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41905..43191)
                     /locus_tag="OO572_000484"
     CDS             complement(41905..43191)
                     /locus_tag="OO572_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6659604.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43242..44183)
                     /locus_tag="OO572_000485"
                     /pseudo
     CDS             complement(43242..44183)
                     /locus_tag="OO572_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44198..44968)
                     /locus_tag="OO572_000486"
     CDS             complement(44198..44968)
                     /locus_tag="OO572_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6659605.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45324..46838
                     /gene="caiT"
                     /locus_tag="OO572_000487"
     CDS             45324..46838
                     /gene="caiT"
                     /locus_tag="OO572_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6659606.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46869..48011
                     /gene="caiA"
                     /locus_tag="OO572_000488"
     CDS             46869..48011
                     /gene="caiA"
                     /locus_tag="OO572_000488"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6659607.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48140..49357
                     /gene="caiB"
                     /locus_tag="OO572_000489"
     CDS             48140..49357
                     /gene="caiB"
                     /locus_tag="OO572_000489"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6659608.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49431..50984
                     /gene="caiC"
                     /locus_tag="OO572_000490"
     CDS             49431..50984
                     /gene="caiC"
                     /locus_tag="OO572_000490"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6659609.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51093..51878
                     /gene="caiD"
                     /locus_tag="OO572_000491"
     CDS             51093..51878
                     /gene="caiD"
                     /locus_tag="OO572_000491"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6659610.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51884..52474
                     /gene="caiE"
                     /locus_tag="OO572_000492"
     CDS             51884..52474
                     /gene="caiE"
                     /locus_tag="OO572_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6659611.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52560..52955)
                     /gene="caiF"
                     /locus_tag="OO572_000493"
     CDS             complement(52560..52955)
                     /gene="caiF"
                     /locus_tag="OO572_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6659612.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53217..56438)
                     /gene="carB"
                     /locus_tag="OO572_000494"
     CDS             complement(53217..56438)
                     /gene="carB"
                     /locus_tag="OO572_000494"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6659613.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56456..57604)
                     /gene="carA"
                     /locus_tag="OO572_000495"
     CDS             complement(56456..57604)
                     /gene="carA"
                     /locus_tag="OO572_000495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6659614.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58060..58881)
                     /gene="dapB"
                     /locus_tag="OO572_000496"
     CDS             complement(58060..58881)
                     /gene="dapB"
                     /locus_tag="OO572_000496"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6659615.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59048..59962)
                     /gene="rihC"
                     /locus_tag="OO572_000497"
     CDS             complement(59048..59962)
                     /gene="rihC"
                     /locus_tag="OO572_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6659616.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60028..60978)
                     /gene="ispH"
                     /locus_tag="OO572_000498"
                     /gene_synonym="lytB"
     CDS             complement(60028..60978)
                     /gene="ispH"
                     /locus_tag="OO572_000498"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6659617.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(60980..61429)
                     /gene="fkpB"
                     /locus_tag="OO572_000499"
     CDS             complement(60980..61429)
                     /gene="fkpB"
                     /locus_tag="OO572_000499"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6659618.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61516..62010)
                     /gene="lspA"
                     /locus_tag="OO572_000500"
     CDS             complement(61516..62010)
                     /gene="lspA"
                     /locus_tag="OO572_000500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6659619.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62010..>63580)
                     /locus_tag="OO572_000501"
     CDS             complement(62010..>63580)
                     /locus_tag="OO572_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCR6659620.1"
                     /translation="ATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQARIESMVANRP
                     DWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAWWDLDAKEILG
                     DEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQHRGWFMSSLMI
                     STAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADILRLWVASTDY
                     TGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMVVLDRWAVGCA
                     KAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKADSVARRSCQT
                     ALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGLADSEAMNDAF
                     WDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALGDELRFVLLTS
                     GATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKVAEHAEICGRC
                     VSNVAGDGEKRKFA"
BASE COUNT        15404 a        17316 c        16407 g        14453 t
ORIGIN      
        1 ggtggcaatt gccaggtcag aaggcagttt gccgtcgcga cgcagcgtga catccatcac
       61 tccttgcaac aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag
      121 ccatttttcg aataactggg cagccgcctc ccgctcatag ctgttggcat acaggaaatg
      181 cacttcgcgc ggatcatctt tccaagcagt acggaaacct tgttcacgca ggaagctgac
      241 agaaagctcc ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc
      301 ttccgccagc atttcggcat catcctgatc ggcaccaacc acgctggtga aatgttcacg
      361 atcgagggcg cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa
      421 aggatgctca ggtggcaacg acgtcgaaac aataatggca tcaacctgac gctgtaaaag
      481 gtgctcaatg cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa tcagcagttg
      541 ataaccccgt tgccgcgcct ggcgttcaag atagttagcg atgcgggtat agctggtgtt
      601 ctccagatcg gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc
      661 cacggcgttc gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt
      721 gtcgctcaca cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga
      781 cactcccgcc agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa
      841 aaaccatagc cattgtactg gtatgggtta aattcattaa catctaagcg cagaaaatta
      901 actgcggcaa ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga
      961 catgttgggc tgcaaattgc gcactgagat cattctcaac gcattatttt atcgccgcgc
     1021 gaaagtccga ccacaccaga gcgagcaact tccacaattt tcgccacatc gcgaatcgat
     1081 gctaaaaatg catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggg
     1141 gtgacatcga taatttgccc acggaatatt tccgtattac gtttcacttc gtcacgcccg
     1201 taaccgctgg cctgaatttt caccagcatg atttcccgct caacatgcgc gccctgcccc
     1261 aactcactca cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ctgctcaagt
     1321 actttttcat cgcccacggt ctggatggtc atacgcgata atgtcggatc gtcggttggc
     1381 gcaacggtca ggctttcaat gttgtagcct cgctgggaaa aaaggccaat cacgcgggat
     1441 aacgcgcctg attcattttc gagtaagact gataatatcc ggcgcataat caggttctct
     1501 ccgttttgct taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt
     1561 gctcactgcc atcgacggta acatcaacaa acaccaggcg attattgcgc acctgttcca
     1621 gcgcctcgct aagtttgctt tccagctcat gcggatgaga gatctggatc ccgacgtgac
     1681 cataggcttc cgccagacgg acgaaatcgg gtagtgattg catataagat tgtgaatgac
     1741 ggccggaata gatcatgtcc tgccactgtt tcaccatccc cagatagcgg ttattgagat
     1801 tcaccaccag tacgggcaac tcgtattgca acgcggtaga cagttcctgg atgttcatct
     1861 gaatactgcc gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc
     1921 ccagtgccgc aggtaaacca aaacccatcg tgccgagacc gccggaattg atccagcgac
     1981 gcggtttgtc gaatggataa taaagcgcag caaacatctg gtgctgcccg acatcggacg
     2041 tcacgtaagc gtccccatta gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa
     2101 tcttttcact gtgagtgtca tatttcaggc actgaagagc gcgccactgt tcaatctgct
     2161 gccaccagtc gcggatctca tccagcggtt gatgggcgga ttcttgcgac aggagttcaa
     2221 gcatttgttc gaggacctgg cgagcatccc ccacaatcgg gatatccgca gtcacggttt
     2281 tagaaatgga agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg
     2341 ccagattgtt cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc acatccgcgt
     2401 tatgcatcgt catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac
     2461 gatgcgttgc cggaaacgcc cccattccca tcaatgagga aacaacgggc agattcaacg
     2521 cctccaccgt ttctttcaac tgctgatggc agcccgccat gattgcccca ccgcctacgt
     2581 agacaaccgg tttttttgcc gctaccagcg tttgcagagc acgcttaatt tgccctttat
     2641 gtccggtagt agtgggattg taagaacgca tactgaccga ctcagggcag acatagggta
     2701 atttgttcgc cggattaaga atatctttcg gtaaatcaac gaccactggt cctgggcgac
     2761 cacttgccgc cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa
     2821 ccagaaaact gtgtttaacc accggtcgcg aaatccccac catgtcgcac tcctgaaagg
     2881 catcgtaacc tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat
     2941 ccatataagc ggtggcgatg ccggtaatcg cattggtcgc ccttggaccc gacgttacca
     3001 gcacgacgcc gacttccccg gtcgcacgcg ccaggccatc ggccatatgc accgccgcct
     3061 gctcatgacg aactaataca tgatcaatac cacccacggt atgcaatgca tcgtaaatat
     3121 caaggactgc gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc
     3181 ggacgaccat ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa
     3241 aatgtgtaaa acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa
     3301 acgcagggta ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt
     3361 acgaaggatt aaattgataa taaatcggct gaatcccaca acttacagca atttctctat
     3421 ttttttaatt aatcctaaaa tagagagtcc acacattcag aataaaattc tacattcaat
     3481 aaaaaatgca gaataaacca gacattcatg tctggcctat tctgcattca gttagcgttt
     3541 gcaaattgag actaattgtt cttccatcca ctgatggcct ttatcgcgcc cggcagcttc
     3601 atgccaagag agataacagg ttctgctgtt ttgttttaac ggcagcggta atacctgtaa
     3661 ttctaaggat tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt
     3721 ttgcgacacc acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg
     3781 cttatctacc gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc
     3841 cgcatgttgt tcgttataaa catcatgttt cagtaacggg cctttaattg ttggatgatt
     3901 tttgctggct accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg
     3961 atggaagtct tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt
     4021 gttctgattt aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg
     4081 attataaatc tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac
     4141 acgttcactg ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa
     4201 tgcctggcga actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc
     4261 ataacgaaca aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac
     4321 cgcaggttgc gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac
     4381 ggcatcgaaa acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc
     4441 cgccgtctct gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa
     4501 ctccctttcc cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat
     4561 ataaaataag aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat
     4621 aaaaacagaa tccattgccg tggaatgagt catttaccac agcataataa tccataatgt
     4681 tttgttgcga aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga
     4741 ttaaatgtgc ttatttaata attaatttat atatttaatg cattaattct taacattaat
     4801 tgttcaataa tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac
     4861 aaatcatcag gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag
     4921 atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa
     4981 ttaatatcgc ttactttaac caccgtagca caattagcta attttacgga tgcagaactc
     5041 acgctggcgg gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc
     5101 taaaagcata tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg
     5161 tctactacta ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta
     5221 agccagcacg cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag
     5281 cgaggcgctc taaaagagac aaggacccaa accatgagcc agcaagtcat tattttcgat
     5341 accacattgc gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg
     5401 caaattgcgc tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc
     5461 tcttcgccgg gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc
     5521 gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa
     5581 gtcgccgaag ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc
     5641 aagctgcgca gcaccctaga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc
     5701 cgtaattaca ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc
     5761 gatctggcgc gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg
     5821 gacaccgtgg gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa
     5881 cgcgtaccta acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg
     5941 gcggtcggca actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg
     6001 aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa
     6061 gttcgtaagg atattctcaa cgtccacacc gccattaatc accaagagat atggcgcacc
     6121 agccagttag ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc
     6181 agcggcgcat tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa
     6241 aactacgaaa tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc
     6301 tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt
     6361 gaatataatt tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag
     6421 gtgtttgatt acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag
     6481 catttccgtc tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc
     6541 gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc
     6601 gatgccgtct atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac
     6661 agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcaccaac
     6721 tacaacggtc gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc
     6781 aaagccatgg tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg
     6841 caacgcaaaa ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc
     6901 atattgccgt attgccggga gacggtattg gtccggaagt gatgacccag gcgctgaaag
     6961 tgctgaatgc cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag
     7021 gcggcgcagc cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg
     7081 agcaagccga tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac
     7141 cagaccagca accagaacgc ggcgcgctgc tgccgctgcg taagcacttc aaattattca
     7201 gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag
     7261 acattgccgc taacggcttc gacatccagt gcgtgcgcga actgaccggc ggcatctatt
     7321 tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg
     7381 tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac
     7441 gtcgccacaa agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc
     7501 gtgaaatcgt caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca
     7561 tcgacaacgc caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct
     7621 ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggca
     7681 tgttgccttc cgccagcctg aacgagcaag gttttggact gtatgaaccg gcgggcggct
     7741 cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat tgcacaaatc ctgtcgctgg
     7801 cactgctgct gcgttacagc ctggatgccg atgatgcggc ttccgccatt gaacgcgcca
     7861 ttaaccgtgc attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg
     7921 ttagtaccga tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat
     7981 ggctaaaacg ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga
     8041 aaccccgctg ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt
     8101 cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat
     8161 ggatcacaac gtctctaccc agaccaaaga cattaatgcc tgtggtgaaa tggcgcgcat
     8221 ccagatgcag gagctgatta aaaactgcaa agaatttggc gtcgagctgt atgacctgaa
     8281 tcacccgtat caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg
     8341 gatgaccatt gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc
     8401 ctttggtatc ggcacttccg aagttgaaca cgtactggca acacaaaccc tgaaacaggg
     8461 ccgtgcgaag accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa
     8521 agatatcgtg ctggcaatta tcggtaaaac tggcagcgca ggcggcaccg ggcatgtggt
     8581 ggagttttgc ggcgaagcaa ttcgtgattt aagcatggaa ggtcgtatga ccctgtgcaa
     8641 tatggcgatc gaaatgggcg caaaagccgc tctggttgct cctgacgaaa ccacctttaa
     8701 ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta
     8761 ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc
     8821 agaagaaatt tcaccgcagg tcacctgggg caccaatccc ggccaggtga tttctgtgaa
     8881 cgacaatatt cccgatccgg cttcgtttgc cgatccggtt gaacgtgcgt cggcagaaaa
     8941 agcgctggcc tatatggggc tgaaaccggg tattccgctg accgaagtgg ctatcgacaa
     9001 agtgtttatc ggttcctgta ccaactctcg cattgaagat ttacgcgcgg cagcggagat
     9061 cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc
     9121 ggtaaaagcc caggcggaag cggaaggtct ggataaaata tttattgaag ccggttttga
     9181 atggcgcttg cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg
     9241 cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg
     9301 cacgcatctg gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga
     9361 cattcgcaac attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc
     9421 tggtggttcc gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt
     9481 tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg
     9541 atgaaaaagg ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg
     9601 cttccatttt gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct
     9661 gggcattgac cgactacggt tttaaagtgg tgattgcgcc gagttttgct gacatcttct
     9721 acggcaatag ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg
     9781 aactgtttgc gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc
     9841 aagaggtgaa agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact
     9901 gcatgatgaa tggtctggac agcatcggcc tgaccctgca gcacgacgac gctattgcgt
     9961 cttatgaagc aaaacaacct gcgtttatga attagacccc ttgcccggtc aaatgaccgg
    10021 gctttccgct atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg
    10081 acaacagaaa taatcgcaat tacccagtag acagcaaagt gcccccaact ttgtgcaatt
    10141 gctccctgaa taacaccagc cagaattacc ccggtagaga tagtgttggt aaataaggtg
    10201 gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc atagcatccc aatacccgca
    10261 acaatgccga taaatacagc gttaaaaagt tgcagcgtca tcaacgccat acggctatga
    10321 aagaaaatca atccagtgta aaacagcact cctgccacca ctgcaatgac catcattcgc
    10381 cgcttaccat aacgtttgac atagtagcca gccagaatca ttgctggtat ttccagtcca
    10441 gctgccgtcc ccatcaggaa acccgccagt ttgtctggca atcctaaatc gctactgatc
    10501 cacaatggca tatcaataat gtacatagtg ttgcaggtcc acattaacgt cgaggcgaca
    10561 aataacatcc gtacgttact atcctgccag tcaccttgca ttgataaagc attttccgac
    10621 ggcagttcta cccgcgccac agacagaagc ataaatgcaa tcaataccag actgagtgtg
    10681 aatatcccgg cggcaatcga aaacatcact gtaaagccgt aattcaacgc cagcataaag
    10741 gccagcggtg gaccgattac ccatgccaga gaaagctgcg cacgcatcac cgagctaaac
    10801 atcaccactt ctcgcgccga gttatccgcg tattcccgcg ccagagcaaa taactgtggc
    10861 attgccgtat tggccagaga tgccagaagc acaccacagg tgataagcgt cagataatga
    10921 cgattaaatg caaacagtag cgcattgcca atagccatca aacagcaaaa tataatcagt
    10981 tttcgccgat cgccctgact gtcagaacgt tttgccaacc agaggcttac gccgatccca
    11041 gcaatagcat tcaccgtata aaagagtccg atccagaaag gttgcgcgcc aacctcacga
    11101 ctcagaaata agctcaatgt aggagcctgt agcgccccgg ccacccccat cataaaagcg
    11161 accagcataa atgccgcgta aacaccgttc atacggcgag ccatcgtcat tatccagatc
    11221 atacgttccc tttttagcgc ggcgagaata aaaaaaacca gcaggtataa tctgctggcg
    11281 ggtgatttta caccaatact cagtcacaca tgatgcaggc aagtcaactt tcagaattgc
    11341 ggtcatccca ctgcatcagt ccttcaagca tggttaatcg ttgtgggacg cttaaccaac
    11401 gcaaccagca caacttcgct gtcgcggtaa aatagtgctg ataaaactga cgcatggggc
    11461 acctccttgc ttcatcgtta ggaattattg acttaatata gggaaaataa aattgctgtc
    11521 ttttgcgcag gagttcccct tttatgccat ctgctcgtct gcaacaacag ttcatccgcc
    11581 tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct caacgaactg gcagcgttat
    11641 tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac catgcaggat cgcggctggc
    11701 tgacgtggga agcggaagtc gggcgcggta aacgctcgcg tctgacattc ctctataccg
    11761 ggctggcgct tcagcaacag cgggcggaag acctgctgga gcaggatcgt atcgaccaac
    11821 tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat gctggtttct catctgggcc
    11881 gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta ctatcgtccg ttgcgtaatc
    11941 tgctacctgg cagcgcattg cgccgttccg aaacccatat tgcccggcaa atcttcagtt
    12001 cgctaacgcg cataaatgag gaaaatgggg aactggaagc agacatcgcc caccactggc
    12061 agcaaatatc accgcttcac tggcgtttct ttttgcgtcc aggagtccat tttcatcatg
    12121 gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa acgaatcaat acgctgccgc
    12181 tctattcgca tattacggac attgtgtcgc cgacgccctg gacgctggat atccatctca
    12241 cgcagccgga ccgctggttg ccgttactgc tggggcaagt tccggcgatg atcctgccgt
    12301 gcgaatggga aaccctcagt aactttgcca gccatcctat tggcaccggt ccgtatgcgg
    12361 tgattcgcaa cagcaccaat caactgaaaa ttcaggcatt cgatgacttc ttcggttacc
    12421 gggcattaat cgacgaagtt aacgtctggg ttctgccgga aattgccgac gagccagccg
    12481 gtggtctgat gctaaaaggt ccacagggcg agaaaaaaga gattgaaagc cgcctggagg
    12541 aaggttgcta ctatttactg ttcgacagcc gcacccatcg cggggcgaat cagcaagtca
    12601 gggactgggt aagctatgtg ctttctccaa ctaatctggt ctatttcgct gaggaacagt
    12661 accagcaact gtggttcccg gcttatggac tgctcccccg ttggcaccat gcccgcacca
    12721 taaagagcga aaaaccggct ggcctggaaa gcctcaccct gaccttttat caggatcaca
    12781 gtgagcatcg ggtgattgcc gggatcatgc agcagattct ggcaagtcac caggtcacgc
    12841 tggaaatcaa agagatcagc tacgatcagt ggcatgaagg agagatcgag agcgatatct
    12901 ggcttaatag cgccaacttt acgctgccgc tggatttttc gctgttcgcg cacttgtgcg
    12961 aggtgccgct gctccaacac tgtattccga tcgactggca agccgacgcc gcccgctggc
    13021 gcaatggcga aatgaacctg gcgaactggt gccagcaact ggtcgccagt aaagcaatgg
    13081 tgccgcttat ccaccactgg ctgatcattc agggacaacg cagtatgcgc ggcctgcgta
    13141 tgaataccct cggctggttc gattttaaat cagcgtggtt tgcgccaccg gatccatgat
    13201 tgctggtaga taacaaaatc actacactaa cgccgttctc aacggggtgc cacgcaaacg
    13261 cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa gggatttgag
    13321 gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt
    13381 cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc
    13441 tacgattcct tcgccgccga ctgggggcct ggtccaaaga tcaaaaaagc ttttgaagcc
    13501 aactgtaact gcgaactgaa actggtggcg ctggaagatg gcgtttcgct tctcaaccgt
    13561 ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc tggggctgga taacaatctg
    13621 ttagacgccg ccagtaaaac cggactattt gccaaaagcg gtgtggcagc ggatgccgtt
    13681 aacgttcccg gcggctggaa taatgacact ttcgtcccgt ttgattacgg ctatttcgcc
    13741 ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa gcctgaaaga attggttgag
    13801 agcgatcaga actggcgggt gatttatcag gatccgcgta ccagcacgcc ggggctgggt
    13861 ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc cacaagcctg gcaaaaactg
    13921 gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag cctacggcct gtttttaaaa
    13981 ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat tctcgaagag
    14041 aaaaaagata actacgccgc cgcgaacttt agcgaaggtc actatctgca ggtggaagtc
    14101 gccgcccgca ccgctgccag caagcagccg gagctggcgc aaaaattcct ccagtttatg
    14161 gtttctccgg cttttcagaa tgcgatccca accggtaact ggatgtatcc ggtggcaaat
    14221 gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg ccaccacgct ggagttcaca
    14281 ccagctgaag tggcggcaca acgtcaggca tggattagcg aatggcaacg cgccgtcagc
    14341 cgttaattcc cggctggtta attccaggtg taagcgccgc cacgctggtg gtagcggttg
    14401 cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca gggtaactgg gtggcagtct
    14461 ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg tttctctcgg
    14521 cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc
    14581 cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg gtgctagttg
    14641 ctgttttcgg cattcttagc gtctatggtc gtcagggctg gctggcatcg ctctgccaat
    14701 cgctcggtct ggagtggacc ttttcgccct acggcctgca aggcattttg ctggcgcacg
    14761 tattttttaa tctgccgatg gcgagccgct tattactcca ggcactggaa aacattcccg
    14821 gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg
    14881 tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc cgcgcttatc tttatgctct
    14941 gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg tccgcaggca accactatcg
    15001 agctggctat ttatcaggcg ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg
    15061 cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg
    15121 ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga tccggacgat cgtctgcata
    15181 gccgcatttg cgacacggtg ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc
    15241 tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga agtgctggca caaccggtgc
    15301 tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag
    15361 tgctgaccat gatgctgcta tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg
    15421 cgggtcaggc gctggagatg agcggcatgt tgatcctcgc catgccgggg attgtgctgg
    15481 ctaccggctt ctttttactg ctcaacaaca ctatcggcct gccacaatct gctgacggca
    15541 ttgtgatttt caccaatgcg ttaatggcga tcccttatgc gctgaaagta ctggaaaacc
    15601 cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca gtcgctgggc attgaaggct
    15661 ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg tccactggcg caggcgctgg
    15721 cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg
    15781 atttccgtac cctgccgttt tatctctacc agcaaattgg ctcctatcgc agccaggacg
    15841 gcgcggtcac cgcgttaatt ctgctactac tctgttttct gctgtttacc gtgattgaaa
    15901 aactaccggg gcgaaatgtt aaaactgact gatatcacct ggctttatca ccatttgccg
    15961 atgcgtttta gcttaacggt agaacgcggc gagcaggtgg caatcctcgg gccaagcggc
    16021 gcgggtaaaa gtaccctgct gaatttgatt gccggttttc tgacgccagc cagcggttcg
    16081 ctgactatcg atggtgttga tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg
    16141 ctgtttcagg agaacaacct gttcagccat ctgacggtcg cgcagaacat cgggctgggg
    16201 ctaaatccgg ggttgaaact gaacgcggca cagcaggaga aaatgcacgc gattgcccgt
    16261 cagatgggga ttgataattt aatggcgcgg ttaccgggcg agctttccgg cggtcagcga
    16321 cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa
    16381 ccgttctctg cgctcgatcc ggcgttacgt caggagatgc tgacgctggt gagcacgagc
    16441 tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg
    16501 atcgccacac gctcggtggt ggtcgccgac gggcgcatcg cctggcaggg taagactgaa
    16561 gaattgttga gcggtaaggc gagtgcttcg gcaatattgg ggattacggg ttagtatgca
    16621 ctgtcagatg cggcgtgaac accttatccg acctacggac agtgcgtttt gtaggcctga
    16681 taagacgcgg tcagcgtcgc atgaggcaac ccgcacaaga caatccccta aaccccaacc
    16741 actttacgca aaatatcgat atacaccggc atcaacgggt ggcgaattaa caccaccagc
    16801 gccaccacgc cgatggcaga aatcaacggc gtcagccaca acaaacggcc gcgcgacaaa
    16861 taatgactta aacgatcggt tgctttaccg ctgcgccata accgccagca cagccagcca
    16921 ccaacccaca aaaataccgc cgtcgccagc agcaaccatt taaactcgcc gctctgcatt
    16981 ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag gcaggaagta aaacggtggc
    17041 cacagcaggc agccgataat attcggcgta atgaattttg ccaccggcag atccagcatt
    17101 cccgccacca ttggcaccag cggacgcgtc gggccaacaa agcgaccgac cagaatggtg
    17161 aacatgctgt gttgatgcaa cgcatgttca gttttatcga gtaatgcttt gtttttcttc
    17221 agaaatgacc agcgatgcaa cggcttttta aaacgccagc ccagccagaa ggagatccag
    17281 tcgcccagca gacaaccgac aatccctgcc agccaagcgt gccagaaact taactcgccg
    17341 ctgccaatca gcgctcccag ccccgccatc agcaccgtac cgggtagaat caaaccgacc
    17401 agcgccagcg actccagaaa ggccaccaac accaccgcca tcaatgaata cacggtagat
    17461 tgggtaataa agtgttccag caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg
    17521 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
    17581 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
    17641 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
    17701 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
    17761 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
    17821 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
    17881 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acacgattat ccatcggtgg
    17941 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagattcat
    18001 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
    18061 gtcgctgaaa tacggctggt gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat
    18121 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
    18181 ataccattcg cgagcctccg gatgacaacc gtagtgatga atctctcctg gcgggaacag
    18241 caaaatatca cccggtcggc agacaaattc tcgtccctga tttttcacca ccccctgacc
    18301 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
    18361 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
    18421 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
    18481 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct
    18541 cttctcgcta acccaaccgg taaccccgct tattaaaagc attctgtaac aaagcaggac
    18601 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat
    18661 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
    18721 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg
    18781 gatggagtga aacgatggcg attgcaattg gcctcgattt tggcagtgat tctgtgcgag
    18841 ctttggcggt ggactgcgcc agcggtgaag agatcgccac cagcgtagag tggtatcccc
    18901 gttggcaaaa agggcaattt tgtgatgccc cgaataacca gttccgtcat catccgcgtg
    18961 actacattga gtcaatggaa gcggcactga aaaccgtgct tgcagagctt agcgtcgaac
    19021 agcgcgcagc tgtggtcggg attggcgttg acagtaccgg ctcgacgccc gcaccgattg
    19081 atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt
    19141 tcgtattgtg gaaagaccac actgcggttg aagaagcgga agagattacc cgtttgtgcc
    19201 acgcgccggg caacgttgac tactcccgct atattggcgg tatttattcc agcgaatggt
    19261 tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc cgtggcgcaa tctgccgcat
    19321 cgtggattga gctgtgcgac tgggtgccag ctctgctttc cggtaccacc cgcccgcagg
    19381 atattcgtcg cggacgttgc agcgccgggc ataaatctct gtggcacgaa agctggggcg
    19441 gcttgccgcc agccagtttc tttgatgagc tggacccgat cctcaatcgc catttgcctt
    19501 ccccgctgtt cactgacacc tggactgccg atattccggt gggcacctta tgcccggaat
    19561 gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc
    19621 atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt
    19681 ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca gttaaaggta
    19741 tttgcggtca ggttgatggc agcgtggtgc ctggatttat cggtctggaa gcaggccaat
    19801 cggcgtttgg tgatatctac gcctggtttg gtcgcgtact cggctggccg ctggaacagc
    19861 ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc cagccagaaa caactgcttc
    19921 cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg
    19981 actggtttaa cggtcgtcgc acgccaaacg ctaaccaacg cctgaaaggg gtgattaccg
    20041 atcttaacct cgctaccgac actccgctgc tgttcggcgg tttgattgct gccaccgcct
    20101 ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg gatcgccgtc aataacgtga
    20161 tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc
    20221 tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt gcggcgattt
    20281 ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa aaaatggcca
    20341 gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc acaacgcttt gaacagcttt
    20401 atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ctatcttcca acttccgccc
    20461 cggcacaggc tgcccaggcc gttgcgactc tataaggaca cgataatggc gatttttgat
    20521 aattatgaag tgtggtttgt cattggcagc cagcatctgt atggcccgga aaccctgcgt
    20581 caggtcaccc aacatgccga gcacgttgtt aatgcgctga atacggaagc gaaactgccc
    20641 tacaaactgg tgttgaaacc gctgggcacc acgccggatg aaatcaccgc tatttgccgc
    20701 gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt ggctgcacac cttctccccg
    20761 gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac cgttgctgca attccacacc
    20821 cagttcaacg cggcgctgcc gtgggacagt atcgatatgg actttatgaa cctgaaccag
    20881 actgcacatg gcggtcgcga gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc
    20941 gtcgttaccg gtcactggca ggataaacaa gcccatgagc gtatcggctc ctggatgcgt
    21001 caggcggtct ctaaacagga tacccgtcat ctgaaagtct gccgatttgg cgataacatg
    21061 cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac agatcaagtt cggtttctcc
    21121 gtcaatacct gggcggttgg ctatctggtg caggtggtga actccatcag cgacggcgat
    21181 gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacgcctgc cacacaaatc
    21241 cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt
    21301 ttcctggaac aaggtggctt ccacgcgttc accaccacct ttgaagattt gcacggtctg
    21361 aaacagcttc caggtctgcc cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc
    21421 gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga aggtgatgtc aaccggtctg
    21481 cagggcggca cctcctttat ggaggactac acctatcact tcgagaaagg taatgacctg
    21541 gtgctcggct cccatatgct ggaagtctgc ccgtcgattg ccgtagaaga gaaaccgatc
    21601 ctcgacgttc agcatctcgg tattggtggt aaggacgatc ctgcccgact gatcttcaat
    21661 acccaaaccg gtccagcgat tgtcgccagc ctgattgatc tcggcgatcg ttaccgtctg
    21721 ctggttaact gtatcgacac ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg
    21781 aatgcactgt ggaaagcgca accggatctg ccaactgctt ccgaagcgtg gatcctcgct
    21841 ggtggcgcgc accataccgt cttcagccat gcgctgaacc tcaacgatat gcgccagttc
    21901 gccgagatgc acgacattga aatcacggtg attgataacg acacccgcct gccagcgttt
    21961 aaagacgcac tgcgctggaa cgaagtgtat tacggatttc gtcgctaagt agccacatca
    22021 ggtatgtaac gcctgatgcg acgctgacgc gtcttatctg gcctacacgc tgcgattttg
    22081 taggccggat aagcaaagcg catccggcat tcaacgcctg atgcgacgct ggcgcgtctt
    22141 atcaggccta cgcgctgcga ttttgtagac cggataagcg aagcgcatcc ggcacgaagg
    22201 agtcaacatg ttaaaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa
    22261 acacaacctg gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt
    22321 ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg accgctgacg atatggtcgt
    22381 ggttagcatc gaaaccggtg aagtggttga aggtacgaaa aagccctcct ccgacacgcc
    22441 aactcaccgg ctgctctatc aggcattccc ctccattggc ggcattgtgc atacgcactc
    22501 gcgccacgcc accatctggg cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca
    22561 cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa
    22621 cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa acctttgaaa aacagggtat
    22681 cgatgcagcg caaatgcccg gcgttctggt ccattcccac ggcccgtttg catggggcaa
    22741 aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt atatggggat
    22801 attctgccgt cagttagcgc cgcagttacc ggatatgcag caaacgctgc tggataaaca
    22861 ctatctgcgt aagcatggcg cgaaggcata ttacgggcag taatgactgt ataaaaccac
    22921 agccaatcaa acgaaaccag gctatactca agcctggttt tttgatggat tttcagcgtg
    22981 gcgcaggcag gttttatctt aacccgacac tggcgggaca ccccgcaagg gacagaagtc
    23041 tccttctggc tggcgacgga caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc
    23101 gtggcgttta ttcacgccga tcaggttccc cgcgctcagc atattttgca gggtgaacaa
    23161 ggctttcgtc tgacaccgct ggcgttaaag gattttcacc gccagccggt gtatggcctt
    23221 tactgtcgcg cccatcgcca attgatgaat tacgaaaagc gtctgcgtga aggtggcgtt
    23281 accgtctacg aggccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc
    23341 tcaccggtgt gggtcgaggg tgatatgcac aatggcacta tcgttaatgc ccgtctgaaa
    23401 ccgcatcccg actatcgtcc gccgctcaag tgggtttcta tagatattga aaccacccgc
    23461 cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg
    23521 gggccggaga atggcgacgc ctcctcgctt gatttcgaac tggaatacgt cgccagccgc
    23581 ccgctgctgc tggaaaaact caacgcctgg tttgcaaacc acgatcccga tgtgatcatc
    23641 ggctggaacg tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt
    23701 cttccgctgc gtcttgggcg cgataatagc gagctggagt ggcgcgagca cggctttaaa
    23761 aacggcgtct tttttgccca ggctaaaggt cggctaatta tcgacggtat cgaggcgctg
    23821 aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ctgtcgctca ggagctatta
    23881 ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc
    23941 gccgaagata aacctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag
    24001 atcttccaca aaactgaaat catgccattt ttactcgaac gagcaacggt gaacggcctg
    24061 ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc atctctattt tccgcgaatg
    24121 catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc
    24181 ggctacgtga tggattcacg gccagggctt tatgattcag tgctggtgct ggactataaa
    24241 agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggttgaaggc
    24301 atggcgcaac ctgatccaga gcacagtacc gaaggttttc tcgatgcctg gttctcacgg
    24361 gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga taaagccaaa
    24421 cgtcagggta ataagccgct ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc
    24481 gtgctcggca ccaccgcctg ccgcttcttc gatccgcgcc tggcatcgtc gatcaccatg
    24541 cgtggtcatc agatcatgcg gcagaccaaa gcattgattg aagcgcaggg ctacgacatt
    24601 atctacggag ataccgactc gacttttgtc tggctaaaag gtgcacattc ggaagaagag
    24661 gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg cctggtgggc ggaaacgctg
    24721 caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaactcattt ctgccgtttt
    24781 cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa agcgctacgc cgggctgatt
    24841 caggagggcg acaagcagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg
    24901 acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg caacgagcca
    24961 tatcaggagt acgtccgcca aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg
    25021 ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct
    25081 catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa agcgcggtcg ccccttgcaa
    25141 tatcagaatc gcggcaccat taagtacgta tggaccacca acggcccgga gccgctggac
    25201 taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg
    25261 gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg
    25321 ctattttgag caaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg
    25381 ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa
    25441 gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt
    25501 ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg
    25561 tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt
    25621 acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca
    25681 gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct
    25741 atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg
    25801 atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta
    25861 tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc
    25921 cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc
    25981 atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga
    26041 aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg
    26101 tgctgattat cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt
    26161 tcaacctgcg ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt
    26221 acaacccgtt tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca
    26281 aacagcgctt ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc
    26341 atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg
    26401 cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa
    26461 gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg
    26521 ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg ctggcaggta
    26581 acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg
    26641 agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc
    26701 tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta
    26761 acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc
    26821 agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc
    26881 gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca
    26941 cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct
    27001 ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac
    27061 tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac
    27121 gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct
    27181 cagaaatcgg ttctgaagga cgtaactttc agttcgccag tcacatggtg atgtttgacc
    27241 tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg
    27301 cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc
    27361 gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt
    27421 acgatagcgt atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct
    27481 ttgacgatct gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac
    27541 agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg
    27601 cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt
    27661 tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt
    27721 ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct
    27781 ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc
    27841 tgatccgcaa cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac
    27901 tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg
    27961 aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta
    28021 tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc
    28081 gccagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc gtgcagcagg
    28141 atgttcacgc tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga
    28201 ttgatgcagc gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag
    28261 ctctgcgtgc agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc
    28321 gtcagcaggt aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga
    28381 tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca
    28441 ggaaccctgg ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag
    28501 cggtttgttg tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat
    28561 tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt
    28621 gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg
    28681 cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct
    28741 ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga
    28801 aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc
    28861 aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc
    28921 gctggggcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc
    28981 accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag
    29041 tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg
    29101 ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg
    29161 cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc
    29221 atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa
    29281 acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt
    29341 gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt
    29401 cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc
    29461 aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc
    29521 taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc
    29581 aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca
    29641 gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg
    29701 gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg
    29761 gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat
    29821 cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc
    29881 cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc
    29941 ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg
    30001 catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct
    30061 tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac
    30121 ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg
    30181 tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg
    30241 aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca
    30301 ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg
    30361 tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca
    30421 atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata
    30481 tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac
    30541 caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata
    30601 accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg
    30661 tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga
    30721 aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc
    30781 cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag
    30841 ttgctgagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct
    30901 atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt
    30961 acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata
    31021 tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg
    31081 aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag
    31141 ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact
    31201 ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca
    31261 gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg
    31321 caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca
    31381 agcagttcca ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag
    31441 acgttaatta ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag
    31501 tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga
    31561 ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg
    31621 caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg
    31681 acgaatccgt taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac
    31741 gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt
    31801 tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat
    31861 ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg
    31921 ctaaccaagt gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt
    31981 ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca
    32041 taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc
    32101 gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg
    32161 tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga
    32221 attaccatta cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg
    32281 ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg
    32341 atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact
    32401 ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga
    32461 agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta
    32521 acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt
    32581 cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt
    32641 gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc
    32701 atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc
    32761 aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac
    32821 gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa
    32881 cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc
    32941 gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat
    33001 cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc
    33061 cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc
    33121 ctgccacagg aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact
    33181 gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg
    33241 aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc
    33301 ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg
    33361 tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa
    33421 ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac
    33481 ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg
    33541 aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct
    33601 gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca
    33661 ggttctgcta accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc
    33721 ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca
    33781 ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg
    33841 cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc
    33901 tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa
    33961 cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc
    34021 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta
    34081 ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc
    34141 ctgcacaacc gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg
    34201 tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag
    34261 cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag
    34321 gcgtcattaa cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt
    34381 cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa
    34441 cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag
    34501 tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc
    34561 tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag
    34621 acaccattat tccggtgctc gacgagctgc gggcgcaggg gatgaaactc aacgggccgc
    34681 tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga
    34741 tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca
    34801 ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg
    34861 cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa
    34921 tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt
    34981 cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc
    35041 gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga ccgagccggt
    35101 cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct
    35161 gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt
    35221 taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc
    35281 ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga
    35341 catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa
    35401 agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga
    35461 agttccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt
    35521 ccctcatgca acgatgcctc acccggttaa agatattcgc gtgttgagcc gcatcaccac
    35581 cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt
    35641 cgaggtgtta accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc
    35701 gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg
    35761 atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct
    35821 tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga
    35881 gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc
    35941 gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac
    36001 cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa
    36061 atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt
    36121 cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt
    36181 tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc
    36241 tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg
    36301 aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg
    36361 gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg
    36421 ccggatgccg acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa
    36481 gagaaaaagc tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc
    36541 aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt
    36601 gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga
    36661 ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg
    36721 gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg
    36781 attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa
    36841 ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca
    36901 ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag
    36961 gatttgggcg aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg
    37021 cgaccggcgt cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga
    37081 atctcaaagc aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc
    37141 gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg
    37201 atatgcgtca gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg
    37261 ccaatcacca tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc
    37321 acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct
    37381 ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag
    37441 gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg
    37501 ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa
    37561 aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt
    37621 tgaggaaaat tttatattct gctggcgagt ccacgctctc cccctggact cgccgcatta
    37681 caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga
    37741 tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc
    37801 ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact
    37861 taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat
    37921 tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc
    37981 ttcatacggc cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc
    38041 aaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac
    38101 atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag
    38161 ttgcaggttg gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc
    38221 ggcagattcc agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt
    38281 acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga
    38341 gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg
    38401 ctgttcttca tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg
    38461 attgaggatc accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag
    38521 cgatttcacc cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa
    38581 ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac
    38641 ctgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag
    38701 caaaatgaca atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac
    38761 accgatgaaa aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc
    38821 atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga
    38881 caagccgacc tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact
    38941 aaacacttcc cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg
    39001 ccccagcaat accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc
    39061 gcccatcgtc gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat
    39121 tgccgcgata tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat
    39181 cagattgcgt tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat
    39241 accgatcaat tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag
    39301 gccaccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca
    39361 cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc
    39421 ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat
    39481 gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac
    39541 aatcagcgcc gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat
    39601 gggcctcctg ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt
    39661 cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt
    39721 agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt
    39781 caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg
    39841 ccgtgccgcc gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa
    39901 gaagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag
    39961 agagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag
    40021 agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg
    40081 aatgatgcgg atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt
    40141 acgcctttgt tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat
    40201 tgattcagaa aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat
    40261 attgctggtc tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa
    40321 caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt
    40381 gataaatatc ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat
    40441 cacgcccacg gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg
    40501 caaattaccc ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca
    40561 gatccccata tccgggatta gccccaaaac cgccaacgcc agcgtcatca tggcaaagct
    40621 gccaatcaac aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc
    40681 gagcataaag aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa
    40741 tcccaacgaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg
    40801 gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc
    40861 gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt
    40921 tatcatcatc tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc
    40981 tggcagttcg aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat
    41041 ccagcgccaa ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc
    41101 gccgacatac cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga
    41161 gaactcggtg atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag
    41221 tacccgcatc accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac
    41281 gccgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata
    41341 gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat
    41401 ccagtcagcg tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac
    41461 cagtacataa ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat
    41521 agaggagaat ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt
    41581 tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca
    41641 caccaaaggt gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc
    41701 gacaggtgcc gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct
    41761 tcttgtacag acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat
    41821 cggccttcac aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt
    41881 tgacgtccac attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat
    41941 cagattgatg aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg
    42001 cgcactgccg tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggttgcc
    42061 gctaaacatg cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg
    42121 cagcgggcca ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc
    42181 gcttttcatc gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc
    42241 cataccgcga atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa
    42301 tacgccgtca ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc
    42361 ggaatattcc accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa
    42421 atcttccagc atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac
    42481 cagccccagc gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc
    42541 ggtgggtgat cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc
    42601 ttcaattacc gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg
    42661 tttaacgcgt tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag
    42721 gagcaccgtt ttcgcttcaa tcacatcacc atcggcttct acaccgacga ctttgccatc
    42781 gcgctgtacg aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc
    42841 ctgctccatc aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatgg
    42901 cgaggtttcg tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc
    42961 gagtttttca tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat
    43021 gtgttccaga ctgtgggcat agagacgccc gccagtaacg ttcttggcac ctgcggaatt
    43081 gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga
    43141 accggcaagc cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc
    43201 ctgtcagcgg cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg
    43261 gtcagtgccg gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag
    43321 atcggcgcat ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc
    43381 atgtgctgga tctacccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg
    43441 ttggagatac cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga
    43501 gaacaggcca actccgcacc tatcgccttg caaagctgtt ctgccagcgc aatgttctct
    43561 ttgctgccaa tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg
    43621 ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc
    43681 acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg
    43741 ctgatggtca gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg
    43801 taaaccatgt gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta
    43861 gacaccgccg ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg
    43921 tttggcaaca gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg
    43981 gcgtaatctt cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg
    44041 ccgagctgga ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta
    44101 gctaaagcct gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc
    44161 cagacttgag aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt
    44221 cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc
    44281 gctgtttcgg cgcggcaacc tgttgttcag accaggcgtc cacggcgtta aaaccaatat
    44341 ccgccgccga ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag
    44401 gaatttgtgg ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta
    44461 aggtttcggt ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga
    44521 taattttgct gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa
    44581 cctgctgggc ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt
    44641 tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt
    44701 catcaatcac cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg
    44761 cgttggtcag ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt
    44821 gctttagctg gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg
    44881 ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca
    44941 cgcacttata gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt
    45001 tcatatcggt ggtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat
    45061 caccaatatt gaaagtcaga tgctattttt ttacccacaa aagaatataa caaactgatt
    45121 aataaccaaa attaacgaaa aacgccctct gttcactcga aacaaaaatg tgataccaat
    45181 cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt ccacgatgtt
    45241 aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt
    45301 cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga atagaaccga
    45361 aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca gtcagagatc
    45421 tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat gtatggggat
    45481 gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg gtgtttggcc
    45541 cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc agttggatct
    45601 ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg attgagatct
    45661 actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga gcgaaagagt
    45721 tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact tacagcttcc
    45781 tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt cgccccagct
    45841 cgacactggt gccgctggca ggtgaaaaac acgccaaagg gttgttcggt accatcgtcg
    45901 acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc cttgccacgc
    45961 cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg caactggacg
    46021 ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc ggtctgcaaa
    46081 aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg ctgggttggg
    46141 tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg gtggggatgt
    46201 tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa ggcggcttcc
    46261 cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc cagatgagta
    46321 tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga
    46381 tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac actctgctgt
    46441 tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt gtggcgcgcg
    46501 ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg ggcttcttca
    46561 tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt
    46621 ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt atcggttggt
    46681 caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg aaaccgattc
    46741 aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg gtgacgctct
    46801 cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa atatcaagag
    46861 gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg
    46921 aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt gacagcgtct
    46981 acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg ctgatccctg
    47041 aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg atggagctgg
    47101 gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc aataccttcc
    47161 tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgcggc accggtaagc
    47221 agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa
    47281 cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt tttattacca
    47341 gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct ccggacaaac
    47401 ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg accaaacttg
    47461 agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat gtggaactgg
    47521 acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg
    47581 accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc gcctttgaag
    47641 atgctgcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt cgtttccagt
    47701 tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa aacatgctgt
    47761 atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca gcgatgtgca
    47821 aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag gtgctgggcg
    47881 gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg cgtgtagacc
    47941 gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc
    48001 aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc aggcctacgg
    48061 gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat gcgctacata
    48121 aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac cgctggccgg
    48181 attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac aaatgttcgc
    48241 agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca ccattcgcgt
    48301 tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt taaatatttt
    48361 caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata tcttcatcga
    48421 agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac tgtggcagca
    48481 caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca ccgaggagta
    48541 caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc tgattcagaa
    48601 cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact tttctggcct
    48661 gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga
    48721 aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact tcatgatgga
    48781 ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc
    48841 cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg tgggcattac
    48901 ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta cgccggaaat
    48961 cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac tggtagaaga
    49021 aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac gctttgccga
    49081 actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca atccacagta
    49141 tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct gcaaagggcc
    49201 gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa tgccctcaca
    49261 tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg acattcagga
    49321 gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc
    49381 ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca atggatatca
    49441 ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac ggtcataaaa
    49501 cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat cttgagttaa
    49561 atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc aaaggcaaca
    49621 aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc gggctggcaa
    49681 aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa agcgagtgga
    49741 tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat cctatgtatc
    49801 aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca gatgtggcac
    49861 ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa cctgccacct
    49921 tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc acctccggca
    49981 ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc gctggatatt
    50041 actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta atgcctgcgt
    50101 ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg gccacctttg
    50161 tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag taccgcgcca
    50221 ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg ccttcagcga
    50281 acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg gagcaggaaa
    50341 aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg atgacggaaa
    50401 ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg tcgattggtc
    50461 gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg ctcccggcag
    50521 gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc aaagagtatt
    50581 ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta cataccggcg
    50641 ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc tgcaatatga
    50701 tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc gccacccatc
    50761 cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat gaagccatca
    50821 aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt tttcgcttct
    50881 gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga aaagatctgc
    50941 cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa cgtcagggaa
    51001 ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca gggatgttgt
    51061 cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat ctgacccgca
    51121 atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt gatgcaaaaa
    51181 ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg
    51241 ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta aaagcggcag
    51301 cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa
    51361 ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt
    51421 tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc ttcgccctgc
    51481 cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc
    51541 tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc gcagaagagg
    51601 cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg gataacgccc
    51661 gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg ctgaaagaga
    51721 tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt cgcagcggcg
    51781 tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg ccgctggcgt
    51841 ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct attacgcctt
    51901 tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat
    51961 tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac tgcgtggtga
    52021 ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca ttatgcatgg
    52081 ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg gagcgatcct
    52141 gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa ttatggatgg
    52201 cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag caggctttag
    52261 tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca gtgttagtga
    52321 cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg tcgggcgctg
    52381 tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc gcccccgttt
    52441 gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg
    52501 gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac gagataaaat
    52561 taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg
    52621 agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc ccgactgtta
    52681 ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag tcgctgacac
    52741 tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct tatttctgtg
    52801 acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga
    52861 atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac
    52921 aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa
    52981 agtctagcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat tgtggatata
    53041 tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa
    53101 aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct catcagagaa
    53161 ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccacgac acgctattat
    53221 ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc ggcattcagc
    53281 gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat aatgcacttt atattgcagc
    53341 gcactgcgac ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt ggtgttgatg
    53401 atgtaggtat attcgccatt cttgatacgg tcctgaatgt gcggacggcc ttcatgcacc
    53461 ttgtttacca gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgggtc
    53521 gcatccagct cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta
    53581 tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc
    53641 agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta
    53701 gagcgcattt ctggccctaa cagcgggtca acgcctggga atttattgaa cggcagcacc
    53761 acttctttca ccgagtagta cggcggaata acttctttgg ttacgccctg ctcagccagc
    53821 gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag
    53881 acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac ttcgttgttt
    53941 ttcaccgcaa actgcacgtt catcagaccg cgcacctgca attcgaaggc cagtttctgc
    54001 acctgctggc gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg cagcgaacat
    54061 gcggagtcac cggagtgcac acccgcctgc tcgatgtgct ccatgatgcc gccaatcagc
    54121 accatttcgc cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaaatga
    54181 tccagcaaca ctggcgcatc gttagacacg ctaaccgccg tctggaagta gcgacgcagg
    54241 tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc
    54301 accagcgggt agccaatctc ttttgccttc tcaactgcca tttcaatagc agtaacggtg
    54361 gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg ttcacggtct
    54421 tccgcacggt cgatagcatc cgggctggtg ccgataaccg gtacgccagc ggcttccagt
    54481 gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc tttcggcttc
    54541 tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta gaggcgatcg
    54601 gaagtgtcgt agtcggtaga gacggtttcc gggttacagt taaccataat ggtttcgtaa
    54661 ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc gataccctga
    54721 ccgatacggt ttggaccgcc gccaagcacc atgatttttt cacggtcggt agacggattc
    54781 gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc aaactctgcc
    54841 gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat attggtcacg cagcttacgg
    54901 atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt
    54961 ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttccgc cactttctct
    55021 tccagacgca ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg
    55081 aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct
    55141 gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg cttccgggtc atccaggctc
    55201 actttcgggt cgaagccagt tgcgcccact tccaggccgc gcagcgcttt ttgcagggat
    55261 tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga
    55321 cggtcgttag caccggcgaa tttttcgaag ttgaagcgag gaattttagt aaccacatag
    55381 tcgatggacg gctcgaagga ggccggagta cgtccgccag tgatgtcgtt catcagttcg
    55441 tctagggtgt aacccaccgc cagtttcgcc gccactttag caatcgggaa accggtcgct
    55501 ttcgacgcca gcgccgaaga acgggacacg cgtgggttca tttcgataac aatcaggcga
    55561 ccgtttttcg ggttcaccgc aaactggaca ttggaaccac cggtttcaac gccgatttca
    55621 cgcagcaccg ccatcgaggc gttacgcatg atttgatatt ctttgtcggt cagcgtttgg
    55681 gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata
    55741 gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc
    55801 cagccgatca gcgactcatc aatcagcaac tctttggtcg gagagagatc cagaccgcgg
    55861 gcgcaaattt cttcaaactc ttcacggtta taagcgatac cgccgccgct accgcccatg
    55921 gtaaaggatg ggcgaataat gcacgggaag cccacgtcag cggcaacagc cagcgcttct
    55981 tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac caattttctt catcgctacg
    56041 tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg cagtggcacc aatcatggtg
    56101 acaccgaact cttccaacac gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc
    56161 tgaccgccca tggttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc
    56221 acttcccagt gaatcggctc gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg
    56281 gtcgccgggt tggagttcac cagaatgacg cggtaaccct cttcgcgcag ggctttacat
    56341 gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc
    56401 agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact
    56461 tagcggtttt acggtactgc tcaattaact tgataaagtg gtcgaacaac ggcgcggcgt
    56521 cgtgtggacc agggctggct tcagggtgac cctggaagct gaacgccggc ttatcgatgc
    56581 gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag
    56641 gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt
    56701 tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct
    56761 tcgcaccgct cgccagcgcc agcagctgat gaccgagaca gatgccgaat accggaatat
    56821 cggtttcgag gaacttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag
    56881 gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt
    56941 gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg atgttgcgct
    57001 tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt ttcgcttctg
    57061 gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct gcggtggtca
    57121 cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaacgcca
    57181 gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca
    57241 gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgcttca
    57301 ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc aggtcgcgaa
    57361 tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggcgtcatta gtgccgacat
    57421 tgccaatatg gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga
    57481 tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg
    57541 cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa
    57601 tcaaaacacc ctccagagaa tactcactca ctttatttgc atattaattc acagtgatga
    57661 cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga
    57721 tatgggcgct aaaagtcaac ttaatggcag gattttttat attattttgt ggctcatgca
    57781 tgttttacag gcattaagcg acaaaaacat catctaacct gttctggaaa acgcttgcgc
    57841 agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat ggtcaaaatt
    57901 tacattaaaa caacaaaaat agaagacgaa acatgtaatt tttggcagtt atagaaaatt
    57961 taaaagcata acaccaaaaa taaaagggct aaaaattaaa gccctttata attaataaat
    58021 taaatgtgtt acttttgcat cataataaac attttgtggt tacaaattat tgagatcaag
    58081 cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag ccgatcttac
    58141 cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca gacgctcgcc
    58201 aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca cggtagcaaa
    58261 accaatggta cctggcacac gttcaccggt atggccttca cgactgtaga ccgcgcaatc
    58321 tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg cggtgcctga
    58381 cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg tgtagtcacc
    58441 catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa cgctaaaatt
    58501 ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt taccggcttc
    58561 gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tgccgacaaa aagcgagatg
    58621 gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat cttttatcgc
    58681 atcaagactg ctttgcacgg taacgcctgt tttccctgca ccagccagct caccggcgtc
    58741 gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg cccagctgca cgccttctaa
    58801 tgccagtgcc gcctgaatca actggcggcc catacgcccc ccggctcccg cgatggcaac
    58861 gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga ccgttttcag
    58921 agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa attaatgata
    58981 aacagggagg gatatcagtg aaaggcatga gtcgatgaat gactgcatgc cgataacatg
    59041 tgacaggtta cggcaccaga gccagcacct cagccaccca ctgttggaag ccttttacat
    59101 tcagatccaa tgccacctgt acattggctg gtttgcccag gcaaccgtcg atatcaacca
    59161 ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc gacaaaacac ggcttgaggg
    59221 tgaataagtc cgggcgcacc agccaggcga tggcgcagag atcgtgcatt cgcagaccgc
    59281 tttgcatact gccgctacgg tagtggctaa acagggcgtg gagcattttt ccggtgcgat
    59341 tcaactccgg taaagtagcg agatagtcag gggttaatat tgcctgattg gtgacatcca
    59401 gaccgcacat gacaatttcg atgccgctac ggaagacaca ggcagccgct tctggatcgg
    59461 cagcgatatt aaactcggca tttggtgtac agttgccgcg accagcagaa ccgcccatga
    59521 tcaccagacg acgaatatac ggtttacatt ccgggcattg taaaagtaac agcgcaatat
    59581 tggttaacgg gccgatggcc accagggtaa cgggctctgg tgcacgcatc agggcatccc
    59641 gaatcgccag aaacgccggt atcccgagtg gctttcggtt gtgctcaaca aagtcgtagc
    59701 cagccattcc cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt accagcggca
    59761 cagcggctcc ctgggcgagc ggaatctccg cattccagaa atgcagcagt tgcagggcat
    59821 tgcgggtagt tttctcaacc gagacattac ccgcgacggt ggtcatcagt tgcaggtcga
    59881 gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg gggtcagtat
    59941 cgaggaagat aggtaaacgc atgttttctt cataaaaaat gccggtaaca agaccggcat
    60001 tttcgcataa cttaggctgc taatgactta atcgacttca cgaatatcga cacgcagctc
    60061 tttcggcact tcgaaaacaa tattttcttc acggccttcc agcggaatgg cttcaccacc
    60121 acccaactgc tgcaaacgtg ccaccacatt ctgcaccaga atatccggag ccgatgcgcc
    60181 cgcagtcacg ccgacgcatt taacctcttt cacccactct tcctggatat ctttcgcatc
    60241 gtcaatcaaa aacgcgtgtt tgcccatacg ctgggccagc tccgccagac ggttggagtt
    60301 ggaggagttt ttcgaaccga ccaccaacac aacttccgcc tgttctgcca gggcgcgtac
    60361 cgcttcctga cggttagtcg tggcgtagca gatgtcatct ttgcgcggac cgacaatttt
    60421 cgggaagcgt ttacgcagcg cgtcgatcac atcagacgtg tcatccaccg acagcgtggt
    60481 ttgggtcata aaggagagct tctcttcgtt tttgaccgtc agtttccaca catcgtctgg
    60541 cgattcgact agatacattc ccccttcggg gttgctgtac tgccccatcg tcccttccac
    60601 ttccgggtgc ccggcgtggc cgatgagaat agattcttcg ccacggcgac tggcgcgggc
    60661 aacttccata tgcactttgg tcaccagcgg acaggtggca tcaaacaccg tcaaatcgcg
    60721 gcttttcgct tcgttacgaa ccgcctgaga aacaccgtgt gcggagaaaa tcaggatcgc
    60781 gccgtccggc acttcgctaa tctgctcaat aaagatagcc ccacgctcgc gcaagctatc
    60841 gaccacgtag cggttatgca ccacttcgtg acggacatat atcggtgcgc cgtaaatggc
    60901 cagcgcgttt tcaacaatgc tgatagcgcg gtctaccccg gcacaaaaac cacgcgggtt
    60961 ggccaacagg atctgcatgt tacgcctcca gtgccggatc gatttccagc acttcaatat
    61021 caaaatgaac ggtctgcccg gccagcggat ggttgaaatc aacggtaatg gagtcgccgt
    61081 taatttcgcg gatcacacca ggcatctcac tgccatccat tgcggtaaaa agcatgattg
    61141 cgccaatttc tggctcgcct gcatccataa attcacggcg ggagaagtac tgaatcaggt
    61201 ccggtgacgg cacgccaaac gccgcatctg gctccagcga gaaggtggtt ttgtcgccca
    61261 ctttcagccc cagcaggtgt tgctccagcc cttcagaaag agaagcatcg cccaggcgga
    61321 acagcgccgg tttaccgttg ttgcgagtag actcggcggt ggtgccatcg tcgagtttta
    61381 gcgtgaagtg caccaggacg gcgctattgc tctgtacaga ttcagacatg caggtttgct
    61441 cttatttttg aggatttttg ccggatgcgg cctacgattt cgtagcaatc tgtaggccgc
    61501 atccggcagg atttattatt gttttttcgc tttggaaggt aaaaaccctt ccagcacgat
    61561 cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga agtgccagtc
    61621 gccgacgtag aagtcgatca tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt
    61681 gcccagcgcg ccgccaataa tcagcgcgta agcgatattg tttagcttct gcgtggcctt
    61741 cgagcgatac atcatcactg ccaggagcac gctaatacca atcgcaatac cggcaaagaa
    61801 ccaacgctgc cagccgccgc tatcggcaag gaaactaaac gccgcgccat agttacgcgc
    61861 ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa agttctggag
    61921 gatcaggtat ttgctgccca gatcgataat cagcacgact accaccagcc acagccagcg
    61981 tagccctgtt gaacagatcg attgactcat caggcaaact tacgtttttc accgtcaccg
    62041 gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc
    62101 tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg ctttactcaa cgcgactttc
    62161 agccctttga gtacttcgct ctgctgagca tcagcaggtg cgtcgttata gtctgcaacg
    62221 gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc ccagcgcggt cagtttcgcc
    62281 gccagttccg gttctgcata caaggttact gccgcttcca gcgagccacc cactttcttg
    62341 tcggcacgcg cttgctcaat gactttgttc acttcgccac gcactttcaa cagctcgtcc
    62401 cagaacgcat cgttcattgc ttcactgtct gccagaccaa acaggccttc gtaccactca
    62461 ccggtgaaga cgtatttttc acgttcgccc ggcagatagc cccacacttc atcagcggtg
    62521 aaggagagga ttggtgccat ccagcgcacc agcgcttcag cgatgtgata cagcgcagtc
    62581 tggcagctac gacgcgccac gctatccgct ttggcggtgt actgacggtc tttgatgatg
    62641 tcgaggtaga aggaacccat ctcaacggag cagaagcgca tcagacgctg taccacttcg
    62701 tggaaatcgt atgcttcgta cgccttgagg atgtcttcct gtgccgcttt cgcacaacct
    62761 acggcccagc gatccagtac caccatctct tccggtttca ccatatcttt tgctggatca
    62821 aaaccattca ggtttgccag caggaagcgc gcggtgttac ggatacgacg atagctatcg
    62881 gcagcacgtt tcaggatctc gtcagaaacg gccatttcac cggtgtagtc ggttgatgcc
    62941 acccacagac gcagaatatc cgcgcccagt ttgttcatca catcctgcgg cgaaacggtg
    63001 ttgccgatgg atttagacat cttgcgaccc tgaccatcca cggtaaagcc gtgggtcagt
    63061 acctgacgat acggcgcttt gcctttcatc gcggtggaga tcattaggga agacatgaac
    63121 caaccacggt gctggtcaga accttccaga tacatgtccg ctgcgtgacc ggcaaattcc
    63181 ggacgcacgt caacaacaga agagtgggta gatccggagt caaaccatac gtcgagagta
    63241 tccggcactt tcacatactg gtcagcttcg tcgccgagga tctctttcgc atcgagatcc
    63301 caccacgcct ggatgccgtc aacttcaacg cgtttagcca cttcttccat cagttcgagg
    63361 gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg tacgccccag
    63421 gtgcgctgac gggagataca ccagtcagga cggttagcaa ccatcgactc gatacgcgcc
    63481 tggccccagt ccgggagcca ctgcacgcct ttgatctctt tcagtgactg cgcacgcaga
    63541 cctttctgat ccatgctgac gaaccactgc ggtgtcgcgc
//
LOCUS       DAJTYL010000008        62680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYL010000008 DAJTYL010000000
VERSION     DAJTYL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.008"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..229)
                     /locus_tag="OO572_000502"
     CDS             complement(<1..229)
                     /locus_tag="OO572_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6659621.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFG"
     gene            complement(393..2234)
                     /gene="nuoL"
                     /locus_tag="OO572_000503"
     CDS             complement(393..2234)
                     /gene="nuoL"
                     /locus_tag="OO572_000503"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6659622.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(2231..2533)
                     /gene="nuoK"
                     /locus_tag="OO572_000504"
     CDS             complement(2231..2533)
                     /gene="nuoK"
                     /locus_tag="OO572_000504"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6659623.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(2530..3084)
                     /gene="nuoJ"
                     /locus_tag="OO572_000505"
     CDS             complement(2530..3084)
                     /gene="nuoJ"
                     /locus_tag="OO572_000505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6659624.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(3096..3638)
                     /gene="nuoI"
                     /locus_tag="OO572_000506"
     CDS             complement(3096..3638)
                     /gene="nuoI"
                     /locus_tag="OO572_000506"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6659625.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(3653..4630)
                     /gene="nuoH"
                     /locus_tag="OO572_000507"
     CDS             complement(3653..4630)
                     /gene="nuoH"
                     /locus_tag="OO572_000507"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6659626.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(4627..7353)
                     /gene="nuoG"
                     /locus_tag="OO572_000508"
     CDS             complement(4627..7353)
                     /gene="nuoG"
                     /locus_tag="OO572_000508"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6659627.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(7406..8743)
                     /gene="nuoF"
                     /locus_tag="OO572_000509"
     CDS             complement(7406..8743)
                     /gene="nuoF"
                     /locus_tag="OO572_000509"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6659628.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(8740..9240)
                     /gene="nuoE"
                     /locus_tag="OO572_000510"
     CDS             complement(8740..9240)
                     /gene="nuoE"
                     /locus_tag="OO572_000510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6659629.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(9243..11045)
                     /gene="nuoC"
                     /locus_tag="OO572_000511"
     CDS             complement(9243..11045)
                     /gene="nuoC"
                     /locus_tag="OO572_000511"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6659630.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(11139..11801)
                     /gene="nuoB"
                     /locus_tag="OO572_000512"
     CDS             complement(11139..11801)
                     /gene="nuoB"
                     /locus_tag="OO572_000512"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6659631.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(11817..12260)
                     /gene="nuoA"
                     /locus_tag="OO572_000513"
     CDS             complement(11817..12260)
                     /gene="nuoA"
                     /locus_tag="OO572_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6659632.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(12891..13829)
                     /gene="lrhA"
                     /locus_tag="OO572_000514"
     CDS             complement(12891..13829)
                     /gene="lrhA"
                     /locus_tag="OO572_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6659633.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            14748..15965
                     /gene="alaA"
                     /locus_tag="OO572_000515"
     CDS             14748..15965
                     /gene="alaA"
                     /locus_tag="OO572_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6659634.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            16049..16648
                     /gene="yfbR"
                     /locus_tag="OO572_000516"
     CDS             16049..16648
                     /gene="yfbR"
                     /locus_tag="OO572_000516"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6659635.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(16707..18539)
                     /locus_tag="OO572_000517"
     CDS             complement(16707..18539)
                     /locus_tag="OO572_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6659636.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(18626..19276)
                     /gene="hxpA"
                     /locus_tag="OO572_000518"
     CDS             complement(18626..19276)
                     /gene="hxpA"
                     /locus_tag="OO572_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6659637.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(19287..19781)
                     /locus_tag="OO572_000519"
     CDS             complement(19287..19781)
                     /locus_tag="OO572_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6659638.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(19864..20319)
                     /locus_tag="OO572_000520"
     CDS             complement(19864..20319)
                     /locus_tag="OO572_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6659639.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            20657..21859
                     /gene="ackA"
                     /locus_tag="OO572_000521"
     CDS             20657..21859
                     /gene="ackA"
                     /locus_tag="OO572_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6659640.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            21934..24078
                     /gene="pta"
                     /locus_tag="OO572_000522"
     CDS             21934..24078
                     /gene="pta"
                     /locus_tag="OO572_000522"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6659641.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            24268..25788
                     /gene="yfcC"
                     /locus_tag="OO572_000523"
     CDS             24268..25788
                     /gene="yfcC"
                     /locus_tag="OO572_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6659642.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(25821..26363)
                     /gene="yfcD"
                     /locus_tag="OO572_000524"
     CDS             complement(25821..26363)
                     /gene="yfcD"
                     /locus_tag="OO572_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6659643.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(26421..26972)
                     /gene="yfcE"
                     /locus_tag="OO572_000525"
     CDS             complement(26421..26972)
                     /gene="yfcE"
                     /locus_tag="OO572_000525"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6659644.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(27028..27672)
                     /gene="yfcF"
                     /locus_tag="OO572_000526"
     CDS             complement(27028..27672)
                     /gene="yfcF"
                     /locus_tag="OO572_000526"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6659645.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            27808..28455
                     /gene="yfcG"
                     /locus_tag="OO572_000527"
     CDS             27808..28455
                     /gene="yfcG"
                     /locus_tag="OO572_000527"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6659646.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            28512..28874
                     /gene="folX"
                     /locus_tag="OO572_000528"
     CDS             28512..28874
                     /gene="folX"
                     /locus_tag="OO572_000528"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6659647.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            28895..29788
                     /locus_tag="OO572_000529"
     CDS             28895..29788
                     /locus_tag="OO572_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6659648.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(29836..30726)
                     /gene="rpnB"
                     /locus_tag="OO572_000530"
     CDS             complement(29836..30726)
                     /gene="rpnB"
                     /locus_tag="OO572_000530"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6659649.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(30923..31696)
                     /gene="hisP"
                     /locus_tag="OO572_000531"
     CDS             complement(30923..31696)
                     /gene="hisP"
                     /locus_tag="OO572_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6659650.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(31704..32420)
                     /locus_tag="OO572_000532"
     CDS             complement(31704..32420)
                     /locus_tag="OO572_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6659651.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(32417..33103)
                     /gene="hisQ"
                     /locus_tag="OO572_000533"
     CDS             complement(32417..33103)
                     /gene="hisQ"
                     /locus_tag="OO572_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6659652.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(33193..33975)
                     /gene="hisJ"
                     /locus_tag="OO572_000534"
     CDS             complement(33193..33975)
                     /gene="hisJ"
                     /locus_tag="OO572_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6659653.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(34196..34978)
                     /gene="argT"
                     /locus_tag="OO572_000535"
     CDS             complement(34196..34978)
                     /gene="argT"
                     /locus_tag="OO572_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6659654.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(35244..35813)
                     /gene="ubiX"
                     /locus_tag="OO572_000536"
     CDS             complement(35244..35813)
                     /gene="ubiX"
                     /locus_tag="OO572_000536"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6659655.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(35908..37425)
                     /gene="purF"
                     /locus_tag="OO572_000537"
     CDS             complement(35908..37425)
                     /gene="purF"
                     /locus_tag="OO572_000537"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6659656.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(37462..37950)
                     /gene="cvpA"
                     /locus_tag="OO572_000538"
     CDS             complement(37462..37950)
                     /gene="cvpA"
                     /locus_tag="OO572_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6659657.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(38209..38871)
                     /gene="dedD"
                     /locus_tag="OO572_000539"
     CDS             complement(38209..38871)
                     /gene="dedD"
                     /locus_tag="OO572_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6659658.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(38861..40129)
                     /gene="folC"
                     /locus_tag="OO572_000540"
     CDS             complement(38861..40129)
                     /gene="folC"
                     /locus_tag="OO572_000540"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6659659.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(40199..41113)
                     /gene="accD"
                     /locus_tag="OO572_000541"
     CDS             complement(40199..41113)
                     /gene="accD"
                     /locus_tag="OO572_000541"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6659660.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(41269..41928)
                     /locus_tag="OO572_000542"
     CDS             complement(41269..41928)
                     /locus_tag="OO572_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6659661.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(42011..42823)
                     /gene="truA"
                     /locus_tag="OO572_000543"
     CDS             complement(42011..42823)
                     /gene="truA"
                     /locus_tag="OO572_000543"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6659662.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(42823..43836)
                     /locus_tag="OO572_000544"
     CDS             complement(42823..43836)
                     /locus_tag="OO572_000544"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6659663.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(43902..45038)
                     /gene="pdxB"
                     /locus_tag="OO572_000545"
     CDS             complement(43902..45038)
                     /gene="pdxB"
                     /locus_tag="OO572_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6659664.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            45137..46132
                     /gene="flk"
                     /locus_tag="OO572_000546"
     CDS             45137..46132
                     /gene="flk"
                     /locus_tag="OO572_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6659665.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(46129..47307)
                     /locus_tag="OO572_000547"
     CDS             complement(46129..47307)
                     /locus_tag="OO572_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6659666.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(47562..48803)
                     /gene="fabB"
                     /locus_tag="OO572_000548"
     CDS             complement(47562..48803)
                     /gene="fabB"
                     /locus_tag="OO572_000548"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6659667.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            48962..50968
                     /gene="mnmC"
                     /locus_tag="OO572_000549"
     CDS             48962..50968
                     /gene="mnmC"
                     /locus_tag="OO572_000549"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6659668.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(51089..51367)
                     /locus_tag="OO572_000550"
     CDS             complement(51089..51367)
                     /locus_tag="OO572_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6659669.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(51401..51949)
                     /gene="epmC"
                     /locus_tag="OO572_000551"
     CDS             complement(51401..51949)
                     /gene="epmC"
                     /locus_tag="OO572_000551"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6659670.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(51949..52758)
                     /locus_tag="OO572_000552"
     CDS             complement(51949..52758)
                     /locus_tag="OO572_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6659671.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(52758..53582)
                     /gene="mepA"
                     /locus_tag="OO572_000553"
     CDS             complement(52758..53582)
                     /gene="mepA"
                     /locus_tag="OO572_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6659672.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(53586..54671)
                     /gene="aroC"
                     /locus_tag="OO572_000554"
     CDS             complement(53586..54671)
                     /gene="aroC"
                     /locus_tag="OO572_000554"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6659673.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(54706..55638)
                     /gene="prmB"
                     /locus_tag="OO572_000555"
     CDS             complement(54706..55638)
                     /gene="prmB"
                     /locus_tag="OO572_000555"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6659674.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            55804..56355
                     /gene="smrB"
                     /locus_tag="OO572_000556"
     CDS             55804..56355
                     /gene="smrB"
                     /locus_tag="OO572_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6659675.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(56459..57349)
                     /locus_tag="OO572_000557"
     CDS             complement(56459..57349)
                     /locus_tag="OO572_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6659676.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(57336..57860)
                     /locus_tag="OO572_000558"
     CDS             complement(57336..57860)
                     /locus_tag="OO572_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6659677.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(57857..58327)
                     /locus_tag="OO572_000559"
     CDS             complement(57857..58327)
                     /locus_tag="OO572_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6659678.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(58324..58872)
                     /locus_tag="OO572_000560"
     CDS             complement(58324..58872)
                     /locus_tag="OO572_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6659679.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(58877..59599)
                     /locus_tag="OO572_000561"
                     /pseudo
     CDS             complement(58877..59599)
                     /locus_tag="OO572_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(59619..60341)
                     /locus_tag="OO572_000562"
     CDS             complement(59619..60341)
                     /locus_tag="OO572_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6659680.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(60401..61555)
                     /gene="yfcU"
                     /locus_tag="OO572_000563"
                     /pseudo
     CDS             complement(60401..61555)
                     /gene="yfcU"
                     /locus_tag="OO572_000563"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(61504..62259)
                     /locus_tag="OO572_000564"
     CDS             complement(61504..62259)
                     /locus_tag="OO572_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6659681.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(62341..62637)
                     /locus_tag="OO572_000565"
     CDS             complement(62341..62637)
                     /locus_tag="OO572_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6659682.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        15474 a        17018 c        15605 g        14583 t
ORIGIN      
        1 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
       61 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
      121 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
      181 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
      241 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
      301 cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac ctacgttctt
      361 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
      421 accacgaccg caccgatgct catggatgcc acataccagc gcagataacc gttctcgctt
      481 aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat cattgagttc
      541 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtcg
      601 tacagccagt caaagcccca agcgttgtac caccaggtac tcagcaggcg gcccggcgca
      661 ctgttggcga tggaggtcac cagagaacgt ttacccagcc acagccaggc tgccagcaga
      721 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
      781 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
      841 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
      901 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
      961 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     1021 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag tgccgacagt
     1081 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     1141 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     1201 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     1261 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     1321 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     1381 atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc
     1441 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
     1501 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     1561 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     1621 ggtgccagtt ccaccatatc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     1681 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgctgct
     1741 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     1801 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     1861 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     1921 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     1981 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     2041 cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     2101 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     2161 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     2221 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     2281 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     2341 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     2401 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     2461 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     2521 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     2581 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     2641 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     2701 tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga ggatggcgta
     2761 aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca cctgcggttt
     2821 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     2881 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     2941 agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     3001 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     3061 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     3121 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     3181 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttc
     3241 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     3301 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttaatg
     3361 cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     3421 ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc gccgtccggg
     3481 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     3541 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     3601 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     3661 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     3721 ttttccagcc gaaggacatt acccggtcat aacgcggacg cggtaacgac gcacgaatca
     3781 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     3841 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     3901 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     3961 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cacaccgcca
     4021 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc cacacatgcg
     4081 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     4141 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     4201 gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     4261 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     4321 tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     4381 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     4441 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca taacggttct
     4501 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag gtgacaacca
     4561 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     4621 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     4681 aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt cggcgatttc
     4741 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac ctgcgttcac
     4801 acccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     4861 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt acggcgcgat
     4921 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     4981 gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     5041 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     5101 cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     5161 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     5221 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     5281 cggcgcagca tctttgatac ccgccagttc tgggattttc gccacaacag cgtcaattac
     5341 atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca gcgagtgcag
     5401 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat cgtaaacctg
     5461 gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt cagcaaagct
     5521 ggcagaggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat gatcaaccac
     5581 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag cgtaacgatg
     5641 cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt cggttaacgc
     5701 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
     5761 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
     5821 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
     5881 tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga taccgtcaac
     5941 cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
     6001 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat caacgttggt
     6061 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
     6121 agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct gacgcactgc
     6181 cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
     6241 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
     6301 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
     6361 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
     6421 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
     6481 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
     6541 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
     6601 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
     6661 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
     6721 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
     6781 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg cgccataaac
     6841 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
     6901 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
     6961 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
     7021 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
     7081 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
     7141 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
     7201 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
     7261 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
     7321 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcggtcag
     7381 ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
     7441 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
     7501 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
     7561 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctggcctt
     7621 cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
     7681 tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca
     7741 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg cccgctttac
     7801 cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag tcagtccccg
     7861 cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg gcgtaatctt
     7921 cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga tttttcaccc
     7981 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
     8041 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
     8101 aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag
     8161 cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
     8221 ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca
     8281 agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt
     8341 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
     8401 cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag gtgcccggct
     8461 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
     8521 ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
     8581 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
     8641 aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
     8701 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
     8761 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatgtttgg
     8821 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt
     8881 cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac cctgataccc
     8941 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggctggcg
     9001 gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac
     9061 atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt
     9121 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
     9181 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
     9241 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
     9301 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
     9361 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
     9421 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
     9481 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
     9541 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
     9601 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
     9661 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
     9721 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
     9781 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
     9841 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
     9901 tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
     9961 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    10021 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    10081 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    10141 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    10201 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    10261 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    10321 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    10381 ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc cgcgtttcat cccccactct
    10441 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    10501 ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    10561 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    10621 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    10681 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    10741 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    10801 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    10861 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    10921 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat taccggatca
    10981 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    11041 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    11101 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    11161 aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    11221 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    11281 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    11341 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    11401 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    11461 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    11521 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    11581 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    11641 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    11701 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    11761 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    11821 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    11881 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    11941 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    12001 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    12061 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    12121 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    12181 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    12241 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    12301 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaataactg
    12361 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    12421 caactttaac aaaagcttct tcacattagt ttacataata tcaacaccat tagcatttaa
    12481 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    12541 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    12601 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca taacaaaaac
    12661 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    12721 tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt ttgtacgttc
    12781 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    12841 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    12901 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    12961 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    13021 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    13081 agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct
    13141 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    13201 tctactttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    13261 acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    13321 ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    13381 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    13441 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    13501 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    13561 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    13621 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    13681 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    13741 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    13801 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    13861 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    13921 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    13981 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    14041 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    14101 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    14161 aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    14221 accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca
    14281 aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa ataataatta
    14341 attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct tacccccatt
    14401 gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat atcatcatcg
    14461 cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac agataaaaaa
    14521 accagacttg caattaagaa ttagaacaat tcactatata acattgcatg taaagcatat
    14581 aagcctaatt atttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
    14641 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
    14701 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
    14761 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
    14821 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
    14881 cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
    14941 ctcaagggta ttgcgattcc aaagggcttt actccgcgcg taaagccatc atgcagcact
    15001 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
    15061 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
    15121 ctgcaccaga ttacccactc tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
    15181 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
    15241 ttacgcctcg tacgcgtggg attgttatta tcaacccaaa taacccaacc ggcgcggtat
    15301 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
    15361 tcgccgatga aatttatgac aaaattctct acgacgatgc tgagcatcac tcaattgcgc
    15421 cgctggcacc tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg
    15481 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
    15541 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
    15601 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
    15661 gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct
    15721 gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca
    15781 ttcatgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
    15841 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
    15901 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
    15961 tgtaatctta atttcactgc cggagattgc atccggcagt gttatcccgc cacaatgacc
    16021 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
    16081 ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc
    16141 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa
    16201 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
    16261 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaatgcgc aaatcgctca
    16321 ggagtacaag gctattgaaa aaatcgctca gcagaaactg gtcgatatgg ttccggaaga
    16381 actgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
    16441 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
    16501 cggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg
    16561 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
    16621 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat cagtcaataa
    16681 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
    16741 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
    16801 taatttcccg gacctaaaac cagggtgtta acaggtgaag acaccggtgt cataaaggcg
    16861 gcggatgctg ccatcgccac gaccatcgca aatggataag gcgacactcc catcgttttg
    16921 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaacagc
    16981 ccaataaccg ccgacaagac aaacaaacac cccagcatca tatgtggccc ataaccgccg
    17041 ccaatatcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
    17101 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
    17161 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
    17221 ggattaggaa tttcatttgt cagcattaac gccaccatta gcaccagaca gaaaatggca
    17281 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
    17341 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
    17401 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
    17461 cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
    17521 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
    17581 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
    17641 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
    17701 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
    17761 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt
    17821 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
    17881 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
    17941 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
    18001 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
    18061 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt
    18121 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
    18181 ggaataaaga tagcgacaac gctggttgaa ctcataaacg cgccaagccc cgcgacggtc
    18241 agcatcaaca aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt
    18301 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
    18361 atcaggacaa cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg
    18421 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
    18481 gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
    18541 acttatcctt gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga
    18601 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
    18661 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
    18721 ctgccggagc gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag
    18781 cgggagcatc ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta
    18841 acagatacgc atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct
    18901 ctggtgcggg aagccccgct attttatggc gcgctcgcgc taccggcatg gagccagaag
    18961 tcacaatggc ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgccccag
    19021 gaagcgcggt aataccttcg gtttccgtgg cctcgatttg ctccagacgc gtaaactcgg
    19081 cggcaatatc agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt
    19141 taccgtgaat gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc
    19201 tccacgcccg ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa
    19261 aacctttgca ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg
    19321 ctcaaatggt actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc
    19381 cacattggcg tctgggcgtt aaaaccatga gtcccagcgt caaaatgggt atagcgccct
    19441 tccacattaa ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag
    19501 ccgaggaagg tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaagaa
    19561 acatacaacg catgatacat atccataatg tcgatgatag tgcggcaggt ttcttctttc
    19621 agctcgccaa actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt
    19681 gtttgcaggc gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac
    19741 tgattagaca aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact
    19801 ctactacggt gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt
    19861 caattacaaa tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc
    19921 agctaatgcc tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg
    19981 caatttaccg cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga
    20041 acgcttgccc agccaccaca atccctgcat gggtaaactt aaggcgaaca gtgcagtggc
    20101 aactgccggc ccaagcagac cgcccagggc aatctgccag cagagagtaa atacggcgat
    20161 cggcggcata aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac
    20221 cggagcaagg cgtttttcca gtggccacgt ctttgagtaa tgctgtccgc ggcgaaacaa
    20281 gctaaaaaaa ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata
    20341 taaagattca aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg
    20401 tcttccatac ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc
    20461 attgagtcgt caaattcata tacattatgc cattggctga aaattacgca aaatggcata
    20521 gactcaagat atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa
    20581 atgtcggtgt catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc
    20641 aattataggt acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
    20701 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
    20761 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
    20821 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
    20881 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
    20941 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
    21001 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
    21061 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
    21121 gactatgccg gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg
    21181 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
    21241 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
    21301 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggtc tgaccccgct
    21361 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
    21421 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
    21481 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
    21541 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
    21601 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gcattcactg gtggtatcgg
    21661 tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
    21721 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
    21781 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
    21841 gagccgcctg actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc
    21901 cgccaaatcg gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct
    21961 accggaacca gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc
    22021 aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg
    22081 cccgatcaga ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg
    22141 ctgaaaatga gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa
    22201 gagatcgtcg caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt
    22261 ctggtcccga cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg
    22321 ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa
    22381 gagcgtatcg aactgacccg caacagcttc ggtggtgcca aaaacaccaa catcaccggc
    22441 gttatcgtta acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccggatctg
    22501 tccgagattt tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg
    22561 caagaatcta gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg
    22621 actcgtgcga tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc
    22681 aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag
    22741 cacttccgtg ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc
    22801 gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac
    22861 gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta
    22921 tttatggtga acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa
    22981 gttccggttg acgatcacga acgtatcgag aaagttcagg aatacgttgc taactacatc
    23041 aacgctgact ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg
    23101 ccagcgttcc gttatcagct gactgaactg gcgcgcaaag cgggcaaacg tatcgttctg
    23161 ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc
    23221 gcaacttgcg tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt
    23281 gtagaactag gtgcaggcat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt
    23341 ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag
    23401 ctggaagaca acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg
    23461 gtttccggtg ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa
    23521 actgcaccgg gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt
    23581 tacgtttacg gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc
    23641 gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc
    23701 tcctactcca ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact
    23761 cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct
    23821 gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct
    23881 accgtgttca tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt
    23941 tctgccgacc tgatctccat cgggccgatg ctgcagggta tgcgcaaacc ggttaacgac
    24001 ctgtcccgtg gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag
    24061 tctgcacagc agcagtaatc tcgtcatcat ccacagcttt gcgctgcgga tatctgaacc
    24121 ggaaataatc actatttccg gttttttatt ctcttaattt gcattaatcc tttctgatta
    24181 tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc actttgcata cttaccactt
    24241 tgttttgtgc aagggaatat ttgcgctatg tccgcaatca ctgaatccaa accaacaaga
    24301 agatgggcaa tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagtctt
    24361 gccacctggg tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat
    24421 ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa
    24481 gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc
    24541 ctgatgaact tcccgtttga agggttaacc tcaggatcga aatacgggac agccgttggc
    24601 atcatcatgt ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc
    24661 attgataacg gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt
    24721 cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc
    24781 gtcgcctttg ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc
    24841 accgtcctgg tgacttatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg
    24901 ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg
    24961 cgcatcgtgg tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc
    25021 tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt
    25081 gaaaagcagg cggatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt
    25141 gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt
    25201 ccagaaattg ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt
    25261 ttccgcctta acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga
    25321 atgatgatcg cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggttggt
    25381 aatggtgaag caggtaatgc cagcgtgtta aatactatcc tcaacagcat tgccaatgcc
    25441 attagcggtc tggacaacgc ggtcgcggcc tggtttatgt tgctcttcca ggctgtattt
    25501 aatttcttcg tgacgtccgg ttctggtcag gcggcattaa ctatgccgtt actggcaccg
    25561 cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc tggcgttcca gtttggtgat
    25621 ggcttcagcc acatcattta cccaacctca gcttcgttaa tggcaacgct cggtgtgtgc
    25681 cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc tgcttggact gctgtttatt
    25741 atgtccagcg tcgtagtgat cggcgctcag ttgatgggct accactaaaa atgttaagag
    25801 ccgcaattgc ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca
    25861 ttgcgcttca tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt
    25921 gcggtgattt cttccggcgt cagccagcaa actccactga cttcatcttc ctgtagggcg
    25981 aagggaccgt gagagacgca actgaacaac gcgccccaga cacggcaatt tttatcttcg
    26041 aaatagaact gcccgtgctc ggcaaagggg acaccggcaa tgcccaactc ttcttccgct
    26101 tcgcgacgcg cggattccag cagttgctca tcggcctgaa ctacaccgcc tgcggtcgca
    26161 tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc
    26221 atgccatcat gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt
    26281 tcccggctgg cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta
    26341 cttgccaaac gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg
    26401 cattttgagt tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca
    26461 ttgagtgcga ttacactaag tacgtcatta tccagcatgc cataacccgc cggattaccg
    26521 cctttcggaa tactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc
    26581 actggcagat gggtatgacc gtacaccagc acatcgttct ggtttaaagc aggtagattt
    26641 tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt
    26701 tgccacggcg cggttatcgg gaaatgcagc atcatttgat ccacttcgct gtcgcagttg
    26761 ccacgcacag cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc
    26821 gcgtaaccct ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc
    26881 agccactggg caccgctttg gacaaacaac tccacaacac gttccgtcgc cggtaacgac
    26941 ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag
    27001 ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa
    27061 acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac
    27121 ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca
    27181 ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca tcgcgaacag
    27241 tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac
    27301 aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat
    27361 ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg
    27421 cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc
    27481 gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg
    27541 ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc
    27601 cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc
    27661 gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt
    27721 ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt
    27781 tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat
    27841 ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta
    27901 gacttgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa
    27961 attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag
    28021 gctgctgcca ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg
    28081 cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg
    28141 cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa
    28201 cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagcggct ggaaaactcg
    28261 ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat
    28321 gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag
    28381 cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag
    28441 cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg
    28501 gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta
    28561 cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga
    28621 cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt
    28681 atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg
    28741 aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg
    28801 aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa
    28861 gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac
    28921 gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaattacggt
    28981 agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga cactatggca
    29041 agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga
    29101 accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc aaagccgctg
    29161 gaatatcacg caaaaactgg tcgatttgat taatgctagc gacacgccgc cgtcggtact
    29221 catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga
    29281 ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg
    29341 ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc
    29401 ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat
    29461 tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct
    29521 ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct acccggtacg
    29581 caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta ttttgcgcgt
    29641 ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat taggtggaca
    29701 acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga
    29761 agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact
    29821 ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac
    29881 catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc
    29941 ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag
    30001 tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg
    30061 ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    30121 ccctgtaact aacagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    30181 atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg
    30241 caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg
    30301 ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata
    30361 aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat
    30421 tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    30481 ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc
    30541 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt taagcgtcgt
    30601 taaatcacac agtttgcgca gcggcgcggg aagatgaata ttaataaaat tccgcgcggt
    30661 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    30721 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca
    30781 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac
    30841 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    30901 gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt tgcagacgag
    30961 ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt ttcccctgat
    31021 ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca
    31081 ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg cctaccagtt
    31141 caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttctggttcc atcgccagcg
    31201 cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga tatttcccct
    31261 gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg cgcgcttcct
    31321 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac accgtcatat
    31381 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt
    31441 gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg atcgtctggc
    31501 cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg
    31561 tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca
    31621 gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg ataacgttta
    31681 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    31741 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    31801 gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt
    31861 ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag catcaggatc
    31921 acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    31981 tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc atgcggaacc
    32041 gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    32101 agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt tcccttaaca
    32161 atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    32221 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact ggagacacga
    32281 ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac
    32341 agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat
    32401 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa
    32461 ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg ccacagacga
    32521 tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct ttgaccacat
    32581 cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc
    32641 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc
    32701 cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact gccataaacg
    32761 cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataataccag
    32821 cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc atgtttagcg
    32881 caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca
    32941 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    33001 cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc tccagcgtga
    33061 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    33121 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt
    33181 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    33241 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    33301 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    33361 cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt cgctggcagc
    33421 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    33481 ctgcccctga tacgagacga tttcaatgcc ttttggtgcc cagtgttcat taccgaacgt
    33541 ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    33601 ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    33661 gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    33721 cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa acgtacattg
    33781 cgtattgatg cgcttgcaca actccttcgc cagctcgata tcgaaaccaa ccagttcgcc
    33841 ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg
    33901 cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    33961 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    34021 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    34081 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    34141 aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg acgtatcagt
    34201 caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac
    34261 gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcataatct ttacgtagcc
    34321 ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaagcga
    34381 aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcttgtaacg
    34441 cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca
    34501 ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg gttgatccct
    34561 gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt
    34621 tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct
    34681 gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc gctttcagtg
    34741 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    34801 acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca tctttcgacg
    34861 agaacagcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc
    34921 tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac ttcttcatac
    34981 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    35041 ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt
    35101 aataagacat tgcattattg tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca
    35161 gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    35221 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    35281 agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    35341 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc
    35401 gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    35461 aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    35521 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccagcgtc
    35581 tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgagcgt gacatcagct
    35641 aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc
    35701 tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc
    35761 acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt
    35821 tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc aagtcagggt
    35881 gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    35941 ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga ggaaatcgag
    36001 gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg
    36061 ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat cctggaaaat
    36121 caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt gagcgatcag
    36181 ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc
    36241 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    36301 agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc ggaactcggc
    36361 gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat
    36421 aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    36481 acgagcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca ccacgtcgat
    36541 atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca tattcacacg
    36601 cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt atacatactc
    36661 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    36721 ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt
    36781 atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg
    36841 ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca taccgtggcc
    36901 gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg cagcaatggc
    36961 agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct cgctggcgaa
    37021 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    37081 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    37141 atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    37201 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    37261 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    37321 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    37381 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    37441 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    37501 atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt tgctccagtc
    37561 ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa
    37621 cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    37681 tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat
    37741 aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    37801 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    37861 cgataacgct tcacgaacaa agccgtggat caggctaacc agagaggaaa aagcaatcac
    37921 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    37981 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    38041 catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg
    38101 catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg gttctaacac
    38161 tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt aattcggcgt
    38221 atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt
    38281 cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg
    38341 cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt
    38401 cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac ccgttggcgc
    38461 tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggta cttcaacctt
    38521 tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag gttccggttc
    38581 aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg cggcatcacc
    38641 tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc
    38701 tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga tagccgcgaa
    38761 ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag
    38821 cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc
    38881 tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac accagcacgg
    38941 tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg
    39001 atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca
    39061 gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat
    39121 ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca
    39181 tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg
    39241 gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa
    39301 tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat
    39361 tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg
    39421 cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac
    39481 ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg
    39541 gtttttcgct gcggaagatg cctgctttct cgagaccaat actttcgcga tctggaccca
    39601 gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca tcgacaatat
    39661 tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct
    39721 gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac
    39781 cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcctggc
    39841 cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag acgcccactt
    39901 tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg
    39961 tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc gccgcgacct
    40021 ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa
    40081 gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg
    40141 attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc
    40201 aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag
    40261 gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt
    40321 ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga
    40381 atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga
    40441 ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac
    40501 cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg
    40561 ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg
    40621 cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc tgctcaacgg
    40681 cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga
    40741 atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt
    40801 tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa
    40861 acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta
    40921 acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga
    40981 cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag
    41041 tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt
    41101 caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg
    41161 gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa
    41221 cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc
    41281 gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa tagaaaccac
    41341 aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg taccgaagaa
    41401 atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt tataagcagc
    41461 gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg ttctgacgat
    41521 cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa actgatgggt
    41581 tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata acttttcacc
    41641 gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga tcagcatcag
    41701 cactaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg ctccggcaac
    41761 aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt cacagaacaa
    41821 aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct ctgccagatg
    41881 cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc
    41941 cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc
    42001 attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc
    42061 ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgcca
    42121 gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac
    42181 cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt
    42241 tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata acgttgcgcc
    42301 acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca
    42361 agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc
    42421 ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag
    42481 ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga
    42541 tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg cgctgcgcgg
    42601 ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc
    42661 agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca
    42721 cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc
    42781 ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca gatactcctg
    42841 caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc
    42901 caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc agccaacaga
    42961 aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct cttcagagag
    43021 cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag
    43081 tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc
    43141 cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc
    43201 ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg ccagttgacg
    43261 accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc
    43321 taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat
    43381 acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag
    43441 actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac
    43501 ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc tgtcgatcac
    43561 caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt tgcctgcgac
    43621 aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca cggtgattgt
    43681 tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc
    43741 cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca tagcgccagt
    43801 tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa
    43861 agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc cggatgatga
    43921 acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca
    43981 tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc
    44041 ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc
    44101 accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg gccaaactca
    44161 ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa cttgctatag
    44221 gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc tgcgatatgc
    44281 ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc gccttcccag
    44341 acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag cgcggtatta
    44401 tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag gctacggatc
    44461 agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag tggcgtatgg
    44521 aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc accctcatcc
    44581 ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc ttccagtcgc
    44641 gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg gtcgtgcagt
    44701 gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc caccaccgca
    44761 atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca
    44821 tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt tcctgccagc
    44881 aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga
    44941 gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct aaataagtcg
    45001 cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac
    45061 gagagttaac cggacaagta tgccataatc tcgcggccag gcatacttgc gaagatttca
    45121 ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc agccaccagg
    45181 tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac
    45241 ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact
    45301 gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca
    45361 cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc
    45421 tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaatc atcgtcaggc
    45481 ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga
    45541 gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc cgcaaccaca
    45601 gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc taaaacagct
    45661 ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc aatcgatgct
    45721 ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt
    45781 gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc tggaatcact
    45841 acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat
    45901 ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat
    45961 tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct
    46021 gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct
    46081 gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt aaccccgacg
    46141 aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg caagaaatac
    46201 cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga cgccgagggc
    46261 gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg
    46321 tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc
    46381 cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca agcccaccgt
    46441 ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc
    46501 aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat
    46561 cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac
    46621 gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc tccacagcga
    46681 tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag
    46741 taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc tatgaatcaa
    46801 caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt tccatgacat
    46861 cactttgcca gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag
    46921 taactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa
    46981 cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc
    47041 taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc gcccggcgta
    47101 accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga ccatggtatt
    47161 gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca accctacggt
    47221 catgtaggtg agaaaaaccg caaaagagat gcggaagagc gaaaaactgg cagaagatcg
    47281 tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag
    47341 tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta
    47401 cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tagaactggt ggcgattgtc
    47461 aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcattg
    47521 gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg cctactgcga
    47581 attaatcttt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa gccgaagctg
    47641 ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt cagacccgca
    47701 gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg ttccagcatc
    47761 agcagagagt agatagcttc ctgcacaccc gcagcgccca gtgagtgacc ggtcatggct
    47821 ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc tgccagctct
    47881 ttcacgtcgc caaccggagt cgaagtaccg tgagagttca ggtaatcgat tggggtatca
    47941 acgccatgca tcgccatctg catacaacgt actgcgcctt cgccagacgg agcaaccatg
    48001 tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgta agcaccacgc
    48061 gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc gataacgaaa
    48121 ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt gtatttagta
    48181 gatagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc ttcgccgccg
    48241 ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt accgatacag
    48301 tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat tttaaacggc
    48361 gtggcgagac aggcagaaac gccggatgcc atcgctttag tgaccacata cgggccaacc
    48421 gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa acgcggggaa
    48481 ccgccgccgg aacctgcaat caggccaacg cgcgggttat tctggtaagc ttccggagag
    48541 aggcccgcat cagcgattgc ctgctccata gaaaggaatg cataaatgga tgcgtcgctc
    48601 ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttat gttgccccag
    48661 acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc tgaacgtcct
    48721 tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac aatgcccagg
    48781 ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga gtaagtttcg
    48841 aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt aatagagata
    48901 tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac gagtaactta
    48961 cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg gtacacctgt
    49021 ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag agacgcgtta
    49081 tgtttttctg ggaggtaacc aattagaggt acgctttcct gagcatccac atcctctgtt
    49141 tgtgatagca gagagcggct tcggcaccgg attaaacttc ctgacgctat ggaaggcatt
    49201 tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt tcattagttt
    49261 tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact ggccggaact
    49321 ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg gttgccatcg
    49381 tttattgctc gatgaaagcc gcgtgacgct ggatttatgg tttggcgata ttaacgaact
    49441 gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc tggacggctt
    49501 tgcgccagct aaaaacccgg atatgtggac gcaaaatctg tttaacgcca tggcaaggtt
    49561 ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc gccgcggttt
    49621 gcaggaagcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac gggaaatgct
    49681 ttgtggggtg atggaacaga cattaccact cccctgttcc acgccgtggt ttaatcgtac
    49741 gggcagcagc aaacgggaag tagcgattat cggcggtggt attgccagcg cgttgttgtc
    49801 gctggcgtta ttacggcgcg gctggcaggt agcgctttat tgcgcggatg aagcccccgc
    49861 gctgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca aacacgatga
    49921 ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt acgaccaatt
    49981 acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg atgagaaaag
    50041 ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg ctgtagccgt
    50101 tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg gcattactta
    50161 tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg aactggcgca
    50221 acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc gtaaggatga
    50281 ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag tggtactggc
    50341 gaacgggcat caaatcagcc gtttcagcca aacgtcgact ctcccggtgt attcggttgc
    50401 cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc aggtgctgtg
    50461 ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta ttggtgccag
    50521 ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc agaatcgcca
    50581 gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg tcagtgataa
    50641 agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg taggcaatgt
    50701 tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaaa aagatgaggc
    50761 ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt ctcgcggttt
    50821 gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg aaccgattcc
    50881 gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc ggaaattgtt
    50941 gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg gatgcggcgt
    51001 gaacgcctta tccaacctac gtgtgacgtg taggcctgat aagacgcgat aagcgtcgca
    51061 tcaggcatgg tgctccagac gccgcaactt acagctgtga cgcctgctga aacaggtttt
    51121 cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca ccaatggctt
    51181 tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca tcaccacttt
    51241 ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca aacaactcat
    51301 catcactggc atggtcaacc atgccgtcga ttaatgccag aatgcgtgat tcaaactccg
    51361 cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct tcagttgagc gcttccggcc
    51421 acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt gctttaatga
    51481 agcgtgccgg gcgttccgga atgccatttg tcagataatc catcacctgc gcatgtaccc
    51541 gccgttgaaa caccacacga tccggttcga aatccccttc cagattgtcg caactgacgt
    51601 taaacggata tccagccgcc acgcagaata accagtcgaa cgcctgcggc ttcacttcaa
    51661 catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc cgggcaatac cagtagccga
    51721 aatcaaccag ctcacggcgc gcttttccgg caatacacca gtgcgaaatc tcatgaattg
    51781 cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc gcatcagcag
    51841 gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca tcggcaaagc
    51901 agctattaaa aatttcaatc aactgctcgt agtggtgtgt actgttcatt agttcatccc
    51961 caaccagtgg aggatctcct gtccgtggct atcgtaaagt aatttggcac tcatcaccgc
    52021 cgagacgata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca ccaaccgtga
    52081 acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg cccaaatcac
    52141 tttgccgccg agaataaaca gcagcagacc gccaatgttt gacgttgcgt tgagtaattt
    52201 ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc ataacgaagg ccagcgcgta
    52261 aaacgatccg gcagccgggc caaagaatcc gtcataaaaa ccgacgcagc caccggcaat
    52321 taacgcgaag ggtaaaccgt acatccggcg ctggcgatct tcttcaccca gctttggcat
    52381 cagcaagaaa tagaggccga tacaaatcac cagaatgggc aaaatctgcc gtaagacatc
    52441 agcctgaacg tactgtacca acagtgcgcc gctcattgag ccgacaaagg tcatggcaat
    52501 attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga tagtggcgga
    52561 aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcgttagcgg gagacattcc
    52621 cgcagccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg agtcgataaa
    52681 tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggagaaa ccataaacag
    52741 gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca ggaaggcggc
    52801 aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg ttcaaaccag
    52861 ctttgcagtt ctgccccgca accatcgcct gatggcggta aaggttgatc ttcacactcc
    52921 agactatcgg cagggcaacg taatcgtaca tgcatatgcg cgcgatgctg gaaccagggt
    52981 cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg ttgtttaatc
    53041 gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg cggcgagttt gatcaagctg
    53101 aaaatttctg gcttccacag cgtggagaca acgtgtttac cgtcgcggga aactaagtcc
    53161 agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag ttgcagaaag
    53221 atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg cccaccagcg
    53281 ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac ctggctactc
    53341 agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg atcggtacgc
    53401 atgacctgat aatgttcgga ctgtatcggc aacgtgtcag cgccgacaat acagccatta
    53461 gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt ttgccacggc
    53521 gtcgctgcca ggctagcact actggcaagc agagccagca gcgcaatcgc ggttttgttc
    53581 attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg ctgccgtaac
    53641 aggtgatcca ttaaaacgat cgccagcatc gcttcagcga tcggcactgc acggatcccg
    53701 acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa gcggttaata
    53761 gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgagc aatgatttgc
    53821 tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt tgctctggaa accgtctttg
    53881 gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc gtcgccaatt
    53941 tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc atccaggcgg
    54001 tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt gactttcgcg
    54061 ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc gtcgattttg
    54121 tcggggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt gatttccagc
    54181 ggaatgtcgc ccatctgggt caggcagccg cggatttcaa taccaaattt ctcggcgaga
    54241 tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc ggaagaacgt
    54301 ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc agcatggcct
    54361 ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac gctggtcggt attttcgatc
    54421 aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag tattttgacc
    54481 tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg acgatcaagg
    54541 tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc gacgatgcag
    54601 ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg tccaattgtg
    54661 tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat ctttataaat
    54721 cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg agcataaaca caccatcgcc
    54781 gccgttatca aactccagcc aggtgaacgg aacatcggga tattgttcca taagatgtac
    54841 catgctgttg cccacttcac aaatcaacac gccatcatca gcaaggtaat ctgccgcgtt
    54901 accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc ccagttccgg
    54961 ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat acggcgggtt
    55021 agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg aacgaatcgg
    55081 aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg ccagcgcgtc
    55141 gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac aggcaatggc
    55201 gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc tgatgagtcc
    55261 ggcaaattta ttgttgatca gttcaccaat cggcgagcgc ggcaccagca cgcgttcatc
    55321 gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca ccggaatgcg
    55381 ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg aggtcagacg
    55441 cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta acaccagctg
    55501 tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg ccgcgctgaa
    55561 gcggcttacc gaccagcgca atatgtcctg aatggtttgc agctcattta ctgcttcatc
    55621 aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc ggcggctagt
    55681 ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa aaatacgttt
    55741 aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa tagagacaga
    55801 taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg ccagttgatg
    55861 gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg taaaaaaatc
    55921 agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca ttatttctcc
    55981 gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg cccggatgtc
    56041 agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt gtttttggat
    56101 ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat tgccgcctgt
    56161 cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca tattttgaag
    56221 caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca ccaggcaccg
    56281 aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga gtggttgccg
    56341 ccggagttgc cctgaatgct ggcagcaaat gccggagcag cgtaaacgcc ttatccggcc
    56401 tacaaatatg tgataattca ataaattgca attcacttcg taggcctgat aagacgcatc
    56461 agagcaatac tacgaacttc agttcattca atacatactg aaaacgacac gtcgaatcaa
    56521 tcggttgccc actactggta ttcacattca ccacaatatc gtgtagtact ttatccgtta
    56581 ccggatgacg agttggcgta accgttaccg ttgatttatc acaaccgtaa gaggtaaagg
    56641 ttaacaattc tgataccgcg cggcagtatg ctcctctgcc atcattatca gaaaaattac
    56701 tgccatcaca gagtctgccg ccttccatca ggtaggtcga aagcgtggtg gtcactgttg
    56761 accccgtgga ctgaattcgt aacgccttat tatggctgac ataattttca ctccaggaga
    56821 tatcgtatga agcgaccggt atcgttgggc atattgtgtt atgcactgta ggggccgtat
    56881 agcttccaaa tccgggtaaa tctgtatagg aataaaagga tgcgatccaa gggcaaccat
    56941 aatcatcaga tgcaacggta attttaaacg tcatatatgc acaacgaaaa attttcgtat
    57001 ctatgccagg gcaatttgag ttattaattg tatcaaagtc agtcagtacc aaagttggag
    57061 ctgaacccga cccagaccag gaaataaccc gcaacgtatc gcctttatac gcaaaagaat
    57121 catatttccc atatgcaacc tcctttggcg ttatcaccgt aacattgaaa acactgctcg
    57181 ctacagcacc ggttaccttg tccatggcat aataaactct cacactgtca aaactcgcac
    57241 tcataagttt atctccagca taactcttag atgcgccgac aacactcaga acgagcagca
    57301 ttaggctcgc gaaaattatg gtccattttt tcatttcaga aatactcaaa ggttgccgtc
    57361 actgtggcac taaagtctcc ggacgttaca tctcgcccgc tctttgcctg taaccaggta
    57421 ttaaaattta ggctgttatt tccctgattc agcgggagcg tgacgcctgt tgtattgttg
    57481 attggcaccg atgtgcctgc cgcagtttca ataccaatcc cagcgccgga tgctgatgac
    57541 gaggagtcaa aggccagaaa gccgggtaga gcactatctt ctgttcctgt caacgttact
    57601 ttgacattaa acaatgtaga actatgacaa tctttcaatt gaaacaccaa tggcactcgc
    57661 tctgactgcc caaggttcat taaatcactg gcggcaatgg caggaaaatg tacttctgcc
    57721 aactggctac cattaatcac tggcgtacac gatttactga ttaaattgcc ggtaaagtgc
    57781 aaattatctc ctgctattgt ggttccactg cccagcaaca tcaggctact aataaaaaac
    57841 aacgtacgct ttttcttcat tggtaatcta cctgtaacgt ggcccaggcc tcaaagtccg
    57901 cctctggcag aatgacacca ctctttttta ctggcacagc cgttaacacc ggtaaatcgg
    57961 tttcattcac cacaagtggc gtattgatcg taaaactctg ccctgcctgt ttgagctgaa
    58021 tgcctaaacc agtgatattg ctgcttactg cagcattatc aaaatcgctt ggtgtgccac
    58081 tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc cgtatcgcag gtaatggaat
    58141 acggcacggt ttgctcataa ttgctgccat taatgtcatc aataagcaca tcctgaaaag
    58201 ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg cggctgcact aaataaccgt
    58261 gaaaagagac gtttgagagt gccatcgagc aaaagcccca cagcaaaata agcgagatcc
    58321 gtttcattgg taatccacca caattgtggc actggcgtta aattcagctg ccgctaacgt
    58381 tgtaccactc tgtttcaccg gcacagcttc cagaacggga actcccgagg aaaaattaaa
    58441 tggcaaccat gagccagagg ttaagtcgag tattgtgtga tcactacttt tctgaacccg
    58501 aataccaaac ccaggaatac ccgtgctgat aactgtttca ccgttaatca cgaccgttgt
    58561 cgcctgcatc tgtagacgca ggtcatccat tgccagattg ttacaaacaa gcgagtagtc
    58621 aatgggacgt ttataatcaa ccctattgat cttattaatg aatacattac caaactcaac
    58681 tgccgcacca tttaccgtac aaggaggagg ggcatcgaca agcacccgta aattcatact
    58741 tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca caacatatca gtaacccagt
    58801 taccgcccag acagaacaag atcctttcat actggctctc ccggcctcag cctttgcggc
    58861 tctgctcttc atcgacctag caactgttac cgttgcaagt gaaaaagagc ggcatccgag
    58921 cgccatagtc gttgacatag gtcagtaccg gcacgctacc catcttcaca ttcagcggtg
    58981 cagaggtttt cggcgacatc actattggcg agaaaccggc tgccggattg ccatttttct
    59041 gtgtgctggc attactgaaa atgacgtaat acgacgtcgg gttattgacg gtaaattcct
    59101 gaccgttgcg ggttaaagta atcttatatt gctaggggtt tttcatgctg acgttctcca
    59161 gcgccttcgg tcgccagaac aacttaatac gcgtttgtag tgctatttgc agcgtgttgg
    59221 ctttgtttga ttttggcggg atctcacgca cgttgaagta gaacaggctt tcacgatcgg
    59281 caggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc atcattgaat
    59341 cgatgcgttg caccggtggc agaacagtta acggtgaact gatcttgtta cccttttcat
    59401 cttcgatcca gctttgggcc agataaggca gtttcggatc gttattgcgc aacgtcacgc
    59461 tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt acagaagcct
    59521 gggcagacaa actggcagcg ctaagcagta acgctagcgt cgtagcaccc aatttggcag
    59581 agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc tgaaacagaa
    59641 acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg ctttatgtca
    59701 tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa gccgttaaac
    59761 agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc actccagaac
    59821 acactcatgt gttcgccagg tttcactcca gccagataaa cattgccgtc atcatcgaca
    59881 aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg atggctgccg
    59941 tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa tttgcggtag
    60001 ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc ggcgttttcc
    60061 ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac gtcagaaacg
    60121 acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac cggaacatcg
    60181 gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattctgggt acggtgaagt
    60241 gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc agaggtgtat
    60301 tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc atggctgtaa
    60361 tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg
    60421 tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc atagtaccgt
    60481 tatagctaac ggtactgttg tcgccccacg gcatactgag cgaaatgtac atgcctttgt
    60541 cggcctggtt gtcatactcg taacggtagc cagtcatcga gatgctgaca ttacgaatac
    60601 tgcccatatt gaaatagtgc gagagcatga tgttgtagtt tgtctgctcc tcgcgatccc
    60661 agtaggtatg gcgggtatag ttgagataaa ccgaaacacc cgcatcgcgg aagttctggt
    60721 tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg taccattccg ctgtcactgg
    60781 catccagata ctcgctcatg gtcataaagt tctcttccga gaagcgatat ccagcaaaag
    60841 taacgcggct gttgagctgg tcaaagtctt tggaataact cacacggaag gagttaccgt
    60901 ccagcgaacc tttgccataa gcggtgtctt tatccagttt ggtgtgcgag tgagtaacat
    60961 caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac accaagcgcc gcagactgat
    61021 agttttcatc tcccagtgcg ccgccatata acgaccagcc gttagcgatc ccccaggaag
    61081 cttcagcacc agaaaaaaat tcaccctcga catggtgtcc ccactcttgc ggacggccca
    61141 tcatgatctt atagcgaacc tgacctggac gagtgaggta tggcatcgag gcggtgctga
    61201 tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg aatatgcaac gtaccggaga
    61261 cagaatcacc aagatcctga atacgaaacg gtccagccgg aacctgcgtt tcgtaaatca
    61321 cacgccccat ctggctgacg gtcacttttg ctgtggtgtg tgccacgccg gaaatgtctg
    61381 gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc agtactgaca ctgccaccaa
    61441 catagttaaa accatcaaaa atatcggatc tgaggtaatc ctcgcccagc gccagtttgg
    61501 cttttaatga tggtaacgcc cgcctggcat agtagcgact ccactcccat ttttttgagt
    61561 ttcatcgccg ctgaattcat cgtcatcatt actgcgagta tgttgatagt tagtctgcca
    61621 gtcagcacgc atacgccacg gccctaggtt aaccccaacc gtcccgttgc cgctgatctc
    61681 gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc
    61741 gacgatcccg gagatgccgt catcccaacg tgaaggcgga tccctgtcgg gataagtata
    61801 ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat
    61861 ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt
    61921 tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata
    61981 gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg
    62041 ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga
    62101 aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg
    62161 aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg caatgcacca
    62221 cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta
    62281 ttcttgttcg gtcaaaatac atttaaatcc cccatagcag tgtcgctatg ggggtatgcc
    62341 ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa ttacccagat ctggcgcatc
    62401 agctgcgccg accagccagg cgttaaagtt cagtgtctgc ttcgctttgc ctttgttggt
    62461 agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag
    62521 agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat
    62581 ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt
    62641 ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
//
LOCUS       DAJTYL010000009        61614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYL010000009 DAJTYL010000000
VERSION     DAJTYL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.009"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..332)
                     /gene="folK"
                     /locus_tag="OO572_000566"
     CDS             complement(<1..332)
                     /gene="folK"
                     /locus_tag="OO572_000566"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6659683.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTER"
     gene            complement(329..1747)
                     /gene="pcnB"
                     /locus_tag="OO572_000567"
     CDS             complement(329..1747)
                     /gene="pcnB"
                     /locus_tag="OO572_000567"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6659684.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1786..2712)
                     /gene="gluQRS"
                     /locus_tag="OO572_000568"
     CDS             complement(1786..2712)
                     /gene="gluQRS"
                     /locus_tag="OO572_000568"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6659685.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2749..3204)
                     /gene="dksA"
                     /locus_tag="OO572_000569"
     CDS             complement(2749..3204)
                     /gene="dksA"
                     /locus_tag="OO572_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6659686.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3382..4086)
                     /gene="sfsA"
                     /locus_tag="OO572_000570"
     CDS             complement(3382..4086)
                     /gene="sfsA"
                     /locus_tag="OO572_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6659687.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4101..4631)
                     /gene="thpR"
                     /locus_tag="OO572_000571"
     CDS             complement(4101..4631)
                     /gene="thpR"
                     /locus_tag="OO572_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6659688.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4705..7134
                     /gene="hrpB"
                     /locus_tag="OO572_000572"
     CDS             4705..7134
                     /gene="hrpB"
                     /locus_tag="OO572_000572"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6659689.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7330..9864
                     /gene="mrcB"
                     /locus_tag="OO572_000573"
     CDS             7330..9864
                     /gene="mrcB"
                     /locus_tag="OO572_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6659690.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10084..12327
                     /gene="fhuA"
                     /locus_tag="OO572_000574"
     CDS             10084..12327
                     /gene="fhuA"
                     /locus_tag="OO572_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6659691.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12378..13175
                     /gene="fhuC"
                     /locus_tag="OO572_000575"
     CDS             12378..13175
                     /gene="fhuC"
                     /locus_tag="OO572_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6659692.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13175..14065
                     /gene="fhuD"
                     /locus_tag="OO572_000576"
     CDS             13175..14065
                     /gene="fhuD"
                     /locus_tag="OO572_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6659693.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14062..16044
                     /gene="fhuB"
                     /locus_tag="OO572_000577"
     CDS             14062..16044
                     /gene="fhuB"
                     /locus_tag="OO572_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6659694.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16079..17359)
                     /gene="hemL"
                     /locus_tag="OO572_000578"
     CDS             complement(16079..17359)
                     /gene="hemL"
                     /locus_tag="OO572_000578"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6659695.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17584..19005
                     /gene="clcA"
                     /locus_tag="OO572_000579"
     CDS             17584..19005
                     /gene="clcA"
                     /locus_tag="OO572_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6659696.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19029..19094
                     /locus_tag="OO572_000580"
     CDS             19029..19094
                     /locus_tag="OO572_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659697.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19087..19431
                     /gene="erpA"
                     /locus_tag="OO572_000581"
     CDS             19087..19431
                     /gene="erpA"
                     /locus_tag="OO572_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6659698.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19478..20101)
                     /locus_tag="OO572_000582"
     CDS             complement(19478..20101)
                     /locus_tag="OO572_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6659699.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20139..20939)
                     /gene="btuF"
                     /locus_tag="OO572_000583"
     CDS             complement(20139..20939)
                     /gene="btuF"
                     /locus_tag="OO572_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6659700.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20932..21630)
                     /gene="mtnN"
                     /locus_tag="OO572_000584"
     CDS             complement(20932..21630)
                     /gene="mtnN"
                     /locus_tag="OO572_000584"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6659701.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21714..23231
                     /gene="dgt"
                     /locus_tag="OO572_000585"
     CDS             21714..23231
                     /gene="dgt"
                     /locus_tag="OO572_000585"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6659702.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23361..24785
                     /gene="degP"
                     /locus_tag="OO572_000586"
     CDS             23361..24785
                     /gene="degP"
                     /locus_tag="OO572_000586"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6659703.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24940..26096
                     /gene="cdaR"
                     /locus_tag="OO572_000587"
                     /pseudo
     CDS             24940..26096
                     /gene="cdaR"
                     /locus_tag="OO572_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26185..26571)
                     /locus_tag="OO572_000588"
     CDS             complement(26185..26571)
                     /locus_tag="OO572_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6659704.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26885..27709)
                     /gene="dapD"
                     /locus_tag="OO572_000589"
     CDS             complement(26885..27709)
                     /gene="dapD"
                     /locus_tag="OO572_000589"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6659705.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27740..30412)
                     /gene="glnD"
                     /locus_tag="OO572_000590"
     CDS             complement(27740..30412)
                     /gene="glnD"
                     /locus_tag="OO572_000590"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6659706.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30474..31268)
                     /gene="map"
                     /locus_tag="OO572_000591"
     CDS             complement(30474..31268)
                     /gene="map"
                     /locus_tag="OO572_000591"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6659707.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31636..32361
                     /gene="rpsB"
                     /locus_tag="OO572_000592"
     CDS             31636..32361
                     /gene="rpsB"
                     /locus_tag="OO572_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6659708.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32619..33470
                     /gene="tsf"
                     /locus_tag="OO572_000593"
     CDS             32619..33470
                     /gene="tsf"
                     /locus_tag="OO572_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6659709.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33617..34342
                     /gene="pyrH"
                     /locus_tag="OO572_000594"
     CDS             33617..34342
                     /gene="pyrH"
                     /locus_tag="OO572_000594"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6659710.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34634..35191
                     /gene="frr"
                     /locus_tag="OO572_000595"
     CDS             34634..35191
                     /gene="frr"
                     /locus_tag="OO572_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6659711.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35283..36479
                     /gene="ispC"
                     /locus_tag="OO572_000596"
     CDS             35283..36479
                     /gene="ispC"
                     /locus_tag="OO572_000596"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6659712.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36668..37426
                     /gene="ispU"
                     /locus_tag="OO572_000597"
                     /gene_synonym="uppS"
     CDS             36668..37426
                     /gene="ispU"
                     /locus_tag="OO572_000597"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6659713.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37439..38296
                     /gene="cdsA"
                     /locus_tag="OO572_000598"
     CDS             37439..38296
                     /gene="cdsA"
                     /locus_tag="OO572_000598"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6659714.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38308..39660
                     /gene="rseP"
                     /locus_tag="OO572_000599"
     CDS             38308..39660
                     /gene="rseP"
                     /locus_tag="OO572_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6659715.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39690..42122
                     /gene="bamA"
                     /locus_tag="OO572_000600"
     CDS             39690..42122
                     /gene="bamA"
                     /locus_tag="OO572_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6659716.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42244..42729
                     /gene="skp"
                     /locus_tag="OO572_000601"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42244..42729
                     /gene="skp"
                     /locus_tag="OO572_000601"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6659717.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42733..43758
                     /gene="lpxD"
                     /locus_tag="OO572_000602"
     CDS             42733..43758
                     /gene="lpxD"
                     /locus_tag="OO572_000602"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6659718.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43863..44318
                     /gene="fabZ"
                     /locus_tag="OO572_000603"
     CDS             43863..44318
                     /gene="fabZ"
                     /locus_tag="OO572_000603"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6659719.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44322..45110
                     /gene="lpxA"
                     /locus_tag="OO572_000604"
     CDS             44322..45110
                     /gene="lpxA"
                     /locus_tag="OO572_000604"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6659720.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45110..46258
                     /gene="lpxB"
                     /locus_tag="OO572_000605"
     CDS             45110..46258
                     /gene="lpxB"
                     /locus_tag="OO572_000605"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6659721.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46255..46851
                     /gene="rnhB"
                     /locus_tag="OO572_000606"
     CDS             46255..46851
                     /gene="rnhB"
                     /locus_tag="OO572_000606"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6659722.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46888..50370
                     /gene="dnaE"
                     /locus_tag="OO572_000607"
     CDS             46888..50370
                     /gene="dnaE"
                     /locus_tag="OO572_000607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6659723.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50383..51342
                     /gene="accA"
                     /locus_tag="OO572_000608"
     CDS             50383..51342
                     /gene="accA"
                     /locus_tag="OO572_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6659724.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51441..53582
                     /gene="ldcC"
                     /locus_tag="OO572_000609"
     CDS             51441..53582
                     /gene="ldcC"
                     /locus_tag="OO572_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6659725.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53639..54028
                     /locus_tag="OO572_000610"
     CDS             53639..54028
                     /locus_tag="OO572_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6659726.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54093..55391
                     /gene="tilS"
                     /locus_tag="OO572_000611"
     CDS             54093..55391
                     /gene="tilS"
                     /locus_tag="OO572_000611"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6659727.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55440..55700)
                     /gene="rof"
                     /locus_tag="OO572_000612"
     CDS             complement(55440..55700)
                     /gene="rof"
                     /locus_tag="OO572_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6659728.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55687..55887)
                     /locus_tag="OO572_000613"
     CDS             complement(55687..55887)
                     /locus_tag="OO572_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6659729.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56053..56598
                     /locus_tag="OO572_000614"
     CDS             56053..56598
                     /locus_tag="OO572_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6659730.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56595..57017
                     /gene="arfB"
                     /locus_tag="OO572_000615"
     CDS             56595..57017
                     /gene="arfB"
                     /locus_tag="OO572_000615"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6659731.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57031..57741
                     /gene="nlpE"
                     /locus_tag="OO572_000616"
                     /gene_synonym="cutF"
     CDS             57031..57741
                     /gene="nlpE"
                     /locus_tag="OO572_000616"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6659732.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57896..58720)
                     /locus_tag="OO572_000617"
     CDS             complement(57896..58720)
                     /locus_tag="OO572_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6659733.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58773..60491)
                     /gene="proS"
                     /locus_tag="OO572_000618"
     CDS             complement(58773..60491)
                     /gene="proS"
                     /locus_tag="OO572_000618"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6659734.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60602..61309)
                     /gene="tsaA"
                     /locus_tag="OO572_000619"
     CDS             complement(60602..61309)
                     /gene="tsaA"
                     /locus_tag="OO572_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6659735.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61306..>61614)
                     /gene="rcsF"
                     /locus_tag="OO572_000620"
     CDS             complement(61306..>61614)
                     /gene="rcsF"
                     /locus_tag="OO572_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6659736.1"
                     /translation="QPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQA
                     SNQDSPPSIPTARKQMQINASKMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK
                     "
BASE COUNT        14449 a        15284 c        17118 g        14763 t
ORIGIN      
        1 cgttcggtat ttatcacttc attaccaaac agcatgatat cgagatccag cgtgcgtggt
       61 ccccagcgtt cagctttacg aacacgaccc tgttgcagtt ctatgcgctg tgtatggttg
      121 agtaactctt ccggggcggg cgaagtttca agcgccacgg cggcgttgag gtaatcgggt
      181 tgatcttgcg gccccagcgg tggggtgcgg taaaacgaag aaacggcaag aatgcggctt
      241 tcagggatat cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga
      301 ttgctgccta tggcaatata cgccactgtc atgtggtacc ctcacgacgc ggtgcgcgtt
      361 tgcgtggacg acgagtacga cgacgcggtg acggttcttc atccagctcg ttaagcatcc
      421 ctttttgatc tggtggtgcg gaaacctgga actcacccca ccatttcacc agacgctgca
      481 gttcagcatt acgttcaact tcagctcgca aggccaacag gtcataagcc gcacggaact
      541 taggatgctc cagcagtttc catgcgcgtt taccctgacg acgggacata cgcaactgca
      601 actgccagat atcgcgggtt aacgtcgtca gacgtttcgg gattgccagt gaacggcagg
      661 cttcgtccag cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt
      721 cctgggcgat cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacaaga
      781 acgccgggtt cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt
      841 caatgatccg ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga
      901 acagcggctg gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg
      961 cttgtagcag tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg
     1021 tagcgaggcg agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg
     1081 caaaacgtac cgcgcgcagc atacgtaccg gatcttcacg gtagcgcgtt tccgggttac
     1141 caatcagacg gataacgccg tccttcagat ccttcatacc accaacgtaa tcacggacgg
     1201 taaaatccgc tacgctgtaa tacaggctgt tgatagtgaa atcgcggcgc tgggcgtctt
     1261 cttcgatgga gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg
     1321 tcgtgcggtc gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct
     1381 ccgggccaaa cattacatga gccagacgga aacggcgacc caccaggcgg cagttacgga
     1441 acagtttgcg cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt
     1501 tgccaagtaa caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttgt
     1561 tgagcctgta cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct
     1621 gctcacgcgg gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct
     1681 cgcggcttag caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta
     1741 gtgtcaaaca tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt
     1801 tgagaatgtt gaatttacaa ttgccgactc gggcacggcg gtcagcatcc agtttttgac
     1861 ggctgactga aggatttgct cgacgctgaa atcctgccag tgtgcttctg cctgctgccc
     1921 cagaaattga agtgccgcga ttagtaccgg gcgtggatcg cctttcggca acgcaggcgc
     1981 atgattctgc ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta
     2041 atctggcact ttccagccaa aaagctggta cagcgggatt tgccttactg ttggttcaat
     2101 cagatcagcc ccacgcacta tttctgtaac gccctggaaa tgatcatcaa ccacaacagc
     2161 caggttgtag gcgaacaacc catcacggcg atgaatgata aaatcttccc gtgccagttt
     2221 ttcgtcggcg tgaataatgc ctcgcagcag gtcagtaaat tgcgtgaccg gatgctgctg
     2281 gcggatacgc actgcggcgt tgtctggtcc atgatgcaac acccggcaat gaccgtcgta
     2341 aataccgcca atgctttgaa tacgcgcacg cgtacaggtg cagtaataac ttagtccttg
     2401 ttcatgtaac caggcgagtg cttcacgata ggcgtcgtga cgttgcgatt gccagagaac
     2461 atcgccgtcc cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc
     2521 gggaacttca cgaggcgggt cgatatcttc tatgcgtacc agccagcgac cttgccgggc
     2581 gcgagcctgc aaatagctgc cgagcgcggc gatcagagag ccaaaatgaa gctcgccgga
     2641 aggagagggg gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata
     2701 aggcgggagc atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat
     2761 ctgtttttcg cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg
     2821 cgcttccaga cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc
     2881 gtcttccact tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg
     2941 gttacgcagt tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa
     3001 gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt
     3061 acgccatgct tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc
     3121 gcccggcttc tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt
     3181 tttacggttt tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg
     3241 ttcgggggaa aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta
     3301 aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa
     3361 attaaccagt tacttacttt actacaatgt aaccggcagt gattttttaa gagccatgcc
     3421 ttcagcagaa atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga
     3481 caatagttgc gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga
     3541 atgcagcacg gcgaaaaaaa taaccgcacg ctggccttca gccgctacgc tcatcaactc
     3601 ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc
     3661 cgctaacgta accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat
     3721 aaagtcaata cggctgcgtt cggagccgta ttttacttcg cttttcagcg agctatagcc
     3781 tgacagttct gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag
     3841 cgtattgacg caaataaatg cgccgctctg gctttgagtt aattcccagg tgtgtgggta
     3901 tttccgtttg gtgttgtctg aagtcgaata ccagacggta tcgccaggcg ttgcacaacc
     3961 ggtcatcgca cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac
     4021 atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa
     4081 ttccattgcg acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt
     4141 gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc ataagaccag
     4201 ttaaaacccg gcggcgggaa tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt
     4261 ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata
     4321 ttcgccagct ggattaagcc gcgtggaggc tgacgcatcc ctaaccacac cacacgcgaa
     4381 cgcagccatt gtccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg
     4441 gctaaaagag aaagcgcctt ctctttctct gcgctcactt cgcctaaaaa tgccagagtc
     4501 aggtgcaaat tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc
     4561 caatggataa tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc
     4621 ggttcagaca tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag
     4681 aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgaa
     4741 ttacttaccg ctctcgattg tgcgccgcag gtattattaa gtgcgccgac cggggccggg
     4801 aaatcaacct ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc
     4861 ctgctggagc cgcgtcgtct ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt
     4921 aacgaaaagc caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggctg
     4981 aatacccgcc tggaagtggt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg
     5041 gaactgagcg gtgttggatt ggtgatcctt gatgagtttc atgagcgcag tttgcaggcg
     5101 gatttggcgt tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg
     5161 ctgattatgt cggctacgct ggacaatgac cgcttgcagc aaatgctgcc ggaagcgccc
     5221 gtcgtcatct cagaagggcg ctcgtttccg gttgaacgcc gttatttacc gctgcccgcg
     5281 catcagcgtt ttgacgaagc cgttgcggta gccaccgccg aaatggtgcg tcaggaaagt
     5341 ggatcattac tgttattttt acctggcgtc ggagaaattc agcgcgtgca ggaacaactg
     5401 gcttcgcgca tcggcagtga tgtggtgctc tgcccgctgt atggcgtgtt gtcactgaac
     5461 gatcagcgaa aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc
     5521 aatattgctg aaaccagttt aaccatcgaa ggtattcgtc tggtggtgga ttgtgcccag
     5581 gagcgtgtgg cgcgctttga tccgcgcacg gggcttacgc ggttgattac tcaacgcgtt
     5641 agtcaggcat ccatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatcagc
     5701 ctgcatttaa tcgccaaaga acaagcagaa cgcgccgcgg cgcaaagtga gccgaagatc
     5761 ttacaaagcg atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg
     5821 gcgcagatga gctggctgga tcaaccgcca acggtgaatc tactggccgc gaaacgcctg
     5881 ttacaaatgc tgggggcgct ggagggtgaa cggcttagtg cgcaagggca aaaaatggca
     5941 gcgctgggta acgatccgcg tttagcggca atgctggtta acgcaaagag cgatgacgaa
     6001 gctgctaccg cggcaaaaat tgccgccatt ctcgaagagc cgccacggat gggcaatagt
     6061 gacctgggcg tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg
     6121 ttaaaacgct taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt
     6181 gccggggcgt ttgccgatcg cattgctcat cgccgtgggc aagatggacg ctatcaactg
     6241 gcaaacagca tgggagcgat gctcgatgcc gacgacgcgc taagccgcca cgaatggttg
     6301 atcgcaccgt tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgcta
     6361 ccggtcgata ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc
     6421 gtggaatggg atgacgcgca aggtacgctg aaagcctggc gtcggctaca aatcggtcag
     6481 ttgacggtga aagtgcagcc gctggcgaaa ccgtcagaag acgagttgca tcaggcgatg
     6541 cttaatggca tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag
     6601 ctacgcttgc gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt
     6661 gatgatgaaa gtttgttggc gacgctggaa acgtggctgc tgccacatat gaccggcgta
     6721 cattcactac gcggcctgaa atcactcgac atttatcagg cactacgcgg attacttgat
     6781 tggggaatgc agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga
     6841 agccggatcg ccattcgtta tcatgaagat aacccgcccg cgctggcggt gagaatgcag
     6901 gagatgtttg gtgaggccac taatccgacg atcgcccagg ggcgcgtgcc gctggtgctg
     6961 aagttgcttt cccctgccca aaggccgctg caaatcacgc gtgatttggg ggccttctgg
     7021 aaaggggcgt atcgtgaggt gcaaaaggag atgaaagggc gttatcccaa acacgtctgg
     7081 ccggacgacc cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg
     7141 ttttctccct ctccctgtgg gagagggccg gggtgagggc atcagcgcgc acgttcaccc
     7201 tcaccctaac cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata
     7261 tcttcttctg tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
     7321 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
     7381 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
     7441 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
     7501 aagcctcgtg gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc
     7561 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc
     7621 aaggtctggc aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg
     7681 accatcagca agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg
     7741 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
     7801 ccgtttgatt tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt
     7861 gatcatctgg cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
     7921 gatccgcgtc tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg
     7981 cgcagtggtt tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt
     8041 tacgagcatg atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc
     8101 gcaggtcgca cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc
     8161 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg
     8221 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
     8281 cagagcggcg acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc
     8341 ccggtagaag agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg
     8401 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg
     8461 cgtctgctgc aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt
     8521 ccgctggggg ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg
     8581 gtgcgtcagg agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag
     8641 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
     8701 attccggcac tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc
     8761 gaccgcttta gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc
     8821 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat
     8881 ctgacggctt taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca
     8941 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
     9001 agcgaaagcg gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg
     9061 gtaaatctgg ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc
     9121 gtaccgaaag atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg
     9181 ccaatcgaag tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt
     9241 tctgcactgc gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag
     9301 gcagaacgcg ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
     9361 gtacaacgtg gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg
     9421 aaaacaggga ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg
     9481 gtgaccatca cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc
     9541 ggggcaatgt cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt
     9601 gttccgccag aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
     9661 ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc
     9721 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
     9781 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
     9841 aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat
     9901 ccggtaaaat aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta
     9961 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
    10021 tcttatcttt ataataatca ttctcgttta cgttattatt cactttacat cagagatata
    10081 ccaatggcgc gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta
    10141 gtagccacag cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa
    10201 gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca
    10261 actattgcgg cgcggcagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg
    10321 ccacagtcta tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta
    10381 aaagaagcgc ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc
    10441 tatgaccacc tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg
    10501 aatggcctga agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg
    10561 gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc
    10621 ggtctgttga atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt
    10681 aaagccggta ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat
    10741 gacggtgttt actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa
    10801 gggtcagaag agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa
    10861 accaatttca ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg
    10921 ttgccgaaag agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt
    10981 aatgaagggg cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc
    11041 gatcacgaat ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa
    11101 acctcgcaaa acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc
    11161 aaacagtgtg cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc
    11221 gttgatgatg agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc
    11281 actggcgata tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac
    11341 atcaacgcct ggtttggtta cgacgactct gtgccactgt tcaatctgta caatccggtg
    11401 aatcccgatt tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg
    11461 aataaacaga aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg
    11521 gtcaccctag gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg
    11581 acgaccgata aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt
    11641 gataatggtg taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt
    11701 gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa
    11761 tatgtaccgg aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc
    11821 aacaacctga tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc
    11881 gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt
    11941 tcttatactt acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct
    12001 gcacaggtgc caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg
    12061 ctttcaggtc tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg
    12121 gctaactcct ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg
    12181 gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt
    12241 gaatacgtcg ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc
    12301 gttgcaaccg caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat
    12361 ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc
    12421 gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc
    12481 ctgccgggaa agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca
    12541 aaatgcttgg tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc
    12601 tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc
    12661 ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg
    12721 gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg
    12781 ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc
    12841 gggcgtggat cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga
    12901 cttcagcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc
    12961 aggaacgtgg cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact
    13021 gtgattatct ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa
    13081 ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc
    13141 cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg
    13201 ccgtcgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca
    13261 cgcggcggct attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact
    13321 gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt
    13381 cagcgaacca ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct
    13441 tgaactgctg accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc
    13501 atcagaaatg ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca
    13561 tccgttggcg atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag
    13621 cgcagcggaa acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt
    13681 tgtgaagcgt ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct
    13741 ggtcttcggt ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc
    13801 ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc
    13861 gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct
    13921 aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt
    13981 acccgcagtc tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga
    14041 taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg
    14101 gcgctgttag tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg
    14161 cgcagtcagt gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt
    14221 tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg
    14281 ctggtgggcg tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg
    14341 ggcgttgcaa caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc
    14401 gcgatggcga gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt
    14461 ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg
    14521 agcctttatt gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa
    14581 agcatgtttc tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt
    14641 ttatggccgc agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc
    14701 ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt
    14761 ctggcggcgc tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt
    14821 atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg
    14881 ctgccacgac tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa
    14941 atcatcctct ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg
    15001 atcggtgcgc cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat
    15061 atgaaggtta acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg
    15121 ggcggcgtgc tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac
    15181 ggctggacgt gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga
    15241 attatggcgg cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga
    15301 ctgaccggca acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt
    15361 ggtgtggtgt taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca
    15421 ggcagtctcg gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga
    15481 ttttccccac accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt
    15541 ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc
    15601 ggttcgacct acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt
    15661 ttgctggcga ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat
    15721 accgcgcggg cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg
    15781 gcttgcctga cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg
    15841 gcaccgcata ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg
    15901 gcgctggtgg gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt
    15961 ccattccaga tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat
    16021 ttgctgagaa agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc
    16081 acaacttcgc gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt
    16141 gcgccacaga cataaagcct gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca
    16201 gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc
    16261 acgtcacgga ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga
    16321 ccagcggaat tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca
    16381 gctcatccag cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg
    16441 ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg
    16501 ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga
    16561 tgatttttcc gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta
    16621 gcgctacgcg gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc
    16681 acagcgcacg cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg
    16741 ccaccggctc gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta
    16801 cagaggccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc
    16861 ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt
    16921 cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac
    16981 gggccaggcg gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat
    17041 ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg
    17101 caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt
    17161 tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa
    17221 catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag
    17281 taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa
    17341 gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt
    17401 ccatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa
    17461 tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat
    17521 tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat
    17581 tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga
    17641 caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg
    17701 gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg
    17761 ttgcagaacc aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta
    17821 accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa
    17881 tacgcgcccg aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa
    17941 cgtcctgttc gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc
    18001 ggcggaggca tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt
    18061 cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca
    18121 accggtgctg ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt
    18181 attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt
    18241 attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt
    18301 gacgtcggta aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt
    18361 attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg
    18421 ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggggg ggcgattggc
    18481 ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg
    18541 attcctatcg ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct
    18601 cgggtcatta ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg
    18661 atgctggcgc tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt
    18721 ccgcaatatc acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg
    18781 gcatctattc gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac
    18841 cagctcattt tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc
    18901 ggcgggaaac ctctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag
    18961 caactggcgc gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt
    19021 attagataat ggcgattatt attgggttag aatttgcccg attgccgatg tcgtttggag
    19081 caaaatatga gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa
    19141 gttaaaagcc tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc
    19201 ggtggcggtt gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc
    19261 gatatgacca tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat
    19321 ctggtcggcg gttccgttga ctacaccgaa ggactggaag gttctcgttt catcgtgacc
    19381 aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt
    19441 catgtgccgg ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg
    19501 caaacgtcgg tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga
    19561 ccatgcccat catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt
    19621 ggacaatacc gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga
    19681 tttcgcggac cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac
    19741 aaaccgcgat taacggaccg gcttccgcat taaaggcttt attcacgcta atgccgacaa
    19801 acaccgccag accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct
    19861 ggcgcaccag cacgatggtc agcatgctgg tgaccatggc aacgaccaga tcggtgggat
    19921 ctttcaccca aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgtcgccta
    19981 ctgcggtaac cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta
    20041 acaaaacgcc gtagatggca aataccgctg tgccgactat atccagccaa tagacgagca
    20101 ttgttcaatc cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca
    20161 ttacagagct gttgtgctgc gaggataata cgtgggcttg cacgttcaaa ccagtcactc
    20221 gtgagaggaa taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt
    20281 tggtccggtc cgcctgtaat gacaatagcc tgtggcgagc gtgctaacac ctgttcgcgg
    20341 ctaacttgcg gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg
    20401 agtacctggt tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc
    20461 agaaaaacac gttttttagg tttatcagca tattgcgctt tcaattgcac gtactgatcc
    20521 agcagggatt gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga
    20581 cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc
    20641 agcgaagcca gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga
    20701 tcgggtttca gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca
    20761 atcttttgcg cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg
    20821 gcggcaaagg caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg
    20881 agccacagtg gcgcgagaaa agacagggcg accagcgccc tgaacagtga tttagccatg
    20941 tgccagtttc tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag
    21001 gaactcatcg aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac
    21061 aacaaacggg acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac
    21121 agcaatggcc tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa
    21181 agcgtcgccg ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc
    21241 ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg
    21301 accgtattca taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac
    21361 aacgatatcg cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat
    21421 cacatctggc ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc
    21481 gatgcccgat ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca
    21541 accgccgaga ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc
    21601 ttccattgca ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag
    21661 gcatagttta ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac
    21721 agattgattt ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta
    21781 aaaccgaaca tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc
    21841 cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg
    21901 tgcgcacgcg tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag
    21961 aaattttaag ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga
    22021 ccggcccctt tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc
    22081 cgtttggtca ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg
    22141 aagatgccga aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac
    22201 gggacgggga agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt
    22261 ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt
    22321 gggcacaggt tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc
    22381 ctgagacaca tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata
    22441 ttgcccgctt gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga
    22501 ttatggaagc tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga
    22561 aaagaatatt taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg
    22621 agaaaggttc gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata
    22681 gtttaagccg cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata
    22741 aactggtacc ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa
    22801 cgtttaatca tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata
    22861 aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt cgagcagctt gaattgcagg
    22921 gctatcgggt cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat
    22981 cagactttac tgaactggta gaaaaagaac gggtgaaacg tttccctatt gagtcgcgct
    23041 tattccacaa actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac
    23101 cgtcagattc tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg
    23161 attatatcag cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg
    23221 tagaacaata accagtcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact
    23281 ttagttcgga acttcgggct ataaaacgaa tctgaagaac acagcaattt tgcgttatct
    23341 gttaatcgag actgaaatac atgaaaaaaa acacattagc actgagtgca ctggctctga
    23401 gtttaggttt ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga
    23461 cagcccagca gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca
    23521 gcattaacgt agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc
    23581 agttcttcgg tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt
    23641 tctgccaggg gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg
    23701 gttccggcgt catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg
    23761 ataacgcgac ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg
    23821 ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat ccagaacccg aaaaaactga
    23881 ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg
    23941 gtaacccgtt tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta
    24001 gcggcctgaa tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg
    24061 gtaactccgg tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga
    24121 tcctcgcacc ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga
    24181 aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta
    24241 tggggactga gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg
    24301 ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg
    24361 atgtgatcac ctcactgaac ggtaagccga tcagcagctt tgccgcactg cgtgctcagg
    24421 tgggtactat gccggtgggc agcaaactga ccctgggctt actgcgcgac ggtaagcagg
    24481 ttaacgtgaa cctggaactg cagcagagca gccagaatca ggttgattcc agctccatct
    24541 tcaacggcat tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga
    24601 acaacgtgaa gacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta
    24661 ttggtgcgaa ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca
    24721 aaccgtctgt gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc
    24781 agtaatctcc ctcaacccct tcctgaatac gggaaggggt tctccttaca atctgtgaac
    24841 ttcgccacaa ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta
    24901 cgtacttcct tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat
    24961 accaaaatgg cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac
    25021 gtaatggatg cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg
    25081 cacgaaggtg cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta
    25141 gcacgtcatc tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa
    25201 attgtcggcg taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg
    25261 gtctgcatga cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag
    25321 gatagccgtt tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc
    25381 gcacttctga atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta
    25441 ttgttgaggt cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacaacaac
    25501 tgcaaaacgc gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg
    25561 aaatggtggt gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc
    25621 gtaagcgagt tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg
    25681 tttcactggg caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga
    25741 aaacgacgat ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg
    25801 atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg
    25861 cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg
    25921 cggcgtggtt tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc
    25981 gtaataccct ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca
    26041 attttgatga caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtagatta
    26101 tgcgttaagg ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg
    26161 cccgagttaa ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat
    26221 ctcgctgatc ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg
    26281 cttaagatca acttcgctgc ggtcacgctg acagatttga tcaagctcat caatgatata
    26341 ccgtagattc gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac
    26401 gaccgtttta cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt
    26461 gtctttctgg aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg
    26521 atcaatttct tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc
    26581 tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg cttggcccgc ccggctcgac
    26641 tgtgatcgca gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt
    26701 acctgcttta ttgtggcgat attccatggg atggattagc acatttatct tcgttttgcg
    26761 aattacatga cagtccgaaa caagatgaaa aagtatttaa aaaaggggga ggaagaatga
    26821 gtaggacgaa ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt
    26881 actcttaatc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    26941 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat
    27001 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27061 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt
    27121 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaataa
    27181 tggtcgggtt agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt
    27241 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    27301 cttcatcaac atatgcgccg atgttaacgt aagacggcat cagcacggtg ttacgggcaa
    27361 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    27421 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    27481 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    27541 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca
    27601 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct
    27661 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    27721 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    27781 gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga
    27841 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    27901 gatcggcaaa aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt
    27961 cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28021 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28081 gcacctgctc cagaccaaac cgaataacct catggcgatc tgcggacagc gggctgccat
    28141 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28201 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    28261 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    28321 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    28381 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    28441 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc
    28501 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    28561 agagttcgcg caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa
    28621 tgtcagccac tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg
    28681 caaactgctt gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca
    28741 gcaagtgctg gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga
    28801 gttctgcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    28861 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    28921 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    28981 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29041 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29101 ttggcaatag tccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc
    29161 gtgcttgcgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    29221 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    29281 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    29341 cgcgtagctg aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga
    29401 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    29461 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    29521 gctgagcgac gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca
    29581 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    29641 ccgctgaggt taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac
    29701 ggcgggccac ccattgcaga gtgtgaatat cgcgcaagcc gccagggcta cttttgatgt
    29761 ctggttcaag gttgtagctg gtgccatggt aacgctgatg gcgctggttc tgttcttcaa
    29821 ctttcgctgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    29881 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    29941 cggttaaatc cgataaccct tccaacatgc actcttcaag cgtgcgcaca ctatgaccga
    30001 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30061 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30121 cacgaccgta gccaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt
    30181 caatccataa tcgttgcagg agctggtcta tgaactcggt acgtgcctca atcaactgtt
    30241 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    30301 cttttatccc acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac
    30361 cgggcagggt ggagagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    30421 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    30481 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcaaaat ttcgcagccg
    30541 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    30601 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctca
    30661 atggtgaacg tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc
    30721 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    30781 gagaagcctt ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg
    30841 cctggtttta ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacaggcgt
    30901 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    30961 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg
    31021 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31081 tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg
    31141 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31201 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    31261 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    31321 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    31381 aaggttttct ataatagaaa attcgacgtc tgatgctgta cacagcgcca acaattattg
    31441 gtgtccacga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    31501 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    31561 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    31621 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    31681 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    31741 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    31801 gaactgaaca agattgtttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    31861 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    31921 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    31981 gacctggaaa ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg
    32041 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32101 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32161 gcaaacaact tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    32221 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    32281 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    32341 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    32401 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    32461 gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    32521 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    32581 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    32641 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    32701 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    32761 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    32821 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    32881 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    32941 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33001 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33061 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33121 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33181 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    33241 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    33301 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    33361 agcataacgc tgaagtgact ggcttcatcc gtttcgaagt gggtgaaggc atcgagaaag
    33421 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    33481 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    33541 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    33601 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    33661 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    33721 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    33781 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    33841 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ttggcaatgc gtgatgcact
    33901 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    33961 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34021 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34081 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34141 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34201 agagctgaaa gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat
    34261 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    34321 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    34381 gtatcgcggg ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    34441 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    34501 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    34561 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    34621 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    34681 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    34741 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    34801 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    34861 ggccgttgaa aaagcgatta tggcctccga tctcggcctg aacccgaact ctgcgggtag
    34921 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    34981 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa
    35041 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35101 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35161 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    35221 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    35281 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    35341 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    35401 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    35461 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa
    35521 gtgggcaaca agccgcttgc gatatggcag cgcttgagga ggttgatcag gtgatggcag
    35581 ccattgttgg tgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    35641 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    35701 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgctatt tttcagagtt
    35761 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    35821 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    35881 caatgacgcc cgatcaagct tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    35941 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36001 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36061 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36121 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36181 gcaaactaag tgcgttgaca tttgccgcac cggattatga gcgttatcca tgcctgaaac
    36241 tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg
    36301 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    36361 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    36421 ctgttgatgc gaacgcgagt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    36481 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    36541 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    36601 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    36661 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    36721 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    36781 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    36841 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    36901 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    36961 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgtt ttaactcgcg
    37021 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc ggaaataccg gtctgacgct
    37081 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37141 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgttaaacca
    37201 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggagagca
    37261 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    37321 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    37381 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    37441 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    37501 tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg
    37561 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    37621 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    37681 tcaaccgctg gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt
    37741 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    37801 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    37861 gcactatgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    37921 atggggcgct gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    37981 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactcg ctactgcagc
    38041 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38101 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38161 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    38221 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    38281 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    38341 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    38401 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    38461 actgataagc tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa
    38521 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    38581 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    38641 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    38701 ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    38761 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    38821 aaaattggcg atgaaagcac caccattaca gtagcgccat ttgacagcga ccaacggcgg
    38881 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    38941 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca
    39001 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39061 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39121 ttagcgttag aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt
    39181 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    39241 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    39301 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    39361 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    39421 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    39481 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    39541 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    39601 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    39661 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    39721 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    39781 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgtacag
    39841 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    39901 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    39961 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40021 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40081 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40141 ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40201 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    40261 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaat gtggtaggcg
    40321 atcgtaaata ccagaaacag aaactggcgg gcgatcttga aaccctgcgc agctactatc
    40381 tggaccgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    40441 aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg
    40501 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcaactg actaagatcg
    40561 agccgggcga gctgtataac ggcactaaag tgaccaaaat ggaagatgac attaaaaagc
    40621 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    40681 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    40741 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    40801 agatggaagg tgcatggttg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc
    40861 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    40921 aggttgatgt agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg
    40981 gttacggtac tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag
    41041 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41101 tgtcggtaac caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct
    41161 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    41221 cagacgtgac gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt
    41281 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    41341 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    41401 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    41461 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    41521 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    41581 gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    41641 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    41701 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    41761 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    41821 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    41881 actcggttcg tacttcattc ttctgggata tgggtaccgt ttgggataca aactgggatt
    41941 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42001 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42061 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42121 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42181 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    42241 attgtgaaaa agtggttatt agctgcaggt cttggtttag cactggcaac ttctgctcag
    42301 gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa
    42361 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    42421 atggaaaccg atctgcaggc taaaatgaaa aaactgcagt ccatgaaagc gggcagcgat
    42481 cgcaccaaac tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    42541 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    42601 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    42661 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    42721 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    42781 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    42841 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    42901 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    42961 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43021 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43081 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43141 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43201 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    43261 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    43321 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    43381 cgcggcgcgc tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag
    43441 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    43501 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    43561 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    43621 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    43681 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    43741 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    43801 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    43861 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    43921 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    43981 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44041 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44101 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc
    44161 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    44221 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg
    44281 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    44341 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg
    44401 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    44461 acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat cagtttgcct
    44521 ccatcggcga agttaaccag gatttgaaat atgctggcga accgacccgt gtggaaatcg
    44581 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    44641 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    44701 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    44761 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    44821 cgcatgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgttattg
    44881 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    44941 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctaatttat cgtagcggta
    45001 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45061 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgacca
    45121 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45181 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    45241 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    45301 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    45361 gcgttttggc gaactgaagc cagatgtttt tgttggtatt gatgcgcccg acttcaatat
    45421 tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc
    45481 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    45541 cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat
    45601 cggtcatacc atggctgatg ccatgccgtt agatccagat aaaaatggtg cccgtgatgt
    45661 gctggggatc ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga
    45721 agtcgaaatg cttagtgccg atttcttgaa aactgcccag cttttgcgcc agacatatcc
    45781 ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt ttgaacgcat
    45841 caaagctgaa gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc
    45901 gatggtcgcc agcgatgcgg cactactggc gtcggggacg gcagccctgg agtgtatgct
    45961 ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa
    46021 gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt
    46081 caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46141 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46201 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    46261 gaatttgttt atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    46321 ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg
    46381 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa
    46441 gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac
    46501 attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    46561 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    46621 gtgaaagggg atagccgcgt accagaaatc agtgccgcgt ctatcctggc gaaagtgacg
    46681 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat
    46741 aaagggtatc caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat
    46801 catcgtcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    46861 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    46921 cacagcgact actcgatgat cgatggcttg gccaaaaccg caccgctggt aaaaaaggcg
    46981 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg
    47041 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat
    47101 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47161 accggctatc agaatctggc gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    47221 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    47281 tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta
    47341 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    47401 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    47461 cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac
    47521 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    47581 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc
    47641 gacatcccag aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaactgtg
    47701 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    47761 ctggtcaagc gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag
    47821 gaagaacgtc ttaaacgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    47881 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    47941 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48001 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48061 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48121 gatcaggtta tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagattatc
    48181 accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    48241 ccgtacggct ttgtcgaccg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    48301 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    48361 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    48421 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat
    48481 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    48541 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    48601 atcaacaagc ggcgggcgaa gaatggcgag ccaccgctgg atatcgccgc gatcccgctg
    48661 gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    48721 gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg
    48781 atcgcactgg tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt
    48841 atcgaccgta aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa
    48901 agcctgaaat cggtactgga gccaacctac ggcattatct tgtatcagga acaggtcatg
    48961 cagattgccc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgccgtgcg
    49021 atgggtaaga aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca
    49081 gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49141 gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49201 ttatggctga aagcgcacta tccggcggag tttatggcgg cggtaatgac cgccgatatg
    49261 gacaacaccg agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc
    49321 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga tggcgaaatc
    49381 gtgtatggta ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa
    49441 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc
    49501 aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    49561 gggccacacc gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa
    49621 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    49681 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    49741 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    49801 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    49861 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    49921 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    49981 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50041 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50101 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50161 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    50221 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    50281 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    50341 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    50401 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    50461 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    50521 agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg
    50581 gcacgccatc cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    50641 gacgaactgg ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    50701 cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    50761 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    50821 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    50881 tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt
    50941 gaaatgtctc gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51001 ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51061 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg
    51121 gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc
    51181 atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg
    51241 aaagcgcaac tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa
    51301 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    51361 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    51421 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    51481 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    51541 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    51601 gcgtgatttt tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta
    51661 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    51721 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    51781 ccattcgtat gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga
    51841 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    51901 gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga
    51961 atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca
    52021 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52081 atatcgttac caacggaata tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52141 ccggcagtac gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga
    52201 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg
    52261 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc
    52321 aagcacaatg gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca
    52381 acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg
    52441 tgccgtatac tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg
    52501 ttgcgggaaa agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    52561 aggcttcact gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    52621 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttaag acggcggcgg
    52681 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    52741 attttcgcaa agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct
    52801 ggcaaccgcc gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc
    52861 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    52921 tgacaccggg gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg
    52981 tagcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53041 tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53101 cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct
    53161 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    53221 ataagctgat tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc
    53281 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    53341 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    53401 cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    53461 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    53521 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    53581 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    53641 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    53701 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    53761 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt
    53821 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    53881 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    53941 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54001 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54061 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54121 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54181 atcagttggt gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc
    54241 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    54301 agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg
    54361 aggcccaggc gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag
    54421 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac
    54481 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac
    54541 ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc
    54601 atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    54661 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg
    54721 cccgcagcgc cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg
    54781 atttagcaca ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    54841 gtgatgcccg tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc
    54901 cttcccgcga cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt
    54961 ccccctgttt acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55021 ttaaatccgt caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc
    55081 tggaattacc ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc
    55141 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg
    55201 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc
    55261 tacgtgacac cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat
    55321 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa
    55381 cgcttagtta agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat
    55441 caggattcgc ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc
    55501 agacgcagct cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg
    55561 gagactaaat cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    55621 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga
    55681 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    55741 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    55801 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    55861 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    55921 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    55981 tttctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56041 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56101 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56161 caggagcgta tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa
    56221 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg
    56281 ggcattgatt tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc
    56341 acccaggccg cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg
    56401 cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat
    56461 gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    56521 caggatggtg ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc
    56581 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg
    56641 agatcaccgc cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg
    56701 ctattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc
    56761 tgctcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    56821 aataccgcag tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta
    56881 aagatttaac aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag
    56941 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac
    57001 gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg
    57061 gctgtaatca gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt
    57121 tctccggcgc aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg
    57181 tgtgccgatt gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg
    57241 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    57301 tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat
    57361 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag
    57421 ttcaactata cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga
    57481 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    57541 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    57601 gaaaaaccgg tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact
    57661 ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat
    57721 tgcagtagtt tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg
    57781 cgctgcgctt atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta
    57841 ggccggataa ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact
    57901 gagtcagccc aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat
    57961 actgtaactg cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat
    58021 cggcagggct aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg
    58081 catcagtgac aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta
    58141 actgcgcttt cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca
    58201 gtgagcacat ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat
    58261 aaccgctatc tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa
    58321 gatccgggac tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa
    58381 caatctggac gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa
    58441 cgtgactcac agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg
    58501 aggagaacag caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt
    58561 cggtgaaaga gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg
    58621 ctgtggctga tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc
    58681 tggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
    58741 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat
    58801 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg
    58861 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
    58921 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
    58981 ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
    59041 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
    59101 ccgcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
    59161 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
    59221 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    59281 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca
    59341 acttccgggg tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg
    59401 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    59461 ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct
    59521 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    59581 agctcgtgat caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct
    59641 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    59701 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    59761 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    59821 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    59881 ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag
    59941 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    60001 tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    60061 cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    60121 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    60181 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    60241 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg
    60301 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    60361 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    60421 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    60481 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    60541 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    60601 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    60661 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    60721 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    60781 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg
    60841 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    60901 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    60961 cgctgccttt atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag
    61021 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg
    61081 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    61141 aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gctatgagat
    61201 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    61261 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    61321 gttaagcgca gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac
    61381 ttcgcagcta tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg
    61441 cttacgtgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    61501 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    61561 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctg
//
LOCUS       DAJTYL010000010        53190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYL010000010 DAJTYL010000000
VERSION     DAJTYL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.010"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..312)
                     /locus_tag="OO572_000621"
     CDS             complement(1..312)
                     /locus_tag="OO572_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6659737.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            475..571
                     /gene="ffs"
                     /locus_tag="OO572_000622"
     ncRNA           475..571
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO572_000622"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            691..1044
                     /locus_tag="OO572_000623"
     CDS             691..1044
                     /locus_tag="OO572_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6659738.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1086..2636)
                     /gene="pdeB"
                     /locus_tag="OO572_000624"
     CDS             complement(1086..2636)
                     /gene="pdeB"
                     /locus_tag="OO572_000624"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6659739.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2800..3270)
                     /locus_tag="OO572_000625"
     CDS             complement(2800..3270)
                     /locus_tag="OO572_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6659740.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3386..3937)
                     /gene="maa"
                     /locus_tag="OO572_000626"
                     /pseudo
     CDS             complement(3386..3937)
                     /gene="maa"
                     /locus_tag="OO572_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4109..4327)
                     /gene="hha"
                     /locus_tag="OO572_000627"
     CDS             complement(4109..4327)
                     /gene="hha"
                     /locus_tag="OO572_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6659741.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4353..4727)
                     /gene="tomB"
                     /locus_tag="OO572_000628"
     CDS             complement(4353..4727)
                     /gene="tomB"
                     /locus_tag="OO572_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6659742.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5272..8421)
                     /locus_tag="OO572_000629"
     CDS             complement(5272..8421)
                     /locus_tag="OO572_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6659743.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8444..9637)
                     /gene="acrA"
                     /locus_tag="OO572_000630"
     CDS             complement(8444..9637)
                     /gene="acrA"
                     /locus_tag="OO572_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6659744.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9779..10426
                     /gene="acrR"
                     /locus_tag="OO572_000631"
     CDS             9779..10426
                     /gene="acrR"
                     /locus_tag="OO572_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6659745.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10554..13916
                     /gene="mscK"
                     /locus_tag="OO572_000632"
     CDS             10554..13916
                     /gene="mscK"
                     /locus_tag="OO572_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6659746.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14128..14289)
                     /gene="rsmS"
                     /locus_tag="OO572_000633"
     CDS             complement(14128..14289)
                     /gene="rsmS"
                     /locus_tag="OO572_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6659747.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14303..14830)
                     /gene="priC"
                     /locus_tag="OO572_000634"
     CDS             complement(14303..14830)
                     /gene="priC"
                     /locus_tag="OO572_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6659748.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14900..15277
                     /locus_tag="OO572_000635"
     CDS             14900..15277
                     /locus_tag="OO572_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6659749.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15430..15981
                     /gene="apt"
                     /locus_tag="OO572_000636"
     CDS             15430..15981
                     /gene="apt"
                     /locus_tag="OO572_000636"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6659750.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16110..18035
                     /gene="dnaX"
                     /locus_tag="OO572_000637"
     CDS             16110..18035
                     /gene="dnaX"
                     /locus_tag="OO572_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6659751.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17368..17432
                     /gene="dnaX"
                     /locus_tag="OO572_000637"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18088..18417
                     /locus_tag="OO572_000638"
     CDS             18088..18417
                     /locus_tag="OO572_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6659752.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18417..19022
                     /gene="recR"
                     /locus_tag="OO572_000639"
     CDS             18417..19022
                     /gene="recR"
                     /locus_tag="OO572_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6659753.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19132..21006
                     /gene="htpG"
                     /locus_tag="OO572_000640"
     CDS             19132..21006
                     /gene="htpG"
                     /locus_tag="OO572_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6659754.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21187..21831
                     /gene="adk"
                     /locus_tag="OO572_000641"
     CDS             21187..21831
                     /gene="adk"
                     /locus_tag="OO572_000641"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6659755.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21963..22925
                     /gene="hemH"
                     /locus_tag="OO572_000642"
     CDS             21963..22925
                     /gene="hemH"
                     /locus_tag="OO572_000642"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6659756.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22922..23881)
                     /gene="aes"
                     /locus_tag="OO572_000643"
                     /pseudo
     CDS             complement(22922..23881)
                     /gene="aes"
                     /locus_tag="OO572_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24033..25337
                     /gene="gsk"
                     /locus_tag="OO572_000644"
     CDS             24033..25337
                     /gene="gsk"
                     /locus_tag="OO572_000644"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6659757.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25470..27146)
                     /locus_tag="OO572_000645"
     CDS             complement(25470..27146)
                     /locus_tag="OO572_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6659758.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27384..28604)
                     /gene="fsr"
                     /locus_tag="OO572_000646"
     CDS             complement(27384..28604)
                     /gene="fsr"
                     /locus_tag="OO572_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6659759.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28822..30474
                     /gene="ushA"
                     /locus_tag="OO572_000647"
     CDS             28822..30474
                     /gene="ushA"
                     /locus_tag="OO572_000647"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6659760.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30511..30990)
                     /gene="ybaK"
                     /locus_tag="OO572_000648"
     CDS             complement(30511..30990)
                     /gene="ybaK"
                     /locus_tag="OO572_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6659761.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31194..31988)
                     /locus_tag="OO572_000649"
     CDS             complement(31194..31988)
                     /locus_tag="OO572_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6659762.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32126..32467
                     /locus_tag="OO572_000650"
     CDS             32126..32467
                     /locus_tag="OO572_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6659763.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32682..35186)
                     /gene="copA"
                     /locus_tag="OO572_000651"
     CDS             complement(32682..35186)
                     /gene="copA"
                     /locus_tag="OO572_000651"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6659764.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35448..36380
                     /gene="glsA"
                     /locus_tag="OO572_000652"
     CDS             35448..36380
                     /gene="glsA"
                     /locus_tag="OO572_000652"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6659765.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36383..37675
                     /locus_tag="OO572_000653"
     CDS             36383..37675
                     /locus_tag="OO572_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6659766.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37800..38207
                     /gene="cueR"
                     /locus_tag="OO572_000654"
     CDS             37800..38207
                     /gene="cueR"
                     /locus_tag="OO572_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6659767.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38208..38666)
                     /locus_tag="OO572_000655"
     CDS             complement(38208..38666)
                     /locus_tag="OO572_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6659768.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38663..39580)
                     /locus_tag="OO572_000656"
     CDS             complement(38663..39580)
                     /locus_tag="OO572_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6659769.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39726..40403
                     /gene="fetA"
                     /locus_tag="OO572_000657"
     CDS             39726..40403
                     /gene="fetA"
                     /locus_tag="OO572_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6659770.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40390..41169
                     /gene="fetB"
                     /locus_tag="OO572_000658"
     CDS             40390..41169
                     /gene="fetB"
                     /locus_tag="OO572_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6659771.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41232..42086)
                     /gene="cnoX"
                     /locus_tag="OO572_000659"
     CDS             complement(41232..42086)
                     /gene="cnoX"
                     /locus_tag="OO572_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6659772.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42147..42956)
                     /gene="ybbO"
                     /locus_tag="OO572_000660"
     CDS             complement(42147..42956)
                     /gene="ybbO"
                     /locus_tag="OO572_000660"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6659773.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42946..43568)
                     /gene="tesA"
                     /locus_tag="OO572_000661"
                     /pseudo
     CDS             complement(42946..43568)
                     /gene="tesA"
                     /locus_tag="OO572_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43539..44225
                     /locus_tag="OO572_000662"
     CDS             43539..44225
                     /locus_tag="OO572_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6659774.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44222..46637
                     /locus_tag="OO572_000663"
                     /pseudo
     CDS             44222..46637
                     /locus_tag="OO572_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47350..48444)
                     /gene="mnmH"
                     /locus_tag="OO572_000664"
     CDS             complement(47350..48444)
                     /gene="mnmH"
                     /locus_tag="OO572_000664"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6659775.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48513..49439)
                     /gene="allS"
                     /locus_tag="OO572_000665"
     CDS             complement(48513..49439)
                     /gene="allS"
                     /locus_tag="OO572_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6659776.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49669..50151
                     /gene="allA"
                     /locus_tag="OO572_000666"
     CDS             49669..50151
                     /gene="allA"
                     /locus_tag="OO572_000666"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6659777.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50229..51044
                     /gene="allR"
                     /locus_tag="OO572_000667"
     CDS             50229..51044
                     /gene="allR"
                     /locus_tag="OO572_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6659778.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51134..52915
                     /gene="gcl"
                     /locus_tag="OO572_000668"
     CDS             51134..52915
                     /gene="gcl"
                     /locus_tag="OO572_000668"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6659779.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52928..>53190
                     /locus_tag="OO572_000669"
     CDS             52928..>53190
                     /locus_tag="OO572_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6659780.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT        12824 a        13685 c        14007 g        12674 t
ORIGIN      
        1 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
       61 ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg aaattgtgcc
      121 atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac gctttaacac
      181 accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat caccgtaagt
      241 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
      301 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
      361 gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca attagccggg
      421 gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg gctctgttgg
      481 ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag ccaaggcaga
      541 tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct cccaccgttt
      601 cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa gaattgtctg
      661 gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt tgtggttgcc
      721 gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc gccattgttt
      781 aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc ggatggcaaa
      841 gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt ctttagctgg
      901 attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat gtcggaccca
      961 cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat ctatggcgga
     1021 ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc cgtcagccca
     1081 aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa tgccttgctg
     1141 tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc ttgtttactg
     1201 gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc gatgatatgc
     1261 ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt cagaatgtcg
     1321 acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc atcaagataa
     1381 atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt cggatcggca
     1441 aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata gtgattgatc
     1501 atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc aagattgatc
     1561 gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa gacgcttctt
     1621 ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg aataaaacta
     1681 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
     1741 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
     1801 atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg cagcacaaac
     1861 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
     1921 cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt catctccagg
     1981 aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg tgttttttgt
     2041 agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac gttatgggca
     2101 ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc aataaaggaa
     2161 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta acggataatg
     2221 cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat tttacctagc
     2281 tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg aacgttgtta
     2341 tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg catttccatg
     2401 agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag cgcatctttc
     2461 ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt atccagctct
     2521 tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc gacaggcagc
     2581 aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt tctcaccagc
     2641 atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag gctggcagga
     2701 gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca ttgtcatttg
     2761 accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg attcggcgcg
     2821 agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca ttttttgcag
     2881 ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa ccagcgcggc
     2941 aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat ccagcacgtc
     3001 gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat taagcaggat
     3061 aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca acatcaccgc
     3121 cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt tacggataaa
     3181 actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag actcaatcgt
     3241 ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct gcaactttcc
     3301 agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc taccacaatt
     3361 ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct ggattaccgc
     3421 ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct accacgacgt
     3481 tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag acgttattac
     3541 cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg tcgatgggat
     3601 gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg atgcgaatag
     3661 ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg agaaaaatgt
     3721 tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc tgaccgaata
     3781 aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga ttgtatcggt
     3841 gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc gagcgataca
     3901 actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg ttgctgaaaa
     3961 tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac atctgtgtga
     4021 taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta ataaacagcc
     4081 ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact gaggaaggga
     4141 tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg gctgagtaaa
     4201 ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca cgctccagcg
     4261 tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt aaaggttttt
     4321 cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc aggattctct
     4381 ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca tttctgaaga
     4441 gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag atattcgtcg
     4501 atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag tgcgaaggtc
     4561 gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg gtcgttaacc
     4621 caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt ttcacagaga
     4681 aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac gcgtcccctt
     4741 cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga cctagacttg
     4801 ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc gcattattac
     4861 gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact agcactaacc
     4921 cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt atcgccagat
     4981 gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc gaggaatgaa
     5041 taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt attaaatcta
     5101 ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt ttatacgtgg
     5161 ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct taatgttcgt
     5221 aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt atcaatgatg
     5281 atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc ggcgaaccac
     5341 cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca tcccgcccat
     5401 tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga taaccagcgg
     5461 cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac gcatccgcac
     5521 cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt ctttggcgaa
     5581 ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca gtcctacttg
     5641 gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac cgataacccc
     5701 cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca gcgccgccag
     5761 acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt tgccggagag
     5821 acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca gtttgctcgc
     5881 cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg cctggcctaa
     5941 gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt actcccaacg
     6001 agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa cataccagtc
     6061 gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa ctttcttcac
     6121 acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc ccagagtggt
     6181 gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga tatcaatctt
     6241 aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat cagggtgctt
     6301 cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac caaggccagc
     6361 ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga ttgcgggcag
     6421 gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg cacgcatggt
     6481 aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca aggaaacgaa
     6541 cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg cgaacaccga
     6601 ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga gcactttctg
     6661 cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata cgccctggtc
     6721 ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc cgaccacgat
     6781 gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta cgctgtcggt
     6841 gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc cttttttacc
     6901 ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag ccggagtcag
     6961 gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag agaactgacg
     7021 atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg ccgacagtac
     7081 catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg tagcttcttt
     7141 tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa cgatagcgtc
     7201 atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt ttatcgagaa
     7261 gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg caatggtcgg
     7321 aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca cgaggatgat
     7381 cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg gcgtggtgtc
     7441 gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg ccagttcagc
     7501 acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga tccccagacc
     7561 ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac ccagctcaat
     7621 cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca gcaggatttt
     7681 gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt taagctgttg
     7741 gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt tctgcgcttt
     7801 gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat tcgggttcat
     7861 ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc ccgacgtacg
     7921 gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct gcgtcatggt
     7981 gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt tctcaacgct
     8041 caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca gtttgttctg
     8101 cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg tgatctgcac
     8161 ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac cgttcatatt
     8221 ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc cggggtagga
     8281 ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg gcagtttgag
     8341 gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa aaatcgggcg
     8401 atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac ttggactgtt
     8461 caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct tgtgctttta
     8521 cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg cctgctttca
     8581 gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc ggacgggttt
     8641 ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc gtacgggtta
     8701 cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga cgtgcgcgca
     8761 cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt agggtgatag
     8821 acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc tgcgggaact
     8881 taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt ttcagcgtgc
     8941 cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc acatcaacgt
     9001 agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc tgtaccaatg
     9061 cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg actttggtgt
     9121 aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc gcagcattcg
     9181 cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac tgagtaccga
     9241 gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc tgggctttcg
     9301 ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca atctgataga
     9361 gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg ataatcccgc
     9421 taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga agctcggttg
     9481 tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc tgctggccac
     9541 cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag agcatcagaa
     9601 cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga gtgtccgatt
     9661 tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat tatcgtcgtg
     9721 ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc acgaacatat
     9781 ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg atgtggctct
     9841 acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg caaaagcagc
     9901 tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt tgttcagtga
     9961 gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc aggcaaaatt
    10021 ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg aatccacggt
    10081 gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg aatttgtcgg
    10141 agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt atgaccgtat
    10201 agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt taatgacgcg
    10261 tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact ggctctttgc
    10321 cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct tactggagat
    10381 gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct gaatctgact
    10441 ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt cgtgatattc
    10501 ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc atcatgacta
    10561 tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt gcttttgttt
    10621 ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt gatctgccga
    10681 caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat ctttctgctc
    10741 aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat aaaatcgatc
    10801 gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg gaaaaaatgc
    10861 gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa gaaacgcgca
    10921 aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag gcgctggacg
    10981 atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt tcgttacaga
    11041 cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg caacaaattc
    11101 gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc cagaaagtgt
    11161 taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt aaaagcctgg
    11221 aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg acggcgaaca
    11281 gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc aagcgcctga
    11341 ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg cgtattcagg
    11401 ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag cgtctgatta
    11461 ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa aactggctgg
    11521 agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg aagggcagcc
    11581 tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg gatgaactgg
    11641 aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt aaccagcagc
    11701 gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt cacaccaacg
    11761 aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt cgcgaattgc
    11821 tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac ctgcaaatca
    11881 accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact cagcaaatct
    11941 tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc ccgcaaagcc
    12001 tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc tggcctgccg
    12061 tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg atccactggc
    12121 gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt tccctgcgta
    12181 acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc gcgctgccgg
    12241 tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc aacatcagcg
    12301 aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt ggcctgtgct
    12361 ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg gagcagcaga
    12421 ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct atccatttct
    12481 ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg gggcaggcga
    12541 tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc cgcgaaagct
    12601 ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg tcgattatcc
    12661 cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg cgcctctccg
    12721 ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac cagacggtat
    12781 tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg gcgcgtcggc
    12841 agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc accattgcac
    12901 tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt gcgctgttcg
    12961 gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat ctcgacagca
    13021 tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa aacgtcacca
    13081 tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg attcgcaacc
    13141 tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag ggcgcgtcgt
    13201 atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg acggtgttcg
    13261 gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta tccgtaggtc
    13321 ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc attctgttcg
    13381 aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg acggtaagta
    13441 agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg atcatcccga
    13501 acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact actacgcgtc
    13561 tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt aaagtgttac
    13621 tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa gtcttcttta
    13681 cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt gaactgcgtg
    13741 accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc cgtgaaaacg
    13801 acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag ggcgatgagg
    13861 tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt gggtaataac
    13921 caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt gttcatgccg
    13981 gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata tattgcagga
    14041 gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc gcaatcttag
    14101 gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt gcgttaattt
    14161 cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg cagggatctg
    14221 attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag gggcattttc
    14281 aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt tcgatttttt
    14341 ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca cgatgcagcg
    14401 tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg gcaaccatct
    14461 cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag cgggaaattt
    14521 tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc gcgatggctt
    14581 ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt tgctgctcaa
    14641 ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac tgtagtgttg
    14701 tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg aactgtgaca
    14761 ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc agcagcaggg
    14821 cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt agccactatg
    14881 taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct ggctggcggt
    14941 agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt ttatcctgct
    15001 ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc tgtaccgctc
    15061 atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc gcggcgtcaa
    15121 accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt tcgtccagat
    15181 gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct atatgtggcg
    15241 aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg cagttgcaat
    15301 tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg gtcaaaggtt
    15361 aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt aacaagcagg
    15421 catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag catcaaaagc
    15481 attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt actggaagac
    15541 ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa tgcgggcatt
    15601 accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt agctctgggt
    15661 ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga aaccatcagt
    15721 gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga tgccatcaaa
    15781 ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac tatcgaagcg
    15841 accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt cattatcaac
    15901 ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag ctacagcctt
    15961 gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta cggacagcac
    16021 aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc ccttcgtgaa
    16081 tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc cgaaaatggc
    16141 gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg
    16201 gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt ggcgtcggaa
    16261 aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc attaccgcga
    16321 cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga
    16381 ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg ctggataacg
    16441 tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa gtgcatatgc
    16501 tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg gagcacgtta
    16561 agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg tcacgctgtc
    16621 tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt gagcacatcc
    16681 tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg cgcgccgctg
    16741 aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc ggtgacggcc
    16801 aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat caggcgctgt
    16861 cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg attaatgaag
    16921 ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc ttgttgcatc
    16981 gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc gccatcgagc
    17041 tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt tactatcaga
    17101 cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg ggcgttgaga
    17161 tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag ccagaagtgc
    17221 cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc ctggtgccgc
    17281 cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc agccaggtgc
    17341 tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa aagagtgaac
    17401 cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga ctggcttcgg
    17461 tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg ccagccaaaa
    17521 aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa gtggtcgcca
    17581 cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg gcggtgaagc
    17641 tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa ctttcgctac
    17701 caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac aacgcagtat
    17761 gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag caaaaactgg
    17821 ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt gaagatgata
    17881 atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa aaacttgcgc
    17941 aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg tttttcgatg
    18001 cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt acgttcgtca
    18061 tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg taacctgatg
    18121 aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat cgcgcagctg
    18181 gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg tgcacacaac
    18241 tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat gctggaagac
    18301 ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca gaaagaaaaa
    18361 atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc gttctgatgc
    18421 aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg ggcgttggcc
    18481 cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc ggcgggatgc
    18541 gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc gattgccgca
    18601 ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag gaaaacggtc
    18661 aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg gggcagtttt
    18721 ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc ggtccggatg
    18781 atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact gaagtgatcc
    18841 tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc gagctttgcg
    18901 cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc ggcgaactgg
    18961 aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag attcgttttt
    19021 aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt gtccccatct
    19081 ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta catgaaagga
    19141 caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat gatccattct
    19201 ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc cgatgcggcg
    19261 gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga tggcgaactg
    19321 cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga taacggcgtg
    19381 gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc cggtaccaaa
    19441 tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat cggtcagttt
    19501 ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg tactcgcgcg
    19561 gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg tgaatatacc
    19621 gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct gcgtgaaggc
    19681 gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata ctccgaccat
    19741 atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac cgttatctcc
    19801 tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga aatcaccgat
    19861 gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc gctgacctgg
    19921 agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat cccgtcccag
    19981 gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta cgttcaacgc
    20041 gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt cgtgcgtggt
    20101 ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca ggacagcacg
    20161 gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct ggaaaaactg
    20221 gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct ggtactgaaa
    20281 gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct gcgttttgct
    20341 tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt ttcccgcatg
    20401 aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc agcgaagagc
    20461 agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct ttccgaccgc
    20521 atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt ccagtcggtg
    20581 tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag cgcgaaagaa
    20641 gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg cgagcgcgtg
    20701 aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc gaccgacgcg
    20761 gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa agtgccagaa
    20821 gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc ggcagatact
    20881 gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc gctgctggca
    20941 gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca gctgctggtt
    21001 tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc atcatctgta
    21061 ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc ttgaggcaat
    21121 cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt taaggggatt
    21181 ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac tcaggctcag
    21241 ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct gcgtgctgcg
    21301 gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc tggcaaactg
    21361 gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga agactgccgt
    21421 aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc gatgaaagaa
    21481 gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact gattgttgat
    21541 cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt taaattcaat
    21601 ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac ccgtaaagac
    21661 gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac tgcaccgctg
    21721 atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa agttgacggt
    21781 accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta atcaagccgc
    21841 agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc tcacagcaat
    21901 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
    21961 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
    22021 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
    22081 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
    22141 gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg atggtttaca
    22201 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg gcgctgggca
    22261 tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca gagcatgtag
    22321 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
    22381 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
    22441 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
    22501 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
    22561 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
    22621 ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
    22681 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
    22741 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
    22801 ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat gaatatattc
    22861 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
    22921 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
    22981 tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
    23041 gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc agcggatcga
    23101 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
    23161 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
    23221 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
    23281 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
    23341 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
    23401 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
    23461 cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg gtgtaattaa
    23521 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
    23581 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
    23641 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
    23701 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
    23761 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
    23821 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
    23881 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
    23941 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    24001 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    24061 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    24121 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    24181 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    24241 atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    24301 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
    24361 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    24421 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggtccgattg
    24481 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    24541 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    24601 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    24661 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    24721 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    24781 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    24841 catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg ccaatcggct
    24901 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    24961 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    25021 gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    25081 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    25141 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    25201 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    25261 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc caggaagagt
    25321 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    25381 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    25441 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc cgccggtggc
    25501 gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac tacctgattc
    25561 gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc gataatctca
    25621 atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata accgttgggg
    25681 atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat ttcttcgttc
    25741 gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac acgggttcgt
    25801 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    25861 cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg gatctgtttc
    25921 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    25981 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    26041 accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    26101 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    26161 tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag aataattgcc
    26221 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    26281 actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc gtgggcggca
    26341 cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    26401 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcactgac
    26461 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    26521 cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat ggtgatcccc
    26581 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    26641 ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca accgatggcg
    26701 atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    26761 gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga ccagcccagc
    26821 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    26881 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    26941 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    27001 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    27061 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    27121 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    27181 ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttta
    27241 tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggttcgga
    27301 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    27361 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    27421 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat gctggtgtga
    27481 tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc aaaaccgaaa
    27541 aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta gaccagaatg
    27601 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    27661 aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    27721 acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    27781 gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc gaatttttgc
    27841 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    27901 aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg attgattatc
    27961 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctaatttgc
    28021 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    28081 taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    28141 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    28201 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc cagcagaact
    28261 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    28321 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    28381 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    28441 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    28501 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    28561 gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa gtcacttttt
    28621 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    28681 tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    28741 atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc aacacgttga
    28801 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    28861 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    28921 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    28981 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    29041 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    29101 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    29161 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    29221 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    29281 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct gacaaccgat
    29341 gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg taagcccgcc
    29401 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    29461 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    29521 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    29581 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    29641 ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    29701 tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt taactaccag
    29761 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    29821 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    29881 aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg tctggaaggc
    29941 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    30001 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    30061 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    30121 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    30181 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    30241 aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    30301 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    30361 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    30421 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    30481 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    30541 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    30601 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    30661 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    30721 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    30781 cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    30841 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    30901 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    30961 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    31021 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    31081 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    31141 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    31201 aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    31261 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    31321 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt ttgcggcggt
    31381 gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg ccagtgggtc
    31441 agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    31501 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    31561 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    31621 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    31681 tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    31741 caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    31801 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    31861 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt gatatcgatg
    31921 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    31981 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg gatggcgcaa
    32041 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg catcgctatt
    32101 cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga cgacacccgg
    32161 agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg agttagcaga
    32221 gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc gtaaactcac
    32281 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt ttaggctaaa
    32341 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    32401 gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg caaaaaaggt
    32461 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca cgccgcatcc
    32521 ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc tacaaacctg
    32581 tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat gcgtcgatgc
    32641 caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt cggtttaaac
    32701 cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat tgccgctccg
    32761 gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc ggcgaccgga
    32821 ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt gcgcagcgtt
    32881 gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat cagggtaatc
    32941 gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac atccgcctgg
    33001 gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg tccttcactt
    33061 tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat cacttcatca
    33121 attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt caacatcacc
    33181 agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact acgcaacgga
    33241 tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac aggcgttgct
    33301 ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac ttgctgatca
    33361 tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc gctcacgccc
    33421 agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc tgctttatca
    33481 aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc cagacgcagc
    33541 gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg cttcccttcg
    33601 gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg ttgcagcgcg
    33661 tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga aataatcgac
    33721 atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt agtggcaatc
    33781 accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc cgcactgaca
    33841 agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc cagctgaccg
    33901 atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga tagcgtggta
    33961 tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac tgtcccggca
    34021 tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag catcgcttca
    34081 tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc gccggtcgtc
    34141 aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc accttcgtca
    34201 gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc cttagaagaa
    34261 cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat aatcatcgca
    34321 ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca caggttgacg
    34381 ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc catcgtcgct
    34441 gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc gaaaaccatc
    34501 accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc ggtgactatc
    34561 atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag tgcgacaatt
    34621 gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg ttcgcggcgt
    34681 ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc ctgcactaaa
    34741 tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt tacccgtgcc
    34801 tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca gctggcgcag
    34861 ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc tgccggaagc
    34921 gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag cggtttagcc
    34981 tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat cagctgttct
    35041 gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg ttccacatcc
    35101 gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga caggccgtcc
    35161 aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt gactggctgt
    35221 gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc aggcgggtaa
    35281 aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat ttatttcttt
    35341 gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa aatcggcact
    35401 ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg ttagatgcaa
    35461 acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt aacggcggac
    35521 aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg gcagtggcta
    35581 tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc tttgcactgg
    35641 aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc ccgcaggcgg
    35701 tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt atcgccttag
    35761 agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt gccaccacca
    35821 gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat atccaacagc
    35881 aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa caaacaacca
    35941 acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc tattgtgatg
    36001 caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat actgttgaac
    36061 tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg cataaacgcg
    36121 ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa gggctgtatg
    36181 gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt gtaggtggcg
    36241 gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca ccgctggacg
    36301 aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag caactcggct
    36361 ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta acaaacccct
    36421 cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg ggatcttcgc
    36481 gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct ttgcttttgg
    36541 cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga gctatcccag
    36601 caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct tttcgctagc
    36661 gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg cccgtgcttt
    36721 tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc ttattttgct
    36781 ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca accatgcggt
    36841 agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat tgctgattat
    36901 tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc ccagctccgg
    36961 tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg gcatgatggc
    37021 gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt ttctggtggc
    37081 gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta gcgatgtatc
    37141 ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt ctccgctgct
    37201 cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg cttcaggcat
    37261 taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg aacgcgaact
    37321 gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata tcatcgtcgt
    37381 ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca gcgttgccag
    37441 cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc tgcgtcatga
    37501 tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt tggtgatcgt
    37561 tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga ttattggtgc
    37621 aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg tataacacct
    37681 ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca ctggcaaaaa
    37741 aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt gcgcgaacga
    37801 tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt cgcttctatg
    37861 aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc acctacacgc
    37921 agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc tttaacctgg
    37981 aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc gccgacgtca
    38041 aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag ctgcaatcca
    38101 tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc gccgactgcc
    38161 cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta agacgagacg
    38221 gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt acctgcaccg
    38281 agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg
    38341 ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt
    38401 aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc
    38461 caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca acccagcggc
    38521 accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa caaataacca
    38581 tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca gaaaatatgt
    38641 ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca
    38701 gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta
    38761 ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt tgcgctacga
    38821 agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt ttggtggcgc
    38881 gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt
    38941 cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct
    39001 gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg
    39061 cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga
    39121 ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt gagttgatgc
    39181 tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag
    39241 tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca gcgcgtggcg
    39301 cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga
    39361 taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc
    39421 gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa
    39481 aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga
    39541 ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta
    39601 ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga
    39661 gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa
    39721 cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg
    39781 atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta
    39841 ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc
    39901 caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa ccagaaatct
    39961 accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat acggtatacg
    40021 ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc atttttctcg
    40081 attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc gccgagctat
    40141 ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt
    40201 tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac gtcaatgaga
    40261 tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag
    40321 acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg
    40381 aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc attaatgctg
    40441 gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattcta
    40501 tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct gaagtatatt
    40561 ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg
    40621 gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt
    40681 gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg ggcgattgaa
    40741 tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc catggtagcg
    40801 gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag
    40861 gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat tcgcgacagt
    40921 attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta
    40981 ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat
    41041 cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc
    41101 tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac tcaattgaag
    41161 aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga
    41221 acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca
    41281 tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg accgtctgcg
    41341 gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca
    41401 acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg attctccgcc
    41461 acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag cagttcgatt
    41521 tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc
    41581 gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc
    41641 tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa
    41701 ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc
    41761 actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc
    41821 ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca
    41881 atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc
    41941 gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa
    42001 tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta
    42061 atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac
    42121 atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgccca
    42181 ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg atagcgcatc
    42241 ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc cggtcccaac
    42301 gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg
    42361 ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg
    42421 tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg
    42481 taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat cacaatacgc
    42541 ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa
    42601 aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac
    42661 atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc
    42721 tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac gccggtaaat
    42781 cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag cacatgaaaa
    42841 ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt
    42901 aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt
    42961 tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt
    43021 gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt ccataaaaaa
    43081 gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc
    43141 attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt
    43201 ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa
    43261 accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg atgctgtttg
    43321 agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat gctggcatta
    43381 actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc cgcgctggca
    43441 gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc cgctgcggcg
    43501 gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa aacattgttg
    43561 aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc atcctcaccg
    43621 gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag tcgggatcgg
    43681 gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt ggcgaagtga
    43741 gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag ttgcgcgcga
    43801 agcacgtcgg ctttgttttt cagttattta tgttaattcc tacccttaac gcgctggaaa
    43861 acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt aacggggcga
    43921 aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg gcacagcttt
    43981 ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga cctgatgtgc
    44041 tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa attgccgacc
    44101 tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc cacgacctgc
    44161 aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg caggaggaag
    44221 catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa ttgtctggct
    44281 ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg atcgcatgga
    44341 gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt tgcgcagttc
    44401 acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga aagtcggcaa
    44461 gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg cgaacgtcaa
    44521 agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc ctggcctgaa
    44581 accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta acctgaaaac
    44641 gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag tgattcagga
    44701 accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga atctggcgga
    44761 tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt ataaattcgg
    44821 cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc ttaaacccga
    44881 acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga tggaacgctc
    44941 gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg cagcggtggc
    45001 ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc tcaaaacgct
    45061 gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga tggtgctgac
    45121 gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt tgatggtgct
    45181 gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt ggctgtgggc
    45241 gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc gcttgttgct
    45301 ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct ggccgctgaa
    45361 gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat taatgggtgg
    45421 cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc tgtgcggtgt
    45481 gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc tgcctctgcg
    45541 cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc tttcggcatt
    45601 ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc tgctcgaccg
    45661 ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca tcgccacaga
    45721 acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg aatcatttta
    45781 tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag gtaatgaaga
    45841 tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg atcataatcc
    45901 gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag aggggctggc
    45961 gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata cccaggagtt
    46021 ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc ctaatttcta
    46081 ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta ccagtttccg
    46141 ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga ccattagcct
    46201 gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg taagtcgggc
    46261 gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc tggcacaggt
    46321 gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac tcggtgcggg
    46381 gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg tttgtttccg
    46441 gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg aaagtgtttg
    46501 atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc ggtgcgctgc
    46561 tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag gcgctgttca
    46621 ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct tatcaggcct
    46681 acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa ggcgtttacg
    46741 ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac tgtcatgtcg
    46801 gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc tcagttattc
    46861 aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga tgagaacttt
    46921 atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac agcttaccct
    46981 tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct ctctccaaat
    47041 tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta cttttccgca
    47101 ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg ccaacatagc
    47161 tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga ctgtttaatt
    47221 tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg tcatgtcggt
    47281 ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc cgtttcagaa
    47341 taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac cgcgaaagac
    47401 aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat attctttaag
    47461 taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt gcgttgtcag
    47521 cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc gacgcttaat
    47581 cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt cgtcgccata
    47641 agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc gttcaagacg
    47701 gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct cgcgcagaca
    47761 ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc acaggcgcaa
    47821 atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa aactcgcctg
    47881 gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg ccagcccttc
    47941 cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc cggtacaacc
    48001 gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg cggtctggcg
    48061 cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat gcagccagcg
    48121 ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt gcggattttg
    48181 caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg ccaccagttt
    48241 atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc caacggaggc
    48301 gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc cttgctcaaa
    48361 ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg cacgatagtc
    48421 ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct tgcgcgctat
    48481 tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc gaaaatttct
    48541 aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc ttctaccgct
    48601 ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg catcgttggg
    48661 attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga ttttggcaaa
    48721 aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc gggaacaata
    48781 atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc ggcgctgtct
    48841 tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc ttcaacgttc
    48901 gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa gggatcaaga
    48961 ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga aaaaccttcg
    49021 tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa ctgggtaaag
    49081 gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt atagagcagg
    49141 ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg cagctcgctg
    49201 ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg ctcgccagcc
    49261 gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt attctcttcc
    49321 agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc tgccgctttt
    49381 gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg atcgaacatc
    49441 ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct gatttatcag
    49501 tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg aaaaacattc
    49561 caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt gaaaccaagc
    49621 tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat gaaacttcag
    49681 gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga aacgcagcaa
    49741 cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt ggcgctggtt
    49801 gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc ggcgaatctg
    49861 ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt tatcccaatg
    49921 aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga cctgtcaacg
    49981 ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa cgtctggcat
    50041 cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg cggcggcagt
    50101 gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg acgcctgcaa
    50161 ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag gtcggagtta
    50221 acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag cctgtggcac
    50281 agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg gaaaaaagtg
    50341 ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc tccacgacct
    50401 ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt caattaggct
    50461 ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat aaccgcgatg
    50521 tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc gaaacggtca
    50581 atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag tgtaaatcga
    50641 tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc ggtgcgggca
    50701 aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg caaaccggtt
    50761 tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag gacctggaac
    50821 aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt ctgaattgca
    50881 tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc atctccgggc
    50941 cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc agagacaccg
    51001 cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg tcgcatcccg
    51061 ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc caactaatag
    51121 aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg tgctggagaa
    51181 agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt tctactcagc
    51241 gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag gtgtttcgca
    51301 catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc tggggacttc
    51361 cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg attccattcc
    51421 tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag attttcaggc
    51481 cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag ttcgtgaagc
    51541 ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg gtcgtccggg
    51601 tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt ttgatcctga
    51661 catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga tcgaaaaagc
    51721 tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg gggtaatcaa
    51781 tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc cggtgatccc
    51841 aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg ggatggtcgg
    51901 tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca tggtgtttgg
    51961 tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca ccgaagggcg
    52021 caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt gtccggatct
    52081 cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg cgcaggagat
    52141 gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc agcagcgtaa
    52201 acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc agcgcgtgta
    52261 tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta ttggtctgtc
    52321 acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga tcaactgtgg
    52381 tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg ccgctgatcc
    52441 gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga ttgaagagtt
    52501 agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata acgcctatct
    52561 cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc aactcgcttt
    52621 cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa aagtagcgga
    52681 aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc cagcctttga
    52741 acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag ttattctcga
    52801 gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat ttgaagatat
    52861 cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg aataagggag
    52921 ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat atgattttct
    52981 cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta tgtttcctta
    53041 tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg aacatacgct
    53101 gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct gtattcctgg
    53161 ccgtgaagaa gagtttcggg atggcgtagc
//
LOCUS       DAJTYL010000011        52589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYL010000011 DAJTYL010000000
VERSION     DAJTYL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.011"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..346
                     /locus_tag="OO572_000670"
     CDS             <1..346
                     /locus_tag="OO572_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6659781.1"
                     /translation="HHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELE
                     NSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQ
                     IMSRVKIVVMYN"
     gene            complement(384..1283)
                     /gene="nhaR"
                     /locus_tag="OO572_000671"
     CDS             complement(384..1283)
                     /gene="nhaR"
                     /locus_tag="OO572_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6659782.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1349..2515)
                     /gene="nhaA"
                     /locus_tag="OO572_000672"
     CDS             complement(1349..2515)
                     /gene="nhaA"
                     /locus_tag="OO572_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6659783.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3101..3253
                     /gene="mokC"
                     /locus_tag="OO572_000673"
     CDS             3101..3253
                     /gene="mokC"
                     /locus_tag="OO572_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6659784.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3357..4487)
                     /gene="dnaJ"
                     /locus_tag="OO572_000674"
     CDS             complement(3357..4487)
                     /gene="dnaJ"
                     /locus_tag="OO572_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6659785.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4576..6492)
                     /gene="dnaK"
                     /locus_tag="OO572_000675"
     CDS             complement(4576..6492)
                     /gene="dnaK"
                     /locus_tag="OO572_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6659786.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6869..7273
                     /locus_tag="OO572_000676"
     CDS             6869..7273
                     /locus_tag="OO572_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6659787.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7299..8012
                     /gene="msyB"
                     /locus_tag="OO572_000677"
     CDS             7299..8012
                     /gene="msyB"
                     /locus_tag="OO572_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6659788.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8161..8727
                     /gene="satP"
                     /locus_tag="OO572_000678"
     CDS             8161..8727
                     /gene="satP"
                     /locus_tag="OO572_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6659789.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8762..9349)
                     /gene="mog"
                     /locus_tag="OO572_000679"
     CDS             complement(8762..9349)
                     /gene="mog"
                     /locus_tag="OO572_000679"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6659790.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9464..10417)
                     /gene="tal"
                     /locus_tag="OO572_000680"
     CDS             complement(9464..10417)
                     /gene="tal"
                     /locus_tag="OO572_000680"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6659791.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10696..12126
                     /locus_tag="OO572_000681"
                     /pseudo
     CDS             10696..12126
                     /locus_tag="OO572_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12196..12972
                     /gene="yaaA"
                     /locus_tag="OO572_000682"
     CDS             12196..12972
                     /gene="yaaA"
                     /locus_tag="OO572_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6659792.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13125..13421)
                     /locus_tag="OO572_000683"
     CDS             complement(13125..13421)
                     /locus_tag="OO572_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6659793.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13635..14921)
                     /gene="thrC"
                     /locus_tag="OO572_000684"
     CDS             complement(13635..14921)
                     /gene="thrC"
                     /locus_tag="OO572_000684"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6659794.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14922..15854)
                     /gene="thrB"
                     /locus_tag="OO572_000685"
     CDS             complement(14922..15854)
                     /gene="thrB"
                     /locus_tag="OO572_000685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6659795.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15856..18318)
                     /gene="thrA"
                     /locus_tag="OO572_000686"
     CDS             complement(15856..18318)
                     /gene="thrA"
                     /locus_tag="OO572_000686"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6659796.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18344..18463)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18399..18464)
                     /gene="thrL"
                     /locus_tag="OO572_000687"
     CDS             complement(18399..18464)
                     /gene="thrL"
                     /locus_tag="OO572_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6659797.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18678..19364)
                     /locus_tag="OO572_000688"
     CDS             complement(18678..19364)
                     /locus_tag="OO572_000688"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6659798.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19764..19904)
                     /locus_tag="OO572_000689"
     CDS             complement(19764..19904)
                     /locus_tag="OO572_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659799.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20000..20716
                     /gene="arcA"
                     /locus_tag="OO572_000690"
     CDS             20000..20716
                     /gene="arcA"
                     /locus_tag="OO572_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6659800.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20775..22127)
                     /gene="creD"
                     /locus_tag="OO572_000691"
     CDS             complement(20775..22127)
                     /gene="creD"
                     /locus_tag="OO572_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6659801.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22185..23609)
                     /gene="creC"
                     /locus_tag="OO572_000692"
     CDS             complement(22185..23609)
                     /gene="creC"
                     /locus_tag="OO572_000692"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6659802.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23609..24298)
                     /gene="creB"
                     /locus_tag="OO572_000693"
     CDS             complement(23609..24298)
                     /gene="creB"
                     /locus_tag="OO572_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6659803.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24311..24784)
                     /gene="creA"
                     /locus_tag="OO572_000694"
     CDS             complement(24311..24784)
                     /gene="creA"
                     /locus_tag="OO572_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6659804.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24995..25864
                     /gene="robA"
                     /locus_tag="OO572_000695"
     CDS             24995..25864
                     /gene="robA"
                     /locus_tag="OO572_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6659805.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25861..26508)
                     /gene="gpmB"
                     /locus_tag="OO572_000696"
     CDS             complement(25861..26508)
                     /gene="gpmB"
                     /locus_tag="OO572_000696"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6659806.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26560..27080
                     /gene="yjjX"
                     /locus_tag="OO572_000697"
                     /pseudo
     CDS             26560..27080
                     /gene="yjjX"
                     /locus_tag="OO572_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27165..27491)
                     /gene="trpR"
                     /locus_tag="OO572_000698"
     CDS             complement(27165..27491)
                     /gene="trpR"
                     /locus_tag="OO572_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6659807.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27581..29518)
                     /gene="sltY"
                     /locus_tag="OO572_000699"
     CDS             complement(27581..29518)
                     /gene="sltY"
                     /locus_tag="OO572_000699"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6659808.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29729..31396
                     /gene="ettA"
                     /locus_tag="OO572_000700"
     CDS             29729..31396
                     /gene="ettA"
                     /locus_tag="OO572_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6659809.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31503..31670)
                     /locus_tag="OO572_000701"
     CDS             complement(31503..31670)
                     /locus_tag="OO572_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659810.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31704..32936)
                     /gene="nadR"
                     /locus_tag="OO572_000702"
     CDS             complement(31704..32936)
                     /gene="nadR"
                     /locus_tag="OO572_000702"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6659811.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32957..34339)
                     /gene="radA"
                     /locus_tag="OO572_000703"
     CDS             complement(32957..34339)
                     /gene="radA"
                     /locus_tag="OO572_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6659812.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34388..35356)
                     /gene="serB"
                     /locus_tag="OO572_000704"
     CDS             complement(34388..35356)
                     /gene="serB"
                     /locus_tag="OO572_000704"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6659813.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35462..36106
                     /locus_tag="OO572_000705"
     CDS             35462..36106
                     /locus_tag="OO572_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6659814.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36134..37149
                     /gene="lplA"
                     /locus_tag="OO572_000706"
                     /pseudo
     CDS             36134..37149
                     /gene="lplA"
                     /locus_tag="OO572_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37181..37444
                     /locus_tag="OO572_000707"
     CDS             37181..37444
                     /locus_tag="OO572_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6659815.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37605..38324)
                     /gene="deoD"
                     /locus_tag="OO572_000708"
     CDS             complement(37605..38324)
                     /gene="deoD"
                     /locus_tag="OO572_000708"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6659816.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38381..39604)
                     /gene="deoB"
                     /locus_tag="OO572_000709"
     CDS             complement(38381..39604)
                     /gene="deoB"
                     /locus_tag="OO572_000709"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6659817.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39656..40978)
                     /gene="deoA"
                     /locus_tag="OO572_000710"
     CDS             complement(39656..40978)
                     /gene="deoA"
                     /locus_tag="OO572_000710"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6659818.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41105..41884)
                     /gene="deoC"
                     /locus_tag="OO572_000711"
     CDS             complement(41105..41884)
                     /gene="deoC"
                     /locus_tag="OO572_000711"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6659819.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42142..43683
                     /locus_tag="OO572_000712"
     CDS             42142..43683
                     /locus_tag="OO572_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6659820.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43655..44518
                     /locus_tag="OO572_000713"
     CDS             43655..44518
                     /locus_tag="OO572_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6659821.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44732..45514)
                     /locus_tag="OO572_000714"
     CDS             complement(44732..45514)
                     /locus_tag="OO572_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6659822.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45511..46584)
                     /locus_tag="OO572_000715"
     CDS             complement(45511..46584)
                     /locus_tag="OO572_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6659823.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46706..46867)
                     /locus_tag="OO572_000716"
     CDS             complement(46706..46867)
                     /locus_tag="OO572_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6659824.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46984..47589)
                     /gene="osmY"
                     /locus_tag="OO572_000717"
     CDS             complement(46984..47589)
                     /gene="osmY"
                     /locus_tag="OO572_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6659825.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47982..49571)
                     /gene="prfC"
                     /locus_tag="OO572_000718"
     CDS             complement(47982..49571)
                     /gene="prfC"
                     /locus_tag="OO572_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6659826.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49662..50339)
                     /gene="yjjG"
                     /locus_tag="OO572_000719"
     CDS             complement(49662..50339)
                     /gene="yjjG"
                     /locus_tag="OO572_000719"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6659827.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50354..50800)
                     /gene="rimI"
                     /locus_tag="OO572_000720"
     CDS             complement(50354..50800)
                     /gene="rimI"
                     /locus_tag="OO572_000720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6659828.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50769..51182)
                     /gene="holD"
                     /locus_tag="OO572_000721"
     CDS             complement(50769..51182)
                     /gene="holD"
                     /locus_tag="OO572_000721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6659829.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51285..52316
                     /gene="rsmC"
                     /locus_tag="OO572_000722"
     CDS             51285..52316
                     /gene="rsmC"
                     /locus_tag="OO572_000722"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6659830.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12525 a        14233 c        13179 g        12652 t
ORIGIN      
        1 tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt ttgaacccag
       61 tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca gcatattaaa
      121 aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac tctccaatga
      181 tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc atccccatga
      241 aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca atgtctcaag
      301 cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta tctcccccac
      361 cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc agaataatcc
      421 gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa aatagcatgg
      481 tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc ggcataaaag
      541 tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc aaaagctttc
      601 atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg ggagttaaac
      661 cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag tcgccgttct
      721 tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa actcacgcca
      781 cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat cggacagtca
      841 gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc gtgggtggat
      901 tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag tacgctactg
      961 accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt ggattctttg
     1021 cgatagttca caatatccag catttcctgg cttaaggtga acattttatc ggcatagcga
     1081 tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt aaataatttg
     1141 ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg agttaaataa
     1201 agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa gtaatacaag
     1261 tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga aaagggagcc
     1321 gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga acgcgtaacc
     1381 agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt ttcgcccagt
     1441 taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata gacatagtaa
     1501 aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt ccctcaggca
     1561 gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc agcggtttgc
     1621 caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca tccagcgtga
     1681 cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc agatacgcca
     1741 cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc ttctctttca
     1801 aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc gatttcaaca
     1861 ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt acaccacaca
     1921 gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag gccatcgata
     1981 agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg ataatagcca
     2041 gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc gccagtacac
     2101 caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg gtaatcggat
     2161 cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca atagcggcga
     2221 taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt tcacgtttaa
     2281 cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc cataacagca
     2341 tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc agaaagtcgt
     2401 gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata gcggcaatga
     2461 taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt ttcacttttt
     2521 atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa tcatcgttaa
     2581 aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa tttgcgccat
     2641 cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta agcccagata
     2701 atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata attaatttgt
     2761 tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt gtcgtcaata
     2821 attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat tacgccacaa
     2881 aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa gagagcgata
     2941 taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga
     3001 aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga acacatgtag
     3061 agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggcgat
     3121 gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa
     3181 agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta
     3241 cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata
     3301 tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag
     3361 cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg
     3421 ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc
     3481 ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg
     3541 ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt
     3601 tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcgccaccc
     3661 agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg
     3721 aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccatgc
     3781 tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg
     3841 attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac
     3901 ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg tacagcgaag
     3961 aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt
     4021 gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg
     4081 atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa
     4141 tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg
     4201 tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc catgcctcct
     4261 tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga
     4321 acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc accctggtta
     4381 cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc acgctcttcc
     4441 gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg
     4501 cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataattac
     4561 ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt cgacaacatc
     4621 gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg
     4681 ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca ttttcgcttc
     4741 gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat
     4801 agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct
     4861 gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc
     4921 ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga
     4981 agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg
     5041 caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc cgcgcggtgc
     5101 cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg cacgtttacg
     5161 ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg tagagaacac
     5221 ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca tcacaccgcc
     5281 catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt cttttacgtc
     5341 accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac
     5401 gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg
     5461 agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc cagcatcctg
     5521 cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca ggctttccag
     5581 tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta
     5641 tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc
     5701 ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt
     5761 gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt
     5821 atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcgat
     5881 aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac ggttgccagt
     5941 gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga tacgttttac
     6001 ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta
     6061 tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt
     6121 cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac
     6181 ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc
     6241 acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt
     6301 gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc
     6361 ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct ccagcacgcg
     6421 aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt cgataccaat
     6481 tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt
     6541 aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag actcactcac
     6601 tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat gcaaaaattt
     6661 tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa ttgacagcaa
     6721 ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt gtaataaaaa
     6781 gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg tattcatccc
     6841 cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt ccgccagcct
     6901 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac tcctcggcgt
     6961 cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat
     7021 tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg gtgcatcggt
     7081 ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga
     7141 agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc
     7201 aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat
     7261 cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg
     7321 attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat ttcgcccgat
     7381 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg cgcaacgaac
     7441 tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag ctgattgccg
     7501 gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     7561 tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga
     7621 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     7681 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     7741 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt
     7801 tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc
     7861 atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta aatggggtta
     7921 aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc
     7981 tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt
     8041 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     8101 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     8161 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     8221 accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat tattcttgcc
     8281 atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa
     8341 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     8401 gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt
     8461 gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac tctgaaaggc
     8521 gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     8581 ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg tctgatctgc
     8641 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     8701 gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg gggggatttt
     8761 tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc acttctggtg
     8821 ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac
     8881 catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact
     8941 tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc acctgacgcg
     9001 aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag
     9061 gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc
     9121 cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg
     9181 tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg
     9241 ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga taaacgccgc
     9301 tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc
     9361 cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc
     9421 agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga
     9481 tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc gccagtttat
     9541 ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg
     9601 gacgcgcttt cacttcgcca gtgtaagaca gtttacgttc gatagcccct tcgctctccg
     9661 ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc agttccagaa
     9721 tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt
     9781 agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt
     9841 tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga
     9901 acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac
     9961 agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca
    10021 gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt
    10081 ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact tcagttgaga
    10141 tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg
    10201 cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat
    10261 caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg
    10321 tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg
    10381 tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc
    10441 ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc
    10501 gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat
    10561 cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt
    10621 agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca
    10681 ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg
    10741 gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg
    10801 cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca
    10861 ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc
    10921 aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt cttttggatg
    10981 tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt
    11041 tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc
    11101 gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc
    11161 ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat
    11221 tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc aatcactcgt
    11281 ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg
    11341 gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg
    11401 tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc
    11461 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggtgggaatg
    11521 ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc ggcagcacaa
    11581 gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
    11641 atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt
    11701 atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt taccctcgtg
    11761 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
    11821 ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca
    11881 acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc agatatcata
    11941 atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat
    12001 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
    12061 ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag
    12121 gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc
    12181 ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc
    12241 ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc
    12301 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa
    12361 ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggcgaat
    12421 gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc
    12481 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
    12541 ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
    12601 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
    12661 aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat
    12721 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat
    12781 gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg
    12841 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
    12901 gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt taaacgctac
    12961 gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca
    13021 aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac gcgccagcgt
    13081 cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg
    13141 cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc
    13201 ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
    13261 tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc gcctatctgt
    13321 aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga
    13381 accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat
    13441 tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg ttattctccg
    13501 caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc ctgtcgtttt
    13561 tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
    13621 ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga aaaatcggcg
    13681 ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc
    13741 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
    13801 aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata cgctacggca
    13861 gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
    13921 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
    13981 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
    14041 tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
    14101 ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag cagacccgcc
    14161 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcctcctgc
    14221 ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta
    14281 atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
    14341 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg
    14401 cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
    14461 accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc ggtatcaccg
    14521 gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag catttgtgcc
    14581 ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa
    14641 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc
    14701 tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
    14761 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
    14821 tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
    14881 ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt
    14941 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
    15001 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag agcgaacaag
    15061 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
    15121 gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac atctttcatc
    15181 agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt
    15241 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct
    15301 gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc aaaccctggc
    15361 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
    15421 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc gcccatcaaa
    15481 gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc
    15541 gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt cttttccaga
    15601 gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca gcactgataa
    15661 acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag tttgttgaga
    15721 ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
    15781 gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
    15841 taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa
    15901 gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg
    15961 ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg
    16021 atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat caatattgcc
    16081 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
    16141 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
    16201 gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg tttcacgagc
    16261 gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc
    16321 ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa
    16381 cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca ccaattcatc
    16441 acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
    16501 gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat gatagtaatc
    16561 catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa
    16621 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
    16681 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
    16741 tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga gcagagcctt
    16801 cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg
    16861 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
    16921 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
    16981 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
    17041 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
    17101 cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca gcggctccag
    17161 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca
    17221 gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
    17281 cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc caaccatccc
    17341 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
    17401 gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg cttgaggatt
    17461 tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg
    17521 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt
    17581 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt
    17641 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga
    17701 accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga aacctgccat
    17761 caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg actcagcaat
    17821 atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac
    17881 ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg acattttctc
    17941 gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca aactaatgcc
    18001 atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca attgcgccag
    18061 cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc
    18121 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
    18181 gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
    18241 gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg atgtaccgcc
    18301 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac
    18361 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
    18421 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    18481 gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    18541 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta tcagacactc
    18601 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    18661 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    18721 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt
    18781 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct
    18841 tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
    18901 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
    18961 atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag cccggaatct
    19021 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    19081 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc agtggtggcg
    19141 acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt aatattatca
    19201 ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
    19261 tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc tgccccaata
    19321 ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc actcttcttg
    19381 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
    19441 ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttt
    19501 ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt ttacttagga
    19561 taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg
    19621 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
    19681 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
    19741 ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt
    19801 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
    19861 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
    19921 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
    19981 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
    20041 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
    20101 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
    20161 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
    20221 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
    20281 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
    20341 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
    20401 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc
    20461 tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga
    20521 aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg gccgtgagct
    20581 gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
    20641 tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg
    20701 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt
    20761 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
    20821 gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac aaacatcatg
    20881 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg
    20941 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
    21001 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
    21061 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
    21121 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
    21181 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
    21241 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
    21301 gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
    21361 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg tttggctggt
    21421 agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    21481 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc catattcagc
    21541 tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    21601 tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc
    21661 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg
    21721 atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    21781 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    21841 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    21901 gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    21961 acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    22021 tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact
    22081 gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt
    22141 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    22201 tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    22261 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    22321 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    22381 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    22441 gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    22501 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac atgcaaagtg
    22561 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc
    22621 acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    22681 gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt aaaacgagcc
    22741 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    22801 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    22861 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    22921 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    22981 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat ccccaacaaa
    23041 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    23101 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat cggcgcggca
    23161 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac
    23221 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc aacggcttta
    23281 tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg atattcattg
    23341 cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag ctgattaaac
    23401 gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    23461 gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta
    23521 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    23581 cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat cccatgccgc
    23641 gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcagcgttt
    23701 taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    23761 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    23821 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    23881 tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc ttcacccgac
    23941 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    24001 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    24061 acagtacagg taacgccgga tagagcgcca gtaactggcg gcacaattca aagccactga
    24121 tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    24181 gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    24241 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    24301 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    24361 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    24421 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    24481 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    24541 aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg
    24601 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    24661 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    24721 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    24781 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    24841 attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa catactctac
    24901 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    24961 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    25021 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    25081 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    25141 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    25201 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    25261 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    25321 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    25381 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    25441 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    25501 caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    25561 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    25621 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    25681 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    25741 aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    25801 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    25861 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    25921 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    25981 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    26041 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    26101 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    26161 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    26221 cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc
    26281 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    26341 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    26401 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    26461 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    26521 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg
    26581 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    26641 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc
    26701 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    26761 gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac tttcagttgg
    26821 gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt gccgttacct
    26881 gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag tcgcgttata
    26941 ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac
    27001 tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg
    27061 ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac attcggtgca
    27121 cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc
    27181 aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct gttagatcca
    27241 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg
    27301 ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
    27361 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
    27421 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
    27481 tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc ccccggttgt
    27541 actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt
    27601 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcatcataag
    27661 ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca
    27721 cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt
    27781 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
    27841 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
    27901 caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc
    27961 cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg
    28021 ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt
    28081 aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa
    28141 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
    28201 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg agattccagt
    28261 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg tcaacattct
    28321 gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct gcaaccatcg
    28381 ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca gcttcacgtc
    28441 cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca
    28501 tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
    28561 gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt
    28621 tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata tcgcgcagct
    28681 cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg
    28741 cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
    28801 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
    28861 aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
    28921 ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
    28981 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
    29041 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
    29101 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
    29161 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
    29221 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
    29281 gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc ggataatcct
    29341 tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag gcctgcttga
    29401 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
    29461 tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa
    29521 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
    29581 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg
    29641 atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa aactcatcat
    29701 cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt
    29761 cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc
    29821 tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat
    29881 tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat
    29941 tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga
    30001 agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta
    30061 cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat
    30121 cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct
    30181 gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt
    30241 ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa
    30301 ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact tcgaaggcac
    30361 cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga
    30421 actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc tggagcagaa
    30481 agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa
    30541 agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct
    30601 ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa ccaacgaact
    30661 gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa
    30721 atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat
    30781 cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctccgg
    30841 tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac tggcatcggt
    30901 tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg
    30961 gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt
    31021 taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg gtgagcgcgg
    31081 tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc
    31141 aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc
    31201 gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat
    31261 cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga
    31321 agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa
    31381 gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta tccggcctac
    31441 aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg gtaagcgtcg
    31501 catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg gtctacaaca
    31561 gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa accttgttta
    31621 atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat tttatacctc
    31681 gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg caccagctcc
    31741 acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa ttcgatattg
    31801 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    31861 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    31921 aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc gtactttttg
    31981 cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt ggcgtatttc
    32041 actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc ggaatactgc
    32101 aatgcgattt catcaccgcc gaggtgtgaa aagacataat cgcggccata ttcccacgca
    32161 ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    32221 ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg aatatattcc
    32281 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    32341 ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc atcggcttct
    32401 tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt tttgatgccg
    32461 ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt gaaagcatga
    32521 atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg atccggcacc
    32581 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    32641 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    32701 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgccgggga
    32761 aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg gcttttaatt
    32821 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    32881 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    32941 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    33001 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    33061 cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg ggcaccgggc
    33121 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    33181 cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt tcggttacct
    33241 tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga ccaccgtgac
    33301 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    33361 gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    33421 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    33481 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    33541 gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa cgggagtcgg
    33601 catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc gggccagcca
    33661 gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc gttttggcga
    33721 aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    33781 catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt tgctcttctt
    33841 cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    33901 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccggtgacat
    33961 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc gtggatttcc
    34021 ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg cctcctagta
    34081 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    34141 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    34201 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    34261 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    34321 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatgacggg
    34381 caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    34441 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    34501 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    34561 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct ttgtactgcg
    34621 cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat
    34681 tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag aaagtaaagc
    34741 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agctgcgtta
    34801 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    34861 tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    34921 gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt tcatcaatac
    34981 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    35041 gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg cgtgtagccc
    35101 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    35161 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    35221 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    35281 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    35341 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    35401 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    35461 gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc tgttctgtct
    35521 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    35581 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    35641 tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca
    35701 gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    35761 cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg gtaaaaaagc
    35821 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    35881 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    35941 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    36001 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    36061 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    36121 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    36181 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    36241 tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt ggaaagagtg
    36301 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg gcggtggcgg
    36361 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    36421 cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    36481 cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc gcaaagtctc
    36541 aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct tgctactcaa
    36601 tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    36661 aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggctcac
    36721 catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcacgtg
    36781 gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt
    36841 gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc gcatctgctg
    36901 gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc
    36961 acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg
    37021 cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg
    37081 gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg
    37141 gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc tggaaaacca
    37201 ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg
    37261 tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc
    37321 tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga
    37381 aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa
    37441 atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat atgaaaaata
    37501 atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc
    37561 cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc
    37621 agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg
    37681 gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc
    37741 gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac gccgaggatg
    37801 ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag
    37861 aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac tgcgttacgc
    37921 accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat gcggttaact
    37981 ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac gtgcggcaga
    38041 actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt gatcagttct
    38101 ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg
    38161 cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct
    38221 tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg
    38281 tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt atcctttttg
    38341 tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg gctttgccat
    38401 attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt
    38461 cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc ggaatgtgtt
    38521 cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg gtgaggatca
    38581 ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg aacagttcca
    38641 gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg aagtcaacga
    38701 agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa
    38761 acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg gcgtagatgt
    38821 ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt ttctgcagca
    38881 cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc tggaagttac
    38941 cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag ccgccgttgg
    39001 tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg aaagtttctt
    39061 catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt
    39121 tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga
    39181 ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt
    39241 tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg atttcccagt
    39301 gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa
    39361 cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg
    39421 ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca ttatcagctt
    39481 cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc
    39541 gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt
    39601 tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt
    39661 cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca agtttaattg
    39721 ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg aggataaccg
    39781 ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta aagccgacgc
    39841 tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact actgccatcc
    39901 ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg
    39961 tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt
    40021 gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca
    40081 atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac atctccacgc
    40141 acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc aggaactgca
    40201 ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga ttcatgtcgg
    40261 tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc gcttcggcaa
    40321 gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca
    40381 ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga
    40441 tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca gccggagcca
    40501 gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt
    40561 tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag
    40621 tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct gcgaccatcg
    40681 gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta tcaacaatcg
    40741 ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg
    40801 tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg
    40861 caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt
    40921 catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg
    40981 taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac gccttatccg
    41041 gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc
    41101 ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc agcaggcttg
    41161 ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca
    41221 gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg
    41281 gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgag
    41341 cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac
    41401 ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg
    41461 tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca
    41521 ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg aacaccacgt
    41581 caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt
    41641 cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg gtgccctgct
    41701 ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata gcggcggtat
    41761 tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg gtgtcgtcgt
    41821 cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag
    41881 tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct
    41941 aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct acttacaagt
    42001 ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg
    42061 tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct
    42121 tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc
    42181 cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggcactg
    42241 gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg cgcgctggat
    42301 gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta
    42361 cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa
    42421 gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca
    42481 tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta
    42541 acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg
    42601 ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac ccgcgcgatc
    42661 ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc tgaaatcacc
    42721 cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa accgcacatc
    42781 gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat tgtgagctgt
    42841 tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa cctgaaagcc
    42901 attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc gcactactgc
    42961 cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca atcacacttc
    43021 tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt tgtgccaatg
    43081 tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga agggattgcc
    43141 gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag cgcgcaactg
    43201 gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc catgttacac
    43261 gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt gccgtatggc
    43321 gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc ttattattat
    43381 tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc gcaggcactg
    43441 gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc caatgtcagc
    43501 ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt agaaaaatat
    43561 cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc acgcaacact
    43621 cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg ctttagtcag
    43681 taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc tgtttttgca
    43741 gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac gttgcaatga
    43801 ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg acggcaaagt
    43861 ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt gtccgcaaca
    43921 tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg tgcgcaaagc
    43981 ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc aactgccgtt
    44041 tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc tcacctgtct
    44101 ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc cggtgtgtga
    44161 cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat taaccggacg
    44221 cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca agctggcgga
    44281 actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg aagaactggc
    44341 ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc atgcccacgg
    44401 cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg agccgttggc
    44461 tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct atttgtgaca
    44521 tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg cgtcaagcgt
    44581 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    44641 ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg cattatgcgc
    44701 cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac accgcatacg
    44761 tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac tcgcaaagca
    44821 cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa ccgttgagcg
    44881 gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat
    44941 cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca
    45001 gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca acaccagcac
    45061 gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc cgtgaatgca
    45121 ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt aaccactgct
    45181 gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca
    45241 ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca tcgctatgtt
    45301 tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa ttttccgcta
    45361 acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc
    45421 ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc gggaaatcaa
    45481 aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg
    45541 gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc
    45601 aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc
    45661 tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag
    45721 tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt
    45781 cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa attgctgaat
    45841 gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc
    45901 acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac
    45961 agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc
    46021 atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaaa
    46081 gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa
    46141 gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc
    46201 aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag
    46261 ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg
    46321 ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc
    46381 cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc
    46441 gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca
    46501 caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag
    46561 cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa agaaagacaa
    46621 agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga
    46681 taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac aaactcacta
    46741 ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca
    46801 gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac
    46861 gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc
    46921 actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac
    46981 gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
    47041 actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct gaaccacgcc
    47101 atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca gcagtttggc
    47161 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc cttctttagc
    47221 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt tcgccacttt
    47281 cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca cgactttttg
    47341 atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca gcgccgcctt
    47401 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
    47461 gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt aggcagagcc
    47521 ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct tcagtcttgt
    47581 catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca acacgaggtt
    47641 gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc catcacaaaa
    47701 gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat atcggtagaa
    47761 tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta acgccatcag
    47821 aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg atatcagcga
    47881 aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata aggaagggaa
    47941 attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc gcgggtctgg
    48001 tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat gctggtagcg
    48061 atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt acgcttgaac
    48121 tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac gttgactgac
    48181 tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac atcaaactgc
    48241 agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa cacctgcacc
    48301 gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt cagcggatct
    48361 ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt gaacttcatc
    48421 atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg caggccgagg
    48481 atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa ggtcagcgcg
    48541 tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc tttttcatat
    48601 ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc catgttggcc
    48661 tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt acgggtatcg
    48721 gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat atgatcaacg
    48781 ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc aaggaacagc
    48841 tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg cagctgctgt
    48901 gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt aacaatgcgg
    48961 acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc tttataaagg
    49021 tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg tgcgcagcca
    49081 attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat atcacggtca
    49141 agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc catcagctta
    49201 cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca gtccaccgcc
    49261 gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta
    49321 accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat cccacgctgc
    49381 ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc ttttactgta
    49441 ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt agttttaccg
    49501 gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt caaataagga
    49561 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
    49621 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
    49681 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
    49741 cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
    49801 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
    49861 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
    49921 agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg caaggcgcta
    49981 aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag cagagaaacc
    50041 gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc gttaagcaag
    50101 cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat
    50161 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
    50221 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
    50281 aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
    50341 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
    50401 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
    50461 gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt
    50521 tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga cgctgatagt
    50581 cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
    50641 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
    50701 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
    50761 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
    50821 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
    50881 gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
    50941 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
    51001 aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc gtttgccacc
    51061 atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa cgcgccaggg
    51121 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
    51181 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
    51241 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
    51301 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
    51361 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
    51421 cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac gcccgtttta
    51481 gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac tactggccga
    51541 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccagtgggga
    51601 cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag cagatgctgg
    51661 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc
    51721 gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac agcgtcgatg
    51781 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
    51841 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
    51901 gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
    51961 gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc aacggtgttg
    52021 aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt gatatgatca
    52081 tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
    52141 ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
    52201 tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt atcgcgcaaa
    52261 ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa ggttgagtat
    52321 ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc cgacctacaa
    52381 aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca tctggcatct
    52441 gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt tccagcaatt
    52501 acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg
    52561 tggtagcaat tctttttaag aattgatgg
//
LOCUS       DAJTYL010000012        49028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYL010000012 DAJTYL010000000
VERSION     DAJTYL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.012"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..718
                     /locus_tag="OO572_000723"
     CDS             <1..718
                     /locus_tag="OO572_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6659831.1"
                     /translation="GIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKG
                     IELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAG
                     LTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLD
                     AVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWN
                     EQAEKYLLAQFGVDIGEAAQ"
     gene            715..1680
                     /gene="yggF"
                     /locus_tag="OO572_000724"
     CDS             715..1680
                     /gene="yggF"
                     /locus_tag="OO572_000724"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6659832.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1702..2211
                     /gene="fumE"
                     /locus_tag="OO572_000725"
     CDS             1702..2211
                     /gene="fumE"
                     /locus_tag="OO572_000725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6659833.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2208..2921
                     /locus_tag="OO572_000726"
     CDS             2208..2921
                     /locus_tag="OO572_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6659834.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(2893..3570)
                     /locus_tag="OO572_000727"
     CDS             complement(2893..3570)
                     /locus_tag="OO572_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6659835.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3564..4241)
                     /locus_tag="OO572_000728"
     CDS             complement(3564..4241)
                     /locus_tag="OO572_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6659836.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4229..4612)
                     /locus_tag="OO572_000729"
     CDS             complement(4229..4612)
                     /locus_tag="OO572_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6659837.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4628..4936)
                     /locus_tag="OO572_000730"
     CDS             complement(4628..4936)
                     /locus_tag="OO572_000730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659838.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(4937..5515)
                     /locus_tag="OO572_000731"
     CDS             complement(4937..5515)
                     /locus_tag="OO572_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6659839.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5538..5969)
                     /locus_tag="OO572_000732"
                     /pseudo
     CDS             complement(5538..5969)
                     /locus_tag="OO572_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6341..7360
                     /gene="epd"
                     /locus_tag="OO572_000733"
     CDS             6341..7360
                     /gene="epd"
                     /locus_tag="OO572_000733"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6659840.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7410..8573
                     /gene="pgk"
                     /locus_tag="OO572_000734"
     CDS             7410..8573
                     /gene="pgk"
                     /locus_tag="OO572_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6659841.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8788..9867
                     /gene="fbaA"
                     /locus_tag="OO572_000735"
     CDS             8788..9867
                     /gene="fbaA"
                     /locus_tag="OO572_000735"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6659842.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10058..10918
                     /gene="mscS"
                     /locus_tag="OO572_000736"
     CDS             10058..10918
                     /gene="mscS"
                     /locus_tag="OO572_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6659843.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11057..11692
                     /gene="argO"
                     /locus_tag="OO572_000737"
     CDS             11057..11692
                     /gene="argO"
                     /locus_tag="OO572_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6659844.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11785..12525
                     /locus_tag="OO572_000738"
     CDS             11785..12525
                     /locus_tag="OO572_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6659845.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12702..13652
                     /locus_tag="OO572_000739"
     CDS             12702..13652
                     /locus_tag="OO572_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6659846.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13594..15072)
                     /gene="scpC"
                     /locus_tag="OO572_000740"
                     /pseudo
     CDS             complement(13594..15072)
                     /gene="scpC"
                     /locus_tag="OO572_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15096..15881)
                     /gene="scpB"
                     /locus_tag="OO572_000741"
     CDS             complement(15096..15881)
                     /gene="scpB"
                     /locus_tag="OO572_000741"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6659847.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15892..16887)
                     /gene="meaB"
                     /locus_tag="OO572_000742"
     CDS             complement(15892..16887)
                     /gene="meaB"
                     /locus_tag="OO572_000742"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6659848.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16880..19024)
                     /gene="scpA"
                     /locus_tag="OO572_000743"
     CDS             complement(16880..19024)
                     /gene="scpA"
                     /locus_tag="OO572_000743"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6659849.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19228..20121)
                     /gene="argP"
                     /locus_tag="OO572_000744"
     CDS             complement(19228..20121)
                     /gene="argP"
                     /locus_tag="OO572_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6659850.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20504..21163
                     /gene="rpiA"
                     /locus_tag="OO572_000745"
     CDS             20504..21163
                     /gene="rpiA"
                     /locus_tag="OO572_000745"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6659851.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21419..22651
                     /gene="serA"
                     /locus_tag="OO572_000746"
     CDS             21419..22651
                     /gene="serA"
                     /locus_tag="OO572_000746"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6659852.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22880..22939
                     /gene="ibsC"
                     /locus_tag="OO572_000747"
     CDS             22880..22939
                     /gene="ibsC"
                     /locus_tag="OO572_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6659853.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23272..23331
                     /locus_tag="OO572_000748"
     CDS             23272..23331
                     /locus_tag="OO572_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6659854.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23432..24034)
                     /locus_tag="OO572_000749"
     CDS             complement(23432..24034)
                     /locus_tag="OO572_000749"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6659855.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24055..24238)
                     /gene="ssrS"
                     /locus_tag="OO572_000750"
     ncRNA           complement(24055..24238)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO572_000750"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24280..24609)
                     /gene="zapA"
                     /locus_tag="OO572_000751"
     CDS             complement(24280..24609)
                     /gene="zapA"
                     /locus_tag="OO572_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6659856.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24777..25355
                     /gene="ygfB"
                     /locus_tag="OO572_000752"
     CDS             24777..25355
                     /gene="ygfB"
                     /locus_tag="OO572_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6659857.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25381..26706
                     /gene="pepP"
                     /locus_tag="OO572_000753"
     CDS             25381..26706
                     /gene="pepP"
                     /locus_tag="OO572_000753"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6659858.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26703..27881
                     /gene="ubiH"
                     /locus_tag="OO572_000754"
                     /gene_synonym="visB"
     CDS             26703..27881
                     /gene="ubiH"
                     /locus_tag="OO572_000754"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6659859.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27904..29106
                     /gene="ubiI"
                     /locus_tag="OO572_000755"
                     /gene_synonym="visC"
     CDS             27904..29106
                     /gene="ubiI"
                     /locus_tag="OO572_000755"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6659860.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29180..29263)
                     /locus_tag="OO572_000756"
     CDS             complement(29180..29263)
                     /locus_tag="OO572_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659861.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29554..30648
                     /gene="gcvT"
                     /locus_tag="OO572_000757"
     CDS             29554..30648
                     /gene="gcvT"
                     /locus_tag="OO572_000757"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6659862.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30672..31061
                     /gene="gcvH"
                     /locus_tag="OO572_000758"
     CDS             30672..31061
                     /gene="gcvH"
                     /locus_tag="OO572_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6659863.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31180..34053
                     /gene="gcvP"
                     /locus_tag="OO572_000759"
     CDS             31180..34053
                     /gene="gcvP"
                     /locus_tag="OO572_000759"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6659864.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34320..35063
                     /locus_tag="OO572_000760"
     CDS             34320..35063
                     /locus_tag="OO572_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6659865.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35120..36553)
                     /gene="bglA"
                     /locus_tag="OO572_000761"
     CDS             complement(35120..36553)
                     /gene="bglA"
                     /locus_tag="OO572_000761"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6659866.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36598..36908
                     /locus_tag="OO572_000762"
                     /pseudo
     CDS             36598..36908
                     /locus_tag="OO572_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37072..37731
                     /locus_tag="OO572_000763"
     CDS             37072..37731
                     /locus_tag="OO572_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6659867.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37927..38907)
                     /gene="ygfZ"
                     /locus_tag="OO572_000764"
     CDS             complement(37927..38907)
                     /gene="ygfZ"
                     /locus_tag="OO572_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6659868.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39150..39416
                     /gene="sdhE"
                     /locus_tag="OO572_000765"
     CDS             39150..39416
                     /gene="sdhE"
                     /locus_tag="OO572_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6659869.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39397..39804
                     /locus_tag="OO572_000766"
     CDS             39397..39804
                     /locus_tag="OO572_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6659870.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39844..40365)
                     /gene="fldB"
                     /locus_tag="OO572_000767"
     CDS             complement(39844..40365)
                     /gene="fldB"
                     /locus_tag="OO572_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6659871.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40477..41373
                     /gene="xerD"
                     /locus_tag="OO572_000768"
     CDS             40477..41373
                     /gene="xerD"
                     /locus_tag="OO572_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6659872.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41398..42108
                     /gene="dsbC"
                     /locus_tag="OO572_000769"
     CDS             41398..42108
                     /gene="dsbC"
                     /locus_tag="OO572_000769"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6659873.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42114..43847
                     /gene="recJ"
                     /locus_tag="OO572_000770"
     CDS             42114..43847
                     /gene="recJ"
                     /locus_tag="OO572_000770"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6659874.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43938..45036
                     /gene="prfB"
                     /locus_tag="OO572_000771"
     CDS             join(43938..44012,44014..45036)
                     /gene="prfB"
                     /locus_tag="OO572_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6659875.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45046..46563
                     /gene="lysS"
                     /locus_tag="OO572_000772"
     CDS             45046..46563
                     /gene="lysS"
                     /locus_tag="OO572_000772"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6659876.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46606..47154)
                     /gene="idi"
                     /locus_tag="OO572_000773"
     CDS             complement(46606..47154)
                     /gene="idi"
                     /locus_tag="OO572_000773"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6659877.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47209..47280
                     /locus_tag="OO572_000774"
     CDS             47209..47280
                     /locus_tag="OO572_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659878.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47277..47402)
                     /locus_tag="OO572_000775"
     CDS             complement(47277..47402)
                     /locus_tag="OO572_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659879.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47404..48852)
                     /gene="uacT"
                     /locus_tag="OO572_000776"
     CDS             complement(47404..48852)
                     /gene="uacT"
                     /locus_tag="OO572_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6659880.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11832 a        12292 c        13217 g        11687 t
ORIGIN      
        1 gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt
       61 ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc
      121 ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctatcca
      181 gatgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt
      241 gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt
      301 tgccgggctg acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa
      361 cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc
      421 ccttagcgcc acaggacagt tacagccgtc gtttatggtg acccatattg gtggcctgga
      481 tgcggtgcca gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta
      541 taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga
      601 tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca
      661 ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg
      721 tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa
      781 accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca
      841 ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg
      901 ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg
      961 gttgatccca ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc
     1021 atggctttcg ctcctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg
     1081 gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc ggacaacttg
     1141 cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat
     1201 aaaccgcgtc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc
     1261 ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat
     1321 gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg
     1381 ttaggcgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg
     1441 gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt
     1501 aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg
     1561 ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg
     1621 cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga
     1681 tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg
     1741 gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg
     1801 atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca
     1861 gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg
     1921 atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca cttttcccag
     1981 tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga tataacctgg
     2041 gattttatca gcaatgtcaa cagtataact cgcaatgcga cgctctacga tgcgttaaaa
     2101 gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa
     2161 acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat
     2221 taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc
     2281 ataaactgtt gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg
     2341 tggtttttct ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat
     2401 atctcgcgca acaagacccg gagctgcccg ctattcaggc gctcccgatg gatggttttc
     2461 accattacaa tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga
     2521 catttgacgt tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt
     2581 ggccgcagta cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac
     2641 cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg
     2701 cgcaattttg tgatttctcg atctttatca acgcgccagc tgctgcgttg cgggagcgtc
     2761 tggtggggcg caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc
     2821 gtaccgacgg gccgaatgtt cgccgggggc tggaagagag tctgcctgca aatttgacct
     2881 taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc
     2941 ttccagccgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc
     3001 taccacgctc gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca
     3061 attttcgtca aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc
     3121 caggcaggcg acggcgacca tccgtcgctg cgcattatgc agatccaacg ggtgcgagtt
     3181 ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc
     3241 cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa
     3301 aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt
     3361 caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg taagaagacc
     3421 tgccattact cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca
     3481 ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca
     3541 gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc
     3601 cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc
     3661 gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggt
     3721 gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag
     3781 gcgatgatca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac
     3841 ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca
     3901 agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag
     3961 agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg
     4021 agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag
     4081 tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca
     4141 aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt
     4201 gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg
     4261 tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg
     4321 cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac
     4381 ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac
     4441 gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa
     4501 taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag
     4561 cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt
     4621 actgcattca caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag
     4681 catgcgacca ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac
     4741 cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc
     4801 gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca
     4861 aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc taatgcccac agtgttaatg
     4921 aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa
     4981 tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt
     5041 tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc
     5101 aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg
     5161 gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca
     5221 gggtgcgaaa ccaacccgct cgcgccagcc aaccggagac caggccgatg accatcgcga
     5281 cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta
     5341 agccggtcag cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg
     5401 agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg
     5461 cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa
     5521 aacctctttg aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc
     5581 gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg
     5641 gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa
     5701 acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg
     5761 aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc
     5821 aggctaccgg tacagcctgc aatctaggcg gcatgaagtt gatgttgctg tgcaaaagcg
     5881 tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag
     5941 gaagaatatg agtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg
     6001 ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac
     6061 gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg
     6121 gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt
     6181 tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta
     6241 accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct
     6301 gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat
     6361 aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg
     6421 ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt
     6481 gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca
     6541 actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc
     6601 ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga
     6661 gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag
     6721 taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca
     6781 ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt
     6841 agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga
     6901 tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc
     6961 gatcattccg gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa
     7021 cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt
     7081 aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc
     7141 agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga
     7201 ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc
     7261 acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct
     7321 cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa
     7381 aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc
     7441 ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag
     7501 taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag
     7561 gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag
     7621 aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc
     7681 tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa
     7741 acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg
     7801 cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta
     7861 ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac
     7921 tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg
     7981 gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc
     8041 tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta
     8101 aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca
     8161 tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc
     8221 tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg
     8281 ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg
     8341 gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaac gctatcgcag
     8401 acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg
     8461 gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg
     8521 aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca
     8581 cagggcaggg aaacctgcac ttgtttcagc gcgcttttag agcacgcatt ttttcatact
     8641 ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccccaggag cttacataag
     8701 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat
     8761 aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc
     8821 gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca
     8881 ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct
     8941 aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt
     9001 aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg
     9061 catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac
     9121 tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc
     9181 gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg
     9241 caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact
     9301 ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg
     9361 gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc
     9421 aaaatcagcc cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag
     9481 ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag
     9541 aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact
     9601 gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaacat cgataccgat
     9661 acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg
     9721 cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg
     9781 cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag
     9841 gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc
     9901 tgcgggtctt tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg
     9961 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    10021 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    10081 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    10141 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    10201 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    10261 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg
    10321 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    10381 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    10441 cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    10501 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    10561 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    10621 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    10681 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    10741 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    10801 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    10861 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    10921 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    10981 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    11041 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat
    11101 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    11161 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    11221 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctagcgctgg tcacctgggg
    11281 cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    11341 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    11401 gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag
    11461 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    11521 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    11581 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    11641 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    11701 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa
    11761 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg
    11821 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    11881 tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac
    11941 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    12001 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    12061 acccagccag attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg
    12121 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    12181 ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc
    12241 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    12301 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    12361 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    12421 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    12481 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    12541 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    12601 ttttctgtaa aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc
    12661 tgactgaaac ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga
    12721 aaaagatgcg caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa
    12781 aaatccatat gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg
    12841 gctatacgct atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac
    12901 tgtatcaaaa actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata
    12961 actcagggcg tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta
    13021 ttttcgatca gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg
    13081 agttcagcga aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct
    13141 ccccacagca tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac
    13201 caccgctgcg attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc
    13261 tgcttcagga gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa
    13321 tgattgatgc gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca
    13381 gttttatggc caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt
    13441 ttgcctggcg actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga
    13501 tgtatatgca ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatcttca
    13561 gcatattgct gaagatcgtg acgggacaaa ccattaaccc agcatcgagc cggttgcaat
    13621 taaattacgg tgtaagtcga agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc
    13681 attttccaga tagcgatgca gataatcccg atacatagga tgtgcacaat tatcaatgat
    13741 agtgcgggcg cgttgaagcg gggaaagacc gcgcagatcg gcgatccctt gttcggtgat
    13801 gatcactttg acgctgtgtt cgctgtgatc aacatggctg cacattggca cgacggttga
    13861 gatcttgcct tctttagcaa tcgacggagc cataaagatc gacagatacg cgttgcgttc
    13921 aaaatcaccg ctgccgccga tgccgttcat cagatcgacc ccggctacgt gcgttgaatt
    13981 tgcatgcccg taaatatcaa actccaggcc gacgttcaga gcgatgacgc ccagacgacg
    14041 gatgatttcc gggttattgg aaatctcctg cggacgcaac acaatgcggc tggcaaagta
    14101 atccatattg tcgtaaatct tgcgcaggga atcggccgag attgtcaggc tggaggcgct
    14161 ggccccgctg attttgccgg tttccagtaa atgcaccacc gattcctgta gcacttccga
    14221 atacatcatg aacggaggaa tttccgggtt ttcccccaga cgtgccatta ccgcattatt
    14281 gatattgccc acgccacttt gcagcggcag aaattccggc ggaatacgcc catgcgccat
    14341 ttcctgcaat aagaacgtga ccacgttatc ggcaatctgc tggcacatgg gattttgctt
    14401 atccagcata ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa tctttttcgg
    14461 atcgatttgc acatagcggg taccgacgcg atccattgca tggaagatcg acacgctatt
    14521 gcgccgcggt ggcgcgccag gaatcacaat atctgccagt tctgcaacgc gcggatcgtg
    14581 atagtgatta agttcaatga tcactttctt cgctcgcagc agctaggtcg gcgcattacc
    14641 gatcccgctg gttaaccaga ctcgaccatc cggtgccagt gccgatgctt caatgacggc
    14701 aacatcaatg tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag
    14761 gtcaacgaaa ctcaccgcgc cctgattgat ctttttacgt aaactggacg atgtttgata
    14821 tggcgcacgc caggaaacag catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat
    14881 tgacgccccc gtcagcaggc gaatttgata cggctttttg gcctcatgct gttcgttagc
    14941 tctgcgggca atcgcggcgg gtagggcttt cggcgaaccc gctggggtaa agccgctaaa
    15001 tgccaccatg tcgttatgct ggataatttc tgccgcttca tcggcggtca tccttgtcca
    15061 ctgagtttcc atttttactc cttcgttcgc agggattaat gaccaacgaa attaggttta
    15121 cgtttttcga ggaaagcgtt catcccttcc tggtaatctt cgctgtcata aaccgcgcgg
    15181 cgcatcccct gaatacgttc aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca
    15241 cgcagctctt ctttgataac ggcaatggct aacggcgctt tctcagagat gtggtgcgcc
    15301 atttgtaagg tgaaatcttc cagttcttcc acttccacaa catggttgag gatgccgaca
    15361 gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg
    15421 aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac gccgaggttt
    15481 acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga actcatgatc
    15541 atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg tttcgggaat
    15601 ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg
    15661 ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc acttggcgcc
    15721 cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg cataagatca
    15781 tcaataaaga ctttacttaa ggcattaagt tttcgtccat agttaaactc aatgactgcc
    15841 actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc tttaatcaaa
    15901 atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt
    15961 gtttttgacc gctaaaagcg tctggcggta atagcgatcg aaatcttcat tcgcgaacag
    16021 gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt gttgttgccg
    16081 cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga tggcgtgcca
    16141 gatctcatcg attccacgtt tttccagtgc gctacaagtc agaccccgtg gctgccattc
    16201 gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg caatggcgac
    16261 attggtatgg ttatcgccat cgtctttgtt gataacgagc agatcagcca cttccatcag
    16321 ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg agataaaaca
    16381 gtccaccatg cgggcgactt ccgtttccga ctgcgcgatg ccaaccgttt cgacaatcac
    16441 tacgtcataa cccgccgctt cgcataacag cattaattcc cgtgctcgct gactggcacc
    16501 gcccagatga ccggaggagg gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc
    16561 attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc tgggatcgac
    16621 cgcaataaca gcaaccttta atccctcacg aatcaacaac atgccaaagg cttcaagaaa
    16681 ggtacttttc cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt taccacagaa
    16741 cggcataata gcgtcaagca gctgtgtact tagcgcctga tgacgtgggt gacggctttc
    16801 caccagcgtc atggcttggg cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact
    16861 ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt acgtcgcgca
    16921 cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc tcttgcagga
    16981 aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct tcgcgtcccc
    17041 attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca gccagtgagg
    17101 atgcgcccac tacgtgaacg tcgttttcta cggctaggcg ggcgatctct tcaggtgtag
    17161 agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg gcgatcactt
    17221 tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc gggcgacgac
    17281 cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg gaggccgatt
    17341 tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt ggcaccagat
    17401 aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag
    17461 cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt
    17521 tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa
    17581 tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg tcttcgtgat
    17641 ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt
    17701 cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc gctttcgcca
    17761 tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg atttgatcgg
    17821 tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgtggcag agttctgatt
    17881 cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac
    17941 caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc gtcgcagcca
    18001 gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc aggctccagc
    18061 ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac
    18121 tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc ataaacagat
    18181 ccatgccgat accgaagaat aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg
    18241 cagagattgc tgctttgatg tactcaatcc catcggcgag cgtaaatgct acctgctgca
    18301 cgcagttggc acccgcttcc cccatgtggt aaccgctgat actgatggta ttaaatcgcg
    18361 gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac ggttttggtg
    18421 ggtaaatata ggtgttgcgg cagaggtact ctttgagaat atcgttttga atggtgccgg
    18481 tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac gatataaaac gccagtactg
    18541 gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg atctggtcga
    18601 acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg acgtcacccg
    18661 ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa
    18721 gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct tttgctgttg
    18781 aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg gcacgcgggc
    18841 cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta tcgagatcgg
    18901 cttcggtata cagcggcttg atggcgatcc cttccgcggt ttgctgaacc agcgagtcga
    18961 cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc tgctcgttag
    19021 acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt
    19081 ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt gccgcctgag
    19141 atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac aacgcctgat
    19201 atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt gaccataatc
    19261 gagtaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc ggtgccagta
    19321 gagcatccgt cgttgaaata gcccaggcgt taagtcaatc agttcaccgc tggccagctc
    19381 tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag caagttgtac
    19441 gaacgcttct gaagaattaa cgatatggca gggtacgctg ccaggaggaa gatcgaagtt
    19501 ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aacgcgacca ctggcgcttt
    19561 caataatgcc gaacgcgtta cgccgttagg aaaatatttt tcggcaaacg gttttgagct
    19621 gacgaacaga tagtcgagcg caccgagttt atcgacaaga caactcggca gcgcctgatg
    19681 ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag tacgggtttc
    19741 atcttctacc tgcaagttga ggcggatagg cgaatcagcc aacacaggag ccagtgccgg
    19801 aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga
    19861 accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc gcagcagtgc
    19921 cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca acagcggctg
    19981 cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg taatgcacag
    20041 cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg cctgtaatgt
    20101 tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact
    20161 atgacacaat tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa catttgtatc
    20221 aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt cgcttagcga
    20281 cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc accgtggagc
    20341 ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc cgccattgcg
    20401 cgatatttta tggatgagtt aaccacccgg cttgccaacg gggtctgaat cgcttttttt
    20461 gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt
    20521 gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggtacca ttgttggtgt
    20581 aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga aaggccagat
    20641 tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca
    20701 cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat
    20761 caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat
    20821 tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg ttgatattct
    20881 gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca
    20941 gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataatggcaa
    21001 cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg aaaacgccat
    21061 aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc
    21121 gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc
    21181 ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgctt ttaccaggca
    21241 attgtcgatt gctctaaata aatcctctaa accagcatat tcatccaaga attacctttg
    21301 cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat tattttttga
    21361 tatgttgaaa ggcggatgca aatccgcaca caacatttca aaagacagga ttgggtaaat
    21421 ggcaaaggta tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca
    21481 aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc
    21541 gctggatgat gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc
    21601 ccgtacccat ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg
    21661 tttctgtatc ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt
    21721 atttaacgca ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct
    21781 gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa
    21841 actggcggcg ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca
    21901 tattggtacg caattgggca ttctggctga atcgctggga atgtatgttt acttttatga
    21961 tattgaaaac aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct
    22021 gaatatgagc gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat
    22081 gggcgcgaaa gagatttcgc taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg
    22141 tactgtggtg gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc
    22201 ggcaatcgac gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact
    22261 gtgtgaattc gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca
    22321 ggagaatatc ggcttggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac
    22381 gctctctgcg gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat
    22441 gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca
    22501 gggcgtcaac atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat
    22561 tgatattgaa gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc
    22621 gggtaccatt cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg
    22681 tcactgactt gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac
    22741 tttgcgagtc tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat
    22801 ggggatttac gatggcggga cagcatgggg ctgtaacggt aaagccctca ccgaagcgag
    22861 ggcttaaagg agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat
    22921 aagtttcgga gcttattaac agtcagtctc aagggaggag tcatcctccc ttacccttac
    22981 tcactaaatt aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc
    23041 tgaaccgaac tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt
    23101 ttcccaaaaa ttatctctgt tttgcatggg gtttttcgag cacgctcgaa gaaaagattt
    23161 aaaggtagat caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg
    23221 ggctgtaacg gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga
    23281 cttgccatca tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc
    23341 acaggggagg agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga
    23401 tgtcaatcag ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa
    23461 ccaccgcagg aagagggata tcccactctt caacggggag tttttccacc aactgacaat
    23521 catgcgcata acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat
    23581 aaaaaccacc gcccattccc agacgctgac cgtactcatc aaaggcgacc agcggtgtga
    23641 tcagcacgtc taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga
    23701 tcttcaacct gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac
    23761 cggcactaaa gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt
    23821 ctattagcgg ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg
    23881 ccatcaccac cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttccagct
    23941 gttgttccgg cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg
    24001 ataatgtcag tgggagctct ggtagttgcg tcatggctgg tgccagataa gaagggaatc
    24061 tccgagatgc cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc
    24121 gcagttttaa ggcttctcgg acgaaccgag catgctcacc aaccgcggag cgccacattc
    24181 ttgtggtatg aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt
    24241 tgtcttcacg gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt
    24301 tttttcggtg atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg
    24361 aatacgctgt tccatacttg ccgcgtagtc acgagtcttt gctttttctt gcgctaactc
    24421 atagctgata ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg
    24481 ttctttcaga tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct
    24541 ttggtcaggc gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg
    24601 tgcagacatg ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc
    24661 tgcgaagggg cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac
    24721 cagggcccaa agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt
    24781 ctatacagaa cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga
    24841 cgggtctgac ccctgccgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg
    24901 atgacagctc atggctgccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc
    24961 atgagctggc acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg
    25021 acggcttcct ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg
    25081 ctgatgcact ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga
    25141 agctggacaa agtgaccggc gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc
    25201 agctgggcta cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca
    25261 tcgaatacgt tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga
    25321 ccgcgccaga agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt
    25381 atgagtgaga tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg
    25441 caacccggca gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc
    25501 gaatacccct atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa
    25561 gcggtgctgg tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc
    25621 gttcgcgacc tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca
    25681 gagaaactgg gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa
    25741 ctacttaacg gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa
    25801 atcgtgaaca gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg
    25861 gcaacgatga tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa
    25921 gagattgccg tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg
    25981 gaaaaatgcc gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt
    26041 aaccgccacg gtgcgcgcta tccttcctat aacaccattg taggcagcgg tgaaaacggc
    26101 tgcattctgc actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt
    26161 gacgcgggtt gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac
    26221 ggcaaattca cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc
    26281 agcctgcgcc tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc
    26341 atcatggtta gcggcctggt aaaactcggc atcctgaaag gtaatgttga tgaactgatc
    26401 gctcagaacg cccatcgtcc tttctttatg catggcctta gccactggtt aggactggat
    26461 gtccatgacg ttggcgttta tggtcaggat cgctcgcgca ttctggaacc ggggatggta
    26521 ctgaccgtag agcctggtct gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc
    26581 ggtatcggca ttcgtattga agacgacatt gtgattaccg aaaccggcaa cgaaaacctc
    26641 accgccagcg tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag
    26701 caatgagcgt aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt
    26761 cccggttaag tcacggggcg ctgccggtac atttgattga agcgactgcg ccagagtcac
    26821 atgctcatcc gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac
    26881 tggcgcgcat cggcgtctgg caatctctgg ctgattgcgc aaccgccatc actaccgtgc
    26941 atgtcagcga tcgtggtcat gctggattcg tcaccctcgc cgcagaagat taccaactgg
    27001 cggcgctggg gcaggttgtc gaattgcata atgtcgggca acggctgttt gcattgctgc
    27061 gaaaagcacc tggcgtaatg ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc
    27121 agagtcatgt tgaagtgacg ctggagagcg gcgagatgct gacgggccgc gtgctgatag
    27181 ccgctgatgg cacccattca gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc
    27241 cttacgaaca actggcagtg attgccaacg ttgctacttc tgttgcgcac gaaggccgcg
    27301 cttttgaacg cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct
    27361 gttcgctggt ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg
    27421 agaagttttg ccgtgaactc cagtctgcct ttggctggcg actggggcaa attacccacg
    27481 ccggtaaacg cagtgcttat ccgctggcat taacccgcgc cgccagaccg attacccatc
    27541 gcactgtgct ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta
    27601 acctcggtat gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg
    27661 gagaagacat cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc
    27721 gcgaagcaac catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac
    27781 cgctggttgt cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg
    27841 atgtgctggc gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa
    27901 acaatgcaaa gtgttgatgt agccattgtt ggcggcggca tggtggggct ggcggttgcc
    27961 tgcggcttac aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct
    28021 ctggcggtag atgcactacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa
    28081 ttactcaccc gtcttggcgt ctggcaggac attctcgctc gtagggccag ttgttatcac
    28141 ggcatggaag tgtgggacaa agacagtttt ggtcacatct cgtttgacga tcaaagcatg
    28201 ggctatagcc atcttgggca catcgttgaa aactcagtga ttcactacgc gctgtggaac
    28261 aaagcgcagc agtcgtcaga tattactctg ttggccccca cagaattaca gcaggtcgcc
    28321 tggggagaaa atgaaacctt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg
    28381 gtgattggcg cggacggtgc taattcctgg ttgcgcaaca aagcagatat tccgctgact
    28441 ttctgggatt atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat
    28501 gcggtggcgc ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat
    28561 ccgcatcttt gctcgattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag
    28621 gcaagtgaag acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg ccttggctta
    28681 tgcaaggttg agagcgcgcg tcaggtgttc ccgctgacgg ggcgttatgc gcgccagttt
    28741 gccgcacacc gtctggcgct ggtgggcgac gccgcgcata ccattcaccc actggcgggg
    28801 cagggcgtta atctcggatt tatggacgcc gcagagctga ttgctgaact gaaacggttg
    28861 catcgtcagg gtaaagacat cgggcagtac atttacctgc gccgctatga acgtagccgc
    28921 aagcacagcg cggcgctgat gctggctggt atgcagggtt ttcgcgatct cttttccggc
    28981 gctaatccgg cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct
    29041 ggcgttaagc cgcaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg
    29101 cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat ctcccggttt
    29161 tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat
    29221 attttctaat accatgattt tttgatttag tgttttttga cattttttta gctcttaata
    29281 ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaatt ggtgtatttt
    29341 ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat gtgatgatca
    29401 attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg
    29461 cgttggggac cgggagtggc tccgatgctg ggttttgtgg tgataatttc accatgaaaa
    29521 agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa
    29581 caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctgcat
    29641 tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg
    29701 tcgcatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct gcgttatctg
    29761 ctggcgaacg atgtggcgaa gctcaccaaa agcggcaaag ccctttactc ggggatgttg
    29821 aatgcctctg gcggtgtgat agatgatctc atcgtctact actttactga agatttcttc
    29881 cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct
    29941 gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg
    30001 ccgaatgctc aggcaaaagc tgccacactg tttaatgatg ctcagcgtca ggcggtggaa
    30061 gggatgaaac cgttctttgg cgtgcaggtg ggcgatctgt ttattgccac cactggttat
    30121 accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga tttctggcgt
    30181 gcgctggtgg aagcgggcgt taagccctgc ggtctggggg cgcgtgacac gctgcgtctg
    30241 gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc tttagccgcc
    30301 aacatgggct ggactatcgc ctgggaaccg acagatcgtg actttatcgg tcgtgaagcc
    30361 ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat gaccgaaaaa
    30421 ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa
    30481 ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc gctggcgcgc
    30541 gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt
    30601 aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg
    30661 gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggc
    30721 tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct caggagctgt
    30781 taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg
    30841 actgcgcggt tgccgaatcg gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg
    30901 aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac agcgaaccgt
    30961 atgcaggcgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg gaatcactgc
    31021 tggatgcgac cgcatacgaa gcattgttag aagatgagta acggctttat tcctcttctg
    31081 cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc gtaaggagag
    31141 aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag
    31201 cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa
    31261 gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg
    31321 aaagatattc agcttgcgac accaccgcag gttggcgcac cggcgaccga atacgccgca
    31381 ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg
    31441 ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc
    31501 tggtataccg cgtacactcc gtatcagcct gaagtctccc agggccgcct tgaagcactg
    31561 ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc tgcttctctt
    31621 ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg
    31681 aaaaatgcca accgcttctt cgtggcttcc aatgtgcatc cgcaaacgct ggatgtggtc
    31741 cgtactcgtg ctgaaacctt tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc
    31801 gaccatcagg acgtcttcgg cgtgctgtta cagcaggtag gcactaccgg tgaaattcac
    31861 gactacactg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc
    31921 gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga tattgttttt
    31981 ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc ggcattcttt
    32041 gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt atcgaaagat
    32101 gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt
    32161 gagaaagcga actccaacat ttgtacttcc caggtactgc tggcaaacat cgccagcctg
    32221 tatgccgttt atcacggccc ggttggcctg aaacgtatcg ctaaccgcat tcaccgtctg
    32281 accgatattc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca tgcgcactat
    32341 ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg tgccgaagcg
    32401 gctgaaatca acctgcgtag cgatatcctg aacgccgtgg ggatcaccct tgatgaaacc
    32461 accacgcgtg aaaacgtaat gcagcttttc agtgtgctgc tgggcgataa ccacgggctg
    32521 gagatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca gcctgcgatg
    32581 ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctatcacag cgaaaccgaa
    32641 atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca ggcgatgatc
    32701 ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc aatcacttgg
    32761 acggaatttg ccgaactgca cccgttttgc ccgccggaac aggctgaagg ttatcagcag
    32821 atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc cgtttgtatg
    32881 cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg tcattatcat
    32941 gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc gcacggaact
    33001 aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac
    33061 ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga taacctctcc
    33121 tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg
    33181 tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa catgaacgcc
    33241 caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct caacctgcat
    33301 aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa
    33361 gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg catgttaacc
    33421 cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg
    33481 atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc
    33541 aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta taccggtcgc
    33601 gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga agaaaccggc
    33661 atcagcgagc tggacattgc caagcgcctg atcgactacg gtttccacgc gccgacgatg
    33721 tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag caaagtggaa
    33781 ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaattgacca ggtgaaagcc
    33841 ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca gagcgaactg
    33901 gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg tattcccggc aggcgtggca
    33961 gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg taacctgttc
    34021 tgctcctgcg taccgattag cgaataccag taattcactg attcgagtat tttctaaagg
    34081 cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca gtcccctcgc
    34141 ccctttgggg agagggttag ggtgagggga acaggccggc actggcgcga atatttaccc
    34201 tcaccccgac cctctccctg aaagggcgag ggggaaaagc gtgccaacat tgaagattga
    34261 gccagtttgt tagcaatctc aaagatacgt caacgaatta atttttctcg gaaaaacaaa
    34321 tggctatagc acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact gcattactgt
    34381 tggcgcaaga agggtatacg gtggcggtta attatcagca aaacctccac gcggcgcagg
    34441 aagtgatgaa cttaataatg caagccggtg gcaaagcatt cgtgctccag gcggatatca
    34501 gcgacgaaaa ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat gaaccgctag
    34561 cagcgctggt caataacgcc gggatcttgt ttacccagtg caccgttgaa aaccttaccg
    34621 cagagcgaat caaccgagta ctttccacca acgtgacggg atattttctc tgctgccgcg
    34681 aggcggtaaa acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct
    34741 cttcggtggc ctcacggttg ggttcgccag gggaatatgt tgattacgcg gcatcgaaag
    34801 gggcgattga ttcgttaacc accggactat cgctggaagt cgccgcgcag gggatccgcg
    34861 ttaactgcgt gcggccaggg tttatttata ccgaaatgca cgccagcggc ggcgagcctg
    34921 gacgcgtcga tcgcgttaag tcgaacatcc ccatgcagcg tggtggacag gcagaagagg
    34981 tcgcgcaggc cattgtctgg ctactaagtg ataaagcctc ttacgtcacg ggaagtttta
    35041 tcgatttggc gggcgggaaa taaaacaggg aagttgtctg accggatgca acaagtattg
    35101 catccggtac ttcatcgact taaagcttct cgccgttgct ggcaatcacc tctttgtacc
    35161 agttaaagct cttcttacgt gaacgcgaca tatcgccagt accgtcgtca tgtttattca
    35221 catagataaa gccgtagcgt ttgctgtact gcccggtagt gaatgacacg cagtcgatgc
    35281 agccccacgg tgtgtagccc atcagatcca caccatcgta agtcactgct tttttcatct
    35341 cttcaatatg ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt
    35401 ccactttgtc gtaagcgcca aaaccgtttt cgacaataaa cagcggcctc tgataacgct
    35461 catacagttc gcaaagggca tagcgcagac ctactggatc aatctgccag ccccagtcag
    35521 acgctttaac atacgggttc ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc
    35581 cgccttcggc cttcactgcg ttggtcatgt aatagctgaa accaagataa tcgcaggtgc
    35641 cttcgcgcag tacatcgaga tcgccgtctt ccattttgat attaaatccg cgacgttccc
    35701 actcgttcaa tacataggac gggtaatagc cgcatagctg cacatcggta aagacgtagc
    35761 gttcgcgcat cgactcctga gcgaacatca catcgtccgg gttacatgag tagggataga
    35821 gcggcaccat cgccagcata cagccgactt tcatctccgg gttaatgcga cgcgcggctt
    35881 tcactgccag ggcgctggcg acaaactggt gatgcagcac ctggtacatc gtctcttccg
    35941 ggttgtcatg ctcggtatac accacgccgg agcagcagta accaaacagc ggcgcacgcc
    36001 agttacgctg gttgtttatt tcgttgaagg tcatccagta tttgacttta tgcttgtagc
    36061 gttcgaatac cacttccgcg aaacggacaa agaaatcaac gaatttacgg ttggtccagc
    36121 taccgtattg ctgcaccaga tgcagcggca tttcaaagtg ggagagggtt atcaccggtt
    36181 cgatgttgta tttcagcagt tcatcgaaca gagagtcgta aaacttcagc ccttcttcgt
    36241 ttggctgagc ttcatcacct tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac
    36301 atttgaagcc catttcggca aatagcttga tgtcttcctt gtagtgacca taaaaatcaa
    36361 cggcttcatg gtttggatag tattttcctg gcaagacttc tttggtgatt tcgcgcggca
    36421 cgccgtgtgc gccacaggtc agaacgtcac aaatgctcgg tcctttgccg cctttgttcc
    36481 agccgccttc gacctgatga gcggcaacgg cgccacccca taagaaatct ttcggtaagg
    36541 tgagtttttt cactatcata ggctccagat cagtcaattc ttacaggaaa tagtaccatg
    36601 cagccaaacg acatcacttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa
    36661 ccatcaccat ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg
    36721 gacgttttga agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa
    36781 cgctggatac actgacggaa aaacatgcag agcaggaaaa tatgaccctg cctgaactga
    36841 aaaaggtcat tgctgacatc tatcccggtc agacacaatt ttatatgatt gaatttaaat
    36901 gtctttaaat tgaactcacg cctgttagtt gaaatttcgc atttaatttc atgattttta
    36961 ggagattagt ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc
    37021 agttacgctg gagaggtata catgtgcgtg tacgtttctc tggagtaagt tatggttcag
    37081 aagcccctca ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac
    37141 ggcattgggc tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat
    37201 ctcaatgcca gcgccacagc gataaccagt tacagcctct atggcggcag tatgatcctg
    37261 ctgttcctcg cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg
    37321 aagaaatttg accattgcgc tatttacctg ttgattgccg gaacctacac gccgtttttg
    37381 ctggtggggc tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctagca
    37441 ttgctgggga ttctgttcaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg
    37501 acttatctgg cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc
    37561 gcggcgggca gcgttacctt actggcggta ggcggcgtgg tttattcgct cggggtgatt
    37621 ttctacgtct gcaaacgcat tccatacaac catgccatct ggcacggctt cgtgctcggc
    37681 ggtagtgtgt gccactttct ggcgatctat ttgtatattg ggcaggcgta atgggtatgc
    37741 ctttgtaggc ctgataagac gcgtcagcgt cgcatcaggc atcggagcgc aaccgccgga
    37801 tgcggcgtga acgccttatc cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa
    37861 atcccagaca tgataagacg cgtcagcgtc gcatcaggcg ataaatgcct gatgcgatac
    37921 agagatttac tcttcgagcg aatacggcag cggctcgata tgcagtgtat tcgcatcgtc
    37981 gcgtacgcgg aagatgctgt ccggttccat atcgttattc atgacgacct gtaccacgac
    38041 ctgaccatct tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc
    38101 cattttcagc tctaagtctt caccagcttc cggcagtcgg ctggcactac ctgccagcaa
    38161 ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc gccaccatct cttgtccggt
    38221 ataacagcct ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat
    38281 aaactgcccg ctgttggcgg catcaatcac cgggaaacca gcttcaatgt ttaatgccag
    38341 ccactgttgg ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc
    38401 agtagcttca tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt
    38461 ggtcgcgcct tctttgacta cctgtttttc tttcgaaggc agttcgctaa agagatttgc
    38521 cagcgcggcg cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctggcgc
    38581 gatggtcact ttagagaata ccgcatattt tttcagttca gtcagctgcg gttcacgcac
    38641 gctgcgccgt tcaatccatg caaagccatc gccgtcgcgg aacagacgta aattgctcca
    38701 cattttacct ttggcgtcgc aatgggcggc gagcaggtgc tgatcttctg ccatctggct
    38761 gacatctgct gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc
    38821 aagcgcccag tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg
    38881 acgtggagga aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa
    38941 tgactaatgg taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct
    39001 tctgcgaggc gactgccaaa aaaatgaagc ccttgaattg ttgtgaatta atttcgcgat
    39061 cgcgatggtg tttactgata ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg
    39121 gtactggatt ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgaattcatt
    39181 gggcatgccg ccgtggtatg cgcgaactcg atatttcaat catgtcgttt ttcgaacatg
    39241 agtacgacag cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg
    39301 atccggacct gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa
    39361 taatggtccg actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg
    39421 cgcgtctcct ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt
    39481 attttactca tgccctggcc gctcagttac accccgttat ggatggtgtt actttcgctg
    39541 gtggtatttg attgcgttcg cagccagcgg tgtattaatg ctcgccaggg ggaaattcgc
    39601 ttgttgatgg acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg
    39661 tggatgatta agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat
    39721 ttatggctgg cagccgacag catggacgaa gccgaatggc gggatttacg gcggattttg
    39781 ttgcaacaag agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg
    39841 agatcaggcg taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg
    39901 ctcgtcgcta aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc
    39961 gtcagcaatc accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac
    40021 gaacttcacg cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca
    40081 ctcgccgtat cccagttgat cgccaagccc atacagcgca ataattttac cttcaaggtt
    40141 caggtcgtcg agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca
    40201 ggtcgggata cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt
    40261 gaggttatgt aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat
    40321 ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg
    40381 attctgctgt ggcttttgca gctagtgtat cagaatcgct ttagtcacgg cacaataagg
    40441 cataatggat caaatgagtg accataaggg gcgcaagtga aacaggagct ggcacgcatc
    40501 gagcagtttc ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct
    40561 taccgtcgcg atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg
    40621 acggcgcaaa gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa
    40681 gccaccagct cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc
    40741 gaaaagtttc gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt
    40801 ttgccaaaag atttaagcga agcgcaggtc gaacgtttat tacaggcgcc attaattgat
    40861 cagccactgg agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt
    40921 gtctctgaac tggtcgggct gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg
    40981 gtcattggta aaggcaacaa agagcgcctg gtgccgttag gggaagaggc ggtttactgg
    41041 ctggaaacct atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg
    41101 ttgtttccca gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa
    41161 cattatgccg tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac
    41221 gcttttgcca ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg
    41281 ggccacagcg atctttccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg
    41341 caacttcatc aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg
    41401 aagaaaggtt ttatgttgtt tactttgtta gcggcgtttt caggctttgc tcaggctgat
    41461 gacgcggcaa ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc
    41521 gcgcccgtag ctggcatgaa gacagttctg actaacagcg gtgtgttgta catcaccgat
    41581 gatggtaaac atatcattca ggggccaatg tatgacgtta gtggcacggc tccggtcaat
    41641 gtcaccaata agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat
    41701 aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc
    41761 cacaaactgc atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt
    41821 gctttcccgc gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt
    41881 gcgaaagata aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc
    41941 agctgcgacg tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt
    42001 actccggcag ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagac
    42061 atgaaagaat tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac
    42121 aacagataca acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc
    42181 ctcccttgct gcgccgttta tacgccagcc ggggagtacg cagtgcgcaa gaactggaac
    42241 gcagtgttaa aggtatgttg ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga
    42301 tcctttacaa cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg
    42361 acggcgcgac cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata
    42421 tcgactacct ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg
    42481 atcaggccca tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc
    42541 atgcgggagt tgagcacgct cgctcgttgg gcatcccggt tattgttacc gatcaccatt
    42601 tgccgggcga cacattaccc gcagcggaag cgatcattaa ccctaacttg cgcgactgta
    42661 atttcccgtc gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc
    42721 gcaccttttt gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt cctaacctgg
    42781 cagaactgct ggatctggta gcactgggga cagtagcgga cgtcgtgccg ctggacgcta
    42841 ataatcgcat tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccag
    42901 ggattaaagc gctgctggaa gtggcaaatc gcgatgcaca aaaactagcc gccagcgatt
    42961 taggttttgc gctgggaccg cgtctcaatg ctgccgggcg actggatgat atgtccgttg
    43021 gtgtggcgct gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg
    43081 atgcgctaaa ccagacgcga aaagagatcg aacaaggaat gcaggttgaa gccctgaccc
    43141 tgtgcgagaa actggagcgc agccgtgaca cgctacccgg cgggctggca atgtatcacc
    43201 ccgaatggca tcagggcgtt gtcggtattc tggcttcgcg tatcaaagag cgttttcacc
    43261 gtccggttat cgcctttgcg ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca
    43321 ttcaggggct gcatatgcgt gatgcgctgg agcgattaga cacactctac cccggcatga
    43381 tgctcaagtt tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg
    43441 aactctttca acaacggttt ggcgaactgg ttactgagtg gctggcccct tcgctattgc
    43501 aaggcgaagt ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc
    43561 agctgctgcg cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc
    43621 atttccgtct gctgcaacag cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac
    43681 cggtcggcgg cggtccactg ctggatggta ttgcttttaa tgtcgatacc gctctctggc
    43741 cggataacgg cgtgcgcgaa gtgcaactgg cttataagct cgatatcaac gagtttcgcg
    43801 gcaaccgcag cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct
    43861 ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca
    43921 ataaaagaaa tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca
    43981 cggaacgctc cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg
    44041 gaagaagtaa acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag
    44101 gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa
    44161 caggggctgg aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa
    44221 acctttaacg aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag
    44281 ttccgccgta tgttctctgg cgaatatgac agcgccgact gttacctcga tattcaggcg
    44341 gggtctggcg gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc
    44401 tgggcagaat cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg
    44461 ggtattaaat ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca
    44521 gaaactggcg ttcaccgcct ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac
    44581 acgtcgttca gctctgcgtt tgtttatccg gaagttgatg atgatattga tatcgaaatc
    44641 aacccggcgg atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt
    44701 aaccgtaccg aatctgcggt gcgtattacc cacatcccga ccgggatcgt gacccagtgc
    44761 cagaacgacc gttcccagca caagaacaaa gatcaggcca tgaagcagat gaaagcgaag
    44821 ctttatgaac tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa
    44881 tctgacatcg gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa
    44941 gatctgcgca ccggggtaga aacccgcaac acgcaggccg tgctggacgg cagcctggat
    45001 caatttatcg aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac
    45061 gcacagggcg ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag
    45121 ctggcgaacc tgcgtgagca gggaattgcc ttcccgaacg atttccgtcg cgatcatacc
    45181 tctgaccaat tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac
    45241 atcgaagtcg ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc
    45301 gttaccctgc aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg
    45361 gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa
    45421 ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg
    45481 ctgaccaaag cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg
    45541 cgctatcgtc agcgttatct ggatctcatc tctaacgatg aatcccgtaa cacctttaaa
    45601 gtacgctcgc agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa
    45661 gttgaaacgc cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc
    45721 caccataacg cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag
    45781 cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa
    45841 ggtatttccg tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca
    45901 gattacaaag atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt
    45961 ctcggtaaga cggaagtgac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa
    46021 aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg
    46081 gataacttcg actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc
    46141 tggggtctgg gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt
    46201 cagccgacct tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac
    46261 attaacccgg aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac
    46321 ggctttagcg agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc
    46381 gcgaaagacg caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg
    46441 gaacatggct taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg
    46501 ttcaccaaca gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa
    46561 taagcattac gttatgctca caaccccggc aaatgtcggg gttttttatt taagctgggt
    46621 aaatgcagat aatcgtattc tggcttcgcg atttgtcgcc tgcatcacca tccacggact
    46681 gaacgcccac ggcgtggcat caataccgcg taatacatca gctaaatcac accattgata
    46741 atccatcact tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg
    46801 acacacttca ttttccacaa tgcctctcgg atcggtggcg cggtagcgaa agtcaggata
    46861 gatagattca ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcaccgc
    46921 gtcttcgctg ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg
    46981 ccacgctttt ttgctcagtg cgcggcgggt aactaataat tgccccttgg cattaaacag
    47041 ccaactggag aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat acttttccag
    47101 cgtacccgtg ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc
    47161 tcacatgtaa ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa
    47221 gagagcgttt atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa
    47281 aaaagtagca tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg
    47341 acaggaatag agagagtaaa aagcaacaac agactgaata tagcgcgcat taaaaaattc
    47401 atattattct ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt
    47461 cctgacttct aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctgata
    47521 gccattaaag aagacgttca gcacaacggc gctaagggtt gcgagcataa tgccgctatg
    47581 tagcagcggt tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt
    47641 catgccaacg ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt
    47701 acagcgcgac agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ctagaccagc
    47761 gccgcccagc acaaattgcg gaatggaggc taccagcacc gccatttttg gcaccatgcc
    47821 aaacaggatt aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcatcac
    47881 gctaaccagg ccaacgtttt gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc
    47941 tatcattgtc cctacgccat cgacacgcag cccgcaaata atatcgtgtg aagagagttt
    48001 acgaccgact atttcaccca gtgccaggaa catccccatc gactcgataa acacgatgat
    48061 taacacggca gtcatggtca gaatggaaac gggatcgaaa atcggcatac caaacgacat
    48121 cggggtgaca atcgcaaacc acgaggcatc atgtagcccg gataaattga cttcattcat
    48181 catccacgaa agtaaaaagc caaatacaat ccccagtaat acggcgacgt tggacataaa
    48241 tcctttcgca tagcgagtaa tgagcaagat aaaaattaag acggcaaagg agatacctaa
    48301 ataaacggga ttaccatatt gcggattccc tttacctcca gcggcccagt caatacccac
    48361 ctgaatgatg ctaagcccaa tagaagtaat aaccacaccg gtaaccagtg gcgggaataa
    48421 aggcatcaag cgaccaataa gtggcgctaa taatgtggtg ataaaacccg cggcgatagt
    48481 ggcaccaaat atccccagca ggccgatatc cgggttcatc ccaatggcta tcattggtgt
    48541 tacagcagca aaggtcaccg acataatcac cggcaggcgg atccccataa agcggccgat
    48601 accgatacat tgcaataatg tgacgatccc gcagcaaaag agatccgagc taatgagcat
    48661 cgcaatagca tcttttgaga ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc
    48721 acctgcgtac atgacaagga cgtgttgcag accgagtatg atcagctttc ctggtgataa
    48781 tatgcggtca acctcatcag ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc
    48841 tatggcgctc atcagtgttt ctcctttcgc ccgccagaat aaaaagcaac cgtttacgtt
    48901 aatgaaactg tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag
    48961 agacaggaat tgcggaggca atcacataat cgtgcttaaa ccacgaaaat gacaagagaa
    49021 actgacaa
//
LOCUS       DAJTYL010000013        48296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYL010000013 DAJTYL010000000
VERSION     DAJTYL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.013"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..497)
                     /locus_tag="OO572_000777"
     CDS             complement(<1..497)
                     /locus_tag="OO572_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6659881.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYA"
     gene            865..1995
                     /gene="fruB"
                     /locus_tag="OO572_000778"
     CDS             865..1995
                     /gene="fruB"
                     /locus_tag="OO572_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6659882.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            1995..2933
                     /gene="fruK"
                     /locus_tag="OO572_000779"
     CDS             1995..2933
                     /gene="fruK"
                     /locus_tag="OO572_000779"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6659883.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            2950..4641
                     /gene="fruA"
                     /locus_tag="OO572_000780"
     CDS             2950..4641
                     /gene="fruA"
                     /locus_tag="OO572_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6659884.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5065..6006
                     /locus_tag="OO572_000781"
     CDS             5065..6006
                     /locus_tag="OO572_000781"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6659885.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            5994..6932
                     /gene="psuG"
                     /locus_tag="OO572_000782"
     CDS             5994..6932
                     /gene="psuG"
                     /locus_tag="OO572_000782"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6659886.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7026..8276
                     /locus_tag="OO572_000783"
     CDS             7026..8276
                     /locus_tag="OO572_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6659887.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8347..9041)
                     /gene="yeiL"
                     /locus_tag="OO572_000784"
                     /pseudo
     CDS             complement(8347..9041)
                     /gene="yeiL"
                     /locus_tag="OO572_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9172..10113
                     /gene="rihB"
                     /locus_tag="OO572_000785"
     CDS             9172..10113
                     /gene="rihB"
                     /locus_tag="OO572_000785"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6659888.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10213..11463
                     /locus_tag="OO572_000786"
     CDS             10213..11463
                     /locus_tag="OO572_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6659889.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11519..12607)
                     /locus_tag="OO572_000787"
     CDS             complement(11519..12607)
                     /locus_tag="OO572_000787"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6659890.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(12610..13467)
                     /gene="nfo"
                     /locus_tag="OO572_000788"
     CDS             complement(12610..13467)
                     /gene="nfo"
                     /locus_tag="OO572_000788"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6659891.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13541..14590)
                     /locus_tag="OO572_000789"
     CDS             complement(13541..14590)
                     /locus_tag="OO572_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6659892.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14689..15570
                     /locus_tag="OO572_000790"
     CDS             14689..15570
                     /locus_tag="OO572_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6659893.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15776..17245
                     /gene="lysP"
                     /locus_tag="OO572_000791"
     CDS             15776..17245
                     /gene="lysP"
                     /locus_tag="OO572_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6659894.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17537..19528
                     /gene="cirA"
                     /locus_tag="OO572_000792"
                     /pseudo
     CDS             17537..19528
                     /gene="cirA"
                     /locus_tag="OO572_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(19560..20395)
                     /gene="yeiG"
                     /locus_tag="OO572_000793"
                     /pseudo
     CDS             complement(19560..20395)
                     /gene="yeiG"
                     /locus_tag="OO572_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            20653..21321
                     /gene="folE"
                     /locus_tag="OO572_000794"
     CDS             20653..21321
                     /gene="folE"
                     /locus_tag="OO572_000794"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6659895.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21338..22414
                     /locus_tag="OO572_000795"
     CDS             21338..22414
                     /locus_tag="OO572_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6659896.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            22556..23596
                     /gene="galS"
                     /locus_tag="OO572_000796"
     CDS             22556..23596
                     /gene="galS"
                     /locus_tag="OO572_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6659897.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            23876..24874
                     /gene="mglB"
                     /locus_tag="OO572_000797"
     CDS             23876..24874
                     /gene="mglB"
                     /locus_tag="OO572_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6659898.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            24935..26455
                     /gene="mglA"
                     /locus_tag="OO572_000798"
     CDS             24935..26455
                     /gene="mglA"
                     /locus_tag="OO572_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6659899.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            26471..27481
                     /gene="mglC"
                     /locus_tag="OO572_000799"
     CDS             26471..27481
                     /gene="mglC"
                     /locus_tag="OO572_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6659900.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(27517..28730)
                     /gene="preA"
                     /locus_tag="OO572_000800"
                     /pseudo
     CDS             complement(27517..28730)
                     /gene="preA"
                     /locus_tag="OO572_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(28724..29962)
                     /gene="preT"
                     /locus_tag="OO572_000801"
     CDS             complement(28724..29962)
                     /gene="preT"
                     /locus_tag="OO572_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6659901.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30156..30395)
                     /locus_tag="OO572_000802"
     CDS             complement(30156..30395)
                     /locus_tag="OO572_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6659902.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(30398..31117)
                     /gene="sanA"
                     /locus_tag="OO572_000803"
     CDS             complement(30398..31117)
                     /gene="sanA"
                     /locus_tag="OO572_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6659903.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31267..32151)
                     /gene="cdd"
                     /locus_tag="OO572_000804"
     CDS             complement(31267..32151)
                     /gene="cdd"
                     /locus_tag="OO572_000804"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6659904.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32281..32976)
                     /locus_tag="OO572_000805"
     CDS             complement(32281..32976)
                     /locus_tag="OO572_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6659905.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(32973..33371)
                     /locus_tag="OO572_000806"
     CDS             complement(32973..33371)
                     /locus_tag="OO572_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6659906.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            33610..34557
                     /gene="dusC"
                     /locus_tag="OO572_000807"
     CDS             33610..34557
                     /gene="dusC"
                     /locus_tag="OO572_000807"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6659907.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(34928..35011)
                     /gene="yohP"
                     /locus_tag="OO572_000808"
     CDS             complement(34928..35011)
                     /gene="yohP"
                     /locus_tag="OO572_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6659908.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35235..36671
                     /gene="mdtQ"
                     /locus_tag="OO572_000809"
                     /pseudo
     CDS             35235..36671
                     /gene="mdtQ"
                     /locus_tag="OO572_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            36724..37485
                     /locus_tag="OO572_000810"
                     /pseudo
     CDS             36724..37485
                     /locus_tag="OO572_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(37602..38180)
                     /locus_tag="OO572_000811"
     CDS             complement(37602..38180)
                     /locus_tag="OO572_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6659909.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            38350..38937
                     /locus_tag="OO572_000812"
     CDS             38350..38937
                     /locus_tag="OO572_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6659910.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39111..40043
                     /gene="pbpG"
                     /locus_tag="OO572_000813"
     CDS             39111..40043
                     /gene="pbpG"
                     /locus_tag="OO572_000813"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6659911.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40081..41796)
                     /gene="dld"
                     /locus_tag="OO572_000814"
     CDS             complement(40081..41796)
                     /gene="dld"
                     /locus_tag="OO572_000814"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6659912.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            41992..44289
                     /gene="bglX"
                     /locus_tag="OO572_000815"
     CDS             41992..44289
                     /gene="bglX"
                     /locus_tag="OO572_000815"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6659913.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            44541..45458
                     /gene="osmF"
                     /locus_tag="OO572_000816"
     CDS             44541..45458
                     /gene="osmF"
                     /locus_tag="OO572_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6659914.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            45465..46622
                     /gene="yehY"
                     /locus_tag="OO572_000817"
     CDS             45465..46622
                     /gene="yehY"
                     /locus_tag="OO572_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6659915.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            46615..47541
                     /gene="yehX"
                     /locus_tag="OO572_000818"
     CDS             46615..47541
                     /gene="yehX"
                     /locus_tag="OO572_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6659916.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            47546..48277
                     /gene="yehW"
                     /locus_tag="OO572_000819"
     CDS             47546..48277
                     /gene="yehW"
                     /locus_tag="OO572_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6659917.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
BASE COUNT        11631 a        11868 c        12995 g        11802 t
ORIGIN      
        1 aacgcataag ccagcggtgg gccaatgacc catgccagtg aaacctgagc gcgtaaaaaa
       61 gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat gttcacgggc aagggcaaac
      121 atttgcgggt tagcggtcga gccaaagctg ctaagaaaga cgccaacgaa tagcaaaaca
      181 aagtagttgc gattccaagc aaaaagggtg caggccagca cgcctaacag gcagcaaaag
      241 acaatcagcg atttgcgatc gccgcgctta tcagagcgcc cagcgagaaa ctgacttacc
      301 agaatcccaa tgacagcgct gccggtaaag aagaatccca ccatcgccgg acgggcatgt
      361 acttcatcgg taaggaaaat actgagtgtc ggggtttgca gagcacccgc aataccggtg
      421 agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa acgatttcgc gctggagact
      481 gcgggggagt tatgcatgct ggtcgatcac tggaagagga agacgcggag tttacgctgc
      541 ttgtcagaaa ataaacagac agcgaaatca aaagcgccac gaataatcaa aatgccattt
      601 cactgttgct gattggctgc tgacgctgct gaaatgaaat tgctgataac ggattttccc
      661 atcagcaatt aggaaaaatg acaaaaaatt gtgcagcaca tcaaactttt gctcataact
      721 ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct ttcttgaaac gtttcagcgc
      781 gatcttgtct ttaaccctaa gctaggttgg cgcttttttt ctcatagagg ctgaatcgtt
      841 tcaattcagc aagagaggag aactatgttc cagttatccg tacaggacat ccatccgggc
      901 gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg ctgcggcgct ggtgcaggcc
      961 ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc gcgaacagca aacctcaacg
     1021 ttcctcggca atggtattgc tattccacac ggcactaccg acacccgcga tcaggtgctg
     1081 aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca cctggggtga cggtcaggta
     1141 gcgtacgtgg caatcggtat tgctgccagc tcggataaac atctgggcct gctacgccag
     1201 ctgacccacg tactgagcga tgattccgtt gctgaacaac tgaagtcagc aacaacagca
     1261 gaagaacttc gcgcattact gatgggcgaa aagcagagtg agcagctgaa gctcgacaac
     1321 gaaatgctga cgctggatat cgtcgccagc gatctgctga ctcttcaggc gctgaacgct
     1381 gcgcgtctga aagaggcgag ggcagttgac gccactttcg tcaccaaagc catcaatgaa
     1441 caaccgctga atctcggaca gggtatctgg ctgagcgata gcgccgaagg caatctgcgt
     1501 agtgcgattg cggtaagccg tgcggcaaat gcttttgatg tggacggcga aacggcagcc
     1561 atgctggtga gtgtggcgat gaatgacgat cagcccatcg cggttcttaa gcgtctcgct
     1621 gatttgttgc tcgacaataa agctgaccgc ttgctgaaag cggatgcggc aacgttgctg
     1681 gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt taagcgcgga gtttgtggtg
     1741 cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc tggtcaatac cattaaacaa
     1801 tttaacagtg atattaccgt gacaaacctt gatggcaccg gcaaaccggc aaacggacgt
     1861 agtctgatga aagttgtggc acttggcgtt aagaaaggtc atcgcctacg ctttaccgcc
     1921 cagggtgcag atgctgaaca ggcgctgaaa gccattggcg acgctatcgc tgctggtctt
     1981 ggggagggcg cataatgagc agacgtgttg ctacgatcac ccttaatccg gcttatgacc
     2041 ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa cctggtgaaa accaccggtc
     2101 tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt aaaagacctg ggaattgatg
     2161 tcaccgttgg cggcttcctg ggtaaagaca atcaggatgg ttttcagcaa ctgttcagcg
     2221 agctgggcat tgccaaccgt ttccaggttg tacaggggcg cacccgaatt aacgttaagc
     2281 tgacggaaaa agacggcgaa gtgaccgact tcaacttctc gggtttcgaa gtcacccccg
     2341 ccgactggga acgctttgtg actgattctc tgagctggct cggtcagttc gatatggtct
     2401 gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc gttcaccgac tggatgactc
     2461 gcctgcgtag tcagtgtcct tgcattatct ttgatagtag ccgtgaagcg ttagtagcag
     2521 gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg cgagttggaa atctgggcag
     2581 gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc acatgcgcta cgtgaacaag
     2641 gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc gctttgggtt aatgcctccg
     2701 gcgaatggat cgctaaacca ccgtcagtcg atgtcgtaag caccgttggc gcaggggatt
     2761 ctatggttgg tggcctgatt tatggcttgc tgatgcgtga atccagtgaa cacacactgc
     2821 gtctggcgac agctgttgca gccctggcgg taagtcaaag caatgtgggt attaccgatc
     2881 gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca accttttaac tgacagcagg
     2941 agaggcataa tgaaaacgct gctgattatt gacgctaatc tcggtcaggc acgcgcctat
     3001 atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa aactggaaat catcgacaat
     3061 ccgaacgacg cagaaatggc gattgttctc ggtgattcca tcccgaatga cagcgcgctg
     3121 aacggtaaaa atgtctggct gggtgatatt tcccgggcag ttgcgcaccc tgagctgttc
     3181 ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc cggtcactgc gacagcaccg
     3241 gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg cttgcccgac tggcgtagca
     3301 cacaccttta tggcggctga agccattgaa accgaagcga aaaaacgtgg ctggtgggtg
     3361 aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa tcactcccga ggaagtagcc
     3421 gcagcggatc tggtgattgt ggcggcagat atcgaagtgg atctggcgaa atttgctggt
     3481 aaaccgatgt atcgtacctc taccggtctg gcgctgaaga aaacctcgca ggaactggat
     3541 aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca aagctcaaac ggcgaccact
     3601 gaaggtaaga aagagagtgc aggcgcttat cgtcacttgc taacgggcgt ctcttacatg
     3661 ctgccgatgg tcgttgctgg tggtctgtgt atcgcgcttt cttttgcttt tggtatcgaa
     3721 gcgtttaaag agccgggtac gttggcagcg gcgctgatgc agattggtgg tggttcagcc
     3781 tttgcgctga tggtgccggt actggcaggt tatattgcct tctccattgc cgatcgtccg
     3841 ggcctcacgc cgggtctgat tggcggtatg ctggcggtca gcaccggttc tggcttcatt
     3901 ggcggtatta ttgcgggctt cctggctggt tacattgcga agttaatcag tacgcaactg
     3961 aaactgccgc agagtatgga ggcgctgaaa ccgatcctga tcattccgct aatttccagt
     4021 ctggtggtcg gtctggcgat gatctacctg atcggtaaac cagttgctgg cattctcgaa
     4081 gggctgactc actggctgca gaccatgggg actgcgaatg cggttctgct gggggcgatc
     4141 ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa acaaagcagc gtacgcattc
     4201 ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg cggcgattat ggcggcaggt
     4261 atggtgccac cgctggcaat gggcctggca acaatggtgg cgcgtcgcaa attcgacaaa
     4321 gcgcagcagg aaggtggcaa agccgctctg gtattgggac tgtgcttcat ttcggaaggt
     4381 gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc cgtgctgtat cgtgggtggg
     4441 gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac tgatggcacc gcacggtggt
     4501 ctgtttgttc tgctgatccc tggcgctatt acgccggtat tgggttacct ggtagcaatt
     4561 attgccggta cgctggtggc gggtttggcc tatgccttcc tgaaacgtcc ggaagtggac
     4621 gcagtagcga aagcagcgta ataaaaggtg tgttacaggg cagaaatttc ctgccctgta
     4681 acatttgatg agcgcaaaga gaatgaccag cttgtgaatg taattttatg aatttaaatg
     4741 gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca ctaaggttat ttcctcaagg
     4801 gcagattatt ttctgccctt ttttagtgtt cctgttctgt gcatcccgtc acaaattcct
     4861 cgcttttgta attcaggcat ttaatcgcat tttgtgagcc tgctcaaatc tgcccatttt
     4921 gcctctctgg cggtaattta ctttacgtaa aacatgcata ctatgagcac atgtttaaat
     4981 atgaacacat gtttaaaccg tctttgcagt atctatattc ttgtggattg acagggttgg
     5041 taatttttta ccagaggacg tgctatgcgc gaaaaggatt atgtcgtaat tataggttcg
     5101 gcgaatattg atgtcgccgg atattcacat gaatcattaa attatgcgga ttcaaatcca
     5161 ggtaaaataa aatttacgcc tggtggagta gggcgcaata ttgcacaaaa cctggcgttg
     5221 ctgggtaaca aagcctggct actgagcgcc gtaggcagtg atttttatgg tcaatcgctg
     5281 ctaacgcaaa ccaatcaatc tggtgtttat gtcgataaat gcctgattgt gccgggagaa
     5341 aatacgtcga gttatttatc attactcgat aataccggtg aaatgctggt tgctataaat
     5401 gacatgaata ttagcaacgc tattacagct gaatatctcg cacagcaccg tgaatttatt
     5461 cagagggcaa aggtcattgt cgcggactgt aatatcagtg aagaggcact ggcatggatt
     5521 ctggataatg ccgccaacgt acccgtattt gtcgatccgg tttcagcgtg gaaatgtgtc
     5581 aaagtgcgcg accgtctaaa tcagaaccac actctgaagc caaaccgcct tgaagcggaa
     5641 accctgagtg ggattgcgct gtcagggcgt gacgatgtgg caaaagttgc tgcctggttc
     5701 catcaacatg gcctgaaccg actggtattg agcatgggcg gcgacggcgt ttattacagc
     5761 gatatcagcg gtgaaaatgg ctggtctgcg ccgatcaaaa ccaatgttat taatgttacc
     5821 ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt gggtagacgg aatgccgttt
     5881 gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg cgctctcctg tgaatacacc
     5941 aataaccccg atttatcgat tgccaacgtt atatcgttag tggagaacgc agaatgtctg
     6001 aattaaaaat ttcccctgaa ttattacaaa tttctccgga agtgcaggac gctttaaaaa
     6061 acaaaaaacc ggttgtggcg ctggaatcga ccattatttc tcacgggatg ccgttcccac
     6121 aaaatgccca gaccgcaatt gaagtagaag aaactattcg taaacagggc gcagtacctg
     6181 ccactatcgc cattattggc ggcgtgatga aagtgggttt aagcaaagaa aaaattgaat
     6241 tactgggtcg tgaagggcat aacgtgacca aagttagtcg tcgcgattta ccttttgttg
     6301 ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac gatgattatt gcggcgcttg
     6361 ccggaattaa agtatttgcc accgggggaa ttggtggtgt gcatcgcggg gcggaacata
     6421 ccttcgatat ttctgccgat ttgcaagaac tggcaaatac taatgtcacc gttgtttgtg
     6481 ccggggcgaa atctattctc gatttaggat taaccactga gtatttagaa accttcggtg
     6541 tgccgttaat tggctatcag actaaagcgc tgcctgcgtt tttctgccgt accagctcgt
     6601 ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc ccgtgcaatg gcggtgaaat
     6661 ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc gatcccggaa cagtttgcga
     6721 tgccggagga atctatcaat gcagccatag atcaagccgt cgccgaagcc gaagagcagg
     6781 gcgttattgg taaagaaagt acaccgttcc tgctggcgcg cgttgctgag ctgaccagcg
     6841 gtgacagcct gaaatccaat atccagctgg tgttcaacaa cgccattctg gcgagtgaaa
     6901 ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac gggcagaaag ccgtcgcccg
     6961 tactcatcaa gcataccaaa acctggcgct gacgccgggt ttactggcat gaagggaaaa
     7021 tcattatgga tataatgaga agtgttgtgg ggatggtggt gttactggca atagcatttc
     7081 tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt tggagccgca ctgctgctgc
     7141 aaatcgctat tggtggcatc atgctctact tcccaccggg aaaatgggca gtagaacagg
     7201 cggcattagg cgttcataaa gtgatgtctt acagtgatgc cggtagcgcc ttcatttttg
     7261 gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg tgcgggtttt atcttcgcct
     7321 ttcgcgtact tccggcgatt attttcgtta ctgcgctcat cagtctgctg tactacattg
     7381 gcgtgatggg gctgctgatt cgcatccttg gcagcatttt ccagaaagcc ctcaacatca
     7441 gcaaaatcga atcttttgtt gcggttacca ctattttcct cgggcaaaat gagatcccgg
     7501 cgatcgttaa accgtttatc gatcgcgtga atcgcaacga gttgtttacc gcaatttgta
     7561 gcgggatggc gtccattgct ggttcggtga tgattggtta tgccggaatg ggcgtaccaa
     7621 ttgactacct gttagcggca tcgctgatgg cgatccccgg cggtattttg tttgcacgta
     7681 ttcttagccc ggcaaccgag ccttcgcagg tcacatttga aaatctgtcg ttcagcgaaa
     7741 cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc gatgaccggg ctgaaaatcg
     7801 ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat tattgcgctg atcaacggca
     7861 ttatcggcgg aattggcggc tggtttggtt tcgccaatgc ctctctggaa agtatttttg
     7921 gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga ctggagtgat gccaaccttg
     7981 ctggtagcct gattgggcag aaactggcga ttaacgaatt cgtcgcttac ctgagtttct
     8041 ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac cgtggcgatt atatcctttg
     8101 cgctttgtgg ttttgctaac tttggttcta tcggtgttgt cgttggcgca ttttcggcta
     8161 tttcgccaaa acgtgctcca gaaatcgccc agcttggttt acgggcgctg gcagcagcaa
     8221 cgctttccaa cctgatgagt gcgactattg ccgggttctt tattggtctg gcgtaatgct
     8281 cgatttgtag accggataaa acgtattaag cgttgcatcc ggcaatcgcg ctattgcaaa
     8341 taatttttac tgcatcatcc cggagaattt attctccggg tccatctcca gcgccagtcc
     8401 tgacaactgc tttctgtttt taattagata tcctttcttt taattaataa accgtcgtga
     8461 atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct
     8521 tgcgtatgct tttcgtgata aagatcacct tcttgtgaga gtaatataaa tgctgccagg
     8581 cgattcacta acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta
     8641 tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa cagcggacgg
     8701 taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt
     8761 tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggagcggc aaagaaatcg
     8821 atcagcgaca cgcgaccatt ggcaagcgtg gcgtaaagcc tggcgcggcc tcgcgtcaga
     8881 taaaacagcc atgacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga
     8941 aacagccgcg tatcggctga aacatccgtt gaaaagcaat ccttaaacgc agattcactc
     9001 atcaattgct gcttaaggtc attattgtgg atttctttca cgatgtttat ccgctatgag
     9061 atttctcata tttagcatac gtacgctgtt gttaagattc aatcattggc ccggtcgaat
     9121 tttctttgct gtagaaaaaa ataacgcaac tggaaacaga ggaaataaac aatggaaaag
     9181 agaaaaatta ttctggattg tgagccgggt catgatgatg ctattgcaat gatgatggca
     9241 gcgaaacatc cggcaataga tttattaggc atcactattg tagcgggtaa tcagacgctt
     9301 gataaaacat taattaatgg cctgaatgtt tgccagaaac tggagattaa tgttccggtt
     9361 tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg ttgccgataa tattcacggt
     9421 gaaaccggac tggatggccc ggtattcgag ccgctgaccc gccaggcaga aagtacccat
     9481 gcggtgaaat atatcatcga taccctgatg gcaagcgatg gcgatatcac tctggtgccg
     9541 gttggtccgc tttcaaatat cgcggtggca atgcgtatgc aacccgcaat cctgcccaaa
     9601 atccgtgaaa ttgtgctaat gggcggcgct tacggtacag gcaacttcac cccatctgcc
     9661 gagttcaata tctttgccga cccggaagcc gcccgcgtgg tgttcacctc cggcgttcca
     9721 ttagtgatga tgggcctcga tcttaccaac cagaccgttt gcaccccgga tgtaattgct
     9781 cggatggaaa gggtaggcgg ccctgccgga gagctgttca gcgacatcat gaacttcact
     9841 ctcaaaacgc agttcgaata ctacggcctg gctggcggcc cggtgcatga cgccacctgc
     9901 attggttatc tgatcaaccc tgatggcatt aaaacccagg atatgtacgt cgaagtggac
     9961 gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg aactgggcgt tcttggcaag
    10021 cccgctaata ccaaagtcgg catcactatt gataccgact ggttctgggg cttagtcgaa
    10081 gagtgcgtgc gcggctacat caaaacccat taagtgttga taacaagccg ggacagtatc
    10141 gcaagatgca ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa atgacatgaa
    10201 gagataatca ctatggatgt catgagaagt gttctgggaa tggtggtact gctggcaatt
    10261 gcgtttttac tgtcagtaaa caagaagaag atcagcctgc gtaccgttgg cgcggcgtta
    10321 gtgttacagg tcgtgattgg cgggattatg ctttggttac cgccagggcg ttgggtcgca
    10381 gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca gcgacgcagg tagcgcattt
    10441 atcttcggtt ctctggtcgg accgaaaatg gataccttat ttgatggcgc aggatttatc
    10501 tttggtttca gggtattacc ggcaattatc ttcgtcactg cactggtgag tattctctac
    10561 tacatcggtg tgatggggat cttaattcgc attctcggcg gtatcttcca gaaagcttta
    10621 aatatcagca agatcgagtc attcgtcgcg gtcaccacca ttttcctcgg gcaaaacgaa
    10681 attccggcga tcgtcaaacc ctttatcgat cgtctgaatc gcaatgaatt atttacagcg
    10741 atttgtagtg gcatggcctc gattgctggt tcgacaatga ttggttacgc cgcactgggc
    10801 gtacctgtgg aatatttgct ggcggcatcg ttaatggcga tccctggcgg gatcttgttt
    10861 gcccgcctgt taagcccggc tacggaatct tcgcaggttt cttttaataa cctctctttc
    10921 accgaaacac cgccaaaaag cattattgaa gccgatgcga caggggcaat gaccgggctg
    10981 aaaatcgccg ccggtgtagc gacagttgtt atggcatttg tcgccatcat tgcgttaatt
    11041 aacggtatta tcggcggcgt tggcggctgg tttggttttg aacatgcctc gctggagtcc
    11101 attttaggtt acctgctggc cccacttgcg tgggtaatgg gtgttgactg gagtgatgca
    11161 aatcttgccg ggagtttgat tggacagaaa ctggcaataa atgaatttgt cgcttatctc
    11221 aatttctcac cctatctgca aacggctggc actctggatg ctaaaaccgt ggcgattatt
    11281 tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg gggtggtggt gggggcgttt
    11341 tctgcggttg cgccacaccg tgcgccggaa atcgcccagc ttggtttacg cgcgctggcg
    11401 gcggcgacac tttctaatct gatgagtgcc accattgccg ggttctttat tggtttagcg
    11461 tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg tcgaatccgg caacattgtt
    11521 acaccattgg cactaatgac agcgcgttat cggcagacag ggtagggttg tttaagctgc
    11581 cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca cggaaattgt
    11641 ttccctccag aaagctatat accagacccg ccataaaacc atcgtcagca ccaaaactgt
    11701 ctaccgtcgt atgcgctggc acagtcagca aaaattgttc accatccttt tcgctgcaat
    11761 aaactgactc atcgggcaaa taaacaaaca gttgctgaac accttgctga tgcaatgcat
    11821 tcactgcggc attacggtca gcatcgctgg tgatcgcctg tccccataaa atttccagct
    11881 ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca gtgtttgatt ttgcccgctt
    11941 tgaattctga aacggtatcg acaaacaccg ggatctcatc agcgagggta aagacccatt
    12001 ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac gcccgcatga cgaagtaaat
    12061 cgcgcgaccc gttcagtagt tgcggtgtca actgttccag cagatgggta tcgttaattg
    12121 ccagcacggt ttgatcgtct cgattggcaa ttgccagata cgtcgatgtg ctttgaccat
    12181 gcaaacgaac gcagccggag acattcacgc cggcacggcg cgtttcttcc aggagcattt
    12241 cgccataaaa gtcatcgcca atcactgaaa gcaaatggac gtcacggcct aacagcgcca
    12301 gattgtgggc gatattgcgt ccaacgccgc ctgccgaaca atgaattgta ccgggatgag
    12361 aagccgcttg cgggtaatgg atatccgcca tcccgcgaat atccatattg attgtcccca
    12421 ccactacgca gtattcctgc tcggtgagaa tgtaaccttt gtctttaatc cggcctttgc
    12481 gcattaaatc cataatatgc gccgcaacgc gcgaacgact gatttgcagc atgtccgcaa
    12541 tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc aaggatctcc ttttcccggt
    12601 tatttatctt caggctaccg ctttttcagt ttgttgcgct ttcagccagg cgatctcttc
    12661 tgcccagata tccgggttga tggtttcaag gatcagcgga atgccgtcga aacggtcgtt
    12721 ctgcataatc cagcggaacg catcatgacc gatattgcct tcaccgaggc tatgatggcg
    12781 gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg tgcatcccgc gcaaatactt
    12841 aaagccgaca atacgggcaa aatcagcgaa cgttttttcg cattcggctg gagtacgcaa
    12901 atcatacccg gcagcgaaag catggcaggt atcaatgcag acgccgacgc gggatttatc
    12961 ttccacgccg tcgataatcg ccgcgagatg ttcgaattta aaccctaagt tactgccctg
    13021 accggcggtg ttttcaatca ccgctgttac accttgagtt ttatccagcg caatgttgat
    13081 ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg
    13141 gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc tgcatttcat ctataaaggc
    13201 atcgcgcgat ttttccagag cttcagtgac cggatgtccg aggttgatca gataactgtc
    13261 gtggggaaga atttgcgccg atgtgtagtg atatttttca caggcggctt tgaattcatc
    13321 gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc
    13381 aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca tttgccagac cgccagcagc
    13441 actaacgtgc gctccaatgt atttcatgcg aggactcctg ttaaacccgc tggaggaaaa
    13501 cggtaatgat agcgggttaa caggagcaag aagtagtggt ttatgcgatg acgctttgaa
    13561 tcacatagtt aatcgcacca ccaccaacaa tcagccaggc aaacagtacc agtgccatca
    13621 gcagaggttt cgccccagct tttttcagcg cgctgacgtg agtggtcaga cccagcgccg
    13681 ccatcgccat tgccagcagg aaggtatcca gcgttaccag catgttcacc acgctctgcg
    13741 gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa caagatggca aaccacggaa
    13801 tagtgatttt gcttttctcg ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca
    13861 gcaggatgag gaacggagcc agcatcatca cgcgcagcat tttggaaata actgctgcgt
    13921 tttctgcatc cgggctgatg gcatgacctg ccgccaccac ctgcgccact tcgtgcacag
    13981 tagaaccaat gtagataccg aaagtttccg gactaaacca ttgagacatc agcggatata
    14041 tcgccgggta gaggaaaatc gcgacggtcc cgaagataac aacggttgca acagccacgg
    14101 ttactttact ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac
    14161 agatactgct accggcaccg atcaaccaac tggtgtgctt atccagacca aacactttct
    14221 gccccaggaa gcaagccagc aggaaggtac tggacagcgt cagcacgtca atgatgatcc
    14281 cactgatacc gacatcggca atttgcgaga acgtcagacg gaagccataa agaatgatac
    14341 ccagacgtaa taaatattgc ttggcaaaca gcacaccacc gtcacagctt ttccagatgt
    14401 gcggatagat ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg agggcactaa
    14461 acccggcacc cgcaaccgcg ggaatggaac caccccacag ggcgaccccg gtgataactg
    14521 cactcagggc taaccccgga ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga
    14581 tattcgtcat aaccctctcc tttaaccggg cataaggtta cgactaactg gtttaaaaat
    14641 aaaattgatt atatatttat aattaatctt tataagtggt aagcgactat gcacatcacc
    14701 ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg
    14761 gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgactgacct ggaagggcag
    14821 cttggcgttc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg
    14881 ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg ttgaaatcga acaactgttt
    14941 cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa ctacattctg
    15001 cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga acttagcgtt
    15061 gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat tggctttatt
    15121 gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg
    15181 gttttcgccg cgccgacttc gccgttggct cgtggtccgg tcaccttaga acagctggcc
    15241 gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggatattgt cgattatctg
    15301 ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc cgaggcaatc
    15361 aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt gattgaagat
    15421 caattgcagg caggcacatt aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg
    15481 ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg ctttctggac
    15541 tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac aagaacatgc tggtgctgtg
    15601 tcgatttcgt gacgcagcgc cttcagcatg cattcgccag aaaagagatt ggctgcttta
    15661 cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg ccggaaggat
    15721 tgccaatcgt ctgctacaat cgcgccatca tttttaagat ggatagcatt tttgtatggg
    15781 ttccgaaact aaaactacag aagcgccggg cttacgccgt gaattaaagg cgcgtcacct
    15841 gacgatgatt gccattggcg gttccatcgg tacaggtctt tttgttgcct ctggcgcaac
    15901 gatttctcag gcaggtccgg gcggggcatt gctctcgtat atgctgattg gcctgatggt
    15961 ttacttcctg atgaccagtc tcggtgaatt ggctgcatat atgccggttt ccggttcgtt
    16021 tgccacttac ggtcagaact atgttgaaga aggctttggc ttcgcgctgg gctggaacta
    16081 ctggtacaac tgggcggtga ctattgccgt tgacctggtt gcagcacagc tggtcatgag
    16141 ctggtggttc ccggatacac cgggctggat ctggagtgcg ttgttcctcg gcgttatctt
    16201 cctgttgaac tacatctctg ttcgcggctt tggtgaagcg gaatactggt tctcactgat
    16261 caaagtcacg acagttattg tctttatcat cgttggtgtg ctgatgatta tcggtatctt
    16321 caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc ggcgaagcgc cgtttgctgg
    16381 tggttttgcg gcgatgatcg gcgtagctat gattgtcggc ttctctttcc agggaaccga
    16441 gctgatcggt attgctgcag gcgagtccga agatccggcg aaaaacattc cacgcgcggt
    16501 acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga ttatcagcct
    16561 gattattccg tataccgatc cgagcctgct gcgtaacgat gttaaagaca tcagcgtcag
    16621 tccgttcacc ctggtgttcc agcacgcggg tctgctctct gcggcggcgg tgatgaacgc
    16681 agttattctg acggcggtgc tgtcagcagg taactccggt atgtatgcgt ctactcgtat
    16741 gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaat tgtcgcgtgg
    16801 tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt gccggtttgt gcttcctgac
    16861 ctccatgttt ggcaaccaga cggtatacct gtggctgctg aacacctccg ggatgacggg
    16921 ttttatcgcc tggctgggga ttgccattag ccactatcgc ttccgtcgcg gttacgtatt
    16981 gcagggacac gacattaacg atctgccgta ccgttcaggt ttcttcccac tggggccgat
    17041 cttcgcattc attctgtgtc tgattatcac tttgggccag aactacgaag cgttcctgaa
    17101 agataccatt gactggggcg gcgtagcggc aacgtatatt ggtatcccgc tgttcctgat
    17161 tatttggttc ggctacaagc tgattaaagg aactcacttc gtacgctaca gcgaaatgaa
    17221 gttcccgcag aacgataaga aataagtttc ctcccttcct tgctaagccc tctcaaccga
    17281 gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt taacatttgg
    17341 attgataatt gttatcgttt gcattatcgt tacgacgcaa tcaaaaaagg ctgacaaatc
    17401 agaggctgtt ccggctttct gggatgatca cctgcataca aataagtcca ccgcgatgct
    17461 gccgtacgca aggggacgta aagaagatgt gagcgatacc cattttattt tcgtagttac
    17521 ctcatggaga tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc
    17581 cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt
    17641 cactgcatct tccgtggaac aaaatcttaa agatgcaccc gccagtatca gcgtcattac
    17701 ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca aagaagtgcc
    17761 tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc gttagtattc gtggtctgga
    17821 cagcagctac accctgattc ttgtcgacgg taaacgcgtt aactcccgca atgccgtctt
    17881 ccgccacaat gatttcgatc tgaactggat cccggtcgat tccatcgaac gtattgaagt
    17941 ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat
    18001 catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt accgtcgata ccaccattca
    18061 ggaacatcgc gatcgcggtg atacctataa cggtcagttc tttaccagtg gaccattaat
    18121 tgatggtgtg ctgggaatga aagcttacgg cagcctggca aaacgtgaaa aggatgaccc
    18181 gcaaaactca acgaccaccg ataccggaga aacgccgcgt attgaaggat tctccagccg
    18241 cgacggcaat gtcgaatttg cctagacacc gaatcaaaat cacgatttta ctgccggata
    18301 cggtttcgac cgtcaggatc gtgattccga ttcgctggac aaaaaccgcc tggaacgcca
    18361 gaactactgc gtcagccata atgggcgttg ggattacggc accagcgaac tgaaatccta
    18421 cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc ccgataactt ccgaaagcaa
    18481 tacggtcgac ggcaaataca cgttgccgct gacggcgatt aatcagtttc tcacggttgg
    18541 cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg gaaccagctc
    18601 caaaacgtct gccagccagt acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc
    18661 gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa acctacggtg aacactggag
    18721 tccgcgtgcc tacatggttt ataacgccac cgacaccgta acggtgaaag ggggctgggc
    18781 gacggcattt aaagcacctt ctctgttgca acttagccct gactggacga gcaattcctg
    18841 ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg
    18901 ggagctgggg ctttactaca tgggtgaaga aggctggctg gaagggggtg aatccagcgt
    18961 tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc agccgtacgt ctgacgtcaa
    19021 cgctgcaccg ggctaccaaa actttgttgg ttttgagacg ggcgctaacg gacggcgcat
    19081 accggtattt agctactaca gcgttaacaa agctcgtatt cagggcgtgg aaaccgaact
    19141 gaaaattccg ttcaacgatg aatggaaact gtcgatcaac tacacctaca acgatggtcg
    19201 tgatgtcagc aacggcgaaa acaaaccgct atccgatctg ccgttccata ctgctaacgg
    19261 tacgctggac tggaaaccgc tggcgctgga agactggtca ttctatgttt ctggtcacta
    19321 taccgggcag aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt ataccatctg
    19381 gaataccggc gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag gcgtgctgaa
    19441 ccttggcgac aaggatctca gtcgtgacga ctacagctat aacgaagacg gacgtcgtta
    19501 ctttatggca gtggattatc gcttctgatg agaagatgcc cggcgaaccg ggcggacttt
    19561 cacttcagta aatactgcgc atggaagcgc aggtgatcct ctataaaaga ggcgatgaag
    19621 tagtaactgt gatcatatcc cggctgaata cgcagcgtca tcggccacgc tttctggcgc
    19681 gcggcttctg ccaataccgc aggttgcaac tggtcggcaa gaaactgatc attatcgccc
    19741 tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg catacatcag cgcgcaacta
    19801 tcccattcca gcatgcattt ttgtcctcac ctaaataggt gctaaacgct ttgattcccc
    19861 acgggacgct gcacggattc acaatgggcg caaaggccga aacgctggtg tatttacccg
    19921 gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc
    19981 agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg cgcagataat
    20041 catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa aagcctgcgc
    20101 cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg
    20161 gcatcaccag tacaatcccc agttccgccg ctacccgctg ggcacccgcc ttggtggtga
    20221 agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc ggggtgtgat
    20281 cacgaggggg ggggagaaag atactgaacg tcatcgggca gtttaaggta ctggagtcgt
    20341 gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg
    20401 gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg tgctgtgagt
    20461 aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa aggcagcggc
    20521 aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg cacaataatc
    20581 aagctgtaaa tcagcttaat aactttgccc ccacgcaggg tggaggcgtc acacctgcag
    20641 gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt
    20701 gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc
    20761 aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct
    20821 gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc
    20881 tccggtctgg attacgccaa tttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc
    20941 gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca ccattttgtt
    21001 accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat cggtctgtca
    21061 aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg
    21121 cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctcgatc
    21181 gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca
    21241 acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc
    21301 gctgtgcgcc atcacaactg attaaaaggc aggaaccatg gagcgcaacg tcacgctcga
    21361 ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg cctttgggct
    21421 accaaaggcg gcttatctca atcccgcctg gtacgatgct attacgccgc aggatgcatg
    21481 gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt ttgcgctgct
    21541 gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt
    21601 aacgctgtta gtcttgttgg gctttattca cggtttattg ttctgggacg gcgatattct
    21661 gctggcttac gggctggtgg ggcttggcgt tttgctgtta ttggggctga tttccgacag
    21721 ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa aatactggaa
    21781 gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca acagtttact
    21841 ggcactgggc gcacagtatg gctggcaact ggcagggatg atgctcattg gtgcggcgct
    21901 gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc gtactggttt
    21961 tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc aatggcagct
    22021 ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc
    22081 gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat tgagccgctt
    22141 taagctggtg cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact atctattgca
    22201 aacgctgatt tgtaccacgc ttttttacca cctcggtttg tttatgcatt ttgaccgcct
    22261 ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct ctgttatctg
    22321 gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc
    22381 tgccggaccg gcaatatcta aaacatcaag ataacgatct ggattacatt cgttaacaaa
    22441 acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat tcagtgctga
    22501 caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag tgaatatgat
    22561 caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct
    22621 caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga
    22681 gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta gcgacaccat
    22741 tggcgtggtg gtgatggacg tttctgatgc gtttttcggc gcgctggtaa aagcggtgga
    22801 tctggtcgcc cagcagcatc agaaatacgt gctaatcggc aatagctatc atgaagcgga
    22861 aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca
    22921 ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc ccggtatggt
    22981 gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg ataatctcag
    23041 cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta ttggttatct
    23101 ttcttccagc cacggcattg aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt
    23161 gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc cggacatgcc
    23221 gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta ccgctgtatt
    23281 tgcttataac gacaatatgg ccgctggcgc actgacagca ttaaaagata atggcattgc
    23341 gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc gttacaccga
    23401 cccgcaatta acgaccgtgc gttatcccat tgcttcaatg gctaaattag ccaccgaact
    23461 ggccttgcag ggggcagcag gcaatattga tcctcgtgcc agccactgtt ttatgccgac
    23521 gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg gcggcgatca ctaactcaac
    23581 aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct taacaatgtg
    23641 atagctatga ttgcaccgtt ttaatgttgt aacccgtatg taacagtgaa taatcacttt
    23701 caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt taccgggcat
    23761 ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt aactctactg
    23821 ccccgccgag catttatctc aagcactacc ctgcataaga aaaaccggag ataccatgaa
    23881 taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg ccgctgcaca
    23941 cgctgctgat actcgcattg gtgtaacaat ctataagtac gacgataact ttatgtctgt
    24001 agtgcgcaag gctattgagc aagatgcgaa agccgcgcca gatgttcagc tgctgatgaa
    24061 tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc tggcgaaagg
    24121 ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg tgattgagaa
    24181 agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc gtaaggcgct
    24241 ggatagctac gacaaagcct actacgttgg cactgactcc aaagagtccg gcattattca
    24301 gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga acaaagacgg
    24361 tcagattcag ttcgtactgc tgaaaggtga accgggccat ccggatgcag aagcacgtac
    24421 cacttacgtg attaaagaat tgaacgataa aggcatcaaa actgaacagt tacagttaga
    24481 taccgcaatg tgggacaccg ctcaggcgaa agataagatg gacgcctggc tgtctggccc
    24541 gaacgccaac aaaatcgaag tggttatcgc caacaacgat gcgatggcaa tgggcgcggt
    24601 agaagcactg aaagcacaca acaagtccag cattccggtg tttggcgtcg atgcgctgcc
    24661 agaagcgctg gcgctggtga aatccggtgc actggcgggc acagtactga acgatgctaa
    24721 caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca aaggtgcggc
    24781 tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg ttggcgtaga
    24841 taaagacaac ttggctgaat tcagcaagaa ataagactga ttgggcgcag tcgtttactg
    24901 cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga ctccgtcttc
    24961 cggggaatac ttgttggaaa tgagcggtat caacaagtcc tttcctggtg ttaaggcact
    25021 tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg gggaaaacgg
    25081 tgcaggaaaa tcgacattat taaaatgcct gtttggtatt tatcaaaaag actccggcac
    25141 cattttattc cagggtaaag agatcgattt ccattccgcc aaagaggcac tggaaaatgg
    25201 tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga tggataacat
    25261 gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa tgtaccgcga
    25321 aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc gcgtcggcac
    25381 attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct ataacgcgaa
    25441 aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca atcatctgtt
    25501 cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct cgcataaaat
    25561 ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc agtggatcgc
    25621 caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg ttgggcgttc
    25681 tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc tcgaggtgcg
    25741 taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc tgcataaagg
    25801 ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg ttgagacgtt
    25861 atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac agatcaataa
    25921 ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg agcgccgctc
    25981 aacgggaatt tatgcctatc tggatattgg ttttaactcg ttaatttcca atattcgcaa
    26041 ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata cccagtgggt
    26101 gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt cgctctccgg
    26161 tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag aaatattaat
    26221 gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt atcagttaat
    26281 tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa tgcctgagtt
    26341 gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg gaattgtcga
    26401 tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc tttaagatta
    26461 ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga aagagggcgg
    26521 tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc caacattttt
    26581 aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta ttatcgcgct
    26641 cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc gtcaggtagg
    26701 gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca acaaagtgtt
    26761 cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg ccattggtgc
    26821 ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc cgttcattac
    26881 cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg actttgtcgg
    26941 ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg gctttgtcgc
    27001 gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg tggcgttcgt
    27061 ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg gcggtaaccc
    27121 ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac ctgctgatga tctacgcgtt
    27181 gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg gctctgccac
    27241 caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg taggcggtgt
    27301 atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa ttatttttac
    27361 cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca tactggcagt acatcatcaa
    27421 aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc gtaagaagtg
    27481 atgtgattgc ggatattgcc ggatgcgtct ctggttttac aacgttaccg ggtgttcttt
    27541 ctcgcctttc ttaaacttca cttccccgag ctcaatacaa cccaccgggc agacgtgacc
    27601 acaaagcaga caacccacac atttctcggt attacaatgc ggtgtgcggg ttttctcgct
    27661 ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc cacagccaac
    27721 acatttgtca agattgatac ggggatagac aatataactg cggtctaaat cttccgccgg
    27781 gacaatattg ttattcgcca gacctaccat ctcctgcaac gaatcaaatc cctgatcggc
    27841 gagataatgc gataacccgc tcgccatatc ttctacgatg cgatagccgt actgcataat
    27901 gccggtggtg acctgtaacg ttgctgcgcc gagcaataaa aactccgccg catcttccca
    27961 ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc agttctggat gggtgcgcat
    28021 ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc cggaaatact
    28081 cgatttaccg ttaacgatcg gcataccgat tttctgatta agatcgatat tggtgatgga
    28141 tttaacggtg ttaattgccg caatgccatc tgcgccgccg cgcttcgccg ccagcgccac
    28201 ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg tggaaccccg
    28261 tttcactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat cgctacccat
    28321 cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg cgccagctgc
    28381 tcccattgct gctcattttc ccccatgatc gaagcgatca atactttgtc cgggtaatct
    28441 tccttcagcc gacgcagggc ggtcagattc tcttccaacg gatgttcagc aatctgctcc
    28501 atatttttga agccgataaa accggtatct tctttcacca gatgatcaaa acgcggcgag
    28561 acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca caccgcccca acctgtgtcg
    28621 taggctttgg cacacatctc atagcagttg cctaccggcg aagaagagag gcagaacggg
    28681 ttgggaaagt tcacgccgca aaaagtaatc gaaagatctt tcgttaacat gagcaagctc
    28741 cctctaaata gtgatgaatc gcctcggcgg cttctttccc ggttttcacg gcatagacca
    28801 cggttttgtc accctcaaca atatcgccag cagcaaagac ttgcggatcg cggatctgat
    28861 aattttgtgt tttgatggtg ttacgctgct gctctaactc cgcaaaggca tccagtctgg
    28921 catgctgacc gacggcgaga ataattttat ctgccgccat cgtcagttcg cctgataacc
    28981 gtacatgctt aaacgtgact ttattgcctt ccacggctac tggcgtgaat ccatcgatga
    29041 tcgaaacgcc cagctcccgg gcgctggcaa actctttttc gcttgccgga aactcatcta
    29101 actcttcacg tgctacgcaa gttaccgcct gacagccgag aactttcagc gtgctggcta
    29161 cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct ttgtggaatg ctgatatcgc
    29221 cttgcgcctg tcgtacacgt tgcaggaagt cgacggcaat ctcaacgtcg ctatgctcaa
    29281 acagcggtag cccggaaccg cttgataacc ccacggtgac cagtaccgcg cggttttccg
    29341 ctttcagttg ctccagggtg agtgttttac cgatttcgtt attgcactta atggtcacgc
    29401 ccattttttc aatacgggcg atctctgcat ccagcactga ttgcggtaac cggaattgcg
    29461 gaataccgtt acgcaaccag ccaccggggt gcgcttcttt ctcataaatc gtgacgtcat
    29521 aaccctggtt tgtcagtgtc acactggcct gcaatccggc aggacctgcg ccaataatcg
    29581 cgactttgcc gagcgtttta gtaccgggct gataaatttc catcccggtt tgttgttcaa
    29641 aatcagtaac aaaacgctgt aagcggccga tatcaatggg agcatcaaca ccggcacggg
    29701 tacaaccgct ttggcataat ttctccgtcg ggcacactct ggcacaaacg gcaccgaggg
    29761 cattattttc gcgaattgtc tcggcagcgc ctttaaaatt acgaaagtag attgagcgaa
    29821 taaatttccc cggatcggtc tgtgccgggc aagcctgact acagggagcg tcgtgacata
    29881 ataaacagcg agaggcttct ttaatcgcca gtaacggcgt aaaagccgga gtgagttcat
    29941 ccagataatt ttgttgcggc atgtgatctt ccttaatgct atgagtaatt atggcgaacg
    30001 ctaaataaat attgcgtgct gatattaagg ctatgcgcgt ggggaaattt aagctgtgaa
    30061 aggattcaca aatagctgcg gttcagaatg ttatatccgc agcttattga gtgaagtaat
    30121 aattattctc aataattaac agaaaagaga aaatattaac gatagaaaat aagataaaca
    30181 atcatgccga tagataaaat tccgaagcca atatatgcca ccatataagc gaagaagagt
    30241 ccgggatttt ttgctcgcca gacatacacc ggttccggtg catttttaac ccgtttatca
    30301 atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac aaaatatcgg gataaggaaa
    30361 aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta ctttccttgt ttcttttgta
    30421 attcaagtaa ctgttcgggt gtaacggcgg gatacccctg cgcatcttcc ggcacctggt
    30481 gcatagccgg aatagggacc agcggcccta aaaaacgcgg ttcacgttta aaaatataaa
    30541 ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat acgtactgac agcatatctt
    30601 tcggtgacgg tacggcataa cactgagcct gaatccccat atgcagcgca ataaataatg
    30661 ctcgctcaca gtggaaacgt tgggtgataa taatgaaatc attagtatcg aaaactttgc
    30721 gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta atcgagaaca atatctgatg
    30781 ggtcgacacc agcagcgatt aaatctttgc gcatggtcat cggctcgtta taactttgca
    30841 atgcgttatc gccgctcagt aatagataat ttaccttacc gctgttatag gcattaatcg
    30901 ctccttgaat gcggtagcga taatactgat taataacgcc ggtacgataa tattttgctg
    30961 tgccgagcac cacgccgacc tggcggtagg ggagatcctg caattcgtcg tagatataag
    31021 gcgcggtttt ccagctcatc cagcgatcga ggccgagcac agtcaacagc agcaagccga
    31081 tcaggactaa caggctgagg aacacgcgct ttaacatgca ggagactcga gctaaggaaa
    31141 atttccatca ggctacttta cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg
    31201 gaatttcgac accaggagag ggctggcaag ccggacaagg cgttcacgcc gcatccggca
    31261 ccaggcttaa gcgagaagca cgcggtcgat actgtgacag ccgagagctt tcagcgttgc
    31321 ggaggtggca tcccactgaa tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg
    31381 gatatccggg taatcataac ccttgagatt caacagaatt aacgccccct gcaacggcgg
    31441 cagagtcggg ttgaatgcgg cgttttcagc gtagctgccg ctgaaaatac gaccgtcttt
    31501 acattccagc gcgacaccgc ttggtgactt actgtaaggc atgtgcgaac ggtttgccgc
    31561 tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc
    31621 catcagcagc gttttaatct ccagatcttt cggcccaaag gcatccggca gatagtcacg
    31681 cagcgcgtgt gcctcgcggc ccggcaaatg aatacgcaga tccagaccgc tgttcagttc
    31741 attcataaac tgacggcagt gaccacaagg cgtatagtta acggtgatgg ctgcaagcgc
    31801 tttttcacca ctcaaccaag cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg
    31861 cattgttgca ccaataaatt ccatattggc accaaaatac caggttccgc tcacaccgcg
    31921 cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg
    31981 aagtagtgcg aatgccagcg cgtcttcgtc cagtcccgtt gcgctcttca gcgatgagac
    32041 ttgctccccg gtcaacaaag cggggaagta cttgtctgcc agaataggtt ccagtgcaga
    32101 ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc
    32161 tcataatcat gtaatgggat aacagtttac gaacccgtta tggctttata tgtgatctga
    32221 atctcattaa ttatgcaaca cgtgaaactt acatcaaaaa tgcgcgacgt atcgcaaatt
    32281 ttagcccatt actgccagaa taatcgggaa aaggaacggt gcgatcagcg aagtaattat
    32341 cccgcataac accagcgcta gcgaactaaa tgcaccttcc tgataatcca gctcggcgca
    32401 gcgcgccgta ccgagggcgt gcgaggcagt ccccattgcc agaccgcgcg cagctttggt
    32461 acgaatacgc atcgcattaa gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa
    32521 aatcacgcaa actgcgctga ttgccggaat accgccaata ctgccgccaa ccgccattgc
    32581 aataggcgtg gtgactgatt tcggcaggat tgacgcggcg atttccggtg aagcgcccat
    32641 caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg ctgccgataa aacagatggt
    32701 gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc tcatataaag gataggccag
    32761 cgcgacgacc gccggttgca gcagatcgtt tagcacttcg ctacctttaa agtaactgtc
    32821 gtaagagatg ccggtcagca ttaaaaaagg aatgatcacc accattgcta ccagcaacgg
    32881 gttaagcagg ggaaacttat accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa
    32941 cgtcagcggt aatgaccacc agatattcgc catcattctt ctgatccttt ctgacctacg
    33001 actttacgtt caccgtgtac cagttgtgaa ctccagctca ccaccagaaa aaccaccagc
    33061 gtactgacag cacaggaaac cactaccggg ccaaactgcg cgcggagcaa atcaaaatat
    33121 tgcatgacgc ctacgccaat cggcacaaac aatagcgcca tatagcgaat cagtacgtag
    33181 caccccggat tgacccattt cgccggaaga atttgcaagg ccagcaggac aaacaggatc
    33241 agcatcccga tgatgctgcc cggaatggtt accggtagca gggaagcaat gaaaatgcct
    33301 gcatacaggc aggcataaat caggacgaaa gcgcgtaaat attgccagat aatgttcagt
    33361 gtcttactca taatcaacac cccagatgag atggattcat catacaatta attcgtaaaa
    33421 cgtgctaccg atcacattaa gaaatcagcg cttatttttt cgctacccgg tattacgcaa
    33481 tgagcagcga actcaggcat acatcaagca tagagaggga ttgcagtttg cagcaatccc
    33541 tctttgcaga cctttaatcc gctgaccata ggcgctaaca tagcgcctca tttttttgcg
    33601 ggtgatgata tgcgtgtatt actggcaccg atggagggag tgcttgactc tctggtgcgt
    33661 gaattgctga ccgaagttaa cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg
    33721 gatcaactgc tgccggtaaa agtctttcat cgcatttgcc ctgagctaca aaacgccagc
    33781 cggacaccat ctggtacgct ggtgcgcgtg cagttgttag gtcagttccc acaatggctg
    33841 gcagagaacg ccgcccgtgc ggtggagtta ggtttctggg gcgtggatct caattgcggc
    33901 tgcccgtcga aaacggttaa cggtagcggc ggcggggcga cgttactcaa agatcctgaa
    33961 ctcatctacc agggggcaaa agcgatgcgt gaagctgtac cggcgcattt gcctgtcagc
    34021 gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt
    34081 caacaggctg gcgctacgga gctggtggtg catgggcgga cgaaagagca gggttaccgc
    34141 gcggagcata ttgactggca ggcgattggc gagattcgcc agcggctgaa tattccggtg
    34201 attgccaacg gtgaaatctg ggactggcag agcgcgcaac aatgcatggc gatcagcggc
    34261 tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc ccaacctgag ccgggtggta
    34321 aaatataacg aaccgcgaat gccgtggccg gaggtggttg ctttgctgca aaaatatacc
    34381 cgtctggaaa agcagggcga taccgggtta tatcacgttg cgcggattaa acagtggttg
    34441 agttatttgc gtaaagaata cgatgaagca acggaattat ttcagcatgt tcgggtgttg
    34501 aataactccc ctgatattgc aagggctatt caggcaattg atatcgagaa actctaaaaa
    34561 ccatagtgat gtttttgaca atgtgttagc tctttatcaa taggccgcac ggttgtttgg
    34621 tggcgtcaaa atcgccgagg cgttccctga aggccggggc agcccacatg gatgtgggct
    34681 gagggcgcgt tttacaggga tgttacctcg cgcccgaccc ggtagccgta agggataagc
    34741 cgagggcacc gcgcagcggc gattttgttc gccagagccc gggggggcag ggggcggcgg
    34801 cgattggtcg ccccctgcgc gctccttgcg ccagtgatta tatgaagcaa tgcacatgac
    34861 aggagcgcaa caggatgaga gactgaaata ttctcgttat tttcggatct gatgacatta
    34921 attaatttta aaatatcatc ttaaatacgc cagtcaccac cagtagccca atcaggaaaa
    34981 taatcaatac agcccagagt ataattttca ttggcgttcc tttatttgtg agtgctgaat
    35041 aagtatagga aaaaacgaag ccctttgccg ttatccataa acgattaaga taaatcggaa
    35101 gatatcattt acttagtcta tatttttctg ggtatgtcgt tttattgcta ctgatgtatt
    35161 gccacgcgaa actaaaaaat atatcgcaac acacggtaat ctccgcgcac cgtttcttac
    35221 ctgattattc ccccatgaat cgtgattcct tttatccagc catcgcctgt tttccgctgt
    35281 tactgatgct ggccgggtgt gcgcctatgc atgaaacccg ccaggcgtta agccagcaaa
    35341 cgcccgctgc acaagttgac accgcattac ccacggcgct gaaaaatggt tggccagaca
    35401 gccaatggtg gctggagtat cacgataatc agctcacttc attaattaac aatgcactac
    35461 agaacgcacc ggatatgcag gtggctgaac agcgcattta gctagctgaa gcccaggcga
    35521 aggcagttgc cactcaggat ggtccgcaga tcgacttttc ggcggatatg gagcggcaaa
    35581 aaatgtcggc agaagggcta atggggccgt ttgctctgaa cgatccggcc gcaggtacga
    35641 ccggtccgtg gtacaccaac ggaacttttg gcttaacggc gggctggcat ctcgatatct
    35701 ggggaaagaa tcgggcggag gttactgccc gcctgggtac ggttaaagca cgggcggcgg
    35761 aacgcgagca aacccgccaa ttgctggctg gcagcgtagc ccgcctgtac tgggagtggc
    35821 aaacccaggc ggcgttaaac acggtcttgc agcaaataga aaaagagcag aacaccatta
    35881 tcgcgaccga tcgccagcta tatcagaacg ggattacttc ttcagttgac ggtgtggaaa
    35941 ccgatattaa tgccagcaaa acccggcagc agctcaacga tgtcgcgggg aaaatgaaaa
    36001 ttattgaggc acggttaaat gcactcacaa atcaccagac taagtcattg aagcttaaac
    36061 cggtcgcgtt gccgaaagtg gcaagccagc ttcctgatga actggggtac tccttactgg
    36121 cccggcgggc agatttgcag gcggcgcact ggtacgttga gtcatcgtta agcactattg
    36181 atgcggcaaa agcggcattt tatcctgaca tcaacctgat ggccttcctg caacaggatg
    36241 cgttgcactt aagcgatctg ttccgtcatt ccgcgcagca aatgggcgtt acggcaggcc
    36301 tgacgctacc tattttcgat agtggtcgtc ttaacgccaa tctcgatatc gcaaaagccg
    36361 aaagcaactt gtctatcgcc agctacaaca aagcggtggt tgaagcagtg aatgacgtgg
    36421 cgcgggcagc cagtcaggtt cagacactgg cggagaaaaa ccagcatcag gcgcaaattg
    36481 agcgcgatgc cttgcgtgtg gtaggtcttg cgcaggcccg ctttaacgcg ggcatcattg
    36541 ctggttcccg cgtcagcgaa gccagaatcc cggcgctgcg tgagcgggcc aatggcctgt
    36601 tattgcaagg gcagtggctg gatgcctcca ttcgactcac cggtgcgctg ggcgggggtt
    36661 acaaacgctg atgatatgcg ccttctatac ttaacgttta ttcagcgtta agtggagaac
    36721 tcgatggcat aggtcgcgat aattaccgct tccgattcgg ggatcggcaa agagtgcgcg
    36781 ttattactgg cgcagcaggg gtttgatatt ggcattacct ggcactcaga tgaagaaggg
    36841 gcaaaagata ccgcgcgtga ggtggttacc cacggcgtac gtgcggagat cgtgcaactg
    36901 gatctcggca atctgccaga aggtgcgcag gcgctggaga cactcatcca gcgatttggg
    36961 cgtattgatg tgctggtgaa taatgcgggt gcaatgacca aagcgccgtt tcttgatatg
    37021 gcttttgatg agtggcgcaa gatttttacc gttgatgttg atggtgcatt tttatgctcg
    37081 caaattgcgg ctcgtcagat ggtgaaataa gggcagggcg gtcgcatcat caacattacg
    37141 tcggtacatg aacatacgcc gctgccggat gccagcgcct acacagccgc taaacatgcg
    37201 ctcggcgggt taaccaaaac gatggcgctg gagttggtca ggcataagat tttggtgaac
    37261 gcagtcgcgc ctggggcgat agccacgcca atgaacagca tggatgatag cgacgtgaag
    37321 cccgacgcgg agccttcgat tcctctacgg cgttttggcg caacgcatga gattgccagc
    37381 ctggtggtgt ggctttgttc ggagggcgca aattacacga cagggcagtc gttgatagtg
    37441 gatggcggct ttatgttggc gaatccgcag ttcaacccag aatagcgcag tgaggtgcgt
    37501 ggatatttca atgggttagg ccagataaga caccagcgtc gcatcaggca tggaacaccc
    37561 ggtgccggat gtggagtgaa tgcttatctg tggcaactgc tttacgcctg attatccggc
    37621 tttttcttcc cgcgccgttt cagccaccag cgcacgccca ccaccagaac cacgaccaga
    37681 atcaaccaga cccagtgctt caaatgctgg tcgagattgt gcaaccacgg cgcaatcacc
    37741 tgaccaccag cgtaaccaat agtggtaaaa atcaacgccc aggcaaatgc gccgagaata
    37801 ttcagcggca gaaagatttt cggcggcagc tggctggcac caatcagcgt cgggccaatc
    37861 acccgaaagc catacataaa gcgcgtacca atgacaaaca gatacggatg gcgttggata
    37921 agtttctgtg cccgctcaat tttatcctga tgtttcgaga aacggcgtaa cagcttgccg
    37981 ccaaaccgcc gcccgcacag atagagcacc tggtcgccaa tcatgccacc aagcgccaca
    38041 gaaagtacca ccagtgggaa ctttaatagc ccctgatgtg ccgcaacgcc tcccagcaaa
    38101 gtcacggttt caccttccgc caggctaccg ataaccagcg cggcataacc atattgtgag
    38161 atgagtgtat tgagatccat aggccagttt gtctcccttt ggttgcctgt aagcatacac
    38221 cctgtgaatc attcacgctg aaaaaggaca tacatcagac ggtgcaaatt acatggatga
    38281 acaaaaaata atcacaagct ggcttatact tgaactgtca ggttcatgaa ctcacaacaa
    38341 gggggcggta tgagccatgt ctggggactg ttttcccatc ccgatcgtga aatgcaggtg
    38401 attaatcgcg aaaacgaaac gatttctcac cattacaccc accacgtttt gctgatggcg
    38461 gcgattccgg tgatttgcgc cttcattggc actacacaga ttggctggaa ttttggcgat
    38521 ggcactatcc tgaagttatc ctggtttacc ggactggcgc tggctgtctt gttttacggc
    38581 gttatgctgg ctggggttgc ggtgatgggg cgggtcatct ggtggatggc gcgcaattac
    38641 ccgcagcgtc cgtctcttgc gcactgcatg gtcttcgcgg gctacgttgc aactccgctg
    38701 tttttaagcg gtctggtggc gctttatcca ctggtctggt tgtgtgcgct ggtcggcacg
    38761 gtggcgctgt tttacaccgg ctacctgctt taccttggta ttccgagctt cctgaatatc
    38821 aacaaagagg aaggccttag cttctccagt tcgacgctgg cgattggcgt gctggtactg
    38881 gaagtgctgc tcaccctaac cgttatacta tggggctacg gttatcgttt gttctagaaa
    38941 gttctttgac gttgcattgc tggcgtaaat gtcagcaatg cagcatttac tcacgattct
    39001 cctttgacga tcgcactttt tgctcgttat gatggcgtcg ctagcctcag taaatcctta
    39061 ctccggcggt gcgcaacccg tgcgcgtgaa ccactatctg aatgctcatc atgccgaaat
    39121 ttcgagtttc tttatttagc ttggccctga tgctggctgt gccttttgca ccgcaggccg
    39181 ttgcgaaaac ggcagccgct accaccgctt cacaaccgga aattgcctcc ggtagcgcga
    39241 tgattgttga tctgaatacc aacaaagtga tctattcgaa ccacccggat ctggtgcgtc
    39301 cgattgcgtc tatcagcaaa ttaatgaccg cgatggttgt gctggatgca cgactgccgc
    39361 tggatgaaaa actaaaagtg gatatcagcc agacgccgga gatgaaaggg gtctattcgc
    39421 gcgtacgact gaatagcgaa atcagccgta aagatatgct gttgctggcg ctgatgtctt
    39481 cagaaaaccg cgcggcggca agccttgcgc accattatcc cggtggttac aaagccttta
    39541 ttaaggcaat gaatgcgaaa gcgaaatcgc tcggaatgaa caacacgcgc tttgttgaac
    39601 ctaccggatt gtcggtgcat aacgtttcaa ctgcccgtga cttaaccaaa ctgctcattg
    39661 ccagcaaaca atatccgttg atcgggcagt taagtactac ccgggaagat atggcaacct
    39721 tctctaaccc gacgtatacg ctgccgttcc gcaatactaa tcatctggtg tatcgcgata
    39781 actggaatat tcagttaacc aaaaccggct ttaccaatgc ggcgggccat tgtctggtga
    39841 tgcgtacggt tatcaataat aaaccggtgg cgctggtagt gatggacgcg tttggcaaat
    39901 atacccattt tgccgatgcc agccgcctgc gtacctggat tgaaaccggt aaagtgatgc
    39961 ctgtgccggc agcagcgttg agctataaaa aacaaaaagc cgcccaaatg gcggcggcgg
    40021 ggcagacggc acagaacgat taagtgaatt cggatggcga tactctgcca tccgtaattt
    40081 ttactccact tcctgccagt ttttccgttt actggtttta ccgatccccg gattcatgct
    40141 attggtcgga tcattctcgc gatagaactt ctgcaacgtc tccggtgctt tatacaaatg
    40201 accgacgtta tgctcggcag ggtactgcgc gccgcgctgc tgtagcagtt ccagcatctg
    40261 ttcttttaac gcatgcacat ccacgccttt tttcactatg taatcctgat ggaagacata
    40321 gcacataaaa tggccgtaat agagcttgtg caccagctgg ctgtcgatct ccggcggtaa
    40381 atgctcatac cactcggtgt cgttacgccg cagagcgata tccagtgcca gaatgtcttc
    40441 gacttcatcg gaatgcaccg cctgataacg aattgctgcg cccgcagcgg cgaaacggtg
    40501 taaaaacgct ttgctacctt cctccggcgt acagacaaag aaatcgccct cggcctgttt
    40561 gaaataatcc accagccacg atttggcttc accgacgcca tcgcctgcca tttttaacag
    40621 cagatgatgc tcgtatttat cgcgccagtt tttcatgcgc ggcggtaaat ggctggggaa
    40681 caggtgaccg aatttttgca ttgcacggtc agtaaaatgc ggacggaaga atttcacttt
    40741 ctccagcatc gcatcggtgc gtcccttgag attaaagaag aacggcatct tgtcggtgcc
    40801 taacttatca atcatcagga aggtgtcttt gccgtatttt tccgcaatat cgtagatatc
    40861 ccggtgcata tattccccgg caaccggcag attttcgaag ttagccagaa tatgacggcg
    40921 gatttcggtc agcacttccg gctggttggt gccgatataa aacacctgct gatttttttc
    40981 cgcttcgaag gtatcaagac gtaccgcaaa gacggccagc ttcccggcgc aaccagaaga
    41041 ttcaaataac cgatccggat cggcgttata acgtgcgggc gtgtcggctt caatatcacg
    41101 aacgcggtgg acatagtcat aatcgtgggc gtgacgacca tcgtgacgca catcgtcatc
    41161 tttgatgcga tcgtcatcca gcttgctaag gatctgctcc ggcgtttcgc ccagatcaat
    41221 ccccagatgg ttcaccagcg tcagtttgcc atcttcatct atacgcgcga ataacgacat
    41281 ttcggtatac gccgggccac gttgcaccag cgagccgccg gagttgttac aaataccgcc
    41341 gatgaccgat gcgcctatac acgatgatcc aatcaccgaa tgcggttcgc gtcccagcgg
    41401 tttgagggct ttttccagcg aatagagcgt ggtgcccgga taggccagca cctgttcgcc
    41461 cttgccgaga acgtgcagct tgtcgagacg cagggtgctg atgatcacga tatcgcgatc
    41521 atagtcgtta ccgtttggcg tcgatccttc ggtcaggcct gtattggcag cctgcatcag
    41581 aataattttg tcggcggtga cgcaggcttt cagcacccgc cacaattcca gtagtgagcc
    41641 agggaaaacg acagccagcg cgtcgccctg accagaacgg aagcccttgc gatagcgggc
    41701 cgtttttgcg ggatcggtga gcaggtgtga atgaccgacc agacgggcaa gttcattcaa
    41761 aaaggcttta ttatcagttg ttgtcatgga agacattttc cactccttgt ggtggcgaaa
    41821 aaatatcaca aactagaata gcgccttgta tgacaattca gcgagtgaat attttgaaaa
    41881 ttcagacaat aaactgagca ttctttttct ctctccatca tgcttatggc aaactacggt
    41941 ttttgcacca ttttcgctgc cacgtcgggc aacaaaggaa gaaaaatcca tatgaaatgg
    42001 ctatgttcag taggaatcgc ggtgagtctg gtcctgcagc cagcactggc ggatgattta
    42061 ttcggtaacc atccattaac gcctgaagcg cgggatgcgt tcgtcaccga tctgctcaag
    42121 aaaatgacgg ttgatgagaa aattggtcag ctgcgcttaa tcagcgtagg cccggataat
    42181 ccgaaagagg cgatccgcga gatgatcaaa gacggtcagg ttggggcgat tttcaacacc
    42241 gtaacccgtc aggatatccg cgccatgcag gatcaggtga tggaattaag ccgcctgaaa
    42301 attcctcttt tctttgctta cgacgtgctg cacggtcagc gcacggtgtt cccgattagc
    42361 ctcggtctgg cctcgtcttt taacctcgat gcggtgaaaa cggtcgggcg tgtctctgct
    42421 tatgaagcgg cagatgatgg cctgaatatg acctgggcac cgatggtcga tgtctcgcgc
    42481 gatccgcgct ggggacgtgc ttccgaaggt tttggcgaag atacgtatct cacctcaata
    42541 atgggtaaaa ccatggtgga agcgatgcag ggtaaaagcc cggcagatcg ttactcggtg
    42601 atgaccagcg tcaaacactt tgccgcatac ggcgcggtag aaggcggtaa agagtacaac
    42661 accgtcgata tgagtccgca gcgcctgttt aatgattata tgccgccgta caaagcgggg
    42721 ctggacgcag gcagcggcgc ggtgatggtg gcgctgaact cgctgaacgg cacgccagcc
    42781 acctccgatt cctggctgct gaaagatgtt ctgcgcgacc agtggggctt taaaggcatc
    42841 accgtttccg atcacggtgc aatcaaagag ctgattaaac atggcacggc ggcagacccg
    42901 gaagatgcgg tgcgcgtggc gctgaaatcc ggaatcaaca tgagtatgag cgacgagtac
    42961 tactcgaagt atctgcctgg gttgattaaa tccggcaaag tgaccatggc agagctggac
    43021 gatgctgccc gccatgtact gaacgttaaa tatgatatgg ggttgtttaa cgacccatac
    43081 agccatttgg ggccgaaaga gtctgacccg gtggatacca atgccgaaag ccgcctgcac
    43141 cgtaaagaag cgcgtgaagt ggcgcgcgaa agcctggtgt tgctgaaaaa ccgtctcgaa
    43201 acgttaccgc tgaaaaaatc ggccaccatt gcggtggttg ggccactggc ggacagtaaa
    43261 cgtgacgtga tgggcagctg gtccgcagcc ggtgttgccg atcaatccgt gaccgtactg
    43321 accgggatta aaaattccgt cggtgaaaac ggtaaagtgc tgtatgccaa aggggcgaac
    43381 gttaccagtg acaaaggcat tatcgatttc ctgaatcagt atgaagaagc ggtcaaagtc
    43441 gatccgcgtt cgccgcaaga gatgattgat gaagcggtgc agacggcgaa acaatctgat
    43501 gtggtggtgg ctgtagtcgg tgaagcacag gggatggcgc acgaagcctc cagccggacc
    43561 gatatcacta ttccgcaagg ccaacgtgac ttgattgcgg cgctgaaagc caccggtaaa
    43621 ccgctggtgc tggtgctgat gaacgggcgt ccgctggcgc tggtgaaaga agatcagcag
    43681 gctgatgcga ttctggaaac ctggtttgcg gggactgaag gcggtaatgc aattgccgat
    43741 gtattgtttg gcgattacaa cccgtccggc aagctgccaa tttccttccc gcgttctgtc
    43801 gggcagatcc cggtgtacta cagccatctg aacaccggtc gtccgtataa tgccgacaag
    43861 ccgaacaaat atacttcgcg ttattttgat gaagctaacg gagcgcttta tccgttcggc
    43921 tatggtctga gctataccac tttcaccgtc tctgatgtga aactttctgc gccgaccatg
    43981 aagcgtgacg gcaaagtgac ggccagcgtg caggtgacga acaccggtaa gcgcgagggg
    44041 gccacggtag tgcagatgta cttgcaggat gtgacggctt ccatgagtcg ccctgtgaaa
    44101 cagctgaaag gctttgagaa aatcaccctg aaaccgggcg aaactcagac tgtcagcttc
    44161 ccgatcgata ttgaggcgct gaagttctgg aatcaacaga tgaaatatga cgccgagcct
    44221 ggcaagttca atgtatttat cggcactgat tccgcacgcg ttaagaaagg cgagtttgag
    44281 ttgctgtaat gctttagtaa gggcgcgacg tttgcgccct ttgcctgatg ttcatatctt
    44341 aacttgctga attttcgccc tttgtaggcc ggataaggcg ctcgcgccgc atccggcagg
    44401 aaacgccgga ccgaatatgc ctcttaagta tttatctgat tctgcaactg aatccttccg
    44461 ctcaggctaa ctctgtcatt atcaactatg cttttctctt aattcgctga aaacaacagt
    44521 aaaagaatga ggaaagcatc atgccactct taaagctctg ggcaggttca ctggttatgt
    44581 tggcagccgt gagcctgccg ctacaggcgg cttcccccgt taaagtcggt tcaaaaatcg
    44641 ataccgaagg cgcgctgctc ggcaacatca tcttgcaggt actcgaaagc cacggcgtac
    44701 cgacggtgaa taaagtgcaa ctcggaacga ctcctgtggg gcgcggggcg attacttccg
    44761 atgaactgga tatctatccg gaatataccg gcaatggcgc tttcttcttt aaagatgaaa
    44821 acgatgcagc gtggaaaaat gccgggcagg gctacgagaa agtcaaaaag ctcgatgcgg
    44881 agcacaacaa gttaatctgg ctgacgcccg cgcctgcaaa taacacctgg actatcgccg
    44941 tgcgtcagga tgtggcagag aaaaacaaac tcacttcgct tgccgacctg agtcgttatc
    45001 tgcaagaggg cggcaccttc aaactggcag cctcggcaga gtttatcgaa cgcgccgatg
    45061 cgttacccac gtttgaaaaa gcctatggct ttaagctcgg tcaggatcag ttgctgtcac
    45121 tggctggtgg cgacacggcg gtgacgatta aagccgctgc ccagcaaacc tctggcgtta
    45181 atgccgcaat ggcttacggc actgacggtc cggtcgcggc gctggggctg caaaccttaa
    45241 gcgatccgca aggcgtgcaa cctatctacg cgcctgcacc agtggtgcgt gaatcggtgt
    45301 tgaaggagta tccgcaaatg gcacagtggc tacagccagt cttcgccagc ctcgatgcaa
    45361 aaacattgca gcaactgaat gccagcattg cagtggaagg actggatgcc aaaaaagtgg
    45421 ctgccgacta cctgaaacaa aaagggtggg cgaagtaatt tcccgtgact tatctacgta
    45481 ttaatcctgt tctggcgctg ctgttgttgc tgacggcaat cgcagcggcg ctgccgttta
    45541 tcagttacgc gcctaatcgt ttagtctcgg gtgaagggct tcatctctgg caactgtggc
    45601 cgcaaacgat ctggatgctg gtgggcgttg gttgcgcctg gctgacagcc tgttttgtcc
    45661 ccgctaaaaa aggcagcatt tttgcactca ttctggcgca attcgtcttc gtattgttgg
    45721 tgtggggagc tggaaaggcg gcgacccaac tggcgcaaaa tggcagtgcg ctggcgcgta
    45781 ccagcctcgg cagaggtttc tggctggctg cggcgctggc attgctggcc tgtagcgatg
    45841 ccatccgccg aatctccacg catccgctgt ggcgctggtt gttgcatatg cagattgcca
    45901 ttattccgct gtggttgctg tactccggca cgcttaacga tctctcacta atgaaagaat
    45961 acgccaaccg tcaggatgtg tttgacgacg cgctggcaca acatctgacg ttgctgtttg
    46021 gtgcggtgct gcctgcgtta gtgattggtg tgccgttggg catctggtgc tacttttcca
    46081 ctgcgcggca gggggcaatt ttttctctgc tcaatgtcat tcagaccgtg ccttcggtgg
    46141 cgctctttgg cctgttgatt gcgccgcttg ccgcgctggt tacggccttt ccgtggctgg
    46201 ggaagctcgg catagcagga accggaatga cacccgcact gattgcgctg gtgctctatg
    46261 ccttgctgcc gctggtgcga ggcgtggtag tcggtttgaa ccagatcccg cgcgatgtgc
    46321 tggagagcgc cagagcgatg gggatgagcg gggcgcagcg tttcctgcat gttcagttac
    46381 cgctggcgtt accggtattt ctgcgcagcc tgcgggtggt gatggtgcaa actgtaggta
    46441 tggcggtgat tgcggcgtta atcggcgcag gcggttttgg tgcgctggtt ttccaggggc
    46501 tgctaagcag cgccattgat ttagtgttgc tgggggtgat cccggtaatt gttctggcgg
    46561 tgcttaccga cgcgctgttc gatttgctta tcgcactgct gaaggtgaaa cgtaatgatt
    46621 gaatttagcc atgtcagcaa actgttcggc gcacaaaaag ccgttaacga tctcaatctc
    46681 aattttcagg aagggagttt ttcggtgctg attggcacat ctggctccgg caaatccacc
    46741 accctgaaaa tgattaaccg cctggtggag catgacagcg gagtgatccg ctttgccgga
    46801 gaagaaattc gctcgctgcc agtactggag ttgcgccgcc ggatgggcta tgccattcaa
    46861 tctattggcc tgttccccca ctggagcgtg gcgcaaaaca tcgctaccgt gccgcaatta
    46921 caaaaatggt cgcgggcgcg gattgacgat cgtatcgacg aattaatggc gctactgggg
    46981 ctggagtcaa atttgcgtga gcgttatccg catcagcttt ccggtggtca gcagcaacgt
    47041 gtgggagtgg cgcgtgcact ggctgccgat ccgcaagtct tactgatgga tgaacctttt
    47101 ggcgcactgg acccggtaac gcgcggcgcg ttgcaacaag agatgacgcg cattcaccgt
    47161 ttgctggggc gcactattgt gctggtcact catgatattg atgaggcgct acggctggca
    47221 gaacatctgg tattgatgga tcacggtgaa gtggtgcagc aggggaatcc gctgacgatg
    47281 ctgactcgtc cggcgaatga ttttgtccgc cagttttttg gacgtagtga actgggggtg
    47341 cacctgcttt cgttacgtag cgtggcggat tacgtgcgtc gcgaagaacg ggcagaaggt
    47401 gaggcactgg cagaagagat gacgctacgc gatgcgctct ccctgtttgt cgcgcgggga
    47461 tgcgaggtgc tgccggtggt gaacacgcag ggccagcctt gcggcacgct gcattttcag
    47521 gatctgctgg tggaggcgta agcgtatgaa gatgttgcgc gatccgctgt tctggctcat
    47581 tgctcttttt gtggcgctga ttttctggct gccttacagc cagccgctgt ttgctgcctt
    47641 gttcccacaa ctgccacgac ccgtttatca gcaagaaagt tttgcagctc tggcactggc
    47701 tcatttctgg ctggtgggaa tttcgagttt gtttgcggtg atcattggca caggtgccgg
    47761 aattgctgtt actcgcccgt ggggcgcgga atttcgccca ctggtggaaa ctattgccgc
    47821 cgttggacag acttttccgc ctgtcgcagt gctggcgatt gccgttccgg tgatcggctt
    47881 tggtctgcaa ccagcgatta tcgccttgat cctttacggt gtgctgccca tcctgcaggt
    47941 gacacttgcc gggctgggag cgattgatcc cagcgtgaca gaagttgcga aaggtatggg
    48001 aatgagtcga ggtcagcgac tgcgtaaggt cgagctaccg ctggcggctc cggtgattct
    48061 ggcgggcgtg cgaacttcgg tgattatcaa cattggtacg gcgacgatcg cctcaacggt
    48121 aggggccagt acgctgggta cgcccatcat catcgggctt agcggattta ataccgcgta
    48181 tgtgatccag ggggcgttac tggtggcact ggcggcgatc atcgcagacc gcctgtttga
    48241 aaggctggtg caggcgctta gccagcacgc aaaataaagg tataacctgc gagcat
//
LOCUS       DAJTYL010000014        48000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYL010000014 DAJTYL010000000
VERSION     DAJTYL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.014"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(196..1386)
                     /gene="nepI"
                     /locus_tag="OO572_000820"
     CDS             complement(196..1386)
                     /gene="nepI"
                     /locus_tag="OO572_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6659918.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1597..2049)
                     /locus_tag="OO572_000821"
     CDS             complement(1597..2049)
                     /locus_tag="OO572_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6659919.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2102..3436)
                     /gene="adeQ"
                     /locus_tag="OO572_000822"
                     /gene_synonym="yicO"
     CDS             complement(2102..3436)
                     /gene="adeQ"
                     /locus_tag="OO572_000822"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6659920.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3611..5377
                     /gene="adeD"
                     /locus_tag="OO572_000823"
     CDS             3611..5377
                     /gene="adeD"
                     /locus_tag="OO572_000823"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6659921.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5423..6814)
                     /gene="uhpT"
                     /locus_tag="OO572_000824"
     CDS             complement(5423..6814)
                     /gene="uhpT"
                     /locus_tag="OO572_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6659922.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6952..8271)
                     /gene="uhpC"
                     /locus_tag="OO572_000825"
     CDS             complement(6952..8271)
                     /gene="uhpC"
                     /locus_tag="OO572_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6659923.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8281..9783)
                     /gene="uhpB"
                     /locus_tag="OO572_000826"
     CDS             complement(8281..9783)
                     /gene="uhpB"
                     /locus_tag="OO572_000826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6659924.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9783..10373)
                     /gene="uhpA"
                     /locus_tag="OO572_000827"
     CDS             complement(9783..10373)
                     /gene="uhpA"
                     /locus_tag="OO572_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6659925.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10448..10738)
                     /gene="ilvN"
                     /locus_tag="OO572_000828"
     CDS             complement(10448..10738)
                     /gene="ilvN"
                     /locus_tag="OO572_000828"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6659926.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10742..12430)
                     /gene="ilvB"
                     /locus_tag="OO572_000829"
     CDS             complement(10742..12430)
                     /gene="ilvB"
                     /locus_tag="OO572_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6659927.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12536..12634)
                     /gene="ivbL"
                     /locus_tag="OO572_000830"
     CDS             complement(12536..12634)
                     /gene="ivbL"
                     /locus_tag="OO572_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6659928.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13199..13288
                     /gene="tisB"
                     /locus_tag="OO572_000831"
     CDS             13199..13288
                     /gene="tisB"
                     /locus_tag="OO572_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6659929.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13568..14752
                     /gene="emrD"
                     /locus_tag="OO572_000832"
     CDS             13568..14752
                     /gene="emrD"
                     /locus_tag="OO572_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6659930.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14760..15257)
                     /locus_tag="OO572_000833"
     CDS             complement(14760..15257)
                     /locus_tag="OO572_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6659931.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15254..15616)
                     /locus_tag="OO572_000834"
     CDS             complement(15254..15616)
                     /locus_tag="OO572_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6659932.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15606..15953)
                     /locus_tag="OO572_000835"
     CDS             complement(15606..15953)
                     /locus_tag="OO572_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6659933.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16062..16475
                     /locus_tag="OO572_000836"
     CDS             16062..16475
                     /locus_tag="OO572_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659934.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16522..17964)
                     /locus_tag="OO572_000837"
                     /pseudo
     CDS             complement(16522..17964)
                     /locus_tag="OO572_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17999..19714)
                     /locus_tag="OO572_000838"
     CDS             complement(17999..19714)
                     /locus_tag="OO572_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6659935.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            19881..20774
                     /locus_tag="OO572_000839"
     CDS             19881..20774
                     /locus_tag="OO572_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6659936.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20771..21585)
                     /locus_tag="OO572_000840"
                     /pseudo
     CDS             complement(20771..21585)
                     /locus_tag="OO572_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21585..23201)
                     /locus_tag="OO572_000841"
     CDS             complement(21585..23201)
                     /locus_tag="OO572_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6659937.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23496..24170
                     /locus_tag="OO572_000842"
     CDS             23496..24170
                     /locus_tag="OO572_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6659938.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24209..25870)
                     /locus_tag="OO572_000843"
     CDS             complement(24209..25870)
                     /locus_tag="OO572_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6659939.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26066..26494)
                     /gene="ibpB"
                     /locus_tag="OO572_000844"
     CDS             complement(26066..26494)
                     /gene="ibpB"
                     /locus_tag="OO572_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6659940.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26606..27019)
                     /gene="ibpA"
                     /locus_tag="OO572_000845"
     CDS             complement(26606..27019)
                     /gene="ibpA"
                     /locus_tag="OO572_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6659941.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27013..27085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27324..27656
                     /locus_tag="OO572_000846"
     CDS             27324..27656
                     /locus_tag="OO572_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6659942.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27658..28890)
                     /locus_tag="OO572_000847"
     CDS             complement(27658..28890)
                     /locus_tag="OO572_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6659943.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            28991..30091
                     /gene="cbrA"
                     /locus_tag="OO572_000848"
     CDS             28991..30091
                     /gene="cbrA"
                     /locus_tag="OO572_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6659944.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30052..31389)
                     /locus_tag="OO572_000849"
     CDS             complement(30052..31389)
                     /locus_tag="OO572_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6659945.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31464..32612)
                     /gene="dgoD"
                     /locus_tag="OO572_000850"
                     /pseudo
     CDS             complement(31464..32612)
                     /gene="dgoD"
                     /locus_tag="OO572_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32609..33226)
                     /gene="dgoA"
                     /locus_tag="OO572_000851"
     CDS             complement(32609..33226)
                     /gene="dgoA"
                     /locus_tag="OO572_000851"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6659946.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33210..34088)
                     /gene="dgoK"
                     /locus_tag="OO572_000852"
     CDS             complement(33210..34088)
                     /gene="dgoK"
                     /locus_tag="OO572_000852"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6659947.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34085..34774)
                     /gene="dgoR"
                     /locus_tag="OO572_000853"
     CDS             complement(34085..34774)
                     /gene="dgoR"
                     /locus_tag="OO572_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6659948.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35052..35708
                     /locus_tag="OO572_000854"
     CDS             35052..35708
                     /locus_tag="OO572_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659949.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35754..36566)
                     /gene="yidA"
                     /locus_tag="OO572_000855"
     CDS             complement(35754..36566)
                     /gene="yidA"
                     /locus_tag="OO572_000855"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6659950.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36680..37078)
                     /locus_tag="OO572_000856"
     CDS             complement(36680..37078)
                     /locus_tag="OO572_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6659951.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37318..39732)
                     /gene="gyrB"
                     /locus_tag="OO572_000857"
     CDS             complement(37318..39732)
                     /gene="gyrB"
                     /locus_tag="OO572_000857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6659952.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39761..40834)
                     /gene="recF"
                     /locus_tag="OO572_000858"
     CDS             complement(39761..40834)
                     /gene="recF"
                     /locus_tag="OO572_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6659953.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40834..41934)
                     /gene="dnaN"
                     /locus_tag="OO572_000859"
     CDS             complement(40834..41934)
                     /gene="dnaN"
                     /locus_tag="OO572_000859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6659954.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(41939..43342)
                     /gene="dnaA"
                     /locus_tag="OO572_000860"
     CDS             complement(41939..43342)
                     /gene="dnaA"
                     /locus_tag="OO572_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6659955.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43639..43719
                     /locus_tag="OO572_000861"
     CDS             43639..43719
                     /locus_tag="OO572_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659956.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            43949..44089
                     /gene="rpmH"
                     /locus_tag="OO572_000862"
     CDS             43949..44089
                     /gene="rpmH"
                     /locus_tag="OO572_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6659957.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44106..44465
                     /gene="rnpA"
                     /locus_tag="OO572_000863"
     CDS             44106..44465
                     /gene="rnpA"
                     /locus_tag="OO572_000863"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6659958.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44429..44686
                     /gene="yidD"
                     /locus_tag="OO572_000864"
     CDS             44429..44686
                     /gene="yidD"
                     /locus_tag="OO572_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6659959.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44689..46335
                     /gene="yidC"
                     /locus_tag="OO572_000865"
     CDS             44689..46335
                     /gene="yidC"
                     /locus_tag="OO572_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6659960.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46441..47805
                     /gene="mnmE"
                     /locus_tag="OO572_000866"
     CDS             46441..47805
                     /gene="mnmE"
                     /locus_tag="OO572_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6659961.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11874 a        12995 c        12154 g        10977 t
ORIGIN      
        1 cacatgcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat gaaaacaata
       61 aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc aattattggg
      121 aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta ctgaccaacg
      181 cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca acaatgcagt
      241 cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat tatccagcgc
      301 ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca cctgaataga
      361 cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc agccgacggg
      421 aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga ttttatcgct
      481 tccccacaac gtcagtacca acgcactcac agccagtatt aacggcgcgc ctgctaaggc
      541 cagttttacc gaacgtttaa gaatgaacga cgaaagcgac gtaccaataa agctggcgat
      601 accaaaactc aacagcacca gcgttaagcc atccacgccg aatcccgcca ggttcatata
      661 caccgggcga atatacgtga agaaagcaaa ctgcccggcg aaagacatga agatggcgat
      721 catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt tctgatgcga
      781 gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca gcacgcccat
      841 caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa aactgcccaa
      901 cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca gcgccttcgg
      961 caccgtacgc ggcggtacca gacgcatggt cagcgacgcc gacatcgccc agaacccgcc
     1021 cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt tagcaaagga
     1081 aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt agcggcggtc
     1141 agtggcctga attgtctggg taataaacaa actggcaaac attgccacaa aggcggtcac
     1201 ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca ttggcgtcaa
     1261 caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg ccaccgagaa
     1321 aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg caataaattc
     1381 actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta acattgaaat
     1441 gtgtgacgaa tttaacgttt ttgcaacttt tacggtgttg cttgagctgg agttaagatc
     1501 gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca cccgacaggt
     1561 caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt gaacgccctc
     1621 ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct gcatttcagg
     1681 ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa tcggcgcgga
     1741 aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc tatcatcgac
     1801 aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg actcatccgg
     1861 gcgatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat cgaccatcga
     1921 ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg cttttctggc
     1981 cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg cgagcattat
     2041 ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc ctcgccagtt
     2101 actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc acacacaggt
     2161 tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata aagccgagtg
     2221 caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata aacgcaggca
     2281 ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc agcacgccaa
     2341 caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc ggtgagaaga
     2401 acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg cgaccaccga
     2461 ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg ataaatgcgc
     2521 ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg aatttaccgt
     2581 tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat gaatcaaata
     2641 ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac gcgccgctca
     2701 aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg ccgctaaaat
     2761 gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc agcaccgcgg
     2821 catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata cctaacaaca
     2881 cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg acaataacac
     2941 cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg ataccaatac
     3001 gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc gtgagcaaaa
     3061 atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag atccccatcg
     3121 cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc agcgccacgg
     3181 gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa caggtggtaa
     3241 caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc acaaaaacaa
     3301 tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg acggtggtcc
     3361 catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc acgtaatcgg
     3421 tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc ttttgtaaaa
     3481 caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt aatagatatt
     3541 ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat aaaatcaaaa
     3601 tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc gggctgaata
     3661 ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta ttgataatgt
     3721 ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga ttaaaggacg
     3781 ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc ggattgatgc
     3841 tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg aatccagcat
     3901 gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga ccgttatttg
     3961 cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt ttgcccgctg
     4021 tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc ccgctctgga
     4081 aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct ggcgggacca
     4141 tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta acgggcagaa
     4201 tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc actgtccggg
     4261 tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact gccacgaaag
     4321 ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga tgatccgcga
     4381 agggtccgct gccagcaatc tcaacgcact ggcaccgttg atcaacgaat ttaacagccc
     4441 gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg aagggcatat
     4501 cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg tggcatatcg
     4561 cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct tactggcacc
     4621 cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg tgcagcaggt
     4681 actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt cggcgagact
     4741 ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt ccgccagcga
     4801 ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca tccataacga
     4861 attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc gcgatgatgt
     4921 gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct gtggtttgct
     4981 cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg acagccataa
     5041 tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc aggtgattca
     5101 ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc tgccgttacc
     5161 cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa ttgacgcctt
     5221 gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc agatggcgtt
     5281 tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat ttgatggcga
     5341 gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg gcgtcatgcc
     5401 gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt ttctcgcggc
     5461 ggattttgcg ttcttccatt accgccacta tcgccatcag gcagatacaa ccaatcgcgg
     5521 cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc ggcgtcccat
     5581 cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa gcaaaggtgc
     5641 ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca gcagccacac
     5701 caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa gccagataga
     5761 tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc gccagcgcga
     5821 tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc cacagcagcg
     5881 taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc gctttagaga
     5941 gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata cgtaccacat
     6001 agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc acatactcaa
     6061 caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct tcgctgatct
     6121 cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag tcgctgccgt
     6181 aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata aacatgccga
     6241 tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct gcgcctgcac
     6301 cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta cgacgcggcg
     6361 tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa aagccgctta
     6421 aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata ctggcactga
     6481 agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt ttggtgtttt
     6541 tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg gagaaaccca
     6601 ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata tcgttctgcg
     6661 cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag accaccaggt
     6721 aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga aggtccaggg
     6781 tcggcttgcg aacttggttt aggaaagcca gcatgggtta ctcctgaaat gaatacctgc
     6841 gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt ccaagaaaaa
     6901 cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc atcacgcttc
     6961 acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc cggcggcgat
     7021 agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg ccagcggcca
     7081 accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg ccgcccctgc
     7141 cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg ggccaaagac
     7201 aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa atggcatcag
     7261 ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca tcggcccacg
     7321 gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga taaatccgcc
     7381 cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca gcgtctcgga
     7441 catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca gcacatagca
     7501 aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga tctctttacg
     7561 cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc gccattcacc
     7621 gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga gaaaaatccc
     7681 cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt aatgcagcgc
     7741 gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg tgttccataa
     7801 tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac gcgcacacac
     7861 cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa acgcccatag
     7921 cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc ctatccccat
     7981 aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg acacgccgta
     8041 agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca cgccgttagc
     8101 aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca gcgcgtaacc
     8161 cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat caatttcatg
     8221 tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca tcatcgaacc
     8281 ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac aggaaatgtg
     8341 taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa aaccttgttg
     8401 cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca accgttcatc
     8461 ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa tgttgttcag
     8521 cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg ctgattcatc
     8581 gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt cccgcatcag
     8641 tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta accgtcctaa
     8701 caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct gcccgctctg
     8761 cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag tacgaatagc
     8821 agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc gcacgctctc
     8881 ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct tttgtagcga
     8941 ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg tcagactttg
     9001 caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac tggcgatcag
     9061 cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt gccaggccag
     9121 cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt ccggcaatcc
     9181 caactggagc cagagactga taacaaacag cagcaagtac cagaccagat gccgcccgcg
     9241 ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt tattggcgag
     9301 atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta aagtcagcaa
     9361 cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca acaacgccgc
     9421 cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat ggcgatagcg
     9481 cgagatcagc gctacgggca gtaacgtcag caaactaccg atcatcaata acggaaaatg
     9541 ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac ccagcaatac
     9601 gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa acggaaataa
     9661 cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata ggcaaaacca
     9721 tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga acaacgtctt
     9781 catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac gcccagtttt
     9841 tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag ttcggcggca
     9901 atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc acgtttggtc
     9961 agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag ataacagccg
    10021 cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg tttggaaaga
    10081 aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc gtgaacggag
    10141 agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc ggagatatcg
    10201 ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc cgccagcgcc
    10261 tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc cagcagctgc
    10321 gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat catggtcttg
    10381 tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct
    10441 tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg gactgattac
    10501 gctgcacttt cacgacatct tccagcttag cgatttggct tatcatctgc tccagacgct
    10561 ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc ggcagacaaa
    10621 gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg gtcattacgc
    10681 ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg ttttgcatgg
    10741 cttattcccc caccatttca gtattcgccg cacctggcgg caccatcggg taaacttttt
    10801 cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatg atttcctgca
    10861 atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg aatccggcgg
    10921 caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct tgctcgtaga
    10981 acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga atgattttga
    11041 catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc aggctgccgt
    11101 cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca atcgccgcag
    11161 gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt gggcgattga
    11221 gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg ataatcgcat
    11281 tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat ggatcgcacg
    11341 ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg tgccactctg
    11401 cacgcggttg cgcttccacc cgcgggatca attgcgccag cacgtcatca acatccgcct
    11461 gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata tcgacatgaa
    11521 taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg
    11581 caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg cgcacgccgt
    11641 gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc agcgccatta
    11701 aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc gcaggcgcat
    11761 tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc attgtagctg
    11821 cgtcacgaat gctttcttcg ctaaaggcgg gtgcaacggc tttttctgcc accgcgggct
    11881 gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc cacaccgggc
    11941 tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg agctcttcga
    12001 tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag gtgtccactt
    12061 cttggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg caaatcagcg
    12121 ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca cccggcccgc
    12181 tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga gcgataaacc
    12241 ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg cttaaggcgt
    12301 cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt
    12361 ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg
    12421 aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa
    12481 aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc agtccctacg
    12541 gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat ggcgcagttg
    12601 gtagtagttt tgcgttgaac atggaagtag tcattgggga ccttgttggt tttgtgttta
    12661 acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt cagcaaggga
    12721 aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac cccgccggag
    12781 cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt atgtcaacac
    12841 tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg acgtatttag
    12901 tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt ttatttatac
    12961 agtaagcttc tataatatca ctgtacgcaa tgtgttatgc gggggccgca tcgttacccg
    13021 gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc gcgtgagcac
    13081 actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca caacagctgg
    13141 caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg agacgcgtat
    13201 gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac tgcaactgct
    13261 tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa ggggagcggg
    13321 taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg ctgacgcatc
    13381 ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta catattctgc
    13441 cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat gtgatagatt
    13501 cctctcccgc atttatggga atgcgtagtg acttattcta attattttta taaaagcatc
    13561 cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat tactcgtggc
    13621 cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg cgcgcgatct
    13681 caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga cttacggtgt
    13741 ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag tgatcctcgt
    13801 cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca gtttgacggt
    13861 attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg taatggcgcg
    13921 tactttgccg cgtgatttat atgaacggac acagttgcgc catgctaaca gcctgttaaa
    13981 catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc tggatacgat
    14041 gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg tgaccttcag
    14101 tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc gcctgcttac
    14161 cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga tgctgattgg
    14221 cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg gcgcggtgtt
    14281 agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg cggcattttt
    14341 tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt ggcagtcggt
    14401 tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg gcgtgatgaa
    14461 tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga tgctgtttcc
    14521 gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg ctggtgcgct
    14581 ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg cgatgttgcc
    14641 gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt tgatcgtgct
    14701 gtgctggctg ccgctggcga cgcggatgtc gcatcagggg cagcccgttt aagcgcacgt
    14761 caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag atcacgcatc
    14821 acccgcatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca aagcacgctg
    14881 tctggcgtct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc ctggcagtca
    14941 cggcgcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc aaaaagctca
    15001 atcgagatac gactgatatc tgcccgcacc caacactcaa acagggcaat taaaaatttc
    15061 ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc aaattcaaga
    15121 tagggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata aaaatcggca
    15181 ggttgcagag catccttgta ctctacaacc agcttatgcc tgtcctcttc agcatcaatg
    15241 acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt atatgcgtta
    15301 cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg tcacggacta
    15361 cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta cgggtgtagt
    15421 gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat aacatgcccg
    15481 cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc agcatggtac
    15541 gaaaccacgc cagtgatgtg cgctccggct gcagccctgg gtcggcaatg cgtctggctt
    15601 tacggctatc cggcatacaa caccagtccc ataacgatca ccgccacaac catcaatatt
    15661 aagctgatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg catcgctttt
    15721 tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa cagacacaac
    15781 aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag ttgatcaagc
    15841 ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa agtacgctca
    15901 ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt cattgttgct
    15961 cctttgcaat ttcactaacg ctctatcttt agcggttttt tttaagaatt tcgtccgccc
    16021 gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc atagcgcaaa
    16081 ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg gggattatcc
    16141 acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc gtggcggaca
    16201 aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa attaccactg
    16261 tggagaaaaa agaggacatg gatatcgata aaatcctcga tcaaagcggt atcgcactgg
    16321 ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat cgctggggca
    16381 ttcgcggcgc gctggttttt ggtatcggca tcgtgtgcag cattttgctg atattcctga
    16441 ttttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg cccgccgggt
    16501 gatcatcagc gggcattatc gtcagaattt ctgttttttc tcctccacct tcactccttg
    16561 cgtcggtagg ccggtgtcat agtcgcgtac cactggcgaa tagccatctt gtgggcgtgg
    16621 gcgaaacgcc cccatccagc gcggtcgtgc atctttacgc cacggacgca gactccattg
    16681 gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt catgcatttt
    16741 gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat ttgggtcatt
    16801 acggcgatcg taaagttcat cactggtgaa gaggttgagc accagtttaa agtcatccgt
    16861 cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct cgtagcggtt
    16921 aaattccacc atcacgccgc gtggctcttt cacggcaagg atattttccc ccggcagaat
    16981 ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcaa tatgactgac
    17041 tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg gaatacgggt
    17101 gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca tcatttcacc
    17161 gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta aggcattgat
    17221 gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg gatggtgata
    17281 aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt gatgttccgg
    17341 tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat cagcgtattt
    17401 ctctaaatac tccaccggac aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat
    17461 caggaagggt tcctcggcgc gcacgggcta ttgcagaaaa tctaccgctc gattgctgat
    17521 gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt cgacgctgtt
    17581 taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat agttcgcccc
    17641 atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga aatagtcatg
    17701 accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct taaagtagcg
    17761 ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc cggactggtt
    17821 agcgtagata ccggtgaata gtccggcccg tgcaggcgta caaaccggtg aacaagtgta
    17881 ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg tattcagcgg
    17941 tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt ggggcgtttc
    18001 ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga tgccaccagc
    18061 tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc atcgctaacc
    18121 agcgcgtacc gtagccgccg aattcagcca gcccgacata gacgccaatc atcgcgaaca
    18181 ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc gcaaacgcat
    18241 ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc atcacgacca
    18301 cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac ttcaccagat
    18361 agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc gccagcgccg
    18421 ggatgcgcgg aaagaaaaag cccataatga tgatggtaac cagcggcacg ttgtaaatgc
    18481 cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttcgcgatc cacggcgcta
    18541 ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg accagctgct
    18601 gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat aatgtactgg
    18661 cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc gcgccgaaga
    18721 aaccctccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc atgtcggctt
    18781 tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt accagcgggt
    18841 cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc aggctttttg
    18901 acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg ttcaccagaa
    18961 ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg actgagttta
    19021 atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg cccttgccta
    19081 tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca ataccgttga
    19141 tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa attcccgcca
    19201 gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt agcgattccc
    19261 cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc ggcagaaagc
    19321 agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt ttatcataac
    19381 gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc ggtaaaaaga
    19441 ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc accgacatgc
    19501 cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg ttggttaaca
    19561 ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa tagccttgtt
    19621 gcgatccggt atctgttttg cgaaccttcc accaggtgat caccgccacc agcagcgtaa
    19681 aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt atgtttatgt
    19741 cccggacgca tgaatatagg gagaggtaaa atgcgtcggg tcaggcagtc agattcttaa
    19801 aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt gtgagcataa
    19861 ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta aaaaacgaaa
    19921 ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga atttctctga
    19981 tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg cgggacaaga
    20041 agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga tgtgggaaaa
    20101 ttcttttaaa gaatggcgaa caaataactc tgcatagcaa ctgtattata tttttaaagc
    20161 caatggatat tcactcttat cactgtgaag gtttagtctg ggaacagtac tggatggaat
    20221 ttacccccac cagtatgatg gatatttccg ttggtcagca aagcgttatt tataatggcg
    20281 aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca gaagcaataa
    20341 aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt tgtcttatgt
    20401 ccgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt gccactttac
    20461 atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc ccctgtagcg
    20521 aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa gaatattacc
    20581 tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac tcggttggcg
    20641 aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc tttaaacata
    20701 aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca acggatgcca
    20761 gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc aactcatcca
    20821 gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc gcctgccaca
    20881 ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc gccacttgct
    20941 gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt tcaggccgta
    21001 gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca tccggctttc
    21061 aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca gacgacgcgt
    21121 ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt ttggtgaata
    21181 tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc cgtacgctat
    21241 tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga ttttttcatc
    21301 cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat caacgtcgct
    21361 gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt tttctttaag
    21421 gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat cataaaattt
    21481 cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga tgcctggcgt
    21541 aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac tgcctccgta
    21601 atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg ttcacgcaat
    21661 tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa gacgccgtgc
    21721 gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg cagtgcaata
    21781 cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc cagggcttgc
    21841 aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt ttggcctcgc
    21901 gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg tcccggcgtg
    21961 cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag ggtaaagcac
    22021 aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat cgggatccag
    22081 ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac accaaagaga
    22141 tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag caacggtgaa
    22201 atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc ggttaaggtt
    22261 gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt gaagtacatc
    22321 gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc aaaaccgcct
    22381 tccgggaaca acgatttcag tggctcggcg ctcagactga actcttgcag atgctgcgcc
    22441 cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg tccgtagatg
    22501 aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac ccacacccca
    22561 agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg tacttttggc
    22621 cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat aatcaccaca
    22681 taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa cagacggtta
    22741 tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt atcgagggtt
    22801 ttaatcccgg ccatcattgt cagaccgcta cctgccaccg cgtcctgagt gaaatcgacg
    22861 ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca ggtcaggaaa
    22921 ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc aatgggtaaa
    22981 ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc ttcaataatg
    23041 tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac aaacatcggg
    23101 ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag cagcactggc
    23161 gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc ctcttttctc
    23221 aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag gataggaaga
    23281 tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg cttttcgttg
    23341 ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag gtacacagca
    23401 tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact tgtgggcgca
    23461 ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc attgcggagc
    23521 ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cagccttctt cccggtgaaa
    23581 aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc attgacctgc
    23641 tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg gtgcgcaaag
    23701 atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa cggcagggaa
    23761 aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg gccattgcca
    23821 gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg
    23881 aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc cgtaaccttt
    23941 cgctgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc gggattttga
    24001 ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg gcgcggcaaa
    24061 tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat agcgagagcg
    24121 gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag gtggagtacc
    24181 atttacggcg actccacccg gaaaaaagtt aaccgatact ccagaagagc accgccagta
    24241 attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg taagagagcg
    24301 ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga ggatcggtca
    24361 tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac atccgcgcca
    24421 gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca atccagctta
    24481 gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga ccaacgaccg
    24541 agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc ggcataaacc
    24601 agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc agcggaccgc
    24661 ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga atagaaccta
    24721 acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt tgcagttttt
    24781 gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc cccaccaggt
    24841 tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg accccggcac
    24901 ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt
    24961 ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg
    25021 tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt aaatccgctg
    25081 gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc gaaggtactt
    25141 ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg agaatcggta
    25201 catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg atagtcttga
    25261 tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc tcgacattga
    25321 cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg ccgaatggat
    25381 acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta cccaggtcgc
    25441 gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg gagaaaatcc
    25501 ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta accagaccac
    25561 cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg
    25621 caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc aggccaaatt
    25681 cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa acaaaatggc
    25741 cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg cgaaatttga
    25801 cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc gttaatgcta
    25861 tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat tatggctgaa
    25921 tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt gccgaaatat
    25981 cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag ccccaccaga
    26041 atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg ctgatagcga
    26101 tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc agtaaaccgt
    26161 ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc aggctaaatg
    26221 gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc tccggcgtgc
    26281 cttttacgct caggcgcgtg ccttccagtt gaatctctaa atcttcctga cggaaacctg
    26341 ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg tacggcggga
    26401 agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt
    26461 gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct aagaagcgag
    26521 taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat ggagatgtca
    26581 ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt tttttcgctt
    26641 ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta gccccacgaa
    26701 catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg atgccctggt
    26761 acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc agcagattat
    26821 cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata gcaatgcggt
    26881 aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta ctctggctct
    26941 ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg taaagcgggg
    27001 ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt aagaccttcc
    27061 acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg actacgatgt
    27121 aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat
    27181 ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacagt
    27241 gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc
    27301 cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca
    27361 gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg gggaaagaag
    27421 gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa accaactggg
    27481 gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtaatggac acgcttttac
    27541 tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa aaaagcgaag
    27601 ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat aactgattta
    27661 gcgatcagtt tcggtgatcc acagctggcc accttccatc cacgccagcc attgaccatc
    27721 cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc
    27781 aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa agcccaacca
    27841 ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactt
    27901 gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatcacg
    27961 cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc gcgggacgtt
    28021 gactaacccc ggataagccc gatgatgggt aaaggttaaa cgtcgctgca cgacgccacg
    28081 cggtggcgcg ggtagggttg tttccgttcc acttaacggc gcatcacctg ccgatttcca
    28141 gctagcttca tcttgcggta actcaacgat aaatagctcc ggcactttct cgccctttgg
    28201 cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc
    28261 acgattgatt tcatcactgc caggctgcgg cgtgggcgtg gttttactca ccagcacaca
    28321 ccagtggcta ccgctgtatt cacgcggatg ttgcttttgt acgttgaccg ggccaaacgg
    28381 tgcagcaacc ccgacgtttc gcaaatccag cgccggatcg agttgatgca ttacatggtc
    28441 gttataggta aagctccccc tttcaccgtt cgggctaaag acatgcacat ggctgccacc
    28501 gcgcagcgct cctggcgtat acggcgcggt aatatccatt gcatcgagat tgctcacctt
    28561 gccgccttca gcaatgactc cgcgtcgatg atggaagtcg taatgccatg tttcatcagg
    28621 attttcaggg ccgtgaataa agacatattt ctctgacttc ggatgaacgg tcaccacgcc
    28681 gacgtgtgcg ccctgtgacg cgcgatagat aacctcgacc tcgcctgtat ggatattcac
    28741 acgctcaatg gtctcgccgg taaacgacgc cccagaagga cgcacgtcaa ataccagcca
    28801 ctggctgtcg ggcgtccagg tattggtatt tgtattggta ttggtattgg tgagcaggtg
    28861 attacgggga gcaaaggtta tctgtttcat ggcgcggtcc tgatacaaaa cggttcccgc
    28921 catcatactt cacaaggact tcactttcag acaatagtct tttgccatcg tgatgaatgt
    28981 gagtgaaaag atggaacatt tcgacgtggc gattattggc ctcggcccgg cagggtcggc
    29041 gttggcacga aagttagccg gcaaaatgca ggtgatcgcg ctggataaaa agcaccagtg
    29101 cggtactgaa ggtttcagca aaccttgcgg cggtctgctg gcaccggacg cgcagcgatc
    29161 ttttattcgc gatggactga cgcttcctgt cgatgtgatc gccaatccac agattttcag
    29221 cgtcaaaact gtcgacgtcg ccgcatcgct cacgcgtaac taccagcgaa gctatatcaa
    29281 tattaatcgc catgctttcg acttgtggat gaaatcgctg atccccgcca gcgttgaggt
    29341 ttatcacgat agcctgtgcc gaaaaatctg gcgtgaggat gataaatggc atgtcatttt
    29401 tcgtgcagac ggctgggagc aacatattac cgcccgctat ctggtcggtg ccgatggcgc
    29461 aaactcgatg gtgcggcgac atctctaccc ggatcatcaa atccgtaaat atgtcgctat
    29521 ccagcagtgg ttcgcagaga aacatccggt gccgttctac tcctgcatct ttgataatgc
    29581 gataactgac tgttactcat ggagtatcag caaagatggt tattttatct ttggcggtgc
    29641 ctatccaatg aaagacggtc agacgcgttt cacgacgctg aaagagaaaa tgagcgcctt
    29701 tcagttccag tttggcaaag cggtgaaaag tgagaaatgc acggtgttgt ttccctcacg
    29761 ctggcaggat tttgtctgtg gtaaggacaa cgcctttctg attggtgaag cggcgggatt
    29821 tatcagcgcc agctcgctgg aggggattag ctatgcgctg gatagcgcag agattctgcg
    29881 tgcggtgttc ctgaaacagc cggagaagag caacgccgct tactggcgcg ccacacacaa
    29941 actgcgttta aaactcttcg gcaagatagt aaaaagccga tgcctgaccg caccggcttt
    30001 aagaaagtgg attatgcgca gtggtgtggc gcatattcca cagttgaaag attatccaac
    30061 gcgcttcaca tcacccacca gcaggatgta agagagcgcg ccaatcaacg cgacggcgga
    30121 gatataaacc agtgcaggcg cgaaaccgta accctgcgcc aggtacccca ccaccagcgg
    30181 aacggtgatg ccgcccagac caccggcgaa gttaaacacg ccgccggtta aaccaatcag
    30241 gcgcatcggt gccagagaag agaccagcga ccaggtaatc gaagcaaaac cgttgccgaa
    30301 gaatgccagc gccatcaggc acataatcat catcggatcg ttggtgtagt tagcgcccat
    30361 aatgcaggtg gagatcagca agccgcagat aatcggcgtt ttacgcgcaa agcccagtga
    30421 aaagccctta cgtaccagca gatccgcgac ccagccagag agcaggacgc cgacaaacgc
    30481 cgcgaggaat ggcaccgtgg tcataaagcc tgctttcagc gccgtgattc ctttttcctg
    30541 ggttaaatag ttcgggaacc aggttaagaa aaaccacagt gcagaagcca ccgcaaattg
    30601 cccaagataa acgccgatca gtttacgatg gaacaccagt ttccagtctt tggctgttaa
    30661 cggctgacgc gcctctttct tcaccggcgc atcaccatcc accagaccgc cgccatcacg
    30721 aatgtaatcc agttcagctt tgctgatacc tttggtcagg cgcggcggct gataaacctt
    30781 aaaccaaatc agcgaccaga taatgccgat accaccagtg acaatgaaca cccagtgcca
    30841 gctcaacatc tcctgaatcc agatcagcag cggagtcaaa aacgccagac cgacaaactg
    30901 accagacgta taaaaaccaa cggcggaagc gcgttcatgt tccgggaacc agctggtcac
    30961 catccggtta ttggtcggga aagcgggcgc ttcgaaaata ccggttatcg cacgcagacc
    31021 aattaatgac attaatcccg tggcaaagcc ttggaataaa gtcgccactg accaaccaaa
    31081 tatcgcaata aaataagtca cgcgagaacc tacgcgatct aaaaaccaac cgccggggat
    31141 ctggcatagc gtataaagcc aggcgaaggc cgaaaataca tagcccattt ccgctttcgt
    31201 aatgccgaac tcttcctgaa tatgggcgga agccacggcc aggttagcgc ggtcaacata
    31261 acaaatgacc accgtaataa agatcatcac cagcgtcaga taacgccgac gccccggctt
    31321 tgctgcatta acgggaatat ccatagcgag ctttctccag attttgggca tagcgaagcc
    31381 gctcaccatg ccctgtaatt tacagagggt tatatttttg tattgctgtt ttagtgcccg
    31441 atgaggggct tacgtggcag gaattaccac tctgctacgc tgttatcttc atgacgccag
    31501 agcggattac gccagtccgg ggcattttta ctgaactcaa taactttagc ttcgtcgatt
    31561 tccacaccta agcccggttt cgttaacggt ttaaagaagc cgccaaccat gctgaagtct
    31621 tctttgtttt tcacaaagtc gagtaactcc gcgcctttgt tgtaatgaat gcccatactt
    31681 tgttactgaa gtaccgcgtt ataggaaacg aagtcgatat gcaggcaagc cgccagtgca
    31741 atcggtccga acggacagtg cggcgcaagg gtcacatcat aggcttctgc cattccagca
    31801 attttgtagc attcggtaat accgcctgca tgggagagat ccggttgcag aatcgaaata
    31861 ccgcctgcct ccagcacgcg tttaaaatcg aaacgtgaga acatgcgctc acccgccgcc
    31921 agtggaatat gcgtttgtgc cgccaatttc gggtagtatt cggcttgttc cgccagcacc
    31981 ggctcttcaa taaacagcgg gcgatacggc tccagttctt taatcagcac tttcgccatt
    32041 ggcgcgctga cgcgaccgtg gaaatcaaga ccaaactcaa tctgattgcc aaaagcttca
    32101 cgaatttgtg ccacggtgtt gactgccgca tctaccgcgc gggagttatc aattagcccc
    32161 agttcttcac aaccgttcag tttgaaggta tcgaagccga tttcgcgcag ggttttaatg
    32221 ccgtcgataa catccgccgg acgatcgccg ccgacccaac tgtaggcttt aattttgtcg
    32281 cgaaccaggc cgcccatcag ttgccagact ggcgcattca gcactttgcc tttgatatcc
    32341 cataacgcct ggtcgatccc ggcgatggcg ctcatcagga ttggaccgcc acgataaaat
    32401 ccggcgcgat acatcacttg ccataagtcg ttgatgcgcg aaggatcctg accaatcaaa
    32461 tagtcaccca gctcgtgaac tgccgcttcc accgtacggg cgcggccttc aatcacgggc
    32521 tcgccccaac cgaccacgcc ttcatcggtt tcaattttca ggaacatcca gcggggaggt
    32581 aaacgatacg tggtaatttt ggtgattttc attgcactgc ctctcgatac gccttaacaa
    32641 atgctgctgc ctgctgcgcg gtgcgttcta cggactgccc ggcgcgataa agatcgctgc
    32701 ctaagcccgc cccaacacaa cctgcgtcta tccactgcgc caggttttct ggcgtcacgc
    32761 cgccaacggc aaagactgcg atgtccggtg gtaataccgc ttttaacgct ttgatgtatt
    32821 gcggaccaaa agccgatgac ggaaatattt tcagcgcctg cgcgcccgct tcgagcgcgg
    32881 taaaggcttc ggtcgccgtc gcgcagccgg ggcaaacggt catgccgtag cccaccgcgc
    32941 ggcggatcac ttcactatgg atattgggcg taacgatgag ctgacagccc atcctggcga
    33001 gcgcatcgac ctgttcaggt ttcagtaccg tacctgcgcc aatcaacgcc ttgtcgccat
    33061 acgcatcaac gatggcggga atgctctgct cccattgtgg ggaattcagc gggatttcaa
    33121 ccgcgtcgaa cccggcgtca atcaccgcgc caacatgcgc cagcgcctcg tcgggcgtaa
    33181 tgccgcgcaa aatggcgatc agcgggagtt tagtttgcca ctgcatgagc gatgctcctt
    33241 ataccagcct gaaatgccgt gtcgcccgcc accgccgcca cgtcgcaacc catcgcctga
    33301 aaggcttgct ggtagcgcgc ggtcagcgat gttccggcga caagggtgat ggcgtgttga
    33361 tgggtcacat agtcgcgcat actggcaacc tctgcgccaa tcaacaaacc agagagaaat
    33421 tcgctgacct gttcgcgggg aagtgttccc agcacatgcg aggcgcgaac ttcaaaaagc
    33481 tgcggcaata tggcgggcgc attaaggccg cgctcaaggc cagccgcgaa ggcatcggca
    33541 gagttttcct gcggcggcaa acctgcgcca atcaatgagt gatttaacag taaatgatgt
    33601 aattcaccgg tcatcacggt gcgaaaatcg ttgatttgct ggctatcggc ctgcacccat
    33661 ttgcaatgcg taccgggcat aacataaaga gaggaaggag ccagagctcg cgcgccgatc
    33721 aattgtgttt cttcgccgcg catcacattg tggttatcgt catgagagac gcataatccg
    33781 ggaataatcc agatattgtc gccaacagac gttaattgtt cgccaataga cgaaaaacgg
    33841 gcaggaacag ataaatacgg agcaactttc cagccgacat tgctgccgac cattcctgcc
    33901 atcaccactg gcgtattttc ttcacgccag tcggtcgtga cttctgctaa cactgcagcc
    33961 ggagattttc cgttcaggcg cgtgacgcct gcttctgatt gcctgctctc caggcagtgg
    34021 tcgccctgat aaagccaggc gcgcagattg gtcgatcccc agtcaattgc gatgtagcga
    34081 gctgtcatgt gatttccttt aaccttcgtg tcgagctggc aatcatggta agcgccgcct
    34141 gctctgccgc atcgccgtcc tgatgccgta tcgcatcgaa cagcgcctta tgttcctgga
    34201 gcgtttgcgg catgttggcc tcatcgccca tccaggttcg ttcaaaaacc gcccgctgca
    34261 gcgaactgat cgcaatgcta agttgctgta acaccgggtt atgcaccgac tgcagcaccg
    34321 cctcgtggta gcgaatatcc gcttcgttaa acgcttcgcg gtcctgattg ttggcaatca
    34381 tctcgttcag cgccgattca atctgcgcca gatcgctgga ggtcgcgcgc tctgctgccc
    34441 aacgggcaat cgccggttcc accagatttc gcacttcgct catggcactg ataagccgtg
    34501 ggtcgtagtc gttttccagc acccattgca gtacgtcagt gtcgaggtaa ttccactggt
    34561 tacgcggtgc cacaaacgcc ccgcgataac gtttcatttc aatcagccgc ttcgccatca
    34621 gcgaacggaa cacctcacgg atgatgttgc gcgaggttgc aaactcttca cagagctccg
    34681 cctcagccgc aagtggcgag ccaggtacgt atttgccgtg aacgatctgt ttacccagcg
    34741 taatgacaat gcgatcggtt ttattgagag tcatggagag tccttgtgct ctgtatgttc
    34801 ttctctactt taccccgatc gatgcataac gcggcaactt tgtagtacca gcgtgatgac
    34861 gttcgcgttt gccgcgcgcg taatgtagta caaacttata ttgttgcgct acaatttaga
    34921 tcaaaaaaag aacaacacat aaaaaaatga catgcgtcgg gcagaaatct gaaaagggat
    34981 atcaggcgct aaacaggagg gaaagaagag tatgctttca acggcttagc tactcgttta
    35041 aaggattaat catgaagttg aattttaagg gattttttaa ggctgccggt ttattcccac
    35101 tggcgctgat gctttcaggc tgtatctcgt atgctctggt ttcccatacc gcaaagggta
    35161 gttcaggaaa gtatcaatcg cagtcagaca ccatcactgg gctatcgcag gcaaaagata
    35221 gtaatggaac aaaaggctat gtttttgtag gggaatcgct ggattacctt atcactgatg
    35281 gtgccgatga catcgttaag atgctcaatg atccagcact taaccggcac aatattcagg
    35341 ttgccgatga cgcaagattt gttttaaatg cggggaaaaa gaaatttacc ggcacaatat
    35401 cgctttacta ctactggaat aacgaagaag aaaaggcact ggcaacgcat tatggttttg
    35461 cctgtggtgt tcaacactgt accaggtcac tggaaaacct aaaaggcaca atccatgaga
    35521 aaaataaaaa catggattac tcaaaggtga tggcgttcta ccatccattt aaagtgcgat
    35581 tttatgaata ctattcaccc agaggcattc cggatggtgt ttccgcagca ttactgccag
    35641 tgactgttac actggacatc attactgcac cgctgcaatt tctggttgta tatgcagtaa
    35701 accaataatc agtaagcggg caaacgcgtt tatgctgttt gcccgcccac agattaattc
    35761 agcacatact tctcaatagc aaacgccacg ccatcttcaa ggttggattt tgtgacaaag
    35821 ttcgccactt ctttcaccga aggaatagcg ttatccatcg ccacaccgac gcctgcatat
    35881 tcaatcattg cgatgtcgtt ttcctgatcg ccaatcgcca tgatttcttc cggtttaata
    35941 cctaacacgt cggccagtga tttcacaccc gtacctttgt taacgcgttt atcgaggatt
    36001 tcgaggaagt acggcgcact tttcagcacg gtatatttct ctttcacttc ctgcggaata
    36061 cgcgcgatag cctggtcgag gatggcgggt tcatcaatca tcatcacttt caggaactgg
    36121 gtattggggt ccattttctc cgcttcgcag aacaccagcg gaattgtggc aacgaaggat
    36181 tcatgcaccg tgtagtagct gatatcgcgg ttagcggtgt acagcgtggt gcggtccagg
    36241 gcgtggaaat gagaaccgac ttcgcgagag agtttttcca ggaaacgata gtcgtcatag
    36301 ctgagagcgg tttgcgccac ggtgctacca tcagcggcct tctgtaccag cgcgccgtta
    36361 taagtaatgc agtagtcgcc cggctgttcc atatgcagct ctttcaggta gttgtgcaca
    36421 cctgcatacg gacgacccgt cgttagcacg acattcacgc cacgggcgcg agctgcggca
    36481 atcgcatttt taacggcggg tgaaatggtg tgatcgggca gcagaagggt gccatccata
    36541 tcgatagcaa tgagtttgat agccatgagt tcccaaggta gattagttcc tgacccatgc
    36601 taacgcgatt ccgctcaaaa atcagtacaa cacccgaggg aaaaggggat gcaacgcggg
    36661 ttgcatgctc cccatttgct tagcggaaga gtttcccttt cagcagttcc atgcctgcgg
    36721 aaagcagatc gttattggct tgtgctgaca cttcaccttg cggtgagagc gcatcaataa
    36781 tcttcggcaa ttgctctgcc agtaaactgg aagccgtact ggtatccacg ccaagttttt
    36841 gcccgagatc ggacaccgca tttgtgccga gcgccgactc cagttgctcg ccgctaactg
    36901 attgatttct ctgttgatta ctcaaccagg ttgagagaat ggcccctaag ccgccacttt
    36961 gcagtttttc cagcagcacc tgaatgccac cctgctcctc aacccaactt aaaatcgcct
    37021 gatatttccc cgcatcgcct ttcagaaagg caccgacaac ttcatcaaaa agccccatga
    37081 taatcacctg taaagcgtta cgtgttgacc caaaaagtat agatctgcgg gatgataatt
    37141 gcggattgca gaaataaaaa gggcggagat gatctccgcc cttttcttat agcttcttgc
    37201 cggatgcggc gtgaacgcct tacccggcct acaaaatcat gcaaaatcaa tatattgcag
    37261 gagtctcgta ggactaataa gcgtagcgca tcaggcacgc tcgcatggtt aacgccatta
    37321 aatatcaata ttcgccgctt tcagggcgtt ctcttcaata aacgcacgac gcggttctac
    37381 ggcgtcgccc atcagcgtgg tgaacaactg gtcagcggca atcgcatcct taacggtaac
    37441 gcgcagcata cgacggcttt ccgggtccat ggtggtttcc cacagctgtt ccgggttcat
    37501 ctcgcccaga cctttataac gctggatgga gaggccacga cgggactctt tcaccagcca
    37561 gtccagcgcc tgctcgaagc tggctaccgg ctgacgacgc tcgccacgtt cgataaacgc
    37621 atcttcttcc agcaagccac gcagtttctc acccagcgtg cagatacgac gatattcgcc
    37681 accggtgata aactcgtgat ccagcggata gtcagtatcc acaccgtggg tacgcacgcg
    37741 aacaatcggc tcgaacaggt tttgttcggc attggtgtgg acatcaaact tccactggct
    37801 gccgtgctgt tctttgtcgt tcagttcgct gaccagcgcg ttcacccagc gggtaacggt
    37861 ctgctcatca gaaaggtcag cttccgtcaa cgtcggctga tagataagct ctttcagcat
    37921 tgctttcgga taacgacgct ccatgcggtt gatcattttc tgcgtcgcgt tgtactcaga
    37981 taccagtttc tctaacgctt cgccagccaa tgccggtgca ctggcgttgg tgtgcagcgt
    38041 tgcgccgtcc agcgcgatag agatctggta ctgatccatc gcttcgtcgt ctttaatgta
    38101 ctgttcctgc ttgcctttct tcactttgta cagcggcggc tgagcgatgt agacgtgacc
    38161 gcgttcaacg atttccggca tctgacgata gaagaaggtc aacagcagcg tacgaatgtg
    38221 cgagccgtcg acgtccgcat cggtcatgat gatgatgctg tgataacgca gtttgtccgg
    38281 gttgtactcg tcacgaccga taccacagcc aagcgcggtg ataagcgtcg ccacttcctg
    38341 agaagagagc atcttatcga agcgcgcttt ctcgacgtta aggattttac ccttcagcgg
    38401 cagaatcgcc tggttcttgc ggttacgccc ctgcttcgca gagccgcccg cggagtcccc
    38461 ttccaccagg tacagttcgg aaagcgccgg atcgcgttcc tggcagtctg ccagtttgcc
    38521 cggcaggcca gctaagtcga gcgcaccttt acggcgggtc atttcacgcg cgcgacgcgc
    38581 cgcttcacgg gcacgggcag catcgataat tttgccaacc acgattttcg catcagttgg
    38641 gttttccagc aggtattccg ccagcagttc gttcatctgc tgttcaaccg ccgatttcac
    38701 ctcagaagaa accagtttgt ctttggtctg ggaggagaat ttcgggtccg gcacttttac
    38761 ggaaacgacc gcaatcaggc cttcacgcgc atcgtcaccg gtggcgctga ctttggcttt
    38821 tttgctgtag ccttctttgt ccatgtaggc attcagggta cgggtcatcg ccgcacggaa
    38881 gcctgccagg tgagtaccgc cgtcacgctg cggaatgttg ttggtaaagc agtagatgtt
    38941 ttcctggaag ccatcgttcc actgcagcgc cacttcaacg ccaataccgt ctttttcggt
    39001 ggagaagtag aagatatttg ggtggatcgg cgttttgttc ttgttcagat attcaacgaa
    39061 cgctttgatg ccgccttcat agtggaagtg gtcttctttg ccgtcacgct tgtcgcgcag
    39121 acgaatggaa acgccggagt tgaggaacga caactcacgc agacgtttcg ccagaatttc
    39181 atattcgaac tcggtcacat tggtgaaggt ttcgaggctg ggccagaaac gcaccatggt
    39241 gccggttttt tcagtctcgc cggtaaccgc cagcggagcc tgcggtacac cgtgttcgta
    39301 gatctgacgg tgaattttac cctcgcgctg aataaccagc tccagttttt gcgacagggc
    39361 gtttactacc gaaacaccaa cgccgtgcag accgccggac actttatagg agttatcgtc
    39421 aaatttaccg cctgcgtgca gaacggtcat gatcacttcc gccgccgata cgccctcttc
    39481 cgggtgaata ccggtcggaa tgccgcgccc gtcatcctgt acagagacag agttatcggc
    39541 gtgaatggtg acgataattt ctttacagtg acccgcgagc gcttcgtcga tagcgttatc
    39601 taccacctcg aataccatgt ggtgcagacc ggtgccgtca tccgtgtcgc cgatatacat
    39661 acccgggcgc ttacgcaccg catccagccc tttcaggact ttgatactgg aggagtcata
    39721 agaattcgac atcaacgttt ctcgctcatt tatacttggg ttaatccgtt attttaccct
    39781 tttccacggt aaacatcttc gaattttcgt ccgacatgtc tataacgtgt tcagcactga
    39841 tcgcgctgac aaagacctgt gattgcgtcg cttttaagcg gctggcaagc aacccgcgac
    39901 gctcatcatc aagctcagag gcaaaatcat ctatcaggta gagacaccgc cgcccgcttt
    39961 cacgagtgag gaactctcct tgcgccagac gtaaggcgca catcaacagc ttaagctgcc
    40021 cacgcgataa ggtatcttcc accggcgcac cgtcggcgcg aatgcgtaag tccgctttat
    40081 gcgggccatg cgcggtgtag gttagctggc gatcgcgttc aaaattacgt tccagcacct
    40141 cagcgtactc tgtctctttc tcccagccgc gctggaaaga gaaagtcaga gaaaactcag
    40201 ggagaaattg cttacaggta tcatccatat cggccgcgat accggcgcta tactccgcgc
    40261 gccaggtgct gatttgctcc gccagcggga tcagttcttt atcccacggg cgtagctgtt
    40321 cgtaacgagt cacctggcgc agcgccgcat tgcgctgctt gagcaatcgc ttgagattgc
    40381 tccaggcggt gaaaaatcca ggttcgttgt gaaagcatcc ccagtcgagg aatgctcttc
    40441 tgtatttggg gccgccgttg agtaaagtaa acccttctgg cgttatcaac tgcatcggca
    40501 tcaggtgcgc cagttccgcg actttatgcc cgtccgtacc gtcaatacgg actttgctgt
    40561 cgccctgttt gtctttggtt aagccaatcg ccgtctcgcg ctcttcgccc tgtaatcgcc
    40621 cgtggagaac aaatgcctcc tgctcatggc gaatgacgcg accaatctgc aaactgcgaa
    40681 acgcccgacc atggccgagc gtatagatgg cttccagcac gctggttttg ccactgccgt
    40741 tggcacctac cagaaagtta aagccgggag ataaagcgag atccgcggtt tcaatattgc
    40801 ggaaatcgcg gatcaacaag cgggtgaggg acattacagt ctcattggca tgacaacata
    40861 ggcagccgac tgtgatgcgg catcttcaat ctgcacgctg gaaaccgaat cggtcagcat
    40921 catgcggacg ttttcgcatt tcagcgcgtt cagaacatcc agcacatagc tgacgttgaa
    40981 gccgatttcc atctccgcac cgctataggt aacgtcgagg atctcttccg cttcttcctg
    41041 ttccgggttg ttggcagtaa ttttcagctg gttttcgctg acatacaggc gcacaccgcg
    41101 gaatttctcg ttagagagaa tcgccgcgcg agcaaacgcc tgcttgagca gatcgcagcc
    41161 tgcttccaga tgtttgtccg ggttcttcgg cagaacgcga cggtaatccg ggaagcgacc
    41221 atccaccagt ttcgaggtga aaataaagtc gccaacgtgg gcacgaatgt tattgctgcc
    41281 aatctgcaca cgcagcagat tgtcgccgcc gtcgagcata cgcatcagtt caatcacgcc
    41341 tttacgcggt acgatcaccg aatggcttgg caaagattga ccaattggca ttgaacagac
    41401 cgccagacgg tggccgtcgg ttgccacggt gcgcagttct tcaccttcgg tttcaaacag
    41461 cataccgttt aagtaatagc gaacatcctg atgcgccatc gaaaactggg ttgcttcaat
    41521 cagacgcttc atcgtcgcct gcggcagggt aaattcgact tcgctctgcc agtcatcgag
    41581 gttcgggaaa tccgccgctg gcagggtaga cagcgaaaaa cggctacgcc cggagcgcac
    41641 cagcatccgt tcaccttcca gctgcacggc aatttccgcg ccttcaggca gaccacggca
    41701 gatatcaaag aatttgcgcg ccggaacggt cgtcgctcct ggctcgtgtg gctgaaccag
    41761 cgcaacgcgt gccaccattt ccatctcgag atcagtaccg gtcagcgaca acgtaccgtc
    41821 agcaacctgt aacagcagat taccgagaat cggtagcgta ggacgaccac ctaacggacc
    41881 gctcacctgt tgtagcggtt ttaataaatg ctcacgttct acggtaaatt tcataggttt
    41941 acgatgacaa tgttctgatt aaatttgaaa aatcttcttt gatatcgtgg ctctcttcac
    42001 gcaactgctc gatcttacgg caggcatgaa gcaccgtcgt gtggtcacgc ccaccaaacg
    42061 catcgccaat ctctggcaga ctgtggttag tcagctcttt cgctagcgcc atcgccatct
    42121 ggcgcggacg cgccaccgag cgggatcgac gcttggaaag gagatccgcg actttgatct
    42181 tgtagtactc cgccaccgtc ttctgaatat tgtcgatggt gaccagtttt tcctgcaatg
    42241 ccagcaagtc gcgcagcgcc tcacgcacga agtcgatggt gatcgcccgt ccggtaaagt
    42301 tggcgttagc aataacgcgg ttcagcgccc cttccagctc acgtacgtta gatcgtagac
    42361 gcttggcgat aaagaacgcc acttcacccg gcaaacgaat gtcattttcg tcggcctttt
    42421 tcatcaggat cgccacacgg gtttccagct ctggcggttc gatcgccaca gtcagtcccc
    42481 aaccgaagcg ggatttcaaa cgatcctcaa cgccgttgat ctctttcgga tagcgatccg
    42541 aggtgagaat gatctgttga ttaccttcca gcagggcgtt gaaggtgtgg aaaaactctt
    42601 cctgagatcg ttctttatta gcaaaaaact gaatatcgtc gatcagcagt gcatctacgg
    42661 aacggtagta gcgtttaaac tcttcgatcg cgttgttttg cagggcttta accatgtcct
    42721 gaacaaagcg ctcggagtgc atataaacca ctttggcatt cggcttgcgc gccataatgc
    42781 cgttacccac cgcatgcagc aggtgagttt tacccagacc cgtgccgcca taaaggaaca
    42841 acgggttata ggcaccgcca gggttatccg ccacctggca agccgccgcg cgcgccagtt
    42901 ggttagattt accttcaacg aagttatcaa acgtgtgttt gacgtttacg ttagaacgat
    42961 aggtcggttc tgccggggcc gggacgttat cccaacctga gcgcgtagaa ggcgcagcac
    43021 gttgcggctg cgtttgcgcc acctgtgcag gggccgcgac gttgctcgtc actgccgctt
    43081 gtggcgtttg cgtcaccggt ttggtgccga cttcaaaacg cagctgtggg gcatccgctc
    43141 cgcagaaact ggttagcagt ccattgatat tattaaggta cttgtcccgt acccaatcga
    43201 ggacaaaacg gtttggcgcg tacagggcca gcgtgttatc gctcagttcc gcctgcaatg
    43261 ggcgtatcca catactgaat tctgtggctg gtaactcatc ctgcaatcgg gcaagacact
    43321 gctgccaaag cgaaagtgac acggcggact ccactcgaac aaaagtcgat aatgactaag
    43381 gctgaaacat tcatgattgt tgacgtacgt cgaaaagacc ctgcttgagg gtgacgcacg
    43441 aaccgctgtc tgcggttata tgcccgatca agatcctgca aaacgatcgg gaccgcggat
    43501 catagcctaa actgcgcaag agatcttctg tttctcacag attttcccta tttatccaca
    43561 ggactttcca gaactcgcta agtgtaaacg atcctgccgc gaggcgggca cgatttacgc
    43621 cgcatattgg aaaatttaat gaccatagac aaaaattggc ttaatcgatc taataaagat
    43681 ccaggacgat ccttgcgctt tacccatcag cccgtataat cctccacccg gcgcgccatg
    43741 ctggtttcca ctggtgtgag gtcgtacatt ttccctgcga aaaggtgcgg aaaagcgcgg
    43801 taaataagga aagagaattg actccggtgt gtacaattat tacaatccgg cctctttaat
    43861 cacccatggc ttcggtgtcc atcgtttcat ttttcggcgg atatccaata aagccattga
    43921 atttattcaa gtttaggtag aaatcgccat gaaacgcact tttcaaccgt ctgtactgaa
    43981 gcgcaaccgt tctcacggct tccgtgctcg tatggctact aaaaatggtc gtcaggttct
    44041 ggcacgtcgt cgtgctaaag gccgcgctcg tctgaccgtt tctaagtaat aaagctaacc
    44101 cctgagtggt taagctcgca tttcccaggg agttacgctt gttaactccc agtcaattca
    44161 cattcgtctt ccagcagcca caacgggctg gcacgccgca aattaccatt ctcggccgcc
    44221 tgaattcgct ggggcatccc cgtatcggtc ttacagtcgc caagaaaaac gttcgacgcg
    44281 cccatgaacg caatcggatt aaacgcctga cgcgtgaaag cttccgtctg cgccaacatg
    44341 aactcccggc tatggatttc gtggtggtgg cgaaaaaagg ggttgccgac ctcgataacc
    44401 gtgctctctc ggaagcgttg gaaaaattat ggcgccgcca ctgtcgcctg gctcgcgggt
    44461 cctgatagcc ctcattcggg tctatcaacg cctgattagt ccgctactcg ggccgcattg
    44521 tcgtttcact ccaacctgtt caagctacgg aattgaggca ttgcgcaggt ttggagtgat
    44581 aaaaggcagt tggttgacgg tgaaacgcgt attaaaatgc caccctttac accctggtgg
    44641 tgacgatccc gtcccgcccg gaccatttga taccagagaa cactaacgat ggattcgcaa
    44701 cgcaatcttt tagtcatcgc tttgctgttc gtgtctttca tgatctggca agcctgggag
    44761 caggataaaa acccgcaacc tcaggcccaa cagaccacgc agacaacgac caccgcagcg
    44821 ggtagcgccg ccgaccaggg cgtaccggcc agtggccagg ggaaactgat ctcggttaag
    44881 accgacgtgc ttgatctgac catcaacacc cgtggtggtg atgttgagca agctctgctg
    44941 cctgcttacc cgaaagagct gaactctacc cagccgttcc agctgttgga aacttcaccg
    45001 cagtttattt atcaggcaca gagcggtctg accggtcgtg atggcccgga taacccggct
    45061 aacggcccgc gtccgctgta taacgttgaa aaagacgctt atgtgctggc tgaaggtcaa
    45121 aacgaactgc aggtgccgat gacgtatacc gacgcggcag gcaacacgtt taccaaaacg
    45181 tttgtcctga aacgtggtga ttacgctgtc aacgtcaact acaacgtgca gaacgctggc
    45241 gagaaaccgc tggaaatctc ctcgtttggt cagttgaagc aatccatcac tctgccaccg
    45301 catctcgata ccggaagcag caacttcgca ctgcacacct tccgtggcgc ggcgtactcc
    45361 acgcctgacg agaagtatga gaaatacaag ttcgatacca ttgccgataa cgaaaacctg
    45421 aacatctctt cgaaaggtgg ttgggtggcg atgctgcaac agtatttcgc gacggcgtgg
    45481 atcccgcata acgacggtac caacaacttc tataccgcta atctgggtaa cggcatcgtc
    45541 gctatcggct ataaatctca gccggtactg gttcagcctg gtcagactgg cgcgatgaac
    45601 agcaccctgt gggttggccc ggaaatccag gacaaaatgg cagctgttgc tccgcacctg
    45661 gatctgaccg ttgattacgg ttggttgtgg ttcatctctc agccgctgtt caaactgctg
    45721 aaatggatcc atagctttgt gggtaactgg ggcttctcca ttatcatcat cacctttatc
    45781 gttcgtggca tcatgtaccc gctgaccaaa gcgcagtaca cctccatggc gaagatgcgt
    45841 atgctgcagc cgaagattca ggcaatgcgt gagcgtctgg gcgatgacaa acagcgtatc
    45901 agtcaggaaa tgatggcgct gtacaaagct gagaaggtta acccgctggg cggctgcttc
    45961 ccgctgctga tccagatgcc aatcttcctg gcgttgtact acatgctgat gggttccgtt
    46021 gaactgcgtc aggcaccgtt tgcactgtgg atccacgacc tgtcggcaca ggacccgtac
    46081 tacatcctgc cgatcctgat gggcgtaacg atgttcttca ttcagaagat gtcgccgacc
    46141 actgtgaccg acccgatgca gcagaagatc atgaccttta tgccggtcat cttcaccgtg
    46201 ttcttcctgt ggttcccgtc aggtctggta ctgtactata tcgtcagcaa cctggtaacc
    46261 attattcagc agcagctgat ttaccgtggt ctggaaaaac gtggcctgca tagccgcgag
    46321 aagaaaaaat cctgattcgg tgagttttcg ctaaaataag ggcggtcagt tgaccgcctt
    46381 ttttcttttc gtagggcgga taagcaccgc gcatccgcca cacaaagcaa caggaacatc
    46441 atgagcgata atgacactat cgtagcccag gccacgcctc cgggacgtgg cggcgttggc
    46501 atcctgcgca tctccggctt caaagcccgt gaagttgccg aaaccgtgct gggtaaactg
    46561 cctaagccgc gctacgccga ttatcttccg tttaaagacg ccgacggcag cgtgctcgat
    46621 caggggattg cgctatggtt ccctggcccg aactcgttca ccggcgaaga tgtgctggaa
    46681 ctgcaaggtc atggcggtcc ggtgatcctc gacctgctgt taaaacgcat tctgaccatt
    46741 cccggcctgc ggattgctcg ccctggtgag ttttccgaac gcgcgtttct taacgataaa
    46801 cttgacttag cccaggccga ggcgattgcc gatcttatcg acgccagttc ggaacaggcg
    46861 gcccgttcgg cacttaactc gctgcaaggc gcattctccg cacgggttaa tcatctggta
    46921 gaagccctca cccacttgcg catttacgtc gaagcggcaa ttgatttccc tgatgaagag
    46981 atcgatttcc tctccgacgg caaaattgaa gcccagctta atgacgtgat ggccgatctc
    47041 gatgcagtgc gtgctgaagc acgtcagggt agtttgttgc gcgaagggat gaaagtagtg
    47101 attgccggac gtcctaatgc cggtaaatcg agcctgttaa acgcgctggc gggccgtgaa
    47161 gcggcaatcg taaccgatat cgccggaacc acgcgtgacg tgctgcgtga gcatatccac
    47221 attgacggaa tgccgctgca tatcatcgac actgccgggc tacgtgaggc cagtgacgaa
    47281 gtggaacgta ttggtatcga gcgcgcgtgg caggaaattg aacaggccga ccgcgtgctg
    47341 tttatggtcg atggcaccac gacagacgcc gttgatccgg cagagatctg gccggaattt
    47401 atcgcccgtc tgcccgcgaa actgccgatc accgtggtac gcaataaagc cgatatcacc
    47461 ggcgaaacgc tgggaatgag tgaagtgaac ggtcacgcgt taattcgtct ctcggcaagg
    47521 actggtgaag gcgtggacgt gctgcgtaac catctcaaac agagcatggg ctttgacacc
    47581 aatatggaag gcggcttcct ggcgcgccgt cgccacttac aggcgctgga acaggcagcg
    47641 gagcatttgc agcaaggtaa agcgcaactg ttgggagcct gggcaggtga actgctggcg
    47701 gaagagttgc gcctggcaca gcagaactta agcgaaatca ccggggaatt tacttcagac
    47761 gacctgctgg ggcggatttt ctccagcttc tgtattggta agtaaccgcg cttacgaagc
    47821 cgtattctga ctgtcagatg cggcttcgct tcattgttag cactcctgtt atgcctcaac
    47881 ccttttttaa acattaaaat tcttacgtaa tttataatct ttaaaaaaag catttaatac
    47941 tgctccccga acgattgtga ttcgattcac atttaaacaa tttcagaata gacaaaaatt
//
LOCUS       DAJTYL010000015        47294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYL010000015 DAJTYL010000000
VERSION     DAJTYL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.015"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO572_000867"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO572_000867"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6659962.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO572_000868"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO572_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6659963.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO572_000869"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO572_000869"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6659964.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO572_000870"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO572_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6659965.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO572_000871"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO572_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6659966.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO572_000872"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO572_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6659967.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO572_000873"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO572_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6659968.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO572_000874"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO572_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6659969.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO572_000875"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO572_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6659970.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO572_000876"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO572_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6659971.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO572_000877"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO572_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6659972.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO572_000878"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO572_000878"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6659973.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO572_000879"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO572_000879"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6659974.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO572_000880"
     CDS             11231..12085
                     /locus_tag="OO572_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6659975.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO572_000881"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO572_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6659976.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO572_000882"
     CDS             complement(13548..14687)
                     /locus_tag="OO572_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6659977.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO572_000883"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO572_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6659978.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO572_000884"
     CDS             15873..17234
                     /locus_tag="OO572_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6659979.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO572_000885"
     CDS             17231..17749
                     /locus_tag="OO572_000885"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6659980.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO572_000886"
     CDS             17749..18069
                     /locus_tag="OO572_000886"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6659981.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO572_000887"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO572_000887"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6659982.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20110
                     /locus_tag="OO572_000888"
                     /pseudo
     CDS             18888..20110
                     /locus_tag="OO572_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20207..20629
                     /locus_tag="OO572_000889"
     CDS             20207..20629
                     /locus_tag="OO572_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6659983.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20677..21549)
                     /locus_tag="OO572_000890"
     CDS             complement(20677..21549)
                     /locus_tag="OO572_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6659984.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21561..22655)
                     /locus_tag="OO572_000891"
     CDS             complement(21561..22655)
                     /locus_tag="OO572_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6659985.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22688..23686)
                     /locus_tag="OO572_000892"
     CDS             complement(22688..23686)
                     /locus_tag="OO572_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6659986.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23711..25222)
                     /locus_tag="OO572_000893"
     CDS             complement(23711..25222)
                     /locus_tag="OO572_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6659987.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25245..26227)
                     /locus_tag="OO572_000894"
                     /pseudo
     CDS             complement(25245..26227)
                     /locus_tag="OO572_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26324..29425)
                     /locus_tag="OO572_000895"
     CDS             complement(26324..29425)
                     /locus_tag="OO572_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6659988.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29432..29605)
                     /locus_tag="OO572_000896"
     CDS             complement(29432..29605)
                     /locus_tag="OO572_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6659989.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29723..30916
                     /locus_tag="OO572_000897"
     CDS             29723..30916
                     /locus_tag="OO572_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6659990.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31114..32367)
                     /gene="glyA"
                     /locus_tag="OO572_000898"
     CDS             complement(31114..32367)
                     /gene="glyA"
                     /locus_tag="OO572_000898"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6659991.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32694..33884
                     /gene="hmpA"
                     /locus_tag="OO572_000899"
     CDS             32694..33884
                     /gene="hmpA"
                     /locus_tag="OO572_000899"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6659992.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33929..34267)
                     /gene="glnB"
                     /locus_tag="OO572_000900"
     CDS             complement(33929..34267)
                     /gene="glnB"
                     /locus_tag="OO572_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6659993.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34328..35662)
                     /gene="glrR"
                     /locus_tag="OO572_000901"
     CDS             complement(34328..35662)
                     /gene="glrR"
                     /locus_tag="OO572_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6659994.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35652..36365)
                     /gene="qseG"
                     /locus_tag="OO572_000902"
     CDS             complement(35652..36365)
                     /gene="qseG"
                     /locus_tag="OO572_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6659995.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36530..37957)
                     /gene="qseE"
                     /locus_tag="OO572_000903"
     CDS             complement(36530..37957)
                     /gene="qseE"
                     /locus_tag="OO572_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6659996.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38515..42402)
                     /gene="purL"
                     /locus_tag="OO572_000904"
                     /gene_synonym="purI"
     CDS             complement(38515..42402)
                     /gene="purL"
                     /locus_tag="OO572_000904"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6659997.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42661..44217
                     /gene="mltF"
                     /locus_tag="OO572_000905"
     CDS             42661..44217
                     /gene="mltF"
                     /locus_tag="OO572_000905"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6659998.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44214..44750)
                     /gene="tadA"
                     /locus_tag="OO572_000906"
     CDS             complement(44214..44750)
                     /gene="tadA"
                     /locus_tag="OO572_000906"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6659999.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44775..45410)
                     /gene="yfhb"
                     /locus_tag="OO572_000907"
     CDS             complement(44775..45410)
                     /gene="yfhb"
                     /locus_tag="OO572_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6660000.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45619..46467
                     /locus_tag="OO572_000908"
     CDS             45619..46467
                     /locus_tag="OO572_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6660001.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46806..47294)
                     /locus_tag="OO572_000909"
                     /pseudo
     CDS             complement(46806..47294)
                     /locus_tag="OO572_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11274 a        12960 c        11897 g        11163 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac
    19441 cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccatcagca
    19501 ggctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt
    19561 agaactgacg ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg
    19621 tatcagcgcc aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt
    19681 cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat
    19741 cactcgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca
    19801 ggcgcaaatt gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg
    19861 gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga
    19921 ctggctttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg
    19981 cgtgcttatc ggtgcggtca cgctgaatca gggccgtgag attcgcccaa ttcgcaaatg
    20041 gatccagagc ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa
    20101 atcactgtaa tcaggataat tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag
    20161 agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg
    20221 ttattttgat tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt
    20281 cttaattttc attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata
    20341 tatggcctcg ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga
    20401 agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt
    20461 tattttctac acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga
    20521 tgcggttggg ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt
    20581 gctattggca attaccggac cgggggcaat ttctctcgat cggcgttagg aaagatgccg
    20641 gatgcggcgt gaacgcctta tccggcattt tataaattac agccactcta cccgcaacga
    20701 catctctgag gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg
    20761 atgatgctca tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc
    20821 tttatcaaac gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc
    20881 catgatgatg gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc
    20941 aaaaccattg ttcacccact gacgcggcaa cggcgcgagc tggttaaaat ccagttcctg
    21001 cggtagctgc tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct
    21061 cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc
    21121 aaatggcagc gtctctgccc cttgattggt gacagagagc gtcaccgtca gcgtatccgc
    21181 cgttaaatga aacgcctgac ttacccgata gtgatagaca ccgctgcgat gttcatacac
    21241 cagacacaaa ctatcatcgc tgtgcgagac acattgccac tcgcccagcc agccatcgcc
    21301 gtgtagatag tgcgcatccc actcaacatt cggttgcagt tgatactcac gcccctgcca
    21361 gacaaaacgg ttgccgctca cccggtttgc aaatggcacc agcggaaaac atgaggcatc
    21421 ggttgccaca ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc
    21481 ttcgattacg ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata
    21541 gatggtcatc cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg
    21601 ctcgccatca gcacataggc atcacctgcc tgttctagcg agaagcgatg ggtaatggcg
    21661 ttacgcggcc acagcttcca gtcagtcaaa tcgtgggcgc atttttccat atggaacaga
    21721 ctggtcaccc aggagccgat aatccgccgt tgatggtgca tcagatcagc gctgacctcg
    21781 aattccactt ttccggtttc accaatgtaa accacccgcc cccagtcagc agtggattgc
    21841 agtgccagca agcgacctgc ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg
    21901 tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc
    21961 atccccccta actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc
    22021 cgttttgcac cgcgaccttt cgccagcatc atcgccatca tgccgaccgg ccccagaccg
    22081 accaccagca cgttatcgct gccggaaact tcgccgcgca aaatcccttc ataggccgtg
    22141 ccaacgccgc aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc
    22201 agatcttttt cttccgccag cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa
    22261 gccgcttttc ctttgccagt acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa
    22321 ccacagccag aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct
    22381 tgccccatcg ccacaatctg cccgcacggt tcatgaccgt tgataaatcc ctggtataac
    22441 ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt
    22501 ccgcaaatcc cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc
    22561 gcaacttccc gcagatcgac ggttgaattt cctagtaaat aagcagccag catcgttttc
    22621 ataaatccct cattaacaat acgattaatt ttcatccctg cagcacccgc gccaggggcg
    22681 ttaaggttta gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg
    22741 ccaccacgat gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca
    22801 ccagaccgtt accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac
    22861 cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca
    22921 gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc cgccaacaaa atgccggtca
    22981 ccgccgctaa taatcccgaa agggtaaaga taagaatgcg cactcgacga acgttaatac
    23041 cgcacaactg cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg
    23101 tttttcggct aatgaacaca aacagcgcaa acaacaccat catgatcagc gcggatactg
    23161 gcacaccgag aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg
    23221 gcagtgcgtt ggtcataaac agccccattc cgcgcagagc gctccacagc cccagtgtgg
    23281 caacgaaact tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg
    23341 cgcctaacag caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa
    23401 atgccaggca caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg
    23461 agataataat cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca
    23521 cgttcatctg gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa
    23581 gaatggcaat caccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga
    23641 gcgagacgct cttgccctgc ggcaatggta atgacgaagc atacatcttc aggttcctcg
    23701 gtaaaattca tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg
    23761 agtgaaactc ctgcgagaac gtgccgtgct gtaataacag gatgcggtca cacaccagcg
    23821 gcaattcctc cacttcactg gagataaaca ccacgctttt tccttcggca gccagctcac
    23881 ggacaatacg gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga
    23941 gcaacaaaat ctggctggcg gcatagaccc aacgaccgat caccactttt tgctgattgc
    24001 caccagaaag cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc atccgctgca
    24061 tcacctcttc ggtcaggcgg cggatggtgg accattgcag cacaccgttg gtgctgattt
    24121 tttgccgatt ggtcagcact gtattttcgt caacgcctag ccagggaatg atccccgctt
    24181 ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag catgtcgccg taatcggggc
    24241 gcatgatttt ctcgccgttg ataacaattt cgccctgttc atacgtctcc agcccaacaa
    24301 tcgccttcag caattcactg cgccctgccc ccagcaggcc agcaatgcca agcacttcgc
    24361 cacgacgtag cgtaaagctg atatcctcca gcttgggctt atggcgtaac gcacggactt
    24421 ccagtacggc ctgatccaca atttcctgag gggcaaccgg cgcaatatca acgtgatcac
    24481 gccctagcat tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg
    24541 ccacctgacc atcgcgcata acggtggcat aggaggcaat gcggcggatt tcttccatcc
    24601 ggtggctgac ataaatcacc gccacgccca gtgccgacat ctttttcacc gcactgatca
    24661 ccagttcaac ttctgcactg gcaagcgagc tggtgggttc atcaagaatg accacgcgcg
    24721 gctcgccctt catcacccgt gcaatttcca ccagttgctt ttgcgccggg cttagcgttg
    24781 aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct
    24841 gcgccatttg caggtaatca atcatgccgt tgcggcgagg ccattgacca aggcagaggt
    24901 tttctgccac cgtcagccct tccaccagac ttaattcctg ataaaccgca cgaaccccca
    24961 gttcagcggc acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc
    25021 agatatcacc gctatccggg cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc
    25081 ccgcgccgtt tttacctaac agcgcacgaa cttcgccttt attgagcgtg aagttaacgt
    25141 tatccagcgc aacgacgccg gggtaacgct tatttcctgc caccactttt gctaccggga
    25201 ctgcctctgt tgccgtgaac atagggacct ctgcgaatca gcgattaggg cagaccatca
    25261 acgtgcgttg ccagccactg tttaccatct tccgttttgg tatagagatc gattggcacc
    25321 tgaatcactt tttcaccgtc ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca
    25381 gcattgccca ttttcttacc ggaaatatcc actaccgctt tcagcacctg attgttttcc
    25441 agctcctgag caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga
    25501 ttttgattac gtaccgcttt taccgcgccg agtgtcgcac cgcccgactc ccccataatg
    25561 gcgttgagat ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc tttatctaaa
    25621 acagtccctt cctggttagc gacaatttgc gcgccgggaa cgcgggattt taatacttct
    25681 tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt cgcaattgat gacggcaatt
    25741 ttcggctggt caattttatt ggcaataaaa taatcggcgg cagcgttacc cagtttttac
    25801 caaattccag tggatcgccg accagatacg ccgagacata tttatcgaca cccttttgat
    25861 taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac
    25921 ggctactgcc attttcagac actgccgata aaataatggc atcgacatta cgcgccacga
    25981 gggtatcaac aaaggtactt tctttcgaaa tatccccctg ggcgttagtt tcaattaact
    26041 gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat
    26101 atccctgggt atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa
    26161 atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt
    26221 taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga
    26281 tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa
    26341 aaataaaagg catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg
    26401 tcaattcagc gcgagcagac tcatattgcg caacctcccc gagtcccgcg gacgccagca
    26461 tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac agcgataaca
    26521 ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg
    26581 cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca
    26641 gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt
    26701 taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc
    26761 cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta
    26821 aacggccttc gcttaaattc tccggataat acagcgcggc atgaagcagt tcgctggcct
    26881 gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact
    26941 gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt
    27001 ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt
    27061 cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg
    27121 tggctccact taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg
    27181 caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt ttcttattcc
    27241 acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac
    27301 gttgccataa ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag caggccagta
    27361 aatggcgagc aaaccaacac tcttcaatac tctccagcgc cgccacttct tccagcgtat
    27421 tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt
    27481 cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg
    27541 cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca
    27601 tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc
    27661 tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca
    27721 gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat
    27781 gcaagcaaag cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca
    27841 gacccgcgtc gaagttatca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg
    27901 ctttactatt gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc
    27961 cctgacgttc gtaagcactg gcgcgaatca aacttgcttg tccacactgc ggttttttgt
    28021 tcagcgcatg tgcacgtttc agcgcctgac tggcatacgc caccgcttgc gggaaatctg
    28081 cgcggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc gggtccagcg
    28141 ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat
    28201 gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt
    28261 tggcgacgtc cggcaacggc aactcctgcg gctgatgttc ctgataactc agtacaatat
    28321 tgccgtcggc atcggagagt tcaatggtca gtctgtcagg attgataccg tgcaacacgc
    28381 cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc
    28441 cgatttcgcg gatcgccaga cggtatccgt tcaacggaga gatggcatac agcccccact
    28501 caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca
    28561 tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa
    28621 gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc
    28681 cgttattgtc ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc
    28741 actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat
    28801 cgtgacacca cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa
    28861 caggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag
    28921 tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca
    28981 catccggtgg gaaaacgctc tgatgccctt cacccccttt cactgccggg ttgacccacc
    29041 acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagtgccg
    29101 cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
    29161 cgccgaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca
    29221 taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg
    29281 gtcccagcat ccccaccagc gcaggtttaa tgacttcatt gtgatagaca aaatcgcgct
    29341 gtttcacttt atcccatgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt
    29401 tgatgtagtc gttttccagc cacaccgcct gctaggattt cagggttttc tgctcgctca
    29461 gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt
    29521 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
    29581 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
    29641 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
    29701 aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca
    29761 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
    29821 ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
    29881 cgaaaaacgg gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac
    29941 atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat
    30001 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc gaatttgagt atttacagat
    30061 tgatgtaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
    30121 taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc
    30181 ggtgaccggc gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag ttgaggtgaa
    30241 gtatctgctg gaagagaagc tgggcattcg ggtgctggtc gataatgact gcgtgatgct
    30301 ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt
    30361 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta
    30421 tggtagcgga cagattggtc acaccatcgt taatccggat ggagtcgtct gcgactgtgg
    30481 acgttacggc agcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt
    30541 atggctaaaa tcacaaccgg ttaatactca gcttgagcct gaaaaactga caaccgcgca
    30601 gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc
    30661 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    30721 gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac
    30781 aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt
    30841 cgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca
    30901 gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga
    30961 tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata
    31021 tattgaattt gcaagatttt gtaggcctga gaaggtgttc atgccgcatc caacatgaac
    31081 aacgagcaca ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca
    31141 gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag
    31201 cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc cgcgacgggt
    31261 aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg
    31321 tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc
    31381 ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac
    31441 cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tcgcgacctg
    31501 ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc
    31561 gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt
    31621 gtacagctct tcgctaccac ctttcgccaa gatcaggccg ccgcgcggac ccgccagggt
    31681 tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc
    31741 agcaaccagg cccgcaacgt gcgccatatc aacgaacaga taagcaccga tgctgtcagc
    31801 gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat
    31861 cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga tatgaccggt
    31921 agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc
    31981 gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag
    32041 cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta
    32101 gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata
    32161 ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg
    32221 agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat
    32281 gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc
    32341 ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt
    32401 ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg
    32461 ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac gcaaggcttt
    32521 ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt
    32581 tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt
    32641 tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaggaagac catatgcttg
    32701 acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa
    32761 agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt
    32821 ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg
    32881 cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc
    32941 agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt
    33001 tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag
    33061 cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg
    33121 ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc
    33181 gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat
    33241 accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa
    33301 ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc gcggtgaaac
    33361 gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg
    33421 tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga
    33481 cgttaatctc taccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa
    33541 aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc gaagttcacg
    33601 cctttgctga tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct
    33661 ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga
    33721 tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg
    33781 gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg
    33841 aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat
    33901 ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac
    33961 ggatgcgaat gacccgagcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg
    34021 tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa
    34081 tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg
    34141 tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga
    34201 cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct
    34261 ttttcatgct attccttgaa aaggttgcct gtcttttgat ctgctaaacg taacacataa
    34321 cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa
    34381 attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt
    34441 gcagcagctt acgcaagtag ttgagttcaa aatgattacg tgcctcaaca aaggttggca
    34501 gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg
    34561 aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc
    34621 tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc
    34681 gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg
    34741 ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt
    34801 ccccgcgcgc catcgctttt gacagatcac ggtgagtggc agaaataatc cgcacattga
    34861 tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca
    34921 gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt
    34981 ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat
    35041 gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa
    35101 atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg
    35161 tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt
    35221 ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg
    35281 tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga
    35341 caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag
    35401 aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa
    35461 atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt
    35521 cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt
    35581 cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata
    35641 aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca
    35701 ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt
    35761 tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg
    35821 tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa
    35881 cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac
    35941 agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg
    36001 cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt
    36061 tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga
    36121 tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc
    36181 agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt
    36241 ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat
    36301 gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt
    36361 cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga
    36421 catggacagc gcggagaaac cggggcgtac atatcgtacg tgaagatttc gagcactacc
    36481 cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt
    36541 ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat
    36601 acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc
    36661 ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc
    36721 gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat
    36781 gtaaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt
    36841 aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga
    36901 gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg
    36961 tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac
    37021 ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg ctatcaagaa
    37081 tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctgatca gccagtaatt
    37141 cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa
    37201 attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc
    37261 caaccgagcg taactcgctc ggtccacaga acgaaacgct attgcccaga gaacgccctt
    37321 cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga
    37381 aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt
    37441 gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg
    37501 tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag
    37561 catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg
    37621 cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac
    37681 gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac
    37741 ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa
    37801 taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg
    37861 cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt
    37921 gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga
    37981 atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt
    38041 tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc
    38101 tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac
    38161 gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact
    38221 gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat
    38281 ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag
    38341 ataaacttat tagattgcaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt
    38401 aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga
    38461 tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc
    38521 caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc
    38581 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    38641 gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc
    38701 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    38761 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    38821 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt
    38881 tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    38941 ccacaactca ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    39001 tacccccagg gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg
    39061 gtcattgaac aggatagact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    39121 gaaacctccg cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc cagtcagcag
    39181 gtcgctcata tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg ccgccatttc
    39241 aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc
    39301 aatatacggt gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg
    39361 atcggcgtcg ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag
    39421 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct
    39481 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac
    39541 ataatggacg caatccgcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    39601 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc
    39661 gtcacccaga gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg ccatttccgc
    39721 cagcgttacc agcaggcctc catcagagcg gtcgtgatac gccagcagct tacgctgtgc
    39781 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgg acaacatcgc gtacatctgc
    39841 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    39901 gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtacaaagct gcggcgtgat
    39961 ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    40021 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    40081 caccgggatc gtcaggccca gcgccggaca aagttcttca cccacggctt taacggcttc
    40141 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    40201 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    40261 cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat
    40321 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    40381 ctgccacggc cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat
    40441 ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    40501 tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    40561 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    40621 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    40681 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    40741 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    40801 ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt cgctcaccag
    40861 ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca gaattgggtt
    40921 agcatcacca agctgccagc aacggtcgat cacttcctgg caacgacgct ccatctccgg
    40981 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga
    41041 agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    41101 cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    41161 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt
    41221 ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc
    41281 cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggctt
    41341 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa
    41401 accaaccaaa ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc
    41461 accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    41521 ttcgactttc atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc ccgtttcgtg
    41581 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    41641 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt
    41701 cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc
    41761 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    41821 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaagccaa gacgcaggtt
    41881 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg
    41941 ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat
    42001 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    42061 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    42121 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    42181 gagtttgcct tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag
    42241 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    42301 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    42361 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    42421 gctttcggtg tacccaaggg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    42481 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    42541 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    42601 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    42661 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    42721 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    42781 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    42841 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    42901 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    42961 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    43021 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    43081 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggta
    43141 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    43201 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    43261 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    43321 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    43381 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    43441 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    43501 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    43561 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    43621 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    43681 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    43741 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    43801 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    43861 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    43921 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    43981 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    44041 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    44101 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    44161 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    44221 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    44281 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    44341 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    44401 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    44461 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    44521 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    44581 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    44641 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccaata ttcgtggcta
    44701 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    44761 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    44821 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    44881 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    44941 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    45001 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    45061 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    45121 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    45181 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    45241 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    45301 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    45361 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    45421 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    45481 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    45541 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    45601 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaagggctt
    45661 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    45721 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    45781 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    45841 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    45901 ttacggctgg tcggcgaaaa actgattaaa gaaaataccg ccgctatgta cgcaacgcta
    45961 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    46021 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    46081 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    46141 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    46201 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    46261 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    46321 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    46381 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    46441 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    46501 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    46561 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    46621 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    46681 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    46741 gtgacataca acattaactt ttcagtcaac cgtagcacgt tcttgtatac gccgtgtctg
    46801 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    46861 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    46921 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    46981 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    47041 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    47101 aagttgatgc ctcgttcgcg caacgcctcc accagcacga caagatgccg catactgcgc
    47161 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cttgagcagt
    47221 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    47281 catcctgcac agtt
//
LOCUS       DAJTYL010000016        46765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYL010000016 DAJTYL010000000
VERSION     DAJTYL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.016"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..298)
                     /locus_tag="OO572_000910"
     CDS             complement(<1..298)
                     /locus_tag="OO572_000910"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6660002.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYE"
     gene            903..1316
                     /gene="hns"
                     /locus_tag="OO572_000911"
     CDS             903..1316
                     /gene="hns"
                     /locus_tag="OO572_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6660003.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1460..2368)
                     /gene="galU"
                     /locus_tag="OO572_000912"
     CDS             complement(1460..2368)
                     /gene="galU"
                     /locus_tag="OO572_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6660004.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2570..3583)
                     /gene="rssB"
                     /locus_tag="OO572_000913"
     CDS             complement(2570..3583)
                     /gene="rssB"
                     /locus_tag="OO572_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6660005.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3675..4580)
                     /gene="rssA"
                     /locus_tag="OO572_000914"
     CDS             complement(3675..4580)
                     /gene="rssA"
                     /locus_tag="OO572_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6660006.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4693..5151
                     /locus_tag="OO572_000915"
     CDS             4693..5151
                     /locus_tag="OO572_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6660007.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5201..6043
                     /gene="purU"
                     /locus_tag="OO572_000916"
     CDS             5201..6043
                     /gene="purU"
                     /locus_tag="OO572_000916"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6660008.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6203..6287
                     /locus_tag="OO572_000917"
     tRNA            6203..6287
                     /locus_tag="OO572_000917"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6237..6239,aa:Tyr,seq:gta)
     gene            6334..6465
                     /locus_tag="OO572_000918"
     ncRNA           6334..6465
                     /ncRNA_class="other"
                     /locus_tag="OO572_000918"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6649..7326)
                     /gene="narI"
                     /locus_tag="OO572_000919"
     CDS             complement(6649..7326)
                     /gene="narI"
                     /locus_tag="OO572_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6660009.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7326..8036)
                     /gene="narJ"
                     /locus_tag="OO572_000920"
     CDS             complement(7326..8036)
                     /gene="narJ"
                     /locus_tag="OO572_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6660010.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8033..9571)
                     /gene="narH"
                     /locus_tag="OO572_000921"
     CDS             complement(8033..9571)
                     /gene="narH"
                     /locus_tag="OO572_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6660011.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9568..13311)
                     /locus_tag="OO572_000922"
     CDS             complement(9568..13311)
                     /locus_tag="OO572_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6660012.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13704..15095)
                     /gene="narK"
                     /locus_tag="OO572_000923"
     CDS             complement(13704..15095)
                     /gene="narK"
                     /locus_tag="OO572_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6660013.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15434..17230
                     /gene="narX"
                     /locus_tag="OO572_000924"
     CDS             15434..17230
                     /gene="narX"
                     /locus_tag="OO572_000924"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6660014.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17223..17873
                     /gene="narL"
                     /locus_tag="OO572_000925"
     CDS             17223..17873
                     /gene="narL"
                     /locus_tag="OO572_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6660015.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(17874..19268)
                     /gene="ychO"
                     /locus_tag="OO572_000926"
     CDS             complement(17874..19268)
                     /gene="ychO"
                     /locus_tag="OO572_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6660016.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19453..19806
                     /gene="ychN"
                     /locus_tag="OO572_000927"
     CDS             19453..19806
                     /gene="ychN"
                     /locus_tag="OO572_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6660017.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(19850..20545)
                     /gene="chaC"
                     /locus_tag="OO572_000928"
     CDS             complement(19850..20545)
                     /gene="chaC"
                     /locus_tag="OO572_000928"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6660018.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20703..20933)
                     /gene="chaB"
                     /locus_tag="OO572_000929"
     CDS             complement(20703..20933)
                     /gene="chaB"
                     /locus_tag="OO572_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6660019.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21203..22303
                     /gene="chaA"
                     /locus_tag="OO572_000930"
     CDS             21203..22303
                     /gene="chaA"
                     /locus_tag="OO572_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6660020.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            22708..22815
                     /locus_tag="OO572_000931"
     CDS             22708..22815
                     /locus_tag="OO572_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6660021.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(22964..23818)
                     /gene="kdsA"
                     /locus_tag="OO572_000932"
     CDS             complement(22964..23818)
                     /gene="kdsA"
                     /locus_tag="OO572_000932"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6660022.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(23854..24663)
                     /locus_tag="OO572_000933"
     CDS             complement(23854..24663)
                     /locus_tag="OO572_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6660023.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(24667..25059)
                     /locus_tag="OO572_000934"
     CDS             complement(24667..25059)
                     /locus_tag="OO572_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6660024.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25056..25889)
                     /gene="prmC"
                     /locus_tag="OO572_000935"
     CDS             complement(25056..25889)
                     /gene="prmC"
                     /locus_tag="OO572_000935"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6660025.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(25889..26971)
                     /gene="prfA"
                     /locus_tag="OO572_000936"
     CDS             complement(25889..26971)
                     /gene="prfA"
                     /locus_tag="OO572_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6660026.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27013..28269)
                     /gene="hemA"
                     /locus_tag="OO572_000937"
     CDS             complement(27013..28269)
                     /gene="hemA"
                     /locus_tag="OO572_000937"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6660027.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            28483..29106
                     /gene="lolB"
                     /locus_tag="OO572_000938"
     CDS             28483..29106
                     /gene="lolB"
                     /locus_tag="OO572_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6660028.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29106..29957
                     /gene="ispE"
                     /locus_tag="OO572_000939"
     CDS             29106..29957
                     /gene="ispE"
                     /locus_tag="OO572_000939"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6660029.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30108..31055
                     /gene="prs"
                     /locus_tag="OO572_000940"
     CDS             30108..31055
                     /gene="prs"
                     /locus_tag="OO572_000940"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6660030.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31180..32859
                     /gene="dauA"
                     /locus_tag="OO572_000941"
     CDS             31180..32859
                     /gene="dauA"
                     /locus_tag="OO572_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6660031.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(32914..33192)
                     /gene="ychH"
                     /locus_tag="OO572_000942"
     CDS             complement(32914..33192)
                     /gene="ychH"
                     /locus_tag="OO572_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6660032.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            33470..34054
                     /gene="pth"
                     /locus_tag="OO572_000943"
     CDS             33470..34054
                     /gene="pth"
                     /locus_tag="OO572_000943"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6660033.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34171..35262
                     /gene="ychF"
                     /locus_tag="OO572_000944"
     CDS             34171..35262
                     /gene="ychF"
                     /locus_tag="OO572_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6660034.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36106..38973
                     /locus_tag="OO572_000945"
     CDS             36106..38973
                     /locus_tag="OO572_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6660035.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLSAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39073..40992)
                     /gene="dhaR"
                     /locus_tag="OO572_000946"
     CDS             complement(39073..40992)
                     /gene="dhaR"
                     /locus_tag="OO572_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6660036.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41220..42290
                     /gene="dhaK"
                     /locus_tag="OO572_000947"
     CDS             41220..42290
                     /gene="dhaK"
                     /locus_tag="OO572_000947"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6660037.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDAMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42301..42933
                     /gene="dhaL"
                     /locus_tag="OO572_000948"
     CDS             42301..42933
                     /gene="dhaL"
                     /locus_tag="OO572_000948"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6660038.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            42944..44362
                     /gene="dhaM"
                     /locus_tag="OO572_000949"
     CDS             42944..44362
                     /gene="dhaM"
                     /locus_tag="OO572_000949"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6660039.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            44682..46379
                     /gene="treA"
                     /locus_tag="OO572_000950"
     CDS             44682..46379
                     /gene="treA"
                     /locus_tag="OO572_000950"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6660040.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(46458..46678)
                     /locus_tag="OO572_000951"
                     /pseudo
     CDS             complement(46458..46678)
                     /locus_tag="OO572_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11698 a        12071 c        11490 g        11506 t
ORIGIN      
        1 attcatatac ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg
       61 cctgctgttc atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt
      121 ttgcaggcga tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa
      181 tttctgccgt atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca
      241 acaatgctgt agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca
      301 gccacaggcc ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt
      361 tttcagctca acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca
      421 taatccttct gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct
      481 ggtaactatt cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg
      541 agatagtgca atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct
      601 taatagggaa ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga
      661 taatctcccc ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc
      721 atcggtgtaa atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt
      781 attggcggca caaaataaag aacaattttg aattccttac attcctggct attgcacaac
      841 tgaatttaag gctctattat tacctcaaca aaccacccca atataagttt gagattacta
      901 caatgagcga agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag
      961 aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac
     1021 gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc
     1081 aatatcgcga aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg
     1141 ctgccgttaa atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg
     1201 ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca
     1261 aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt
     1321 ttgtagattg cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa
     1381 gacaatttaa taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg
     1441 gataacaccg atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa
     1501 attccgtgcc aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta
     1561 atttattacc gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt
     1621 cgatcagcat atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt
     1681 ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag
     1741 acggcgcaac atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg
     1801 gcgctaattc aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt
     1861 caaccatgat ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat
     1921 cctgtgacaa atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg
     1981 gttcatcacc cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac
     2041 cctgacgaac ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca
     2101 gttgacgttt tacacgtttt tccagcattg cttccagttc aaaactggta tcaaagtggt
     2161 tttcaataga gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa
     2221 tacattcatt cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg
     2281 ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt
     2341 ttttgacttt cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac
     2401 gtgttcatat ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga
     2461 aaaggaggcg agatagcata attaagacaa ttacttatac gaatggcgac gatagtacca
     2521 ccagccgaca ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag
     2581 acaacatcaa gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc
     2641 gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga
     2701 tttgcacctg gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga
     2761 gatttttcag ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg
     2821 cctgacgaag taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat
     2881 tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag
     2941 caagtacgcc attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt
     3001 ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacggt
     3061 aattaacccg gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt
     3121 tcgccgctgc ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt
     3181 cctcaacgcg cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc
     3241 gattcagatc tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg
     3301 caatatctgc catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt
     3361 tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca
     3421 tcaggtctgg agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta
     3481 ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat
     3541 cttcaacaat gagaatctgt tttccgacca atggctgcgt catgttctct cccctgactg
     3601 gctttactca atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta
     3661 gataaaaatt ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt
     3721 tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat
     3781 atttgcgggc aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt
     3841 ttaaggcggt tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct
     3901 gtcacagccc gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt
     3961 agagaatcac cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc
     4021 atcaaatgag catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc
     4081 gtaagagaaa taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt
     4141 gcaacgggtg ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg
     4201 ccttcagtaa accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga
     4261 cgggaacaat tttcgatctc tgtttccggc attatttcgc gatattgatt gaagacacgc
     4321 tcgccgcgca gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa
     4381 taactgaaag aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg
     4441 cccaccagcg aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt
     4501 agcgcattaa taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc
     4561 cctattttta tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca
     4621 tcgctactgc tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca
     4681 gggagattat ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt
     4741 tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg
     4801 cgttactgcg cttttgtgat gcaagacgct gattatttaa taaagacctg gcatccctct
     4861 tgtggagcag cggcgttacg tgccgaattg atgaccggat ttgcgcatac tgaatggttg
     4921 ggattgaccg tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt
     4981 gttgctcgat ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg
     5041 aaagaaaacg gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat
     5101 ccctgccctt gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg
     5161 gtacggttta gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa
     5221 agttctgcgt actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg
     5281 ctacaagcac gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg
     5341 cttttttatg cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct
     5401 cgatagcgca ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat
     5461 agtgattctg gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta
     5521 cggcggtctg gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct
     5581 ggttgagcgt tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga
     5641 gcacgatcaa aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc
     5701 gaagtatatg cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa
     5761 tattcaccat tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga
     5821 acgtggtgtg aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg
     5881 cccaatcatc atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat
     5941 gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc
     6001 gcaacgcgtc tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac
     6061 tggttacctt taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta
     6121 acactttaca gcggcgcgtc atttgatatg atgcgccccg cttcccgata agggagcagg
     6181 ctagtaaaaa gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa
     6241 atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag
     6301 ccccggaatt ctcaaacgaa tctgcaatca aatattctgc ccaagcgggg aaggatgaga
     6361 agcttcgacc aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc
     6421 gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttgaata
     6481 atcaaaacct gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca
     6541 atatcctccc ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc
     6601 ccataaaaaa accccgccga agcagggtct atgtgaacta aaattcgttt agtgacgagc
     6661 gcgcaccagc tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag
     6721 acgcgagaac gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat
     6781 aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc
     6841 ccagccaacc agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt
     6901 cagcaggccc agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt
     6961 agtggttgca cgcacgcgtg ggctgaacat acgacgtttc agcaaaagca cgccgccaat
     7021 cagacacagc acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat
     7081 cggcagccag gcttcataca tccagtgcgg cgtcaacata ccgaagaagt gaccgacaaa
     7141 aatccccaga ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag
     7201 catctggctg gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc
     7261 aatcaggaag accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa
     7321 ttgcattagt gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca
     7381 aagcgacgct gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta
     7441 acctgctctt cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg
     7501 gcaatttttt ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga
     7561 tcaaacatca cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga
     7621 atcggcgcga tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac
     7681 tccagataca gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc
     7741 tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca
     7801 tgttcaaata gcaacagtga cgtggcacgg ccacggtcga acagttcgct gtactggacc
     7861 tgggcatcga gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca
     7921 tcttctttcg acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc
     7981 cataaggcag catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat
     8041 gcggctccgt tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga
     8101 atttggtatc tgagccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg
     8161 ggaaggcttc ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt
     8221 tagcaatcgc cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt
     8281 ccagcgcacg ggtatcaact ttaccgtcaa cagtttcagc acgtttgtaa tgacgcatcg
     8341 ccagcatacg tttcagcgca cgcagtaccg gtttggtatc accggcggtc agcagattcg
     8401 ccagatactg taccggaata cgcaagcttt cgacgtctgg cagaattccg ttgctgccca
     8461 gctcgcccgc gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg
     8521 gcagtgtgcg atattctgga tgcagcggca gcgccagttt ccattccatc gccattttat
     8581 aaaccggcga ctgctgtgca gcttcaataa cgctcagcgg aataccgtct ttaatcgcct
     8641 gctcgatgac tttcggatcg ttcggatcga ggaacacgtc cagctgacgc tggtagagat
     8701 ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc
     8761 caagataacg gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa
     8821 tacgcggata gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt
     8881 ttttgtacgg gcatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca
     8941 ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg
     9001 ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact
     9061 ggctatacat cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttatcaaact
     9121 caccgcccag gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg
     9181 taatcagcga acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca
     9241 ggttctgata atcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt
     9301 tagcaaagat tttacccagc agcatggctc ggttacccat gcgcggttgc agtttgccat
     9361 tgattttacg gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc
     9421 cctggcccgg cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg
     9481 tccagacgtt tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca
     9541 gcaccatgcc gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc
     9601 ttcgccatct aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt
     9661 agaacctacg gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat
     9721 atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt
     9781 aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc
     9841 tgccggaaca cgctggctga caaccgcacg ggcagtcagt gcaccgttgc tgttgaagac
     9901 ttcaatccag tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac
     9961 caccggacca ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat
    10021 accccacttc tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt
    10081 ctggcctatc acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct
    10141 ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt
    10201 acgccatggg atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc
    10261 ttccagacca gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg
    10321 gaagcggatc ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa
    10381 ttcgctcagg gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagcgt
    10441 caggatcatc tctgccgcat caattgcggt gttcagcatc ggctggcctt ttgccggacc
    10501 ttccgctttg gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc
    10561 aatcccttta ccgccgttac cgattttctc catcagcggg ccgatagagg taaagcgttc
    10621 gtaagttgcc ggataatcgc gctctacgac cataatgtgc ggtgcagttt tgcctgggat
    10681 caggtcgcat tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc
    10741 agagtcgtgc tggataggca gcgtgacgat gtcggtttct ttacccagat agccgacgca
    10801 cacttcggag aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tcgcttccca
    10861 ggccggatcg accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat
    10921 gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct
    10981 cgacagacgg aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg
    11041 ccagtccact tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc
    11101 gtgctccgtc cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag
    11161 caggttagaa cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc
    11221 tgccgcaaaa cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat
    11281 cccggctttt tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga
    11341 cggcagccag cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg
    11401 ggatttgtcc gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg
    11461 gctggagtgg ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc
    11521 aagggcaaac gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata
    11581 gtgcgcccag ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag
    11641 accacgatag ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat
    11701 cgaacgaccg tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg
    11761 gctgcgagaa acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc
    11821 atagctggtt gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt
    11881 cagatcataa acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac
    11941 cggcagtttg tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc
    12001 gccaccaaag tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag
    12061 ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt
    12121 ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa
    12181 ggcgacagtt ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc
    12241 gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg
    12301 catgtcggtg tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa
    12361 ttcacgcagc attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg
    12421 tgccagccac agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt
    12481 tttggtgcct ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg
    12541 cacgtttgaa ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac
    12601 gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta
    12661 gaagcttaag caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac
    12721 catcgacatt gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat
    12781 ggtgtaaacg ttagatgcgg cgatcagttc gttcacctcc tgccaagaag aacgaacaaa
    12841 tccaccgcgt ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga
    12901 agcccatgcc tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag
    12961 gcgtttgcgc atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct
    13021 ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata
    13081 gtcagtctgc tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca
    13141 ggagccggtg cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg
    13201 gcgatatcca tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa
    13261 ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct
    13321 cctgtgggag cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta
    13381 acgctctgaa acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag
    13441 taaattccta agatacccct ttaggataat taacagttac agcataacat gctgtaaatt
    13501 aagacgtttt atacctatag ttatttaaac tattaagatt tttatgcaaa tgggtattaa
    13561 ggagtattcc ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataagcca
    13621 tataacacta atatgttgta actaaatgac gcataaaaaa agcgcggcat catgccgcgc
    13681 aaaggataat caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaagta
    13741 atcactacgc aggcgatata gaaaatcaaa aatactttca ttgcacctac tggcgaaccc
    13801 gttaatgcca gcgagctacc aaacgctttc gggataaaga agccaccaat cgcgccaatc
    13861 gcagagatga aacccagcgc cgctgccgtg ccggttgccg cttcacgcat cgcacgttcg
    13921 tcagaacccc cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc
    13981 atctggaaag tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg
    14041 aagaacgcca tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg
    14101 ctgaaaatcg ccatcagaat aaagttcacc agcgtgacac gagttccgcc cagacggtca
    14161 gataatgcac cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat
    14221 tgcagaatct gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc cgcggagaag
    14281 ccgatgaagg agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt
    14341 ttgagtaccg gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca
    14401 aaccacgccg caatggtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc
    14461 agatacagct cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc
    14521 gacagtgata ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gtttcccaga
    14581 ccaccattca gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt
    14641 gccatactgg atgcgaagtt cgcgccagca aagccgcata gcagagagat gatgatgaag
    14701 acgctataag gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc
    14761 agaataccgg tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag
    14821 tatggaacac gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga
    14881 tcggtcgtaa aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc
    14941 catacgcaaa acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata
    15001 cgttgaccgc gttgttgcca gaacgcagga tcttccggtc gccaatctgt tatgacagct
    15061 ccagtagccc tttcggaggc agatgagtga ctcatagaca cctctgatac tcgtttcgat
    15121 gtctgccacc ttagtgtctg gagctaaagg caatttgatg taaatcaaac gataagataa
    15181 ctttatctat cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt
    15241 atgagatttt tcacggtgct gtagaatccc tacccttacc gatgtaaagc gactaaccac
    15301 acggcaaata aggagtaact ctttccggct atgggtatac ttcagccaat agccgagaat
    15361 actgccattc cagaatatat cgtcacattc attaaggtta ttgctcattt aaagcctgaa
    15421 ggaaggggtt tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc
    15481 gcttattgtg ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct
    15541 ggtgcaaggc gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca
    15601 aagttaccgt ctgttggcgg cagtgccatt aagcgagaaa gacaagccct taattaaaga
    15661 gatggaacaa acggcattta gcgccgagtt gactcgagca gcagaacgag acggacaact
    15721 ggcgcaatta cagggcttac aagattactg gcgtaatgaa ctgatccctg cgctgatgcg
    15781 tgcacaaaac cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca
    15841 gctggtatct ggttttgacc gcaccacgga aatgcgtatt gagacagtgg tactggtcca
    15901 tcgggtaatg gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc
    15961 gcgactgcta caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gccatcgcga
    16021 ttttacccaa cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt
    16081 gaacaatatg tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga
    16141 gaaaaccgcc gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg
    16201 ccgtttgcat tcccgcgccc cgctgtgtga acgcctgtca ccggtactca acggcttaca
    16261 gaatttaacc ctgctacgtg atatcgaact gcgggtgtat gacactgatg atgaagagaa
    16321 tcatcaggag tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg
    16381 cccgcgcggc gtattacccg ttggtgatcg cggcacgacc ctgaagtggc ggctggctga
    16441 ctctcatacg cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca
    16501 tgaccaacaa caactggtgg ataccctggt tgaacaactt accgccacgc tggcgctgga
    16561 tcgccatcag gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg
    16621 cgaactgcat gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt
    16681 acagatgcag ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa
    16741 cgaactgaat gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct
    16801 caccgagcct ggattacgtc cggcgctgga agcgagttgc gaagagtaca gcgccaaatt
    16861 tggcttcccg gtgaagctgg attatcaatt gccgcctcgt ctggtgcctt cacatcaggc
    16921 aatccacttg ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc
    16981 gagtgaggtc gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga
    17041 taacggctgt ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat
    17101 gcgcgaccgt gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg
    17161 caccgaagtg gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac
    17221 ccatgagtaa tcaggaaccg gctactatcc tgctgattga cgatcacccg atgctgcgaa
    17281 ctggcgtaaa acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta
    17341 atggcgaaca gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc
    17401 tcaatatgcc cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct
    17461 cagggcgcat tgtggtattc agcgtctcta accatgaaga agatgtggtc accgcactga
    17521 aacgcggcgc ggatggctat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat
    17581 tgcatcaggc agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg
    17641 ccagcttgcg cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg
    17701 agcgcgatat tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc
    17761 tggatatcac cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc
    17821 tcaagtctcg cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt
    17881 tcccagcgca gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc
    17941 tcattggagg agacacgctg cccctggtta tcttccacca ccacagacaa tcgccagtga
    18001 ttgcttgccc cttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg
    18061 ttggcttgtg cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc
    18121 cgaataccgt aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc aggttttaga
    18181 tcccacggcg gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc
    18241 ggtagattat ttcgctgcgg attgtcatag cgactaccac gtaacgactg actttcggca
    18301 acctcgcccg cagaaagttg ttttttgagc ggtacaccaa agcggtaatt aagattcagc
    18361 ccgaggttat tttgattttc gccactttcc ccctgtttat gctgggccgt cacagtgact
    18421 aatggcacag gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta
    18481 ccagagttaa acaaatcaac acgatcacca aaatactgtt ctaggctgac actggtattg
    18541 aggtgttgat agaacggcat gcgcatccga gctgtcaggt cgtacccgcg cgccatccgt
    18601 tgttcctgcg tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat
    18661 aatcgcaaat attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc
    18721 tgcaagttgt cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc
    18781 ccaacgccca cattgctcac caacccatta tcctgctgag taagaccaag ctggctccag
    18841 gtgagataac gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa
    18901 tgtccttcgt tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac
    18961 tctacgtgct gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct
    19021 tttgcctgct cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc
    19081 acaatttcag caaagtgttt ttccccatca tgattttcgg gagccatgcc taaatccggc
    19141 agaccatcat tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg
    19201 ttagctgtac cgcctgctac aagcaagagt aaataaaacg ggataatgcg aggaacgaaa
    19261 cggctcaaaa cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg
    19321 attactggca acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaatgtgac
    19381 cgattggtca gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata
    19441 caggagtaaa taatgcaaaa aatagtgatc gttgccaatg gcgcacctta cgggagcgaa
    19501 tccttgttta acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat
    19561 ctgcgtctgt tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg
    19621 gaaggctaca acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa
    19681 ttgtgcaaaa cctgtaccga cgggcgcggg attagcacac ttcctctgat tgatggggtg
    19741 gaaatcggta ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca
    19801 ttttaatgtt taagggggcg tctgacgccc cactatgagg gaagtttact caggcgaatc
    19861 ccggccttaa cacaccatcc ggataattct ccgccagtag tttttttacc gataccagca
    19921 aatcattcag cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca
    19981 ggtattgtgc attggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct
    20041 gagcgcgagt gtcagattca tattctggat gtcgcgggtc cataataaac acaatggcgt
    20101 ttactgtgcg tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa
    20161 tcatctcgcg cttccacaac agggttagtt cctgctccag cgtctcttct ggtagtcgat
    20221 aggcgacgcc tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg
    20281 cagtcccgcg cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca accagtgtac
    20341 cggtgcatga ttcggtaaac tccagtgccg gattccacat caacgatcca taaccgaaga
    20401 tccacaccgg tccctcatca ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt
    20461 gttctgctga ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat
    20521 tcatcaagaa atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca
    20581 actttttggt gtcttccgga catttttacc gctcattttg aaaccactaa cggaacaata
    20641 tgcaatccac ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg
    20701 ttttacgatt ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca
    20761 gcccaggcca ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcttcttta
    20821 tctttatatt gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac
    20881 ggtagaacgt gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac
    20941 tccttttaca aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc caaaacataa
    21001 gaattttcat gtcagactgt tcttaagaaa cgctaaaaca aacattgtct aaacgtaata
    21061 cttattttta tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt
    21121 accgatatcc agacactttc tgttattttt cctacctatt ttttacattt tcgataaatt
    21181 catctgcaaa gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa
    21241 ggagacttcg cttattttcc cggttctggt gctggtagtg ctgttcctgt ggggaagtag
    21301 ccagacacta ccagtggtca ttgccatcaa tcttcttgcg cttattggta ttttaagtag
    21361 cgcctttagt gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg
    21421 ttcgcttatt cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat
    21481 ggcaaccggc gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat
    21541 tgttaccggt gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca
    21601 atatatgaat ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat
    21661 cgtactggta tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact
    21721 ggtagcatta atttctgcgg caatgtatgg cgtatttttg ctgatccagg ccaaaacgca
    21781 tcaaagtttg tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcaccacgg
    21841 taaaacgtct gcccatagca gcctgtggca tgctatctgg ttgattatcc atctgattgc
    21901 cgttattgca gtcaccaaaa tgaacgccag ctcgctggag acattgctcg acagcatgaa
    21961 tgcccctgtt gcctttactg gcttcctggt ggcactgttg attctgtcgc cggaaggttt
    22021 aggtgcgtta aaagcagtgt tgaacaacca ggttcagcgc gcgatgaatc tgttctttgg
    22081 ttcagtgtta gcaacaattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac
    22141 gggtaacgaa ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt
    22201 agtgctgtgc catatctcct tctccaccgg acgtactaac gtgctcaatg gcgcagcgca
    22261 tctggcactg tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg
    22321 atgacggaaa tcgggagaga taagataaat tcctgcttat tgtatttctt catactacct
    22381 ggacagctcg ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac
    22441 agctgttgaa aagccggaat gtactcttta ttgcaaacat ttcagggatg aagtgaagat
    22501 gaattgagac gtagtatcca caccaagccg gaattatccg gtgaggcgca atgttgcggg
    22561 ggctttatcc ctggtggcat tggttgctgg aaagagaaaa cccccgcacg ttgagaggta
    22621 aaacctgaca acaaaacggg ggctaatctt gaaaccagac acttcaagaa tagccgcaaa
    22681 atgttgtcat tacaacaagg cggctatatg acgctcgcgc agtttgccat gactttctgg
    22741 cacgacctgg cagcaccgat cctggcgggg attattgccg cagcgattgt cagctggtgg
    22801 cgtaaccgga agtaaggtga aaacgggcgt caggctgccg tgatggcaat atgcgcccga
    22861 aaccggccgc aggggtaaca gcctgcggcc ttttttgtat ctggcaccca tgaaaaaagt
    22921 cttaacgcag aacgctaaga ctttattttt caagcaaaaa agattacttg ctggtatcca
    22981 gttcttcgaa acctttcacc agatcatcaa tcgctttcat ctgcttgagg aacggttcca
    23041 gtttagccag cggcagcgcg gatggaccat cacatttcgc atgttccgga tccggatgcg
    23101 cttcaataaa cagccccgcc agacctaccg ccataccggc tcgtgccagc tcagccacct
    23161 gagcacgacg accaccggaa gctgcgccaa acggatcgcg gcattgcagt gcgtgggtca
    23221 cgtcgaaaat caccggcgag ttaccagaca ctttcttcat aatgctgaag cccagcatat
    23281 cgacaaccag gttgtcatag ccgaagttag caccacgatc gcaaagaatc actttttcgt
    23341 tgccgccttc tttgaattta tcaacgatat tacccatctg tcccgggctg acaaactgtg
    23401 gtttcttgac gttaattacc gcaccggttt tcgccatggc ttcaaccagg tcagtctggc
    23461 gagcaagaaa cgccggcaac tgaatcacat ccacgacatc agcaacgggc tgtgcctgac
    23521 ttggttcgtg aacgtcggtg ataattttca cgccaaaagt ctgcttcaac tcctggaaga
    23581 ttttcatccc ttcttccagg cccggtccac gataagagtg gatggaggag cggttggctt
    23641 tgtcaaaaga ggctttgaac acgtaaggga tacccagttt ctgggtcaca gttacgtagt
    23701 gctcgcaaat gcgcatcgcc agatcgcgag attccagcac gttcataccg ccaaacagta
    23761 cgaacggcag gtcatttgct acgttgatgt cgccaatgct aaccactttt tgtttcatag
    23821 gatcgcctta atcttgagta aaatgtcgat taattaatgc agcacaatat gtttatgcgc
    23881 gatgttattt atttgcgcac ggatcatttc gctgatcggg tcttccggac accgttcaac
    23941 gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc agttgcgcat aaatcaaccc
    24001 acgatcgcga atttcatagg gatcttcagg gttgaattgt aataaagctt cgctggtgcg
    24061 taacgccagc tccatctgat tttcttccat caacgaggct ttgagtgtat ccagcaattt
    24121 gcggattacc tcaatgttat cagcttcatc aaggtcttca taaaacagtt ccgccgacgg
    24181 gctgatattt ccctttaacc atccgtccag catatgttcg cttaacgatt caccgttaaa
    24241 aggattaatc agccaaattt cgccatccgg acattcaatg cgcaatatca gctgcgtagg
    24301 gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg acccataata aaaccgcacc
    24361 taatgatacc gcactgccct gtcgattctt taacacctgg tccagccata atgcatcgga
    24421 aagacgataa acgccgcgtg aggctttaaa tccccagtcg ccgtaaaaca gcgcgatcag
    24481 tttttccagc tgctcttcta aaggcagaag ctggctgatt tcttccttcg ccagactaac
    24541 gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa tcgcggcgga ttgcttcgca
    24601 agccaggatc atgccttcgc acaatggcgc tttattaaat tcgaaatcag ctaacgatct
    24661 catgacttac cccagtaacg gtacttttgt ggtggcgagt ttaatgatga tgtacagcac
    24721 caccaacgcc agcgggaagg caataatgcg cgcctgctga ctgtgcatac gacgataatc
    24781 gagtgcaata aaacccaaaa cgatataaat gataactcca aacagctttt cagtcagcca
    24841 ctgtgcctgc ccggaaaatg gcaggatgtg cgctttagcc atcaacgcaa tgccgcttaa
    24901 caataacacc gtatcgacaa ccggcggcac aatgcgcgtc cagcgagcaa atgcctgtgg
    24961 atgcttctga taacgtagcc agaaacgtaa ggttaatagc ccaacagaaa gcgcgatact
    25021 aataagatga acactaagca gtgtagaaaa acttgtcatt gataatagcg gccgagcgtt
    25081 acgcgctcgt tatcaccata gtcacggcag gtttcgacgt catgataccc cgcgaggata
    25141 aatgcttgtc gcaccgcttc gccctgctgc cagccatgtt ccagaagcag aaagccgccg
    25201 gataccagcg cgttacgcga ctgttcgatg atatgcacga tgtctgccat tccactgtct
    25261 gccgcaacca gcgcagtgag cggctcaaag cggacatcgc cttgttgaag atgtgggtcc
    25321 tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa actgctgccc ggctagcgcg
    25381 ctaaaccagt cgctttgcag aatgtggata tttttgatcg ccagatgctg ggcattacgt
    25441 tgtgccaggg agacagcatc aggcatacga tctacagcga taatttcgca gtccgggcgc
    25501 tcgctagcca gcgcaagcgc aatcgccccg gtacccgtcc cgagatcgag aatacggcaa
    25561 ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac actccgtatc cgggcgcgga
    25621 attaaggtcg ctggcgaaac aaataacggc aacgaccaga attctcgcac cccggttaaa
    25681 tgagcaatgg gttcaccatc gcgacgacgt gtcagtagcg catcaagttg ctgacattgt
    25741 tcgtcagtca gctgcgtttc accaaaggcg agaataaaag tacgccctct gccggtaaca
    25801 tgctccagca ggatttcagc atcacgccgc gggctttcgc tcgcctgaag ttggcttatt
    25861 gcttcacgta accagtgttg atattccatt attcctgctc ggacaacgcc gccagttggt
    25921 cggcctgatg ttcctggata atcggttcaa tcagcatatc cagcttacct tccatcactt
    25981 catccaggcg gtagagcgtc aggttgatgc ggtgatcggt aacgcgcccc tgcgggaaat
    26041 tgtaagtacg gttacggtcg ctgcgatcgc cactccccag caggttacga cgggtagacg
    26101 cttcggcctg ttggcgtttt gccatttcag cagcgtggat gcgagcaccg agcactgaaa
    26161 gcgccttcgc tttgttttta tgttgtgaac gttcgtcctg acattcaaca acaatcccgg
    26221 tcggcaagtg agtaatacga attgccgaat cggtggtgtt aacgtgctga ccacccgccc
    26281 ctgacgagcg gaaagtatca atgcgtaaat ctgctgggtt gatgtccggc agttctgcgt
    26341 caggcagttc tggcattacc gcaacggtac aagcagaagt atgaatacga ccctgcgatt
    26401 ccgtagcagg aacacgttgc acgcgatgac cgccggattc aaatttcaga cgaccataca
    26461 caccatcacc gctaattttg gcgatgatct ctttataacc accatgttca ccctcgctgg
    26521 cgctcatgat ttctacccgc cagcggcggg cttcggcgta acggctgtac atacggaaca
    26581 gatcgcctgc aaacagcgct gcttcgtcgc cgccggttcc ggcacggact tcgaggaagg
    26641 cgttacgttc gtcatcagga tcttttggta gtaatagaac ctgtaattgc tgttccagtt
    26701 gctcgctttt ttctttagct tcgcgcagtt catcctgcgc catctcacgc atttcaggat
    26761 cgtcgagcat catctgtgcg gtttcgatat cttcctgaac ctgttgccag tcggtaaaac
    26821 agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa tgcgcgaaaa cgttcctggt
    26881 cggcgatagt ttgcgcgtca cccagcaacg cctgaacttc ttcatggcgt tcatgcaggg
    26941 cttccagttt ggcaacgata gaaggcttca taggcgtaaa tgcaccctgt aaaaaaagaa
    27001 aatgatgtac tgctactcca gcccgaggct gtcgcgcaga atattcaggc gttcgttatc
    27061 cccgtcacgg gcggcctgtt gaagtgattt cgttggcgca tggatcaagc ggttagtcag
    27121 tttccatgcc agatcctgca taatggcttg cgcgtcgccg ccctgctcaa gggccgctaa
    27181 cgctttggcg gttaactcat cgcgaacgtg ctctgcctgg ctgcgatact cgcgaatggt
    27241 ttcgctggcg ctttgtgctc gcagccacgc cataaattcg ctggtttcct gagcgacaat
    27301 agtttccgcc tcaaccgctg cggctttacg ctgcgccagg ttgtgcgaaa tgatgctttg
    27361 cagatcgtcc acactataaa gataagcatt cgccagtttg ccaacttccg gctcaacatc
    27421 gcgcggaacg gcaatatcca ccaacagcat tggttggtta cgacgacttt ttaatgcgcg
    27481 ctccaccatg cctttcccga taatcggtaa cgggctggcg gtggaactga tgatgatatc
    27541 ggcttcgcgc agacgttcgt cgatctcact cagggcaatc acttccgcgc caacttcatc
    27601 tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata atcatcttct gtactttatg
    27661 ttcgcgcaga tgacgcgcta ccagctcgat ggtttcgccc gcgcctacca gcaacactgt
    27721 gaccgtagag agcgattcaa agatctgccg cgccagcgta caagccgcaa aagcgacaga
    27781 cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt ttcgctacag agaaagattt
    27841 ctggaacatg cgttccagtt cgctggcctt catatgacct ttttgcgaat cgacaaacgc
    27901 ttttttaacc tgaccgagga tctgcggctc cccaagaacc agtgaatcca ggccgctggc
    27961 aacacgcatt aaatggctaa ccgcgtcgtt atcctgatgc cagtagaggc ttttacgcag
    28021 atcttcttca ttaagattgt gataatcgca aagccagcgg attaacgcct cttgcaggtt
    28081 atcctgctct tcaacgctaa gataaagttc cgtgcggttg cacgtcgaca gcaccacgcc
    28141 gccctgcacc atcggctgcg caagcaggct gtcaagcgcc tgatcgagct tatccggcga
    28201 aaacgataca cgttctcgca gcgatacagg tgccgtttta tggttgatac cgagtgctaa
    28261 aagggtcatg tctgcgggaa ataataccaa cgttgatagg gttagtctgc ttgcatcata
    28321 caggatgcgt aagatcaata aaagagagcg cccccttttg gagtaattgc cgaaagccgt
    28381 tagattctgg caattaagac aacttgaaca tagacgatag cggacggtaa cgctagcatt
    28441 aagggttata actgcaacgt atctcaagga cttgtcatca ctatgcccct gcccgatttt
    28501 cgtcttatcc gcctgctacc gctggctgct cttgtgctca ctgcctgttc cgttaccacg
    28561 cccaaaggtc ctggcaaaag cccggattcg ccacaatggc gtcagcatca gcaagacgtg
    28621 cgcaatctta atcagtatca gactcgcggc gcgttcgctt atatttctga ccaacaaaaa
    28681 gtgtacgccc gctttttctg gcagcaaacc ggccaggatc gctaccgtct gctgctcact
    28741 aacccattgg gcagcacgga actggagctg aatgctcaac cgggtaacgt gcagttagtc
    28801 gacaataaag gtcagcgtta taccgccgat gacgccgaag agatgattgg caaattgacc
    28861 ggaatgccaa ttccgctcaa cagcttgcgc cagtggattt taggtttacc gggtgatgca
    28921 accgactaca aactggacga ccagtaccgc ctgagcgaaa ttacctacag ccagaatggc
    28981 aaaaactgga aggttgttta tggtggttat gacaccaaaa cgcaacctgc gatgccagcc
    29041 aatatggaac tcaccgacgg tggtcaacgc atcaagttaa aaatggataa ctggatagtg
    29101 aaataatgcg gacacagtgg ccctctccgg caaaacttaa tctgttttta tacattaccg
    29161 gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt tcagtttctt gattacggcg
    29221 acaccatcag cattgagctt cgtgacgatg gggatattcg tctgttaacg cccgttgaag
    29281 gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg gttgttgatg aaaactgcgg
    29341 cagacagcgg gcgtcttccg acgggaagcg gtgcgaatat cagcattgac aagcgtttgc
    29401 cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc gacggtcctg gtggcattaa
    29461 atcatctctg gcaatgcggg ctaagcatgg atgagctggc ggaaatgggg ctgacgctgg
    29521 gcgcagatgt tcctgtcttt gttcgggggc atgccgcgtt tgccgaaggc gttggtgaaa
    29581 tactaacgcc ggtggatccg ccagagaagt ggtatctggt ggcgcaccct ggtgtaagta
    29641 ttccgactcc ggtgattttt aaagatcctg aactcccgcg caatacgcca aaaaggtcaa
    29701 tagaaacgtt gctaaaatgt gaattcagca atgattgcga ggttatcgca agaaaacgtt
    29761 ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata cgccccgtca cgcctgactg
    29821 ggacaggggc ctgtgtcttt gctgaatttg atacagagtc tgaagcccgc caggtgctag
    29881 agcaagcccc ggaatggctc aatggctttg tggcgaaagg cgttaatctt tccccattgc
    29941 acagagccat gctttaagcc gggcaagctg agtttcggtg acaacgtcac cttgttccag
    30001 acgttgcatc gcgctcttta atacaccgcc tggaaaggat catgcctggc ccgcacagtt
    30061 ttcggcagat tctttccacc aatggacgca tgcctgaggt tcttctcgtg cctgatatga
    30121 agctttttgc tggtaacgcc accccggtac tagcacaacg tattgccaac cgcctgtaca
    30181 cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg cgaagtcagc gtacaaatta
    30241 acgaaaatgt acgcggtggt gatattttca tcatccagtc cacttgtgcc cctactaacg
    30301 acaacctgat ggaattagtc gttatggttg atgccctgcg tcgtgcttcc gcaggtcgta
    30361 tcaccgctgt tatcccctac tttggctatg cgcgccagga ccgtcgcgtc cgttccgctc
    30421 gtgtaccaat cactgcgaaa gtggttgcag acttcctctc cagcgtcggt gttgaccgtg
    30481 tgctgacagt ggatctgcac gctgaacaga ttcagggttt cttcgacgtt ccggttgata
    30541 acgtatttgg tagcccgatc ctgctggaag acatgctgca gctgaatctg gataacccaa
    30601 ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg cgctatcgct aagctgctga
    30661 acgataccga tatggcaatc atcgacaaac gtcgtccgcg tgcgaacgtt tcccaggtga
    30721 tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact ggtcgatgat atgatcgaca
    30781 ctggcggtac gctgtgtaaa gctgctgaag cgctgaaaga acgtggtgct aaacgtgtat
    30841 ttgcgtacgc gactcacccg atcttctctg gcaacgcggc gaacaacctg cgtaactctg
    30901 taattgatga agtcgttgtc tgcgatacca ttccgctgag cgatgaaatc aaatcactgc
    30961 cgaacgtgcg tactctgacc ctgtcaggta tgctggccga agcgattcgt cgtatcagca
    31021 acgaagaatc gatctctgcc atgttcgaac actaatcgaa cccggctcaa agacccgctg
    31081 cggcgggttt ttttgtctgt aatatccatt tgtatgacct atgcctcctt cacctgccat
    31141 ttagttgaca gatgatgcgc tcacggatga aacattattg tgaacaaaat attttcctca
    31201 catgtgatgc ctttccgcgc tctgatcgac gtttgctgga aagaaaaata tactgccgca
    31261 cggtttaccc gtgacctgat tgccgggata accgtcggga ttattgctat cccgctggcg
    31321 atggcgttgg ctattggtag tggtgtggcc ccccagtacg gtttatatac cgcagctgtt
    31381 gcggggattg tcattgctct aacgggtggg tcacgcttta gcgtttccgg tccgactgcg
    31441 gcatttgtgg taattctcta tcccgtttcg caacagtttg gactggcagg actgctggtt
    31501 gcgaccttgt tgtcgggaat ctttttgatt ctgatggggc tggcacgctt tggtcgtctg
    31561 attgagtata ttccggtttc cgtcacctta ggtttcacct cgggtatcgg gatcaccatc
    31621 ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg cccatgtccc ggaacattat
    31681 ctacaaaaag tcggcgcatt atttatggcg ctgccgacca ttaatgtggg tgatgctgcc
    31741 attggcattg tgacgcttgg cattctggtt ttctggccgc gtctgggcat tcgtttaccc
    31801 ggtcaccttc cggccttgct ggctggttgc gcggtgatgg ggattgttaa cctgctcggc
    31861 ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc tggccgatgg ttctcagggt
    31921 aacggtattc cgcaactgct gccgcaactg gtgctgccgt gggatctgcc taattcagaa
    31981 ttcacgctaa cctgggattc tattcgcacc ctgctgcctg cggcattctc aatggcaatg
    32041 ctcggggcga tcgaatctct gctctgcgct gtggtactgg atggtatgac cgggactaag
    32101 cacaaggcga atagtgaact ggtcggtcag gggctgggga atatcatcgc tccattcttt
    32161 ggtggtatta ccgctactgc tgccatcgcg cgttctgccg ctaacgtccg tgccggggca
    32221 acttccccta tctcggcggt gatccactct attctggtta ttctcgccct gctggtactg
    32281 gcaccactgc tctcctggct gccgctttcc gccatggcag ccctgctgtt gatggtggcg
    32341 tggaacatga gtgaagcgca caaagtggtc gacttgctgc gtcatgcgcc gaaagatgac
    32401 atcatcgtca tgctgctgtg tatgtcactg accgtgctgt ttgatatggt tattgccatc
    32461 agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc gtatcgcacg tatgactcgc
    32521 ctggcaccgg tagtcgtaga tgttccagac gatgtcctgg ttctgcgcgt tattggcccg
    32581 ctgttttttg ctgctgctga aggcttattc acggacctgg agtcacgtct tgaaggcaaa
    32641 cggattgtga ttctgaagtg ggatgccgtt ccggtacttg atgctggtgg tcttgatgcg
    32701 ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac tgcgcgtgtg caacgtggaa
    32761 ttccagccac tgcgaactat ggctcgcgca ggcattcaac cgatcccggg acgcctcgcg
    32821 ttcttcccga atcgtcgcgc ggcgatggcg gatttataag tacgagattg accagtcagc
    32881 gcaaactgac tggtcagcaa actgcattat ttgttagcta tgacggcgat tatcgctacg
    32941 gcggcaacgt ttgtcatagt gacgaaccca ccagtaacgg tcgcacacct gttcatgccc
    33001 gccaacacgg gcacccgcca gccagagaat ggcaccgacg aaaatactta gcactgcacc
    33061 atgtgcgaaa tactggggca tattaaactg tggtaactgg ttgaggattg aataccccac
    33121 gccgaccacc attaccacca gacccaaccc catgagcacg ttaccgagta tcgaagcgtt
    33181 tttgcgtttc atatgtcacc tccggaactt tctgggttgt aacagggaat acccctcttc
    33241 cttatgtgta aagtatagac aacaccctgt gggttatgtg cgggcgtaat cacaattaca
    33301 acccttattt caacaaaact ttacaaataa acgcctgaac accttgactt tctaatagtc
    33361 cactcaggca attattacgt tatctgcatt tgatacgcag ttttttgtca agcggggccg
    33421 caaccagtaa actacgcgcc agttatgtac acactcagga caaaaaaacg tgacgattaa
    33481 attgattgtc ggcctggcga accccggtgc tgaatacgcc gcaacgcgac ataatgctgg
    33541 tgcctggttc gttgacttac tggcagagcg tttgcgcgct ccgctgcgcg aagaggctaa
    33601 attctttggt tatacttcgc gagtcaccct tggaagcgaa gatgtccgcc tgttagtccc
    33661 gactacattt atgaatctca gcggcaaagc cgttgcggcg atggccagtt ttttccgcat
    33721 taatccggac gaaattctgg tggcccacga cgaactggat ctgcctcctg gcgtcgccaa
    33781 atttaagttg ggcggtggcc atggtggtca caatggactg aaagacatca tcagtaaatt
    33841 gggtaataac cctaactttc accgtttacg catcggaatc ggtcatccgg gcgataaaaa
    33901 taaagttgtc ggttttgtgt taggcaaacc gcctgttagt gaacagaagt taattgatga
    33961 agccatcgac gaagcggcgc gttgtactga aatgtggttt acagatggct tgaccaaagc
    34021 aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt gtctgcggca tttttgccga
    34081 gtgccgtgta taataggcaa agttatttcc atttctgcaa tctgttagca ataacaggtt
    34141 gattattaag atattaaggt gatttaaatc atgggattca aatgcggtat cgtcggtttg
    34201 cccaacgtcg ggaaatctac cctgttcaac gcgctgacca aagccggtat tgaagcggcc
    34261 aactttccat tctgcaccat tgagccgaac acaggcgtcg taccaatgcc tgaccctcgc
    34321 ctggatcaac tggctgaaat cgtaaaaccg caacgtacgc ttcccacgac catggaattt
    34381 gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg aaggtctggg taaccagttc
    34441 ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg ttcgctgctt tgaaaatgac
    34501 aacatcattc acgtttccgg caaagttaac ccggctgacg atattgaagt tatcaacacc
    34561 gaactggcgc tggcagacct cgacacctgc gaacgtgcga ttcatcgcgt acagaagaaa
    34621 gccaaaggtg gcgataaaga cgcgaaagct gagctggcgg tcctggaaaa atgcctgccc
    34681 cagctggaaa acgcaggtat gctgcgcgcg ctggatttaa gcgctgaaga gaaagcggct
    34741 attcgttacc tgagcttcct gacgctgaaa ccaacaatgt acatagccaa cgtcaacgaa
    34801 gatggttttg aaaacaaccc atatcttgac caggtgcgtg aaatcgcggc gaaagaaggt
    34861 tctgttgtgg ttccggtttg tgctgctgtt gaagcagaca ttgccgaact ggacgacgaa
    34921 gaacgtgacg agtttatgca ggagcttggg ctggaagaac cgggcctgaa ccgtgtgatc
    34981 cgtgccggtt ataagctgct gaacctgcaa acttacttca ccgctggggt gaaagaagtg
    35041 cgtgcatgga ccattccggt tggagcaacc gcgccgcagg cagcgggcaa aatccatact
    35101 gattttgaaa aaggctttat ccgtgcacaa accatctcgt ttgaagattt catcacttac
    35161 aaaggtgaac aaggcgcgaa agaagcaggc aaaatgcgtg cagaaggtaa agattacatc
    35221 gtgaaagatg gcgatgtgat gaacttcctt ttcaacgtct aattaattta ttgtctcatg
    35281 aggtttcgct aaatctcatg aagagcgcaa aagtatataa aatccacgca attgcgtgga
    35341 tttttcattt caccgagtcc taatcattct tgtaacatcc tacacttttg tgcttattgc
    35401 ctacagtgaa ccacgagaat aattaaagac ataagtgttc agatgactga tatctcatta
    35461 gcagtgatat aacgtcagtt tttgttccac tacacatcag ccctcttcac agctgatcaa
    35521 tattgatctc gctccaatat gctggcgaga aaaggactta cctgaagggt aatatcccgc
    35581 agataaagtc cccgaataac gtgattacgt gcgatcctgg caagatgaaa atgataacca
    35641 gtagcacaat aataacgata ggccagaaaa tccattgaac aaaaatgaat atcgatgttt
    35701 tttgcttttt cttattagta atgcaaaaat ggcgcatatt gtttttgtta tgcagataca
    35761 tcaagcgaaa atcccataac taattaggat tattacaaaa aatcttcacc aaatttttta
    35821 accgctgttt gttgtaatat ttttgccata ttcatagtaa cgaattggaa taagtaacga
    35881 caacttaggg aataactcga tataaactca cctttgtaag gggggggtta aatatatatt
    35941 aattcctgtg ttcctgattt tgcccggtaa tagagaatta ttctcagaga agctcatcat
    36001 gattgcacca tacagatatc atgatgataa gaggatatta ttcattgact taaacaaaat
    36061 gacaaaatcc atagacccat agcctgggta ctatatagga gtggtatggg catcaaacaa
    36121 cacaatggga ataccaaagc cgatcgtctc gctgaattaa aaatccgttc gccctcaatt
    36181 caactgataa aatttggcgc tattggtttg agtgcaatta tcttttcccc cctgctgata
    36241 gctgctgata caggaagtca atatggcacc aatattacta ttaatgatgg tgacagaatt
    36301 acaggagata ccgccgatcc atcaggaaac ctctatggtg taatgacccc agcaggaaac
    36361 acgcctggca atatcaacct gggtaatgat gtcaccgtca atgtcaacga cgcctctgga
    36421 tatgcaaaag gaatcattat tcagggcaaa aacagctccc tgacagctaa ccgactcaca
    36481 gtagatgttg ttggtcaaac ctctgccatc ggcattaact taattggtga ctatacccat
    36541 gctgacttag gcacaggcag caccattaag agtaacgatg acggcatcat tattgggcat
    36601 agctcaacac taacagccac tcaattcacc attgaaaact cgaacggtat aggcctaacc
    36661 atcaatgact atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa gaccgatgga
    36721 agtacaggtg tttatatcgg tggtctcaac ggcaataacg ccaatggtgc tgcgcgtttt
    36781 acggcgacag acctgacaat cgatgttcag ggctacagcg ccatggggat aaacgtacag
    36841 aaaaactctg ttgtcgatct cggaacaaac agtaccatta aaaccaatgg cgataatgct
    36901 cacggcctct ggagctttgg ccaggttagc gcgaatgcac tcactgttga tgtaactgga
    36961 gccgcggcca atggcgtcga agttcgtggt ggtacaacca ctatcggtgc agatagccat
    37021 atttcttccg cgcagggcgg tggcctcgtc accagtagtt cagacgcgac aatcaatttt
    37081 tctggcacgg cagcgcaacg aaacagcatc ttttccggcg gttcttatgg tgcctcggcc
    37141 cagacggcaa cggctgttat caacatgcaa aataccgata ttacggttga tcgtaatggc
    37201 agtctggcgc tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga cagtttggct
    37261 atcaccggcg cggcaggagc cagagggatt tatgccatga ccaacagcca gatcgacctc
    37321 acgagcgatc tggtcattga tatgagtaca cccgaccaga tggccatcgc aacgcaacat
    37381 gacgatggtt atgccgccag ccgcatcaac gcctcgggtc gtatgcttat caacggtagc
    37441 gttctttcca aaggtgggct aatcaatctg gatatgcacc ctgggtcggt ttggacaggt
    37501 tcctccctca gcgataatgt caatggcggg aaactggacg ttgcaatgaa taacagcgtc
    37561 tggaacgtaa caagtaattc taatctcgac acgctggcgc tgagccattc aactgtcgat
    37621 tttgccagcc acgggtcaac tgccggcaca tttaccacat taaacgtaga gaacctgagc
    37681 ggtaacagta cctttattat gcgtgctgat gttgttggcg agggtaatgg cgttaataat
    37741 agaggggatt tattgaatat cagcgggagt agtgctggta atcacgtatt ggctatccgc
    37801 aaccagggca gcgaggccac aacgggaaat gaagttctga cagtggtaaa aaccactgac
    37861 ggcgcggcct cgttcagcgc gtcttctcag gttgagttgg ggggatatct gtacgatgtg
    37921 cgtaaaaatg gcactaactg ggagctttac gcttccggga cagttccgga accgactcct
    37981 aatcctgaac ccacaccagc tcccgctcag cctcccatag tcaaccccga tcctacgcct
    38041 gaacccgctc ccacgcctaa acccaccacg accgcagatg ctggcggcaa ttatctcaat
    38101 gtcggttact tattgaacta tgttgaaaac cgtacgctga tgcaacggat gggtgacttg
    38161 cgaaatcaga gtaaagacgg taatatctgg ttgcgcagtt atgggggaag cctggactcc
    38221 tttgccagtg gcaaactgag cggctttgac atgggttaca gcggtatcca gtttggtggg
    38281 gataaacgtc tctctgatgt aatgccgttg tatgtcggtc tgtatattga ctcaacacat
    38341 gcatcgccgg actatagcgg aggcgacggt accgcacgtt cagactacat gggaatgtac
    38401 gccagttaca tggcacaaaa cggtttttac agcgatctcg ttataaaagc atcgcgccag
    38461 aaaaatagtt tccacgtact ggacagtcag aacaacggcg ttaacgccaa cggcactgcg
    38521 aatggaatga gcatctccct ggaagccggg cagaggttca acctgtcccc tactggttat
    38581 gggttctata tagagccgca aacccagctt acatacagcc accagaatga gatggctatg
    38641 aaggcgagta atggcctcaa tatacatctg aatcactacg aatcgctgct ggggcgtgcc
    38701 agcatgatac tggggtatga catcaccgca ggcaacagcc agctgaatgt ctatgtgaag
    38761 actggcgcta tccgcgagtt ttcaggggat accgaatatc tgttgaacga ctcccgggag
    38821 aagtacagtt tcaaaggtaa tggctggaat aacggcgtgg gagtcagtgc acagtataac
    38881 aaacagcaca cattctatct cgaagcggat tacacgcagg gtaacctctt tgatcagaag
    38941 caagtcaacg gaggatatcg cttcagcttt taagtgcggg atagacaagc ccttaaacga
    39001 tgctgaaaat tgttagcgtg cgccggaatg aaatacagat cactctgatg tatcagaaat
    39061 cgtttctgtc tttcataccc ggcgcttaaa ctgccctgca tcaatgccat gttgcttcat
    39121 tttccgccac agcgtagtgc ggccgatccc aagtaaagcc gacatttcct gaatgcgacc
    39181 gcctgtgacc tgggctgcgt taataattgc ctctttttca acttccgcaa atgacagact
    39241 ggtggaaagg cgggtggcgc tgacatcatc tgtcgcctgc tcggtaaaca gatgttcagg
    39301 taaatcactg acgcgaatgc gcccgttatc actactcaga gcaagattct cgatgacgct
    39361 gtaaagttca aaatcgttgc ctggccatgc acaagaaact aggcgagcga gggcatcgtc
    39421 atcaattttc agccgcgtag agaagcgttt ttcaagactg cgtaatttgt tattcaccag
    39481 cgccggaatg ctgccacgcc gcatacgcag aggcgggatg gtaatttcaa atgcatgcag
    39541 cgcgtaatac agctggcgac taaaacgatt ttgttccacc agcattgcga ggtccgcggt
    39601 cgttgttgca atcactttga catcaattgg tattaaacgc cgcgcatcca gtcgcgtgat
    39661 aaccccctgc ttgataacct gcagcaaagc agactgtaac tccaccgcca gatattcaat
    39721 cttttcaaga aacagcgtgc cgccgtgtgc cagttccagc cgactcagac ggccattttc
    39781 attgtccgtg cgatcgccac caataaattc ttccgccagc gcagcatcac catataactc
    39841 acaattgacg gcgatataag gacctgcagc acgctcgctt tcattatgaa ttgcctgact
    39901 tagcagtgcc ttgcccacgc cctcttctcc acaaagcagg acaggaaagc tactgcgcgc
    39961 cgcctggcga ccaaaatgaa tcaagcggcg ggtttgcgga tcgtcctgtg gcatatgagc
    40021 gaaggtatgg ctgacttttc ctaattgact ggtcattaac tgccgcatct gttccacagg
    40081 atggagcaac aaaataaagc tggttccctg cgtttctatt atcggtttaa gggttatcac
    40141 cgcatcaata aactggtgct ggctttcaaa ggttgcttct acgtgtttga gcggatgtgc
    40201 cagttttatt gcttgttgca atacggcggg taacgttaag agttcagtga ttgcccgtcc
    40261 ctgacttgcc gtcgcgtcaa ggcgcaagac ccgcgccgcc tgggcattaa taaattgcaa
    40321 attaccctgc tcgtcccagc taatcacgcc atcatccata ctttctaaca gggcattaag
    40381 ttgatttaaa tgacggttag tttcagcgag caaactgtcc gtcagcagta aattaccgac
    40441 ctcgcgggcg attgccaacg tcaacggcaa atcagctgcg gtagtttgct caaccggaca
    40501 cgccagcgct attgttcccg tcaatcggcc cttgctgtca aacaacggcg ttgcacaaaa
    40561 ggcccagttc cagagtgcct gtttgaaatg ttgatcggcc atcgttttca cggcctgacc
    40621 agagatagcc gctaacgata gcgcacaagt accaataatt ccctcggcgc aatacgtgcc
    40681 gtcattgaat cccagtgcac ttagctgctg caaggtttgc ggatcgccat tacggctgag
    40741 aatgcaggcg gtttcatcga ggataaacag cgcacactct cgcggtgcca tatattccca
    40801 ggcatcttcc agcgcagcct gcccgagcgt cagcatcgct ttcttacgcc gataaataga
    40861 agcaaatgtc acgccctggg cctgatgtgg tacgttccat gtctcccgtt tcatcagctt
    40921 attgcatcgc tcccaggagg ttgcaattaa gggagatatg ccccgaccat cgttgttaaa
    40981 agcgccactc atatcccgca tcccttatga caaaaggtgt tccggctcgc aattttaagt
    41041 attaatacac gtaaaagttt caatatggaa cattcattaa agcatatgtt tcactatgaa
    41101 acataatttg ggctaatttt cttttccctt gcctgatgca caatggattc atcggcagtc
    41161 agtggtcgcc gtgtcgttga acatcatcca tgccctaccg taattgctgg agcaaaataa
    41221 tgaaaaaatt gatcaatgat gtgcaagacg tactggacga acaactggca ggactggcga
    41281 aagcgcatcc atcgctgaca ctgcatcagg atccggtgta tgtcacccga gctgatgccc
    41341 ctgttgcagg aaaagtcgcc ctgctgtcgg gtggcggcag cggacacgag ccgatgcact
    41401 gtggttatat cggtcagggg atgctttcgg gggcctgtcc gggcgaaatt ttcacctcac
    41461 cgacgcccga taaaatcttt gaatgctcca tgcaaattga tggcggcgaa ggtgtactgt
    41521 tgattatcaa aaattacacc ggcgatattc ttaactttga aacagcgacc gagttactgc
    41581 acgatagcgg cgtaaaagtg accactgtgg tcattgatga cgacgttgcg gtaaaagaca
    41641 gtctttatac cgccgggcga cgcggcgttg ccaacaccgt attaattgaa aaactcgtag
    41701 gcgcagcggc ggagcgtggc gactcactgg acgcctgtgc ggaactgggg cgtaagttga
    41761 ataatcaagg ccactcaata ggtatcgctc tcggtgcctg taccgttcct gccgcgggca
    41821 aaccttcttt tacccttgcg gataatgaga tggagtttgg cgtaggcatt catggtgagc
    41881 caggtattga ccgccgccct ttctcttccc ttgatcaaac cgtcgatgca atgttcgaca
    41941 ccctgctgga acatggctca taccatcgca ctttgcgttt ctgggattat caacaaggca
    42001 gctggcagga agaaccacaa accaaacaac cgctccagtc tggcgatcgg gtgattgcac
    42061 tggttaacaa tcttggcgca actccgcttt ctgagctgta cggcgtctat aaccgcctga
    42121 ccacacgttg ccagcaagcg ggattgacta tcgaacgtaa tttaattggc gcgtactgca
    42181 cctcactgga tatgaccggt ttctcaatca ccttactgaa agttgatgac gaaacgctgg
    42241 ctctctggga cgccccggtc cacaccccgg cccttaactg gggtaaataa ggagaaagca
    42301 atgtcactga gcagaactca aattgttaac tggctcactc gttgtggcga tattttcaac
    42361 accgagagcg agtatcttac cgggctggat cgcgagattg gcgacgctga ccacgggcta
    42421 aatatgaacc gaggctttag caaagtggtg gaaaaactcc ctgctatcgc agataaagat
    42481 atcggtttca ttctcaaaaa taccggtatg acgctgcttt ccagcgtcgg tggtgccagt
    42541 ggtccgctgt tcggtacctt ctttatccgc gccgcacagg cgacccaggc acggcaaagc
    42601 ctgacactgg aagagcttta tcagatgttc cgcgatggcg cggacggcgt aatcagtcgc
    42661 gggaaagccg aacctggcga taaaaccatg tgtgatgtgt gggtgccggt ggtggaatcg
    42721 ttacgtcagt ccagcgagca aaatctctct gttccggtgg cgctcgaagc tgccagtagc
    42781 atcgccgaat ccgctgcaca aagtacgatt acgatgcaag cccgcaaagg ccgcgccagt
    42841 tatctcggtg aacgcagtat tggtcaccag gatcccggcg cgacctcggt gatgtttatg
    42901 atgcaaatgt tggcgttagc cgcaaaagag taaggaattg gtgatggtaa acctggtcat
    42961 agtttcacat agcagccgac tgggagaagg tgtcggtgaa ttagcccgtc agatgttaat
    43021 gagtgatagt tgtaaaatcg ccattgccgc gggaattgac gatccacaaa atcccattgg
    43081 taccgatgcc gtcaaagtga tggaggccat cgaatctgtt gctgatgccg accatgtgct
    43141 ggtcatgatg gatatgggta gcgcattatt gagtgctgaa actgcgctgg aattgctggc
    43201 tcccgagatc gccgcaaaag tacgtttgtg tgctgcgccg ttggtcgaag gtacactggc
    43261 agcaacggtc agcgcggcct cgggggcgga tatcgacaaa gttatctttg acgctatgca
    43321 tgcgctggaa gccaaacgtg aacaactggg tttaccgtcc tccgacactg aaatctctga
    43381 cacatgtcct ccgtacgatg aagaagcccg ttctctggcg gtggtcataa aaaaccgtaa
    43441 cggcctgcat gtacgtccgg cctcccggct ggtttatacc ttatcgacat ttaatgccga
    43501 tatgttgctg gaaaaaaacg gcaaatgcgt cacaccagag agtattaacc agattgcgtt
    43561 actacaagtt cgctataacg atacgctgcg cctgattgcg aaagggccag aagctgaaga
    43621 ggcactgatc gctttccgtc acctggctga agataacttt ggtgaaacgg aggaagtcgc
    43681 tccacctact ctgcgtcccg ttctgcctgt ttcgggtaaa gccttttatt atcaaccagt
    43741 tttatgtacg gtacaggcaa aatcaaccct gaccgcggaa gaagaacaag atcgattacg
    43801 ccaggctatt gacttcacgt tattagatct gatgacgtta acagcgaaag cagaagccag
    43861 cgggcttgac gatattgccg caatcttttc tggtcaccat acactgttag gtgatccgga
    43921 actgctggcg gcggcaagcg aactccttca gcatgaacat tgcacggcag aatatgcctg
    43981 gcagcaagtt cttaaagaac ttagccagca ataccagcaa ctggatgatg aatatctaca
    44041 agctcgctat attgatgtgg acgatcttct gcatcgcacc ctggtccacc tgacccaaac
    44101 gaaagaagaa ctcccgcagt ttaactcgcc aactattcta ctggcggaga acatttatcc
    44161 ttccacagta ctgcaactgg atccggcggt tgtaaaaggt atctgcctta gcgccggaag
    44221 tccggtatcc cacagcgccc taatcgcccg tgaactgggg attggctgga tttgccagca
    44281 gggtgagaaa ctgtatgcga tacaaccaga agaaacgcta acgctggacg ttaaaacgca
    44341 acgtttcaac cgtcagggtt aagccaccgt tcggatggca tcgttctgat gtcatccggc
    44401 aatttaccca gcactgatgt tttttaacta cctgttatat tttttaccaa tttatgatga
    44461 tttttcttcg ctaatcgcga accaaatgtc gctggctggc taataaacag acgttatttg
    44521 atcactctat ttcgccgtta tttatccctt taattccctt ttctaaaatg cctgaccgtt
    44581 cgcagaatga gatttcgatc atgcagctag tgcgatcctg aactaaggtt ttctgatact
    44641 tgaataccgt tttattcggt ttcgccaaag gagaatgatt gatgaaatcc cccgcacctt
    44701 ctcgcccgca aaaaatggcg ttaattccag cctgtatctt tttgtgtttc gctgcgctat
    44761 cggtgcaggc agaagaaaca ccggtaacac cacagccgcc tgatatttta ttagggccgc
    44821 tgtttaatga tgtgcaaaac gtcaaacttt ttccggacca aaaaaccttt gccgatgccg
    44881 tgcctaacag cgatccgctg atgatccttg ctgattatcg gatgcagcaa aaccagagcg
    44941 gatttgatct gcgccatttc gttaacgtca atttcaccct gccgaaagaa ggcgagaaat
    45001 atgttccgcc agaggggcag tcactgcgcg aacatattga cggactttgg ccggtattaa
    45061 cgcgttctac cgaaaacacc gaaaaatggg attctctgtt accgctgccg gaaccttatg
    45121 tcgtgccggg cggacgtttt cgcgaggtat attactggga caattacttc accatgttag
    45181 gacttgccga aagtggtcac tgggataaaa tcgcggatat ggtggccaat tttgctcatg
    45241 aaataaacac ttacggtcat attcccaacg gcaaccgcag ttactattta agccgctcgc
    45301 aaccgccctt ctttgccctg atggtagagt tactggcgca gcatgaaggc gatgccgcgt
    45361 tgaagcaata cctgccgcaa atgcaaaaag aatatgctta ctggatggac ggtgttgaaa
    45421 acctgcaagc cggacaacag gaaaaacgcg ttgtaaaact tcaggatagt acccttctca
    45481 accgctactg ggacgatcgc gatacgccac gaccagagtc atgggtggaa gatattgcca
    45541 ccgccaaaag caatccgaat cgacctgcca ctgaaattta ccgcgacctg cgctctgccg
    45601 ctgcgtctgg ctgggatttc agctcgcgct ggatggacaa cccgcagcag ttaaatacct
    45661 tacgcaccac cagcatcgta ccggtcgatc tgaacagcct gatgtttaaa atggaaaaaa
    45721 tcctcgcccg cgccagcaaa gctgcaggag ataacgcgat ggcaaaccag tacgaaacgc
    45781 tggcgaatgc ccgtcaaaaa gggatcgaaa aatacctgtg gaacgatcaa caaggctggt
    45841 atgccgatta cgacctgaaa agtcataaag tgcgcaatca gttaaccgcg gccgccctgt
    45901 tcccgctgta cgtcaatgcg gcagcgaaag atcgcgccaa caaaatggcg acggcgacga
    45961 aaacacatct gctgcaaccc ggcggcctga acaccacgtc ggtgaaaagt gggcaacaat
    46021 gggatgcgcc aaatggctgg gcaccgttac agtgggtcgc gacagaagga ttacaaaact
    46081 acgggcaaaa agaggtggcg atggacatta gctggcactt cctgaccaat gttcagcaca
    46141 cctatgaccg ggagaaaaag ctggtggaaa aatatgatgt cagcaccacc ggaacggggg
    46201 gcggcggtgg cgaatatcca ttacaggatg gctttggctg gaccaatggc gtgacgctga
    46261 aaatgctgga tttgatctgc ccgaaagagc aaccgtgtga caatgttccg gcgacgcatc
    46321 cgaccgttaa gtcagcaacg acgcaaccct caaccaaaga ggcacaaccc acaccttaac
    46381 cagcgcttac tccgacaacg tcattcggct tctccggatg gcgttgtcgg cagataacac
    46441 catatctatg gttttttcta cacagacggt ttttcaagtc ctggcgggaa gtattgcgca
    46501 aatgtttgcc atacgtggtt gccgaagata gcgtcatttc tacgggtaag tgtttcactt
    46561 tcatcacccg gcgatttacg ccgacgttgg ctatataaag catcaattgc taccgaatat
    46621 tgttacggtg ttcactcatc aacgtttttt tgctttctgg agttaaatta ataatcacat
    46681 aatccagaca ctccagagaa tttagcaacc aaaaaaagaa ttcagaccat tcctcttcgc
    46741 gtaactgaag cattgccgaa cgggt
//
LOCUS       DAJTYL010000017        45970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYL010000017 DAJTYL010000000
VERSION     DAJTYL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.017"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="OO572_000952"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="OO572_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6660041.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="OO572_000953"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="OO572_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6660042.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="OO572_000954"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="OO572_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6660043.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="OO572_000955"
     CDS             complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="OO572_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6660044.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="OO572_000956"
     CDS             complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="OO572_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6660045.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="OO572_000957"
     CDS             complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="OO572_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6660046.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="OO572_000958"
     CDS             complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="OO572_000958"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6660047.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="OO572_000959"
     CDS             complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="OO572_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6660048.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="OO572_000960"
     CDS             complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="OO572_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6660049.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="OO572_000961"
     CDS             complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="OO572_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6660050.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="OO572_000962"
     CDS             complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="OO572_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6660051.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="OO572_000963"
     CDS             complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="OO572_000963"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6660052.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12571..14487)
                     /gene="mngA"
                     /locus_tag="OO572_000964"
     CDS             complement(12571..14487)
                     /gene="mngA"
                     /locus_tag="OO572_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6660053.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="OO572_000965"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="OO572_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6660054.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="OO572_000966"
     CDS             complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="OO572_000966"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6660055.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="OO572_000967"
     CDS             complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="OO572_000967"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6660056.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="OO572_000968"
     CDS             complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="OO572_000968"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6660057.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="OO572_000969"
     CDS             complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="OO572_000969"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6660058.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21945..22661)
                     /gene="sdhB"
                     /locus_tag="OO572_000970"
     CDS             complement(21945..22661)
                     /gene="sdhB"
                     /locus_tag="OO572_000970"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6660059.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="OO572_000971"
     CDS             complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="OO572_000971"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6660060.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="OO572_000972"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="OO572_000972"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6660061.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="OO572_000973"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="OO572_000973"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6660062.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25569..25676
                     /locus_tag="OO572_000974"
     CDS             25569..25676
                     /locus_tag="OO572_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660063.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25882..27165
                     /gene="gltA"
                     /locus_tag="OO572_000975"
     CDS             25882..27165
                     /gene="gltA"
                     /locus_tag="OO572_000975"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6660064.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27555..28121
                     /locus_tag="OO572_000976"
     CDS             27555..28121
                     /locus_tag="OO572_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6660065.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28172..30631
                     /locus_tag="OO572_000977"
     CDS             28172..30631
                     /locus_tag="OO572_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6660066.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30646..31377
                     /locus_tag="OO572_000978"
     CDS             30646..31377
                     /locus_tag="OO572_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6660067.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31374..32450
                     /locus_tag="OO572_000979"
                     /pseudo
     CDS             31374..32450
                     /locus_tag="OO572_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32602..33648
                     /locus_tag="OO572_000980"
     CDS             32602..33648
                     /locus_tag="OO572_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6660068.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33645..34436)
                     /gene="nei"
                     /locus_tag="OO572_000981"
     CDS             complement(33645..34436)
                     /gene="nei"
                     /locus_tag="OO572_000981"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6660069.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34472..35206)
                     /gene="pxpA"
                     /locus_tag="OO572_000982"
     CDS             complement(34472..35206)
                     /gene="pxpA"
                     /locus_tag="OO572_000982"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6660070.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35196..36128)
                     /gene="pxpC"
                     /locus_tag="OO572_000983"
                     /pseudo
     CDS             complement(35196..36128)
                     /gene="pxpC"
                     /locus_tag="OO572_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36122..36778)
                     /gene="pxpB"
                     /locus_tag="OO572_000984"
     CDS             complement(36122..36778)
                     /gene="pxpB"
                     /locus_tag="OO572_000984"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6660071.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36801..37544)
                     /gene="ybgI"
                     /locus_tag="OO572_000985"
     CDS             complement(36801..37544)
                     /gene="ybgI"
                     /locus_tag="OO572_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6660072.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37815..39296
                     /gene="dtpD"
                     /locus_tag="OO572_000986"
     CDS             37815..39296
                     /gene="dtpD"
                     /locus_tag="OO572_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6660073.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39446..40864)
                     /gene="phrB"
                     /locus_tag="OO572_000987"
     CDS             complement(39446..40864)
                     /gene="phrB"
                     /locus_tag="OO572_000987"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6660074.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(40861..41370)
                     /locus_tag="OO572_000988"
     CDS             complement(40861..41370)
                     /locus_tag="OO572_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6660075.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41499..41786
                     /locus_tag="OO572_000989"
     CDS             41499..41786
                     /locus_tag="OO572_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6660076.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(41841..42221)
                     /locus_tag="OO572_000990"
     CDS             complement(41841..42221)
                     /locus_tag="OO572_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660077.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42224..45610)
                     /locus_tag="OO572_000991"
     CDS             complement(42224..45610)
                     /locus_tag="OO572_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6660078.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45629..45832)
                     /locus_tag="OO572_000992"
                     /pseudo
     CDS             complement(45629..45832)
                     /locus_tag="OO572_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11019 a        12526 c        11127 g        11298 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 tcgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagctgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacttgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttatcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctac ctcggctttt
     3661 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3721 gccgctttct tcgctgcttc ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc
     3781 acttcagctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
     3841 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
     3901 tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa
     3961 cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa
     4021 tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc
     4081 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
     4141 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
     4201 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
     4261 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
     4321 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
     4381 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc
     4441 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
     4501 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
     4561 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa
     4621 cggaacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
     4681 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
     4741 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgagacggtt
     4801 ataagccatt accgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc
     4861 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
     4921 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
     4981 cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg
     5041 catagcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
     5101 gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg
     5161 tttcccctga ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
     5221 aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg gttcgctgga taataatggc
     5281 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
     5341 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
     5401 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
     5461 tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca
     5521 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa
     5581 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga
     5641 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
     5701 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga
     5761 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
     5821 tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg cgcgtaaaaa
     5881 tttatgggcc tagagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga
     5941 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa
     6001 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca
     6061 gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc
     6121 aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct
     6181 tgtccattac cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc
     6241 tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag
     6301 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
     6361 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
     6421 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
     6481 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
     6541 ggcatcacaa acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg
     6601 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
     6661 agcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
     6721 agccatgcgc cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg
     6781 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
     6841 gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc
     6901 agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
     6961 ctcaccacgc ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag
     7021 tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct
     7081 acaccaatta ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
     7141 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
     7201 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
     7261 ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag
     7321 tgtggtgcaa tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
     7381 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
     7441 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
     7501 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
     7561 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
     7621 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
     7681 gaattcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
     7741 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
     7801 cgcagaagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg
     7861 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc
     7921 gctacacgcg ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc
     7981 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg
     8041 aactggtcac gaaccgtctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
     8101 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata
     8161 accggggtat ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca
     8221 aatttgttcg tctcctcttc ctgatcagga atgccgaaca aagtaaaggc agcaggcgca
     8281 ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt
     8341 tcgtagccgg attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg
     8401 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
     8461 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg
     8521 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
     8581 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga
     8641 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
     8701 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
     8761 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
     8821 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
     8881 gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
     8941 ccggagagga cgtagaccgt ttccataatg gccaggagga acgccatacc gagcgtcagt
     9001 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
     9061 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
     9121 aaagggtcac acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga
     9181 atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat
     9241 cccacgtata tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt
     9301 tgcatttttc gacggtgatc aatttatagc taaattgccg cctttcagcc aatttgatcg
     9361 agaacaattt atctcttttt gatgcccatt tccaagactt atacattgat aaatatcaat
     9421 ttctacaaat tatgttaatc atgtttcaac aaggtaactt tagtgatatt tatgtttaaa
     9481 atgtgtgaat tataagactg aaattagttt cggttggcaa ttaaagataa aaagagacga
     9541 agcaaaaaca gcacggttga ccttaattct ttttttggtc actggctaat acgacgcaga
     9601 gtttcaggat tattaataaa ggcataaatc tccctcccgc cgatattaac atttaattaa
     9661 cttataatta tattataatt aactcacccg tggttattat tttgttaaaa ataaagccac
     9721 atatacgtag cttgagagtg aagataaaca caaaattacc tgatgcgcta cgcttgccag
     9781 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
     9841 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
     9901 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
     9961 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10021 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10081 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10141 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10201 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10261 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10321 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10381 ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt ccccagtaag cccacgccgc
    10441 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtagcc
    10501 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag ttgagcatat
    10561 tccataccgg aaccggcgct tctttccagc tttcttgctg ccagttgttc attgcactgt
    10621 cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta tcggtgttaa
    10681 aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    10741 ccacatcaat gcgcctgttg ttatgactaa gagtgacaac caactcgaca tctactctgc
    10801 cagtggattg tcttgcgctg cgttgttgca gattacgcgg caccgccatt tcatagcgga
    10861 taacagccct gctctgccag gcctcatgaa taatctcgca ttgcggtttc gcattcgccg
    10921 aagtaattac ccactcttct tttgcgggtg aatagtcata ttcatcacca tcatcagagc
    10981 tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct accagttgta
    11041 gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc ccttcagcgt
    11101 cactttttgc cgcaatcacg ttgccaggct gattcgcttc gatataaagc gtgcgatagc
    11161 ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga tcgtaattac
    11221 cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca tggcgaataa
    11281 aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg cgcagccgca
    11341 cagtggtgtt gatcacttct tcccgcggcc agggcatcag gttaaacagg acgagtttgt
    11401 cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat atagaaacgc agcagattat
    11461 ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc gcgatgaact ttgtcacgac
    11521 agcagcagcc gatgctgtcg tgggcatgat tttttaagat ctctttccac attttctcca
    11581 gcaagccgtg gtggtatcca aaacccaacg tcctggccag cgttgccagc ggttccagca
    11641 gattaacaat cttattttca atacgtgcgt gggcaacttt gatatccatg cgcgtggaac
    11701 cgatggtgcg atgcacgcgc atatatttgc catcaataaa ttcccctttc agggttgcca
    11761 gattatctcg ctgcgcttcg atcttctcaa atacctcttc aaagcggctc atcacaaact
    11821 ttcgttgagg ataaatttcg cgcagtttct ccatcacttc gaagatattt tgctgcaatg
    11881 gcatctgatc atgcccattc ggcagcaaaa tctctttggt tacagacgct ttttccagca
    11941 cgtcaaaata actgtcgagg cgtttgcgta atccgttttc gtccgcaggt aagtacttac
    12001 cgatggcata acccaacggc atcacctgcg ccgtcacttc gctaccgtca ctgctttgcc
    12061 acaaaaactc ggttttatcc gtaccgtggc gctcagaaca tccacgccag aacatggtgc
    12121 aggtaatgcc aaatccattg tagatatgcg gcagttgccc ggacatgcca aaggaatcgg
    12181 gtaaataacc tattttcatc ggctcgccaa acgcgaggca gtcacgcatt ccgtacatca
    12241 gattacggac gatggattcc gcagaaacaa tcgtggtatc ggtctgggta taccaggggc
    12301 cgataatcaa cttgccggct tctaccagtt tcttcaagcg gtctttgttt tccggtttca
    12361 ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag tacgtaatat ttgtattcgt
    12421 tgtcctgttc cagtcggcac aggatctctt ccatattatt gaccagcaga atgcgtgact
    12481 cttcggtggt gaaataccac tcccgatccc agtgcatatg cggggtgatg tgaacgcgag
    12541 ataccgcttt catcgtcgtt tcctttttgt ttatggcatt acgccatcag tcagatagtt
    12601 gccatgctta accgcgtgac gccgccagat caggagaatt gcagtcgaga ttgcagcccc
    12661 caccaatgcc gcaccaaacc agccaattgc cgccataaca ccgcccgcgc cattatcatg
    12721 aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa aggccgatat tcatcgcacc
    12781 gacaatagcg cccgttacca tagagcccag cacaaacgaa ccaataaccc gcagcggatc
    12841 ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc agccctaaca gccaggtgga
    12901 tttcccggtc tcaatttcaa actctttaaa caggcgcggt gcgagcatcg tggaagcggt
    12961 tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg gcatacgggc cgtaaacgcc
    13021 gttcgccatt gcgcccaggc agaatgcata agcggcttta ttcactggcc ctccaaggtc
    13081 aaaggaacac ataaaaccga gaatggcacc cagcaacagc gcgttacttc ctgacagacc
    13141 gttcagccag gcggtaagcg agttattgat ccaggcgaca ggttccccca ccacaaacag
    13201 catcagactg cccgctccca atgtaccgag caccgggtag agataaaaag tcaggaagcc
    13261 attgaattta ctgctaagac gcaagtgatt tttcatccag cgtatcaagt aaccggctat
    13321 caatccgcca acgaccgcgc cgagaaaccc ggagccgatc atgttggcgg caagtccagc
    13381 cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtaaggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acaggcacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgagggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcact taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca ggttcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgacaaaaca ataccattgc gtaatcaatt tcacttgatt gaaattattt
    14581 gcattttatt tgtattgttt acaggatgag tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtttggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaagg ccatcatacg atggccaacc atgtcgaaac
    15421 cgacagccct tcgccattcc tgtagaggga agcggcgagg gctatttctt attacagata
    15481 tttatttcag aacagttttc agtgcttcac cgatatccgc caggctgcga acggttttca
    15541 cgcctgcggc ttccagagca gcgaatttct catccgcagt ccctttccca ccggcaatga
    15601 tcgcacccgc gtggcccata cgtttgcctt tcggcgcagt cacaccagcg atgtaaccca
    15661 caactggctt ggtaacatgc tctttgatgt acgctgccgc ttcttcttca gcgctaccgc
    15721 cgatctcacc gatcatcacg atcgcttcgg tctgcggatc tttttcgaac atttcgagaa
    15781 tgtcgataaa gttagagcca gggatcgggt caccgccgat accgacacag gtcgactgac
    15841 cgaaaccgta atccgtggtc tgtttaaccg cttcataggt cagtgtaccg gaacgggaaa
    15901 cgatacccac tttacccggt ttgtgaatgt gacccggctg gataccaatt ttgcattcac
    15961 ccggggtgat aacgcctggg cagttcgggc cgatcatacg aacgcctgct tcgtccagtt
    16021 tcactttcac ggtcagcata tccagcgtcg ggatgccttc agtgatggtg ataatcagtt
    16081 tgatgcctgc atcgatagct tccagaatgg agtctttgca gaacggtgct ggtacgtaaa
    16141 taacagacgc ggaagcgcca gtagcagcaa cagcttcacg cacggtgttg aacaccggca
    16201 ggccgaggtg ggtagtgccg cctttacctg gagttacgcc accaaccatt ttagtgccgt
    16261 atgcaatggc ctgttctgag tggaaagtcc cctggctacc ggtaaagccc tggcagataa
    16321 ccttggtgtt tttatcaatt aaaatggaca ttatttcccc tccactgcgg caacaacctg
    16381 ctgagctgca tccgtcagac cttttgctgc aataatattc aggccgctgt cagccagttt
    16441 cttcgcgccg agttcggcgt tgttaccttc cagacgtacc acgaccggta cgttaacacc
    16501 cacttccgct accgcgccga tgataccgtc agcgatcagg tcgcaacgaa cgataccgcc
    16561 gaagatgtta accagaacgg ctttcacttt gtcgtcagag aggatgattt tgaacgcttc
    16621 ggttacacgt tctttggttg cgccgccgcc aacgtccagg aagttagccg gttcgccgcc
    16681 gtgcagttta actatgtcca tcgtacccat cgccagacct gcgccgttaa ccatacaacc
    16741 gatgttaccg tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg cttcacgcgg
    16801 atcttcctgc gactggtcac gcatttcgcg cagatcaggc tggcggaaca gggcgttgcc
    16861 atcagcgccc agtttgccgt cgaggcaaat cagatcgccc tgtttggtga tgaccagcgg
    16921 gttgatttcg atcagcgcca ggtcgcgctc caggaaaatg gtcgccaggc ccatgaagat
    16981 tttggtgaac tgctgaacca gtttaccttc cagacccagt ttgaacgcca gctcgcgtcc
    17041 ctgatacggc atcgggccag tcagcggatc aagcgcaact ttatggatca ggtgcggagt
    17101 ttcttccgcc actttttcga tttccacgcc gccttcggtg gaggccataa agaccacgcg
    17161 acgggaacta cggtctacaa cggcaccgag atacagctct ttagcgatat cggtcgccgc
    17221 ttcaaccaga atctggttaa ccggttggcc attggcatct gtttgatacg ttaccagacg
    17281 cttgcccagc cagttttctg caaaagcacg gatgtcttct ttgctgttta caactttcac
    17341 accgcccgct ttaccgcggc caccagcgtg aacctgacat ttcactaccc acggaccggc
    17401 accgattttt gaagcggctt cttctgcttc gcgcggagta gtacaggcat aacccaccgg
    17461 tgctggtaag ccatagcggg caaacagttg ttttgcctga tattcatgta agttcatgtg
    17521 ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta gaccgacacc ctatccacgt
    17581 cggtctacag tgcaggtgaa gcttaaacta ctacacgtcc agcagcagac gcgtcggctc
    17641 ttccagtaac tctttgatcg ttaccaggaa gcccacggat tcgcgaccat cgatcagacg
    17701 gtgatcgtag gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcaccgc
    17761 catcggacga tctttgatag cgtgcatacc cagaattgcg ctctgcggcg ggttgatgat
    17821 cggcgtagac atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt
    17881 cagatcttca accgtcagct tgccatcacg gcctttgact gccagctctt tgattttctt
    17941 ctcgatgtct gccatgccga gggtatcgac atcacgcaga accggcgtca ccaggccgcg
    18001 cggcgtagaa accgccatgc tgacgtcgaa atagttgtgg taaaccacgt catcgccgtc
    18061 gatagacgcg ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa
    18121 ggacataaag cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg
    18181 cagatccata atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt
    18241 tttcgcttcc agcagacgct ctgccacacg cttacgcagg cgagtcatcg ggacacgttt
    18301 ttcactacgt gcagccagag ccggttgcgc cgccggagca gccgctgccg gagcagactc
    18361 tttcgccggg gctttcgcca gatgtttttc cacatcttca cgagtcagac gaccacccac
    18421 accggtgcct ttaatggcgc tggcgtcgag attgtgttca gccagcaggc gacggatcgc
    18481 cgggcttaac gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt
    18541 ggacgctttc tcttcagatt tggcgctggt ttctttaccg gcgctgttgc cttcacgcag
    18601 gcgaccaagg atctgacgag acgttaccgt tgtaccttca tcttccagaa ccgcatccag
    18661 aatgccgtct gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag
    18721 cacttcatca cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc
    18781 ggctacggat tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt
    18841 atttattcga cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac
    18901 atatacccta ccgccggaga ggcggaggcc gggcggcctg cataacgcag agaagcccca
    18961 aacggaatca cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc
    19021 tcttcctggc accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc
    19081 gctttatgcg ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg
    19141 ttcttacgac gctgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc
    19201 ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc
    19261 gccagttctt cgaggctgga aaccgccagc ggatgacgca gcagggattt cggcgacatc
    19321 acgaccagcg gacgacgcat cccgcgcagc gcctgacgac gcagcatgtg gtaaacctgt
    19381 gccggggtag acggtacgca aacctgcatg ttttgctcag cacaaagttg cagataacgt
    19441 tccagacgcg cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc
    19501 accagaccac acatccggcc ccatttctgc tcgccagagg agatgaactg gtcgataacc
    19561 acctgcgcac cgttggcgaa gtcaccgaac tgcgcttccc agatggtcag ggtgcgtggt
    19621 tctgcggtgg cataaccata ttcaaacgcc agcacggctt cttctgacag tacggagtcc
    19681 cagacacgga acgcgccctg accgttatgg atgtgttgca gcggcgtgta agtggaaccg
    19741 ttagactggt tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca
    19801 cccgacaggc gaaccggaat gccttcatca accagcgtgg cgtaagcgag gttttccgca
    19861 ccgccccagt cgaacagttt ctcaccggct gccatcgcct ggcgatcgcc ataaatcttg
    19921 gcaacgcgag actgcatttc aactgcttct ggtaccgtac tgatacgttt agccagttcc
    19981 tgcaggcgct tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac
    20041 ggcgaccagg tgaaagagtg catgttcatc ggacgccact ctgccactac gcaatcgcca
    20101 gcatccagcg catcgcggta caggttaacc atctcggtgg catcttccag cgtcgccact
    20161 ttttcctgct ccagcttgtc agcgtagatt ttgcgcggtg tcggatgttt tttgattttc
    20221 tgatacatca gtggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg
    20281 tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga
    20341 cgggtcacaa aggcaacggc ttccggatca tccgcgttaa cgtggaaaat cggtgcctga
    20401 accatcttac cgatatcagt acagtacggt gtagaacggg catccagcgg gttagaggtg
    20461 gtgaaaccaa cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc
    20521 gctttcgaca tgttcagagt ttcctgaacc acgccctgcc cggtcactgc ggcgtcaccg
    20581 tggatggtga ttggcagcac tttgttgctg ttcggctcat caagtctgtc cagacgggca
    20641 cgaacagagc cgataacgac cgggcttaca atctcaaggt gagacgggtt aaacgccagc
    20701 gccaggtgca ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc
    20761 acgtcgcctg tgccgaggtg ttctttatgt ttaccggcga actcgtcgaa caagtcttgc
    20821 ggttttttac ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga
    20881 accacttcgc gggtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt
    20941 aacgcgtcac cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccgaggtaa
    21001 cgctcaaggc cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg
    21061 aaagtcgcgc gaccagactc gatacgctgt tggatccagc gtttttcttc ggtgctggtg
    21121 atgtgcatat actcggcacc aatcgggccg cagtaggttt gcttgagggc ggaaataagc
    21181 tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtttcctgg
    21241 aagtctgctt cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc
    21301 cacagtccca gcggatctag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg
    21361 agctgcagga ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa
    21421 cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc
    21481 ggtttgactc ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag
    21541 tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag
    21601 aggtaagaag agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca
    21661 tcttttttat gcttacttcg ccgtggatac taccacgcac agtgatgtgc gtgccggggt
    21721 tcacttgcga gctcttgtct acgtagtggc tcgcgaagga acctttaaaa actgtcttag
    21781 tgtcaggggc agtttttaaa gatttcctgc atttctttgt tacgcctgat gcgctgcgct
    21841 tatcaggcct gcgggtaatg acttgtaggc cggataaggc gttcacgccg catccggcac
    21901 tggttgcctg atgcaacgct tgcgcgtctt atcaggccta cagtttacgc attacgttgc
    21961 aacaacatcg acttgatatg gccgatggcg cgcgtcgggt tcagcccctt cggacataca
    22021 ctgacgcagt tcatgatgct gtgacagcgg aatacgctga atgcatcgct caaaccgtcg
    22081 aggcggctgt cagtctcggt atcacggcta tcgatcagga aacgatatgc cgctaacaag
    22141 cctgccgggc cgataaactt atcgggattc caccagaaag acggacaaga ggttgaacaa
    22201 catgcgcaga gaatacattc atacagcccg tcgagttttt cgcgctgctc tggcatctgt
    22261 aaatgctcgc gagctggcgg attttgtcca ttattcaaca ggtaaggctt aattttctca
    22321 tattgcgcat agaattgtcc catgtctacc accaaatcgc ggatcaccgg taaacctggc
    22381 agcgggcgaa tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg ggtaatacag
    22441 gccagaccat tcttgccgtt catgttcaga ccgtcggaac cgcacacacc ttcacggcag
    22501 gagcggcgga acgacaggct gggatctttc tctttcagct ggataagcgc atccagcagc
    22561 atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg agcatcatca
    22621 acatccgggt tatagcgata aattgaaaac tcgagtctca ttttcctgtc tccgcattag
    22681 taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt gacgcttcgg
    22741 cgcgtcatgg attccgactc aggcagatac agggagtggc acagccagtt ttcatcatca
    22801 cgatccggga agtcgaagcg gctatgtgcg ccacggcttt cggtacggaa gttggcagaa
    22861 acagccgttg catacgccgt ttccatcagg ttatccagtt ccaggcactc aacgcgctgg
    22921 gtgttgaact cgctggaagt gtcatccaga cgggcatttt tcagacgctc gcggatcact
    22981 ttcaactgct caagcccttt cgccatcgca tcaccttcac ggaagaccga gaagttatgc
    23041 tgcatacatt cttgcagcgc tttacggatc gccaccggat cttcaccgtt acgattattg
    23101 ttccaccggt tcaggcgatc cagagacgct tcaacatcag actcgctggc atcgtgcagt
    23161 gcgccctgct cggcgataga ctcttgcaga tgcagacctg ccgcgcgacc aaagaccacc
    23221 aggtccagca gcgagttgcc gcccagacgg ttagcgccgt gtaccgatac acaagcgatt
    23281 tcaccaacgg caaacagtcc cggaacaacc acatcttcgc ctttctcatt cacagtcagc
    23341 gcctgaccgg taactttggt cggaataccg cccatcatgt agtgacaggt tgggataacc
    23401 ggaatcggct ctttcaccgg atcgacgtga gcgaaggtac gggaaagctc caggataccc
    23461 ggcagacggg attcgagaac ttctttaccc aggtgatcga gtttcagttt cgcgtgtggc
    23521 ccccacggac catcacagcc gcgaccttca cggatttcaa tcatgatgga acgcgccacc
    23581 acatcacggc ccgccaggtc tttggcgttc ggcgcataac gctccataaa gcgttcgcca
    23641 tgtttgttca gcagataacc gccttcacca cggcaacctt cggtgaccag tacgcccgca
    23701 ccggcaatgc cggtcgggtg gaattgccac atttccatat cctgcaccgg tacgcctgcg
    23761 cggatagcca tgccgacacc gtcgccagtg ttaatgtggg cgttagtggt ggactgataa
    23821 atacgccctg ctccgccagt cgccagcacg gtagcgcggg ctttgaaata aaccacttca
    23881 ccggtttcga tgcacagtgc ggtacaaccc accaccgcgc catcctggtt tttcaccaga
    23941 tccagcgcat accactcgga gaaaatggtg gtgtggtttt tcaggttctg ctgataaagc
    24001 gtgtgcaaca gtgcgtgacc ggtacggtca gcagccgccg cagtgcgtgc cgcctgctcg
    24061 ccgccgaagt ttttcgactg accgccaaac ggacgttgat agatacgacc atcatcgaga
    24121 cgcgagaacg gcaggcccat gtgttcgagt tccagaatcg cttccggccc ggttttacac
    24181 atatattcaa tcgcgtcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata
    24241 tgccattccc agttatcttc atgggtatta cccagcgcaa cggtaatgcc gccttgcgca
    24301 gaaacggtat gggaacgggt cgggaagact ttagagagca gggcacaggt ctggccgctc
    24361 tgggaaattt gcagcgccgc gcgcatacct gcgccaccgg caccaatcac aactgcatca
    24421 aattctctga ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt
    24481 aaaccaccag tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc agcggtttaa
    24541 cgtagtcggt caacacctgc cacatgccga tccaggcatg aatcaagata gaaaacagcg
    24601 ccagcagggt gaacactttg gtgaacgcag aggcgaagaa accgatccag acttcatatg
    24661 tcagttcgcc actggtggcg aaaaaaccga ccatataaat gatgtagagc gtcaggacga
    24721 tagcggtagc gcgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcggaggcgt
    24781 tgcttaccat acgaggactc ctgcgagaag tgaaagcacg acagtaataa caaaggagat
    24841 tttggcggag cgtttacccg cttcgaatgt ttcttccaga tagccaaaat ccatcatcat
    24901 gtggcgaata cctacgacga cgtgatacgc cagagcggta aggatgcccc acatgataaa
    24961 tttgacgaag aagctgccca taatcgcgga agcttgctcg aaaccttcag gggaagagag
    25021 gctggtaccc agaagccact gcaggatgcc cactgcaaca aaggtgatca caccggaaac
    25081 gcgatggaga atggacgcta tcgccgtgat ggggaaccgg atggtctgta ggtccagatt
    25141 aacaggtctt tgttttttca catttcttat catgaataac gcccacatgc tgttcttatt
    25201 attccctggg gactacgggc acagaggtta actttctgtt acctggagac gtcgggattt
    25261 ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc cataactgcg cgtcatgcaa
    25321 aacactgctt ccagatgcga aaacgacacg ttacaacgct gggtggctcg ggattgcagg
    25381 gtgttccgga gacctggcgg cagtataggc tgttcacaaa atcattacaa ttaacctaca
    25441 tatagtttgt cgggttttat cctgaacagt gatccaggtc acgataacaa catttattta
    25501 atttttaatc atctaatttg acaatcattc aacaaagttg ttacaaacat taccaggaaa
    25561 agcatataat gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca
    25621 gtgtggaagt attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat
    25681 tcggattgct aagtacttga ttcgccattt attcgtcatc aatggattct ttacctgcaa
    25741 gcgcccagag ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca
    25801 aacgggtaaa gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgcaat
    25861 aaggcgctaa ggagacctta aatggctgat acaaaagcaa aactcaacct caacggggac
    25921 acagctgttg aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt
    25981 actctcggtt caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgc
    26041 gaatctaaaa ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg
    26101 atcgatcagc tggcgaccga ttctaactac ctagaagttt gttacatcct gctgaatggt
    26161 gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg
    26221 atccacgagc agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca
    26281 gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac
    26341 aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc
    26401 gcgatgtgtt acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc
    26461 tatgccggta acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat
    26521 ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac
    26581 gcctctacct ccaccgtgcg taccgctggc tcttcggggg cgaacccgtt tgcctgtatc
    26641 gcagcaggta ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg
    26701 aaaatgctgg aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa
    26761 gataaaaatg attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac
    26821 ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa
    26881 gatgacctgc tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt
    26941 atcgagaaga aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg
    27001 ggtattccgt cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc
    27061 gcccactgga gcgaaatgca cagttacggt atgaagattg cccgtccgcg tcagctgtat
    27121 acaggatatg aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt gattgctaag
    27181 ttgtaaatat tttaacccgc cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa
    27241 aaaattgtaa aataaaatcc actatcggac attcatatat tctaaaaaaa aatacatcgg
    27301 tataaattat aaacaaatag atgattttta atcatctatc ttcctctcag ttacatgaat
    27361 aaaccaccaa atatagcgca cccctataat tcccaatact ttattcatca aacatttaat
    27421 tcataccatc aattaattac ttttacattt aataaaatta ataatatttt taagaaaaac
    27481 taaaaacatt cgacaatcaa ataagatatt cttgcaccga tatacttttt tcttttctgt
    27541 taaaggactc agttatgttt aaaggacaaa aaacattggc cgcactggcc gtatctctgc
    27601 tgttcacagc acctgtttat gcggctgatg aaggatccgg tgaaattcac tttaaaggtg
    27661 aagttattga agcaccgtgt gaaatacatc aggatgatat tgataaagag gttgaactcg
    27721 gtcaggtgac caccagccac attaatcagt cacatcacag cgatgccgtt gctgtcgacc
    27781 tgctcttagt caactgtgat ctggaaaact ccagcaacgg ttccggtggc aagatttcta
    27841 aagttgcagt cacctttgat agctcagcga aaaccaccgg cgcagatccg attctcaaca
    27901 acaccagcac gggtgaagcc accggcgtcg gcgtacgttt aatgaataaa gatcaaagca
    27961 atatcgtttt aggcaccgct actccagata tcgacctggc tccgacctcc agcgaacaaa
    28021 cgctgaattt ctttgcctgg atggaacaaa ttgatcaggc tacacctgta acgccaggcg
    28081 cagttacggc gaatgcaacc tacgtgctgg attataaata agtattactt tggcggcgct
    28141 atcgccgcct ttcataaaat atttatcagg tatggacacc gtgaatattt atcgactctc
    28201 ttttgtatcc tgcctggtcg tggcgatgcc ttgcgcattg gcggtcgaat tcaatctgaa
    28261 tgttctcgat aaatcaatgc gcgaccgcat tgatatttca ttattaaagg aaaaaggagt
    28321 cattgctccc ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa tcagtaacga
    28381 gcaaaagatt aactggcaca aaaatgacga taaaaccatt ccgtgcatca atgatttact
    28441 ggtcgataaa tttggcttaa aacctgaagt ccgtcagtcg ttaccattga taaatcagtg
    28501 cgtcgatttt agctcccgac ctgaaatgct cttcaatttc gatcaagcca atcagcaact
    28561 gaatattagt attccgcaag cctggctggc gtggcactca gaaaactgga ccccaccctc
    28621 cacatggaaa gaaggtgtcg ccggtgtcct gatggattac aacttgtttg ccagcagcta
    28681 ccgcccacag gatggcagca gcagcactaa tcttaacgcc tatggaacag ccggaatcaa
    28741 cacgggggca tggcgcttac gcagtgatta tcagttgaat catactgata gcgatgataa
    28801 ccatgaacag tcaggcgaaa tatcacgtac ctatcttttt cgcccgttac cgcaattagg
    28861 ttcaaaatta accctcggcg aaacggattt tagtcccaat attttcgacg gtttttctta
    28921 taccggcgcg gcactggcaa gtgacgatcg aatgttgcca tgggaactac gcggctacgc
    28981 cccacaaatt agcggtattg cacagaccaa tgccacggtg acgatcagtc aatcaggccg
    29041 cgtcatttac cagaaaaaag tcccaccagg cccatttatc attgacgacc ttaatcagtc
    29101 tgttcagggc acactggatg tcaaagtgac ggaagaagat ggtcgggtga acaatttcca
    29161 ggtttcagca gcatcgacgc ccttcctgac tcgtcaggga caggttcgct ataaactggc
    29221 cgcgggtcag ccacggccct ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa
    29281 tgaagtttcc tgggggatgc tgtcaaacac ctcgctgtac agcggcctgc tgctttctgg
    29341 tgatgactac cattctgccg caatgggtat tgggcaaaat atgctgtggc ttggtgcgct
    29401 gtcgtttgat gtcacgtggg ccagtagcca ttttgatact cagcaggacg agcggggctt
    29461 aagctaccgt tttaattaca gcaaacaagt ggatgccact aacagcacga tttccctcgc
    29521 cgcttatcgt ttctccgatc gtcattttca cagctacgcc aactatctgg atcacaaata
    29581 caacgacagc gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat
    29641 taccccacta aacctcaatc tttacgccaa cctgctacat caaacctggt ggaatgcaga
    29701 cgcctccacg accgccaaca tcacggcagg ttttaatgtt gatattggtg actggagaga
    29761 tatctctatt tctacgtcat tcaatacaac ccattacgaa gataaagatc gcgacaacca
    29821 gatttacctg tcgatttcgc tccccttcgg taacggtggt cgggttggct atgacatgca
    29881 aaacagtagc cacagcacca cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa
    29941 tagctggggc atgtctgccg gactgcaatc cgaccgtcct gacaatggag cccaggtgag
    30001 cggtaactat cagcacctga gttcagcggg tgagtgggat atttctggta cctatgccgc
    30061 caatgattac agttccgtca gcagcagctg gagcggttct ttcaccgcaa cccaatatgg
    30121 tgcagcgttt catcgccgca gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg
    30181 cgtggcagat attccggttc agggcaatct cgactacacc aaccattttg gcattgcggt
    30241 ggtgccgttg atttccagtt accagccttc caccgtggcg gtgaacatga atgacttacc
    30301 cgacggcgta acagttgcag aaaacgttat caaagaaacg tggattgaag gcgcgatagg
    30361 ttacaaatca ctggcttccc gttccggtaa agacgttaac gtcatcattc gcaacgccag
    30421 cggtcagttt cctcccctcg gagcggatat ccgccaggat gacagcggca ttagcgtggg
    30481 gatggttggc gaggaaggac atgcctggtt aagcggagtc gctgaaaatc aaaagtttac
    30541 cgtggtctgg ggtgatagcc agcattgctc gctccatctt cctgaacata tggaagacac
    30601 cgcaaatcgc ctgattttac cttgtcatta attaaggaaa tgaaaatgac atttatgaaa
    30661 ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact acaacccgat
    30721 cgcactcgta ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt ggataatcgt
    30781 agcgataaac tcccctacct cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc
    30841 gatgatcttc tggtcgccct gccgccgata caacgtctgg aaccgaaagc aacaacacag
    30901 gtacggatag taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga aacgctattt
    30961 ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg
    31021 caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa
    31081 gcaggagaaa aggtcgaact gcagttacag gtcagccagc agggcaacca actgacactg
    31141 aaaaatccta ccgcgtatta tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt
    31201 ctccccggct tcaaaaccgt gatggttgcg ccttttagta cggtcaacac aaacaccgga
    31261 aattatagcg gcagccagtt ttatctcggt tatatggatg attacggcgc gttacgcatg
    31321 acgacgctca actgcagcgg acaatgccat ttacaagccg tggaggcgaa gaaatgagtg
    31381 ctggcaaggg agtgttgctc gtcatctgtc tgttatttct gtcgttgaag tctgcgcttg
    31441 ccctgaactg ctactttggt tcatccggtg gctcggtaga aaaatcagaa gcgattcaac
    31501 cttttgctgt accaggcaat gccaaactcg gcgataagat ctgggaatct gacgatatta
    31561 aaataagatc tgggaatctg acgatattaa aattcccgtc tattgtgaca acaataccaa
    31621 cggcaacttt gaaagcgaac atgtctacgt atgggtaaac ccctacccgg gggtgcagga
    31681 tcgttattat caactcggag taacttacaa tggcgttgat tatgacgcca accagggaaa
    31741 aagccgcatc gacactaacc agtgtatcga cagtaagaac attgacattt atacccctga
    31801 tgggatggca gaacaaaatt tgctcgggtg atcccgctaa tattcacatg tcgcgaacct
    31861 ttctcgctcg catgcggtta tacgtcaaaa tacgagaaat gccgccgcat gattatcaaa
    31921 gtacgcttag cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt aacgaagacc
    31981 ctactgccca aaacctgaaa tatcatatta ctggtctgga aaacattcgc gtgctggatt
    32041 gcagcgtcaa tttttccatt tcaccggaaa cacagatgat tgattttggt aaatttaatt
    32101 tgctggatat tcgtcgccac acaatgtcga aaacattcag tattaagacg acaaaatcac
    32161 aaaatgacca atgcaccgac ggatttaaag tcagttcctc cttttatacc gaagaaacgt
    32221 tggttgaaga agataaagca ttgttgattg gtaatggtct gaagctacgt ttgctggatg
    32281 aaaacgcctc gccttatacc ttcaataaat atgctgaata cgccgatttc accagcgaca
    32341 tgttggtcta cgaaaaaacc tatacagctg aactttcgtc cattgcaggt acccccatcg
    32401 aggctgggcc cttcgataca gtggtgcttt ttaaaattaa ctataactga tttgccatga
    32461 tgccctttgc agccggaaag ggcatctctt tcagaaaatt gctgattttc cccttttcct
    32521 tgattgctgt tattttttcc tagcccggct ttctttatgc tgtatcgata caccaaagag
    32581 aaaataatga gggagcgaag gatgccagtt ttgcagtggg gaatgttatg tgtgctatca
    32641 cttctccttt ctattggttt cctcgcggta catctcccgg cggcgctatt acttgggccg
    32701 atgatcgccg ggatcatctt cagtatgcgc ggaattactc tgcaacttcc ccgctccgct
    32761 tttctcgccg cgcaggccat tcttggctgc atgattgcgc aaaacctcac tggttcgatt
    32821 ctcaccacgc tggccgccaa ctggccgatc gtgcttgcta ttttactggt gacgctgctt
    32881 tccagcgcca tcgtgggttg gttattggtg cgctatagct cactgcctgg aaataccggt
    32941 gcctggggtt cttctccagg cggcgcagcg gcaatggtcg ctatggcgca agattacggc
    33001 gcagatattc gcctggtggc gtttatgcaa tatctgcgag tgctgttcgt cgcgggggcc
    33061 gctgttctgg tcacccgcat gatgctgggt gataacgctg aagcggttaa ccagcaaatc
    33121 gtctggttcc cgccagtgag cattaatctc ctgcttacga ttttgctcgc agtcgttgcc
    33181 ggtacggcgg gatgcatgct gcgtcttcct tccggcacga tgctcatccc gatgctggcg
    33241 ggcgcagtgc tccagtctgg tcagctcatc accatcgaac taccggaatg gctgctggcg
    33301 atggcgtata tggcaattgg ctggcggatt ggtcttggtt tcgataaaca aatcttactg
    33361 cgggcactac gcccgctacc gcaaatcctg ctgtcgattt ttgctctgct ggctatttgt
    33421 gcaggtatgg cgtggggact gacccggttt atgcatattg attttatgac tgcctacctc
    33481 gccaccagtc ccggcgggct tgataccgtg gcggtcatcg ccgcagggag caatgccgat
    33541 atggcgctta ttatggcgat gcaaaccctg cgcctgttca gtattttgct gacggggcca
    33601 gccattgcgc ggtttatttc aacctatgcg ccgaagcggt cggcctagtg ctggcagcct
    33661 ggacaccagt aaaacgggcg cgaggacaac gtggttttct caatgatgcc gccacagcgt
    33721 tcgcacagtt cgccatcacg atgaaaaacc ttaaagcgaa acagcgcccc atgatgctta
    33781 ttctcatcca cctgcccccg cgtagcgtaa gaaaaccgag gaatctccag taacgcgtgt
    33841 gcgagtgcat ccagttgcgc cgcattgaga tctttcgctt tatgatttcc agtcaaccca
    33901 acctgccaga ggatctccac ccgcaaataa ttgccaagcc cagccagaaa cgcctgatcg
    33961 agcagtaatc ctgcaaactg acggttacga aagcgcggcg acaataaccg ttcctttacc
    34021 acctccggcg tcagattcgg atccagcaca tcgggaccaa cgcgttgtaa aaacggatgc
    34081 gtggtcagtt gttccggcct caacatctca atatccgagg cgctataaag cagaatggtt
    34141 ttgtcagccg tttgcagttt tacccgcaat actcgcgtgg tctgcggctc ttcgccggta
    34201 tcaaccacgc gccagacgcc gtaaagctga ttatggctgt agagcgttaa accgttggga
    34261 aaatgagtta acaacgcctt accacgcgtt tccacatggg taacgtgttg accgataagt
    34321 tgtgattgat aagtttttaa ctgcgggaag gcaaaccaga catcagttag tggtttgcct
    34381 ttgatcgccg cctccaggtt atccgctgca cggcggatct ccgggccttc aggcatggtg
    34441 atatcctttt ttcattcttt attaagcggg gttatgctgc gacaacaatc ccctttttgg
    34501 caaatgtagc tcgtaatcgg cgggcgaacg ccagagcgtg ctcgccgtcg ccatgcaggc
    34561 agaccgtttg cgccgcgacc gttgcccatt cgccggtgat acttttgact ctgccgtgtt
    34621 gcaccatctc cagcgtttgc gccaacgcct gttcttcgtt ttcaatcaac gcgcctggct
    34681 gacttcgcgg caccagcgag ccgtcagcct gataaccgcg atcggcaaac acttcctcgc
    34741 gcgttgtcag gccatattgc ttgcctgcac gaatcagctc gcttcctgcc agcccaacga
    34801 gaaccaatgc cggatcgcaa gcgtataccg ctctggcgat ggcgtctgcc agttgtgctt
    34861 ctttcgccgc ctggttgtac aacatgccgt gcggtttgac atgacgcatt acgccgcctt
    34921 gcgcacgggc aatcgttgcc agcgcgccaa tttgatacag cgtctgggcg taaacggttt
    34981 ccggcggcag ctgcatggcg ctgcgaccaa aattttccct gtcgggaaaa ctcgggtgag
    35041 cgccaatcgc gacaccattt tttattgctt cacgcacgca agcctgcatg gtttgcgcat
    35101 cgcctgcatg aaatccacaa gcaatattgg cagaggaaac cagcgttaat agctctgcgt
    35161 cgctggcgca gccttcgccc agatcggcgt tcaggtcaat tttcattgtg cagccgccac
    35221 gctaattgtt caaaataacg ttgctgatct tgccgcgctt tcagtgcctc ttccagtgaa
    35281 cactggacaa aatgaatcgg ctgaccgagc ggaatttgcg ccagatggta catatcagcc
    35341 tcaatgatac aggcaatacg tggataacca ccggtggtct gtgcgtcgtt catcaacaca
    35401 atcggctgcc cgttatgcgg cacctgcacc acgcccggta acaaaccgtg agataacagt
    35461 tcgcgatcgg tgatgcgttt taaaatttgc ccctgtaaac gatagcccat tcggttactt
    35521 tgcgagctaa gctgccaggg cgaacgccag aatgcatcct gcgaggcgcg atcgaactca
    35581 tgatattccg gccccggcaa ggcgcgaatg cggttgcccc acagcagctg tttaacgccc
    35641 tgcgcttcca taaaatcacg cttcgatttg ccaatcggga gtcagtcacc atccttcagt
    35701 aaacggcctt ccagcccgcc aatccccact ttgagatcgg tgctgcatga ccccattacc
    35761 ggcggaacat caataccacc cgcgaccgcc agataactgc gcatcccgtg ctgcgggcgt
    35821 ttaagcgtta aacgctggcc tgctttcatc ggcaatcgcc agccggtcca gacggcgtta
    35881 tcatccaacc gcgcttcgca acctgcaccc gtcagggcaa accacccatc agtttcgaac
    35941 tcaactgtta actgaccgag cgtgatctcc agcgcggggg cattggcgtc attacccacc
    36001 agcaggttag caatgcgcag cgcaggcata tccagtgcgc cacagtggct gatacccgac
    36061 tggcaaaaac cgtgacgacc gccatcctgc acagtggtgt acatgcccgc acgaataatc
    36121 ttcagcatat tccctccttc tgcggtacaa atcgcacgct gtctcccgga cgtaataaga
    36181 tgggttcgtc acgcgccgga tcaaacaggc tgagtgaggt atgaccaatc aactgccagc
    36241 cacccggcgt tgccagcgga taaacaccag tctgcggccc gccgatcccg acagaacctg
    36301 ccggaacgag caagcgcggt tcagcgcgcc gtggcgtgtg tagttgttcc ggcaaactcc
    36361 cgagatacgg gaagcccggt tgaaaaccta aaaaccagac cacgtattcc acggaggagt
    36421 gcaattcaac aacctgtttt tcgctcaacc cgcaatacgc cgcgaccacc gccaaatccg
    36481 gtccgcctgc accaccgtaa accaccggaa tttcaataaa gcgagactca ggttccaggg
    36541 cctcgctctc ctcccaccag cgttgcaaac gctctatggc atccagcgcc agcgactcag
    36601 gattacgcaa aatcaccgtg atattgttca tgccgggaat ggcttcaacc acattcggca
    36661 tatccaccag acgctgcgcc agtcgccaga tccgtttctg gctagccagc gtcaccggcg
    36721 gttccagttc cagcactacc gccgtttcac ctatcagata acaacgcgct cgttgcactt
    36781 tcgatccctc tgattattcg ttatgcagga ttaggaatat caataaaggt cacatcaaga
    36841 tcggtatttt cattcagcca ctcgctcaat gcgcgaatac caccgcgttc agtggcatgg
    36901 tgacctgcag cataaaaatg caatccctgc tcgcgggctg aatgaatggt ctgttcagaa
    36961 acttcgccag taataaaagc atccacgcca aaacgcgcgg cgctatcgat aaaactttgc
    37021 ccgccgcccg tgcaccaggc gacgcgctgg accacctcag gtccggtatc gccacaccat
    37081 aacggcttac gtcccagacg cgcttcaatc caggaggcta gttccattcc cggcacaggc
    37141 atggtcagct ctccccacgg caccaacggc tcaatttcgc ccatgaccgt gatccccagt
    37201 aacgccgcca gctgtgcgtt attgcccagc tcaggatgtg cgtcaagcgg caaatgccag
    37261 ccatacaggt tgatatcatt cgccagcaac gtttttaaac ggttacgctt cattccgcga
    37321 atgaccggag actctccttt ccagaagtag ccatgatgca caatgaccgc atccgcgccc
    37381 agacgcactg cctcatcgag caaagcctgg ctggcggtga caccggtaac aattttttgc
    37441 accgtctctt tgccttccac ctgcaaacca ttcggcgcgt aatcactaat cgccgcgctg
    37501 ttcagttttt cgttaatcag ttgttccagt tcggtgtttt tcatcttcgc gccttccgta
    37561 ttctgatagc tacaccgtta acatagcgtt acatcgcctg ttcgtcgata gccaatcttt
    37621 tgacatatta agaaacaaac ccgacgccaa aagacgcaaa ttgtttatat catcacgctt
    37681 cagctaatgt atatttatgc aatcagacag catattgcat aaataatcag atctgtatca
    37741 aagttttaac tttaaggaaa cacaagcacg ccgcgcaact attacgtgac gttgccgtca
    37801 attgtggaat tattatgaat aaacacgcat cacaaccgcg cgctatttac tatgtcgttg
    37861 cgctgcaaat ctgggaatat tttagctttt acggcatgcg tgccctgctg attctctatc
    37921 tcaccaatca actaaaatac aacgatactc acgcctacga gttatttagc gcctactgtt
    37981 cgctggtgta tgtcacgcca atcctcggtg gctttttggc ggataaagtt ctcggcaatc
    38041 gcatggcggt gatgctgggg gcgttgttga tggcgatcgg tcatgtggtg ctgggtgcca
    38101 gtgagatcca tccgtcattc ctctatctgt ccctggcgat tatcgtctgc ggctatggcc
    38161 tgtttaaatc taacgtaagc tgtctgctcg gcgagctgta tgagccaacc gatccgcgtc
    38221 gtgatggcgg tttctcgctg atgtatgcgg cgggtaacgt ggggtctatt atcgcaccta
    38281 tcgcctgtgg ttacgcccag gaagagtaca gctgggcgat gggctttggc ctggcggcgg
    38341 ttggaatgat cgcgggtctg gtcattttct tatgtggcaa tcgtcatttc actcataccc
    38401 gcggcgttaa caaaaaagta ctgcgtgcga caaactttct cctgccgaac tggggatggc
    38461 tgctggttct gctggtggca acgcctgcac tgattaccgt gctgttctgg aaagagtggt
    38521 cggtatacgc cttaattgtc gcgactatta ttggcctggg tgtactggca aaaatttatc
    38581 gcaaagcaga aaaccagaaa cagcggaagg agctggggct gattgtgacg ctcaccttct
    38641 tcagtatgtt gttctgggcc ttcgcacaac agggcggtag ctcgattagc ctttatatcg
    38701 accgcttcgt taaccgcgat atgtttggtt ataccgttcc gaccgcgatg ttccagtcga
    38761 ttaatgcctt cgcagttatg ctgtgcggtg tgttcctggc gtgggtggtt aaagaaagcg
    38821 tcgcaggtaa tcgtaccgtg cgcatctggg gaaaatttgc tcttggcctt ggcctgatga
    38881 gcgccggatt ctgcattctg accttaagcg cccgctggtc cgcaatgtat ggtcactctt
    38941 ctctgccact gatggtgtta ggcctcgcgg tgatgggctt tgcggaactg tttatcgacc
    39001 cggttgccat gtcgcaaatt acgcgcattg aaattccggg cgtgaccggc gtattaaccg
    39061 gcatctacat gctgctttct ggcgcgatcg cgaactatct ggcgggcgtg attgccgatc
    39121 agacatcgca ggcttcgttt gatgcttccg gggcgatcaa ctactccatc aatgcatata
    39181 ttgaagtatt tgatcaaatt acctggggcg cactggcgtg tgtaggactg gtactgatga
    39241 tttggctgta tcaggcgctg aaattcagaa accgcgcgct ggcgctggag tcataatagg
    39301 ccagcaccga tgcctgatgc gacgcttgac gcgtcttatc aggcctacgt atttcctgca
    39361 atttattgaa tttgcacaaa tttgtaggct ggattaggcg ttcacgccgc atccggcatc
    39421 tgggctcgat tgcctgacgc gtctgttatt tccccttccg cgccgcctca tacgctgcca
    39481 acgtttgtac tctcgcctct ttgtgctcga ctatcggttg cggataatcc agcttcacac
    39541 ctgctttctg cgcccacttc cacggctcat gcaccgcttt ccctggtaca tcgcgcagct
    39601 ccggtaacca tcgacgaata aactcgccct cacggtcaaa tttctcgccc tgagttatcg
    39661 gattgaaaat acgaaaatac ggcgcagcat cagtacccgt agaagccgcc cactgccagc
    39721 caccgttatt agctgccaaa tcaccatcaa tcagctgcga catgaaatat cgctcgcctt
    39781 cgcgccagtc gatcaacaaa tctttcacca gaaaactggc tgtaatcatc cgtagccggt
    39841 tatgcatcca gccagtgctg ttaagctgac gcatagcggc atcaataatc gggtatcccg
    39901 ttttgccttt ctgccaggcc tgtaaatgtg cgggattgct ttgccactgc acacgatccg
    39961 tccaggcaat aaatggacga tgtttacaca acgaggggta atacgtcatc agatgacggt
    40021 agaactcgcg ccagatcagc tcactaagcc agacactacc ggccccaccg tccagcgcct
    40081 gcggctgttc cgccaacaag cgatgcaaac actggcgagg cgataacccg cccgttgcca
    40141 agctggcgga caaacggctg gtgccttcca ctgccggaaa atctcgttgt tgctcatact
    40201 ctccagcacc gttctcgcaa aactggcgta attgcgcaat cgccgctttt tcttccaccg
    40261 ggaaatgcgc agtatcgaaa gactgacgag gataattcag cgtaatggat ggcgcgggct
    40321 ttatcgatcc gctactacga acttttggtg cagcgacgca ctccggcatc ccttcccgca
    40381 gccgtttcag ccaggcattc ttaaaaggcg taaagacttt gtacatctcg tgattgccgg
    40441 tcatcaccgc ccccggcggc aggatcacgc tgtcatcaaa tccttcacac accacgttac
    40501 gcagcgctct ttcaacgtcc acatcccgcg ctcgctcatt cacctcatac tgatagttat
    40561 aaaaaagatg agtaacgctg ttttccgcgc acacctgttt aactatttcg acactggcga
    40621 caaagtcatc cacttcacgg aacaataaag gaataccttt ttccgccagc gcgatttgta
    40681 gcccattcag ttgagcattg ataagttcag cctgacgcgg cgacatgtta tgcgtctccc
    40741 acttgcgtgg tgtagcgata tacagcgcca gcacgcgtgc agacgaattg cggcaggcgg
    40801 cagccagtgc gagattatcg tgcagacgta aatcctggcg aaaccaggcc agatgggtag
    40861 tcataaaact cctgaatggc gcaagttaat cacacgcggc caaagttcga aataacgctg
    40921 ccctgaaagc caggcgtcag gataaagcgc catatagtgt ttgatacgca tcagcggcgc
    40981 aagaagtggt tgctcgccac ggcgataact gtcgataagc gcagccagct gctggcgttc
    41041 tgtggaatca atatgcgggc gaaaataacc ctgaacgtgc atcaaaacgt tggtgtgatc
    41101 gcgcgggttg gcgggatgag aaagcaaaac aattacgcgt tggcggtact ggttgtaaaa
    41161 atcatccaga ttttgccact gatgaatatc agccacaaac gggccaagtt tgcggtactc
    41221 gggctgagaa tgggccagaa agacgagttt atagcggctg tgataatcga gaagcgcccc
    41281 gcgcgttaag ccatgttctt tcagtcgatg taactcatga agggcgaaga tacggattag
    41341 ggtgctgtca tcaaatcgtt gtagattcat tactgctcct gaacggtata caggagtaag
    41401 tgtaggcgtt ggtaaatgat gtttgtgaag ttaatccgct agcagggcaa gattacgaaa
    41461 gcgcgctccc cgcaagcaac taatcaaggt agttaatcat ggctgaacac gatattacgc
    41521 cagaagtcac tatttccaaa acacagcgcc gctataaagt cggctatgtc agagcccgcc
    41581 accaggaccg caaaaccggt atagcccggt attattcaca acatcccagt ctgcacctta
    41641 agggcgactg gctgaaagag gccggatttg ataccggacg gggtgtcacg gtgaagattt
    41701 cggagggggg cctgaccatc atcgctgaca gtgacgaggt gcaggaactg cgggatggga
    41761 tgtttagtgt gctgaacgaa agttaaatat taaaaaagct gggaagattt aaaaccttcc
    41821 tagcttttta ttctgtctta ttataatgga tactcatcat cttccgatga atttataaat
    41881 tgctccaagt ttacatgaag agtacttaac ctagttttta actcattaag tgaagcgata
    41941 atttcatatt ttccaacata atcatatgat ggatcgtatt cattatctat gtcagaagct
    42001 aaaaatgatg atattcgtag tttaaattta gcttcatctt tattacattc attggcataa
    42061 ggtgaatgcc ctatcatagc atattcatct aatctaaaag gataagatgg ttttaattca
    42121 tcgatcaatg aaatttcaat ccatgttcta tatccaggca ttaatggact aatttctttg
    42181 agcaaaacct ttccacttaa taatgtattg agattgttca ttattatgtc tcccacggga
    42241 aaaatatatg tctaacactt ccaccatgtt gatgaacttg taaatgtggt aaatcaccat
    42301 caggatttcc tggcccatga cctgacactc tatatgaagg ttttgttaca gaaggatctt
    42361 ttatatcatc ccaatgatat gaacctgctt ttgttttatt ttcagtaaga tattccatta
    42421 agttcttagt gctggggcct gactcacctg tcatattttt ataattatga cctaaatatc
    42481 tatccataaa caattgttca gcgtcacttc tagacctaac agtaactttg gtttcactac
    42541 tatctaataa acgattcgct ttttggttac taacagcaca tggagttaat ccaagtggat
    42601 cagcatattt tagaggatta ggtgcataac tgtagagatt taaaccgcct ctcaacccta
    42661 tcgggtcagt taccatgaac cgcccgacat ccggatcata gtacctgtgc agattgtagt
    42721 gcagccccgt ttccctgtcg aggtactgtc cctggtagcg caggttctgc tccaccccgc
    42781 cgtggtcctt ctcctgtatc gtgttacccc agacctgata ccccgtctcc cacacgattt
    42841 tcccgtcgct gtccgtcatc tcttccggcg cgccgttcac atccgtgtga aaatacagta
    42901 tccggttcgg gccctctttg cccgccttat ccacccgcgc cagcggttca taactcccct
    42961 ggtccctgta gatgtacagg ctgcctgttc cctccggcct gctctcctgc gccagccgca
    43021 ttccgtccca taaaaatttt gtccggttgt acggcttacc gtcagggctg atgtgctgtt
    43081 tttccgtccg cctccccagt gcatcgtaca cataccggta tgtcccccgc gctgtcgtca
    43141 cttccaccat ccggtgttcc gcatcataac ggtaactctg cgttccgctc cggccctcct
    43201 tttccaccgc acggccaaat tcgtcataac ggtagtgcac accacggtaa gacatcagcc
    43261 ggttgaacgg caccacgctg ttttctgccg tggccctttc ccccgcgcgc ctccccagca
    43321 ggtttcccgc cgcgtccacc tccgtttccg gctggtgcca caccgggtgc cactgcgcct
    43381 gctccacccg ccccagcggg tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat
    43441 agcgccattt gccgccctga gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc
    43501 agcggaatgt cgtcacctgt ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat
    43561 cccactggtg ataacgtgtg cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca
    43621 ggtcccacgc cagccggtac tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg
    43681 ccgcgtcatc cggaccttca taccagcccc actggtaata atacccttcg ccgcccggct
    43741 ggcggtgcag cgtcatgcag tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg
    43801 tgctgtcgta cacgccgttc aggtggccgg cctcgtcata gctgtaacga cgcagcagta
    43861 aaggtgcagt ctcagggaaa tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca
    43921 gttcgtaata cagacggatg cacggacgct gctgttcgcc gctgatttcc acaatgcggc
    43981 cccggtcgtc gtagtacaga ttcagacagt tgctgttacg gtcgcccagc attttcagcc
    44041 gctggcgctg cgggtggtgc gggtcagcct caaacagaaa acactgcccg tcatcgctgc
    44101 tgataagcca gtgcccctgc tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa
    44161 acccgccccc cggcagtacg gcttccatat ccaagcgtcg ccccatgccc gacacaaagg
    44221 tcatgcagtt ttcgtctgcg ctacccggtg tgcacacgag gctgacttca tacattacac
    44281 tccagccctg accaaacatt ccttccgtac gttcgtcacg actgctgtac acgcgctgcc
    44341 agtccagcgg aaagtggccc ggcagactga aatcgaagtc atcgtcatcg gccaggtatt
    44401 tggccccggt ggcggcattg accggatgcg gaagggcact ggtggccttc tgcgtcagca
    44461 tgctcatccc gacactggca gcaaaacagg cgattttaga aagcattttg cccgggcgaa
    44521 gaaacatcag cgccacggtt gccaccaggg ttatctgcga cctgccgctt ctgatatccc
    44581 tgaccaccag taacggaccg ccaatgcgca catccggtga cacaaacgcc ccgttccccg
    44641 gctcattcac caccctggcc tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc
    44701 tgttgatacg cacatcccgc gcgccttccg cgatatattc cgctgcgggc ggctcgtcat
    44761 cgcctttgaa cagattactg atgctgccaa taaccgcatt cactccctga aaggcggttt
    44821 ccagcatacc gcccggattc agcaggttaa gggccgttcc caggggggca tccagaaagg
    44881 cggctttttt ttgtgcgaga aactgcgtca gtgaggaagg atgtttatgg cagtcgattt
    44941 tatcgtcagg ggtcggggtg gatcccgggg ccgcactggc cacagtgggc tgccatagtt
    45001 ccgacacaaa atgtgtcacg ccatccacca tttcagaacg cccttcttcc gtactgagct
    45061 ggtcgtgcag agcccgggac atcagcacag gccctgtcac cgggttaacc gccagggcta
    45121 tggcccccgc catttccaga tattccccgg ccacatcccg gaacgttctg gcatcctcct
    45181 gacgctgcgc ttcttcctgt tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc
    45241 gcgatgccgc agtcgccgct gcccgcgcgg caggtttgct gttgatccat gtgtcaccag
    45301 agccggtgag gatgtaacct tcaatctgag gcgggaaaag catatttgcg agggctgaac
    45361 aggcttcgct gaccttttct gtcacgccgg tgtacaccat cgccacaccc gccacaaaac
    45421 cggtcagcac gacggccgtg caccccatcg tgatccccac tgacgtcgcc agtaacgagg
    45481 ttgtgccgac aatgagggca tcaataacga catttgcggc cacctccagc accgccccca
    45541 gcacatctgc cataaacgac gtatgcagca gggcatcacc ctgtcttgcc gcccagaatg
    45601 cctcttccat gtcccgttcc ctgtatgatt aattcgtcct gacttcctgc cgcatcctga
    45661 agcgcagatt ctttttaatt tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg
    45721 ttctggcgta ggtgaaggcc atcatgaccc gctcacccgg caatacaaat gccacctgac
    45781 actggtacac ctgctcgtta ccccggagaa actggctctg tatttccaaa ccgcctcgtt
    45841 gagataatat tccgtcataa tgtaaatagt ccgttttaat aaaggaatta tttattgaca
    45901 ggttctgttt ttttaatcat gaatgtaata cccatcaccg ctttacagca cactttatct
    45961 ttccagtcat
//
LOCUS       DAJTYL010000018        45308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYL010000018 DAJTYL010000000
VERSION     DAJTYL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.018"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            85..2238
                     /locus_tag="OO572_000993"
     CDS             85..2238
                     /locus_tag="OO572_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6660079.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2291..4021
                     /locus_tag="OO572_000994"
     CDS             2291..4021
                     /locus_tag="OO572_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660080.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4081..4572)
                     /gene="yajQ"
                     /locus_tag="OO572_000995"
     CDS             complement(4081..4572)
                     /gene="yajQ"
                     /locus_tag="OO572_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6660081.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4740..5651
                     /gene="panE"
                     /locus_tag="OO572_000996"
     CDS             4740..5651
                     /gene="panE"
                     /locus_tag="OO572_000996"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6660082.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5614..6204
                     /gene="yajL"
                     /locus_tag="OO572_000997"
     CDS             5614..6204
                     /gene="yajL"
                     /locus_tag="OO572_000997"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6660083.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6258..7706)
                     /gene="thiI"
                     /locus_tag="OO572_000998"
     CDS             complement(6258..7706)
                     /gene="thiI"
                     /locus_tag="OO572_000998"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6660084.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7912..8154
                     /gene="xseB"
                     /locus_tag="OO572_000999"
     CDS             7912..8154
                     /gene="xseB"
                     /locus_tag="OO572_000999"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6660085.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8154..9053
                     /gene="ispA"
                     /locus_tag="OO572_001000"
     CDS             8154..9053
                     /gene="ispA"
                     /locus_tag="OO572_001000"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6660086.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9078..10940
                     /gene="dxs"
                     /locus_tag="OO572_001001"
     CDS             9078..10940
                     /gene="dxs"
                     /locus_tag="OO572_001001"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6660087.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10995..11912
                     /gene="yajO"
                     /locus_tag="OO572_001002"
     CDS             10995..11912
                     /gene="yajO"
                     /locus_tag="OO572_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6660088.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12023..12541)
                     /gene="pgpA"
                     /locus_tag="OO572_001003"
     CDS             complement(12023..12541)
                     /gene="pgpA"
                     /locus_tag="OO572_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6660089.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12519..13496)
                     /gene="thiL"
                     /locus_tag="OO572_001004"
     CDS             complement(12519..13496)
                     /gene="thiL"
                     /locus_tag="OO572_001004"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6660090.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13574..13993)
                     /gene="nusB"
                     /locus_tag="OO572_001005"
     CDS             complement(13574..13993)
                     /gene="nusB"
                     /locus_tag="OO572_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6660091.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14013..14483)
                     /gene="ribE"
                     /locus_tag="OO572_001006"
     CDS             complement(14013..14483)
                     /gene="ribE"
                     /locus_tag="OO572_001006"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6660092.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTVLEMINVLKAIKA"
     gene            complement(14572..15675)
                     /gene="ribD"
                     /locus_tag="OO572_001007"
     CDS             complement(14572..15675)
                     /gene="ribD"
                     /locus_tag="OO572_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6660093.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15679..16128)
                     /gene="nrdR"
                     /locus_tag="OO572_001008"
     CDS             complement(15679..16128)
                     /gene="nrdR"
                     /locus_tag="OO572_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6660094.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16279..16818
                     /locus_tag="OO572_001009"
     CDS             16279..16818
                     /locus_tag="OO572_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6660095.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17116..18000
                     /gene="tsx"
                     /locus_tag="OO572_001010"
     CDS             17116..18000
                     /gene="tsx"
                     /locus_tag="OO572_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6660096.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18177..18524)
                     /locus_tag="OO572_001011"
     CDS             complement(18177..18524)
                     /locus_tag="OO572_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6660097.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18653..19624)
                     /gene="secF"
                     /locus_tag="OO572_001012"
     CDS             complement(18653..19624)
                     /gene="secF"
                     /locus_tag="OO572_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6660098.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19635..21482)
                     /gene="secD"
                     /locus_tag="OO572_001013"
     CDS             complement(19635..21482)
                     /gene="secD"
                     /locus_tag="OO572_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6660099.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21510..21842)
                     /gene="yajC"
                     /locus_tag="OO572_001014"
     CDS             complement(21510..21842)
                     /gene="yajC"
                     /locus_tag="OO572_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6660100.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21865..22992)
                     /gene="tgt"
                     /locus_tag="OO572_001015"
     CDS             complement(21865..22992)
                     /gene="tgt"
                     /locus_tag="OO572_001015"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6660101.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23047..24117)
                     /gene="queA"
                     /locus_tag="OO572_001016"
     CDS             complement(23047..24117)
                     /gene="queA"
                     /locus_tag="OO572_001016"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6660102.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24210..24783
                     /gene="acpH"
                     /locus_tag="OO572_001017"
                     /pseudo
     CDS             24210..24783
                     /gene="acpH"
                     /locus_tag="OO572_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24788..26605)
                     /gene="malZ"
                     /locus_tag="OO572_001018"
     CDS             complement(24788..26605)
                     /gene="malZ"
                     /locus_tag="OO572_001018"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6660103.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26761..28134)
                     /gene="proY"
                     /locus_tag="OO572_001019"
     CDS             complement(26761..28134)
                     /gene="proY"
                     /locus_tag="OO572_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6660104.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28210..29529)
                     /gene="brnQ"
                     /locus_tag="OO572_001020"
     CDS             complement(28210..29529)
                     /gene="brnQ"
                     /locus_tag="OO572_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6660105.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29936..31231)
                     /gene="phoR"
                     /locus_tag="OO572_001021"
     CDS             complement(29936..31231)
                     /gene="phoR"
                     /locus_tag="OO572_001021"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6660106.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31289..31978)
                     /gene="phoB"
                     /locus_tag="OO572_001022"
     CDS             complement(31289..31978)
                     /gene="phoB"
                     /locus_tag="OO572_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6660107.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32168..33370
                     /gene="sbcD"
                     /locus_tag="OO572_001023"
     CDS             32168..33370
                     /gene="sbcD"
                     /locus_tag="OO572_001023"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6660108.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33367..36510
                     /gene="sbcC"
                     /locus_tag="OO572_001024"
     CDS             33367..36510
                     /gene="sbcC"
                     /locus_tag="OO572_001024"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6660109.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36636..37817
                     /gene="araJ"
                     /locus_tag="OO572_001025"
     CDS             36636..37817
                     /gene="araJ"
                     /locus_tag="OO572_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6660110.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37960..38868)
                     /gene="mak"
                     /locus_tag="OO572_001026"
     CDS             complement(37960..38868)
                     /gene="mak"
                     /locus_tag="OO572_001026"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6660111.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38993..39904
                     /gene="rdgC"
                     /locus_tag="OO572_001027"
     CDS             38993..39904
                     /gene="rdgC"
                     /locus_tag="OO572_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6660112.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39982..40437)
                     /locus_tag="OO572_001028"
     CDS             complement(39982..40437)
                     /locus_tag="OO572_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660113.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40434..40769)
                     /locus_tag="OO572_001029"
                     /pseudo
     CDS             complement(40434..40769)
                     /locus_tag="OO572_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40924..41208)
                     /gene="ppnP"
                     /locus_tag="OO572_001030"
     CDS             complement(40924..41208)
                     /gene="ppnP"
                     /locus_tag="OO572_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6660114.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41280..41957)
                     /locus_tag="OO572_001031"
     CDS             complement(41280..41957)
                     /locus_tag="OO572_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6660115.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42215..42406)
                     /gene="yaiA"
                     /locus_tag="OO572_001032"
     CDS             complement(42215..42406)
                     /gene="yaiA"
                     /locus_tag="OO572_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6660116.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42456..42980)
                     /gene="aroL"
                     /locus_tag="OO572_001033"
     CDS             complement(42456..42980)
                     /gene="aroL"
                     /locus_tag="OO572_001033"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6660117.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43163..43621)
                     /locus_tag="OO572_001034"
     CDS             complement(43163..43621)
                     /locus_tag="OO572_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6660118.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43741..44550
                     /gene="proC"
                     /locus_tag="OO572_001035"
     CDS             43741..44550
                     /gene="proC"
                     /locus_tag="OO572_001035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6660119.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44567..>45308)
                     /gene="adrA"
                     /locus_tag="OO572_001036"
                     /gene_synonym="dgcC"
     CDS             complement(44567..>45308)
                     /gene="adrA"
                     /locus_tag="OO572_001036"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6660120.1"
                     /translation="MLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNS
                     APLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETM
                     LRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVI
                     GRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMS
                     HYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11785 a        11810 c        11079 g        10634 t
ORIGIN      
        1 ccatataact ggacgattac attagaagta aactatactt tttatgaaat atcagggata
       61 aattattcac atggaaaata catcatgtac aaatcattat ttactattat cattctactg
      121 tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat aactttcatt
      181 gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat gaaactcgtt
      241 cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc aatttctgct
      301 cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa aaaattagaa
      361 actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga cggtaatggc
      421 actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc tgaatataca
      481 aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa acgtctggca
      541 atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg gaaagtcacc
      601 ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc atttgaagga
      661 tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa atcctggttt
      721 gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag agatggaaaa
      781 tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt ttctaatggt
      841 cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag taaacgtcat
      901 tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga tgggaaaatc
      961 aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga atattatcct
     1021 gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg aaacgatacc
     1081 agttactacc aatccggaaa aataagctct atacacaata gaaattcccg tggtcagtat
     1141 gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc cgtctataaa
     1201 gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga agaggagaaa
     1261 cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa aaatggtaat
     1321 cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga agagtggtac
     1381 gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga cggcattgcc
     1441 aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa tggcgtagag
     1501 aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga aaccctgtac
     1561 gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg taaactatta
     1621 acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta tgatcaaaac
     1681 ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt agacaatcca
     1741 acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc attagggaaa
     1801 tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa atctgcagag
     1861 caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa tggtatacct
     1921 gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg cagtagtaat
     1981 gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca gaatcttcct
     2041 gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc acatttctat
     2101 ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa agccaggttt
     2161 catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt aaatgaactt
     2221 gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac agaaaaggat
     2281 atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg ttttttattc
     2341 tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg tcgagaaaaa
     2401 actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg ctgaatggtt
     2461 taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca atttactgag
     2521 caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg cctccagagt
     2581 cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg ggttaaataa
     2641 accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa gcaaacaacc
     2701 cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt atttaccgac
     2761 tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata attctgtata
     2821 tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag ttgcggaggg
     2881 ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg cgctgaatat
     2941 gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg atgagtattt
     3001 gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg ttttatctca
     3061 acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc tggaaggaat
     3121 tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc tttcaaatta
     3181 catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg aattaagcct
     3241 cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc tggcgtttct
     3301 gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag acaaagtcag
     3361 cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat atttcacgac
     3421 tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa taagtaatac
     3481 tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg catctttcgc
     3541 tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga gaggactatt
     3601 ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa accgagtgtt
     3661 ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta taaatgccaa
     3721 agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta tgcataatct
     3781 gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac acattacctt
     3841 tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg tgggtaatga
     3901 gcaactttat cagattggca aaatctacca taagttggaa attaaacctg agccagagca
     3961 acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg caactcaata
     4021 aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat atccagaaag
     4081 ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc gcaccatcgc
     4141 catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat cgccctgaat
     4201 ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt tctgagtggc
     4261 gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat
     4321 attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag
     4381 aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct
     4441 ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc
     4501 gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc
     4561 gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt
     4621 taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca
     4681 tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa
     4741 tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta
     4801 aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt agtgtgaatc
     4861 tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc
     4921 tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca
     4981 aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac aacggcatgg
     5041 gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc accacccatg
     5101 ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg catatcggtc
     5161 cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa accgtgttgc
     5221 ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact
     5281 gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta cgtcatcatc
     5341 cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc
     5401 atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcagaaa
     5461 atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca
     5521 atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc
     5581 tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc
     5641 gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt tcgcggcggt
     5701 atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac ctgctcgcgc
     5761 ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga atatgacgtg
     5821 atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac tctgctggtt
     5881 gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca
     5941 gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg cttcccgacg
     6001 ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg
     6061 gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct gaaaattatc
     6121 gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca
     6181 gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc
     6241 gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc ttgctcgcgc
     6301 agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag ccaggttttg
     6361 ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca
     6421 atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat atcgagaatc
     6481 acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg ctcggtctgc
     6541 tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg
     6601 aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt
     6661 ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg
     6721 cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgtg
     6781 tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc
     6841 gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga tgcggcacgc
     6901 accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc
     6961 ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg
     7021 ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta
     7081 aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag
     7141 tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccacca
     7201 ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc cagatgaaca
     7261 gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg
     7321 ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt
     7381 acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcaaaaata
     7441 tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata
     7501 cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg aacttcgatg
     7561 ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg
     7621 ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc
     7681 gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac
     7741 ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac
     7801 cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc
     7861 tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag
     7921 aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc
     7981 cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg
     8041 cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg
     8101 ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact
     8161 ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg
     8221 ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag
     8281 gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt ggcgttagca
     8341 caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac tcattaattc
     8401 atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg
     8461 tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct
     8521 cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt tcgatgattt
     8581 ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag
     8641 acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg
     8701 gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc
     8761 gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg
     8821 atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag ggtgccgacc
     8881 agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag
     8941 cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa cagtcactcg
     9001 atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa
     9061 gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg
     9121 actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac
     9181 tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg
     9241 gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt gaccaattga
     9301 tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa
     9361 tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat
     9421 atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg
     9481 ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc gacggcgcga
     9541 ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt cctgatatgc
     9601 tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg cttaacaacc
     9661 atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag
     9721 ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa catattaaag
     9781 gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc ggcccggtgg
     9841 acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc
     9901 cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc
     9961 cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta
    10021 gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga
    10081 aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt
    10141 tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa cacgcagtga
    10201 cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc tactccacct
    10261 tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag ctaccggtcc
    10321 tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac cagggcgctt
    10381 ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg agcgatgaaa
    10441 acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg tccgcggtgc
    10501 gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctaccaattg
    10561 gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt ggtacgctga
    10621 tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat atgcgttttg
    10681 tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca
    10741 ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa gtgctgatgg
    10801 cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag
    10861 gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa
    10921 tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg
    10981 gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac
    11041 tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc
    11101 cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata aatttctttg
    11161 ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc gcactgcggg
    11221 atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc gttggtgatt
    11281 taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgacgtc
    11341 tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg
    11401 aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg
    11461 cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa cagcactgct
    11521 gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa gaagagcgcg
    11581 agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc
    11641 gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct gatgaggtgg
    11701 ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg
    11761 gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttaagta
    11821 aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt gatgagctgc
    11881 tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa acgccgtata
    11941 aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt
    12001 cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa
    12061 agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc
    12121 cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac
    12181 ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg
    12241 tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat
    12301 ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg
    12361 agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc gccagcgatc
    12421 ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc
    12481 acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac aaaatggtca
    12541 tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
    12601 tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc
    12661 agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag
    12721 agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc
    12781 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
    12841 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
    12901 tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca
    12961 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta
    13021 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
    13081 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
    13141 atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc
    13201 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
    13261 atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg
    13321 atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt
    13381 ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga
    13441 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
    13501 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt ccgtgaatct
    13561 accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
    13621 cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt
    13681 taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc gcaatgcgca
    13741 gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag ggcttcatca
    13801 gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga
    13861 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
    13921 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
    13981 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
    14041 atcatttcaa gcacggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
    14101 cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg
    14161 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg
    14221 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
    14281 gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg
    14341 ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg
    14401 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
    14461 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
    14521 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
    14581 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
    14641 ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt cgctgcctaa
    14701 tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta gcaatacgcc
    14761 agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac ccagttgcat
    14821 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
    14881 ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg
    14941 cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg
    15001 gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac
    15061 tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg
    15121 tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc
    15181 gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa
    15241 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag
    15301 cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg
    15361 cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc
    15421 acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc
    15481 gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc
    15541 tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
    15601 cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta
    15661 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
    15721 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
    15781 caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc
    15841 gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac tcaccggacg
    15901 tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac
    15961 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
    16021 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
    16081 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
    16141 ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
    16201 ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg
    16261 acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg
    16321 ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc
    16381 acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac
    16441 aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca
    16501 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca
    16561 gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc
    16621 agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
    16681 gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt
    16741 ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt
    16801 caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatcttccc
    16861 cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc
    16921 gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa
    16981 tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc
    17041 tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca
    17101 aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct
    17161 tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg
    17221 caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac
    17281 gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat
    17341 gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt
    17401 tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac
    17461 cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt
    17521 cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact
    17581 ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc
    17641 gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt
    17701 accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc
    17761 gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct
    17821 atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct
    17881 cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc
    17941 aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga
    18001 ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt
    18061 catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat
    18121 tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac
    18181 ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca
    18241 tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat
    18301 ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact gccatcttcc
    18361 gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttagaataa
    18421 acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga
    18481 tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag ttacctggat
    18541 gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc
    18601 aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt gattaaggca
    18661 gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct
    18721 tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg
    18781 aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca
    18841 gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg
    18901 tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac
    18961 gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg
    19021 atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga
    19081 taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac
    19141 ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa
    19201 ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg
    19261 tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg gtttcgcctt
    19321 cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct
    19381 cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga
    19441 gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc
    19501 ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc
    19561 agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg
    19621 ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt
    19681 acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta
    19741 gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc
    19801 agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt
    19861 gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg
    19921 ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt
    19981 gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca
    20041 ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc
    20101 agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca
    20161 attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt
    20221 gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga
    20281 cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca
    20341 tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg
    20401 atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta
    20461 acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccagtc
    20521 agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag
    20581 tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg
    20641 aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaataccg
    20701 ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca
    20761 agttggttta cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct
    20821 tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc
    20881 accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg
    20941 cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc
    21001 ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg
    21061 gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc
    21121 atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc
    21181 acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca
    21241 gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt
    21301 ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg
    21361 cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc
    21421 agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac
    21481 acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg
    21541 acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca
    21601 atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact
    21661 tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt
    21721 ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac
    21781 atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc
    21841 attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg
    21901 acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa
    21961 acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa
    22021 tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta
    22081 gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat tgcggatttt
    22141 caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca
    22201 gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc
    22261 catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca gaatacggtg
    22321 catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat caaagccgat
    22381 atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc cctgaattat
    22441 accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac gcttcgccca
    22501 acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat acggcgtaca
    22561 ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca tcgacttttc
    22621 cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc cctgttcggt
    22681 gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg taagaatcgg
    22741 tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg
    22801 gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc
    22861 ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc
    22921 cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag
    22981 ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta aaccagcgcc
    23041 gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga taaacatcgc
    23101 atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg cattcatggt
    23161 gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg gcaagtggaa
    23221 gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa agatttgggt
    23281 atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga
    23341 acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac
    23401 cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt gatcttcaat
    23461 ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat
    23521 ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca gaccagcggt
    23581 cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt cgcggtcagc
    23641 gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc tgttgaggat
    23701 atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt
    23761 cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc
    23821 tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct caaccagcac
    23881 ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca cgcgggtatt
    23941 attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga aagtaccgtg
    24001 cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc gttcaggcat
    24061 gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac
    24121 actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc
    24181 tttacccccc tttggataga atataatcga tgaatttttt agctcacctg catttagccc
    24241 atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc ggaaatcctg
    24301 aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt attgactgac
    24361 aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg ccgcgttgcg
    24421 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    24481 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    24541 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    24601 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    24661 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    24721 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    24781 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    24841 tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac ggtgacgcgg
    24901 gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt tgctgattca
    24961 gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag ccgccacgac
    25021 gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg aataacgccg
    25081 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttaccat
    25141 ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    25201 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    25261 cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    25321 aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca taagagatgt
    25381 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcagcat
    25441 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    25501 cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc atattattgc
    25561 gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg tccatattcc
    25621 acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    25681 gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag tcgagcgccg
    25741 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg tgacaagctc
    25801 cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    25861 cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa cgcagcaacg
    25921 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    25981 cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc tttttcagat
    26041 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    26101 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    26161 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    26221 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca tcgacggcaa
    26281 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    26341 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    26401 tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta tgcattggta
    26461 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    26521 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    26581 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    26641 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    26701 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagta
    26761 ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc caatcaacag
    26821 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    26881 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    26941 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga aggcgatttg
    27001 cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg aagcgatcac
    27061 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    27121 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    27181 tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata cgtcactgtt
    27241 aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    27301 gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct gattccacgg
    27361 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    27421 ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    27481 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta ccatgccaag
    27541 ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg tcggttgccc
    27601 gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga tgatgatggt
    27661 ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca cgctcatcag
    27721 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    27781 gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    27841 ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt tttcctgcgc
    27901 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    27961 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    28021 tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac ccagtgccat
    28081 aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt ccatcaattc
    28141 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    28201 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    28261 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    28321 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    28381 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    28441 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    28501 caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca ccatcgagaa
    28561 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    28621 tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga ttaacgccgc
    28681 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    28741 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga acagcgccag
    28801 gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    28861 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    28921 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaatgcagc
    28981 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcgacaac
    29041 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    29101 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    29161 aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa aggaaacggt
    29221 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    29281 tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    29341 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    29401 agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa tgttacctgc
    29461 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    29521 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    29581 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    29641 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    29701 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    29761 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    29821 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    29881 aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag gcggattaat
    29941 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    30001 ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc acgatcgcta
    30061 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    30121 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    30181 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc ggcgtatgat
    30241 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    30301 ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    30361 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    30421 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    30481 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    30541 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    30601 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    30661 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    30721 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    30781 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    30841 gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg gtcagcacca
    30901 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    30961 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    31021 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    31081 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    31141 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    31201 ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag
    31261 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    31321 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    31381 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    31441 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    31501 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    31561 tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa atacggcgca
    31621 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg atatagtcat
    31681 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    31741 ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac tggataccgg
    31801 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    31861 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    31921 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    31981 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    32041 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    32101 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt ttcgccaggg
    32161 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    32221 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    32281 tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc cgcccagtta
    32341 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    32401 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    32461 gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    32521 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc gtgacattat
    32581 ttccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    32641 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    32701 catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg ccgtgcgtga
    32761 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    32821 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    32881 cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    32941 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taactcaacc
    33001 catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    33061 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    33121 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    33181 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    33241 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc tggatgaatc
    33301 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg ccggagaaca
    33361 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    33421 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat taccggccca
    33481 accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    33541 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgctgaa
    33601 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt ctggagccag
    33661 aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga gctggcgcgc
    33721 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    33781 ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt
    33841 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    33901 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcact
    33961 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    34021 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    34081 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    34141 gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    34201 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    34261 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    34321 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    34381 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    34441 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    34501 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    34561 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat
    34621 gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    34681 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    34741 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    34801 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc ccagcgtgca
    34861 cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    34921 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    34981 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    35041 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    35101 caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca ggacgatatt
    35161 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    35221 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    35281 attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact gacattgcca
    35341 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    35401 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac accgattctg
    35461 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    35521 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    35581 caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga caccgcgcta
    35641 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    35701 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    35761 ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    35821 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    35881 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    35941 cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca ggttgaggac
    36001 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    36061 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    36121 gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    36181 tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    36241 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    36301 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    36361 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgtagaagcg
    36421 atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc
    36481 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    36541 tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    36601 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    36661 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg ctcacggagc
    36721 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    36781 tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    36841 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    36901 cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat ggcgcatttt
    36961 ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    37021 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    37081 atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt aatattgcgg
    37141 tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa ggaaatctgc
    37201 gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc gccacgatgt
    37261 ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt
    37321 ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgc
    37381 tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag
    37441 tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa
    37501 caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc
    37561 tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc gcaggtgggc
    37621 aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc
    37681 tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt
    37741 catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg
    37801 cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg atgcggcgtg
    37861 aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa
    37921 gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca
    37981 cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg gcgtttcaca
    38041 ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata aacggtctac
    38101 attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc
    38161 cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct
    38221 ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccactca aacgacgata
    38281 atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata
    38341 acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg
    38401 tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga atgccacgcc
    38461 cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc
    38521 atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca cttcccgctg
    38581 caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattgacatt
    38641 cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta ccgttccgcg
    38701 ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg
    38761 gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag
    38821 tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg tactctcctt
    38881 atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg
    38941 acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg
    39001 gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat
    39061 ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat
    39121 gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca
    39181 aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga ttaaacaggc
    39241 gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga
    39301 aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt
    39361 tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg actgcgccag
    39421 tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt cgttaccggt
    39481 tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg ttcgctccgg
    39541 tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga
    39601 tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat
    39661 tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt
    39721 gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc gcgatcaaaa
    39781 cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac
    39841 tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg
    39901 ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac
    39961 gaagccacac caggcatata attattcgct acggcgagca ataattttta gcgcagcaat
    40021 attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc tgtcagcagc
    40081 acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta acagctccgc
    40141 ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat ccgtcaggta
    40201 aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg caataaagtc
    40261 tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg gtaaatcggt
    40321 acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca ggttgatcgt
    40381 tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt gtgtcattgt
    40441 ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact
    40501 aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct
    40561 tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc cagcgaccga
    40621 tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc
    40681 gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat
    40741 ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact gatatttata
    40801 tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa aggaaatatt
    40861 attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag
    40921 gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat gaaactcact
    40981 gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg
    41041 cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa
    41101 ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa
    41161 gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg
    41221 tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt cacgcaaaat
    41281 tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa gacaggaaca
    41341 tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg
    41401 atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt ttgttctgaa
    41461 tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc
    41521 gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg atcttcaaca
    41581 atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt attacgcgca
    41641 gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc ctgattatcg
    41701 agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac atgggccagc
    41761 tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc
    41821 atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt atcttcgtca
    41881 atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat ggtcaggatc
    41941 gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca
    42001 agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca
    42061 gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg ttattgttta
    42121 atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc ccttgtagtg
    42181 gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg tcctcatagc
    42241 gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac gagtctggtt
    42301 taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa
    42361 tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt
    42421 tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt ctgtgccagg
    42481 gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc
    42541 gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc
    42601 ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc
    42661 aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga
    42721 ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc cgcagttacc
    42781 gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc
    42841 tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg
    42901 ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc
    42961 agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg
    43021 gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt aaagaaaaaa
    43081 ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata ttcaagaatg
    43141 aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc caccacttct
    43201 ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct
    43261 ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg
    43321 tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc tcgatggctt
    43381 cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa
    43441 tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg
    43501 gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg
    43561 cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca
    43621 taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt
    43681 tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca
    43741 atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt
    43801 ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat
    43861 aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg
    43921 gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat taaagtgctt
    43981 agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc
    44041 acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg
    44101 aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc
    44161 ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt
    44221 gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt
    44281 atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg
    44341 tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa
    44401 catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta
    44461 cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg
    44521 gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt
    44581 cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc
    44641 actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag
    44701 ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc cgtaacatgg
    44761 cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca tcgccgccaa
    44821 accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa
    44881 tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa tggtcgatat
    44941 cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta
    45001 acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga gtactcatga
    45061 cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa
    45121 acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc ggagcactat
    45181 tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc accatcaaca
    45241 ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga cacagaatca
    45301 tcaacata
//
LOCUS       DAJTYL010000019        42522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYL010000019 DAJTYL010000000
VERSION     DAJTYL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.019"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO572_001037"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO572_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6660121.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="OO572_001038"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="OO572_001038"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6660122.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3051..4091
                     /locus_tag="OO572_001039"
     CDS             3051..4091
                     /locus_tag="OO572_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6660123.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="OO572_001040"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="OO572_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6660124.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="OO572_001041"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="OO572_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6660125.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="OO572_001042"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="OO572_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6660126.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="OO572_001043"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="OO572_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6660127.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="OO572_001044"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="OO572_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6660128.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="OO572_001045"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="OO572_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6660129.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="OO572_001046"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="OO572_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6660130.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="OO572_001047"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="OO572_001047"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6660131.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11837..13507
                     /gene="hscC"
                     /locus_tag="OO572_001048"
                     /pseudo
     CDS             11837..13507
                     /gene="hscC"
                     /locus_tag="OO572_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="OO572_001049"
     CDS             complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="OO572_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6660132.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15015..15722)
                     /locus_tag="OO572_001050"
     CDS             complement(15015..15722)
                     /locus_tag="OO572_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6660133.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15824..16378
                     /locus_tag="OO572_001051"
     CDS             15824..16378
                     /locus_tag="OO572_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6660134.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16388..17815)
                     /locus_tag="OO572_001052"
     CDS             complement(16388..17815)
                     /locus_tag="OO572_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6660135.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17812..18519)
                     /locus_tag="OO572_001053"
     CDS             complement(17812..18519)
                     /locus_tag="OO572_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6660136.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18683..19660
                     /locus_tag="OO572_001054"
     CDS             18683..19660
                     /locus_tag="OO572_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6660137.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19730..20212)
                     /locus_tag="OO572_001055"
     CDS             complement(19730..20212)
                     /locus_tag="OO572_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6660138.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20447..23029
                     /gene="leuS"
                     /locus_tag="OO572_001056"
     CDS             20447..23029
                     /gene="leuS"
                     /locus_tag="OO572_001056"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6660139.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23044..23625
                     /gene="lptE"
                     /locus_tag="OO572_001057"
     CDS             23044..23625
                     /gene="lptE"
                     /locus_tag="OO572_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6660140.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23625..24656
                     /gene="holA"
                     /locus_tag="OO572_001058"
     CDS             23625..24656
                     /gene="holA"
                     /locus_tag="OO572_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6660141.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24658..25299
                     /gene="nadD"
                     /locus_tag="OO572_001059"
     CDS             24658..25299
                     /gene="nadD"
                     /locus_tag="OO572_001059"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6660142.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25323..25934
                     /locus_tag="OO572_001060"
     CDS             25323..25934
                     /locus_tag="OO572_001060"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6660143.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26194..26511
                     /gene="rsfS"
                     /locus_tag="OO572_001061"
                     /gene_synonym="rsfA"
     CDS             26194..26511
                     /gene="rsfS"
                     /locus_tag="OO572_001061"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6660144.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26515..26982
                     /gene="rlmH"
                     /locus_tag="OO572_001062"
     CDS             26515..26982
                     /gene="rlmH"
                     /locus_tag="OO572_001062"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6660145.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27013..28914
                     /gene="mrdA"
                     /locus_tag="OO572_001063"
     CDS             27013..28914
                     /gene="mrdA"
                     /locus_tag="OO572_001063"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6660146.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28917..30029
                     /gene="mrdB"
                     /locus_tag="OO572_001064"
                     /gene_synonym="rodA"
     CDS             28917..30029
                     /gene="mrdB"
                     /locus_tag="OO572_001064"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6660147.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30040..31128
                     /gene="rlpA"
                     /locus_tag="OO572_001065"
     CDS             30040..31128
                     /gene="rlpA"
                     /locus_tag="OO572_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6660148.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31267..32478
                     /gene="dacA"
                     /locus_tag="OO572_001066"
     CDS             31267..32478
                     /gene="dacA"
                     /locus_tag="OO572_001066"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6660149.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32589..32852
                     /locus_tag="OO572_001067"
     CDS             32589..32852
                     /locus_tag="OO572_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6660150.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32953..33594
                     /gene="lipB"
                     /locus_tag="OO572_001068"
     CDS             32953..33594
                     /gene="lipB"
                     /locus_tag="OO572_001068"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6660151.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33853..34806
                     /locus_tag="OO572_001069"
     CDS             33853..34806
                     /locus_tag="OO572_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6660152.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35015..35980
                     /gene="lipA"
                     /locus_tag="OO572_001070"
     CDS             35015..35980
                     /gene="lipA"
                     /locus_tag="OO572_001070"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6660153.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="OO572_001071"
     CDS             complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="OO572_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6660154.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36413..37201)
                     /locus_tag="OO572_001072"
     CDS             complement(36413..37201)
                     /locus_tag="OO572_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6660155.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37294..37677
                     /gene="crcB"
                     /locus_tag="OO572_001073"
     CDS             37294..37677
                     /gene="crcB"
                     /locus_tag="OO572_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6660156.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="OO572_001074"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="OO572_001074"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6660157.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38115..38675)
                     /gene="pagP"
                     /locus_tag="OO572_001075"
     CDS             complement(38115..38675)
                     /gene="pagP"
                     /locus_tag="OO572_001075"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6660158.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39264..40649
                     /gene="dcuC"
                     /locus_tag="OO572_001076"
     CDS             39264..40649
                     /gene="dcuC"
                     /locus_tag="OO572_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6660159.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40690..41031)
                     /locus_tag="OO572_001077"
                     /pseudo
     CDS             complement(40690..41031)
                     /locus_tag="OO572_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41369..41878)
                     /locus_tag="OO572_001078"
     CDS             complement(41369..41878)
                     /locus_tag="OO572_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6660160.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42041..42286
                     /locus_tag="OO572_001079"
     CDS             42041..42286
                     /locus_tag="OO572_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6660161.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42261..>42522
                     /locus_tag="OO572_001080"
     CDS             42261..>42522
                     /locus_tag="OO572_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6660162.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYD"
BASE COUNT        10698 a        10658 c        10560 g        10606 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagc actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    10381 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    10441 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    10741 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    10861 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    11161 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    11341 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    11401 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    11701 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    11761 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    11821 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    11881 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    11941 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    12001 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    12061 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    12121 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    12181 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    12241 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    12301 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    12361 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    12421 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    12481 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    12541 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    12601 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    12661 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    12721 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    12781 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    12841 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    12901 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    12961 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    13021 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    13081 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    13141 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    13201 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    13261 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    13321 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    13381 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    13441 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    13501 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    13561 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    13621 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    13681 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    13741 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    13801 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    13861 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    13921 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    13981 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    14041 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    14101 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    14161 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    14221 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    14281 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    14341 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    14401 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    14461 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    14521 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    14581 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    14641 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    14701 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    14761 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    14821 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    14881 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    14941 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    15001 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    15061 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    15121 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    15181 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    15241 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    15301 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    15361 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    15421 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    15481 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    15541 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    15601 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    15661 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    15721 attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg atgagtataa
    15781 acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata aaaaattaat
    15841 gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac ccaagaaaac
    15901 gatgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat caccaggcag
    15961 tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact caacatcgca
    16021 atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
    16081 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
    16141 ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag caattctctg
    16201 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg gtttgatcta
    16261 cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
    16321 cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg cacattaacg
    16381 tcgactgtca cccgaagaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
    16441 agaaaataat tttgatgacg cccagacagc ccagcccgcc atcatcaacg ggcattggct
    16501 gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc tgaattaata
    16561 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg cggctcacaa
    16621 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
    16681 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
    16741 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
    16801 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agcggttttc
    16861 cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac cactaatggg taataataga
    16921 cccgatgctg acggcaccat tgcaacatta aagcatccgc ctgggtggag ggatactcgc
    16981 gccagtagta acaaagttct gccagcaggc tttcgccttc tccacaatag acgcgttgtg
    17041 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
    17101 aatccaggag ttcggggatt cccaggcggc ttgcgctata ccagcggagt aaatttctgt
    17161 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
    17221 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
    17281 gcacggcaac tggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
    17341 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
    17401 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
    17461 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
    17521 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
    17581 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
    17641 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
    17701 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
    17761 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
    17821 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
    17881 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
    17941 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
    18001 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
    18061 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
    18121 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
    18181 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
    18241 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
    18301 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttatgcgcag
    18361 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
    18421 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
    18481 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
    18541 atttaatgaa tgtaaactac atttcgaggc tcagttattt atacttttct gacgcaacat
    18601 aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa tatattttat
    18661 ctacaaccat aaggaaatga taatgatttt tacgtcaagt tgctgcgata atttatcaat
    18721 agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt
    18781 ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct tttactggct
    18841 gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc aaaagtatac
    18901 cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga aaaaagctgc
    18961 cctacaaggc catactttcg cttccaacgc ccttggctgg atactggatc gtggagaaga
    19021 ccccaactat aaagaagcgg ttacctggta tcagatagcc gcggagagcg gaatgtctta
    19081 tgcgcaaaat aatcttggat ggatgtacag aaatggcaac ggagtcgcac aagactatac
    19141 gctggcattt ttctggtaca aacaagctgc attacaaggc catagttacg cacaagacaa
    19201 tctggccgat ctttataaag acggagaagg cgttgctcaa aacaagacac tcgccgcatt
    19261 ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga
    19321 ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact ggtatctgaa
    19381 ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt ataaacacgg
    19441 acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg
    19501 caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag aaggaagacc
    19561 tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga
    19621 tgtcagtcag gaaattcgtg agactgaagc tttactgtag aaataaaaac aagggccggg
    19681 gaatttcatt ctgcccatat tctctttatt gctgtgcgaa atcagactat tacggctcaa
    19741 acgggcgtct ctggaactgg tcataaccac atttcgggca tagcgtcagc acttccggtg
    19801 tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc ccgaccactt
    19861 ctccgctgtg ataaacccca tgatgattga ggtcctggaa aatttcgcgc cattcaagct
    19921 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    19981 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    20041 cacgtctgac agctcgcgtc agctcatcga tctcggttcg cgttaactcc cctgttttta
    20101 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    20161 gttcgttcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    20221 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    20281 gcccacaaac tctgtaaatt aatgtacgaa taatccgggc atcatttcaa cgtcgagtgc
    20341 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    20401 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    20461 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    20521 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    20581 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta
    20641 ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc
    20701 tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt acgacaacat
    20761 cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    20821 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    20881 aaaaggcctg gtatataaaa agacttctgc ggtcaactgg tgcccgaacg accagaccgt
    20941 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg
    21001 taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc tgctcaacga
    21061 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    21121 tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    21181 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag ctgcgggtca
    21241 tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg
    21301 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    21361 tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    21421 cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    21481 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg
    21541 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    21601 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    21661 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgcca
    21721 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccaac
    21781 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    21841 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    21901 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    21961 cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga
    22021 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    22081 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg
    22141 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    22201 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    22261 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    22321 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    22381 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg
    22441 ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcacacag caaaaggtga
    22501 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    22561 gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    22621 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    22681 ggatcgcgct ctgatgcagg aagcactgca ggccgttgtc cgtatgctca acccgttcac
    22741 cccgcacatc tgcttcacgc tgtggcagga actcaaaggc gaaggcgata tcgacaacgc
    22801 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    22861 ggttaacggt aaagtccgtg ccaaaatcac cgtcccggtg gacgcaacgg aagaacaggt
    22921 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg
    22981 taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga
    23041 agcgtgcgat atctggcaac actgttgtta tctctggcgg tgttaatcac cgccgggtgt
    23101 ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    23161 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt
    23221 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    23281 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    23341 atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc
    23401 gccaaagtct tccgttcatt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    23461 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    23521 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    23581 actccggcaa cgcctgcacg cgtctccacc atgctgggta actgatgatt cggttgtacc
    23641 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    23701 acgatcctct gttattgcag gaaagccagg acgctgttcg ccaggtagct gcggcacaag
    23761 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    23821 cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    23881 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    23941 acgacctgct attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct
    24001 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    24061 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagaactg gatgacgcgg
    24121 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    24181 agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt gaacaggcgg
    24241 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    24301 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt
    24361 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    24421 atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    24481 tgggcgaggc gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga
    24541 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    24601 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    24661 aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatctaaaa
    24721 cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat
    24781 gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa
    24841 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    24901 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg
    24961 ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    25021 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    25081 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    25141 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    25201 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    25261 gtattgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    25321 tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca
    25381 gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata
    25441 cactgctaga tgatgtttcc tttgacttgg ttttatgcag tgaactggaa cgggcacagc
    25501 ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca
    25561 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    25621 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    25681 gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc
    25741 actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt
    25801 taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg
    25861 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    25921 tcgagaacgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    25981 cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat
    26041 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    26101 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    26161 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    26221 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc
    26281 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg
    26341 tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    26401 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    26461 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    26521 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    26581 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggcaaa
    26641 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    26701 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    26761 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtaag tctactgatt
    26821 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg
    26881 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    26941 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    27001 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    27061 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    27121 gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa
    27181 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    27241 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    27301 aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga tgacgatatt
    27361 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact
    27421 aacctaaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc
    27481 gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc
    27541 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    27601 gccaactatg cggcaacgca tgatattggt aagctgggca ttgagcgtta ctatgaagac
    27661 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    27721 cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc
    27781 aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgttacc
    27841 gaaccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    27901 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    27961 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    28021 gcggtttcgg cattaagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc
    28081 tggtggcaac taccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    28141 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    28201 tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    28261 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    28321 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggccagggt
    28381 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    28441 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    28501 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa
    28561 gacggtatgt acggtgttgc taaccgccct aatggtacgg cgaataaata ctttgccagc
    28621 gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct gaaagcgaac
    28681 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    28741 tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggcggcgcg
    28801 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    28861 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    28921 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    28981 tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    29041 atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg
    29101 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    29161 gtattatttt gctggtggcg gtagatgctt tcggagccat ctctaaaggc gctcaacgct
    29221 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    29281 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aatactggca
    29341 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    29401 catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc
    29461 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    29521 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    29581 tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    29641 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccatactg
    29701 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg
    29761 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    29821 ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    29881 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    29941 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    30001 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    30061 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    30121 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    30181 ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc
    30241 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    30301 gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac aaagctgacg
    30361 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    30421 cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg
    30481 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    30541 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc
    30601 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    30661 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    30721 gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg
    30781 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagcttcg
    30841 tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    30901 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    30961 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    31021 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    31081 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    31141 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    31201 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    31261 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    31321 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    31381 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    31441 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    31501 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    31561 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    31621 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    31681 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    31741 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    31801 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    31861 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    31921 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    31981 gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc ttctgcgact
    32041 gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa aggccgtgaa
    32101 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    32161 aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga tcgcgcttcg
    32221 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    32281 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    32341 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    32401 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    32461 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    32521 agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    32581 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    32641 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    32701 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    32761 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    32821 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    32881 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    32941 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    33001 gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag tacccttgat
    33061 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    33121 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    33181 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    33241 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    33301 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    33361 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    33421 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    33481 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    33541 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    33601 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    33661 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    33721 gtaactcacc ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc
    33781 gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat
    33841 tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca
    33901 gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt
    33961 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
    34021 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
    34081 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
    34141 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
    34201 cgaacgataa ctattgcgac cactccctcg gtcggagcct tggtccttcc tgtcatctat
    34261 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
    34321 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
    34381 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcaaggga
    34441 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
    34501 ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg
    34561 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
    34621 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg
    34681 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
    34741 aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
    34801 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
    34861 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
    34921 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
    34981 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
    35041 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
    35101 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
    35161 tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga
    35221 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
    35281 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
    35341 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
    35401 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccaacactt
    35461 tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
    35521 gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa cgcccccaga
    35581 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac ggcctggcgc
    35641 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
    35701 gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta ttgaggtaat
    35761 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
    35821 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
    35881 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
    35941 ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata attacttaca
    36001 tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag
    36061 ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg
    36121 tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg
    36181 gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc
    36241 agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca
    36301 tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc
    36361 gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata
    36421 attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc acctgacgca
    36481 cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg
    36541 tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg
    36601 ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat
    36661 gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca gctccctgta
    36721 aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct
    36781 tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt
    36841 cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg
    36901 cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga tgaatcgtca
    36961 gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt
    37021 ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg
    37081 cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac
    37141 aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca gcaaccagca
    37201 ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta
    37261 tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt
    37321 attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt taacccgctg
    37381 catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga
    37441 atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc
    37501 accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt
    37561 ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa ccttctgggg
    37621 tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt acactaaaga
    37681 ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    37741 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    37801 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    37861 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    37921 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    37981 tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    38041 taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa gaagttacta
    38101 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    38161 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    38221 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    38281 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    38341 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    38401 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    38461 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    38521 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    38581 gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    38641 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    38701 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    38761 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    38821 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    38881 ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac gagatacaac
    38941 aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt agattatttt
    39001 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
    39061 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
    39121 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
    39181 atcgccaatt tattgggcat attttttcct taagctttag gaagttttta ttatttactt
    39241 tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    39301 ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    39361 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    39421 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    39481 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca
    39541 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    39601 ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg
    39661 ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt ccggtgatgg
    39721 taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta
    39781 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga
    39841 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    39901 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    39961 tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc
    40021 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    40081 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    40141 agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg
    40201 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    40261 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    40321 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    40381 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    40441 tgactattcc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg
    40501 tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct
    40561 cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag
    40621 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    40681 tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat
    40741 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    40801 gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    40861 cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg
    40921 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    40981 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat
    41041 tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag
    41101 atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa
    41161 agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc
    41221 atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcatcaaatg
    41281 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
    41341 gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca
    41401 acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc
    41461 gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac
    41521 ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa
    41581 gcccatattt agccatgttt agctctatct ctcggacttc attttctcct agtttattga
    41641 ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca
    41701 tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct
    41761 tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat
    41821 ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa
    41881 actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa
    41941 ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca agacgaatta
    42001 tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga
    42061 ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt
    42121 gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc
    42181 taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag
    42241 tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat
    42301 ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca
    42361 agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta
    42421 acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt
    42481 ttgaaatcgc ccgccccacg ctggaagcgc acgattacga tc
//
LOCUS       DAJTYL010000020        42503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYL010000020 DAJTYL010000000
VERSION     DAJTYL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.020"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(78..614)
                     /gene="ssb1"
                     /locus_tag="OO572_001081"
     CDS             complement(78..614)
                     /gene="ssb1"
                     /locus_tag="OO572_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6660163.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            868..3690
                     /gene="uvrA"
                     /locus_tag="OO572_001082"
     CDS             868..3690
                     /gene="uvrA"
                     /locus_tag="OO572_001082"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6660164.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3725..4081)
                     /locus_tag="OO572_001083"
     CDS             complement(3725..4081)
                     /locus_tag="OO572_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6660165.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4085..4501)
                     /locus_tag="OO572_001084"
     CDS             complement(4085..4501)
                     /locus_tag="OO572_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6660166.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4612..5325)
                     /gene="aphA"
                     /locus_tag="OO572_001085"
     CDS             complement(4612..5325)
                     /gene="aphA"
                     /locus_tag="OO572_001085"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6660167.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5727..5931
                     /locus_tag="OO572_001086"
                     /pseudo
     CDS             5727..5931
                     /locus_tag="OO572_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6434..7627)
                     /gene="tyrB"
                     /locus_tag="OO572_001087"
     CDS             complement(6434..7627)
                     /gene="tyrB"
                     /locus_tag="OO572_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6660168.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7880..8959)
                     /gene="alr"
                     /locus_tag="OO572_001088"
     CDS             complement(7880..8959)
                     /gene="alr"
                     /locus_tag="OO572_001088"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6660169.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9012..10427)
                     /gene="dnaB"
                     /locus_tag="OO572_001089"
     CDS             complement(9012..10427)
                     /gene="dnaB"
                     /locus_tag="OO572_001089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6660170.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10510..11493
                     /locus_tag="OO572_001090"
     CDS             10510..11493
                     /locus_tag="OO572_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6660171.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11659..11901)
                     /gene="pspG"
                     /locus_tag="OO572_001091"
     CDS             complement(11659..11901)
                     /gene="pspG"
                     /locus_tag="OO572_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6660172.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12035..13072)
                     /gene="dusA"
                     /locus_tag="OO572_001092"
     CDS             complement(12035..13072)
                     /gene="dusA"
                     /locus_tag="OO572_001092"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6660173.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13435..14420)
                     /locus_tag="OO572_001093"
                     /pseudo
     CDS             complement(13435..14420)
                     /locus_tag="OO572_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14738..15253
                     /gene="zur"
                     /locus_tag="OO572_001094"
     CDS             14738..15253
                     /gene="zur"
                     /locus_tag="OO572_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6660174.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15295..15504)
                     /locus_tag="OO572_001095"
     CDS             complement(15295..15504)
                     /locus_tag="OO572_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6660175.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15620..16945)
                     /gene="dinF"
                     /locus_tag="OO572_001096"
     CDS             complement(15620..16945)
                     /gene="dinF"
                     /locus_tag="OO572_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6660176.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17018..17626)
                     /gene="lexA"
                     /locus_tag="OO572_001097"
     CDS             complement(17018..17626)
                     /gene="lexA"
                     /locus_tag="OO572_001097"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6660177.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17736..18104)
                     /gene="dgkA"
                     /locus_tag="OO572_001098"
     CDS             complement(17736..18104)
                     /gene="dgkA"
                     /locus_tag="OO572_001098"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6660178.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18275..20698
                     /gene="plsB"
                     /locus_tag="OO572_001099"
     CDS             18275..20698
                     /gene="plsB"
                     /locus_tag="OO572_001099"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6660179.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20853..21725)
                     /gene="ubiA"
                     /locus_tag="OO572_001100"
     CDS             complement(20853..21725)
                     /gene="ubiA"
                     /locus_tag="OO572_001100"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6660180.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21738..22235)
                     /gene="ubiC"
                     /locus_tag="OO572_001101"
     CDS             complement(21738..22235)
                     /gene="ubiC"
                     /locus_tag="OO572_001101"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6660181.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22458..24038)
                     /locus_tag="OO572_001102"
                     /pseudo
     CDS             complement(22458..24038)
                     /locus_tag="OO572_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24267..25187)
                     /gene="malM"
                     /locus_tag="OO572_001103"
     CDS             complement(24267..25187)
                     /gene="malM"
                     /locus_tag="OO572_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6660182.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25339..26679)
                     /gene="lamB"
                     /locus_tag="OO572_001104"
     CDS             complement(25339..26679)
                     /gene="lamB"
                     /locus_tag="OO572_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6660183.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDGIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26751..27866)
                     /gene="malK"
                     /locus_tag="OO572_001105"
     CDS             complement(26751..27866)
                     /gene="malK"
                     /locus_tag="OO572_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6660184.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28231..29421
                     /gene="malE"
                     /locus_tag="OO572_001106"
     CDS             28231..29421
                     /gene="malE"
                     /locus_tag="OO572_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6660185.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29575..31119
                     /gene="malF"
                     /locus_tag="OO572_001107"
     CDS             29575..31119
                     /gene="malF"
                     /locus_tag="OO572_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6660186.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31134..32024
                     /gene="malG"
                     /locus_tag="OO572_001108"
     CDS             31134..32024
                     /gene="malG"
                     /locus_tag="OO572_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6660187.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPISI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32395..33870
                     /gene="xylE"
                     /locus_tag="OO572_001109"
     CDS             32395..33870
                     /gene="xylE"
                     /locus_tag="OO572_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6660188.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33914..34324)
                     /gene="psiE"
                     /locus_tag="OO572_001110"
     CDS             complement(33914..34324)
                     /gene="psiE"
                     /locus_tag="OO572_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6660189.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34450..34593)
                     /locus_tag="OO572_001111"
     CDS             complement(34450..34593)
                     /locus_tag="OO572_001111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660190.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34594..34872
                     /locus_tag="OO572_001112"
     CDS             34594..34872
                     /locus_tag="OO572_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660191.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34919..37015)
                     /locus_tag="OO572_001113"
     CDS             complement(34919..37015)
                     /locus_tag="OO572_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6660192.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNNLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37015..37752)
                     /locus_tag="OO572_001114"
     CDS             complement(37015..37752)
                     /locus_tag="OO572_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6660193.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37749..38387)
                     /locus_tag="OO572_001115"
     CDS             complement(37749..38387)
                     /locus_tag="OO572_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6660194.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38501..38743)
                     /locus_tag="OO572_001116"
     CDS             complement(38501..38743)
                     /locus_tag="OO572_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660195.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39206..40855)
                     /gene="pgi"
                     /locus_tag="OO572_001117"
     CDS             complement(39206..40855)
                     /gene="pgi"
                     /locus_tag="OO572_001117"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6660196.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41380..>42503
                     /gene="lysC"
                     /locus_tag="OO572_001118"
     CDS             41380..>42503
                     /gene="lysC"
                     /locus_tag="OO572_001118"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6660197.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELS"
BASE COUNT        10580 a        10953 c        10417 g        10553 t
ORIGIN      
        1 gaggcttctt ttttatggct ggcagatgct ttaatcatcc accttaaaac aatataacct
       61 attgttttaa tgacaaatca gaacggaatg tcatcatcaa agtccatcgg cggctcgtta
      121 gacggcgctg ccggagcgga ctgctgcggg cgagactgcg cgccgccgct gaactgattg
      181 ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc accgatattg
      241 ccacctgccg gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg
      301 ttcaccacga cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta
      361 cgcagctgac cttcgatata aacctgagaa cctttacgca gatattcgct cgccacttct
      421 gccagtttgc cgaacagcac aacgcggtgc cattcagtct gttctttcat ctcgccggtc
      481 gctttatcac gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt
      541 ggcatgtagc gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg
      601 cctctgctgg ccatgttcgt gtctcctgaa aaaaatcgtt ctgaataagt gtaaacgcgc
      661 gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg ggaaacaaac
      721 ctggccagac attgttacac aacactccgg ataatgcatt ccaatactgt atattcattc
      781 aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg ccaccgggca
      841 aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg cgcccgcacc
      901 cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt cgtgaccggg
      961 ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga agggcagcgc
     1021 cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat ggaaaagccg
     1081 gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa atcgacgtct
     1141 cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt gcgtttgttg
     1201 ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc ggcgcaaacc
     1261 gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct gatgctgctc
     1321 gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa cctggcaagc
     1381 cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga tccgccgaaa
     1441 ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa ggtgcgtgac
     1501 gatcttaccc aacgtcttgc ggagtcgttt gaaaccgcgc tggaactttc cggtggtaca
     1561 gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc cgccaacttc
     1621 gcctgcccaa tttgcggcta cagtatgcgt gaactggagc cgcgactgtt ttcgtttaac
     1681 aacccggcag gtgcctgccc gacctgtgac ggccttggcg tacagcaata tttcgatcct
     1741 gaccgcgtga tccaaaaccc cgagctgtca ctggctggcg gtgcgatccg tggctgggat
     1801 cgccgcaact tctattactt ccagatgctg aaatcgctgg cagatcacta taagttcgac
     1861 gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt gtacggttct
     1921 ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc catccgtcgt
     1981 catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac agaatccagt
     2041 gcggtacgtg aagaattagc caagtttatc agcaatcgcc catgcgccag ctgcgaaggg
     2101 acgcgtctgc gtcgggaagc gcgccacgtt tatgtcgaga atacgccgct gcctgctatc
     2161 tccgacatga gcatcggtca tgcgatggaa ttcttcaaca atctcaaact cgctggtcag
     2221 cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgcctgaa attcctcgta
     2281 aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacactttc cggcggtgaa
     2341 gcccagcgta tccgtctggc gagccagatt ggtgcgggcc tggttggcgt tatgtacgtg
     2401 ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt gggtacgctt
     2461 atccatctgc gcgatctcgg taataccgtg attgtggtgg agcatgacga agacgcaatt
     2521 cgcgccgctg accatgtgat cgatatcggt cctggtgcgg gtgttcacgg cggtgaagtg
     2581 gtcgcagaag gtccgctgga agcgattatg gcggtgcctg aatcgttgac cgggcagtac
     2641 atgagcggta aacgcaagat tgaagtgccg aagaaacgcg ttccggcgaa tccagaaaaa
     2701 gtgctgaagc tgacaggcgc acgcggcaac aacctgaagg acgtgacgct cacgctgcca
     2761 gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac gctgattaac
     2821 gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc cgaaccggca
     2881 ccgtatcgcg atattcaggg actggagcat ttcgataaag tgatcgatat cgaccaaagc
     2941 ccaattggtc gtactccacg ttctaacccg gcgacctata ccggcgtgtt tacgcctgtg
     3001 cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgtg gttatacgcc aggacgtttc
     3061 agctttaacg tccgtggcgg gcgctgcgaa gcctgtcagg gcgacggtgt gatcaaagtg
     3121 gagatgcact tcctgccgga tatctacgtg ccgtgcgatc agtgcaaagg taaacgctat
     3181 aaccgtgaaa cgctggaaat taagtacaaa ggcaaaacca tccacgaagt gctggatatg
     3241 accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg taagctgcaa
     3301 acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac cacactttct
     3361 ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg caccgggcag
     3421 acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat tcagcaactc
     3481 ctcgacgtgc tgcataaact gcgcgatcag ggcaatacca ttgtggtgat tgagcacaat
     3541 ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg cggcagtggc
     3601 ggcggcgaaa tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga agcttcgcat
     3661 acggcacgct tcctcaagcc gatgctgtaa tcgttaaggc cgctttctga gcggcctttt
     3721 cctttcagag ttgtaccagc aatttacgct tttcttccgg tagtaaattc actgcctgct
     3781 gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg tcgagataca
     3841 cggtgctcca gtgcgcttta ttcagatggc ggctaggacg cacatcgctg tgctgctgac
     3901 gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga ttttctactt
     3961 ctttcaccat cgcaaacagt acatcttcca ctttgatctg cgtcgctttc cagtcgttat
     4021 gcacgctctg ttctgcgctt ggttttgcca tgcaatattg tagcaactcc gaaatggtca
     4081 ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg atgcgatgtt
     4141 cccccagcca gatgccttgc caagtgccgg tctgaatacg ccctttatgt accggcaata
     4201 caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct gctccctcat
     4261 agtcatgctc ataatttccg ttgtcgggaa cggtgcggag gaaaaaacac tccatgtcgt
     4321 ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta tgttgcagca
     4381 acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc agaatttcat
     4441 ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt tgataccaca
     4501 tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg aaaggataaa
     4561 ggtgtgatta aaaaaacagc attcgggaga gcatcactct ctcccgctct gtcagtattc
     4621 tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt tgtaggtaga
     4681 gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg cggtaatatc
     4741 gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt gcgattttgt
     4801 attttgccct ggtttatcgc ccgcaaagat taccggattc atgttggtgg caggaatatg
     4861 aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac gaccagtcac
     4921 aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag cgacctcttt
     4981 tggaatgctg aattcatccc agccattatt cattttttcc cagaacacag gatttttcag
     5041 ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaccccg gactggaaaa
     5101 aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg cgaggctatt
     5161 ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc tggcaacgtt
     5221 agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac tgtttagagc
     5281 gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt tccctaaata
     5341 gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg accataacgc
     5401 cagctgccgg ggaaaggaag gattatctgc ggtttttgtg atgaaaagca ggatgattat
     5461 atttaaccca gtaaatacgt tcctgtcttt ttttgtataa aaaatgttta ttactcgatg
     5521 taaggataca ttaattttac gtttattatg atgaatcctc ggcaaatgag cgaaatttga
     5581 tagaacattt atctgctgta cgatttaatg aacttaatga tacagcatta caaaaacaat
     5641 cgaagtttat aaagatgatt tctgattgac cagcccccta ccctacaatg gtgattaatt
     5701 ttattactcg cgatggagat gatgatatga atatatcata tgtgaatagc aacaaaacga
     5761 catctttacc tgttgagcta gatgcactaa ataataaaga tatctcttat gccaaagatt
     5821 tctttttata catagaaaca cagttaaaaa ttgcaaaaga tttttgtaga cctggagaag
     5881 aagtatcaag ttctattgca agtaaagttt ttcacgcatt tattgattta gttaacaaaa
     5941 tcaggggtaa gaaagacttt atgtatattt gcacgctttg ctgttttgcc gaggaggtta
     6001 aaggtgatta ttctcattac aggacctttt tatttgatat tggtaatcaa tataaggtta
     6061 aacttacaca aagcggaaaa aagagttctc tttaacatta gaatttaacg atactataat
     6121 tgaatctcag aaagtcacag gcaataaggc aaagcatatt cttgaggata tagaaatatt
     6181 ctatcgtaac aaacccgata cttattatta aatttaataa tggttagatg tgcagaaatt
     6241 attactccac acgaaaaaat agggaaagga attccagata attgggataa acttaacaat
     6301 aaaagaactg acaacgtttt attatctctc acgtagaaca acggcatcaa aaaaatcatc
     6361 tacaaagaaa tagtgaaagc gacgacagtt taatttcact gcaggctggg taactccagc
     6421 ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg
     6481 tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac
     6541 gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa
     6601 gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc aattcctgac
     6661 gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg
     6721 cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt
     6781 tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac
     6841 acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat
     6901 tgctcaccag agcgggtaat ccagcactgg caatggcgcg aatagcgtag gcatcctctt
     6961 ccataccggc actaaatcct tgataggcaa tatcgaggaa tgggataagc tcgcgggctt
     7021 tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt
     7081 ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca
     7141 ggtcattaaa gcgcacgctg ttagtcactt cgtcatacca ggggtaagta ctcacttcga
     7201 atccagcccc ggcgaatatt gctacgtggt tttcccaagt aggatcgctg acccagacgc
     7261 ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc
     7321 caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc gcaccaaaca
     7381 gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag
     7441 cgccatgggg ctgcgcattc aggcgcgctt ccgcctccgc cacggctttc aattgtggaa
     7501 taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt
     7561 cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt
     7621 gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag
     7681 aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactaa
     7741 tgataaacgt acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt
     7801 attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca
     7861 atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg
     7921 taataagttc gtaagcgctt actttcgtca tttcagcgat acgttctacg ggcaaacctt
     7981 cgccccataa aatgaccgga tccccggctt tgtcctgcgc ctgtggacct aagtctacgc
     8041 agatcatatc catcgccacg cgcccgacaa tcggtacttc gcgaccgttc accagcactg
     8101 gcgtaccgga cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa
     8161 gacgggtatc acgttcgctt acccaggttc caccataacc aacaggctct ccggctttat
     8221 gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg
     8281 caccggtgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc
     8341 agtcaaaatg cgactgtggc cacagcagaa tgccacccga cgcggcaatg gaacgttgac
     8401 caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt
     8461 ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc
     8521 actgggtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca
     8581 taccggtatc gagtttcatc cagacggtaa ccggctcgtc caagctagcc tcttccagcg
     8641 cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca
     8701 gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac
     8761 gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc
     8821 gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac
     8881 tggcaggtgc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca
     8941 caacagttgc cgcttgcatt tgtgttcctt gataattgtt tgctttaatt acctaattca
     9001 taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat
     9061 tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata
     9121 atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac
     9181 atgatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccgga
     9241 cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg
     9301 ttcagttctt tcgccagtgc tttcagcgag cgggagattt ctgcaatttc cagcgtacgg
     9361 ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg
     9421 ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg
     9481 gaagagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg
     9541 cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac
     9601 agcgacgcca gagaacgcat catgatctgt tctgatggca tctccagcga gaagataagt
     9661 actggtttat cctgcaacat tgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta
     9721 cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt
     9781 ttgttgagat cgtcataacc ggtgtttacc ccggtaacgc catcgtgtgg ctgctgaaac
     9841 aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg
     9901 tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag gtccagcaga
     9961 tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt cgcaaccgag
    10021 atcatctcac ggacaacggc acgttcacgc acgatgtccg cataagcact gatgttcgcc
    10081 gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt
    10141 tgcccctggc gttccaacga ttccgcaagg gtaatcagat cgataggact accgctttcc
    10201 tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc gggtgtaaaa atcgtctgct
    10261 accacacgct cggctacatc atcccagcgt tcgttatcca gcattaaacc gcccaacacc
    10321 gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt
    10381 tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt
    10441 accgaaataa agaatgggtc gaaactttac catatgaagc agaccctgac gatacgttct
    10501 ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa
    10561 gccgtagagt tcactcctgc cgatccggcg gagaatgaaa tccaggtcga aaataaagcc
    10621 atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc gccatcgcta
    10681 cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaag
    10741 catattaagg ctggcgatca tgtagtctat gcgcagtcgg cgttaggcgc ttacagctct
    10801 gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa
    10861 gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa aacctatgaa
    10921 attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcattgg cttaattgcc
    10981 tgccagtggg caaaagccct gggcgcgaaa ctcatcggca ccgtaggaac cgcgcaaaaa
    11041 gcgcagagcg cgctaaaagc cggcgcgtgg caggttatta actatcgtga agagaatctg
    11101 gtcgagcgat tgaaagagat caccggcggt aagaaagtgc gcgtagtgta cgattccgtg
    11161 ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtcagt
    11221 tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcaaaaaggc
    11281 tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc
    11341 gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc
    11401 gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg
    11461 gcgacgcaag gttccagcct gctgattcca taaaagaaat agggcttcca cctgggaagc
    11521 cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc
    11581 aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt
    11641 gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt tcggcatttt
    11701 tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag
    11761 caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag
    11821 cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat
    11881 cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca
    11941 agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag
    12001 ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga
    12061 gcgcgtgttc cagcacgtta atgtcagcac ccgctttatg ggcattttca cttaaataac
    12121 gccgccactg ccgcgcgcca ggaataccct ggaacaaacc cagcatatgc cggataatat
    12181 ggccgagata cgtcccctgg ctgagttcat gctcaatgta cggatacatg gcacgcacta
    12241 ctgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct accgccgcca
    12301 gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaaat
    12361 gcgctttagc ttcttccagc gacttgatac caccgttaat cgacattgtc agatgcggga
    12421 agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca cggttttctt
    12481 tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gatgaacatc tcacactcgc
    12541 ctttgccgga aacggtattg atgaaatcgc agagaaattc atagctgtcc tggtcgtcga
    12601 tgccaatacg cgttttcacc gtcaccggaa tcgacaccac atcgcgcatc gctttcacgc
    12661 agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc
    12721 ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccgcgcgct tctgccagct
    12781 ttgcacactg cgccagcgcc gccggatcgc taccgcccag ttgcaacgct accggatgtt
    12841 cttcttcact gtacgccaga taatcacctt taccgtgaat tatcgcccct gtggtcacca
    12901 tttcggtata aagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt
    12961 ccgtccagtc gagcattggt gcaacactaa accgaccact ccagtgaacg tcagtttttt
    13021 caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat
    13081 gtagagatta ttttatcgat atatcatggg gttatgttat tcagcttcac tgttcaggag
    13141 gctcaatagc ggggtactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa
    13201 attgagatcc tctcaaggca tgaagctctc acgaagtgat ggaaataatc ttattagccg
    13261 ttaactttgt taaggccaat gataaacaat ccaggttcga cgataaataa aaaatcacac
    13321 attaaactct ggtgatatat ctccctgcta atagcattga tagagaaaaa agaacccaat
    13381 aaatattggg tccttttata taatgccctc catactatcg aagaacttca catattattt
    13441 ctccgattta accccgaaca agtcataaat taatttagaa gtgtctgtaa gtattttaat
    13501 ctcttccttt gaggttgggt caaacgactt cgcgaagcta attaattgtg gtgcagcatc
    13561 tcgcattttg cttaaaatat caggatcgag cgttccttca ttcaccagat gtgacatctt
    13621 atccagatag ccatcaaaat tcatttctcc cccatctgcc agacgcttca tctctcccag
    13681 gtacttcttc atatcacgtt gatataattt ttcaaactga gctttcaggt agctctcatt
    13741 attttcatta atatacactg tttctgataa atctgcgtag gcactagaat atgaaagtga
    13801 aattaacagc gaagcaaata attttaagct gttgtcatca tgttcctggc aggcattaac
    13861 aaacgttaaa aaaccagaac ctattgcttg aaaatgtata ttcgctaaag gttcattatc
    13921 cttagattcc tcataaaata gctgaaccgt ggagggaagg ccattcttag tttctacatc
    13981 aaaagtacat tgttcaatgg gcattgcatc tttattatcc ccaggattac atatactatt
    14041 aatcgcgaaa tgaaaaatag cccggtcaac aactgatgca ataaagtcac gatcgtttaa
    14101 atcctcatca gtgcaatcgt cagtatactt attgacccac attttgagat catttatgtg
    14161 ctcaattaaa aacacatttt tttacgtgca tgccataaat tatgcgtgat tcgttgaata
    14221 aacaaatcta tttttgcaat aattatatcc cgatgacgca gcgctgcata atcaatagat
    14281 ttaccatata tattatttaa tttacctttg attgaacctc tattatccaa tttatctttg
    14341 gttgcatctt tattttcgaa ttgatttgaa ttgagtgttt tattaaagta tccagttgca
    14401 ccgggtatta tcttcaacat gattcatcta ccttttttgt cgtaccgtgt cctccttata
    14461 attcttttgc catctggtca ctatcaaaaa tcgctcataa tttctgaact acaggtatgc
    14521 gtttaaaccc agttaaataa atagcgagtt aacttgaagc aatttattta aaaatgaatt
    14581 attaattatt atcgtgttga taatggtacg ataaggcaac acaacacctt acctacaaaa
    14641 cagaaaatca acattacggc aacaataagg gttctcggtg tttttcctgg aacatggtaa
    14701 agtaaggaca ttcttaaccc ccactttgag gtgcccgatg gaaaagacca caacgcagga
    14761 gttattagcg caggctgaaa aaatctgcgc gcagcgtaat gtgcgcctga ccccacagcg
    14821 cctggaagtg ttgcgcctga tgagtctgca ggatggcgct atcagcgctt atgatctgct
    14881 tgatttactg cgcgaagctg aaccgcaagc caagccgcca acggtttatc gcgcgctgga
    14941 ttttctgctt gagcaaggtt ttgtgcataa ggtggaatcc accaacagtt atgtgctctg
    15001 tcatctgttc gaccagccca cgcatacgtc agccatgttt atttgcgatc gctgcggcgc
    15061 ggtgaaagaa gagtgtgcag aaggcgtgga agacattatg catacgctgg cggcaaaaat
    15121 ggggtttgcc ctgcggcata atgtgattga agcacatggg ctctgtgcgg catgcgtgga
    15181 agtggaagcg tgtcgtcatc ctgaacagtg ccagcatgat cactctgtgc aggtgaaaaa
    15241 gaaaccgcgt taagagggcg tacatccttg tacacgtcgg gcaggaggga ttaattacca
    15301 gcgatattca ttgcgggttt cccaatccac gacctctttt tctgcctgat ctttctgata
    15361 accataacgt tcctggattt taccgaccag ttgatcacgt ttaccttcaa tgatcgtcat
    15421 atcatcatcg gtcagtttgc cccattgctc tttcacttta cctttaaact gtttccagtt
    15481 accgccggct tcatctttat tcataatcaa gacctcatcg ttaggttgtg aatgagagta
    15541 cgttcacttt tcttctgaac gtgagattaa gtgtagtcag caatttggct taggataatt
    15601 tattcagaat ttttaaccgt cacgttgcgg caaaccaggt accgttgcgc cagtgacgcc
    15661 gccagatagc cgccagagaa agcccgcgca acgccagaaa gacggttaat gccagccaca
    15721 aaccatgatt acccagccac ggcagcgtaa ggagcgtcag cgcaaaacct gcggcggcca
    15781 ccgccatact gttacgcatt tcggcggcac gcgttgcgcc tataaacatg ccgtccagca
    15841 gataacacca gacgccaacc aacggcaaaa tcacctgcca gataagatag cggtcagcca
    15901 gctgctgaat ctgggttaac gacgtcagca acgcaatgat gtgttcccct gccagcaaat
    15961 aaaccaccga aaatagtaac gctacaatcc ccgactggcg gcacgctgcc cgccatacat
    16021 ccagcaactg gctaccgtcg cgcgcaccgt atgcctgacc ggagtgcgct tcaaccgcgt
    16081 aggcaaaacc atcaagcgca taggcggtaa aggtgagtag cgtcatcaga accgcgttaa
    16141 cagcgataat gtcactcccc agtcgcgcac caagtacggt gatcgcgccg aaacagagtt
    16201 gcaacaacag cgaacgcagc atgatatcgc ggttaagcgc cagcaagcga cggaagtttc
    16261 ctcgccaggc agttttcagc atttcgccgg aaattccgcg tagtttgagg attttacgca
    16321 ccattagcag accaatcagc aatgttgcat attccgcaat aaccgtcgcc agcgccgcgc
    16381 cctgcacgtt catatgcagc cccatcacca gccagacatc cagcacaatg ttgaggatat
    16441 tgccgaccac taacaaaatt actggcgcac gggcatattg cacgccgagt aaccaaccga
    16501 gtaataccag attcgccagc gacgccggag cgcttaacca gcggatttca agaaagcgcc
    16561 gcgcctgttc caggaccgct tcactaccgc caacaatatg cagcgccaga tcgataatcg
    16621 gcgtacgcaa cagcgcaatt aacgccccgg cccccaacgc caacagcaac ggttgcacca
    16681 gcgcacgggc taatgcctga ggatttttgg caccataagc ctgcgcagtc agcccggtgg
    16741 tgctcatgcg taaaaacagc aacagcataa agagaaagct ggtcgccgtt gcaccaaccg
    16801 ccacgccgcc caaataaacc ggactatcaa gatgaccaat taccgccgta tcgaccagtc
    16861 ccagcaacgg aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc cagagtgctt
    16921 tatcagatga agtgaggaat gccatgcaga caagcctgat gaagagagat gaaaaacaaa
    16981 ccgcgacgcc aagcggcatc gcggtctcag agatatgtta cagccagtcg ccgttgcgaa
    17041 taaccccaac cgccagccct tcaatggtga agctctgctg acgaaggtca acgacaattg
    17101 gtttaaactc gctattttct ggcaacagtt cgactttatt gccctgtttt ttcaggcgct
    17161 taacggtaac ttcgtcatca atacgtgcga caacgacctg accgttacgt acatcctgag
    17221 ttttatgcac tgccagcaag tcgccatcca taatgccgat atctttcatc gacatcccgc
    17281 tgacgcgcag caggaaatca gcattcggct tgaacaagga aggatcgacc tgataatgac
    17341 cttcaatatg ctgttgcgcc agaagcggtt caccggcagc cacacgacct accagcggca
    17401 acccttcttc ctcttcctgc aacagacgaa tcccgcgtga tgcgccggaa acaatttcaa
    17461 taacgccttt gcgtgccagc gccttcagat gttcttcagc cgcgtttggg gaacggaacc
    17521 ccaaacgctg cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg atgtgatcac
    17581 ggatgagatc aaacacctct tgttgcctgg ccgttaacgc tttcattccg ccccctgggt
    17641 gtatatacag ttatactgtg agtatataca gcaaaaggcg attttggaac cataaactgc
    17701 acaataaacc agagatttat cgaaatctgg aagggttatc caaaatgcga ccataacagg
    17761 atgcaccagg taatcacggc gacgataatg gcaatcagca ccgcggcgga tcccatatct
    17821 tttgcgcgtc cggaaagctc atggtattca gagccaattc ggtcaaccac tgcttcgatg
    17881 gcgctattga ggatttccac aatcatcacc agcatcacgg agctgataag cagcacgcgg
    17941 gtaattgcgt ccacatccag ccagcaggcg atgaccaccg ccaacaatac cgctacgcct
    18001 tcctgacgga atgccgcttc gttgatccat gcagcgcgta aacctttcca ggaatagcca
    18061 gcagctttga taattcgggt gaatccagtt gtattattgg ccattgaaag aaccttttta
    18121 cattatgagc gtcaatatca gtgtaccgtt aaatccgatg attcgggttt gcgtaacgcc
    18181 gcgcgaaaca tgagcggata ccacagaatt tcccatgact ttctgctatc cttgccgcgc
    18241 atttgcatta ttaaccagag gctttacatc gtttatgtcc ggctggccac gaatttacta
    18301 caaattactg aatttaccat taagcatcct ggtaaaaagc aagtctattc cggcagatcc
    18361 tgccccggaa ctggggctgg atacctctcg tccaattatg tacgttttac cgtacaactc
    18421 gaaagcagat ttgctgacgt tgcgcgccca gtgtctggca catgatttgc ctgacccgtt
    18481 agagccgctg gaaatcgacg gcacgctact gccgcgctat gtgttcattc acggcgggcc
    18541 gcgtgtattc acctattaca cgccgaaaga agagtctatt aagcttttcc acgactatct
    18601 cgatttgcac cgtagcaacc caaatctgga tgtgcagatg gtgccagtat cggtgatgtt
    18661 tggtcgcgcg ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta tgcttaacgg
    18721 cgtacagaaa tttttcgctg tactgtggct cggtcgcgac agttttgtgc gtttctcgcc
    18781 gtcagtgtcg ctgcgccgta tggcggatga acacggcacg gataaaacta tcgctcagaa
    18841 actggcgcgc gtggcgcgta tgcactttgc ccgtcaacgt ctggctgccg taggcccacg
    18901 tctgcctgct cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca ttgccaaagc
    18961 ggtagaagat gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc agaacgcgat
    19021 tgcgctgatg gaagagattg cggcgaattt ctcttacgag atgatccgcc tgactgaccg
    19081 cattctgggc ttcacctgga accgatttta tcagggcatc aacgtccata acgccgagcg
    19141 cgttcgccag ctggcccacg acggtcatga gctggtgtat gtgccttgcc accgcagtca
    19201 catggactac ctgctacttt cttacgtgct gtaccaccag ggactggtgc cgccgcatat
    19261 cgccgccggg atcaacctga acttctggcc ggccgggccg attttccgcc gtctgggggc
    19321 gttctttatt cgccgtactt ttaaaggcaa taaactttat tccaccgttt tccgtgagta
    19381 tctcggcgaa ctgttcagcc gtggttattc cgtcgagtac ttcgtggaag gcggtcgttc
    19441 ccgcacgggg cgtttgctgg atccgaaaac cggtacgctg tcgatgacca ttcaggcgat
    19501 gctgcgtggc ggcacgcgtc cgattacgct gattccgatc tatatcggtt atgagcacgt
    19561 catggaagtg ggtacttacg ccaaagaact gcgcggcgcg acgaaagaga aagagagcct
    19621 gccgcagatg ctgcgcggtt taagcaagct gcgtaatctc ggtcagggtt acgtcaactt
    19681 cggtgaacca atgccgttga tgacctacct taaccagcac gtaccagact ggcgtgaatc
    19741 tatcgatccc atcgaagcgg tgcgtccggc ctggttaacg ccgacggtca ataatattgc
    19801 tgccgatctg atggtacgca ttaacaacgc aggcgcggca aacgccatga acctgtgctg
    19861 tactgcgcta ctggcatcac gtcagcgctc actcacccgc gagcagttaa ccgagcaact
    19921 caactgctac ctggatctga tgcgcaacgt accttactcc acggactcta ccgttccttc
    19981 agccagcgcc agcgagctta tcgatcacgc gctgcaaatg aacaagtttg aagtcgagaa
    20041 agacactatc ggcgacatca tcattctgcc acgcgagcaa gcggtgctga tgacctacta
    20101 tcgcaacaac attgcgcata tgttggtgct gccttcgctg atggcggcaa ttgtcaccca
    20161 gcatcgccac atctcccgcg acgtattgat ggagcacgtc aatgtgcttt acccaatgct
    20221 gaaagcggag ctgttcctgc gctgggatcg cgacgagttg ccggacgtta ttgatgcgct
    20281 ggcaaatgag atgcaacgtc aggggctgat taccctgcaa gatgatgagt tgcatatcaa
    20341 cccggcgcat tctcgcaccc tacagctgct ggccgcaggc gcgcgcgaaa cgctgcaacg
    20401 ttatgccatc accttctggt tgttgagtgc caacccgtcg atcaaccgcg gtacgctgga
    20461 gaaagagagc cgcaccgttg cgcaacgtct ctccgtgctg cacggcatca acgcgccgga
    20521 gttcttcgac aaggcggtgt tcagttctct ggtgctgacg ctgcgtgatg aagggtatat
    20581 cagcgatagc ggcgatgccg aaccagcaga aacgatgaag gtttatcagt tgctggcgga
    20641 gttgattaca tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg aagggtaatc
    20701 agagagaatt gccggatgcg gcgaaaacgc cttatccggc ctaccatgac ctgcaaattc
    20761 aataaattgc gattcaccgg gtaggcctga gaagcgcaac gcatcaggca acccagaaga
    20821 aagccggatg atcatccggc ttttttacat catcagaaat gccagtaact cattgccagt
    20881 cctaaaaaca gtaccagacc aacatagtta ttattcataa atgctttaaa gcaggcttca
    20941 cgctcgcggt tggcaatcag tttttgttga taaacaaaca gcgcgccagc caccacaatt
    21001 gaccagtaat atccccagcc taagccattt aactcaccga tgatcgccat cagtgccagt
    21061 acgccaatct gcaaaatacc aataatcaat ttatcgtatt ggccgaacag gattgccgtg
    21121 gatttaatgc caatcttcac atcatcatcg cggtcaacca tcgcatactg cgtgtcgtaa
    21181 gccaccgccc agagaatatt ggcgaggaac attaaccagc aactcaatgg caccgactca
    21241 ctcacagcgg caaaagccat tggaatcgac cagccaaacg ccgcgcccag caccacttgc
    21301 gggagatggg tataccgctt cataaacggg tacacccacg ccaatgccag agcggcaatc
    21361 gacaacagaa tggtcatcgt attcagcgtc agaaccagta aaaacgaaat cagtaccagc
    21421 actacaaaca gcgcgcgcgc ctctttctct gttaccgcgc cgctgggaag tggtcggttc
    21481 gccgtgcgct taacatgacc atcaaacttg cgatcagcat aatcattcac cacgcaacca
    21541 gcagcgcgca tcagccagac acccgcgaca aacaccgcca tgatccagag ctgaggaacg
    21601 cccggtgtcg ccacccacaa cgcccataat gttggccaga gcagcagtaa cgcgccaatt
    21661 ggcttatccg tacgcattaa gcgatgaaac gccagcagct tattctgcgt cagactccac
    21721 tccatatttt tttcctctta gtacaacggt gacgccggta aaaacagttc tgttagcaac
    21781 agcggtttac cgcttaatcg caggcgggaa cgtcgccccc acagtccggc atcacggcct
    21841 atctcgataa aatcccgggt tagtgtcgat gatgtgaaca gatagcgtcc taacggcgtt
    21901 ttacccaatt tttgtaacgc tagttccggc ccgcttaatg ttgatacagg aacgacggta
    21961 cgaccggcaa gccacggttc accatcggca cataacaaaa tttcacgtaa ccagtaacga
    22021 gactctttcg gcagcagcgg cagttcttcg gggatttcat tctgctcgac aaacccctcg
    22081 cggatcatcg tcacactcac cgtttttccc tgctgttcaa aacgttttgt catggaatcc
    22141 tccagcaaca gccagtcgag cagttgcgga tccagggcag ggatctcttt aaaatagcgc
    22201 agcgcacgca gttgcgttaa cgcggggtgt gacatgccga actctccgtt acataacgta
    22261 aaggcattgt atcgcagaaa agtgtttacg ggagatgtaa agcaaattaa gtgatctaat
    22321 gcgcaacaaa aacgcaacaa tcgcatgaag tggtaaaacg ctgttgaacc ttacgctttg
    22381 ccgatgcccg tgctggcgag ggcaaccata ggggcatcac cgcccatgat gctaggaacg
    22441 gtgatgtttt cctttactta aataccttta ttctttatac gagtagctcg ccaactcgca
    22501 gtaaaaattg ggaaaagcgc atatttacca tgagatttgg agctgaaaag tgtttcctga
    22561 gtaagattaa taggcaacat cttatgtctg gatttataga tacacgtgga attccaattg
    22621 gtatcttttg taggattcaa catatcatgt aagcgctggg agcttaccac cataacaggc
    22681 ccatcatcga cagaggagaa caggataaat atattatcgt ttcgtgttaa tttattagtg
    22741 ttaaaggttc ttaacttttc ttcagtttct ataaatatag gtgaaacatc atcactacga
    22801 atccactctt tataaagata ccaggcattt gctttgatca tatcatccgt tgatcgtaag
    22861 gcctgatcga taagttggat aaaaaaattg ttatttttat caaccagccc tttattcata
    22921 ttaaatctgt taataaaatg gatcatctgt tgggtatcga aattcacctt actgaatcga
    22981 tgttgacgca taaaattatc gaacttgtca tcttccccag aatgtatata actacgtata
    23041 attggatctt ttatataagg acaatggcta aatgttttct tcatattctc aataaagttt
    23101 ttattaacaa tattgagaat ttttaattgc atcaccagtt ttactgccag aacggatttc
    23161 aatatcattg cattatctgg caatgtatca atcattttta taatgttatt gatataacca
    23221 tcgagatctt cattcccaaa tatatctgaa taatcaatag ttaattccaa ttgttctgta
    23281 atgcaatctt aaaaattccc tgtcgacatt cgcacatgat caaacataac agctgtcaaa
    23341 ttcgatttaa taaatgatat ttctttggca gacagaaact ctgctctggt gaagtgcgaa
    23401 gcgtaaagat aacaattatc gaagtttgaa tttctaatct gacattcgcg aaaattacta
    23461 tgaccaagtt gactctgcga gaagtcaact gtatcaagaa tattttggct aaaatcaaca
    23521 cattcgccac tcgttttgag aaaaatcaca ttgtgtaaac gtcgacattt aaatatgaca
    23581 ttatcaactg ttgccttagt aaaattgcaa ttctcaattt ctttacattc gaaagttaca
    23641 tgttgtagat ttgatgataa aaaagacacc ttagaaataa cgtcaccttt aaaatgacaa
    23701 tttgttaaat caaaatcaga caggtcgata tacccctgcg catctttatc catcagaaaa
    23761 ttacaagcgc attctatttt ttcatttctg tagaatttta cacttatagt atcattacta
    23821 attgagactt cacgtaacat atttattgca tgattttttt cgttccgttg ttttatattt
    23881 aaaaatatat cactgatata aataaagtaa tctctgttta aacctgtcac cccattgaaa
    23941 ttacgtagta aatcaagatc tactatatta tttccttgca ccacatcatg tttcatcaaa
    24001 gagttattgc cgccagaatt tgtgatatga cctatcataa aacctctgta atacagaaat
    24061 aaaataacct ggaaaagtag cctgagcgtc cagacactac cggcgaaagg aaaagaatct
    24121 ctgataatgc attgaaataa tggacattct tagtgaagaa gaagtgaagt actataaaaa
    24181 atcgctcata tcccaggaag gaagcataaa aaaaggtgcg ccaggagacg caccagttgt
    24241 tgcaaaatca gcactgtggg gcgtaattac cccttgcctt ttacactgct gataaaggtg
    24301 gaacgggcag atgtcgatcc caggcgttca gcttcatcaa gcagttttaa cgccttatca
    24361 acatcacctt tcgcgacagc gtttttgatc gcggtattaa aataactttc cgtgtcgttg
    24421 agcatcggct cgcttttctt caccggcacc ggagcaggtg cagccacagc tggtgccgcc
    24481 gtgttgccta ccgtaaccgg agctggagcg gaagaaccaa atagcggtcc taccaacacg
    24541 ctggagctgg agttcgtttt gactttcagt ttcagtaagc catcgctggt atgacgagca
    24601 accggatcgg gaatatccgg gatcgagtta ccgacgcctt tggcataggc tttagccggg
    24661 tcgagcagtt gggtcgtctg ctggagatct ttttccgtgg taaagaccag aacataaagt
    24721 ttttgctgcc ccaacgccgg tgtcaggcgc ataacgcctt ccagccgatc tgcactcatc
    24781 acgcctagtt cctggtaggt gaaataactg ctggggaaga aggctgatgg ggtcatgttc
    24841 tgatcaagaa tcagcacgtt cggcgcaaaa acgctggttt gtttgttcac ttcgctggtc
    24901 agcgtcaggg tcagttcgcc aatgtttgcc gggacgctgt acgcagcaac cggaccactg
    24961 atgccgggaa cgttcagttg ttggccgccg gtcgccagtt gggtggtctg ggttttagat
    25021 tgatcgaccg gtgtccaggt gagttgttgc agcgcggcag atggaatggc tggcgcgtcg
    25081 ctggtgtttt gcggtacgta gttaacatcg gcaaggctaa ttccaggcgc gcttgccagt
    25141 aaccctgctg ataaacagag ggcgatgaga cttttattca ttttcattgt tttcacctca
    25201 aaatctggag ctcagcggta gccaggcaat agcgcgctaa acccgataat cagaggggct
    25261 ttcgcccctt tcagataatg acaacctgtt tttatgccgg atgcggcgta aacgccttat
    25321 ccggcccagg ttttgctatt accaccagat ttccatctgg gcaccgaagg tccactcgtc
    25381 gctgtcgcca cgaccgaagc tgccgccgtt gaaatcagca ggaacggctt tgccgtagtt
    25441 cgggttaacc ttgctatcgc cgttgtagtc gtaaccccat ttctcatccc acttggcgta
    25501 ggttgcgaag acacgaatag ccgggcgtga ccagatgccg tcgccagcct gccattgttg
    25561 tgcgagggta attttgtact gattgttctt gtcgccggtg cgctgggatt cgacgttgtc
    25621 gtagccgatt tccatcacgg tgctcatgat tggcgtccac ttgtacatcg ggcgaatacc
    25681 aacggtccac cacttggtgc cgttgtcgtt atcccagttg atatcctggt acatacccac
    25741 gtacatcatg tcccagttgt cgcccatgga gatcgcaccg tggtcgagga tacgcagcat
    25801 gtgaccgttg ttgttgatat tgtaggcaaa tttttcgtta tcaaacgcga cgccagaacc
    25861 ctgcgacaga cctttaccct gcgaggtcat cgagtcagta gcgtactgaa caacaaactt
    25921 gttaaagccc ttcaggacac tctgagtatg ttcagcagtg aataaccagc cgtctttcga
    25981 tgcgccatca accagacgat agttatcacg caggttggca cgaccgtagt cgacacccag
    26041 ttctaatgtg ccgcccgggt tgatttccat ctgcgctaaa cgcacatcga aaacgtcgtt
    26101 cgcggtttcg ttggtatagt cataaatatt gttgctggca aaagaggaag aaccaccagc
    26161 ttcagaggag cgggttgctg ccagagagag tttaccgaag ccaacatcga tgttttccag
    26221 accggcacca ggaccagaaa tatcccagta gtagaagtcg atcatatgaa cgtcatgacg
    26281 ttggtagaag cgcttacctg cccagatggt ggaacctggc agccattcga tcaggttttt
    26341 tccctgcacg tttgcttcac ggaaggccgg atcggtagct tcccagtcat tctgttgcgc
    26401 gacggaatag gccacgttag tgtcgaaata gaagctctta tcgccctctt tccacacttc
    26461 ctgacccaat tttaattcag cataagtttc acattcgttg ccaagacggt atttactttg
    26521 agcaccggta gtctggaaac actgttgttc accgccgcta cctgtccagc caataccgga
    26581 acgtgcatag ccgtggaaat caacagccat tgcctgagca gacattacgc ccgctgcgac
    26641 ggcaaccgcc agaggaagtt tgcgcagagt aatcatcatt ctatctcctg agtcattgct
    26701 tttctttttt cacatcacct gtgacaggct ttgtgtgttt tgtggggtgc ttaaacgccc
    26761 ggctccttat gcagtcgacg acatgcagtg ccatcctcac ggaacagatg gcaacgctct
    26821 ggcggcaggc cgatagcgaa tgtggcacct tcttctacca acaccacgtc gttctggcgg
    26881 tacaccaggt tttgacgaat ggaagggatc tggatataga tttgggtttc gttgccgagt
    26941 tgctcgacga cctgaacttc accctcaagg atgacgtcag cgatatcact cggcagtaga
    27001 tgttccgggc gaatacccag cgacatattg gctccaacct ggacatcacg gctttcaaca
    27061 ggcagccaga cttgttgacg atttggcatc ggcagctcca cctgcacttg atcgattgcg
    27121 gtggcggtca cttttaccgg caggaagttc atctttggcg aaccgataaa tccggcgaca
    27181 aaacggtctg ccggatagtg gtacagttcc agcggtttcc caacctgtgc cacgcgaccg
    27241 gcgtccagca ccacgatttt gtcggccagc gtcatcgctt cgacctgatc gtgggtgacg
    27301 taaatcattg tgcggcccag gcgtttatgc agacgggaga tttcgatacg catttgcaca
    27361 cgcagtgcag catcgaggtt ggagagcggt tcatcgagca aaaatacgct tggctcggcc
    27421 accagcgtac ggccaatcgc cacgcgctga cgctgaccac cggagagcgc tttcggtttg
    27481 cgatccagca aatgcgccag ttgcagcact tccgccacct ggttgacgcg ttggttaatc
    27541 acctcttttt ttgcgccagc cagtttcagg ccaaatgaca tgttttctgc tactgacagg
    27601 tggggataga gcgcgtaaga ctgaaacacc ataccaacgc cgcgttctgc tggcggagtg
    27661 tcattcatcc gtttctcacc gatgaacagg tcgccgctgg tgatcgtctc aagcccggca
    27721 atcatgcgca gtaaagtcga tttaccgcag ccagacggtc cgacaaacac cacgaattca
    27781 ccttcatgga tatcgagatt gatatctttc gataccacga cctcgcccca ggctttcgtt
    27841 acattttgca gctgtacgct cgccatgccc ttctcccttt gtaacaacct gtcatcgaca
    27901 gcaacattca tgatgggctg actatgcgtc atcaggagat ggcttaaatc ctccaccccc
    27961 tggctttttt atgggggagg aggcgggagg atgagaacgc ggcttctgtg aactaaaccg
    28021 aggtcatgta aggaatttcg tgatgttgct tgcaaaaatc gtggcgattt tatgtgcgca
    28081 tccccacatt accgccaatt ctgtaacaga gatcacacaa agcaacggtg gggcgtaggg
    28141 gcaaggagga tggaaagagg ttgccgtata aagaaactag agtccgttta ggtgttttca
    28201 cgagcacttc accaacaagg accatagatt atgaaaataa aaacaggtgc acgcatcctc
    28261 gcattatccg cattaacgac gatgatgttt tccgcctcgg ctctcgccaa aatcgaagaa
    28321 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
    28381 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
    28441 gagaaattcc ctcaggttgc ggcaactggc gatggccctg acattatctt ctgggcgcac
    28501 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
    28561 ttccaggaca agctgtatcc gtttacatgg gatgccgtac gttacaacgg caagctgatt
    28621 gcttacccaa tcgctgttga agcgttatcg ctgatttata acaaagacct gctgccgaac
    28681 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
    28741 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
    28801 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
    28861 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
    28921 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
    28981 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
    29041 acggtactgc cgaccttcaa gggtcaacca tctaaaccgt tcgttggcgt gctgagcacc
    29101 ggtattaacg ccgccagtcc gaacaaagag ctggcgaaag agttcctcga aaactatctg
    29161 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
    29221 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
    29281 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtacgt
    29341 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
    29401 cagactcgta tcaccaagta atgctgtgaa atgccggatg cggcgtgaac gccttgtccg
    29461 gcctacaaaa ctgaaacgca tgtaggcctg ataagacgca tcagtgtcgc atcaggcagt
    29521 tgttgtcgga taaggcgtga aagccttatc cgtcctggaa tgaggaagaa ccccatggat
    29581 gtcattaaaa agaaacattg gtggcaaagc gacgcgctga aatggtcagt gctaggtctg
    29641 ctcggcctgc tggtgggtta ccttgttgtt ttaatgtacg cacaagggga atacctgttc
    29701 gccattacca cgctgatatt gagttcagcg gggctgtata ttttcgccaa tcgtaaagcc
    29761 tacgcctggc gctatgttta cccgggaatg gctggaatgg gattattcgt cctcttccct
    29821 ctggtctgca ccatcgccat tgccttcacc aactacagca gcactaacca gctgactttt
    29881 gaacgtgcgc aggaagtgtt gttagatcgc tcctggcaag caggcaaaac ctataacttt
    29941 ggtctttacc cggcgggcga tgagtggcaa ctggcgctca gcgacggcga aaccggcaaa
    30001 aattacctct ccgacgcgtt taaatttggc ggcgagcaaa aactgcaact gaaagaaacg
    30061 accgcccagc ccgaaggcga acgcgcgaat ctgcgcgtga ttacccagaa tcgtcaggcg
    30121 ctgagtgaca ttaccgccat tctgccggat ggcaacaaag tgatgatgag ctccctgcgc
    30181 cagttttctg gcacgcagcc gctctacaca ctcgacggtg acggcacgtt gacgaataat
    30241 cagagtggcg tgaaatatcg tccgaataac caaattggct tttaccagtc cattaccgcc
    30301 gacggcaact ggggggatga aaagctaagc cccggttaca ccgtgaccac cggctggaaa
    30361 aactttaccc gcgtctttac cgacgaaggc attcagaaac cgttcctcgc cattttcgtc
    30421 tggaccgtgg tgttctcgct gatcactgtc tttttaacgg tggcggtcgg catggttctg
    30481 gcatgtctgg tgcagtggga agcgttgcgc ggcaaagcgg tctatcgcgt cctgctgatt
    30541 ctgccctacg cggtgccatc gttcatttca atcttgattt tcaaaggatt gtttaaccag
    30601 agcttcggtg aaatcaacat gatgttgagc gcgctgtttg gcgtgaagcc cgcctggttc
    30661 agcgatccga ccaccgctcg cacgatgctg attatcgtca atacctggct gggttatccg
    30721 tacatgatga tcctctgcat gggcttgctg aaagcgattc cggacgattt gtatgaagcc
    30781 tcagcaatgg atggcgcagg tccgttccag aacttcttta agattacgct gccgctgctg
    30841 attaaaccgc tgacgccgct gatgatcgcc agcttcgcct ttaactttaa caacttcgtg
    30901 ctgattcagc tgttaaccaa cggcggcccg gatcgtcttg gcacgaccac gccagccggt
    30961 tataccgacc tgcttgttaa ctacacctac cgtatcgctt ttgaaggcgg cgggggtcag
    31021 gacttcggtc tggcggcagc aattgccacg ctgatcttcc tgctggtggg tgcgctggcg
    31081 atagtgaacc tgaaagccac gcgaatgaag tttgattaag ggagataaca aaaatggcaa
    31141 tggtccaacc gaaatcgcaa aaagcacgtt tgtttattac tcacctgcta ctgctacttt
    31201 ttatcgcggc gattatgttc ccgctgctga tggtcgtcgc tatctcgctg cgtcagggaa
    31261 acttcgcgac cggtagcctg atcccggagc aaatctcctg ggatcactgg aaactggcgt
    31321 taggttttag cgttgaacag gctgatggtc gcattacgcc accgccattc ccggtactgc
    31381 tgtggctgtg gaactcggta aaggtcgctg ggatttccgc gattggcatt gtggcgctct
    31441 ccaccacctg cgcctacgct ttcgcccgta tgcgctttcc aggcaaagcg acgctgctga
    31501 aaggaatgct gattttccag atgttcccgg cagtactttc actggtcgcg ttgtatgcgt
    31561 tgtttgatcg tctgggtgag tacattccat tcattggcct gaatactcac ggcggcgtaa
    31621 ttttcgcgta tctgggtggg attgcgctgc atgtctggac catcaaaggc tatttcgaaa
    31681 ccatcgacag ttcgctggaa gaagcggctg cgctggatgg tgcgacaccg tggcaggcct
    31741 tccgccttgt cctgttgccg ctgtcagtac cgattctggc ggtggtattc atcctgtcgt
    31801 ttatcgctgc cattactgaa gttccggtcg cgtcgctgtt actgcgtgac gtaaacagct
    31861 acaccctggc cgtggggatg cagcaatacc tcaacccgca aaactacctg tggggtgact
    31921 ttgccgccgc tgccgtgatg tctgcattac cgatctccat cgtcttcttg ctggctcaac
    31981 gctggctggt caacggcctg acggcaggtg gtgtgaaagg ttaaagatgt tgttctgcca
    32041 atgttatgcc gctgcaccct caacttacgt tatcccaact tgtgactatt attcggcgct
    32101 ccacggagcg cctttttttc tttcgtctgc aatctgaatc gttcgccggt taatattccc
    32161 atcatagaac ttattatttt tacgttattt gttttcccac ttacgataat tctctttcgt
    32221 gctctgagtc acggcaatag tattgtttca tcaatttttg ataattatca caattaagat
    32281 cacagaaaag acattacgta aacgcattgt aaaaaatgat aattgcctta actgcctgac
    32341 aattccaaca tcaatgcact gataaaagat cagaatggtc taaggcaggt ctgaatgaat
    32401 acccagtata attccagtta tatattttcg attaccttag tcgctacatt aggtggttta
    32461 ttatttggct acgacaccgc cgttatttcc ggtactgttg agtcactcaa taccgtcttt
    32521 gttgctccac aaaacttaag tgaatccgct gccaactccc tgttaggatt ttgcgtagcc
    32581 agcgctctga ttggttgcat catcggcggt gccctcggtg gttattgcag taaccgcttc
    32641 ggccgtcgtg attcacttaa gatttctgct gtcctgtttt ttatttctgg tgtaggttct
    32701 gcctggccag aacttggttt tacctctata aacccggaca acacagtacc tatttatctg
    32761 gcaggttatg tcccggaatt tgttatttat cgtattattg gcggtattgg cgttggttta
    32821 gcctcaatgc tttcaccaat gtatattgcg gaactggctc cagctcatat tcgcggaaaa
    32881 ctggtttcat ttaaccagtt tgcgattatt ttcgggcaac ttttagttta ctgcgtaaac
    32941 tattttattg cccgttccgg tgatgccagc tggctgaata ctgacggcag gcgttatatg
    33001 tttgcctcgg aatgtatccc tgcactgctg ttcttaatgc tgctgtatac cgtgccagaa
    33061 agtcctcgct ggctgatgtc acgcggcaag caagaacaga cggaaagtat cctgcgcaaa
    33121 attatgggca acacgcttgc aactcaggca gtacaggaaa ttaaacactc cctggatcat
    33181 ggccgcaaaa ccggtggtcg tctgctgatg tttggcgtgg gcgtgattgt aatcggcgtt
    33241 atgctctcca tcttccagca atttgtcggc atcaatgtgg tgctgtacta cgcgccggaa
    33301 gtgttcaaaa cgctgggggc cagcacggat atcgcgctgt tgcagaccat tattgtcgga
    33361 gttatcaacc tcacctttac cgtactggca attatgactg tggataaatt tggtcgtaag
    33421 ccactgcaaa ttatcggcgc actcggaatg gcaatcggta tgtttagcct cggtaccgcg
    33481 ttttacactc aggcatcggg tattgtggcg ctactgtcga tgctgttcta tgttgccgcc
    33541 tttgccatgt cctggggtcc ggtatgctgg gtactgctgt cggaaatctt cccgaatgct
    33601 attcgtggta aagcgctggc aatcgcggtg gcggcccagt ggctggcgaa ctacttcgtc
    33661 tcctggacct tcccgatgat ggacaaaaac tcctggctgg tggcccattt ccacaacggt
    33721 ttctcctact ggatttacgg ttgtatgggc gttctggcag cactgtttat gtggaaattt
    33781 gtcccggaaa ccaaaggtaa aacccttgag gagctggaag cgctctggga accggaaacg
    33841 aagaaaacac aacaaactgc tacgctgtaa tcttcctgtc cagcacgccg cgccatttcg
    33901 gcgtgctgtc tttttactcc cgcttcagcc gtttcgaatt acacagccac agggtgatca
    33961 ccagtaacag gatcgcagcc gagtagatca acacatccag tggcgattta tgatcgacga
    34021 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    34081 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    34141 ccagtccttc taccagctca tatttgctgg tttgttctgg cgcgaacagc acatcagcca
    34201 gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag gccgaggcac agcaggccaa
    34261 gattgagtac ggtctgcaaa atggtggaga taaactccac gcgcggacga gagagtgacg
    34321 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    34381 atctatattt tttgtgtatg ttttgttcaa cctggatttg gagcgcgcct ccaccaaagc
    34441 cggatatacc tacccgtagg ccggataagc cgcgcctggc ggcgcatccg gcaagacaaa
    34501 ccgcaccaaa gccggtaatt caaaaatcac ccattcaggt gattcccagc caccagcaat
    34561 gcgcgaaaaa tcgacaatgt tcctctggaa caaatgaaac gtaacctgat aaaggtggta
    34621 aaaatgaagc cttattttgc tgctttgatg ttatcagtct ctgttcttcc tgcttatgct
    34681 ggtccgctcg gtactgctga taaagccgac ctaccgcaaa gcaatgtctc cagcccaatg
    34741 atggcccagt cgctcaggca accagacctg caaccgatat cgacggacag gaagacagaa
    34801 tgttttcgcc tttacacacc agatcgtaaa ccgggggtga actgcgttcc tgatggctca
    34861 acaggtcatt aagaatgagg gtgccagtga gtgcgaaaac tggcaccttt cccatgactt
    34921 agcggaaatt tacgctacgg tcgctggtca tgtcatacaa ctggaactta cgcccaagtt
    34981 gctgaccacc gtcacgcgtc agcggcgtcc agccaatcgc cgcacggctg cgtgtcgggc
    35041 cagacgagaa gagatccaac ggtaccgaga catacacgcc tttggtgaag tccccttcgc
    35101 cgtactcctc tttcgaaacg ttggtgatcg tggcatagcc acccaccacc acgccgctgt
    35161 caaagcgttt agcgatctcc agcgtgccgc ctttatcccc tgccagatac tgcccgacgc
    35221 tggctttcac caacacgtcc tgggcgaaag atggcgtcca gtaggcggtc agatgtccgg
    35281 ttttcacgct gtagtcggtg aatttcatca tatcttttgc actacgccag tcgcgctgtt
    35341 taacgtagtt ggcatccaga ccaaacgccc agttactgtc cagcggacga tacaacactt
    35401 cggcccccgc accgccaaac atggtttcga gataacctcc atagacctga ccgtagaagc
    35461 cgttgcccag atgctggaag tagttggctt gcaggttatt cacatagaca tcgttctgca
    35521 catactcgcg cacatgggta cgaacgcgcg gtaagtgcga gtcctgcggt ggattggtgt
    35581 agttaaactt gtcgtagttg ttggcaagat ttgcaaacag gctgccggtg gtcagtagat
    35641 ggtccgtcag ccacaaatct gccgttccca tcacgcccag ctgatacatg taaaagtttt
    35701 ccgggccacc gaccgactgg ttcagcaccg gatcgatatg gaaatcaaag cgtgatttgt
    35761 cgatatacca gccctgctcg gtggacttcg gaaccactgg ctcgacgcgt ttgtgcgcca
    35821 gcgtcgtttc gtggcccaac ggctctccgg cgagattatt tttcaggctg gcgacatcgg
    35881 tttccgtcgt cacttgcggc atgttaaggc gattttccgt aatgcggatc gttttgatcc
    35941 catccggcag atcgttcatc acgatccgat tagcacggat gatcccttcg cgcgaatcac
    36001 gatatttcac ctgctcgccg gtaacataca gcgtatcgcc tttcgcctgg atctgcggat
    36061 cggcaagtcc ggcgttgtat ttcagcaacg ttaactgatt cgccaccacc gaatgctgca
    36121 aaatggcatc ctgcggctgc ggttgatatt gcgggcgggc gttatcgatg taggacgggc
    36181 gcagatcgtt aaagttggtg cgcagcgtga cgccaaacat aaaggtgttg ccacgttcat
    36241 agctaaggtt aacgtcggcc caatcggtaa cgcgataaat cgccccgacg ttaaacttac
    36301 ttttttgctc cagcttcccg gcaaaatcct gctgataatt attaccttca tactccagtt
    36361 tcagacgcag cggttgccag ggcgtctggt attccacgcc gccaaacagt gaggcaggac
    36421 cgtggaacat ctggctaccg tcgatagatc ccgcctgttt gtagctgtta tcgcgatagc
    36481 aatatttatc actggctgaa cagagcggat tttttacatt accgctggtg cccagatatc
    36541 cccagcccag gccgagcgta aaatcgaacg gcccccaggc tttgctggca acaagatatt
    36601 ccgcatcaaa cagccccgta ccgccgatat cccgcgcacc aaccgccact tgcggcagcc
    36661 agtaactctc ttcccacaaa cgcagtttga gatcgaaggc tttatcttta tacgtttgat
    36721 cgccagagaa cgcttcgacg ctgctgtact gccgggtgcg cacgtcggtg tagcgcagcg
    36781 ttgtttccag ccacgggaag agttgcactg aagctgagta ataacggtac tgatcgttat
    36841 cgcgatagtt cagactcaac tccccttccc gtgccatacg cgcggtggga gtttgtaata
    36901 atcctacgcc accgaaatcc gactgcgacg gaccaatggg cgcaggatat gtttcgccgt
    36961 agcaagctgt gctaatgccc agcgccagta agctaagcag atgtcttttt ttcattattg
    37021 gggtatccgc tgcgtcagag tctgaagaat gtcggcgtta agggcatcag gcgtctcact
    37081 ccagacggaa tctgcgaggc caacataaat aatgctgccg ggcatcggct ctacgtgacg
    37141 cttgttccag taagccaccg gcgctttttg cgtgcgtccg tccgggtaaa cgacccacgc
    37201 gtagctgcga tccgcaccgc tgagcaggct ctggtcagag agatagctcg ccacgtcgcg
    37261 acctggagtg aatggctgat tgccaggatg gctgataagc ccgaacaacg tgaccgtgga
    37321 cggcggtggt ccgacccaca gcgtgtagtt gccctgcaac ggcgggttac cgcgttcggc
    37381 aacgcggacg atatcgggat caagattgat tttttgcctg cctgtcactt tcaatgcctg
    37441 gatttgctgg cgtaaggcgt taatggcggc ggcatcgtcg gtgctggaat ctgcgccctg
    37501 ttctgccagc cgtgtcagca acgcctgctg ctgacgcaat gctgccgccg ttgccagctc
    37561 ttcgctaatc accgcaccag gccaccagct gtttgccagt cgaggttgcc ctaccagatc
    37621 gattaaatgc tctgcgcccg ttagcgtttt ggcctcactg ctgccattgc tgaacacctt
    37681 gacggttcct gccgcgaaga ccgatgacgc tcccacactc aaaagcaacg cgacaatagt
    37741 ttgtttaatc attgttttgc cgccttgatg agagtggttt tcaccgggaa ataatccgcg
    37801 ccgagatact gttccgactg gcgaatttgc ccttcgctgt cgatccagta gcggttatgc
    37861 cagcgcgcct ggtcggtgga gacttcttcg tccagcacgc gaaccagggt ttcatcgctg
    37921 ccgaccttga cggtatcggt gccatcccat ttgaagactg agcgtgcggt ggcgtagcgt
    37981 acctgctggt actcggtcca gcccatcgtg cgcgtccatg ttgcaccatc aacaatttgt
    38041 gcgggtttaa tcagcgggtc ggcggcaagg ttattcactt caatcaggtt gtcgccgcca
    38101 agcaaagtct tcaccagacg accatgttgt gtgacaagag tggcttgatc ctgagtgacc
    38161 catttttgtt gtccgtcttc agcgaaggcc agcaccacaa acaattgcgg cccgccatta
    38221 agctgcatgt actggctggc gtagggcata ttttgaatat catcgtccgt cagctgtacg
    38281 cctggcgtgc cgaacagact gtcccacagt gaattgccca gctctttagt cgtggctgaa
    38341 caggcctgta atagcaggca gataagaatg agtgcaggtc gcttcacgac tcttctccag
    38401 aggggcagaa taaccacacc ggagtgtggt tatggtaaat cccttcatac ttcaagccac
    38461 agatgcaatg actcaaatta ttttggggat acatacttta ttactgggta ctggtggtcg
    38521 tggttgtggt ggtcccggta ttagaaccat caccgccacc ggttgccgcg agcgcgacac
    38581 ccaccgccga gcttacggtg ctgacgctgg tcgcggagga actccccgcc gaaaccgacg
    38641 ttgctgccga ctctgccgct tcgcccacct gtacaggtgc agcccacgca gaagtcgccg
    38701 caagcgcaga tatggcaaaa atgccataca gaactttttt cataacaatt tcccttcatt
    38761 gaatgaatgg agatttaccc aaaaacattt cgggcgggat cgagtataca caactaaagc
    38821 atgcgaatta cggcgcgggg aattagcgat ggagttttag gataattgga atgaatatca
    38881 aatataagtt tcaacttata tttaaataac aataaaaatc ataagcttaa aataaaaaac
    38941 aaaagtaatt aatctgcgta ttctccgaaa taaacaacga ttaaaaatct attttaggat
    39001 taacctcacg gtatgatttc cgttaaatca cagacaaggg gatttatctg ataaaacaat
    39061 gccgacttat tgtcggcatt gtttttaagg ttatttaact cctcatacta tgcattaatg
    39121 tcgatattgg catcaggctc cggttgccgg atgcggcgta aacgccttat ccggcctaca
    39181 tatcggcatc aggcacgcat gatgattaac cgcgccacgc tttatagcgg ttaatcagac
    39241 cattggtcga gctatcgtgg ctgctgattt ctttatcatc tttcagctct ggcagaatac
    39301 ggtttgccag ttgtttaccc agctccacgc cccactggtc gaaggtgaag atgttcagga
    39361 tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgcaccc aggctgaacg
    39421 gagtgatttc gcgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg
    39481 gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca
    39541 cttcgcggga tttaccaaac gccagcgctt cggtctgggc gaagaagtta gacagcagtt
    39601 tctggtggtg atcagagagc gggttatggg tgatagccgg ggcgatgaaa tcgcacggta
    39661 ccattttggt tccctggtgg atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt
    39721 caccccagat aatcgggcca gtctggtaat ccacaacgtt accgttacgg tcaacatact
    39781 taccgttgga ctccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt
    39841 catacggcag aatcgcttca gtttccgcac caaagaaatt gttgtaccag atgccaatca
    39901 gcgccagcag gacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccattg
    39961 cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca
    40021 ggccaatcgc tgaccacaaa gagtaacggc cgccgaccca gtcccagaac tcgaacatgt
    40081 tggcagtatc aataccaaac tcgccaacgg ctttggcatt ggttgagagc gccgcaaaat
    40141 gtttcgcaac gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg
    40201 cgttggtcat ggtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt
    40261 ccgggtttac ttttttcagc acttccgaga tgtgagtccc atcgacgtta gaaacaaagt
    40321 gcatgttcag gtggtttttg tacggacgca gtgcttcggt caccatgtac gggccgaggt
    40381 cagaaccgcc gatcccgatg ttcactacgt cagtgattgc tttgccggta taacccttcc
    40441 actcaccgga gataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt
    40501 ctggcataac atctttgcca tcaaccagaa tcggggtatt gctacgatta cgcagcgcta
    40561 cgtgcagcac ggcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa
    40621 tcgcgcccgc cagatcgcac tctttcgcca gatcctgtaa tttcgccagc gtctcttcag
    40681 tgatgcggtt tttggagtaa tccaccagca tctgatcgtc gaaggttgcg gagaacttag
    40741 aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga
    40801 agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca
    40861 atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa
    40921 tgtgatggtt ttagtgccgt tagtgtaatg ttgagtgtaa acccttagcg cagtgaagca
    40981 tttattagct gaactactga ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt
    41041 gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga
    41101 agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg
    41161 ggtgcatcgc cgaggtgatt gaacggctgg ccacgttcat catcggctac aggggctgaa
    41221 tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt
    41281 ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg
    41341 tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc
    41401 aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg
    41461 ctttctgatg ccaacgtgcg tttagtagtc ctctcggctt ctgctggtat cactaatctg
    41521 ctggtcgctt tagcggaagg gctggaacct ggcgagcgat tcgaaaaact cgacgctatt
    41581 cgcaacatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag
    41641 attgaacgtc tgctggagaa cattactgtt ctggcagaag cggcggcgct ggcaacgtct
    41701 ccggcgctga cagatgagct ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc
    41761 gaaatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt
    41821 accaacgacc gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg
    41881 ctgcagctgc tcccacgcct caatgaaggc ttagtgatca cccagggatt tatcggtagc
    41941 gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac ggcagccttg
    42001 ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac
    42061 accaccgatc cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa
    42121 gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca
    42181 gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg
    42241 ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat
    42301 cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggctt cctcgcggaa
    42361 gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac gtcagaagtg
    42421 agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg
    42481 caatctctgc tgatggagct ttc
//
LOCUS       DAJTYL010000021        41806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYL010000021 DAJTYL010000000
VERSION     DAJTYL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.021"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..181
                     /locus_tag="OO572_001119"
     CDS             <1..181
                     /locus_tag="OO572_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCR6660198.1"
                     /translation="VYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIE
                     AGEFKATSTFQVTYP"
     gene            294..1088
                     /gene="panB"
                     /locus_tag="OO572_001120"
     CDS             294..1088
                     /gene="panB"
                     /locus_tag="OO572_001120"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6660199.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1100..1951
                     /gene="panC"
                     /locus_tag="OO572_001121"
     CDS             1100..1951
                     /gene="panC"
                     /locus_tag="OO572_001121"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6660200.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2025..2927)
                     /gene="rpnC"
                     /locus_tag="OO572_001122"
     CDS             complement(2025..2927)
                     /gene="rpnC"
                     /locus_tag="OO572_001122"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6660201.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3201..3581
                     /gene="panD"
                     /locus_tag="OO572_001123"
     CDS             3201..3581
                     /gene="panD"
                     /locus_tag="OO572_001123"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6660202.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3585..4814)
                     /locus_tag="OO572_001124"
     CDS             complement(3585..4814)
                     /locus_tag="OO572_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6660203.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4878..5318)
                     /locus_tag="OO572_001125"
     CDS             complement(4878..5318)
                     /locus_tag="OO572_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6660204.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5423..6193)
                     /locus_tag="OO572_001126"
     CDS             complement(5423..6193)
                     /locus_tag="OO572_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6660205.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6190..7116)
                     /locus_tag="OO572_001127"
     CDS             complement(6190..7116)
                     /locus_tag="OO572_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6660206.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7225..7887
                     /gene="can"
                     /locus_tag="OO572_001128"
     CDS             7225..7887
                     /gene="can"
                     /locus_tag="OO572_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6660207.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7928..8464)
                     /gene="hpt"
                     /locus_tag="OO572_001129"
     CDS             complement(7928..8464)
                     /gene="hpt"
                     /locus_tag="OO572_001129"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6660208.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8670..11060
                     /gene="gcd"
                     /locus_tag="OO572_001130"
     CDS             8670..11060
                     /gene="gcd"
                     /locus_tag="OO572_001130"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6660209.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11107..12657)
                     /gene="cueO"
                     /locus_tag="OO572_001131"
     CDS             complement(11107..12657)
                     /gene="cueO"
                     /locus_tag="OO572_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6660210.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12823..13170
                     /locus_tag="OO572_001132"
     CDS             12823..13170
                     /locus_tag="OO572_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6660211.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13276..14142
                     /gene="speE"
                     /locus_tag="OO572_001133"
     CDS             13276..14142
                     /gene="speE"
                     /locus_tag="OO572_001133"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6660212.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14158..14952
                     /gene="speD"
                     /locus_tag="OO572_001134"
     CDS             14158..14952
                     /gene="speD"
                     /locus_tag="OO572_001134"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6660213.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14990..15352)
                     /locus_tag="OO572_001135"
     CDS             complement(14990..15352)
                     /locus_tag="OO572_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6660214.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15527..18124)
                     /gene="acnB"
                     /locus_tag="OO572_001136"
     CDS             complement(15527..18124)
                     /gene="acnB"
                     /locus_tag="OO572_001136"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6660215.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18479..20332
                     /locus_tag="OO572_001137"
                     /pseudo
     CDS             18479..20332
                     /locus_tag="OO572_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20403..21827)
                     /gene="lpdA"
                     /locus_tag="OO572_001138"
     CDS             complement(20403..21827)
                     /gene="lpdA"
                     /locus_tag="OO572_001138"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6660216.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22152..24032)
                     /gene="aceF"
                     /locus_tag="OO572_001139"
     CDS             complement(22152..24032)
                     /gene="aceF"
                     /locus_tag="OO572_001139"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6660217.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24047..26710)
                     /gene="aceE"
                     /locus_tag="OO572_001140"
     CDS             complement(24047..26710)
                     /gene="aceE"
                     /locus_tag="OO572_001140"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6660218.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26871..27635)
                     /gene="pdhR"
                     /locus_tag="OO572_001141"
     CDS             complement(26871..27635)
                     /gene="pdhR"
                     /locus_tag="OO572_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6660219.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28176..29549
                     /gene="aroP"
                     /locus_tag="OO572_001142"
     CDS             28176..29549
                     /gene="aroP"
                     /locus_tag="OO572_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6660220.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29592..30446)
                     /gene="ampE"
                     /locus_tag="OO572_001143"
     CDS             complement(29592..30446)
                     /gene="ampE"
                     /locus_tag="OO572_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6660221.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30443..30994)
                     /gene="ampD"
                     /locus_tag="OO572_001144"
     CDS             complement(30443..30994)
                     /gene="ampD"
                     /locus_tag="OO572_001144"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6660222.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31082..31975
                     /gene="nadC"
                     /locus_tag="OO572_001145"
     CDS             31082..31975
                     /gene="nadC"
                     /locus_tag="OO572_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6660223.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32178..32618
                     /gene="ppdD"
                     /locus_tag="OO572_001146"
     CDS             32178..32618
                     /gene="ppdD"
                     /locus_tag="OO572_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6660224.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32628..33775
                     /gene="tadA"
                     /locus_tag="OO572_001147"
                     /pseudo
     CDS             32628..33775
                     /gene="tadA"
                     /locus_tag="OO572_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33765..34967
                     /gene="hofC"
                     /locus_tag="OO572_001148"
     CDS             33765..34967
                     /gene="hofC"
                     /locus_tag="OO572_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6660225.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35002..36045)
                     /gene="guaC"
                     /locus_tag="OO572_001149"
     CDS             complement(35002..36045)
                     /gene="guaC"
                     /locus_tag="OO572_001149"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6660226.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36207..36245)
                     /locus_tag="OO572_001150"
     CDS             complement(36207..36245)
                     /locus_tag="OO572_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660227.1"
                     /translation="MIIKTLKMSARK"
     gene            36270..36890
                     /gene="coaE"
                     /locus_tag="OO572_001151"
     CDS             36270..36890
                     /gene="coaE"
                     /locus_tag="OO572_001151"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6660228.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36890..37633
                     /gene="zapD"
                     /locus_tag="OO572_001152"
     CDS             36890..37633
                     /gene="zapD"
                     /locus_tag="OO572_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6660229.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37643..37840
                     /gene="yacG"
                     /locus_tag="OO572_001153"
     CDS             37643..37840
                     /gene="yacG"
                     /locus_tag="OO572_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6660230.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37940..38329)
                     /gene="mutT"
                     /locus_tag="OO572_001154"
     CDS             complement(37940..38329)
                     /gene="mutT"
                     /locus_tag="OO572_001154"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6660231.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38389..41094)
                     /gene="secA"
                     /locus_tag="OO572_001155"
     CDS             complement(38389..41094)
                     /gene="secA"
                     /locus_tag="OO572_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6660232.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41156..41668)
                     /gene="secM"
                     /locus_tag="OO572_001156"
     CDS             complement(41156..41668)
                     /gene="secM"
                     /locus_tag="OO572_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6660233.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
BASE COUNT        10032 a        11305 c        10373 g        10096 t
ORIGIN      
        1 tgtttataaa gattacgacc cgagaggcaa agacgataca acaggaggag tttacccgga
       61 tcaagataca ggtataacat accctctcca tttccaggcc acgctacaac aggatggaac
      121 tataccaata gaagctggtg agtttaaagc caccagtact ttccaggtaa cctaccctta
      181 ataagtccac cgcaccgcca tcatctggcg gtgcggtaat tgataaatca ttgcccccac
      241 caatgtgact gataaaatgc caggcaacac gacatcattt atcaggatac gttatgaaac
      301 cgaccaccat tgcttcactt cagaaatata agcaggataa aaagcgtttc gcgaccatca
      361 ccgcttacga ctacagcttc gcgaaactat ttgctgatga agggcttaac gtcatgctgg
      421 taggcgactc gctgggcatg accgttcagg gccacgattc cactctgcca gtaaccgttg
      481 ctgatatcgc ctaccacact gccgccgtac gtcgcggcgc accaaactgc ctgctattgg
      541 ctgacctgcc gtttatggcg tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg
      601 ttatgcgtgc cggtgccaat atggtcaaaa ttgagggcgg tgagtggctg gtcgaaaccg
      661 taaaaatgct gaccgaacgt gccgttcctg tatgtggtca cttaggttta accccacagt
      721 cagtgaatat tttcggtggc tacaaagtac aggggcgcgg caatgaagcg agcgatcgac
      781 tactcagcga tgcattagcc ttagaagccg cgggggcaca gctgctggtg ctggaatgcg
      841 tgccggttga gctggcaaaa cgtattaccg aagcactggc gatcccggtt attggtattg
      901 gtgcaggcaa cgtcactgac gggcaaatcc tcgtgatgca cgacgccttt ggtattaccg
      961 gcggtcacat tcctaaattc gctaaaaatt tcctcgccga aacgggcgac atccgcgcgg
     1021 ctgtgcggca gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt
     1081 tccattaagg agtcacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat
     1141 tcgccgcctg cgtatggaag gcaagcgcgt ggcgctggtg cctactatgg gtaatctgca
     1201 caatggccat atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag
     1261 tattttcgtt aacccgatgc agttcgaccg cccggaagat ctggcacgtt atccacggac
     1321 cttgcaggag gactgcgaga agctaaacaa acgtaaagtg gatttagttt tcgccccttc
     1381 ggtaaaagag atctacccga acggtactga aacccacact tacgttgacg ttcctggcct
     1441 ttcgaccatg ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgactattgt
     1501 cagcaagctg ttcaacctgg tccagccgga catcgcctgc ttcggtgaaa aagattttca
     1561 gcaactggcg ttgatccgca aaatggttgc cgatatgggc ttcgatattg agattgtcgg
     1621 tgtgccaatt atgcgcgcca aagacggtct ggcgctaagt tcccgtaacg gttatctgac
     1681 ggcggaacaa cgcaaaattg cgcctggtct gtacaaagtt ttaagttcga ttgctgacaa
     1741 attgcaggct ggggaacggg atctcgatga aattattacc attgcggggc aagaactgaa
     1801 tgaaaaaggc ttccgcgccg atgatattca gattcgcgat gccgacacat tgctggaagt
     1861 ttctgaaacc agcaaacggg cagtaattct ggtagccgcc tggcttggcg atgctcgcct
     1921 gatcgacaac aaaatggtcg agctggcgta atacttaact ggcgctacgg ctgatggcgc
     1981 cagttattaa tttaccccac gtcatctgtc ataacacagg taacttaaat aaggttaatt
     2041 accttatcaa tctcagcaag aggtaaattt gccatctctg caacgtcttc ccgagacatt
     2101 cctttactta gaagacgcag ggcgaattct tgcctttcct cctgtcttcc ctgctgaatc
     2161 cccttctcaa tccctttctc ttcaaaccac tgcgccagcg tcatcataga cttccctccc
     2221 gtttccctgt ctctcagcac accataaaac aaatccgctt gttcagtatg accgcgttgc
     2281 agcatatagt tttgcatggc aactaactga cttccgctag tgtacccttc gtcaatcagc
     2341 gtgaccagtt gctccagcaa taacattaag tcgcgctggc gaatatgttt ttgcagtagt
     2401 tcgagaatcg caatccgccg atgttgcatg atttcgtcat ccggtgtgat ggtgatatcc
     2461 accagcggga aaggactgtt atagacgcgt cgcgccagct ccggcgagta aaacatatca
     2521 aaccagcaca ttgagagcgg ataaggtgtg gcctcgccct gataaaacag tatcggcacc
     2581 accagcggca gcttatcgtg gccagcctcc agatgccggt gcatggcagc aatagaataa
     2641 cgcatcatgc gaaaggccat tttcttatcc ggcttgcttt ggtgttcaat cacaacatgc
     2701 agataaccgg gattgccctg catttgcatg gaatagagca cgtccgtgct gtgacctttc
     2761 aggcactctt caatgaaact ccccgactct aaatgaagcg tgttgagatc acaaagttcg
     2821 cgtaattcca ctggcaaatg tatctccaga aagtcgcgag ccgtctccgc atgcattaaa
     2881 aattgtttaa ataccgcgtc atgcggtgtg gtactcggtg catccatctt ccctgtcttc
     2941 cgtgtggcct tgcgtgaagg cagtatccag tcaaggcgtg ctatcgtcag ccacctcttt
     3001 attgtttacc gagcagcgtt cataaaaata tcaattcgtt acaggcgtta catggcacgc
     3061 ttcggcgcgg tacgcaaact gctgacgcaa atgccatcag atgagtagac actcatcaaa
     3121 aatcgggcaa tactgcgtga gaatttcctc aaagcaggca ccgcctgctt cgttaacgac
     3181 agggtagaaa ggtagaagtt atgattcgca cgatgctgca gggcaaactc caccgcgtga
     3241 aagtgactca tgcggacctg cactatgaag gttcttgcgc cattgaccag gattttcttg
     3301 acgcagccgg tattctcgaa aacgaagcca tcgatatctg gaatgtcacc aacggcaagc
     3361 gtttctccac ttatgccatc gcggcagaac gcggttcgag aattatttct gttaacggtg
     3421 cggcggccca ctgcgccagc gtcggcgata ttgtcatcat cgccagcttc gttaccatgc
     3481 cagatgaaga agctcgcacc tggcgaccca atgtcgccta ttttgaaggc gacaatgaaa
     3541 tgaaacgtac cgcgaaagcg attccggtac aggttgcttg attgttatcc ctgcggctgg
     3601 ttactcacca gccgcgacat cttctccagc gaatccgttc ttaagatata aagtcgtttt
     3661 agtaacaacg gattatcccc cggttttact ttgcctttca tggttgtcac cgccaggtga
     3721 aatccggcat cgtttgctgc cttcacggct ttgtcattaa agccgccaaa cggatacgaa
     3781 agataccaga catgtggatt aaattgcgcc agagcgcggc gtgaacgtgc aaaatcaaac
     3841 agaatattgt gctcactacg gctcagtaat atgggtcggc gataaccatc tacccgatgc
     3901 aaaaaatggg tatgtgactg gaaatcaaaa acatcgcgaa tttcgttaag ctcagaaacg
     3961 ctcataaatt gcagcgattt tgggttccac ttctgcgggt gacgtttgat gcgtgaggta
     4021 acaataaaca ccgtcgcctt catgccatat tgtttcaaca caggatacgc atagcggctc
     4081 accgacttga ggccatcatc aaaggtaatc accaccgctc gcgcagggag attgatcgta
     4141 ttcttcacgt aaccttccag ctgagccatg ctcagtgtcg cgtatcccct gtcgcgcagc
     4201 caggccattt ggttattgaa agcgcgtacc gaggtggtcg tcgaagtatg gcgaaaacgg
     4261 gtgttttctt cgtcgcgcag aatatggtga taagttagca ccggcaggcc attatcgggt
     4321 tgggcatcca gcgcgctgat ataggccagt cgatcgccaa tacggatctg ataccaggtt
     4381 tgatttaacc tgtctttcag tttatgcaaa atcgggtagc gcaaattgtc cgccagtacc
     4441 ccaaatggcg cactgcccac actcggcgcg ttgtagaccg gcgtatcttt ccaggtaatt
     4501 aagttctgat tactcagcgg cttgttgagg tcacccaaac cgtcttcaac tttttgtcgt
     4561 ccctgaactg gctcgagatg ccctttatcg ataaaccctt taccaaagcc aaaattaaat
     4621 gcgtaataac ttgcggcagt gggctccacg gcaatgattt gtccggcccg aatattcccc
     4681 acggtcacca tcttgtcact aatttgcgcc cagaccgcag cattttcgat ggtttgcata
     4741 taacgggcag gtaacgcggc actgacactt gcggtaaaca gcatcagcag gagaataaca
     4801 gcttgtttat acatgaaaaa ataaccgagg gtgaaacaac gcttttattg tagcaaaagc
     4861 gccatcagaa tcgcgggtta atactcatac aaattatgtt tgaggacgaa aggcggattt
     4921 ttttgttgtt ggtgccaaag actactcacc tccggcccac ccagttcgac aatacactca
     4981 cgaaactctg aactggtcat ggtttcacgg caagccatca tctgttcaat taacggtaac
     5041 gtgacaccag aaatcacttc gcaacgggag tgtttgtggc ttaataatga agccacgcga
     5101 tacggtggcg ctccggctat atcggttaag aagatgacac cctcaccaga gtccgcttca
     5161 tgcagagcat cgcacatcat gcggctgagc atattagagc ttaatccgcg ccagaaattc
     5221 acggcccggc actgaagaag tgccccatgt tttttctcca gcgcatccag tatctcttgc
     5281 gcccggtcat cgtgacaggt aattacccaa cctaacatcg ccttcctcct tagccgaaaa
     5341 tcagcctacc ctgaccattg ttgataaagg gtgatagccg tcaaaactcc tctcccccga
     5401 atccagagga gaggaaatag ccttagctac gcaaaccacg tccacgttgg atcagcgacc
     5461 aacagatcaa ataaaacgcc acaataaaga ccaccagtac gccaaaggta gtgaccagcg
     5521 gcacatcatt tataccgagg aagccgtagc ggaaaccact gatcatataa acgattgggt
     5581 tcaggtgcga cagcccttgc cagaacggcg gcaacaaagt cagtgagtaa aagaccccgc
     5641 ccaaatacgt gagtggcgtt aacacaaagg ttggcaccag gctgatgtca tcgaacgttt
     5701 tggcaaacac gccgttcagc aaacccgcaa gggagaatag caccgccgtg agcaccaggg
     5761 ttaaggcaac gaatacccac gaatgcacct gaaacggcac aaaaaacagt gaaattgccg
     5821 tcacaagaat gccaacaaac agaccacgcg ccacgccacc gccgacatat ccggcaataa
     5881 tgacgtgagt cggaaccggc gctaccagca gctcttcaat attacgctgg aacttggcac
     5941 caaaaaatga tgacgcgacg ttagcgtagg cgttggtgat caccgacatc atgatcagcc
     6001 ccggtacgat gaactgcata tagctgaagc catgcatatc gccaatacgc gaacctatca
     6061 ggttaccgaa gataataaag taaagggtca tggtgatgac tggcggcacc agcgtctgca
     6121 cccagatacg cataaagcga tggatctctt tcgcccagat gctttttagc gccacccagt
     6181 aaagatgcat catgcgcgat ctccttgttt ttcattaacc agtgaaacaa acagctcttc
     6241 cagacggtta gctttgttac gcatacttaa tacctgaatg ccctgctcac ttaactgcgt
     6301 aaatacgctg ttgatcccct gctcacgtag cacttcaact tccagcgtcg cggtatcgac
     6361 cagtcgatac tgatagccat cgagcttcgg taacggactt ttcggtgcga gatcgagaat
     6421 aaaggtttcc gatttcagct tcgccagcag cgccttcatc gaggtatttt ccaccagctc
     6481 accatgttga ataatgccga tattgcggca cagcatttct gcttcttcca ggtagtgtgt
     6541 ggtaagaatg atggtggtgc ctttgtcgtt taaatccttc aaaaagcccc acattgagcg
     6601 gcgaagttca atatccacgc ctgcggtcgg ttcgtcgaga atcagtagtt taggttcatg
     6661 cattaacgca cgggcaatca ttaaacggcg cttcatcccg ccagataaca tacgcgcacg
     6721 ttcgttgcgt tttccccata aatcgagctg cttaagatat ttttcgctgc ggatgtacgc
     6781 ctctttgcgc tccacaccgt agtaccctgc ctgattcacc acaatttgct gcacggtttc
     6841 aaacgggttg aagttaaatt cctgcggcac cagtcccaac tgacgtttag cgttcacgac
     6901 atctttctcg agatcgtaac caaatacgct gacccgcccg gaggttttat ttaccagaga
     6961 gctgataata ccgatagtgg tcgatttccc ggccccgttc ggcccgagaa gcgcataaaa
     7021 atcacccgct tcgacctgca aatctattcc acgaagcgcc tgaacgccgc ctggatagtt
     7081 ttttttaagc tgttgaagtt ccagtgcaat ggtcataaat ttttacttac cttacgttct
     7141 tacactttat ctgtggttta aatcgtcccg gagttgccct atattagcca aacgtaatta
     7201 tttggttaca ggtcgttaac ctccatgaaa gacatagata cactcatcag caacaatgca
     7261 ctatggtcaa aaatgctggt ggaagaggat cccgggtttt ttgagaaact ggcacaagcg
     7321 caaaaaccgc gctttctatg gattggatgt tccgacagtc gcgttcctgc agaacgttta
     7381 accggtcttg agccgggcga actctttgtt caccgtaatg ttgctaacct agtcattcac
     7441 accgacctga actgcctttc cgtggttcag tatgcagtgg atgtactcga agttgaacac
     7501 attattatct gtggccacta cggttgcggc ggcgtacaag ccgccgttga aaacccggaa
     7561 ctggggctta tcaacaactg gctgctgcat atccgcgata tctggttcaa acatagctca
     7621 ttgctcggcg aaatgccgca agagcgccgt ctggatacct tgtgtgaact gaacgtcatg
     7681 gaacaggtgt ataacctggg ccactccacc attatgcaat cagcgtggaa acgcgggcag
     7741 aaagttacca ttcacggctg ggcctacggc attcacgacg gcttgctgcg tgatctggat
     7801 gttaccgcca ccaaccgcga aacccttgag caacgttacc gtcacgggat ttccaacctc
     7861 aagctgaaac acgccaacca caaataaaaa tgccatgccg gatgcaacac atccggcaac
     7921 ttcacactta ctcgtccagc agaatcactt tgccaatata cggcagatga cggtaacgct
     7981 gtgcgtaatc aatgccgtaa cccaccacaa actcatccgg gatcgagaaa ccgataaatt
     8041 caaccgggac gttcacttca cgacgggacg gtttatccag cagcgtacaa atcgccaacg
     8101 acttcggttc gcgcaggctt aagatctcac gcactttcga cagtgtattc cccgagtcga
     8161 tgatatcttc aacaatcagc acgtccttgc cacggatatc ttcatccaga tctttgagga
     8221 ttttcacatc acgggtggtg gacatgccgc taccgtagct ggaggcggtc ataaagtcga
     8281 cttcatgaga tacctgaact tcacggcaca ggtccgccat aaacataaat gagccacgca
     8341 gcagacccac cagcaccata tcgctgccgc tgtctttgta acgctcagta atctgacgac
     8401 ccagttcggc gatacgcgct ttaatctccg cttcggggat cattacttct acagtatgtt
     8461 tcatatctct aaccatatga tttaaaaaca aatcacttcg cacgggcagg ttttggcacc
     8521 gcaacaaagc gcaagacgcg cagtatacca gtaaaactat tcattcgcgg aatctgttaa
     8581 taaagctcga attgtgatga cggtcacaca tgttaaaacc tataattaat tgctactaaa
     8641 atattaatga attgaaatgg tgtctcttta tggcaattaa caatacagcc tcgcgacgat
     8701 tactcgtcac gctaacagcc ctttttgcag cgctttgcgg gctgtatcta ctcattggcg
     8761 gaggctggct ggtcgcgatt ggcggctcct ggtactaccc tatcgctggc cttgtgatgc
     8821 tcggcgtcgc ctggatgctg tggcgcagta aacgcgccgc gctttggcta tacgcagccc
     8881 tgctgctcgg caccatgatt tggggcgtct gggaagttgg tttcgacttc tgggcgctga
     8941 ctccgcgcag cgatattctg gtcttcttcg gcatctggct gatcctgccg tttgtctggc
     9001 gtcgcctggt cattcctgcc agcggcgcag ttgccgcact ggtggtcgca ctgctgatta
     9061 gcggtggtat cctgacctgg gccggattta acgatccgca ggagatcaac ggcaccttaa
     9121 gcgccgatgc cacacctgcc gaagctatct cccccgttgc cgatcaggac tggcctgcgt
     9181 atggccgcaa tcaggaaggt caacgctttt caccactgaa gcaaattcac gccgataacg
     9241 tccacaagct gaaagaagcc tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc
     9301 cgggtgaaat caccaatgaa gtgacgccga ttaaagtggg cgataccctt tacctgtgta
     9361 ccgctcacca gcgcctgttt gcgcttgatg ccgccagcgg caaagagaaa tggcattacg
     9421 atcctgagct gaaaaccaac gagtctttcc agcacgtaac ctgccgtggc gtctcttatc
     9481 atgaagccaa agcagaaacg gcttcgccgg aagtgatggc ggattgcccg cgtcgtatca
     9541 ttcttccagt gaatgatggt cgcctgattg cgattaacgc tgaaaacggc aaactgtgcg
     9601 aaaccttcgc caataaaggc gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg
     9661 gtctgtatga gccgacttct ccgccaatta tcaccgataa aaccatcgtg atggccggtt
     9721 cagtcaccga taacttctca acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca
     9781 acaccgggga gctgctgtgg gcttttgatc cgggcgcgaa agatccgaac gcaatcccgt
     9841 cagacgaaca cacctttacc tttaactcac caaactcgtg ggcaccagcg gcctatgacg
     9901 cgaagctgga tctggtctat ctgccgatgg gcgtgaccac gccggatatc tggggcggta
     9961 accgcacacc ggaacaggaa cgttatgcca gctcgattct ggcgctgaat gccactaccg
    10021 ggaaactggc gtggagctac cagaccgttc accacgacct gtgggacatg gatcttccgg
    10081 cacagccgac gctggcggac atcaccgtta atggtcagaa agtgccgatt atttacgctc
    10141 cggcgaaaac cggtaacatt tttgtgctcg atcgtcgtaa tggcgaactg gtggttccgg
    10201 ccccggaaaa accggttccc caaggtgctg ccaaaggcga ttacgttacc cctactcaac
    10261 cattctcgga actgagcttc cgtccgacga aagatttgag cggtgcggat atgtggggag
    10321 ccaccatgtt tgaccaactg gtgtgccgcg tgatgttcca ccagatgcgc tatgaaggca
    10381 ttttcacgcc gccatctgaa cagggtacgc tggtcttccc gggtaacctg gggatgttcg
    10441 aatggggcgg gatttccgtt gatccaaatc gtgaagtggc aattgccaac ccaatggcac
    10501 tgccgtttgt ttcgaaactg ctcccgcgtg gtcctggcaa cccgatggag cagccgaaag
    10561 atgccaaagg cacgggtacg gaatccggca ttcagccaca gtacggtgta ccgtatggtg
    10621 tcacgctcaa cccgttcctc tcaccatttg gtctgccatg taaacagcca gcatggggtt
    10681 atatctcggc gctggatctg aaaactaatg aagtggtgtg gaagaaacgt attggtacgc
    10741 cgcaggacag tatgccgttc ccgatgccgg ttccggtgcc gttcaatatg ggtatgccga
    10801 tgctgggcgg gccaatctcc acggcgggta acgtgctgtt tatcgccgct acggcagata
    10861 actacctgcg cgcttacaac atgagcaacg gtgaaaaact gtggcagggt cgtttaccgg
    10921 cgggtggtca ggcaacaccg atgacctatg aagtgaatgg caagcagtac gtggtgatct
    10981 ccgcaggcgg tcacggttca tttggtacga agatgggcga ctatattgtg gcttatgccc
    11041 tgccggatga tgtgaaataa ccataatgcc cggagagatc cgggcatatt tccgaatacg
    11101 gtctttttat accgtaaacc ctaacatcat ccccgtatct tcatgctcca gcagatggca
    11161 gtgcgccata taagcatgtt ctttcggtgc gttgtgatta aacttcacca gcacttcact
    11221 gacattgcct tctaccttaa cggtatcttt ccagcccgcg cgatgagccg ctggcggttt
    11281 gccattttct gacaagatac ggaactgcgt gccgtgaata tggaacggat gcagcatcat
    11341 gtcgcccacg ccagagataa cccaacgttc gtactgccct ttcgccgccg caaacatcgg
    11401 cttattcata tcaaacgcct gaccgttgat tttattggca tggtggaaat cgaacttccc
    11461 gccgtggttc atatgattca tattgccgtg ccccatatgg cccatcatct ggctgtgatc
    11521 cattccggcc atcgcctgat cgccatattt ctccattagc atctgcatcc ccatcatatc
    11581 gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa
    11641 cgcaggcagg ctacttaatg tgtcaggcaa agcaccggag gcactaatag caatcggctg
    11701 aatccgcatt accggatgcg gcttgtcaaa cggtgcaatc accatcccca tctggctgac
    11761 cggcagcgtc accaggtcaa agggtttgtt gtcgttaacc tccaccagca cttcaaaacg
    11821 ctcgcccatc agcaccggca gctcgctcac cttcaccggt tcaggtagca gaccaccgtc
    11881 gctggcaatc acatacagcg ggcgattgtc gctggtggcg aaattgagcg agcgggcatt
    11941 acagccattg agcaaacgca ggcgcagcca accacgcggg gcagcgtgtt gtgggtagat
    12001 tgcaccgttg gtcagcagcg tatcgccaaa ccagcccacg gcggcggtca tcacatccag
    12061 ttgataatca atctgcccgt cggcgctaaa tttcttatcc tgaacgatca ccggaacatc
    12121 atcgataccc cactgttttg gcagcattaa tttcaggatc tcgtcatctt caatcaccac
    12181 cagaccagcc agccccatcg ccacctgtcg cccggtcttg ccatgttgat gtggatggaa
    12241 ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc gagcgcttgc cacctggcgg
    12301 aataattccc tgtgggccgc cgtcgacgtc acccggtact tccagcccgt gccagtgcaa
    12361 cgtcgtctct tccgccagtt ggttgtagat atcaaccgtt accgctttgc cgcgctgtaa
    12421 tttcaccgcc ggccccagca gattgccgtt atagccccag gtagttgcag ttttctcgcc
    12481 aaaggtggac tgacctgcgc caatagttaa ctgaatgcga ttacgggcat cggtcgtgag
    12541 caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag actgcacggc tccacagtgg
    12601 caaggctgaa gccacaccca gtgcgacgga atatttcaag aaatcacgac gttgcatagt
    12661 tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt
    12721 acgggaaggt caagccgcag acacaattta atcgacaata atggtcgtcg cctcgctcgc
    12781 agtgtgctaa cgtttatctt ctttaagccc ctggtagaag caatgaagac gtttttcaga
    12841 acagtgttat tcggcagcct gatcgccgtt tgcgcaaaca gttacgcgct cagcgagtct
    12901 gaagccgaag atatggccga tttaacggca gtttttgtct ttctgaagaa cgattgtggt
    12961 taccagaact tatctaacgg gcaaattcgt cgcgcactgg tctttttcgc ccagcaaaac
    13021 cagtgggatc tcagtaatta cgacaccttc gacatgaaag ctctcggtga agacagctac
    13081 cgcgatctca gcggcattgg cattcccgtc gctaaaaaat gcaaagccct ggcccgcgat
    13141 tccttaagcc tgcttgccta cgtcaaataa tccctgatac ctttttgtag aaatattgac
    13201 cgtgcatccg cggtcaatgt tagctattat gttgcgccct ttttttacgg gtgttaacaa
    13261 aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt
    13321 gggcagtact ttgcggtaga taacgttctg tatcatgaaa agaccgatca ccaggatctg
    13381 atcatttttg agaacgctgc atttggtcgc gtaatggcgc tggatggcgt agtacaaacc
    13441 accgagcgcg acgagtttat ctatcatgag atgatgaccc atgttccgct actggcccac
    13501 ggtcacgcga aacatgtgct gattatcggc ggcggcgacg gtgccatgct gcgtgaagta
    13561 acccgacata aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg tgtcgtgtcg
    13621 ttctgccgtc agtatctgcc caaccataac gccggtagct acgacgatcc gcgctttcaa
    13681 ctagtgatcg acgatggcgt caatttcgtt aatcaaacca gccagacctt tgatgtcatt
    13741 atctccgact gcaccgatcc tatcggtccc ggcgaaagcc tgttcacctc agcattttat
    13801 gaaggctgca aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa cggcgtctgc
    13861 tttttacagc aggaagaagc catcgacagc catcgcaaac tcagccatta cttcagcgac
    13921 gttggctttt atcaggcggc gatcccgacc tattacggcg gtatcatgac ttttgcatgg
    13981 gcgacagata acgacgcctt acgccatctc tcaaccgaaa ttattcaggc gcgttttctc
    14041 gcttctggcc tgaaatgccg ttattacaat ccggcaatcc atacggcagc ttttgcctta
    14101 cctcagtatc tgcaagacgc actggcttca cagccgtcct aaggagaaga taagaaattg
    14161 aaaaaactga aactgcatgg ctttaataat ctgaccaaaa gtctgagttt ttgtatttac
    14221 gatatctgct acgccaaaac tgccgaagag cgcgacggtt atattgctta tatcgatgaa
    14281 ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat tatcggggcc
    14341 aatattctta acatcgcccg ccaggattac gaaccacagg gtgccagcgt cactattctg
    14401 gtgagtgaag aaccggttga cccgaaactc atcgacaaaa cagaacaccc cggcccactg
    14461 ccagaaacgg tggttgctca tctcgataaa agtcatattt gcgtacatac ctacccggaa
    14521 agtcatcctg aaggcggttt atgtaccttc cgcgccgata ttgaagtctc tacctgcggc
    14581 gtgatttctc cgctgaaggc gctgaattac ctgatccacc agcttgagtc cgatatcgta
    14641 accattgatt atcgcgtgcg cggttttact cgcgacatta acggtatgaa gcactttatc
    14701 gaccatgaga ttaattcgat tcagaacttt atgtctgacg atatgaaggc gctgtatgac
    14761 atggtggatg taaacgtcta tcaggaaaat atcttccata ccaagatgtt gcttaaagag
    14821 ttcgacctta agcactacat gttccacacc aaaccggaag atttaaccga cagcgagcgc
    14881 caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg
    14941 ccagctgttt aacggctctg gcggagctcc caggctccgc cagatttatt tacttctgct
    15001 gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc ctcaacgccg catagcgaca
    15061 ggctttcttc gtcgtagtag ttcatcccct cttccatttc atcgccagcg aattccagtt
    15121 gattggcgcg aaccatcacc tcttcagcgt ccatccacag ggtgtattca tgccctgccc
    15181 gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc ttgttccact tcatcaagca
    15241 aggccaggtt ttctttcacc tcttcattaa accagtgccc gaccacttca tgccccatgg
    15301 acatacgcac ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc atagtgtgtt
    15361 cctctgctcc cggcaatgcg ccgaaatcgc atcgcgccat tgatgtaatt atcgcagcct
    15421 taccggagaa aaacagcgga acggcgatag tttagaaata aaaaatgccc gggaatctca
    15481 ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct tacttattac accgcagtct
    15541 ggaaaatcac cccatcggct ttttcggtgt actgagaaag ctggttgaag ttcagataac
    15601 gataagtatc aacggcagtt ttatctactt gcgcaacgta ggtctggtac tcttccggcg
    15661 tcggcagttt gccaatcagc gccgcaacag ccgccagttc tgcagaagcc aggaagacat
    15721 tcgcgccagt acccaaacgg ttcgggaagt tacgggtaga ggtggaaacc accgtcgcac
    15781 cgtctgctac acgcgcctgg ttacccatac acagggaaca gcccgggatc tcgatacgag
    15841 ccccgctctt accgaagacg ctgtagtagc cttcttccgt cagttgcgcg gcgtccatac
    15901 gggttggcgg tgccacccac aggcgggtcg gcaactgacc tttgtgcgca tccagcagtt
    15961 taccagcggc acggaagtga ccgatgttag tcatgcaaga accgataaat acttcgtcga
    16021 tcttctcgcc ctgtaccgca gacagcggac gtgcgtcatc cggatcgttc ggagcacaca
    16081 ggattggctc tttaatctcc gccagatcga tgtcgatcac tgccgcgtat tccgcatctg
    16141 catcggcttc cagcagctca ggattcgcca gccatttttc catgccctga atacggcgtt
    16201 ccagggtacg acgatcgccg taaccttccg cgatcatcca cttcagcagg acgatgttgg
    16261 agctcaggta ttcgatgatc ggttctttgt tcagcttgat agtacaacca gcggcagaac
    16321 gctcggcgga cgcatcggtt agctcaaagg cctgctcaac tttcagatcc ggcagacctt
    16381 caatttccag aatgcgacca gagaagatgt ttttcttgcc tttcttctca acggtcagca
    16441 gaccttgttt gatcgcatac agcggaatgg cgtgtaccag atcgcgcagg gtgatgcccg
    16501 gctgcatttt gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc
    16561 cagttgcggc agcaaacgcc accagaccag aacccgccgg gaaagagata ccgatcggga
    16621 aacgggtatg ggagtcacca ccggtaccga cggtatccgg cagcagcata cggttcagcc
    16681 aggagtgaat gacgccgtca cccggacgca gcgacacacc gccacggttc ataatgaagt
    16741 ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg atacgccgcg gtgtggcaga
    16801 aagactgcat caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac
    16861 gggtcatcgg gccggtggtg tcctgagagc ctacagaagt cattttcggt tcgcagtacg
    16921 cgcccggacg aatgcctttc acgccacagg cacggcctac cattttctgc gccagcgaga
    16981 agccgcgatc gctctcagcg acatctttcg cctgacggaa cacatcactg tgcggcagac
    17041 caagtgcttc acgcgctttg gtggtcaggc cacgcccgat aatcagcgga atacggccgc
    17101 cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc
    17161 ctgtttcgtg gttacgcact tcacctttgt acgggtaaac gtcaatcacg tcgcccatgt
    17221 tcaggttaga gacgttgact tcgattggca gtgcacccgc gtcttccatc gtgttaaaga
    17281 agatgggtgc aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg
    17341 gaatatcatc gcccataaac cacagaacgg agttcgtggc ggatttacgc gatgaacccg
    17401 tacccacaac gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga gcttcaatct
    17461 gtttgatcgg gccaacaaca cctggctggt ctggctcaat accttcacgg gcgtttttca
    17521 gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca cgcatccggt gccggagaaa
    17581 ggtcatcggt gttggtttcg ccagtgactt taaagacggt aacggtcagt ttttcagcca
    17641 gcgccgggcg attcaggaac cattcggcat ccgcccagga ctgcattacc tgcttcgcat
    17701 attcgttgcc tgctttcgct ttctcttcta cgtcatagaa gttatcgaac atcagcagcg
    17761 tgtgagaaag tgctttggca gcaataggtg ccagtttggc atcatccagc gcgtcgatca
    17821 gcggatgaat gttgtaacca ccctgcatgg tgcccagcag ttcgatagct ttttccggag
    17881 tcagcagagg ggatttagct tcgcctttag cgatagcagc caggaagcct gcttttacat
    17941 aggcggcttc atcgacgcct gggggaacac ggttggttaa cagatctaac aggaattctt
    18001 cttcgcccgc gggcgggttt ttcagcagct ctacaagcgc ggccatttgg tttgcatcca
    18061 ggggtttggg cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta
    18121 gcacgacggt tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc
    18181 tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt
    18241 ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag
    18301 gatggtctag agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac
    18361 ctttctcaac gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat
    18421 tcacagcacc tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat
    18481 gaaaatgact ttgccgttta aaccccatgt gctggcgcta atttgcagtg ccgggctttg
    18541 tgctgcctct gccgggctat atataaaaag ccgcacagtg gaagcgcctg tagaaccgca
    18601 atcgacacaa ctggctgtgt ctgacgctgc cgctgttacg cttcctgcaa cggtttccgc
    18661 acctcccgta atacccgcag tcgtcaaatc cgcattcagc actgcacaaa tagatcaatg
    18721 ggtcgcaccc gtcgcgctgt atcccgacgc cctactttcg caggtgctga tggcatcaac
    18781 ctatccgaca aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg
    18841 cgatgctgct attcaggcgg tatctgacca gccgtgggac gccagcgtta aatcactggt
    18901 ggcctttcca caattgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg
    18961 cgatgctttt ctggcgcagc cgcaggacgt gatggactcg gtacagcgat tgcggcaact
    19021 ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag aaagttatta ccacaacgaa
    19081 gaaagctgta ccggtaaaac agacagtcac ggcacccgtc ataccatcca ataccgtttt
    19141 aactgccaac cccgtcatta cagagccggc aacaaccgtc atttccattg agcccgccaa
    19201 tcctgatgtg gtctatattc ccaactacaa cccaaccgtg gtttacggga actgggccaa
    19261 tactgcgtat ccgccggttt atctgccacc accagccgga gaaccgtttg ttgacagctt
    19321 tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg tacgcactat tcagcagcat
    19381 cgactgagat gacgacgatc atgaccatca tcatcatgac gatgatgatt atcatcacca
    19441 cgatggcggt catcgtgaca gtaatggctg gcaacacaac ggcgacaaca tcaatatcga
    19501 cgtcaacaat ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg
    19561 gcacaatcca aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt
    19621 tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag ctacctgctc caacacgcga
    19681 cagccagcgt caggcggcag caagtcagtt tcagcaacga acacacgccg cccccgtcat
    19741 tacacgagat acccaacgtc aggcagcggc acagcggttt aatgaagctg aacactatgg
    19801 gagctatgac gacttccgcg acttcagccg tcgccaacca ctgacccagc agcaaaagga
    19861 cgccgctcgt cagcgttatc agtcggcctc tcctgagcag cgccaggcag tccgcgagaa
    19921 aatgcagact aacccgcaga tccagcagcg aagggaggcg gcgcgccagc gtattcagtc
    19981 cgcctcgcct gagcagcgcc aggcggttcg cgagaaaatg cagactaacc cgcagaacca
    20041 gcagcgaaga gaggcagcgc gtgagcgtat tcagtctgca actcccgaac agcgccaggt
    20101 gtttaaggaa aaagtacagc agcgcccact gaaccaacag caacgtgata acgcccgcca
    20161 gcgtatacaa tcggcatcac ctgaacaacg tcaggttttt cgggagaaag ttcaggagag
    20221 ccgcccacaa cgtctaaacg acagtaacca tactgtcagg ctgaataacg agcaacggtc
    20281 agcagtacgc gaacgtctct ctgaacgtgg agcaagaaga ctggaaaggt aaattgcaga
    20341 cgtaaaaaaa gcggcgtggt tagccgcttt tttaattgcc ggatgttccg gcaaacgaaa
    20401 aattacttct tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc gaacacttct
    20461 gccgccaggc ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct
    20521 tcagcatcac aacccatttc gattgccagg ccgatttcac ccagcagctc gccgccgttg
    20581 gtaccaacaa tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc
    20641 ataccgtctg cgcagtcaga agcgatagca cgaccagaag cagcccacgg gaaggtggcg
    20701 gtttcatagc tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact
    20761 tctggttcgg tataggcgat ggacgggata actttcggat cgaagtagtg tttcttaccg
    20821 gcgataactt cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga
    20881 ccgacgatat cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg
    20941 cggatgaaac cacggtcgtc aacttccacg ccagctttgc ctgcgtcgag gtttttaccg
    21001 ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agctggtgct
    21061 tttttgcctt ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg
    21121 gtttccagca tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct
    21181 ttgtcagctg ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc
    21241 gcgtggtaaa cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt
    21301 tctggtactt ctttcagttc cagcgcgtca gtggagtccc agatacgcgg atcttcatgc
    21361 ggaataaacg gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg
    21421 atcacggttt tgccgttctc accttcaact tccagggtgt tagccccggt gaatttaccc
    21481 agaccgttga ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc
    21541 tgattgatca ctttctcttt ccaggtacga atcttgtcaa tatcggtttt cggttcgccg
    21601 aagacgatac cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc
    21661 agtgctttag aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt
    21721 tctacgatta cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg
    21781 gggcctgccc caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata
    21841 tatttatctc cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc
    21901 cctcacccca gtcacttact tatgtaagct cctggggatt ctggcgcttg ctaccttgct
    21961 gcacccagaa atccataggg tatgtgaagt aataccctaa ccaccaccgg gtcgttctat
    22021 ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc
    22081 tattcgctaa taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc
    22141 ggctctttta cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta
    22201 atgaaacggg caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc
    22261 agcatcagac gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa
    22321 acgccgagga tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg
    22381 ccgccgatgc tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg
    22441 tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg
    22501 ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg
    22561 ttgatgtatt tcttcagggt cagacgctga ccgtcttccg acagcgaact attgaagcga
    22621 ggcatctgct caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc
    22681 acatccagct tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg
    22741 gtgatatcgg ttttgtcgaa gtgggtaaca tgcgggatca tcacccagtt acggctcagg
    22801 ttagcaccag aaattttctg gatacggccc agttccactt cttcgatttc gccaaacttg
    22861 ctgaagtcca ccttcggcca cggcagcatg ccagggatac caccgccagt cgccgccgga
    22921 gctgcttctg cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata
    22981 cgacctttac ggccagtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga
    23041 cggcggatca gcggggtcgc gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct
    23101 tccgctttcg cagccggagc ttcagctttt gctgccggag ccggcgctgc cgcttcctgt
    23161 ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgaacca
    23221 gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc
    23281 ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc agcggcaact
    23341 ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga
    23401 acgttaactt ctttcacgcc agctgctggt gcaggggtcg ctgccggagt cgcttcctgt
    23461 ttagccgccg gagctgccgc gcctgcttca cccgcgactt cgaagaccat aatcagcgag
    23521 ccggtagaca ctttgtcacc cacgttcact ttgatctctt tcacggtacc tgcaaacgga
    23581 gccggaactt ccatagaagc cttgtcgcct tctacggtga tcagcgactg ttcagcttca
    23641 actttatcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc
    23701 ggaacgttaa cgtcttttgc cgccgcagcc gcgggtgctg ctgccggagc tgcttctttc
    23761 ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata
    23821 atcagtgcgc cggtctgggt tttatcgcca acagagactt tgatctcttt aacgataccc
    23881 gcctgcggag atggaacttc catagaggct ttgtcgcctt ctacggtgat cagcgactgt
    23941 tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc
    24001 ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg
    24061 ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat
    24121 cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa
    24181 cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca
    24241 gtacgcgata gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat
    24301 agtcggtaga tgccactgcc ggcgcgtcgt tcatcacctg agcgatatac ggaacgcgcg
    24361 gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagct
    24421 cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct
    24481 ctgccgcttc acggacgtga cgcaggatgg aaccggagcc cagcagctga actttacctt
    24541 tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt
    24601 ccggcattgc cggcatgtgg tagttttcgt tcagcgtagt gatgtagtag taaacgttct
    24661 cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag
    24721 cgtaagccgg gtcgtaagag atacagttcg ggatagtcag cgactgaatg tggctgtgac
    24781 catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta ccgccgatca
    24841 ggaagccacg cgcttgctgg tctccagccg cccagcacag atcgccgata cgctggaagc
    24901 cgaacatcga gtaatagatg tagaatggga tcatcggcag attgttcgtg ctgtaagagg
    24961 tcgccgctgc cagccaggaa caccctgcgc ccagctcgtt gatcccttcc tgcagaatct
    25021 gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct
    25081 gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg
    25141 cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt
    25201 tcagagcacg aacgaaagcg atagtggtag agagctcttt gctctgctct tccagcagcg
    25261 cgccgaagtc ttgcaggctc ggcagctcaa gcttctcggt gaagttcggc tgacggcttg
    25321 gcagataacc gtgcagtttc tgacgctgag cgtgcagata ggtatgctct tcagaacctt
    25381 ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac
    25441 ggtcgcggat gtgacgcacg ccgtccatgt tcattttctt aacctggtgc gcgatgtttt
    25501 taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attactgtcg
    25561 ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac
    25621 caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt
    25681 caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct
    25741 ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac
    25801 gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt
    25861 cgaagatgcc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac
    25921 gctgcaggtt acagttgata acgaaaacca ggttatccag tttttcacgg gtagcgatgg
    25981 tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg
    26041 tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt
    26101 aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca
    26161 tcagtttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat
    26221 ccagctgctc ctgagtcaga cgaccttcca ggaaagcacg ggcgtaaacg cccggggaga
    26281 tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt
    26341 ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca
    26401 gttcgaggtc ttttttcgac gcacgcagca ccgtcatgat ggcgttccag cggatagctg
    26461 aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga
    26521 tggtgttgat gtagttgctg atacctgtgc ctgcggctac gtttacaccg cctttgcggg
    26581 cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga
    26641 ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac
    26701 gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc
    26761 attgaactct cgccagaaag ctcaataaga caggttctac gtttagttac cgcgctttta
    26821 tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt
    26881 cgttgctcca gacgacgcag agaacgctca cggcggctct cttcacggct tctgtcgagc
    26941 aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta
    27001 ccgaccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg
    27061 cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat cggctccata
    27121 caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc aatctgatac
    27181 tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag ctctatggcg
    27241 tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa
    27301 gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatgg
    27361 tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa
    27421 gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca
    27481 cgcaaggagg gacgggagac gtcgaactgt tttgccagtt cgcgttccgg tgggagtttt
    27541 tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg
    27601 gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc
    27661 gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct
    27721 tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat
    27781 atataacgtt tcaaagttgt aactatgcac aaatgtagac tttacgtagg aaagggattt
    27841 tttaacgatt attaaactat aaaaatccga attgaaccga ttcacttacc aattttttga
    27901 tttttaagtc aataaaaacg aatttaaatt cactctgcat attgagaggg gttgaggctg
    27961 agctttacaa acggtttctt tttaagcaac tcatcttcaa ccatgcataa agcgggtgca
    28021 ttcgctgccg cataccatta ttcttgatct gacggaagtc tttttgtaac aattcaaacc
    28081 tccttgatgt aaacaaatca atacaacaaa cggaattgca aacttacaca cgcatcactg
    28141 cgtagatcaa aaaaacaatc accgcacgag gtttcatgat ggaaggtcaa cagcacggcg
    28201 agcagctaaa gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg ggtggcgcga
    28261 tagggacagg gctattccta ggtagcgcct ccgtaataca gtccgcaggg ccagggatta
    28321 tcctgggtta cgccattgct ggttttatcg cctttctgat catgcgtcag ctgggtgaaa
    28381 tggtggtcga agaacctgtc gcaggctcct ttagccactt tgcttataaa tactggggca
    28441 gttttgccgg tttcgcctct ggctggaact actgggtact gtacgtttta gttgccatgg
    28501 cagagctgac tgccgtgggt aaatacattc agttctggta tccggaaatc ccaacctggg
    28561 tttctgccgc cgtattcttt gtggtgatta acgccatcaa cctgaccaac gttaaagtgt
    28621 ttggcgagat ggagttctgg tttgccatta tcaaagttat tgcggtagta gcgatgatca
    28681 tcttcggcgg ctggctgctg ttcagtggca acggcggccc gcaggcgagc gttagcaacc
    28741 tgtgggatca aggtggtttc ctgccgcacg gcttcaccgg actggtgatg atgatggcga
    28801 ttatcatgtt ctcgttcggt ggtctggaac tggtggggat caccgcagca gaagctgata
    28861 acccggagca aagtatcccg aaagcaacta accaggttat ctaccgcatc ctgattttct
    28921 atattggttc gttagccgtt ctgctctcac tgatgccgtg gacccgcgtt accgccgata
    28981 ccagtccgtt tgtgctgatc ttccacgagt taggcgatac ctttgtggcg aatgcgctga
    29041 acatcgtggt actgactgcg gcgctctccg tgtacaacag ctgcgtatat tgcaacagcc
    29101 gtatgctgtt tggtctggca caacagggga acgcgccaaa agcgctggcg tctgtcgata
    29161 aacgcggcgt accggttaac accattctgg tgtctgcgct ggttacagca ttgtgcgtat
    29221 tgattaacta tcttgctccg gaatccgcat ttggcctgtt aatggcactg gtggtatccg
    29281 cactggtgat caactgggcg atgatcagtc tggcgcatat gaagttccgt cgcgccaagc
    29341 aggaacaagg cgtggtaact cgcttccctg ctctgcttta tccgctgggt aactggatct
    29401 gcctgctgtt tatggcggcg gtactggtga ttatgctgat gaccccagga atggcgattt
    29461 cggtatacct gatcccggta tggctgatcg tgttaggtat cggctatctg tttaaagaga
    29521 aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc cctcacccgt acagggtgag
    29581 ggcaataatc tctacaccaa cgccccgtaa atcgtcagta gcgcaatcac caccacgacc
    29641 acgaacgagg ttttcttcgc cattgaaacc gctgccttcg gcgtctccac cttatcgaca
    29701 tgcggttcac gcgccagaga gaactgcgcc agacgcgtta atacctgata ttgcgaagta
    29761 tggaaatcac ccagcgaagc aaaccaggcc ggtaacgctt tctcaccatg accgatcaag
    29821 gcatatacca cacccgcaag acgaaccggc acccaatcca gtacatgaag cactgcatca
    29881 atgccggact gtaaacgatg atgcggcgtc tggtatcgcg ccagccagta ttgccatgca
    29941 cgcaagaacg cataccccat cagcgtaacg ggtccccagg ttccccccac aatcagccag
    30001 aacagcggtg caagataaaa acgaaagtta atccacagca atgcattttg cagctcacgc
    30061 aaatactcac gttcgtcgca gcctgccggg acgccgtgaa tcatggtgag ttcgccagcc
    30121 atcgtggcac gggcatggct atcattacgt gaagcagctg tcagataagc atgataatga
    30181 agacgaactt tacctgcgcc aatacacagc aaaccaatca gcagccacac cagcagcgtg
    30241 ggaacgttga acaatactcc ctgcaatgcg cgtaacagta aaaaagtcac acccatcgca
    30301 ataatggtca tgcctaacgt gcgcccgaga gaaaaatgtt tcacccgccg aaagaacgct
    30361 tcaagacgat gatcaagctg ccagtgctcg cccaacttaa acaggcgctc gaaaattaac
    30421 accagtaagg ttgtaaatag cgtcatgttg tctccttgct gaccagcgca cgaaaccgtg
    30481 cccaatcaaa tgcaggaccg ggatcggttt tccgatccgg cgcaatatca caatggcccg
    30541 tcatgttatt agcgatatcg ggatagcgat ctatcagtgc ccgcgtaacc gccgcaagct
    30601 gttgatactg cgcatcggta tacgccagag tatcggtgcc ttcaagctca atcccaatag
    30661 aaaaatcatt gcagcgttca cgcccctgat actgagagac tcccgcatgc caggcacgtt
    30721 tatcgaaagg aacatactgg actatttcac catcacggcg aatcaaacag tgagcggaga
    30781 cgcgcaaatg ggcgatctca gcaaagaaag gatgtgcctg cggatcaata gttccagtga
    30841 ataatgcgtc gatccacgga ccgccaaact cgcctggcgg caggctaata ttgtgcacca
    30901 ccagcagggt gggtgtttcg tcatccaggc ggcaatcgta atgtggtgag ggaacgcggc
    30961 gcgcgccaac cagccacccc tgttctaaca acatgcagga tctccttata tgtggtgcta
    31021 atacccggtt cagagtagca tgtttctacc ttatgattcg ttagctatct ggagttttaa
    31081 catgccgcct cgccgctaca accctgacac ccgacgtgac gagctgctgg aacgcattaa
    31141 tctcgatatc cccggcgcgg tggcccaggc gctgcgggaa gatttaggcg gaacagtcga
    31201 tgccaacaat gacattacgg caaaactttt accggaaaat tctcgctctc atgccacggt
    31261 gatcacccgc gagaatggcg tattttgtgg caaacgctgg gttgaagagg tgtttattca
    31321 actggcaggc gacgatgtca ccataatctg gcatgtggat gacggcgatg tcatcaacgc
    31381 caatcaaccc ttgttcgaac ttgaaggccc atcccgcgtg ctgttaacgg gcgaacgcac
    31441 tgcgcttaat tttgtgcaaa ccctttcagg agttgccagt aaggtacgcc actatgtcga
    31501 attactggaa ggcaccaaca cgcagttgtt ggatacgcgc aaaaccttac ctggcctgcg
    31561 ttcagctctg aaatacgcgg tactttgcgg cggcggagcg aatcaccgtc tggggctttc
    31621 tgatgccttc ctgatcaaag aaaaccatat tattgtctcc ggctcagtgc gccaggcggt
    31681 cgaaaaagcg tcctggctgc atccggatgc gccagtagaa gtcgaagtag agaatctgga
    31741 agaacttgat gaggccctga aagccggagc cgatatcatc atgctggata acttcgaaac
    31801 agaacagatg cgcgaagccg tcaaacgcac caacggcaag gcgctactgg aagtgtctgg
    31861 caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg ggcgtggact ttatctccgt
    31921 cggtgcgcta actaaacacg tacaagcact cgacctttca atgcgttttc gctaattgct
    31981 gacagattta tccgctccat cgcgggcgga taatctcccc ttccccaact ttcttcgtaa
    32041 cgcctcgcaa atttcttgtc tttcgctgca acgaaataaa aaacctcgga atctgctttc
    32101 ccgtttcgtt ttttgcccct cgcctgtgct gatggcgttt gccaaagtag caccaaccaa
    32161 atcaaggagc gaaacagatg gacaagcaac gcggttttac acttatcgaa ctgatggtgg
    32221 ttattggcat cattgccatt ttaagctcca ttggtattcc cgcttatcaa aactacctgc
    32281 gcaaagccgc actcaccgac atgctacaaa cctttgtgcc ttaccgtacc gccgtagagt
    32341 tgtgcgcgct ggaacatggt ggattagata cctgcgacgg tggcagcaat ggcattccct
    32401 cgcctaccac cacccgctat gtttcagcca tgagtgtggc aaagggcgtg gtgtcgctga
    32461 ccgggcaaga aagtctcaat gggctaagcg tcgtcatgac gccaggttgg gataacgcaa
    32521 acggcgtcac cggctgggcg cgcaactgca atattcaaag tgacagcgca ttgcagcaag
    32581 cctgcgaaga tgtcttccgc tttgatgacg ccaactaagg agcggcaatg aatattccac
    32641 agctcacggc cctgtgcctg cgttatcagg gagtcttgct ggatgccagc gaagaagtgg
    32701 ttcatgttgc ggtggtcgat gccccctcac atgagttgct ggacgcattg catttcgcta
    32761 ccaccaaacg tattgagatc acctgctgga cgcgccaaca aatggaaggt cacgccagtc
    32821 gcacacaaca gacattaccc gtagctgttc aggagaagca tcagcccaaa gcagagttgc
    32881 tgactcgaac gttacaatct gcgctggaac aacgcgcgtc tgatattcat atcgaaccag
    32941 cggacaatgc ctaccgcatc cgcttgcgta tcgacggcgt attgcatcct ttaccggatg
    33001 tttcaccgga tgccggagtc gcattaacag ccagattaaa agtgctggga aacctggata
    33061 ttgcggaaca tcgcctgccg caggacgggc aattcactgt cgaactggca ggaaacaccg
    33121 tctcatttcg tattgcgacc ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt
    33181 tacagcaggt gagccaggca ctggatgtta acacgctggg aatgcagccg ttacaactgg
    33241 cggactttgc tcatgccttg caacaaccac agggactggt gctggtaact ggccctacag
    33301 gcagtggcaa aacggtcacc tggtgttgtc cacgctacac accaattcca ccactgaaac
    33361 gctggtacgt ttacagcaaa tgggggtcgc ccgctggatg ctatcatcgg cgcttacgct
    33421 ggtaatagcc cagcgtctgg tacgtaaact ttgcccacat tgtcgccgac agcaagggga
    33481 gcccatccat attccagaca ctgtatggcc gtcgccgctg ccccactggc aggcacccgg
    33541 ttgtgtacat tgctaccacg gtttttatgg tcgtaccgcc ttgtttgaag tcctgcccat
    33601 tacaccagcc atacgccagc ttatttccgc taatactgaa gttgaatcgc tggaaacgca
    33661 cgcccgacag gcgggtatgc gtacgctttt tgaaaacggc tgcctggccg tggagcaagg
    33721 cttaaccacc tttgaagagt taatccgcgt actggggatg ccgcatggca agtaagcaac
    33781 tctggcgctg gcatggcata accggcgacg gcaatgcgca agatgggatg ctatgggcag
    33841 agagccgtac tttactgctt atggcactac agcaacagat ggttacccca ctaagcctga
    33901 agcgaatcgc catcaattct gcgcagtggc gaggagataa aagcgcggaa gtcattcatc
    33961 aactggcgac gctacttaaa gccgggttaa cgctttctga agggctggct ctgctggcgg
    34021 aacagcatcc cagtaagcaa tggcaagcgt tgctgcaatc gctggcgcac gatctcgaac
    34081 agggcattgc tttttccaat gccttattac cctggtcaga ggcatttccg ccgctctatc
    34141 aggcgatgat ccgcacgggt gaactgaccg gtaagctgga tgaatgctgc tttgaactgg
    34201 cgcgtcagca aaaatcccag cgtcagttga ccgacaaagt gaaatcagcg ttgcgttatc
    34261 ccatcatcat tttagcgatg gcaatcatgg tggttgtggc aatgctgcat tttgttctgc
    34321 cggagtttgc cgctatctat aagaccttca acaccccact accggcacta acgcagggga
    34381 tcatgacgct ggcagacttt agtggcgaat ggggctggct gctggtgttg ttcggctttc
    34441 tgctggcgat agccaataag ttgctgatgc gccgcccgac ctggcttatc gcgcggcaga
    34501 aattgctgtt acgcatcccg attatgggtt cactgatgag gggacaaaaa ctcacgcaga
    34561 tctttacgat tctggcgctg acacaaagtg caggcattac ttttttacag ggcgtagaga
    34621 gcgtcagaga aacaatgcgc tgcccgtact gggtgcaact tctgacacaa atccagcacg
    34681 atatcagtaa cggtcatccc atctggctgg cgctaaaaaa tgccggtgag tttagcccgc
    34741 tctgtttgca attagtgaga acaggagagg catccggctc gctggacctc atgttagaca
    34801 acctcgccca tcatcatcgg gataacacaa tggcgctggc ggataacctc gcagccttac
    34861 tggaaccggc gttgctgatc ataacgggag gaattatcgg tacgctggtg gtggcaatgt
    34921 atctgccaat tttccattta ggcgatgcga tgagtgggat gggataacgc tggcgtgttg
    34981 ctccacgcca gcgttgggag attacaggtt gttgaagatg cggttttctt gttccagcac
    35041 acgaataaac gtggtgcgct tggtcagctc tttcaggcgt gaagccccaa cgtatgtaca
    35101 agctgaacgc aggccgccca aaatatcacg cgcggtattt tcaacctggc ctcgcagcgg
    35161 cagcttaacg gttttacctt ctgctgcgcg gtattccgca acgccgccaa cgtgacgttt
    35221 catcgcagac tcggagctca tgccgtagaa cagcataaat ttctcgccgt tctcctcaac
    35281 gatgcgaccg ccgctctctt cgtggcccgc cagcatgccg ccaagcatga cgaaatcggc
    35341 accgccgcca aaggctttcg ccacatcgcc cggcgtggta cagccaccgt cgctgacgat
    35401 cattccgccc agaccgtgcg ccgcatcggc acattcgatt accgcagaaa gctgcggata
    35461 accgacgcct gttttgacgc gagttgtaca aacagaacct gggccaatgc caactttaac
    35521 gatatcagca ccggagagga taagctcctc acacatttca ccagtcacta cgttgccagc
    35581 acaaatggtt ttggtcggcc acgcttcacg cgctttcgca acgaactgca cgaagtgttc
    35641 ggaataacca ttcgccacat caatacaaac gaagtttaat gccgggttca ggtcgagaat
    35701 ctgtttagtt ttttcgaaat ccgcatcaga cgtgccggta gaaaccatca catgtttcag
    35761 cacatcagcg gaagaattgt tgataaacgc ttgccactct tcgacagaaa agtgtttatg
    35821 cacagcagtc agaatatcaa aagaagccag cgcagaggcc atagaaaatg tgcctacggt
    35881 gtccatattt gcggcgataa tcggcacgcc ggaccagctc tgacctgaat gtttgaaggt
    35941 gaattgacgt tccagttcaa catcggaacg gcttttaaga gtggagcgtt tagggcggat
    36001 gagaacgtct ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg gattcctggg
    36061 gttaatggct ataagggtaa gagcgcaact ccagtgacgc tatcatacgc actaatcaat
    36121 gcggcgcaag actgcgaaat tgtctttttt tacgctacaa tcccataaat ttacctggta
    36181 aatagtgagc gaacgtttca tcaatttcac tttcgcgcgc tcatttttaa cgttttgata
    36241 atcatgatga attcccggga atataaatta tggggtatat agttgcctta acgggaggca
    36301 ttggcagtgg taagagtacc gttgccaatg cgtttgctga tctcggaatt aacgtcattg
    36361 atgccgatat tattgcgcgt caggtggttg aaccaggtgc acctgcgcta catgccattg
    36421 ctgatcactt tggcgaaaac atgattgctg ctgatggaac attgcagcgc cgggccttgc
    36481 gcgagcggat cttcgccaac ccggaagaga aaaactggct taacgacctg ctgcatccgc
    36541 tgattcaaca agagacgcaa caccagatcc agcaagcaac ttccccctat gtactgtggg
    36601 ttgtgccatt gctggtagaa aactcactgt ataaaaaagc gaatcgagtg ctggtggtgg
    36661 atgtcagccc agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta actcgcgaac
    36721 atgtcgaaca aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc gtggcagatg
    36781 acgtcattga taataacggc gcaccggatg ctatcgcatc ggatgttgcc cgcctgcacg
    36841 cacactattt gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa tgcagaccca
    36901 ggtccttttt gaacatccac taaatgaaaa aatgcgtaca tggctgcgca ttgagttttt
    36961 gattcagcaa ctcaccgtta atttacccat cgttgaccac gctggcgcgc tgcatttctt
    37021 ccgtaatgtc agtgaattac tggatgtttt cgagcgcggc gaagtccgca ctgagctgtt
    37081 gaaagaactt aaccggcagc aacgtaaact ccagacctgg attggcgtgc ctggcgtgga
    37141 ccagagccgt attgaagcat taattcagca gttaaaagcg gcggtgagcg tattaatttc
    37201 cgcgccgcgt atcgggcaat ttctgcgtga agatcgtttg attgctctgg tgcgtcagcg
    37261 actgagcatc ccaggcggct gttgcagctt tgatttacct acattgcaca tttggctgca
    37321 tctaccccag gcgcagcgcg acagccaggt agaaacctgg attgccagcc tgaacccgct
    37381 cacccaggca cttaccatgg tgctggattt aattcgccag tcggccccct tccgtaaaca
    37441 aaccagcctg aatggttttt atcaggataa cggtggcgat gccgacttgc tgcgcctgaa
    37501 tctgtcgctc gattcacagc tttatccgca aatttccgga cataagagcc gttttgccat
    37561 tcgttttatg ccgctggaca ctgaaaacgg acaggtaccg gaacgtctgg atttcgaact
    37621 ggcctgttgc taaggagtaa aaatgtcaga aactattacg gtgaattgcc caacctgcgg
    37681 gaaaacggtg gtgtggggtg aaatcagccc gtttcggcca ttttgctcca aacgttgtca
    37741 gctgatcgac ctcggagaat gggctgctga agaaaaacga atccccagca gtggcgatct
    37801 ttccgaaagc gatgactgga gcgaagagcc aaagcagtga catttgtctg atgcctcacg
    37861 taggcctgat aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg
    37921 cgtgaacgcc ttatccgacc tacagacgct taagcttcgc aattaccggt tcattggctg
    37981 gcggaaaatc atcggcatta agaccgacca gcgacatcca ctcaccgggt tgcccttctt
    38041 taccccacgg ccccccttcc caactttcga ccagccagaa ccacagagtt atatgcctgt
    38101 ccgggaattc atattccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgacct
    38161 cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc gcccatttca attttaccgc
    38221 cgggaaactc cagtttattc gccatgtgcg catctgctgc gcgacgtgtt ataaagattt
    38281 cattgttctc gttgcgaata atacctaccg caatttgcag ctttttcatt gtcttaaacc
    38341 tataaaaaag gcgcagaatc ctgcgccttt tacttcaact gttagctttt attgcaggcg
    38401 accatggcac tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac
    38461 tttgcgttca ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg
    38521 gctaagctgc tgcatttgtg ctaaacgctc ggcttccata cgacgctgtt gttccagctc
    38581 ctcaacctct tcaggcatac gtacctgaac tttgctcagc gtactgataa cttcatattt
    38641 caacgactcc agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg
    38701 atctttctgt gcatagccac gcaggtggat accctgacgc agatagtcca tcgctgccag
    38761 gtgctctttc cacagggagt caagcgtttg cagcatgacg cctttctcga agtgacgcat
    38821 catctcagca ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat
    38881 gcgctcacgc agcgtctctt catgcagttc tggttcttta tccagccact cggtaattgg
    38941 taaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc
    39001 ttccagcgac tgtggcggaa tgtaggcatc aatggtggct ttgaacacat cttcacgaat
    39061 gctgttgatg gtttcgctca catcgctgac atccagcagt tcgttacgct gggagtaaat
    39121 ggcgcgacgc tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa
    39181 gttacgactt tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc
    39241 aatggcttcg cctggcttca tacccagttt acgcatcatg ccggatactc ggtcggaagc
    39301 aaaaatacgc atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc
    39361 ctgacgacca gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt
    39421 accgatgata tgcaggccac ctgcttccag taccgcatcg tgacgtacct gccagtcggc
    39481 tttaattttt tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca
    39541 gctacctccg agcacaatat ctgtaccacg acccgccatg ttggtcgcga tagtcaccgc
    39601 agccggataa cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag
    39661 gacattgtgc ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat
    39721 ggagatagta cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat
    39781 gatcgcctga attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat
    39841 cattggacgg ttggtcggaa caacgacggt atccagtttg tagatggagc taaattcgaa
    39901 agcttcggta tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt
    39961 ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc
    40021 ttccacagcc tggtgcagac catcggacca gcgtcggccc tgcatggtac gaccggtgtg
    40081 ttcgtcaacg atgataactt caccatcttt aacgatgtag tcgacgtcac gggtaaacag
    40141 cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta
    40201 cagagactcc ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag
    40261 accacgttcg gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc
    40321 ctggaaggtt tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac
    40381 gcgtttatac atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc
    40441 ttcatcgatc aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac
    40501 acgttcttca gggctgaacg ccatgttgtc gcgcaggtag tcaaagccgt attcgttgtt
    40561 cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg
    40621 caggttgata ccaacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc
    40681 acgttgcgcc aggtagtcgt tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt
    40741 caggtaagca ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat
    40801 gcagcgttcg ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa
    40861 gacgcgctta cttgcctcac gcaccacggc gaaagcttcc gggatcagat tttccagcac
    40921 ttcgcctttt tccagacgcg cacgaaactc tgcggttttc cctttcagtt cttcatcgga
    40981 gagtttttcc atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg
    41041 cagggtgcga tcgttacgac taccgaaaac tttagttaac aatttgatta gcataataaa
    41101 atctcaaacg ccccgcgtta cggagttaat aaaatgaagt aaaggtttat tgttgttagg
    41161 tgaggcgttg agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga
    41221 attttgcttg tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc
    41281 cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag
    41341 gcaaagattc ttcagcaacg ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga
    41401 ttaccgtacg aatagcatgt tgatgccagt aatcaacgga atagttcgaa ttcgggcggc
    41461 gcgtgttcgc ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt
    41521 tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac
    41581 ctaaactcgc cgcgaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact
    41641 gtcgccagcg cgtcagtatt ccactcacgt tattgccatc ccgatcgaat tcaaacgtat
    41701 ttagcgcaca aatcttatca ttaaagcctg aagtccacag cacgaatgaa gataagttgt
    41761 gcgtaaaagt gcttaagaaa acagcgttcg caccgttttt tatagc
//
LOCUS       DAJTYL010000022        40236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYL010000022 DAJTYL010000000
VERSION     DAJTYL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.022"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(28..534)
                     /locus_tag="OO572_001157"
     CDS             complement(28..534)
                     /locus_tag="OO572_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6660234.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(580..1080)
                     /locus_tag="OO572_001158"
     CDS             complement(580..1080)
                     /locus_tag="OO572_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6660235.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1166..1345)
                     /locus_tag="OO572_001159"
     CDS             complement(1166..1345)
                     /locus_tag="OO572_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6660236.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1725..2531)
                     /gene="trpA"
                     /locus_tag="OO572_001160"
     CDS             complement(1725..2531)
                     /gene="trpA"
                     /locus_tag="OO572_001160"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6660237.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(2531..3724)
                     /gene="trpB"
                     /locus_tag="OO572_001161"
     CDS             complement(2531..3724)
                     /gene="trpB"
                     /locus_tag="OO572_001161"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6660238.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3736..5097)
                     /gene="trpCF"
                     /locus_tag="OO572_001162"
     CDS             complement(3736..5097)
                     /gene="trpCF"
                     /locus_tag="OO572_001162"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6660239.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5098..6693)
                     /gene="trpD"
                     /locus_tag="OO572_001163"
     CDS             complement(5098..6693)
                     /gene="trpD"
                     /locus_tag="OO572_001163"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6660240.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6693..8255)
                     /gene="trpE"
                     /locus_tag="OO572_001164"
     CDS             complement(6693..8255)
                     /gene="trpE"
                     /locus_tag="OO572_001164"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6660241.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8277..8370)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8347..8391)
                     /gene="trpL"
                     /locus_tag="OO572_001165"
     CDS             complement(8347..8391)
                     /gene="trpL"
                     /locus_tag="OO572_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6660242.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8529..9410
                     /gene="yciV"
                     /locus_tag="OO572_001166"
     CDS             8529..9410
                     /gene="yciV"
                     /locus_tag="OO572_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6660243.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9407..10027
                     /locus_tag="OO572_001167"
     CDS             9407..10027
                     /locus_tag="OO572_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6660244.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10128..11000
                     /gene="rluB"
                     /locus_tag="OO572_001168"
     CDS             10128..11000
                     /gene="rluB"
                     /locus_tag="OO572_001168"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6660245.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(11040..11630)
                     /gene="cobO"
                     /locus_tag="OO572_001169"
     CDS             complement(11040..11630)
                     /gene="cobO"
                     /locus_tag="OO572_001169"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6660246.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(11627..12385)
                     /locus_tag="OO572_001170"
     CDS             complement(11627..12385)
                     /locus_tag="OO572_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6660247.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12605..13654
                     /gene="sohB"
                     /locus_tag="OO572_001171"
     CDS             12605..13654
                     /gene="sohB"
                     /locus_tag="OO572_001171"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6660248.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13690..13941)
                     /locus_tag="OO572_001172"
     CDS             complement(13690..13941)
                     /locus_tag="OO572_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6660249.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14321..16918
                     /gene="topA"
                     /locus_tag="OO572_001173"
     CDS             14321..16918
                     /gene="topA"
                     /locus_tag="OO572_001173"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6660250.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17128..18102
                     /gene="cysB"
                     /locus_tag="OO572_001174"
     CDS             17128..18102
                     /gene="cysB"
                     /locus_tag="OO572_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6660251.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            18397..18561
                     /locus_tag="OO572_001175"
     CDS             18397..18561
                     /locus_tag="OO572_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6660252.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            18564..18731
                     /locus_tag="OO572_001176"
     CDS             18564..18731
                     /locus_tag="OO572_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660253.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            18845..18940
                     /locus_tag="OO572_001177"
     CDS             18845..18940
                     /locus_tag="OO572_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660254.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19104..21779
                     /gene="acnA"
                     /locus_tag="OO572_001178"
     CDS             19104..21779
                     /gene="acnA"
                     /locus_tag="OO572_001178"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6660255.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(21843..22433)
                     /gene="ribA"
                     /locus_tag="OO572_001179"
     CDS             complement(21843..22433)
                     /gene="ribA"
                     /locus_tag="OO572_001179"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6660256.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            22603..23367
                     /gene="pgpB"
                     /locus_tag="OO572_001180"
     CDS             22603..23367
                     /gene="pgpB"
                     /locus_tag="OO572_001180"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6660257.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            23516..23824
                     /gene="lapA"
                     /locus_tag="OO572_001181"
     CDS             23516..23824
                     /gene="lapA"
                     /locus_tag="OO572_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6660258.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            23831..25000
                     /gene="lapB"
                     /locus_tag="OO572_001182"
     CDS             23831..25000
                     /gene="lapB"
                     /locus_tag="OO572_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6660259.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            25193..25930
                     /gene="pyrF"
                     /locus_tag="OO572_001183"
     CDS             25193..25930
                     /gene="pyrF"
                     /locus_tag="OO572_001183"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6660260.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            25930..26256
                     /gene="yciH"
                     /locus_tag="OO572_001184"
     CDS             25930..26256
                     /gene="yciH"
                     /locus_tag="OO572_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6660261.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(26382..26600)
                     /gene="osmB"
                     /locus_tag="OO572_001185"
     CDS             complement(26382..26600)
                     /gene="osmB"
                     /locus_tag="OO572_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6660262.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(26869..27618)
                     /locus_tag="OO572_001186"
     CDS             complement(26869..27618)
                     /locus_tag="OO572_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6660263.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(27708..27881)
                     /locus_tag="OO572_001187"
     CDS             complement(27708..27881)
                     /locus_tag="OO572_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660264.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(28029..30003)
                     /gene="pdeR"
                     /locus_tag="OO572_001188"
                     /pseudo
     CDS             complement(28029..30003)
                     /gene="pdeR"
                     /locus_tag="OO572_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            30057..30160
                     /locus_tag="OO572_001189"
                     /pseudo
     CDS             30057..30160
                     /locus_tag="OO572_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30238..32172)
                     /gene="rnb"
                     /locus_tag="OO572_001190"
     CDS             complement(30238..32172)
                     /gene="rnb"
                     /locus_tag="OO572_001190"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6660265.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(32240..33367)
                     /locus_tag="OO572_001191"
     CDS             complement(32240..33367)
                     /locus_tag="OO572_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6660266.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(33511..34299)
                     /gene="fabI"
                     /locus_tag="OO572_001192"
     CDS             complement(33511..34299)
                     /gene="fabI"
                     /locus_tag="OO572_001192"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6660267.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(34667..35020)
                     /locus_tag="OO572_001193"
     CDS             complement(34667..35020)
                     /locus_tag="OO572_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6660268.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(35088..35894)
                     /gene="sapF"
                     /locus_tag="OO572_001194"
     CDS             complement(35088..35894)
                     /gene="sapF"
                     /locus_tag="OO572_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6660269.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(35896..36888)
                     /gene="sapD"
                     /locus_tag="OO572_001195"
     CDS             complement(35896..36888)
                     /gene="sapD"
                     /locus_tag="OO572_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6660270.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(36888..37778)
                     /gene="sapC"
                     /locus_tag="OO572_001196"
     CDS             complement(36888..37778)
                     /gene="sapC"
                     /locus_tag="OO572_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6660271.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(37765..38730)
                     /gene="sapB"
                     /locus_tag="OO572_001197"
     CDS             complement(37765..38730)
                     /gene="sapB"
                     /locus_tag="OO572_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6660272.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(38727..>40236)
                     /gene="sapA"
                     /locus_tag="OO572_001198"
     CDS             complement(38727..>40236)
                     /gene="sapA"
                     /locus_tag="OO572_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6660273.1"
                     /translation="TFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEV
                     LDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPY
                     FDSLQFADNVKSVRKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQ
                     EQLDRQPVGTGPYQLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLL
                     TGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALA
                     INNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLK
                     LWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGW
                     ATDSNDPDSFFRPLLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQ
                     SILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
BASE COUNT        10015 a         9981 c        10394 g         9846 t
ORIGIN      
        1 gtcaataagg acatggtata aagggtatta tttcttcgct tctacgccat cagtttcaga
       61 acgaattaaa aatttctcag ttgtttgcgg aatatgctga atcagccagt cggccatttg
      121 cttttcctca tttaaaatcg cttcgatgat cggaattgag actgtatcac cggcattttt
      181 tgctgctgct aatagtgagg tgtaacaggt gatttcaaat tgctcgaaga catatccgcc
      241 aatagagcct ttaactattt catcagaagg gaatatacca ccgattgact gcccaagcgc
      301 agccatttta ctcatggaat ctttaatgac tgaacgtgaa atgtcattac gatcaagaat
      361 agtttccagt tgaacaatct ggtttttggt ttcactaaga tgttgttcaa tacgagcgcg
      421 tagttcagga taattatcta tacggctggc catggattca agcatagatt cggcttgctt
      481 ttccattgcg tgggcgtcac gtaaccagtc atgataatgt tcaatacgat tcatgaaaat
      541 gtcctcttta taaaatgact aaaagttaaa attcatattt caggctttat tttcggcttt
      601 cttatttacg ttattaaggg ccagatcagt cagtttgatg tcggtggcct tttcttcttc
      661 cagggtttct ttcagaagct tcgctgcttt acggtaacct aattgttcag ccagcgtcgc
      721 taatgtcccg taactggcaa tctcataatg ctcgactttc tgtgctgcgg caatcagtgc
      781 ggcatcacgc acttcgtttt tctcggtact ttcgatgacc tcattagctt cttcaataag
      841 accttccatt gccacacatt tcatgcgctt aattttcaga tttgattctg attccacaac
      901 ttgatcaatt cgttcaatct gtccatgagt ttcctcgagg tgcgcatgaa aagcctgact
      961 taatttttca tttgatgttg ctcttgcgag ttttgccagt gcccgggtta attgtttttc
     1021 tgcgctgtag gtatctgaaa gcagttgaat aaatacatct tcaatggtct tcatattcat
     1081 atttttctcc agtgaaatca ctgcgagcca tacggcccgc agatgcaatt atctttatca
     1141 gctgatatta ataattaacg gtgaatcagg atttgcctga tttattacca ccgctttgtt
     1201 gaccgccttt tttacccgct tcagatgcgc gttgcggatc atttttaaaa ttaccgccgc
     1261 tatgctgacc gcctttacgg cctgcgtcgg atgccttctc acggtcttcg gcgaaatttc
     1321 ctgaactacc acgatgttcg gccatgttat ttctcccgtt gcgttgcatt gtttcattaa
     1381 tatgagtgtt gtgtgtcgac actcattaaa attagtcact aatgagaatt agtcaaatta
     1441 agcgcaacga gaagatagag ggaaatatat tttgaggaac attctggata tattaacaat
     1501 tacctgagga atatgtgact tagagaaaat tattgatttt actggtgtta tgttgcggga
     1561 ttaatttgtt tgattatatt actgttgggc ggaaaaatga cgtaagttga cgttcgaccg
     1621 gggtaagcga aacggtaaaa agataaatat taaatgaatt taggattttt ccggcttcat
     1681 taaagaaagt taaaatgccg ccagcggaac tggcggctgt gggattaact gcgcgtcgcc
     1741 gctttcatcg gttgtacaaa aactttcagt gccgccagca ttttctctgg ctcattaata
     1801 tgttgctcga tgattttaac aatggccgaa ccagaaatcg cgcccgcagc tcctgcatca
     1861 atcgctgctt ttacctgatc cggggcggaa ataccaaacc cctgcaatgg aggtgcagcg
     1921 ttgtactctt tcagcttcgc aaccagatga ttgaggggta acgcggcgcg gttttctgcg
     1981 ccggtcacgc ccgctcgtga cagcaaatat gtgtaaccac gaccgtaaga ggctatctgg
     2041 cgcagcaggt cgtcatcggc atttggcggg cagatgaaga taggtgcgac attatggcgc
     2101 aacgcggcct ggcggaaggg cgcggactct tcaactggca catcggcaac cagcaccgaa
     2161 tcgacgccga ctttctcgca ctgggcataa aactcatcaa tgcctttgtt aaacaccaga
     2221 ttggcataca tcaacaggcc aatgggaatg gtcgggtgtt tctggcgaat cagtgccagc
     2281 atttcaaaac attgtgccgg agtcacacct gccgcaaagg cgcgcagagt ggcgttttga
     2341 atggtcgggc catccgccag tgggtcggag aaggggatac ctaactccag cgcgtcagca
     2401 ccggcttcaa ttagcgtatc gataattttc aatgactgct caatgcccgg atcaccgagc
     2461 gtgacgaaag gaacgaatgc gccttctttg cgctccttca actgggcaaa cagagattcg
     2521 tagcgttcca tcagatttcc cctcgtgctt tcaaaatatc gtgaacggtg aagatgtctt
     2581 tatcgccgcg accggaaagg ttaaccacca gtagctgctc tttatccggg ttttcgcgca
     2641 tcattttcag cgcatgggcc agggcgtggg aggattccag cgccgggatg atcccttcgt
     2701 gcaggcacag cgttttgaag gcttcaaggg cttcatcatc ggtaatagac acgtaatcag
     2761 cgcgtccagt gctgttaaga tacgcgtgtt gtggaccgac agacgggaaa tccagtccgg
     2821 cggagatgga gtaagattct tcaatctgcc cgtcttcggt ttgcatcatc ggcgctttca
     2881 taccgaaata aatgcccacg cgaccatgtt ttagcggtgc gccgtgctcg ccagtttcga
     2941 taccgtgacc accaggctcc acaccaatca ggccgacgtt ggtttcattg atgaaatcag
     3001 caaacatgcc gatggcattc gaaccgccgc caacacaggc gataacggca tccggcaggc
     3061 gaccttctct ttccagaatc tgcgctttgg tttcttcgcc aatcatccgc tgaaactcac
     3121 ggacaatggt cggataagga tgcgggccag ccgcggtgcc cagcatatag tgcgcggttt
     3181 cataactgcc ggaccagtcg cgtagcgcct cattacaggc atctttcagc gtggcggaac
     3241 cgctatgtac cgggatcact tccgcaccca ttaagcgcat acgaaaaacg ttaggcgact
     3301 ggcgttcaac gtctttggca cccatataaa tacggcattt caggccgagc agggcgctgg
     3361 caagggccga cgccacgcca tgctgaccgg ccccggtttc ggcgatgatt tcggttttac
     3421 ccatccgctt cgccagcaac gcctgcccca gcacctggtt ggttttatgt gcgccgccgt
     3481 gcagcaaatc ttcacgcttg agatacagcg tggtgttcgt cccggctgta atgttctggc
     3541 atttggtcag tgcggttgga cgcccggcat agtttttcag caggtcgttg aactgggctt
     3601 gaaattcagg atctttttgc gcgctgacaa aagcttcttc cagctggcgc agagcaggca
     3661 tcaggatttg tggcacgtac atgccgccaa actcaccaaa ataggggtta agtaatgttg
     3721 tcattgttcc tttccttaat atgcgcgcag cgtctggaaa accgaggcca aaagacgtgc
     3781 gtctttgatg cccggttgcg actctacagc agaattaaaa tcaagtccgg cgcagccggt
     3841 ttgtgccgct tccacgcagt tatctgcgcc taagcccccc gccagcagaa cgttgccaag
     3901 cgattgacca tttaatagtg accagtcaaa acgttgcccg cttccaccct ggccgttgtc
     3961 taaaacatat ttatcaacgt gctgaaactc gcgggcgggc agggtttcac cgacgcttaa
     4021 tgctttccag atggcaacat gtgctggcag agcttcacgc agcgtatcga tatacagctg
     4081 atcttcattg ccatgcagtt gcactgccgc cagcgataac accttagctt tgtccaccac
     4141 atcggcaata tcgtgattgc ggaacacgcc aacatactgc aacggtgccg cagccatcac
     4201 ttcctgcgcc tgttcaacgt tgacgcaacg cggtgatgtc gcaacaaaaa tcaacccacc
     4261 gtaaatcgcg cccgcgtcat aagctgcttt agcatcttgc ccacgcgtca ggccacatac
     4321 tttattctca cccagcaaca cccggcgcac ggcggcgtgc aaatcgtcat gggccatcaa
     4381 cgccgaacca atcagaaaac cgttagcgaa gtggcttaac tcgcgcacct gagcgtaagt
     4441 attgatgccg gattcgctga ttaccgttac gttgtgcccc agtttcggcg caagctcgcg
     4501 ggtacggttg agatcaatcg acaaatcacg cagatcgcgg ttgttgatgc caacgacctt
     4561 tgcccctaat gcaatggcgc gctccagttc ctcttcatta ctgacttcgg tcagcacccc
     4621 catctccaga ctgtgagcga cggctgcaag ctggcgatat tggtcgtcat ccagtactga
     4681 aagcattaat aagcaggcat cggcctggta atagcgcgcc agatagatct ggtaagggtc
     4741 gataatgaag tctttacata aaatcggctg cggggcgatt tggctgacga tgaggaggaa
     4801 atcaaagctc ccctgaaaat atttctcatc agtcagcacc gaaattgccg aagcgtaatg
     4861 tttataaatg gcggcaatgc gtgccggatc gaaatcatca cggatcacgc cttttgacgg
     4921 cgacgctttt ttacactcca gaataaacgc cgtgcgtgca ccctgtagcg catcataaaa
     4981 atgtcgtgtg ctcggctgaa cctcattctg aaaactggcc agcggttgct gctgtttgcg
     5041 ggtttctacc caaatcgcct tgtctgcgac gattttcgct aaaacggttt gcatcattta
     5101 ccctcgtgcc gccagcgcgg taactctgtc gtaagcggaa ccactgcgca gtacctcaag
     5161 aacggtttgc gcattggctt gcagatcttc atggccatgc aggcgcatta acatggcgac
     5221 gttcgcagcg acggctgctt catgggcggc gtcgccttta ccttgtaaca agcgtgttaa
     5281 aatgtcacgg ttttcttcag gtgttccgcc tgccagttgc tcctggtggt agggagtcag
     5341 gccaaaatct tcagcggtca attgatagct cttaattttg ccgtcatgca gctcggcaac
     5401 gattgtcggc gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca ccgccgcgcg
     5461 ttgatacccc agcacgcgca aggtttcggc aatcggcagc accagttccg gactataaac
     5521 accaattaac gccagcggcg gatgcgccgg gttaatcaat ggccccagca cattgaacag
     5581 ggtgcgggtt ttcagttgct ggcgaaccgg catcgcgtgg cggaatccgg tgtgatattt
     5641 cggcgcaaag aggaaacata cgcctaactc gtccagcgcc tggcgcgatt tattggcgtt
     5701 catatcaaga ttaataccga atgccgccag cagatccgac gagccggatt tactggagac
     5761 gctgcggtta ccgtgtttcg ccactttcag cccacaggcc gcggcgacaa acgcactggc
     5821 ggtagaaata ttgatactgt tgctgccgtc accgccagta ccgacgatat cagcaaacag
     5881 ataatccggg cgcgggaacg gcgctgcgtt ttccagtagc gcggttgctg ccccggcgat
     5941 ctcgttcggg tgctcaccgc gaattttcat gctcaccagc gccgccgcca gttgttccgg
     6001 cttcagctcg ccacgcacca ccgctgaaaa cagctggtgg ctttcttgtt ggctaagcgt
     6061 ctgcgcctga tacagttttt ccagaatcgg ttgcagcgtg ttggctggct ctagtttctg
     6121 ctgcgcccag gccagcgttt gttccagcag gcgagcgccc tgggtggtga gaatggattc
     6181 cggatggaac tggaatccac aaacgcgatc cgcatcgtga cgtactgcca tcaccatgcc
     6241 attaaaatgg gcgttgatgg ttaaaccggc cggaatgtta ctgccaacca gcgagtgata
     6301 acgcgccacc ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt catgttcaat
     6361 gctcgacgct ttaccgtgaa gaatttcgcc cgcctgaccg acatagcccc cgtaagcttc
     6421 gacaattgcc tgatgtccga ggcaaatgcc aataatgggc agcttgccac gcaagcgggt
     6481 gaggagttcc ggcatacaac cggcttcgct cggcacaccg gggccaggag aaagcatcag
     6541 taccggattg ctcatcgtcg ccaggcgttc aattaaggtc tgcgccggaa tatggttgcg
     6601 gtaaatcacc acgttatgac cattgctgcg caactgatct gccaggttgt acgtaaaaga
     6661 gtcgatatta tcgagcagca gaatgtcagc catcagaaag tctcctgtgc atgatgcgcg
     6721 gtggcaatag cgcgcagtac agcgcgggct ttgttacggg tttcgtcggc ttccgactgc
     6781 ggaacagaat caaggactac accagcaccc gcttgcacgg tggcgatacc gttttccacc
     6841 agcgccgagc ggatcacaat gcaggtgtcg agatcgccat gcgcggtgaa ataacctacc
     6901 gcgccgccgt agctgccgcg gcgacgacct tccgcctcgg caattaactg catagcgcgt
     6961 actttcggcg caccgcttaa cgtccccata ttcatacagg cgcgataagc gtgcagggcg
     7021 tcaagatcgt gacgcagttc gccgactacg cgagagacga ggtgcatcac ataggaataa
     7081 cggtcaactt tggtgagatc ggcgatgtag cggctgccag gggtgcaaat gcgtgccaga
     7141 tcattacggg cgagatcaac cagcatcaga tgttcagaca gctctttatg gtcagtacgc
     7201 atttccagtt caatacggct gtcgagatct ctgtccagtg aaccatcggc gcgacgaccg
     7261 cgtgggcgtg ttccggcaat cgggtagatc tcaatctggc ggctggtggc atcatacttg
     7321 agcgagcttt ccggcgacgc gccaaatagg gtgaaatcat tatcctgcat aaaaaacatg
     7381 tacgggctgg gattactctt tttcagcacg taataggccg ccagcggtga cgggcagggc
     7441 agagagaaac ggcgagatgg caccacctgg aaaatttctc cagcgcgaat cgctttttgc
     7501 aacaaacgca ctacgccacc gaactcttca tcgctctgat tacattcaca acgcatatgc
     7561 ggcacggaaa ccactggcag cggcggcgcg gcttcggtca gttgctgacg tagttcgttc
     7621 aggcgagcag tgagacgttg tttttcttct tcattcggag caaacaggct ggcctgaata
     7681 cgggtgcttt ttttctgatg gtcaatcacc atcagcgttt cagcgagata aaaacagaaa
     7741 tcagggcagt tattttccgc tgacagttgc ggtaaatctt caaatcccgc cacaaggtca
     7801 taagagaaca ggccgccgaa gaacattgct tctcgttctt ccttcggtac attcaacaga
     7861 ttctgcaata aacggaaagc gtcaaaaacc gaaagggagc ataagcgggc gtcttcatcc
     7921 agcagtggac tgacaggggg gaagcgcagc acacggcagt ttggtgattg ttcactttcc
     7981 acacccgcag gcagggcgtt atccagtagt gccagcaggg cttcgccatt gccggaaagt
     8041 gcctgaattg tgacggtgtc acctaatgct gtaatgcgca gcgcactgtc tatcagcagc
     8101 aggcttttta aatcatcttt gctgtcgata tctgcggatt ccagcagcag cgttgccgga
     8161 cgattcccac acaactggtg aaaaagcgca gtcgggttgt cgcgataagc gccttcgcag
     8221 gttagcagtt cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt ttgttcaaaa
     8281 aaaagcccgc tcattaggcg ggctgggtat ctgattgctt tacgcatggt gaatacactg
     8341 cccgtttcag gaagtgcgcc accaaccttt cagtacgaaa attgctttca ttgtcgatac
     8401 cctttttacg tgaacttgcg tactagttaa ctggttcgat gattaattgt caacagctca
     8461 tttcagaata tttgccagac ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
     8521 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
     8581 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag atgcgcgtcg
     8641 gcaccctggc gatcaccgac catgacacca cagctgcaat cgcgccagca agagaggaaa
     8701 tttcacgttc gggactggcg ctgaatctta ttcccggcgt ggaaatttcc acggtctggg
     8761 aaaatcatga aattcatatt gtcgggctga atattgatat tactcatccg ctaatgtgtg
     8821 agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagcaaatt gccgaaagac
     8881 ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcaca acgactggcg cagggcggcg
     8941 cagtgacgcg cggtcatttt gcgcgtttcc ttgtggagtg cggcaaagcc agttcaatgg
     9001 cagatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg atacgttccg ccacagtggt
     9061 gtacaataga acaagctatt gatgtcattc atcattctgg cggtaaggcg gtgctagctc
     9121 atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg cattttgccg
     9181 aacaccacgg cgacgcgatg gaagtcgcgc agtgccagca atcgcccaat gaacgcaccc
     9241 agctggccac acttgcgcgt cagcatcatt tatgggcatc acaaggatct gattttcatc
     9301 agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc gttgaaggcg
     9361 tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga gccagttttt
     9421 ttatattcat cctgataacc cgcagcaacg tctgatcaac caggcggtgg agatcgtgcg
     9481 taaaggcggg gtgattgtct atccaactga ttccggctat gcgctcggct gtaaaattga
     9541 agataaaaac gccatggagc gtatttgtcg tattcgccag ctgccggacg gtcacaactt
     9601 taccctgatg tgtcgcgatc tttctgaact ctcgacctat tcattcgtgg ataacgttgc
     9661 gtttcgtttg atgaaaaaca acacgccggg caactatacc ttcatcctga aggggacgaa
     9721 ggaagtgcca cgccgtctgt tgcaggagaa acgcaaaacc atcggtatgc gtgtgccttc
     9781 taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa ccgatgcttt ccacttcgct
     9841 aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa gaaattaaag accgtctgga
     9901 aaaacaggta gatttgatta ttcacggcgg ctatctcggg cagaaaccaa caacggttat
     9961 tgatcttacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg tgaagccttt
    10021 cttataagtg cgtaactctg tatactacgc ggccttaaaa tggcgctata cggcgccatt
    10081 gattctcctg ttcgacgcct gtgaaggcga caccaaaagg aagctctatg agcgaaaaat
    10141 tacagaaagt gctggcccgc gctggccacg gctcccgccg tgaaatcgaa tctattattg
    10201 aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc gttgaagtta
    10261 cccctgggct gaaaattcgt atcgatggtc atctgatttc ggtacgtgag tccgctgaac
    10321 aaatttgtcg cgttctggcc tattacaaac cggaaggtga gttgtgtacc cgtaacgatc
    10381 cggaagggcg tccgacggtg tttgaccgtc tgccaaagct gcgtggcgca cgctggattg
    10441 ccgtaggtcg tctggacgtt aacacctgtg gtctgctgct gttcaccacc gatggtgaac
    10501 tggcaaaccg tttaatgcat ccaagccgtg aagttgaacg tgaatacgcc gtgcgtgtat
    10561 ttggtcaggt tgacgacgcg aaactgcgtg atttgagtcg tggcgtgcaa ctggaagatg
    10621 gtccggcagc ttttaaaacc atcaagttca gcggcggcga agggatcaac cagtggtaca
    10681 acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa gcggttggtg
    10741 tgcaggtgag ccgcctgatc cgtgttcgtt acggtgatat cccactgcca aaaggtctgc
    10801 cacgcggtgg ctggacggag ctggacctcg cccagactaa ctatctgcgc gaactggtgg
    10861 agcttccgcc agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt cgcatgaagg
    10921 ctaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcagt cgtcgttctg
    10981 gcgggcgtaa taacggttaa tcaaagtatg ccggacgtca tatccggcat ttttacagat
    11041 taataatcaa tccctatttg cgccttaaca ccggcatcaa acgcatgtct gatggggcgc
    11101 agctcactta ccgtatccgc cagttcaaga atatcccgat gacagcctcg acccgtgata
    11161 atcaccgtct gttgatgtgg acgttcattt aacgcctgca ccacttcttc cagtggcaaa
    11221 tagtcatacg ccaccatata cgtcagttca tcaagcaaaa ccatatccag tgaggaatca
    11281 gcaagcatcc gctttgcatg ttgccagact tcgcggcagg cggcggtatc agactcgcgg
    11341 ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc atgtggctcc
    11401 agcagattgc gttcgccatt aggccaggtg cctttaataa actgcacgac gcccactttt
    11461 tttccgtgac caactgcgcg tgttgccgta ccaaatgccg cggtggtttt gccttttcca
    11521 ttgccggtaa agacgatgat aataccgcgt tcatcctggg cctgggccac acgagcatct
    11581 actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat tgggaaattc
    11641 ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg ctgtcatcgc
    11701 ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc tgtggatctt
    11761 cggtcgggaa ggcgcttgcg cgcattgcgg tgcgcgtacc gcctgggtta atgcagttga
    11821 cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg gtggcaaatt
    11881 tcgacgctgc atatgcaccc cagttggctc gtccctgacg tccaacgctt gatgaagtaa
    11941 agaccagtga accggcgtcc gatttgagta ataaaggaag cagtgcctgg gtgagcataa
    12001 aggtggcatt aacgttgatc tgcatgacgt cctgccagac ctgcggattt tgttcgctca
    12061 ttgggcaaac atcgccgagc aatccggcat tatgcaaaac accatccaga cgcggataat
    12121 taacgacaat gcgctgtgcc agttgttggc aattttcgga cgtgcaggtc agcaaatcga
    12181 gaataaacca ctgtggctga cgcccagttt cttcgtttat gtggctggct acctgacgta
    12241 atttttcttc attacggccc aacagaatca ctgtcgcacc atagcgtgca tacgtcatcg
    12301 cggcttcacg accaatccca tcgctggctc ccgtcaccag gataatgcga tcattgagta
    12361 aatcttgttt tggctggtaa tgcatggcta ctcctcaacg acgttgtctg tcgcgctgcc
    12421 agtgtacttt atgactttgg ggctttatgc ctgaaacaga agggcatttc aatcagcctg
    12481 gggcgataac accgcaaaat ggatactttg tcatactttc gctgcaataa catctctgcg
    12541 agacggctta acatgcctgt tgtaaactgt gagccaaagc gttgtttaac caaggtgggg
    12601 actcgtggaa ttgttgtctg aatatggttt gtttttggct aaaatcgtta ccgttgtgct
    12661 agcgattgcg gcgattgccg ccattattgt caatgttgct caacgtaata aacgccagcg
    12721 tggcgagtta cgggtcaaca atctcagcga acagtataag gagatgaaag aagaactggc
    12781 cgcggcgctg atggactcac atcagcaaaa acagtggcac aaagcgcaga agaaaaagca
    12841 caagcaagaa gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgg caactgacag
    12901 taaacctcgc gtctgggtgc tggattttaa aggcagcatg gacgcccatg aagtgaactc
    12961 gctacgtgaa gagataacgg cggtactcgc agcattcaaa ccgcaggatc aggttgtgct
    13021 ccgtctggaa agccctggtg gcatggtgca tggttacggg ttggcggctt cgcagctgca
    13081 gcgtctgcgt gataaaaaca ttcctttaac tgttacggta gacaaagtcg ctgccagcgg
    13141 cggttacatg atggcctgtg tggcggacaa aattgtttcc gcaccgtttg ctattgtggg
    13201 ttccattggg gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa gcaaagatat
    13261 tgatatcgaa ctgcacaccg ccgggcagta taagcgcacg ctgaccttgc tgggtgaaaa
    13321 taccgaagaa gggcgggaga aattccgcga agagctgaac gaaacgcatc agttatttaa
    13381 agattttgtg aagcgtatgc gtccgtctct ggatattgaa caggtggcaa cgggtgaaca
    13441 ctggtacgga caacaggcgg tagagaaagg cctggttgat gaaatcaaca ccagtgatga
    13501 agttattctt agcctgatgg aaggccgtga agtggttaat gtacgctata tgcagcgtaa
    13561 acgactcatt gaccgattca ccggcagcgc ggcagagagc gccgatcgat tgttgctacg
    13621 ctggtggcag cggggtcaaa agccattgat gtaaaagaca aacgcgaggc taagacctcg
    13681 cgttttgctt taatcaacca gatgatattt ttctgaaagc acatgggcca ggtgtttgaa
    13741 catattaaac accgcggtgc ttttggctgt tggcaatcct tgttcatcta aaaagtagtc
    13801 gccggtaaat accagcacgc cattacgctg cgtgacgccg gtggcttcaa tccctgcgag
    13861 cgtatcctca tgctcacgaa tgattttgtt ggcctctttc aacagcgttt cgcggtcgat
    13921 gggttgtgtc tctttgttca ttatttactc cttaaacaag gacattagtc tacgccaggc
    13981 atggcttgca gacaaatata ccacgctggt ggcaagagcg ccttactggc aactttggat
    14041 tttgcatgct aataaagttg cgtatcggat tttatcaggt acagtgtgac gctttcgtca
    14101 atctggcaat agatttgctt gacattcgac caaaaatccg tcgtgctata gcgcctgtag
    14161 gccaagacct gttaactcag tcacctgaat tttcgtgaac agagtcacga caaggggttg
    14221 atatccgcag agagcgagtc catatcggta actcgttgcc agtggaaggt ttatcaacgt
    14281 gcgacgcatt cctggaagaa tcaaattagg taaggtgaat atgggtaaag ctcttgtcat
    14341 cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggttgtg actacgtggt
    14401 gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg ccaaaaagag
    14461 tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac gtggcgctct
    14521 cgtcaaccgt atgggggttg acccgtggca caattgggag gcgcactatg aagtgttgcc
    14581 tggtaaagag aaggtcgtct ctgaactgaa acaactggct gaaaaagccg accacatcta
    14641 tctcgcaacc gaccttgacc gcgaagggga agccattgca tggcacctgc gggaagtgat
    14701 tggtggtgat gatgcgcgct atagccgagt ggtgtttaac gaaattacta aaaacgcgat
    14761 ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg cccagcaggc
    14821 gcgtcgcttt atggaccgcg tggtggggta tatggtttcg ccgctgctgt ggaaaaagat
    14881 cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg gtccgcctgg tggtagagcg
    14941 tgagcgtgaa attaaagcgt tcgtaccgga agagttctgg gaagtcgatg ccagcacgac
    15001 tacgccatct ggtgaagcgt tggagttgca ggtgactcat cagaatgaca aaccgttccg
    15061 tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag cgcgctacag
    15121 cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt ttattacctc
    15181 tacgctgcaa caagctgcca gcacccgtct tggttttggc gtgaaaaaaa ccatgatgat
    15241 ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact ccactaacct
    15301 gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg gtaagaaata
    15361 tctgccggaa agtccgaatc agtacgccag caaagaaaac tcacaggaag cgcacgaagc
    15421 gattcgtcct tctgacgtca atgtgatggc ggaatcgctg aaggatatgg aagcagatgc
    15481 gcagaaactg taccagttaa tctggcgtca gttcgttgcc tgccagatga ccccagcgaa
    15541 atatgactcc acgacgctga ccgttggtgc gggcgatttc cgcctgaaag cacgcggtcg
    15601 tattttgcgc tttgatggct ggacaaaagt gatgcctgca ctgcgtaaag gcgatgaaga
    15661 tcgtatctta ccggcagtcg ataaaggcga tgctctgacg ctcgttgaac taacaccagc
    15721 ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta aagaactgga
    15781 aaaacgtggt atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca ttcaggatcg
    15841 tggctatgtg cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg aaatcgtcac
    15901 cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac gactttaccg cgcagatgga
    15961 aaacagcctc gaccaggtgg caaatcacga agcagagtgg aaagctgtac tggatcactt
    16021 cttctcggat ttcacccagc agttagataa agctgaaaaa gatccggaag agggtggtat
    16081 gcgcccgaac cagatggttc tgaccagcat tgactgcccg acttgtggtc gcaaaatggg
    16141 gattcgcaca gcgagtaccg gggtattcct tggctgttct ggctatgcgc tgccgccgaa
    16201 agagcgttgc aaaaccacca ttaacctggt gccggaaaac gaagtgctga acgtgctgga
    16261 aggcgaagat gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccga aatgcggcac
    16321 ggcgatggac agctatctca tcgatccgaa acgtaagttg catgtctgtg gtaataaccc
    16381 aacctgcgac ggttacgaga tcgaagaggg cgaattccgc attaaaggtt atgacggccc
    16441 gatcgttgag tgtgaaaaat gtggctctga aatgcacctg aaaatggggc gtttcggtaa
    16501 atacatggcc tgcaccaacg aagagtgtaa aaacacacgt aagattttac gtaacggtga
    16561 agtggcacca ccgaaagaag atccggtgcc attacctgag ctgccgtgcg aaaaatcaga
    16621 tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc ctggctgcca acactttccc
    16681 gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt tatcgcttcc gcgaccgtct
    16741 gccggaaaaa ctgcgttatc tggccgatgc gccacagcag gatccggaag gtaataagac
    16801 catggttcgc tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa aagacggaaa
    16861 ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggaa aaaaataacc
    16921 tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt tttattgcat
    16981 attatttttt cttcacacct atacactaag gctataaatg atatagtggt tatagttagc
    17041 acctttttta ttattaaatc gtattagtca cccgccaggt gtgacgaaaa aacgatgttc
    17101 tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta tattgttgag
    17161 gtggtcaatc ataacctgaa tgtctcatca acagcggaag gactttacac atcacaaccc
    17221 gggatcagta aacaagtcag aatgctggaa gacgagctag gcattcaaat tttttcccga
    17281 agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg tatcgctcgc
    17341 gaagtcctgt cgaaagtcga tgccataaaa tcggttgccg gtgagcacac ctggccggat
    17401 aaaggctcgc tgtatatcgc caccacgcat acccaggcac gctacgcgtt accaaacgtt
    17461 atcaaaggct ttattgagcg ttatccccgc gtttctttgc atatgcacca gggcccgccg
    17521 acacaaattg ctgatgccgt ctctaaaggc aatgccgatt tcgctatcgc cacagaagcg
    17581 ctgcatctgt atgaagattt agtgatgtta ccgtgctacc actggaatcg ggctattgta
    17641 gtcactccgg atcacccgct ggcaggcaaa aaagccatta ccattgaaga actggcgcaa
    17701 tatccgttgg tgacatatac cttcggcttt accggacgtt cagaactgga tactgccttt
    17761 aaccgcgcag ggttaacgcc gcgtatcgtt ttcacggcaa cggatgctga cgtcattaaa
    17821 acttacgtcc ggttagggct gggggtaggg gtcattgcca gcatggcggt ggatccggtc
    17881 gccgatcccg accttgtgcg cgttgatgct cacgatatct tcagccacag tacaaccaaa
    17941 attggttttc gccgtagtac ttttttgcgc agttatatgt atgatttcat tcagcgtttt
    18001 gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc caatgaagaa
    18061 attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc gttattttcg
    18121 gcacctttta tgtagcgaag gtgccggaat atattctctt ttgttactta tttctatacc
    18181 gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag tagtgtctaa
    18241 ttacccgcct cgcaaattcc agttttcaaa tatctatttc aggtcaaaag attgaattat
    18301 tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt aggatttgtc
    18361 tcatcgtatg attaattaca ccaatcattc aacgtcttgc tgataagtga tggcgattat
    18421 atgaggttag caatgccttc tggaaatcag gaaccccgcc gtgatcctga attaaaacgt
    18481 aaagcctggc tggcggtttt tcttggttct gcacttttct gggtggttgt cgcactgctg
    18541 atttggaaag tgtggggata actatggtcg gtcaggagca actggagtcg tcaccattat
    18601 gccagcatag tgacaatgaa ccagaaacga aacgggaatg ttccgtcgtt attccagacg
    18661 actggcaact aacatcgcag cagcaagcct ttatagaact gtttgctgaa gatgatcagc
    18721 cgaaacaata attatcatca ttcttattac ccatttttaa tgaattaaag ggcttttaat
    18781 acaccgcagc aataacagct tgagttatct caacacaaaa taataaccgt taagggtgta
    18841 gcctatgatc aacacaaata tgaaatattg gtcctggatg ggcgcgtttt ctctgtcgat
    18901 gctcttctgg gccgaactcc tctggatcat tactcactga tccttgaccc cgctgcggcg
    18961 gggttgtcat ttgctttgcc acaaggtttc tcctctttta tcaatttggg ttgttatcaa
    19021 atcgttacgc gatgtttgtg ttatctttaa tattcaccct gaagagaatc agggcttcgc
    19081 aaccctgtca ttaaggagga gctatgtcgt caaccctacg agaagccagt aaggacacgt
    19141 tgcaggccaa agataaaact taccactact acagcctgcc gcttgctgct aaatcactgg
    19201 gcgatatcac ccgtctaccc aagtcactca aagttttgct cgaaaacctg ctgcgctggc
    19261 aggatggtaa ctcagttacc gaagaggata tccacgcact ggcgggatgg ctgaaaaatg
    19321 cccatgctga ccgtgaaatt gcctaccgcc cggcaagggt gctgatgcag gactttaccg
    19381 gcgtacctgc cgttgttgat ctggcggcaa tgcgcgaagc ggttaaacgc ctcggtggcg
    19441 atactgcaaa ggttaacccg ctctcaccgg tcgacctggt cattgaccac tcggtgaccg
    19501 tcgatcgttt tggtgatgat gaggcatttg aagaaaacgt acgcctggaa atggagcgca
    19561 accacgaacg ttatgtgttc ctgaaatggg gaaagcaagc gttcagtcgg tttagcgtcg
    19621 tgccgccagg cacaggcatt tgccatcagg ttaacctcga atatctcggc aaagcagtgt
    19681 ggagcgaatt gcaggacggt gaatggattg cttatccgga tacactcgtt ggtactgact
    19741 cgcacaccac tatgatcaac ggccttggcg tgctggggtg gggcgtgggt gggatcgaag
    19801 cagaagccgc catgttaggc cagccggttt ccatgcttat cccggacgta gtgggcttta
    19861 aacttaccgg aaaattacgt gaaggtatta ccgccacaga cctggttctc actgttaccc
    19921 aaatgctgcg caaacatggc gtggtgggga aattcgtcga attttatggt gatggtctgg
    19981 attcactacc gttggcggat cgcgccacca ttgccaatat gtcgccagaa tatggtgcca
    20041 cctgtggctt cttcccaatc gatgcagtaa ccctcgatta catgcgttta agcgggcgca
    20101 gcgaagatca ggtcgagttg gtcgaaaaat atgccaaagc gcagggcatg tggcgtaacc
    20161 cgggcgatga accgattttt accagtatgt tagaactgga tatgaatgac gttgaagcga
    20221 gcctggcagg gccaaaacgc ccacaggatc gcgttgcact gcccgatgta ccaaaagcat
    20281 ttgccgccag taacgaactg aaagtgaatg ccacgcataa agatcgcctg ccagtcgatt
    20341 atgttatgaa cggacatcag tatcagttac ctgatggcgc tgtggtcatt gctgcgataa
    20401 cctcgtgcac caacacctct aacccaagtg tgctgatggc cgcaggcttg ctggcgaaaa
    20461 aagccgtaac tcttggcctc aagcggcaac cgtgggtcaa agcgtcgttg gcaccgggtt
    20521 cgaaagtcgt ttctgattat ctggcaaaag cgaaactgac accgtatctc gacgaactgg
    20581 ggtttaacct tgtgggatac ggttgtacca cctgtattgg taactctggg ccgctgcccg
    20641 atcctatcga aacggcaatc aaaaaaggcg atttaaccgt cggtgcggtg ctgtccggca
    20701 accgtaactt tgaaggccgt atccatccgc tggttaaaac taactggctg gcctcgccac
    20761 cgctggtggt tgcctatgcg ctggcgggaa atatgaatat caacctggct tctgagccta
    20821 tcggccatga tcgcaaaggc gagccggttt atctgaaaga tatctggcca tcggcacaag
    20881 aaattgcccg tgcggtagaa caagtctcca cagaaatgtt ccgcaaagag tacgcagaag
    20941 tttttgaagg cacagcagag tggaaggaaa ttaacgtcac acgatccgat acctacggtt
    21001 ggcaggagga ctcaacctat attcgcttat cgcctttctt tgatgaaatg caggcaacac
    21061 cagcaccagt ggaagatatt cacggtgcgc ggatcctcgc aatgctgggg gattcagtca
    21121 ccactgacca tatctctccg gcgggcagta ttaagcccga cagcccagcg ggtcgatatc
    21181 tacaaggtcg gggtgttgag cgaaaagact ttaactccta cggttcgcgg cgtggtaacc
    21241 atgaagtgat gatgcgcggc accttcgcca atattcgcat ccgtaatgaa atggtgcctg
    21301 gcgttgaagg ggggatgacg cggcatttac ctgacagcga cgtagtctct atttatgatg
    21361 ctgcgatgcg ctataagcag gagcaaacgc cgctggcggt gattgccggg aaagagtatg
    21421 gatcaggctc aagccgtgac tgggcggcaa aaggacctcg tctgcttgga attcgtgtgg
    21481 tgattgccga atcgtttgaa cgaattcacc gttcgaattt aattggcatg ggcatcctgc
    21541 cgctggaatt tccgcaaggc gtgacgcgta aaacgttagg gctaaccggg gaagagaaga
    21601 ttgatattgg cgatctgcaa aatctacaac ctggcgcgac ggttccggtg acgcttacgc
    21661 gcgcggatgg tagccaggaa gtcgtaccct gccgttgtcg tatcgacacc gcgacggagt
    21721 tgacctacta ccagaacgac ggcattttgc attatgtcat tcgtaatatg ttgaagtaac
    21781 aagtatttgc ttgccggtta ttttgcttcc ggcaagcaaa tgaattacac gatgcaagag
    21841 ggttatttgt tcagcaaatg gcccattttc tcggctttgg tatcgagata atgttcgtta
    21901 ttggggttac gacctacaat caatggtacg cgttcaacaa tattaatccc tgcttcggtc
    21961 agaatttcga cttttttcgg gttattggtt aacaagcgga cttcattgac gccaaggagt
    22021 ttgaacatat cagcgcaaag agtgaagtcg cgctcatcag cggcgaagcc taactggtgg
    22081 ttagcctcta cggtatcgta accttgatcc tgcagtgcgt aagcgcggat tttattcagc
    22141 agaccaatgt tacgaccttc ctgacggtga tacagcaaaa taccacggcc ttcctcggca
    22201 atttgcgtca atgccgcttc gagctggaag ccacaatcgc agcgcaagct gaacagggcg
    22261 tcaccggtca gacattcgga atggacgcgc gcaagtaccg gggtatgccc ggaaatatcg
    22321 ccatagacta gcgcaacatg atcgtgtccg gttgccagtt cttcaaatcc caccatcagg
    22381 aaatcgcccc atggggttgg cagtttggct tctgccacac gtttaagctg catgaaattc
    22441 tccagataat gctggttctg tattggctta ttttgccata acgagaaggg gttcacctaa
    22501 tcacgacgcg tcgatcgttc acggaatggc acaaatctgt caatttttct tggaactggc
    22561 attttcagtt atgattgtgg gacttatcaa aaaggagagg ccatgcgttc gattgcccga
    22621 cgtaccgcag tgggagctgc actattgctt gtcatgccag tagccgtatg gatttctggc
    22681 tggcgttggc aacctggaga acaaagttgg ctactaaaag cggctttttg ggttactgaa
    22741 actgtcaccc agccctgggg cgtcattaca catttgattt tgttcggctg gtttctctgg
    22801 tgtctgcgtt ttcgcattaa ggctgccttt gtattatttg ccattctggc ggcggcaatc
    22861 cttgttggac aaggcgttaa atcctggatc aaagacaaag tacaggaacc acgacctttt
    22921 gttatctggt tggaaaaaac acatcatatt ccggttgatg agttctacac tttaaagcga
    22981 gcagaacgcg gaaatctagt aaaagaacag ttggctgaag agaaaaatat tccacaatat
    23041 ttgcgttcac actggcagaa agagacgggg tttgcctttc cttccggtca cacgatgttt
    23101 gctgccagtt gggcactgct ggccgttggt ttgctgtggc cgcgtcggcg aacgttaacc
    23161 attgctatct tgttggtctg ggcaacggga gtcatgggaa gccgcctgct gctcgggatg
    23221 cattggccac gcgatctggt ggtagctacg ttgatttcgt gggcgctggt ggcggtggca
    23281 acgtggcttg cgcaacgaat ttgtgggcca ttaacaccac ctgcggaaga aaatcgcgaa
    23341 attgcacaac gagaacaaga aagttaacgc tggttgattt tccgaattta ggccttaaat
    23401 catcaacaat gcgcgtggat gccatttcgc agacggcgcg aaaatggtac tttaaagggc
    23461 tattgcggta agttgaccat aatttattcg ctctaaccac ataacgggaa gtaatgtgaa
    23521 atatttactc attttcttac tggtgttagc gatcttcgtg atttcggtca cgttgggtgc
    23581 gcagaacgat caacaggtga cgtttaatta tctgttagcg caaggggagt accgtatttc
    23641 cacattgctg gcggtattgt ttgctgcggg gtttgctatc ggttggttga tttgtggcct
    23701 gttctggctg cgagttcgtg tttccctggc gcgcgctgaa cgtaaaataa agcgactgga
    23761 aaaccagctt tcacccgcga ctgacgtggc tgtagtgccg cactcgtcag cggcgaagga
    23821 ataactttct atgctggagt tgttgtttct gcttttgcct gtagccgctg cctatggctg
    23881 gtatatgggc cgcagaagtg cgcaacaaaa caagcaagat gaagccaacc gcttgtcgcg
    23941 tgattacgta gcgggggtta acttcctgct tagtaatcaa caggataaag cggtagacct
    24001 gtttctcgat atgcttaaag aggatacagg caccgttgaa gcccacctta cgctcggaaa
    24061 cctgttccgt tcgcgtggcg aagttgatcg cgctattcgc atccatcaga ccctaatgga
    24121 aagcgcctcg ctgacctatg aacagcgtct gttggcgatt caacaactgg ggcgtgatta
    24181 catggccgcc gggttatacg accgcgcgga agacatgttc aatcagctga ccgatgaaac
    24241 tgacttccgc attggcgcgc tgcaacagtt gctacaaatc taccaggcta ccagtgagtg
    24301 gcagaaagca attgatgttg ccgaacgcct ggtgaagctg ggtaaagata aacagcgcgt
    24361 cgaaattgcc catttctact gtgagttagc cctgcagcat atggccagcg acgatctcga
    24421 tcgtgccatg accttgctaa aaaaaggggc ggcggcagat aaaaacagcg cccgcgtatc
    24481 catcatgatg ggacgcgtgt ttatggcgaa aggagaatac gccaaagccg tcgaaagttt
    24541 gcaacgcgtc atatcccagg acagagaact ggtcagcgaa acgctggaaa tgttgcaaac
    24601 ctgctatcag cagttgggta aaactgccga atgggcagaa ttcctgcaac gcgcggtgga
    24661 agagaacacc ggtgccgatg ctgaattgat gcttgctgat atcatcgaag cgcgcgacgg
    24721 tagtgaggct gcacaggtct atattacacg ccagcttcag cgtcatccga ccatgcgtgt
    24781 attccataag ctaatggatt accacttaaa tgaagcggaa gaagggcgcg ccaaagagag
    24841 tctgatggtg ctgcgtgaca tggttggcga gaaggtgcgt agtaagcctc gttatagctg
    24901 ccagaaatgt ggttttaccg catacaccct ctactggcat tgtccgtctt gtcgtgcctg
    24961 gtcaactatt aaaccgattc gcggtcttga tggcctgtaa tttttaaaaa aatccgactt
    25021 tagttacaac atactagtta ttgatgtccc attgtgctcc ggcaacgacg gagcagaaaa
    25081 agcctgctag gggaaaaatc gcaactgtta attttttatt ttcacgggta gaatgctcgc
    25141 agtttacctg tttcgcgcca ctcccggtgc ccatcatcaa gaaggtctgg tcatgacgtt
    25201 aactgcttca tcttcttccc gcgccgttac gaattctcct gtggttgttg cccttgatta
    25261 tcataattgt gatgacgcgc tgtcctttgt cgacaagatc gacccacgcg attgtcgtct
    25321 gaaggtcggt aaagagatgt ttacattgtt tgggccacag tttgtgcgcg aacttcaaca
    25381 gcgtggtttt gatatctttc ttgacctgaa attccacgat atccccaaca ctgcagcgca
    25441 cgctgtcgct gctgcagctg acttaggcgt gtggatggtg aatgttcatg cctccggtgg
    25501 ggcgcgtatg atgaccgcag cgcgtgaggc actggttccg tttggcaaag atgcaccgct
    25561 tttgattgct gtgacagtgt tgaccagcat ggaagccagc gacctggccg atcttggcgt
    25621 gacactgtca cctgcagatt atgcagaacg tctggcagca ctgacgcaaa aatgtgacct
    25681 tgatggtgtg gtgtgttctg ctcaggaagc tgtgcgcttt aaacaggtat tcggtcagga
    25741 gttcaaactg gttacaccgg gcattcgtcc gcaggggagt gaagctggtg accagcgccg
    25801 cattatgacg ccagaacagg cgttggcggc tggtgttgat tatatggtga ttggtcgccc
    25861 ggtaacgcaa tcggtagatc cagcgcagac actgaaagcg atcaacgcct ctttacagcg
    25921 gagtgcatga tgagtgattc caacagccgt ctggtctact caacggaaac cgggcgtatt
    25981 gatgagccca aagcggcccc agtacgccct aaaggcgatg gagcggtgcg tattcagcgt
    26041 cagaccagtg gacgtaaagg taagggcgtt tgcctgatta ccggtatcga tctcgatgat
    26101 gccgaactga caaaacttgc agcggaactg aagaaaaaat gcggctgcgg cggagcagtt
    26161 aaagatggag ttattgaaat ccagggcgat aagcgtgatt tattaaaatc actacttgaa
    26221 gcgaaaggga tgaaggtaaa actcgcaggc ggttaacata aaaagccacg gatatatccg
    26281 tggctttcga atattttact gtgtgtatta ttcagttatt tcgtactgaa cagaatcaag
    26341 cgtattataa ttgcgagtcg ataatcgaca tttatttacg attatttacc gacctggtga
    26401 ccaataacac caccgacagc tgcaccacct aatgtaccca acgtactgcc atcggtcagt
    26461 actgcaccgc ctaatgcccc tgcacccgcg ccgattgcgg tgttgcggtc ccgtttagac
    26521 cagttagaac aggcactcag agacattgcc aaagtaattg ccagaacagc cgcggtcatt
    26581 tttttgctcg ttacaaacat aatactctct cctgaattta tgattcacgg aagtaagctc
    26641 tctataacta taatagctaa gaataagcct ggtgaaatta ttccgtgaaa tctgctcgcg
    26701 cgcagggtta tatcacgcag gtgataatta cttcctgtta tatcgctgat aataatttta
    26761 tatcttgaga gtgttaataa caggtaaata gtcttaatta tcaaccagga atcatcttag
    26821 agcggatgat ttgccaaact gcaaatcatc cgtagagaag ggaaatggtt aaatgtcaac
    26881 gacgtgaacg gtgagtttac tgtgctcaag atgcatcaga tcgctggcgc gtattttcga
    26941 atcggtaatc acgcgattga atcgctcaac gggaccgatg gagtatgaat gtacagcacc
    27001 aaatttcgag ctgtcagtca ggactatcgc ttcgcactct ttttccagca cggcattgac
    27061 cacatcggtg cgcatcatat cgcgaccggt gaatccagtt tcaggttgcc agccatcaat
    27121 accaataaat gctttgctga aatgtacctg ctggatgcac tggcgtgtca aagggccaac
    27181 catactttcg ctttttttct ggtacacgcc accgagcaaa atgacttcac aaggcgcgtc
    27241 tttcagcaaa tgcgcgatgt agctgctgac cgtgatgata gtgacatttt tcttctgctc
    27301 ccccagagtc cgggcgagca gggcattgct gctgccattt tcgataaaga tggtttcgcc
    27361 cggttgaacc agtgacgcgg caaactcggc gagttcacgc ttcagcgtat agttgctcat
    27421 catacgggtt tcgacgtcat cactatcaag cgaaactgca aagccatgtg cacggcggag
    27481 gtaactcagt ttttcgaggg tgttgagatc ctggcgaatg gtaacttcag aaactccagt
    27541 ggcttttgcc agatcggtta cgctaacctg accctggtca atgaccatct gtagaatagt
    27601 ttgttgtcgg gaattcatcg ctgtaattct tataacgtta taatactaaa taaaaaaata
    27661 ctgacgcgcc gggcatcctc aaataggaaa acctgaaggc acgattgtta aatctcccac
    27721 ggtgtcttcg ggatgtctgg cgtagattca gcaacctggc gcaagagttc tgctttaagt
    27781 atctcgagat tatggatcgc agagtggtaa tcgcctgcca caaggatatc taacacaata
    27841 tcaatacgtt cggcaacgcg ctgggcatca aattcagaca tcaaggacat ccttatagca
    27901 aaagggggaa aatgaataat gcaaaaattg ccgctaaaag agaagtgttt aacagcaacg
    27961 gctaattatc atccaggaat acgatatata atgacgggat atagcgctaa gtatatatat
    28021 tcatctactt atgcgcgctt cagatagcgt ttataccagc gttcgaaggc gacggcgggc
    28081 atcggtttgg caaacaaaaa tccttgccgc tcattgatcc cgttcttggt taaaaaagca
    28141 tcttcctttg cactctctac accttcggcg atcacctgaa gattcaatgc ctgggccaca
    28201 gcgacgatcg cccggaccag tgactgcgag acaggttgtt tgtgaatatc tcgaacaaaa
    28261 acctggtcaa gtttgatggc atcgatcgga aagcgcgcca gttgcgaaag tgaagagtag
    28321 ccggtaccaa aatcatccag atgcacttgc gcacctagtt ggctaaattg ttgaataaca
    28381 gacagtgcca gttcatcatt ctcaatcaga caactctctg tcagttcaac atctataggg
    28441 cagtattcaa aattgagttc ctggagaacc tgtttcaggg cggtgaaaat ggtttgatcg
    28501 gcgagctgac gtgcagaaat atttaccgcc actcgcaggt ttatgccttt atcccgccac
    28561 tttgccacct ggcgtacgac atcgagaatc acccaacggc ctaaaggcac aattagccct
    28621 gactcttcgg cgtaggaaat gaagtccaac ggtggaatca acccacgttc aggtgactgc
    28681 caacgtacta gtgcttccag actgcgcact tcgacacgct aggtgatttt cgataaccaa
    28741 ctgatcgttt tccagtgctt tacgcaagtt ggtatccagc cagagatatt caaatacccg
    28801 ctgattcatt tctggagtaa aaacgcaaaa ttgtcctcgt ccgccttcct tcgctgtgta
    28861 cattgctgtg tcggcgtgac gaataatagt cgcgctgtcc gaaccatgtt cgggagagag
    28921 cgaaatacct actgaacagc tggtataaac ttcaattaaa ccaatacgaa aggggaggcg
    28981 taagcgggtc aaaattcgtg atgccattgc ttccagcgcg ctttgtgagg tattggatgc
    29041 cagtaccaga aactcatcgc cacctggacg cgccaacacc tggtcatgtt cgagacagct
    29101 taaaatagcc aatgacacgt cgcgtaataa ctggtcacca aacaaatgcc cataggcgtc
    29161 gttgaccttt ttaaaattat ccaaatcaag atacacaacc ccaactttat tgttatctgc
    29221 ctgattaata gcgtgatcga ttaactcctg cattgcgtta cgattcggca gtccggtgat
    29281 actgtcggta tttgccagaa tacgcagtcg ctcctgagtg cggcgctctt cggtaatgtc
    29341 ggtaccggaa cagattaaaa aaatctcgtt tttgccactg ccgctgtgga caaatttatt
    29401 gcgaaacaga aacagccgct ggcctttacg tgttggtatc cacagttcga cttcatatgc
    29461 attgccgctt cgaaaaaata cacggttatt gcgcctggat gccgcagctt cacgacggct
    29521 cataaacagt ttaaacacgc tttacccaat gacgtcgtgt tctttcaacc ctgtgtaatc
    29581 ttcacataac cgattgaagc gttggatatt cccccggctg tcgagaatca ctattgcgga
    29641 atgtgcttca gaaactacct gctcagcaaa ggaaagcccg tgtgataaat cacgagcaac
    29701 cgccggtgtg tcatgccatg cagacgcgtt gccagcccat tcccgtttat tgatttttcg
    29761 tcctaccaga tgcacagaaa gatcgctttc atcgacggtc agactcatca tcaggctgga
    29821 ggtgatgacc gtcatttccc tgatgcgagc agcctgttcg gcagataact gcaatgtacg
    29881 atcaggttct gtggttttgg tggtagaaaa gcgcaaagca tcgctgcttt ccgtcaaccg
    29941 ccagtatgga ttgtgcgatc cgagaaagtt gtacaacgtt gtggactccc taacggtttt
    30001 catgatgcat atcctccggt taacagcgga gtccgcggtt aaataaaagg aacaacatga
    30061 cttacatgaa attaacggcg gctaaacgca ttgttttttt atatatttat ttgtaatcca
    30121 gttttggaaa aacgccagtt ttcaaacgaa agtcagttaa aaaatctgcc tggatataac
    30181 gaaggtagag cggggaaata aacggcccat ccatgaggaa tgggccgtga aaggagatta
    30241 cgcgaccggg cgcgcaataa tgctgcgggt ttccatgcgg acttcggcaa tggtgacgtc
    30301 aataacgtca gttactttgt aaaccgtttc acctttaatt tgtacagtgc cgttttcctg
    30361 gctgcaaacc agttcatcgc gcacagcgtg taagaaaggt gccggaataa aggcgatagc
    30421 gccgttatca accaaacgca cacgcatgcc gccacggctg atatcgacaa tttccgccgc
    30481 gaaacgggtg ccggtcccgg ctttatcttt caggaagcgt gcgtataacc agtcgccaac
    30541 atcacgttct gccatccggt tgagacggcg acgttcggcc atttggacag tgatttcatc
    30601 ctgcggacgc gtcgcagttt cgcctttgat aaccgctttc agcagacggt ggttgatcat
    30661 gtcgccatat ttacggatcg gcgaagtcca ggtggcgtat gcttccagac cgaggccaaa
    30721 gtgaggaccg ggttcagagc taatttcagc aaatgactgg aagcgacgaa tgcggctgtc
    30781 gaggaaacca gttggttgcg catccagttc acgacgcagt ttgcagaaac cgtccagcgt
    30841 gagcacttct tcggcatcga catgcagacc gtgcgttttc agcaacgctg ccagcgcgtc
    30901 ggcattcgcc ggatcaaagc ccatatgcac gttatagatg ccaaaaccga gcttatcgcg
    30961 cagtacgcgg gccgcacaaa tgttagcggc aatcatcgct tcttcgacga tacggttggc
    31021 aatgcgacga ggctcggcga cgatatccag cacttcacct ttttcaccga gaataaagcg
    31081 gtaatccggg cgatctttaa acaccagtgc gtggttatga cgccactcgc cgcggcgttg
    31141 gcaaatttgc gctagcaaac ggacttgttc ggcaattgct tcactttcag gcttccagtc
    31201 accggtattt tccagccagt cagaaacctg gtcatacacc agcttcgctt tggattcgat
    31261 ggtggcggca aagaattcga tattatcttc aatggtgcca tcagcggaga gcgtcatgcg
    31321 gcatgccagt accgggcgga cttcattggc gcgcagtgag cagagatcgt cagaaagctc
    31381 gcgaggcagc atagggatgt tgaagccagg cagatagttg gtgaatgcgc gaattttcgc
    31441 ggctttgtcc agcttgctgc cttcagcaat ccacgcggtt ggatcggcaa tcgccacaat
    31501 cagctgaagt ttgtcatccg gcagcgcctt agcgaaaagg gcgtcatcca tatcttcagt
    31561 gctggcactg tcgatggtga caaaatccag cgaggtcaga tcttcacgaa ccagaccttc
    31621 atcgagcatt tcggtagcga cgccgtctgg tgcttctttt tccagattat ggcgcgcaag
    31681 ggtaacccac cacggtacaa agtggtcgtc accaaaagtg atgtattgtg tcagttcagc
    31741 atagaaagaa cgatcgcctt tcagcggatg acggcgcatt tcggcaaccg cccagtcgcc
    31801 ttctttaaac tcgtggttca ggccacgggc tgcgcggcaa ggaatggcgt cttttaaaag
    31861 tggatgatca ggaacgatgg tcagacggtc atttttgccc tgaactttac ccacgaaacg
    31921 agtcaggaac ggttcaacca gttcttctgg ctctgcggat tcacgttctt tttcactgtg
    31981 gatcaccgcg ataattcggt cgccatgcat gacttttttc atctgcggcg gcggaatgaa
    32041 ataacttttt tgtgcgtcga cttccaggaa gccaaagcct ttttctgtgg cttttaccac
    32101 cccttcagcg cgtggcgtct gggaatgcag ttgctgttta agctgcgcta gcagcgggtt
    32161 gtcctgaaac ataattgtct attttggtgg ccattagagc ggctgacagt tttacgcgaa
    32221 tctgtctgac gcggcaaggt taatatgtct cacccaacgc gatttttaag cgatttatcc
    32281 agccacacag cccgctccat accagcagat taataatctg cgttgatgat ttttcgtgtt
    32341 cgagtaaagg gctgaactgg gcggcgctaa agcgatcggg tgctcgggtc agccattgta
    32401 tcgcctgaac ggtagcgcgc tcaacagaat gacggtcagc ccatcggtta atttcatttt
    32461 cgcccttgcg cagaagggtg ctgatttttt tatattcatt tgatgaatcc atacaggtgg
    32521 aaaaacaagc aggactgcca tttatacgcg cgcataacaa cgccgcaagc tgattatccg
    32581 gctgtgcatt gacccgtgtg ctgtttaaca acttttcgag aagggaaagt gaaatagggt
    32641 ggagagagag tgtttcggcc agttggcaaa gttctgcagt tgaacttttt gtgtgctctt
    32701 caggtagttt ttccacctca tagctgcttc gccagcgtat tgattcatca gcaaacagtg
    32761 acgcgtcggc gtagttttgt atcgccagcc cgggtaacca gcgtaccgga tgcccgagat
    32821 aagcctgaaa tgtcgcaatt gtccgcgcct gaaagccaat aaaaccaatg atttggttaa
    32881 tgatcacaca gtcccagaca gtaagaccga cgtcattcag atgctggcgc gatcggttgt
    32941 cgataataga aggcgagcta gccagttggc gggcatattg cgtgatttgt gccaaccgat
    33001 gattactttc cctggaggaa tcaggtccgg ggagcggcgt aagtcgcgcc gaatagtagt
    33061 tacataaccg ctggacgccg caaacctgag caaccgtcag ggccgtcgac aagcgttcat
    33121 aagcggtgaa ggtttgtgaa cgggtcacgg taagctggtc agggaacaac tccactgcaa
    33181 gaacgcgcgc agggaccagc catgaaagga agggggccag cgtctcttca tcaaggatca
    33241 cgtcgagcag aaagggatcg ctgacctttg cggcttccgg gaccagaggc agaacgtcat
    33301 actccgtagt actggattgc gtttcatgat accagtggct tttgccggtg atgtggcgtt
    33361 gttccatggg cgctccttgg tcgtaaagga aatcgttatc ctgacgcaag gcgggaaggg
    33421 gagaaagacg gatcggcgat aacaaatatc agaaaggtat aacagagata acgggcggca
    33481 gaacgccgcc catctttacc aacagaacga ttatttcagt tcgagttcgt tcattgcagc
    33541 gatgctgaaa ccgccgtcaa cgtggactac ttcaccggag ataccggcag agagatcgga
    33601 gcacaggaat gccgcagagt tacccacatc ttcaatagta actgtacggc gaatcggggt
    33661 aacggcttcg caatgagcca gcattttgcg gaagtctttg ataccggagg ccgccagagt
    33721 acggatcgga ccagcagaga tggcgttaac acgcacacct tccggaccca tcgcgttcgc
    33781 catatagcgc acgttcgctt ccagagacgc ttttgccaga cccataacgt tgtagttcgg
    33841 gatagcgcgc tcagcgccaa ggtaggaaag ggtcagcagg gcagaacccg gattcagcat
    33901 ggagcggcaa gcttttgcca ttgcaacgaa gctgtaggag ctgatgtcgt gggcaatttt
    33961 gaagccttca cgggtaacgg cgttaacata gtcaccatcc agctgatcgc caggtgcaaa
    34021 accaatagag tgtacgaaac cgtcaaattt cggccaaact ttccccagtt cagcgaacat
    34081 ggtgtcgatg ctggtatctt ctgcaacatc gcactgcaga acgatgtcag aacccaattg
    34141 agcggcaaat tcttctacgc ggcctttcag tttgtcgttc tggtaggtga atgccagttc
    34201 agctccttcg cggtgcatcg cctgagcgat accgtaggcg atggacagtt tgctggcaac
    34261 accggttacc agaatgcgct taccggaaag aaaacccata gctttaatcc ttattgttga
    34321 tgcttgttgt gcctgaaaat caggcgattc gtcgttttag taaacagtac gaacagataa
    34381 acggttatta taatcaacct ggctgtgagt agctatagtt accaggtccg accggagcag
    34441 gctgcggcag ggggggcgct tttcccctta ccctaaccct ctccccagag gggcgagggg
    34501 accatattgt gcaaatattg ttaccccagc aacaaacagg ctcatacagc ccctaaccct
    34561 tttatggcga tggctgggat ggttcagacc ttgccgaata ttctccagca ctgtctccat
    34621 gttttcacca caacagctat tcggctcggt cggccccctc gccctttcag ggtgagggtg
    34681 accggcggtt cagttcctgc agaataatct ccagcaccgc ctcctcatta caatcaatct
    34741 cgttattcca gaaacgcagc acggtccagc cctgcgactc aagccagcta gtgcgcctgg
    34801 tatcataggc aactgccaaa tcatgctgcc caccatccag ctcaacgact acgcgcgccg
    34861 agcagcaagc aaaatcgaga atgtagctcc ccaccggatg ttgacggcga aatttgaaat
    34921 caccaaaacg tcggctgcga agatatcgcc agagctttcg ttcctgcaaa gtgagattgc
    34981 gtcttaaatc acgcgcattt gattttattt tatccatcac ctcattctga caagatttaa
    35041 tctcttgtca gaatgaggtg aataatctgg aaggaggatt cagaaaatta gcgatcttta
    35101 cgccacgcat ccgctgtcaa tgcttcgcca aagtgcccgg caatcagccg tttggtgagt
    35161 tcatgcagcg gcgatgccag cacatccgcg gtgctgcctc gctcgacaac ctcgccctga
    35221 tgcatcacca gcacctggtc gctaatgtgc ttcatcattc cgatatgctg ggtaacataa
    35281 atatacgaaa tgccctgttt ttcctgtaac tccagcatca gattaatcaa ctgcgaacgc
    35341 attgacatat ccagtgaagc gagggcttca tcggcaataa tgacttttgg gcgcaatatc
    35401 agcgcgcgcg ccagacccag acgctgtttt tgtccgggtg ccaacatatg cggatagtaa
    35461 ctgacgtgat ccggtagcag cccaaccata cgcatcgttt caataatctg tttgcgacgc
    35521 tgttccggtt ccaggtcggt gttcaggcgc agtggaaaat ccagaatttg tgagatacgt
    35581 tgacggggat tcaacgaggt cgaaggatcc tgaaaaatca tgcgaatacg ctgactacgg
    35641 aaggaataat cgccaaaatg cagcggatga tcgtcaatca ataactcgcc gctggtaggt
    35701 tctatcattc ccgccagcat ttttgccagt gtggatttac ccgaaccatt ctcgccaata
    35761 atcgccagtg tctggccttc acgtagcgta aagctcaagg gttttaccgc ttctacggtc
    35821 tgacgacgaa accaaccggt ccggtagcgg aacgttttac ttaaattacg cacttcaagc
    35881 agcgtttcga tcatctcact ctttctccat gttcagcggg aaatgacagg catagagatg
    35941 atttttcgcc cccgtcaaac gtggcgtcac aatgcattct cgttgtgcat acgggcaacg
    36001 tggccccaga cgacaaccaa tcggtaactg ttccagcaat gggatagcgc cgggtagagt
    36061 attgaggcga cttttatgcg gcatcgcgct gccgaagtct ggtatcgcgc ggatcagcgc
    36121 ctgggtataa ggatgatgtg gcatcgtcac caactcctta ctcggcgcgg tttccactgt
    36181 ttgaccgcag taaagcacgt taattttatc cgcccattgg ctaagcattt gtaagtcatg
    36241 gctgataagc aaaatagtgg tattgctgtt ttggttgaga cgcgtcagca ggcgaaagat
    36301 ttgcgcctgg gttgttggct ccattgagtt tgtcggttcg tcagcaatca gcagacgcgg
    36361 ttgattcgcc agtgcaatgg ctatcatcac tttctgacat tcaccttcgg tcaactcata
    36421 gggaaaactg cgcatcgcat ctttgtgatc tttaatcccc acgcggtgca gcagttcaat
    36481 ggcacggcgt ttgcgccagc caaaacgctg ccaccaacgg cctttatagg tccaggctgg
    36541 gatgttttgc atcaactggc gacccacacg ttctgaaggg tcaagacacg actgcggttc
    36601 ctggaaaatc atcgacacgt tatggccaac cagtttgcgc cgttcgcgtg cggagagacg
    36661 cagcaaatcg atatcatcaa aacgcatacg gtcagcagta acacgccagt tatctttatt
    36721 caccccacaa atcgctttcg caatcaaact cttgccggaa ccggattcac caacaagacc
    36781 gcggatttca ccttcggtta aggtcatact tacccggtcg acggctttaa cccactcatc
    36841 accggtttta aattcaatgg tcaggttacg aatatcgagt aatggcatta ttccaccccc
    36901 gcaataatcg cacgacgaac gccatcgcca aggaggttaa ccaacaacac gctaatcata
    36961 attgccgcac ctggcagcat gacagtccac ggggcgacat aaatcagttc cagcgcatca
    37021 ccgagcatcg ctccccattc aggcgagggg agttgtgcgc cgagatcgag aaagcccagc
    37081 gcggcgatat cgagaattgc catcgacagt gcgcgggtga tctcggtgac cagcccggcg
    37141 gtgatgtttg gcatcacagc aaaccagaga atattcagcg ttgatgcgcc atccagacgg
    37201 gcggcgataa cgtactcttt ttccagttcg tcatgcacca tgctgtaaat cgaacgtacc
    37261 atacgcggca gcaatgccag ccagacagca aacatagcgt gggacaaact cggtccggca
    37321 aacgctacaa cgataattgc caacagcagc gaagggatcg ccagtaaggt atccagaata
    37381 tggttaagca ccgctgagcg gaggccgtgt gtcgccccgg cgaaggtgcc cagtaccagg
    37441 ccacagatcg tcgcggcaag cgtcaccaca aatgcgccac ccacggttgg cgcagctccg
    37501 ctcagtaaac ggcttaaaac atcacgtccg aggtcgtcag tccccaggaa gaaagaaact
    37561 tcgccatagc gcgaccatga tggcggcaat aattgataac cgagaaattg ctggtcgatg
    37621 ccgtaaggcg caaaccagcc gccaaaaata cacagtacag ccagtcccgc gcagccgtac
    37681 aggccgacca tcgcagaggc gtcactataa aatttgcgcc aggcggtacg cagcgtgccc
    37741 ggcgggcgct tttcgctgta tacgctatcg taaggcatac cattccttat gtttcagagg
    37801 gttagccatg gcacccaaaa tatcagaaat cacgttaaca ataatgacca gtgagccaca
    37861 caccatcact ccggcggaaa tggctgcgta atcctgctgg cggatggcgt taattaacca
    37921 gcgccctaaa cccggccagc taaaaaccat ttcggtgatc atcgccagcg tcagcatggt
    37981 ggaaaactgt aagcccaggc gaggaataac cggcggtaac gcgttatgca gaacgtggcg
    38041 acgcaaaata gtaaagcgcg acaaaccgcg ggtcgccgcc gcttttacat agttctggtc
    38101 atacacttca atagtgctga tacgcatcag gcggatcact tcagttgttg gcgcaaccga
    38161 cagggtgatc acgggcaata tcatatggcg gattgcgctc atgatcattt catcccgcca
    38221 tggtgagtcc gaaagccagg catcaatcaa cgcaaaaccg gtaatcggtt tcacttcgta
    38281 gagcagatcg aaacgccctg aaacgggcaa ccaacctagc gtgagtgaac aaaacagtgt
    38341 caacagaagc gccagccaga acaccgggat tgaaaagccc agtaaggcga tggcgttgat
    38401 caagttatcc tgccacttat ggcgcgtaat cccggcaatc atccccacgg ggatgccaac
    38461 aatcagcgca aaaccgaagg cgaggatgca cagttctatc gtcgccggga agagctcttt
    38521 taactgttcg gcaattggtt gaccgttaat gctggaaaca ccaaaatccc agtggatcag
    38581 gccattaaac cagaacaccc aggcattcca cagtgacgcg ccttgtaacg gcgcgtgtgg
    38641 agtgaaatag ctcaagctga agccaacgaa ggtcagcagg aacaaggtga caatcaataa
    38701 caaaatgcgg cgtaaggtga agataatcat ggttttttca cctcatcctg tttctcgcga
    38761 tacaccccag caaaggaggc gttaccaaac gggctaagta ccagaccttt gatatcgtac
    38821 cgataggcct gcaaacgcaa tgacgacgcc agcggcagaa taggtaattc ctgcgccaga
    38881 atactctgcg cttcgtcata ggcttcaata cgcgctgcca gctgctgcga ggagagcgcc
    38941 ttacgcaaca cgctgtcgaa tttcggattg caccagtggg cgaggttggt ctgtgaatga
    39001 attgccgcgc agctcagtaa cggacggaag aaactgtccg ggtcgttact gtccgtcgcc
    39061 caaccggata acgtcagatc atggctcata tccatcaacc gcgcctcctg aaagcgacct
    39121 tctaccggca caatcaccac ttttacgcca acctgcgcca tatccgcctg aatcagttcg
    39181 gcagttttca gtggactggg gttccacgcc tgcgaacgtg tgggcaccca cagtttcagc
    39241 gttaagtttt ccagccccaa cgccttcaac tgttcgcgcg atttcgccgg attgtattca
    39301 gtaattttag cctcgttgtc ataggcccac gaggcgcgcg gtaaaataga ggccgccgtt
    39361 tcagccgtac cataatagat ggattgcatc aggcgctggt tattaatcgc cagtgccagt
    39421 gcatggcgga cagcgggatt atttagcggc ggtttggcgg tgttaaatgc cagataggcg
    39481 acgttcatcc caggacgcag cgttaaacgc aagcgcgggt cgtcacgcaa aatggatagc
    39541 tggctggcag caggccaggc cagaacgtcg cattccccgg tcaggagttt cgacagacgt
    39601 ccggtgccgc cggagcctaa atccaccact acctgcggca ttaacggttt accgcgccag
    39661 aagtcatcat gacgttgtag gcgaataaat tgcccggcgc ggtattccga caactgatac
    39721 ggaccggtgc cgaccggttg acggtcgagt tgctcctggc gatcttcttt ctctaacttc
    39781 cgggcatatt ctgccgacat gaccgaagca taatgggttg cgaggtgcca caaaaaagaa
    39841 gcatccggct gggcgagtcg gaactcaacg gtatgattat ccagtttgcg gacgcttttc
    39901 acgttatcgg caaattgcag gctgtcgaag taggggaagt tgctgccgtt gacgttatgc
    39961 cacgggttgt tgcggtcaaa aattcgctgg aaggtaaaca ccacatcgtc ggcattcatt
    40021 ttacgagtgg gagtaaacca gtcggttttt tgaaacggaa catcgcgacg caggtggaag
    40081 cgataggtcg cgccgttgtc gagtacttcc cagctttcgg caagttccgg catcaggcga
    40141 taggtatagg gatcgacatc cagcagtcga tcataaaact gggcggcaag ggtatcgaca
    40201 attaacccac tgctcgcttt ggatgggtta aaggtg
//
LOCUS       DAJTYL010000023        39728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYL010000023 DAJTYL010000000
VERSION     DAJTYL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.023"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1077
                     /gene="cscA"
                     /locus_tag="OO572_001199"
     CDS             <1..1077
                     /gene="cscA"
                     /locus_tag="OO572_001199"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6660274.1"
                     /translation="DGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPK
                     VWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPDFFS
                     LGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAP
                     QSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESL
                     RQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSE
                     RLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPE
                     ERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1085..2143)
                     /locus_tag="OO572_001200"
     CDS             complement(1085..2143)
                     /locus_tag="OO572_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6660275.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2311..2529
                     /locus_tag="OO572_001201"
                     /pseudo
     CDS             2311..2529
                     /locus_tag="OO572_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2547..3875
                     /gene="dsdA"
                     /locus_tag="OO572_001202"
     CDS             2547..3875
                     /gene="dsdA"
                     /locus_tag="OO572_001202"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6660276.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(3983..5521)
                     /gene="emrY"
                     /locus_tag="OO572_001203"
     CDS             complement(3983..5521)
                     /gene="emrY"
                     /locus_tag="OO572_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6660277.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5521..6684)
                     /gene="emrK"
                     /locus_tag="OO572_001204"
     CDS             complement(5521..6684)
                     /gene="emrK"
                     /locus_tag="OO572_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6660278.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7100..7714
                     /gene="evgA"
                     /locus_tag="OO572_001205"
     CDS             7100..7714
                     /gene="evgA"
                     /locus_tag="OO572_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6660279.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7719..11312
                     /gene="evgS"
                     /locus_tag="OO572_001206"
     CDS             7719..11312
                     /gene="evgS"
                     /locus_tag="OO572_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6660280.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11368..12513)
                     /gene="yfdE"
                     /locus_tag="OO572_001207"
     CDS             complement(11368..12513)
                     /gene="yfdE"
                     /locus_tag="OO572_001207"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6660281.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12587..13531)
                     /gene="yfdV"
                     /locus_tag="OO572_001208"
     CDS             complement(12587..13531)
                     /gene="yfdV"
                     /locus_tag="OO572_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6660282.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13601..15295)
                     /gene="oxc"
                     /locus_tag="OO572_001209"
     CDS             complement(13601..15295)
                     /gene="oxc"
                     /locus_tag="OO572_001209"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6660283.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15349..16599)
                     /gene="frc"
                     /locus_tag="OO572_001210"
     CDS             complement(15349..16599)
                     /gene="frc"
                     /locus_tag="OO572_001210"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6660284.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17112..17735)
                     /locus_tag="OO572_001211"
     CDS             complement(17112..17735)
                     /locus_tag="OO572_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6660285.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18032..18307
                     /locus_tag="OO572_001212"
     CDS             18032..18307
                     /locus_tag="OO572_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660286.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18384..18626)
                     /locus_tag="OO572_001213"
     CDS             complement(18384..18626)
                     /locus_tag="OO572_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6660287.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            18979..19899
                     /gene="lpxP"
                     /locus_tag="OO572_001214"
     CDS             18979..19899
                     /gene="lpxP"
                     /locus_tag="OO572_001214"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6660288.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20254..20325
                     /gene="ypdK"
                     /locus_tag="OO572_001215"
     CDS             20254..20325
                     /gene="ypdK"
                     /locus_tag="OO572_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6660289.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20390..21628)
                     /gene="alaC"
                     /locus_tag="OO572_001216"
     CDS             complement(20390..21628)
                     /gene="alaC"
                     /locus_tag="OO572_001216"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6660290.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22005..23491
                     /locus_tag="OO572_001217"
                     /pseudo
     CDS             22005..23491
                     /locus_tag="OO572_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23506..24240
                     /gene="ypdB"
                     /locus_tag="OO572_001218"
     CDS             23506..24240
                     /gene="ypdB"
                     /locus_tag="OO572_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6660291.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24253..25110
                     /locus_tag="OO572_001219"
                     /pseudo
     CDS             24253..25110
                     /locus_tag="OO572_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25113..27608)
                     /gene="ptsP"
                     /locus_tag="OO572_001220"
     CDS             complement(25113..27608)
                     /gene="ptsP"
                     /locus_tag="OO572_001220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6660292.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27633..28670)
                     /gene="ypdE"
                     /locus_tag="OO572_001221"
     CDS             complement(27633..28670)
                     /gene="ypdE"
                     /locus_tag="OO572_001221"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6660293.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28670..29753)
                     /gene="ypdF"
                     /locus_tag="OO572_001222"
                     /pseudo
     CDS             complement(28670..29753)
                     /gene="ypdF"
                     /locus_tag="OO572_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29768..31015)
                     /locus_tag="OO572_001223"
     CDS             complement(29768..31015)
                     /locus_tag="OO572_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6660294.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31037..31363)
                     /locus_tag="OO572_001224"
     CDS             complement(31037..31363)
                     /locus_tag="OO572_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6660295.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31582..32547)
                     /gene="glk"
                     /locus_tag="OO572_001225"
     CDS             complement(31582..32547)
                     /gene="glk"
                     /locus_tag="OO572_001225"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6660296.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            32751..34007
                     /locus_tag="OO572_001226"
     CDS             32751..34007
                     /locus_tag="OO572_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6660297.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34122..34448
                     /locus_tag="OO572_001227"
     CDS             34122..34448
                     /locus_tag="OO572_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6660298.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34589..35827)
                     /locus_tag="OO572_001228"
     CDS             complement(34589..35827)
                     /locus_tag="OO572_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6660299.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36163..37365
                     /gene="nupC"
                     /locus_tag="OO572_001229"
     CDS             36163..37365
                     /gene="nupC"
                     /locus_tag="OO572_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6660300.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37415..39527)
                     /locus_tag="OO572_001230"
                     /pseudo
     CDS             complement(37415..39527)
                     /locus_tag="OO572_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10589 a         9886 c         9190 g        10063 t
ORIGIN      
        1 gatggtgcag gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat
       61 ggtattcatt tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc
      121 cgcgatccta aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat
      181 ccaggcaaca cggggcagat cctgctttat cgcggcagtt cattgcgtga atgggccttc
      241 gatcgcgtac tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt
      301 ttcagccttg gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga
      361 tacagttacc gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga
      421 ctttttgcac aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca
      481 caaagctttt tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa
      541 tcgccaatgc cctcaaaacg tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta
      601 tcagagagca atggcaaact tctacaacgc ccggttcacg aagctgagtc gttacgccag
      661 cagcatcaat ctgtctctcc ccgcagaatc agcaataaat atgttttgca ggaaaacgcg
      721 caagcagttg agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga
      781 ttacagctcg gcactggaat gcggctgtat attgataacc aatctgagcg acttattttg
      841 tggcggtatt acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt
      901 gacacgctcg ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac
      961 ggggaagcgg tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt
     1021 tatgcctccc acggagtggc tgtgctgcaa catggagcac tctggctact gggttaacat
     1081 aatatcaggt ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt
     1141 cacgaccttc aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa
     1201 ttgtggtcag cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta
     1261 cagcgacatc ctgcgggata cgtaccccct tcgccaaaag aacctgataa gccacaaagg
     1321 ctgcgcgatc gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtgag
     1381 cattgagtaa acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt
     1441 tcacctcagc cagatctcgt ccagcatcac gccaagcctg ctcaaatccc tgccgacgat
     1501 accctgttgc caacgcactt tccggtagcc agaagcataa tggttgacga tagcccgccg
     1561 cgagcaaatg ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta
     1621 acgctgggtc atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag
     1681 gcagcgtgat atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa
     1741 gcagctgacg tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat
     1801 tccagccgaa ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag
     1861 tggtagccgt gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca
     1921 tcttacgggc ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt
     1981 cacgcgttgc aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg
     2041 acactcccgc caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa
     2101 aacgggcact gtagcagaaa cagacgtcac tggcgagatc caacgcccta tcacctgaca
     2161 cagcaataca ataaaaaata acaataattc ccggacaatt gtccccaatt ccgcctctgt
     2221 tctcgccaac gagtaaccat ataggcaagt aagtaaaaca tgccaactgg catcgccaac
     2281 ctggagaaga aaatataaat atggatcatt gtgctgccac tgtatcccga catcagcccg
     2341 gaaattattg cgattgctat cggttcaggt gcaattggct gcacgatcgt tacggactcg
     2401 cttttctggc tggtgaagca atattgcggc gctacgctca atgaaacatt taaatactat
     2461 acgacagcga catttatcgc ttcagtcgtc gctctggcgg gcacattcct gctgtcattt
     2521 atcatctaag cgcaaagaga cgtactatgg aaaacgctaa aatgaactcg ctcatcgccc
     2581 agtatccgtt ggtaaaggat ctggttgctc ttaaagaaac cacctggttt aatcctagca
     2641 cgacctcatt ggctgaaggt ttaccttatg ttggcctgac cgaacaggat gttcaggacg
     2701 cccatgcgcg cttatcccgt tttgcaccct atctggcaaa agcatttgct gaaacagctg
     2761 ccactggggg gattattgaa tcagaactgg ttgccattcc ggctatgcaa aaacggctgg
     2821 aaaaggaata tcaacaaccg atcagcgggc aactcctact gaaaaaagat agccatttgc
     2881 ccatttccgg ctctataaaa gcacgcggcg ggatttatga agtcctggca catgcagaaa
     2941 aactggctct ggaagcgggg ttgctgacgc ttgatgatga ctacagcaaa ctgctttctc
     3001 cggagtttaa acagttcttt agccaataca gcattgctgt gggctcaacc ggaaatctgg
     3061 ggttatcaat cggcattatg agcgcccgca ttggctttaa ggtgacagtt catatgtctg
     3121 ctgatgcccg ggcatggaaa aaagcgaaac tgcgcagcca tggcgttacg gtcgtggaat
     3181 atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa agcagcgcag tctgacccga
     3241 actgtttctt tattgatgac gaaaattccc gcacgttgtt ccttgggtat tccgttgctg
     3301 gccagcgtct taaagcgcaa tttgcccagc aaggccgtat cgtcgatgct gataaccctc
     3361 tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcgggctta
     3421 aactggcgtt tggcgatcat gttcactgct tttttgccga accaacgcac tccccttgta
     3481 tgttgttagg cgtccataca ggattacacg atcagatttc tgttcaggat attggtatcg
     3541 acaaccttac cgcagcggat ggccttgcag ttggtcgcgc atcatgcttt gtcgggcggg
     3601 caatggagcg tctgctggat ggcttctata cccttagcga tcaaaccatg tatgacatgc
     3661 ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc ttcagcactg gcgggtatgg
     3721 ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag
     3781 aacaattgcg taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag
     3841 aagagatgaa tcaatatctg gcaaaaggcc gttaataacg tttcaacgca acatcgcaat
     3901 cctttccctg ggtgagcgat gctgccgatg gcgcagactt aagatccccg gtcttacccg
     3961 ctataacccc ctccttttat catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa
     4021 accaaaccaa aaccgtaagt aagataaaag caatagccgc catacggaaa atctcatttg
     4081 cagaaataga aagtgactgc tgggttattt cattattaat ttcattaaga actcctgaaa
     4141 gcgaaccata gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat
     4201 cgatggttgc tgtcaactga ctatggtgta acgattcgcg tcgtccccac agcgtcattg
     4261 tcaacgacgt accaactgat cctgacaagg tacgaaaaaa attactcata ctcgaggcat
     4321 tggcaaattt attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac
     4381 aggcaacggc gaatccctgg aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg
     4441 gcataaatgt cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctgaatgtca
     4501 ctaacacccg catgtctatt ttattgccat aacgtcctat caaaggtgaa atcaatagtg
     4561 gcatgatgcc gatgggcgca taagcaagcc cggcccatat cgcattatac cccatcgttt
     4621 tctggagtaa ctgcggcata aggacgatcg ctccagagta aaataaatac gcgcaggtga
     4681 tactcacaat accaatggtg aaattacggg acttaaacaa actgagatca agaatcgggt
     4741 tctctgaggt cgattcccaa atgactaaag agatcagaga aataactgat actactgtta
     4801 atattattat tgtactcgag ttgaaccaat ccagatcgcg ccctttatca agcataattt
     4861 gcaagccacc aacaccgagc actaacaggg tcagtcttgg tagattcatt tttaccggtg
     4921 aagtttcagt ttctcttcct ttaagtaagg ttaagcataa tgtcaggacg ataatcccca
     4981 tagggacatt gattaaaaat atccaacccc agctaaagtt atcgcaaata tagccgccca
     5041 atatcggccc acatatcgga gcgataatca cggtcattga ccataatgcc agagcaaatg
     5101 ttcttttttc tggtggataa ttccttaata ataaactctg tgacagtgga attaacggcc
     5161 ccgccattaa tccctgaacg actctaaaaa atatcagcac atcaagattg gtcgataaac
     5221 tacacattaa ggaagacagc gaaaaaaaag tgactgaaag taaaaataac cttaattcgc
     5281 ctattctttg tgccagcctg ccggtaacag ggatcgcaat ggcatttgct acgccaaacg
     5341 aggtgataac ccaggtgcct tcgtctgttg atgctcccag aaagccagat attgtcggta
     5401 ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa cgtcgctaat gacaatgcaa
     5461 tagtaacgca ccataacgtc ccaccggtta atggtgccgg agttgattta gtgattgcca
     5521 ttaaagttgt ccattatgcg aaataatgtt gctaatttct ttttctatcg gactggtatc
     5581 gataactaaa gccttactgg tataagcagg catggaggtc acggttgaag ccagatcagg
     5641 catctcagca atgtcttcgt ccttcgtatc aatagttgca gtcatcgata aaccaatgcg
     5701 caaggggtgt tccatgagtt cttttggatc aagagaaact tcaaccggta cacgctgaac
     5761 gattttgatc cagttccctg tcgcattttg tgcaggtaat aaggagaacg cattgccggt
     5821 tcccatattg atccctgtca cccgaccatg aaacacaaca ttttcaccat aaagatcgct
     5881 gataatattg actgattgac caatccgtac atccgtgagt tgtgtttctt taaagttggc
     5941 attaacccac atttgacgtg ccggtacgac agccattaac gattgtccgg aactcactgt
     6001 ttcgccgacc tgaacacttc gctgggcaat atagccagta accggactcc ttatatccgt
     6061 acgtttaagc accaaccagg cttctttggt tgcatccgcc gcttcaacta cttgtggctg
     6121 acggtttaat ggtgtgttca ttactaaagc tttattcgct ttataagcct ggatagcggc
     6181 attcagtgcc gctttgctac ttattaacgt atctttggta tgctccagcg tttcttttga
     6241 aataaccccc tgcttcgcta aaggcacacg acggttataa tcttctaaag attgttgata
     6301 ctgaatacgt gctgaagcga cttcggcact gtattgttta tcctgtaagt acagtttatt
     6361 cgtttgccga acaatatttg ccagattatt tttagcttta ttgagtgcga tagtggcatc
     6421 agttttatcc agtgaaacta aaatgtcacc ctgtcgaacg tagttcgtat ctttatgatt
     6481 aactacagtg acactacctg agacttgtgc agaaattgga tctgcattcc ccgtgacata
     6541 ggcgtcatct gtactaatca tgtcttctaa ttccattgac caataggcat aggcacctga
     6601 aaacgcaata aataaaacta tcaccaataa agaaaagtat ttttttctgt tagactgttt
     6661 tttatttgaa ttaatctgtt ccactattat ctctcatttc gcatagatga aatttatgaa
     6721 ttgaatgaaa agagtattca ttcttcatat caaacacagt gcatgtgttg cacattatcc
     6781 caggaacgtt ggaggcctaa tttaaatgag gtgcaaaaaa aacaacagag gactaaaccg
     6841 tggcttttgc aatacaattc ttacgcctgt aggattagtg agaagactta tagtgctaac
     6901 ttgaaactat aaatcatcgg tataatccct gattttattg ttgacatttc atttatgccg
     6961 actatttata tggtatactt gtcgaattat cttaaaggaa actcagattt tcttattttt
     7021 attgagaaaa tgagatgacg ccttatgtct gtattactac agggagaagg gagatgcttc
     7081 attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc ttgctatcgc
     7141 agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcggagt taactgaagg
     7201 cggaagcgcc gttcagcggg tggaaacact taagcctgat atcgtcatca ttgatgtcga
     7261 tatccccgga gttaacggta tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg
     7321 aattattatt atcgtctccg ctaaaaatga ccatttttac gggaaacatt gtgctgatgc
     7381 tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac aatatcattg cggctattga
     7441 agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg ttggaagttt
     7501 aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg tcatgcggta
     7561 tattcttgat ggcaaggata ataatgacat tgcggaaaaa atgttcatca gcaacaaaac
     7621 tgtcagcacg tataaaagtc gcctgatgga aaaattagaa tgtaaatcac tgatggatct
     7681 ttacacattc gcgcaacgta acaaaatcgg ctaatcacat gaagttttta ccctattttt
     7741 ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacgaagat tacatcgaat
     7801 atcgtggcat cagtagtaac aaccgtgtca cacttgatcc agtacgtctg agcaacaagg
     7861 aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg
     7921 ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg tattaatgct gattatttaa
     7981 atcttttaaa aagagcgtta aatatcaaat taacactccg ggaatacgca gatcatcaaa
     8041 aagcaatgga cgcgcttgca gaaggtgaag tcgatatagt gttatcacat ttagttactt
     8101 cgccgcctct taataatgac attgctgcaa ccaaaccatt gataattacc tttccggcgc
     8161 tggtaaccac ccttcacgac tcaatgcgac cgcttacctc accaaaacca gtaaatattg
     8221 ctcgggtagc aaattacccc ccagacgagg taattcatca atcatttcca aaagcaacaa
     8281 ttatctcttt tacaaattta tatcaggcat tagcatccgt ctcagctggg cacaatgatt
     8341 actttattgg tagtaacatc attaccagca gtatgatttc ccgctatttc actcactcct
     8401 taaatgtagt gaaatattat aactcgccgc gtcaatataa ttttttcttg accagaaaag
     8461 aatctgtcat tcttaatgaa gtactcaata gatttgttga tgctttaaca aatgaagttc
     8521 gctatgaagt atcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat
     8581 tagaactcac tgaacatgaa aaacagtgga ttaagcagca tcccaattta aaggtgctgg
     8641 aaaatcctta ctcgcccccc tattctatga cggatgaaaa tggctcggtt cggggcgtta
     8701 tgggggacat tcttaatatt attaccttgc aaacaggttt aaatttttct ccgatcaccg
     8761 tttcacacaa tatccatgct ggaacacaac ttagccccgg aggatgggat ataatacctg
     8821 gcgctattta tagtgaagat cgagaaaata atgttttatt tgctgaagcc ttcataacaa
     8881 cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa
     8941 tgaaagttgc cattccatat tattatgagc tgcattcgca attaaaagag atgtatccgg
     9001 aggttgaatg gatacaggtc gataatgcca gcgctgcatt tcacaagatt aaggaaggtg
     9061 aacttgatgc tctggtcgcg acacagctaa attcgcgtta catgatcgat cattactatc
     9121 ctaatgaact ttatcatttt cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt
     9181 ttcctcgcgg agaaccggaa cttaaggata ttattaataa agcactgaat gcaattcccc
     9241 caagcgaagt tctgcgcctg acggaaaaat ggattaaaat gcccaatgtg accattgaca
     9301 catgggacct atatagcgag caattttata ttgttacgac attatccgtt ttattagttg
     9361 gcagtagcct tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc
     9421 agggtgattt agaaaaccaa atatcattcc gaaaagcact ctcggattcc ttaccgaatc
     9481 caacttatgt tgtaaactgg caaggtaatg tcattagtca taatagtgct tttgaacatt
     9541 atttcactgc ggattactac aaaaatgcaa tgttaccatt agaaaacagt gattcaccct
     9601 ttaaagatgt tttttctaat gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa
     9661 tatacacaca ggtatttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc
     9721 atacattatg caatcttcct gcaagtgaca atgcagtata tatttgtggt tggcaagata
     9781 ttactgaaac gcgtgatcta attaatgcac tcgaggtaga aaaaaataaa gcgataaagg
     9841 ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca cgaaataaga acaccaataa
     9901 gctctattat gggcttcctg gaacttctgt cgggttctgg tcttagcaag gagcaacggg
     9961 tggaggcgat ttcacttgcc tacgccaccg gacaatcact cctcggctta attggtgaaa
    10021 tccttgatgt cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata
    10081 tccctacttt agtccagaac acttgtcact ctttcggtgc gattgctgca agcaaatcga
    10141 tcgcattaag ttgcagcagt acatttcctg atcattacct ggttaagatc gaccctcagg
    10201 cgtttaagca ggtcttatca aatttactga gtaatgctct caaatttacc accgaggggg
    10261 cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa ccacgctgtt atcaaaatga
    10321 cgattatgga ttctggaagt ggattatcgc aggaagaaca acaacaactg tttaagcgct
    10381 acagccaaac aagtgcaggt cgtcagcaaa caggttctgg tttaggctta atgatctgca
    10441 aagaattaat taaaaatatg cagggcgatt tatcattaga aagtcatcca ggcataggaa
    10501 caacatttac gatcacaatc ccggtagaaa ttagccagca agtggcgact gtcgaggcaa
    10561 aagcagaaca acccatcaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc
    10621 cgaccaacag gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag
    10681 ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca acattatgat ctgcttatta
    10741 ctgacgttaa tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa
    10801 attcttcctt acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag
    10861 ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa
    10921 cacatttaag tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata
    10981 tcgaagccct gaaaaataat acggcgaatg atctacaact gatgcaggag attctcatga
    11041 ctttccagca tgaaacgcat aaagatctac ccgctgcgtt tcatgcacta gaagctggcg
    11101 ataacagaac tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt
    11161 tgcaaaagtt gattaatatt agccatcagt tagaaataac acctgcttca gatgacagta
    11221 agcctgaaat tcttcagctg ctgaactctg ttaaagaaca cattgcagag ctggaccagg
    11281 agattgctgt tttctgtcag aaaaatgact aaattgcggc tcccacaatg ttctaatgtg
    11341 ggagctattt accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc
    11401 atgctggtcg agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt
    11461 tatcggatta cccggcatca ttattcctcc ggcttcaatc aacatatttc tcgcctgagt
    11521 ttgtggcaaa ttaatggcct cagccacact taataacggc gcgacgggca caccaacttc
    11581 atgtattttg gctaaccaaa cttcagctgc ctgcgttttt aacgttcgct caatatattg
    11641 tttaagaata gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg gatcattaac
    11701 cagttccgta agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca
    11761 aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg
    11821 atttcccagg cgttgtggtg acttccctgt cgcaatatat gccatcagac catgctccag
    11881 aaaactcagc gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc
    11941 gcggccataa agtgcactca ctattccgct gaataaatag acaccgccgc atagatccgc
    12001 aagagaggta ccaacacgca ctggcggagc atcagggtac cctgtttcca tcattatccc
    12061 actcattgcc tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc
    12121 gaaacctgac gatgaagcat atatgaggcg cgggttgatt tcttgtaacc tttcccatga
    12181 aaaccccagt ttttccattg tacctaggcg aaaattctca gctaatacat cagcttgttt
    12241 gagcatattt ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc
    12301 tttgccatga ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt
    12361 gcgggtatca tcaccatgac ctggtggctc aactttaatt acccttgcgc ccatattata
    12421 aagaagttga gttccgaaag gtccattaag gacatgtgtc atatcgataa ctaataagcc
    12481 ttcaaacggc cctttgcttt cattatttgt catttgctta tgccttgaaa aaagccgaag
    12541 gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gattaattat gaaaccagtc
    12601 gactgacata aatccacaag ggagccgtga cgacaaagcc cagtacgctc acagccaatg
    12661 acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg
    12721 ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg ttcgctgttc aaatgacatg
    12781 ccataccaac gagaagcagc gccagtggca tcagaatcaa cttcaggaag gtgttataag
    12841 caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg
    12901 ctacccctga gttagccttc gcaatcagat taaaggttgg gtcccatgct gccggaattt
    12961 ttaccccaac cagcaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg
    13021 ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat
    13081 tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta
    13141 ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga
    13201 atccaatggt aggtgaaccc gcaattaatg cacatacagc tgcttctgca tgggtacgtt
    13261 taaaaaattt gtagcaacca aaccaggaga aaaagaaaca tccgacaata accacaagtg
    13321 atacaagggt cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa
    13381 caaatagagc cgcaggaaga gcgtagttta ataccagttt attaaatgcg cgggcttgat
    13441 cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag
    13501 gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac
    13561 catcgcataa tgagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt
    13621 ttgggttaag tttggtaata tggccacttt cagttcctgc tgcagggtcg ataaccacat
    13681 taataatggt cggtttgcgc gactgaatac cggtggttaa agcatgacga agttcatctg
    13741 tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat
    13801 ggtgcaacag atccgttggt gatggtgcac cagcgccact gagatcaaca ccgtctcctc
    13861 tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg
    13921 tttcaatttc catcccactg aaaccaaaag cactatcacc ttcaatggcg acaaccggag
    13981 aaccagaagt cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg
    14041 taccacaatc cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca
    14101 gggtatttgc accttcatta actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca
    14161 cagcacttaa tgcattaaag taatttaatg gttgagtatc tgtacttaat ttttcatgca
    14221 ttttttgtgc attttgctgc ttgtggatat ttaaaatatc gcgccatacc agtggagtcg
    14281 taaatgtgtt ttgtttcagt tctgccagca taccttgcat actggatgca atatcaccaa
    14341 cgactggcac agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa
    14401 taaactgtgt atctgccgcc catccttttt taccgtgtgc caataaccaa ttcagtcgtg
    14461 caccaacaag catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag
    14521 aaagtggatg cgtatcttca aggatccctt tcgccataga cattggcagg aatggaatct
    14581 gagcactttc aataaattca cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc
    14641 caaggataat taatggccgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact
    14701 tcgggcatgg caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt
    14761 cgtctttttc catcgtcgcg gccaggacat ttgctggcaa atcaagataa actccgccag
    14821 ggcgacccga tacagagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct
    14881 gattaacgcg aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt
    14941 cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta atcatgataa
    15001 tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa accattgagg aatcctggcg
    15061 cagaaactgt caggcagatc cccggttttt gggtaagaaa accgcttgcc gcagcggcat
    15121 agcctgccga ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc
    15181 gtgccatatc cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt
    15241 ttaatgcttc aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta
    15301 ataacctcga aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg
    15361 gttttgcttc attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc
    15421 ggtatgttca cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat
    15481 tggacaacca acggtcagat attttccacg caacggttgt tccacttcaa caacactgcc
    15541 actttggcgc aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcacaagg
    15601 aatatcaaac tgagtcaaat aggccaccgc ttcatgttta tcaatagtga cagtgtattt
    15661 ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc
    15721 cggatcggta atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc
    15781 ctgaatagtg aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat
    15841 ccagccaggc tgaccgccac cacctgcatt accaccgcgg ggaactgcat caccaaatgt
    15901 accattcgga tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc
    15961 acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc attgacatgg tgactcgttg
    16021 cccacgcccc gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat
    16081 tccggtgttg ctgtcaccca acgctgcagc gcttaccagc ggcggaccat cccaaaaacc
    16141 cgtagtggat gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac
    16201 ataaggcgaa cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc
    16261 ttgaatatgc tcccaggtga agcccatgtg atcaatggcc cctggatgaa agttctcgac
    16321 taagatatca gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt
    16381 atttaactca atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc
    16441 aggaatatct cgcagttggt gacgcgttac gtcaccaacg ccgggacgtt caattttaat
    16501 aacgtcagcg ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt
    16561 gaaatcgaga actttaattc cttgaagtgg agttgacata gcaataccta ttagttaatt
    16621 aaatatgtgt taatttagcg tttatttaag gcataacgcc ctctataaat aatctgcaaa
    16681 atatcttcgt agcggcactc gatattttta aattcctgta tcaagtactg aaaattattt
    16741 tatcgccact tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga
    16801 taaatattaa ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac
    16861 tgtaagaagc gccggtgatt tacaagtaag agtatgatta cgctttttaa agcgtaccat
    16921 aaagcataac ataataaatg atttgtgatg acacgggatt attgcggttt actttgagga
    16981 aaataaaatg cgtgtttata gaaccgcgag cgccaaataa ttttatgacc acaacctgtc
    17041 gtattgtggt cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact
    17101 gcctgtcagc attagttcac aaacagtgct tcgctatcga cgatgattcc atcttccgca
    17161 cctttaagcg ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca
    17221 gcaagtgcat tacgagtctg tttaagcggc atcagatatt ggttttccat tacgccaaca
    17281 ccggccaggc gaagctcttc cattgcgccc tttttatccc ctttcgccat tttttcattg
    17341 gcaattttta tcgcggcttc tttttcgggt gttgctacat aactctcaga aattccgaca
    17401 gatgcattaa tgacaatata ttggtcatca ttgagattgg tctttttacc tggcttagcg
    17461 aactttgccc attcggtact gtcatctgac agcaaagcgg aagcttcatt ggtcagttct
    17521 tttgcctttt ccggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc
    17581 gcatataaac cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgagtt
    17641 tgctgttgtg ccgctaccgg tgcattatca gcagcccata cagttgaaga tgccagaatt
    17701 gctgtgacca tcgtagccat aattaaacgt ttcatactta cctcattctt taacagtttt
    17761 agaaaacgcc gccatcatat tgggtgagca tgaaatgaga aatcagagag caacggtcat
    17821 tttgaaaggg ttatcttaca attgtagtta ttatcgcaaa gcggcattta gtcttacagt
    17881 tgtaggaata tgcctcctgt cgcagagaac tattcttata taccacaaag agatgtcatt
    17941 tattatattg atgtaaatat tgattaatta tatgtaaata cagccataac atcgtatggt
    18001 tctcagtgaa ccattttcag gataactttc tatgaaagta aacttaatac ttttcagctt
    18061 atttttattg gtctctatta tggcatgcaa tgtttttgca ttttccattt ctggtggtgt
    18121 aagtgagagg agctataaag agactgaaaa aacatcagcg atgacgacca cacactctac
    18181 aaaacttcag ccatcacagg cgattttgtt taagatgaga gaagatgtgc caccattaaa
    18241 cctcacagaa gaaatcacgc ctacttatcc gacaaaggcg aattatctta ttcatcctgt
    18301 gcgatagcgt ctgtcacatg gcaggtgaac aatcatgtct gatgctgatg tgaacacctg
    18361 cccaaatgag ccatcctgac ccactatcgc ctgccatacc ccaccataaa gcgaatcatc
    18421 aagccacaag ccggggcaag aaacataaat atccccgtca ccagttcatt accgccagtt
    18481 aaccacatgg tgaagtaata aatgagtgca gcgaggatca ccatgccgaa gaaagaagag
    18541 accgccgata ccacgttcag tacctgaccg atgtagccgc ttacgcagac aatacacagg
    18601 gcgagaaaca tccataaata aatcatagct caacctcctg ccgcgctaaa aaacctgtaa
    18661 cttcagtcac tttaatagta agacaatcct taacctctgg caatttttgc taagcatcaa
    18721 tgaaaacagc acgttaaatg caacgctatt gcgtgcggaa aattttttac tttgcacgat
    18781 taataatcag tagctgaaag cagtcagcca gtgataaagt agagccaccg cattcatgct
    18841 cctcgtggtt atgtcctgac tagtctttac actctttaca ggaaccattg tcgtacatga
    18901 tggcccaacc aattgagtgt accgctgtcg acatggaatc ttctgcgata caacaattcg
    18961 tatctacaga aggtaactat gtttccacaa tgcaaatttt cccgcgagtt tctacatcct
    19021 cgctactggc tcacatggtt tgggcttggt gtactctggc tttgggtaca gcttccttat
    19081 cctgttctct gctttctcgg cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt
    19141 cgtgaatcta tcgcccgtaa aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa
    19201 cgcgagaaga tgattgccga aaactttcgt tcactcggca tggcgctggt agaaaccggc
    19261 atggcatggt tctggcccga cagtcgcgta cgtaaatggt ttgatgttga agggttggat
    19321 aaccttaaac gcgcacaaat gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg
    19381 tcgctggaac tgggcggccg cgtgatggga ctgtgccaac caatgatggc tacctatcgt
    19441 ccacataata atcagctgat ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa
    19501 gcgatgatcg gcagaaataa tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg
    19561 gtatggtttg ctcccgatca ggattatggt cgtaaaggca gctccttcgc gccgttcttt
    19621 gcggtggaaa atgtcgccac aaccaatggc acctatgttc tctcccgtct ctctggcgca
    19681 gccatgttga ccgtaacgat ggtaagaaaa gcggattaca gcggatatcg tttgttcatc
    19741 accccagaga tggaaggcta cccgacagat gaaaatcaag ccgctgctta tatgaacaag
    19801 attatcgaga aagagatcat gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt
    19861 aaaacgcgcc cggtgggaga atcgtcgttg tacatttaac aaactgctct tactctttcc
    19921 ctcctcaaat cggccataat aacctcatga tttttatagc ttttttctta agttttttcc
    19981 ttcgctgaat atctaattat taccgtcaga aattaaactg tcgccgatat gagacacttg
    20041 taagttacta aaagtaaata agaaaatgcc tcttgtctcg tttcagtttt aggcgtacat
    20101 tagcgccgtc tcgagcagtt tagctcagaa ttctggggtg atgcccacgg taaaatacag
    20161 ggcttttaag gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc
    20221 aggcgtaaca gtaattacgc ggagagatgt aaagtgaaat atttctttat gggcatttct
    20281 tttatggtca tcgtttgggc cggtactttt gccctgatga tctaaaagca aactgtgcaa
    20341 tgaaaaacag gagccatcag gctcctgttt gcatttctga cgacggtgtt tattccgcgt
    20401 tttcgtgaat atgtttgctg ctgacgggta gtaaaccgtc ggcacggaac atcgctttaa
    20461 tccctctgat ggcctgacga atacggtcgt ggttttcaat cagtgcaaag cgaacatgcg
    20521 tatcaccata gtcgccaaag ccaatccccg gcgagacaca aaccttcgct tcgttaagga
    20581 gcttcttggc aaattccagc gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga
    20641 catacatcga agccttcggc atttcgacca tccagcccgc ttcatgcagc cctttaacca
    20701 atacatcacg acggcgtttg tactgttcgg caatgtcgcg cacgcactgt tgatcgccct
    20761 cgagtgccgc aatcgccgcc acctgcaacg gcgtaaaagt gccgtaatcg tgatagcttt
    20821 tgattcgcgc cagggcactg accagcgttt tgttgccgac cataaagccg atacgccagc
    20881 ccgccatgtt gtagcttttc gacagcgtaa agaactcgac cgccacatcg cgtgcacccg
    20941 gcacctgcat aattgacggc gctttccagc catcgtagac gatatcggca taggccaggt
    21001 catggaccac cagcacatcg tagcatttcg ccagcgccac aaccttttcg aagaactcca
    21061 gttccacgca ttgcgcggtt gggttcgacg ggaagccgag gatcatcatc ttcggtttcg
    21121 gataactttc acgaatggcg cgctccagtt cgttgaagaa atcgacacct tccaccagcg
    21181 gcactgagcg tacctgcgcc ccggcaatca ccgcgccata aatatggatc gggtaacttg
    21241 gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc
    21301 cctctttcga accaatagtg acgatggctt ctgattccgg gtcgatttca acgtcgtagc
    21361 gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt
    21421 aaccatgcgt gtccgggcgc tgggccacag tacataattt ttcgacgata tgcggcggag
    21481 tcgcaccgtc cgggttaccc atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag
    21541 ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa
    21601 agcgacgttc agggcgagtg tcagccataa tttcctcaga ttaacgttag cgcccggacc
    21661 gtccgagcga cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct
    21721 gtccagcgtt ttagaacatt tttatcaatg gatttaaatg aggaactcca cttcaaaaac
    21781 aggaaatttt tctacgctaa caagaaaatc accaccaatt ttattaacat tataatttca
    21841 ataaatttac cagccagaag tgcattatca agagatacat tgctaataca ccatttatct
    21901 tttaatcccc ttttaaattt atggtttttc ctcaaaaacg gcctgctggt catcagcctt
    21961 caggttacct atcatagagg tttaatcctt attcagagtc acccgtgcac gaaatattca
    22021 acatgctgct ggcggtcttc gatcgggcag cgttaatgct tatctgcctg ttctttctca
    22081 tccgtatccg cctgtttcgc gaactgttgc acaagtcggc gcactcccca aaagaattgc
    22141 tcgccgttac cgccattttc tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg
    22201 tagaaggctc gctggtgaac gtacgtatta tcgcggtgat gtccggcggg attctgtttg
    22261 gcccgtgggt aggcatcatt accggcgtga ttgcgggtat tcaccggtat ttaattgata
    22321 tcggcggcgt gacggcgatc ccctgcttta tcaccagcat tctggcgggt tgtatatcag
    22381 gctggataaa cctgaaaatc cccaaagcac agcgctggcg ctggatatcg ccaacaaaac
    22441 gctaccgctg tttcgccatg tcaatagcga gtcattacgc aaggtctgcg aaattatccg
    22501 cgatgacatt cacgccgatg cggtggcgat tactaatacc gatcatgtgc tggcctatgt
    22561 tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca
    22621 ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga
    22681 gattcattcc atgctggtga tcccattgtg ggagaaaggg gtcgtgaccg gaacgctgaa
    22741 aatttactac tgccacgcgc atcagatcac ctcgtcatta caggaaatgg cggtcggtct
    22801 gtcgcaaatc atctccacgc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc
    22861 aaataaggca gagattcgcg ccctgcaaag caaaattaat ccccattttc tgtttaacgc
    22921 tctgaacgct atttcatcgt caatccgtct gaatccggat accgctcgcc agttgatctt
    22981 taatctgtcg cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat
    23041 caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggcga
    23101 caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct
    23161 gatccagccg ttggtggaga acgccattgt ccacggtatt cagccttgca aaggtaaagg
    23221 cgttgtcacc atcagcgttg cagagtgcgg aaatcgggta cgcattgcgg tgcgagatac
    23281 cgggcacggc atcgttccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa
    23341 taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca
    23401 tatccgccgc ctggagccgg ggacggaaat tgcgttttac attcctaacc aacgcacccc
    23461 agtcgcctca caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt
    23521 gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag
    23581 atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac
    23641 cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg
    23701 caaaacatca gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa
    23761 catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag
    23821 tcacgtatta ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc
    23881 agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag
    23941 cgaataatcg tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc
    24001 tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc
    24061 aaactgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa
    24121 atacgcgaaa tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat
    24181 tttgaagtgc cggtcagccg cagcaaagtg aaagaatttc gccagttaat gcatctttaa
    24241 tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg
    24301 ttcagcggcc tagtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct
    24361 gcctcgttac cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc
    24421 ggcgaatacg gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg
    24481 tttattccgg cacgcgcggc taatttaccg atcaacaaca aaccggtgat gctgttaagc
    24541 ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg
    24601 ttgcatcctg ctcgccagac ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg
    24661 cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg
    24721 gtgttaagtt tgctgcatct ttgccgcaac gtggtgaata tgccgccggg taattcacaa
    24781 ccgcgcggcg attttctcta tcacagcatt tgtaactggg tttaggataa ttatgcccag
    24841 ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa
    24901 ctgtttgctc agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg
    24961 aaggcgagga tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc
    25021 ggttttccgg atagcgacta tttttgtcgc gttttccggc gtcagtttag tctgacgccg
    25081 ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg ttaattctgt ttccagcagc
    25141 gtcaggatac tttgtgcatc ttgcgcggca aacaacgact ggcggaagtt tttattcacc
    25201 agtttacgcg ccagctgcga gaagactttc acatgattca tcccttcgtt agcacccagc
    25261 gtcagcatga tcaccagttc gacttcgccc atttctgact gccagtcaac cggtttcgcc
    25321 agccgggcaa tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgct
    25381 acgccaaaac caacgccggt ggtaacaatc tcttcccgct gccagacatc ttcttccagc
    25441 tcaaacggat gttcagtgcg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc
    25501 tgctctttat tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga
    25561 acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat
    25621 tcacatgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt
    25681 ttcaccgccg gaatacgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt
    25741 agcggcagat aacggctttc accgcccagt tcaccgcaaa tgcctaccca tttgccccgc
    25801 tgatgcgcag tggtaactat ttgctgcaac atgcgcagga acgatggcgt aatcgggtta
    25861 tatagcggcg atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg
    25921 ttggagccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtagcacacc
    25981 gacggcactt ccaccatgat cccaagcgta atcgtctctg catgacgcag gccatcgcgc
    26041 ttaagctcaa cgatcgcttt ttggatctcg cctttcaccc ataagatctg atcgaggctg
    26101 tgaaccatcg ggatcatcaa ctgggcgttg ccgaaactgg cggcgcgcaa aatggcccgc
    26161 agttgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg
    26221 aacgggttct cttcctgggg aatattcaga taaggaatgc ttttatcgcc gccgatgtcc
    26281 atcgtgcgga agataatcgg cttgtcgccc gccgccagta gcacctgctg gtaggcttca
    26341 aattgctcct gctcgtccgg cgcgctgtcg cgatccatat agagcatttc ggtacggaac
    26401 agcccgacac cttccgcgcc gttggcaaac gcgcctggcg cttccagagc ggtgccgata
    26461 ttcgccgcaa tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcagcctgc
    26521 gcctgttgtt tttggcgttt atccgccagc gtctgcgcga cctgataata accgctcacc
    26581 gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcactgc aggttgcccg
    26641 gcataacggg caatcgcatc cagcggcaag ccactcagta ccgggatcgc cgaggcacgg
    26701 gccagaatca gtgtatgcga ggtgcgcccg gttttctcca gaatcatacc cgcaagattt
    26761 ttcaaatcaa ggctcaaaaa ctggcttggg gttaaatctt cagccaccag aatggtcggt
    26821 ttttccagca ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt
    26881 tcgctgatat cgcgaatgtc gctgacccgc tcgcgcagat aatcgctggc agaagcagat
    26941 agtttggcgc aaacctgctc catattgctg atgatcgccg cccccagtcc ctgatgctgt
    27001 tctgtcatca ggcgacggat attgcctgca aattcatcat cctgaatcag cgacaaatga
    27061 gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctgatt cagttgctcg
    27121 gcaagcgttg ccaggctgtg ctccagccgg gtggaatctt gcgcactggc ggggattgcc
    27181 cgataactgt cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt
    27241 gccagcacat tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga
    27301 tgcgccgtca gttctgcctg cgtcggctga acgctgtcgc tgtcgataaa gcgcaccagg
    27361 atgtactctt ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg
    27421 caactgtcat taaataaggt gccagttcca atcaacgcca gcgagctttt agcatcagcc
    27481 tttgcgttct ggcgatggtt gataaaggtg atttcgcttt gccactggct gcactgttct
    27541 ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc
    27601 gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc
    27661 gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg caaaatgtcg
    27721 cggcaatcgg caatcgatgc cgcgcaatgt ccgtggcggg tggcaggccc cattaccaat
    27781 gtgggtacgc cagtaccggt taagtgcacc gctccaccgt ccgtgccgcc gttactgaac
    27841 atatccgcct gcaacggaac gccaatttct gccgccacgg tttcaatcca cgcggtaagt
    27901 tttggcggcg caatcagcga tttatcgctt aacaccagca tcggcccgtt accaatctga
    27961 cgatggttag ccgcgccata atcaaagttt ttcgcccagc aggcggtatc aagcacaatg
    28021 gcgacgtccg gcgacaccgc gcgggtagca gtttgcccgc cgcgtaatcc cacctcttcg
    28081 ctggaacttg ccaccagcca cacttccgca ggcagttcgg cgtcgtgcag ctcacgtagt
    28141 aacgtcacca gcagatagca actgaggcga tcatcaaagg ctttgcccat cacccgctgg
    28201 tgagggagga cctgaaaagt ggtatcgaag gtgacgcgat cgcctggacg aattcccgcc
    28261 tgcatcactt cgtcataggt gcgcgcgcca atgtccacgc gcatggcgct aacgtcattc
    28321 ccctgccggt cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc
    28381 accggctgca gctggcgggc agccatgcgc acgttgccaa ccggcagcac gtcaatagcg
    28441 ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgggc gcagatcatc
    28501 accttcggac ctgtcgattc attgaggcgg atcagcaccg atcccagacc atcaaatcgc
    28561 acttcttttt gcaggcgagc cgcttcttcc agcaggatct gccgcacttc ctgttccgag
    28621 gaggcgatcg catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc
    28681 ccgtgagcaa cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca
    28741 gcacaacatc ttcgatgcgc acgccccctt gccctagcaa ataaatcccc ggctccacgg
    28801 tcagcaacat gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat
    28861 ggacttcaat gccgatagcg tgaccagtgt tatgaccgaa atagtcgcca aatccagcct
    28921 cggtaatgac ccggcgcgcg gcgtcgtcaa tctgctggca gcgcacgccg gggcgaattg
    28981 cagagattgc cgcgagctgt gcctgcagga caatctgata gacgtcaaac agcgggtgag
    29041 attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca gcagtagccc
    29101 tgatacagcg caccaaaatc gagagtgaca aactcgcccg ctgcaacaat cttgtcgctg
    29161 gctttgccgt gcggcagcgc cccgcgccag ccgctggcga caatggtgtc aaaagaggct
    29221 ttttctgcgc cctgctggcg cataaaccac tccagttcag cggctatctc gcgctcgctc
    29281 atccccgcct gaataaagcg gcgaatatgc tccgctccgc gatcggcaat cccacaggcg
    29341 aggcggatta tctccacctc ctctggcgtt ttgatttgcc gcagcacatc cggcgtggcg
    29401 ctaaccagtt tcgcattgag ttcagacttc cagcggtgcg cggtttccca actcacctgc
    29461 tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt gacgatagtg
    29521 gtaagcgtgt tcgttgcgtc aagcaaatgc agctggtagc cttgcgcacg ggcttccacc
    29581 tcaacgaaat agcgcgaatc taccagaatg tgcgcacttt cacggctaat aaccacatag
    29641 cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga gagaagcacc
    29701 gcatccagtt gttgcacctt aagccagttg cgcagcgaag cgagtaatgt catctggatt
    29761 ttccttctta caggctatcg attaacaatt tgcctttgcg gaacatcatc agacgcagga
    29821 acaccaccat cagcgcagta atgattgccc ccagcgcgat ccccgccata taaacgccca
    29881 agttagtgac cagcggccat gcccagatag cggattccgg gaaccattgc actgcgccca
    29941 gccagacagc tgcggttgag ccgacaatcg cgccgaccat atatgacgga atagcggtga
    30001 tggggctttc cagcgcaaac ggaatcgcac cctcactaat ccccataaag gccaggaaca
    30061 tcgcggtctt accctgcgga taaagttgag cgttgaacag gcgtttgccg gttaaacgac
    30121 ggtcaataat ggtcgccaga cccagaccaa tcggcggaat aacgatagcg atagaacgag
    30181 cggtgaccgg taatacgtgg tcagtggtaa agctgaaggc aacgaaacct gcggctttgt
    30241 tgatcgggcc accaaggtcg attgctgtcg ccgcggcgat ccccattgcg tacatcagcg
    30301 cgcccttctc acctgcggca gtcagcacag tacggatacc gccgttgatc cagccaccaa
    30361 agggagtgat gacgtagtac atcgccagca tgacaaaaat ggcggaaaga atcggtagca
    30421 aaaatgtggt tttgaacgct aacaggaaat ccggtaactg gattttctgg ttcatccatt
    30481 tcacaaggta accggcaacg atagaaataa tcagcgcacc aatgaaggtg gacggcaccg
    30541 gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt cagcagttgc gtcggctggg
    30601 tagacatcaa cccgccgata aaaccagccg ggaacgccag tttgccaccg atagagttcg
    30661 ccacaaatgc agcaaacatc ggaatggcaa aaccaaacaa cacgccgccg aaggactgcg
    30721 acagccaggc aaatttaagt aaggagaggt cgaaaccgga gaatttcccg ctattaagag
    30781 catccatgat gccgatctca gcaggaattt taagccagct ataagcaatc agctggctga
    30841 aagcgaggat cacaccgccc ataattaagg tcggcaccat gcgtgaaatc cctgacatca
    30901 catgctgcgg gagttcaccc cagaagctgc ttttagaggc ctgcggattc ttaactgccg
    30961 ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg gccataacaa
    31021 ttatccctgt cgaatattac tgttgttcag aggcaatcat ctcttcgatt tctttgatga
    31081 tgcccgcagc gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg cgtgattcga
    31141 aacgttcgtt atcttccggg gtaactgcca cggaatggat gatgatggtc gcttcggcga
    31201 tatcctgcgc cgtcaggcga ttctggatgc cgtccgcgcc ctgggtttca attttcactt
    31261 cataaccggc ttctaccgcc gcttcttcca gcacctgagc ggccataaaa gtgtgagcca
    31321 ggcccattgg gcaggcacat aaggcaatca gtttcttact catcgttaat tcctcgttca
    31381 gtgatagttg actagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac cagacaaata
    31441 atgcatttac tggagaatta atcccgctgt acggaagtgt gacgcaggtc ggccttgtga
    31501 ggggacacaa agaaaaattg aattaactgt atgatttaaa agattatcgg gagagttacc
    31561 tcccgatata acaggaagga tttacagaat gtgacctaag gtctggcgta aatgtgcacc
    31621 ggaaccgaga agacccggat agtcatggac gatgagatac accggaatat catggacata
    31681 ttctttaaag cgccctttat cttcaaatgc ggcacggaaa ccggaggctt tgaagaactc
    31741 aaggaagcgc ggcacgatac cgcccgcaat aaacacgccg ccaaatgtcc cgagattgag
    31801 cgccagattg ccgccaaaac ggcccataat gacgcaaaac agcgacaatg cgcggcggca
    31861 atcggtgcag ctgtcagcca gcgcgcgttc ggtaatatct tttggcttga gattttctgg
    31921 caggcggttg tcagctttca caattgcgcg atacaaattc accagcccag ggccagaaag
    31981 cacgcgctcc gccgaaacat gaccaatttc cgcacgcaat atttcgagga taatggcctc
    32041 ttcttcacta ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc ttacccaacg
    32101 cttatcgaca tggaccagat gcgcaacccc aagccccgtt ccggcaccgt aaaccgcaat
    32161 aggcttacct tcgaccggtt ctgcgccacc aaactgaatc agatgctctt ttttcagcat
    32221 cgggatcgcc atcgatacag cggtaaaatc gttaataatt tccagatggc taaaaccgag
    32281 attctttttc atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg ccacccagtc
    32341 accggtaatt gggcaagcga tggcaataca gccgtctttc acctcgacct tatgttcttc
    32401 aagataaacg cgaatgaccg cttcgaggct ggggtaatca agccctgaat aggtcttagc
    32461 ctgcgagatt tcaccactgg caatatcaca cagagcaaga cgtgcgttgg tgccgcccac
    32521 atcaccgact aatgcatact ttgtcattct tcaactgctc cgctaaagtc aaaataattc
    32581 tttctcacac tgtaaatacc tgggggcata acaacaacgc cggaaaggca ggctccctgt
    32641 aaatatcgat ctgggtcaca caattacttt atcgtttcag caccaattgc agcgatgcct
    32701 ttttgcaagc tgggcaaact aagtatctga ccccgcataa ggaatagaac atgctccatc
    32761 cgcgagccag aaccatgttg ttattatcgc tccccgccgt ggcaattggg attgcgtcca
    32821 gtcttattct gattgtggtg atgaaaatcg cctcggtatt acagaatttg ctctggcaac
    32881 gactgccggg aactctgggg atagcccagg attcacccct ctggatcatc ggtgtattaa
    32941 cgctaacggg tattgcggtg gggttggtta tccgtttcag ccagggtcat gccggaccag
    33001 accccgcctg tgaaccgctg atcggcgcac cggttccgcc ctctgcgcta cctagactta
    33061 tcgtagcatt aattctcggt cttgctggcg gcgtcagcct ggggccggaa catccgatca
    33121 tgaccgtcaa tatcgccctt gccgtcgcca ttggcgctcg tctgttaccg cgcgtcaacc
    33181 gaatggagtg gactatttta gcctctgccg gaaccatcgg cgcgctgttt ggtacacctg
    33241 ttgcggcggc gttgatattt tcgcaaacct taaatggcag tagtgaagtt ccgctatggg
    33301 atcgtctttt tgcgccgtta atggcggcag cagctggtgc acttactacc ggattatttt
    33361 tccatcccca tttttcactg cccattgcgc attacggaca gatggaaatg accgatattc
    33421 tcagcggtgc aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc gtatggtgct
    33481 taccacggtt gcacgcgatg atgaatcaaa tgaaaaatcc ggtgctcgtg ctgggtattg
    33541 gcggatttat tctcggaatt ctgggggtta ttggtggacc agtttcgctg tttaaagggc
    33601 tggatgagat gcagcagatg gtggcaaatc aggctttcag caccagcgat tactttttgc
    33661 tggcggtaat taaacttgcc gccctggtcg ttgctgccgc cagtggcttt cgcggtgggc
    33721 gaatcttccc ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat gagcacgttc
    33781 ccgccgttcc agcggcaata accgtttcct gcgctattct cggcatcgtg ctggtggtaa
    33841 cacgcgatgg ctggttaagt ctttttatgg cggcagtcgt tgtacccaat accacattgc
    33901 taccgctgct ctgtatcgtc atgcttccgg catggctgtt attagcaggt aagccgatga
    33961 tgatggtcaa tcgtccgaag caacagccac cccacgataa tgtttagcaa aaaatgctct
    34021 cctttatgat ttaagagtta tggcgatgat acggggcttt acctccccgt aatattgcgt
    34081 taacaggccg ctgacggctt cgatcgtgaa ggagaataac aatgttcagg tcactgtttc
    34141 tggcggccgc cctgatggca tttaccccgc ttgcagcaaa tgcaggtgaa atcaccctac
    34201 tgccatcgat caaattacaa attggcgatc gcgatcatta cggtaattac tgggacggtg
    34261 gtcactggcg agatcgtgat tactggcatc gcaattatga atggcgcaaa aaccgctggt
    34321 ggcgtcatga taatggctat catcgtggct gggataagcg taaagcatat gagcgtggct
    34381 atcgtgaagg ctggcgcgat cgtgacgatc atcgcggaaa aggccgcgga catgggcacc
    34441 gccattaaac gcgtcctcca atggagcaca atgcctgatg cgacgctagc gcgtcttatc
    34501 aggcctacaa accggctctt taacgcgtag ggcggataag gcgtttacgc cgcatccgcc
    34561 aatagtgcca gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc
    34621 agatattcag cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc
    34681 tgttcaccag atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg
    34741 ccagggcgat accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta
    34801 atcccatcag aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg
    34861 ggatatggaa gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg
    34921 ttgaggacag tccggcagca accagactta acccaaagac cgttgccgca gcatggctta
    34981 acagcggttg cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa
    35041 agtggaacgc cgccgcagct gtagccatca tcaccagatt gacaaaaccg gcaatcgtca
    35101 tggcgatagc cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta
    35161 aatgctgagt gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca
    35221 cgcctgctgc caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac
    35281 ccagctgcgc caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa
    35341 acaacagtaa cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta
    35401 aaatcaggaa agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa
    35461 tgagtttaaa accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa
    35521 tttctgcctg aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct
    35581 ccgccagatt tttaccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg
    35641 ccatcaggtt ggcccaaacg acaacccaca gtagctgata gccgaagcta gcacccgcct
    35701 gaatattggt cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc
    35761 ccattaatgc gagcctcatc ttacgcgccg cccgtccgct gctactctca acgcgatagt
    35821 tcgtcatctt gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ctaaacgaga
    35881 atgattatca aattcattta aatggattgt ggtgatttct ctgatagacc aagattatga
    35941 ttacgaaaag attgatgact tgaatgttat ggttatgttt aatgttagca catttacata
    36001 actttcagct tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc
    36061 tgtcacagga cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag
    36121 aatgtgcacg gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat
    36181 tttgtactgg cacttgccgt tgttgtgatt ctcgcactgc tggtaagcag cgaccgcaaa
    36241 aaaattcgta tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc
    36301 ttcctgaact ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa
    36361 ctgctcggat ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc
    36421 ctggcattct tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt
    36481 attctccagc acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc
    36541 aaagtcaacg gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt
    36601 cagtcagaaa actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg
    36661 tacaccatgg ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg
    36721 accatgctgg aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt
    36781 atcgtgctgt cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg
    36841 tccaacctgc acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt
    36901 ttcaaagttg ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg
    36961 ctgaacgcac tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc
    37021 atcctgggct acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca
    37081 ctgcaagtgg gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg
    37141 gatctgcaga aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc
    37201 ctagtttcct tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg
    37261 aatgaagagc aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc
    37321 ctggtgagtg tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa
    37381 agccggggat cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac
    37441 caatcaaata cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt
    37501 gcgtctcgac aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg
    37561 atctcacaat catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa
    37621 ttttgatgat atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa
    37681 agtcatcaat cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc
    37741 tttctgcgtt agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga
    37801 tgtgataacg cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga
    37861 gcggcattaa attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc
    37921 acttcaacaa ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa
    37981 taaggggcag aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga
    38041 tctcatcata accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac
    38101 cctgatctaa cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg
    38161 aaaccatctc ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg
    38221 ccagccagct taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc
    38281 cccaggcagc accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat
    38341 tcaggatgtt aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca
    38401 gcagttcact acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg
    38461 tacggcaaat tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa
    38521 actcaagatt atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt
    38581 gttccaacgc acgaaagttg ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc
    38641 agcggcgatt aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc
    38701 taaaggaaat cagcaccgcg agaataaatg ccagcgaata ttcggtttcc accccttgca
    38761 aaaagttctg attgtaattc agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa
    38821 acggatagcg aagattaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt
    38881 aaccggcaat aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg
    38941 ctgccagcca gcttaaggta aatgcgagtg agatagtaga aaagcatgtt gtaaatcagc
    39001 actgcggtga aaaggcttag taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa
    39061 aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat gctgcatttg
    39121 atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata tcggctggtc
    39181 aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacg
    39241 acaaacgtct ggctgccgag catgaccgca gtctgtaaaa aagagagatg aaagttccac
    39301 agattggtgc aaaacatccc gcataaaacg ggaacgacac cgcgccagcc aaagataaac
    39361 aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact ggaatggata
    39421 atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct
    39481 agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc
    39541 tcactgatcg gggttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca
    39601 ggggccagcc tgttgccgat ttttcatcca atgatgcttt caccaggcgg cgctaacccg
    39661 gacgtagact gtattacaaa agcggcaaaa agcagagaca aaaaaccccc gctttgcagc
    39721 gagggttg
//
LOCUS       DAJTYL010000024        39350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYL010000024 DAJTYL010000000
VERSION     DAJTYL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.024"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..586)
                     /locus_tag="OO572_001231"
     CDS             complement(<1..586)
                     /locus_tag="OO572_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6660301.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALI"
     gene            832..1458
                     /gene="gstB"
                     /locus_tag="OO572_001232"
     CDS             832..1458
                     /gene="gstB"
                     /locus_tag="OO572_001232"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6660302.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1455..2569)
                     /gene="yliI"
                     /locus_tag="OO572_001233"
                     /pseudo
     CDS             complement(1455..2569)
                     /gene="yliI"
                     /locus_tag="OO572_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2680..3063)
                     /gene="bssR"
                     /locus_tag="OO572_001234"
     CDS             complement(2680..3063)
                     /gene="bssR"
                     /locus_tag="OO572_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6660303.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3276..4601
                     /gene="rimO"
                     /locus_tag="OO572_001235"
     CDS             3276..4601
                     /gene="rimO"
                     /locus_tag="OO572_001235"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6660304.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4648..5976)
                     /locus_tag="OO572_001236"
     CDS             complement(4648..5976)
                     /locus_tag="OO572_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6660305.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(5984..8326)
                     /locus_tag="OO572_001237"
                     /pseudo
     CDS             complement(5984..8326)
                     /locus_tag="OO572_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8504..9415)
                     /gene="gsiD"
                     /locus_tag="OO572_001238"
     CDS             complement(8504..9415)
                     /gene="gsiD"
                     /locus_tag="OO572_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6660306.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9418..10338)
                     /gene="gsiC"
                     /locus_tag="OO572_001239"
     CDS             complement(9418..10338)
                     /gene="gsiC"
                     /locus_tag="OO572_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6660307.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10356..11894)
                     /gene="gsiB"
                     /locus_tag="OO572_001240"
     CDS             complement(10356..11894)
                     /gene="gsiB"
                     /locus_tag="OO572_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6660308.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(11914..13785)
                     /gene="gsiA"
                     /locus_tag="OO572_001241"
     CDS             complement(11914..13785)
                     /gene="gsiA"
                     /locus_tag="OO572_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6660309.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13772..14737)
                     /gene="iaaA"
                     /locus_tag="OO572_001242"
     CDS             complement(13772..14737)
                     /gene="iaaA"
                     /locus_tag="OO572_001242"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6660310.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14941..16176
                     /gene="moeA"
                     /locus_tag="OO572_001243"
     CDS             14941..16176
                     /gene="moeA"
                     /locus_tag="OO572_001243"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6660311.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16176..16925
                     /gene="moeB"
                     /locus_tag="OO572_001244"
     CDS             16176..16925
                     /gene="moeB"
                     /locus_tag="OO572_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6660312.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17104..17766)
                     /gene="fsa"
                     /locus_tag="OO572_001245"
     CDS             complement(17104..17766)
                     /gene="fsa"
                     /locus_tag="OO572_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6660313.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17897..18796
                     /locus_tag="OO572_001246"
     CDS             17897..18796
                     /locus_tag="OO572_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6660314.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18802..21234
                     /locus_tag="OO572_001247"
     CDS             18802..21234
                     /locus_tag="OO572_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6660315.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21380..22195
                     /gene="ybiV"
                     /locus_tag="OO572_001248"
     CDS             21380..22195
                     /gene="ybiV"
                     /locus_tag="OO572_001248"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6660316.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22347..23612
                     /locus_tag="OO572_001249"
     CDS             22347..23612
                     /locus_tag="OO572_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6660317.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23853..25445)
                     /locus_tag="OO572_001250"
     CDS             complement(23853..25445)
                     /locus_tag="OO572_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6660318.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25664..26584
                     /gene="ldtB"
                     /locus_tag="OO572_001251"
     CDS             25664..26584
                     /gene="ldtB"
                     /locus_tag="OO572_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6660319.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26642..27760)
                     /locus_tag="OO572_001252"
     CDS             complement(26642..27760)
                     /locus_tag="OO572_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6660320.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27757..28224)
                     /gene="mntR"
                     /locus_tag="OO572_001253"
     CDS             complement(27757..28224)
                     /gene="mntR"
                     /locus_tag="OO572_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6660321.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28410..28538
                     /gene="mntS"
                     /locus_tag="OO572_001254"
     CDS             28410..28538
                     /gene="mntS"
                     /locus_tag="OO572_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6660322.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28810..30393
                     /gene="opgE"
                     /locus_tag="OO572_001255"
     CDS             28810..30393
                     /gene="opgE"
                     /locus_tag="OO572_001255"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6660323.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30441..30956)
                     /gene="ompX"
                     /locus_tag="OO572_001256"
     CDS             complement(30441..30956)
                     /gene="ompX"
                     /locus_tag="OO572_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6660324.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31009..31074)
                     /locus_tag="OO572_001257"
     CDS             complement(31009..31074)
                     /locus_tag="OO572_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660325.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31309..32196
                     /gene="rhtA"
                     /locus_tag="OO572_001258"
     CDS             31309..32196
                     /gene="rhtA"
                     /locus_tag="OO572_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6660326.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32495..32998
                     /gene="dps"
                     /locus_tag="OO572_001259"
                     /gene_synonym="pexB"
     CDS             32495..32998
                     /gene="dps"
                     /locus_tag="OO572_001259"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6660327.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33402..34148
                     /gene="glnH"
                     /locus_tag="OO572_001260"
     CDS             33402..34148
                     /gene="glnH"
                     /locus_tag="OO572_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6660328.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34287..34946
                     /gene="glnP"
                     /locus_tag="OO572_001261"
     CDS             34287..34946
                     /gene="glnP"
                     /locus_tag="OO572_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6660329.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            34943..35665
                     /gene="glnQ"
                     /locus_tag="OO572_001262"
     CDS             34943..35665
                     /gene="glnQ"
                     /locus_tag="OO572_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6660330.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            35926..38150
                     /gene="ybiO"
                     /locus_tag="OO572_001263"
                     /pseudo
     CDS             35926..38150
                     /gene="ybiO"
                     /locus_tag="OO572_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38147..39073)
                     /gene="rlmF"
                     /locus_tag="OO572_001264"
     CDS             complement(38147..39073)
                     /gene="rlmF"
                     /locus_tag="OO572_001264"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6660331.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
BASE COUNT         9765 a        10400 c         9603 g         9582 t
ORIGIN      
        1 ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat
       61 ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg gcataaccat
      121 tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc gcaatacagg
      181 cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc
      241 ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat
      301 ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa
      361 ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca tccgcattgc
      421 cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc
      481 tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg
      541 cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag
      601 tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt aataacgcac
      661 acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca atatcctgcc
      721 gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta actttacttc
      781 accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag tatgattacg
      841 ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct cgaagaactg
      901 gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca cgatgctgat
      961 tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga aagtgatctc
     1021 attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc
     1081 ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac
     1141 cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac accaccggaa
     1201 gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct gtttgccctg
     1261 ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat
     1321 atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg gacaccgcgt
     1381 ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg
     1441 atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact ggactcgtcg
     1501 gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg
     1561 tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca
     1621 tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat ttatcgctgt
     1681 tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg
     1741 tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc
     1801 aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac
     1861 cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc gccattcctt
     1921 gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc tttataaaag
     1981 gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat
     2041 ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt
     2101 taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc
     2161 ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg ccataaccca
     2221 cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag atgcggcgag
     2281 actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg tgcgcccaaa
     2341 cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag tgacgcaact
     2401 cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc agtgcccagg
     2461 gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc gcccagagag
     2521 cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata acgcctccgg
     2581 attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt ttacaaaaac
     2641 ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct caagcatggc
     2701 gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt caagcttatc
     2761 ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac atactgcggc
     2821 tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat ccagatgaag
     2881 attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt tatctcgtag
     2941 atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca cgtatgcagc
     3001 gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc tgtcaacgaa
     3061 catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc tattttttga
     3121 gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac atttccttcg
     3181 cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta gaatccctgc
     3241 cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact ccccagccga
     3301 aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag cgtattctca
     3361 ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgct aacatggtga
     3421 tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa gccattggtg
     3481 aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca aaagaagatc
     3541 agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc tatgagcagg
     3601 ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc ctgagcctgg
     3661 tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa atttcggaag
     3721 gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac ctgatgagcc
     3781 gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc gttaaagaga
     3841 ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat cgtactggct
     3901 tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag ttatcgaaac
     3961 tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac gacgtcatcc
     4021 cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag cacgccagcc
     4081 cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg gcgcgcatca
     4141 aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt gtcggcttcc
     4201 ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa gcacgtctgg
     4261 atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat gccctgcctg
     4321 accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag ctgcagcagc
     4381 agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg attatcgacg
     4441 aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa atcgacggcg
     4501 cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt gtgaaagtcg
     4561 agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag gccgggcaaa
     4621 atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc tgccgttttt
     4681 cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg tagtacattc
     4741 atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct ggaaatgaac
     4801 gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa tcaccgcaaa
     4861 ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg atttaaggat
     4921 tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt cgttaatgtt
     4981 tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca gcgcttttaa
     5041 atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga acgccgagtt
     5101 atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc catccagtac
     5161 gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat taatcactct
     5221 gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg caaagaaaat
     5281 aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt acaatccggt
     5341 cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt ttttagcatt
     5401 atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca ggttctcgct
     5461 gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca aaaatatcag
     5521 actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt caaaagagat
     5581 aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg cacaataatt
     5641 tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag gaccgacatc
     5701 gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa ccaacagctg
     5761 ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc gatattcagc
     5821 aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat acacccgttc
     5881 ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa aaatcagcag
     5941 actgactgta agtacgaact tattgattct ggacatacgt aaactactct tttactaatt
     6001 ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac ccctgagcct
     6061 gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg ccttccgcaa
     6121 tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt tttcccgcag
     6181 taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga ttaatttcat
     6241 aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc cgtacccctt
     6301 gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg atcacggttt
     6361 cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg atggtggtaa
     6421 aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata tgcagtaaga
     6481 aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt gccgttttaa
     6541 taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta atgaacttca
     6601 gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc gcgctcagaa
     6661 tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg ccaatttcca
     6721 ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg agtttcgcgt
     6781 catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc gccagtttca
     6841 gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg gcagacttat
     6901 ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc gctattttac
     6961 gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca ttgaatgtca
     7021 cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc ctgacgattt
     7081 tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac agtttgatga
     7141 tccccacagc cagatactga ttattgttgg actcaataat cgccatattt ttaataaaac
     7201 cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca atttcggcat
     7261 ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg ccttcgcgaa
     7321 ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta ttaaccagct
     7381 cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat accaccagca
     7441 acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg ttcaataaat
     7501 taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact gctgatgtct
     7561 ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg ctattataaa
     7621 taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct tgtttcaaaa
     7681 atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac cgccagttta
     7741 aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg atacgtagcg
     7801 taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga ttccagctgt
     7861 tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa tcggcaaaaa
     7921 taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga taaacaacga
     7981 ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt accagtccca
     8041 ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga atctcctctg
     8101 tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca atatctttaa
     8161 agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt tttaccgcgc
     8221 gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac agtgctgcca
     8281 ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta atacatttaa
     8341 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
     8401 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
     8461 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
     8521 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
     8581 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
     8641 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
     8701 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga
     8761 cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
     8821 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa
     8881 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
     8941 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
     9001 gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
     9061 ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
     9121 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
     9181 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
     9241 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
     9301 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     9361 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
     9421 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     9481 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     9541 tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     9601 agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
     9661 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
     9721 acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac
     9781 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
     9841 tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa acatccccag
     9901 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
     9961 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
    10021 catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct catcggcaac
    10081 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
    10141 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
    10201 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatgtggac
    10261 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt
    10321 gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
    10381 ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
    10441 agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc ctgcgccgcc
    10501 ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc caggaagtca
    10561 tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag
    10621 gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag
    10681 aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
    10741 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
    10801 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
    10861 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
    10921 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg agttgcatag
    10981 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
    11041 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
    11101 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
    11161 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
    11221 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
    11281 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
    11341 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt cggtgaaatc
    11401 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
    11461 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
    11521 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
    11581 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt gtaagtaatg
    11641 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
    11701 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcattcgcg
    11761 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
    11821 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
    11881 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
    11941 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
    12001 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
    12061 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
    12121 cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga gatacatcac
    12181 cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa acagatacgc
    12241 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
    12301 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
    12361 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
    12421 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
    12481 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
    12541 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
    12601 tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccctgcga
    12661 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
    12721 cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc
    12781 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
    12841 ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt cctgtttcgc
    12901 tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc
    12961 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
    13021 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
    13081 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
    13141 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
    13201 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
    13261 ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt gtgcctccgg
    13321 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
    13381 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg ggttcagcga
    13441 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc
    13501 agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca gcattttatc
    13561 gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg cagtcactga
    13621 cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca gactaaaaga
    13681 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat taagattttc
    13741 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
    13801 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
    13861 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
    13921 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
    13981 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
    14041 ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
    14101 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
    14161 ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
    14221 catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccagta
    14281 gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc atgccatgag
    14341 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
    14401 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
    14461 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
    14521 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
    14581 ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca acaatggcag
    14641 acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
    14701 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
    14761 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat
    14821 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
    14881 tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
    14941 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
    15001 gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg
    15061 gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc
    15121 tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc
    15181 tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
    15241 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
    15301 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
    15361 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
    15421 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
    15481 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
    15541 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
    15601 atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc
    15661 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
    15721 accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
    15781 ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
    15841 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
    15901 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
    15961 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
    16021 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
    16081 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga atgggtggaa
    16141 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga
    16201 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
    16261 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
    16321 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
    16381 cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat
    16441 ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
    16501 tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta
    16561 cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc
    16621 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
    16681 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
    16741 aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca atggaagcga
    16801 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga
    16861 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
    16921 agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg catttgctcc
    16981 agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca gatttccaag
    17041 cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
    17101 cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgtcacagc
    17161 ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga
    17221 ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag
    17281 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
    17341 gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
    17401 cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg tcggaatccc
    17461 ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa ctttcaccac
    17521 gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt cagcagtggt
    17581 agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag
    17641 cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc
    17701 cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag
    17761 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
    17821 tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg
    17881 aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg
    17941 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
    18001 ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg
    18061 cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat
    18121 tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca
    18181 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga ccgttctgcg
    18241 cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg gtgaaccctt
    18301 catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag gcattcatac
    18361 tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat
    18421 cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    18481 cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat
    18541 cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat
    18601 taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac
    18661 gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    18721 tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag ggttaaccgc
    18781 gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt
    18841 aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac cgagcgcgcg
    18901 cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg
    18961 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc
    19021 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg
    19081 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc
    19141 gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca gacggtacag
    19201 gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt
    19261 aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctgctg
    19321 ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat
    19381 ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga tatcatgctg
    19441 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat ggccgcgacc
    19501 gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg cgatcttatc
    19561 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg
    19621 attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga tcagtatctc
    19681 tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga acacgccatc
    19741 gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctcc
    19801 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cggacaaaat
    19861 ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat cctcgaatcc
    19921 tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt
    19981 aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat gccggcattc
    20041 aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct
    20101 tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg gggctatcgc
    20161 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg
    20221 cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac
    20281 ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta ctacacgcgt
    20341 aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt gcacgatatt
    20401 ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc
    20461 gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg caacagcctg
    20521 gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    20581 gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac
    20641 ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat
    20701 cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg tggtccggtt
    20761 ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt tggcgcgcaa
    20821 actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg
    20881 gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg taaactgcct
    20941 acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag
    21001 aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga agtgcataaa
    21061 ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat
    21121 ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg
    21181 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctctt
    21241 cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt
    21301 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    21361 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg acggtacttt
    21421 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa
    21481 aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    21541 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    21601 acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    21661 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    21721 cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa
    21781 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    21841 ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg gcattatgaa
    21901 acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa
    21961 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    22021 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa
    22081 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    22141 cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca gctgacctct
    22201 ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat
    22261 cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt agcatagttt
    22321 cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac acattgcctg
    22381 ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag cttggcgatg
    22441 tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg
    22501 cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag
    22561 gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag
    22621 gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctgaacc
    22681 gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg
    22741 cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga
    22801 gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg
    22861 gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccttc
    22921 cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct
    22981 ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat ctggcgaaat
    23041 acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca
    23101 ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat atgctgcctg
    23161 gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc
    23221 cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc
    23281 cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg
    23341 aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa
    23401 atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc
    23461 ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc
    23521 cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac ctcaacattc
    23581 acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga
    23641 ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga
    23701 tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata ttacaggtac
    23761 tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc agccgtcgtc
    23821 accccgacag tatcggggtg acggataatc acttactcga tccctttact acgcaggtaa
    23881 tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag aatgcgggtc
    23941 gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt
    24001 tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt cggttcgtcc
    24061 atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg ccctttttcc
    24121 ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag caaacgaccg
    24181 agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact catccattcg
    24241 aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta accaatgcgc
    24301 gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac cagcgttttc
    24361 agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc accgacctcc
    24421 agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt cagaccttcc
    24481 acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa cgggttctga
    24541 cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt
    24601 gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg caactcagca
    24661 atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg ggtcgccgcc
    24721 gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc cagatccgcc
    24781 atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat catggtgctg
    24841 tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag gttgttggtc
    24901 ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag aagcacacgc
    24961 agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac tggaattccc
    25021 acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat
    25081 ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg caaagcatag
    25141 atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat caccgtatcc
    25201 agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat gcgctcgttg
    25261 ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat cttcataaag
    25321 gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg
    25381 acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt actggaaact
    25441 aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat aagtctgaaa
    25501 gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg
    25561 tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca atactctaat
    25621 attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata tgaaattgaa
    25681 aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac
    25741 ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga tcaccattcc
    25801 tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg ggctttccaa
    25861 tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta ctgtactgaa
    25921 cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc
    25981 agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg
    26041 cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg agcataagaa
    26101 agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga
    26161 accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt atgcactcta
    26221 catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg gcctgcgtgt
    26281 aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc
    26341 ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg agccagacgg
    26401 cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt ttgaaggtca
    26461 ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc agccagatgt
    26521 tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg ttcgcctgaa
    26581 ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta tttgccagac
    26641 cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc cacaacagca
    26701 tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc atacgtagcg
    26761 caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt accgcccatg
    26821 ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc acgttactga
    26881 tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa tgactcacgt
    26941 tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg ataaacatcg
    27001 ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc gccagcagca
    27061 caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc agcgccgtca
    27121 gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac tccggtgttt
    27181 gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac aacagcagga
    27241 gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg gcaaacgaaa
    27301 gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc agtagcgaac
    27361 cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc tcacacagtc
    27421 tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg gtcagaaagg
    27481 tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc gtagcaaagc
    27541 ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca cctttggtca
    27601 gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca ggccaggatt
    27661 tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat aactgaaaaa
    27721 aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt
    27781 aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc cttccgcgtc
    27841 gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga ctatctgatg
    27901 tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca cgccacgcca
    27961 ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag ccaccgtcgg
    28021 ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca cttccctgat
    28081 caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg cctctctgac
    28141 ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt tttttgctat
    28201 tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat acatagcgtt
    28261 ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt aacgttttag
    28321 caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat ccctcaagga
    28381 tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt atgcgcgtgt
    28441 ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc gatatggtca
    28501 acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt acgccgcttc
    28561 actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact ttttgtcgga
    28621 tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg gcggctcgct
    28681 ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct ttcccctctt
    28741 cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg tttgttacga
    28801 tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc
    28861 ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt aggttacccc
    28921 ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg gcgaacattg
    28981 cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg ttatttccct
    29041 tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca ctccaccaat
    29101 atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct ggtcggctta
    29161 tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc
    29221 tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt tgttgctccc
    29281 gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta tccggtattt
    29341 cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca agaacggatg
    29401 gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc gaagtatcag
    29461 acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt
    29521 cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc tgactacatt
    29581 gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt agtcgatggc
    29641 aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg
    29701 tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag catcgccaaa
    29761 cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa aaacacgaaa
    29821 gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag
    29881 ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac acaaggcaaa
    29941 tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat gacgcaaacg
    30001 gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag cttctctctg
    30061 gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt gcaatacctt
    30121 gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat ttccagcggc
    30181 gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt aggctttttc
    30241 tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt tatatctgag
    30301 aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga ttacaaccat
    30361 ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc gagaggcgga
    30421 tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca ggtgcctacg
    30481 tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg
    30541 ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc
    30601 gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta gatgcttgcc
    30661 cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag
    30721 tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc
    30781 gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg
    30841 ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc tacggaagta
    30901 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
    30961 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
    31021 tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
    31081 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
    31141 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
    31201 attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc
    31261 cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca
    31321 ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt
    31381 cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc gggtgtcact
    31441 gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg
    31501 cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg
    31561 aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctggag
    31621 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
    31681 gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
    31741 gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc tatttacatt
    31801 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat tggttcgttg
    31861 attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac
    31921 tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct gccttattcg
    31981 ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg
    32041 gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
    32101 cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc
    32161 aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt atggttatgc
    32221 ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata tcgaattatg
    32281 caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt gttcttatca
    32341 atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta ccactattag
    32401 tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta gtctcgttaa
    32461 ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat tagttaaatc
    32521 aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga aaaaagcaac
    32581 agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga ttaccaaaca
    32641 agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc tggatggctt
    32701 ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc agctgggcgg
    32761 tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa gttacccgct
    32821 ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg caatcgtcgc
    32881 taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag atatcctgac
    32941 cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca tcgaataaat
    33001 ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga caggaggccg
    33061 ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct atccccagct
    33121 gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag attgtttttt
    33181 catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg caccagattg
    33241 gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc acgtttttgc
    33301 cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat atgtgaatgt
    33361 cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct gtattaaaag
    33421 tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg gataaaaaat
    33481 tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc gataaatatg
    33541 tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg gattacgaac
    33601 tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac gtcgatctgg
    33661 cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc tctgacggct
    33721 actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg aaaagcgtga
    33781 aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt gattacgcga
    33841 aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac gcctatatgg
    33901 aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt ctgtacttca
    33961 tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa gcgcagcaat
    34021 acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac ggcgcgttga
    34081 aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc agtactgaac
    34141 cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc cctggggaga
    34201 ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga atttgattat
    34261 ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc tggcctgcca
    34321 ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc ggtctggctg
    34381 gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt tggatagcca
    34441 accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc gtccaggtga
    34501 tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac ccatttactg
    34561 cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc acgcgtggtg
    34621 cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt ctttcacgtt
    34681 gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg ccgccgctgg
    34741 gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc ggcgtggcgg
    34801 aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt gagatctgga
    34861 gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt ctgcgtcgtc
    34921 tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc actttggccc
    34981 aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg tggtgattat
    35041 cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac tggaagaaat
    35101 cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag ttgacgagcg
    35161 cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc cgcatctgac
    35221 ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca aagaagaggc
    35281 ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg cacatcacta
    35341 cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg cgctggcggt
    35401 gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg aactgcgcca
    35461 tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg tgatcgtgac
    35521 ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg acaaaggccg
    35581 gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc agcgcttgca
    35641 ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac gcttatcagg
    35701 cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg ataaggcgtt
    35761 cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat atcccttcct
    35821 ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc ccctccccca
    35881 gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg
    35941 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
    36001 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
    36061 aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt aaagagttga
    36121 tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg aagatcgtgc
    36181 cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc agccgccatt
    36241 atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc ggctccccgc
    36301 ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca atgttagcgg
    36361 tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta tatcgcaaaa
    36421 tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag cttccggcga
    36481 tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta tttgtcggcc
    36541 aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa caaagtttgt
    36601 ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg attttttgcc
    36661 caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt tactggggtc
    36721 gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc gtgccgatta
    36781 tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt atgctgtgca
    36841 tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc cagcatatgc
    36901 tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc tttgcgctgg
    36961 tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg ttgttcgatc
    37021 cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg attattggta
    37081 tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc accctctcgc
    37141 cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg ctttcggcgg
    37201 cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg ttgagcgcat
    37261 ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa accgtagata
    37321 tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg gtgctcgcca
    37381 gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc agcgcccgta
    37441 cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt accatcacca
    37501 tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca ggtgccgggg
    37561 cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt atcaccgggg
    37621 tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc gggccgttga
    37681 ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat accggggcgt
    37741 atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc ggtattggtt
    37801 cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc aatcaggcac
    37861 tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg attattggtg
    37921 aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg cgtgtttcgt
    37981 tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc caggtgaaaa
    38041 aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg ctgcctgctc
    38101 cgggcgcgac cccggctgag ccgctgccgc cgggggagcc aacgctttaa cgttggcgat
    38161 taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag cgactctgct
    38221 tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg gtcagagcac
    38281 gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac atcacctgct
    38341 tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca ccgccttcac
    38401 accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc agccccaggt
    38461 tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg aatggcgggt
    38521 tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa atcgccccac
    38581 tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca ctgataatcg
    38641 cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc cagccatatt
    38701 catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc agaatgctgg
    38761 cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga tgaatataat
    38821 ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag ttcgctacgg
    38881 cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga ttggcaaagt
    38941 ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca ggattgacct
    39001 gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc aaccccggtt
    39061 tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca taacgcgaca
    39121 gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta tggtttatgg
    39181 caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt tgtaaaaata
    39241 tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca tcgcgcaggc
    39301 gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
//
LOCUS       DAJTYL010000025        38849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYL010000025 DAJTYL010000000
VERSION     DAJTYL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.025"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..474
                     /gene="uidR"
                     /locus_tag="OO572_001265"
     CDS             <1..474
                     /gene="uidR"
                     /locus_tag="OO572_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6660332.1"
                     /translation="SCAISPGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHF
                     VDYMVESIVSLTHEAFGQRALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQ
                     QKGEISPDINTAMTSRLLLDLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            862..2565
                     /gene="uidA"
                     /locus_tag="OO572_001266"
     CDS             862..2565
                     /gene="uidA"
                     /locus_tag="OO572_001266"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6660333.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            2571..3596
                     /gene="uidB"
                     /locus_tag="OO572_001267"
                     /pseudo
     CDS             2571..3596
                     /gene="uidB"
                     /locus_tag="OO572_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            3635..4900
                     /gene="uidC"
                     /locus_tag="OO572_001268"
     CDS             3635..4900
                     /gene="uidC"
                     /locus_tag="OO572_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6660334.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5126..6634)
                     /locus_tag="OO572_001269"
     CDS             complement(5126..6634)
                     /locus_tag="OO572_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6660335.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(6735..7910)
                     /gene="manA"
                     /locus_tag="OO572_001270"
     CDS             complement(6735..7910)
                     /gene="manA"
                     /locus_tag="OO572_001270"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6660336.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8109..9755
                     /gene="fumB"
                     /locus_tag="OO572_001271"
     CDS             8109..9755
                     /gene="fumB"
                     /locus_tag="OO572_001271"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6660337.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9898..11301
                     /gene="fumC"
                     /locus_tag="OO572_001272"
     CDS             9898..11301
                     /gene="fumC"
                     /locus_tag="OO572_001272"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6660338.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11298..12227)
                     /gene="tus"
                     /locus_tag="OO572_001273"
     CDS             complement(11298..12227)
                     /gene="tus"
                     /locus_tag="OO572_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6660339.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12303..13604)
                     /gene="rstB"
                     /locus_tag="OO572_001274"
     CDS             complement(12303..13604)
                     /gene="rstB"
                     /locus_tag="OO572_001274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6660340.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(13783..15102)
                     /locus_tag="OO572_001275"
     CDS             complement(13783..15102)
                     /locus_tag="OO572_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6660341.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15327..16070)
                     /gene="rstA"
                     /locus_tag="OO572_001276"
     CDS             complement(15327..16070)
                     /gene="rstA"
                     /locus_tag="OO572_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6660342.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16199..16534
                     /locus_tag="OO572_001277"
     CDS             16199..16534
                     /locus_tag="OO572_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6660343.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(16531..17253)
                     /gene="folM"
                     /locus_tag="OO572_001278"
     CDS             complement(16531..17253)
                     /gene="folM"
                     /locus_tag="OO572_001278"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6660344.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17290..18672)
                     /locus_tag="OO572_001279"
     CDS             complement(17290..18672)
                     /locus_tag="OO572_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6660345.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(18858..19802)
                     /locus_tag="OO572_001280"
     CDS             complement(18858..19802)
                     /locus_tag="OO572_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6660346.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20326..21858
                     /gene="pntA"
                     /locus_tag="OO572_001281"
     CDS             20326..21858
                     /gene="pntA"
                     /locus_tag="OO572_001281"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6660347.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21869..23257
                     /gene="pntB"
                     /locus_tag="OO572_001282"
     CDS             21869..23257
                     /gene="pntB"
                     /locus_tag="OO572_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6660348.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23282..24316)
                     /gene="tqsA"
                     /locus_tag="OO572_001283"
     CDS             complement(23282..24316)
                     /gene="tqsA"
                     /locus_tag="OO572_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6660349.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24727..25092
                     /gene="mdtJ"
                     /locus_tag="OO572_001284"
     CDS             24727..25092
                     /gene="mdtJ"
                     /locus_tag="OO572_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6660350.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25079..25408
                     /gene="mdtI"
                     /locus_tag="OO572_001285"
     CDS             25079..25408
                     /gene="mdtI"
                     /locus_tag="OO572_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6660351.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25447..26268)
                     /locus_tag="OO572_001286"
     CDS             complement(25447..26268)
                     /locus_tag="OO572_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6660352.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26368..26451)
                     /locus_tag="OO572_001287"
     CDS             complement(26368..26451)
                     /locus_tag="OO572_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660353.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(26544..26879)
                     /locus_tag="OO572_001288"
     CDS             complement(26544..26879)
                     /locus_tag="OO572_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6660354.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27276..28529)
                     /locus_tag="OO572_001289"
                     /pseudo
     CDS             complement(27276..28529)
                     /locus_tag="OO572_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            28636..29547
                     /locus_tag="OO572_001290"
     CDS             28636..29547
                     /locus_tag="OO572_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6660355.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            29682..30902
                     /gene="mlc"
                     /locus_tag="OO572_001291"
                     /gene_synonym="dgsA"
     CDS             29682..30902
                     /gene="mlc"
                     /locus_tag="OO572_001291"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6660356.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31027..31722
                     /gene="bioD"
                     /locus_tag="OO572_001292"
     CDS             31027..31722
                     /gene="bioD"
                     /locus_tag="OO572_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6660357.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(31675..32967)
                     /gene="clcB"
                     /locus_tag="OO572_001293"
     CDS             complement(31675..32967)
                     /gene="clcB"
                     /locus_tag="OO572_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6660358.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33126..33740)
                     /gene="dmsD"
                     /locus_tag="OO572_001294"
     CDS             complement(33126..33740)
                     /gene="dmsD"
                     /locus_tag="OO572_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6660359.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(33783..34637)
                     /locus_tag="OO572_001295"
     CDS             complement(33783..34637)
                     /locus_tag="OO572_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6660360.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(34639..35256)
                     /gene="dmsB"
                     /locus_tag="OO572_001296"
     CDS             complement(34639..35256)
                     /gene="dmsB"
                     /locus_tag="OO572_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6660361.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35267..37305)
                     /locus_tag="OO572_001297"
                     /pseudo
     CDS             complement(35267..37305)
                     /locus_tag="OO572_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37366..38475)
                     /locus_tag="OO572_001298"
     CDS             complement(37366..38475)
                     /locus_tag="OO572_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6660362.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9859 a         9420 c         9948 g         9622 t
ORIGIN      
        1 tcttgcgcca ttagtcctgg gacgctctat caccatttca tctccaaaga agccttgatt
       61 caggcgatta tcttacagga ccaggagagg gcgctggctc gtttccggga accgattgaa
      121 gggattcatt ttgttgacta tatggtcgag tctattgtct ctctcaccca tgaagccttt
      181 gggcaacggg cgctggtggt cgaaattatg gcggaaggga tgcgtaaccc acaggtcgcc
      241 gccatgctta aaaataagca tatgacgatc actgaatttg ttgcccagcg gatgcgtgat
      301 gcccagcaaa aaggcgagat aagcccagac atcaacacgg caatgacttc acgtttactg
      361 ctggatctga cctacggtgt actggccgat atcgaagcgg aagacctggc gcgtgaagcg
      421 tcgtttgctc agggattacg cgcgatgatt ggcggtatat taaccgcatc ctgatcctct
      481 catttcgcgg gctggtgata actgtgcccg cgtttcatat cgtaatttct ctgtgcaaaa
      541 attatcctcc ccgtgttcgg agaattcccc cccaaaatat tcaccgtagc catatgtcat
      601 gagagtttat cgttcccaac actctcgaac gaacgttcga ttgcttattt tatggcttct
      661 gtcaacgctg ttttaaagat taatgcgatc catatcacgc tgtgggtatt gcagtttttg
      721 gttttttgat cgcggtgtca gttcttttta tttccatttc tcttccatgg gtttctcaca
      781 gataactgtg tgcaacacag aattggttaa ctaatcagat taaaggttga ccagtatttt
      841 tatcttaatg aggagtccct tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa
      901 aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt
      961 tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag tgccaggcag ttttaacgat
     1021 cagttcgccg atgcagatat tcgtaattat gtgggcaacg tctggtatca gcgcgaagtc
     1081 tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat
     1141 tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca
     1201 tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt
     1261 gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac
     1321 ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccgggat ccatcgcagc
     1381 gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc
     1441 gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatatcagc
     1501 gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac cagcgggact
     1561 ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc
     1621 gtcacagcca aaagccagac agagtgtgat atctacccgc tgcgcgtcgg catccggtca
     1681 gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt
     1741 ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg ataacgtgct gatggtgcac
     1801 gatcacgcat taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac
     1861 gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgca
     1921 gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa
     1981 ctgtacagcg aagaggctgt caacggggaa actcagcagg cgcacttaca ggcgattaaa
     2041 gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa
     2101 ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt
     2161 aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc
     2221 gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg ttggtatgtc
     2281 caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaactgct ggcctggcag
     2341 gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt agccggcctg
     2401 cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat
     2461 caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat
     2521 tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca attgacctac atgggcctcg
     2581 ggctttgcta cagcctggtg aatattcctt atggttcact tgctaccgcg atgacccaac
     2641 aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat tgccgcttca ttgacctttg
     2701 tatgcctggc atttctgata ggaccgagca ttaagaactc cagcccggaa gagatggtgt
     2761 cggtatacca tttctggacg attgtgctgg tgattgccgg aatggtgctt tacttcatct
     2821 gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc gcagccgtca ttgaagatca
     2881 gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt gcgctgtgtg
     2941 tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc tatgtgttaa
     3001 atgataccgg gctgttcact gtgctggtac tggtgcaaaa cctggttggt actgtggcat
     3061 cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa aaagaatacc ttcctgattg
     3121 gcgctttgct gggaacctgc ggttatctgc tattcttctg ggtttccgtc tggtcactgc
     3181 cggtggcgtt ggttgcgttg gccatcgctt caattggtca gggcgttact atgactgtga
     3241 tgtgggcact ggaagctgat accgtagaat acggtgaata cctgaccggc gtgcgaattg
     3301 aagggctcac ctattcacta ttctcattta cccgtaaatg cggtcaggca atcggaggtt
     3361 caattcctgc ctttattttg ggattaagcg gatatatcgc caatcaggtg taaacgccgg
     3421 aagttattat gggcatccgc acatcaattg ccttagtacc ttgcggattt atgctactgg
     3481 cattcgttat tatctggttt tatccgctca cggataaaaa attcaaagaa atcgtggttg
     3541 aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag cgatatcact aattaatatt
     3601 caataaaaat aatcagaaca tcaaaggtgc aactatgaga aaaatagtgg ccatggccgt
     3661 tatttgcctg acggctgcct ctggccttac ctctgcttac gcggcgcaac tggctgacga
     3721 tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc agggctgata agcccagtgc
     3781 tggcgcggga agagatattt acgcatgggt acagggagga ttgctcgatt tcaatagtgg
     3841 ttattattcc aatattgttg gcgttgaagg cggagcgtat tatgtttata aattaggtgc
     3901 tcgtgctgat atgagtaccc ggtggtatct tgatggtgat aaaagttttg gctttgccct
     3961 gggggcagta aaaataaaac ccagtgaaaa tagcctgctt aaattaggtc gcttcgggac
     4021 ggattatagt tatggtagct taccttatcg tattccgtta atggttggca gttcgcaacg
     4081 tacattaccg acagtttctg aaggagcatt aggttattgg gctttaacgc caaatattga
     4141 tctgtgggga atgtggcgtt cacgagtatt tttatggact gattcaacaa ccggtattcg
     4201 tgatgaaggg gtgtataaca gccagacggg aaaatacgat aaacatcgcg cacgttcttt
     4261 tttagccgcc agttggtatg atgataccag tcgctattct ctgggggcat cggtacagaa
     4321 agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg acccgaatta
     4381 tacgttgaaa ggggagttgc tcggctttta cgcgcagctc gaaggtttaa gtcgtaatac
     4441 cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg cgccgtgggg
     4501 aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg tggtggatac
     4561 cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa tgcagtcctg
     4621 gcaattaggt gccaactacc gtgtaactcc gcaatttacg ctgacatttg caccgattgt
     4681 gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt gaaggcgcgg gtatcttagg
     4741 tggtatgaac tatcgggtca gcgaagggcc gttacaaggg atgaatttct ttcttgctgc
     4801 cgataaaggg cgggaaaagc gcgatggcag tacgctgggc gatcgcctga attactggga
     4861 tgtgaaaatg agtattcagt atgactttat gctgaagtaa gaaataacgc cggagagaaa
     4921 aacctccggc gtttcagatt gttgacaaag tgcgcgtttt ttatgccgga tgcggcgtaa
     4981 acgccttatc cagcctacaa aaactcataa attcaaagtg ttgcaggaaa aggtaggcct
     5041 gataagcgta gcgcatcagg caatctctgg tttgttttca gatgaaaacg ccggagtgaa
     5101 aattctccgg cgttttggcc gtgaattact gctgcggaat tgccggtaca gccggaacgt
     5161 taagagctgg catcgcaaac atgccaacaa aatcttctaa cgacattttc tgcccattta
     5221 acgttatctg accgttagca tattgcaggc tggtggtgat ggtattgtcc tgcaaggtgg
     5281 tcagacggaa catctgcccc attgctgatg caccttcaac ttgctgtttc gccagttttt
     5341 tcgcttgatc ttcctgataa ccttccagct tcgctacctg agtcatcaac tcagttgcca
     5401 tatccaccgg aatggtcagt ttcgcatcca gagatttaac cgaacgatct acttcctgcg
     5461 ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt caggaacagc gacagattca
     5521 gcgcactttc accctgactg tttttccagc ttagcggcgc gatagtaatc accggatcgc
     5581 ctttcaacat cagcggcagg gcgctaaaga aggcttccgt cactttctcc tgataaagtt
     5641 cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg agtttgcgcg ttatattgct
     5701 ggctaaactg atgccaggct tcaccatcaa tctggccgac ttttaaagtc agcttgccgc
     5761 tgcccagatc ctgattctgt accttcaggc tgtttagcga gtaatccagt tggctattga
     5821 tcgttttacc gtcattgacc agatccgatt taccgctgat ctccatgcct tccagcagtg
     5881 ccagttcttt gccttccact gaaatggtca ttttttccag tgacagtttt tggtttccta
     5941 cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt tttcagatta ttaaaggtca
     6001 actgcacttt ctggttgtat tcgttaactg cgtctatccg accactttgc gcctctccgg
     6061 aaagggagat ggctttgccg tctctgtcag catttaactg gaactcgccg ccgctaaagg
     6121 cgactttttc atccttttgc tcgtaattca gtggattgag cgaaatatcg gaactggaat
     6181 caccgctgta accaatgcgc gagttaatct caaaaggcgt ttcacctttt gccatatcaa
     6241 acagtggttt gcttacttcg ttattaacca gcgtggtttg aattgatgcc atcgacggga
     6301 tcaggttcag ttttttaagc tgggcaagcg ggaagggacc atgatcaacc gattcgttga
     6361 agatgacgct ctgaccgctt ttaatccacg gattttcttt cccggcaatg ggtttcacca
     6421 acagttgcaa ctggctgctg aatacgccgc gatgatagtt ttgataactc acttccaggt
     6481 tggattcagg agctgtcagt ttgagttgcg cgttcgcctg cgcgaccatg tcttcgagat
     6541 gggtttcaat cttcttgcct gtataccatg cgccgcctgt ccagactacg cctagcgcaa
     6601 caatgacgcc taccgctacc agcgatttat tcataatgat tatccataaa atgaaatcag
     6661 gcggactggc cgcctgaagg tgttataagc ctttaataag cttagcaaga gatgttaatt
     6721 ttttcagtaa gctcttacag cttgttgtaa acacgcgcta aacggccgtg gcctttgaca
     6781 gtcaccggtg attcgttggc ggcaataaac gctgattcac ccggtttaag ctgtaactgc
     6841 tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga acaaaatggc ggcactctgc
     6901 tggctgatgg tggtttcttt atcactaagg tcatgcagcg agaaggcaaa atcatccaca
     6961 ggaatcggga agtccagttc tgcaccttgt ttcaccggct gggtcaacaa ctggttagcc
     7021 ggtttggctt cgaatttcac attggcaacc agttccggaa tatcaatgta tttaggcgtc
     7081 agacctgcac gcagcacgtt atcggagttc gccatcactt ccagcgccac gccttgcagg
     7141 taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc caggattcaa tttcaccaca
     7201 ttcagtaaca gcggggagaa caggccgctg tcttccgggt aaaattcaga aattaaacga
     7261 atcgtttgcc acggttcacc ctgctgactg tcgagcgccg attttaaaat cgccagcgcg
     7321 cgggattttt cttcaccctg catattcaac aggttggcga acagttcgct taaacgttcg
     7381 gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg caccggcgac cggctggagt
     7441 agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg caaggaaagg cgtcagcgca
     7501 aaaaccagct ccggcttgtg gttaggatct ttatagttac gctcggcggc atccatcggg
     7561 atacctgcgg cattttcttt ggcaaaaccg atttcagaat tgtgtttgtt tggatgaacc
     7621 tgaatggaga gtggctgtgc tgcgcataat actttgaaca ggaaaggcag ttcgccaaag
     7681 cgtttggcaa cggcctctcc gagcagagtc gatttatcac tctcaatcac atcacgcagt
     7741 gaaacgatat ctccggcggc attctgcact cgtgaactgc ttttcggatg tgcgcccatc
     7801 cacagctcgg ccatcggctg gctggacgga ttttccatac cataaagttc agtcaacgcc
     7861 gttttgctac cccaggcata gttttgcact gagttaatga gtttttgcat gatcaatccc
     7921 tgttttaatg tggaaattaa tcccgctatt aaagcaagaa tcctacggga agtaacctgg
     7981 agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa actgtgtagg atattgttac
     8041 tcgcttttaa cagggcaacg gaacacccgc ccagagcata accaaaccag gcagtaagtg
     8101 agagaacaat gtcaaacaaa ccctttcatt atcagtctcc ttttccactc aaaaaaaatg
     8161 atactgagta ttacctgcta accagtgaac acgttagcgt atctgaattt gaagggcagg
     8221 agattttgaa agtcgcaccc gaagcgttaa ctctgttggc gcgtcaggcg tttcatgatg
     8281 cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc cgacattctg cgtgacccgg
     8341 aggccagcga aaatgataaa tatgtggcgc tgcaattcct gcgtaactcc gacatcgcgg
     8401 cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc gattattgtt ggtaaaaaag
     8461 ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct ggcgcgcggt gtctataaca
     8521 cttatatcga agataatctg cgctactcgc aaaacgcgcc gctggatatg tataaagaag
     8581 tgaataccgg caccaatctg ccagcgcaga tcgatcttta tgccgttgat ggcgacgaat
     8641 acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa caagacgtat ctctatcagg
     8701 aaaccaaagc gttactgacg ccggggaaac tgaaaaatta cctggttgag aagatgcgca
     8761 cgctgggtac cgcggcctgt cctccgtatc atattgcgtt cgttattggt ggaacttctg
     8821 cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa atactatgat gaactgccaa
     8881 cggaagggaa tgagcacggt caggcgttcc gcgatgtgga actggaaaaa gaattactga
     8941 tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg taaatacttc gctcacgaca
     9001 tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc ggttggtatg ggtgtttcct
     9061 gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca ggggatctgg atcgaaaaac
     9121 tggaacataa tccaggtaaa tatatccctg aagagctgcg taaagcggga gaaggcgaag
     9181 cggtgcgcgt tgaccttaac cgtccgatga aagagatcct cgcacagttg tcgcagtatc
     9241 ccgtttctac acgcttatcg cttaacggca cgattatcgt tggtcgtgat attgctcacg
     9301 ccaaactgaa agagcggatg gataacggtg aagggctgcc gcaatacatt aaagatcatc
     9361 cgatttacta cgcgggtccg gccaaaacgc cggaaggtta tgcctccggt tctcttggcc
     9421 caacgaccgc cggacggatg gattcttatg tcgatcaact gcaagcgcag ggcggaagta
     9481 tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac ggatgcctgt aaaaaacacg
     9541 gcggcttcta ccttggcagt atcggtggtc cggccgctgt attggcgcag ggaagtatta
     9601 agagcctgga atgtgttgaa tatccggaac tgggaatgga agccatctgg aaaattgaag
     9661 tggaagattt cccggcgttt atccttgtgg atgataaagg aaatgacttc ttccagcaga
     9721 tacaactcac acagtgcact cgctgtgtga aataaacaga gccgcccttc ggggcggttt
     9781 ttttacatgg cacgaaagac caaacatttg ttatcaaata gtaaataatg agtgagctaa
     9841 aagttgctta acgaaagcaa aacagaaaga aaaaattaat caggtgagga gcaggccatg
     9901 aatacagtac gcagcgaaaa agattcgatg ggggcgattg atgtcccggc agataagctg
     9961 tggggcgcac aaactcaacg ctcgctggag catttccgca tttcgacgga gaaaatgccc
    10021 acctcactga ttcatgcgct ggcgctaacc aagcgcgcag cggcaaaagt taatgaagat
    10081 ttagacttgt tgtctgaaga gaaagcgagc gccattcggc aggcggcgga tgaagtactg
    10141 gcaggacagc atgacgacga attcccgctg gctatctggc agaccggctc cggcacgcaa
    10201 agtaacatga acatgaacga agtgctggct aaccgggcca gtgaattact cggcggtgtg
    10261 cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga acaaaagcca aagttccaac
    10321 gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg cactgcgcaa gcaactcatt
    10381 ccgcagctta aaaccctgac acagacgctg agtgaaaaat cgcgtgcatt tgccgatatc
    10441 gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc taacactagg gcaggagatt
    10501 tccggctggg tagcgatgct cgagcataat ctcaaacata tcgaatacag cctgcctcac
    10561 gtagcggaac tggctcttgg cggtacagcg gtgggtactg gactaaatac ccatccggag
    10621 tatgcgcgtc gcgtagcaga tgaactggca gtcattacct gtgcaccgtt tgttaccgcg
    10681 ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg ttcaggcgca cggcgcgttg
    10741 aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg tccgctggct ggcctctggc
    10801 ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg agccgggcag ctcaatcatg
    10861 ccggggaaag tgaacccaac ccagtgtgaa gcattaacca tgctctgctg tcaggtaatg
    10921 gggaacgacg tggcgatcaa catgggtggc gcttccggta actttgaact gaacgtcttc
    10981 cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct tgctggcaga tggcatggaa
    11041 agttttaaca aacactgcgc agtgggtatt gaaccgaatc gtgagcgaat caatcaatta
    11101 ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc acattggtta tgacaaagcc
    11161 gcggagatcg ccaaaaaagc gcataaagaa gggctgacct taaaagctgc ggcccttgcg
    11221 ctggggtatc ttagcgaagc cgagtttgac agctgggtac ggccagaaca gatggtcggc
    11281 agtatgaaag ccgggcgtta atctgcaaca tacaggtgca gccgtggaat gatcaaacga
    11341 agcggctgcg cctgaggttt ataacggtgc tgcacattgt cggcatcgta atttaacaac
    11401 tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa tcagtgcaat cagtggtgta
    11461 gggcaggcgt gttggacttg tttttgatct cctttgtacc agacgcgggc aatcggctgc
    11521 accttcactg gacgtttgat ttttaacttc gcgttctgtg gcagggcagc gatatcctga
    11581 tactctcgct ccagttttct ttgccactcc tcgcgcgtcc acggtgcgac actgcgtggt
    11641 gatttcaggc ttttttccag ctgtgccagg acttcatcac gatgcaaatt cttaatgata
    11701 tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt gcagaacggt gagcgtgcgg
    11761 taagcattaa gggtgatcag ccccggcaaa tgacgatgca cccattcaaa acgtgccgcg
    11821 gtggggagtt ctgattcaac cgtgacgata tgctcgaacg tggtcttgag tttattgatg
    11881 tgctgaatat gactgaccaa cgctgcttgc gaaaggttat cgacctggta acacaacacg
    11941 ccaggcagac ggacagcggc cttgctgctg cgattttcgg actgttgttg aataaataaa
    12001 tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc cgagatgttg ttttacctca
    12061 atacgattaa gcggattatg ctcatcctct ttttttacct ccggcaaaga gaacacgcgg
    12121 gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg tagccagctc ttgttccatc
    12181 tggcgaaagg tagtgttgag tcggtctacg agatcgtaac gcgccataat attaaccact
    12241 ttagttacaa catacttatt ttatagcaca gtcgtggtga cttgaccatc gtgcgcgtag
    12301 tgtcaggcag aggtaaattg cgggatgtta tgccataacg gccagctaaa cgagaagcga
    12361 gcaccaccca gttcgctgat gtcacaatta accgtaccgc ccattgtcag tgctattgag
    12421 tggacaattg ccagccccag cccgcagccg ccggttgagc gatcccggct gggatcgagg
    12481 cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg caatccctgg gccatcatcc
    12541 tcaacaatta atgtcgctct attccccgac agtagcaggc tggtttcaac cgttgaatgg
    12601 cagtagcgca gggcgttatt gagcaaatta tccagcacgc gctccattaa gcgcatatcc
    12661 aacgccgcat aatggccttg catgagcgtt tttatccgta ccgttttatc gggcgttact
    12721 gcctgaatat ctgccagatg cgttgacagc cacaacggca ggtctggttc gctaagatga
    12781 agctcgtttt gtgggcgatc gagtcgggca taagtcagca gctcttcaat taaagcttca
    12841 agttgactga tatcacgatt caacgcctgg gattcggcgg cgctcaggtt atcgctcatc
    12901 tccagtcgat aacgcaggcg cactaacggt gttcgcagtt cgtgagcgat accgtcaata
    12961 agctgttttt tgctggcaat taaggcgttg atattgtccg ccatctggtt aaatgcgata
    13021 ccaagtcgtt caaagctcga accctcatca aagtggatac gttcattgag atgcccatcg
    13081 ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct gccagtgcgg acgcatccag
    13141 ataaacaccg gaaaggcgag ggaaatagca ataaaagcga tcagggcgat atccagcaat
    13201 cgcatttgat ggaggtaata aagataagga acaggaccaa ctgccagcac gtagtggctg
    13261 cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg cgacaatttc gccgccacgc
    13321 agtcggtgca tggaaatatc atcaagatgg tatttactca gtggctcgac acgcagatcg
    13381 aaagagagat ttaaatccat ctctttcagc gtcttacccc agtcgtgtgg ggggatctct
    13441 cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca aatcatccag cgactgtttg
    13501 cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca gcagagacat cacaaggaag
    13561 cagacaaaca ataacaggta aaactggata aacaattttt tcattggcat cgcccgcaac
    13621 agaatactat tcgtattaat tcacgactgt ggagcaatgt aaaccgccaa ctggataaga
    13681 ccttgtttgg aagataatgt ccggaacaga cggatgtatc cccggtgtat tcatattcag
    13741 taaattcaat tttccaccgg ggcttgttta ttatgctaag gtttaattta acgtgacgct
    13801 ttgttgttct ttagtctctc tatccttaag aagacgggct gatattacac cggacattat
    13861 tgaaccattc acattaagcg ctgttcttgc catatcaacg atcggctcta tggcaataaa
    13921 aataactact aatgcgacag ggaaattaag tgttgaaagg acaatcagcg ccgcgaatgt
    13981 cccaccacta cctacgcctg ctacccctat tgagccaagc gcgatggcag gtaaaagagt
    14041 tgcgaggaat tgcaatgaga agggatcgat acccattgtt ggagcaatca tcgccaccat
    14101 cacggcagga taaatcccag cgcagccatt ttgtcccatg ctggaaccaa aagacgctgc
    14161 aatattagca atcgtatttg gaacccccaa tttttccaat gcagaaatag ccagaggaat
    14221 tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt acttgccaga cggtcttgta
    14281 atagttaacg gggtttttac ctattaacag caaaatgccc gagtgaacaa taaacatcac
    14341 aaaaacggcc agataacagg cgacgatgaa cccgagcaaa ttggtaaact gtgaaagctg
    14401 atacgttgag aagactttcg ccattagtgc cattacccca taaggcgtca gcgcaatgac
    14461 aatacggacc attttcatta cccatatttg aatcgcgttg atccccgcgc tcaatttttc
    14521 gccctctgct ggtgcatctt tctttacttt taacagcgca ataccagcaa gaattgtaaa
    14581 gatgacgatg ccaataaccg aaacacttct tgccccggtc agatcaagga agaggttggt
    14641 tgggatcaat gacgtaatga gtcgcggtaa agataccgca gctgttttcg agacgtcatc
    14701 gctggttaaa gaggattgca tatgaccaaa ggcgctggca tcaaggccaa ttgcactggc
    14761 aatagccagg ccaactaaac ctgcaatcat gactaaacac aacatacagg caacaatagt
    14821 taaagacatc ttgccaatac cagcgctatt ttcgagctta ttaatcgctg aaagaatcga
    14881 aatgaatatg agtggaacgg caacaagttt taataaatta acataaccgt taccagcaat
    14941 attataccat tcgataacgg tttcactgct tgtctcagga atgaaatgca gggagatacc
    15001 aatgatagcc cccaggaaaa tcgcgataaa aacacttttg gaaagggatt cgctcttttg
    15061 atccgttttg aaaatccccc atgagcagat caaaaaaagc acaatatatg tgaaagttgt
    15121 aaccgttttt taagcctctt gcctcattca gcacctgaaa tgccggaaag agtttgttgt
    15181 aaaaaatctg cgccataaga cgcagggacg aggcgattat agagaaattt tttctcattt
    15241 tcatatcaaa tgtagggggt ttggaatttg aataagctct tattaattga attcgttaat
    15301 tgttctcttt ttcagatgaa caaatattag tcatctgatt gtttattgtc actatcccat
    15361 gcatgaggcg caaaaagata gcctttgtta cgcacagttt taatgcgata aggttctgcg
    15421 gcgttatcga gcagtttttt tcttaaccgc gaaatagcca cgtccacgct acgatccagt
    15481 ccgtcataac tgacgccgcg taaatttttc agcaatgcat cgcggtccat gatttgcccg
    15541 gcatgcgtag ctagttccca caataattcg aaatcagctg tcgagagcga gatttcagtg
    15601 ttagccaggg tgactacgcg gttgatgggg tcgatagtta acgtgccgaa atgcagggct
    15661 ttgtagggag taagagacgt ttcctgaaga cctttggtca gtgtggcttg ctcattctga
    15721 cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg gcgtcgtttt gagaatatag
    15781 tcgcaggcac ccatttccag tgccaggatg tggttcatat cgctatcgag agaggttaga
    15841 agaacaatcg gtccagacca ctttgcgcgt aaatcacgac aaatggtcat gccgtccttg
    15901 cctggtagca tgatgtcgag taacaccaaa tccggatttt ctcgcaaaat ggtttcttcg
    15961 gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat gttttgccag gtacgcggca
    16021 atcagtgaac cgacttccgc atcatcttcc acaaatacga tagtgttcat aacattcacg
    16081 gtagatataa aaacgtcaca atacaccgcg ctgtttttac ttactatcaa tcgtttctaa
    16141 acaagttttc atcaggtatt ctactgatga cttgttatgg aagtgttaag gtaaaaagat
    16201 ggggctggta atcaaagccg cgctcggtgc gctggtagtg ctgttgattg gtgttttagc
    16261 aaaaacgaaa aattattata tcgccgggct gattccactt ttcccgacct ttgcgcttat
    16321 cgcgcattat attgttgcca gcgaacgcgg cattgaagcc ttacgcgcaa ccatcatttt
    16381 tagtatgtgg tcgattattc cctattttgt ttacctggtg tcgctgtggt atttcaccgg
    16441 aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg tgttggggga taagcgcatg
    16501 ggtgttgatt atatgctgga taaagctgca ttaacgcaga tgacgaccgc catcaagtgg
    16561 gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa tcgaccaggt cgatcacttc
    16621 tttctcgcca ggcgcggttt tcatcagtga tttattcagc gcctgttgtc gatattcggc
    16681 atcatcatgt tcattaaaca ggatcagcga tggcgcaata gaattcactt tcacttccgg
    16741 tgccagcttg cgggcaaacg agcgggtcat attatccagt gccgctttgc ttgcagcata
    16801 cgcaacatgt ttgtcgctac cgcgctccac cacataatcg gtaaagtgaa tgatatcgct
    16861 ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca tggttgagca ggtatggggt
    16921 attaacgtgg atctgcatca tgcaagccag tacgtcggcc agtggcgcac ccggtttttc
    16981 cgcaatccac gcactggcgt tatgcaaaat agcacgcaga ccatgggtgg tttttagtac
    17041 ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa tcagcctgaa tacactgcgc
    17101 acctgcatta atcagtccat caatggctgg atagtgtgtc cgatagctga caatcaccgg
    17161 ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg atgcgacgac ctccgccagt
    17221 aattaatatt ggcaagggct gggttttacc catcgttatc tcctttgcta tccaacgatg
    17281 ggatgaacgt tatcccacca gcatccaggt cgccggaact gaggcaatta acagcatgcc
    17341 aatcagcacc atttcctgac ggttcagtac attatcatgt gtatgcgttt tgcgcgcata
    17401 caggaaaacc agcagccccg gtgcatacag aacaacggat aacagcaagt gcattggacc
    17461 agaggcgtac aataaccata agccataaat gcaggcaccg acacctaccg ctttatgcag
    17521 tggacgggtt gcgattttca gcaagaacgc gccgaccagg aaatagggca ccagaatcat
    17581 ttctgaggcg atggtcagca acgtgttgta atcggaaccg gtcagccaga tgagtaccag
    17641 acaaatttgc acgcagatgt tggtcaacca cagtgaggcg gatggcgcag cttgtgcgtt
    17701 ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca gccaggaacg gaacttctgc
    17761 cgccatgatg gtccagctta agtacgcgcc gcaaacggaa acgatcaaac cggcagcgat
    17821 gatgatttcg ccccatggtc ccatcatttc caccatcaga ccggccattg acgggttacg
    17881 aatttcagcc agttcaggac gtgccaccac acccagcgaa agcagcgtta ccagcaagta
    17941 aacgcccaga gcggagagaa ccgccagcag tgtcgctttg ccaacatcac gtttattacg
    18001 cgcacgcgca gaaacaacca cggcaccttc cacaccaatg aaaacccaca gggtgatcag
    18061 catggtgttt ttcacctgtt cccaaacagg tacgccaagt gcaagtccgg tgaagtcgag
    18121 cttgaaggta tccagtttga acatcatcat cgccagcaca acaaacagac ccagcggcaa
    18181 caattttgcc agtgtcgcca ccaggttaat gctggcagcg gtttgcaccc cgcgcaggat
    18241 caaaaagtga acaatccaca ataaagccga tgcaccgact atcgattgcc aggtattacc
    18301 atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag cttaacgcgg aaaaaacgat
    18361 caccagatag gagacgttgg cgatgaccgc gcacagccag tatccccatg cggaacaaaa
    18421 gccgattagc tcgccaaacc cttcgcgggc ataggtaaag ataccgccat caagttcggg
    18481 acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat aaaatgccag cgccagtaat
    18541 accccagccg atgagcagtg ctgccgggct ggcaactgcc gccatatttt gcggcagact
    18601 gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg gtgagtgcgc tcagtcccag
    18661 tttcttttcc atcgatatcc agtgattaaa acgtgataag aagaataaat attagggcaa
    18721 aacgcataaa aatggagttt gccactgaag cgcgggattt tacgaagaga tgtgtctgca
    18781 tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa ggcggcatca agccgcctta
    18841 taggagtaac tgaatagtta tttatacaga tctgcgctga cggtcaggtt attaccacgt
    18901 ttctgccact ggcgggtgat gtggtaatac ttagcacctt tcttcgcggc acgtttcgca
    18961 acctgatagg agacttcggt catgttgccg tagttgccag agaatttgat gctgtcgaac
    19021 gggaccatca tcgctgctgt cgctttgttc agttcttcga ctttagtgcc atccgggagc
    19081 gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa agaagcgacc gacttcagaa
    19141 cttggtgatg cggtagtcgc aacacccggg atctcaactt tcttcgcggc ttcgccaccg
    19201 gcagccagag ctgcacgtcc tgcttcggaa tctgccggga tcacatccgg gctctggacg
    19261 atacgtttct tagcatcttt tttatagatg aatgcagtaa tacgctggtt gccgccctgg
    19321 ttggcatcga tttgacgaac gatgtagaaa gagtaggcac ctttcgcttt tgccgctttg
    19381 gtgatggcat cattgacttc tggctggcta cgatagaagc cctggacggt gaccgtgtca
    19441 aacggttcaa tcagaacagc ctgatctttc ggcagttcga caacaccgtt aattacgcga
    19501 ttacttgttt cttctgcttt ttcagcatcg gctttataga ggtcagcgac cacacgccag
    19561 ttaccgctgt taccaaaatc agaagtgtcg acaacataaa aagaggcggc accttcttta
    19621 tctgcgcgac gagaaacggc tttcaccgct tcgccaatag cattaaaacg accggtaacc
    19681 actacacggt caaaaggttt aaccgctgcc gcttgctccg gtgtcagttc tgttgctgcg
    19741 ttaacggaga atgccgtagc agaaagcagt gccgacgcca ggagggtgtt cttaagcttc
    19801 ataaaaataa tccttcgcct tgcgcaaacc aggtactggt attgttatta acgagaaacg
    19861 tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc atttttcata tcacattcct
    19921 taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt aaaaaacagc cgtatcagca
    19981 tcattactac tgaagcaact gaattgtata agttaattta atgttaagta gtgattcgtg
    20041 ccggggcgat gtctcgtttt acccgaccgt cgaagacaat tatcagtctt tatccggcgt
    20101 tctaaggtgt ttatcccact atcacggctg aatcgttaat attttgcgag ttcacgccga
    20161 aatactgatt tttggcgcta gatcacaggc ataattttca gtacgttata gggcgtttgt
    20221 tactaattta ttttaacgga gtaacattta gctcgtacat gagcagcttg tgtggctcct
    20281 gacacaggca aaccatcatc aataaaaccg atggaaggga atatcatgcg aattggcata
    20341 ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa cgccaaaaac agtggaacag
    20401 ctgctgaaac tgggttttac cgtcgcggta gagagcggcg cgggtcaact ggcaagtttt
    20461 gacgataaag cgtttgtgca agcgggcgct gaaattgtag aagggaatag cgtctggcag
    20521 tcagagatca ttctgaaggt caatgcgccg ttagatgatg aaattgcgtt actgaatccc
    20581 gggacaacgc tggtgagttt tatctggcct gcgcagaatc cggaattaat gcaaaaactt
    20641 gcggaacgta acgtgaccgt gatggcgatg gactctgtgc cgcgtatctc acgcgcacaa
    20701 tcgctggacg cactaagctc gatggcgaat atcgccggtt atcgcgccat tgttgaagcg
    20761 gcacatgaat ttgggcgctt ctttaccggg caaatcactg cggccgggaa agtgccaccg
    20821 gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg ccgccattgg cgcagcaaac
    20881 agtctcggcg cgattgtgcg tgcattcgac acccgcccgg aagtgaaaga acaagttcaa
    20941 agtatgggcg cggaattcct cgagctggat tttaaagagg aagcgggcag cggcgatggc
    21001 tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa tggaactctt tgccgcccag
    21061 gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc caggcaaacc agcgccgaag
    21121 ctaattaccc gtgaaatggt tgactccatg aaggcgggca gtgtgattgt cgacctggca
    21181 gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg aaatcttcag tacggaaaat
    21241 ggtgtcaaag tgattggtta taccgatctt ccgggccgtc tgccgacgca atcctcacag
    21301 ctttacggta ctaacctcgt taatctgctg aaactgttgt gcaaagagaa agacggcaat
    21361 atcactgttg attttgatga tgtggtgatt cgcggcgtga ccgtgatccg tgcgggcgaa
    21421 attacctggc cggcaccgcc gattcaggta tcagctcagc cgcaggcggc acaaaaagcg
    21481 gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac cgtggcgtaa atacgcgttg
    21541 atggcgctgg caatcatcct gttcggttgg ctgtcaagcg ttgcgccgaa agagtttctt
    21601 gggcacttca ccgttttcgc gctggcctgc gttgtcggtt attacgtggt gtggaatgta
    21661 tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg cgatttcagg gattattgtt
    21721 gtcggagcac tgttgcagat tggccaggga ggctgggtta gcttccttag ttttatcgcg
    21781 gtgcttatag ccagcattaa tattttcggt ggcttcaccg tgactcagcg catgctgaaa
    21841 atgttccgca aaaattaagg ggtaacatat gtctggagga ttagttacag ctgcatacat
    21901 tgttgccgcg atcctgttta tcttcagtct ggccggtctt tcgaaacatg aaacgtctcg
    21961 ccagggtaac aacttcggta tcgccgggat ggcgattgcg ttaatcgcaa ccatttttgg
    22021 accggatacg ggtaatgttg gctggatctt gctggcgatg gtcattggtg gggcaattgg
    22081 tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca gaactggtgg cgatcctgca
    22141 cagcttcgtg ggtctggcgg cagtgctggt tggctttaac agttatctgc atcatgacgc
    22201 gggaatggca ccgattctgg tcaatattca cctgacggaa gtgttcctcg gtatcttcat
    22261 cggggcggta acgttcacgg gctcggtggt ggcgttcggc aaactgtgtg gcaagatttc
    22321 gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac ctggcggctc tggtcgtttc
    22381 cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc ggcctgcaag tgctggcatt
    22441 gctgataatg accgccattg cgctggtatt cggctggcat ttagtcgcct ccatcggtgg
    22501 tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac tccggctggg cggctgcggc
    22561 tgcgggcttt atgctcagca acgacctgct gattgtgacc ggtgcgctgg tcggttcttc
    22621 gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac cgttccttta tcagcgttat
    22681 tgcgggtggt ttcggcaccg acggctcttc tactggcgat gatcaggaag tgggtgagca
    22741 ccgcgaaatc accgcagaag agacagcgga actgctgaaa aactcccatt cagtgatcat
    22801 tactccgggg tacggcatgg cagtcgcgca ggcgcaatat cctgtcgctg aaattaccga
    22861 gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc cacccggttg cggggcgttt
    22921 gcctggacat atgaacgtat tgctggctga agcaaaagta ccgtatgaca tcgtgctgga
    22981 aatggacgag atcaacgatg actttgctga taccgatacc gtactggtga ttggtgctaa
    23041 cgatacggtt aacccggcgg cgcaggatga tccgaagagt ccgattgctg gtatgcctgt
    23101 gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa cgttcgatga acactggcta
    23161 tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc cacatgctgt ttggtgacgc
    23221 caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc tgacggcctc tgcggaggcc
    23281 gtcactcttt attgagatcg cttaacagaa cggcgatgct ttgacctccc gctgtttgtt
    23341 caagcgcaat tttgacaata attgtcaacg gcacggaaag cagcataccc accggtccta
    23401 acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa tgtcgaaagc cccagcccac
    23461 gtcccatgat gcgcggctcc agaatattgc cgaagaccag attaatcagc agatatcccg
    23521 ccagcaccag caacgcttcg tagaagccat taaacaccag tacctgagcg atagggggga
    23581 ttgccgcgag aactgaacca atattaggga tgtaattaag cgcaaaggcc agcaatcccc
    23641 agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc ccaggcgacc aggccggtga
    23701 tgatgctgat ggctgttttc agcaccagat aatgagaaac actgtcaatc gcacgttgaa
    23761 tcgccgccat cccttcaacc ggacgcgcca tcatttgctg aaattttccg ggcaattgtg
    23821 gcacttcgag cagcataaac agcaccgtca gcagcaataa aaatattgat gacatggcat
    23881 tagataactg cgtcaataag ttggtgagca acgtcatcgc cgcgttcgga tcaatgtaat
    23941 gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg caacaacggc tcaagagctt
    24001 gcagcggcgt cataatagag ttgcgatatt gcggtaacgt ccgcgtcaac tcgttgagcg
    24061 cggaacccag ataagctaat agcaacacca tcgccatcac gatgattgtc atcaaaatcg
    24121 acactgccag tacacgcggc acacgccagc ggaccatatg ttgcaccagc gggttaagaa
    24181 taacagcaat aaataatgcg agaataaacg gcacgatgat ctcggcggca aaacggatac
    24241 cgcagagaat aatgaccagc attcccaaca taatgacgat ttttaggcca ttgagcgtga
    24301 tgatcggctt tgccatgctt gtccccgttt ttcttccttt gctaataata atgtcttttt
    24361 cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa gaagtgaaaa tattttgagt
    24421 taattcttaa gctatgatac aaatcaggcg tgttcaacta ccgaggacaa ttatcatctg
    24481 cgatgacgag aagcaacact gcggataatt gtaatattat ggacaatttg ttcaggacgt
    24541 tattttctac atttacccat cttcgtacct catccactat cttgctcgta ggtgagcagc
    24601 actggcgcaa cgcgctttag tcctttcttc tgcctaagct tgccctatgc gcagggcttg
    24661 accactttac tttggtttcg ctgaattaag cgaaaattaa aatagttctc ttgcaggaga
    24721 aggacaatgt atatttattg gattttatta ggtctggcta ttgctacaga aattaccggt
    24781 acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg gcggctttat tttaatgctg
    24841 gtgatgattt ctctgtcgta tatatttctc tctttcgccg ttaaaaaaat cgccttaggc
    24901 gtagcttatg cgctgtggga aggtatcggt attttattta ttaccttgtt tagcgttttg
    24961 ttattcgacg aaagtttatc gctgatgaaa attgccgggt taaccaccct ggtcgccggg
    25021 atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac ctgaactgga ggtgaaccat
    25081 ggcgcagttt gaatgggttc acgccgcctg gctggcattg gcaatcgtgc tggaaatcgt
    25141 tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc aaaatatttg gcttgctctc
    25201 cctggcggcg gtgctggcgg cctttagtgc gctttctcag gccgttaaag ggatcgactt
    25261 gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc gccacgttag ccgcaggttg
    25321 gatcttgttt ggtcaacggt taaatcgtaa aggctggatt ggcctggtct tgctgttggc
    25381 tggaatgatc atggtgaaac ttgcctgatg aagacgctgc ccgcgctgtc gggcagcgtt
    25441 tgaacattat ttttgcgaca gttgatccag cttgtcgcga aaaccggtaa cagaaatggc
    25501 ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc gaactttgca ccccaattaa
    25561 ttgccagccg tcatcggtat gcaacatcag aggcgaaccg ctgtcacccg gcaaggtatc
    25621 gcactgatgt gacatcaccg acgtttgcgc ccagccagtc acttcacagt tttgatgact
    25681 gtataacgta tcgagatgat cttcagggta tcccgcctga gtcactttac gaccagccga
    25741 ttttaatgcg gcagtaagcg cggctttatc tccctcaaat aacggcaacg gtgtaatgcc
    25801 agaaggggga ttacgtagca caatcaatcc gaagtcccac ggtgcagctg cgggaggaac
    25861 aatccaacca tccccatctg cttttaaccg ctttcccagt gtcggatcaa cgcggccttc
    25921 tatgtcgtgg atctcatagc gccaaagacc tttatttgac acaaaacgca gcgctaccgc
    25981 cttatccgct ttacccttag gcggcgtcaa taaacagtgt cctgccgtta atgccagatt
    26041 gggtgcaatc agcgtcgccg tacataaatt gccgctggcc gtttccagtt gcccaaccgc
    26101 atcccacggt gattgggtcg tgtcattcac tggcacacga tcatcatgac caaaaaacag
    26161 ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt ttatctgcaa acacaaaagc
    26221 agacgtcaaa ctaattgcac ccaacactac agcaatggtt gtacgcatat cacactctgg
    26281 tgggtaatta tgattattaa aagcaaaccc tcataaatac tatagacggg acgggatgaa
    26341 agtgggagta aaatcagata actacagtca ggaaagataa aaacgggcgg caatcagtgc
    26401 gcataaaata aggatgataa gaatgaactc aaagcgataa cggcccacca tactaccctc
    26461 cgctaaaggc ggtgctaaac gttacccgct tagcaccgta attttcaggc gcgagggggc
    26521 gcgccagcat tactgttgaa aacttacgct gcgggttgtg cagcaggttt tgccggttgc
    26581 tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg ctttttgttc aggggctttc
    26641 tgttcagctt tcgtattttt atgatgcttt ttagccgcct gcgctttctg ggcaggtgct
    26701 gctttatgct gttttttatg atgtgtagtt ttcgctggcg ctgctttggt ggtcgtcgca
    26761 gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg cagaagacag acccatagca
    26821 gcggcaacaa ccagagctaa tacttttttc attgtcatac cctcaatttg ttttttcatt
    26881 taaccccact gcggggccgt tgaaataact atatccctgg gaatggcaga cttccgtgag
    26941 tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa taaattacag cgttgataat
    27001 aacgtattgt gacttaagaa aaatttagct accaataata gtagtcttga tcgggttaac
    27061 tattatacta cttacaaagc ggtagaataa ctgcgcatca gtaataaatg acacacagca
    27121 aaatgaatcc gtttatttgg gtacttataa tcctgatgac gctagatgcg ctgcgggaat
    27181 tggctggcgc ttcgtctatt ttaggatggc tattaacgtt ggtttgagca ggcaataagt
    27241 ccggacgggt atttaccgca gtccggactt atttttcagg cgtgcagacg acgatgcaaa
    27301 cgcgtcccga ccagcagagc aatgaccagc atcagagcaa taaatgcgcc gacgccgttc
    27361 cagccatagt tatgccagaa aacaccaccc agcgtcccgg caatactcga ccccagatag
    27421 taactgaaca gatacagcga ggaggcctgg cctttagcgc gttttgcgcg ggggccgatc
    27481 cagctgctgg ctactgagtg ggctgcgaag aatccggctg agaagagtaa cattccggca
    27541 aagatcagcc acagcgagct aaataaggtc atcagtaaac caaacggcat aactcccgtc
    27601 gaaaacaaca tcaccggacc gcgcccatag cgggtggtca tggttccggc tttgggtgag
    27661 ctccatgtac cggtcaaata agccagcgat aataagccaa ccacggcctg actgacatgc
    27721 tagggggaaa gcatcaaccg atagccgatg taattaaaca gcgtgacgaa cgaccccatc
    27781 agcaaaaagc cttctgcgaa caataacggt aatccccggt cacgccagtg cagacgaaag
    27841 ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa aatggcgtga ttcagggagg
    27901 attttccaga acatcaacgc cgaggccagc gcgaaacaac cgattgccgc cagagcaatt
    27961 cgccagttga aaaagtccgt gaagacacca ctaattaagc gtccgctcat gccgccaatt
    28021 gagttgccgc tgatatacaa ccccatcgaa aaggccacga aactggggtg gatttcctcg
    28081 ctaagataag tcatgccaac agctgccacg ccacttaacg aaagcccaat caaggcgcgc
    28141 ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca aatggaggcc
    28201 aacagtagcg ccgtgaccat cactggtttg cgaccaatgg catcggatag cgggccagta
    28261 aatagcaaac cgatagccaa catcgccgtg gaaatggaca gtgaaatact actgttcgcg
    28321 ggcgttaagc caaactcctg cgaaagcacc ggaaggatag gctgcacaca atagagaagt
    28381 gcaaaggttg ccagtccggc agagaacagc gccagggtga cgcgcataaa ttgcggcgta
    28441 ccgcgtttaa taaattgatt tggctgagaa atgctttgct tgtcagtgtc gcttgccgga
    28501 gcgccatcaa cagttgtagt acggctcact tgaaatcctt gctaaatatg cctgtggatc
    28561 aggcttatac atagggtagg aaaatcgaat tgttctgtct aatatattaa taatctcaaa
    28621 taagatgttt taaatatgaa tattgaactt cgtcatctgc gttactttgt tgctgttgcg
    28681 gaagagctgc attttgggcg cgcggctgcc cgcctgaata tttcgcaacc gccgctaagt
    28741 cagcagattc aggcactgga gcaacaaatc ggtgcccgac tgctggcacg aactaatcgc
    28801 agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca aatcctgtct
    28861 atggtggatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc gggggagttg
    28921 cgtattggtt ttacttcgtc cgctcctttt attcgggcgg tgtccgatac gttatcgctg
    28981 tttcgccgtg attatcctga tgtccattta caaacccgcg aaatgaacac tcgcgagcaa
    29041 atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac agcgttaccg
    29101 gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat cccgtacgat
    29161 catcccctgg caaataatcc gagtgtaaca ctggctgaac tggctgaact ggctgaactg
    29221 gcgaaagaac cctttgtctt ttttgatccg cacgtcggga ctgggctgta tgacgatatt
    29281 ctcggactga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt gggcgaggca
    29341 atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc tgcgtcattt
    29401 aaacgtgttc agctcaacga aatgcgctgg gtgccgattg ctgaagagga tgcggtttct
    29461 gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagctgcgcg taactttcgt
    29521 attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc gaaaaatgtg
    29581 ctgttaatca catgcctaag taaaaatttg acgacacgta ttgaagtgct tcaccatagc
    29641 ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct gaaaaccagc
    29701 ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc ctgattgatc
    29761 agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct cctgccagta
    29821 tcactaaaat tgtccgtgag atgctcgaag cacacctggt gcgagagctg gaaatcaaag
    29881 aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt tgaaactgaa gcctggcact
    29941 atctttctct gcgcattagt cgcggggaga ttttccttgc tctgcgcgat ctgagcagca
    30001 aactggtggt ggaagagtcg caggaactgg cgttaaaaga tgacttgcca ttgctggatc
    30061 gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt gagcgtctaa
    30121 cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt gtacatcgca
    30181 tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag cagcataccg
    30241 gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag gccttgtttg
    30301 gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac gtgggggtgg
    30361 gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg gaaataggcc
    30421 acacacaggt cgacccatat ggtaaacgct gttattgcgg gaaccacggc tgcctcgaaa
    30481 ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat caatccatga
    30541 gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg gcattgcgcg
    30601 gcgatctact ggcaaaagac atcattatcg gggtgggcgc gcatgtaggg cgcattcttg
    30661 ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg ttaagtaaag
    30721 cggcagatat cctcttcccg gtcatctcag acagcatccg tcagcaggcc cttcctgcgt
    30781 atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg atggcaggcg
    30841 ctgcactggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg ttgcagggtt
    30901 aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca ggaaagcata
    30961 acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat aacggtggag
    31021 ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg gaaaacggtg
    31081 gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaagggttgc gggatataaa
    31141 cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga tgccttggtg
    31201 ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat cgcgttaagc
    31261 gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat ttcaaacggc
    31321 ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg cggctggcgc
    31381 agtctgatga atgatttgcg tccactctct gaatgggtag tgcaggaaca actgccggtg
    31441 ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac agctcaggcg
    31501 atcgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa cccaggactg
    31561 gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc gctcattggt
    31621 gaactgcctt atctgccgcg cgccgaacag cgtgaactgg ggcaatacat ccgcttagct
    31681 atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg aaattaccga
    31741 cgcaattacg caggcaatca ataaaccggg gagtagctga tactccccgg tcatttcaca
    31801 tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg tcgccatccc
    31861 ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc ccaggctacg
    31921 accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga caccaccggg
    31981 tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga tcccggcaat
    32041 gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc cgttgcccca
    32101 cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg ccagttgcca
    32161 gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta acgttaacaa
    32221 cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat agtcacgagc
    32281 ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat taatcagatt
    32341 gctaaccagc catgccacga tggcggaaat aatcaccggg ccgagagagg ccaacatcat
    32401 agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac gataggccgc
    32461 agccattccc gccgcggccc cacaggcgat ccataatttc cattcctggc gtggcgtaaa
    32521 acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac cttcgcgacc
    32581 aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca ggcttgctgc
    32641 gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg catgagggcg
    32701 ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc cgccgagcgc
    32761 cggagttagc agccgtcgcc aggaggaaag gtttgtcgct gcattgacca gactgccgga
    32821 gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc cggcaacggc
    32881 aaagacagcg agaataccga cgactgttgc gataagcaga cggcggaaca tagtgcgcag
    32941 atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca cgggcgacta
    33001 ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc tttaagagat aaaaaaacgt
    33061 gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc accatccggc aatattacgg
    33121 tgatcctatc gaaagagtgg tttaaccgcg acaggaatca acagttgtga ttgccattgc
    33181 gccagcgtta agcgagccag ttcacccagt gcacggtaga aagggtgttc tgctttttcg
    33241 ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt gccatgccag caattcttcg
    33301 cattctgtct ggcgaccatt ctccgccaac cacgcagcca tcaacagcag tgaaccaaaa
    33361 tgatcttccg gttcgttttg cttcatttca aactgaatgc ctttctcgcg catccactga
    33421 cgaagtgcca atgttgaatc gccaaacagc acagattcgc gatccagcca gaccgacccc
    33481 cacggcggag acggcagtgc ccacgggccg acaaacaaac gctgccaggc ctgggcgtga
    33541 gtctcttcac actgtgtctg aaaagccgtc accagcggcg ctaatgacgc ttctggtaga
    33601 ggccactgag tttcccagcc gtcactggtg agtaccgcaa ccagaggtgc ggcttccgca
    33661 ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg ccgcgacaga aaaattatct
    33721 tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg cgggttgccc cgcaccaatt
    33781 tgttaacctg caatagccat tcctactgtc atgtgcaggc cataaaagag cacacgcccg
    33841 atcatctcac caacaagtat gaggattagc cccagaacaa gtccggcaac gtgaggttca
    33901 cggcgacgaa tgagcggaca aagccagcat ccaagacctg cgcataacaa taccacacgc
    33961 cagacctgta aggaggcgta atctggtacc agtgcgctgg cttgttgcac ggatgagtga
    34021 atagacgcca gagacaaacc ttgcagcaca atcacctcgg cacaagcgat cagcgccaga
    34081 acgctaatga tggcaaatgg tgtggtatta aatgtaacgc gggctgcccg cagaattgcg
    34141 gcagcaagta ttggaccgct caacaatacc gtcaggaaga aagccagcgt agtgtaaccg
    34201 ttatgccagg ttggcacggt gtcgatttga tacacgcagg tcatcatcca gacgaaaatg
    34261 acgccgagcg ccatgctgac aagtaaccag agtttcccca acgcttgcgg cattttaccg
    34321 atgaccgcca ccagccacca tagtccgcca acggcaaaaa atatggaacc ggcagcaatc
    34381 tcgttactca gaccagaagc tccgattcga ttcagcgagt taaacgctcg cagtggcgat
    34441 cccaggtgca tgacagaggc aataaacccg atgcccatca acagccagag gaaaaacatg
    34501 ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg cgaaccagcc aattccgcta
    34561 acaatcagcg cgcctactac gcattgaccc aatacggtaa agataaccag tggccattca
    34621 tgccatccat ttcccatttt acacttcctc cggattagcc agataaccag tggtatcgcc
    34681 ggtcgggcgg ctgttggcgt taggtttgat aacgatattg ggttttgtga aatgcgcgcg
    34741 cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg cgcagctctt caattggacc
    34801 gaactcaagt gcacgcagcg ggcaggattc gacgcatatg ggttgtttcc cctcggcgac
    34861 gcgcgaataa caaccatcgc acttcgtcat gtgccctttt tcagcattgt actgtggtgc
    34921 gccgtacgga caagccatgt ggcagtagcg gcagccgata caaacatctt catcaaccac
    34981 cacaaagcca tcttcacgct tatgcattgc gccgctgggg cagacttttg tacatgcggg
    35041 gtcatcgcaa tggttacagg agatggagag ataataagcg aacacgttct ggtgccagat
    35101 gccattatct tcctgccagt cgccgccagc atattcataa atacggcgga aactgacttc
    35161 cgggccaaga tctttaaaat ctttgcacgc cagttcgcag gttttacagc cagtacagcg
    35221 gctggaatcg ataaaaaatc catattgtgt ggtcatgggc tactccttaa accttttcga
    35281 tctggacaag attgctgtgc gacgggtttc cctttgccag cggtgaaggg cggtgagagg
    35341 tcagaatatt gatactgccg ccgtgatcga cccggtcacc aaacatatcc gctttaagcc
    35401 acgcaccttg cccgatggcg gtaacgccag gcagaatacg cggagtcact tttgcggcaa
    35461 tcagcatttc tccattattg ttaaataccc gcacggtatc gccatgacgg ataccgcgtg
    35521 cctgagcatc aatggggttg atccacacct cttgtgggca ggcctgctgt aacacatcaa
    35581 tattgccgta gctggagtgg gtacgcgctt tggtttggcg acttcactca gcatccagta
    35641 gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca catagcccat
    35701 attccctgca gattcatgag agataaggtc ttcttgctct gttggcatca ggtcaggcaa
    35761 caggatatcg caatacttag ccgaggccgt catgaagtgg tcaatgccaa caatcatctc
    35821 gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat gttgattgat
    35881 caatgtgtta ctggcgtagc accataaaaa cttgatgggg acatccagtt tttcttttcc
    35941 acgaacacca tcacgggtcg cggtcatttt cgtaccatgg tcgatggcat ctgtccatgt
    36001 aaaaacggaa atctgcgttt taacaggatt ctcgagcatc gggaaccatt ctacccccag
    36061 atcccagcta ccttcgcgta cacctgagtt gccgccgttt atgccgacgt taccggtaag
    36121 aacggaaagc atggcaatag cgcgggacgt ttgttcgccg ttggagtgtc gttgtggccc
    36181 ccaaccctga caaatataag caggttttgc tgaaccgatc tctcgtgcca actggataat
    36241 tttttctgcc gggatgctgg tgatttttgc tgcccattcc ggcgttttag ctatgccgtc
    36301 aggcccttcg cccagaatat aggctttata atgcgcgtta cgtggtgcgt tggcgggcag
    36361 cgttttttca tcgtaaccaa cacaatattt gtcgagaaat ggctgatcga ccatgttttc
    36421 agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca tcggtgccag ggcgaatggg
    36481 cagccattca tcttcacgcc cggcagctgt gtcgttatag cgtggatcga tgacgatcat
    36541 gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa gtgacaccac cgccgctcat
    36601 ccgcgtttct gccgggttat ttccgaacat aacgaccagt ttcgtattgg cgatatcatc
    36661 cgggctgttg ccatcattgg caccgaacat ataactcatt gcggcactga tctgtgcggt
    36721 actgtagctg ccatagcgac tgagaaaacc cccgcaagag ttcatcagac ggtacgggac
    36781 gttcgagttg gtgatatttc cgccatctac gcctgttccg tacaggacat gtacagcctc
    36841 attgccgtaa tctttcagga tccgccgaag attatcactg atggtatcca gggcttcgtc
    36901 ccaacttatc cgttcaaatt taccttcacc gcgctcgccg acgcgcttca tgggatattt
    36961 caacctgtca ggatgattca tccgtcggcg gatagagcgc ccgcgtaaac atgcccgaac
    37021 ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac tccacccagt acacggtgtc
    37081 atctttcaca tgcaaacgta gcagacagcg gctcccgcag ttaacggtgc aggaactcca
    37141 gaccgttttc tcttctaccg gagcctctgc cgcccggacc atttgggaaa atggcagagt
    37201 gaaagcactg cttgccagcg cgagactgcc aagtgcggag gttttcatca gacttctatg
    37261 gctgatttca gccttcatga gcgcctctgt ggtatggatt ttcatcatta ctcacttatt
    37321 gcttttcaaa caaaatgtca tgccagaatt tatggttgta gtgggttata ttttttcgat
    37381 ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt ggtgaagggc gcagagtggt
    37441 tagcgtattc acacatccgc catggtcgat tttatcgcca gacatattgg gctcgtgcca
    37501 ggctccctgg cccatagcgc taactccagg gagaatgcgt ggtgttactt tggctggtag
    37561 ccgaacttcg ccacgatggt taaacacccg caccatatcg ccgttggcaa tcccacgttt
    37621 ctgtgcatct atagggttga tccacacctc ctgacggcag gctgccttca ggacatcaat
    37681 gttgccgtag gtcgagtgag tacgggattt gtaatggaaa ccaaacagtt gcagtgggaa
    37741 ggttctacgt tcaggggagt cccagccttc aaaggttgag gcataaactg gcagtgggct
    37801 tatcacttca tctttttcca gttcccaggt acgggcaatt tccgccagct tgctggaata
    37861 aatttcaatc ttaccggaag gcgttttaag tggatttgcc tcggggtcgt cacgaaatgc
    37921 tttgtaggcg acaaaatggc cattgggatc tttacgctta tagataccca tttttttcag
    37981 ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt ttggcgtaca gatgttgtaa
    38041 ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag acgtcaggtc caagacgttt
    38101 cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg aatttttcgc tggtgactgg
    38161 ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg ttaggaataa tatcttcctg
    38221 ctcaacggtc atcaggtctt ccagcagaat gtcggcatat tttgccgatg aggtcataaa
    38281 gttttcgatg accacaatca tttcgcattt cgattcgtcc tgcagaattt catgcgtttt
    38341 gttgatgtca gaatgctgat taacgagggt atttcccgcg tagttccaga tggactccat
    38401 tgccagacgg atggatggac ttgaagtagt tgactcaatg ctacttaagg tcctgatatg
    38461 actgacatta atcatgataa cactgtatat ccatccagtt ctttctctgg gtctccctat
    38521 cggtatcagc aagtctacac ctcaagccct gacacaaggc tgacacaaat caatctactc
    38581 agcgtagcta agcctcagtt agtccgcaga gccaatggtc gatatactat ccgcttcaga
    38641 ctcaagggac agaccacacc gtttctttca gtatcgaccc gcagcacaga caggagagta
    38701 gccacaatgc gtcagagaga gcttgcagcc accgctaagg ccttcatgtt ggataggcct
    38761 gaagtctcac tacaggagct tacagagcat ctccggtcta tggctgagca gttcctgaca
    38821 gacgctagtg acgattactg gaatggact
//
LOCUS       DAJTYL010000026        36721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYL010000026 DAJTYL010000000
VERSION     DAJTYL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.026"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..874)
                     /gene="yhdJ"
                     /locus_tag="OO572_001299"
     CDS             complement(<1..874)
                     /gene="yhdJ"
                     /locus_tag="OO572_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6660363.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDL"
     gene            complement(960..1256)
                     /gene="fis"
                     /locus_tag="OO572_001300"
     CDS             complement(960..1256)
                     /gene="fis"
                     /locus_tag="OO572_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6660364.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1282..2247)
                     /gene="dusB"
                     /locus_tag="OO572_001301"
     CDS             complement(1282..2247)
                     /gene="dusB"
                     /locus_tag="OO572_001301"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6660365.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2576..3457)
                     /gene="prmA"
                     /locus_tag="OO572_001302"
     CDS             complement(2576..3457)
                     /gene="prmA"
                     /locus_tag="OO572_001302"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6660366.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3469..4920)
                     /gene="panF"
                     /locus_tag="OO572_001303"
     CDS             complement(3469..4920)
                     /gene="panF"
                     /locus_tag="OO572_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6660367.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4910..5152)
                     /locus_tag="OO572_001304"
     CDS             complement(4910..5152)
                     /locus_tag="OO572_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6660368.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5261..6610)
                     /gene="accC"
                     /locus_tag="OO572_001305"
     CDS             complement(5261..6610)
                     /gene="accC"
                     /locus_tag="OO572_001305"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6660369.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6621..7091)
                     /gene="accB"
                     /locus_tag="OO572_001306"
                     /gene_synonym="fabE"
     CDS             complement(6621..7091)
                     /gene="accB"
                     /locus_tag="OO572_001306"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6660370.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8069..9043)
                     /locus_tag="OO572_001307"
     CDS             complement(8069..9043)
                     /locus_tag="OO572_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6660371.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9195..11135
                     /gene="csrD"
                     /locus_tag="OO572_001308"
     CDS             9195..11135
                     /gene="csrD"
                     /locus_tag="OO572_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6660372.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11440..12483
                     /gene="mreB"
                     /locus_tag="OO572_001309"
     CDS             11440..12483
                     /gene="mreB"
                     /locus_tag="OO572_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6660373.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12549..13652
                     /gene="mreC"
                     /locus_tag="OO572_001310"
     CDS             12549..13652
                     /gene="mreC"
                     /locus_tag="OO572_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6660374.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13652..14140
                     /gene="mreD"
                     /locus_tag="OO572_001311"
     CDS             13652..14140
                     /gene="mreD"
                     /locus_tag="OO572_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6660375.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14149..14742
                     /gene="yhdE"
                     /locus_tag="OO572_001312"
     CDS             14149..14742
                     /gene="yhdE"
                     /locus_tag="OO572_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6660376.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14732..16201
                     /gene="rng"
                     /locus_tag="OO572_001313"
     CDS             14732..16201
                     /gene="rng"
                     /locus_tag="OO572_001313"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6660377.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16269..20069
                     /gene="yhdP"
                     /locus_tag="OO572_001314"
     CDS             16269..20069
                     /gene="yhdP"
                     /locus_tag="OO572_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6660378.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20225..21670
                     /gene="tldD"
                     /locus_tag="OO572_001315"
     CDS             20225..21670
                     /gene="tldD"
                     /locus_tag="OO572_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6660379.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21798..22727)
                     /gene="aaeR"
                     /locus_tag="OO572_001316"
     CDS             complement(21798..22727)
                     /gene="aaeR"
                     /locus_tag="OO572_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6660380.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22910..23113
                     /locus_tag="OO572_001317"
     CDS             22910..23113
                     /locus_tag="OO572_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6660381.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23121..24053
                     /gene="aaeA"
                     /locus_tag="OO572_001318"
     CDS             23121..24053
                     /gene="aaeA"
                     /locus_tag="OO572_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6660382.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24059..26026
                     /gene="aaeB"
                     /locus_tag="OO572_001319"
     CDS             24059..26026
                     /gene="aaeB"
                     /locus_tag="OO572_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6660383.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26118..26390
                     /locus_tag="OO572_001320"
     CDS             26118..26390
                     /locus_tag="OO572_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6660384.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26446..26709)
                     /gene="yhcN"
                     /locus_tag="OO572_001321"
     CDS             complement(26446..26709)
                     /gene="yhcN"
                     /locus_tag="OO572_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6660385.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27074..27544)
                     /gene="argR"
                     /locus_tag="OO572_001322"
     CDS             complement(27074..27544)
                     /gene="argR"
                     /locus_tag="OO572_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6660386.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27979..28917
                     /gene="mdh"
                     /locus_tag="OO572_001323"
     CDS             27979..28917
                     /gene="mdh"
                     /locus_tag="OO572_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6660387.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28979..30046)
                     /gene="degS"
                     /locus_tag="OO572_001324"
     CDS             complement(28979..30046)
                     /gene="degS"
                     /locus_tag="OO572_001324"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6660388.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30136..31483)
                     /gene="degQ"
                     /locus_tag="OO572_001325"
                     /pseudo
     CDS             complement(30136..31483)
                     /gene="degQ"
                     /locus_tag="OO572_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31637..32035)
                     /locus_tag="OO572_001326"
     CDS             complement(31637..32035)
                     /locus_tag="OO572_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6660389.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32229..33356
                     /gene="zapE"
                     /locus_tag="OO572_001327"
     CDS             32229..33356
                     /gene="zapE"
                     /locus_tag="OO572_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6660390.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33575..34003
                     /gene="rplM"
                     /locus_tag="OO572_001328"
     CDS             33575..34003
                     /gene="rplM"
                     /locus_tag="OO572_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6660391.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34019..34411
                     /gene="rpsI"
                     /locus_tag="OO572_001329"
     CDS             34019..34411
                     /gene="rpsI"
                     /locus_tag="OO572_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6660392.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34806..35444
                     /gene="sspA"
                     /locus_tag="OO572_001330"
     CDS             34806..35444
                     /gene="sspA"
                     /locus_tag="OO572_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6660393.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35450..35947
                     /gene="sspB"
                     /locus_tag="OO572_001331"
     CDS             35450..35947
                     /gene="sspB"
                     /locus_tag="OO572_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6660394.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35990..>36721)
                     /gene="dcuC"
                     /locus_tag="OO572_001332"
     CDS             complement(35990..>36721)
                     /gene="dcuC"
                     /locus_tag="OO572_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6660395.1"
                     /translation="FFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILML
                     GSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFV
                     NVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGN
                     APFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFA
                     VVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8851 a         9185 c         9691 g         8994 t
ORIGIN      
        1 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
       61 ttttcacttt cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc
      121 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg
      181 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
      241 aagaggcgag aataatgcgt ttcagtaagg ctttcggttt ttgcgtcggg tggttctcat
      301 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
      361 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
      421 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
      481 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
      541 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
      601 gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc
      661 actctgcaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
      721 gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagagcaga tcgacgcttt
      781 cagtaggtag ctttttaagt tcggcaaagg catcaccgtg aataatggtc ttagcttcat
      841 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
      901 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
      961 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     1021 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     1081 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     1141 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     1201 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     1261 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     1321 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     1381 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     1441 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     1501 tgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     1561 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     1621 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     1681 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     1741 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     1801 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     1861 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     1921 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     1981 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggagcgctac cagcaatttg
     2041 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     2101 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     2161 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     2221 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     2281 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     2341 gatggatcaa agaattgcat ttaaaatgag cgtggtgcga taattactca taaaaatcat
     2401 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     2461 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataatcggca aatttacgtg
     2521 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggtcaat
     2581 tcttacgacc ggtaatacgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     2641 tatcggcata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     2701 gcaaaccgcc tgaaactggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     2761 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     2821 agagttccag acggtcagaa acgccattac gttcggcgtt atcgcggctg gcctgaatcg
     2881 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg
     2941 ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt accggttaaa tcgaggctgt
     3001 cgagccattg caggcacaga gaggtggttg gatgggtacc cgtaccaaac gccagccctg
     3061 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     3121 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     3181 tatcttctag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     3241 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     3301 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     3361 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tccgcgttcg
     3421 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     3481 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     3541 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     3601 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     3661 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     3721 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     3781 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     3841 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgcag
     3901 cagttgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     3961 agcaaaaggt ggcagcactt ttaccattaa cgtcgggatc accagatctg gtacggtgag
     4021 atcggggatc actgcacgac ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc
     4081 gactataatc gtaccgatga tgatcccccg gtgtactgct ttgctgtctt tataagagat
     4141 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
     4201 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     4261 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     4321 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     4381 ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa tcagcagccc
     4441 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     4501 ggtcatcgca ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     4561 actctggtag cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag
     4621 aatcgcaaac ttcttgccga gaataccgag tgaaagccag acagcaggaa gctgaatcat
     4681 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
     4741 actggcactg atataggtcg cggtgagagt catcgccagc acaataccgc ccatagagcg
     4801 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     4861 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
     4921 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
     4981 taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
     5041 cgggggcaac gccaggtaag taagcgtcta ctaaccaaac tgccagatac aaaagggtca
     5101 gccccagcgc ccagcgggcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
     5161 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
     5221 aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga
     5281 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     5341 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     5401 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     5461 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
     5521 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
     5581 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     5641 tgatcgacag cggttgaccg gcagcgatac gcagctgttc tttgatcagg tcaacgccgg
     5701 tgatcatttc tgtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     5761 actcgccgtt ttcgaacagg aactcgaaag tacctgcacc gcgatagccg atatcaacac
     5821 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     5881 gtgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     5941 agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattctcca
     6001 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     6061 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccgccgc
     6121 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcgcggtttt
     6181 tatccatatc gtcgcccagc gggccgtcag aaccaggtac gcaaggaacg cccgcttttt
     6241 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     6301 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     6361 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
     6421 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     6481 gtaatacgtg ttttagatcg cgatccgtgc tggagtgcac agcgacagtc ttgatgccca
     6541 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     6601 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     6661 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     6721 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     6781 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
     6841 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     6901 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
     6961 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
     7021 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     7081 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     7141 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
     7201 gggacgccgg tctattttgc cccagagtgt caatgttaga cttgacggac attgtgcagc
     7261 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     7321 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     7381 caattggcag ctaaatactg gtcttatcag ggaatataat caacagctaa catgtaaata
     7441 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     7501 gacggaactt cttataacgt aaggtgaaaa gctgaaaagc cagccctgcg aagatgaggg
     7561 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     7621 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
     7681 tagatgtgaa tgctaaagca cgcaaaatca accaactgac aattctcagc attaaatagg
     7741 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat
     7801 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
     7861 aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
     7921 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
     7981 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
     8041 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
     8101 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
     8161 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
     8221 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
     8281 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
     8341 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
     8401 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
     8461 taccgctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
     8521 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
     8581 cacccgtcac gacaatctca ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
     8641 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
     8701 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
     8761 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
     8821 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
     8881 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
     8941 gaacatcgac cgtgacatcg ccctccggca ggcgacgttc gtccagagtc tgtactgatg
     9001 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
     9061 atgatggatt ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat
     9121 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
     9181 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
     9241 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gccattcagt
     9301 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
     9361 atgacttcag cgcattaagg ccacaaatta ccgaattaat ggtgtcagca gatatcgttc
     9421 gtgtagacct gctgcatggt gataaacagg tttataccct ggccagaaat ggtagttatc
     9481 gtccggttgg caccagcgat ctgtttcgtg aactgagcgt tccgttgata aagcatccgg
     9541 ggatgtcgtt gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tcgttgatga
     9601 ccaccgcgcc gctcacgggg gcgattggct ttatcattct tatgctcttt ctggcggtac
     9661 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
     9721 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
     9781 gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
     9841 ttgatacgct gatccgctct tatgccgcac aggacgtgaa aaccggcctc aataaccgac
     9901 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
     9961 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatacc tgggggcaca
    10021 gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct
    10081 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    10141 ggacgttaaa agaggcagag agcatagcca gtcagttaat caaagccgtc gataccttgc
    10201 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgt gcctggcgta
    10261 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    10321 tgcagggtgg caatagctgg gctatttatg atgactcgtt gcctgaaaaa ggacgcggta
    10381 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    10441 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    10501 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    10561 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    10621 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgtccg cgttttcagc
    10681 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    10741 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    10801 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    10861 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcaggaaca
    10921 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    10981 ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc
    11041 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    11101 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    11161 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    11221 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    11281 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    11341 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    11401 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    11461 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    11521 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    11581 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    11641 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    11701 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    11761 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    11821 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    11881 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    11941 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    12001 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    12061 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    12121 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    12181 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    12241 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    12301 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    12361 atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc
    12421 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    12481 taatcggatg caggcagggg aagcgtctgt ttaccctgcc tggtctgata cgagaatacg
    12541 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    12601 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    12661 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    12721 aattgctgga tggcatatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    12781 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    12841 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    12901 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    12961 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    13021 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    13081 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    13141 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    13201 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    13261 tctcttccgt aaaactcgat acccagcgcg cttatactgt gatccaggcg cgtccgactg
    13321 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    13381 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    13441 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    13501 tcgctcagcc gactccgcag caaccggcga caggaaatgc agctactgcg cctgttgcgc
    13561 cgacacagcc cgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    13621 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgctg
    13681 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    13741 cctgattgtt ttccggccaa actgggtgtt acttatcttg ttgtattgga tcctggcctt
    13801 gccccatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    13861 cggctcgatg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    13921 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    13981 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    14041 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    14101 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    14161 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt
    14221 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    14281 gttgtgcgtc tggcacgcga gaaagcacag gcgggtgtcg cgcaaacggc acaggatctc
    14341 ccggtgctgg gtgcggatat tatcgttatc ctgaacggag aagtgctcga gaaaccgcgc
    14401 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    14461 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    14521 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    14581 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    14641 ggcagctatc acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat
    14701 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    14761 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    14821 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    14881 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    14941 atccgacatc atgccgcata ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    15001 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    15061 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    15121 ggtatttatg ccaggggctt ctcacgttgg ggtttcccaa cgaattgaaa gcgaatcaga
    15181 acgtgaacgt ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    15241 ccgcaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    15301 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    15361 cggcgaactg gcgctggcgc agcgtgttct gcgtgatttt gccgacgccg aactggaccg
    15421 cattcgcgtt gactcacgcc tgacttacga agcattgctg gagtttacct cggagtacat
    15481 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    15541 tgatgtcgaa aacgaaatcc agcgagcgtt ggaacgcaaa gtagaactga aatccggtgg
    15601 ttatctgatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    15661 tgtcggtcat cgcaatctgg acgacactat tttcaatacc aatattgaag cgacgcaggc
    15721 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    15781 tatgaataat gaagatcacc gccgccgcgt gctgcattcg ctggagcagg cgttgagcaa
    15841 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    15901 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    15961 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    16021 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    16081 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    16141 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    16201 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    16261 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    16321 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttaac gcctggcgtc
    16381 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    16441 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    16501 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    16561 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    16621 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    16681 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt tttctgacgc
    16741 catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    16801 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    16861 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    16921 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    16981 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    17041 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    17101 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    17161 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    17221 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    17281 ggattcgcgc tagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    17341 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    17401 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    17461 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    17521 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    17581 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    17641 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    17701 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    17761 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact
    17821 tgatgggtaa agacctggtt gattatttaa gtggtgcgat tcagggcggt gaagcggata
    17881 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    17941 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    18001 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    18061 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    18121 caaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    18181 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    18241 acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgaa ctggtaaccg
    18301 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcatcc
    18361 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    18421 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    18481 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    18541 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    18601 atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg
    18661 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    18721 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    18781 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    18841 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactt aatatgccgc
    18901 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    18961 gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    19021 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    19081 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    19141 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    19201 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    19261 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    19321 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    19381 ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac
    19441 tgcgtgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    19501 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg
    19561 aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    19621 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    19681 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    19741 ccgcgtggat taaagacggc gttatgcaca ccgacgacac gctggtggat ggcctggagg
    19801 cggatatcgc catgaaaggg tcggtaaatc tggtacgccg cgacctgaat atggaagcgg
    19861 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    19921 ttgttggcgc ggcagtgttt gccgccagta aagtgctggg gccgctgtgg agcaaagtct
    19981 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    20041 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    20101 atatgataat cgttgccaaa ggccaacgag ccggaacata actgtaggcc ggatagggcg
    20161 ttcacgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    20221 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca
    20281 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    20341 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    20401 ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    20461 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    20521 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    20581 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    20641 tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    20701 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    20761 ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    20821 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    20881 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    20941 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    21001 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gtggcacttc
    21061 agtatttagt ggacaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    21121 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gatgaaggta cgccaggcca
    21181 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    21241 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    21301 catgccgcgt atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aagaaattat
    21361 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    21421 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    21481 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    21541 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    21601 aagtttgccg gttggcgtgg gccagccaac gttgaaagtc gataacctga ctgttggcgg
    21661 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    21721 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    21781 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    21841 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    21901 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    21961 aggatctcca gctccccacg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    22021 atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt cacaaatctc
    22081 ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg
    22141 ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt ttccggtatg
    22201 ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc gccaagacgg
    22261 cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg
    22321 gcaatcaggt cgggggctgg aattccggta accagattgg cgctcaaacc tgggtattct
    22381 ttcagcattt tggctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca
    22441 atgcgtagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc
    22501 acttcatgaa gcatacgacg gcagccctgg tagtaaattc taccggcttc ggtcaggcca
    22561 atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc
    22621 gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca
    22681 aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac
    22741 ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc taatatatta
    22801 aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac
    22861 agatgctgaa aataataacg cctgctctct ctatacaacc aaggtcaaca tgagtctgtt
    22921 tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat tgcttttatc
    22981 actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt
    23041 ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg
    23101 actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca
    23161 cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc tattacaccg
    23221 aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg
    23281 tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga
    23341 tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg
    23401 ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc
    23461 tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg
    23521 ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac
    23581 gcacggtgat ccgagcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg
    23641 agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac
    23701 tggcctatat ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt gcagagatca
    23761 cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gctggggtca
    23821 ccaacgccag cagcacgcgt gacgacaaag ggatggcgac catcgactct gaccttgaat
    23881 gggtgcgttt ggcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca
    23941 tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa
    24001 gccaggattc gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat
    24061 gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat
    24121 tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact
    24181 gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg
    24241 cgctattcgc tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg
    24301 actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat
    24361 ctgggccggt ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg
    24421 ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct
    24481 tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat
    24541 tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc gagagctgga
    24601 aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga
    24661 agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcactac aaggcatgcg
    24721 cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat
    24781 caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac
    24841 gcgcccggaa ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc
    24901 gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ctggggaacg
    24961 ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa
    25021 gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga
    25081 accggaagta aaagtagagt cagccgaacg tcatcatgca atggttaact tctggcgaac
    25141 cacactttcc tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag
    25201 tggtgcaatg gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg
    25261 catggtggcg atcgacttta tctatgggac gctggccgcg ctgccgttag ggctgctcta
    25321 ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc
    25381 agtgctggga ttcttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc
    25441 actggccagc accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca
    25501 gtttctcgac agcgcattag gacaaatcgt cggctgtgtg ctcgcgttca ccgttatttt
    25561 gctggtgcgg gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttc
    25621 tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc
    25681 actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg
    25741 cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa
    25801 cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc
    25861 ccgtagcgat gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta
    25921 ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct
    25981 ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc
    26041 caaaagcgtc ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa
    26101 cgtgacggtg gcaacagatg aatatttatg cctttgattt tgatgagatt gagagtcagg
    26161 aggattttta tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc
    26221 tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat
    26281 ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg
    26341 aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa
    26401 agcccacgaa ccgggggcaa tatcgtcgga caaaacgatg agggtttatt tgtacagttc
    26461 agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc
    26521 tttctcttcc gcttttttgt tcagcatttc acgtatatcc attggcgaag acgccacacc
    26581 acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagcat caatggagtc
    26641 ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt
    26701 gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat
    26761 atcacaaaat caagtataca ctaattattg aactaattaa taccacacta actgtttcag
    26821 ttgatactaa gtgttaaaaa tgtgaatttt aataacaaaa aagtataaaa aacacggtta
    26881 tttattatta attatagtat tttcaattag ataatgaatt ttgcataatg tgcttttttt
    26941 accgtatgta ttcattttgt gaatgatgtg tcgcggtaaa acgcactatt cgttaaatgt
    27001 atcgcaggag aaagaagggg aatgagaggg ataagcaaca ttttccccgc cgccagaagc
    27061 gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt
    27121 tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca
    27181 gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag
    27241 ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca
    27301 atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt
    27361 tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat
    27421 taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt
    27481 tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc
    27541 gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta
    27601 tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca
    27661 aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat
    27721 taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt
    27781 tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg
    27841 gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg
    27901 taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa
    27961 tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag
    28021 gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat
    28081 atcgctccag tgactcccgg tgtggccgtc gatctgagcc atatccctac tgctgtgaaa
    28141 atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt
    28201 atctctgcag gcgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac
    28261 gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa actgcccgaa agcgtgcatt
    28321 ggtattatca ctaacccggt taacaccaca gttgcaattg ctgctgaagt gctgaaaaaa
    28381 gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc
    28441 aacacctttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt
    28501 ggcggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt
    28561 accgagcagg aagtggctga tctgaccaaa cgtatccaga acgcgggtac tgaggtggtt
    28621 gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt
    28681 ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt
    28741 gaaggcgacg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc
    28801 gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagaacgc gctggaaggt
    28861 atgctggata cgctcaagaa agatatcgcc ctgggcaaag agttcgttaa taagtaattg
    28921 attagcgaat aataaaaaac cggagcacag actccggttt ttgttttgag cactcgactt
    28981 aattggttgc cggatattcc tgaatggtga cctgcagcgt taactgctta tcatcacgca
    29041 tcactacaac cgggatcacc gaaccagggc gaatttccgc cacctgatcc atcgtctcca
    29101 gagcagagat ggccggtttg ttatccaccg aaataatcag atcgttgacc tgaatacccg
    29161 cattcgccgc tgggccgtca ggtgacactt cattaaccac gatcccttgc agttgatcta
    29221 taccaccgct ctgcgcgtgc agtggtgcga tctcgcgtcc gccgatacca atgtagccgc
    29281 ggatcacgcg accatcgcgg atcagcttat ccataatttt ggttgctaac tggaaaggaa
    29341 tcgcaaagcc gataccttcc ggcgtttcgc catcgttact cttatcaaac gacagcgtgt
    29401 taatgcccat cagttctccc agcgagttca ccagcgcgcc gccagagtta ccgtggttaa
    29461 tggaagcatc ggtttgtagg aagttttgcc gcccggtcgg gttcagaccg attcgacccg
    29521 tggcactaat aatcccttgg gtaatggtct gcccgaggtt gtacgggtta ccgatcgcca
    29581 gtactacgtc gccaatgtgc ggtacgcgac gtgcattaat tggaatggta ggtaaaccgc
    29641 cagtggcatt aattttaagt accgccagat cggttagaga gtcagatccc accagcaatg
    29701 cttcaaatac acgtccatcc tgtaaggcga cgatgatctg atcggcgtcg ttgatgacgt
    29761 gtttattggt gatgatataa ccgcgttgat ccatgattac accggatccc agggtgcgga
    29821 tctcaagctg gttgtgagag ttggtgttca aaccacggtt gtaaacgtta accaccgctg
    29881 gcgcggcgcg gcgaaccgcc agattatagc tggcaggcgt ctcatcggta ctgtcaaatt
    29941 gcggagtgga aagcgggtta aggctgcgca gcgaaggcat ggcaaccagc agaatagcgc
    30001 cgacaattaa tccaatcgca acggaacgta agagcttcac aaacatgatg gaggcgtcat
    30061 taaaaaaggg aacggcagca gcataccacg agttaaccgg acatcacacg tcagcctgat
    30121 gcccggttta cgacattaac gcatcagcag atagatgctt tcattgccgc gtacaatttg
    30181 cagggcgatg atggccggtt ttgccgccag cactttacgc atttcagcaa tcgagttcac
    30241 ccgatcgcgg ttgacgccaa tgatcacatc gtctttttgc aagccagcct gagcagctgg
    30301 gcttcctttg acaacttcat ctatcttaat acctttgccg ccatctttta gctgaccatc
    30361 gctcaacgtt gcaccttcca gcgctggccg acgaagaggt gctggtatca agcgtcactt
    30421 ctacttccag tggtttgccg ttacgcagca ggccaagctt cactttcgtg cccggctctg
    30481 tagtcgcgat acgagagcgc aactcagcaa agctattcag cggtttgccg ttgaggctgg
    30541 taataatatc gcccgctttg acgcccgctt tcgctgagcc agaacttggc aacacttcgc
    30601 tgacaaacgc gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg gcactcatct
    30661 cggtgccttt gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata agctgctgcg
    30721 ccagtgttcg cgccatatta ctggggatgg caaatccaat cccgacgctc ccgccgccag
    30781 gcgcaaggat tgcagtgttg atgccaatta actcaccgtt aaggtttaat agtgcaccgc
    30841 cggagttacc gcggttaatg gaagcatctg tctggataaa gttttccaga ccttcaagat
    30901 tcaacccgct gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc ccaaggccaa
    30961 atgggttacc gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag tcggcaatag
    31021 cgatttgcgt taatttgctc gggttttgaa tttgtaacag ggcgatatcg ctctggtcat
    31081 cgctaccaat cagttttgca tcaaactcgc gcccatcatt gagctgaata ctgattttct
    31141 gtgcctgatt aatcacatgg ttgttggtca gcacatagcc tttactggcg ttgatgatga
    31201 caccggaacc taaaccttcg aagggttgtg caggttgatc cggtaaatca tcaccaaaaa
    31261 actttttgaa ttcttccggg attttctgtc cctgactggc cgttccttcc acccgtacgc
    31321 tcaccactgc cggaagcact ttttccagca ttggagccag actggggaga ggggcctgat
    31381 cggcaacctg gcctggaatc gacgcgacgg cctgaaatga cgccgagaga gttaacccgg
    31441 cacttaacgc taatgcactc aacagctggg tttgtttttt cattattcct gctctcgtac
    31501 ctgaatgata agaaaagaga ttcaaaacag tttgatgtta ttgaattttc agacggctaa
    31561 caatgagaca ccagattaaa tgatagtcgg ggagggagag aagaggaggg cgcaatggct
    31621 gcgcccgaaa aataaattag tcgcgcttcg cgccagtacg cagcaggccg gatgcgcctt
    31681 cagaatagtc gcgaggcacc tgcaccggtg cctgatcgtt gctggcttca gactctgcca
    31741 gacgattacg gaacgggttt gcttcagcag acagttccgg cagcaggctg ctggagcttt
    31801 ttgccatgtg ctgatacagc tggcgatagt cgtgcgccat ggtatccagt aattccgcgc
    31861 tgcgggcaaa gtggctaacc agctcttcgc gatactcgtc cagttcagct ttattctttt
    31921 ccagttcgta ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa cgcatggcca
    31981 cagcaccaat aatgatgccg acgactaacc caattagcgc atattcccag gtcatgaaca
    32041 tctcccgttg tcttttgttt ccgtagggtg ttggcttcag gctccatgcc tgcggctgat
    32101 tatgccacta taaccgttaa ttccacagaa gtggaatccc gactgcatat cgcgtagtgt
    32161 agaacggcct ttttttcgtc aaccgtgaac aacggcgcac cgattattca aggaataaca
    32221 ataagatcat gcaaagcgtt accccaacat cgcaatacct gaaggcgctc aatgaaggca
    32281 gccatcaacc cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt atttatcaag
    32341 aactcatcaa tagcaggcca ccagccccca ggacgagtgg gctaatggcg cgggtcggta
    32401 agctgtgggg taaacgcgaa gacacaaagc atacgccagt gcgtggctta tatatgtggg
    32461 gcggtgtagg acgcgggaaa acctggctga tggacctttt ctatcaaagc ctgccgggag
    32521 agcggaaaca gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa gagctaactg
    32581 ccttacaggg gcagaccgat ccgctggaaa ttattgccga tcgctttaaa gccgaaactg
    32641 acgtgctctg ttttgacgaa ttttttgttt ctgatattac cgacgccatg ctacttggcg
    32701 gtctgatgaa agccctgttc acccgcggta ttaccctggt agcgacgtca aatattccgc
    32761 cggacgaact ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca atcgatgcca
    32821 ttaaacagca ttgtgatgta atgaacgtgg acgctggtgt tgattatcga ctgcgtacac
    32881 tcactcaggc gcatctgtgg ctttcgccac tcaacgatga aacccgggcg cagatggata
    32941 aactatggtt ggcgctggcg ggggtgaaac gagaaaattc accgacgtta gaaatcaacc
    33001 atcggccatt ggcgacaatg ggcgtcgaga accagacgct ggcggtttct tttactacgc
    33061 tgtgcgtcga cgcccgcagt cagcatgact atattgcgct ctcacgcctg tttcacacag
    33121 taatgttgtt tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg cggcgcttta
    33181 ttgcgctggt ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt gcagaagtgc
    33241 cgctgtatga aatttatcag ggtgagcggc tgaagtttga gttccagcgt tgcctgtcac
    33301 gtctgcaaga gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg ggttaaaacc
    33361 tgccacaaat cacaaaaagg ggtcgatctt tgaccccgac ttctctataa tcctgcgacc
    33421 ccacgttaca agaaagtttt tttcccaaaa ctttttgtgt gctggcatag gctattcgaa
    33481 ggggtaggtt tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt ttgggtgttc
    33541 accaacgtgt aactatttat tgggtaagct tttaatgaaa acttttacag ctaaaccaga
    33601 aaccgtaaaa cgcgactggt atgttgttga cgcgaccggt aaaactctgg gccgtctggc
    33661 tactgaactg gctcgtcgcc tgcgcggtaa gcacaaagcg gaatatactc cgcacgtaga
    33721 taccggtgat tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg gcaacaagcg
    33781 tactgacaaa gtgtactatc accacaccgg ccacatcggt ggtatcaaac aagcgacctt
    33841 tgaagagatg attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta aaggcatgtt
    33901 gccaaaaggc ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg cgggtaacga
    33961 gcacaaccac gcggcacagc aaccgcaagt tcttgacatc taatcgggat tataggcaat
    34021 ggctgaaaat caatactacg gcactggtcg ccgcaaaagt tccgcagctc gcgttttcat
    34081 caaaccgggc aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt acttcggtcg
    34141 tgaaactgcc cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg ttgagaaact
    34201 ggacctgtac atcaccgtta aaggtggtgg tatctctggt caggctggtg cgatccgtca
    34261 cggtatcacc cgcgctctga tggaatacga cgagtccctg cgttctgaac tgcgtaaagc
    34321 tggcttcgtt actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc tgcgtaaagc
    34381 acgtcgtcgt ccgcagttct ccaaacgtta attggcttct gctacggcag aaaacaattt
    34441 gcgaaaaaac ccgcttcggc gggttttttt atggctaaat tctgaatcag cgtaaaaaat
    34501 gggaagttgc tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac atcgccaata
    34561 agggtctaag tcaactattt cagactaaag cgcatctctt tttccccatt tccggcatcc
    34621 actcaccaca aaggtcgcaa aatctggtaa actatcatcc aattttctgc ccaaatgtcg
    34681 ggtattgctc attttttgtt tgattttcga acaaagagag tcgttcctta ttgggtaaca
    34741 caacttctga ctggccacct ggtggctggt agcagtaaaa attctgacta tacctggagg
    34801 ttttcatggc tgtcgctgcc aacaaacgtt cggtaatgac gctgttttcc ggtcctactg
    34861 acatctatag ccatcaggtc cgcattgtgc tggctgagaa aggtgtaagt ttcgagatcg
    34921 aacacgtgga aaaggacaat ccgcctcagg atctgattga cctcaacccg aatcagagcg
    34981 ttccgaccct ggtggatcgt gagctgaccc tgtgggaatc tcgcatcatt atggaatatc
    35041 tggatgagcg tttcccgcat ccgccactga tgcctgttta cccggtagct cgcggtgaaa
    35101 gccgtctgta catgcatcgc atcgaaaaag actggtacac gctgatgaac actatcatca
    35161 acggttcagc ttctgaagca gatgccgcac gtaagcaact gcgcgaagaa ctgctggcga
    35221 ttgcgccggt cttcggtcag aagccgtact tcctgagcga tgagttcagc ctggtcgatt
    35281 gctaccttgc tccgctgctg tggcgtctgc cgcaactggg catcgagttc agcggcccgg
    35341 gtgcgaaaga gctgaaaggc tatatgaccc gcgtctttga gcgtgactct ttccttgctt
    35401 ctttaactga agcagaacgt gaaatgcgtc tgggccggag ttaatctgta tggatttgtc
    35461 acagctaaca ccacgtcgtc cctatctgct gcgtgcattc tatgagtggt tgctggataa
    35521 ccagctcacg ccgcacctgg tggtggatgt gacgctccct ggcgtgcagg ttcctatgga
    35581 atatgcgcgt gacgggcaaa tcgtactcaa cattgcgccg cgtgctgtcg gcaatctgga
    35641 actggcgaat gatgaggtgc gctttaacgc gcgctttggt ggcattccgc gtcaggtttc
    35701 tgtgccgctg gctgccgtgc tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt
    35761 tgagcctgaa gctgcctacg atgaagatac cagcatcatg aatgatgaag aggcatcggc
    35821 agacaacgaa accgttatgt cggttattga tggcgacaag ccagatcacg atgatgacac
    35881 tcatcctgac gatgaacctc cgcagccacc acgcggtggt cgaccggcat tacgcgttgt
    35941 gaagtaatac aaaacaggcc caggcggcct gttttgtctt tttaatgact taataaaata
    36001 gcgtgattgt ggcaatcata ttcaccacga aaccgactgc catggggatc gctgtccgct
    36061 tcaccaccgc aaaaggcgaa acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa
    36121 tcggcgaaac cgcgcgcgct agcgtcgtgg caaaatgcat cggcataatc attacaaccg
    36181 ctggtacatg tagtccggct gcgatattcg gaataagact ggcaaatgac ataaacggcg
    36241 cattgccaga gcccatcaca atggcacaaa tggcaatgac tagcgccata ataatcatca
    36301 cgccaatacc gcccagacca gaatgctccg cacccctgat aaccgcatca acagtgccaa
    36361 tcgtcgttaa gcctttcgca aatatttcac ccgcgaccac cagcgttacc acgttgacaa
    36421 actgcgtacc catgccgtca aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat
    36481 gcttgcggaa gaactcaaca aacatcgtca ccgtcaaact tagtaacatc accaccacca
    36541 gatgcagttc tgactgcatc agcccgacgt gggcgaggaa cagcgagccg agcatcagga
    36601 ttaacggcat cacaggtaaa atggcgtaat agagcggcgg gacattatcg agagtttttt
    36661 gctctgcctg ttcgtgattg atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa
    36721 a
//
LOCUS       DAJTYL010000027        36111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYL010000027 DAJTYL010000000
VERSION     DAJTYL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.027"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..890
                     /gene="hisG"
                     /locus_tag="OO572_001333"
     CDS             <1..890
                     /gene="hisG"
                     /locus_tag="OO572_001333"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6660396.1"
                     /translation="TRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAENMPI
                     DILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFGGCRL
                     SLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPRAGLA
                     DAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQGVIQ
                     ARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWETMEK
                     LKALGASSILVLPIEKMME"
     gene            896..2200
                     /gene="hisD"
                     /locus_tag="OO572_001334"
     CDS             896..2200
                     /gene="hisD"
                     /locus_tag="OO572_001334"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6660397.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2197..3267
                     /gene="hisC"
                     /locus_tag="OO572_001335"
     CDS             2197..3267
                     /gene="hisC"
                     /locus_tag="OO572_001335"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6660398.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3267..4334
                     /gene="hisB"
                     /locus_tag="OO572_001336"
     CDS             3267..4334
                     /gene="hisB"
                     /locus_tag="OO572_001336"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6660399.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4334..4924
                     /gene="hisH"
                     /locus_tag="OO572_001337"
     CDS             4334..4924
                     /gene="hisH"
                     /locus_tag="OO572_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6660400.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            4924..5661
                     /gene="hisA"
                     /locus_tag="OO572_001338"
     CDS             4924..5661
                     /gene="hisA"
                     /locus_tag="OO572_001338"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6660401.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5643..6419
                     /gene="hisF"
                     /locus_tag="OO572_001339"
     CDS             5643..6419
                     /gene="hisF"
                     /locus_tag="OO572_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6660402.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6413..7024
                     /locus_tag="OO572_001340"
     CDS             6413..7024
                     /locus_tag="OO572_001340"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6660403.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7210..8187)
                     /gene="wzzB"
                     /locus_tag="OO572_001341"
     CDS             complement(7210..8187)
                     /gene="wzzB"
                     /locus_tag="OO572_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6660404.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8333..9453)
                     /gene="ugd"
                     /locus_tag="OO572_001342"
                     /pseudo
     CDS             complement(8333..9453)
                     /gene="ugd"
                     /locus_tag="OO572_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9703..11109)
                     /gene="gndA"
                     /locus_tag="OO572_001343"
     CDS             complement(9703..11109)
                     /gene="gndA"
                     /locus_tag="OO572_001343"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6660405.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11218..11577)
                     /locus_tag="OO572_001344"
     CDS             complement(11218..11577)
                     /locus_tag="OO572_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660406.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(11574..12080)
                     /locus_tag="OO572_001345"
     CDS             complement(11574..12080)
                     /locus_tag="OO572_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660407.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12226..12789)
                     /locus_tag="OO572_001346"
     CDS             complement(12226..12789)
                     /locus_tag="OO572_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660408.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(12996..13934)
                     /locus_tag="OO572_001347"
                     /pseudo
     CDS             complement(12996..13934)
                     /locus_tag="OO572_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(13997..14371)
                     /locus_tag="OO572_001348"
     CDS             complement(13997..14371)
                     /locus_tag="OO572_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6660409.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14375..15523)
                     /gene="wzy"
                     /locus_tag="OO572_001349"
     CDS             complement(14375..15523)
                     /gene="wzy"
                     /locus_tag="OO572_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6660410.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15543..16448)
                     /gene="rfbG"
                     /locus_tag="OO572_001350"
     CDS             complement(15543..16448)
                     /gene="rfbG"
                     /locus_tag="OO572_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6660411.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16441..17331)
                     /locus_tag="OO572_001351"
     CDS             complement(16441..17331)
                     /locus_tag="OO572_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6660412.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17361..18617)
                     /gene="wzx"
                     /locus_tag="OO572_001352"
     CDS             complement(17361..18617)
                     /gene="wzx"
                     /locus_tag="OO572_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6660413.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18633..19178)
                     /gene="rfbC"
                     /locus_tag="OO572_001353"
     CDS             complement(18633..19178)
                     /gene="rfbC"
                     /locus_tag="OO572_001353"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6660414.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19183..20061)
                     /gene="rfbA"
                     /locus_tag="OO572_001354"
     CDS             complement(19183..20061)
                     /gene="rfbA"
                     /locus_tag="OO572_001354"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6660415.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20120..21019)
                     /gene="rfbD"
                     /locus_tag="OO572_001355"
     CDS             complement(20120..21019)
                     /gene="rfbD"
                     /locus_tag="OO572_001355"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6660416.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21019..22104)
                     /gene="rfbB"
                     /locus_tag="OO572_001356"
     CDS             complement(21019..22104)
                     /gene="rfbB"
                     /locus_tag="OO572_001356"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6660417.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(22477..23370)
                     /gene="galF"
                     /locus_tag="OO572_001357"
     CDS             complement(22477..23370)
                     /gene="galF"
                     /locus_tag="OO572_001357"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6660418.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23545..24939)
                     /gene="wcaM"
                     /locus_tag="OO572_001358"
     CDS             complement(23545..24939)
                     /gene="wcaM"
                     /locus_tag="OO572_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6660419.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(24950..26170)
                     /gene="wcaL"
                     /locus_tag="OO572_001359"
     CDS             complement(24950..26170)
                     /gene="wcaL"
                     /locus_tag="OO572_001359"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6660420.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26167..27447)
                     /gene="wcaK"
                     /locus_tag="OO572_001360"
     CDS             complement(26167..27447)
                     /gene="wcaK"
                     /locus_tag="OO572_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6660421.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27627..29105)
                     /gene="wzxC"
                     /locus_tag="OO572_001361"
     CDS             complement(27627..29105)
                     /gene="wzxC"
                     /locus_tag="OO572_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6660422.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29107..30501)
                     /gene="wcaJ"
                     /locus_tag="OO572_001362"
     CDS             complement(29107..30501)
                     /gene="wcaJ"
                     /locus_tag="OO572_001362"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6660423.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30556..31926)
                     /gene="cpsG"
                     /locus_tag="OO572_001363"
                     /gene_synonym="manB"
     CDS             complement(30556..31926)
                     /gene="cpsG"
                     /locus_tag="OO572_001363"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6660424.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32119..33555)
                     /gene="cpsB"
                     /locus_tag="OO572_001364"
     CDS             complement(32119..33555)
                     /gene="cpsB"
                     /locus_tag="OO572_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6660425.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33558..34781)
                     /gene="wcaI"
                     /locus_tag="OO572_001365"
     CDS             complement(33558..34781)
                     /gene="wcaI"
                     /locus_tag="OO572_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6660426.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(34778..35257)
                     /gene="gmm"
                     /locus_tag="OO572_001366"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(34778..35257)
                     /gene="gmm"
                     /locus_tag="OO572_001366"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6660427.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35257..>36111)
                     /gene="fcl"
                     /locus_tag="OO572_001367"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35257..>36111)
                     /gene="fcl"
                     /locus_tag="OO572_001367"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6660428.1"
                     /translation="LNLLDSRAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQ
                     NMMIESNIIHAAHQNDVNKLMFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAK
                     IAGIKLCESYNRQYGRDYRTVMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPD
                     VVVWGSGTPMREFLHVDDMAAASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIREL
                     AQTIAKVVGYKGRVVFDASKPDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLE
                     NQDRFRGE"
BASE COUNT         9707 a         9042 c         8095 g         9267 t
ORIGIN      
        1 acactcgttt acgcatagct atgcagaaat ccggccgttt aagtgatgac tcacgcgaat
       61 tactggcgcg ctgtggcatt aaaatcaatc ttcacaccca gcgcctgatc gcgatggcag
      121 aaaacatgcc gattgatatt ctgcgcgtgc gtgacgatga cattccctgt ctggtaatgg
      181 acggcgtggt agaccttggg attatcggcg aaaacgtgct ggaagaagag ctgcttaacc
      241 gccgcgccca gggtgaagat ccgcgctact ttaccctgcg tcgtctggat ttcggcggct
      301 gccgcctttc gctggcaacg ccggttgatg aagcctggga cggtccgctc tccttaaacg
      361 gtaaacgtat cgccacctct tatcctcacc tgctcaagcg ttatctcgac cagaaaggta
      421 tctcttttaa atcctgctta ctgaacggtt ctgtcgaagt tgcgccgcgt gccgggctgg
      481 cggatgcgat ttgcgatctg gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg
      541 aagtcgaagt tatctaccgc tcgaaagcct gcctgatcca gcgcgatggc gaaatggaag
      601 aatctaaaca gcaactgatc gacaagctac tgacccgtat tcagggtgtg atccaggcgc
      661 gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg cctggatgaa gtcatcgccc
      721 tgctgccagg tgccgaacgc ccaaccattc tgccgctggc gggtgatcaa cagcgcgtag
      781 cgatgcacat ggtgagtagc gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcac
      841 tgggtgccag ttcaatcctg gtcctgccga ttgagaagat gatggagtga tcgccatgag
      901 ctttaacaca atcattgact ggaatagctg tactgcggag caacaacgcc agctgttaat
      961 gcgcccggcg atctccgctt ctgaaagcat tacccgcacc gttaacgata ttctcgataa
     1021 cgtgaaaacg cgtggcgatg aggccctgcg tgaatacagc gcgaagtttg ataaaaccac
     1081 ggttaccgcg ctgaaggtgt ctgctgatga gatcgccgcc gccagcgaac gcctgagcga
     1141 cgagctaaaa caggcgatgg cggtggcagt aaagagtatt gaaaccttcc acactgcgca
     1201 aaaactgccg ccggtagatg tagaaacgca gctaggcgtg cgttgccagc aggtcacgcg
     1261 cccggtggct tcagtgggtt tgtatattcc gggcggctcc gccccgctct tctcaacggt
     1321 attaatgctg gcaactccgg cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc
     1381 gccgccgatt gccgatgaaa ttctttatgc ggcgcagctg tgcgatgtgc aggacgtgtt
     1441 taacgtcggc ggcgcacagg ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa
     1501 agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc gaagcaaaac gccaggtaag
     1561 ccagcgtctg gacggtgcgg cgatcgatat gcccgcaggc ccgtcggaag tgctggtgat
     1621 tgctgacagc ggcgctacgc cggattttgt ggcttctgat ttgctttctc aggctgaaca
     1681 cggccctgac tcacaggtaa ttttactgac ccccgatgct gatatggcgc gtcgcgttgc
     1741 cgaggccgtc gaacgccaac tggcagaact gccgcgtgcc gaaaccaccc gccaggcact
     1801 gaacgccagc cgcctgatcg tgactaaaga tttagcgcag tgcgtggaga tctccaacca
     1861 gtacggcccg gagcacctga tcattcagac ccgcaacgcc cgcgaactgg tcgatggcat
     1921 caccagcgcc ggttcggtat ttcttggtga ctggtcaccg gaatcggcag gcgactacgc
     1981 ctccggcacc aaccacgtgc tgccgactta cggttacacc gccacctgtt ccagcctcgg
     2041 gctggcagat ttccagaaac gcatgaccgt acaggaactg tcgaaagaag gtttctccgc
     2101 gctggcttcg accattgaaa cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc
     2161 cgttactttg cgtgttaacg tccttaagga gcaagcatga gcaccgtgac tattactgat
     2221 ttagcgcgtg aaaacgtccg caacctgaca ccgtatcagt cggcgcgtcg tctgggcggt
     2281 aacggcgacg tctggctgaa cgccaacgaa tatcccacag ccgtggagtt taagcttact
     2341 cagcaaacgc tcaaccgcta cccggaatgc cagccaaaag cggtgattga aaattacgcg
     2401 caatatgcag gcgtaaaacc ggaacaggtg ctggtcagcc gtggcgcgga cgaaggtatt
     2461 gaactgctga tccgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta ctgcccgcca
     2521 acgtacggca tgtacagcgt cagcgctgaa accattggtg tcgaatgccg cacagtgccg
     2581 acgctggaaa actggcaact ggacttacag ggcatttccg acaagctgga cggcgtaaaa
     2641 gtggtctatg tttgcagccc caacaacccc accgggcaac tgatcaatcc gcaggatttt
     2701 cgcaccctgc tggagctaac ccgcggtaag gcgattgtgg ttgccgatga agcttatatc
     2761 gagttttgcc cgcaggcatc gttagctggc tggctggcgg aatatccgca cctggcgatt
     2821 ttgcgtaccc tttcgaaagc cttcgcgttg gcggggcttc gttgcggatt tacgctggca
     2881 aacgaagaag tcatcaacct gctgatgaaa gtgatccccc cctacccgct ctcgacgccg
     2941 gttgccgaca ttgccgcaca ggcgttaagc ccgcagggaa tcgtcgccat gagcgaacgg
     3001 gtggcgcaaa ttattacaga acgcgaatac ctgattgccg cactgaaaga gatcccctgc
     3061 gtggagcagg ttttcgactc tgaaaccaac tacattctgg cgcgctttaa agcctccagt
     3121 gcagtgttta aatctttgtg ggatcagggc attatcttac gtgatcagaa taaacaaccc
     3181 tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt
     3241 gacgccttac gtgcggagca agtttgatga gtcagaagta tctttttatc gatcgcgatg
     3301 gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct
     3361 ttgaaccggg cgtgatcccg gaattgctga agctgcaaaa agcgggctac aagctggtga
     3421 tgatcactaa tcaggatggt ctgggaacac aaagtttccc gcaagcggat tttgatggcc
     3481 cgcacaacct gatgatgcag atcttcacct cgcaaggcgt gcagtttgat gaagtgctga
     3541 tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta aaactggtgg
     3601 agggttatct ggctgagcaa gcgatggatc gtgccaacag ttatgtaatt ggcgatcgcg
     3661 cgaccgacat tcaactggcg gaaaacatgg gcattaatgg tttacgctac gaccgcgaaa
     3721 ccctgaactg gccaatgatt ggcgagcaac tcactaaacg agaccgttac gcccatgtag
     3781 tgcgcaacac caaagagacg cagattgacg tccaggtgtg gctggatcgc gaaggtggca
     3841 gcaagattaa caccggcgtt ggcttctttg atcacatgct ggatcagatc gccacccacg
     3901 gcggtttccg catggaaatc aatgtcaaag gcgacctcta tatcgacgat caccacaccg
     3961 tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat tgccctcggc gacaaacgcg
     4021 gtatttgccg ctttggtttt gtgctgccga tggacgaatg ccttgcccgc tgcgcactgg
     4081 atatctctgg tcgcccgcac ctggaatata aagccgagtt tacctaccag cgtgtgggcg
     4141 atctcagcac tgaaatgatc gaacacttct tccgttcgct ctcttacacc atgggcgtga
     4201 cactacacct gaaaaccaaa ggtaaaaacg atcatcaccg tgtagaaagc ctgttcaaag
     4261 cctttggtcg taccctgcgc caggccatcc gcgtcgaagg cgacaccctg ccctcgtcga
     4321 aaggagtgct gtaatgaacg tggtgatcct tgatactggc tgcgccaacc tgaactctgt
     4381 gaagtctgcc attgcgcgcc acggttatga acccaaagtc agccgtgacc cggacattgt
     4441 gttgctggcc gataaactgt ttttacccgg cgtcggcacc gcacaagcag cgatggatca
     4501 ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt acccaaccgg tgctgggcat
     4561 ctgcttaggg atgcaactcc tgggacgacg cagcgaagag agcaatggcg tcgacttgct
     4621 gggcatcatc gacgaagacg tgccgaaaat gaccgacttt ggtctgccac tgccacatat
     4681 gggctggaac cgcgtttatc cgcaggcagg caaccgcctg tttcagggga ttgaagacgg
     4741 cgcgtacttt tactttgttc acagctacgc aatgccagtc aatccgtgga ccatcgccca
     4801 gtgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct acggcgtgca
     4861 gttccacccg gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact tcctggagat
     4921 gtgatgatta ttccggcatt agatttaatc gacggcaccg tggtgcgtct ccatcagggc
     4981 gattacggca aacaacgcga ttacggtaac gacccgctgc cgcgcttaca ggattacgct
     5041 gcgcaaggtg ccgaagtgtt gcacctggtg gatctgaccg gggcaaaaga tccggctaag
     5101 cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta acgtcccggt gcaggttggc
     5161 ggcggtgttc gtagtgaaga agacgtggcg gcgttactgg aagctggcgt tgcgcgcgtg
     5221 gtggtcggtt ccaccgcagt gaaatcaccg gacgtagtga aaggctggtt tgaacgtttc
     5281 ggtgccgatg cgctggtgct ggcactggat gtccgtattg acgagcaagg caacaagcaa
     5341 gtggcagtca gcggctggca agagaactcg ggcgtttcac tggaacaact ggtggaaacc
     5401 tatctgcccg tcggcctgaa acatgtgttg tgtaccgata tctcgcgcga cggcacgctg
     5461 gcgggatcta acgtctcttt atatgaagaa gtgtgtgcca gatatccgca agtggcgttt
     5521 cagtcctccg gcggcattgg cgacattaac gatgttgctg ccctacgtgg cactggcgtg
     5581 cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat tcaccgtgaa ggaggccatc
     5641 gcatgctggc aaaacgcata atcccgtgtc tcgacgttcg tgatggtcag gtggtgaaag
     5701 gcgtacagtt tcgcaaccat gaaatcattg gcgatatcgt gccactggca aaacgctacg
     5761 ccgaagaagg tgcagatgaa ctggtgttct acgatatcac cgcttccagc gatggccgtg
     5821 tggtagataa aagctgggta tctcgcgtgg cggaagtgat cgacattccg ttttgtgtgg
     5881 cgggtgggat taagtctctg gaagatgccg cgaaaattct ttcctttggc gcggataaaa
     5941 tttccatcaa ctcccctgcg ctggcagacc caacattaat tactcgcctg gccgatcgct
     6001 ttggcgtgca gtgtattgtg gttggtattg atacctggta cgacgccgaa accggtaaat
     6061 atcatgtaaa tcaatatacc ggcgatgaaa gccgcacccg cgtcactcaa tgggaaacgc
     6121 tcgactgggt agaggaggtg caaaaacgcg gcgccggaga aatcgtcctc aacatgatga
     6181 atcaggacgg cgtgtgtaac ggttacgacc tcaagcagct gaaaaaagtg cgtgaagttt
     6241 gccacgtccc gctgattgcc tccggtggcg cgggcaccat ggaacacttc ctggaagcct
     6301 tccgcgatgc cgacgttgac ggtgcgctgg cagcttccgt attccacaaa caaataatca
     6361 atattggcga attaaaagcg tacctggcaa cacagggcgt ggagatcagg atatgttaac
     6421 agaacaacaa cgtcgcgaac tggactggga aaaaaccgac ggtctgatgc cggtgattgt
     6481 gcaacacgcg gtatctggcg aagtgttaat gctgggctac atgaacccgg aagccttaga
     6541 caaaaccatc gaaagcggta aagtcacctt cttctcgcgc actaaacagc gactgtggat
     6601 caaaggcgaa acctcgggca attttctcaa tgtggtaagt attgccccgg actgcgacaa
     6661 cgacacatta ctggtgctgg cgaatcccat cgggccaacc tgccacaaag gcaccagtag
     6721 ctgcttcggc aacaccgctc accagtggct gttcctgtat caactggaac aactgctcgc
     6781 cgaacgtaaa tatgccgacc cggaaacctc ctacaccgcc aaactgtatg ccagcggcac
     6841 taaacgcatt gcgcagaaag tgggcgaaga aggcgtggaa accgcactgg cagcaacggt
     6901 acatgatcgc tttgagctga ccaacgaggc atctgacttg atgtatcacc tgctggtgct
     6961 gttgcaggat caggatctgg atttaacgac agtaattgag aacctgcata aacggcatca
     7021 gtgagttacg ggctgagcag gtgtgatgtt gttgtcacac ccggagccat tgcccgatgc
     7081 gcaaacttat cgtgcctaca cattatctca acacacgtag gccggataag atgcgccagc
     7141 atcgcatccg gcaaaaaaaa cgggcatggt gtcaccaccc tgcccgtttc tcttaaatgc
     7201 acaataatat tacttcgcgt tgtaattacg cagagcgtta cgccccaaca caatccccgc
     7261 gccaaccatg ccgcccagaa gcactgccag aatcaaggtg attgcctttt tcggactatc
     7321 gcgacgaata ggtaacgtcg gtttcatcac atagcggtaa gcatgaatat caagatcatc
     7381 aaactttaat ttttcaatat ccaacaggtt ttgacgtgtc tgatagtagt ttggtgagaa
     7441 caccaacgga cgagtcgctt catgcttaat catcgactcc agcgcttcgc tccctagaag
     7501 gaacaacgta tcttgcgtca catcttcagt ctgctgaacc tgtggctttg tcacctgcgc
     7561 ctgattcgca tactgcaacg cttcctgaat ctgacggata cgcagatctt tctgctcctg
     7621 cgcgaccact tcctgggttc ttaaagagtc ctgcaagttt ttccgtccca gagcaatgtt
     7681 gtccttgaga tccttttcta gctcttgatt cactttatca tcaacttgct gaatgtattg
     7741 ggccaacttc atttgtgcgc cctctgcagt ttgcccaaca taagaaacag tcaatggtaa
     7801 ttgctggttc ttaacagaag gttcgatggt aagtttttct ggctcttcct gattatccag
     7861 cgtttctgct aatgcagaga aggcagaact gaagcgacca attaacgtct cctgcaaatc
     7921 cgatactttc ggtgcagcct gaccatagat aacattcatg gcattgttat agccagcaat
     7981 ttgccccacg tcgggctgag tgataattgc tgttgacgtc catttctcct tcgctactgc
     8041 caaataacca atagccaggg caatagccac aatgacggaa atgataattg tcatcttgcc
     8101 acgccacaac tgcactagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc
     8161 agaaacatta ttattttcta ctctcatcat taccctaact gacggaaatt tccaagtccg
     8221 catagtttag cgataatccc cgtgaatgct ataggataag tgataaaaat aatgaattat
     8281 tgcggaggta acaaaagaca atcagggcgt aaatagccct gataacaaga tgttagtcgc
     8341 tgccaaagag atcgcgggtg tagaccttat ctgccacatc cttaagctct tctgccatac
     8401 ggttggagat aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccaggcgag
     8461 agttgaagaa tgagtcttct ttcatcaccg gctcgtagat gatcacttca acgcctttcg
     8521 ccttgatacg cttcataatc ccctgaatag aagacgcacg gaagttatct gacccgctct
     8581 tcataatcag acgataaata cccaccactt gcggtttgca tcgacaattg ccgagatcag
     8641 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     8701 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     8761 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     8821 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     8881 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     8941 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9001 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9061 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9121 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9181 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
     9241 gtaggcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
     9301 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
     9361 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
     9421 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
     9481 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
     9541 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
     9601 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
     9661 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
     9721 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
     9781 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
     9841 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
     9901 cgcctgctgg tagtcatcgg caatttgctt gaagtacggg gccagcagca ggttagcgat
     9961 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10021 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccaattgt attcttcaga
    10081 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10141 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10201 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    10261 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    10321 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    10381 atcttttttg gtgaagatat ctttagtgat gtcgatcagg tagctgctaa gttcaccgtt
    10441 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    10501 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    10561 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    10621 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    10681 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    10741 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    10801 ggtattacca ccatcaatga tgatgtcacc tttatcgaga tatggtttca gggaatcaat
    10861 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    10921 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    10981 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11041 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11101 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11161 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    11221 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    11281 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    11341 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    11401 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    11461 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    11521 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    11581 ttcagaataa aaatatttga attcaaaata caacgatcgc cccgaaaaaa aataatacac
    11641 acaaggataa aatatcgtcc caacttgtaa tttgtaatgt tgatatcttt tattaaactt
    11701 gcaattgaga tcgcaacctg caatactgac aatgggtaaa tgggtgataa atacctgtca
    11761 ccttgcgtaa cagaaaacca aaataagaca tatgttaaaa caatcgagaa aaatacccaa
    11821 agtattttat tattcctaag tgtaaagaca gacagtatag cataaaaaac aacatcactg
    11881 ccaactttat taattgcttg ttgcaaatct aatgtggcag gacttatacc atgtcgagct
    11941 tgttctgctg tttgaacttg tagatcaatt tcattccata ggtagtgacc aaatatattt
    12001 cctccagcag gactaaaagg atcaccagag atccaaaaac tacggacata ccaataaatg
    12061 ccaccaagaa ttgttgccaa gctaaaaata agaaatgtct gtatgtttct ctttttcgct
    12121 atcaatataa caaatatgat ggctccatat atggcaccaa atagttttac gccacaagca
    12181 attccaataa aaaaaccaca taaaaaaatt aattcatttt gtgttttatt gttttctgag
    12241 cgaatatata taagaatact ggctgcagca aaacaaagat agcgttaaat caatatatgc
    12301 ataaccaaga tgatctttaa gtggattgat agtaaatagt aggattgtag caataatgcc
    12361 tggataataa gttttcgtaa tttcccatga acagccaaca agtccacagg ctattacaaa
    12421 aaaacagata tttgctaaaa actgagctaa ccttacatct cctaactgta aacctaaagc
    12481 cattaaaaga tctgcatttt gcgggaacag aggaaaacgc aaatattgtt caacaacaat
    12541 tgcatggtgc tcaactaatg aacgagccaa aggtaagtga tacatagtat catcccaatc
    12601 accgggagct cgcagggacg ataacaatgt aaccacaaaa atcaaaacaa gaaataaaat
    12661 aacaaacagc gtactgaact caatattatt tattgccagc catctaaatt gctgacgttt
    12721 aataatgctt aaaacccaac acaccgtaaa aattaaaaag atagcaatct tattaaattg
    12781 cccgaccatg cccagaaagt aaattatcaa aatatttaaa gacatcccta tgcataggca
    12841 ataaaaaata aagaaaattt gttaagtcct gccaccccct ccttaaaaaa agatgacaat
    12901 acaatattac caatgacaaa aaatgtcatt ccaaaagcaa aacaatacac aacatacaaa
    12961 tacaacggca attcaatcat agtaaaatac tctatttaca ttatcagttt atagtttttt
    13021 tcagaaagat aaaataatgc cttcatttca cgtctagttg ttgtatttga atgcaaaata
    13081 atgccacata caaatgccat tattgacgat aacatgatgg aggcagttaa aaaggcagtc
    13141 ggaaatcttc tcaccaaacc tgtatcaata taatcaacaa tgatgggcaa tcctaaaatt
    13201 atagacaaag cagcaagtaa aacgccgata atactaaaaa agtatagtgg ccgttcaagc
    13261 gagtaaagtt taattattgt tttcagaatc ttgaacccat cactccaagt tgatagtttg
    13321 ctcacactcc cttcagatcg ctcgccatat tttgtattaa cctcaccata cttcatacgt
    13381 aattctaaag catgaattgt aagctcagtt tcgatttcaa atccccttga tagacatgga
    13441 aaagatttaa catagcgtct ggaaaataca cgataccccg acaacatatc agtaaaattc
    13501 cctttaaaga tagtctgaac agtaccagtc aacaatttat ttcctcagcg atgccctttt
    13561 ctgtatgtat tttgatctcc attctcttga cgacatccta caaccatatc caattgattt
    13621 tttatgagat ggttaatcat ttctggagca gaacttgcat cataagtgtc atctccatca
    13681 accattagat aaatatccgc atctacatct gagaacatac gtcttacgac attccccttg
    13741 cctttcagag gaacagagtg aactgttgct ccagcatcct ctgccacttg tgctgtactg
    13801 tcagtcgaat tattatcaaa tacatatata gaagcagttg gaatattcgt tttgaaatca
    13861 tttatgactt ttgctattgc cttctgctca ttataacaag gaataattac agcgatatta
    13921 aaagagtgat acataagccc ctcatataaa ctatttagct aatgtgcaag taattgtgtt
    13981 tttaacaaat cgtttactaa cgaaaaatga aatattttga tgcgcaatat cctaaaacca
    14041 aactaaaaag agaaaaggtg aacaatgtaa atattggaga taaagctaag agatcaaata
    14101 aaacgccgaa taaataactc atcgccccca taaaaaatat aaatataaaa tatctagcct
    14161 tggttgcaga tactttaaat gagcatcttg catttgcaaa aaaagaaaag gttgctgcac
    14221 atataaaacc aacaatgtta cttcggcctt gaccaaaagc taaattatta taacacgcat
    14281 aaaatacccc ccaatgtaaa gcagtattga caattccaat taaaaaataa ctaaaaaaag
    14341 aactggtcaa taacttccct atttttaaca tcctttattt tgctccagaa gtgaggttat
    14401 tactaatttg gatattttct atagaaaata cccataaaac tacaacgcct ataataaaaa
    14461 ttcccatggg tgtaaaaaga aatggattag ttatagaaac ggtaatactg aaaaaaagta
    14521 ttaacgacat cattaatgat gtttttgtac tatttaaaaa atagcctttc aaaaagataa
    14581 gcaacatagg aactaatgat ataaatacac caataacgcc tgttttctga agtatctcaa
    14641 gaggtactat ttcaatcctt aatcgatcta atataaatga accaaatcct cttccaaaca
    14701 agaacgtcgc taaatcaaca tttttataat aaaaatataa atcattaaat ctaacagagt
    14761 cagaatcttc actgcgtgat ccgaccatgt aaagcataat tataaataga acgctgccca
    14821 atattattat aatagtattt aaatatagct tatgtaattt aaaactggct aataacagtg
    14881 ataatgttgt aaaaagtaat acacctcttg attctgtcaa taatatcgca agcactatta
    14941 tgagacattt taaatatttt ttgtcgacaa aactgataat cgcaccgaca ccaaaaaagt
    15001 agaaggattt ggaaaaaaaa gccccatcgg gacgaaaaaa gaactcgcta gtcaatgata
    15061 agtgttcata aattaaatta aaatttaaca aaccgaaatt caacaaaatt atgtatgaaa
    15121 tatataacag tgatccatat aatgcaaatg cattaactat tttaataaaa atctcacatg
    15181 aaaatacttt taaagtatat cttagtgtaa atagatatat aagtgacagc ataaaaaggt
    15241 atggttgcac atcaaacagt atatcattta ttttattacc agctaataag ccgacaaaga
    15301 agccataaaa aggaaataaa agcaaccata taaaaagact gacacatttt tgggtgattg
    15361 aaaaattgaa tgtaactaaa ttaaaaatta aaagtataaa actaaataca aataataaat
    15421 atcgcaaagg gaatattccc aatggctcca gtaatcttcc accaccacca ataatcagtt
    15481 caatacataa aaatgttata aaaattttat ttatattatt catattcgta aggtgatgtt
    15541 ttttaaaata tgtttaaaat caacattaat aaaatataaa ttttatcagc ctttttccgc
    15601 cttaacttcc aaaaacttaa agccaagatc aaattgccta tataaccttt acccaattga
    15661 ttattcacaa agctatcatc agctaaggca ttagctattt tagttacttc ttttcgatac
    15721 cacttgtttc tcaccaaacc aagccgtttg tattttgctt taaaactaat gtttgctccc
    15781 acttggttgt tttcatgctg tctgtacagc attgttggtt ttctatcaat ataccacgaa
    15841 taatttctcg tccgcgcaaa tgaatataaa aaccaatcat gcagacagat atcttgtgca
    15901 gcatttttat tatttatgat gaacttctta aattcaataa gcgtttcttt ttttataaca
    15961 tatgtacacc ccggacctgc tgcttcaaaa aaataatcga attgagtttg cgactgagcc
    16021 ttatcaacta aattttttcg ccctgatgga taatatgcaa ttacattgct ggaataacaa
    16081 ttcgcattat taaataccaa gcagtcaatt gcatttttta ttttatcttt ataccaaata
    16141 tcgtcctgat cactaaaaca gatataatca tagttttcaa gatcgacatc tttaattaat
    16201 ctataaaaat ttttacctgc ccccccaaac ttattgacgc ttggaagtat atttataatt
    16261 gagggatatg ataattgaaa ttcttcacaa atatttaatg tattatcagt tgataaatca
    16321 tctgatataa aaattgagat gtctacatct ttttgttcaa taatactttt taattgttcc
    16381 tcaatccagc attcgccatt atacgtagcc ataagtacag ctactctttt ttgatcagaa
    16441 ctatgcatta taacgaccgc ccccagtaat tcctcttatt ccatccagcg tagccaaaat
    16501 atgttttttc atatattcaa ttttattttt ttgagttgcg ataattaaag tcgtatgaat
    16561 taaagaaaaa acaacttccc taatagagag taaaagaggc acatgatttt ttctaagcaa
    16621 aagaaatgca tttcttactc gataataacg acgtactggc gagtgaattg gtatgttaat
    16681 cccacagatt tttactgact tatccccaat agcatgaacc atttctatat caggaatgat
    16741 atgaactaat atgccatagt ttaagcatct aagacaccat tctgtatcga cataatcaat
    16801 aaaaagttcc tcatccatca ttccaacaat ctcaaaaact tcttttgaaa ccatggttcc
    16861 ggatgagatt gttactgaac ttttaaacgg tgtctgtcct tcagtgacat gaattttctc
    16921 tcgaagacca ttttttttga tattacaaat aggataataa tgtgacttat ctctatcaat
    16981 aaaaactggg ccagctattt ttgcattttc attatccata tagcaggaaa gttttttgat
    17041 aaactcattg cgaatacggc tatcttgatc aaaaaaaatt atcttctctg cccctttttc
    17101 aagagaatat cgaatcccaa tattttgtgc ttttgctata ccttcattcc taccaagaca
    17161 aattaaatga actttttcaa tgtctgctaa tttatctttc aaggtgaaat tagtcctatt
    17221 atcgactact acaacgtaac aattttgctc ctttagttca gtgatcagtg cattcagatt
    17281 tttaagttcg ggattatatg tcacaatgac agcgtaaata ttactattca ttatccaagt
    17341 gactcagtaa ttggttaatt ttagttttca tatacagaac acattctagt ataaagccat
    17401 tgtaataaca gagcatctaa aattacacgt atgctccagg atattgcggc acccagtaac
    17461 ccatattttt cgaccataaa atacaaaagg gctaaataag gaaccaattc agcacaatga
    17521 atcaaagcag taattttaga ttttcctaat gcctggattg cagagaatgg tatcattgcc
    17581 agcgcattga agaaaaaacc gatcaataac acatttaata tattaaagga attaatttga
    17641 taattctcac caaaccatat tttcaataca agccctgaat ataacaagcc tatgattatc
    17701 accggcaaac agattagaaa cattaaaagc aatgatttat ttacattacg tttaaattct
    17761 ttaaagtctt tgatgttact taaccttgga aacactgccc tcccgattgc tgcaggtatt
    17821 attcctaatt ttagaataac ctccgctggt gcagaataaa atgcaacttt atctgccccc
    17881 ataatatttg acactataaa tcgatcaaaa tacaccatga ccggacttat gatattactt
    17941 actgtcatcc aaccgccaaa gaaaaagagt cgtttaaagg ttttataaca aaaatgaacc
    18001 cccgaaattt taatatcatt cctgacataa taggcagaga ctaaaatcga aataactctt
    18061 gcaaatatta aaccagcaac cgctgccgac aatgtagcac tataaaaaac aaatatggcc
    18121 ggaattcccg cgatgcaaga gcttgatata gatttttgaa tatttacaat gccaaatttt
    18181 tcatccccct caagaatggc tgaccataat tgatttagaa taaataatgg aatgcaaata
    18241 gccaacagtt taaatgctag ttgtatatca ctatgttcaa caccagaaat ttttagataa
    18301 ttaacaattc catcagagaa aatcaataat aaaaaagcac caaagcatga aaatagcacc
    18361 aaaaaagatg tacttgttga aattaccttg gttctttcat gatgattatc acgatgaatt
    18421 gcaatttctc taataacaga gcgagtcagg cctacatcaa aaattccagc atacccaaca
    18481 agtgcaattg caattgtata aacaccgaaa ccttcaggcc ccaaacctcg agcgagaaat
    18541 cctagcgagg ggatagcaat taaagttggt attgcatagc caaaaaggtt ccagacacta
    18601 ttttttatta tgctcattat tacatttctt tatcatataa aataatcagc atctacaaat
    18661 ttttgaccat taatatcttt atcagaaagc aatggatttt gaaccggcca ttctatattt
    18721 atacttttgt cgctccataa aatcccacgc tcttgcattt ttgaatagaa attatttgtt
    18781 ttgtagacaa attctgcttc atcactaaga acgcaaaacc catgagcgaa tccttccggt
    18841 atccataatt gacgcttatt ctccgccgac aaatttacac caacccactt gccaaatgtt
    18901 ggtgatgatt tccgaatatc aacagctacg tcaaaaactt caccgacaac acagcgtacc
    18961 agttttcctt gagcataagg ttccagctga tagtgcagtc cgcgtaacac acccttagtt
    19021 gatttagaat ggttatcctg aacaaactca acctttcgtc ctacagcctc ttcgaaaact
    19081 ttctgattga agctttccat aaaaaagccc cgctcatcac caaatacttt tggttcaaaa
    19141 attagcacat caggaatttc agttttaatt acgttcattt tattaataac ctttaatcat
    19201 tttcagcaga tactgaccat aagcattttt cttcagcggc tcggctaata ccttcacttg
    19261 ctcagcatca ataaagcctt tacggtgagc aatttcttcc gggcaggaaa ctttcagccc
    19321 ctgacgctct tcaattgttg caatgaagtt gcttgcttca ataagacttt gatgtgtccc
    19381 cgtgtccagc catgcataac cacgccccat catagcgaca gacaaacgtc cttggtccat
    19441 ataaatacgg ttaatatcgg taatttccag ttcgccacgt gcagatggtt taaggttttt
    19501 cgccatttct acaacgtcat tgtcatagaa ataaagccca gtaaccgcat agttactttt
    19561 tggttcctgc ggtttttctt ccagactaat tgccgtaccg ttatcatcaa attccaccac
    19621 accgtagcgc tcaggatcat ttacgtgata ggcaaaaacc gttgcgccac tctctctgtt
    19681 aacagcggta tccattaatt tcggcaggtc gtgaccatag aagatattat caccaagtac
    19741 cagcgcacaa tcatcaccac ctataaattc ttcaccaatg ataaatgctt gcgccagacc
    19801 atccggactc ggttgtactt tatattgcag attaagcccc cactggcttc catcaccaag
    19861 caattgttga aaacgtggtg tatcctgcgg cgtactgata atcagaatat ctcgaatacc
    19921 cgccaacata agcgttgaaa gcggataata aatcatcggt ttatcataaa tcggtagcag
    19981 ttgtttgctc acagccattg tcacaggata aagacgagtg ccggaaccac cagccagaat
    20041 aataccttta cgcgttttca tttcaccatt ccttttaatt catctcgctc tagcatcatg
    20101 agcgagatgc aataatttgt taaattgccg tggtcgtaaa taattcgttg agcatacgtt
    20161 tcacgcccac ctgccagtca ggcaagacaa gcgcaaagtt ctgctgaaat ttttcggtat
    20221 tgaggcgaga attatgggga cgacgtgctg gtgtgggata ggccgttgtc ggcacggcat
    20281 taagtttgtt aagagcaagg ttgatccccg ctctgcgcgc ttcttcaaat accagagcgg
    20341 cataatcgtg ccaggttgtt gtaccaccag caaccagatg gtacaaacca gcaacttctg
    20401 gtttatttgc ggccacgcga atggcatgag cggtgcaatc agccagtaat tcagcccccg
    20461 ttggtgcgcc gaattgatcg tttattacag ccaactcttc gcgttctttt gccaggcgca
    20521 acatcgtttt ggcaaagtta tttcctttac cagcgtatac ccagctggta cggaaaataa
    20581 gatgctttgc gcaatgttct tgtaacgctt tttccccagc tagcttggtt tcaccgtaga
    20641 catttagcgg tgctgttgca tccgtttcca gccatggcgt gtcaccgttg cctgggaaaa
    20701 cataatcagt ggagtagtgt ataacccagg ctccgacttc attagctgct tttgcaatcg
    20761 cttcgacgca tgtcgcgtta agtaattgcg cgaaattcgg ttctgattcc gctttgtcta
    20821 ctgcagtgtg agcagcagca ttaacaataa catcggggcg aatttttttg acggtttcag
    20881 ccacaccttc ggggttgctg aaatctccac aataatcagt ggagtgaaca tcgagcgcaa
    20941 tcaagttacc cagaggagcc agagcacgct gcagttccca acccacctgc cctgttttgc
    21001 cgaaaagcag gatattcatt actgacggcc ctcatagttt tgttcaatcc acgactgata
    21061 agtaccgctt ttcacattct caacccaatt tgtattagcc agataccatt ccaccgtttt
    21121 acgaatcccg ctctcaaacg tttcttgcgg tttccagccc aattcgcggc taattttatc
    21181 ggcatcaatg gcgtaacggc ggtcatgccc cgggcgatcg gcaacataag taatttgctc
    21241 acgataagat ttctctttcg gtacaatctc atccaacaaa tcacaaatag tgagcactac
    21301 gtcgatgttt ttcttttcgt tatgtccacc aatgttataa gtttcacccg ctttaccttc
    21361 ggttacgacg gtatataacg cacgagcatg atcctctaca tacaaccagt cgcgaatttg
    21421 atccccttta ccataaatag gtaatgcctt accttccaga gcattcagaa taaccaatgg
    21481 aataagtttt tccgggaagt gataaggacc ataattgttc gagcaattag tcacaatggt
    21541 tggtaaacca taagtccgct tccaggcacg taccaaatga tcgctggaag ctttagacgc
    21601 agaatacggg ctacttggcg cgtaagctgt cgtttccgta aatagcggta acgcttcgtt
    21661 attatttgct tcatccggat ggggtaagtc accataaact tcatcagtag aaatatgatg
    21721 aaagcggaaa ctttttttct tttcatcatt cagagcagac cagtaattgc gcgccgcttc
    21781 taaaagaaca taagtcccca caatattggt ttcaataaat gccgcagggc ccgttattga
    21841 acggtcaacg tggctctctg ccgccaggtg catcaccgcg tctggctggt gctgtgcgaa
    21901 aatacgcgac atggctacag catcgcagat atctgcatgc tcaaaagcat aacgttcaga
    21961 atcagaaaca tcagcgagtg attccaggtt cccggcgtat gttaatttat cgacattaac
    22021 aacactatct tgcgtattat ttattatgtg acgaacaaca gcagaaccaa taaaccctgc
    22081 gccaccagta acaagtatct tcactttttt attccatata gctatagagc atgctgtgaa
    22141 atagactgct ccagatttga ttaatagagg tattaataca cgctaccgcc cctggcttaa
    22201 cagctaccag tgcactgcat gcatctcaac gatgtggcga gcgttagcca ctcgcgccaa
    22261 atccgaaaaa ttcaaacgtt aattgtctta ccaaacagcc gtagaaacaa ggaaaatcct
    22321 ggaaaaattt gaataatatg agctatgtaa cttcttgttt ttataggctt tataaaaacg
    22381 gcaacggcag gaaaattcaa tgagcaaaac ttatgccacg aatcttcact gccgttataa
    22441 ttttcttatc aaccgttaca tccggtcaga ttttcattat tcgcttaaca gcttctcaat
    22501 acctttacgg aacttcgccc cttctttcag gttgcgtagg ccatacttca caaacgcctg
    22561 catatagccc atttttttgc cgcagtcgta actgtcgccg gtcatcagca ttgcatcaac
    22621 ggattgtttt ttcgccagct cggcaatagc atcagtcagc tgaatacgtc cccatgcacc
    22681 aggctgagta cgttccagtt ccggccaaat atcggcagaa agcacatagc gacctacggc
    22741 catgatgtct gagtccagcg tctgcggctg atccggtttt tcgataaatt caacaatgcg
    22801 gctgacttta ccctcacggt ccagcggctc tttagtctgg atgacggagt attcagagag
    22861 gtcacccggc atacgttttg ccagcacctg gctgcggccc gtttcgttga aacgtgcaat
    22921 catggcagca aggttgtaac gtagcgggtc ggcgctggca tcgtcgatca caacgtctgg
    22981 cagtaccacg acaaatgggt tgtcaccaat ggcaggtcgc gcacacaaaa tggagtggcc
    23041 taaacctaaa ggttcgccct gacgcacgtt cataatgatc acgcccggcg gacagatgga
    23101 ctgtacttcc gccagcagtt gacgcttcac gcgctgctca aggagtgatt ctaactcata
    23161 agaggtgtcg aagtggtttt cgaccgcgtt cttggacgcg tgagttacca ggaggatttc
    23221 tttgatccct gcagccacaa tctcgtcaac aatgtactga atcattggct tgtcgacgat
    23281 tggtagcatc tctttgggta tcgccttagt ggcaggcaac atatgcatcc cgagacccgc
    23341 tacaggaata actgctttta aattcgtcat tatttcatcc acctgtaaaa tggttgctga
    23401 attatagctt gttcgatttt tttcgccagc atcaattacc ctgaattgat tactgaatta
    23461 cttgtgatgt tacgccgctt cgttgtggat tgcagtagca ttgttcctaa gtatggctcc
    23521 atttttccag gaatggtcgc aaatctactc cctccgttcc ggcaatctaa agttaatctt
    23581 ttccacatta acaatatggt gattaatcct gtcgatatcg acggagcttt gccctctttc
    23641 attcaccgca tgaacatttg caagagacag cagtgtttct tttttcgcca taaagacgcc
    23701 gcgaacgtct ttgcgcatgt cgaagttcat gctcaatgct ggtccaactg aggattcctg
    23761 catcacattg atatttcgca taaaaagatg ttgcggcttg ttgtgtaact ccagcgaggc
    23821 acgcttcatc tcaatgttag ttagcgacac aaaggagacg gcattcccgg cggagatttg
    23881 gatgccgcgc aatttataag caagatgggt attatccagt tgaatattat tcactcggaa
    23941 attttgcggt atcgagagat atttgccttt aattacccca tagccgatta acatcccggc
    24001 gctattaatc atttcaatat tatcaatcac gaaattgtca caaccgtaaa tagcgactgt
    24061 cgcgttatca atgcccgctt tcttactgaa atccggcgtg atattgcggg cattgatatt
    24121 acgaataaca aaatgtttac cattttcaac atggatcaac tgccgacaat ccgatcccgt
    24181 gatattcgcc acgacaaagt ttttcactgc ctggtcttcc gggtagttgt tatcataagt
    24241 gcttcccgca aggcctatgc cgatgcccca gttgattttg ccgttggtac agttgatacg
    24301 ctcgatgaca tggtcagata tcaaaatatc actgtcgtta attgccacgt tccattcaat
    24361 ggcgtcgccc tgtaagtcgc tgaacttaca gttggtaatg ttggcaccga taatctggtt
    24421 atgaaatccc tggcgtaaga tggcgtaatt agcgtggctg acagtgaggt tatcgatggt
    24481 caggttgcgc atgacccgtt tgtttttgcc gccaatataa atctgcatta ccgggccaaa
    24541 gccgctcatc gccagccctt tgatggtgca gtcagaacca cgcacatcca gggtaatgtt
    24601 atgcataccg ccgccctctc cccctgtcac ctggctgccg tcctgtaaga caaatcgccc
    24661 tctgccgttg ccgcgcaggc ttccaaggat gtgtaacgtt ttgccgggag ggatgaagat
    24721 gccggtgttg atattgtcac aaacgaatcc ggcaggcacg acgaccgttt gcccttcgct
    24781 gaaggcttgt ttaaatgagg cgatccagtc atgtgggttg tagtcgttaa tgttaacgct
    24841 ttgtcgggcg ggaagtgcgc gagcgaaagg ggtatggagg aaggcaagcg ccgagcttgc
    24901 cgtcaggaag gtgcgtcggg agagtgtttt aaatggcata cgttctcctc tataaagcct
    24961 gcagcaagct ggcgagttct cgattaatca cctgctggtt aaaatcgtgt tcgacttttt
    25021 cgcacgcacg tttgacgacc ggagccagtt cgtcggtatc cagttggcta aacgccgcca
    25081 gacgttgtgc cagtgcgcga gcatcgttct caggcagcag ccagccggat ttatcggcct
    25141 ccaccagttc aggaattccg ctatgcagag tagaaaccac cggaataccg accgccatcg
    25201 cttccattag cgccaccgga atgccttcca tatcgccatc cgcgcccgtt accgatggca
    25261 acaggaagac atccgcgtcg tcgagcatct ccttcacttc atggctcggt ttaaagcccg
    25321 gcatctctac cacatcttcc agttgatatt gttcgatgat ggtgcgcagg cgtcgttccc
    25381 acaggccaat gccgaggatg cgatagcgaa atgccacgcc ctgctctttc aactgacggc
    25441 aggcttcaat cgccacatgc aggccttttt tctcggttaa gcgtgcgaca gatatgattt
    25501 ccagcggcgt tgcgggcgct ttcaccggac gcgggctaaa gcgcgtcatg tccacgccca
    25561 tacgcgatac ggcgattttt tctctggggc agcccatttt ttgcagcctt ccggcccaca
    25621 gatcgcttat cggtaacatc aggtcgccac ggcgaaacag ttgttgatat tcgggagtgt
    25681 agtggttgag cacttcccga ctggagatat caataccgtg gaagatggtg gcaattttgc
    25741 cgcgaatgat acccagttcg cgtagttttg ctgcggttac ccccgccggg ccaaaatgag
    25801 cgatgaacac atcggcgcga aacggtgttg cgacctgacc acaaatggca gacaaaatca
    25861 ggttccgcga ctcggcacca tagcgtttga ggttgagcgc atgccaggta tttttacgat
    25921 gaatgccacg taaggtctgg ctggcgcggt ggcgcagttt cgctactttg cccgcaggtt
    25981 cgtcctgtaa ccagcgggtt ctggcgacaa ggttgtattt cgtccatgcc gcgtgggtgt
    26041 tctctgtatc gcctttttgc aaagcgacaa tctccacttc aaagcccata tcaataaacg
    26101 cggtaatttg attgaggacg aaggtttccg acgacagcgg gaatttcaat aagaagaagc
    26161 cgaccttcat ttcacctccc cgatgcgctc aagcacagac tgcaccatct gcattcccgt
    26221 ctgacgttcg cgactgacgg cttcgttaaa tcgagcgttc agcaccggaa gctggcctaa
    26281 ggtatccgca accatcgctt gcaggctgcc gtccagtaaa tgacgaatat caatcgccat
    26341 ctctggcagt cccagctgct gcataatccc ggcggattta tgttcatagt tgatggcaat
    26401 cgccggagta gcaaagttca ttgagataat ggcggagtgc aggcgcgtac cgacggtgag
    26461 ttcacaggct ccgagaattt tgcccatttc cagatcgttg agttcatcca tcactacgtg
    26521 gtaacgggca ggatcgctga tgtgctggcg cagattgagc gccaccatgc ggtcgtcttt
    26581 gttatagctg tcgatacccg tacaggtgga gagcgcaatc acctgatagc cttcatcgag
    26641 aatgcgattg accaccccgg caaaggcttt ttcatacgct tgttgagtgg tgccgagacg
    26701 tttgtcgaac ggtgccagtt cgcgcagggt aattgccact gttttctgtt gtgcggcaac
    26761 gtccagccag tgttgaacgg catagctggc ggtgaagtct tcggtgtggt gatcgaccag
    26821 ccacgcggta tcgacgccat gttccacttt tgcggtggtg atatttctgc gtttcatcaa
    26881 atcaaggctg accgattcgc gcaggatcag cgcgtcgcag tgaccaaaga catagttcgc
    26941 cagttggtta aattgctcat cctggaaggg gccgacgctg tgaccaatca taaacagcgg
    27001 ctttttcgcc ataaatgtgc aaagcgcatg ttcaaactgc ggcacgccgt agagatcgac
    27061 aaaaaacgat ccgccgacct gaataatggc gtcgtaacct gacagcaggc gcacgaagtc
    27121 ggtgaatccc tgggcgatgg cgatattgcg cagcttgcca gtgtcggtga cgcgtgagag
    27181 caatacctga tgctggtagc ggcgacggag gactttttta atgcgcccga caacgcccgc
    27241 cgcgctattg tgttgtgtca tctgcaggaa cagcggatcg cccattaccg ggcggttgag
    27301 cagccaggaa gaactgaccg gatagcggct catcacgtcc acttcggtat gtggattgag
    27361 gatgttgatg gcatcaagta agccgcgcaa gatggcgctg tcgccacgat tgccgcaagt
    27421 gtggttgccc agaataagta atttcatttt ttcctcataa atttgatgcc aggtgaggct
    27481 gtgtttgttg tgttcgttgc cgttttttgc ctgatgcgac gctggcgcgt cttatcaggc
    27541 ctacaggtcc cgagcacaga accgtaggac ggataaggcg ttttacgccg catccggcaa
    27601 ccgttgtcgg aaccgaaaac agcaattcac cccgcccgta aaagcatttt cattttttcg
    27661 ctgcgacaaa actgacgctt cacttccacc accagcggat ggcgtgacag cacaatcatc
    27721 accacaaacg ccagcacgcc cgcggctatt tgcaccgcca gcagcatccc cagcgccagt
    27781 tgccctttca gcacaatgcc cagcgcataa ctgaccacca gcgtcggcaa cgagagataa
    27841 aacggcagcc acaaactcag gatgtactgg cgataactgg aaccaagaac cggtttaatc
    27901 atcacgaagt aactcagaat ggtgttgata atctgcacca gcaggaaacc gagcgtgacg
    27961 ccgattgcgc ccgccatctg cccaccaata acaatcgccg gaataaacag aaatgttttg
    28021 aatacgttga atttaaagct gatatcgacc cgcgctttcg ccatcagcag cgaaccaatc
    28081 gggttaccca cggagcgcag cagacccaca atacacagca attgcagcac cggaataatg
    28141 ctgttccact tttcgccgaa taccagcggt acgaagttat tcgacaccac catcagcccc
    28201 agcagcgccg gaaagttgat aatccccact atcgacagca gcttgtagaa gttaacacgc
    28261 agcttttcgg tgtcgtcctg aattttggca aacgccggaa acaacacgcg ggtgatgatt
    28321 gggttcagct tcatcggcgg tacgacggcc acgttgtacg ccaggttgta tccccctgcc
    28381 acgcccgcgc caagaatacg cgccagcacc agcgttgaaa ggttggtgtt gagatagttg
    28441 atgatgctgt ccgccgtcag ccaggcacca aaacgtaagt tcggtgccac agacgccagc
    28501 gagaagtgca gaccggggca ataaattttg cgtccaaagt agccaaacag cagtgttctc
    28561 accgcactat tgaccagata accgaggatc gcggtcatcg ccagcggcca gaaatgggcg
    28621 ctaactaccg taaaagtgaa gcccgccagc accgcgctgg tttcgatcat gccgattttg
    28681 ttgaactcca gctccttctg catcaacgcg cggaactgtt gcccgtgggg gatcaccaca
    28741 aacgccagcg acaatgtttt aatcaacggt gccaggtccg ggttattcag cacgtcgccg
    28801 atgacatcac tcaacaaaaa caccgccaca cataccacaa gccccagccc gacgttcagc
    28861 cagtacagcg tggtgagttc aaggtgactg atttcttttc gctgaataat cgagttagcg
    28921 ataccgaagt cagaaagcgt atctgccagc gcgataatca ccagcgacac agtcagcagg
    28981 ccgaactggt ggttgtcgat aatccgcgcc agcacggtca tctgcaccag cccgaggccg
    29041 atgatgatca ccgtggcaat agccgaccac ttcgcgccgc tgatggtttt ttcacgtaag
    29101 ctcatatcaa tatgccgctt tgttaacgaa gcccttgaat accgtcagga aaacgatttt
    29161 gatatcgaac cagacgctcc attcgcggat gtactcaagg tcgaactcga cgcgtttttc
    29221 cattttctcc agcgtgtcgg tttcgccgcg ccagccatta atctgcgccc agccggtaat
    29281 gcccggtttc actttatggc gcagcatgta gccttcaatg agctggcgat actgttcgtt
    29341 atgcgctact gcgtgcggac gtggaccgac aatcgacatc cccccggtca gcacattgat
    29401 aaactgcggc aattcgtcca gcgaggtgcg gcgcagaaag ttccccactt tagtgacgcg
    29461 cggatcgttc tgcgtcgcct gggtcaccac tttgtcgttc tccatcactt tcatggaacg
    29521 gaacttccac actttgatcg gcttgccatc cataccatag cgagtctggc ggaaaataac
    29581 cggccctggt gaactgagtt tcaccgccag cgcaatacag cacagcaccg gggagatcag
    29641 cagcagaata agcgtcgcca gcacaatgtc ttccgcacgt tttagcaggc ggttaacccc
    29701 cgaaagcggc gtgtcataca gcggcaccac cggaacgccg ttcatctctt cgagacgtga
    29761 atggagaatg ttgaaggtaa agacgtcggg gatcagcagc accgaacagg tggtatccgc
    29821 cagttgatgg accagttttt tcactcgtgc gccgtcgcac atttgcatcg cgatatagac
    29881 gttatgaatc ttgcccgctt ttgcatcctc gaccagctgt tgcaggttac ccgcccagtc
    29941 gttagaaacg ccgcccggtt tcgggtcgtg gtaaacgccc accacttcaa accctaacca
    30001 cggctgatta cggaaactct ccatcagcat ttgtccggcg gctaaatccc ccgctacggc
    30061 gaccatccgc ttgttgtagc catgattacg cagccagccc gccccaatgc gaatacatga
    30121 acggcaaacc accagtccga tgctggtcag cccataccac gccagccaga ttttcagttg
    30181 cgtgtcgaaa tcattgttga acgccaccag tccggcgctg aaaatcacgc ttaaggtcca
    30241 gttttgtagc aacagggcaa attctgtcgc tgcccgaaca ccgcgccatg agcgataaaa
    30301 atcggtgatg ccgcccagca tctggaacac caccagcgta atcagcgcca ccaacaggtg
    30361 catgtagagg aatgacagtc cgctgacttc gcaaaccagc catagcccgg caaacatgat
    30421 ggtgatatct gaaaagcgtt gcaccataga gattaacgat gcattggttt tcgctcgctc
    30481 gcgctttttt agatttgtca tcgttgttcc tgttattagc cccttacccg cagtggggta
    30541 agggaagatc cgacattact cgttcagcaa cgtcagcaga gttcgcgttc gcgcttccat
    30601 cagcggcaca tcaccgcgcg attccacatt caggcgcacc accggttcgg tattggaggt
    30661 gcgcaggtta aagcgccagt cggcaaaggt catgctgatg ccatcgctgc gatccaccgc
    30721 cagcgcctca cggctaaaat gctgttccac gcggttaatc gcctcaacgg gttgcgccag
    30781 tttgctgttg atctcaccgc ttgccggaaa cgccgccatc cggtcgcgca ccagttcgcc
    30841 cagcgttttt cctttcaggc acaccagttc ggcgaccagc agccacggga tcatgccgct
    30901 gtcgcagtaa gcgaaatcac ggaagtaatg gtgggcgctc atttcgccac cgtagatggc
    30961 gtcttccttg cgcatccgtt ctttaataaa ggcgtgtccg gttttcgaca ttaccggcgt
    31021 gccgcctgca gcagtcacca catcaatagt gttccaagag agacgcggat catggatgat
    31081 cttcgcgccg ggattttttt cgaggaacgc ttctgccagc aggccgacaa tgtagtagcc
    31141 ctcgataaac tgcccttttt cgtcaaacag aaaacagcgg tcaaaatcgc catcaaaggc
    31201 aatgcccata tccgcgccgt gtttgatgac cgcattgcgg gtgtcgtcgc gacattccgg
    31261 cagcagcggg ttaggaatac cgttggggaa attgccgtcc ggtgtgttgt gtactttgat
    31321 taattccacg ggcgcgccga gggctttaaa gcgggcttca atggcgtcca ccaccggacc
    31381 cgctgcgcca ttcccggagt tgatcaccag cttgagcggc gtgagatttt tgacattgat
    31441 ataaccgaac aggtgatcaa cgtaagcgtc acgcaggttg atttgctgat agcgaccgcg
    31501 tttggtttca tcgacgggag gaaagtcgtt ggcttcagcc agacgctgga cgtcgcgcag
    31561 tccggtatca ccgctgatcg tgcgagcccc ctcgcgcacc agcttcatgc cgttgtaatc
    31621 catcggatta tggctggcag taacttcaat gccgccatcc acgccgagat ggaacgtggc
    31681 gaaatagatc tcttcggtgc cggacatacc gatatccagc acatcgacgc ccgcatcctg
    31741 taaacctttt gccagcgcca gttttaaggt ttcgctggtg agacggacat caccgcctaa
    31801 cacaatggtt ttcggtttga gaaattcgcc ataggcgcga ccaatgcgcc aggcgatatc
    31861 ttcattcagt tcttcgccta atttcccgcg aatatcatag gctttaaagc aggttaattt
    31921 tttcatgtcg ttaccctttt tcaggcaata gttggccctg accgaaacgg ccaattttat
    31981 ttttgtcgtt atttacaccg cggtttcgca ttcattgcct gatgcgacgc tgccgcgtct
    32041 tatcaggcct acaaaaccga accgtaggtc ggataaggcg ttcacgccgc atccgacaaa
    32101 cagcgcctga tgcgacgttt acacccgtcc gtagcgatcc gcgaagcgca ccacatcatc
    32161 ctcttcgaga taagagccgg agcgcacttc aattaaatcg agcggaattt tccccgggtt
    32221 ttccaggcag tgcgtcgccc ccagcggaat ataaatggac tcgttttcac caagcagttt
    32281 gatatcacca tcaatggtga cttttgccgt tcccgcgacc actacccagt gttccgcgcg
    32341 gtgatggtgc atctgtaccg acaaaccctc gcccggtttc acggtgatgc gtttcacctg
    32401 gtagcggtcg cccgcgtcga tagagtcata tttgccccac ggacgataca cttcgcgatg
    32461 cacccgatgc tcatggcgac catcggcttt gatctgctcg accacttttt tcacatcctg
    32521 taccgcgtta cggtcggcaa tcagcaccgc atctttggtc tgcaccacta ccagatcttt
    32581 caccccgacg gtggtgacca ggcctgattc ggcatacaca tagctgtttt cagttttgtg
    32641 attaatcaca tcgccgtggc aaacgttgcc ctcggcggtg tgggcgctga tctcccataa
    32701 tgaagaccag gaaccaacat cgctccagcc cgcatccatc ggcaccacaa cagcatctgc
    32761 cgtaggttcc atgaccgcgt aatccaccga ctcttccggg caggcgagaa acgcttcttc
    32821 atccacacga ataaaatcga gatccggatc gacggcgctc atcgcttttt cgcaggcatc
    32881 gaggatatcc gggcgatatt ttttcagttc ttcgagatag cgtccggcgc ggaacaggaa
    32941 catgccgctg ttccagtaat attcgccgct tgctacatag gcctgggcgg tttccagatt
    33001 cggtttttcg acaaactgcg ccacttcaaa ggccaccgta tcctgctcac ccacagacac
    33061 ttcaccgcga cgaatatagc cataaccggt ttctggtaga tccggcacaa tgccaaaggt
    33121 caccagcttg cccgcttcgg cataaggcat ggcattacgc acggcggcac ggaacgcgtc
    33181 ttcatcggca atcacatgat ccgccgccaa taccagcatt aacgggtcgc tctccgggct
    33241 atgacgtttt gccgccagcg ccgccagtgc aatggcaggt gcagtgttac gccctgccgg
    33301 ttcgagaata atgttctcgg tgagtttgtt cagttgacgc agctgttccg cgacaataaa
    33361 gcggtgctgc tcattgcaaa tcaccaccgg gctttcgcac tccacgccgt tcaggcggca
    33421 gatggtggtt tgcagcatgg tgagatcgcc tttcaggcat aaaaactgct tggggtaaag
    33481 tacgcgggaa agcggccata aacggctacc ggagccacct gccatcacaa ctggatagag
    33541 tttcgactgc gccataatta tccccgaata tcatttataa attgacgtaa cacgttctct
    33601 ttatcgagcg tgcgttcggc atattcacgt gccaccgtgt tatgtttggg cagcaggagc
    33661 gcctgacgaa tccccgccac cagcgcctcg accgattccg gttctacgca aaccgcaata
    33721 cccggaaagg tttcgcaaag ctgccccagt tctgtgtggg cctctgcggt aatcaccgcg
    33781 ttaccgccta ccgccagaat attggtcagt ttcgacggca atacggcatc tgccgcgccg
    33841 cgtttttgca ccaccagatg gcaatcgccc atcttcagca gtgcaggtaa agcgtcatac
    33901 gattgcagcg ggaaaaattg catgttgcgc agtccacgct gctgcgccat tttttccagc
    33961 cgcgctttgc cgccgccctg cccgacaatg gcaaaaatca gcggttcatc gcgcaggaga
    34021 tcggcagctt caataacgtt ttccagcccc tgcttttcac caatattgcc ggagtaaaga
    34081 atgatttttt tgttatccgg caggccaagc tggttacgaa gggcatcaac atcggcgtct
    34141 gcaacatgct gaaaacgggc aatttccgac cagttgggga agaagatgac gttatccgcc
    34201 gccacgcctt tttcgatggc tttattcatc atcgaacgcg aaatcgtgga aacgttatcg
    34261 acgttatgca gtccgctacg ttcgaacgcc gttgccagct gtgccacttt gccgcctttg
    34321 ccttttccgg caaggcccag ccccagcatg gcgtccactt cgtaatcctg aatatgcagc
    34381 acggtacgcg caccagagag cttcgccagc aggcgcattc ccggcgtgca aaacagcgtt
    34441 ggcactacgc caataatgcg atccggcttc cagcgacgtt gcgccatcag tggaaagaaa
    34501 ctgctgacgg caaaactgcc gagatgcaac aagcgtttca gggtgctcgg ctgttttggc
    34561 acgtacagcg ggcagcgcca caccgtggcg gccccttctt ctcgtttgta gcgccaggcg
    34621 gaatagttct cacccacctg ccattgcggg tagtaaggcg gtgcggtaat gacccgcacc
    34681 tcatgacctt gtgccgccaa ccattccacc atctcgccgg tgtatttgcc gatgccggtt
    34741 aactccggcg agtagttaat gccgtagacc agtattttca taatccgggt actccggcac
    34801 gcttctcagc gagaaaatag gctcggctgt tagcgtgaac atcgttactg gcgagcagcg
    34861 cgtccggcgt cagccagcgg taatcgtcat gctgctcatc cggcagtaac agctcttctt
    34921 ccgctactct gaagcgaaaa ccgagcacca catagtgagt ggtgaaatcc gtgccggaga
    34981 agttatcgtc ataaaaatgc tgccagacac cgtaaaactg gcctgctgtt atcggcagac
    35041 gcagccccag ttccgccatc gtcagccgct caaatgcggc ttccagcgtt tcgtctttct
    35101 gcacgcgccc tcccggcaca aaccagtaac cctgcgccgg gcggttggtt cttttgccaa
    35161 gcagaaactc gccgcgactg ttctcgacaa taaagtcgag agagacaagc ggtgtggagc
    35221 gcactacagt ggcaaagtct tcctgacgta aaaacatcat tccccccgaa agcggtcttg
    35281 attctcaagg aaccactggt aagtgctggc aagccccgct tccagtgaga tttcgtgata
    35341 ccagccaagc tgatgcaggc gcgtcacatc cagcagtttg cgcggcgtac catccggttt
    35401 gctggcatca aaaaccaccc gacctttgta acccaccact ttggcgatgg tttgcgccag
    35461 ctcgcggata gtgcagtcaa cgcccgtgcc gacgttaatg tgcgacagca tcggttgggt
    35521 attctccagc cagacttcat gcgccagctc catgacatga atgctcgccg ccgccatatc
    35581 atcgacgtgc aggaattcac gcatcggtgt accgctgccc cataccacca cgtccggcgc
    35641 attctgtgcc gtcgcctcgt ggaagcgacg cagcaatgct ggtatcacat gggagttact
    35701 cgggtggaag ttgtcatgcg gcccgtacag gttggtcggc atgacagtgc ggtaatcgcg
    35761 tccatactgg cggttgtaag attcgcacag tttgatcccg gcgattttgg caatggcgta
    35821 aggctcgttg gtcggctcca gcgtgccctg caataactcg ctttctgcca tcggctgttt
    35881 tgccagtttc gggtagatgc aggacgatcc gagaaacatc agtttgttca cgtcgttctg
    35941 atgcgcggcg tgaatgatgt tgctctcaat catcatgttc tggtagatga aatccgccgg
    36001 ataggtgttg ttggcaacaa tgccgcccac tttcgccgcc gccagataga cctggtcaat
    36061 gcgttcgctg gcaaagaaat catgcacggc gcggctgtcc agcaggttca g
//
LOCUS       DAJTYL010000028        35196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYL010000028 DAJTYL010000000
VERSION     DAJTYL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.028"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            143..750
                     /locus_tag="OO572_001368"
                     /pseudo
     CDS             143..750
                     /locus_tag="OO572_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(777..1682)
                     /gene="yijE"
                     /locus_tag="OO572_001369"
     CDS             complement(777..1682)
                     /gene="yijE"
                     /locus_tag="OO572_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6660429.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1776..3956)
                     /gene="katG"
                     /locus_tag="OO572_001370"
     CDS             complement(1776..3956)
                     /gene="katG"
                     /locus_tag="OO572_001370"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6660430.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4285..5175)
                     /gene="metF"
                     /locus_tag="OO572_001371"
     CDS             complement(4285..5175)
                     /gene="metF"
                     /locus_tag="OO572_001371"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6660431.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(5524..7956)
                     /gene="metL"
                     /locus_tag="OO572_001372"
     CDS             complement(5524..7956)
                     /gene="metL"
                     /locus_tag="OO572_001372"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6660432.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7959..9119)
                     /gene="metB"
                     /locus_tag="OO572_001373"
     CDS             complement(7959..9119)
                     /gene="metB"
                     /locus_tag="OO572_001373"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6660433.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9396..9713
                     /gene="metJ"
                     /locus_tag="OO572_001374"
     CDS             9396..9713
                     /gene="metJ"
                     /locus_tag="OO572_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6660434.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9897..10505
                     /locus_tag="OO572_001375"
     CDS             9897..10505
                     /locus_tag="OO572_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6660435.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(10566..10778)
                     /gene="rpmE"
                     /locus_tag="OO572_001376"
     CDS             complement(10566..10778)
                     /gene="rpmE"
                     /locus_tag="OO572_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6660436.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            10981..13179
                     /gene="priA"
                     /locus_tag="OO572_001377"
     CDS             10981..13179
                     /gene="priA"
                     /locus_tag="OO572_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6660437.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            13335..14360
                     /gene="cytR"
                     /locus_tag="OO572_001378"
     CDS             13335..14360
                     /gene="cytR"
                     /locus_tag="OO572_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6660438.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            14452..15411
                     /gene="ftsN"
                     /locus_tag="OO572_001379"
     CDS             14452..15411
                     /gene="ftsN"
                     /locus_tag="OO572_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6660439.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            15504..16034
                     /gene="hslV"
                     /locus_tag="OO572_001380"
     CDS             15504..16034
                     /gene="hslV"
                     /locus_tag="OO572_001380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6660440.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            16044..17375
                     /gene="hslU"
                     /locus_tag="OO572_001381"
     CDS             16044..17375
                     /gene="hslU"
                     /locus_tag="OO572_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6660441.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            17442..18368
                     /gene="menA"
                     /locus_tag="OO572_001382"
     CDS             17442..18368
                     /gene="menA"
                     /locus_tag="OO572_001382"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6660442.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            18461..18946
                     /gene="rraA"
                     /locus_tag="OO572_001383"
     CDS             18461..18946
                     /gene="rraA"
                     /locus_tag="OO572_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6660443.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(19031..19276)
                     /gene="zapB"
                     /locus_tag="OO572_001384"
     CDS             complement(19031..19276)
                     /gene="zapB"
                     /locus_tag="OO572_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6660444.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            19702..20547
                     /gene="glpF"
                     /locus_tag="OO572_001385"
                     /pseudo
     CDS             19702..20547
                     /gene="glpF"
                     /locus_tag="OO572_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            20587..22077
                     /gene="glpK"
                     /locus_tag="OO572_001386"
     CDS             20587..22077
                     /gene="glpK"
                     /locus_tag="OO572_001386"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6660445.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            22307..23317
                     /gene="glpX"
                     /locus_tag="OO572_001387"
     CDS             22307..23317
                     /gene="glpX"
                     /locus_tag="OO572_001387"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6660446.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            23414..24160
                     /gene="fpr"
                     /locus_tag="OO572_001388"
     CDS             23414..24160
                     /gene="fpr"
                     /locus_tag="OO572_001388"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6660447.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(24165..24593)
                     /gene="uspD"
                     /locus_tag="OO572_001389"
     CDS             complement(24165..24593)
                     /gene="uspD"
                     /locus_tag="OO572_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6660448.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(24620..24919)
                     /locus_tag="OO572_001390"
     CDS             complement(24620..24919)
                     /locus_tag="OO572_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6660449.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(25131..25571)
                     /locus_tag="OO572_001391"
                     /pseudo
     CDS             complement(25131..25571)
                     /locus_tag="OO572_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            25672..26271
                     /locus_tag="OO572_001392"
     CDS             25672..26271
                     /locus_tag="OO572_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6660450.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            26379..27146
                     /gene="tpiA"
                     /locus_tag="OO572_001393"
     CDS             26379..27146
                     /gene="tpiA"
                     /locus_tag="OO572_001393"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6660451.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(27201..27956)
                     /gene="cdh"
                     /locus_tag="OO572_001394"
     CDS             complement(27201..27956)
                     /gene="cdh"
                     /locus_tag="OO572_001394"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6660452.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(28063..29052)
                     /gene="sbp"
                     /locus_tag="OO572_001395"
     CDS             complement(28063..29052)
                     /gene="sbp"
                     /locus_tag="OO572_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6660453.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(29372..30334)
                     /gene="pfkA"
                     /locus_tag="OO572_001396"
     CDS             complement(29372..30334)
                     /gene="pfkA"
                     /locus_tag="OO572_001396"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6660454.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(30515..31417)
                     /gene="fieF"
                     /locus_tag="OO572_001397"
     CDS             complement(30515..31417)
                     /gene="fieF"
                     /locus_tag="OO572_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6660455.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(31565..32065)
                     /gene="cpxP"
                     /locus_tag="OO572_001398"
     CDS             complement(31565..32065)
                     /gene="cpxP"
                     /locus_tag="OO572_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6660456.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            32215..32913
                     /gene="cpxR"
                     /locus_tag="OO572_001399"
     CDS             32215..32913
                     /gene="cpxR"
                     /locus_tag="OO572_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6660457.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            32910..34283
                     /gene="cpxA"
                     /locus_tag="OO572_001400"
     CDS             32910..34283
                     /gene="cpxA"
                     /locus_tag="OO572_001400"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6660458.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(34389..35063)
                     /gene="yiiM"
                     /locus_tag="OO572_001401"
     CDS             complement(34389..35063)
                     /gene="yiiM"
                     /locus_tag="OO572_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6660459.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT         8637 a         9372 c         9037 g         8150 t
ORIGIN      
        1 ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg agttttttat
       61 ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga atctgtgact
      121 cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc tgctaacggc
      181 atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac aaatccaggc
      241 caataccaac cttgccatcg cagacggtgc cagacagcaa attggcagca cgctatttta
      301 cgatcgcagc ttacctatcc tggcggtgat gttccgcaag aacgcggtgt atgttccgat
      361 atggtgatcc gcgcattgcg cagccagaag gtcgatttgc agaaactggt tcacgaagat
      421 atggcgaaga attttgctga gtacccgcaa aaatggaagt taaagcgccc ggacagcaac
      481 atcgatcacc gtcgggtgcc taatctggaa acctggttta gccgccacaa caaaacacgc
      541 cccatcagta aaaatgccgg tgactatcaa gcgggcgata ttgtctcctg gcgattagat
      601 aacgggctgg cgcatattgg ggtggcgtca gatggcttcg cccgcgacgg tacgccgctg
      661 gtgatacata acattggcgc aggcgcgcag gaagaagatg tgctgtttag ctggcggatg
      721 gtggggcatt atcgttattt tgttaaataa gagatcgccc cacatgaaga aaaaattcag
      781 atgcttttta cactgacagc ttcttttttc ttacggctca ccagcgccag tgccagcaca
      841 atcagcacaa taccgctacc ttcaacggcc cccggattct caccgagcag ccaccaggaa
      901 aagagtacgc cacaaacggg aacggccagt gtgcttaagc tggcaatact ggcaggcaag
      961 tttttcagca caaacagcca taagctccac gctagcgccg tcgccagaat cgcactgtag
     1021 gccagcgccc agaacacggt gggctgccag tcaatttcac gttgcggcac cagtaaagcg
     1081 accacactca tcaccagcgc cgcatacagc atctgccagg atgttaacga taataaatcc
     1141 acgcgcggat gacgggcgta taggcgttta gcaacaatcg cgctcgcccc ccaactaaca
     1201 ccggagagga ttgccagcat ggcacttttc atcgaagaga aatcgagttg ccacggctgc
     1261 aacaccaaaa ataagccgaa agcggcaatc agaatcgcga aatattgccc acgtcgcagg
     1321 cgttcaccga gaaacaacgc ggcgaaaatc accacccaga acggcatggt atagctcagg
     1381 atcgccacct tccccgcacc tccgctgacc aacgcccact gcgccagacc aaccatcccg
     1441 caggtttgta acagggcaat ggctaaggtg tatttaaacg gcgtcgggcg cattccgcga
     1501 ccacgtaata aaaggacgat gaataaaacg agagcgccga aaatgcagcg taaggcggta
     1561 aagtcgaagg caccgatgta actggtgact tgcttcatga aaatccagct ataactccag
     1621 ataagtgtga gcacaaccaa gccactgatt gccagtgggt tgctctttcc tgcggcagac
     1681 atgagagatc cagtgtgtag ttttcctgtg gacactatac gccggtaaag aaacttcagc
     1741 gactgcctga aagacagtcg ctgaagaagg tcagattaca gcaggtcgaa acggtcgagg
     1801 ttcatcactt tcacccatgc cgctacgaag tctttaacaa acttctcgtg ggcatcgctg
     1861 ctggcgtaaa cttccgctac tgcacgcagg acagagttag aaccaaatac cagatccgca
     1921 cggctggctg tgtatttcac ttcgccggtt tcgcggtcac gtccttcgaa cagctctttc
     1981 gattcgtcag tcgctttcca ctcgtaacgc atatccagca agttcacgaa gaagtcattg
     2041 ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa gttggcaccc
     2101 agtacacgca tcccgcccac cagcgcagtc atttccggcg cggtcagcgt cagttgctgt
     2161 gctttgtcga tcagcagtga ctcggtggtg gaaacgtcca gacgagcgcg atagttacgg
     2221 aaaccgtcag caattggctc cagcagctca aacatctcaa tgtcagtctg atcctgacgc
     2281 gcatcaacgc gacccggcgc aaacggtaca tgaatgctca aacctgcggc gcttgcggct
     2341 ttctcaacac caaccacacc agccagcacg atgatatccg ccagcgaggc tttaccagac
     2401 tctttctgga ttttctccag aacaggcaga gcacgaacgg ctgcggcgtt cacatcccag
     2461 tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac caccgcgttt gtcgccacca
     2521 cggaaggtag aagcagatgc ccaggccacc gataccagct cactaacaga cagaccagaa
     2581 tccgcaatcg cgaatttcag atcgataatg tcctgctcgg tcgggttgta gatcggctgc
     2641 ggcagcggat cttgccagat cagatcttct ttcggcactt ccggcccgat gtagcgagat
     2701 ttcggcccca tatccctgtg cgtcagtttg aaccaggcac gggcaaaggc ttcgttgaac
     2761 gcctgcggat cgttgaggaa acgacgagag atcttctcga actcaggatc aaaacgcagc
     2821 gtcaggtcgg tcaccagcat tgtcggttta cgtttcttcg acggatcgaa cggatccggg
     2881 ataatttccg gtgcgtctac cgcttcgaac tggattgctc cagccggact gcgggtctgt
     2941 acccactcat acttgaacag attctcgaag aaatagttgc tccactggat cggcgtctgg
     3001 gtccagacta cttccagacc agaggtaatg gcatctgcgc caacgccgct gccgtaagtg
     3061 ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg acctacgttt
     3121 gatgtcggac cggcaccgtg ggttttaccc agcgtatgac cacccgcaat cagcgccacg
     3181 gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc tgccgcagaa
     3241 agcggttcgc cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc catctcggtt
     3301 gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt gagtcagcca ggctttttca
     3361 tcaccccagt taacatccag atccggttcc cagacgtctt cacgaccggc accaaaaccg
     3421 aaggtacgga agccggagtt ttccagcgcc acgttacctg cgaggataaa caggtcggcc
     3481 caggagattt tctgaccata tttctgtttg attggccaca acaggcgacg cgctttatcg
     3541 aggcttacgt tatccggcca ggagttcagc ggtgcaaaac gttgctgacc acgacccgcg
     3601 ccgccgcgtc catcgattga acggtaagta cctgcgccgt gccaagccat acgaataaac
     3661 agaccggcgt agctgcccca gtcggctggc caccacggct gagattctgt cagcagggct
     3721 ttcagatctt ttttcaggcc gtagtaatct aatttgctga attctttgcg gtagtcaaag
     3781 tcctcaccca gcgggttaga acgattagaa tgttggttta acaggtcaac acggagttga
     3841 tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc tctggtcgtg accgccctga
     3901 tggaacgggc atttgccagt ggctgtggtg ttatggatat cgtctgacgt gctcatcaat
     3961 gtgctcccct ctacagtgtt accgttacga tacacagcgt tagagagaag ttataattga
     4021 attaatccac ggatgcgata gttgagatct tacatattgt tggttaaaga gatgtagatc
     4081 aaattgatcg taattaagct tttttgatta tgtccgaatt cggacaaatg ctttataaaa
     4141 aagggtttta gcttcatatc cttcaagtaa tgagggagaa aacaaggctg acaaccttat
     4201 tttcccgccc tcatttcgag gcagcatttt gtgctctgtt taaaatttgt gatcactgtg
     4261 tgattttcac aaaagccaca ctatttataa accaggtcga acacccagcg tatggcaaat
     4321 cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg aaatctttca ctccttcacg
     4381 gcttaaaatc ttcaccatat ccatggcaat attcgcgccc accagtttgc gggtttcggc
     4441 atcgtcatcc agcccgtcaa acatttgcgc catccacgcc ggaatacgca cgttggtcat
     4501 gtcggcaaat ttcttcgcct gtttaaagtt agataccggc aaaatgcccg ggatgatttc
     4561 cacatcaatg cccgccgata cacagcggtc acgaaaacgc aggtagcttt cgacatcgaa
     4621 gaagaactga gtaatcgcgc ggttggctcc ggcatccact ttgcgtttca gattaagcaa
     4681 atccgcctga gcgctttttg cttccgggtg aacttccgga tacgccgcca cggatatatc
     4741 gaaatctgcc acttctttta acagcgtcac caggtcagaa gcatacattt ctggcttacc
     4801 acttcccggc ggcagatcgc cacgcagtgc cacgatatga cgaataccgt tattccagta
     4861 gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca atgcaagtaa gatgcggtgc
     4921 cgcttccaga ccagtgcgat ctttaatgcc tttaataatg ctgtgcgtac ggtcgcgctc
     4981 gccggagttc gcgccatagg tcaccgatac aaacttcggt ttcaggctgc taaggcgatc
     5041 gatggagttc cacagggtct gctccatttc actggtacgc ggcgggaaaa actcgaacga
     5101 aacgttaatc tgcccctgga cttctgccag gctctgattc agggcatccc gctggctggc
     5161 gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag ttatatgttg tgttgtgcac
     5221 atctatccat ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg cgtcaaccga
     5281 aaaatgacca atgatggtga ggaaaaatca gggaagatga aaaaacttca ggatggtaag
     5341 aaaaagaaaa tccgatatgc aaagcaaacc ggactgcaaa tataaactaa ccatgtactt
     5401 attgcttctt gccggattcg tcgtaaacgc ttcatccggc ctaattctat gataattcaa
     5461 tatatttcat tagaaaccat agacccgaaa acatgagtac cgggcattat taaatttctg
     5521 aaattacaac aactgtgcca gccggttgat atccgactga atcgccccgg cggtgacgtc
     5581 gcgcccagcg ccaggcccac ggatcaccag cggattatcg cggtaccagc ggctttcgat
     5641 ggcaaagacg ttatcgcatg gcagcagtga tgccagcgga tagtcttcac gcaccgcctc
     5701 cacgcccaca cgcgctttgc cgttggcatc gaaacgcgcc acgtagcgca gcaccagccc
     5761 catttcgcgg gccgcttcca gccgttgcac catctgctcg ttaagttcat cgccattttc
     5821 aaagaaatgg tcgatgctgc cgccttcgca atgagcaggc accagcgatt ccacacgtac
     5881 ctggtccggt tcaatgttgt aacccgcttc acgcgccaga atcaccagct tgcgcattac
     5941 gtctttgccg gagaggtcgt cacgcggatc aggctcggtt aagccctgct gccacgcctg
     6001 atccaccagc tcggtaaacg gcacgctacc gtcgaattgc aggaacagcc aggagagcgt
     6061 gccggagaag atcccactga tcgacaaaat ggtatcgccg ctgtcgatca gatcgcgcac
     6121 ggtatggttg atcggcaagc ccgcaccaac ggtggcgttg tatagccagt gacgcccggt
     6181 tttttcgaag gcgtcgtgga tctggcgata tttattgctg tcgcttgctc ccgccagttt
     6241 gttggcactg atgacgtgga aaccgtggct ggcgaaatcg agatactgat cagcaagctg
     6301 ctggctggcg gtaacgtcca acaccactaa atcatcatac ggatgggcgc gcatccacag
     6361 gaaaagcgac tcttcatcct gctcgaccgc ttcatcgttg aagaaggcta acgcgcggct
     6421 ggcgtccagc ccgtcataac tcaacagact gcggcggctg tccaccacgc ctgccagcac
     6481 aaattcaaag ccggtacgcg ccgaaagcgt gctctgctcg cgggcgaaca gttccagcca
     6541 gcgagagccg atattgcctt tgccgaacaa taccaggccg atgcgttttt ctgcgcggaa
     6601 gacggactga tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca gtactgccac
     6661 cagactgatg ccgtcatcgg actgccacgt aaactcgacc ggctggcctt tcagttgctg
     6721 ccagaagcgg tggcaatgca gcgggttacg ggtgacaccc gccccgacca tcgccaccag
     6781 cgccagcccc tgacgcaggc gcagttctcc aggtaatccc gcttcgtcga ggattttcag
     6841 cgcactgtcg gccacttctg aggtgtagca aaattgcaac aactggcgat cgttatgcac
     6901 gccaaccgcc agtggacgca cctgggcacg tttcaggatc tggtcgatct ctttatgcgc
     6961 cagtttgaaa tcctgactgg cgggcacctg aaactcaatc aaacagacat catcgtggct
     7021 ggtgacaata cgcgcaccag taccggaggc cagcacgcgt tcaatgcgcg tggaaccttg
     7081 atccggcgtg tagctacagc gcagctgcag gtcgatttcg ctgccagaaa ctggctgtaa
     7141 agtacgggcg tgaagaacgg gagccgccag acgcgccagt tcgctggcct catccagacg
     7201 cagcaacggc agcaggcagg catctttcac tttacgcggg tcggcactgt ataccccggc
     7261 gacgtcgctc cagatggtta cacgagaaac acccgccagc gcaccgattt gtgtcgcgga
     7321 atagtcggaa ccgttacgcc ccagcaacac cgtttcaccg gcgttgttac ggctgataaa
     7381 cccggtcacc accagacgtt tgctcggatg ttgtaccagt agctgttgca gcaacgggta
     7441 agaaagccct tcatcaacct gcggttgcgc agcgcgttca gcgcgtaaaa actcacgggc
     7501 atcaagccag gccgctggca gcccttgttg attaagtacc gcagacatca ggcgcgccga
     7561 ccacacttca ccgtggccca ccacttccgc atacaccgca tcgttaatac cactgtcgag
     7621 caacgccgcc aggcgctcaa gatcgctgac aaaagcgcta atgaggctat cggcttcttc
     7681 agcgggtagc agaccgctaa tcagatcgca ctgatagcga cgcagcgttt gttgaacctg
     7741 atgcgcagag agacgatcgg tctggcttag tttcaaccag ttaatcaact ggttagtggt
     7801 gctaccggcg gcggaaacca ccatcatatc gtcaggctga gagaactccg ccataatgcc
     7861 cgcgacacgc aaataacact tcacatcagc cagactactg ccaccaaatt tatgcagctg
     7921 acgacctttc gcccctgcct gcgcaatcac actcattttt accccttgtt tgcagcccgg
     7981 aagccatttt ccaggtcggc aattaaatct tcgccatctt caataccggt ggagatacgc
     8041 agcagcgtct cggagatccc ggcggcagca cgcgcttctg gtgccatgcc tgcatgtgtc
     8101 atggttgcgg cgtgagagat taaactttcc actcccccta atgattccgc cagcgtaaac
     8161 aacgacagcc cgcccaggaa acgacacagc gtctgctcat cgccatccag ttcaaaactc
     8221 aacattgcgc caaagccttt ttgctgacgc gcggcaattt catgcccctg attttccggc
     8281 agcgacgggt gatacagttt tttcaccaac ggctgggttt gcagatattt cacaatcgcc
     8341 tgcgcgttgc gctgcgccag ctccatacgc ggcaccagcg ttcgcaaccc gcgtagcagc
     8401 agatagctgt caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca ggcaagttca
     8461 gtgacaacgt ccgggtcttt agcaatcacc acgccagcca ccacgtcaga gtgaccgttc
     8521 agatatttcg tacatgaatg caacaccaga tcggcaccca atgccagcgg attttgtaat
     8581 gccgggctta agaaggtgtt atccaccacg ctcaccgccc cgacttccct tgccagatgg
     8641 cagattttcg caatatccac gacgcgtaac aatggattac ttgggctttc taccagtacc
     8701 agtttgggtt tttctgccag cgctgcccgt aatgcctgtt catcgccttg atcaacaaac
     8761 aacacgcgat agcaaccgcg tttcgccaga ctgtcgaaca ggcgatagct accgccgtag
     8821 cagtcgtgcg gcgcaaccag cagatcgcca ggtttcaaaa agacggtcgt taccaggtga
     8881 atcgcggaca tgccggtatt agtaagtact gcgccagcac cgccttccag ttctgccagc
     8941 gcacgctgaa ccacatcgcg cgttgggttg ccgcgacgcg agtaatcatg cgcgcgcggt
     9001 tcattaaatc cggtaaagtt ataggtgctg gaaagatgga tgggagggac aacgcaacca
     9061 tactgttcgt cgtcatttaa cccgctacgc actgcgatgg tggcctgttt acgcgtcatg
     9121 tgatgaagtt tcctgggctt tgtcggtgaa atgtcaggca ccagagtaaa cattgtgtta
     9181 atggacgtca atacatctgg acatctaaac ttctttgcgt atagattgag caaatcccaa
     9241 atagccgtta aaattatatg cattatcacg ccgacaggtg cattacacga tgtcacggta
     9301 acgcctgtac ggtaaactat gcgggtttac ggtcagtacc cacataaatt gtggggtctg
     9361 gtctcaattt attgacgaag aggattaagt atctcatggc tgaatggagc ggcgaatata
     9421 tcagcccata cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt acggtttcca
     9481 ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag gtgaacaacc
     9541 tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc tttaccgggc
     9601 aacctttgcc ggatgatgcc gatctgcgta aagagcgcag cgacgaaatc ccggaagcgg
     9661 caaaagagat catgcgtgag atggggatta acccggagac gtgggaatac taaagcggaa
     9721 aagagcggcg cggagtggaa tcgcctgatg cactacgctt atcaggccta cgtcatattg
     9781 caatttattg aatttgcacg aacttgtagg ccggataagg cgttcacgcc gcatccggca
     9841 taaacaacga gcacgttgtc tgcgacccac cgctttttat acatggacgt ttaactatga
     9901 aaaacaggct gctgatcctc agcctgctgg tttctgtacc tgcctttgcc tggcagccgc
     9961 aaactggcga catcatcttt caaatctctc gctcatcgca aagtaaagcg atccaactgg
    10021 caacccattc cgattacagc cacaccggta tgctggtgat gcgtaacaaa aagccctacg
    10081 tttttgaagc ggtcggcccg gtgaaataca ccccgctcaa gcagtggatt gcccatggtg
    10141 aaaagggcaa ctacgttgtt cgccgcgttg aaggcggact gagtgttgaa caacagcaaa
    10201 aactgacgca aacggcaaaa cgttatctcg gtaaaccgta tgatttcagc ttctcatgga
    10261 gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ttaccagaac gcgctgggaa
    10321 tgcgcgtggg cgaacagcag aagttgaaag agtttgatct cagcaacccg ctggtccagg
    10381 cgaagctcaa agaacgctac ggtaaaaata ttccgcttga ggaaacggta gtttcaccac
    10441 aagccgtttt cgacgcgcca caactcacca cggttgccaa agagtggccg ctgttttcgt
    10501 ggtgatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc
    10561 cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg
    10621 gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg
    10681 ttcaggtcat gaccaacggt ggagcggatt tttattacgt taccgcaaga gcagctagca
    10741 gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc
    10801 gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata
    10861 cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat
    10921 ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct
    10981 atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg
    11041 ccagaaggca tgacggttaa agctgggtgt cgcgtgcgcg tgccgtttgg caaacagcag
    11101 gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct cactgagcta
    11161 aaagcggtag tcgaagtgct ggatggtgag ccggtgttta ctcactccgt ctggcgattg
    11221 ctgctatggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgccttg
    11281 ccgattttac tacgccaggg gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc
    11341 actgaacaag gtcaggcggt ggatctgaac agcctgaaac gctcacccaa gcaacaacag
    11401 gcgctggcag cgttacggca aggcaaaatc tggcgcgacc aggttgccac gctcgaattt
    11461 aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca
    11521 ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt gcgattgaat
    11581 accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg
    11641 ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt actggaaaac
    11701 gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa
    11761 actatcgccc gttttcgtga acggtttaat gcgcccgtgg aagttctgca ttccggtctg
    11821 aacgacagcg agcgtctttc ggcgtggctg aaagcgaaaa atggcgaggc ggcgattgtg
    11881 atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat tgtcattgac
    11941 gaagagcacg acagttccta caagcagcag gaaggctggc gctatcatgc ccgcgacctg
    12001 gcggtgtatc gtgcgcatag cgagcaaatc ccgattattc ttggctccgc aacgcccgcg
    12061 ctggaaacgt tgtgcaacgt ccagcagaaa aaataccgcc tgctgcgcct gacgcgccgg
    12121 gcggggaatg cgcgtccggc aattcaacac gtgctggatt taaaaggtca gaaggtgcag
    12181 gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc caacaatcag
    12241 gttatcctct ttcttaaccg ccgtggcttt gcgcctgcgc tactgtgcca cgactgcggc
    12301 tggattgccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc gcaacagcat
    12361 ttgcgctgtc accactgtga cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt
    12421 tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac gcttgcgccg
    12481 ctgttccctg atgtgcccat ttctcgtatc gaccgcgata ccaccagccg caaaggggcg
    12541 ctggaacagc aactggcgga agtacatcgc ggcggtgcga ggattttgat cggcacacaa
    12601 atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact ggatgtggac
    12661 ggcgcgctgt tttctgccga ttttcgctcg gcagagcgtt tcgctcagct ttacacccag
    12721 gtcgctggtc gtgccgggcg tgcgggtaaa caaggcgaag tggtgctgca aacgcaccat
    12781 ccggaacatc ctctgttgca aacgttgctc tataaaggct acgatgcctt tgccgaacag
    12841 gcgctggctg agcgacggat gatgcaactg ccgccatgga ccagccatgt gattgtgcgt
    12901 gcggaagatc ataacaatca gcatgcgccg ctgttcctgc aacaactgcg taatctgatc
    12961 ctctccagcc cgctggcaga cgacaaactg tgggttctcg gtccggttcc ggcactggca
    13021 cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc ccgcgtgcgc
    13081 ttgcaacaca tcattagtgg tacgctggcg ctcatcaata caataccgga ttcccgcaag
    13141 gtgaaatggg tactggatgt tgatccgatt gagggttaaa ccgcttacga tgcgaggcgg
    13201 atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc
    13261 tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca
    13321 ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg
    13381 ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat cccgataaag
    13441 tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc
    13501 agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg attgtcccgg
    13561 atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc
    13621 acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta
    13681 tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca aggctgccgt
    13741 ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg
    13801 caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca tttgatgcag
    13861 taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt cccgaagaga
    13921 tgccactgtg tcactaccgc ctgcaaggct atgttcaggc gctgcgtcgc tgcggaatta
    13981 tggttgatcc acaatacatc gcccgtggcg acttcacctt cgaagccgga agcaaagcaa
    14041 tgcaacagct gcttgatctt ccacaaccgc ctactgcagt cttctgccat agcgatgtga
    14101 tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg gaagaccttt
    14161 ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca
    14221 tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc
    14281 aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat
    14341 caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc
    14401 cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa
    14461 cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg
    14521 aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt
    14581 cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga agagtccgag
    14641 acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga agaacgctgg
    14701 cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct
    14761 gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgtca gcttcttgaa
    14821 caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaggttcc gtggaatgaa
    14881 cagacgccgg aacagcgtca gcaaacgcta caacgccagc gtcaggcgca gcaattagcg
    14941 gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg
    15001 cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc agcttctact
    15061 cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg
    15121 cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa
    15181 gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg
    15241 gtacgtgctc agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa caatggctgg
    15301 aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg
    15361 ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa
    15421 aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt
    15481 attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg
    15541 tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga
    15601 aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg
    15661 atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag ggccatctgg
    15721 ttaaagccgc cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg
    15781 aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
    15841 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
    15901 cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt
    15961 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
    16021 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
    16081 tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta
    16141 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
    16201 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc
    16261 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt taccgaagtg ggctacgtcg
    16321 gtaaggaagt tgattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
    16381 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
    16441 acgtgctgat cccacctgct aaaaacaact ggggacagac tgaacagcag caggaacctt
    16501 ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa
    16561 tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg
    16621 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag aagcaaaaag
    16681 cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
    16741 tggtgaaccc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag cacgggatcg
    16801 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
    16861 ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcacc gtttccacca
    16921 aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
    16981 cgaaaccgtc tgacctgatc ccggaactgc aaggtcggct gccaatccgc gttgaactgc
    17041 aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg
    17101 tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc gactccggta
    17161 ttaaacgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
    17221 gtcgtttaca cactgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
    17281 taagcggtca aaatatcact attgacgcag attatgtgag caaacatctg gatgcgttgg
    17341 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
    17401 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
    17461 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
    17521 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc
    17581 tgctggcact aattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
    17641 gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa
    17701 aaggggtcat tacccagcaa aagatgaaac gggcgctcat tattaccgtc gtgctcatct
    17761 gtctctccgg gctggcactg gttgcagtgg catgccatac gctggccgat tttgtcggtt
    17821 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
    17881 cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagtg
    17941 tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga
    18001 ccgcatgcgg cctgctggca acggcagtac tgaatattaa taacctgcgt gatatcaata
    18061 gcgaccgcga aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc
    18121 gttatcatgc ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt aatctctttt
    18181 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accattactg gtgaagcaag
    18241 cccgttatgt gatgcgggaa atggacccgg tggcgatgcg accaatgctg gaacgtactg
    18301 tcaagggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
    18361 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
    18421 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
    18481 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
    18541 cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
    18601 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg
    18661 ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
    18721 ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat
    18781 cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca ttggcgaaag
    18841 cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
    18901 caataccggg attattcttt cagaagatcc gctggatatt gaatgataag aaaggcaccg
    18961 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcgaaaga
    19021 tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
    19081 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
    19141 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
    19201 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
    19261 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt
    19321 cccgattacc ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg
    19381 aagccgcgcc atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca
    19441 tccttctcct agtgacataa tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa
    19501 tgatctaaca aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat
    19561 tttatgacga agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata
    19621 agttaacagc atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca
    19681 ttaactcttc aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct
    19741 gaattcctcg gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa
    19801 gtcgctggtg cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca
    19861 atggccatct acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt
    19921 gcattgtggc tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa
    19981 gttgccggcg ctttctgcgc tgcggcttta gtttacgggc tttactacaa tttatttttc
    20041 gacttcgagc agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc
    20101 actttctcta cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg
    20161 gtgattaccg ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta
    20221 ccacgcggcc ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct
    20281 atgggcccat tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt
    20341 gcctagctgg cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc
    20401 ctggtgccgc ttttcagccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa
    20461 ctgattggtc gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact
    20521 ccttcagaac aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa
    20581 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    20641 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt
    20701 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    20761 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    20821 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    20881 cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat
    20941 atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg
    21001 atcctcgacc atgtggaagg ctctcacgag cgtgcacgtc gtggtgaatt gctgtttggt
    21061 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    21121 accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg
    21181 ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    21241 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    21301 ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac
    21361 acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa
    21421 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa
    21481 ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt
    21541 aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat
    21601 gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg
    21661 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    21721 attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg
    21781 cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc
    21841 gatattctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg
    21901 gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct gcaagagaaa
    21961 gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac
    22021 gcaggctgga aaaaagcggt caaacgcgcg atggcgtggg aagaacacga cgagtaatgt
    22081 caggcacctg aatcgattgc ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc
    22141 aggcctacga gccaatgcca ttttgtgtag gcgggataag gcgttcacgc cgcatccggc
    22201 atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa tcccaccctt
    22261 cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact
    22321 tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg
    22381 acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa
    22441 ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat
    22501 gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga
    22561 tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc
    22621 agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt
    22681 cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa
    22741 tgtagcggcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc tggctaaacc
    22801 acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc
    22861 ggacggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct
    22921 gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga
    22981 tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa
    23041 tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa
    23101 agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat
    23161 taccaaaggc gatctgctgg aaggtattag ccgcaaaggc aatatcgcga ctaccgaaac
    23221 gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga
    23281 tcgcaaagac ccggaaatgc aggtgcacat tctctgagtg atttgatcga ttgagccttc
    23341 cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa
    23401 acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac
    23461 cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt
    23521 taccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa
    23581 ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag
    23641 cccacgattg gcggcactga aaccgggcga tgaagtgcag gtggttagcg aagcagctgg
    23701 cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg caaccggtac
    23761 agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa
    23821 tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca
    23881 agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac
    23941 ggcagcgggg tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag
    24001 cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca
    24061 gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg
    24121 tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac
    24181 ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc cggcatcaaa
    24241 cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata
    24301 atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat
    24361 tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga
    24421 atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata
    24481 tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt attcaccagt
    24541 aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac
    24601 tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct
    24661 cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa
    24721 tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca
    24781 gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg
    24841 tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt
    24901 tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt
    24961 aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt
    25021 ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc
    25081 gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt
    25141 gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct
    25201 tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca
    25261 aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg ctcagttacc cgccagcagc
    25321 atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc
    25381 ttaacagtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc cgcggtctga
    25441 atatcgagta gattcttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg
    25501 cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc
    25561 tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag
    25621 ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca
    25681 gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca
    25741 caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat
    25801 ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct gccactgaac
    25861 gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa
    25921 attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc
    25981 attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct
    26041 caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc
    26101 cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca
    26161 gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt
    26221 gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca
    26281 aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc
    26341 catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg
    26401 gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta
    26461 aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg
    26521 atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc
    26581 tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa gacatcggcg
    26641 ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac
    26701 tgattgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg
    26761 gtgaaaccga agctgaaaac gaagcgggaa aaactgaaga agtttgcgca cgtcagatcg
    26821 acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac
    26881 ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca
    26941 aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc
    27001 agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg
    27061 acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg atcgttaaag
    27121 ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt
    27181 actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg gatttcctcg
    27241 gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaacac aaacgaatta
    27301 tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggcact
    27361 tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc
    27421 gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc
    27481 gccagatttt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga
    27541 aaatggtttt gcgtatgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga
    27601 accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag
    27661 ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc
    27721 atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc ggcattgggt
    27781 ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca
    27841 aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg
    27901 gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt
    27961 ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc
    28021 ggtcgcgaga aaccaccggg tttgataatt atcctaggga aatcagcgtt tgctgatctg
    28081 atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc
    28141 ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc
    28201 agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag
    28261 atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac
    28321 caccgacacc gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc
    28381 tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc
    28441 aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac
    28501 gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg
    28561 cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt
    28621 aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg
    28681 attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag
    28741 acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca cgtcataggc
    28801 cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc
    28861 tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt tccagtgggc
    28921 gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag
    28981 aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc
    29041 ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag
    29101 gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg
    29161 gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc
    29221 ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga
    29281 attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca
    29341 ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc
    29401 agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca
    29461 gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag
    29521 cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct
    29581 ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg
    29641 ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt
    29701 tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa
    29761 ctacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg ccacaataac
    29821 ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt
    29881 cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt
    29941 tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac
    30001 gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt
    30061 ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg
    30121 cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct
    30181 gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt acttccagac
    30241 cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac
    30301 cgccgcttgt caacacacct attttcttaa tcatgactac ctctgaactt tggaatgcaa
    30361 aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat
    30421 agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata
    30481 cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct
    30541 ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc ggaaaacgcc
    30601 gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt
    30661 cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat
    30721 cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttctt
    30781 catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc
    30841 tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt
    30901 accaagacaa ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat
    30961 gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac cgctgaaacg
    31021 agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat
    31081 ctgtcatcgg tgttggagat accagatgtt gaatacccgt caaaaacagg aatagtgccg
    31141 aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa
    31201 acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg
    31261 acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat
    31321 accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg
    31381 ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac
    31441 gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa
    31501 aaaaaacccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg
    31561 tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc
    31621 attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt
    31681 gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac
    31741 gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat
    31801 tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt
    31861 cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta
    31921 tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt
    31981 tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca
    32041 tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac
    32101 gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag
    32161 tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat
    32221 aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag
    32281 atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac
    32341 gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca
    32401 ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt
    32461 gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt
    32521 aatgatcgtg agctggtggc acgtattcgc gcaatcctgc gccgttcgca ctggagcgag
    32581 caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat
    32641 ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt
    32701 accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc
    32761 caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat gcacatttcc
    32821 aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt
    32881 ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg
    32941 ccatcttctg gctgacgctg gtgctggtgt tgatgttggt tttgatgtta cccaagctcg
    33001 attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc
    33061 agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc
    33121 tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg
    33181 aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc
    33241 aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc
    33301 cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagta
    33361 gttctcaatc cgatttcatt aacttattgt ttgaccgccc gctattactg ctgattgtca
    33421 ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc
    33481 gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac
    33541 tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg
    33601 cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc
    33661 gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa
    33721 gcaaggaact cgagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc
    33781 tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca
    33841 accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt
    33901 cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgccctgg
    33961 aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag
    34021 tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg
    34081 ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg
    34141 atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc
    34201 atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt
    34261 tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg
    34321 catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac
    34381 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
    34441 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
    34501 tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
    34561 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
    34621 acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata
    34681 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
    34741 tctccccagc ggaaaatatc gcccatatga acattacttt ccgtgagacc gtcggttgag
    34801 agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcacc
    34861 cagtagagat agtgctcacg aggataatga cacagcgctc tgtctggccc gccgtgaacc
    34921 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
    34981 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
    35041 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
    35101 tatttatttt atgtcagccg tgctgttttc gcggtaagta tccaggcagg aactcaatag
    35161 caaaccggac gaacgttagt tcatccggct gtgggg
//
LOCUS       DAJTYL010000029        34995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYL010000029 DAJTYL010000000
VERSION     DAJTYL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.029"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1503
                     /locus_tag="OO572_001402"
     CDS             <1..1503
                     /locus_tag="OO572_001402"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6660460.1"
                     /translation="TVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRTSSSLSFISYD
                     QALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSL
                     TRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQDNE
                     PVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSF
                     VDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPWGGV
                     INTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNG
                     DAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMN
                     LPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLL
                     KKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILMV"
     gene            1662..2221
                     /locus_tag="OO572_001403"
                     /pseudo
     CDS             1662..2221
                     /locus_tag="OO572_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2222..3871
                     /locus_tag="OO572_001404"
     CDS             2222..3871
                     /locus_tag="OO572_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6660461.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3876..4652
                     /locus_tag="OO572_001405"
     CDS             3876..4652
                     /locus_tag="OO572_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6660462.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4927..7776
                     /gene="rcsC"
                     /locus_tag="OO572_001406"
     CDS             4927..7776
                     /gene="rcsC"
                     /locus_tag="OO572_001406"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6660463.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(7976..8626)
                     /gene="rcsB"
                     /locus_tag="OO572_001407"
     CDS             complement(7976..8626)
                     /gene="rcsB"
                     /locus_tag="OO572_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6660464.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8643..11315)
                     /gene="rcsD"
                     /locus_tag="OO572_001408"
     CDS             complement(8643..11315)
                     /gene="rcsD"
                     /locus_tag="OO572_001408"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6660465.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12053..13174
                     /gene="ompC"
                     /locus_tag="OO572_001409"
     CDS             12053..13174
                     /gene="ompC"
                     /locus_tag="OO572_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6660466.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13286..14341
                     /gene="apbE"
                     /locus_tag="OO572_001410"
     CDS             13286..14341
                     /gene="apbE"
                     /locus_tag="OO572_001410"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6660467.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14415..15479
                     /gene="ada"
                     /locus_tag="OO572_001411"
     CDS             14415..15479
                     /gene="ada"
                     /locus_tag="OO572_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6660468.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15479..16129
                     /gene="alkB"
                     /locus_tag="OO572_001412"
     CDS             15479..16129
                     /gene="alkB"
                     /locus_tag="OO572_001412"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6660469.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16205..17848
                     /gene="yojI"
                     /locus_tag="OO572_001413"
     CDS             16205..17848
                     /gene="yojI"
                     /locus_tag="OO572_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6660470.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18066..18425
                     /locus_tag="OO572_001414"
                     /pseudo
     CDS             18066..18425
                     /locus_tag="OO572_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18659..19690
                     /locus_tag="OO572_001415"
     CDS             18659..19690
                     /locus_tag="OO572_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6660471.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(19812..20300)
                     /gene="eco"
                     /locus_tag="OO572_001416"
     CDS             complement(19812..20300)
                     /gene="eco"
                     /locus_tag="OO572_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6660472.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20435..20599)
                     /locus_tag="OO572_001417"
     CDS             complement(20435..20599)
                     /locus_tag="OO572_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660473.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            20708..21202
                     /gene="napF"
                     /locus_tag="OO572_001418"
     CDS             20708..21202
                     /gene="napF"
                     /locus_tag="OO572_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6660474.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21192..21455
                     /gene="napD"
                     /locus_tag="OO572_001419"
     CDS             21192..21455
                     /gene="napD"
                     /locus_tag="OO572_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6660475.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21452..23938
                     /gene="napA"
                     /locus_tag="OO572_001420"
     CDS             21452..23938
                     /gene="napA"
                     /locus_tag="OO572_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6660476.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            23945..24640
                     /gene="napG"
                     /locus_tag="OO572_001421"
     CDS             23945..24640
                     /gene="napG"
                     /locus_tag="OO572_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6660477.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24627..25490
                     /gene="napH"
                     /locus_tag="OO572_001422"
     CDS             24627..25490
                     /gene="napH"
                     /locus_tag="OO572_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6660478.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25487..25936
                     /gene="napB"
                     /locus_tag="OO572_001423"
     CDS             25487..25936
                     /gene="napB"
                     /locus_tag="OO572_001423"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6660479.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            25946..26548
                     /gene="napC"
                     /locus_tag="OO572_001424"
     CDS             25946..26548
                     /gene="napC"
                     /locus_tag="OO572_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6660480.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26567..27184
                     /gene="ccmA"
                     /locus_tag="OO572_001425"
     CDS             26567..27184
                     /gene="ccmA"
                     /locus_tag="OO572_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6660481.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27181..27843
                     /gene="ccmB"
                     /locus_tag="OO572_001426"
     CDS             27181..27843
                     /gene="ccmB"
                     /locus_tag="OO572_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6660482.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27885..28622
                     /gene="ccmC"
                     /locus_tag="OO572_001427"
     CDS             27885..28622
                     /gene="ccmC"
                     /locus_tag="OO572_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6660483.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28619..28828
                     /gene="ccmD"
                     /locus_tag="OO572_001428"
     CDS             28619..28828
                     /gene="ccmD"
                     /locus_tag="OO572_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6660484.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28825..29304
                     /gene="ccmE"
                     /locus_tag="OO572_001429"
     CDS             28825..29304
                     /gene="ccmE"
                     /locus_tag="OO572_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6660485.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29301..31244
                     /gene="ccmF"
                     /locus_tag="OO572_001430"
     CDS             29301..31244
                     /gene="ccmF"
                     /locus_tag="OO572_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6660486.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31241..31798
                     /gene="dsbE"
                     /locus_tag="OO572_001431"
     CDS             31241..31798
                     /gene="dsbE"
                     /locus_tag="OO572_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6660487.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            31795..32847
                     /gene="ccmH"
                     /locus_tag="OO572_001432"
     CDS             31795..32847
                     /gene="ccmH"
                     /locus_tag="OO572_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6660488.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33058..33705)
                     /gene="narP"
                     /locus_tag="OO572_001433"
     CDS             complement(33058..33705)
                     /gene="narP"
                     /locus_tag="OO572_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6660489.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34025..>34995
                     /locus_tag="OO572_001434"
     CDS             34025..>34995
                     /locus_tag="OO572_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6660490.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRS"
BASE COUNT         8124 a         8566 c         9755 g         8550 t
ORIGIN      
        1 acggtaggcg tggtcgatga aatgatctac gcgctgcaac cagaaatcgc gccgaatatc
       61 ggcaaatttt tctatccgct ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt
      121 atcagctatg accaggcgct ctccagcgag ccggttgcgc ctggcgcgac taaccgcagc
      181 gagcggcgag taaaaatgct tgaacgtcca cggcgtgaag aggtggatac cgcggcatgg
      241 atgccgtcac tcacaaccga taaacaaggc aaagcgtatt tcacgttcct gatgcctgat
      301 tcgttaaccc gctggcgtat caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag
      361 gggcgtgctt atctgcgttc ggaaaaaaat ctctacatga agtggagtat gccaacggtg
      421 tatcgcgtgg gcgacaaacc ggcggcagga ctgtttatct tcagtcagca ggataacgaa
      481 ccggtggcgc tggtgactaa atttgcaggc gctgagatgc gccagacgct gacgctgcac
      541 aaaggggcga attatatttc gctgacgcag aatattcagc aatctggctt gttaagtgca
      601 gaactgcaac aaaatgggca agtgcaggac agcattagca caaaactgtc ttttgtggac
      661 aacagctggc ccgttgaaca gcagaaaaat gtcatgctcg gcggtggcga gaacgcgctg
      721 atgttgcccg agcaggcgag taatatccgg ctacaaagta gtgaaacgcc gcaggagatt
      781 ttccgcaaca atcttgatgc gttagtcgat gaaccgtggg gtggggtgat caacaccggt
      841 agccgtctga tcccgctcag tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc
      901 gctaacgaca ttcgtcagat gattcaggat aaccgtctgc ggctgatgca actggcgggg
      961 cccggagcgc gctttacctg gtggggtgaa gatggcaatg gtgacgcctt ccttacggca
     1021 tgggcatggt acgccgactg gcaggccagc caggcgctcg gcgtaacgca acaaccggaa
     1081 tactggcagc atatgctcga cagttatgcc gagcaggcgg ataatatgcc gttattgcat
     1141 cgggcgctgg tgctggcgtg ggcacaggag atgaatctgc cgtgcaaaac gttgttgaaa
     1201 gggggggatg aagctatcgc ccggcgcgga actaaagatg aagatttctc tgaggaagac
     1261 atccgcgata tcaatgacag cctgatcctc gatacaccgg aatctccact ggcagatgcg
     1321 gtggcaaacg tcttaaccat gacgttgctg aaaaaagcgc agttgaagtc cacggtgatg
     1381 ccacaggttc agcaatatgc gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac
     1441 acggttgtgc tgctcaatag cggtggtgac gctacccaga cggccgctat tttaatggtt
     1501 tgaccgctga gcaatccact attgagcgcg cgctggccat gaactggctg gcgaaatata
     1561 tggcgacaat gcctccagtt gttttgcctg cgcctgcggg cgcatgggct aaacataagt
     1621 taactggagg gggcgaagac tggcgttggg ttggttaggg tgtgccggac attctctctt
     1681 ttggtgacga attatcgccg caaatgtgca ggtccgctgg cgtgaaccgg caaaaacggc
     1741 tcaacaaagt aacattccgg tgaccgttga acgccagttg tatcggctta tccccggtga
     1801 agaagagatg agctttactc tgcagccggt gaccagcaat gagattgaca gcgatgcgct
     1861 gtatctcgat gaaattacgc ttaccagcga gcaggatgca gttctgcgct atggtcaggt
     1921 ggaagtaccg ctcccgccgg gagctgacgt tgagcgcaca acatggggca tttcagtcaa
     1981 taaacccaac gccgcgaaac agcaggggca attgctggaa aaagcgcgta atgaaatggg
     2041 cgaactggcc tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt
     2101 gctgcgcttc tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta
     2161 tgcacctgcg cagcaaagtg tcgcggcagg gagtgaatgg accgggatgc aggtgaaata
     2221 agtgaactgg cgcagaatcg tctggctatt ggcgttggtt actctgccaa cgcttgcgga
     2281 ggagacgccg ttacaactgg tgctgcgcgg tgcgcagcac gatcaactgt atcagctgtc
     2341 gtcatcaggc gtgaccaaag tctcagcatt acctgactcg ctcaccacgc cgttagggag
     2401 tttgtggaaa ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta
     2461 ccagtgtcgc ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac
     2521 gcgcgatacc gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat
     2581 tggcgcagat gtgtgggggc aatactggca acagcgtcag gcacctgcgt ggttagcgtc
     2641 tcttacaacg ctgaagccag aaacctcggt aacggttaaa tcgctgcttg attcattagc
     2701 gacgttacct gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc
     2761 gaaaatcggt gtcgcatcca tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt
     2821 tgccgatgat aaacaggaaa tccgtcaggg tggttttgct ggctggttaa ccgatggtac
     2881 accgctgtgg gttactggca gtggcaccag caaaacggta ctaacccgtt atgcgacggt
     2941 gcttaatcgg gttttgccag tgccgacgca ggttgccagt gggcaatgtg tagaggtgga
     3001 gctgtttgcc cgctatccgc tgaagaaaat cacagcggaa aagagtacga cggcagtaaa
     3061 acccagcgta cttaatgggc gctatcgtgt gacgtttact aatggtaacc acattacgtt
     3121 tgtcagccac ggtgagacga ccttactgag cgaaaagggc aaactgaaat tgcagtcgca
     3181 tctcgatcgt gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccacc
     3241 tgaggcagcg aaagccatga ctgtggcgat acgtactttt ttacagcaaa acgccaatcg
     3301 ggaaggtgat tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc
     3361 agcgaccacg ggggcgcgaa cgatgaccgc ctggacgcag gagctcatct atgcaggcga
     3421 tccggttcat tatcatggca gtcgcgctac cgaagggaca ctttcctggc ggcaagccac
     3481 ggcccaggcg gggcaggggg agcgttacga ccagatactc gcgtttgctt atcccgacaa
     3541 cagccttagc cgctggggag ctccacgctc aacctgccag ttattaccca aagcgaaagc
     3601 ctggctggcg aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa
     3661 cgaaccagac gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca
     3721 gcagaaacgg ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca
     3781 tgagtatctg cacttggcct ttgatggtta tcccaccggg cttgatgaga actatatcga
     3841 aacgctgacc cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt
     3901 tactggtggc actttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg
     3961 caccgctctc tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca
     4021 attacccggc gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg
     4081 gcaaaataaa gaattatgct gcggcgggta aagttcagca gggccggctg gtggtcaacg
     4141 gtgccagtat gccgcagaga attgaatccg atggttcatt tgcacgccct tatattttca
     4201 ctgaaggcag caacagcgtg caggtcatca gcccggatgg gcaaagccgg caaaaaatgc
     4261 agttttactc aacgccgggt acaggaacta ttcgtgcacg tttacggctg gttctctcgt
     4321 gggatacgga caataccgat ctcgatctcc atgttgtcac gcccgatggc gaacacgcct
     4381 ggtacggtaa caccgtgctg aaaaacagtg gtgcactgga tatggatgtc acgacggggt
     4441 acggacccga gattttcgcc atgccagcac cagttcacgg gcgctatcag gtgtatatca
     4501 actactatgg tgggcgcagc gaaacggaat tgacgaccgc ccaactgacg ctgatcaccg
     4561 atgaaggatc ggtcaatgag aaacaggaaa catttattgt accgatgcgt aatgctggcg
     4621 agctgacgct ggtgaaaagt tttgactggt gatccatccc ttcacacaac ctggctctct
     4681 cttttttcag ataagactgc tgggcgatct gccgacgcca ggatataacc attaacaagc
     4741 gtcggcagat tggtcatttc ttatttttcg tactgagaac tgtccatcta ctccggcttt
     4801 cctggttaag gcaatggttt ctaagcgata cgcattattg caatggtgac agaacattgc
     4861 ggtaacacgc ttttaccgct accttaacca cactccatcg gtcacctgag gcggagcttc
     4921 tcccctttga aataccttgc ttcttttcgt acaaccctga aagcctcgcg ctacatgttc
     4981 agagcattgg cgttagtgct ctggctgttg attgcttttt catccgtttt ttacatcgtt
     5041 aatgcgttac atcagcgaga atcggaaatt cgtcaggaat ttaatctgag ttccgatcag
     5101 gctcagcgct ttattcaacg cacctctgat gtgatgaaag agcttaagta catcgccgaa
     5161 aatcgcttat cggcagaaaa cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat
     5221 gtgcctgcgt ttgaaccgct gtttgccgac tccgattgtt ccgcaatgag taacacctgg
     5281 cgaggttctc tggagtcatt ggcgtggttt atgcgctact ggcgcgataa tttttctgcg
     5341 gcttacgatc tcaaccgggt atttttaatc ggcagtgata acctctgcat ggccaatttc
     5401 ggtctgcgtg atatgccagt ggaacgcgat accgcgttga aagcgttgca tgaacgcatc
     5461 aataaatatc gaaatgcacc acaagatgat agcggcagta acctctactg gatcagcgaa
     5521 ggtccgcgcc cttgcgtcgg gtatttttac gcgttgacgc cagtttatct ggcgaaccgg
     5581 ttgcaggcgc ttttgggtgt cgagcagacc atccggatgg agaacttttt cttaccgggt
     5641 acgttgccga tgggggttac cattcttgat gaaaatggtc ataccctgat ttcgcttacc
     5701 ggaccagaaa gtaaaattaa gggcgatcct cgctggatgc aggaacgctc ctggtttggc
     5761 tatacggaag ggttccggga gctggtgctg aagaaaaatc tgccaccctc atcgctaagc
     5821 atcgtgtatt cggtgccggt tgataaggtg ctggaacgca ttcgcatgtt gatccttaac
     5881 gcaattttgc tgaatgtgct tgccggagct gcattgttta ctctcgcgcg gatgtacgag
     5941 cgacgtattt tcattccggc ggaaagcgac gccctgcgac tggaagaaca tgagcagttc
     6001 aatcgcaaga ttgtcgcctc cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc
     6061 gtcaatattt taagtaacga actggcgcat acctatctca atatgcttac gcatgaggac
     6121 cgccaacgac tgacgcaaat tatctgtggg cagcaggtca attttgttga tgtcctgacc
     6181 agcaacaata ccaatctgca aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg
     6241 gccatttgtg tgctggtgga tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag
     6301 atggcacaag cagcggaaca ggcgagccag tcaaaatcga tgttccttgc caccgtcagt
     6361 catgagctgc gaacgccgct gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa
     6421 gagttaccga aaggcgtcga tcgtctggtg acggcaatga acaactcttc cagcctgttg
     6481 ttgaaaatta tcagcgatat tctcgatttc tcgaagattg aatcggaaca gttgaagatc
     6541 gaaccgcgtg agttttcacc gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg
     6601 ctggtggtac gcaagcagtt aggcttgtac tgctttattg aaccggatgt gccagtggcc
     6661 ttaaatggcg acccgatgcg tttacagcag gtcatctcca acctgttgag taacgccata
     6721 aaattcaccg ataccggctg tatagttttg catgttcgcg ctgatggcga ttatctctct
     6781 atccgtgttc gcgataccgg cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat
     6841 cccttcttcc aggttggaac gggcgtacag cgaaacttcc aggggaccgg tctgggtctg
     6901 gcgatttgtg aaaaactgat cagcatgatg gacggcgata tctcggtaga ttcagaaccg
     6961 ggaatgggca gccagtttac cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa
     7021 aaaggcgtgg aagggttgag tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc
     7081 tgtcagttcc tggaaaccag tttgcagcgc agcggcatcg tcgttacaac atacgaaggg
     7141 caggaaccga ctcccgaaga tgtgttaatc actgacgagg tagtgagtaa aaaatggcag
     7201 ggcagagcgg tagtgacctt ctgtcgtcgc catattggta ttccgctgga gaaagcgcca
     7261 ggggagtggg tacacagtgt ggctgctccg catgagctac cggcattgtt ggcgcgtatt
     7321 tatttgatcg agatggagag cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg
     7381 gtcagcgata acgacgatat gatgattctg gtcgtggatg atcatccgat taaccgacgt
     7441 ctgctggcag atcagttggg atcgttgggc tatcaatgta aaaccgcgaa tgatggcgtc
     7501 gatgcgctta atgtacttag taagaatcat attgatatcg tgcttagcga cgtcaacatg
     7561 ccaaatatgg atggttaccg cttgacgcaa cgcattcgtc agttgggact gacgttgccg
     7621 gtaatcggag taactgctaa tgcgttggct gaagagaagc agcggtgtct ggagtccggt
     7681 atggacagct gcctctcgaa gccggtaacg ctggatgtga taaaacagac gctgacggta
     7741 tatgccgaga gggtcaggaa atcgcgggat tcgtaggacg gataaggcgt ttacgccgca
     7801 tccggcattc agtgcaaatg ccagataaga cactgacgcg tcttatctgg cctactttaa
     7861 tgtaatctgt tgaaataatg ggaatcgtag gccggataag gcgtttacgc cgcatccggc
     7921 aatcggtgca aatgccagat aagacactaa cgcgtcttat ctggcctaca gatgattagt
     7981 ctttatctgc cggacttaag gtcacagaag agagataatt cagcagggcg atatcgttct
     8041 cgacgcccag cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt
     8101 tcagcttttt agcgatctcg gtcactagga agccttccgc aaacaggcgc agaacttcac
     8161 tctcttttgg cgagagacgc ttgtcaccgt aaccaccagc actgattttt tccaacaggc
     8221 gagaaacgct ttccggggta aatttcttcc ctttctgcag cgcggcgaga gctttcggca
     8281 gatcggtcgg tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac
     8341 taagaatcgc cgggttgttg ttcatagtca gaacaatgat cgacaggctt gggaaatggc
     8401 gcttgatgta cttgattaag gtaatgccat cgccgtactt atcgccaggc atggagagat
     8461 cggtaatcaa cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt
     8521 caaattcgcc gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca
     8581 agactatcgg atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct
     8641 tgctacagca agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga
     8701 acatccttct cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga
     8761 tttagcatgg caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca
     8821 ctggttgctg cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc
     8881 gcataatagc cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc
     8941 acctcttcct gtgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg
     9001 ctcatattga agttgacgca caattgacca ggcccaattt ctcgtacgcc agactcatca
     9061 tcgcttaaaa gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca
     9121 taatcttgac taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac
     9181 aggcgagtga caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca
     9241 tccagtaaac gctcttcttc ctcttcaact tccgggtcag cggcgagcat tttgatatgc
     9301 acagagtagc gtgtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta
     9361 cgtgccagtt gatcgctcag ccagaatgcc agcgggtccg cctttccata acgatcgttt
     9421 tgggtctggt tgataaacgg gaagtgcaaa ttatccattt catgaatact tacgccttct
     9481 cccgtgtcca gaatgcggaa tgtcaggcgg tcttcagagg actcatcctg atcaacctca
     9541 agggtgattt ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata
     9601 cgtcgtaagg catcgcgatc gccgcggcgc atatcgtgtg ctttcagatg attgttaatc
     9661 agcagttgca gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt
     9721 aaatcctgca cggagaacag caccgtctca cttttccagc tatcatccgc aagcatgttc
     9781 gctaactgta tttcttcgac cagccgcacc agcacatctg cctgattcgc cagttgtttg
     9841 ctttccgggg cgttgagttt agccgcactc tccgccaggg actgtgcggg ttctttcagc
     9901 gcatcgccaa tgtttttcat aaaggtcatc cgcccctgct ggtttttctc atacagacgc
     9961 tgcgcctgct tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aataatgaaa
    10021 atttgtgtgc gcggcgcgac ctggctgcgg aacatgcgga tctcatacag ctcgttattg
    10081 atcgtcgcct gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc
    10141 agatgcggca gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga
    10201 tcgtgaacca gcaggccgag cggcaacagt gagactatct cttcattgat tgcccgtaaa
    10261 atgcgcaatt cgttattgac cgccgtgctg gggacgcttt ctgtactgcg gctggagaaa
    10321 tggcggaatg tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt
    10381 ggcagcagaa tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc
    10441 aggcgcatat cggtagagtt gagtgccgag gagatttcaa tcttcgtact gttaaagtgg
    10501 atactgacgc tatccgtccc ttctttctca ttatcattgt ttcccgttgc cgtcgcgtct
    10561 ggctcaaggc ggaaactgtc cagcggcata cccggtggga tcaaatcatt aatcggcaga
    10621 tcaaaagcca cgaccgttgc cagatgtccc ggctggttga acgtggtacg caaggtaaag
    10681 taatgaccgt tctgccaggc taggcggcgc atgttagaaa aactttcgcg ttcatcgagg
    10741 gcgttggcct gttgtaacat ttctgcacga cgtgaatcaa caatgtcact gacggtcgat
    10801 tctttaaatc cggaggtgag atctttaagg ggtagggttg agatcagcac cagactgtta
    10861 tcctgaccat tcaggtaata catcgaccac ggtacatttt ctgcacccca caatgtatcc
    10921 agataagtgg acatccgctg agtcatctca agcgttgagt tgtcgtgaga gccaaagatt
    10981 aacgcttccg ttttgcggcg tggtttctcc agatagtaga catcctgttt caggcgcgtc
    11041 tcttgtaaac cttcgccgga ggagggggag gtcgtcgcgg caatgttgtc gtagatctgc
    11101 ctggtcacgt aacgccaggt atcgacgcgc ttttggattg cgtgggtaat gtcgacaatc
    11161 tggtaacttt tatctttcag ccaagcgtta acggcacttt gtaccattac ccccatcgtc
    11221 accagtaaca caatgatcaa cagtaaaaag aagcgggtaa tgctccccgg taggagtgaa
    11281 aagcgggtcg tggccgttgt ctctttctga cgcattcgtg tttatgacct gttaaaactt
    11341 cgcgaagtga tagggcagta taaagggtac agtgtgaaat tccagactct tacctgattg
    11401 atgattctct attctcccgt gatatttatc atcctgtatg gggggaaata gatgtacaag
    11461 cgccattttg ctgtacaaat aacctacaaa aagcccaacg aaattaattg caaataaaca
    11521 aagattgctg gaaattatgc tgatgttaat tatttgtgaa atagttaaca agcgttatag
    11581 tttttctgtg gtagcacaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaccgaatg
    11641 cgaggcatcc ggttgaaata ggggtaaaca gacattcaga aatgaatgac ggtaataaat
    11701 aaagttaatg atgatagcgg gagttattct agttgcgagt gaaggttttg ttttgacatt
    11761 cagtgctgtc aaatacttaa gaataagtta ttgattttaa ccttgaatta ttattgcttg
    11821 atgttaggtg cttatttcgc cattccgcaa taatcttaaa aagttccctt gtatttacat
    11881 tttgaaacat ctatagcgat aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt
    11941 ggattattct gcatttttgg ggagaatgga cttgccgact gattaatgag ggttaatcag
    12001 tatgcagtgg cataaaaaag caaataaagg catataacag agggttaata acatgaaagt
    12061 taaagtactg tccctcctgg tcccagctct gctggtagca ggcgcagcaa acgctgctga
    12121 agtttacaac aaagacggca acaaattaga tctgtacggt aaagtagacg gcctgcacta
    12181 tttctctgac gacaagtctg tagatggcga ccagacctac atgcgtcttg gcttcaaagg
    12241 tgaaactcag gttactgacc agctgaccgg ttacggccag tgggaatatc agatccaggg
    12301 caacagcgct gaaaacgaaa acaactcctg gacccgtgtg gcattcgcag gtctgaaatt
    12361 ccaggatgta ggttctttcg actacggtcg taactacggc gtagtttacg acgtaacttc
    12421 ctggaccgac gtactgccag aattcggtgg tgacacttac ggttctgaca acttcatgca
    12481 gcagcgtggt aacggcttcg caacctaccg tagcaccgac ttctttggtc tggttgacgg
    12541 tctgaacttc gctgttcagt atcagggcaa aaacggtagc ccggaaggtg aaggcatgac
    12601 caacaacggt cgtgaagctc tgcgtcagaa cggtgacggc gttggcggtt ctattactta
    12661 tgattacgaa ggcttcggta tcggtgctgc agtttccagc tccaaacgta ctgatgacca
    12721 gaacttcggt ctgaaccgtt atgatgagcg ttacatcggt aatggcgacc gcgctgaaac
    12781 ctacaccggt ggtctgaaat acgacgcgaa caacatctac ctagctgctc agtacaccca
    12841 gacctacaac gcaactcgcg taggcaactt gggttgggcg aacaaagcac agaacttcga
    12901 agctgttgct cagtaccagt tcgacttcgg tctgcgtccg tccctggctt acctgcagtc
    12961 taaaggtaaa aacctgggtg tcatcaatgg tcgtaactac gacgacgaag atatcctgaa
    13021 atatgttgat gttggtgcga cctactactt caacaaaaac atgtccacct acgttgacta
    13081 caaaatcaac ctgctggacg acaaccagtt cactcgtgac gctggcatca acactgataa
    13141 catcgtagct ctgggtctgg tttaccagtt ctaatctcga tggatatcga acaaagggcc
    13201 tgcgggccct tttttcattg ttttcagcgt acaaacacag ttttttggtg tactcttgcg
    13261 accgttcgca tgaggataat cacgtatgga aataagcttt acccgcgtgg cactgctggt
    13321 tgccgcgctc ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa cccacgctac
    13381 tgaagttacc gttcttgaag gcaaaaccat gggtaccttc tggcgtgcca gcatcccggg
    13441 cattgatgcc aaacgcagtg ccgaacttaa agaaaagatt cagacccagc tggacgccga
    13501 cgatcagctg ctttcgacct ataaaaaaga ttccgcgctg atgcgcttta acgactcgca
    13561 aagtttgtcg ccgtggccgg taagtgaagc gatggccgat atcgtcacca cctcgctgcg
    13621 cattggggca aggaccgatg gtgcgatgga tattaccgtc gggccgctgg tgaatctgtg
    13681 gggctttggc ccggaacaac agccggttca aattccgagc caggaacaga tcgatgcgat
    13741 gaaagccaaa accggcttac agcacctgac ggtcatcaat cagtcgcatc agcaatatct
    13801 gcaaaaagac ctgccggatt tatatgtcga tctctccacc gtcggtgaag gttatgcggc
    13861 ggatcacctg gcacgcttga tggagcagga agggatttcc cgctatctgg tgtcggtggg
    13921 cggcgcgctg aacagccgtg gtatgaacgg tgaaggccag ccgtggcggg tagcgattca
    13981 aaaaccaacc gataaagaaa acgcggttca ggctgtggtg gatatcaacg gtcatgggat
    14041 tagcacctct ggcagctacc gtaactatta cgaactggac ggcaaacgtc tttcccatgt
    14101 tatcgatccg caaaccgggc gtcccatcga acacaatctg gtatccgtga cggtgattgc
    14161 tccgacggcg ctggaagccg atgcctggga tactggcttg atggtactcg ggccggagaa
    14221 agccaaagag gttgttcgcc gggaagggct ggcggtttat atgatcacca aagaaggcga
    14281 tagctttaaa acctggatgt caccacagtt taaaagcttc cttgtcagcg aaaaaaatta
    14341 aagcgcaaga ttgttggttt ttgcgtgatg gtgaccgggc agactaaagg ctatccttaa
    14401 ccagggagct gattatgaaa aacgccacat gcttaactga cgatcaacgc tggcaatctg
    14461 tcttagcccg cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc accacgggta
    14521 tcttttgccg tccttcttgc cgcgccagac atgccttgcg ggaaaatgtc tccttctacg
    14581 caaatgccag cgaggcactc gccgccggat ttcgcccctg caaacgttgt cagccagaca
    14641 aagccaatcc ccggcaacat cggttggata aaatcaccca cgcgtgtcga ctgctggaac
    14701 aggaaacgcc tgtaacgctg gaagccttag ccgaccaggt ggcgatgagt ccatttcatc
    14761 tacatcggtt gtttaaagct actaccggaa tgacgcctaa agcctggcaa caggcctggc
    14821 gcgctcgccg tttgcgcgaa tcgctggcga aaggggagag cgtgacgacg tctattctta
    14881 acgccggatt ccccgacagc agcagttact accgcaaagc cgatgaaacg ctgggcatga
    14941 cggctaaaca attccgtcac ggtggcgaaa atctggcggt gcgttacgcg ctggctgatt
    15001 gtgagctggg tcgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg atattgctgg
    15061 gcgatgatga cgcgacatta atcagcgagt tgcagcagat gtttcccgct gccgacaacg
    15121 cgcctgccga tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc ctcaatcaac
    15181 gcgatacgcc gctgacgtta ccgctggata ttcgcggcac tgcttttcag caacaagtct
    15241 ggcaggcact gcgcacgata ccttgtggtg aaaccgtcag ttatcagcaa ctggctaacg
    15301 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    15361 ctatcgtaat accctgtcat cgggtggtcc gtggtgatgg cacactttcc ggttaccgct
    15421 ggggcgtgtc gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    15481 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    15541 tttacggcgt tttgctttta atgctgcgga gcaactaatc cgcgatatta atgacgttgc
    15601 cagccagtcg ccgtttcgcc agatggtcgc tccaggcgga tataccatgt cggtggcgat
    15661 gaccaactgt gggcatctgg gctggacgac ccatcggcaa ggttatctct attcgcccat
    15721 tgatccgcaa acaaataaac cgtggcccgc tatgccacaa agctttcatg atttatgtca
    15781 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    15841 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    15901 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    15961 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    16021 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggtttcatc cactcaccac
    16081 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    16141 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    16201 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    16261 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    16321 ccttatcgaa acggcggata ccagtctgct ggtgttaccg gagtttctgg gattattgct
    16381 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    16441 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    16501 tgaacaactc ggtagcgcct cgttgctggc gggattaacc agcgatgtgc gcaatatcac
    16561 cattgctttt gtgcgtctgc cggaactggg gcaggggatc attctcacta tcggttcatc
    16621 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    16681 caccatctgg ggcggttttg tgctggtggc gcgggtgtac aaacatatgg cgaccctacg
    16741 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    16801 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    16861 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    16921 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    16981 ggctgatacc aacgttgctg cgacctattc gttgacgctg ttattcctgc gtacgccgct
    17041 gctttcggcg gttggcgcat tgccgacgct gctgacggcg caggtggcgt ttaacaagct
    17101 gaacaaattc gcgctcgcgc ctttcaaagc tgagtttccg cgcccgcagg cgtttcccaa
    17161 ctggcaaacg ctggagctgc gtaacgtgac gtttgcttat caggataacg cgttttccgt
    17221 tggtccgatt aatctcacca tcaaacgtgg cgagctgctg tttctgattg gcggtaacgg
    17281 tagcggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    17341 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    17401 ttcggcagtg tttaccgatg tctggctgtt tgatcaactg ctggggccgg aggggcaacc
    17461 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    17521 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    17581 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggtgga
    17641 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    17701 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    17761 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    17821 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    17881 tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat
    17941 tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag
    18001 tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
    18061 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
    18121 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
    18181 gcggcattat gagcgccacg ttggggacct attgacgcga gctggagcct gaatggtcga
    18241 tgaccatggt ggagcgcctg gaggggggcg cgcaggagag ttcgaacggc tagaacaacg
    18301 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
    18361 gcatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
    18421 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
    18481 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
    18541 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
    18601 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaattg gcactgatgt
    18661 gaactatggt gagatcaccc gccagttaat tgcttcctta cagaagaaat ctaacttctc
    18721 gctgcaactc agcagcgaag tccgcgccct aaagcgtaat gacgataaca cctggaccgt
    18781 taccgttgcc gatctgaaaa acggcactgc acagaacatc cgcgccaaat ttgtctttat
    18841 cggcgcgggc ggtgcggcgc tgaagctgtt acaggaatcg gggattccgg aagcgaaaga
    18901 ctacgccggt ttcccggtgg gcggacagtt ccttgtttcg gaaaatccag acgtggttaa
    18961 tcgccatctg gcgaaagttt acggtaaagc atccgttggc gcaccaccga tgtcggttcc
    19021 gcatatcgat acccgtgttc tggacggtaa acgcgtagtg ctgtttgggc catttgccac
    19081 cttctcaacc aaattcctca aaaacggttc attgtgggat ctgatgagtt ccaccaccac
    19141 ctctaacgtg atgccgatga tgcacgtcgg gctggataat ttcgatctgg tgaaatatct
    19201 ggtgagtcag gtgatgttga gtgaagagga tcgttttgaa gcgttgaaag agtactatcc
    19261 gcaagcgaaa aaagaggact ggcgtttgtg gcaagcgggg cagcgtgtgc agattatcaa
    19321 gcgtgatgcc gagaaaggtg gcgtactgcg tctgggtacc gaagtggtca gtgaccagca
    19381 agggaccatt gcggcgctgc tgggggcatc gccaggggcg tcaaccgccg cgccgattat
    19441 gttgaatcta ctggaaaaag tatttggcga tcgcgtttcc agcccgcaat ggcaggctac
    19501 gttgaaagcg atcgttccgt cttatggacg caagctgaac ggtgatgtag cggcaacaga
    19561 acgcgagttg cagtacacca gcgaagtgct ggggctgaaa tacgacaaac cgcaagcagc
    19621 agatagtacg ccgaaaccgc agttgaaacc gcaacccgtt caaaaagaag tggcggatat
    19681 tgcgttgtaa tgatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg
    19741 ctcgggcgtt agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac
    19801 ttcacaacag tttagcgaac ttccgcgttg tcaattttct cttccgcctt ccagacgcgg
    19861 tatttcacat ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc
    19921 agcattccag catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg
    19981 caggccatca tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc
    20041 cagccttcca gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact
    20101 tccagcgtct gaccgattaa cagttctact ttcagggtag attcatcttc ttgcggggtt
    20161 aactgaatca cctgacgctt catgcctgtt tcagcttgtg gataaggcgc gattttttcc
    20221 agtggctgga cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact
    20281 tcaggtagaa tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg
    20341 atgtcgctga aggttaacat attcatcaga tttattaatt tgctttatga ataacttaaa
    20401 gaacattcag acaattttac ctgctctttt agtgttaaat tctgatgagg gagcaaacca
    20461 gcagaaatat ctctggtgaa ttaatcgcta tataaatatg tttataacca tttgaaatgt
    20521 gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta
    20581 ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct ataaaaatgg
    20641 cttattaatt atgcggtcta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa
    20701 ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggttgct ggcgcaaagc
    20761 cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac
    20821 ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcacgggtgg
    20881 ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc
    20941 ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat
    21001 cggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga
    21061 gccaatggca attatctttc gcccgacgct ctccgggatc taccaggcgc aacttaatag
    21121 ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc
    21181 ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg
    21241 aacgaatttc agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca
    21301 gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga
    21361 tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc
    21421 accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
    21481 agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg
    21541 cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc gtttctgcgg
    21601 tactggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct gtcagggcga
    21661 cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat
    21721 catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga
    21781 caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga tggaagagaa
    21841 attcaaaacc gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca
    21901 gtggactatc tgggaaggtt atgccgcgtc caagctgttc aaagcgggct tccgttcgaa
    21961 caacatcgac ccgaacgcac gtcactgtat ggcgtcggca gtagttggct ttatgcgtac
    22021 ctttggtatg gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt
    22081 gctgtggggc gctaacatgg cggagatgca cccgatcctc tggtcacgca tcactaacag
    22141 tcgtctctct aaccagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt
    22201 cgagctggcg gataacggca tcatctttac gccgcaatct gacctggtga tcctgaacta
    22261 catcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca gcaagcacgt
    22321 taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc atccgctgga
    22381 aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg aagattacaa
    22441 agccttcgtt gctgagtata cgctggaaaa aaccgctgaa atgaccggcg tgccgaaaga
    22501 ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg
    22561 gacgatgggc ttcaaccagc atacccgtgg tgtgtgggct aacaacctgg tctacaacct
    22621 gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca
    22681 accttctgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga
    22741 catggtggta actaacgaga aacatcgtga tatctgtgag aagaagtgga atatcccgag
    22801 cggcaccatt ccggcgaaaa tcggtcttca tgcggtggca caagaccgtg cgctgaaaga
    22861 cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat
    22921 caatgaagag cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc
    22981 gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga
    23041 gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc agcaggtaca
    23101 ggcgccaggc gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa
    23161 aactgaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac
    23221 gctgtacgaa gttctgtatg ccacccccga agtgagcaaa ttcccggtat ccgaactggc
    23281 ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt
    23341 cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta
    23401 ccacaaagcg cgcggtctgc gctggccggt ggtgaatggt aaagaaacac agtggcgtta
    23461 cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggtaaacc
    23521 ggatggcaaa gcggtgatct tcgcactgcc gttcgaaccg gcggcggaag caccggatga
    23581 agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat
    23641 gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc
    23701 gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg
    23761 tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt
    23821 gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg atgcgaccga
    23881 tccgctctcg aaagagacgg acttcaagaa gtgcgcggtc aaactggaga aggtgtaagc
    23941 cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt
    24001 tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc
    24061 acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag
    24121 tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc
    24181 gacgctggcc tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg
    24241 cgaaatgtgt gaggacattc cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga
    24301 gattgaatcg atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg
    24361 tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga
    24421 ggccatcacc ttggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc
    24481 gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtat gcgtgctgga
    24541 acaaccggca atcaaggtgt taccgctgtc actggcgaaa ggggagttag gtcaccatta
    24601 ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg
    24661 cgctggagaa aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc
    24721 agttcttcgt gctggggatg tttttgagcg gtccatggtt tggtgtgtgg atcttgcacg
    24781 gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc
    24841 tgcaaagtct tgccagtggt catctgcctg ccacggtggc gttgaccggt gcggtgatta
    24901 tcaccgtgct ctacgccctg gcgggaaagc gattattttg cagctgggtc tgcccgctga
    24961 acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga
    25021 cgatccctcg ccacattcgc tacgtgctgt tggtggtcat tctggtggga tcagccttaa
    25081 ctggcacgct catttgggaa tggatcaacc ccgtttctct gatggggcgt agcctggtaa
    25141 tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg
    25201 ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta
    25261 gcaaaggtgt gattaccgtt gcggccagcg atcggcagaa atgtaaccgc tgtatggatt
    25321 gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc
    25381 cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg
    25441 aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga
    25501 cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt
    25561 ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga cgcaggaagg
    25621 ggccattcgg atgccaaaag agcaggatcg gatgccgctg aactatgtga atcagccgcc
    25681 aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaatc gctgcttgca
    25741 gtgccacggt gtcgaaagct atcgcaccac tggcgcgccg cgtatcagtc ctactcactt
    25801 tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca
    25861 atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa
    25921 aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa
    25981 gcgcctgtgg aaatggtggc gtacccccag ccatctggcg ctggggacgc tgctgttgat
    26041 cggttttgtt ggcggcatcg tcttctgggg gggctttaac accgggatgg aaaaagccaa
    26101 taccgaagag ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat
    26161 ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt
    26221 tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg
    26281 taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc
    26341 acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa
    26401 cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc
    26461 ggtgaaagat gggcaaacct gtattgattg ccataaaggg atcgcgcaca agctgcccga
    26521 tatgcgtgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag
    26581 agagttactt tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa
    26641 cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg
    26701 tttgctgacg gggttgtctc gccctgacgc tggcgaggtt ctctggcaag ggcagccctt
    26761 gcatcaggta cgcgacagct accatcaaac cctgttatgg ataggccatc agccggggat
    26821 caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgatgccgc
    26881 acaatgtctg gaagccttgg cgcaggccgg gcttgccgga ttcgaagata ttccggtaaa
    26941 tcaactttct gccgggcaac aacgccgcgt cgctttagcg cgtctgtggt tgacccgtgc
    27001 cacattatgg atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct
    27061 gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca
    27121 gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc
    27181 atgatgttct ggcgtatttt ccgtcttgaa ctgcgcgtag cgtttcgcca tagcgccgaa
    27241 atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt
    27301 ccggagccgc aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt
    27361 tcatccttgc tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa
    27421 caattgatgt tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac
    27481 tggatggtaa ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga
    27541 atggatgttt atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt
    27601 ggctttctcg gtgcaccggg cgtggcgctg acagtgggac ttaagcgcgg tggtgtgctg
    27661 ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg
    27721 gacgcggctt ctatgcattt gcccgttgat gggtatctgg caattttagg cgcgttgctg
    27781 gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa
    27841 taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc
    27901 atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg
    27961 caattgccag tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg
    28021 ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct
    28081 ggtcgatggg catttatgca tcaatggcgg tggcggcgtt tattggcctt gtctggcaga
    28141 tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta
    28201 ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg
    28261 atgcacgtct gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt
    28321 ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc aggtatcatg gtgctgattg
    28381 gcgtggtgaa tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg
    28441 gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga
    28501 tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt
    28561 tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat
    28621 gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt
    28681 ctggctggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat
    28741 gcaacatcgc gcaattctgc gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg
    28801 tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc
    28861 tgtgccgtgt tggcagggct agcgctgact atcggtctgg tgctatatgc gctgcgctcg
    28921 aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcag
    28981 atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagcgtgcag
    29041 cgcgatccca attcgctgaa agtgaccttc accatttacg atgctgaagg ctcagtggat
    29101 gtctcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag
    29161 ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa
    29221 aactacacgc cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt
    29281 gtttataagg acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcttggcgt
    29341 tagggattgc gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc
    29401 gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat
    29461 ttctggtgct ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca
    29521 actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacttggggc gcgcatgaag
    29581 gttcgcttct gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta
    29641 gtcagcgtat tccgctggat attgtggccc gtgtactggc gataatgggg atggtcagcg
    29701 tcggcttttt gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ctgccgaact
    29761 tcccgattga agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc
    29821 cgcctctgct ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgctt
    29881 ctttgctgag cgggcgtctg gacagcactt atgcgcgttt tactcgcccg tggacgctgg
    29941 cggcgtggat tttcctaacg ctcggcattg tgctcggttc cgcatgggcc tattacgaac
    30001 tcggctgggg tggctggtgg ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc
    30061 tggtggggac tgcactgatg cactcactgg cggtcactga acaacgcgcc agcttcaaag
    30121 cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgttgggg actttcctcg
    30181 tgcgttccgg cgtgctggta tcggtacacg cgtttgcgtc tgatccggcg cgcggtatgt
    30241 ttatcctcgc ctttatggtg ctggtgattg gcggttcgct gctgctgttt gccgcgcgtg
    30301 gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct ttgctgttag
    30361 cgaacaacgt tttgctggtc gccgcgatgc tggtggtgtt gctggggacg ctgctgccgc
    30421 tggtgcacaa gcaacttgga ctgggcagta tttcgattgg cgaaccgttc ttcaacacca
    30481 tgtttacctg gctgatggtg ccgtttgcgc tactgcttgg tgtcggtcct ctggtgcgct
    30541 gggggcgcga tcgcccacgt aaaatccgca atttattgat tatcgccttc atctctacgc
    30601 tggtgctgtc tctgctgttg ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc
    30661 tcggcctggc aatggcctgc tggattgcgg tgctggcaac tgcggaagct gcgctgcgta
    30721 tttcacgcgg cacgaaaacc accttcagtt attgggggat ggtggcggct cacctggggc
    30781 tggcagtgac aattgttggc attgccttta gccagaatta tagcgttgag cgtgatgtgc
    30841 gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc cgtgatgtca
    30901 aagaggtgac tggcccgaac tggcgcggcg gtgtggcgac tattggcgta acgcgagagg
    30961 gtaaaccgga aacggtgctg tatgcggaaa aacgttatta caacactgcc gggtcgatga
    31021 tgaccgaagc ggcaattgac ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag
    31081 agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accatttgtt cgctggattt
    31141 gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat cctcgctatc
    31201 gtaagcgcgt gagtccgcaa aaaactgcgc cggaggccgt atgaagcgca aagtattgtt
    31261 aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa
    31321 tgccgaaggg gatgatccga ccaatctgga atcggcgctc attggcaagc ctgtgccgaa
    31381 gtttcgtctc gaatcactgg acaatccggg gcagttttat caggcggatg tgctgactca
    31441 gggcaaacca gtactgctta acgtctgggc gacttggtgt ccgacctgcc gtgcggaaca
    31501 tcaatatctg aatcagcttt cagcgcaggg cattcgcgtg gtcggcatga actataaaga
    31561 cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg cgctaagcct
    31621 gtttgatggc gacggcatgt tagggctgga tctcggtgtc tatggcgcgc cagaaacctt
    31681 ccttattgac ggcaacggta tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt
    31741 ctgggaagaa gagatcaagc cgctgtggga gaaatacagt aaggaggccg cacaatgagg
    31801 tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac catcgacgtg
    31861 ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga agaactgcgc
    31921 tgcccgaaat gccagaacaa cagcattgcc gattccaact cgatgattgc caccgacctg
    31981 cgtcagaaag tgtatgaact gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat
    32041 atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg
    32101 ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat atacgcccgc
    32161 tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg aacaaagcgt gccggaaggt
    32221 aagcgtgccg gatatgttgt ttatctgccg ggtattgtgg tggcgttaat tgtggctggc
    32281 gtcagctact accagactgg caattatcag caggtgaaaa tctggcagca ggccacggca
    32341 caggctccgg cgttgctgga cagggcgctg gacccgaaag ccgatccgct caacgaagaa
    32401 gagatgtcgc gccttgcgct ggggatgcgt actcaactgc aaaaaaatcc gggagatata
    32461 gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgccag tatcgccacc
    32521 gatgcatacg ctactgcgta tcgcctcgat ccgaagaaca gcgatgccgc actgggatac
    32581 gctgaagcgt tgactcgttc atctgatccc aacgacaacc gcctcggcgg tgaactgcta
    32641 cgtcagctgg tgagaacgga ccatagcaat atccgtgtgc taagcatgta tgcgtttaat
    32701 gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat gttgaaactc
    32761 ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa
    32821 catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca tcgggctaga
    32881 ctgatgacaa agtgcgctta gtttatgccg gatgcggcgt gaacgcctta tccggcctac
    32941 aaaattgtgt aaattcaata aattgatcga tccacgtagg cctgataagc gtagcgcatc
    33001 aggcagtttt gcgtttgtca taaatctcag cccgatgata aatccatcgg gctattttta
    33061 ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc acgcgtgagc ggacattgag
    33121 tttacgcagc agattgcgaa tatgtacttt tactgtctgc tcggaaatat tcaacaccga
    33181 tgcaatctgt ttatttgaca gcccctgtgc cagctcgtgc agaacatcca gctcgcgctc
    33241 cgtcagcacg ctgaagggat cttcttccgc gccaaacatt tcacgttcac gtaagtactg
    33301 attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg gtacgaatcg cttccagcaa
    33361 tacttccggg tcgctgtctt tcaacagata accgtctgcg cctgcgtcta tcagcgcaaa
    33421 gacatcgctg gaggcatcgg atacggtcag gataataatt tgcgcggtaa cgccatccct
    33481 gcgcaaggca ttgagggtat ccaggccact catacctttc atattgagat ccagcaagat
    33541 cacgtcgata tccagtctat tcgccagatc gatcgcgctc gcgccttcgc ccgcttcggc
    33601 gaccacttca aagccaggat caagctccag taactgacga acaccgcgtc gcataagtgg
    33661 atgatcatcc acaatcatca cctgaaaagg cgttgcttca ggcatagtag tctcctgagg
    33721 ttttattaga atcattatgt ttgagcgtag cgttggtaac gctaccaggc gcgtgaatgt
    33781 taccccattt tatgtatgga catgtagtga aaactacaac cttgtcgttc gctttggcat
    33841 catttttatt ataaagacgg aattttgcat cagcttctca tcaagaaaaa caactaaaaa
    33901 atcaatcaca atacttgtgc atttctttaa acagttttta aaaactgact ccgggtatgg
    33961 agctatgggt attttctgta cccaatgctt ttaactgcaa ttaatttcat aggatgaaag
    34021 ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc gcaatatctg ttctggtggc
    34081 tacagcgtta tattcaccca tagcattggc atcaacagtt gagtatggtg agacagttga
    34141 tggtgttatc ctggaaaaag atatccagct ggtttatggg accgccaata atacgaaaat
    34201 caatcctggc ggagaacagc atataaaaga atttggtgta agtagtaata ctgaaattaa
    34261 cggagggtat caatacattg aaatgaatgg taccgcagaa tactcagtat taaatgatgg
    34321 ttatcaaatt gttcaaatgg gtggcgcggc aaaccagact accctcaata atggtgtgct
    34381 acaggtttat ggcccagcga atgataccac gattaaaggc gggcgcttaa tcgttgaaaa
    34441 agatgggggg gccgtttttg ccgctatcga aaagggagga ctactggagg ttaaagaggg
    34501 gggatttgcg cttgcggtag atcagaaaac aggcggtgct attaaaacaa ccacgcgggc
    34561 catggaggta ttcggaacaa accgtctcgg tcagttcgat atcaagaatg gtattgctaa
    34621 taatatgttg ttggaaaacg gcggaagttt gcgagttgaa gaaaatgact tcgcttataa
    34681 tactactgtg gatagtggcg gcttactgga ggttatggat ggcgggactg caactggcgt
    34741 tgataaaaaa gcaggcggga aattaattgt ctcaacgaat gcgctggaag tgagtggtac
    34801 aaacagtaaa ggccaattta gtataaagga tggtgtgtca aaaaattatg aactggatga
    34861 tggttccggg ctcattgtta tggaggacac gcaggccatt gacactatcc ttgatgagca
    34921 tgccactatg caatcgctgg gaaaggatac tggtacgaaa gtgcaggcaa atgcggtata
    34981 tgatctcggt cgatc
//
LOCUS       DAJTYL010000030        34841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYL010000030 DAJTYL010000000
VERSION     DAJTYL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.030"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(18..93)
                     /locus_tag="OO572_001435"
     tRNA            complement(18..93)
                     /locus_tag="OO572_001435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO572_001436"
     tRNA            complement(152..228)
                     /locus_tag="OO572_001436"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="OO572_001437"
     CDS             complement(331..1716)
                     /locus_tag="OO572_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6660491.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO572_001438"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO572_001438"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6660492.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO572_001439"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO572_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6660493.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO572_001440"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO572_001440"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6660494.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO572_001441"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO572_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6660495.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO572_001442"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO572_001442"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6660496.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO572_001443"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO572_001443"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6660497.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO572_001444"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO572_001444"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6660498.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO572_001445"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO572_001445"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6660499.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO572_001446"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO572_001446"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6660500.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO572_001447"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO572_001447"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6660501.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO572_001448"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO572_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6660502.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO572_001449"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO572_001449"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6660503.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO572_001450"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO572_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6660504.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO572_001451"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO572_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6660505.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO572_001452"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO572_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6660506.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="OO572_001453"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="OO572_001453"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6660507.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="OO572_001454"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="OO572_001454"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6660508.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO572_001455"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO572_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6660509.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="OO572_001456"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="OO572_001456"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6660510.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO572_001457"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO572_001457"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6660511.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="OO572_001458"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="OO572_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6660512.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO572_001459"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO572_001459"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6660513.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO572_001460"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO572_001460"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6660514.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO572_001461"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO572_001461"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6660515.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO572_001462"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO572_001462"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6660516.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="OO572_001463"
     CDS             complement(29521..31167)
                     /locus_tag="OO572_001463"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6660517.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="OO572_001464"
     CDS             complement(31170..31220)
                     /locus_tag="OO572_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660518.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO572_001465"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO572_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6660519.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="OO572_001466"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="OO572_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO572_001467"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="OO572_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6660520.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="OO572_001468"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="OO572_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6660521.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(34652..34727)
                     /locus_tag="OO572_001469"
     tRNA            complement(34652..34727)
                     /locus_tag="OO572_001469"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
     gene            complement(34736..34812)
                     /locus_tag="OO572_001470"
     tRNA            complement(34736..34812)
                     /locus_tag="OO572_001470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34776..34778),aa:Asp,seq:gtc)
BASE COUNT         8499 a         9533 c         8798 g         8011 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgatgtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    34741 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    34801 aactaccgct ccaccgaatt cttttacaac caccggtttt a
//
LOCUS       DAJTYL010000031        34050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYL010000031 DAJTYL010000000
VERSION     DAJTYL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.031"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1944
                     /locus_tag="OO572_001471"
     CDS             <1..1944
                     /locus_tag="OO572_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6660522.1"
                     /translation="NFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAY
                     RNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGN
                     AQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTAD
                     LGRVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYN
                     ALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSIS
                     ETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSH
                     STNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDG
                     QYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVK
                     GFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTT
                     NIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAE
                     DGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQ
                     VQAQLKQPKLRGNVQTAQREVAP"
     gene            1941..3029
                     /locus_tag="OO572_001472"
     CDS             1941..3029
                     /locus_tag="OO572_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6660523.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3117..4415)
                     /gene="exuT"
                     /locus_tag="OO572_001473"
     CDS             complement(3117..4415)
                     /gene="exuT"
                     /locus_tag="OO572_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6660524.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4900..6312
                     /gene="uxaC"
                     /locus_tag="OO572_001474"
     CDS             4900..6312
                     /gene="uxaC"
                     /locus_tag="OO572_001474"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6660525.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6327..7814
                     /gene="uxaA"
                     /locus_tag="OO572_001475"
     CDS             6327..7814
                     /gene="uxaA"
                     /locus_tag="OO572_001475"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6660526.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILSTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7897..8448
                     /locus_tag="OO572_001476"
     CDS             7897..8448
                     /locus_tag="OO572_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6660527.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8453..9697)
                     /gene="sstT"
                     /locus_tag="OO572_001477"
     CDS             complement(8453..9697)
                     /gene="sstT"
                     /locus_tag="OO572_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6660528.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10293..11258)
                     /gene="alx"
                     /locus_tag="OO572_001478"
     CDS             complement(10293..11258)
                     /gene="alx"
                     /locus_tag="OO572_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6660529.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11529..12527)
                     /locus_tag="OO572_001479"
     CDS             complement(11529..12527)
                     /locus_tag="OO572_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6660530.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12606..13298)
                     /locus_tag="OO572_001480"
     CDS             complement(12606..13298)
                     /locus_tag="OO572_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6660531.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13375..13878)
                     /locus_tag="OO572_001481"
     CDS             complement(13375..13878)
                     /locus_tag="OO572_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6660532.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13963..15099
                     /gene="rlmG"
                     /locus_tag="OO572_001482"
     CDS             13963..15099
                     /gene="rlmG"
                     /locus_tag="OO572_001482"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6660533.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15384..15698
                     /gene="higB"
                     /locus_tag="OO572_001483"
     CDS             15384..15698
                     /gene="higB"
                     /locus_tag="OO572_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6660534.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15695..16111
                     /gene="higA"
                     /locus_tag="OO572_001484"
     CDS             15695..16111
                     /gene="higA"
                     /locus_tag="OO572_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6660535.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16156..18174)
                     /gene="fadH"
                     /locus_tag="OO572_001485"
     CDS             complement(16156..18174)
                     /gene="fadH"
                     /locus_tag="OO572_001485"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6660536.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18400..20751)
                     /gene="ygjK"
                     /locus_tag="OO572_001486"
     CDS             complement(18400..20751)
                     /gene="ygjK"
                     /locus_tag="OO572_001486"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6660537.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20768..21187)
                     /locus_tag="OO572_001487"
     CDS             complement(20768..21187)
                     /locus_tag="OO572_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660538.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21165..21518)
                     /locus_tag="OO572_001488"
     CDS             complement(21165..21518)
                     /locus_tag="OO572_001488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660539.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21519..21839)
                     /locus_tag="OO572_001489"
     CDS             complement(21519..21839)
                     /locus_tag="OO572_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660540.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(21973..23406)
                     /locus_tag="OO572_001490"
     CDS             complement(21973..23406)
                     /locus_tag="OO572_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6660541.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23469..23918)
                     /locus_tag="OO572_001491"
     CDS             complement(23469..23918)
                     /locus_tag="OO572_001491"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6660542.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23915..27001)
                     /gene="ebgA"
                     /locus_tag="OO572_001492"
     CDS             complement(23915..27001)
                     /gene="ebgA"
                     /locus_tag="OO572_001492"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6660543.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27184..28167)
                     /gene="ebgR"
                     /locus_tag="OO572_001493"
     CDS             complement(27184..28167)
                     /gene="ebgR"
                     /locus_tag="OO572_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6660544.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28385..28716
                     /locus_tag="OO572_001494"
                     /pseudo
     CDS             28385..28716
                     /locus_tag="OO572_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28758..30137)
                     /gene="ygjG"
                     /locus_tag="OO572_001495"
     CDS             complement(28758..30137)
                     /gene="ygjG"
                     /locus_tag="OO572_001495"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6660545.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30555..32075
                     /gene="aer"
                     /locus_tag="OO572_001496"
                     /pseudo
     CDS             30555..32075
                     /gene="aer"
                     /locus_tag="OO572_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32229..32852)
                     /gene="nfeR"
                     /locus_tag="OO572_001497"
                     /gene_synonym="yqjI"
     CDS             complement(32229..32852)
                     /gene="nfeR"
                     /locus_tag="OO572_001497"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6660546.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33129..33874
                     /gene="nfeF"
                     /locus_tag="OO572_001498"
                     /pseudo
     CDS             33129..33874
                     /gene="nfeF"
                     /locus_tag="OO572_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33928..34003)
                     /locus_tag="OO572_001499"
     tRNA            complement(33928..34003)
                     /locus_tag="OO572_001499"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33968..33970),aa:Ile,seq:cat)
BASE COUNT         8306 a         8787 c         8937 g         8020 t
ORIGIN      
        1 aactttctgc cactcggtgc aatcgatggg atgcgtatcg ggtcgacact gagctattta
       61 aaccaggcgc aaagccagca aggaacccca gtaatggttc tgctttcgcg caattctcgt
      121 gttgacgctt atcgtaatga gcaacttttg ggatcgtttt atctcaatag tggttcgcaa
      181 tttattgata ccagttcctt tccgccaggc agctacagcg tggcattaaa agtctacgaa
      241 aataaccaac tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg tctgactgac
      301 ggaaatgcgc aatggttctt acaggcaggt aaaactacat cacaggtttc tgatgatgaa
      361 agttcagctt atcagctagg agtacgcctg ccattacatc cgcaatatga gctctacgca
      421 gggctggcga atgctgatga tgtgagtgct ttcgagttag gtaataactg gacggcagat
      481 ttaggcaggg tggggaatct tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc
      541 aaaggtgata tgcaacaggc caactggagt aatccgggat ggccgacgtt gggcttttat
      601 cggaccaact ctgacggcga tgcttgtaca accgacagca gagagagcta taacgcctta
      661 agctgttatg aaagtatttc cgcgacagtt tcactgaatt ttgtcggctg gaatatgatg
      721 ctgggttata cctgcacaca aaataacact gatgatagtt tgcgttggga taaacagcag
      781 agctttgaaa ataactatct tcgccagaca acagcgcaaa gtatctctga aactgtacaa
      841 cttagcgctt cccgcgctat tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt
      901 ttccatcgta atgacaacgg tggcgataac gacgacaacg gcttgtactt atcgttttcg
      961 ttatctgaca cgccaacgat ggatagcaat aacaacagcc attcaaccaa tgtgcctacg
     1021 gattatcgtt atagcgaaca ggatggcgat caaacgtcat ggcagttatc gcataccttt
     1081 tataacgatt cattcagcca taaagaactt ggcgtaaccg tcggaggcct gaacaccgat
     1141 accataaaca gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt ctacgctacc
     1201 gtatctgata gttatgaccg taagaatcat gatcatcttt cggcctttac gggcacttat
     1261 agctctacgc tggctgtcag tcgctatggc gttaatttgg gagccagtgg tacagacgat
     1321 ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg aacaggatga agagagtcag
     1381 gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac
     1441 agtgtgttgt tcccttatcc tggatttcag tctggttttg ttgaggttaa cgacagtagc
     1501 cagggcaatc agcaggggac aacaaacatc attaacggtg cagggaatcg tgaattaatg
     1561 ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta caactatatc
     1621 ggtcgtttat tactgccggc agcggtgaaa aaattcccga ttgtggggct gaacagcgcc
     1681 atgttactgg tagctgaaga tggcggattt acacttgaaa ttaacggcag cgaaaaagag
     1741 ctctatctgc tttccgggca gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc
     1801 gccagcattc gttacagcgg agatgttact tgtagtgtgg tgacttattc acaattaccg
     1861 gagtcgattc aggttcaggc acagttgaaa cagcctaaat tacgtggaaa cgttcagacg
     1921 gcgcaaaggg aggttgcacc atgagaaatc gattgattgc ggcgatattg ggtttgtttg
     1981 gcacgctcac tggcgttcag gcagctcctg acgtgaccag tgaaattacg tatgatttgg
     2041 catctggtag agcggattat tacttctgga aggatgaagc gtcggcagga aataatggat
     2101 atatgtggta tgaatgttcg tatcctgacc tccaacaaac ctgtacagct aatggaaata
     2161 tatcgacagt acaaatctat ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac
     2221 tcaaaggatt taaaacagcc attgtaagta gcgatgaagc gccgctagga tgcaaggggg
     2281 gcaaagggct tcagacgaat cttaaggatt ctaatagatc ttcatgtaca gaagatggtc
     2341 aacattatta tatatacgat acaaagtttc ttacgctcta ccttgagcag acagagatga
     2401 agaatttgcc gattggtggc gtctggaagg ggaaagttaa attacattcg aacagcccgg
     2461 cccaggacta tttcgcaaat attaccctga atacgctcga ccccaaccat attgacgtgt
     2521 tcttcccgga gttcgcccac gccacgccaa gggtgcagtt agacttgcat ccaacaggaa
     2581 gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg
     2641 gttttaacgg taatgccatc agttatgaaa tcatgctcaa agatgaaggg cgaccagctg
     2701 cagggcgcag agacggttac ttctctatct atcgtcaggg agggaccacc accgacgagg
     2761 gagaacgcat tgattaccgg gtcaaaatgt acaacccgga aaccggtggg caaattgatg
     2821 tgcgcaataa tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg
     2881 tactgcccgg tattcgctat gccgtgatgt gtgtgccgac acccttgact ctggcagtcg
     2941 ataaattcag cgtgatggat aaacaggccg ggtactacat gggcaaattg tcagtaatct
     3001 ttacgccttc cttgccaacc atcaattaac cctgcaaacc ggacaaacgc ttttttctat
     3061 ataaacgacg aagccgcctg ataaggcggc ttcgtcgtta taacttacgt taaacgttaa
     3121 tgttgcggtg cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg tttgttctgc
     3181 aatacggtcc agataaccag cgcacccagc aggtcaaata ctgccagaac tgcgaacagc
     3241 gggctgaagc cgatggtgtc agccagtgca ccgacaacca gcgcaaacag cgtacttgcc
     3301 agccatgcgg acatcccggt taaaccgttc gccgtcgcca cttcgttacg accgaacacg
     3361 tcagaagaga gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc accgatacac
     3421 agcagcataa ttgcgacata cgggttggtg aacagaccga tcatacccgg gccaatcatc
     3481 agcacagcac ccagcgttac gaccatctta cgggaaacga tcaggttcac accaaaccaa
     3541 cgctggaaca gcggcggcag gtaaccaccg aggatacaac cgaggtcagc aaacagcatc
     3601 ggcatccagg cgaacatcgc gatctctttc aggttaaagc cgtaaacttt aaacatgaac
     3661 agcgggatcc acgcgttaaa agtaccccag gccggttctg caaggaaacg cggcagtgcg
     3721 ataccccaga actgacggtt acgcaggatc tgaccaacgg acattttctt cgccgtgctc
     3781 acctggtgct gggcttcctg accattaata atatagtcgc gttcttcatc ggtcagatgc
     3841 ttctggtcgc gcggatgttt atagaaaatc agccatgcca tcgcccagat aaagctcaat
     3901 gcaccggaga tgataaatgc catctgccag ctgtgcatca cgattgccca taccaccagc
     3961 ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa agtagcctac tgcgatggaa
     4021 cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct
     4081 tccgcggcac cgaccgcacc acgagcaaca gctaggccac cccagctacc tgccagcgcg
     4141 gttgcaccac agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc
     4201 agcacatcca gtacataacc tgctaccggt tgcatgaccg tataagcagc agaataggct
     4261 gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca
     4321 gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg
     4381 atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat
     4441 ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa
     4501 ccgaaaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc taattcggct
     4561 tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt gcattccgtc
     4621 gacttatgat ttgcgacggc agaaagataa cttgtcatac aaccttaaaa ggtgagagcc
     4681 atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc cagtactggc
     4741 gcggcctgca gcgagattta ccactttgag agtaattttt ttaactacgt ttattgatct
     4801 aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc agcatcgcac
     4861 cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt tatgactgaa
     4921 gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc aaaagaccag
     4981 ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt
     5041 aaaaacctgt atgacatctg gttgaagggc gatcactaca aatggcgcgc tatgcgtacc
     5101 aacggtgtgg ctgagcgtct gtgtactggt gatgcgtctg accgtgaaaa atttgacgcc
     5161 tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac gcacctcgaa
     5221 ctgcgtcgtc cgttcggtat cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc
     5281 tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg catcatgcag
     5341 cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct ggagcatcac
     5401 gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg gcgtccggac
     5461 aaagccttca atatcgaaca ggcgaccttt aacgactaca tggcgaagct gggcgaagtt
     5521 tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa acgtctggat
     5581 cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt gatgtttgct
     5641 gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg
     5701 agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg tgccgaatat
     5761 gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa caacctgcgt
     5821 cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg tccgatggcg
     5881 gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt
     5941 ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg taacttccag
     6001 ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa cgatcagaaa
     6061 gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtctgctgag ccgctttgtc
     6121 ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata cttccgccgc
     6181 attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac
     6241 ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta cttcgccatt
     6301 gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg ctggataacg
     6361 tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat aaccagactg
     6421 ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat atcgcaaaag
     6481 gggccaacgt catcaaatat ggcctgccga ttggttatgc attggcggat attgcggcgg
     6541 gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat cagtatcgct
     6601 atcaacttga ttttcaggat ctgcctgcgc aagcggcaga tcctgaagtg cagatctatc
     6661 gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgtca accgtgggct
     6721 gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc aacaacgccg
     6781 aaggtaccga cggcgtgttc ctcttcagcc acacctatgg ctgctcacaa ctgggcgacg
     6841 atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag
     6901 tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg
     6961 gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat gatgagatcg
     7021 aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat aaacgcgagc
     7081 caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac ggtctttctg
     7141 gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct aacggcggta
     7201 ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg atggaccatt
     7261 gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttc aaacagtact
     7321 ttattgccca tgatcagccg atctacgaaa acccatcacc ggggaacaaa gcgggcggta
     7381 tcaccacgct ggaagacaaa tcacttggct gtacccagaa agcgggttcc agtgtcgtgg
     7441 ttgacgtgct gcgttacggc gagcgtctga aaacgccagg gctgaacttg ttaagtgcgc
     7501 cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat atggtgctgt
     7561 tcagtactgg tcgcggcacg ccatacggtg gatttgtgcc gacggtgaaa atcgccacca
     7621 acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt cagctgatcc
     7681 acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt gagtttgcca
     7741 acggtaagca aacctgcaac gagcgtaacg acttccgcga actggcgatc ttcaaaagcg
     7801 gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca taatgtgaag
     7861 cgccgtttgc ttttccatac ctgctaagga tttatcatga ccgcgtattg gctggcccag
     7921 ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg tgacgaacgt
     7981 cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt ttttcttctg
     8041 ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt gattacctta
     8101 cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg aattggcctc
     8161 gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt cagtacctgg
     8221 gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc
     8281 tgggtgattc acaacttctg ggcggggtcg ataggcggca cgatgattga gggtagtttt
     8341 ctgcttatga atggcctgaa tatcattcgt ttctggcgga tgcagaaaag gggaattgat
     8401 ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa ggggttaaac aattaattac
     8461 gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca gtgaacagca
     8521 cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg ataataaagc
     8581 cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta caggccagtg
     8641 ggatcagcag caaagaaccc cccgccacgc cggatgcgcc acaggcacac agagaagcca
     8701 ctacgctcaa cagcagcgct gtgggcagat cgaccggaat acccagcgta ttaaccgcag
     8761 ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg gctcccagcg
     8821 gaatagaaac tgaataggta tcgcgatcca gattcagctt ttcacacagc gccatattca
     8881 ccggaatgtt cgctgcagag ctgcgggtga agaaggcata cacaccgctt tcgcgcaggc
     8941 acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc aatgggttaa
     9001 ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc gcgtagcccc
     9061 acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagcccaaaa ataccgaacg
     9121 gtgcgaagcg aatgaccagt ttcaccataa aggtaacggc attcgacata tcattgacca
     9181 ggtttttggt ggtctcgtta ccgtgacgca gtgcgaagcc gaggccgatc gcccacacca
     9241 gaatcccgat gtagttacct ttcagcaggg cgtcgatggg gttggaaacc atgctcatta
     9301 ccagtccgcg catcacttcg acaatgcctg acggcggcga aatatcaccc gcgctactgg
     9361 acaagtgcag ggtagaaggg aaggcaaagc tgaagactac tgcggccaga gctgcagaga
     9421 aggtgcccag cagatagagg aacaaaatag gacggatatt ggttttctgc ccgtgctggt
     9481 ggttagcaat agatgccatt accagcatca acaccaggat gggggcaaca gctttcagtg
     9541 cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt gagatccatg
     9601 ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg ccatgagcca
     9661 gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt cattgtgttg
     9721 tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa
     9781 ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt ttaaagagag
     9841 gtcatgtttt gcaacttaat ggttgcattt atttaacata aacagaggga aggactggct
     9901 actaaggctg attagataac atatgggcaa aactatttgg aatatgagaa cggttgcgct
     9961 cctttaacgt gttatgccat ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa
    10021 tcgccgccgc cccctgcacc cccaggctct ggcgaacaaa atcgccgctg cgcggttccc
    10081 tcagcctatc ccttgcggct accgggtcgg gcgcaaggta acgtccctgt aagatgcgcc
    10141 ctcgccccgg cctccaggga acgcttcggc gattttgacg ccaccaacac ccgtgcggtc
    10201 tttttgtaaa gagctataag ccgattacat aattaatagc tcacctttgt ctggaatgag
    10261 ttatctctag gaatggctta atatacagcg aatcattctc cccgctgctt atcatgccga
    10321 taattcaccc aggcgttgat aataaacgtc atcaccagaa tgccaaacac cacgcccagc
    10381 gagacggcga ttggaatatg gtagaagtcg acaatcagca tcttgatacc gataaacacc
    10441 agaatcaccg ccaggccata tttgagcatc gagaaacgct ctgctacgcc cgccagcagg
    10501 aaatacatcg cacgcaggcc gaggattgca aacaggtttg aggtcagcac aatgaacggg
    10561 tcagtggtca cggcgaagat agccggaatg ctatccacgg caaaaatcac gtcgctcaat
    10621 tccaccagaa tcagtaccag catcagcggt gtggcgtaca gcaatccatt cttacgcaca
    10681 aagaaatgct cgttgtcgat ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc
    10741 agcggcttgt cgccaatgcc tgattcatct tcatgggcga gcgccatctt cacgccggta
    10801 aacagcagga aggcaccgaa gatatacagg atccagtcga actgcgaaat cagccagctg
    10861 cctgtgaaga tcatgatggt acgcagaaca atcgccccga gcacgccata caccagcacg
    10921 cgacgttgta atgccgccgg aacggagaaa tagctgaaca acatcagcca gacaaagacg
    10981 ttatcgaccg ccagcgattt ctcaatcaga taacccgtga gaaaggccag ggcctgtgga
    11041 tcggcaacgg cgcgaccttc ggtttgcacc agataccacc agaaagcggc gttaaacagt
    11101 aacgacagcg tcacccagac cagcgaccag gccgcagcct gtttcatggt catggcatgt
    11161 gccccacgac gcccctgcaa caacaggtcg atagccagca taatggcgac aacaacagcg
    11221 aatccgcccc atagcaacgg cgtgccgaca gtattcatag aagttcctta cacataaaaa
    11281 aacggccaac gtcggaagac gtcagccgct gctttttatg catagacctc gccttccggc
    11341 aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg
    11401 accggatgtg gtttttcact catcgtaatg acgatcgacc ggcaatgaag ttactcccct
    11461 ttgcaggtaa caaagtatga cagaccattc agtcagtcaa tggtctgtta catacctttt
    11521 acactgtttt actttggcaa gtatagtttt acgctatctg ccggaaaaat caccccagtt
    11581 tggcggcgga tctcggtcag cagtttggct gtgatgcgac tgaccgccag ccctggatga
    11641 tccaccagat gctcatccac cagggtagcg aacagctctg cttcgtagag catggtatta
    11701 atatgctgcg gctgggtgag atcctgcatc tggctgccac gcggcacaaa gcagactttc
    11761 tggcattcag acagtttttc aatcaccagc gatcccgctt cgccctgaat ctcgcttccc
    11821 aggacagaat cactgacctt tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc
    11881 accaccacgc cgtgggcgtc aacgccgctt gccagcaaac tcgccgttgc ctgcacgctt
    11941 ttcggctcac caaataacgc caccgccgac gccaggcagt aaaagccgat atccataatt
    12001 gaaccgttag agaatgccgg attaaaggtg ttgggattct caccgtccag gtaacgttga
    12061 taccgcgagg aatactggca atagttgaaa aagactttac gcagtttgcc tgctttcggc
    12121 agcgcctggc gcaacaaatg aaagttcggc aggcaggcgg ttttaaatgc ctcaaacagc
    12181 accacctgat tttcgcgcgc acaggcaatg gcggcatcca cttccgccag attcgacgcc
    12241 agcggttttt cgcaaatcac atgaatttta tggctaagga aaagttgtgt ctgggaaaag
    12301 tgcagggaat tcgggctggc aatatacacc gcgtcaatgg catcgctttc cgccatcgct
    12361 tccagcgacg taaacagatg ctcgacagaa aaatcattgg cgaagtgctg agcctgctca
    12421 aggctgcggg aatatacggc ggttaacttg tatttaccgc tctcatgggc ggcctcgacg
    12481 aactggcgag tgatccagtt cgtaccaatc acagcgaaac gtatcataac gcgtggatac
    12541 tccataaagg gattctgtcg tcaatgtagc acgccttttt catcttcgac gtgcgcagat
    12601 acgcattatt ctttcgccgg acagccatgc tcactaaacg gcccaatcat gaccgatggc
    12661 ccgagatatt tcggctggcg atggaggatt tttgcgtcaa tcaccgcgct gacacgagcg
    12721 agtttctccc gcagttgagt ataaaaaccg tgcttcatat tgaggtctgg cgcgttaaac
    12781 ccgatagcgt cgatgccgta ctgttttgcc agccagatgg tgcgttggtt atgaaattct
    12841 tgcgagataa tggtgatatg gttttcccca aacacctttt tggcacgcac taccgaatcc
    12901 agcgttgaga atccggcata gtcgcaaaag atcactttcg ccggtacgcc tttagcgatc
    12961 aaagcctgct gcatacctga tgcttcatcg taatttttac gtccgttatc gccactcacc
    13021 agtagccatt tcacttttcc ggcgtggtac aacgctgctg cggtatcaat acgtcgggta
    13081 aagtagcgat tacccggcct cgcccccagc aataaaccca cgttacgcgc cgggacggcg
    13141 ttgacatcgc cccaggtcag ctgcttgctg gcgttgacca taacgcgatc ggcgataaaa
    13201 atggtcgctc ctgccacaag taacagtaag caggcaatct ttagagtccg gcgagaaaaa
    13261 caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg tcgttatcct taacgaaagg
    13321 ggggaggtcg attttaggcg ctaaagaaag aaaagataac gcctgacatc attttcagat
    13381 tttgtcctgc ccaagcgaca actgcgttag ccacagccgg gtgtcgaact caagctggtg
    13441 gtactgcggc tccatatggc agcacaattg atagaacgct ttgttatgct ctttctcctt
    13501 caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac ggttccggcg cgttgcgaaa
    13561 cacggtagcg acgcggatct ctgcttttgc tttcagcttg ctgccctgca cacgagaaac
    13621 agcggtatgt aacccgagcg cgttttttag cacatggatc ttattgtcat acatcacttt
    13681 attgatcggc ggggcattac gcagaaactg atttttcaga tcctgagtat attgccagag
    13741 agctttatcg gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca catcgcccag
    13801 acgctgttcg ttaatcagtg ttcgcacctg cgaaagcagc tgctcgggat aaccctgaag
    13861 ataagtaaga ttgctcataa ccgcccacga aaaagagaaa aagggtatac tcacgcaccc
    13921 ttttcagggg caacgtcgaa attttatcat tcaggagggc cgatgagcca cttagacaac
    13981 ggtttccgtt cactgacact acaacgtttt ccggcgacgg atgacgttaa cccgctacag
    14041 gcgtgggaag cggcagatga atatttgctg caacagttgg acgacacaga aatcctcggc
    14101 ccggtgttga tcctgaatga tgcctttggt gcgttaagct gcgcgctggc ggaacataag
    14161 ccgtacagca ttggcgactc atacatcagt gaactggcga cgcgcgagaa tttacgccta
    14221 aacgggattg atgagtcgag cgtgaagttt ctcgacagca ccgccgacta cccgcaacag
    14281 ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat tgctggaaca gcaactgcgt
    14341 gcgctgcgca aagtggtcac gtcggataca cgtattattg ccggtgccaa agcccgtgac
    14401 attcacactt ccacgctgga actgttcgaa aaagtgctcg gcccgaccac caccacgctg
    14461 gcatggaaaa aagcgcgcct gattaattgc acgttcaatg aaccgccgct ggccgatgcg
    14521 ccgcagaccg ttagctggaa gctggaaggt actgactgga ctatccataa ccatgcgaat
    14581 gtcttctccc gcaccgggct tgatatcggc gcgcgcttct ttatgcaaca tctgccagag
    14641 aatctcgaag gggagattgt cgatctcggt tgcggtaatg gcgttattgg tctgacgctg
    14701 cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg aatcgccgat ggcggttgct
    14761 tccagccgtt tgaacgttga aaccaacatg ccagaggcgt tggatcgcag cgagtttatg
    14821 atcaacaacg cgctctccgg cgtggagcct ttccgcttta atgctgtgct ctgcaaccct
    14881 ccgtttcacc aacaacatgc gctgaccgat aacgtcgcct gggagatgtt ccactacgcc
    14941 cgccgctgcc tgaaaatcaa cggcgagctg tatatcgttg ccaaccgcca tctggattac
    15001 ttccataaac tgaagaaaat tttcggcaac tgcaccacta ttgcgacgaa taataaattt
    15061 gtggtgctga aagcggtgaa gctgggacgt cgtcggtaag attgggagtt acctgccgga
    15121 tgcggcgtaa acgccttatc cggcctacgt tcagcacgtt acccccaggc cggataagac
    15181 gcggcaagcg tcgcatccgg caatgtctgc tcagttctgg aatcgcgctt ccataaaaca
    15241 aaaaatccct gtcaccatcc tctacattct cttgtttagc gtttttctac gtttattctt
    15301 ccgtcacaca gataaattca tccgttgcca atctgtcaac ggatgttatc atattgccaa
    15361 tttgtcaacg gacgtgatga agaatgcacc tgataactca aaaagcattg aaagatgctg
    15421 cggaaaaata cccgcaacat aaaacggagt tggtggctct ggggaacacg attgctaagg
    15481 gatatttcaa aaaacctgag tcattaaaag cagtattccc atctctggat aacttcaaat
    15541 atctggataa gcattatgtt ttcaatgttg ggggcaatga attacgtgtt gtagcaatgg
    15601 tcttttttga atcgcaaaag tgctacatac gtgaagttat gacgcataaa gaatacgatt
    15661 tctttaccgc tgttcatcgt actaagggga aaaaatgatt gcgattgccg acatcttgca
    15721 agcaggagaa aagctaactg ctgtggcacc ttttctggcg ggtattcaga acgaggaaca
    15781 atacacccag gcgctggaac tggtagatca tctgctgctc aacgatcctg aaaacccctt
    15841 gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg aatttgcgga
    15901 atttaatgcc atggctcaag ccatgcctgg cggtatagcc gtgattcgta cccttatgga
    15961 tcaatatggt ttaacccttt ccgatctgcc ggaaattggc agtaaatcta tggtgtcacg
    16021 cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg caacgcgatt
    16081 cggcatttct cccgccttgt ttattgatta acgcgacgtg ccgaatgcgt tgcatccggc
    16141 gcttcaaata acgatttaaa tctccagcgc cagccgtgtt ccctgggcaa ttgcccgtcg
    16201 tgcgtccagc tccatagcca catcgcagcc gccaattaaa tgcacagttt tcccgctatc
    16261 aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct gcgcagatca ccacattgtc
    16321 cactgctaat acctgcgttt cgccgttgat caccacatgc agcccgtcat cgtcaatctt
    16381 ctgataactt acgcctggga tcattttcac accccgcgag agcagggtgg tgcgatggat
    16441 ccagccggtg gttttgccta acccctgtcc cggtttgctg gctttgcgct ggagcatcac
    16501 aatttgccgt gggctacgcg ggatctgcat tccctgcggg cttaagccac cagcctgttg
    16561 taggctgctg tcgatccccc actcattaca gaacctggcg atattctggc tggtggattc
    16621 gcccggctga cttaaataca tcgccgtatc aaaaccaatc ccgccacaac cgatgatggc
    16681 aaccttgttg cctaccggcg ctttgtcgcg cagtacatcg agataactca ataccttcgg
    16741 atgatcgatc ccgtcgatag gcggaacacg cggcacgatc ccactggcga ggatcgtttc
    16801 atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg agtttcagcg tcacgcccgt
    16861 cacttcgatc atccggcggt agtagcgcag cgtttcgtaa aactcctctt tgccggggat
    16921 ctgtttggcg atattaaact gcccgccgat ctcgctgtgc gcatcaaaca atgttacctg
    16981 atgcccacgc gccgccgcgt taatggcaaa cgccagccca gcaggtccag cgccgaccac
    17041 cgccagattt tttttctgca cggcgggaag gattggcatt ttggtttcgt ggcaggcgcg
    17101 aggattcacc aggcacgagg tgactttgcc aacgaagatc tgatcgaggc agccctggtt
    17161 gcagccaata caagtgttga tctcatcggc tcgccctgat tgcgcttttg acagcagttc
    17221 cgcatccgcg agaaacggtc gcgccatcga tactatatcg gcatcgccgc gcgagagaat
    17281 atcgtcggca acctgcggat cgttaatccg gttggtggtt accagcggca gcgagacgtg
    17341 gcctttcagt ttgcgcgtga cccagctaaa tgcgccgcgc ggcacgggcg tggcgatggt
    17401 cggaatccgc gcttcgtgcc agcctattcc ggtgttgata atggtcgcgc ccgccgcttc
    17461 aatggcctgc gccagttcta ccgtttcggc aaaagtgccg ccgccttcga ctaggtcgag
    17521 catcgacaac cggtaaataa taatgaagtc gttgccgacg cgttcgcgca ccgtacgcac
    17581 tacttctacg gcaaatcgca tccggttgcg gtaatcgccg ccccactggt cactacgctg
    17641 attggtgcgc agcgtcagaa attcgttgat caaataccct tcagagccca tcacttcgac
    17701 gccatcgtat ccggcctccc gcgccagttg cgcgcagcgg gcgaaatcgt cgatcagttg
    17761 caggatctct tcatgggtga gttcgtgagg aacgaaacgg ttgatggggg cctgcaatgc
    17821 ggacggtgcg accagatgcg gttggtagct gtagcgcccg gtatgcaaaa tttgcagggc
    17881 gattttgccg ccttcctgat gtacggcttc ggtaatggtg cgatggtgtg ggatctggct
    17941 ggcgtcgttg agcattgcgc caccttccat gccaacgcct gttaaatctg gtgcaatgcc
    18001 gccgctgaca atcagcgcca cgccgtgacg ggcgcgttcg gcataaaacg cagccagccg
    18061 ttcagcaccg tccgggtatt cctccagccc ggtgtgcatt gaacccatca acacgcggtt
    18121 ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc gacgggtagc tcataaaatt
    18181 gtccagtatg taaaataatt gttatgtagt cggatgagtt ctaatttagc cgtcgatgga
    18241 gaaaagggaa aaggggagtg cggggtttgt gatgggattc aaaaagaata cgcccggtgg
    18301 taacgccgga tgcgacgctt gccgtgtttt atccggcttg ggggattgca catgtagggc
    18361 ggataaggcg ttcacgccgc atccgccagc cgttgcaaat tactgcttac ggaaaaaatc
    18421 gttatacaac atatacaaat gagcggcact ccaggagaaa tttggtgcgc cttgctgcgc
    18481 gcctgtcagc gggttgtaat tctcctgaat tgggccatcg gcggttaatc ctttggcgtg
    18541 cctgaagaac gtatccgcca gcttcagggc atcatcacga taaccgtaac gctccatccc
    18601 tttcagacca aaccagaact gatccaccca tacgcgcccg cgccagtaga tatcagcgcc
    18661 aaaagccgga ttggttaacg ccgccgttcc cagcggaaca aatgtgttga actctttagg
    18721 atcgagcatc accttcacca ccgcgtcggc attggcctgc gttgccgcac cgttaaacag
    18781 cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg cgcagccgtt
    18841 cgccagtggt ttatcttcaa tacgtacgtc atagtagaac tgggtagtcg ggtcgaacat
    18901 acaggtgttg atgtagtccg cgagctgctg tgccaactgg cgatagcgtt tggcctcttc
    18961 tggcttaccg aggatggttg ccatctccgc cagataatgg ttatcgctgt acatatagct
    19021 ggcctgatcc accgactcct gcaatagcga gtagcccagc aacgttccgt cctgactgcg
    19081 gttttcggcg aatttcaccg tccagtcgct acgtttgccg ccattagcga catatttatc
    19141 caactgttct ttgtcgataa aaccaaagac ggcggcatca tcacgccccg attcccacga
    19201 cgcagcgacc tgtgccggaa tttccagact gtcgtactgg cctttctcca ccacgcgggc
    19261 gtagttgttc agcccagact gcgtctcttc tttatcgcct tttttcaccg taaacagcat
    19321 ctcgccgctc tcggtgttgt gggctttgtc gcgggtcgcg ccatattccg gcaccccgtt
    19381 gccgttatga tcgcggttac gtaaccacca gtcgtgatag gccaccagtt tcgggtacat
    19441 ctctgccaac caggctttat cctgggtgac gttgtatact tccatcaccg accaggcggc
    19501 aaggctgggt ttggtattac gttcgttcca gttgccgcca tcgccaccac gctcggggct
    19561 aaggttccac gctatcaggt cggggacaaa gcccacatcc tgcggacgca cgctgtcgcc
    19621 aggctggatc tgccaggaga agaccgcgcg gatattctct ttggcgatat ccggattgaa
    19681 atgcgccatc gcaaacgcct gcttccaggt atcccacggc caggtctgat tgccggagaa
    19741 ccagcgtccg gtcaccgacg gcgtgacggt gttataattc accgcaccgc ccggtgagcg
    19801 ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt ccggcgtcgc
    19861 atccggattg gttaaccctt tcttcaggta ttcttcccag cgttgctgcg aggcggtgag
    19921 ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc taacttcctg
    19981 agcggtcagc agatgggaat aggtggtgta gagcgtggtc gaaccgttga tatgcgcctt
    20041 actggtaaag cgattaccgt tgatttcagt ctgcaccggc agggatttat gcacctgata
    20101 ttccgattcg ccggaggtca gcagatccca ggtggcgcgc actttgccaa aggtgacttt
    20161 caggccatca cgggtggcgc tgattttgcg ctgatagtca gggtattcgc cagcaatggt
    20221 tttatcggaa agcggtttcc cttctttcgc ttccagtttt tccagcagtt cgccatccca
    20281 caccagatcc agcggtttat tgctgatgat tttagtttcc agtagtgacg tgcgcggcgt
    20341 ggcgaagcgc agagtcattt cgacctgcac atcttttgct gtcagttttt gcaccagcgc
    20401 gccgggaata ctgtatgcct ccagcgtgaa gttgactttc ttgccgtctt gccagacagt
    20461 caggcggtcg aaattgctgg ccataaagtt gatgtattct tccgtcagca gcgcaacgcc
    20521 cggaaagccg cccatggtgt tcgggccgtc tggcaacaga tgaccatgcc aggctccgag
    20581 atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg taatccttca tgtactgcgg
    20641 cgcaccagta cggttaatca cgtttttata attgtcggcg ttagcggcat gagcggaaaa
    20701 acttatcagc agagcgcagg ttactggcgt taaaatagtt ttgattttca tgagattaca
    20761 gtccttatta ccagtagaaa tattgcatca ggaagatttg gtcgctggga tcgttagagg
    20821 catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa acgtccgccg ccatattcgt
    20881 accagatacc aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca gggcgatgtt
    20941 tggcgtaggt gtaagcggtg gagagataaa cgccttttaa cgcttcatac atggcgctca
    21001 ccatcatccc gctttcactt cccggtacgt ccagcccgtc ggcacgtccg gtatggtgcc
    21061 aggcgcgagc ggcaaagtta ggggtaaaca ggcccgtgag atagagttgt cctgtgccgt
    21121 cggtaatttc cagaccattc atccaggtga tgccttcggc aaggtcatac tggatgtaac
    21181 cgttcatcca ttgccggata ggtgtatttg tcgtcgtagc gatcgtactt gccaaaatgt
    21241 agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg cggtgacgct ataacgaaag
    21301 ttgccgctta agttctgtac cttcagcgcg gtcataatgt cgcgggtatt gggaatgacg
    21361 tagcccaaat cgggtgtgaa gtcgccccac cattgcaggt catccagcga ggaatcctga
    21421 cgcagactga gcatgatttc ggtgccatca ttggtacgat aaccgccata gaaacggtta
    21481 atcccacctt cgaaaccacc ccagctatgg tcaggcacct aggcattacc gtcgtggtcg
    21541 gcctgcacgg tccagccttc gccgtaaatc aggccgaacc aggagccgta cttcatctcc
    21601 aggccacctt caatataggt gccgtcgctg tatttatgtt tattctcacc ctctaaatcc
    21661 atatatccac caagccctaa ttcgccgtaa aaacgaaatg aaggagaaga tgtcgaggta
    21721 attgcgggtg tttttgtgtc gtcatgttct gccggacgtg cttcttctcc tgccgcagaa
    21781 aaggcgtagc aaaacggcag agcaagtaat gctctgcatg gtgcagtgat taatttcatt
    21841 gtgttaatcc atgagctaaa gaaaaagtac agccttttat cgttgtaatt ataaaaatat
    21901 aaaggcagaa actattttcc ctctcctcat ccaggaagag ggaaaatgcc tgctcatttt
    21961 ttattagtct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc gctcttcagc
    22021 cgttaatccc tttatatatt tgctgtattt ccaccacgca aagccgagga agataacaat
    22081 accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga atgtcgaagc
    22141 gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac cggtgcggcg
    22201 ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat tcaggtaagc
    22261 cagcatgatg aataacggcg gaagcatgga cgctgcggcg gtcatattaa taatggtatt
    22321 catcagatcc tgcacggtat tggaaccgag catcgggata atcatcagcg ggatgacgat
    22381 cagaaactgg atccatgctg cgcgcgccgg aacgccgttt tcgttaagtt cgacggtttt
    22441 cttaccaaag atgccttccg ggatttcgga gaagaaaatt ttcaccggcg ttgcggtcca
    22501 catcagcagg gaaccgaaca ttgcggtaaa ggacaccaga ccgacaaagc gattcatcaa
    22561 cgcttccggc agaccaaaat acgccgccat gccgtggaat acctgcaccg atccgccggt
    22621 aaatttcaac tctttgctgc tgacgaagac gttaatcagc accgaggaga cggaatacag
    22681 tacgccgata aaaatcccgg cgaggatgat cactttaacg aacgatttcg aaccgccttt
    22741 gacgtcgtta acgtacaccg cgacggactc cgcgccacct gcggccataa agatccaggt
    22801 ggtaacgccg aggaacgccc agttgaagtt cgggatcatc gcgtcaacgg tgatcggatc
    22861 ggcaggctgt acgccgccaa ccagtgccgt acctgccagt aaaatgtagg agagcgttaa
    22921 cagcagcatc agcgttgaag tgacggaggt aattggcccc agcattttcg ccccgttggt
    22981 ggaaacccag gtggagaagg cgaacagcac catactgata acggtggtgg caaccggcgt
    23041 cataatatat tcgtagccga ggaaggcata cgaagcataa gcaataacgc gcggcaacag
    23101 cgaggtgaaa aagaacagat ttacgaacca gtaagtgtag gcagtaataa atgcccaacg
    23161 tccgcccagc gaacttttta cccacgcgta gacaccggct tctgagtttt tatttaacga
    23221 aacaaattct gcgatgatca gacagaaggg aataaaataa aaaatcgtcg cgaggaaaaa
    23281 catcggtgcc gaggccagtc caagctcaat attattattg ataacgttgt taaagctgta
    23341 aacggcggca aaagtcagcg agagcaagcc gaatttgccg attgtattac gtttggtatc
    23401 agacataaca catctccgaa aatgtgtagg gtagcggtgg cggcaaactc cttttgccgc
    23461 cgtagttgtt atttgttatg gaaataacca tcttcgatgg tgactttgag aaccactttt
    23521 ttgaccgcgt tattgcagat aaaccgatac gcttcatgga tatcgcaaat aacgatttgc
    23581 ccttcgtgga cctctacggt ttctccgcag ccttttaaat attcacggtc agtttcatca
    23641 cgataatatt ccactacctg taatgtctct ttcggcgcat attcaatttt ttgctgccct
    23701 tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg taaatagcgc atcggtcgcg
    23761 gagtcatttt ccacacggta ggtcaatgaa tcaccgatgg agtgggcgac gccaggctga
    23821 atgttgtcga tattttcaat cgcttcaacg cagcgttgcc acttcttgcc agaggcgtaa
    23881 atctggcgga actgttctaa gttatcgatg atcctcattg cttattctcg ctgtgcaaat
    23941 tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag gctttgcgcg gtagcttctc
    24001 cgccagaaac cggcagcaac gtaaagccgt agctgaagtc acggaaccag acgcgccagg
    24061 agtccagcac ctcgctgccc caggagttgg agccgaggcc aagtagctgg tggtcgaggt
    24121 tcagggtgat gtcatcactg cgctgcagct cgttacagtg ctgggcagcg tggatgtttt
    24181 cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg cggaaccacc agcagaccgt
    24241 tgccgtggcg gttagtcagt gccgtccagc ggacatgctg acggttgccg ttgttctgcg
    24301 ggaagggata gttctcgaac atggcatcga cggtgctgcg ccagatatcg atgatgttag
    24361 cctgctggct gtcggcgtag ttttcgcccg gtccacgacc gtaatacgcc acctgatcgt
    24421 attcgccgtt aatccccatg gtgaaaccga tgcacggaat gatgtgcgga tagtcgccgt
    24481 aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc agcgatgcgc cagatgtagg
    24541 tacagcgcat cccgaagtca aacaccggcg gtgcaataac tgtgcggctg atgatcaaca
    24601 cttcaccatc gctctgttct acggcaaagt cgcgcagatg ttcctgcatg atctgcaaat
    24661 gattcggctg ccacaaccct tcgtactcct gcttgtggtt gtcgatcatc ggcttgaaga
    24721 agttgatctt tggctcgcgg gtcagcagcg actcgccatt tacctgccag gatgtcggtt
    24781 tgccactcat ttttgagaag gtgatcgcga agttgtagcc gcgaacggtg cagctcaaac
    24841 gatcgtcttc cagcgtcagc ggacgcgcat tatttggtgc gaaaggcact ggctgcgcgg
    24901 tgttttcctt cagcgggaac tgataagtgg cgatagaatg cccggcttcg ctgtagcggg
    24961 tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc gcgggcgtcc agctgcggca
    25021 gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc gcgcagttta atctgctgcg
    25081 tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag ggtgtagtca tcaagcgtgg
    25141 taaaccacag tttattttcg actttcaact cgccgcgagt cagcgcgtgg atttttaccg
    25201 gcgcgataac ctgtttgtac tctttcaggc ccgatcccgg cgtctgatcg gaatagatca
    25261 aaccatcaag acagaagtta tagttgttgg gatagtcgcc gtagtcgccg ccgaatttat
    25321 accagacatt gccgtggtcg tcctgtgcct ggatcccgtg gtcgcaccac tcccagacat
    25381 aatgaccctg aatgcaatcg tgcttataga agacgttctg gtactccgtc agcccgcccg
    25441 gtccgttccc catcgcatga gcatattcac agatgatgcg cggcttcgga tgcgggtatt
    25501 caccaaactc attcatcagc ggcacgcggg tgtacatggt ggaaataata tcgaccactt
    25561 cagcatcgcg atcttcttcg taatgaacca gtcgcgtgtc atccagcgct ttcgccgcat
    25621 ggtacatcgc gcggatgtta cagccatagc cggattcatt gcccagcgac cagatgatga
    25681 tcgacggatg gtttttctgt gcgtggatat ggcgaacaat gcgctcgacg tagacttttt
    25741 cccactgcgg atcgtcggta atacggctga tatcgccgac attagcaaag ccgtgcgatt
    25801 cgacgtcggt ttccgccatc acaaacaggc cgtagatatc acacagttcg taaaaacgcg
    25861 gatcgttcgg gtagtgagcg gtacgcacgg agttgatatt gtgctgcttc atcaactgga
    25921 gatctttctc gacgcgatcc attccaacgg cgcggccttt gcgatgatcg ttgtcgtgac
    25981 ggttgacgcc gtgcagcatc acataacggt tattgatcca gaacagaccg tcgcgcactt
    26041 tgatatcacg gaagccaacg cgttgtggca ccacttccag aacgttgccg ttggcgtctt
    26101 tcagcgtcat gaccagatgg taaagataag gggattctgc tgaccattgc tgcggctgtt
    26161 cgacagtaaa agcaaagctg gcgctggtca gtttttcaat tgccaaatga tcaatggcgc
    26221 tgctgtgcac cacgcgttcg ccatcaaaca gggtatattc cagcgtcgtg acgacagggg
    26281 aggcggcgag attttccagc accacttcgc aggaaagcgt ggcatcgcaa taggcttcgt
    26341 caaagtcggt acgcacagtg aaatcgttaa tatgcgttag gtgttttccg accagataaa
    26401 catcgcggaa gatccccgcc gaccaccaca tatcctggtc ttccacgtag gtagagtccg
    26461 cccactgcat cacgcgcaca cacaacaggt tgtcgccggt tttaaccatc gcgctgatgt
    26521 caaactctgc ggtcaggcga ctgcccttgc tgaaacccac atactgaccg ttaacgtaga
    26581 cttcaaaata ggtttcgacg ccgtcaaatt taatcagcgt ctgtttaccc tgccagccgt
    26641 cgctgagggt gaaaatacgt tgataggcac cggttgggtt atcgctgggg acaaacggca
    26701 catcgatggg gaacggaaaa ccttcgtcgg tatattgcag tttgccgtga ccttccattt
    26761 gccacatggc ggggacggta atatgccccc agtcagccat taactcagag gtgaaggctt
    26821 ctggtacttg cagcggatgg tcaaaaaagt ggaaattcca ctgactgctt aagggcagaa
    26881 acaggctgct ggtttcgcgg gcaaaggtac gcgcttgcgc aacagaatca tatgaaaaaa
    26941 agtacgcacg cggcgcaagt cggttttcgt gggtgagctg aatgttttcc cagcgattca
    27001 tagggcctcc cggtagagag aaatcggaac cattcatggt gatggtagtt tgtgtaattg
    27061 ctgggggttt agtaaaattt ttactaaaaa gcagcaggta aatgcattta ctttaactgg
    27121 atcacgaaaa taaacggcag cactgtccgg caaaaatgat tgccgaacag tgtgtaaggg
    27181 gatttaacgg gtcgtgccgc gcagttttaa tttgctggga acgaagacta gcagcggcag
    27241 cgcgcgacca tcgcgggctt tttcatacac caggttaacg ccctgacttc ccatcatctc
    27301 ggaatggatg cgcacggtgg agagcggcgg aaaggtaaat cgcgcggtgg ggatatcgtt
    27361 aacgctgata agcgaaatat cttgtgggat gttcaggcca cgttcatgaa ttgcccgcag
    27421 tacgccgata gcaatggaat cggaagcaac aaacagtgcc ttcggatagt cttcccgcgc
    27481 cagcatttgt tttgccagtt cataacccga cgaactggaa aaaccgccgc gccagatatc
    27541 ctcttcgcgt accacttgtt tcagtcggcc atattccgca aaggcgacct cacgaatatc
    27601 cgccttgcca ggctcatctt caccgccaat aaaaccaata cgattaacgc cctggttgat
    27661 atagaagtcg atgatttctt tactgatgcg tgccagatcg atatccaccg catcgtaacc
    27721 gctgccgggt tcgtgaaagt cgataaaaca gatattgtcg gtcaacgcgc tggcagcggc
    27781 gcgcagggcg ggcgtgggtt tgccgacaat taaaataccg gtgacgtttt taatgtctgg
    27841 taatccgttg tgttcataac agttggtcag ttcgatcgcc agcttttcgc actgagtttc
    27901 aatgccgtgg cggatcgcca gatagtaagg atcgttgatc tccagctcct gctggtagct
    27961 gtagatagcc agaatatggt gttggttgac tgcgcctgtc tgtagtttac gggcgctact
    28021 ggtcttgtac tccagctttt cggcgatctc gagaatgcga tgtttcgtct cttctttcac
    28081 attcaatgtc ggatcgtcat ttaagaccct ggatactgtc gccagggata cgccagcttc
    28141 gattgcgatg tcttttaatg ttgccatgtt tacctttttt actttccctg atgtgcgccc
    28201 ccattgtaga ggaagtgctt atcgggtcac ctgttttcag taaataaagc gcgtttcttt
    28261 gacgctgtaa cggtaacaca gcggcttagg aatgtgatca cgcctgcatt ctccattttt
    28321 agcctacagg attgagaaaa acttttctat agtttagtaa aattttactc gaagggagga
    28381 agcaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat
    28441 tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg ttgatgtggg
    28501 gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga ggagctgatg
    28561 gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg tgaaacgtcg
    28621 gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt gttaacgccg
    28681 gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc gacgtcgtat
    28741 cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc cagcgcctta
    28801 cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg cggttcaatg
    28861 cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg gaacatctcg
    28921 ctggcaaagt tatagccgat ttcgttatca acaaactcaa tcgccatcaa catcccttta
    28981 ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa accgtccagc
    29041 aacatatcgc ctttttgctc agcctgagcc ggtaagttct gctccagcaa cacattgatg
    29101 gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt atgcaggaat
    29161 gggttgtcga acagaactga aaacacctct tcagtggcga tggtcgcgcc aatcggcatc
    29221 acgccgccgc cgagggcttt tgccagacac agaatgtccg gctgaacgtt ctcatgttcg
    29281 caggcaaaca tcttgcccgt gcgccccatg cccgtttgta cttcatcgag gatcatcagt
    29341 gcgccgaact catcgcacag cttacgcacg gcggtgagat agcccggcgg cggcagaatt
    29401 acgccacctt cgccctgaat cggttcgagg atcaccgcag ccacatcatc accggttttt
    29461 ttgcactcat taagagccgt gcgcatggct tcgatattgc caaacggcac atgacggaac
    29521 cctggcagca gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc cgacagcgcg
    29581 ccaagtgatt taccgtggaa cacgccgctg gtggcaataa aagtaaattt gccgcgcggc
    29641 gactgataag ccttcgccag cttcagcgcc gcttcaacgg actcggtgcc gctattacag
    29701 aagaagctgt atttcagttt accgggcgtt agcgcagcaa gggttttcgc cagcatagcc
    29761 cgtaacggat cgagtagctc ctggctgtgc agtggttgtt tcgcaagttg attctgtacg
    29821 gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc tcccaggcag
    29881 tcgataaact cctgtccctg ggtgtcgaca agcgtattta aacctcccgc ttgccactct
    29941 acggctccgt aatccccgcc ggcggtaaca gatttgcgat actctaaaaa ccccggattg
    30001 acatgctctt tgaagtattc aatcacctct cggttaagtg ctttcatctc ctcatgatcc
    30061 agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc cgatgcgctc
    30121 gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata ccgatttaag
    30181 tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa cacaaatgcg
    30241 gttatcattt gtgcaatatt taatgctatc cgttaacatt tgttgttatt ttgcacggtt
    30301 tttgtcgggt atggatttga tgattgctgc gcaaatgcac gttttcaggg caataattgc
    30361 ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa gtattttaaa
    30421 tcaaagaatt agatttgtgc tttaatcgca aattgcgatc taaatcaaat taatcggtta
    30481 aagataaccg cagcggggcc gacataaact ctgacaagaa gttaacaacc atataacctg
    30541 cacaggacgc gaacatgtct tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg
    30601 acgataccac tctgatgtcc actaccgatc tgcaaagcta tatcactcat gctaatgaca
    30661 cttttgtgca ggtgagcggc tttaccttgc aagagttaca agggcagccg cacaacatgg
    30721 tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag
    30781 gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat tattgggtgc
    30841 gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta
    30901 cccgggcgac ggatgaagag attgcggcgg tggagccgct gtacaaagcg ttgaactccg
    30961 gacgtaccag taagcgtatt cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac
    31021 tgccttcatt accgcttcgc tggcggacgc gtggagtgat gaccctgatg tttatcttgc
    31081 tggcggccat gctttggttt gttgctgccc cggtggtgac gtatttcctc tgtgtgttag
    31141 tggtattgtt ggcaagcgct tgttttgaat ggcagattgt gcgcccgata gaaaatgtcg
    31201 cccgtcaggc actgaaggtg gcgaccggag agcgtaatag tgttgagcac ctgaatcgca
    31261 gcgatgagct ggggctgaca ttacgcgcgg tagggcagct tggcctgatg tgccgttggc
    31321 taattaacga tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag acgctggcga
    31381 aaggcaccga tgaactgaac gaacataccc agcagacagt tgataacgtt cagcaaacgg
    31441 tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg gcgtcggctg
    31501 ccgataaact ttctatcacc gccagtaatg cggcagtgca gggtggggag gcgatgacca
    31561 cggtgatcaa gacaatggac gatatcgccg acagtaccta gcgcattggc accattactt
    31621 cgctgattaa cgatattgcg tttcagacca atattctggc cctgaatgcg gcggtggaag
    31681 cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt
    31741 tagccagccg cagcgctaat gctgccaacg atattcgcaa gctgattgat gccagtgctg
    31801 ataaggtgca atccggttcg cagcaggtac acgccgccgg acggacgatg gaagatattg
    31861 tggcacaggt gaaaaacgtc acccagttga tcgcccagat tagccattca acgctggaac
    31921 aggccgatgg gctttccagc ctgacccgtg cagtggatga gcttaacctg atcacccaga
    31981 aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca
    32041 gccgactgga agacgcggtg acggtgctgc attaatcggt atgccggatc cggcggttgg
    32101 agcacaatgc ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt
    32161 gtaggccgga taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc cactggcgga
    32221 tgcggcgatt aatccagttg cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc
    32281 ttttttattt gtgcatcact gatatcgctc tggttgacgc gtaaatccag caccgcttta
    32341 aaattatcca gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc
    32401 gctttgatgc gttcttcaat catctccact tgttcgcggt tttcttccag ccactgcgcg
    32461 ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct cttcgcggat ggtaatcagc
    32521 gactgctcct gcagaaaatc cagcgtcggg tagatgacgc ccgggcttgg ggtgtaattc
    32581 ccctgggtta gattctcaat cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag
    32641 agaatatcca gaatcaccag acgcaattca ccgtgaccaa agaaacgttg ccgacgaccg
    32701 ccgccgcgac cgtggcgacc accgcaacat tgaccgtgtt catgctggtg accgtgtccg
    32761 cagtgctcgt gttctgactt ctcacctttg cagcagccct catggcgtgg ctggccttca
    32821 tgtttacaac acccttcgtg atgatggctc atatcagcct ccttttgctt gattagatat
    32881 atctaattta tatctgatgt gagcattact gcaagtacaa aaatgatttt taaacgtgga
    32941 agcacctcac atctaggccg gataagacgt tcacgccgca tccggcagca ttgctatggc
    33001 actgaatgcc ttcacatact tcagacatat cagttctaca aatcgcttgc atttatcatg
    33061 attaacaatc attatcattt gcgaatttta tttagatata tctgattacc atcacgaagg
    33121 cgataacaat gaataacacc ccccgctacc cgcagcgcgt tcgcaatgat ctgcgcttcc
    33181 gtgaactgac tgtgctacgc gttgagcgca tcagcgccgg ttttcagcgc attgtccttg
    33241 gcggcgaggc gttggacggt tttacatcgc gcggctttga cgatcacagc aaactcttct
    33301 ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagagc atcgtctggc
    33361 cggaaggacc acgcccaccg tcgcgtgact atacgccact gtatgacgaa ctacgccatg
    33421 aactggcgat cgatttcttt attcacgacg gtggggtcgc cagcggctgg gcgataaact
    33481 tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt atcagctgta
    33541 tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt tgagcaaact
    33601 tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct gtcaggatta
    33661 tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc aggcggtaga
    33721 tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga taaccggcga
    33781 aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg acccacagcg
    33841 ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga aaaaggctga
    33901 cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa ccagcgacca
    33961 agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag cggattataa
    34021 agtaactccg tgccgcaatc cagccattac
//
LOCUS       DAJTYL010000032        34050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYL010000032 DAJTYL010000000
VERSION     DAJTYL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.032"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            364..2649
                     /gene="nrdA"
                     /locus_tag="OO572_001500"
     CDS             364..2649
                     /gene="nrdA"
                     /locus_tag="OO572_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6660547.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2883..4013
                     /gene="nrdB"
                     /locus_tag="OO572_001501"
     CDS             2883..4013
                     /gene="nrdB"
                     /locus_tag="OO572_001501"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6660548.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4013..4267
                     /gene="yfaE"
                     /locus_tag="OO572_001502"
     CDS             4013..4267
                     /gene="yfaE"
                     /locus_tag="OO572_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6660549.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4321..4971)
                     /gene="inaA"
                     /locus_tag="OO572_001503"
     CDS             complement(4321..4971)
                     /gene="inaA"
                     /locus_tag="OO572_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6660550.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5150..5392
                     /locus_tag="OO572_001504"
                     /pseudo
     CDS             5150..5392
                     /locus_tag="OO572_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5434..6510)
                     /gene="glpQ"
                     /locus_tag="OO572_001505"
     CDS             complement(5434..6510)
                     /gene="glpQ"
                     /locus_tag="OO572_001505"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6660551.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6515..7873)
                     /gene="glpT"
                     /locus_tag="OO572_001506"
     CDS             complement(6515..7873)
                     /gene="glpT"
                     /locus_tag="OO572_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6660552.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8146..9774
                     /gene="glpA"
                     /locus_tag="OO572_001507"
     CDS             8146..9774
                     /gene="glpA"
                     /locus_tag="OO572_001507"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6660553.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9764..11023
                     /gene="glpB"
                     /locus_tag="OO572_001508"
     CDS             9764..11023
                     /gene="glpB"
                     /locus_tag="OO572_001508"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6660554.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11020..12210
                     /gene="glpC"
                     /locus_tag="OO572_001509"
     CDS             11020..12210
                     /gene="glpC"
                     /locus_tag="OO572_001509"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6660555.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12403..13353
                     /locus_tag="OO572_001510"
     CDS             12403..13353
                     /locus_tag="OO572_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6660556.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13394..13864)
                     /locus_tag="OO572_001511"
     CDS             complement(13394..13864)
                     /locus_tag="OO572_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6660557.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(13919..15121)
                     /locus_tag="OO572_001512"
     CDS             complement(13919..15121)
                     /locus_tag="OO572_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6660558.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15208..15750)
                     /locus_tag="OO572_001513"
     CDS             complement(15208..15750)
                     /locus_tag="OO572_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660559.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16029..16454
                     /gene="nudI"
                     /locus_tag="OO572_001514"
     CDS             16029..16454
                     /gene="nudI"
                     /locus_tag="OO572_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6660560.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16493..17095)
                     /locus_tag="OO572_001515"
     CDS             complement(16493..17095)
                     /locus_tag="OO572_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6660561.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17403..18542
                     /gene="arnB"
                     /locus_tag="OO572_001516"
     CDS             17403..18542
                     /gene="arnB"
                     /locus_tag="OO572_001516"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6660562.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18546..19514
                     /gene="arnC"
                     /locus_tag="OO572_001517"
     CDS             18546..19514
                     /gene="arnC"
                     /locus_tag="OO572_001517"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6660563.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19514..21495
                     /gene="arnA"
                     /locus_tag="OO572_001518"
                     /pseudo
     CDS             19514..21495
                     /gene="arnA"
                     /locus_tag="OO572_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21492..22382
                     /locus_tag="OO572_001519"
     CDS             21492..22382
                     /locus_tag="OO572_001519"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6660564.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22382..24034
                     /gene="arnT"
                     /locus_tag="OO572_001520"
     CDS             22382..24034
                     /gene="arnT"
                     /locus_tag="OO572_001520"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6660565.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24031..24366
                     /gene="arnE"
                     /locus_tag="OO572_001521"
     CDS             24031..24366
                     /gene="arnE"
                     /locus_tag="OO572_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6660566.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24366..24752
                     /gene="arnF"
                     /locus_tag="OO572_001522"
     CDS             24366..24752
                     /gene="arnF"
                     /locus_tag="OO572_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6660567.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24746..25018)
                     /gene="pmrD"
                     /locus_tag="OO572_001523"
     CDS             complement(24746..25018)
                     /gene="pmrD"
                     /locus_tag="OO572_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6660568.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25123..26478)
                     /gene="menE"
                     /locus_tag="OO572_001524"
     CDS             complement(25123..26478)
                     /gene="menE"
                     /locus_tag="OO572_001524"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6660569.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26475..27437)
                     /gene="menC"
                     /locus_tag="OO572_001525"
     CDS             complement(26475..27437)
                     /gene="menC"
                     /locus_tag="OO572_001525"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6660570.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     SSPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27437..28294)
                     /gene="menB"
                     /locus_tag="OO572_001526"
     CDS             complement(27437..28294)
                     /gene="menB"
                     /locus_tag="OO572_001526"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6660571.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28309..29067)
                     /gene="menH"
                     /locus_tag="OO572_001527"
     CDS             complement(28309..29067)
                     /gene="menH"
                     /locus_tag="OO572_001527"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6660572.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29064..30734)
                     /gene="menD"
                     /locus_tag="OO572_001528"
     CDS             complement(29064..30734)
                     /gene="menD"
                     /locus_tag="OO572_001528"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6660573.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30823..32118)
                     /gene="menF"
                     /locus_tag="OO572_001529"
     CDS             complement(30823..32118)
                     /gene="menF"
                     /locus_tag="OO572_001529"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6660574.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32197..32502)
                     /gene="elaB"
                     /locus_tag="OO572_001530"
     CDS             complement(32197..32502)
                     /gene="elaB"
                     /locus_tag="OO572_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6660575.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32557..33018)
                     /locus_tag="OO572_001531"
     CDS             complement(32557..33018)
                     /locus_tag="OO572_001531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6660576.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33083..34000
                     /gene="rbn"
                     /locus_tag="OO572_001532"
     CDS             33083..34000
                     /gene="rbn"
                     /locus_tag="OO572_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6660577.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         8248 a         9182 c         8504 g         8116 t
ORIGIN      
        1 atgtgatgac tcgtgcttag atcaattttt gcaatcatta gcaaaaagat taataaacca
       61 tctatatcaa tttatccaac ctattctgcc gttcaagaaa tcgccgaaca gttattttta
      121 acaaattttt ctcttcccat tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa
      181 agccactttt tccttcctga gttatccaca aagttatgca cttgcaagag ggtcattttc
      241 acactatctt gcagtgaatc ccaaacatac cccctatata tagtgttcta agcagcttcc
      301 cttactacag gtagtctgca tgaaactatt gcggaaagaa ttccaaaaac aggtacgaca
      361 tacatgaatc agaatctgct ggtgacaaag cgcgacggta gcacagagcg catcaatctc
      421 gacaaaatcc atcgcgttct ggattgggcg gcagaaggac tgcataacgt ttcgatttcc
      481 caggtcgagc tgcgctccca cattcagttt tatgacggta tcaagacctc tgacatccac
      541 gaaactatta tcaaggctgc cgcagacctg atctcccgtg atgcgccgga ttatcagtat
      601 ctcgccgcgc gcctggcgat cttccacctg cgtaaaaaag cctacggcca gtttgagccg
      661 cctgcgctgt acgaccacgt ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg
      721 ctggaagact acacggaaga agagttcaag cagatggaca cgtttatcga tcacgaccgt
      781 gatatgacct tctcttatgc tgccgttaag cagctggaag gcaaatatct agtacagaac
      841 cgcgtgaccg gcgaaatcta cgaaagcgcc cagttccttt atattcttgt tgccgcgtgc
      901 ctgttctcga actacccgcg tgaaacgcgc ctgcaatacg tgaagcgttt ttatgacgcg
      961 gtttccacat ttaaaatttc gctgccgacg ccaatcatgt ccggcgtgcg taccccgact
     1021 cgtcagttca gctcctgcgt actgatcgag tgcggtgaca gcctggattc catcaacgcc
     1081 acctccagcg cgattgtgaa atatgtttcc cagcgtgccg ggatcggcat caacgccgga
     1141 cgtattcgtg cgctgggcag cccgattcgc ggtggcgaag cgttccatac cggctgtatc
     1201 ccgttctaca aacatttcca gacagcggtg aaatcctgct ctcagggcgg tgtgcgcggc
     1261 ggtgcggcaa cgctgttcta cccgatgtgg catctggaag tggaaagcct gctggtgttg
     1321 aaaaacaacc gtggggttga aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc
     1381 aacaaactga tgtatacccg cctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc
     1441 gacgtaccgg ggctgtacga cgcgttcttc gccgatcagg aagagtttga acgcctgtat
     1501 accaaatatg agaaagacga cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc
     1561 tcgctgatga tgcaggaacg tgcgtctacc ggtcgtatct atattcagaa cgttgaccac
     1621 tgtaataccc atagtccgtt tgatccgacc atcgcgccag ttcgccagtc taacctgtgc
     1681 ctagaaattg ccctgccgac caaaccgctg aacgacgtca acgacgagaa cggtgaaatc
     1741 gcgctgtgta cgctgtctgc tttcaacctg ggcgcaatta atagcctgga tgaactggaa
     1801 gagctggcga ttctggcggt tcgtgcactt gacgcgctac tggattatca ggattacccg
     1861 atcccggccg ccaaacgtgg agcgatgggg cgtcgtacgc tgggtattgg tgtgattaac
     1921 ttcgcttact atctggcgaa gcacggtaaa cgctactccg acggcagcgc caacaacctg
     1981 acgcataaaa ccttcgaagc gattcagtat tacctgctga aagcgtctaa tgagctggct
     2041 aaagagcaag gcgcgtgccc gtggtttaac gaaaccactt acgcgaaagg gatcctgccg
     2101 atcgatacct ataagaaaga tctggatgcc atcgctaatg agccgctgca ttacgactgg
     2161 gaagcattgc gtgaatcaat caaaacgcac ggcctgcgta actccacgct ttctgccctg
     2221 atgccgtccg agacctcttc gcagatctct aacgccacta acggtattga accgccgcgc
     2281 ggttacgtca gcatcaaagc gtcgaaagac ggtattttgc gccaggtggt gccggactac
     2341 gagcacctgc acgacgccta tgagctgctg tgggaaatgc cgggtaacga tggctacctg
     2401 caactggtag gtatcatgca gaaatttatc gatcagtcga tctctgctaa caccaactac
     2461 gatccgtcac gtttcccgtc aggaaaagtg ccgatgcagc agttgctgaa agacctgctc
     2521 accgcctaca aattcggggt caaaacgctg tattatcaga acacccgtga cggcgctgaa
     2581 gacgcgcagg acgatctggt accgtcaatc caggacgatg gctgcgaaag cggcgcatgt
     2641 aagatctgat attgagatgc cggatgcggc gtaaacgcct tatccggcct acggctcggt
     2701 ttgtaggcct gataagacgc gccagcgtcg catcaggctc cgggtgccgg atgcagcgtg
     2761 aacgccttat ccggcctacg gctcggattt gtaggcctga taagacgcgc cagcgtcgca
     2821 tcaggcacag gatgcggcgt aaaatgcctt atccggcatt aaactcccaa caggacacac
     2881 tcatggcata taccaccttt tcacagacga aaaatgatca gctcaaagaa ccgatgttct
     2941 ttggtcagcc ggtcaacgtg gctcgctacg atcagcaaaa atatgacatc ttcgaaaagc
     3001 tgatcgaaaa gcagctctct ttcttctggc gtccggaaga agttgacgtc tcccgcgacc
     3061 gtatagatta ccaggcgctg ccggagcacg aaaaacacat ctttatcagc aacctgaaat
     3121 atcagacgct gctggattcc attcagggtc gtagcccgaa cgtggcgcta ttgccgctta
     3181 tttctattcc ggaactggaa acctgggtcg aaacctgggc gttctcagaa acgattcatt
     3241 cccgttccta tactcatatc attcgtaata tcgttaacga tccgtctgtt gtgtttgacg
     3301 atatcgtcac caacgagcag atccagaaac gtgcggaagg gatctccagc tattacgatg
     3361 aactgatcga aatgaccagc tactggcacc ttctgggcga aggcacccac accgttaacg
     3421 gtaaaaccgt aaccgttagc ctgcgcgagc tgaagaaaaa actgtatctc tgcctgatga
     3481 gcgttaacgc gctggaagcg atccgcttct acgtcagctt tgcttgttcc tttgcctttg
     3541 cagaacgcga actgatggaa ggtaacgcca agattattcg cctgattgcc cgcgatgaag
     3601 ccctgcacct gaccggtact cagcatatgc tgaatctgct gcgcagcggc gcggatgatc
     3661 cggagatggc ggaaattgcc gaagaatgta agcaggagtg ctatgacctg tttgtccagg
     3721 cggcacagca agagaaagac tgggcggatt atctgttccg tgacggttcg atgattggtc
     3781 tgaataaaga tattctctgc cagtacgttg aatacatcac caatatccgc atgcaggcag
     3841 tcggtttgga tctgccgttc cagacgcgct ccaacccgat cccgtggatc aacacctggc
     3901 tggtgtctga taacgtgcag gttgctccgc aggaagtgga agtcagttct tatctggtcg
     3961 ggcagattga cgcggaagtg gacaccgacg atttgagtaa cttccagctc tgatggcccg
     4021 cgttaccctg cgcatcactg gcacacaact gctgtgccag gatgaacacc cttcccttct
     4081 ggcggcgctg gaatcccaca atgtggcggt tgagtaccag tgccgcgaag gttactgcgg
     4141 ctcctgtcgc acccgactgg tcgcaggtca ggtggactgg attgccgaac cgttagcctt
     4201 tattcagccg ggggaaattt tgccctgttg ttgccgggca aaaggcgata ttgaaatcga
     4261 gttgtgatcg ggtactagcg ccagatacga cgcttacgcg tattatctgg cctacaacga
     4321 ttacatggcg tagtaatacg ctttcacctg ctcccagtcc gctttaggga tcggctccag
     4381 atatttttcc agctggcgga agtcatgatt aatcgcttta tcgcgacgca agcggcgacg
     4441 acttttctcc agatcaagaa aaccagcttc tgccttacct tctgttttca catagatatg
     4501 gcgaacatag caacagccat gctgacgatt aacgctatgc attttcttaa acgccagtgc
     4561 cactgctttc aacatggctt gccgtacttc gtcagaataa ggcgatactg catgccgggc
     4621 ataccagtca gcaatgctga tgaaccccgc catatcttca gtcaccagca acgcgcgcca
     4681 ttcaccctca attttcaccg cttcaccaaa aacgatcttc ggcacgatga caccggcccg
     4741 ttccagttct ttaattaccg caacctcacg gacaatcgtt ggtcggccga acggataacg
     4801 tactgaatga aacagatgat gcgtcatgcg ctttacatac agctttttgc cgttgcgctc
     4861 gacgcattgc accccgctca taccattacg gcgatagtta ggctcttcaa cccagtcgcc
     4921 ctctgttgcc caccagtgat taaattcgtc gtactttgct gaaactgcca taccctatcg
     4981 cctgtcattt ttattaacga caatgactat aggtggttac ctgaggaaaa tcttaatgaa
     5041 acgtgtcgta ttaatgaatt tcttttgccg ctacaggtgt gacaccgctt ttgcccttca
     5101 tttgcagcgc atagattcca ccagccacta ccactaatag tgcgcccagc agataaaatg
     5161 tgaatttaat cagtcggata cagcgatgaa ttttattcga cagggattag gcatcgcctt
     5221 acaaccagag ttaacgctga aaagcattgc aggtgaattg tgttccgttc ctcacgaacc
     5281 aaccttctat cgacagattt cgttgctgac taaagaaaag ccggtagaag gcagtccact
     5341 gtttttacta caaatgtgca tggaacaatt agtggcgatt ggaaaaattt gatatgagac
     5401 aggatggcgc atgaacgcca tcctgcagta acattactct ttattaagaa attttactgc
     5461 cttatcaggg aaatcagtaa acagcccatt tacacccgct ttgttataca gagcatcata
     5521 taactgattc acatcaggag tgtattcagg cagtttatct gaccgcacgg tataaggatg
     5581 cactaccagt ttattctgct gggcatcttg caccatgcca gtgagtttga tattacccgg
     5641 ctgcgatgtc tcctcaatca acatatggta atccggacca ataccatctg catattccgc
     5701 cacctgtttc atggctcccg gcttaaacat ccagtcgtag ttgtaattaa cccagcttcc
     5761 atccggctgt ttctgctgcg tttcattcca gtcggtatag gcaatcagct gtaccagatt
     5821 gagctccatg cccattttgg gttccagctc attcttaata cgcttcagct catcagcatc
     5881 aaaacattgc aaataaactt tatcgtcttt accggtgtaa ccatatttct tcagcacttc
     5941 cagcgttttt gccgcaatat ccttcccttc ctgatgatgg aaccacggcg ctttgatttc
     6001 tggatagata ccgatatttt tcccggtaga gtgatttaac ccctgaacaa attcaatctc
     6061 ttcttcaaag gtgtgcaccc ggaagtcgga cttacccatt gggaaacgcc ccggataagt
     6121 ctgtactttt ttaccgtttt caatatcgaa accttcggta aatttcagcg acttaatttc
     6181 atccagcgtg aaatctatcg cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg
     6241 atcggcaaca tcagtaacac gatcgagata atggtcatgc agaacaacca gatggtcgtc
     6301 tttggtcatc accaaatcct gttccagata atccgctccc tgcgcatacg ccatcgcttt
     6361 tgctggcagc gtatgctccg gcaaatatcc actggcaccg cgatgggcga tgactatttt
     6421 ttcgttgctg tccgccgcca ttgcactgct tcccatgact atagtgctca tcatgatcgc
     6481 catgctaagg tttttcagct tcaatttcat gccattagcc tccgttgcgt ttttgcagta
     6541 attgttcatg gcgacgtttt tcgccaatca tcacaacaat caacaagata accgccagaa
     6601 tgctgccgcc aatcattacc ataaagccgc catcccagcc gaagaagtcc acggtgtagc
     6661 caacaatcgc gctcgccgcc accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg
     6721 ctgccgtacc tgccgctttt ttcggtgcca gttccagcgc atgcagaccg atcagcatca
     6781 caggaccgta gatcaggaag ccgataacaa tcatacaaat catatcgacg gttgggttac
     6841 ctgccgggtt catccagtaa acgatagtcg cgatggtcac cagtgtcata aagaagacgc
     6901 cggttgcccc acggttacca cggaagactt tgtccgacat ccagccgcac agcagggtgc
     6961 ccggaatacc cgcatactca tagaagaagt aggcccagga ggatttatcc agcgcgaaat
     7021 gcttaacctc tttcagataa gtcggtgacc agtcgaggat gccgtaacgc agcagataaa
     7081 cgaacacgtt ggcgatggcg atgtaccaca gcagtttgtt cggcagcacg tactgcatga
     7141 agatttgctt cgcggtcagc tcctgttccg ctttttcgtt atagtcgtcc ggataatcat
     7201 ttttgtactc ttcgatcggc ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa
     7261 aggcgaataa tgccaccaga atggcgcaga aagcaggcat atagagcgcc gcatgccagt
     7321 cattgaacca ggccatcccc agcaggaaca gcagcggcgg aataccacca ccgacgttgt
     7381 gcgcacagtt ccacactgac acaatgccgc cacgttcttt ctgcgaccac cagtgcacca
     7441 tagtacgacc acacggcggc caccccatcc cctggaacca accgcagagg aacaacagta
     7501 caaacatcac cgcaatgctc gacgtcgccc atggcacaaa gcccataaac aacatcactg
     7561 ccgccgccag aatcaaacct gcgggcagga aaacgcgcgg attcgagcga tccgataccg
     7621 aacccatgat gaatttcgaa aatccataag caatcgagat ccccgaaagg gcaaaaccta
     7681 aatcaccgcg tgagaatccc tgctcaacca gataaggcat agcaagcgca aagttcttac
     7741 gaaccaaata gtaagccgca tagccaaaga atatccccag gaaaatttgc cagcgcaatc
     7801 gacgataagt cggatcgatc tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa
     7861 aaatactcaa cattgatagc ctccgtggcc cgtggtctta tttatgatta acagcctgat
     7921 tcagtgagag aacctgccgt ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt
     7981 aaatgtgaat tgccgcacac attattaaat aagatttaca aaatgttcaa aatgacgcat
     8041 gaaatcacgt tttactttcg aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt
     8101 tttactatgg ctaaatggta aaaaacgaac ttcagaggga taacaatgaa aactcgcgac
     8161 tcgcaatcaa gtgacgtgat tatcattggc ggcggcgcaa cgggagccgg gattgcccgc
     8221 gactgtgccc tgcgcgggct gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt
     8281 gccaccgggc gtaaccacgg cctgctgcac agcggtgcgc gctatgcagt aaccgatgcg
     8341 gaatcggccc gcgaatgcat tagtgaaaac cagatcctga aacgcattgc acgtcactgc
     8401 gttgaaccaa ccaacggcct gtttatcacc ctgccggaag atgacctctc attccaggcc
     8461 acttttattc gcgcctgcga agaagcaggg atcagcgcag aagctataga cccgcagcaa
     8521 gcgcgcatta tcgaacctgc cgttaacccg gcactgattg gcgcggtgaa agttccggat
     8581 ggcaccgttg atccatttcg tctgaccgca gcaaacatgc tggatgccaa agaacacggt
     8641 gccgttatcc ttaccgctca tgaagtcacg gggctgattc gtgaaggcgc gacggtgtgc
     8701 ggtgttcgtg tacgtaacca tctcaccggc gaaactcagg cccttcatgc acctgtcgtg
     8761 gttaatgccg ctgggatctg ggggcaacac attgccgaat atgccgatct gcgcattcgc
     8821 atgttcccgg cgaaaggatc gctgctgatc atggatcacc gcattaacca gcatgtgatc
     8881 aaccgctgcc gtaaaccttc cgacgccgat attctggtgc ctggcgatac catttcgctg
     8941 attggtacca cctctttacg tattgattac aacgagattg acgataatcg agtgacggca
     9001 gaagaggttg atattctgct gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg
     9061 cgcattttgc gggcctattc tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc
     9121 ggacgtaacg tcagccgtgg catcgtgctg ctcgaccatg ctgaacgcga tggtctggac
     9181 ggatttatca ccatcaccgg tggcaaactg atgacctatc ggctgatggc tgaatgggct
     9241 accgacgcgg tatgccgcaa actgggcaac acgcgcccct gtacgactgc cgatctggca
     9301 ctgcctggtt cacaagaacc cgctgaagtt accttgcgta aagtcatctc cctgcctgcc
     9361 ccgctgcgcg gttctgcggt ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa
     9421 ggccgtctac accgtagcct ggtatgtgag tgcgaagcgg taactgcggg tgaagtgcag
     9481 tacgcggtag aaaatttaaa cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg
     9541 gggatgggca cctgccaggg cgaactctgc gcctgccgcg ctgccggact gctgcaacgg
     9601 tttaacgtca cgacgtccgc gcaatctatc gagcaacttt ccaccttcct taacgaacgc
     9661 tggaaaggcg tgcaacccat cgcctgggga gacgcactgc gcgaaagcga atttacccgc
     9721 tgggtttatc agggactgtg tggtctggag aaggagcaga aagatgcgct ttgatactgt
     9781 cattatgggc ggcggcctcg ccggattact ctgtggcctg caactgcaaa aacacggcct
     9841 gcgctgtgcc attgtcacgc gtggtcaaag cgcgttgcat ttctcatccg gttcgctgga
     9901 tttgctgagc catctgccag atggtcaacc cgtggcagac attcacagtg gactggaatc
     9961 tttgcgtcag caggcacctg cacatcctta ctctcttctc gggccacaac gcgtgctcga
    10021 tctcgcttgc caggcgcagg cattaatcgc tgaaagcggt gcgcaattgc agggcagcgt
    10081 agaactggct caccagcgga ttacgccgct cggcactttg cgtgcaacct ggttaagttc
    10141 gccagaagtt cccgtctggc cgctacccgc gaagaagata tgtgtagtgg ggataagcgg
    10201 gctgatggat ttccaggcgc atcttgcggc ggcttcgttg cgcgaactgg atctttcggt
    10261 tgaaaccgca gaaatagagc tgccggaact ggatgtcctg cgcaataacg ccaccgaatt
    10321 tcgcgcggtg aatatcgccc gtttccttga taatgaagaa aactggccgc tgttacttga
    10381 tgcgcttatt cctgtcgcca atacctgcga aatgatcctg atgcccgcct gcttcggcct
    10441 ggccgatgac aaactgtggc gttggttgaa tgaaaaacta ccttgttcac tgatgctttt
    10501 gccaacgctg ccgccttccg tgctgggcat tcgtctgcaa aaccagttac agcgccagtt
    10561 tgtgcgccaa ggtggagtgt ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa
    10621 tggcgtagtg aatgaaatct ggacccgcaa tcacgccgat attccgctac gtccacgttt
    10681 cgcggttctc gccagcggca gtttctttag tggcggacta gtagcggaac gtaacggcat
    10741 tcgcgagccg attctcggcc ttgatgtgct acaaaccgcc acgcggggtg aatggtataa
    10801 gggcgatttt tttgcgccgc aaccgtggca gcagttcggt gtaaccactg atgagacgct
    10861 acgcccgtcg caggcagggc aaaccattga gaacctgttt gccatcggtt cggtgctggg
    10921 cggatttgat cccatcgccc agggatgcgg cggcggtgtt tgtgccgtca gtgctttaca
    10981 tgccgctcaa cagattgccc aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa
    11041 aactgcatta agtgcaccgt ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt
    11101 tatccagggc caaaacaagc cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca
    11161 ctgtatgacg aggcgctgaa atattgcatc aactgcaaac gttgtgaagt cgcctgcccg
    11221 tccgatgtga agattggcga tattatccag cgcgcgcggg cgaaatatga caccacgcgc
    11281 ccgtcgctgc gtaattttgt gttgagtcat accgacctga tgggtagcgt ttccacgccg
    11341 ttcgcaccaa tcgtcaacac cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg
    11401 gcgttaaaaa tcgatcatcg tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc
    11461 tggtatcgca gcatagcggc tcagcaagca caatataaag accaggtcgc tttctttcac
    11521 ggctgcttcg ttaactacaa ccatccgcag ttaggtaaag atttaattaa agtgctcaac
    11581 gcaatgggta ccggtgtaca actgctcagc aaagaaaaat gctgcggcgt accgctaatc
    11641 gccaacggct ttaccgataa agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc
    11701 gaagctgtgg gagtaaaagg cattccggtg attgccacct cctcaacctg tacatttgcc
    11761 ctgcgcgacg aatacccgga agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc
    11821 gaactggcaa cccgctggct gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa
    11881 ccgctgccgc tgaaagtggt ttatcacact ccgtgccata tggaaaaaat gggctggacg
    11941 ctctacaccc tggagctgtt gcgtaacatc ccggggcttg agttaacggt gctggattcc
    12001 cagtgctgcg gtattgcggg tacttacggt ttcaaaaaag agaactaccc cacctcacaa
    12061 gccatcggcg caccactgtt ccgccagata gaagaaagcg gcgcagatct ggtggtcacc
    12121 gactgcgaaa cctgtaaatg gcagattgag atgtccacaa gtcttcgctg cgaacatccg
    12181 attacgctac tggcccaggc gctggcttaa actcccttct gatgcccggt aagcatgtgg
    12241 ttaccgggca tttttgcgta cacgattccg tgcccaatgt atgcgttgca acgcagtgaa
    12301 aattcctctg aaaacgtctc gcaaaggctg aaactggcag atgtcaaagg cctgggataa
    12361 ccgtaatgtc gcgtcatcat aaatatcagg tgacggacaa ccatgaccga atcaacaacc
    12421 tcctccccgc atgatgcggt atttaaaacc tttatattca cacccgaaac cgcacgggat
    12481 tttctcgaaa tacatttacc agaaccactg cgcaagcttt gcaacctgca aaccttacgc
    12541 ctggaaccca ctagttttat tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg
    12601 tccgtggaaa ccagcgacgg tgacggctat atctactgcg tgattgaaca tcaaagctct
    12661 gcagaaaaga atatggcttt tcggccaatg cgctatgcca ctgccgccat gcagagtcac
    12721 ctagataaag gctatgacag agttccgctg gtggtgccat tgctgtttta tcatggcgaa
    12781 acatcgccct acccgtactc acttaactgg ctggatgagt ttgacgatcc gcaacttgcc
    12841 cggcagttgt acaccgaagc ttttccgttg gtggatatca ccatcgtacc tgacgatgag
    12901 atcatgcaac atcggcgtat agctctgctg gaactgattc aaaagcatat tcgcgaccac
    12961 gatttaatcg gcatggtcga caggatcacc acgcttttgg ttagaggctt cactaatgac
    13021 agccagctac aaacactgtt taattatctg ctgcaatgcg gcgatacctc ccgtttcacc
    13081 cgttttattc aggagattgc cgaacgttca ccactacaaa aggagagatt aatgactatt
    13141 gctgaacggc tacggcagga aggacatcaa attggctggc aagaaggtaa attagtaggt
    13201 ttgcagcaag gtaaattaga aggtttgcag gaaggtatgc atgaacaagc cattaaaatt
    13261 gctttgcgca tgctggaaca gggcattgat cgtgaccaag tgctcgcggc cacccagcta
    13321 agcgaagccg atctggcagc gaataaccac taattaacac aggcccacag ccgatcccca
    13381 tgggcctttg atatcaataa ctacctttta tgcgtggccc atttttgcct gatttaaaca
    13441 tcgccagtcg ttgatccagg gcatcgctgt agagcatcgt gtcaacgcca acagcgacaa
    13501 agttcgctcc ccacgccagg cattgctgcg ccatatcagg agccacagcc agaaaaccag
    13561 ccgctttacc cgcagcacgg atccgccgaa tgctggtttc aataattcgc tgcacttccg
    13621 ggtgcccggc gttatccggg tagcccaacg acgcagaaag atccgctggt ccaataaaca
    13681 cgccatcaat cccttcgacg tcgaggatct cgtccaggtt atccagtgcc gttttacttt
    13741 ccacctgcac taacagacaa agcgaatcgt tgacttgcgc catgtaattc tcaatgcgtc
    13801 cccagcgcgc agcccgtgcc acactggccc caacaccacg ctcaccgtag ggagggttgg
    13861 acaaattttg ccggaggggg aatgtgccgg tgcagtaaac cggcacagag tggcgaaatc
    13921 agacggataa agtcatggac tcaacaacct caatccagcc atgctcacta gcgatgtcct
    13981 ggccgtttaa ccaacggcgt agcatattca gtgccatcat tgcgcacact tcctgacgga
    14041 tagccaggct gtagcgtgta gtgctgaaac gcacacgcag agcgaaagtg ccgtctggcg
    14101 tggctagcgc aaagttgaga tgctcgttct cgaaacccga aacagccagt gccagcccgg
    14161 caaaatggtt ggcccgccgt tctgtaatcc agtgcgcagt ttgcgccagg gtttcttcct
    14221 gtgaaggaat cacttcacac gccagcaatg gagcacctgc gcgagaaagt tgcaaagcca
    14281 ataaaccacc ggtgaattgc tcgctcaacg tcaggctgaa ctggcggttt tgcaattcgc
    14341 gactgatctg cgcgggcagt ccttcggtgc cttcgaaaat cacgctctgt ccggcaacac
    14401 gtttaacgtc cagccacagt ttttccatcg cctgttgctc gcttgccggt ccggtgagtt
    14461 tcagttcgat gatcggcatt gaggagcgat agcccattgt tacgcccggc ggcagttgta
    14521 gagtgtccag gctttgtgcc agatcgcttt ccgaacgacc aaaggtagtt aaacgcagac
    14581 aaaccggcgg ctgtggtaaa gaaaaacgct cgcgcaggcg cggcaggatt tcgtgctcga
    14641 ccatcacctt aaattctgac ggtacgccgg gagtaaagaa catcaggcaa cgattaagct
    14701 gcacggcaaa accacaggcg gtgcctaccg ggttattgat aaattcagca ctggcaggca
    14761 gctccgcttg tttacggttg ctcggtgcca ttactcgtcc acgttcgtgg aaatagcgtt
    14821 ccatctcttt gagccaggct tcatgcagca ccaggccttc accttttgct gtcgcagcgg
    14881 cgagtgcgct taaatcatcg ctggtcggtc ccagcccgcc gttaacgatc agcacatcgg
    14941 cgtgctgact acgttcgcga agaatggtga ctaagtcatc aaggttatcc cccaccgtat
    15001 tgcggcgaga taatggcagc ccctgatgaa agaaaaaatc ggccagccag gcagcgttag
    15061 tgtcaacgat ttgcccgtat aacacttcat ccccggtgga tagcatttcc acttttaaca
    15121 tggttatctc ctgcttttgt tatcgaatca ctataacgca aagggagata aggcgtagta
    15181 agaaactgaa tgccgcgccg gaagggatca gaaactggcg ttaaccccaa catacaggcc
    15241 gtcagccaca gcgttgtcgc ggttaccgtc tttacccgac agattcaggt agcgataacc
    15301 cgcctcaata ctgactggac gcataatggt gtaacgcgcg ccagcattcg cttcctcata
    15361 actttgaata ccgctggaga gcgaatccgg agagtagtaa tactcgccaa acaaacggaa
    15421 gctgttgcca attttccact gcaaaccacc tcctaccgct gcggcataac cttcatcgcc
    15481 gtaattcggg ttggtgtaca cgccttttcc gccaacggtc gccattaacg gcccgagagg
    15541 cagattcaac cccagcccca cgcccgcgac gtcaccgtcg tcgtcgttat gtgtccagtt
    15601 accgcttaaa gccaggcccg tcgattcagt cccaaaaccg acaccaatat tggtgtattc
    15661 tttacccgcc tggccgctga tgctcattgc attgaccgat gcagaaacca acagcattcc
    15721 ggccagacct gctagcgcaa tttttttcat tatccccttt cccacaagca attaatcaaa
    15781 attcccaaag ctgcgggatt gtactgcctg gcacagagga ttcaatgagc gaaaaaaggg
    15841 atctagtcac ttattgtaac taaatacttc ttaaataatt aaacgccgaa aaaataaaca
    15901 cactttaccg atacacacaa aaataataat aagtgcagtt atcattgcaa aagagattac
    15961 tgtcactttc acatatttct gagttaccgt attctcatgg tttcttttta tttataagga
    16021 atacccctgt gcgacaacgg actattgtat gccctttgat tcaaaatgat ggtgcttatt
    16081 tgctgtgcaa aatggccgac gatcgcggcg ttttccccgg tcaatgggcg ctttcgggtg
    16141 gcggcgtgga gtctggcgaa cgaattgaag aggcactacg ccgcgaaatt cgcgaagaac
    16201 tgggagaaca gctgcttttg acagaaatca cgccgtggac cttcagcgat gatattcgca
    16261 ccaagacata tgcagatggt cgcaaggaag agatttatat gatttacctg atttttgact
    16321 gcgtttctgc caaccgagaa gtgaaaataa acgaagagtt tcaggactac gcgtgggtaa
    16381 aacctgaaga tctggtgcat tatgatttaa atgttgccac ccgaaaaacg ttacgtttga
    16441 aaggtcttct ttaacaatga tgacagccat cactggctgt cagattgaaa atttagtggt
    16501 taacgaattc cccatccaga taaactttgc ctttttccac atgcatgact aaaccatcca
    16561 gataatcagg tttaaatatc gcatcacgct tatttttagc aatatatgtc aggcaatgat
    16621 tatgggtgaa aataacgata tttttatcag gcgctttgct ttgtaagtcc ttaattgcac
    16681 tataaatctc attaccgcac tgaagaagtc gtttatctac cgtcagcttt ttacccgcgg
    16741 aaaaccaggt cgccgactga atggtccgga cggtattact ggaataaaga tcgaaatcag
    16801 agatgtcagc gctaaaagcg ttgcccagtt cacgggcatc ctgggtacct ttaaccgtaa
    16861 tacctgtttt atctgacaag cattgattgg ttgaacgatc gcaacgttca gcatggcgaa
    16921 acaaaacgac aaccgggtgc tgctgtgcca gtctggccaa tgttttattg tcgatacgtg
    16981 gcaaaccatt gctactccag gcggcatgag tacccagtcc ggcaattgca gcgagcgcca
    17041 gtaaaatgat gaaatatttt tttgacttca atgatgagcg gcaaaaagct aacacgtaaa
    17101 ctccacctat agacaagcgc aaccagacaa ttaccgtgaa attgagcgac atttctggcg
    17161 ataattagca gttggtgtaa tattaaaaat cctacgatgt cggcaaaatg cctcaaattt
    17221 ttgccaaatg caaagcctaa ataagaaaaa atataaaaat ttcaatattt acgtctaata
    17281 ttagtttctt aaggttaagt taatattcta tccttaaaat ttcgctccaa atggcaaaat
    17341 atacacaaca ctctttatag caaatataag tggacaggta ttcaatggcg gaaggaaaag
    17401 caatgtcaga atttttgcct ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag
    17461 ttaaagaggt tctcgaatcc ggttggatca caaccggtcc gaagaatcag gcgcttgagc
    17521 aagccttttg ccagttgacg ggaaatcagc atgccatcgc ggtcagttca gccaccgccg
    17581 gaatgcatat cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg attacgcctt
    17641 ccctgacctg ggtttcaacc ctcaatatga tttccttgct gggtgcaacg ccggtaatgg
    17701 tggatgtcga ccgcgatacg ctgatggtca cgcctgaagc gatcgaatca gccattacgc
    17761 cacgcactaa agccatcatt ccggtgcatt atgccggtgc gccagcagat attgacgcca
    17821 ttcgcgccat tggcgaacgt tacggcatcg cagttatcga agatgctgcc catgccgtcg
    17881 gtacgtatta caaagggcga catattggcg caaaaggtac cgctattttt tcatttcatg
    17941 ccattaaaaa tattacctgt gctgaaggtg gcctgattgt aactgataat gaaaaccttg
    18001 cccgccagct acggatgctg aaatttcacg gtctgggtgt cgatgcctat gacagacaaa
    18061 cctggggccg tgcaccgcag gctgaagtct taacaccggg ctataagtac aatctgaccg
    18121 atattaacgc cgcgattgcc ctgacacagt tagtcaaatt agagcacctc aacacccgtc
    18181 ggcgcgaaat tgcccagcaa tatcagcaag cactggcagc tctccccttt cagccattaa
    18241 gccttcccgc ctggccgcac gttcacgcct ggcatctgtt tattattcgt gtcgatgaac
    18301 aacgttgtgg tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga ggcattggta
    18361 ccgggttaca tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt tttcccacgc
    18421 tgtcgttacc gaataccgaa tggaatagcg aacgcatctg ttcgttgccg ctgttcccgg
    18481 atatgactac cgccgatgcc gaccgcgtca tcacagccct tcagcaactc gcaggacaat
    18541 aagccatgtt tgaaatccac cctgttaaga aagtctcggt ggttattccc gtttataacg
    18601 agcaggaaag cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga
    18661 aagagtatga gatcctgctg attgatgacg gcagtagcga taattccgcg catatgctgg
    18721 tcgaagcctc acaagcggag aacagccata ttgtgtctat tttgattaac cgcaattacg
    18781 ggcaacattc agcgattatg gcgggattca gtcacgttac tggcgactta attattaccc
    18841 ttgatgccga tctccagaat ccgccagaag aaatcccccg cctggtggca aaagccgatg
    18901 aaggttacga cgtggtaggg actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa
    18961 cagcttcgaa gatgattaac cggcttattc agcgcaccac tggcaaagcg atgggtgact
    19021 acggttgtat gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg cactgccatg
    19081 aacgcagcac ctttatcccg attctggcga atatcttcgc ccgccgtgca attgaaattc
    19141 cagtacatca tgccgagcgt gagtttggtg aatccaaata cagttttatg cgcctgatta
    19201 atttgatgta cgacctggtg acctgcctta ccacaacgcc gctacgtatg ctgagtctgc
    19261 tcggcagcat tattgcgatt ggaggtttta gcattgcggt gctgctggtg attttacgcc
    19321 tgaccttcgg accacaatgg gcggcagaag gcgtctttat gctatttgcc gtgctgttta
    19381 cttttattgg cgctcagttt atcggcatgg gattactcgg tgaatatatc ggcaggatct
    19441 acaccgatgt ccgcgcccgc ccccgctatt ttgttcagca agttatccgt ccatccagca
    19501 aggaaaatga ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat gcctcggtat
    19561 tgaagctctg ctggctgccg gttacgaaat tagcgccatt tttacccata ccgataatcc
    19621 cggtgaaaaa gccttttatg gttcggtggc tcatctggcg gcggaaagag acattccggt
    19681 ttatgcgccg gataacgtta atcatccgct gtgggtggaa cgcattgccc aactgtcacc
    19741 agaggtgatt ttctcttttt attatcgcca tcttatttgc gacgagattt ttcagctcgc
    19801 tcccgcaggt gcatttaatc tgcacggttc gctgttacca aaatatcgtg gtcgcgcgcc
    19861 gctgaactgg gtgctggtga acggtgaaac ggaaacgggc gttacattgc accgaatggt
    19921 aaacgtgccg atgctggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat
    19981 atcgctatta cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca
    20041 ttacccgcca ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa cgaagccacc
    20101 tgttttggtc gcagaacgcc ggatgacagt ttccttgaat ggcataaacc ggcatccgta
    20161 ctgcacaaca tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc
    20221 aatcagaaat tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg
    20281 gggagcgtga tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc
    20341 gtcaccggac aggcgggcga cggcattact atgcagggct cgcaattagc gcagatgctg
    20401 ggcctggtgc aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc
    20461 cgggtactca tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg
    20521 cgcgaagatc attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt
    20581 ctgaatcatc cacattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt
    20641 gagtatcacg tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt
    20701 gaatataccc gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt
    20761 atccgctact gcgttaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat
    20821 gggatgtgta gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg
    20881 aataaaccac gctggattta ttcggtgtca aaacaattac ttgatcgggt gatctgggcc
    20941 tatggcgaaa aagagggttt acagttcacc ctcttcctcc cgtttaactg gatgggacca
    21001 cgactggata accttaatgc agcgcgaatt ggcagctccc gcgctattac gcaactcatt
    21061 ctcaatctgg tagaaggttc accaattaag ctaattgatg gcggaaaaca aaaacgctgc
    21121 tttactgata tccgggacgg tatcgaggcg ttgtaccaca ttatcgaaaa tgcgggaaat
    21181 cgctgcgacg gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa
    21241 ctgggcgaga tgctgctggc gagcttcgaa aaacatccgc tgcgccatca tttcccaccg
    21301 tttgcgggct ttcgcgttgt cgaaagtagc tgctactacg gcaaaggata tcaggacgta
    21361 gagcatcgta aaccgagcat ccgcaatgcc caccgctgcc tggactggga gccgaaaatt
    21421 gatatgcagg aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg
    21481 gataaaccat catgaccaaa gtaggcttac gcattgatgt cgatgccttt cgtggcaccc
    21541 gtgaaggcgt gccgcgtctg ctggaaatct tgagtaagca taatattcag gccagcattt
    21601 ttttcagcgt cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt
    21661 ttttgtggaa gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg
    21721 caggtacggc ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag
    21781 cggctaaaca tcacgaagtc ggtctgcacg cctgggatca ccatgcctgg caagcccgta
    21841 gcggtaactg ggatcggcaa acaatgatcg acgatattgc acgcggtctt cgcactctgg
    21901 aagagattat cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccaga
    21961 aggtgatcga agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca
    22021 taccgttccg tccactgctt gaatcaggaa accctggcac tgcgcaaatt ccggtgacct
    22081 tacccacctg ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt
    22141 tactcaaccg catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg
    22201 aaggctgcgc ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg
    22261 tgacattttg ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc
    22321 gcggaaatat tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct
    22381 gatgaaatcg gtacgttacc ttatcggcat cttcgcgttt attgcctgct attacctgtt
    22441 accgatcagc acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg aaatcagtcg
    22501 agaaatgctg gcatccggcg actggattgt gccccatctg ttagggctac gttatttcga
    22561 aaaacccatt gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa
    22621 ctttggtgtg cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg
    22681 gtttactctg cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct
    22741 ctcattgttt attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt
    22801 ctggctggtg gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg
    22861 caaaagcgca ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg
    22921 ttttctcgcc cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg
    22981 ctggaaagat ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct
    23041 cccctgggga ctggcgatag cgcagcggga gcctgacttc tggcactatt ttttctgggt
    23101 tgagcatatt caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta
    23161 cgtgccggtc atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta
    23221 cacaggctgg aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat
    23281 gccgctgctg tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt
    23341 tgcacctctg gcaatgctgt tggcgcatta cgcgttgctg gcagcaaaaa ataatcctct
    23401 ggcgctgcgg attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac
    23461 atttgtgatc tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta
    23521 taaagtcttt tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt
    23581 aacaaacgtc gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct
    23641 ggtaggattt tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga
    23701 gatgacacaa gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc
    23761 cgcaggtctg gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga
    23821 gttgaaatac ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt
    23881 cgcaaactgg cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga
    23941 ccgcgatgaa gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga
    24001 gcgtctggtg ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc
    24061 ttgcttagcg ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac
    24121 aaacggcgca aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc
    24181 atgatgctct ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta
    24241 agtctgaatt ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg
    24301 ccgcgtcact ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg
    24361 gtgtaatgtg cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa
    24421 gcctgggttt tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg
    24481 ctctgttagc ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc
    24541 tttccgtttt ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct
    24601 tgctcagtat gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag
    24661 ggactttttc gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga
    24721 ttttcctgcc cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt
    24781 cccactcatc ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac
    24841 gattaataca ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga
    24901 agtcactctt cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta
    24961 catgtctgtt atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta
    25021 tcctgtttgc taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac
    25081 ggcaatggaa tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac
    25141 ccactctttt agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa
    25201 agtcagccag cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag
    25261 atcaacggtt tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc
    25321 ggcaacggaa acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac
    25381 ttcttccggc tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat
    25441 ggtcagcttg ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt
    25501 aaccagtgaa accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag
    25561 ccacacttca ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc
    25621 caggccgtcg gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca
    25681 aaagcaacga atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc
    25741 gccaagtaac accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg
    25801 tgttggcacc agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc
    25861 acgcaccgtc atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac
    25921 gtgaaacagc ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa
    25981 cacaccttgc gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc
    26041 ggtagaaccc gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg
    26101 tacgccttca accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc
    26161 cggcaccagc gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg
    26221 cagctgaggg ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag
    26281 cagcgtttgc ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccaccccctg
    26341 cgccgcaaag cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg
    26401 ctcgtcatta agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg
    26461 ccagtcagag aagatcataa caactgctcc tgtacatcag cgtccactaa cggcagtggg
    26521 ctccccggcc agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga
    26581 atggtgtctg gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac
    26641 tcaatggaag aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg
    26701 cgtacttttt ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct
    26761 tcagccacaa aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg
    26821 gtttcacggg caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg
    26881 atgcggtggc gataatccgg gttaacatat ttggcaaact gctgaccttt cagcggtgtc
    26941 caggcgcgat ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc
    27001 accaccatgc cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg
    27061 cccggcatat cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc
    27121 gcacggtagt tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg
    27181 ccaaaggcca cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc
    27241 caggcaagca gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg
    27301 gagctctccc cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg
    27361 gtttttaacc gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat
    27421 acctgcgcgc tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct
    27481 ggttgaaagc gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt
    27541 tgcccgccag ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca
    27601 ggcagcgcag cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt
    27661 tttccagatc cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg
    27721 cgtcgtactg acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag
    27781 ccatgtagga agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga
    27841 agatggcatt atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc
    27901 cgatggagta gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct
    27961 ggaagtccag cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac
    28021 cacgcacttt ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga
    28081 tcacgccgat attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg
    28141 tcagaggacg gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt
    28201 cggtggattt ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg
    28261 gtgcgtaagg cattgcttca tcaggataaa tcatattgtg tcctttagtc agaaacgcaa
    28321 gatctgcgcc agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc
    28381 gcgaggaatg acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc
    28441 acgttcacca cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc
    28501 aggctggacg gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt
    28561 gctgcgcagc gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg
    28621 ttgataccag tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg
    28681 gcgatcggaa cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc
    28741 ttcgacaata accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg
    28801 tccgccaagc gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt
    28861 acgcagtaag tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg
    28921 acctaggaga tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca
    28981 ttcgtggcaa tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctagttttcc
    29041 gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc
    29101 gtttgcgcac catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc
    29161 caggcgtcgg caaatgccgt ttcaagttcc tgccagtttt gcggatgatg atatttcagc
    29221 tcgaacatcg cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca
    29281 cgctcgcttt gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca
    29341 attaatacca gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagc
    29401 gcggagagat cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg
    29461 gcggtcgaaa gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct
    29521 gccggaagtt gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat
    29581 tgcccctgtt caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca
    29641 tcacggcggg caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac
    29701 cagggctggc gtttttctgc cggatgcagc tccagccagt cggcaatatt ggcaattaag
    29761 cgacgtccgc gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac
    29821 tcttctggtt cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctacttccc
    29881 agttgcacca caatttgcgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa
    29941 agatcggcac acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag
    30001 ccaagagttt gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca
    30061 accaccacgc cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga
    30121 cgaggcgctt cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc
    30181 cagctaagcc cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata
    30241 tggactcccc ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt
    30301 gcggggatat cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac
    30361 attcccggct ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg
    30421 gcggttaaga gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga
    30481 tttgccaccg ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc
    30541 agccccagcg ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg
    30601 gaattctccg ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg
    30661 tgtctgacgc cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat
    30721 gcgcttactg acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa
    30781 aaatagatta ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa
    30841 agtacgcagc cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc
    30901 acggacaatg cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg
    30961 cagggaaacg cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca
    31021 ttctcgggtg aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc
    31081 agcaactgct gccgttggca gcaactgatg taaacagatc acatcatccg ctttgttgag
    31141 tgaagtccag atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg
    31201 taaaacatcc agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag
    31261 catgttctcg cgctggtttt tatcatccgc catcagccac tctcctaact gctgcgcctg
    31321 cttatcatca ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc
    31381 acgccgccgc cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc
    31441 catgtaaaaa tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc
    31501 gttgaccgga cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc
    31561 accttcggcg atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg
    31621 ccagtgttgt tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag
    31681 tgtggcgata aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag
    31741 cgtcagccgc agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa
    31801 attgccctgc gacgggtcaa aagcattcag cccccaaatg cgtaagtcag cgtgttccgg
    31861 gtgctggcga agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag
    31921 gacggcagct tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg
    31981 actggccagc cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac
    32041 ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc
    32101 cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac
    32161 tacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc
    32221 tagtaccagc ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta
    32281 gtcatcagca cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct
    32341 aacccgtttt ttcacatcat ccagcgcttt ttctgcatgc gctttcagct caacatattt
    32401 ctgatcggcg ggatcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt
    32461 caggtcgtca tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg
    32521 taaaacctgt cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc
    32581 catcccaatg tgtgggatac catcttcttc atagacctcc gtcacaggga taaaaccgaa
    32641 actctggtag aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca
    32701 gtgatgcgta catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg
    32761 caacgcttca ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt
    32821 cagaatcctc gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc
    32881 ccccgtcaaa tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac
    32941 cgcgcaacgc agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag
    33001 atcttgccat tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata
    33061 ttttttgaaa cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc
    33121 aacccgcacg cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg
    33181 actttggttg tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa
    33241 ccctggaaaa ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt
    33301 acccggcttg ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg
    33361 tccgcaaggt atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga
    33421 ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa
    33481 agtaaccgct tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca
    33541 tgataaaccg ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc
    33601 actgtttcag gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa
    33661 cggcgcagat tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac
    33721 cggcccctgc gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc
    33781 gacgctggat ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca
    33841 ggctgcgaca ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc
    33901 gcgctatgat gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc
    33961 gactgaactg gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac
    34021 agggtgaaac ttgccctgtt atagcaaccc
//
LOCUS       DAJTYL010000033        33961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYL010000033 DAJTYL010000000
VERSION     DAJTYL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.033"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            197..1147
                     /gene="corA"
                     /locus_tag="OO572_001533"
     CDS             197..1147
                     /gene="corA"
                     /locus_tag="OO572_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6660578.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1188..1568)
                     /locus_tag="OO572_001534"
     CDS             complement(1188..1568)
                     /locus_tag="OO572_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6660579.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(1582..1998)
                     /locus_tag="OO572_001535"
     CDS             complement(1582..1998)
                     /locus_tag="OO572_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660580.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2057..2947)
                     /gene="rarD"
                     /locus_tag="OO572_001536"
     CDS             complement(2057..2947)
                     /gene="rarD"
                     /locus_tag="OO572_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6660581.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(2999..3466)
                     /locus_tag="OO572_001537"
     CDS             complement(2999..3466)
                     /locus_tag="OO572_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6660582.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3631..4500
                     /gene="pldA"
                     /locus_tag="OO572_001538"
     CDS             3631..4500
                     /gene="pldA"
                     /locus_tag="OO572_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6660583.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4633..6462
                     /gene="recQ"
                     /locus_tag="OO572_001539"
     CDS             4633..6462
                     /gene="recQ"
                     /locus_tag="OO572_001539"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6660584.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            6526..7146
                     /gene="rhtC"
                     /locus_tag="OO572_001540"
     CDS             6526..7146
                     /gene="rhtC"
                     /locus_tag="OO572_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6660585.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7208..7828)
                     /gene="rhtB"
                     /locus_tag="OO572_001541"
     CDS             complement(7208..7828)
                     /gene="rhtB"
                     /locus_tag="OO572_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6660586.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7939..8961
                     /gene="pldB"
                     /locus_tag="OO572_001542"
     CDS             7939..8961
                     /gene="pldB"
                     /locus_tag="OO572_001542"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6660587.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8969..9769
                     /gene="yigL"
                     /locus_tag="OO572_001543"
     CDS             8969..9769
                     /gene="yigL"
                     /locus_tag="OO572_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6660588.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9845..10744
                     /gene="bioP"
                     /locus_tag="OO572_001544"
     CDS             9845..10744
                     /gene="bioP"
                     /locus_tag="OO572_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6660589.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10632..11585)
                     /gene="metR"
                     /locus_tag="OO572_001545"
     CDS             complement(10632..11585)
                     /gene="metR"
                     /locus_tag="OO572_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6660590.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11821..14082
                     /gene="metE"
                     /locus_tag="OO572_001546"
     CDS             11821..14082
                     /gene="metE"
                     /locus_tag="OO572_001546"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6660591.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14121..14936)
                     /locus_tag="OO572_001547"
     CDS             complement(14121..14936)
                     /locus_tag="OO572_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6660592.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15198..15959
                     /gene="udp"
                     /locus_tag="OO572_001548"
     CDS             15198..15959
                     /gene="udp"
                     /locus_tag="OO572_001548"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6660593.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16100..17527
                     /gene="rmuC"
                     /locus_tag="OO572_001549"
     CDS             16100..17527
                     /gene="rmuC"
                     /locus_tag="OO572_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6660594.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17622..18377
                     /gene="ubiE"
                     /locus_tag="OO572_001550"
     CDS             17622..18377
                     /gene="ubiE"
                     /locus_tag="OO572_001550"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6660595.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18391..18996
                     /gene="ubiJ"
                     /locus_tag="OO572_001551"
     CDS             18391..18996
                     /gene="ubiJ"
                     /locus_tag="OO572_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6660596.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18993..20633
                     /gene="ubiB"
                     /locus_tag="OO572_001552"
     CDS             18993..20633
                     /gene="ubiB"
                     /locus_tag="OO572_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6660597.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20712..20981
                     /gene="tatA"
                     /locus_tag="OO572_001553"
     CDS             20712..20981
                     /gene="tatA"
                     /locus_tag="OO572_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6660598.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20985..21500
                     /gene="tatB"
                     /locus_tag="OO572_001554"
     CDS             20985..21500
                     /gene="tatB"
                     /locus_tag="OO572_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6660599.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21503..22300
                     /gene="tatC"
                     /locus_tag="OO572_001555"
     CDS             21503..22300
                     /gene="tatC"
                     /locus_tag="OO572_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6660600.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKAEAESEKTEE"
     gene            22342..23124
                     /gene="tatD"
                     /locus_tag="OO572_001556"
     CDS             22342..23124
                     /gene="tatD"
                     /locus_tag="OO572_001556"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6660601.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23121..23609)
                     /gene="rfaH"
                     /locus_tag="OO572_001557"
     CDS             complement(23121..23609)
                     /gene="rfaH"
                     /locus_tag="OO572_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6660602.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23776..25269
                     /gene="ubiD"
                     /locus_tag="OO572_001558"
     CDS             23776..25269
                     /gene="ubiD"
                     /locus_tag="OO572_001558"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6660603.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25315..26016
                     /gene="fre"
                     /locus_tag="OO572_001559"
     CDS             25315..26016
                     /gene="fre"
                     /locus_tag="OO572_001559"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6660604.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26208..27371)
                     /gene="fadA"
                     /locus_tag="OO572_001560"
     CDS             complement(26208..27371)
                     /gene="fadA"
                     /locus_tag="OO572_001560"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6660605.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27381..29570)
                     /gene="fadB"
                     /locus_tag="OO572_001561"
     CDS             complement(27381..29570)
                     /gene="fadB"
                     /locus_tag="OO572_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6660606.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29760..31091
                     /gene="pepQ"
                     /locus_tag="OO572_001562"
     CDS             29760..31091
                     /gene="pepQ"
                     /locus_tag="OO572_001562"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6660607.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31091..31705
                     /locus_tag="OO572_001563"
     CDS             31091..31705
                     /locus_tag="OO572_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6660608.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31744..33195
                     /gene="trkH"
                     /locus_tag="OO572_001564"
     CDS             31744..33195
                     /gene="trkH"
                     /locus_tag="OO572_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6660609.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33207..33752
                     /gene="hemG"
                     /locus_tag="OO572_001565"
     CDS             33207..33752
                     /gene="hemG"
                     /locus_tag="OO572_001565"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6660610.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7998 a         8576 c         9186 g         8201 t
ORIGIN      
        1 agtagatact ggctcactat cctgtcatcc aggatcaact cctaaggcta tccctttttg
       61 ctgatagcct tagcggttgt cagcgacctc aatttttccc gtcgcgctga gtcaggctgt
      121 ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg aactgtccga tattttaagc
      181 attgggagtc ccggtcatgc tgagcgcatt tcaactggaa aataaccgac tgacccggct
      241 ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg attgatcttg tcgaaccgga
      301 cgacgacgag cgactgcgcg tacaatctga acttggccag agcctggcaa cccgcccgga
      361 actggaagac atcgaagcat cggcacgttt ctttgaagac gacgacggcc tgcatattca
      421 ctccttcttc ttctttgaag atgcggaaga tcacgccggt aactccactg tggcatttac
      481 catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa ctgcccgctt ttcgtctgta
      541 tcgtatgcgt gcccgtagcc agtcgatggt agacggtaac gcctacgagt tgctgctgga
      601 tctgttcgaa accaaaatcg aacagttggc agatgaaatt gaaaatatct atagcgacct
      661 ggagcagttg agccgggtga ttatggaagg gcatcagggc gatgagtacg acgaggcgct
      721 ctccacgctg gcggaactgg aagatatcgg ctggaaagtt cgcctgtgtc tgatggatac
      781 ccagcgcgct ctcaacttcc tggtgcgtaa agcgcgttta ccgggtgggc aactggagca
      841 ggcgcgtgaa atcctgcgcg atatcgaatc cctgctgccg cataacgaat ccctgttcca
      901 gaaggtgaac ttcctgatgc aggcggcaat gggttttatc aacatcgagc agaaccgcat
      961 catcaaaatt ttctcggtgg tatccgtcgt gttcctgccg ccaacgctgg ttgcttccag
     1021 ctacgggatg aactttgagt ttatgccaga actgaagtgg agtttcggct accctggcgc
     1081 gattatcttt atgatcctcg cgggcctggc accgtatctg tactttaagc ggaagaactg
     1141 gttgtaaaaa gaaggcggtg gcttagcctg gctaagccac ctgttattca aaggctccag
     1201 gtatttaacc cttttacctc tttctcataa aaccatttgt tcgtgttaac agcaacatag
     1261 gctgctacgg caattcccag aatgttaacg ccaattaatg caccaacaaa tagagataga
     1321 atgccaagta atagaactat aattgctttt ttccataagc ccagaacaaa taaatagatc
     1381 caggaacaga agaaagcaat gaaattcatt tgaatagtta atcgttgtct tacttttaat
     1441 gctttaaatg ctgctttata ttctggtgtt gctccccaga atccaggaaa gccatgttga
     1501 tcataaaaat taaatcggta tttccatttt tcacttaatg aatcatcgtt catatattcc
     1561 ttactcataa atactccata actattgttt tgatgaatca gtaggtgcaa gcattagcat
     1621 actgaaagtg gaaaaataac aaatcaaaaa aatcatcgaa ccattgcctg aacaggcaaa
     1681 atcttcggct atcattgtga tgatagagat gatatatact gctaatgtac caaaaacata
     1741 aatttttata tatatgaaac cactatcacg gagtcgttgg caattcatgt tgatgacgag
     1801 ataatggagt gcgatggtag agactataac aagaaaacct gcttccccat cgttaaaaaa
     1861 gataataaga aaggcaaaaa tgaaatttat taaaataaat aaaaatatat atcggtgtct
     1921 ggaaatctta ccgttagatg ttgggataaa catacgtaac ataaatttta catccttgtg
     1981 tgagtctcct gtcagcatgg caatatgccc actctcatac aagagtcggc atattttttc
     2041 agaatatatt tattttttat ttggacgttc tacgctgcgt ataaatcgcg tccatcacaa
     2101 aaattgccag cgccacccaa ataaaggtga aagtcaccat cttatcggca cccggttttt
     2161 caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac tgaaaaaagc
     2221 ctaacgttga gagacgcagg cgcgtggcag cggcagtaaa acacaacagc ggtacggtag
     2281 tgacaatacc ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc
     2341 tggttgaact gtcggcaata gcaaacaggt aaatcgccgc cacgggcagc agccacatgg
     2401 tttcaattaa catgccggtt tgtgcttcta ccgcaatctt cttgcgcacc aggccgtaga
     2461 aggcaaaact aaatgccagc cccagcgcga taataggtag cgaaccaaaa gtccacagct
     2521 ggaccaacac gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct
     2581 cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc
     2641 tcgcttccag catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa
     2701 tcagtacggc agagactgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta
     2761 aataggacca ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca
     2821 cgcgatgcgt caggatctca tcggcgggca cgtagtaaat cagcttgaag tatgctggcg
     2881 caatacccca aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgttttg
     2941 catccatcgg gaatactcat ttttaattgg taacagcagt ttacctgctt ttatgtcttc
     3001 aacctaccat ataggtggcg gtggcactgg caatataaag ctgttcttca ttgtgtaatt
     3061 caacgcgggc gacggcgact ttattgcctg cacgcaacag gctactggta gcagtaaaac
     3121 gctcgcccct gcctgggcgc agataatcaa cgcgaagatc aatggtcccc atccgcgata
     3181 gccgctggcg tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc
     3241 acaccagacc ggcggcgaca tccagcgccg acgcaatgac tccgccgtgc aaaatgcttt
     3301 gcgcccagtt acccaccatc atcggctgat ttttaaaggc cagctgtgcg aactcttttt
     3361 cgtaacgctc cagttccatc cccaatgcgc ggttaaatgg catgtgataa acaaacatct
     3421 cacccactaa tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacact
     3481 tcattggtta atgaaatgtt gattttatgc ttctttgttg ttggtttcta ctttaggaag
     3541 ggataactaa cggcaatgga gttaagtatg taaaatagcc cgcagaaaaa cattcacctt
     3601 atcaataatt cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgccg
     3661 gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac
     3721 gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc
     3781 acgctctatc cttatgacac caactacctc atctacaccc aaaccagcga tctgaataaa
     3841 gaagcgattg ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag
     3901 ttgagcctgg catttccgct gtggcgtggg attttaggcc caaactcggt gttgggtgcg
     3961 tcttatacgc aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt
     4021 gaaaccaact acgaaccgca attgttcctc ggttttgcca ccgattaccg ttttgcaggt
     4081 tggacgctgc gcgatgtgga gatggggtat aaccacgact ctaacgggcg ttcagacccg
     4141 acctcccgca gctggaaccg cctttatact cgcctgatgg cagaaaacgg taactggctg
     4201 gtagaagtga agccgtggta tgtggtgggt aatactgacg ataacccgga tatcaccaaa
     4261 tatatgggtt actaccagct taaaatcggc tatcacctcg gtgatgcggt gctcagtgcg
     4321 aaaggacagt acaactggaa caccggctac ggcggcgcgg agttaggctt aagttacccg
     4381 atcaccaaac atgtgcgcct ttatactcag gtttacagcg gctatggcga atcgctcatc
     4441 gactataact tcaaccagac ccgtgtcggt gtaggggtta tgctaaacga cttgttttga
     4501 tgaacggttg agtggttggc aaatctggaa tccagcatcc aggattaccc tctcagagac
     4561 taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca
     4621 atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc taaacaggtt
     4681 ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagaaat tatcgacact
     4741 gtgctttccg gtcgcgattg cctggttgtt atgcccactg gtggcggaaa atccctttgc
     4801 tatcaaattc ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg
     4861 ttgatgaaag atcaggtgga tcaactacag gccaacggcg tggcggcggc gtgccttaac
     4921 tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt
     4981 cgcttactgt atatcgcccc ggaacgcctg atgctggata actttcttga gcatctggcg
     5041 cactggaatc cggtgttatt agccgtcgat gaagcgcact gtatctccca atggggtcac
     5101 gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc agcggttccc gacgctgccg
     5161 tttatggcgc tgaccgccac agccgacgac accacgcgcc aggatatcgt gcgcctgctg
     5221 gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat tcgctacatg
     5281 ctgatggaga aattcaaacc gctcgatcag ttgatgcgct acgtgcagga acagcgcggt
     5341 aagtctggca ttatctactg caacagccgg gcgaaagtag aagacaccgc tgcgcgcctg
     5401 caaagcaggg gtattagcgc ggcggcctat catgccgggc tggaaaataa tgtccgcgcc
     5461 gacgtgcagg agaaattcca gcgcgatgat ctgcaaattg tggtggcgac ggtggcgttc
     5521 ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat
     5581 atcgaatcct attatcagga aaccggtcgc gccgggcgtg atggcctgcc tgcggaagcg
     5641 atgctgtttt acgatccggc tgatatggcg tggctgcgcc gttgtctgga agagaagccg
     5701 caggggcagt tgcaggatat cgagcgccac aaactcaatg cgatgggcgc gtttgccgaa
     5761 gcgcaaactt gccgtcgtct ggtattgctg aactattttg gcgaagggcg tcaggagccg
     5821 tgcgggaact gcgatatctg cctcgatccg ccgaaacagt acgacggttc aaccgatgct
     5881 cagattgccc tttccaccat tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg
     5941 gaagtgattc gtggtgctaa taaccagcgt atccgcgact atggtcatga caaactgaaa
     6001 gtctatggta tgggccgtga taaaagccat gaacattggg tgagcgtgat ccgccagctg
     6061 attcatctcg gcctggtgac gcaaaatatt gcccagcatt ctgccctaca actgacagag
     6121 gccgcgcgcc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg
     6181 gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg caaactgttc
     6241 gccaaattac gcaaactgcg taaatcgatt gccgatgaaa gtaatgtccc gccgtacgtg
     6301 gtgtttaacg acgcaacctt gattgagatg gctgaacaga tgccgatcac cgccagcgaa
     6361 atgctcagcg ttaacggcgt tgggatgcgc aagctggaac gctttggtaa accgtttatg
     6421 gcgctgattc gtgcgcatgt cgatggcgac gacgaagagt agtcagcagc ataaaaaagt
     6481 gccagtatga agactccgta aacgtttccc ccgcgagtca aatgtatgtt gatgttattt
     6541 ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc ccggtcccga tttctttttt
     6601 gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt
     6661 acctgcggcg taatggtttg ggctgggatt gcgttgcttg gcctgcattt gattatcgaa
     6721 aaaatggcct ggctgcatac gctgattatg gtaggcggtg gcctgtatct ctgctggatg
     6781 ggttaccaga tgctacgcgg tgcactgaaa aaagaggctg tatctgcgcc tgcaccacag
     6841 gttgagctgg cgaaaagcgg gcgcagtttc ctgaaaggct tactgaccaa tctcgccaat
     6901 ccgaaagcga ttatctattt tggttcggta ttctcattgt ttgtcggcga taacgttggc
     6961 actaccgagc gctggggcat tttcgcgttg attattgtcg aaacactggc gtggtttacc
     7021 gtcgttgcca gtctgtttgc cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag
     7081 tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt gattatttcg
     7141 cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc gcatccgaca
     7201 ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata aacaacgagc
     7261 cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga gcaatccttt
     7321 gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg gtgacgccga
     7381 gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga aatagcgccg
     7441 ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc tggaacaaat
     7501 gtcgacgcga ttgagtagag gccagcgatt taagatcaat tgcgccagcg gcgcgccact
     7561 gctggattcc cagccaaatc aagtaagccg cgcctgccca ctttaacact tcaaacgcaa
     7621 tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata tgaatcgcca
     7681 gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg
     7741 tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc gatgtcagca
     7801 ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca
     7861 caatacgcta ctgtcgcagc gttgtgtcag gcacgcttaa aaaaacgatt ttacgtggtt
     7921 taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag agaaaacgcg
     7981 tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga tgaagcagag
     8041 tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac
     8101 cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc ggaactggcc
     8161 tacgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt
     8221 tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa tgattatgtt
     8281 gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg taaacgctat
     8341 atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg ccatccaggt
     8401 gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg tatgccgtca
     8461 tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg tgatggctat
     8521 gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc
     8581 agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat tcgcgtcggt
     8641 gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt gttagccggt
     8701 gcgggtgatg acgccacgca aacgcttctc ttacaagctg aagaggaacg cgtggtggat
     8761 aaccgcatgc acgaccgttt ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga
     8821 ggacggccgt tggtaataaa aggtgcttac catgagatcc tttttgaaaa ggacgcaatg
     8881 cgctcagtcg cgctccacgc catcgttgat tttttcaaca ggcataactc acccagcgga
     8941 aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg atttagatgg
     9001 cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaactc tgaagctgct
     9061 caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg atgtggggca
     9121 aattcgcgat aatctggaga ttaagtctta catgattacc tccaatggtg cgcgcgttca
     9181 cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg ccagcgatct
     9241 gtttggcgta gtcaacgaca atccggacat cattactaac gtttatcgcg acgacgaatg
     9301 gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg tgttcaaata
     9361 tgcgctgtat gagccgggat tactggagcc ggaaggcatc agcaaagtgt ttttcacctg
     9421 cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgct ggggcgatcg
     9481 cgtcaacgtc agtttctcta ccttaacctg tctggaagtg atggcgggcg gcgtgtcgaa
     9541 aggccatgcg ctggaagcag tggcgaagaa actgggctac agcctgaagg attgtattgc
     9601 gtttggtgac gggatgaacg acgccgaaat gctgtcgatg gcggggaaag gctgcattat
     9661 gggcagtgcg caccagcgtc tgaaagatct gcatccggag ctggaagtta ttggtactaa
     9721 tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat cgttctttat
     9781 ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca aagagttttc
     9841 cattgtggcg ttacttatca tcaccacgat tctgtgggcc ttctccttta gcttttatgg
     9901 cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg ttggcttggc
     9961 ggcactcgtt tttctgccgt ttctgcgtac ccgtagcaat agcctgaaaa cggtcggcct
    10021 gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt tccgcgctta
    10081 tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc tctacatcac
    10141 gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt ttagcgcctt
    10201 gctggcggtg attggtgccg ggattattcg ctatgatcag gtcaccgacc atttctggac
    10261 tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg tgggttacaa
    10321 acgcctgatg gaaactcgcc cgatgccgca gtataacgcc tttgcgtggt tctatcttgg
    10381 cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga aaatgccgca
    10441 aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg ggattggcta
    10501 ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca ttatgaataa
    10561 tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac agccgcactg
    10621 gccaacgttt attacaggcg cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg
    10681 ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga
    10741 ataaacgctt ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg
    10801 ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc aaaactctct
    10861 actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatttg aatcaacaat
    10921 aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc
    10981 cagacatcca gtcgactacg ctgcaccgga taaattaata gcgtctcgct ggcgagatct
    11041 tccggtgtaa tacgcgtttt tgccgccagt ggatggtcag gtgctaacac caggcgtact
    11101 tcataatcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc ggaggtcatc
    11161 accagatcca gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac gcccgattta
    11221 aaatccatct ctacctgcgg ccagttctta tggaaatttc ctaaagcggg tgtcagccac
    11281 tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag
    11341 gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa caggatttct
    11401 ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa gccaaggcgt
    11461 tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa cgttgccgca
    11521 gcggctgcga gcgagccgca gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg
    11581 atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat acagtaaccg
    11641 ccaattatgg atgtgtaaac atctggacgg ctaaaatcat tcgtctttta aatttatggt
    11701 gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg cgatgaataa
    11761 acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag aggaagaaaa
    11821 atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa
    11881 aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct gacagtaggg
    11941 cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga cctgctgccg
    12001 gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct gggtaatgtt
    12061 ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt ccgtattggt
    12121 cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac
    12181 accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa actgacttgg
    12241 acgcagctgc tggaggaagt ggacgaggcg ctggcgctga gccacaacgt taagcctgtg
    12301 ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc
    12361 cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga actggcgaaa
    12421 cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact accacaggcg
    12481 tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa actgctgctg
    12541 accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct gcctgttcag
    12601 gggctgcatg tggaccttgt acatggtaaa gataacgttg ttgaactgca caagcgcctg
    12661 ccttctgact ggctgctgtc tgcgggtctg atcaatggtc gtaacgtctg gcgcgccgat
    12721 cttactgaga aatatgcgca aattaaggac attgtcggca aacgtgattt gtgggtggca
    12781 tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg tcttgatgca
    12841 gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactggc actgctgcgc
    12901 gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc aattcaggcg
    12961 cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc ggcgatcacc
    13021 gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt
    13081 tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac cacggaaatt
    13141 cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaacaacta ccgcacgggc
    13201 attgcggaac atatcaagca ggccattgtt gagcaggaac gtttgggact ggatgtgctg
    13261 gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca cctcgacgga
    13321 tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt gaagccaccg
    13381 attgtcattg gtgacattag ccgcccggca ccgattaccg tggagtgggc gaagtatgcg
    13441 caatcgctga ccgacaaacc ggtgaaaggg atgctgacgg ggccggtgac catactctgc
    13501 tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat tgcgctggcg
    13561 ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca gattgacgaa
    13621 ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta tctccagtgg
    13681 ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca aatccacact
    13741 cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcgct ggacgcagac
    13801 gtcatcacca tcgaaacctc gcgttccgac atggagttgc tggagtcgtt tgaagagttt
    13861 gattatccaa atgaaatcgg tcctggcgtc tatgacattc actcgccaaa cgtaccgagc
    13921 gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc agagcgcctg
    13981 tgggtcaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcggcactg
    14041 gcgaacatgg tgcaggcggc gcagaatttg cgtcgggggt aaatccaaac cgggtggtaa
    14101 taccacccgg tcttttctca ttacagcgac ttcttcccac catactgctt aaaccattcc
    14161 agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcagcg
    14221 ttgaatgcat gcccggcatc cgggtacacg ataatctctg ctttcgcatt agcagcccgc
    14281 agcgcctggc gcatcgtttc aacgctctcc tgcggaatgc tgttatcctg accaccatat
    14341 aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc
    14401 agcgacttgt cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta
    14461 tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga
    14521 tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc
    14581 tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca
    14641 ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc ctccagcgcc
    14701 agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg
    14761 accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc
    14821 ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt ctgaacgatg
    14881 gtcgaagcga gaggcgatgc agcaggtgca aatccagatt gttgtgttgt tgccatggta
    14941 ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc
    15001 acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg
    15061 atatgaatca cttttggtgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat
    15121 cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat
    15181 tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg
    15241 atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg
    15301 ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcact acctggcgtg
    15361 cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct
    15421 ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa
    15481 cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc
    15541 gtctggatgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg
    15601 aatgtacgac tgcgctggtt gaagctgcga aatctattgg cgcgacaact cacgttggcg
    15661 tgacagtttc ttctgatacc ttctacccag gtcaggaacg ttacgatact tactctggtc
    15721 gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact
    15781 atgaaatgga atctgcaact ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta
    15841 tggtagcggg tgttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga
    15901 aacaaaccga aagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat
    15961 tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg
    16021 caggaaatgc ctttccaact ggacgtttgt acagcacaat tctattttgt gcgggtaagt
    16081 tgttgcgtca ggaggcgttg tggatttctc aatcatggtt tacgcagtta ttgcgttggt
    16141 gggtgtggca attggctggc tgtttgccag ttatcaacat gcgcagcaaa aagccgagca
    16201 attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca
    16261 aagcgagcac tggcgtgcag agtgtgaatt gctcaataac gaagtgcgca gcctgcaaag
    16321 tattaacacc tctctggagg ccgatctgcg tgaagtaacc acgcggatgg aagccgcaca
    16381 gcaacatgct gacgataaaa ttcgccagat gattaacagc gagcagcgcc tcagtgagca
    16441 gtttgaaaac ctcgccaacc gtatttttga gcacagcaat cgccgggttg atgagcaaaa
    16501 ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg gtttccgccg
    16561 tcaggttcag gacagcttcg gtaaagaagc acaagaacgc cataccctga cccacgaaat
    16621 tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc tgacgcgcgc
    16681 gctgaaaggc gacaataaaa cccagggcaa ctggggcgag gtagtattga cgcgggtgct
    16741 ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc caggtcagca tcgaaaatga
    16801 cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg ccgcagggaa aagatgtggt
    16861 gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat tttaacgccg aagacgacta
    16921 cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg cgtaaccata tccgtttgct
    16981 gggacgcaaa gattatcaac agctgccggg gctgcgaact ctggattacg tgctgatgtt
    17041 tattcccgtt gaacccgctt ttttactggc gcttgaccgc cagccggagc tgatcaccga
    17101 agcgttgaaa aacaatatca tgctggttag cccgactacg ctgctggtgg cgttgcgtac
    17161 tattgccaac ctgtggcgtt atgagcatca aagccgtaac gcccagcaaa tcgccgatcg
    17221 tgccagcaag ctgtacgaca agatgcgttt gttcatcgat gacatgtccg cgattggtca
    17281 aagtctcgac aaagcgcagg ataactaccg ccaggcaatg aaaaaactct cttcaggtcg
    17341 cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa ttaaacgcga
    17401 gattaatccg gatttggctg aacaggcggt gagccaggat gaagagtatc gacttcggtc
    17461 ggttccggag cagccgaatg atgaagctta tcaacgcgat gatgaatata atcagcagtc
    17521 gcgctagccc attaggggta gttaagccgg gtagaaatct agggcatcga cgcccaatct
    17581 gttacacttc tggaacaatt ttttgatgag caggcattga gatggtggat aagtcacaag
    17641 aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc
    17701 acgttttcca ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg tcatttggta
    17761 ttcatcgttt gtggaagcga ttcacgattg attgcagcgg cgtacgccgt gggcagaccg
    17821 tgctggatct ggctggtggc accggcgacc tgacagcgaa attctcccgc ctggtcggag
    17881 aaactggcaa agtggtcctt gctgatatca atgaatccat gctcaaaatg ggacgcgaga
    17941 agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc
    18001 tgccgttccc ggataacacc tttgattgca tcaccatttc gtttggtctg cgtaacgtca
    18061 ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc
    18121 tggtgcttga gttctcgaag ccaattatcg agccgctgag caaagcctat gatgcatact
    18181 ccttccatgt gctgccgcgt attggctcac tggtcgcgaa cgacgccgac agctaccgtt
    18241 atctggcaga atccatccgt atgcatcccg atcaggatac cctgaaagcc atgatgcagg
    18301 atgccggatt cgaaagtgtc gactactaca atctgacggc aggggttgtg gcgctgcatc
    18361 gtggttataa gttctgacag gagaccggaa atgcctttta aacctttagt gacggcagga
    18421 attgaaagtc tgctcaacac cttcctgtat cgctcacccg cgctgaaaac agcccgctcg
    18481 cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc attgattctg
    18541 gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg caggcgatgc tgactgcacc
    18601 gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc gccagcagct taccgcactg
    18661 attcgcagtg gtgagctgga agtgcagggc gatattcagg tggtgcaaaa cttcgttgcg
    18721 ctggcagatc tggcagagtt cgaccctgcg gaactgctgg ccccttatac cggtgatatc
    18781 gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca tcacggcatt
    18841 aagcgccagc aacgttatgt ggcggaagcc attactgaag agtggcgtat ggcacccggt
    18901 ccgcttgaag tggcctggtt tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc
    18961 ctgaccaaac ggctggaaaa actggaggct aaatgacgcc aggtgaagta cggcgcctat
    19021 atttcatcat tcgcactttt ttaagctacg gacttgatga actgatcccc aaaatgcgta
    19081 tcaccctgcc gctacggcta tggcgatact cattattctg gatgccaaat cggcataaag
    19141 acaaactttt aggtgagcga ctacgactgg ccctgcaaga actggggccg gtttggatca
    19201 agttcgggca aatgttatca acccgccgcg atctttttcc accgcatatt gccgatcagc
    19261 tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag cagcagattg
    19321 aagctgcaat gggcggcttg ccggtagaag cgtggtttga cgattttgaa atcaagccgc
    19381 tggcttctgc ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg
    19441 tggtgattaa agtcatccgc ccggatattt tgccggtcat taaagcggat ctgaaactta
    19501 tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa
    19561 ccgaagtggt gcgcgagtac gaaaagacct tgattgatga actgaatttg ctgcgggaat
    19621 ctgccaacgc cattcagctt cggcgcaatt ttgaagacag cccgatgctc tacatcccgg
    19681 aagtttaccc tgactattgt agtgaaggga tgatggtgat ggagcgcatt tacggcattc
    19741 cggtgtctga tgttgcggcg ctggagaaaa acggcactaa catgaaattg ctggcggaac
    19801 gcggcgtgca ggtgttcttc actcaggtct ttcgcgacag ctttttccat gccgatatgc
    19861 accctggcaa catcttcgta agctatgaac acccggaaaa cccgaaatat atcggcattg
    19921 attgcgggat tgttggctcg ctaaacaaag aagataaacg ctatctggca gaaaacttta
    19981 tcgccttctt taatcgcgac tatcgcaaag tggcagagct acacgtcgat tctggctggg
    20041 tgccaccaga taccaacgtt gaagagttcg aatttgccat tcgtacggtc tgtgaaccta
    20101 tctttgagaa accgctggcc gaaatttcgt ttggacatgt actgttaaat ctgtttaata
    20161 cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt gttactccag aaaaccctgc
    20221 tctatgtcga aggggtagga cgccagcttt atccgcagct cgatttatgg aaaacagcga
    20281 agcctttcct ggagtcgtgg attaaagatc aggtcggtat tcctgctctg gtgagagcat
    20341 ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa ctggtttacg
    20401 acagtttgcg ccagggcaag tatttacagc acagtgttga taagattgcc cgcgagcttc
    20461 agtcaaatca tgtacgtcag ggacaatcgc gttattttct cggaattggc gctacgttag
    20521 tattaagtgg cacattcttg ttggtcagcc gacctgagtg ggggctgatg cccggctggt
    20581 taatggcagg tggtctgatc gcctggtttg tcggttggcg caaaacacgc tgattttttc
    20641 atcgctcaag gcgggccgtg taacgtataa tgcggctttg tttaatcatc atctaccaca
    20701 gaggaacatg tatgggtggt atcagtattt ggcagttatt gattattgcc gtcatcgttg
    20761 tactgctttt tggcaccaaa aagctcggct ccatcggttc cgatcttggt gcgtcgatca
    20821 aaggctttaa aaaagcaatg agcgatgatg aaccaaagca ggataaaacc agtcaggatg
    20881 ctgattttac tgcgaaaact atcgccgata agcaggcgga tacgaatcag gaacaggcta
    20941 aaacagaaga cgcgaagcgc cacgataaag agcaggtgta atccgtgttt gatatcggtt
    21001 ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt tctggggccg caacgactgc
    21061 ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg
    21121 tgcagaacga actgacccag gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg
    21181 ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa agcgtcgatg gatgaactac
    21241 gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg
    21301 atgaagcgca caccatccat aacccggtgg tgaaagataa tgaagctgcg catgagggcg
    21361 taacgcctgc cgctgcacaa acgcaggcca gttcgccgga acagaagcca gaaaccacgc
    21421 cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt
    21481 cgtcgagtga taaaccgtaa acatgtctgt agaagatact caaccgctta tcacgcatct
    21541 gattgagctg cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga tattcctgtg
    21601 tctggtctat ttcgccaatg acatctatca cttggtatcc gcgccattga tcaagcagtt
    21661 gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa
    21721 gctgaccttt atggtgtcgc tgattctgtc agcgccggtg attctctatc aggtgtgggc
    21781 atttatcgcc ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc tgctggtttc
    21841 cagctctctg ctgttttata tcggcatggc attcgcctac tttgtggtct ttccgctggc
    21901 atttggcttc cttgccaata ccgcgccgga aggggtgcag gtatccaccg acatcgccag
    21961 ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg aagtgccggt
    22021 ggcaattgtg ctgctgtgct ggatggggat tacctcgtca gaagacttac gcaaaaaacg
    22081 cccgtatgtg ctggttggtg cattcgttgt cgggatgttg ctgacgccgc cggatgtctt
    22141 ctcgcaaacg ctattggcga tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc
    22201 acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg aagcagaaag
    22261 cgaaaaagct gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc
    22321 ggttgtcata tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt
    22381 gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc
    22441 atcaccggca ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg
    22501 tcctgttggt caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact
    22561 gaagaagcga ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt
    22621 ctcgacttta accgtaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag
    22681 ctacgcattg ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag
    22741 cggtttatga cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc
    22801 tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt
    22861 accggttggg tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt
    22921 ccggcggaga aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg
    22981 ccaaagccat catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt
    23041 gcgcactggc gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa
    23101 acactgtttg ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta
    23161 atctctttat taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg
    23221 aaaatggcct gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa
    23281 gggggggccg gatcgacaat gtctttcggt ttatataccg atagctgatg aattaccgcc
    23341 gatgggacta tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg
    23401 ctgatggtcg tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat
    23461 aacggttcac tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga
    23521 gcgaggcaat taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc
    23581 ttgcagtaca gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa
    23641 ggatagcaaa agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga
    23701 gttcacgcta atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca
    23761 gattaagagg ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg
    23821 cttgaacagc agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc
    23881 actgaaattg ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct
    23941 aaaggctact caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg
    24001 ggcatggggc aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg
    24061 aaagagccgg agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag
    24121 caggtgttaa acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc
    24181 tctggcgatg acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc
    24241 gcgccgctga ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat
    24301 ctgggcattt atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg
    24361 catcgcggcg gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc
    24421 ccggtttctg tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt
    24481 ccggatacgc tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta
    24541 gtaaagtgta tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg
    24601 tatatcgatc ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat
    24661 aatgaagtcg ataatttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg
    24721 atttaccatt ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg
    24781 ttgaacgaag tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac
    24841 ctgccgccgg aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc
    24901 ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact
    24961 aaatttgtga tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg
    25021 gcgattacca cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt
    25081 gattatctgg attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc
    25141 acgaataaat ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca
    25201 gatgttgtcg cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa
    25261 agcgcctgat gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca
    25321 accttaagct gtaaagtgac ctcggtagca gctatcacgg ataccgtata tcgtgtccgc
    25381 atcgtgccag acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat
    25441 gagcgcgaca aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag
    25501 ctgcatattg gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc
    25561 aaagatcatc aaatcgtggt cgaccttccc cacggagaag cgtggctgcg cgatgatgaa
    25621 gagcgtccga tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg
    25681 ctgacagcgt tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa
    25741 gagcagcatc tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccttggtctg
    25801 caagtggtgc cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg
    25861 ttaacggcgg tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga
    25921 cgttttgaga tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa
    25981 gatcgcctgt ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac
    26041 aggcgggaag aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg
    26101 gcaattggtg cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc
    26161 cggatcggta gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca
    26221 aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg
    26281 tctttgcgtt ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acaacccagc
    26341 ggatgaccca gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt
    26401 agcccgagat ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac
    26461 acgccgatat cgctggcaga aagccccgct tttttcagcg ccagcttcga ggccggaacc
    26521 gggccgtaac ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg
    26581 cgcggcttaa gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg
    26641 ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc
    26701 ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta
    26761 aactgcttca ggacgccgtc ggcatcgtga ccaccagttg ggatgatttc atttttaaat
    26821 gcggccgact gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc
    26881 atttcacggc tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc
    26941 gccgctttgg cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc
    27001 acatggccta tatgctccac gccgccaacc agacatgcct gcgtatcgcc agtcatgatc
    27061 attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc
    27121 gccgggacag agtgcggcac ttctgccagc agcgccgcgt tacgggcgat gttaaaaccc
    27181 tgctccagcg tctgttgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc
    27241 gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc cgcacgcacg
    27301 ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca
    27361 acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca
    27421 accggaggat agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca
    27481 tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg
    27541 gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc
    27601 gccatatccg cttccgccgg agtggcgata atgccctctt ccagacagcg caccacttcg
    27661 ttgaccatcg ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggt
    27721 tggctcactt ctgccagcag gtcttcaacg gcggcgtctt cttctttctt cggcttacct
    27781 ttgctgtctt ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca
    27841 tcaaacagcg cgtcgatggc atcgcggtaa tctttctgca tccgctgcgg gaagcctgct
    27901 gccatgacag cctgagcgtg atgcgcggta tcaatgccca caacgtccag cagatatgcc
    27961 gggcccatcg gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg
    28021 ccgtcgcgca gcagctggct gaaaccggcg aaatacggga acagcacgcg gttaacaaag
    28081 aagccggggc agtcgttaac cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca
    28141 actttcgcga tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatt
    28201 cggtggaccg ggttaaagaa gtgcatcccg cagaagtttt ccgggcgttc cagcgcgttg
    28261 gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc ctggcgtact
    28321 ttttgttcgg tttctgccag tacggctttt ttcactttcg ggttttcaac aaccgcttct
    28381 accacaatat ccacgcggtc aaatccggcg tagtcgagcg ttgggtggat cgtggagatc
    28441 acgccagcca gtttcagacc atcgattttg ccgcgctcaa gctgcttgtt cagcagtttc
    28501 gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc
    28561 acgcctttcc acgcggactg gtaagcgata ccgccaccca tgatgcctgc gcccagcaca
    28621 gcagcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat
    28681 tgatcgttaa ggaaaatgcc gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca
    28741 aaacttttgt tttccagatt taaggcttct tcacgaccaa aacgggccgc agcttcaatg
    28801 gtttttactg cggtgatggg ggccggataa tgtttccccg ccgtttgcgc gaccattcct
    28861 ttagcgatgg tgaagctcat ggtggcttca atcttgctca gttttagcgg ttcctgcttc
    28921 ggctgacgtt tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aatcgccatc
    28981 gccccttcaa tcagtttttc tgctttgact acgccatcca ccagaccgat tttcagcgcc
    29041 tgatccgcgc cgacatcttt accggcggca atgatttcca gtgcactgtc agcgcccagc
    29101 atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc
    29161 agaccgatgc gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag
    29221 ccaccaccca gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg
    29281 cgattaaaca cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg
    29341 aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc
    29401 agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg
    29461 gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca
    29521 atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc
    29581 tgaatccgca aggtaatctg gtcgtaccag atgcatcgga gtgtgtgttt tgtgttaaaa
    29641 atatgcaaat aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc
    29701 tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa
    29761 tggaatcact ggcctcgctc tataaaaatc atatagctac cttacaagaa cggactcgcg
    29821 atgcgctggc gcgcttcaag ctggatgcat tacttattca ctccggcgag ctgttcaacg
    29881 tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc
    29941 cggtaactca ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt
    30001 ggttctatct gccggttgat tactggcaca acgtcgaacc gctgccgacc tccttctgga
    30061 ctgaagatgt ggaagtgatc gcgctgccga aagccgatgg cattggtagc ctgctgcctg
    30121 ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga acgtgcgctg caactgggta
    30181 ttgaggccag caacatcaac ccgaaagggg tgatcgacta cctgcattat taccgctcct
    30241 tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaatggtc
    30301 atcgtgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc aatattgcct
    30361 atctgaccgc gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta
    30421 acgaacacgc tgcggtgttg cattacacca aactggacca ccaggcaccg gaagagatgc
    30481 gcagcttcct gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgacccgta
    30541 cctggtcggc aaaaagcgac aacgactacg cacagctggt gaaagacgta aatgatgaac
    30601 aactggcgct gatcgccacc atgaaagcag gcgtcagcta tgtggattac cacatccagt
    30661 tccatcagcg catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag
    30721 aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc gcacggtatt ggccatccgc
    30781 tgggcctgca ggtgcatgac gtcgccggtt ttatgcagga tgatagcggt acacacctcg
    30841 cggcaccggc aaaatatccg tacctgcgct acacccgtat tctccagccg ggcatggtgt
    30901 taaccatcga accgggtatc tacttcattg aatcgcttct ggcaccgtgg cgtgaagggc
    30961 agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc
    31021 gtatcgaaga caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga
    31081 aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat
    31141 caaaaagagc cgcttcataa cgatgttggc gcataccgat ggcgttgagg cggcgaaagc
    31201 gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt
    31261 ggcgggtgcg ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcggg
    31321 aacggcaggt aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc
    31381 tgtggtagtg cgctactacg gcggcatatt gcttggcacc ggtgggttag tgaaagcgta
    31441 tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac
    31501 cgaatatact ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca
    31561 gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct
    31621 tccggcggcg aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt
    31681 gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca
    31741 gagatgcatt ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg
    31801 accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt
    31861 acccagacct tttttgtcgc cctcgccatt ggctctatgc tgtggtggcc gaaccgcaaa
    31921 gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ttggaccgtg
    31981 ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc
    32041 gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg
    32101 ctggattcgc tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg
    32161 atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag
    32221 ctctatcgcg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg
    32281 gaaacggcga aaactctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg
    32341 ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct
    32401 attggcggct tctcgacaca tgatgccagt atcggttatt tcgacagtcc gactattaac
    32461 actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg
    32521 ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc
    32581 gtacagttta cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt
    32641 tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc
    32701 gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt
    32761 tcagctttta tcggcggttg cgccgggtca acgggcggtg gcctgaaagt gatccgcatc
    32821 ctgctgctgt ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc
    32881 tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga
    32941 tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc
    33001 gtggatgatt tttctgcctt tgcctcagtt gttgcgacat tgaataacct ggggccaggg
    33061 cttggcgtgg ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt
    33121 gccaacatgc tgtttggtcg tctcgaggtc tttacattac tggtgctctt taccccgact
    33181 ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg
    33241 gacaaacgcg cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccaga
    33301 cggatgtcgc caatgtgcac cgcattgaag aaccacaatg ggaaaactat gaccgtgtgg
    33361 tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa
    33421 aacatgcgac gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc
    33481 gcaaaccgga gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact
    33541 cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc
    33601 gctggtacga ccgttttatg ctcaagctga ttatgaagat gtcaggcggt gaaacggata
    33661 cgcgcaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg
    33721 cccatttaac cgacaaaccg acgctgaaat aagcataaag aataaaaaat gcgcggtcag
    33781 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
    33841 gacacggaac aacggcaaac acgcagccgg gtcagcgggg ttctcctgag aattccgaca
    33901 gagaaagcaa aaataaatgc ttgactttgt agcgggaaag cgtattatga acaccccgcg
    33961 c
//
LOCUS       DAJTYL010000034        33836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYL010000034 DAJTYL010000000
VERSION     DAJTYL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.034"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..539)
                     /locus_tag="OO572_001566"
     CDS             complement(<1..539)
                     /locus_tag="OO572_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6660611.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALP"
     gene            631..717
                     /locus_tag="OO572_001567"
                     /pseudo
     CDS             631..717
                     /locus_tag="OO572_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1122..1598)
                     /locus_tag="OO572_001568"
     CDS             complement(1122..1598)
                     /locus_tag="OO572_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6660612.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1657..2946)
                     /gene="bioA"
                     /locus_tag="OO572_001569"
     CDS             complement(1657..2946)
                     /gene="bioA"
                     /locus_tag="OO572_001569"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6660613.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3033..4073
                     /gene="bioB"
                     /locus_tag="OO572_001570"
     CDS             3033..4073
                     /gene="bioB"
                     /locus_tag="OO572_001570"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6660614.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4070..5224
                     /gene="bioF"
                     /locus_tag="OO572_001571"
     CDS             4070..5224
                     /gene="bioF"
                     /locus_tag="OO572_001571"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6660615.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5211..5966
                     /gene="bioC"
                     /locus_tag="OO572_001572"
     CDS             5211..5966
                     /gene="bioC"
                     /locus_tag="OO572_001572"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6660616.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5959..6636
                     /gene="bioD"
                     /locus_tag="OO572_001573"
     CDS             5959..6636
                     /gene="bioD"
                     /locus_tag="OO572_001573"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6660617.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7215..9236
                     /gene="uvrB"
                     /locus_tag="OO572_001574"
     CDS             7215..9236
                     /gene="uvrB"
                     /locus_tag="OO572_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6660618.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9428..10336)
                     /gene="yvcK"
                     /locus_tag="OO572_001575"
     CDS             complement(9428..10336)
                     /gene="yvcK"
                     /locus_tag="OO572_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6660619.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10601..10745
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10733..11722
                     /gene="moaA"
                     /locus_tag="OO572_001576"
     CDS             10733..11722
                     /gene="moaA"
                     /locus_tag="OO572_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6660620.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11744..12256
                     /gene="moaB"
                     /locus_tag="OO572_001577"
     CDS             11744..12256
                     /gene="moaB"
                     /locus_tag="OO572_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6660621.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12259..12744
                     /gene="moaC"
                     /locus_tag="OO572_001578"
     CDS             12259..12744
                     /gene="moaC"
                     /locus_tag="OO572_001578"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6660622.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12737..12982
                     /gene="moaD"
                     /locus_tag="OO572_001579"
     CDS             12737..12982
                     /gene="moaD"
                     /locus_tag="OO572_001579"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6660623.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12984..13436
                     /gene="moaE"
                     /locus_tag="OO572_001580"
     CDS             12984..13436
                     /gene="moaE"
                     /locus_tag="OO572_001580"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6660624.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13573..14277
                     /locus_tag="OO572_001581"
     CDS             13573..14277
                     /locus_tag="OO572_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6660625.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14482..15195
                     /locus_tag="OO572_001582"
     CDS             14482..15195
                     /locus_tag="OO572_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6660626.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15231..16187)
                     /locus_tag="OO572_001583"
     CDS             complement(15231..16187)
                     /locus_tag="OO572_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6660627.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16187..17428)
                     /gene="clsB"
                     /locus_tag="OO572_001584"
     CDS             complement(16187..17428)
                     /gene="clsB"
                     /locus_tag="OO572_001584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6660628.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17425..18186)
                     /locus_tag="OO572_001585"
     CDS             complement(17425..18186)
                     /locus_tag="OO572_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6660629.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18319..18729
                     /locus_tag="OO572_001586"
     CDS             18319..18729
                     /locus_tag="OO572_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6660630.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18691..19797)
                     /locus_tag="OO572_001587"
     CDS             complement(18691..19797)
                     /locus_tag="OO572_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6660631.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19808..20941)
                     /locus_tag="OO572_001588"
     CDS             complement(19808..20941)
                     /locus_tag="OO572_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6660632.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20934..22670)
                     /locus_tag="OO572_001589"
     CDS             complement(20934..22670)
                     /locus_tag="OO572_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6660633.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22663..23658)
                     /gene="hlyD"
                     /locus_tag="OO572_001590"
     CDS             complement(22663..23658)
                     /gene="hlyD"
                     /locus_tag="OO572_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6660634.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23661..24332)
                     /gene="cecR"
                     /locus_tag="OO572_001591"
     CDS             complement(23661..24332)
                     /gene="cecR"
                     /locus_tag="OO572_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6660635.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24561..25748
                     /gene="rhlE"
                     /locus_tag="OO572_001592"
                     /pseudo
     CDS             24561..25748
                     /gene="rhlE"
                     /locus_tag="OO572_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(25955..26437)
                     /gene="ybiA"
                     /locus_tag="OO572_001593"
     CDS             complement(25955..26437)
                     /gene="ybiA"
                     /locus_tag="OO572_001593"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6660636.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26557..28707
                     /gene="dinG"
                     /locus_tag="OO572_001594"
     CDS             26557..28707
                     /gene="dinG"
                     /locus_tag="OO572_001594"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6660637.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28735..29697
                     /gene="ybiB"
                     /locus_tag="OO572_001595"
     CDS             28735..29697
                     /gene="ybiB"
                     /locus_tag="OO572_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6660638.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29838..30923
                     /gene="hcxB"
                     /locus_tag="OO572_001596"
     CDS             29838..30923
                     /gene="hcxB"
                     /locus_tag="OO572_001596"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6660639.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31152..31412)
                     /locus_tag="OO572_001597"
     CDS             complement(31152..31412)
                     /locus_tag="OO572_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6660640.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31677..31943)
                     /gene="ybiI"
                     /locus_tag="OO572_001598"
     CDS             complement(31677..31943)
                     /gene="ybiI"
                     /locus_tag="OO572_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6660641.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32017..32694)
                     /gene="ybiX"
                     /locus_tag="OO572_001599"
     CDS             complement(32017..32694)
                     /gene="ybiX"
                     /locus_tag="OO572_001599"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6660642.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32736..>33836)
                     /locus_tag="OO572_001600"
     CDS             complement(32736..>33836)
                     /locus_tag="OO572_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR6660643.1"
                     /translation="STGVEFTRETQTNYGVNPVTLPAVNIYHPDSRIHPGGLTRNGAN
                     ANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVA
                     KGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGN
                     SANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKR
                     VEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQ
                     ATDDISVGAGARYIGSIHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNL
                     FDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
BASE COUNT         8027 a         8702 c         9131 g         7976 t
ORIGIN      
        1 tctggtaagg cagtaagctg gttgttgcca acagaaagtt tttgtagagc cgggggaaga
       61 tcaggtagcg tctccaggct attgagaagg gctgagagtg actgcaagga agatggaaga
      121 gaaggcaaac atgttaaaac tctgttaagt gaaacatcaa gagcgaccag gtgaggcgga
      181 agagccggga gcctgctcag tctgttatcg ctggctttaa gtacagttaa ggacggaggg
      241 agttctggca gagagcgtag ctcattgcta gagatgttaa gttcttgtat gtgcgggggc
      301 aggtatggga gagagcgtaa tctgagtaaa cttaaattga gggctggctc ttgaaaagcc
      361 agacatattt tcagtaatcg aactgcctgt gttcggtctt ctgttgcagc accttccttg
      421 gcccagttat cccagatgcg gtgataatcc gtgatatttt gctcttctac ggataaacgg
      481 gatatacatc ctaaaccaca aaaggagcgg aaaaaagaag gattgtgggc aggtttcatt
      541 tattattgat cctcattgtt tatatattaa tatattcgtg caaatggagt gaggattctt
      601 taaaaaaacg cgcctgtaaa tagttaagaa caggaagaac tgttggggat gtggctggcc
      661 gaaaatgaag gggtgaagtt ctggctcaat gtgctgactg aactgaaaaa acgcggttgg
      721 tcgccctacc ttttcatcaa gccagtagtc tgcccaccac agcatcattt ctctgcgctt
      781 atcgagatac tgagcatggt tgtaaattca gctaacccag tgcgttgaca aaaaattagc
      841 gcaagaggac aaaaaatcac cttgcgctaa tgctctgtat caggtcacta ataccatcta
      901 agtggttgat tcatagtgac tggatatgtt gtgttttgca gtattatgca gtctattttt
      961 taagctaaat gtattataat gcattgatat taatgatttt tactgtttct cggtcagctt
     1021 ttttatacta acttgagcga aacgggaagg taaaaagaca aaaagttgtt tttaatacct
     1081 ttaagtgata ccagatggca ttgcgccatc tggcagagtg attaactaaa catcgcggta
     1141 atcgatgcgc ttgccagaga gtggaaatga acgttaaacc cgaccatcgc gccgctggct
     1201 ccttcatcga catcaatacg ctctacatcc agcgcgtgaa cggtaaaaat gtagcgatgg
     1261 gtttcgcctt tcggcggcgc tgcgccatcg tatccggttt taccaaaatc ggtacgcgtc
     1321 tgcaaaacgc cgtctggcat tgctaccaga ccagagccaa acccttgcgg taatacgcgg
     1381 gtatcagcgg gtaaattaac aactacccag tgccaccagc cggagccggt tggcgcatcc
     1441 gggtcgtagc aggtgacaac aaaacttttc gttcccgcag gaacatcatc ccacgccaga
     1501 tgcggtgaaa tattatcgcc atcgtaaccc atgccgttaa agacatgacg atgcggcaac
     1561 ttatcgccat cgcgcagatc gttactgatg agtttcatga accctccttt cttgtttgca
     1621 gaaagtgtag ccagaaaccc tcacgctgac ttcccgttat tggcaaaaaa atgtttcatc
     1681 ctgtaccgcg cggttaaccg ctgcggtcag acgctgcaac tgttgcggga gaataatata
     1741 gggcggcatc aggtaaatca gtttgccaaa aggccggatc cataccccct gttcgacaaa
     1801 gaatttttgc agcgccgcca tattcaccgg acgagtggtt tcgaccacac cgattgcccc
     1861 cagtacgcgc acatcggcaa ccatttcggc atcacgggct ggtgccagtt gctcgcgcag
     1921 ctgcacttca atagccgcca cctgctgctg ccattcgccg gattcgataa tcgctaggct
     1981 ggcgtttgct gccgcgcagg ccagcggatt gcccataaaa gttggcccat gcataaagca
     2041 gccggcttcg ccgttactga tggtttctgc aacctcgcgc gtggtaagtg tggcggaaag
     2101 ggtcattgtg ccgccggtta aggctttacc gaggcacaaa atgtccggcg cgatttctgc
     2161 atattcacag gcaaacagtt tgccggtacg accaaatccg gtggcgatct cgtcggcaat
     2221 cagcaagata ccttcgcgat cgcacatttt gcggattcgt tttaaccatt ccggatggta
     2281 catgcgcatc ccgcctgcgc cctggacaat cggctcaatg atcaccgctg cgatttcatg
     2341 acgatgcgcc gccatcaggc gggcaaaacc aaccatatcg cgttcatccc attcaccatc
     2401 catgcggctt tgcggggcgg gagcaaacaa gttttctggc agatagcctt tccacaggct
     2461 gtgcattgag ttgtccggat cgcacaccga catcgcgcca aaggtatcgc catgataacc
     2521 attgcggaag gtcagaaaac gctggcgcgc ttcgcctttg gcttgccagt actgcaacgc
     2581 cattttcatc gccacttcca ccgctacgga accggagtcc gcgagaaaaa cgcactccag
     2641 cggttgcggc gtcattgcca ccagtttgcg gcatagctcg atggcgggcg catgggtgat
     2701 accgccaaac atcacatgcg acatggcatc aatttgcgtc ttcatcgccg cattaagctg
     2761 cgggtgattg tagccgtgga tcgccgccca ccaggacgac ataccgtcaa ccaggcgtct
     2821 gccgtcagac aaaattagct cgcaaccttc ggcgctcgcc accggataaa ccggcagagg
     2881 ggaggtcatg gatgtgtatg ggtgccagat atggcgttgg tcaaaggcaa gatcgtccgt
     2941 tgtcataatc gacttgtaaa ccaaattgaa aagatttagg tttacaagcc tacaccgaat
     3001 taacaacaaa aaacacgttt tggagaagcc ccatggctca ccgcccacgc tggacattgt
     3061 cgcaagtcac agaattattt gaaaaaccgt tgctggatct gctgtttgaa gcgcagcagg
     3121 tgcatcgcca gcatttcgaa cctcgtcagg tgcaggtcag cacgttgctg tcgattaaga
     3181 ccggagcttg tccggaagat tgcaaatact gcccgcaaag ctcgcgctac aaaaccgggc
     3241 tggaagccga gcggttgatg gaagttgaac aggtgctgga gtcggcgcgc aaagcgaaag
     3301 cggcaggatc gacgcgcttc tgtatgggcg cggcatggaa gaatccccac gaacgcgata
     3361 tgccgtacct ggaacaaatg gtgcaggggg taaaagcgat ggggctggag gcgtgtatga
     3421 cgctgggcac gttgagtgaa tctcaggcgc agcgcctcgc gaacgccggg ctggattact
     3481 acaaccacaa cctggacacc tcgccggagt tttacggcaa tatcatcacc acacgcactt
     3541 atcaggaacg cctcgatacg ctggaaaaag tgcgcgatgc cgggatcaaa gtctgctcag
     3601 gtggcattgt gggcttaggc gaaacggtaa aagatcgcgc cggattattg ctgcaactgg
     3661 caaacctgcc gacgccgccg gaaagcgtac caatcaacat gctggtgaag gtgaaaggca
     3721 cgccgcttgc cgataacgat gacgttgatg cctttgattt tattcgcacc attgccgtag
     3781 cgcggatcat gatgccgacc tcttacgtgc gcctttctgc cggacgcgag cagatgaacg
     3841 aacagactca ggcgatgtgc tttatggcag gcgcaaactc gattttctac ggttgcaaac
     3901 tgctgaccac gccgaatccg gaagaagata aagacctgca actgttccgc aaactggggc
     3961 taaatccgca gcaaactgcc gtgctggcag gggataacga acaacagcaa cgtcttggac
     4021 aggcgctgat gaccccggac accgacgaat attacaacgc ggcagcatta tgagctggca
     4081 ggagaaaatc aacgcggcgc tcgatgcgcg gcgtgctgcc gatgccctgc gtcgccgtta
     4141 tccggtggcg caaggagccg gacgctggct ggtggcggat gatcgccagt atctgaactt
     4201 ttccagtaac gattatttag gtttaagcca tcatccgcaa attatccgtg cctggaagca
     4261 gggggcggag caatttggca tcggtagcgg cggctccggt cacgtcagcg gttatagcgt
     4321 ggtgcatcag gcgctggaag aagaactggc cgagtggctt ggctattcgc gggcgctgct
     4381 gtttatctct ggttttgccg cgaatcaggc agtcattgcc gcgatgatgg cgaaagagga
     4441 ccgtattgtt gccgaccggc ttagccatgc ctcattgctg gaggctgcca gtttaagccc
     4501 gtcgcagctt cgccgtttta ctcataacga tgtcgctcat ctggcgcgac tgcttgcttc
     4561 cccctgtccg gggcagcaac tggtggtgac agaaggcgtg ttcagcatgg acggcgatag
     4621 cgcgccactg gcggaaatcc agcaggtaac gcaacagcac aatggctggt tgatggtcga
     4681 tgatgcccac ggcacgggcg ttatcgggga gcaggggcgc ggcagctgct ggctgcaaaa
     4741 ggtaaaacca gaattgctgg tagtgacttt tggcaaagga tttggcgtca gtggggctgc
     4801 ggtgctttgc tcaagtacgg tggcggatta tctgctgcaa ttcgcccgcc accttatcta
     4861 cagcaccagt atgccgcccg ctcaggcgca ggcattacgt gcgtcgctgg cggtcattcg
     4921 ccgtgatgag ggggatgcac ggcgcgaaaa actggtgtca ctcattgcgc gttttcgtgc
     4981 cggagtacag gatttgccgt ttacgctggc tgattcatgc agcgccatcc agccattgat
     5041 cgtcggtgat aacagccgtg cgttacaact ggcagaaaaa ctgcgccagc aaggctgctg
     5101 ggtcacggcg attcgcccgc caaccgtacc cgctggtact gcgcgactgc gcttaacgct
     5161 aaccgctgcg catgaaatgc aggatatcga ccgtctgctg gaggtgctgc atggcaacgg
     5221 ttaataaaca agccattgca gcggcatttg gtcgggcagc cgcacactat gagcaacatg
     5281 cagatctaca gcgccagagt gctgacgcct tactggcaat gcttccacag cgtaaataca
     5341 cccacgtact ggacgcgggt tgtggacctg gctggatgag ccgccactgg cgggaacgtc
     5401 acgcgcaggt gacggcgtta gatctctcgc cgccaatgct tgcgcaagcc cgccataaag
     5461 atgccgcaga tcattatctg gcaggagata tcgaatccct gccgttagcg actgcgacgt
     5521 tcgatcttgc atggagcaat ctcgcggtgc agtggtgcgg taatttatcc acggcactcc
     5581 gcgagctgta tcgggtggtg cgccccggag gcgtggtcgc gtttaccacg ctggtgcagg
     5641 gatcgttacc cgaactgcat caggcgcggc aggcggtgga cgagcgtccg catgctaatc
     5701 gctttttacc gccagatgaa atcgaagagt cgctgaacgg cgtgcgttat caacatcata
     5761 ttcagctcat cacactatgg tttgatgatg cgctcagtgc catgcgttcg ctgaaaggca
     5821 tcggtgctac gcatcttcat gaagggcgcg acccgcgaat attaacgcgt tcgcagttgc
     5881 agcgattgca actggcctgg ccgcaacaac aggggcgata tcctctgacg tatcatcttt
     5941 ttttgggagt gattgctcgt gagtaaacgt tattttgtca ccggaacgga taccgaagtg
     6001 gggaaaaccg tcgccagttg tgcactttta caagccgcga aggcagtagg ttatcggacg
     6061 gcaggttata aaccggtcgc ctctggcagc gaaaagaccc cggaaggctt acgtaatagt
     6121 gacgcgctgg cgttacagca caacagcagc ctgcagctgg attacgcaac ggtaaatcct
     6181 tacaccttcg cagaatccac ctcgccgcac atcatcagcg cccaagaggg caggccgata
     6241 gaatcattgg taatgagcgc cggattacgc gcgcttgaac aacaggctga ctgggtgtta
     6301 gtggaaggtg ctggcggctg gtttacgccg ctttctgaca ctttcacttt tgcagattgg
     6361 gtaacacagg aacaactgcc ggtgatactg gtagttggtg tgaaactcgg ctgtattaat
     6421 cacgcgatgt tgactgcgca ggcaatacaa cacgccggac tgactctggc gggttgggtg
     6481 gcgaacgatg ttacgcctcc gggaaaacgt cacgctgaat atatgaccac gctcacccgc
     6541 atgattcccg cgccgctgct gggagagatc ccctggcttg cagaaaatcc agaaaatgcg
     6601 gcaaccggaa agtacataaa ccttgccttg ttgtagccat tctgtatttg gttaaattgc
     6661 gagcgagatc gcgtcttcga ttgactgcaa tttaaccaat taaattctaa aataatcacg
     6721 aaaaaaattt tacttccgcc tcatgcggcg aatgtgggaa ttgcccaggc ggcgggggat
     6781 aggggctgga gacagttatc cactattcct gtggataacc atgtgtatta gagttagaaa
     6841 acacgaggca agcgagagaa tacgcggctt gcacgcgaat tggcgttaaa gacggctcaa
     6901 agaaatatct tttatttttt aactggttag ataaatgcaa tggcagtcac tgaacaggca
     6961 tctcttgcca taaaactgtc atcactcatc ttgacaaatg ttaaaaaagc cgttgctttg
     7021 gggataaccc ggtaaggctg gagttttatc tcgccacaga gtaaattttg ctcatgattg
     7081 acagcggagt ttacgctgta tcagaaatat tatggtgatg aactgttttt ttatccagta
     7141 taatttgttg gcataattaa gtacgacgag taaaattaca tacctgcccg cccaactcct
     7201 tcaggtagcg actcatgagt aaaccgttca aactgaattc cgcttttaaa ccttctggcg
     7261 atcagccaga ggcgattcga cgtctcgaag aggggctgga agatggcctg gcgcaccaga
     7321 cgttacttgg cgtgactggc tccgggaaaa ccttcaccat tgccaatgtc attgctgacc
     7381 ttcagcgccc aaccatggta cttgcgccca acaaaacgct ggcggcccag ctgtatggcg
     7441 aaatgaaaga gttcttcccg gaaaacgcgg tggagtattt cgtctcctac tacgactact
     7501 atcagccgga agcctatgta ccgagttccg acaccttcat tgagaaagat gcctcggtta
     7561 acgaacatat tgagcagatg cgtttgtccg ccaccaaagc gatgctggag cggcgtgatg
     7621 tgattgtagt ggcgtctgtt tccgcgattt atggtctggg cgatcctgat ttatatctca
     7681 agatgatgct ccatctcacg gtcggtatga ttatcgatca gcgcgcgatt ctgcgccgac
     7741 tggcggagct gcaatacgct cgtaatgatc aagcattcca gcgtggtact ttccgcgttc
     7801 gtggcgaggt gatagatatc ttcccggcag aatcggatga cattgcactt cgcgtggaac
     7861 tgtttgacga ggaagtggaa cgattgtcgt tatttgaccc gctgaccggg cagattgttt
     7921 ccactattcc acgttttacc atctacccga aaacgcacta cgtcacaccg cgcgagcgca
     7981 tcgtacaggc gatggaggag atcaaagaag aactggccgc cagacgcaaa gtgctgttgg
     8041 aaaacaacaa actgctggaa gagcagcggc tgacccagcg tacccagttt gatctggaga
     8101 tgatggacga gctgggctac tgttcgggga ttgaaaacta ctcgcgcttc ctctccggtc
     8161 gtggaccggg tgagccaccg ccgacgctgt ttgattacct gcctgccgat gggctgctgg
     8221 tcgtcgatga atctcacgtc accattccac aaattggcgg catgtatcgc ggtgaccggg
     8281 cgcgtaaaga gacactggtg gagtacggct tccgcctgcc atcagcgctg gataaccgtc
     8341 cgcttaagtt tgaagagttc gaagcattag cgccgcaaac catctatgtt tcggcgacgc
     8401 cgggtaatta cgagctggaa aaatccggcg gcgatgtggt ggatcaggtg gtgcgtccaa
     8461 ccggattgct tgacccgatt atcgaagtgc ggccggtggc gacacaggtt gatgatcttc
     8521 tttcggagat tcgtcagcga gcggcaatta acgaacgcgt gctggtcacc acactgacca
     8581 agcgaatggc ggaagatctt accgaatatc tcgaagaaca cggtgagcgc gtgcgttatc
     8641 ttcactcaga tatcgacacc gtcgagcgta tggagattat ccgcgacttg cgtctgggtg
     8701 agttcgacgt attggtaggg atcaacttac tgcgtgaagg tctggatatg ccggaagtgt
     8761 cgctggtggc gatcctcgac gctgacaaag aaggcttcct gcgttccgaa cgttcgttga
     8821 tccagaccat tggtcgtgcg gcacgtaacg ttaacggtaa agcgattctc tacggcgata
     8881 ggagcactcc atcaatggcg aaagcgattg gcgaaaccga acgtcgccgt gagaaacagc
     8941 agaagtacaa cgaggaacac ggcattacgc cgcaaggctt gaacaagaaa gtggtcgata
     9001 tcctggcgct ggggcagaac attgccaaaa ccaaagcgaa gggcagagga aaatcgcgcc
     9061 cgattgttga gccggataat gtgccgatgg atatgttgcc gaaagcgttg cagcagaaaa
     9121 tccatgaact ggaagggctg atgatgcaac acgcgcagaa tctggagttc gaagaagcgg
     9181 cacaaattcg tgaccagttg catcagttgc gtgagttgtt tattgccgca tcgtaacagg
     9241 atagcgaaga agactgatga caaacggaaa acagtctgat gcgctacgct tatcaggcct
     9301 acattttctc cgcaatatat tgaatttgcg cggtttgtag gccggataag gcgttcacgc
     9361 cgcatccggc ataaacaacg cgcactttgt cttcaatagg aagccggaat tttccttcgg
     9421 atttccgtta acctaaagcc tgtaacgcct tttccagcgc gttatgtaac aactggcggt
     9481 catgacgata cggaatatcg ctggcctcca gtacctcctg gatcacaatc cgctctttca
     9541 ccgccgagac atccactttt ggcccgacga tgaccgcatc aatgaccttt ttaccaacat
     9601 actgctccat aattgccagc ttgctttcca gcttcaaatt agccgcaggt aaactcaact
     9661 cacgccccag attgccgata taaaccatcg gcgctggcgt gcggcgtaat gcctgggcga
     9721 tttccttcag cagcagaatt ggcatcaggc tggtataaaa actgccaggc ccaataatga
     9781 tgagatccgc ttcattgata gcgtgaaccg cctcacgcgt tgcgggtaca ttaggcgtta
     9841 acaataactc ttgaatcggc gtagttaact ggtcgatatt gacctcgccg taaacttcat
     9901 gcccctgatc gtcaatcgcc atcagatcaa caggatgctc tgacattgga atcaaatgcg
     9961 tatccacttt cagcagatta cgaattaaat tgatggcttc cagaggccgc acgctaaggt
    10021 gatccagcgc ctttaacatc aagtttccga gattatgacc ggaaagttcg ccattgccgc
    10081 caaaacggta ttcaaacatc gcggaggcga cgttcggttc cgttatcagc tggttgaggc
    10141 agttgcgcat atcgccccaa gcaatgccgc cttctgaacg gcgaatacgc cccgtcgagc
    10201 caccattatc cgtggtggtg acgatacccg ttaaacgaga acccaaagac gaaagtgatg
    10261 agagaacgcg tcccagtcca tgccctccgc cgagagcaac gacacgatca agatcagcca
    10321 gcgtacgatt gcgcatatag tttcctggag tcagattatc cgcgctacag tagcgcaaat
    10381 tgcggggaaa cagcaattaa cctgccaata ttgaggatac aaactatttt cttctatctc
    10441 taaaggacga tgcacgctat gcctccctga tgatgtatat caaagtaaaa ccgccatttt
    10501 cccttattct gtagcgaaat agcacgatca tgacgctata tacatgatta catagcgaaa
    10561 gtgtggatgg taaaaatctc atttacacgc tagtatcggc ttaaccacta aacactctag
    10621 cctctgcacc tgggtcaact gatacggtgc tttggccgtg acaatgctcg taaagattgc
    10681 caccagggcg aaggaagaaa tgacttcgcc tcccgtatct ggaaaggtgt acatggcttc
    10741 acaactgact gatgtatttg cgcgtaagtt ttactacttg cgcctgtcga ttaccgatgt
    10801 gtgtaacttt cgttgcacct actgcctgcc ggatggctac aaaccgagcg gcgtcaccaa
    10861 taaaggcttt cttaccgtcg atgaaattcg ccgggttacg cgcgccttcg ccagtctggg
    10921 caccgaaaaa gtgcgcctga caggaggaga gccgtcttta cgccgcgact ttaccgatat
    10981 catcgccgct gtgcgggaaa acgacgctat ccgccagatt gcggtcacca ccaatggtta
    11041 ccgtctggaa cgcgatgtgg cgaactggcg cgatgcggga cttactggca ttaacgtcag
    11101 tgtcgacagt ttggacgccc gccagtttca cgctattacc gggcaggata aattcaacca
    11161 ggtcatggca gggattgatg ctgcatttga ggccggtttt gagaaggtca aagtcaatac
    11221 cgtgctgatg cgtgatgtta atcatcacca gctcgacacc tttctgaact ggatccagca
    11281 tcgccctatc cagctgcgtt tcatcgaact gatggaaacg ggcgagggca gcgagctctt
    11341 ccgtaagcat cacatctctg gtcaggttct gcgtgacgag ctactgcgtc gcggctggat
    11401 ccaccaatta cgtcaacgca gcgacggtcc cgcgcaagtc ttttgccatc cagattacgc
    11461 cggagagatt ggccttatca tgccgtatga aaaagacttc tgcgccactt gcaaccgcct
    11521 gcgcgtttcc tccattggta aactccatct ctgcctgttt ggtgaaggcg gcgttaacct
    11581 gcgcgatctg ctggaaggcg atacccagca acaggcgctg gaagcgcgta tttcagcggc
    11641 gctgcgggag aagaaacaga cccatttcct gcatcaaaac aacaccggta ttacgcaaaa
    11701 cttatcgtac attggcggct aaaacgtcaa aaggagagat cagatgagtc aggtaagcac
    11761 tgaatttatc ccgacccgta ttgctattct tacggtttct aatcgtcgcg gtgaagaaga
    11821 cgatacctcc ggtcactatc tgcgcgattc ggcgcaagaa gcgggccatc acgttgtcga
    11881 tgaagccatt gtgaaagaaa accgctacgc tattcgcgct caggtatctg cgtggatcgc
    11941 cagcgacgat gtacaagtgg tattgattac gggtggtact ggcctgacgg aaggtgatca
    12001 ggctcccgaa gcgctgctgc cgttgttcga ccgtgaggtt gaaggttttg gtgaagtgtt
    12061 ccgtatgttg tcgtttgaag agattggcac ttccacgttg caatctcgtg cggtagcggg
    12121 cgtcgctaac aaaacgctga ttttcgccat gccgggttcg accaaagcgt gccgtaccgc
    12181 atgggaaaat atcatcgcgc cgcagctgga tgcccgtacg cgtccgtgta atttccatcc
    12241 acatttgaag aaataagtat gtcgcaactg acccatatca acgccgctgg cgaagcgcac
    12301 atggtggatg tctccgccaa agcggaaacc gtgcgtgaag cgcgggcgga agcctttgtc
    12361 accatgcgca gcgagacgct ggcgatgatt attgatggtc gccaccacaa aggcgacgta
    12421 tttgccactg cgcgtattgc cggtattcag gcggcaaaac gcacttggga tctgatcccg
    12481 ctgtgtcacc cgctgatgct cagcaaagtt gaagtcaatt tacaggccga gccggagcac
    12541 aatcgggtgc gtatagaaac cttatgccgc ctgaccggga aaaccggtgt cgaaatggaa
    12601 gcgttaaccg cggcctccgt ggcggcgctg accatttatg acatgtgcaa agcggtgcaa
    12661 aaagatatgg tgattggtcc ggtacgtttg ctggcgaaga gcggcggcaa gtcgggtgac
    12721 tttaaggtgg aagcggatga ttaaagttct ttttttcgcc caggtgcgcg agttggtggg
    12781 aacagatgca accgaagtgg ctgcggatta cccaaccgtt gaagcgttac gccagcacct
    12841 ggctgcgcag agcgatcgct gggcgctggc gctggaagat ggcaaattgc tggctgccgt
    12901 caaccagacg ctggtgagtt ttgaccatcc gctgactgac ggcgacgaag tagctttctt
    12961 cccgccggta accggaggtt aagatggcag aaaccaaaat tgttgtcggc ccgcagccgt
    13021 tcagcgtagg agaagagtac ccgtggctgg cggagcgtga cgaagacggt gcggtagtca
    13081 ccttcaccgg aaaggtgcgc aaccataacc tgggcgacag cgtcaaagca ttaaccctcg
    13141 aacactatcc ggggatgact gaaaaagcac tggcagaaat tgttgatgaa gcgcgtaacc
    13201 gctggccgct ggggcgcgtc acggtgattc accgcatcgg ggaattatgg ccgggcgatg
    13261 aaatcgtttt tgtcggtgtc accagtgcgc atcgcagcag tgcgtttgaa gccgggcagt
    13321 ttattatgga ttatctcaaa acccgcgcac cgttctggaa gcgcgaagcc acgccggaag
    13381 gcgaccgctg ggttgaagcc cgggagagcg atctgcaggc ggcaaaacgc tggtagtttt
    13441 ttgttagccg gataaggcac cgctgcgtcc ggcaatttca ccgctcagta catgcgttta
    13501 cgctgcgtcg atgcacagcc tcatcatttt gcagtatcct taagatattc cttatatctt
    13561 caggagatcg tcatggacag attcccacgt tctgattcaa tcgtacaacc ctgggctggc
    13621 ttgcaaactt atatggcgca agtctatggc tggatgaccg ttggcttgtt gctgaccgca
    13681 tttgttgcct ggtatgcggc taattccgcg gccgtgatgg agctgttgtt cactaaccgt
    13741 gtctttttaa tcggtctgat catcgcgcaa ttagcattgg ttattgtgtt atcagcgatg
    13801 attcaaaagc tgagcgcagg tgtaacgacg atgctcttta tgctttattc ggcgctgacg
    13861 ggtcttacgc tttccagtat attcattgtc tataccgctg cttctatcgc cagtactttc
    13921 gtcgttactg ccgggatgtt cggcgcaatg agcctgtacg gttacaccac gaagcgcgat
    13981 ttaagtggct ttggcaatat gctgtttatg gcgttaatcg gcattgtgct ggcatcgctg
    14041 gtcaacttct ggctgaaaag cgaagcattg atgtgggcag ttacctacat cggcgtgatt
    14101 gtctttgtcg gattgacggc gtatgacact cagaaattga aaaatatggg tgagcagatt
    14161 gatacccgcg acacgtcgaa cctgcgcaaa tattccattc ttggtgcgtt aaccttgtat
    14221 ctggacttca tcaacctgtt cctgatgttg ttacggatct tcggcaaccg ccgttaattc
    14281 tttttcacca tcactatccc ggccatgtgt cgggattttt ttcttactaa aaacgacact
    14341 taacaagtac caggtataaa atatgcctta tgagcgagcg gaatcgtttt ccgtatccat
    14401 aaatacaaaa gtattttgta tgcgtggttt taaatttatt gatggtgaat attaatgttt
    14461 tttctaagga caactaaaag aatggaatca tactcgcaaa acagcaataa attagatttt
    14521 cagcacgagg ccaggatatt aaacggtata tggctcatta ccgctttagg tttggtggca
    14581 accgcaggac tagcctgggg agctaagtat ctcgaaatta cggcaaccaa atatgattca
    14641 ccaccaatgt atgtcgccat agggttatta ttactttgta tgtatggctt aagtaaggat
    14701 atcaacaaga taaatgccgc catcgcgggc gtaatatatc tgtttttact ctctttggtg
    14761 gcgattgtcg ttgcaagttt agttcctgta tatgccatta tcatcgtgtt cagcactgcg
    14821 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
    14881 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
    14941 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
    15001 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
    15061 gagttgtgga gtccggttcg ttgcgctttt acaggtgcat taacactcta ttactatttt
    15121 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
    15181 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
    15241 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
    15301 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
    15361 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
    15421 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
    15481 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
    15541 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
    15601 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
    15661 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
    15721 taatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
    15781 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
    15841 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
    15901 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
    15961 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
    16021 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
    16081 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
    16141 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag gggtttaccc
    16201 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
    16261 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
    16321 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
    16381 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
    16441 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
    16501 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
    16561 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
    16621 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
    16681 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
    16741 agtaggcgtt ggcgataatc acttcccgct gcgcctgagt gagcattttc agataatggc
    16801 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
    16861 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
    16921 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
    16981 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
    17041 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
    17101 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
    17161 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
    17221 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
    17281 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
    17341 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
    17401 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
    17461 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
    17521 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
    17581 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
    17641 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
    17701 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
    17761 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
    17821 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
    17881 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
    17941 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
    18001 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
    18061 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
    18121 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
    18181 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
    18241 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
    18301 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
    18361 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
    18421 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
    18481 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
    18541 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
    18601 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
    18661 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
    18721 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
    18781 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
    18841 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
    18901 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
    18961 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
    19021 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
    19081 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
    19141 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
    19201 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
    19261 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
    19321 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
    19381 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
    19441 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
    19501 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
    19561 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
    19621 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
    19681 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
    19741 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
    19801 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
    19861 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
    19921 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
    19981 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
    20041 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
    20101 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
    20161 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
    20221 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
    20281 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
    20341 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
    20401 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
    20461 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
    20521 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
    20581 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
    20641 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
    20701 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
    20761 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
    20821 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
    20881 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
    20941 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
    21001 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
    21061 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
    21121 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
    21181 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
    21241 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
    21301 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
    21361 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
    21421 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
    21481 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
    21541 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
    21601 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
    21661 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
    21721 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
    21781 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
    21841 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgcca
    21901 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
    21961 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
    22021 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
    22081 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
    22141 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
    22201 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
    22261 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
    22321 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
    22381 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
    22441 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
    22501 agtaatcccg ccaacatccg catcagtgtg gttttacctg caccgtccgg ccccaccaac
    22561 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
    22621 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
    22681 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
    22741 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
    22801 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
    22861 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
    22921 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
    22981 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
    23041 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
    23101 gctggctttc gcctgagcga tgtcctgttc acggttaccg gaacggtact gacgcaattt
    23161 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
    23221 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
    23281 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
    23341 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
    23401 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
    23461 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
    23521 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
    23581 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
    23641 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
    23701 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
    23761 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
    23821 gcatgggtat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
    23881 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
    23941 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
    24001 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
    24061 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
    24121 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
    24181 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
    24241 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
    24301 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
    24361 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
    24421 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
    24481 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
    24541 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
    24601 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
    24661 ctgtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
    24721 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
    24781 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    24841 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    24901 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    24961 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    25021 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    25081 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    25141 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    25201 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    25261 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    25321 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    25381 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    25441 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    25501 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    25561 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    25621 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    25681 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    25741 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    25801 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    25861 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    25921 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    25981 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    26041 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    26101 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    26161 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    26221 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    26281 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    26341 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    26401 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    26461 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    26521 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    26581 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    26641 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    26701 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    26761 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    26821 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    26881 ttcactgccg ctttcgggcg tgggcgctac gtttgtccac gtaatctgac ggcgctcgcc
    26941 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    27001 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    27061 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    27121 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    27181 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    27241 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    27301 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    27361 gtgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    27421 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    27481 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    27541 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    27601 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    27661 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    27721 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    27781 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    27841 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    27901 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    27961 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    28021 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    28081 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    28141 gtggagagca aaaaatatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    28201 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    28261 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    28321 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    28381 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    28441 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    28501 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    28561 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    28621 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    28681 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    28741 tatcgcaaaa tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    28801 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    28861 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    28921 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    28981 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    29041 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    29101 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    29161 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    29221 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    29281 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    29341 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    29401 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    29461 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    29521 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    29581 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    29641 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    29701 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    29761 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    29821 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    29881 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    29941 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    30001 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    30061 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    30121 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    30181 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    30241 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    30301 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    30361 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    30421 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    30481 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    30541 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    30601 cgacgcatca ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    30661 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    30721 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    30781 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    30841 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    30901 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    30961 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    31021 cttgtgctgg acgtagcgta aacgcctgat tcgtccaacg gttcggattt tgtaggcctg
    31081 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    31141 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    31201 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    31261 agggcatcga ggggggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    31321 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    31381 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    31441 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    31501 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat
    31561 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    31621 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    31681 cgtaactgtc tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa
    31741 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    31801 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    31861 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    31921 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    31981 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    32041 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    32101 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    32161 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    32221 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    32281 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    32341 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    32401 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    32461 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    32521 attttgcagt gcggcgtata acgtgctgcg ggtgtctact tgctgattgt tcttaacttg
    32581 tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
    32641 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    32701 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    32761 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    32821 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    32881 taccccagtt tggcatcggc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    32941 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    33001 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    33061 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag
    33121 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    33181 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    33241 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    33301 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    33361 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    33421 tagttaatat agacattgcc gttttccgtc aggtggtaca gcgccccggc tttccagttc
    33481 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    33541 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
    33601 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    33661 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    33721 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    33781 accggattaa cgccatagtt agtctgcgtt tcacgggtaa attccacgcc ggtact
//
LOCUS       DAJTYL010000035        33810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYL010000035 DAJTYL010000000
VERSION     DAJTYL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.035"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..107)
                     /locus_tag="OO572_001601"
     CDS             complement(<1..107)
                     /locus_tag="OO572_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6660644.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLIT"
     gene            392..1618
                     /gene="mdfA"
                     /locus_tag="OO572_001602"
     CDS             392..1618
                     /gene="mdfA"
                     /locus_tag="OO572_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6660645.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(1659..1943)
                     /locus_tag="OO572_001603"
     CDS             complement(1659..1943)
                     /locus_tag="OO572_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660646.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(2029..2844)
                     /gene="ybjI"
                     /locus_tag="OO572_001604"
     CDS             complement(2029..2844)
                     /gene="ybjI"
                     /locus_tag="OO572_001604"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6660647.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(2844..4052)
                     /locus_tag="OO572_001605"
     CDS             complement(2844..4052)
                     /locus_tag="OO572_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6660648.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4136..4672
                     /gene="rcdA"
                     /locus_tag="OO572_001606"
     CDS             4136..4672
                     /gene="rcdA"
                     /locus_tag="OO572_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6660649.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(4847..6532)
                     /locus_tag="OO572_001607"
     CDS             complement(4847..6532)
                     /locus_tag="OO572_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6660650.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            6802..7179
                     /locus_tag="OO572_001608"
     CDS             6802..7179
                     /locus_tag="OO572_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6660651.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(7209..7466)
                     /gene="grxA"
                     /locus_tag="OO572_001609"
     CDS             complement(7209..7466)
                     /gene="grxA"
                     /locus_tag="OO572_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6660652.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            7626..7913
                     /locus_tag="OO572_001610"
     CDS             7626..7913
                     /locus_tag="OO572_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6660653.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            7897..8619
                     /gene="nfsA"
                     /locus_tag="OO572_001611"
     CDS             7897..8619
                     /gene="nfsA"
                     /locus_tag="OO572_001611"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6660654.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            8680..9582
                     /gene="rimK"
                     /locus_tag="OO572_001612"
     CDS             8680..9582
                     /gene="rimK"
                     /locus_tag="OO572_001612"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6660655.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            9670..10146
                     /locus_tag="OO572_001613"
     CDS             9670..10146
                     /locus_tag="OO572_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6660656.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            10498..11610
                     /gene="potF"
                     /locus_tag="OO572_001614"
     CDS             10498..11610
                     /gene="potF"
                     /locus_tag="OO572_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6660657.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            11705..12838
                     /gene="potG"
                     /locus_tag="OO572_001615"
     CDS             11705..12838
                     /gene="potG"
                     /locus_tag="OO572_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6660658.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            12848..13801
                     /gene="potH"
                     /locus_tag="OO572_001616"
     CDS             12848..13801
                     /gene="potH"
                     /locus_tag="OO572_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6660659.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            13798..14643
                     /gene="potI"
                     /locus_tag="OO572_001617"
     CDS             13798..14643
                     /gene="potI"
                     /locus_tag="OO572_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6660660.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            14703..15191
                     /locus_tag="OO572_001618"
     CDS             14703..15191
                     /locus_tag="OO572_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6660661.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            15232..16359
                     /gene="rlmC"
                     /locus_tag="OO572_001619"
     CDS             15232..16359
                     /gene="rlmC"
                     /locus_tag="OO572_001619"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6660662.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16534..17265)
                     /gene="artJ"
                     /locus_tag="OO572_001620"
     CDS             complement(16534..17265)
                     /gene="artJ"
                     /locus_tag="OO572_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6660663.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17557..18225)
                     /gene="artM"
                     /locus_tag="OO572_001621"
     CDS             complement(17557..18225)
                     /gene="artM"
                     /locus_tag="OO572_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6660664.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(18225..18941)
                     /gene="artQ"
                     /locus_tag="OO572_001622"
     CDS             complement(18225..18941)
                     /gene="artQ"
                     /locus_tag="OO572_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6660665.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(18948..19679)
                     /gene="artJ"
                     /locus_tag="OO572_001623"
     CDS             complement(18948..19679)
                     /gene="artJ"
                     /locus_tag="OO572_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6660666.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEIITVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(19697..20425)
                     /gene="artP"
                     /locus_tag="OO572_001624"
     CDS             complement(19697..20425)
                     /gene="artP"
                     /locus_tag="OO572_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6660667.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(20643..21158)
                     /locus_tag="OO572_001625"
     CDS             complement(20643..21158)
                     /locus_tag="OO572_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6660668.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            21807..23576
                     /locus_tag="OO572_001626"
     CDS             21807..23576
                     /locus_tag="OO572_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6660669.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            23787..24110
                     /locus_tag="OO572_001627"
     CDS             23787..24110
                     /locus_tag="OO572_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6660670.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            24107..24937
                     /gene="amiD"
                     /locus_tag="OO572_001628"
     CDS             24107..24937
                     /gene="amiD"
                     /locus_tag="OO572_001628"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6660671.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(24934..25947)
                     /locus_tag="OO572_001629"
     CDS             complement(24934..25947)
                     /locus_tag="OO572_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6660672.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(26046..27476)
                     /locus_tag="OO572_001630"
     CDS             complement(26046..27476)
                     /locus_tag="OO572_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6660673.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(27487..28488)
                     /gene="ltaE"
                     /locus_tag="OO572_001631"
     CDS             complement(27487..28488)
                     /gene="ltaE"
                     /locus_tag="OO572_001631"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6660674.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(28525..30243)
                     /gene="poxB"
                     /locus_tag="OO572_001632"
     CDS             complement(28525..30243)
                     /gene="poxB"
                     /locus_tag="OO572_001632"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6660675.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(30376..31344)
                     /gene="hcr"
                     /locus_tag="OO572_001633"
     CDS             complement(30376..31344)
                     /gene="hcr"
                     /locus_tag="OO572_001633"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6660676.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(31356..33008)
                     /gene="hcp"
                     /locus_tag="OO572_001634"
                     /gene_synonym="priS"
     CDS             complement(31356..33008)
                     /gene="hcp"
                     /locus_tag="OO572_001634"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6660677.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(33152..>33810)
                     /gene="lysO"
                     /locus_tag="OO572_001635"
     CDS             complement(33152..>33810)
                     /gene="lysO"
                     /locus_tag="OO572_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6660678.1"
                     /translation="WLERGLPWRNHHQQEKLPSRIAMALESLKLCGVVVIGFAIGLSG
                     LAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVVVVVSSLIGGL
                     INAFILDLPINTALAMASGFGWYSLSGILLTESFGPVIGSAAFFNDLARELIAIMLIP
                     GLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMVPAAIVHGFILSLLVPILIAFFSA
                     "
BASE COUNT         8354 a         8356 c         8704 g         8396 t
ORIGIN      
        1 gtaatcaaat ctttagcaat aaaaatcgcc aacgagatca tccacggagc cgagtctggc
       61 gtcgcgttaa taagagagaa tagagagaga ttcaaatttt ccagcataac ttcccgacgc
      121 aaagtgatta aaagggagcc aatacaggca agtcgttgag aataaagtgc aggttaaact
      181 gggtaaagcg gcatcgtctt atttccctca agcggcctgt ttacggtggg tgattgtaac
      241 gggcataggt taaataaaac ttaaagaaag cgtagctata ctcgcaataa tgtaagaatg
      301 tgcttaaccg tggtttcagc tacaaaattc gctttctcgt tagctgcgct tttattaaac
      361 tctgcgcgat tattattggc gaagaaattg catgcaaaat aaattagctt ccggtgccag
      421 gcttggacgt caggcgttac ttttccctct ctgtctggtg ctttacgaat tttcaaccta
      481 tatcggcaac gatatgatcc aacccggtat gttggccgtg gtggaacaat atcaggcggg
      541 cattgattgg gttcctactt cgatgaccgc gtatctggcg ggcgggatgt ttttacaatg
      601 gctcctgggg ccgctgtcgg atcgtattgg tcgccgtccg gtgatgctgg cgggtgtggt
      661 gtggtttatc gtcacctgtc tggcaatatt actggcgcaa aacattgaac aattcaccct
      721 gttgcgcttc ttgcagggca taagcttctg tttcattggc gctgtgggat acgccgcaat
      781 tcaggaatcc ttcgaagagg cggtttgtat caagatcacc gcgctgatgg cgaacgtggc
      841 gctgattgct ccgctacttg gtccgctggt gggcgcggcg tggatccatg tgctgccttg
      901 ggaggggatg tttgttttgt ttgccgcatt ggcagcgatc tcctttttcg gtctgcaacg
      961 agctatgcct gaaaccgcca tgcgtatagg cgagaaactg tcactgaaag aactcggtcg
     1021 tgactataag ctggtgctga agaacggccg ctttgtggcg ggggcgctgg gattcgttag
     1081 cctgccattg ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt
     1141 gagcagctat gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa
     1201 cttgctgtta gcgcgtctga cctcgcgccg caccgtacgt tcgctgatta ttatgggcgg
     1261 ctggccgatt atgattggtc tgttggtcgc tgctgcggca acggttatct catcgcacgc
     1321 gtatttatgg atgaccgccg ggttaagtat ttatgctttc ggtattggtc tggcgaatgc
     1381 gggactggtg cgattaaccc tgtttgccag cgatatgagt aaaggtacgg tttctgcggc
     1441 gatgggaatg ctgcaaatgc tgatctttac cgtcggtatt gaaatcagca aacatgcctg
     1501 gctgaacggg ggcaacggac tgtttaatct cttcaacctt gtcaacggaa ttttgtggct
     1561 gtcgctgatg gttatctttt taaaagataa acagatggga aattctcacg aagggtaaaa
     1621 aaatgcctga ttgatttgtg cgatcaggca ttctcgaatt aatggtgatg gtcgtcaatc
     1681 tggtgttcga taaccatccc ttcacctacg ctggcaagat ggcgaacata aggatgcggg
     1741 cggtaagccg gagctggcgc aggagccaca tagacggttt gcggcacagt cgcgacgctg
     1801 accgcttgtg gaacgctgac tgaatcagga accacaacca ctttgtatcc actcggcaca
     1861 tcgacggtga cactttgcgc catcgccaca ccagtaaagc ccattaaaag cgcgcccaac
     1921 agtagacaat ttttcataat catttcgcct gaaataatgc agcgggttaa tttccgcgaa
     1981 ttatgcagcc ttcattcagg cattgtgttg ccgggcgtgc tgccgttttt attggtcaaa
     2041 tggcgcttcg tgtttaagaa ctttatcgat cacatccagt acgccttcac ggttattgta
     2101 gcctgcccgg tattttgccg ctgcgacgac tgcgctgccg gcattttcca ttgcaaaact
     2161 aaagcctgcc tgacgcagca tctcaatatc gttaccgcca tcgccaaaga ccaccacttc
     2221 gctgtcgtct attccccata atttctgcag ttggcgaagg ccattggctt tatgtacgcc
     2281 ggggataatc agatcgatgc tgccgttgcc ggtgtggact ggcaccataa tatcgccgat
     2341 ggcctcatgt aatgcttttt gtacttgtgg aatcagttca tcggaaagat tcaggccaaa
     2401 cttaaagaaa atatcttcta agttgtcaaa gttatcgacg tattccagac ggtgataata
     2461 catttccgcc accgttttca tggcatcgtc atattttttg agtgtatagg cgctattttt
     2521 tccgcaggca ataatttcca cttccgggcg cgtcagcaaa tgttccacga cagtagcaaa
     2581 cgcatccttc gacagctcgc cattaaaaac atctttgcct tcactcatta cccagccgcc
     2641 gttttcagcc acaaaggcaa tttcattcgc aatttcaggg aagaaagaga tcaactgata
     2701 atattgattc ccgctggcga ccacaaagcg aatgccttgt gctttcattt gctgatactg
     2761 agccataaac cgctcacggt tataggtttt ttgatcgctt aagaaagtac catccatgtc
     2821 taccgcaatt aatttaatgc tcatcaacta ttctccatcg ccgtctgcgt tttggtatcg
     2881 ggtttggcga cggctttcgc gacaatagcc gcgagaataa ccagcgccag tacaaccagc
     2941 attgcactac gtaatccata atgttcgccg agatagccca gcagcggcgg cccgacgagg
     3001 aaagccagat aaccggtcgt tgctaccaca ctgacgcggg ttggtgcatc ggggccggta
     3061 tcgctggcgg cagaaatggt cagcgggaag cccagcgagg cacccagtcc ccaaagtaca
     3121 acagacactc ctgcgaccca ggcgctatcg acaaaaataa tcagcccaat acccaacgcc
     3181 cccattagtg cactggcccg aaccacggca acgcgactgt aactgtcgat gaaccaaccg
     3241 ccggtgaagc gtccaacggt catccccagg gtaaaaccgg cataaatcag tgagccggaa
     3301 gtggggctaa aaccgtgacc atcaaccatt aataagggta accagtcgtt ggcagaacct
     3361 tcggcaaagg ccatcgccag cactacaaca ccaatcaaca gcaactggat atcgcgataa
     3421 aaaggaatgc ctttttcgcc atgctgggtg ccatcggcag catttttgcc cgtaccgtca
     3481 gggattgcct gaatggcgat ataaataggc gcgataccta ccagcgccgc caataaaatg
     3541 tgcaccgttg ccggaacgcc aaaggccgtc agtgccatcc cgacaccagc gcctgccagc
     3601 gtgcccaggc tataaaaacc gtgcatcatc ggcaaaaccg ttttattcat ttctcgctca
     3661 acggcggcac cttcaacgtt tatcgccact tccgcagaac caaaacttgc cccaaagacg
     3721 ccgagaccaa cggcaaagag caggggcgat gtcagccaga gtgccagact taatatcatc
     3781 atcccgatca atgcgcagga catcgtgacc aggatgacat tacgtgtccc aaagcgtttc
     3841 actaaccacg ccgagcagag aataccgctc atcgaaccga tcgacagacc aaagagaaca
     3901 ccgcccattt cagcgatcga gacagagaga atatcgcgga tagcaggcgt acgggttgcc
     3961 caggacgcca ttaacaggcc tggcaaaaag aagaacataa acagcgccca ggttcggcgt
     4021 ttcaatgcat tacgtgaaga atttacggtc atagatcacg tcaaaataag aagagggaag
     4081 acaacattag caaggttgtg tacatttgta cacaattgca gacagaggaa atgacatgcg
     4141 tcgcgctaac gatccgcaac ggcgagaaaa aattatccag gccacgctgg aggcggtgaa
     4201 actttacgga atacatgctg ttacgcaccg caaaattgct acccttgccg gggtaccgtt
     4261 gggatcgatg acctactatt tttcaggaat tgatgagttg ttgctggagg cgttcagcag
     4321 ttttactgag attatgtccc ggcaatatca ggcatttttt agcgatgtta gtgatgctca
     4381 aggcgcatgc caggctatca ccgatatgat ctacagctca caggttgcaa cgccggataa
     4441 tatggagctg atgtaccagc tctacgcgct ggctagccga aaaccgctat taaaaacggt
     4501 aatgcaaaac tggatgcagc gcagtcagca aacgctcgaa caatggtttg aacccggaac
     4561 cgcccgcgcg cttgatgcgt ttattgaggg gatgacgctg cattttgtca ccgaccgtaa
     4621 gccgctatcg cgcgaggaga ttttgaggat ggttgagagg gttgcagggt agtagataaa
     4681 ttttagataa caaaaaaccc atcaaccttg aaccgaaatg gcggggttga tgggctccac
     4741 aaaatgggga catcaaagaa aagcagtggc actaattaag actgatgccc tgcggaaaag
     4801 ttctgcggtt gtgcaaaaaa atttcatttt cagggcaact tcagttttat cctaatcctg
     4861 gccataccat gacgatgatt gtccctgcca gcgtcagcag gacgttggcg attgcgtagg
     4921 tgcccgcata gcccagcgca gggatgttac tgcgagctgt atcactgatg atctccattg
     4981 ccggcgcgca ggtgcgtgcg cccatcattg cgccgaacaa cagtgcgcgg ttcattcgca
     5041 atacataagc accgaacaag aaacagataa ccacgggcac aagactgaca attaatccgg
     5101 caatcaacat ctgaccgcca atcgcgccca ggccgttatt aataccgcta ccggcgctca
     5161 gaccaacgcc tgccataaac accatcaagc cgaactcttt caccatgctt aatgcacctt
     5221 gcggaatgta accgaaggtc gggtggttag cacgcataaa gcccagcata attccggcga
     5281 ataacaaccc ggcagcgttc cccatgccga aactgaatgt gctgaactgg aaggtgatca
     5341 tcccgatcat cagcccaata acaaagaagg cgcaaaatgc cagcaggtca gtgacctggc
     5401 tgtgaatcga gataaagccg atgcgatcgg cgatggtttt tacgcgacgg gcatcaccgc
     5461 tgacttgtaa aacgtcacct ttgttaagca cgacgttgtc atctatcggc atctcaatct
     5521 ggctacgaat gacgcggtta aggaagcaac cgtgatcggt caacttcagt tgtgcgagac
     5581 gtttacctac agcgttatgg tttttaacga ccacttcttc agtgacgata cgcatgtcga
     5641 gaaggtcacg atcgaaaact tctttaccgt tacggaagct gggatcgagt cgggcatggg
     5701 cgtcgggata gcctaccaac gctatttcat cgcccatttg tagcacggca tcaccgtctg
     5761 gatttgccag aatcccgtta cgtcgaatac gttcaatgta gcagccggtt tgtcgataaa
     5821 tacccagttc acgcagattt ttgccgtcgg tccaggccac cagctccggg ccgacgcgat
     5881 aggcgcggat caccggtaaa taaaccttac ggttggcatc agtgtccagg ccacgttcgc
     5941 gggcgatttg ctgggcgctg gtctgtaagt cctgatgctg caatttcggc aagtaacgcg
     6001 caccaacaat caaactcacc agaccgatta aataggttaa ggcatacccg aggctcagat
     6061 tatccagtgc cagtgagagc tgcctgcttt ccatgccgga atgatgcagt gtatcgccag
     6121 caccgaccag aaccggtgtc gacgtcatag agcctgctaa cataccggcc gtcaggccaa
     6181 tatcccagcc aaacagctta cctaacccta aggcgatcac cagcgcactg ccaaccatca
     6241 ccagtgctaa cattaggtaa tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc
     6301 cggcttcgac cccgacgcag aaaataaaca gcataaagcc aagattaagc gcatcggtgt
     6361 taatgctgaa atgttgttgg cctaataaca gcgatacgac taaaacgcca atggaattac
     6421 ccagttggat cgaaccaagt cgtaactttc cgagacatag cccaagcgcg aggaccacaa
     6481 ataataacag aatgtaattc ccatttaaca attcggcgac gtttatattc acggaggcta
     6541 acttcttgtt tactagtaag ctgttgaaag aaatgctaat ttacgataat gtgttttacc
     6601 agaattcagg gcgcagattc attcagcgca cctaaacgat agtaaagtaa caatatattt
     6661 tactagtgta atcacattag gtatcaacgg ctatatgaat tgcgttggcc tatattagca
     6721 tggaatgcga agcggcttta tcttactgaa cgccacactg gcgaaaaatg tgttcgatag
     6781 acgcagtgtc aggaggaacg agtgaaacat aaacaacgtt gggcgggggc aatctgctgt
     6841 tttgtcctct tcattgtggt gtgccttttt ctggcgacgc acatgaaagg cgcttttcgg
     6901 gctgccgggc atcctgaaat cggcttgctg tttttcattc ttcctggagc agttgccagt
     6961 ttcttttcac agcgtagaga agtcctgaaa cctctatttg gcgcaatgct ggcggcaccc
     7021 tgttcaatgc tcattatgcg gctgtttttt tcaccgacgc gctcattctg gcaagagctg
     7081 gcatggttac taagcgcggt gttctggtgt gcgctggggg cactgtgttt cttatttatc
     7141 agtagtttgt ttaaaccaca gcacagaaaa aatcagtaaa gccctcaacg cgagggcttg
     7201 tcagacgatc aggcgtccag attttctttc acccatgcag caaaatcggt atagccgccg
     7261 atatgttgct gatcgacaaa aatctgcggc acggtttcta cgggtttacc tgccttttgt
     7321 tgtagatctt ctttagtgat cccttccgca cgaatatcta catactgata ctgaaaatca
     7381 tcgcgttcat tgctcaattt ctcagccaga tcttttgcac gcacacagta agggcaaccc
     7441 gaacgaccaa aaataacggt ttgcattatt tctctcctca tagatttatg cctgtaatga
     7501 tcacgctaaa atgtattcgc tgaaagtagg tttaacctgt tgcattaatt gctaaaagct
     7561 ataactgtta aacacaatac agtgaaaagt tttagactga aggctcactt tgcagaggga
     7621 agcgtatgcg cgcgatcggt aaattgccta aaggcgtgtt gatactggaa tttatcggaa
     7681 tgatgctact ggcggtggcg ctgctgtcgg taagcgactc cctgtcgctg cctgagccat
     7741 tttctcggcc agaagtgcag attctgatga tttttctcgg tgttttgctc atgcttcccg
     7801 ctgcggtggt ggttattctt caggtggcaa aacgtcttgc cccacagctg atgaactgtc
     7861 caccgcaata ttcacgttca gaaagagaaa aagataatga cgccaaccat tgaacttatt
     7921 tgtggccatc gcgccattcg ccatttcact gatgaaccca tttccgaagc gcagtgtgag
     7981 gcgattatta acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg tagtagcatt
     8041 attcgcatta ccgacaaagc attacgtgaa gaactggtga cgctgaccgg cgggcaaaaa
     8101 cacgtagcgc aagcggcgga gttctgggtg ttctgtgccg actttaaccg ccatttacag
     8161 atctgtccgg atgctcagct cggcctggcg gaacaactgt tgctcggtgt cgttgatacg
     8221 gcaatgatgg cgcagaatgc attaaccgca gcggaatcgc tgggattggg cggggtatat
     8281 atcggcggcc tgcgcaataa tattgaagcg gtgacgaaac tgctgaaatt accgcagcat
     8341 gttctgccgc tgtttgggct gtgccttggc tggcctgcgg ataatccgga tctcaagccg
     8401 cgtttaccgg cctccatttt ggtgcatgaa aacagctatc aaccgctgga taaagacgca
     8461 ctggcgcagt atgacgagca actggcggaa tattacctca cccgtggcag caataatcgc
     8521 tgggatacct ggagcgatca tatccgccga acaatcatta aagaaagccg cccatttatt
     8581 ctggattatt tgcacaaaca gggttgggcg acgcgctaaa accgccacgt cgatgtatga
     8641 tacgcgggct tttgaccagg tctgacagag aggtgcaggg tgaaaattgc catattgtcc
     8701 cgggatggaa cgctctattc gtgtaagcgg ctgcgtgaag ccgctataca gcgcggtcac
     8761 ctggttgaaa ttcttgatcc gctttcttgc tacatgaaca taaatcctgc ggcgtcttct
     8821 atttactaca aaggccgcaa gttaccccat tttgacgcag tgatcccgcg tattggcacc
     8881 gccattacct tttatgggac ggcggcactg cgccagttcg agatgctggg gagctatccg
     8941 ctcaatgagt cggtcgccat tgcccgggcg cgtgacaaat tgcgttccat gcaactgctg
     9001 gcgcgtcagg gcatcgacct gcctgtcacg ggcattgcgc attcgccgga tgataccagc
     9061 gatttaatcg acatggtcgg tggtgcgccg ctggtggtca agttggttga aggcacgcag
     9121 ggaattggcg tcgtgctggc ggagactcgt caggcggcgg aaagcgtgat tgacgctttc
     9181 cgcggtctga acgcgcatat tctggtgcag gaatatatca aagaggcgca agggtgcgat
     9241 atccgctgtc tggttgttgg tgatgaagtg gtcgctgcga ttgaacggcg ggcgaaagag
     9301 ggctattttc gttccaattt gcatcgtggc ggcgcggcaa gcgtcgccag tatcacacca
     9361 caggagcgtg aaatcgcgat aaaagccgcg cgaacgatgg cgctggacgt tgctggtgtg
     9421 gatattctgc gtgctaatcg cgggccgttg gtgatggaag tgaatgcgtc gccggggctg
     9481 gaaggaatag aaaaaaccac cggtatcgat atcgcgggta aaatgatccg ctggatcgaa
     9541 cgccacgcta cgacagaata ttgcctgaaa acgggtggtt agtcgcaatc acattactga
     9601 tcatggtttt gcctgcgctt tttgcgtaag ctgtgccggt ctttttatcg aaagaggttg
     9661 tacaaaatta tgacatcgct ggtcgttcct ggtctggata cgctgcgtca atggctcgat
     9721 gacctgggga tgagtttttt tgaatgtgat aactgtcagg ctctgcatct gccccatatg
     9781 cagaatttcg acggtgtctt tgatgccaaa atcgatctga tcgataacac gatcctgttt
     9841 tctgccatgg cggaagtccg accttcagcc gtattgccgc tggcggcgga tttatctgcc
     9901 atcaatgcca gttcgctgac cgtgaaagca tttcttgata tgcaggatga taatctgcca
     9961 aagctggtgg tttgccagtc tttatccgtt atgcagggcg taacctatga gcagtttgca
    10021 tggttcgtgc gtcagagcga agagcagatt tcgatggtca ttcttgaagc taatgcccat
    10081 caactgctgt taccgactga cgatgaaggg caaaacaacg ttaccgaaaa ctattttctc
    10141 cactgataat tcctttcgag cacgcagtcg ctgccgcagt gactgcgcgc tgcaaaatta
    10201 tctgctgttt ttaacctttt cttaaagatt atttcacttc tcttgtgtcg atttggcttt
    10261 atcacataga gcaaatacgc ataaaaattt gttaaatacc gttttttaat ccgagctata
    10321 gtctcaaacc ctggctaaag ttattcttgc gatgcttcta tatagcgagc agtgctggcc
    10381 gggagagagt tctcttttct tacaccgcgc cgataaaaaa atatgcacgt ttattgcata
    10441 tctttcagtg tgacaacttt tgttcgtttg ttaacgaact ttcagaagga aagagatgtg
    10501 accgccttaa ataaaaaatg gctatcgggt ctggttgcgg gtgctctgat ggccgtctct
    10561 gtcggcacgc tcgcggctga acaaaaaaca ctccacattt ataactggtc tgattatatc
    10621 gcccctgaca cggtggccaa ttttgaaaaa gaaaccggta ttaaagtcgt ctacgatgtt
    10681 ttcgactcta acgaagtact ggaaggcaaa ttaatggcgg gtagcaccgg ctttgacttg
    10741 gtggttccat ctgccagctt tctagaacgc cagttgactg cgggagtttt ccagccgctg
    10801 gacaaaagca aattgccgga gtggaagaat ctcgacccgg agctgctgaa gctggtcgcc
    10861 aaacacgatc ccgacaataa atttgctatg ccctatatgt gggcgacgac cgggattggc
    10921 tataacgttg ataaagttaa agctgtgctg ggcgaaaacg cgcccgtcga tagctgggac
    10981 ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc tttcctggat
    11041 gcgccagaag aagtttttgc taccgtgttg aattatctcg gcaaagatcc caacagcact
    11101 aaagcggatg attacaccgg accggcaacc gatctgctgt taaagctgcg cccgaacatt
    11161 cgttatttcc attcatctca atacattaac gacctggcaa acggcgatat ttgcgtcgct
    11221 atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg cgaaggaagc gaagaatgga
    11281 gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg cgttctttga tgtattcgcc
    11341 atgccagcgg atgccaaaaa caaagacgaa gcctatcagt tcctgaatta cctgctgcgc
    11401 ccggatgtag tagcgcatat ttccgaccat gtgttctatg tcaacgccaa taaagcagcc
    11461 acgccgctgg tgagtgcgga agtccgtgag aacccgggta tttatccgcc tgcggatgtt
    11521 cgtgcgaagc tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc
    11581 gcgtggacca aagtgaagag cggaaaataa tccgcagcct tagatgccgg gcgggcgcac
    11641 cacacccgcc ggcaattcgc accatcatgg tgcgcttgca cacattcaat gccggagagc
    11701 agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggcgc tgacgccgct
    11761 attagaaatc cgcaacctca ccaaatccta cgatggtcaa catgcggtgg atgatgtcag
    11821 cctgaccatc tacaaaggtg aaatcttcgc gctgctgggc gcatccggct gtggcaagtc
    11881 cacgctgctg cgtatgctgg caggtttcga acaaccttct gccggacaga taatgcttga
    11941 tggcgtcgat ttgtcacagg ttccgcctta cctgcgcccc atcaatatga tgtttcagtc
    12001 ttacgcgctg tttccgcata tgaccgtgga acagaacatc gcttttggcc tgaaacagga
    12061 caaactaccg aaagcggaaa tttccagtcg ggtcaatgag atgctcggcc tggtgcacat
    12121 gcaggagttc gccaaacgta aaccgcatca gctttctggt ggtcagcgac agcgtgtggc
    12181 cctggcccga agccttgcca agcgcccgaa actattactg ctcgacgagc cgatgggcgc
    12241 gctggataaa aagctgcgtg accggatgca gcttgaagtg gtggatattc tggagcgcgt
    12301 cggtgtgact tgtgtgatgg tcacccacga tcaggaagag gcgatgacca tggcggggcg
    12361 catcgccatt atgaatcgtg ggaaatttgt ccagattggc gaaccggaag agatctacga
    12421 gcatccgact acccgctata gcgccgaatt tattggctcg gtaaacgtct ttgaaggcgt
    12481 actcaaagag cgtcaggaag atggcttggt gcttgattcg ccggggctgg tgcatccact
    12541 gaaagtcgac gcggatgcct cggtggtcga taacgtgccg gtacatgtgg cgctgcgccc
    12601 ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt tgtaacttcg cggtggggga
    12661 ggtgatacac attgcctatc tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg
    12721 gcagatgatt agtgcccagc tacaaaacgc ccatcgtcat cgtaaagggt taccgacctg
    12781 gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt gtggtgctga cggtttaagg
    12841 agcaaagatg aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg gatttaagct
    12901 gtggttgtcg cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt tgccatatat
    12961 ctggttgatc ttgctgtttc tgctgccatt tctgattgtc tttaaaataa gcctggcgga
    13021 gatggcgcgc gctattccac cttataccga actgatggag tgggctgacg ggcaactgtc
    13081 catcactctt aatctcggta attttctgca actgaccgac gatccgctct atttcgatgc
    13141 ttatctccag tcgctacagg tggctgcgat ttcgacattt tgctgtttac tgatcggcta
    13201 tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc cgtaatattt tattactact
    13261 ggtgatcctg ccgtcgtgga cctcgtttct gatccgcgtt tatgcttgga tgggaatatt
    13321 aaaaaacaac ggtgtgctga ataattttct gctgtggctg ggggttatcg atcaaccgct
    13381 gaccattctt cataccaatc tggcagttta tattggcatt gtttacgcct acgtgccgtt
    13441 tatggtgctg ccgatttata ccgcgttgat tcgtattgat tattcgctgg tggaagcagc
    13501 gctggatctc ggtgcacgac cgctgaaaac gttctttact gtgatcgtgc cgctgactaa
    13561 aggtgggatt attgccggat cgatgctggt gtttatcccg gctgtgggcg agtttgtgat
    13621 cccggaactg ctcggtggcc cggacagcat catgatcggg cgcgtgctat ggcaagagtt
    13681 tttcaataac cgcgactggc cggtggcctc ggcggtagcg atcatcatgt tgctgctgct
    13741 aattgtgccg ataatgtggt tccacaaaca ccagcaaaaa agcgtgggag aacacggatg
    13801 aataatttac cggtagttcg ttcgccctgg cggattgtga ttttgctgct gggctttacc
    13861 tttctctacg cgccaatgct gatgctggtt atctattcgt ttaacagctc gaagctggtg
    13921 acggtgtggg gcggctggtc aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg
    13981 atgagtgcgg ttggttcaag cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc
    14041 ctcgggacta ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc
    14101 tttgccttta tgatcaccgc gccgctagta atgccagatg tcatcacggg cttgtcgctg
    14161 ttgttattat tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg cggtatgctt
    14221 accatctggc tggcgcatgt cactttctgt acggcttatg tggcggtcgt tatttcgtcg
    14281 cgtctgcggg aactggatcg ctcgatagaa gaagccgcga tggatctcgg tgcgacgccg
    14341 ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat ttctggctgg
    14401 ttactggctt ttactttgtc gcttgatgat ctggtgatcg ccagctttgt ttccgggccg
    14461 ggagccacca cgttaccgat gctggtcttt tccagcgtgc ggatgggggt gaatccggaa
    14521 atcaacgccc tggcaacatt gatcctcggc gcggtcggaa ttgtcggatt tatcgcctgg
    14581 tatctgatgg cgcgcgcaga aaaacagcgg atacgcgaca tccaacgtgc aagacgtggc
    14641 tgaagacact aaaatttgcc aacctggcta cataatgccg cgcatgtcgc ggcattgttt
    14701 tcatggaaga cgaaacgttg ggatttttta agaaaacatc ttcatctcat gctcgcctga
    14761 atgtgcctgc gctggtgcag gtggcggcgc tcgccattat tatgatccgt ggcctcgacg
    14821 tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg
    14881 tacaaacctg gagtttaacg ctggtctttt taagcagtct ggtgctggtt ttcattgaga
    14941 tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc gcgctggcta tatctgctga
    15001 cacaaatcac cgccgcaagt tacttgtggg cggcttcgct ggggtacggt tatccggagc
    15061 tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc
    15121 tgccggatat gctcatcctg atgctgctgt tcgttccctc gaccagtcgg cggttcttcc
    15181 agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc
    15241 gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc gattccagag
    15301 caactctccg ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa
    15361 tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta ataaagccaa aatggtggtg
    15421 agtggtagcg ttgaaaaaac actgctcggt atgctgcatc gcgatggcac gccggaagac
    15481 ctttgtgact gcccgcttta tccagcctca tttgcgcccg tttttgcggc gctaaaaccg
    15541 tttatcgccc gagcggggtt aacgccctac aacgtggcgc gtaagcgtgg cgaactgaaa
    15601 tacattctgc tgactgaaag ccagagtgat ggcggcatga tgctgcgctt tgtactgcgt
    15661 tctgatacca agctggcgca actgcgtaag gcgctgccgt ggttacacga acaactaccg
    15721 cagctgaaag ttattaccgt caatattcag ccggtacata tggcgattat ggaaggggag
    15781 acggagatct acctgaccga acaacaggca ctggcggagc gttttaatga cgtaccgctg
    15841 tggatccgtc cgcaaagttt cttccagact aatccggcgg tcgccagcca gttgtacgcc
    15901 accgcgcgcg actgggtacg acagctgccg gttaaacata tgtgggatct gttctgcggt
    15961 gtggggggct ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt
    16021 gcgccagagg ccattgcctg tgcaaagcag tcagccgctg aactgggctt aactcgttta
    16081 caatttcagg cgctggactc cactcagttt gccactgctc agggggaggt gccggagctg
    16141 gtgctggtta acccgccgcg ccgcggcatt ggtaaaccgc tgtgtgatta tctttcaacg
    16201 atggcaccgc gttttattat ctactccagc tgtaacgccc aaaccatggc gaaagatatc
    16261 cgcgaactgc caggttaccg catcgaacgg gtacagcttt ttgatatgtt cccgcacacc
    16321 gcgcactatg aagtgttgac gctgctggtg aagcaataaa aaagccacag gtgcggcttc
    16381 agattgctga tcaggtgcgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    16441 tacaaaatca tgctaaatca atacattgca gagttgatgt gggccggata agcgcagcgc
    16501 atcaggcaat cttacttttg tcatcagtct caattactgc ggaaaccact ggtcattgat
    16561 tttctgatat gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag
    16621 agctttgtta tccggacgta cagcgatacc caggccggtg ccgaagtatt gcggatcggt
    16681 cactttttca gtcgcaacgc ccagttgcgg attggttttc agccattcgt ttaccaccgc
    16741 ggtgtcacca aataccccat caatacgacc atttttcaga tcgataaagg cattctgata
    16801 actgtcatag gagacagttt tcacttccgg gtgctgatcc tgaatatatt tctggtgcgt
    16861 cgtaccgttt tccatcccaa tgcgtttgcc tttcaggtcg gcaaacgttt tgtaggtatc
    16921 ttttttggaa atcacgacgg ctgagttttc atagtagggt gtggtaaacg aaacctgctt
    16981 gctacgctcc ggggtgatat ccataccgga gattacggcg tcatattttc tgaatttcag
    17041 tgacgggatc aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt
    17101 gcacaaggct tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga
    17161 ttcaaagggt ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc
    17221 gaaagtaaag gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc
    17281 tgtcaatctt atgatttttg gccgtgtctg cggcatggga taacaatgcc atcaagtgaa
    17341 tttatatgca ataaacatga ttaaataatt taaatgaaat aaaaaaagac ggataactta
    17401 gtgagttgtc cgtcttcatt ataagaattt atgcactatg taggccggat aaggcgtcca
    17461 cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag
    17521 acctacaaac ccccccggcg aatgtacgca gctacattaa ttccgccgtt cgaatgccag
    17581 tgctttgcgc tcgatcagac gcatcatcag cgtcagcagg ccgttgacga ccaagtaaat
    17641 aatccctgcc gcaccgaaca ccattacatc gtaggtgcgt ccgtacaaca actggctgta
    17701 tcccatcact tccatcagcg taatggtgta agccagagag gtacttttga acaccagcac
    17761 cacttcgttg gaataagaag agagcgagcg tttaaaggca tacggcagca ggatcgccag
    17821 cgtatctttt ttgctcattc ccagggcgct acaggactgc cactgacctt ctgggatcgc
    17881 acgaattgca ccgtaaaaca gctgcgtggt atacgccgca ctgttcagcg ataacgcaat
    17941 cagcgcacat aaccacggtt ctgacaacaa atgccacagt gccggatact cctgcaacgt
    18001 cggaaactgg cccggcccgt aataaatcag gaagatctgc accagcagcg gcgtaccggt
    18061 aaacagcgtg atataacccc gcaccagcca caccagcacc ggcgttttca gcgtcaggat
    18121 gatggtaaaa atcaatgcca gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag
    18181 gctggtgtgc agccctttca tcagctcggg taaatactca aacattagct gggcctccgc
    18241 tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt actgactgag cagggtgatc
    18301 accaggtaaa tcgccgccgc cacaatgtac caggtaaatg gttcctgggt acgagtagca
    18361 atgcttttgg tttgtaacat taaatcattc acactaatca aactgaccag cgcggtatct
    18421 ttcagcagca ccagccactg gttaccgagg ccaggcagcg catgacgcca catctgcggc
    18481 atcaccagac ggaaaaagat agccgatttc gacagcccca gcgcctgacc agattcccac
    18541 tggcccaccg gtacagcttt caacgcgccg cgcagcgttt gcgaggcata ggcggcatac
    18601 agcagtgaca gagcgatgac accacaaagg aacggactca cgtcgaagtt ctcaatgtcc
    18661 atctgcaccg ggatctgcac gaacccaaga ttgatagtga agccatccga aagcgtcagc
    18721 agtaactgcg aggagccaaa atagataaac agcactacca gaatttctgg caggccacgc
    18781 agaatggtta ccagcgctga acctgcccac gcgacaggac gccatttcgc tgactcccat
    18841 accgcaaaga acatcgccag cgccagcccg acaatcaatg cacaaacggc aaggccgacg
    18901 gtcatcccgg cggcgcttgc taaaggaaaa aattcattca tcaggaatta cttctggaac
    18961 catttgttgt agatggtttc gtaagtgcca tctttcttca ctttttccag cgcagtgttg
    19021 agtttctgct gcagttcagt gtttccctga cgtaccgcga tgccgaggcc agtaccgaaa
    19081 taatctttat cggtcacttt atcgcccact gccgccagtt ttgggttatc tttcagccac
    19141 tcagtgacca ctgcggtgtc accaaatacg ctgtcgatac gcccgttttg cagatccagt
    19201 tttgcgttct ggtagctgtc atacggaacg gtgatgattt ccgggtgctt atccataatg
    19261 aacttctggt gtgtcgtccc gttctgaacg ccgacttttt tgcctttcag ctgatcaaca
    19321 ctggtgtatt tgccttgctg acccacaaac agggcagagt tgtcatagta cggggtagta
    19381 aacagtacct gcttttcacg ctccggagtg atatccatgc ccgccatcac tgcttctacg
    19441 cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct ggttagagaa agtacaggtt
    19501 gcatcaatct ctttacacag tgcctgcgcc aggtcgacgt caaaaccaac gatctggttg
    19561 tttgcatcaa tcgattcaaa cggaggatag ggggcttcgg tagcaaaacg aatggtttcg
    19621 gcagctgtgg cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt
    19681 gttgttatcc cgaatcttag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg
    19741 taaagcagct cgcgtcgcct tgttctacga tatgaccatt ttccatatac accactcggc
    19801 tggcggtttt acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt
    19861 ctgccagctc acgaatgatg ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg
    19921 tcggttcatc gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac
    19981 gctgctgctg accaccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca
    20041 gacgttccag cagtttttct gcacgggcca gcgcctgatc tttactcaac cccagtacac
    20101 ggcagggcgc ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct
    20161 gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg
    20221 tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat
    20281 tgagtacacg cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt
    20341 cgccctgtgg gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat
    20401 taatgccgtt taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga
    20461 ggtcgaagat agtaccttta acagaataat tatgcaatat ttatgcttta aaagttaaaa
    20521 gcaaagcgca ttattcaata aacatagcac aaaataacgg gggcggtggt cggtgagcat
    20581 aaatgtcggc attcctcacg aaatgccgga taatttacgg ggtttattgg ttgatcaagg
    20641 cgttagcgat tctcgatgga ctgacggagc gtacccgccg tggcatgaac gctaccgcct
    20701 aagtagcgca catcgtcgat gacccaacac tggccttcct gaatcattaa cacttcatcc
    20761 tgccagccct ggtcaccctg tttgagatcc acccgcaatg gaatgttacg ggcatcacga
    20821 ttagggatag tcgatgcact ggcaacgtgg gcgctgtctg gcaaggtggt tcgactggag
    20881 aacggatcgc tgcttaacaa ttcacgatga ctattatcgc ggctggcatc gctaagttgg
    20941 gttgccagtt tgtcactcaa ataggggcgc agggcggtaa tgtcattgct gcgatgttga
    21001 atgcggtagt cgtagaattg ttgagccaca ctgtcaggcc ctccctggac acaggaaccg
    21061 gtgcgggggc cgttatcttt ataagctggc gtgacggtgg tacaggcact cagcagcagt
    21121 gcacatggaa taagtagaga tagtgaacgg tagcgcataa tcatttcctt attagcttgc
    21181 tgtctgcgtc acactacgac caaacgctat cagattattt aatacattgt ttttaataca
    21241 attctgaaac aatctgaaca cctgattggg tttgagtcca ttgctggcga cccactcaag
    21301 gttgctcgta gctgacttac ttaaattacc caaacaatag acagtgtaaa ccatttgtga
    21361 ggttgagcgt gttatatggc tgaaatgatc atttaaggta aatatgatag ttctgaaatg
    21421 gtggtataaa tcctcctgta gaggttaaga gcatctgcaa gaagccagtt tcacatttat
    21481 aacgcgtgat aaatgaatgc ggttattgtc taatcattac aaaatttttc acatgcgata
    21541 agagatcctg cgattaagat gattgccaaa attacgttgt atttttgaaa atgagcgaaa
    21601 tttgatagtc ccagctttga ctctcgataa tattatttat gctacaattg tgggtaaaga
    21661 atttaagcta atgaatttga gtgaagaata aattaaattg cttcagtatt tcgttaatta
    21721 atataaatac aaaaggcgtt gtgcttttat aattataaag cgcgaggagt tcggtggatt
    21781 atcgtttttc ttgggtgatt tttaatatga taaacaatat atctgatcaa gcatcatcat
    21841 ttccaggcac acaactaaat caaagtgata atttcctgga ttcactgagg gaatttttcg
    21901 cgatattaaa tcccagtcgt aagggtgagt tgtctacatg ggataccatc tacttgcatc
    21961 tgatattagc aataaatgct gacagtgatc tcataaaaaa tgatgtgttg ctcgcggaaa
    22021 atattccatc agcgaattac caatttaata cattttttag caatactttc gagattgatg
    22081 ttaaaaaata tctgggtaaa tcagaagata acgaagttga aataaaagcg ggtaatgaaa
    22141 gaatctctat tggaatacgt aatgtatcga atggaaaact tgaaagacaa cagtttcttt
    22201 tcccactgga ctatgagaat aaattacaag aacaattaga taagtatttt acgattgaat
    22261 cgcacccatt attatacagg tataccattg gtagtaagat tgcgaatgtg atttttgaaa
    22321 agttatacag tcgtattgac tttaacaaag aacaatatat atcctttata aaggacgcat
    22381 ttattcattt ttatgattat agcaggcgat atgctattag tgagaatatc gataaagacg
    22441 ctgtaacaaa caatatcgca ttgatgagca ctttttatga cagtgataat acgtcaggtg
    22501 aggttttaaa taatgatttt acagaagaag agtcatttga aactgcgtta gatgtagagc
    22561 atgcaatagt gcttggattt gctgatgata attttgaaac aaaacctgtt cattatcagg
    22621 atttattaac acgatttagt gcctttcaag ataccgtgtt taatctgttt cctgaaatgc
    22681 actcttcgca ttaccacgat atttgtagcg taagtgtgga tatgacaaag ggaacgcaat
    22741 gcatgattca cttgatggtg aatgaagaag ttttcatgtc tctgcctgtt ccagttgcca
    22801 cgatggtacg tgaagatgca agtaatttag tcaatcttaa aacactactc aatgatggtt
    22861 gtttcattaa atatagtcat tttaatgacg tggcattaat aaagcaaaac ataagtaatc
    22921 tctatttatc acacactgtt gtgaatgaaa gcatattgaa aaaatgttgt tttgaaaacg
    22981 gcagtttagg tgatgttaaa ataacaaata gcaatgtaat aaacagtgca ttcaaaaata
    23041 tttcgtttag atctgttaaa attaataacg taaatactca ttctttaaaa tttattaact
    23101 gcaatttttt caatgttgat atgataagag ttaatctcag caaatgtctt tttcatgaat
    23161 gtagtatgca tggtgtcaag ataaaaccct ggttgcctgt aaaatggaca aaagaattaa
    23221 taagtgatta tttgtatggc tgtttactat ctctgtactc tatctgtgca agggatatat
    23281 ataatatgaa cgccgggaat aacgtgaagg ttgctgcgga tgctttcctt gaaattattt
    23341 tctctcttaa aaataaatat tgtattaaac tattaagcgc tcaggatcgg gcgtttatat
    23401 acgagtttgc aagaatgatc ttcgcttata ttaatgacaa aagcattgag atcttattac
    23461 tttcttgttt tgcggcggct gaccaaaaag caatccagcg gtatagacct cagtcacaag
    23521 atggggaaga ctttaggagt catttacaat ataagttgcc tctttccgct cattaatgtt
    23581 gttggtgcaa cataataaga caggtaagcg gtaccctata atgaaaccaa tatcgggtta
    23641 tgcaacggat aatcgtaaag acttaagtgg gtgtttttga cacaatgaga tgatttccta
    23701 aactgatcaa aaccttcttc ccaacatggc atatcagtcc gataatgctt tagagatcga
    23761 aggcttaaca aacaaggaga tcgatcatgc aattttctac aaccccaact ctggaaggcc
    23821 tgaccatcgt tgaatattgc ggtgtggtga ccggcgaagc gattttaggt gccaatattt
    23881 tccgtgattt ctttgccggt atccgcgata tcgttggcgg acgttccggt gcgtatgaaa
    23941 aagaactgcg taaagcacgg gagatcgcct ttgaggaatt aggctcccag gcgcgggcgc
    24001 tgggggccga tgccgtagtt ggtattgata tcgactacga aacggtcggg caaaacggca
    24061 gtatgctgat ggttagcgtc agcggtacgg cggtgaaaac gcgtcgatga gaagagtctt
    24121 ctggctggtc gctgccgctc tgttactggc agggtgtgca ggcgaaaaag gcattgtcga
    24181 gaaagaggga tatcagcttg atacccgaca ccaggcgcag gcggcgtatc cgcgcattaa
    24241 agtgctggtg atccactaca ccgcagatga ttttgatagc tcgctggcga cactgactga
    24301 taagcaggtc agctcgcatt atctgatccc tgcggtgcca ccgcgataca acggtaaacc
    24361 gcgcatctgg caactggtgc cggaacaaga actggcctgg catgcgggga ttagcgcctg
    24421 gcgcggggca acgcgcctta acgacacctc tattggcatt gagctggaaa accgtggctg
    24481 gcaaaaatca tccggagtga aatattttgc cccgtttgaa caggcacaga ttcaggcgct
    24541 tattccgctg gcgaaagata ttattgcccg ttatcacatc aagccggaaa acgtagtggc
    24601 acatgcggat atcgcaccgc agcgcaaaga cgatccgggg ccattatttc cctggcagca
    24661 actggcgcag caggggattg gtgcctggcc ggatgcgcag cgggtgaact tttaccttgc
    24721 cgggcgcgcg ccgcacacac cggtagatac cgcgtcattg ctggagcttt tggcgcgcta
    24781 cggttatgac gtaaaacctg atatgacacc gcgcgagcag cggcgcgtga ttatggcatt
    24841 ccagatgcat ttccgcccga cgttatataa cggcgaagcg gatgcagaaa ctcaggcgat
    24901 tgccgaagca ttgctggaga aatacgggca ggattagcgc ggcagttttc cgtggtcgcg
    24961 tagccaggcg gcagttttct cgatgccttc atccagggtg atgaccggct gataacctaa
    25021 ctcttcctgc gcacgcgtaa tatccagcgt aaagtcaaaa ttcaacttgg aggcgccgta
    25081 gtgggtcagc ggcggctctt ttgctgactt gcggcctaaa cgctccatgc tgcgggcgat
    25141 catatccagc atcgggtaag ggacggaacg aatacgacag tcaatattca actcgtcgat
    25201 cagcttctgc acgatgctgc gcagtgtgcg atgctcgccg ttggtgatgt tgtacacacg
    25261 cccggaaggt agcttatcgc aggcttcctg gcttgccagc cacattgcgt gcacggcatt
    25321 ttcatagtag gtcatatcga ccagcgcact gccgccatgc ggtaacagaa tactgccgta
    25381 gtggtgcatc atatgcgcca gacggggaat aaagacttta tcgtgcggtc cgaacagact
    25441 ttgtgggcgc agaatagtaa agcgcgtttg tggattcgcc tgcgaaagca tattgatcac
    25501 ttcttcgctg gctgctttgc tgcgggcaaa ctcgttggcg aagcggtgag ggcgaaaatc
    25561 ttctttaata ttgcgatggt ggtgataatc gaagtacagg gagggggaag agatatgaat
    25621 aaagttacgc acaccccagg cgacagccca ttcacccagg cggcgagtgg cgcgaacgtt
    25681 agccagatcg aaagcctgtt gtgtccccca gggtgaggta aagctggagc agtgccacag
    25741 cgtatcaatg cccgcgagca tcactttagc ttgtgatgaa accagctcgg tcagatccgt
    25801 cggaacaaac tctgcgccca ttttttccag caatttaccc attgcttcgt tgcgaccgct
    25861 cgcacgcacg ctgatgcctt tctggcataa aaactctacc gcgtttcgac ctaagccgct
    25921 ggtggcgccg gtaaccagta ccttcatatc aatccactgt tgttgagaaa atgacgtgcg
    25981 cattcttccg tgatttcccc catgatgcaa tgggaaacat gaaagaataa cgcaggtttt
    26041 gtcgattaat ctgtgctttg ttctgccagt ctggcgattt gttttgccat tccgcgaaaa
    26101 ataaacagat gcgcggggat catcaataac cagtaaaaca gccccggcat accgtgcgga
    26161 tgccagaaag cgcggacatc gatagtacga tagtcacctt tatcttccag ggtaaaacac
    26221 agtcgtccca gccccggcgc tttcatgcca aataacaacg taagttgctt tttcggttca
    26281 acgacaatca ctttccagct atccaccgca tcgccggtct gtaaatattc gcgctccggg
    26341 cggcctttcg ccagattatg accgatcgcg cggtccatca acgcccgtgt ctgccacaaa
    26401 atattgccaa agaaataacg ctctttaccg ccgatttggt tcaccaccag ccataaagcc
    26461 gcaaggctgg cggacgtttt aacggtaaac cccgcctgtt tggcgaaata accgtactcc
    26521 ggtcgccagc gggcaaaggc ctgagcgtcg tagccccagt cgctggagtt gaccagtttt
    26581 tcttcctctt tcaacgtgct acgtaccgcg tcatcgaaag cgatcagccg ttgtgggatg
    26641 agtgcacgta gcgcggtatc atctgccagc agatcgtgtt tcagcccctg aatcaacgcc
    26701 ctggcggtgg tgggcggtac ggaagtaatc acattgagaa accacaccga aatccagcgg
    26761 gtggggaggg ggatggggat caaccagcgg cgcttaccgc tcaccgccat aaaatgttca
    26821 aactgttgct gataactgag cacctctggt ccggcggctt cgaagatgcg gtgttcgttg
    26881 gctggatggt ctaacagcgc caccagatag tgcagcaagt tttccagcgc gatgggcgtg
    26941 gtgcgtgaac gtacccagcg tggcggcgtt aacactggca ggttgtagac catatcgcgc
    27001 atgacttcga acgccgctga acctgcgcca acgataattc cggcccgaag ttcggtcact
    27061 ggtacattcg cttcacgaag aatgtccgcc gtagcctgac gagctcgcag atgatccgac
    27121 tgctcatgtg gcggggcctg caacgaactg agaaagatta attgcttaac tggtacttca
    27181 cgtagcgcat cgcggacgtt gagagccagc tggcgctcct gagcgataaa atcgccgcct
    27241 tcgcccatgc tgtgcaccag aaaatagacg gtatcgatat cctgcaacag ggccggaagg
    27301 ttatccggcc agctgagatc gactttatgg caactgacgt ttgccagttg cagctttgca
    27361 agcctgtcga catgacgtgc cgccgctagg atctgatgcc cttgctggct gagtgtgcgc
    27421 accagatgct gaccaatgta gccactggca ccgagaacta aaatgcgttg cggcacgtct
    27481 ctctccttaa cgcgccagga atgcgcgcca gtgggcggcg acttccgcca gttgttcgcg
    27541 cgagacgtca agatgcgtca ccaggcggac aatcggcgag gcgttaatca gcacgtttct
    27601 cgctttcatg tattcgccta acgcggcagc attttcttcc ccgacgcgaa caaacagcat
    27661 attagtgtcc tgacgcatca catccgcgcc tgcttcacgc agttgctccg ccatccaggc
    27721 ggcgttgtcg tgatcttcct gcaaccgtgc gacgttattt tccagggcat acatcccggc
    27781 ggcagccaga atcccggact gacgcatccc gccaccggcc attttccgcc agcggatggc
    27841 acgtttaatg taatcacgat taccgacgag taatgaaccg actggcgtcc caagaccttt
    27901 cgacaggcaa atggtgaacg aatcacaata ttgcgtgatc tctttcagtt cgcagccgta
    27961 agccaccacg gcattaaaga tgcgcgcacc gtcaacatgc agcgccagat tgcgctcgcg
    28021 ggtaaattcc catgcttctt tcaggtattc acgcggcagc actttgccgt tgtgggtgtt
    28081 ttccagactg agtaatttgg tgcgggcgaa atggatatcg tcgggtttga ttttcatcgc
    28141 cactttatcc agcggtagcg tgccgtcggc agccgcgtcg atgggttgcg gctgaatact
    28201 gcccagcacc gctgcgccac cggcttcaaa cagatagtta tgcgcggcct gaccgacaat
    28261 atactcttcg ccacgttcgc agtgactgag cagagcgacc aggttggcct gagtgccggt
    28321 aggcagaaaa atggcggctt ctttaccgga aagctctgct gcatagtcct gcagagcatt
    28381 aacggtaggg tcgtctccgt aaacgtcgtc cccaaccggg gcggccatca tcgcttcgag
    28441 catggcgcgg ctcggtcggg taacggtatc actgcgtaaa tcaatcatgg catgtcctta
    28501 ttatgacggg aaatgccacc ctttttacct tagccagttc gttttcgcca gttcgatcac
    28561 ttcatcgccg cgtccgctga taattgcgcg caacatatac aggctaaaac ctttggcctg
    28621 ttcgagtttg atctgcggtg gaatggctaa ttcttctttg gcgaccacca catccaccaa
    28681 caccggaccg tcgatagaga aggcgcgttg cagggcttca tcaacttcag aggctttttc
    28741 tacacggata cccgtaatgc cgcacgcttc ggcaatgcgg gcaaagttgg tgtcgtgcag
    28801 ctcagtaccg tctgtcaggt aacctccggc cttcatctcc atcgccacaa agcccagcac
    28861 gctgttatta aagacgataa ttttcaccgg cagtttcatc tgcactactg agaggaaatc
    28921 gcccatcaac atgctaaaac cgccatcgcc gcacatggcg accacctgac gttccggctc
    28981 ggttgcctgc gcacccaacg cctgcggcat ggcgttagcc atcgaaccgt ggttaaacga
    29041 acctaacagg cgacgcttgc cgttcatttt cagataacgt gccgcccata ccgttggcgt
    29101 gccgacgtca caggtaaaga tggcgtcatc ggcggcaaaa tgactaattt gctgcgccag
    29161 atattgcggg tgaatggctt tctcgctcgg tttagctaaa tcgtccagcc ctttgcgggc
    29221 atcgcggtaa tcttccagcg ctttatccag aaacttgcga tcggtttttt cttccaccag
    29281 tggcagcaat gcgcgcaggg ttgatttgat atcgccgacc agcgccatat ctaccttgct
    29341 atgcgcgccg atgctggctg gattaatatc aatctgaata attttggcat cggtcgggta
    29401 gaacgcacgg taggggaact gagtaccgag tagcactaac gtgtcggcgt tcatcatggt
    29461 atggaaacct gacgagaatc cgattaaccc ggtcattcca acatcatacg gattatcgta
    29521 ttcgacatgc tctttaccgc gtagggcatg aacaataggt gctttaattt tcccggcaaa
    29581 ctcaactaac tctttatgcg cccccgcgca gccgctgcca cacatcaggg cgatattgct
    29641 ggaataacgc agcagttgcg ccagtttgcg taactcttct tcttccggca tcacgactgg
    29701 ctgtggcgca tgataccagt gcgtggttgc cccttctggc gcaggtttta acgccacgtc
    29761 gcccggtaat acaacaacgg aaacgccacg gttaagcacc gctttgcgca tagcaattgc
    29821 cagcacttgt gggatctgct ccgggctgga aaccagctcg caatagtgac tacattcgcg
    29881 gaatagctct tgtgggtggg tttcctggaa atagccgctg ccaatttcgc tggagggaat
    29941 atgagcggca atcgccagta ccggaacgtt attgcggtgg caatcgaata ggccgttgat
    30001 caagtgcagg ttgcctgggc cgcacgatcc ggcacagacc gccagttctc ctgaaagttg
    30061 tgcttcagcg ccagcggcaa acgccgccac ttcttcatgg cgggtggaca tccactcgat
    30121 ggtgcccatg cgattaagac tgtcgctaag accgttcaga gagtcgcccg tgactcccca
    30181 gatgcgtttc acgcctgccg attcgagtgt tttggcgata taagctgcaa ccgtttgttt
    30241 catggttctc catctcctga atgtgataac ggtaacaagt ttagttcatc tgacggaggg
    30301 ggaagggatg ggagagaaag gaggcactaa cggttaaata gcccgatgaa aggaatatca
    30361 tcgggcataa ggcgattatg cgagaaccaa atccccctgc ggatggcagg agcaggccag
    30421 tacgtaacct tcagcgattt cggcgtcggt cagcgtcatt gtactgctca ccgtatattc
    30481 accggaaacc acttttgtct tacagcagcc gcaaacaccc gcacggcagg cagcgacaac
    30541 cggaacgtta ttgctttcca gcgcctccag tagcgtggtg ccaaccgggg cgtaaaattc
    30601 tcgtgccggt tgcagtttgg tgaatttcag accgctggtc gccgcttctg ctactggggt
    30661 gaagaatttc tctttaaaga aacgcgtcac gccgagcgct ttcacttcct gctctaccca
    30721 atccatatac ggagccgggc cgcaggtcat cacggtacgt gaagctaagt caggtacatc
    30781 tgccagcagt tcgcgagtga ggcgaccagc gataaagcct tcggtaacgt tattttctgc
    30841 caccagcgtt accggatagt tacgccactc atcggcgaaa ataacatcct gcggcgtacg
    30901 cacgttgtag atcacccgca catcggcctg tggacggttc ttcgcaagcc agcgacgcat
    30961 cgacataatc ggcgtgacgc cgcagcctgc cgccagcaac aggaatttat cttctgcttt
    31021 atcgtcgcag gtaaattccc ccatcgcgtc cgaaagccag agataatcac cgcgttttac
    31081 atcgcgcgtc agccactggg agccgacacc gtcatcaatc cgccgcacgg tcagggtgat
    31141 atattcactc acgcctggcg tggaggaaat ggtgtaagca cgcagcgttt ccgctgagtt
    31201 tcgcacgctg accagtgcat attgcccggc gcgatatggg tagtgatcgt ggcaaatcag
    31261 ggaaatcgtc cacacatccg gcgtttcttg cgtaatgtga tgaacctgta tccgccacgg
    31321 gcattgattc gttggcatcg tcatcgacaa actccttacg cgttcaacag ttgcttcatg
    31381 tcttcttcaa cagtggtgat agaacgcagg ccgaatttct cgttcagcac cgccagcagg
    31441 tctggtgtca ggaaaccagg tgcagtcggg ccggtgacga tatttttcac gcccagagaa
    31501 agcagcgtca gcagaatgac aatcgctttc tgttcaaacc aggagagcac cagcgacagc
    31561 ggcagatcgt tcacaccaca gcccagtttc tctgccagag tgacagccag aataattgct
    31621 gagtaagcat cgttacattg acctgcatct accagacgcg gcagaccttc gatatcgcca
    31681 aactcaagtt tgttaaagcg atatttacca caggcgaggg tcaggatcag gcagtcatcc
    31741 ggcacgctgg tggcgaaatc ggtgaagtag tggcgctcgc cgcgtgcgcc gtcacagcca
    31801 ccaagcagga agatatgacg cagtttttca cggctaacca gatcaatcag cgtatcggct
    31861 gcaccaagca gcgtctggcg accaaagccc acggtgatca ggtgaggaat ttcgctgtac
    31921 gggaagcccg ccatttgttg cgcctgggcg atgaccgcag agaaatcttc gccatccaga
    31981 tgacgcacgc caggccagcc aacgatgctg cgggtccaga tacgatcgtc ataagcgcct
    32041 acggttgggt cgatgatgca gttcgaggtc atcacgatgg ggccagggaa acgagcgaac
    32101 tccacttgct gattctgcca gccgctgccg tagttaccga ccagatgctt gaatttacgc
    32161 agctccgggt agccatgcgc aggcagcatt tcgccgtggg tgtagacatt aacgcccgtg
    32221 ccttcggtct gttccagcag gttgtagaga tctttgagat cgtgaccgga aatcagaatg
    32281 catttacccg ccgtcgcttt gacgttgacc tgggttggcg tcgggtgacc gtatttaccg
    32341 gtttcgcctg catccagaat gctcatcact ttgaagttca tctggccgat ttccattgaa
    32401 cactcaagaa gcgcgttcat atcggcaggc caggtcccca gccacgccat gattttatgg
    32461 tactgggcat aaatatcgtt gtcgtattga ccgagaacat gcgcgtgttc catataggcc
    32521 gccgcacctt tcaggccata caggcacagc agacgcaggc cgagaatgtt ttcgccaatc
    32581 gccgctttat ctttgttagg ggtaaattct gctgcctgac gttgcagctc gccgagatca
    32641 tcgctcacca gttgcaggtc agccatcggg ttatcgacgc gcgcgttggc atctacagcc
    32701 aggcattgtg ctttcagcgc ctcgcgcaag gcaatcgctt cacgagcgta gccgacaata
    32761 cgcggagaat cgaagttaac gttggtcagg gttgagaaaa aggcacgtgg cgcgaagctg
    32821 tcaacatcgt ggttgatgat gccgtattca cgcgctttta ccgcccaggc agaaagccct
    32881 tgcagcgccg cgatgagtaa atcctgaagg tcagaagttt ccgccgtttt accacacatc
    32941 ccctgcgcgt atgagcagcc gttttctgcc ggagtacgga tagtttgttc acattgcaca
    33001 caaaacatga tcacaccttt taaagttata tttaatatac atgtttaagg gtaagacgct
    33061 taacgcgggg ataaaaggga tttttcatgc aactttaagg gagattgatt tagcgcaatt
    33121 ttggcggcag ggaactaccg ccagagaggt attacgcaga gaaaaaggcg atgaggatcg
    33181 gcactaacag gctaagaata aaaccgtgaa caattgccgc cgggaccata tccagcccgc
    33241 cagtacgttg aagaacgggc agggtgaaat ccattgatgt ggcaccgcat aagcccagtg
    33301 cagtagagcg gctgcggcga atcagcccag ggatcaacat aatagcaatc agttcacggg
    33361 ccagatcatt aaaaaacgcc gcgctcccga ttaccggacc aaaagattcg gtcaataaaa
    33421 taccggaaag agaataccag ccgaaaccgg aggccattgc cagcgcggta ttgatgggga
    33481 gatcaagaat aaaggcgtta attaaaccac caattaatga actgacaacc accaccacgg
    33541 cgacaatcat tccccggcga ttaaggacaa tctgctttaa ggtcatgcca ttattgcgca
    33601 actgaatacc aacgaggaaa agtagcaaaa ttaacgtgta ttcactggct tcggtcgcgt
    33661 gttgtaagaa agccagtcca ctcagaccaa tggcaaaacc aatcactact acgccgcaca
    33721 gttttagcga ctccagcgcc atcgcaatac gcgacgggag tttttcttgc tgatggtggt
    33781 tgcgccacgg caggcctcgc tccagccaca
//
LOCUS       DAJTYL010000036        33571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYL010000036 DAJTYL010000000
VERSION     DAJTYL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.036"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            685..822
                     /locus_tag="OO572_001636"
     CDS             685..822
                     /locus_tag="OO572_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660679.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1422..2201
                     /gene="ipaJ"
                     /locus_tag="OO572_001637"
     CDS             1422..2201
                     /gene="ipaJ"
                     /locus_tag="OO572_001637"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6660680.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3148..4077)
                     /gene="virB"
                     /locus_tag="OO572_001638"
     CDS             complement(3148..4077)
                     /gene="virB"
                     /locus_tag="OO572_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6660681.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4294..4530)
                     /locus_tag="OO572_001639"
     CDS             complement(4294..4530)
                     /locus_tag="OO572_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6660682.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4534..6435)
                     /gene="ipaA"
                     /locus_tag="OO572_001640"
     CDS             complement(4534..6435)
                     /gene="ipaA"
                     /locus_tag="OO572_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6660683.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6444..7442)
                     /gene="ipaD"
                     /locus_tag="OO572_001641"
     CDS             complement(6444..7442)
                     /gene="ipaD"
                     /locus_tag="OO572_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6660684.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7493..8584)
                     /gene="ipaC"
                     /locus_tag="OO572_001642"
     CDS             complement(7493..8584)
                     /gene="ipaC"
                     /locus_tag="OO572_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6660685.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8604..10346)
                     /gene="ipaB"
                     /locus_tag="OO572_001643"
     CDS             complement(8604..10346)
                     /gene="ipaB"
                     /locus_tag="OO572_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6660686.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10352..10819)
                     /gene="ipgC"
                     /locus_tag="OO572_001644"
     CDS             complement(10352..10819)
                     /gene="ipgC"
                     /locus_tag="OO572_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6660687.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10876..11502)
                     /gene="ipgB1"
                     /locus_tag="OO572_001645"
     CDS             complement(10876..11502)
                     /gene="ipgB1"
                     /locus_tag="OO572_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6660688.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11518..11907)
                     /gene="ipgA"
                     /locus_tag="OO572_001646"
     CDS             complement(11518..11907)
                     /gene="ipgA"
                     /locus_tag="OO572_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6660689.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11920..13404)
                     /gene="icsB"
                     /locus_tag="OO572_001647"
     CDS             complement(11920..13404)
                     /gene="icsB"
                     /locus_tag="OO572_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6660690.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13718..15334
                     /locus_tag="OO572_001648"
     CDS             13718..15334
                     /locus_tag="OO572_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6660691.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15344..15706
                     /gene="ipgE"
                     /locus_tag="OO572_001649"
     CDS             15344..15706
                     /gene="ipgE"
                     /locus_tag="OO572_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6660692.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15706..16164
                     /gene="ipgF"
                     /locus_tag="OO572_001650"
     CDS             15706..16164
                     /gene="ipgF"
                     /locus_tag="OO572_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6660693.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16173..17288
                     /gene="mxiG"
                     /locus_tag="OO572_001651"
     CDS             16173..17288
                     /gene="mxiG"
                     /locus_tag="OO572_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6660694.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17296..17547
                     /gene="mxiH"
                     /locus_tag="OO572_001652"
     CDS             17296..17547
                     /gene="mxiH"
                     /locus_tag="OO572_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6660695.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17560..17853
                     /gene="mxiI"
                     /locus_tag="OO572_001653"
     CDS             17560..17853
                     /gene="mxiI"
                     /locus_tag="OO572_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6660696.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17859..18584
                     /gene="mxiJ"
                     /locus_tag="OO572_001654"
     CDS             17859..18584
                     /gene="mxiJ"
                     /locus_tag="OO572_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6660697.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18581..19108
                     /locus_tag="OO572_001655"
     CDS             18581..19108
                     /locus_tag="OO572_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6660698.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19050..19745
                     /gene="mxiN"
                     /locus_tag="OO572_001656"
     CDS             19050..19745
                     /gene="mxiN"
                     /locus_tag="OO572_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6660699.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19738..20145
                     /gene="mxiM"
                     /locus_tag="OO572_001657"
     CDS             19738..20145
                     /gene="mxiM"
                     /locus_tag="OO572_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6660700.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20129..20557
                     /gene="mxiM"
                     /locus_tag="OO572_001658"
     CDS             20129..20557
                     /gene="mxiM"
                     /locus_tag="OO572_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6660701.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20687..21319
                     /gene="mxiE"
                     /locus_tag="OO572_001659"
     CDS             20687..21319
                     /gene="mxiE"
                     /locus_tag="OO572_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6660702.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21306..23006
                     /gene="mxiD"
                     /locus_tag="OO572_001660"
     CDS             21306..23006
                     /gene="mxiD"
                     /locus_tag="OO572_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6660703.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23025..24092
                     /gene="mxiC"
                     /locus_tag="OO572_001661"
     CDS             23025..24092
                     /gene="mxiC"
                     /locus_tag="OO572_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6660704.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24105..26165
                     /gene="mxiA"
                     /locus_tag="OO572_001662"
                     /gene_synonym="virH"
     CDS             24105..26165
                     /gene="mxiA"
                     /locus_tag="OO572_001662"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6660705.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26178..26579
                     /gene="spa15"
                     /locus_tag="OO572_001663"
                     /gene_synonym="spaK"
     CDS             26178..26579
                     /gene="spa15"
                     /locus_tag="OO572_001663"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6660706.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26583..27875
                     /gene="spaL"
                     /locus_tag="OO572_001664"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26583..27875
                     /gene="spaL"
                     /locus_tag="OO572_001664"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6660707.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27968..28306
                     /gene="spa13"
                     /locus_tag="OO572_001665"
                     /gene_synonym="spaM"
     CDS             27968..28306
                     /gene="spa13"
                     /locus_tag="OO572_001665"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6660708.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28293..29171
                     /gene="spa32"
                     /locus_tag="OO572_001666"
     CDS             28293..29171
                     /gene="spa32"
                     /locus_tag="OO572_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6660709.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29165..30046
                     /gene="spa33"
                     /locus_tag="OO572_001667"
                     /gene_synonym="spaO"
     CDS             29165..30046
                     /gene="spa33"
                     /locus_tag="OO572_001667"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6660710.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30051..30701
                     /gene="spa24"
                     /locus_tag="OO572_001668"
                     /gene_synonym="spaP"
     CDS             30051..30701
                     /gene="spa24"
                     /locus_tag="OO572_001668"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6660711.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30716..30976
                     /gene="spaQ"
                     /locus_tag="OO572_001669"
     CDS             30716..30976
                     /gene="spaQ"
                     /locus_tag="OO572_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6660712.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30976..31746
                     /gene="spa29"
                     /locus_tag="OO572_001670"
                     /gene_synonym="spaR"
     CDS             30976..31746
                     /gene="spa29"
                     /locus_tag="OO572_001670"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6660713.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31753..32781
                     /gene="spa40"
                     /locus_tag="OO572_001671"
                     /gene_synonym="spaS"
     CDS             31753..32781
                     /gene="spa40"
                     /locus_tag="OO572_001671"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6660714.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32794..33036
                     /locus_tag="OO572_001672"
     CDS             32794..33036
                     /locus_tag="OO572_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660715.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10472 a         4877 c         6722 g        11500 t
ORIGIN      
        1 aatcgtccgg ctactcattc cctgcttgtg caggatttta atttccataa ctgtctcaaa
       61 agtgaccata agctctcctg aatcaggaga gcagattacc ccctggatct gatttcaggc
      121 gttgggtgtg gatcactatt gcaccgttcg tgacacttgt tgactacctg tgcggcatct
      181 ggtatcaagg tggcgtgaag ccggttcagg ttctcatgcg gaacgttgga acctatatcc
      241 ccccgatacc aaaggcgaaa tgtaagtgac aaaatgcaat gaacacgttc tccgttaact
      301 gaggagcatg gtgataaata aaggtaaatg cagaaggtgt agagtctcgc agatttctga
      361 catcatagag cctaacatta gatgcataac gctacagatt gtagaatctc tacaggtgaa
      421 atatacactg gttggcgttt atgtcgcaaa acatgtcatt caggttcatc tcaccaataa
      481 ggatatgagt gaagtggagg ataagtgagt ctgctgtcag agttttctgg tgtatgtcag
      541 taactgcgaa ccatgtctgc ttgatatggg agcttatgga ggctctctgt acaaggttag
      601 ggagctaaca aagcagggat aaaaagtccg cctgatgcag gcacggttcg ttaaatcttt
      661 tgccattggt aacaaaatgg tgcgatgaat gttcaggcta tctttatctt gatggtggtt
      721 cagctgtttg gtaaagaaat cgctgtaaca atagaagaac tgcaggcagt actggtccca
      781 catcgtttgc tcagtcaact ggtgaagttc cgtagcgcgt aaattaatgc tttgcatgaa
      841 acgttactgg agtggatgaa agaatacctg catatatcat tactgcgttg gattaccagt
      901 acaacagatt gaatgaattg gacatgttga tagacggcat tgaaaagcag cttataagca
      961 tagtgcggca gagttaattg gagagcactt cgccttctga gtattaagta agcatggtgg
     1021 tacctgcgga tactatctat tcactgtgct cggtcggcag ccttactgac caaggagttg
     1081 ggggagttga taacaaagtt gaaaaaacag ctctcaacca aggtggttat tgtaacaata
     1141 gcaaataagc tggtgtgacc tgtatcacaa taaccgatta tggacgtaaa tacgacaaaa
     1201 atatgtcagt atcaggcggt attaattgct gatgctgatg gacattaact cttaataaaa
     1261 gggtggatgc tgaaaggttg ttatggtgac ggacgtgatg acgacggaaa agagtatgag
     1321 gttttggatt taaagttcgt tcggggaata aggacccggt cagctaattc tcatagtagt
     1381 tcgcattgct ggatgcaata ctgtgctgca attactcgtc gatgtcggaa caacggaagc
     1441 cttgcaaacg gggatgtatt catacaggtg ttatgttata tggggtttta ttgcaggggg
     1501 ctataccaag agaatatatg atatctcatc agactgatgt gagggtaaat gagaatagag
     1561 taaacgaaca aggttgtttt ttggcgagga agcagatgta tgacaattct tgtggagctg
     1621 ccagtttact ctgtgctgca aaggaattag gggttgataa aataccacaa tacaaagggt
     1681 ctatgtccga aatgacaagg aagagttctc ttgatttgga taatcgttgt gagcgggatt
     1741 tatacttaat aactagtggt aattataatc cgagaataca caaagataat atcgcggatg
     1801 ccggttattc tatgcctgat aagatagtca tggctacgag gcttcttgga ttgaatgctt
     1861 atgtcgttga agaaagtaac attttttcgc aagtaattag ttttatttat cccgatgcaa
     1921 gggacttatt aattggcatg ggatgtaata tagtacatca acgagatgtg ttatcttcta
     1981 accaaagagt tttggaagct gttgctgtat cctttattgg agttcctgtc ggcctccact
     2041 gggtgttgtg tagacctgat ggaagctata tggatccagc tgtaggagaa aattattctt
     2101 gtttttcaac tatggaatta ggtgcaagaa gaagtaattc taactttata ggatatacta
     2161 aaataggtat ttccatagtt ataactaatg aggctttgta aattgtcgtt ttttatctgt
     2221 gaaggagctt gaatattttg gggtgggggg cataacaaat gatattactg agtacaaatt
     2281 tagctatatt gggcggatta gttgtgtggg aagacgaaat gtttctcttg aaataagtga
     2341 actccctcaa ggaatgattg tgtgcttaaa tatttaagtg ctggtaagtt tgagaaggct
     2401 gacatatttt gcagagcaaa cagtgattga cttggatgat atttttattt tagtcactcg
     2461 ttgtaattgg agtatgcttt attttatttg aaaatagatg tattttatac ccgcaattta
     2521 aactgtaagc cctatgaggg gtatggatat tataagttac tctttgacca agcataactt
     2581 ttggggttag aaaaataaat ctcttgttaa taattaatat gattttaaaa tcaaacttgt
     2641 ttataattat aaaataatgt aagtcggtca ttttatggga agattaggag aataaaacag
     2701 taattatatt tgagaaatct gctaatatat tcttaaagct tttttgatac tgtgaacgaa
     2761 gtctgttaat ggtggatttt ttgtttatca ccatgtaaaa aaaattgagt agtaaaagcg
     2821 ttgactgcta atcctcgctg tttttattga aacgattaaa aatacgctga atattttcgg
     2881 ccagtcactc atattcacat aaaattttga ccatcatgcc gcatccctta gttgcacaat
     2941 tcattttcaa tgatatgaat attactaatt tcaccagata attttctcgt ggtcagcagg
     3001 gtatgagttt tcaccgtaat tttaaggaaa cacagtgcgt ggggattgaa taacagatag
     3061 ccgacggaat taccccaccg gctgaaagtc aagttcaacg aagattttta ttatctgaat
     3121 tgggcagttt acatcagtgt tcgatgttta tgaagacgat agatggcgag aaattatatc
     3181 ccgaatagct tcatctattt ctctctgaac tgttttgttt attttagata atgtaaatat
     3241 tagagttctg tttgttttat tctcttttct ttttatgtaa gtccgtttgt ctttagaaat
     3301 aaataacgag tcaactttta gcgaagggtt ttgctttctg tttttgcttt tctttatgat
     3361 gtttaaaatt tcttttttat atatgtccgg gggcaaattt gtatcaaggt cttttatttc
     3421 ttcctttaat attggtagtg tagaactaag agattcatta gccttttcaa gtcctttata
     3481 ataattgaat aatatcttgt agtcattaaa gttaagttct gacgcgattg gaaatagaga
     3541 tattatttcc tgtggaacgc ttgctgcctg aaaggcacga gtgactttcg cgcgagacag
     3601 attctctttt ttggctatgt ctttatagga catccctgat actttcagaa aattaagacc
     3661 aataccaagt tctcggatgc tatgctcttt tgctgtttgt atatcgtttg ctagttttct
     3721 ggcatcaaga gtagatatat attcttttga atatagaact tcaagatctg ctcctgcata
     3781 tattgcagat gctcttctac gagtgccatc cagaatttca attctaccat caatctccct
     3841 tcctattaca gggaagaatt gttgtagctt tatagttttt atgatatcgg ctaatgattc
     3901 ttctgttagg gattcttgat cgcgtacatt cgttttttgg ttgacgaagg ttaaatcttg
     3961 gatcctttta tgaggaatct tggctttgat aaaggaaact ttattaccag aatggagtgt
     4021 aaactctttc ttttggcctt cctttatact taacaagtcg ttgcacaaat ccaccatatc
     4081 acaccctgtt tattcatatt gatttatatt gacaaaagtt aaatgcagtg tgagcaagat
     4141 tgtatgtaac tgttgatgat agtcaacggt agtcttaaaa attactattt taatagctgg
     4201 attgatgcaa tttatcttta ttggataaaa gaataatatt cttgtggagc tctgatgtga
     4261 gagctccata gatgaaaaaa tgcttatcaa ttactatatt gattttgtac tatttttgac
     4321 tacagaaata agatctccaa tagtaataat gtgttctaat gtagattcat ctatacgtag
     4381 attgaactca aaactaatca tatccagcat ttcaatcaga gaaagagaat cggcagaaag
     4441 atcttcaata aatttagttt cttcagatag tttgctgtac gctataccat agcaaaaggc
     4501 cactattgat aatatttttt cttttatcat attttaatcc ttattgatat tctttaatac
     4561 ttttgatagg gaagtggtta catcttttgc tgctttatat atattattgt tttcagctgt
     4621 aatatcattg ttatttttta aatcagatat atcatctgta agtaattctg cagaggtatc
     4681 gtcaattttt gacaatacct tgctgagggc gctagatact tcttttgcct tttcatagat
     4741 cgcatgattt ttatcaattg tatctgcgtt ggtttctgtt acgtagtttc ttatatttgc
     4801 atctgtaact tttaatttgt tgtttattct atgttctgtt gtggattttg taatgtcctt
     4861 tgatttttcc tgtaactctc gttcttgtgt tcctgattta gttccaatga gagatataag
     4921 attacttagg gagtttgtta ctttttttga agcttcaaat atcgtttccc gtgttaaata
     4981 tgtgtctcca tttcttgata aaaaatcagt ctgaagtact tttgaatcag tgtttgagtt
     5041 gaatttattg atttgcgaag ctactctatg agaaagagaa gcgctacttt tagttgtatc
     5101 tgatgagtta tcattactgc ttgtcactcc ttccgggtgg agaggggggg tggttgaaga
     5161 gttctgtatg ttcagaggcc tgtgtccccg agaaagagag ttatttgcag atctttcttg
     5221 actactcgta gttaacaggt ctgaacctat tccatccgaa ttatctggag tatggttgaa
     5281 agccttttct aaagctgaat tatttgcaaa tgtctggaca gtatctgata tagagtgtgg
     5341 tagaacggaa gttacttttt gaactagtgc tctttgtgga tttagtaatg agtttccgtt
     5401 acgggataat agatcgtttg tctgagaagt aggagactgt gcatcatttt ctgggttctc
     5461 gtgatagcta ttgtcatata ctctgttgtc aaaaactcta ttatcgtaag ttctattatc
     5521 attatttata tgatagtgaa ttacaggttg ggatgttttc tcattggctc cgggggttgg
     5581 tccgccatct ggcatatctt cgggaggtgt agggaggcca tcttggatag gatgcgaatc
     5641 ttttatatct tctaaaatat cattaaggac ttttctcaac agctcaggaa acacggctac
     5701 tgtatttaag tctcttgaca gtttacctat accaaatcct ttactatcaa tagtattttt
     5761 acgttgattg cgcaaactat caaaggcaag ttttgcctgt gcgtcaacta aatcatctag
     5821 tttgagagat gaaacagttt cattacttaa ctttaattca gcaagaatat tattcttgat
     5881 aaatgatgat acttcagtat tgaggagttt ttgtcccaat gataaagctt caccagatgt
     5941 gtgttgttgt gctaatagtg caagtctgtg ctcaatgttt tgtcgtaatt gatggagttt
     6001 cttattcttt gccgaccatg gtccaaaggc agaatcttgg gataatttat cttttataat
     6061 tagttccgaa tactgtgcga caattttttg aagtcccttt tcgttttcaa aattaagacc
     6121 attttgatgt actaaggagt acaaaacatt tgaaaattcg agaatctcgc ttttggcata
     6181 cgcattcatt gagttggaat aaatgcttct taacacatct tctttccctg aggaagaaaa
     6241 taaaaaatca agacatttct gattaaaaga cgttgcaaag ttttcttttt cagacactga
     6301 tgctggtgtt tttagagaag tttgcgaact atggatatcg gatattttgc tttttaactc
     6361 gctgtattct gttgatgatg gtgaagttgc cttatataag aatgttggcg cttgagtatt
     6421 atttacatta tgcatggcgc acctcagaaa tggagaaaaa gtttatctgt atctgtacat
     6481 gagcttattg tactactcaa aacctttact aaattatcaa aaatactatt ggcattactg
     6541 tatttttgaa ctaaagtttg aagattattt ttcattgttt catcttcggc agagaatccg
     6601 gcattccatg cctgatattt tgcattatct agcacaacct cgccatttcc acctagatta
     6661 tctaagcttt ttaacatatt gtctattggg gtcatgttta tactgacaac atatcccccg
     6721 tttttttgag ataccttgcc gattgttcca cctaattctg taagccattt atttgcttgt
     6781 tcctgactaa cagtattatt tgctggatat agcggtttat ctttatattt ttccttgagt
     6841 tcttccaatg cctttttaag cgagttgact tgtaatttca cggagtttcc gtcgttacct
     6901 ccgggagaga tccagccggc aagactggaa agaacagcgc taaaatcttg atacatttga
     6961 gtatatgaac taacggcatg ttcatatact ttcagatact gttcattaat atcattgatg
     7021 gagtttgcaa ttttagccca cagttctcta tgagatatca tttgatctcc atcaagctcg
     7081 gcttctttcg gagctgaatg taataattct cttgcgtctt tattaattgg atattcgtgc
     7141 ctggaaagaa tatctaatag ttgagcggat ttatttacat ccatgctaat ctgagatgaa
     7201 tgtaatgcta tttcttctag cgatgtttta gtcaacgttt tttgtgaaag ctctttcttt
     7261 aatgcctgat ttgttgttct gatattatga agggtgtcgt tgagcatagt aagggaactt
     7321 acaggatgag aactggtcgt tgtttttata tcagaattaa ctgtttcggt tgatgaaccg
     7381 ttggtattgt ttggactgaa tgatgaggtg gaaatactat tagtcagagt tgttatattc
     7441 atggttattt ccttacagga agagccatat attattatgg ctcttggata tattaagctc
     7501 gaatgttacc agcaatctga ctggctgtcg aattctttga ttggttgatg ctgtcaatta
     7561 tattcaataa tttttgtatt aattgatttg tcgcttggga tgcttcttta gatacttggg
     7621 atgcttcctc tgcttgttta ctgctggcct gactgattag ttgttcttct tcttcaagag
     7681 cagatgcata acgccctcct gatgtggata tattaccagc aacggctgat gtttccattg
     7741 ttgcacggcc aatgttttgt ttttgctgcg cagaaagggt attgagctcg taagttcttc
     7801 tctgggtgtc aattttatcc tgcaaagaaa tatcgggaga actaagagaa gttttatgtt
     7861 cagttgacag ggatatatta tctggcgcca gtttattttt acctaaaaat tttgtgctag
     7921 agttagtttg tggtgaggtg atatttgtat ctatttgttt atttaaccct aatttagaac
     7981 ctgcaagctc tttttcaaga gattgagcag tggcaaggtt ctttcttaag gctccttttt
     8041 ggtcgctaat ccctgaatgc gtttttttgg caccgatacc cgttatacct acttgtgtga
     8101 ctgctccagt aatgcttgat gatagggctg ccaggccttg ccgaacaatg ttctctgcag
     8161 ctgattttgt agcatcgaac gcaatcaatg acaattgaga gcccaattta gtttctgcag
     8221 tgcggagagc agaaagtaga acggctacac taataattaa agaaacagca ttagaagaaa
     8281 gactggaaat atccagagtg ttctctggag taagggtgtg actcatctct gaaagatctt
     8341 tcatcttttt gtcagtaagg cgagcaatga tttctgaatc atgctgaacc tgctctgata
     8401 actttaaaag ttgatcatca tttaatgtgg ttgttaatac gggattttta ccgacattaa
     8461 gtggaatatg cgctgcaatc tgctgataat tttgtgattt ttgtgtttcg gaagaacttt
     8521 gagtttgttt tgtggatata tctgtatata aaatctgggt tggttttgtg ttttgaattt
     8581 ccataacatt ctccttattt gtatcaagca gtagtttgtt gcaaaattgc ttttgcaaca
     8641 tcagttctat tagcctgaga gttggacatt gaggctaata gatctgcaat tacttcctgc
     8701 aattggccga atttttctat tgcttcactg atatattttg acagttgttc aacttgatat
     8761 ttcgataatg tcaggtctgc tagatttgtc gacgcgctgt tctggaaaac agcagaagcg
     8821 acacttcctc ccgcttgtgt ggcagagtta acactttctc ctaataaaac tgcttggttt
     8881 aaatgggtgg aaataatttg tttggatatt acttcatcac ccgctgcacc aagaaattta
     8941 tttaatctgg caacagcatt agtgattaaa tcgtccagtt gagaagaaaa attcttgaga
     9001 aactttggta taaggtctgt gagggtttta cctattattt tgccaatatt ttctgcaagt
     9061 tttgctgctg cctgtttacc aacagtggct acgagaacga ctgctgcaac taggacaaga
     9121 gcgcctgcga ttgcccccag aatagagcca atcattttgg cttttttcga gtcgacgccc
     9181 aagccttcga gcatttttgt aaatgcatct gaaaggagtt tgattaaggg ttcaatgact
     9241 gctttcatga tcggattcag ggcttgttcc atgaaggaat tgccggtcgc tgcttgtact
     9301 atagcatccg taaccataag agctaaacca acagctgcca gtgctagaga ggctcctcca
     9361 gaaaaagctg ctgcaacaac actaacgata gttaaaagtg ccccaagtat tttcccaaca
     9421 caacccatta ctctgttgag ttcttctgct ttacgtactt cagcagcata ctcatcagat
     9481 tttctctcca tttcagtttt tcttgattct tggagagact ggaatagagc cagatcattt
     9541 tttaaagatt cttcattatt ttttccaact agttgaataa aggttgccat caattgagta
     9601 acactggcaa gtccggttaa tgatttctgc tgggttgata gctgttcagc agatgctgtg
     9661 tttgaaaatg cagaaaaaga gtctatttct ttttcgagtt gcattgattt atctgtaagt
     9721 ttgctatgaa ttgacagggt tttctgctca atcaatgtcc tgtctttaac tgctgcgtct
     9781 tttttgatag tgagttgtat ttcttcccgg cttaattttt tcttttctgg tgactctggg
     9841 tcgagttcgg ataatcttgt ttgaatttgg ttaattttat tttctaggtc ttttatttta
     9901 gaatctgcgt tttttagttt attaatttgt ttttcatagt ctctggttag tccttcagtt
     9961 tcagatagaa gagtgttaat tttatcggag aattctaggt ttttttgctg tcttgcctgt
    10021 tgctgggact tccaagcagt aattttattt gttaatgcag ttaaagattt ttcaccgagt
    10081 atttgaataa ggtttccaat taaaagcgtt agttgggaac ttgcatttaa tgactttggt
    10141 gctttaagtt cagggattaa tatatttgaa gttgagtgtg cattagttgt attaatattt
    10201 tggttagcag taagatcagc aattgtaaga gaaaataatt tgttagctgc atcatttgca
    10261 gcttggatag tattgtctcc aagctcagtg gaagccaata ttttggcaag aggaaaacca
    10321 gtggttgtgg tgcttacatt atgcataata attactcctt gatatcctga attgcgtcca
    10381 agtatgattg tgcttttatt tttaattttt catcattgct gtgttgaatt acgagttcga
    10441 agcactcttt agcttttaag ggggctttca accgaagctg gcattgtcca gtatggaata
    10501 ctggtgtata gtcatttttt cctaatgcaa aagcgacagc ataaaggtct gctgcttgtt
    10561 ggaactgttc ttttatctga taaatagctg cgagtcccat aatgtagtct acattgtaaa
    10621 agtcgtatat acataaaaac ctgaagaaaa cttcagcttc ctctattctt cctttgttgt
    10681 aaaagtcata agcatatgaa taaatgtcat ccatcatatc atcaggaatt gcattaatat
    10741 ctttcagtgt agcgccagag ttaattgcat caattactgc agtagagatg ctttcatttt
    10801 cggtgatatt taaagacata aggtctcctt tttgtctgct ttactttaat aagtataaga
    10861 tttaatataa aagatttaat ttgtattgct ttgacggtat acagccttta gaacctctgc
    10921 gcttatttct atttgtaaaa cttttgttga ttcattaaat attttatcgt agacggaact
    10981 ggatactaga tttgtaagat ccttcatttg tgtgttaaac aaatccatgt cactgctata
    11041 gcgttttaaa atctttgctc tgaggccaga tgaggcaatc tcgtttagtg ctgtatgact
    11101 gatagatgtt tgtgctgatt tttcatcaag ttttatatcc gggaattttt tttcaatagc
    11161 tgcaaatatg ttttttttgt tatctgaggt caaaacaact ccattgtgta tggccatttc
    11221 atctatactt ctgtttatca ttgaagagac ataagtggtt cgctcttggc tcatccaaca
    11281 aaatagttga ttccccgaat tactatcttt tattttgtct atgacgctgt ccctgatttt
    11341 atttatattc accgggcatc caaaattctt ctgaggaaag cgttgtttta agttaaaaac
    11401 agataatatt ttctttacaa gcttattgcg acttaacgaa tcaaatgatg ggcgaggaat
    11461 cgcgaattca acctgtggaa gtattttgtt tagaatttgc atgatacccc ctatatgtta
    11521 gttcacttct gaagtgatgt ttgcaatggt tagtttaata caaaataaaa attcatacaa
    11581 agattcaatg agttcatcag gtgaaagata ttttttgtag tgtttcttgg tcaaaacaca
    11641 ttgatgagta tcagggagaa gacagataga agaaccataa tctctactca aagagtagtt
    11701 aaggatgagt atgtgttctg gattaataat attttctgta aaaagttcat gttcattaag
    11761 aagtccggtc aaaaagatgt attcatcatt atctgttata caaacaggta tttgttcttc
    11821 gagcaatata aaggcttgat ttttatcgtt aaaatcaagc tttgcacctg ttatttcata
    11881 aagtttatca tatagtttgc gacacatttc tatggccttc tatatattag aatgagagtt
    11941 attcaataaa agcttattga tctgtaataa gtcgtgaatg taaagtttgg ttccaagatc
    12001 tggcgattta agagaattgt aataatcaat aatttttatt gatttttcga tgatttcttc
    12061 ttttttatct gttgcttcgg tttttaattc gtttaataca cgagtcatat tgctgggtgc
    12121 tgattttact aagtatgagt ggcaagcgtt gatgaccgca tcaaagtctt ctgaagatac
    12181 attttcagta ggatgtttgt aaagtgaaat tgataaaggc ttgaatgttt ttggtgtgtg
    12241 gataaggtta attttattga aacttttaag atagctgcgt cgtaattgag tcaatctgga
    12301 aagatcaaaa tcagattcaa tatacttcat tatttcgtta taagactgat tgagactttc
    12361 aatgcgttgc ctaactttat ttgcatacgc gtatacatcg ttaggtgtag caaccaattt
    12421 aacgtcaggg agtggaaagt atatttcaga atttcctgct ttaagaacat taagtgccat
    12481 accagcacag ttttcagatt tactgattaa tttataattt gccaattgtc tgaaagcatc
    12541 tgctttcttt ttgcatataa attttgacat gtttgtttca tcaaggccaa aaaggttatg
    12601 gctgatttta ccatcctttg ttttattatc acctgataat ggaatgtata ctttatcagc
    12661 actgaccccc caataaccat aggggtcttt tttttgatta gctctgggaa aaaatatttt
    12721 tttgttgtta ttttgatctt gttttgaatt taataagttt ctaacctctt ggcctgcatc
    12781 aagtctttcg gctgtttggt ctgaaatcat atttagtttg tcttgcttat aggtatcgac
    12841 actgtaacct gagcgttttt caaaaaggta gtttatttct gtatcttttg taacattccc
    12901 gccgggatac cagctgagat aggtatgagt tggttcctgt agtaaattgt tttttatact
    12961 gatactagta tgccctaaat gggttatgcc tctatttccc cagcaacgaa aaactaaatg
    13021 tctttcaaga tttttggctg taccgatgcc atgaaaacgc tctgcggtat tcaacattaa
    13081 tttgatgtct ctacctgtga aagagtgttg aattgagttt ttagctgctt cataggcggc
    13141 aaacgcactt tctgcagatt tttcgctagc taatgtattg aggaaaagca ataaggattt
    13201 ttgattctct agtcgataat tttttagttg ttcatttaat atctgtgaat ttatctttgc
    13261 attaatagtg cttaatgttc tgaaaaagag atcctttagg ttgaatttct gtgctatcaa
    13321 tataggtcca tgctctgtat cttcaacgcg tataggccct tttgtattgc ttgcgtcaat
    13381 gaaattgcta attttgagga tcatacttta ttaactctcc attacttggt gattgtgggg
    13441 atgtaaaatg tatttggtat ataatatatt caattaataa atttagaaac ttgagcctgt
    13501 taacataatc aaattttctt ttgctgtaca taatatgtac ctcgtgagca tatgtagtgc
    13561 tcgtttcatc atgaaatccc acaagataaa gtgcctgatg tatcaggctc ggagtgttat
    13621 agaaaaagag agaaccctgt tgaataagta tcttttttgt tgtaaaacga ggtacaaaac
    13681 tgcaatcaat gccccattta tgactgagga taattaaatg cacataacta atttgggatt
    13741 gcatcaggtt tcatttcaaa gcggagattc ctataaaggc gcagaagaaa ccggaaagca
    13801 caaaggtgta agcgtgattt catatcaaag agttaaaaat ggagaaagaa ataaggggat
    13861 cgaagcgctt aatcgattat atttacagaa ccaaacaagc ttaacaggaa aaagcctttt
    13921 gtttgcgcgt gacagagctg aagttttttg cgaggcaata aagttggcag gtggtgatac
    13981 gtcaaaaatt aaagccatga tggaacgatt agatacctat aaacttggtg aagttaataa
    14041 aagacatatt aatgagctta ataaagtaat aagtgaagag ataagagcac agctaggcat
    14101 taaaaataaa aaggaattgc aaacgaaaat aaaacaaata tttactgatt atttaaataa
    14161 taagaattgg ggaccggtcg acaagaatat tagtcatcat gggaaaaatt atggttttca
    14221 attaactcct gcctctcata tgaagatagg gaataaaaat atatttgtca aagagtataa
    14281 tggaaaagga atttgttgtg cttctacgag agagagtgat catatcgcga atatgtggct
    14341 ttcaaaagtg gtggatgatg aggggaagga aattttttcg ggtatcagac atggagttat
    14401 atcagcctat ggattgaaaa aaaactcatc tgaaagagct gttgctgctc gtaataaagc
    14461 tgaggagtta gtaagcgcag cattatatag cagaccagag ttattatcac aggctttgtc
    14521 tggtaaaaca gtagatttaa agattgtttc gacttctctt ctgacgccga ccagtttaac
    14581 cgggggagag gagagtatgt taaaagatca ggtgaacgca ttaaaagggt taaactctaa
    14641 aagggggaag ccaacgaaat tattaattcg gaatagtgat ggtcttctga aagaagtaag
    14701 cgttaatcta aaagtggtga catttaactt tggtgtaaat gaattagcgc taaaaatggg
    14761 cttaggctgg aggaatgttg acaagcttaa tgatgaatca atatgttctt tgttggggga
    14821 taatttcctc aaaaatggtg tgattggagg ttgggctgca gaagcaatag aaaaaaatcc
    14881 accatgtaag aatgatgtga tatatctggc taaccagata aaagagattg taaccaaaaa
    14941 gttacaaaaa aatgataatg gagagccgta taaattgtca caaagaatga ctctattggc
    15001 ttatactata ggtgctgtac cttgctggaa ttgtaagagt gggaaggaca gaacaggtat
    15061 gcaggatgct gaaattaaaa gagaaataat aagaaaacat gaaacaggtc agttttctca
    15121 attaaatagt aaattatcct cagaagagaa aagattattt tctactattc taatgaatag
    15181 tggtaatatg gaaatccaag agatgaatac tggtgtgccc ggaaataaag tcatgaaaaa
    15241 attgccgcta tcttcgttag agctatctta ttctgaaaga ataggggacc caaaaatatg
    15301 gaatatggtg aaagggtatt cgtcatttgt ataagaggaa tatatggaag atttagcaga
    15361 tgttatttgc cgagccttgg gtatcccttt aattgatatc gatgatcaag caatcatgct
    15421 tgatgatgat gtgctaatat atattgaaaa agaaggagat tcaataaatt tattatgccc
    15481 tttttgtgca ttgcctgaaa atataaatga tcttatatat gcgttgagcc taaattactc
    15541 agaaaagata tgcttagcta ctgatgatga aggcggaaat ctaattgcgc gattagatct
    15601 taccgggata aatgaattcg aggatgtata tgttaataca gaatattata tttcgcgtgt
    15661 gcgttggctg aaggatgaat ttgcgcgaag aatgaagggg tattaatgtc ccgtttcgta
    15721 tttattttgc tgtgttttat cccatattta gggagagctg attgttggga taaggctggt
    15781 gaaaggtaca atattccatc aagtttgtta aaagcgattg cggaaaaaga atccggattt
    15841 aataaatctg cagtcaatgt taataacaat ggaagtaaag attatggtat aatgcaaata
    15901 aatgactttc attctaaaag acttagagaa atgggatatt ctgaggaaat gctaattagt
    15961 catccatgtc tttctgtaca ttatgcagca aagttattaa atgagtttat gatgatgtat
    16021 ggaagagggt gggaggctgt tggagcctat aatgcaggaa cgtctccgaa aaagaaaaaa
    16081 gaaagattaa agtacgcaga ggatatctat agacgctatt tgcggatagc agcagagagc
    16141 aagcagaata atcgaaggat ataagaggat ttatgtctga ggcaaagaac tcaaatcttg
    16201 caccattcag gttattagtg aaactcacaa acggtgtcgg ggatgagttt ccgttatatt
    16261 atgggaataa cttaattgtt ctcggacgga cgatagaaac acttgagttt ggaaacgaca
    16321 atttcccaga aaatattatt cctgtaacag atagtaaatc ggatggtata atttatttga
    16381 ccataagcaa agataatatc tgtcaattta gtgatgaaaa aggtgagcaa attgatatca
    16441 attcacaatt taattcattt gaatatgacg ggatttcttt tcaccttaaa aatatgaggg
    16501 aggataaatc gagaggccat atactaaacg gaatgtataa gaaccactcg gtgttttttt
    16561 tctttgctgt tattgttgtg ttaattataa ttttttccct ctctttaaag aaagatgaag
    16621 taaaagaaat tgctgaaatt atagatgata agagatatgg aatagtcaac actggacagt
    16681 gcaattatat tcttgccgaa acacaaaatg atgcggtatg ggcgtctgta gccttaaata
    16741 aaaccggatt taccaaatgc agatatattc tggtgtcaaa caaagaaata aatagaattc
    16801 aacaatatat caatcaacga ttcccattta tcaatttgta tgtgttgaat ttagtgagtg
    16861 ataaagcgga attactggtg tttttaagta aagaaagaaa tagttcaaaa gataccgaac
    16921 ttgataaatt gaaaaatgct ctgattgttg aatttccgta tatcaaaaat attaaattca
    16981 actatcttag cgatcataat gcaagggggg atgctaaagg tattttcaca aaggtaaatg
    17041 tccagtataa agagatttgt gaaaataata aagttacata ttctgtcaga gaagaactga
    17101 cagatgaaaa gcttgagctc attaatagac tgatttctga acataaaaat atatatggag
    17161 atcagtatat tgagttttct gtacttttga tagatgatga ttttaaaggt aaatcatatc
    17221 ttaacagcaa agacagttat gtaatgttga atgataaaca ctggtttttt ttagataaaa
    17281 ataagtgagg ataaaatgag tgttacagta ccgaatgatg attggacatt gagttcatta
    17341 tctgaaactt ttgatgatgg aactcaaaca ttacaaggtg aactaacatt ggcactagat
    17401 aaattagcta aaaatccttc gaatccacag ttgctggctg aataccaaag taaattatct
    17461 gaatatacat tatataggaa cgcgcaatcc aatacagtga aagtgattaa ggatgttgat
    17521 gctgcaatta ttcaaaactt cagataatag ggagcattca tgaattacat ttatccagtc
    17581 aatcaggttg atattatcaa agccagtgat tttcaatctc aagagatatc aagtctggaa
    17641 gacgtcgtgt cggctaaata tagtgatatt aagatggata cagatattca agtatcacaa
    17701 ataatggaga tggtaagcaa tccagaatca ttaaacccag aatctttggc caagttacag
    17761 acgacgctct caaattattc aataggagta tcattagctg gcacgttagc aagaaaaaca
    17821 gtttcggctg ttgaaacttt attaaagtct taatttatat gattaggtat aaaggtttta
    17881 ttttattctt gttgctgatg ttgattggat gtgagcaacg tgaagagtta atttctaatt
    17941 tatctcaaag acaggcaaat gaaataatat ctgtgctaga acgccataat attactgcta
    18001 gaaaagttga tggaggtaaa caggggatct cggtacaagt cgaaaagggg acatttgcat
    18061 cggcagttga tttgatgcgc atgtacgatt tgccaaatcc ggagagagtt gatatctcac
    18121 aaatgtttcc tacagattca ttagtgtctt ctccaagagc tgaaaaggcc cgtttatata
    18181 gtgctattga gcaacggctg gaacagtctt tagtttctat tggtggtgtt atttcggcaa
    18241 aaatacatgt tagctatgat cttgaagaaa aaaatatatc ttcaaaaccg atgcatatat
    18301 cagtaatcgc tatatatgac tcaccgaaag agtctgaact attagttagt aatattaagc
    18361 gatttttgaa aaacaccttt tctgatgtta agtatgaaaa tatatctgtc atattaactc
    18421 cgaaagaaga atatgtttat acaaatgtac aacctgttaa ggaagttaaa tcggaatttt
    18481 taacaaatga agtaatatat ttatttctcg ggatggctgt actagttgtc attcttttgg
    18541 tatgggcatt caaaacaggg tggttcaaga gaaacaaaat atgataagaa tggatggaat
    18601 ttataaaaaa tatctttcaa taatttttga tccagcgttc tatataaata gaaatcggtt
    18661 gaatttgcct tctgaactgt tagaaaatgg cgtaatcaga agtgagatta ataatctcat
    18721 aattaataaa tatgatctaa attgcgatat tgaaccttta agcggggtaa ccgctatgtt
    18781 tgttgccaac tggaatttac ttccagctgt tgcttatttt atcggtagtc aagagtctcg
    18841 gttaattaat catagtgaga tggttattag ttattatggg ggcaaaatca gtaagcaagg
    18901 ggaggccgct attcgaagtg gtttttggca tcttattgcg tggaaggaaa atatatctgt
    18961 tggtatatat gaaagaataa acttattatt taatccaatt gctttggagg gaaattatac
    19021 gcctgtagaa agaaatttgt cccgattaaa tgaaggtatg caatatgcaa aaaggcactt
    19081 taccggtatc cagacatcat gcctatgatg gtgttgttat aaagagaatt gaaaaggaat
    19141 tatgtaaaac cataaaagat agagacactg agagtaagaa gaaagcaatt tgtgttataa
    19201 aggaggctac gaaaaaagct gaatcactgc gtatagatgc tgtatgtgat ggttatcaaa
    19261 ttggtataca gactgctttt gagcatatta ttgactatat ttgtgaatgg aagttaaagc
    19321 aaaatgaaaa tagaagaaat attgaggact atataactag tttgctttca gaaaatttac
    19381 atgatgagag gatcattagc actttattgg agcagtggct tagttcgttg cgtaatacag
    19441 taacagaatt aaaagtggtt ttgcccaagt gtaatttagc actgaggaaa aagttagaac
    19501 ttgatctcca taaatatcgc tcagatgtca agattatttt aaaatattct gaagggaata
    19561 attatatatt ctgtagtggt aatcaagtag tggagtttag tcctcaggat gtaatatcag
    19621 gtgttaaaat agaacttgcg gaaaaattaa caaaaaatga taaaaaatat ttcaaagagt
    19681 tagcacataa gaaactgaga caaattgcag aagacttgtt aaaagagaat cctgttaatg
    19741 attaatcaaa taaatgcaag caatgctctg cagcaaagat taaatagtga agaagttgtt
    19801 aatttaaatg agagattatc atcttcgcaa tcatttgatg aggatataat ctatgaaata
    19861 atgcaatatt tctcacagag tgaattaaat tccatagata atgatgaact gcacaataag
    19921 attgaacaat tattcaattc acgttttcct tatcttacag ctgcacaaaa aagttcactt
    19981 cttaataaac tcatagatgc aaatcaatat gtcgatttgc atgagggatt ttatgcatca
    20041 ttgtccatat ataacaatat tgatttctat attaagacta caaccttcga ttcattaatt
    20101 agtgtctttg aagcagggag agaggcagat gattcgacat ggtagtaata agttgaaaat
    20161 atttatttta agtatattgc tattaacact gagtgggtgt gctttaaagt catcatctaa
    20221 ttctgaaaaa gaatggcata ttgttcctgt aagtaaggat tatttttcta ttccaaatga
    20281 tttattatgg tcgtttaata caaccaataa aagtataaat gtttactcta aatgtattag
    20341 tggtaaggcg gtttatagtt ttaatgcagg taaattcatg ggcaacttta atgttaagga
    20401 agtagatggg tgcttcatgg atgcacaaaa gatagctata gataaactat tttctatgct
    20461 gaaagacggg gttgttttaa aaggtaataa gataaatgat accatcctta tagagaagga
    20521 tggggaagtt aaattaaaat taattcgagg gatataattg tattgtgagt aaatataaag
    20581 gtctaaatac aagtaatatg ttttacattt actctagtgg acatgaacca gttaacgttg
    20641 agcttgtaaa agataaagaa cgtaacataa ttgagctggc tccagcatgg aagggttttt
    20701 ttttgtccga aatcaaaata taaaattcag tgataatgtg aactatcatt atcgctttaa
    20761 cattaatagt tgtgcaaaat ttcttgcttt ttgggactat ttcagtggtg ctctagtaga
    20821 gcatagccat gctgaaaaat gtattcattt ctatcatgag aatgatcttc gggatagttg
    20881 taataccgaa tcgatgcttg ataagttaat gttaagattc atcttttcat ctgaccagaa
    20941 tgtaagtaat gcactggcta tgatacgaat gactgagtca tatcatttgg ttctttatct
    21001 tctgagaact attgagaaag aaaaggaggt aagaataaag agtctgacag agcattatgg
    21061 tgtgtcggag gcttatttta gatctttatg tagaaaagct ttaggggcaa aagtcaaaga
    21121 acaactgaac acttggcggt tagtaaatgg tttattagat gtatttttgc acaatcaaac
    21181 aatcacttca gctgccatga ataatgggta tgcatcaaca tcccattttt caaatgagat
    21241 aaaaacacga ctaggctttt ctgcaagaga attatcgaat attacttttt tagtgaaaaa
    21301 aataaatgaa aaaatttaat attaaatctt tgactctctt gattgtattg ttaccattga
    21361 tagttaatgc taataatatt gactctcatt tattagaaca aaatgacata gcgaaatatg
    21421 ttgctcaaag tgatacggta ggatcttttt ttgaacgttt ttctgcgtta ttaaattatc
    21481 ctattgttgt tagcaaacaa gctgcaaaaa agagaatatc tggggagttt gatttatcta
    21541 atccagaaga aatgcttgaa aaactgacat tacttgtggg actcatatgg tataaagatg
    21601 gaaatgcatt atacatatat gactctggag aattaataag caaagttatt ttgctggaga
    21661 atatatcact taattatctt attcagtatc tgaaggatgc taacctttat gatcacagat
    21721 atcctataag aggaaacatt agtgataaaa cattctatat atctggtcct ccggcattgg
    21781 ttgaactggt agctaataca gcaactttgc ttgataagca ggtttcttct attgggacag
    21841 ataaagttaa ttttggggtt ataaaattaa aaaatacatt tgttagtgac agaacttata
    21901 atatgagagg ggaggatatt gtcatcccag gggtggctac tgttgtagaa cgtttattaa
    21961 ataatggtaa ggctctgagt aacagacaag cccaaaatga tccgatgcca ccctttaata
    22021 ttacacagaa agtatctgag gatagtaatg acttttcatt tagttcagtt accaatagtt
    22081 ctatattaga ggatgtatca cttattgcat atccagaaac caattctata ttagtcaaag
    22141 gtaatgatca gcaaatacag attattcgcg atattatcac tcagttagat atagccaaac
    22201 gacatataga attatcatta tggataatcg atattgataa atctgaatta aataaccttg
    22261 gtgttaattg gcaaggaacg gcttcatttg gtgattcgtt tggtgcgtca tttaatatga
    22321 gctcatcagc atcgataagc actttggacg gcaataagtt tattgcttca gttatggcat
    22381 taaatcagaa aaagaaagca aatgtagttt caaggccggt gattttgaca caagagaata
    22441 ttccggctat atttgataat aacaggactt tttatgtgtc attagttggt gagcgcaatt
    22501 cttcgttgga gcatgttacg tatggaacat tgataaatgt tatacctcgc ttttcatctc
    22561 ggggacaaat tgagatgtct ttaacgatag aggatggaac aggaaatagt cagtcaaatt
    22621 acaattataa taatgaaaat acatctgtcc ttccagaagt tggaaggacc aaaatcagta
    22681 caatagcgag agttccacaa ggcaaaagtt tgttgatagg tggttatacg catgaaacga
    22741 actctaacga aataatatct attccttttc tctcctctat tcctgtaatc ggtaatgtgt
    22801 tcaagtataa aacaagtaat atcagtaata ttgtaagggt ttttctgatt caaccccgtg
    22861 agataaagga gtcaagttat tataatactg cagaatataa aagcctcata tctgaaagag
    22921 aaatacaaaa gactacacaa ataatcccat ctgaaactac tttgctggaa gacgaaaaat
    22981 cattggtttc atacttaaat tactaactat aaagtaggtg atgtatgctt gatgttaaaa
    23041 atacaggagt ttttagctct gcattcattg ataagttgaa tgcaatgaca aattcagatg
    23101 atggagatga gactgctgat gcagagcttg attctggctt ggctaatagc aagtatattg
    23161 actcatctga tgagatggct tccgctcttt cgtcatttat aaacagaaga gaccttgaga
    23221 aactgaaagg aacaaatagt gatagtcagg aacgtatttt agatggggaa gaagatgaaa
    23281 ttaatcacaa gatttttgat ttaaagagaa cgttaaaaga taaccttcct ctagatcggg
    23341 attttataga cagactaaag agatatttta aagatccaag tgatcaagtc ttagcattaa
    23401 gggaactttt gaatgaaaaa gatcttactg ctgaacaagt cgaattatta actaaaatta
    23461 ttaatgagat aatatcaggt agtgaaaaaa gtgttaatgc tggaataaat tcagctatac
    23521 aggctaaatt atttggcaac aaaatgaaac ttgaaccaca gcttttgcgt gcatgttatc
    23581 gtggttttat catggggaac atatcaacaa cagatcagta tatagaatgg cttggtaatt
    23641 ttggttttaa tcacagacat acaattgtga attttgtaga gcagtcactg attgtagaca
    23701 tggattctga gaaaccgagc tgtaatgctt atgagtttgg ttttgtgtta tctaaattaa
    23761 ttgcaattaa gatgattaga acttcagacg taatttttat gaagaaactg gaatcctcaa
    23821 gcttgctaaa agatggcagt ttaagtgcag agcagctatt gctaacttta ttatatattt
    23881 ttcaatatcc aagtgaaagt gagcaaattc ttacttctgt tatagaagta tcacgagcca
    23941 gtcatgagga ttctgtagtg tatcaaacat atctatcttc tgttaatgaa agtcctcatg
    24001 atatatttaa aagtgaaagt gaaagagaaa ttgcgatcaa tattctacga gagcttgtca
    24061 caagtgcata caagaaagag ctttctagat aacaggagat aaaagtgatc cagtcttttc
    24121 ttaagcaagt aagtactaag cctgaattaa ttatattagt gcttatggtt atgatcattg
    24181 ctatgttgat cataccatta cctacatacc ttgttgattt tttaataggg cttaatattg
    24241 tacttgctat tctcgttttt atgggatcat tttatattga aagaatattg agtttttcta
    24301 cattcccttc ggttttgttg ataacgacat tatttcgtct cgctttatca ataagtacta
    24361 gtcgactaat tttagttgat gcggatgcgg gtaaaattat tactacattt ggccagtttg
    24421 tcattggtga tagtctggct gtaggttttg ttattttttc aatagtaact gttgtgcagt
    24481 tcattgttat aacaaaagga tctgaaaggg ttgcggaggt agctgctcgc ttttctcttg
    24541 acggtatgcc ggggaaacaa atgagcattg atgcggattt aaaggctgga attattgatg
    24601 ccgcaggagc taaagaaaga cgcagtattc tggaacgtga gagtcaattg tatggttcat
    24661 ttgatggagc aatgaagttc attaaaggtg atgcaattgc tggcatcatt attatttttg
    24721 tcaatttaat aggaggtatt tctgttggta tgagtcaaca tggaatgtcc ctatctggtg
    24781 cattatctac ttataccata ctaactattg gtgacggatt ggtctctcag attcctgctc
    24841 tgttaatttc tataagtgca ggatttatcg ttacaagagt taatggtgat agcgataata
    24901 tgggacgtaa cataatgtcc cagatatttg ggaatccttt tgttctcatt gttacatcag
    24961 ctcttgcctt ggcgataggg atgttgccag gttttccgtt ttttgttttt ttcctgatag
    25021 cagttacttt gacggcttta ttctattata aaaaggtcgt agaaaaagaa aaaagtctgt
    25081 ctgagtctga ttctagtggc tatactggta catttgatat tgataatagc catgactcat
    25141 ctttggcaat gatagaaaat ctggatgcta ttagttcaga aaccgttcct ttaattttat
    25201 tatttgccga aaataagata aatgcaaatg atatggaagg tcttattgaa aggataagaa
    25261 gtcagttctt tattgattat ggtgtcaggc ttccaactat tttatataga acaagcaatg
    25321 agcttaaggt cgatgatatt gttttgttaa taaatgaggt gcgtgccgat agttttaata
    25381 tatattttga caaagtgtgt attacagatg aaaatggaga tatagatgct ctaggtattc
    25441 ctgttgtttc aacttcatat aatgaacgtg ttatttcttg ggtagacgtt tcatatacag
    25501 aaaatctaac taatattgat gctaaaatta aaagtgctca agatgaattt tatcaccagt
    25561 tgtcacaagc tttattaaac aacataaatg agatttttgg tatacaagaa acaaaaaata
    25621 tgttagatca gtttgaaaat cggtatcctg atctattaaa ggaagtcttc cgacatgtga
    25681 ctatacagag aatttctgag gtattacaaa gattgcttgg agaaaatatt tctgttcgca
    25741 atttaaaact tattatggag tctttggcgc tttgggctcc cagagaaaaa gatgtcataa
    25801 cattagttga acatgtccgt gcatcacttt ctaggtatat ttgtagtaaa atagctgttt
    25861 ctggtgagat taaagttgtg atgctttccg gatatattga ggatgcaata agaaaaggga
    25921 taaggcaaac ctctggtggc tctttcttga atatggatat agaggtttcg gatgaggtaa
    25981 tggaaacttt agcacatgct ttgagagaat tgagaaatgc aaaaaaaaat ttcgttcttt
    26041 tggtatcagt agatatacgt aggtttgtta aaagacttat agataacaga tttaagagta
    26101 tactcgttat atcgtatgct gagattgatg aagcatatac cattaatgta ttaaagacta
    26161 tttagtgagg tttaaatatg agtaacatta atttagttca attagttaga gatagtcttt
    26221 tcacgattgg ttgtcctcca tcaattatta cagatttaga cagccattca gcgattacca
    26281 tttctttgga tagtatgcct gctattaata tagctcttgt caatgagcag gttatgttat
    26341 gggctaattt tgatgcacca agtgatgtaa aacttcagtc atctgcttat aacatattaa
    26401 atttaatgtt aatgaacttt agttatagca ttaatgagct ggtagagctt catcgttctg
    26461 atgagtactt acagttaaga gttgttatta aagatgacta cgttcacgat gggattgttt
    26521 ttgcagagat tctgcatgag ttttatcaga ggatggaaat cttaaatggg gtcttataat
    26581 caatgagcta tacaaaattg ctcactcaat tatcttttcc taatagaatc tcggggccaa
    26641 tcttggaaac aagtcttagc gatgtttcga ttggtgagat ttgtaacatt caggctggaa
    26701 ttgaaagtaa tgaaattgtt gcaagagctc aggttgtagg atttcatgat gaaaaaacaa
    26761 tattaagctt gattggaaat tctcgtggac tttcacggca aacgttgatt aagcccactg
    26821 cccagtttct tcatacgcaa gtcggccgtg gattattggg agcagtagtc aatcctttag
    26881 gggaggttac tgataagttt gctgttacag ataacagtga aattctttat cgacctgtag
    26941 ataatgctcc tccgctatat agtgaaaggg ctgcaattga gaagcctttt ttaacaggta
    27001 ttaaggttat tgattcttta ctcacgtgtg gtgaaggaca gcgaatgggg atttttgcgt
    27061 cagctggttg tggcaaaact tttctcatga atatgctcat tgaacatagt ggtgctgata
    27121 tatatgttat tgggttaatt ggtgagcgag gtcgagaggt tactgaaacg gttgattatt
    27181 tgaaaaactc tgagaaaaaa agcaggtgtg ttttagtata tgcaacttcg gattactctt
    27241 cggttgatcg ttgtaatgct gcatatatag ccactgctat agccgaattt tttaggactg
    27301 aaggacataa agtagcgctt tttattgatt cattaacaag gtatgccaga gcattacgtg
    27361 atgtggcctt agccgctgga gaatcacctg ccagaagagg ctatccggtt tcggtttttg
    27421 atagcttacc cagacttctt gaaaggccag gaaagttaaa ggcaggtggc tctattactg
    27481 cattttacac tgttcttttg gaggatgatg attttgctga tccattagct gaagaggtaa
    27541 gatccatttt agatggacat atatatttga gcagaaatct agcccaaaaa ggacaatttc
    27601 ctgcaattga ttccttaaaa agtataagca gggtatttac acaggttgtt gatgaaaaac
    27661 atcgtattat ggccgctgca tttcgggagc tactttctga aatagaagag ctaaggacaa
    27721 ttattgactt tggtgaatac aaaccggggg agaatgcctc tcaggataaa atctacaaca
    27781 aaatatctgt tgttgagagt tttctgaagc aagattatcg actcggtttt acttatgagc
    27841 agacaatgga gcttattggt gaaacaatta gataaggttt taaaaatcaa agataaatat
    27901 caacggtctg ttaaattaat tgaagcacac atattaacac taaaaaaaaa actctttgta
    27961 tcgagatgtg gaggcattag ataaaaggat tatatacttc ctgcaactgg agaatgactt
    28021 agaacctgta ggcgctcaga gtgttagtca gcttttcaat acaagacgta agatcgctat
    28081 agtaaaaaag catattatcc aatatcagtc tgaacgaatt cttctaaagg gaagaattga
    28141 agaaattcaa aaagatattg atgaggcaaa tgcttctaaa agaaaactgc ttcataaaga
    28201 gagtaaaata tgtaaacgga ttggcttgat taaaagaaat aactttgcca aacagttgat
    28261 acttgatgag ttatcacaag aggatatgaa gtatggcatt agataatata aacctaaatt
    28321 ttagtagtga caaacagata gaaaaatgtg agaaactatc ttcaatagat aatattgata
    28381 gtctcgtttt gaagaagaag aggaaggttg aaattcctga gtactcttta attgcatcta
    28441 actattttac tattgataag cactttgaac ataagcatga taaaggagaa atttatagtg
    28501 gcattaaaaa tgcgttcgaa cttagaaacg aacgagcgac atattctgat attccagaat
    28561 caatggccat taaagaaaat attttgatac cagatcaaga tatcaaagca agggaaaaaa
    28621 taaatatcgg cgatatgagg gggatctttt catataataa gagtggaaat gcagacaaga
    28681 acttcgaaag aagtcatact tcttctgtaa accctgataa tctgctagaa tctgataata
    28741 gaaatggtca aattggttta aaaaatcata gcttgtctat tgataagaat attgctgaca
    28801 tcatttcttt actaaatgga agtgttgcta aatcatttga gctgcctgta atgaataaaa
    28861 atactgcaga cataacccca tccatgtcat tgcaagaaaa atcaatagtt gaaaatgata
    28921 aaaatgtttt tcaaaaaaat agtgaaatga cttaccactt taaacagtgg ggggctggac
    28981 attctgttag tatttcagtg gagtctggtt cttttgttct aaaaccgtca gatcaatttg
    29041 taggaaataa acttgactta attttgaaac aagatgctga gggtaattac agatttgata
    29101 gcagtcaaca taataagggg aataaaaata atagtacagg atataatgaa cagagtgaag
    29161 aagaatgcta agaattaaac attttgacgc taacgaaaaa ctacagattt tatatgcaaa
    29221 gcaactctgc gagcgttttt cgattcagac attcaaaaat aaatttacag gcagtgaaag
    29281 tttagtcact cttacttccg tgtgtgggga ttgggtaatt cgtattgata cattatcttt
    29341 tttgaaaaaa aaatacgagg tattttcagg attttctaca caagaatctt tactgcattt
    29401 atcaaaatgt gtctttatag agtcgtcatc tgtattttcg attccagaac tgtctgataa
    29461 gattactttc cggatcacga atgaaatcca gtatgcaact actgggagtc atttatgctg
    29521 tttttcctct tctttaggta ttatttattt tgacaagatg ccggtattac gtaatcaagt
    29581 ttctcttgac tcattgcatc atcttttaga gttttgctta ggttcatcta atgtaaggct
    29641 ggctacttta aaaagaattc gcactggtga tataatcata gttcagaaac tttataattt
    29701 attattgtgt aatcaagtta ttattgggga ttatattgtg aatgataata atgaggcaaa
    29761 aattaatctg tcagaaagta atggtgagtc agaacacaca gaagtttctt tggcattatt
    29821 caattatgat gatatcaatg taaaagtgga ctttattctt ttagaaaaaa atatgacaat
    29881 caatgaacta aaaatgtatg tagaaaacga attattcaag tttcccgatg acatagttaa
    29941 acatgtaaat attaaagtaa atggttcttt ggttgggcat ggggaacttg tttctattga
    30001 ggatggttat ggtatcgaga ttagttcttg gatggtaaag gagtaatctc atgctgagtg
    30061 acatgtccct catcgctaca ctttcttttt ttacgctatt accattttta gttgctgctg
    30121 gtacctgtta tataaagttt tctattgttt ttgtaatggt acgaaatgca cttggtttgc
    30181 agcaggttcc atcaaatatg actctcaatg gtattgcatt gattatggct ttgttcgtga
    30241 tgaaacccat tattgaagct ggttatgaaa attatctaaa tggccctcaa aaatttgata
    30301 caatatctga tattgtccgt ttttctgatt ctggcttgat ggaatataag caatacttga
    30361 aaaagcatac agatcttgag ttagcacgtt ttttccaaag gtctgaagaa gaaaatgctg
    30421 atttaaagag tgcagagaat aatgattatt cattgttttc cctcttgcct gcatatgcgc
    30481 taagtgaaat caaagatgcg ttcaaaatag gtttttattt atatttgcca tttgttgttg
    30541 ttgatcttgt tatttcaagc attttacttg cacttggtat gatgatgatg agtccaatca
    30601 caatatcagt accaataaaa cttgtattat ttgttgcgct tgatgggtgg ggtatcctgt
    30661 ctaaggcctt gattgaacaa tatatcaata ttcctgctta gatgatggag gtaatgtgtc
    30721 tgatatagtt tatatgggta ataaggctct ttatttaatc cttatctttt ccttatggcc
    30781 agtaggtata gctacggtta ttggattatc tattggtttg ttacagacag tgactcaact
    30841 ccaagagcag acacttcctt ttggtataaa gcttataggt gtctcaatat ctttgctact
    30901 tctttctgga tggtatggtg aggttttatt gtctttttgt catgaaataa tgtttttaat
    30961 taagagtggg gtttgatgga catttcaagc tggttcgaaa gtattcatgt gtttttaata
    31021 ctcctgaacg gcgttttttt tagattggct ccattatttt tctttcttcc atttttaaat
    31081 aacggtataa tttctccatc tattagaata cctgtgattt ttcttgttgc atcaggatta
    31141 attacttctg gtaaggtaga cataggttct tctgtttttg aacatgttta tttccttatg
    31201 ttcaaggaaa taattgttgg cctccttctc tctttttgct tgtctcttcc cttttggata
    31261 tttcatgctg ttggtagcat tattgacaac cagcgtgggg caacgcttag tagttcaatt
    31321 gatcctgcca atggtgttga tacgtctgag ttggcaaaat ttttcaatct tttttctgca
    31381 gttgtatttc tatacagtgg tggtatggtc tttattttag aatccataca attgtcttat
    31441 aatatatgcc cgttattttc tcaatgttct ttccgtgtct caaatatctt aacatttctg
    31501 actttattgg caagtcaggc tgttatttta gccagtcctg ttatgatagt attgttacta
    31561 tcagaagtat tacttggtgt attatcgaga tttgctccgc agatgaatgc tttttccgta
    31621 tcattaacta ttaaaagttt acttgcaata tttattattt tcatctgttc ttctactatt
    31681 tacttttcta aagttcaatt tttcctcggt gaacataaat ttttcacaaa tctatttgtt
    31741 agataaaata ttatggcaaa taaaacagaa aagccgacac ctaaaaaact aaaggatgcc
    31801 gcaaaaaaag gacagtcatt taaatttaag gatttaacga ctgttgttat tattctggta
    31861 gggacattta ctataatatc attcttttcc ttaagtgatg taatgctctt atacagatat
    31921 gtaataatta atgacttcga aattaatgag ggtaaatact tttttgcagt ggttattgtc
    31981 ttttttaaga taattggctt cccacttttt ttctgtgttc tttcggctgt gttgccaaca
    32041 ttggttcaaa caaagtttgt tcttgcgact aaagctatca agattgattt ttcagtatta
    32101 aaccctgtta aagggttaaa aaaaatattt agtataaaga caataaaaga atttttcaaa
    32161 agcattctgc ttcttattat tctagcatta acaacctatt tcttttggat taatgaccga
    32221 aaaataattt tttctcaggt gttttctagt gttgatggct tatatcttat ttgggggagg
    32281 ctgtttaagg atataatatt atttttcttg gcattttcta ttcttgttat tattcttgac
    32341 tttgtgattg agttcatttt atacatgaaa gatatgatga tggataaaca ggagataaaa
    32401 agagaatata tagagcaaga gggacacttt gagacaaagt cgagaaggcg tgagttgcat
    32461 atcgagattc tttcagagca gactaaatct gatatacgta attcaaaatt agtggtaatg
    32521 aacccgactc atattgcaat tggtatttat tttaatccag aaatagcgcc tgcacctttt
    32581 atttctctca ttgaaactaa ccagtgtgcc ttggctgtca gaaaatatgc aaatgaagtt
    32641 ggtataccga ctgtgcgtga tgtgaaatta gctagaaaac tatataaaac acatacaaaa
    32701 tatagttttg ttgattttga acacttggat gaagtcctac gtcttattgt ttggcttgag
    32761 caggttgaaa acactcatta ataagtaaag gagatgtgtt atatgggagt taatttctgt
    32821 aataaaatag gtattgatca gtctgaattt gaaatagaat cttccatcat taactccatt
    32881 gctaatgagg tattgaaccc aatatctttc cttagcaata aggatataat aaatgtttta
    32941 ctcagaaaga tttcatctga atgtgacctg gttagaaaag acatttatcg ttgtgctctg
    33001 gagttggtcg ttgaaaaaac acctgatgat ttataatcat tcataataac tcttttgctg
    33061 atataaatat ttttgttgaa gatatttatg tagtcacagg atcaattgta gaaaattgtt
    33121 atgtttttgt taggaactgc actaaataat atatgataag acagcaaaaa cgactgacca
    33181 taatccttct tttattagga gttgataaga gagactattc atcctgtaat gttaaaactc
    33241 tgttatatag tattcgcgat tatgcaaagt ctgtaaatga acatgagata ctaaccgaga
    33301 gtaatcgttt attatctcat tgcatttcag atagtaacgg agctttcttt aaaagtagta
    33361 aatacgttcc ccttaagtac ttacgtaaaa ggagaatagc acgaaagata ccaaatgatt
    33421 agcactttgc tagtaagctt gctggtaaac tatattgacc gttgggtttg ttttgagact
    33481 gtaatttaaa ttgtgtaatt gcctgttttt gatatgttta ctccaacaac ggagacaggt
    33541 aaattatgga cgaaaagaag cttaaagccc t
//
LOCUS       DAJTYL010000037        31564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYL010000037 DAJTYL010000000
VERSION     DAJTYL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.037"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO572_001673"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO572_001673"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6660716.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO572_001674"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO572_001674"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6660717.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO572_001675"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO572_001675"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6660718.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO572_001676"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO572_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6660719.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO572_001677"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO572_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6660720.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO572_001678"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO572_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6660721.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO572_001679"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO572_001679"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6660722.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO572_001680"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO572_001680"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6660723.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO572_001681"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO572_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6660724.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO572_001682"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO572_001682"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6660725.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO572_001683"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO572_001683"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6660726.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO572_001684"
     CDS             8212..9075
                     /locus_tag="OO572_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6660727.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO572_001685"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO572_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6660728.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO572_001686"
     CDS             10411..11028
                     /locus_tag="OO572_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6660729.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO572_001687"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO572_001687"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6660730.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO572_001688"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO572_001688"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6660731.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO572_001689"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO572_001689"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6660732.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO572_001690"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO572_001690"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6660733.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO572_001691"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO572_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6660734.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO572_001692"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO572_001692"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6660735.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO572_001693"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO572_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6660736.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO572_001694"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO572_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6660737.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO572_001695"
     CDS             21859..23568
                     /locus_tag="OO572_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6660738.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO572_001696"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO572_001696"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6660739.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO572_001697"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO572_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO572_001698"
     tRNA            27625..27719
                     /locus_tag="OO572_001698"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO572_001699"
     CDS             27991..29202
                     /locus_tag="OO572_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6660740.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO572_001700"
     CDS             29614..30657
                     /locus_tag="OO572_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6660741.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31564
                     /locus_tag="OO572_001701"
     CDS             31172..>31564
                     /locus_tag="OO572_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HCR6660742.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGE"
BASE COUNT         7908 a         8320 c         7830 g         7506 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgag
//
LOCUS       DAJTYL010000038        30843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYL010000038 DAJTYL010000000
VERSION     DAJTYL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.038"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            177..1835
                     /locus_tag="OO572_001702"
     CDS             177..1835
                     /locus_tag="OO572_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6660743.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1832..2788)
                     /locus_tag="OO572_001703"
     CDS             complement(1832..2788)
                     /locus_tag="OO572_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6660744.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2939..4054
                     /gene="macA"
                     /locus_tag="OO572_001704"
     CDS             2939..4054
                     /gene="macA"
                     /locus_tag="OO572_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6660745.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4051..5997
                     /gene="macB"
                     /locus_tag="OO572_001705"
     CDS             4051..5997
                     /gene="macB"
                     /locus_tag="OO572_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6660746.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6070..6294)
                     /gene="cspD"
                     /locus_tag="OO572_001706"
     CDS             complement(6070..6294)
                     /gene="cspD"
                     /locus_tag="OO572_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6660747.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6617..6937
                     /gene="clpS"
                     /locus_tag="OO572_001707"
     CDS             6617..6937
                     /gene="clpS"
                     /locus_tag="OO572_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6660748.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6968..9244
                     /gene="clpA"
                     /locus_tag="OO572_001708"
     CDS             6968..9244
                     /gene="clpA"
                     /locus_tag="OO572_001708"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6660749.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9647..9734)
                     /locus_tag="OO572_001709"
     tRNA            complement(9647..9734)
                     /locus_tag="OO572_001709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9698..9700),aa:Ser,seq:gga)
     gene            complement(9988..10206)
                     /gene="infA"
                     /locus_tag="OO572_001710"
     CDS             complement(9988..10206)
                     /gene="infA"
                     /locus_tag="OO572_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6660750.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10491..11195)
                     /gene="aat"
                     /locus_tag="OO572_001711"
     CDS             complement(10491..11195)
                     /gene="aat"
                     /locus_tag="OO572_001711"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6660751.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11237..12958)
                     /gene="cydC"
                     /locus_tag="OO572_001712"
     CDS             complement(11237..12958)
                     /gene="cydC"
                     /locus_tag="OO572_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6660752.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12959..14725)
                     /gene="cydD"
                     /locus_tag="OO572_001713"
     CDS             complement(12959..14725)
                     /gene="cydD"
                     /locus_tag="OO572_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6660753.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14848..15813)
                     /gene="trxB"
                     /locus_tag="OO572_001714"
     CDS             complement(14848..15813)
                     /gene="trxB"
                     /locus_tag="OO572_001714"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6660754.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16357..16851
                     /gene="lrp"
                     /locus_tag="OO572_001715"
     CDS             16357..16851
                     /gene="lrp"
                     /locus_tag="OO572_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6660755.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16986..21014
                     /gene="ftsK"
                     /locus_tag="OO572_001716"
     CDS             16986..21014
                     /gene="ftsK"
                     /locus_tag="OO572_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6660756.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21169..21780
                     /gene="lolA"
                     /locus_tag="OO572_001717"
     CDS             21169..21780
                     /gene="lolA"
                     /locus_tag="OO572_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6660757.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21791..23134
                     /gene="rarA"
                     /locus_tag="OO572_001718"
                     /gene_synonym="mgsA"
     CDS             21791..23134
                     /gene="rarA"
                     /locus_tag="OO572_001718"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6660758.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23225..24517
                     /gene="serS"
                     /locus_tag="OO572_001719"
     CDS             23225..24517
                     /gene="serS"
                     /locus_tag="OO572_001719"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6660759.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24757..27200
                     /gene="dmsA"
                     /locus_tag="OO572_001720"
                     /pseudo
     CDS             24757..27200
                     /gene="dmsA"
                     /locus_tag="OO572_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27211..27828
                     /gene="dmsB"
                     /locus_tag="OO572_001721"
     CDS             27211..27828
                     /gene="dmsB"
                     /locus_tag="OO572_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6660760.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27830..28621
                     /locus_tag="OO572_001722"
     CDS             27830..28621
                     /locus_tag="OO572_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6660761.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28657..29283)
                     /locus_tag="OO572_001723"
     CDS             complement(28657..29283)
                     /locus_tag="OO572_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6660762.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29598..30746
                     /locus_tag="OO572_001724"
     CDS             29598..30746
                     /locus_tag="OO572_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6660763.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7589 a         7586 c         8299 g         7369 t
ORIGIN      
        1 ctaattattt gattttgttg tttttgcggg cttatcagca agagggagta taacgcgatt
       61 attcgctcat ttttcagaca attgccatgc ttaaatgtga tgtcatcacg tattagcaag
      121 gcctttcccg ttatactgcg agcgtaaagg ataagtcaca tatttctgga ggggatatga
      181 ttcttgagcg cgttgaaatt gtgggttttc gcggtatcaa ccgtttgtcg ttgatgctcg
      241 aacaaaacaa cgtcctgatt ggggagaacg cgtggggtaa atccagcttg ctggacgcct
      301 taaccctgct gctatcgcca gaatcagatc tctaccattt tgagcgcgac gatttctggt
      361 tcccgccggg agatatcaac gggcgagaac atcatctgca tattattttg accttccgcg
      421 aatcgctgcc aggccgacat cgggttcgcc gttatcggcc gctggaagcg tgctggacgc
      481 catgcaccga tggctatcac cgtatttttt atcgtctgga aggggagagt gcggaagacg
      541 gcagcgtgat gacactgcgc agttttctcg ataaagacgg acatccgatt gatgttgagg
      601 atattaacga tcaggcacgc catctggtgc gtttaatgcc ggtgctgcgc ttgcgtgatg
      661 cccgttttat gcgccgtatt cgtaacggca cggtgccaaa tgtccccaat gtggaagtca
      721 ccgcgcgcca gctcgatttc ctcgcccgtg agttgtcctc acatccgcaa aatctctctg
      781 atggacagat tcgtcaggga ctttccgcaa tggtgcagtt gcttgagcat tatttctctg
      841 agcagggggc tggacaggcg cgatatcgtt taatgcggcg gcgggccagc aatgagcaac
      901 gaagctggcg ctatctggac atcatcaacc ggatgattga ccgacctgga gggcgctcgt
      961 atcgggttat tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc acattgcgac
     1021 tggataaaga cgcgcgtccg ttattgctaa ttgaagatcc agaaacccgc ctgcatccga
     1081 ttatgctttc cgttgcctgg catctgttga atcttttgcc tttacagcgt attgctacca
     1141 ccaactctgg cgagctgctt tcgttaactc cagtggaaca tgtttgccgc ctggtgcgtg
     1201 aatcttctcg tgttgccgcc tggcgtctgg ggccaagtgg cttgagcacc gaagacagcc
     1261 gccgcatctc ttttcacatt cgttttaatc gtccgtcatc gctgtttgct cgctgctggt
     1321 tgctggtgga aggggaaacg gaaacctggg ttatcaatga actggcgcgt cagtgcggac
     1381 atcattttga tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg ctaaagccac
     1441 tggttaaatt tgcccgccga atggggattg aatggcatgt actggtcgat ggcgatgaag
     1501 cagggaagaa atatgccgct acggtacgca gcctgttgaa taacgatcgg gaagccgaac
     1561 gagaacattt aacggcgtta ccggcgctgg atatggaaca ttttatgtat cgccagggat
     1621 tttccgatgt gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg aatctacgca
     1681 aaattatctc gaaagcgatc catcgctctt ccaaacccga tcttgccatt gaagtggcaa
     1741 tggaggcagg acgtcgtggt gtggactccg taccaacgct gctgaaaaaa atgttctcac
     1801 gcgtgctgtg gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg gccatttgtg
     1861 gctgaatagc gtcgagcatc tcatagcgcc gacggtattc agcccgtttt ttactggcga
     1921 tttcggcaat ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt tcagcatcac
     1981 atacgccgcc aaccgactcc cagaacgcgt tgtaatcagc atggatcttg ccttctttat
     2041 cgcgataacg caggctgcgg taaatatgcg tttcattgct gacggcaata atctgctcta
     2101 cctgcaaacg ttgggcaaac agacaggccg cttccatcac gaggcgtttg ggaaatagcc
     2161 cgtggcaggc tttcgtcgca ttctggattt cctgatgtgg aatttcccat tttgcgcctt
     2221 gcagtccgcc aataaacatc gttcttttcc cctgatattc acacagggta aacgtgatct
     2281 ctgccagagg aataccttcg ctgttgcgga acaggattgt gctgtcacct tctttatcca
     2341 ttgagatcat catggtcagc tcaagcgtga actgctcacc gtttttgcct tccagtttag
     2401 ccagttgcag cccgggggta ttcaaatata agctgaattc ttccgccgac atacacccgc
     2461 ggagtaacgc ataatggtaa cgtaacgcct ccagcaattg cttacggcta agattcgcgg
     2521 caaggtaagg gcgatgcaga cgcacaggca gtcgcggctg gcgcgttaac aacacattga
     2581 gattaggcca gtgggaaagt tcgttcatcc actcaacgct taaacgcggc ataatcaacg
     2641 agcgcagcaa aaatttctgg cgaaaactac agcgatgcca gaatttaccc ggccgacact
     2701 gtccacgtgc cagactaaga aaaagtgaca ggctgctgag agattcagat ggcgtaaagg
     2761 tccgttcagt tagctgcgac atattcatga aatcaatggt tatacatgac gtcgatttca
     2821 ccattgcgta tcttaaccaa acatcaatag tgtgattact aacgtaaatt ttagggtttt
     2881 gttgatattt cgttgaagtt aatgacccgg attggcatat ggagtattca gaatatttat
     2941 gaaaaagcgg aaaaccgtga agaagcgtta cgttattgcg ctggtgatag tcatcgccgg
     3001 actgattacg ttatggagaa ttcttaacgc acccgtgccg acttatcaga cactgattgt
     3061 gcgccccggt gatttacagc aaagcgtgct ggcgaccgga aagctggacg cgctgcgtaa
     3121 ggttgacgtg ggcgcgcagg tcagcggtca gttgaaaacg ctgtcggtgg cgattggcga
     3181 taaagtaaaa aaagaccagc ttttaggggt cattgatcct gaacaggctg aaaaccagat
     3241 caaggaggtc gaagcaacgc tgatggagct acgcgcgcag cggcagcagg cggaagcgga
     3301 gctgaaactg gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa cgcaagcggt
     3361 ttcactgcag gatctcgata ccgccgcgac ggagatggct gtgaaacagg cgcaaattgg
     3421 caccattgac gcgcaaatca agcgcaatca ggcctctctc gatacggcta aaaccaatct
     3481 cgattacacc cgtatcgttg ccccgatggc tggggaagtg acgcaaatca ccactctgca
     3541 aggccagacg gtgattgccg cacagcaagc accgaacatt ctgacgctgg cagatatgag
     3601 cactatgctg gtaaaagcgc aggtttctga agcggatgta atccacctga agccggggca
     3661 aaaagcctgg tttacggtgc ttggcgatcc actgacgcgc tacgaggggc aaatcaagga
     3721 tgtgctaccg acgccggaaa aggtaaacga cgctattttc tattacgccc gttttgaagt
     3781 acccaacccc aatggtttgc tgcggctgga tatgaccgcg caagtgcata ttcagctcac
     3841 cgatgtgaaa aatgtgttga cgatccctct gtcggcgtta ggcgatccgg ttggcgataa
     3901 tcgttataaa gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag tgacgattgg
     3961 cgcacgtaac gataccgatg ttgagattgt caaagggctg gaagcgggcg atgaagtggt
     4021 gattggtgag gccaaaccag gagctgcaca atgacgcctt tgctcgaatt aaaggatatt
     4081 cgtcgcagct atcctgccgg tgatgagcag gttgaggtgc tgaagggcat cagcctcaat
     4141 atttatgcgg gtgagatggt cgcgattgtt ggcgcttcgg gttccggtaa atcgaccctg
     4201 atgaatattc tcggctgcct ggataaggcc accagcggca cctatcgcgt agccggtcag
     4261 gatattgcca cgctggacgc cgatgcgctg gcgcaactgc gccgcgagca tttcggcttt
     4321 attttccagc gttaccattt gcttccgcat ttaaccgtcg agcagaacgt tgaagtaccc
     4381 gccgtctatg ccggtcttga gcggaaacag cgactgcttc gtgcccagga gttgctgcaa
     4441 cggctggggc tggaagaccg tacagagtat tatccggcac agctttcggg tggtcagcaa
     4501 cagcgcgtca gcatcgcgcg ggcattgatg aacggcggtc aggtaattct tgccgatgaa
     4561 ccgaccggcg cactggacag ccattctggc gaagaggtga tggcgatcct gcatcagctg
     4621 cgcgatcgcg ggcatacggt gattatcgtc acccacgatc cgcaggtcgc tgctcaggcc
     4681 gagcgggtga tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc cattgaaaaa
     4741 gtgaatgttg ctggcgggac ggagcctgtt gtcaatacag tgtctggctg gcggcagttt
     4801 gtcagcggtt ttaacgaggc gctgacgatg gcatggcggg cgctggcagc gaataaaatg
     4861 cgtactttac tgaccatgct ggggattatt atcggtattg cgtcggtggt ttccattgtc
     4921 gtggtgggtg acgccgccaa acaaatggtg ctggcggata ttcgttctat tggtacgaat
     4981 actattgatg tctatcccgg gaaagatttt ggcgatgacg atccgcaata tcagcaggcg
     5041 ctgaagtacg acgacttaat cgccatccaa aaacaaccgt gggtcgcctc agccacacct
     5101 gccgtctcgc aaaacctgcg cctgcgttat aacaatgttg atgttgctgc cagtgccaat
     5161 ggcgtgagcg gcgattattt taatgtctat ggcatgacct tcagtgaagg aaacaccttt
     5221 aatcaggagc agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa tactcgccgc
     5281 cagcttttcc cccataaagc agatgtggtt ggcgaggtga ttctggtcgg caatatgccc
     5341 gccagagtca ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag taaagtgctg
     5401 cgtgtctggc taccttacag cacgatgtcc gggcgagtta tgggccagtc gtggcttaac
     5461 tccattactg tcagggtgaa agaaggattt gacagcgccg aggcggaaca gcaactcacg
     5521 cgtttacttt cactgcgcca cggtaagaag gatttcttta cctggaacat ggacggcgtc
     5581 ttgaaaactg ttgaaaagac cacacgtact ttacaactgt ttctgacgct ggtggcggtg
     5641 atttcgctgg tggtgggcgg tattggtgta atgaatatta tgctggtgtc agtgaccgag
     5701 cggacgcggg aaattggcat tcgcatggct gtaggtgcgc gagcaagcga tgttttgcaa
     5761 cagttcctga tcgaagccgt actggtttgc ctggtcggtg gcgcgttggg aataacactg
     5821 tcactattaa ttgctttcac cttgcagctt ttcttacccg gctgggagat tggtttttca
     5881 ccgttggcgc tgctgctggc gtttctctgc tcgacggcca ccgggatttt atttggctgg
     5941 ttacccgcac gaaatgcggc acgactggat ccagtagatg ctctggcacg ggagtaattt
     6001 ttgagataaa aatgccagcc gatcgggctg gcattttgcc tttaggatgt acacaatgag
     6061 acagaagagc tatgcgactg ccgcttctac ttcgacgggc acaataacac tggcgtgatt
     6121 gccttttggc ccctggtgga catcaaactg aacggattgt ccagctttta gcgttctgta
     6181 accatccatc tgaatggtgg aataatgagc gaaaatatct tcgccgccgc cttcagggca
     6241 gatgaaacca aaccctttgg cattgttgaa ccacttaaca gtaccctttt ccatgcttcg
     6301 acatccttcg caaatcttat acaagtaaga tggaataaac cggggtcaga gagggggctg
     6361 ttcaaaacct cgccaactct agaaatacaa tttagagaat tagggcgagc cgtcaagcat
     6421 ttgacagggg ccagggggca ggtatgaatc aaaaatttga agcagttaac gctattgaca
     6481 ggaatgtgac agatgtcgct gatgccaacg atagatgata gttatctatc atgtggagta
     6541 gattggtcag gcaaataagc tcttgtcagc ggcagggcgt tctgccgata accgtaaccg
     6601 aagatgataa ctgacaatgg gtaaaacgaa cgactggctg gactttgatc aactggcgga
     6661 agaaaaagtt cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat tagtcaatga
     6721 tgattacact ccgatggagt ttgttattga cgtgttacaa aaattctttt cttatgatgt
     6781 agaacgtgca acgcaattga tgctcgctgt tcactaccag gggaaggcca tttgcggagt
     6841 ctttaccgcc gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg cgagggagaa
     6901 tgagcatcca ttgctgtgta cgctagaaaa agcctgaatg caggcataaa aattggggga
     6961 ggtgcctatg ctcaatcaag aactggaact cagtttaaat atggctttcg ccagagcgcg
     7021 cgagcaccgt catgagttta tgaccgtcga gcacttgtta ctggcgctgc tcagtaaccc
     7081 atctgcccgg gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc gtcaggaact
     7141 ggaagccttt attgaacaaa ccacacccgt tctgcctgcc agtgaagagg agcgcgacac
     7201 acagccgacg ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg tccagtcctc
     7261 cggtcgcaat gaggttaccg gtgcaaacgt tctggtcgct atcttcagcg aacaggagtc
     7321 gcaggcggca tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg tgaactttat
     7381 ctctcatggc acgcgtaaag acgagccgac acagtcttct gatcctggca gccagccaaa
     7441 cagcgaagaa caagctggtg gggaggaacg tatggagaat ttcacgacga acctgaacca
     7501 gcttgcgcgt gtgggcggaa tcgacccact aattggtcgt gagaaagagc tggagcgtgc
     7561 tattcaggtt ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg aatctggtgt
     7621 cggtaaaacc gcgattgcag aaggtcttgc ctggcgaatt gttcagggtg atgtgccgga
     7681 agtgatggct gactgtacga tttactctct cgatatcggt tctctgttag cgggcacaaa
     7741 atatcgcggc gactttgaaa aacgttttaa agcgttgctc aagcagctgg agcaggacac
     7801 taacagcatc ctgtttattg atgagatcca caccattatc ggtgcgggtg cagcgtctgg
     7861 tggtcaggtc gatgcggcta acctaatcaa accgttgctc tccagcggta aaattcgcgt
     7921 aattggttcg acaacctatc aggagttcag caacattttc gagaaagacc gtgctctggc
     7981 gcgtcgcttc cagaaaattg atattactga accgtcgatc gaagaaactg ttcaaatcat
     8041 caatggcctg aaaccgaagt atgaagcgca ccacgacgtg cgttataccg caaaagcggt
     8101 gcgtgcggcg gtagagctgg cggtgaaata cattaacgat cgtcatctgc cggataaagc
     8161 cattgatgtt atcgacgaag caggcgctcg cgcacgtctg atgccggtaa gcaaacgcaa
     8221 gaaaaccgtt aatgtggcgg atattgagtc cgtggtggcc cgtattgcac gcattccaga
     8281 gaagagtgtt tctcagagtg accgcgatac cctgaaaaaa ctcagcgatc gcctgaaaat
     8341 gctggtcttc ggccaggata aagccattga ggcgctgact gaagccatta agatggcgcg
     8401 tgcaggttta ggtcacgaac ataaaccggt cggttcgttc ctgtttgccg gtcctaccgg
     8461 ggtcgggaaa acagaggtga cggtacagct ttcgaaagcg ttgggcattg agcttctgcg
     8521 ctttgatatg tccgagtata tggaacgcca taccgtcagc cgtctgattg gtgcgcctcc
     8581 gggatacgtt ggttttgatc agggcggttt gctgaccgat gcggtaatca agcatccaca
     8641 tgcggtgctg ctgctggacg aaatcgagaa agcgcatccg gacgtgttca atattctgtt
     8701 gcaggtgatg gacaacggta cgctgaccga taacaacgga cgcaaagcgg acttccgtaa
     8761 cgtggtgctg gtgatgacca ctaacgccgg ggtacgggaa actgagcgca aatccattgg
     8821 tcttatccac caggataaca gcaccgatgc gatggaagag atcaagaaga tctttacacc
     8881 ggaattccgt aaccgtctcg acaacattat ctggttcgat catctgtcaa ctgacgtgat
     8941 ccatcaggtg gtggataaat tcatcgtcga gttgcaggtt cagctggatc agaaaggtgt
     9001 ttctctggaa gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt acgaccgggc
     9061 aatgggcgca cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac cgctcgccaa
     9121 cgaactgctg tttggttcgc tggtggacgg cggtcaggtg acggttgcgc tggataaaga
     9181 gaaaaatgag ctgacttacg gattccagag tgcacaaaag cacaaggcgg aagcagcgca
     9241 ttaatctgat tgtcaggtag gttggtgaag tccgtaatct cgtcaggggt tacggacttt
     9301 ttatttatgg ggtggaacgg tgaaaaccct atttttggag gtgaaggtaa gttgttgata
     9361 attagtgctg ctggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg
     9421 ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag
     9481 aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca ggcagcttag
     9541 aaattcacag gtaacatact ccacccgccc accatgttca ctgccgtaca gacagataaa
     9601 atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg
     9661 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
     9721 tcggacacct caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact
     9781 atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata
     9841 ctgtaaacaa cttgtgcagt atatctacat cgagacaagt tatggactta tacttccaaa
     9901 gtacttcata catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt
     9961 ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct
    10021 caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt
    10081 tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg
    10141 gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt
    10201 ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag
    10261 ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact
    10321 ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa
    10381 cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag cggggtaatg agcgtaacca
    10441 actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat tcattcttgt
    10501 ggtgaaaaca agcaccgtgg tacccagaaa ttattcggca atcgtccgag gcgcatttga
    10561 ttgagataat taaggtaatc ccggcgggga atttcgcagg caccaagcga tgctgtgtga
    10621 tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc ctcacagaat
    10681 accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc gccacaaaat
    10741 agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg ccagacttca
    10801 atggagtggg catgaccgag ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg
    10861 atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc aaaagcgtaa
    10921 ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg gctgatatgc
    10981 agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat ggggtcgcct
    11041 ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc agggctaaga
    11101 tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg ggaagggaag
    11161 gctattgaat ggcgagaaag ctgaaccaag cgcatgaccg caaaactcca cgcaagtcgg
    11221 atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc tgtctggcaa
    11281 gcagttctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact attatttgtt
    11341 ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc tcacgcatca
    11401 tttttgcaag caattcaagg atctggcttt cggttgtggc atctaagcct tcggtaggtt
    11461 catccagcaa caccagtggc gcatcatgta acagcgtacg ggcgatagcc agacggcgca
    11521 gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga cctgcatcct
    11581 cgagcagctt ctccagacca acgcgacgca agatctccga cagagcctca tcactactgc
    11641 caggcgaggc gagtaacaga ttatcacgca gtgtggcgct aaacagatgc actcgctgag
    11701 gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg gggctatcgt
    11761 taagcaaaat ctcgccctgt tgcgggtccc atgcgcgggt cagcagttgt aacagtgttg
    11821 atttgccgca tccggttcgc ccgagaatcg ctatatgttc cccggcgttt acctgaagag
    11881 aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca tcccgtaatg
    11941 tcagcgaaac gcgatcggca acacgagttt gggtatcagg gaaggtgacc tccggttttt
    12001 gatccgttaa gtcagagata cgtacggcag aggcaatgac ttgccccaga tgctgaaatg
    12061 cacccgttac tggtgccagt gcttcaaacg cggctaacgc acaaaagaca aacagggcaa
    12121 ttaatgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac agcatcagga
    12181 tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc agttcagatt
    12241 gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg cgataacgat
    12301 cgctggcacc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc agttgttggc
    12361 gatactgtcc gcgaagatga gtcagatttt gcccggtgct ttttcccgca cgataaaaca
    12421 gcggtggcat caggaaaagc gtcagtaaca taacgctgcc cagcgtaaag gcgagggtga
    12481 aatcaaggaa acttaacccg attgtcacca ccataatcac cacaaaagcg cccaccagcg
    12541 gcgagataac gcgcaggtaa agatgatcga gcgtatcaac atccgccacc acgcggttga
    12601 gcaattcgcc ctgacgatag cgcgccagtc cggcagggga gaggggcaac aatttgctga
    12661 aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt
    12721 caaaataacg cccggcagta cgggtgattg cggcaccacg cacgcccgca gcgggtagca
    12781 tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc
    12841 cggaaagtgt caacagaccg atactggcga gcagcgtcac aattgccagc acaataccaa
    12901 gacttaacat ccatttatga cgtttataca gtgccagata gggtagcaaa gcgcgcattt
    12961 aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc acacttaatt
    13021 ccgcgtaacg tccttgctca ataatccggc catcctgcat aacccaaatg acatcccagt
    13081 cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat
    13141 tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg ggttcatcca
    13201 acagtaatag cgaacaggga tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc
    13261 ccacggaaag gcgggcagcc tggtcgccaa caggtgtatc aacgccttgt ggcaggagcg
    13321 gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcttgt tcgctggcat
    13381 caggtcgcgc cagtagtacg ttatcccgca attttgctgc cggtaattgt gggttttgcc
    13441 caacccagga gagatgttta cgccatgatt ctggtgataa atcacgtaat tctatcccgt
    13501 tgattcgtaa tgatccctga tatgagagaa aaccagaaag cgcgttcagc agtgagcttt
    13561 tacctgaacc gctgcggcca accaacactg cacgttggcc tgctggcaaa gtaaagttca
    13621 gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg gcctcaatgg
    13681 tcagcggatc ggtcaatgct aattccgcct caccgcgctg cggatgggcg agcggggttt
    13741 ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca tgataaaacg
    13801 taccgagatc gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc agaaaacctg
    13861 cagccagcgt caccccagta tcgtagtgac caaaatccag ctcgccgaga taggaaaaac
    13921 caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga atgccggagg
    13981 ataaaaacgc cagtcgtagc acttccattg tccgttggcg gaaatcttcc gaagcagaac
    14041 gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc atgccgcgca
    14101 ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgc cggttagcat
    14161 cggcagcccc cattccaacc agcgccataa acaacggaat tagcggtgct gtgcccagca
    14221 gaatgagcgc cgcagcccag ttagagggga agatcgccac cacaatcagc aacggcaccg
    14281 acactgccag cgccatttgc ggcagatagc gtgcatagta atcatgcata tcgtcaattt
    14341 gctcgagtac cagcgtcgcc cagctccccg caggtttacc ctgaatccac gctggccctg
    14401 cttgttgcag acggtcgaga acctgacggc ggatggcaaa gcggatatgc tgcccggcgt
    14461 gataacccac ccgttcgcgt aaccagacca cccatgcgcg caatacaaag gtcagaacca
    14521 gtaacgtaaa gggaagcagc agggcttcac ggggaatatt ctccataatc atatgttgca
    14581 gaattcgcgc catgaaccag gcctgggcaa tgatcaatat gccgcttaca aagcccagca
    14641 gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac cagcgggtta
    14701 actctttttg acgagattta ttcattgcac gcttagcagg tgagttatca gaattatttg
    14761 cagagcaatg ttacaacggg gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt
    14821 ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg
    14881 ttccgcatca agtgctgcca tgcagccagt accggccgaa gtaatggcct ggcgataaat
    14941 gtgatccatc acgtcgcctg cggcaaagac gccaggaatg ctggtctggg tggcattacc
    15001 atgaataccc gactgtactt tgatgtagcc gttttccagt tccagctgcc cttcgaaaat
    15061 cgcagtattc gggctgtgac cgatagcaac aaacagaccg gcaacgtcga gtgactcgat
    15121 gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acacccattt gatcgccggt
    15181 cacttcttcc agcgtacggt tggtgtgcag aatgatgttg ccgttctcca ctttatccat
    15241 cagacgctta ataaggattt tttccgcgcg gaaaccgtca cggcggtgaa tcagatgcac
    15301 ttccgaagcg atgttagaca gatacagcgc ctcttcaacc gcggtattgc cgccgccgat
    15361 gaccgcaact ttctggttgc gatagaagaa accgtcgcag gttgcacaag cagaaacccc
    15421 acggccttta aaggcttctt cagagggcag gccgagatag cgtgcagaag ctccggtggc
    15481 aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga acggacggtt
    15541 ttgcagatcc accttgttga tatgatcaaa aatgatctca gtttcaaact tggtggcatg
    15601 ttcgtgcatg cgcgccatta ataacggacc ggtcagatcg tttggatcac caggccagtt
    15661 ttccacttcc gtggtggtgg tcagttggcc gcctttttcc atgccggtaa tcagcacagg
    15721 ttgcaggttg gcgcgcgccg cgtagacagc agcggtgtat cccgccgggc ctgaacccag
    15781 gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt tggcagacaa
    15841 tgggcaggat tgtagggaat ttacagacgt aaaaaaagag tatgacgatt ttgttaacaa
    15901 tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat cacctctgct
    15961 acttaaattt cccgctttat aagccgatta aatgatgaat aaacgcccct gttaatgaat
    16021 atctggcatg ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg tgttttgcga
    16081 aaacattcga ggaagaaaaa aacagtattc ttatatgcgc ataaccatgt atgtaaatac
    16141 catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccatt cgtgatgtcg
    16201 atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc
    16261 ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag
    16321 acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct
    16381 ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt
    16441 atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt
    16501 gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct taacccccat
    16561 tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat
    16621 gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta
    16681 gtatctggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt
    16741 aagttgctgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt
    16801 gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca
    16861 ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg
    16921 gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc
    16981 ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg
    17041 gccgccgcct tctggaggca ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg
    17101 ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac
    17161 ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt
    17221 ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc
    17281 gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg
    17341 ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt
    17401 attttgcctc cggtggcgtc attggcagct tactaagcac tacgctacaa ccactgctac
    17461 acagtagcgg gggaactatt gcgctgctct gcgtttgggc agcgggcctg acgttgttca
    17521 ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta aacattctca
    17581 ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gagtatgaag
    17641 acgacgaaga gtatgaagat gaaaatcacg gcaaacagca tgaatcacgc cgtgcccgta
    17701 ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg
    17761 ggcggcaaac agacgctgcg ttgttctccg gtaagcggat ggatgatgac gaagagatta
    17821 cctacactgc acgcggtgtg gctgccgacc cggacgacgt cctattttcg ggcaatcgtg
    17881 caacgcagcc agaatatgac gaatacgatc cattattaaa cagtgcgcca attaccgaac
    17941 ctgtcgctgt agcagctgct gctaccacgg cgacacaaag ctgggctgcg ccggttgaac
    18001 ctgtgactca gacgccgcct gttgcctctg ttgatgttcc accttcgcaa cctacagtag
    18061 cctggcagcc tgtaccgggt ccacaaacgg gagagccggt tattgctcct gcaccggaag
    18121 gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac
    18181 aaccagtaca gccgcagcag ccgtattatg cacctgcagc tgaacaacct gcgcaacagc
    18241 cgtattatgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc
    18301 agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat cagcagccag
    18361 ccgctcagga gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc
    18421 ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactat tttgaagaag
    18481 tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag
    18541 aaccggttaa agaaccagaa ccgatcaaat cttcgctgaa agcaccttct gttgcagcag
    18601 tacctccagt agaagccgct gccgctgttt ctccgctggc atctggcgtg aaaaaagcga
    18661 cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg
    18721 gtggaccgcg tccgcaggtc aaagagggga ttggcccgca gttgccacga ccgaaacgta
    18781 tccgcgtgcc aactcgtcgt gaattggcgt cttacggtat taagctgccc tcacagcgtg
    18841 cggcggaaga aaaagcccgt gaagcccagc gcaatcagta tgattctggc gatcagtata
    18901 acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacac
    18961 agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca gaagatgcag
    19021 atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa caacgttatt
    19081 ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa
    19141 tgaaagcgtt gctggatgat ggtccacacg aaccgctgtt tacgccaatt gttgaacctg
    19201 tacagcagcc gcaacaaccg gttgcaccac agcagcagta tcagcagcca caacagccgg
    19261 ttgcgccaca gcagcagtat cagcagccac aacagcaggt tgcgccgcag ccacaatatc
    19321 agcagccgca acaaccggtt gcgccacagc agcaatatca gcagccgcaa caaccagttg
    19381 cgccgcagcc gcagtatcaa cagccgcaac aaccggttgc accgcagccg cagtatcagc
    19441 agccacaaca gccagttgcg ccacagccgc aggataccct gcttcatccg ctgttgatgc
    19501 gtaatggcga cagccgtccg ttgcacaaac cgacgacgcc actgccttct ctggatttgc
    19561 tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa caaatggcgc
    19621 gtctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgtcgtt aattactctc
    19681 cggggccagt tatcactcgc tttgaactta acctggctcc tggcgtaaaa gcggcgcgca
    19741 tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg
    19801 aagttattcc tggcaaaccc tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg
    19861 tttatctgcg cgaagttttg gataacgcca aattccgcga taatccgtcg ccattaaccg
    19921 tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg aaaatgccgc
    19981 acttgttggt tgcggggact accggttccg gtaaatctgt cggtgtgaac gcgatgatcc
    20041 tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc gacccgaaaa
    20101 tgctggagct ttcggtttat gaaggcattc cgcacctgtt aacggaagtc gttactgata
    20161 tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt cggtataaac
    20221 tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg
    20281 atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt atggatgccc
    20341 agcatccggt gctgaaaaaa gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc
    20401 tgatgatgac ggtaggtaaa aaagtggaag agctgatagc tcgtctggcg caaaaagccc
    20461 gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc atcggttgat gttattactg
    20521 gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt aagattgact
    20581 cacgtaccat tcttgatcag gctggcgcgg aatcactgct gggtatgggg gatatgctct
    20641 actctgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg
    20701 aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat gttgatggca
    20761 tcacctccga cagcgaaagc gaaggtggtg cgggtggttt cgatggcgct gaagaactgg
    20821 atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg
    20881 gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc gaacagatgg
    20941 aagcgcaggg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg ctggccccac
    21001 cgccgtttga ctaattaatg cattgccgga taaggcgcgg tagcgtcgca tccggcactc
    21061 tatcaactga aaattcagta ttttcttctt tcctcaagct gattattagc ctggaataga
    21121 gagtagaggg aactcccgat cgggagtgac gtaatttgag gaataatgat gaaaaaaatt
    21181 gctatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc tgatgccgca
    21241 agcgatctga aaagccgcct ggataaagtc agcagcttcc acgccagctt cacacaaaaa
    21301 gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt
    21361 ccaaacttat tcaactggca tatgacacaa cctgatgaaa gcattctggt ttctgacggt
    21421 aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacctg gctgaaagat
    21481 gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga ctggcagcag
    21541 tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag caatggcaat
    21601 ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg
    21661 gagcaggacg atcagcgcag cagttatcag ctgaaatccc agcaaaatgg ggctgtggat
    21721 gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag
    21781 aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc aacctctggc
    21841 cgcgcgtatg cggccagaaa atttagcaca gtatatcggt cagcaacatt tgctggctgc
    21901 ggggaagccg ttgccgcgcg ctatcgaagc cgggcatttg cattctatga tcctctgggg
    21961 gccaccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg cgaacgctga
    22021 tgtggaacgt atttctgccg tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg
    22081 cgcccggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg aagttcaccg
    22141 tttcaacaaa agccagcagg atgcatttct gccacatatt gaagacggca ccatcacttt
    22201 tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc
    22261 ccgtgtttat ctgttgaaat ccctgagtac agaggatatt gagcaagtac taactcaggc
    22321 gatggaagac aaaacccgtg gctatggtgg tcaggatatt gttctgccag atgatacacg
    22381 acgtgccatt gcagaactgg tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat
    22441 gatggcggat atggccgaag tctatgatag cggtaagcgg gtcctgaagc ctgaattact
    22501 gaccgaaatc gccggtgaac gtagcgcccg ctttgataac aaaggcgatc gcttttacga
    22561 tctaatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg cgctgtactg
    22621 gtatgcgcga attattaccg ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc
    22681 gattgcgtct gaagacgtcg gtaatgccga tccacgggcg atgcaggtgg caattgcggc
    22741 ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat
    22801 tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta aagccgcgct
    22861 ggccgatgct cgcgaacgcc cggattatga cgtgccggtt catttgcgta atgcgccgac
    22921 gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tatgctcatg atgaagcaaa
    22981 cgcttatgct gccggtgagg tttacttccc gccggaaata gcacaaacac gctattattt
    23041 cccgacaaac aggggccttg aaggcaagat tggtgaaaag ctcgcctggc tggctgaaca
    23101 ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta atgttgttac
    23161 tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattcgata agcacaggat
    23221 aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc
    23281 acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaagagc gtcgtaaagt
    23341 attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga aatccattgg
    23401 ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga acaaactggg
    23461 cgaagagctg gatgcagcaa aagccgagct ggatgcttta caggctgaaa ttcgcgatat
    23521 cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga
    23581 caacgttgaa gtcagccgct ggggtacccc gcgtgagttt gactttgaag ttcgtgacca
    23641 cgtgacgctg ggtgaaatgc actctggcct cgactttgca gctgcagtta agctgactgg
    23701 ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt
    23761 tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc cgtacctggt
    23821 taaccaggac acgctgtacg gtacgggtca gctgccgaaa tttgctggcg atctgttcca
    23881 tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc caacggcaga
    23941 agttccgctg actaacctgg tgcgcggtga aatcatcgat gaagatgatc tgccaattaa
    24001 gatgaccgcc cacaccccat gcttccgttc tgaagccggt tcatatggtc gtgacacccg
    24061 tggtctgatc cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg tgcgcccaga
    24121 agactcaatg gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc tgcagttgct
    24181 gggcctgccg taccgtaaaa tcatcctttg cactggcgac atgggctttg gcgcttgcaa
    24241 aacttacgac ctggaagtat ggatcccggc acagaacacc taccgtgaga tctcttcctg
    24301 ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga
    24361 caagaaaacc cgtctggttc ataccctgaa cggttctggt ctggctgttg gtcgtacgct
    24421 ggttgcagta atggaaaact atcagcaggc tgatggtcgt attgaagtac cagaagttct
    24481 gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc tgaatctaaa
    24541 aagcgccttt agggcgcttt ttttgtctcc ctttgatacc gaacaataat tactcctcac
    24601 ttacacgtaa tactactttc gagtgaaaat ctacctatct ctttgatttt caaattattc
    24661 tatgtataca agcctatata gcgagctgct atagaaataa ttacacaata cggtttgtta
    24721 ctggaatcaa tcgtgatcaa gcttgagtga gccattatga aaacgaaaat ccctgatgcg
    24781 gtattggctg ctgaggagag tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg
    24841 gcaatggcca gcagcgcatt aacattacct tttagtcgga ttgcgcacgc tgtcgatagc
    24901 gccattccaa caaaatcaga cgaaaaggtt atctggagcg cctgtacagt taactgtggt
    24961 agtcgctgcc cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacgaca
    25021 ataccggcga tgacaattac gacggcctac actaggttcg cgcctgcctg cgtgggcgtt
    25081 ccatgcgtca ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg
    25141 cgcgcggtga aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga
    25201 ccaatatgca gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca
    25261 ccggtacgct gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc
    25321 gcctgatgaa ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa
    25381 ttgcggaagg tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata
    25441 tcgaaaacag taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg
    25501 gcggtggggt gacttactat cttgaacagg cacgccagaa atctaatgct cgcatgatca
    25561 tcatcgatcc gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc
    25621 gtccaggaac agatgccgca ctggttaacg gtctggcgta cgtcatgatc actaaaaacc
    25681 tggtggatca ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg
    25741 ccagtgcgcc gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg
    25801 tggctaaaac accggaatgg gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca
    25861 aactggctcg tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc
    25921 agcgtcacgc taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg
    25981 gtaacgttgg tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt
    26041 ttgtccgtat gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga
    26101 ccgatgccat tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag
    26161 ataagctgga tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc
    26221 agcattctga aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga
    26281 ttgtggttat cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg
    26341 actgcaccgc ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt
    26401 acgtgatttt caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa
    26461 tgaccagcga actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc
    26521 aggaagagtg gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa
    26581 cgtttgaaga gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg
    26641 ttgcttataa agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca
    26701 aaattgagat ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag
    26761 gcgatgtgat cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc
    26821 tgaacaaaca gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa
    26881 cttacggcaa cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc
    26941 ttgatgccca aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg
    27001 gtgaggttca tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg
    27061 gtgaaggcgc ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg
    27121 tactgaccac tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg
    27181 ttcaggttga aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat
    27241 tccagccgtt gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg
    27301 acgccagaag tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat
    27361 aacggtgtct ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc
    27421 gaagatccgg cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt
    27481 tttgttgtgg tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg
    27541 tatggcgcac cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat
    27601 gaccgtgttg cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg
    27661 gatttcggcc ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg
    27721 ttgccgcgag ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc
    27781 ccgaccgggg ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg
    27841 atggcatgaa tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt
    27901 tatcgttctg gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt
    27961 tatcgcctgc atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct
    28021 ccatcttggt tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact
    28081 cagtaacgaa atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct
    28141 ggcaatgctg aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt
    28201 tcttggcgtc gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc
    28261 gacctggtac agcatctgga cgccgatggg ctgggcgatg cgtctgctgc cagccatttc
    28321 tgtactggca ctggtggtga gtggcgtgat gtcagtgatg cagggcacag agctggcaac
    28381 tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg cgctgatgtc
    28441 ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac agctaaaagg
    28501 ttatcagcct gcggttccgc tactatccgt ctcattcatt ctgcttctgg caggggaatt
    28561 aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg tcgcaagcta
    28621 atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
    28681 ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg tcgccagtcc
    28741 ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc atgtcatcaa
    28801 ttgcgcacca gcttgcgaca tgcgatccca cgccgaatgc cgggtaattt cattaaaggt
    28861 ccctgatgca tcagtcacga caaagacatc aaacccttct tcaatggctg aaagcgccgg
    28921 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
    28981 tttgactgct tttacaaaat cttcgttatc ccaggcgtta atattgcccg ggcgagcaat
    29041 gtaaggtgca tcggggaatt gtgctttcag ttctagaact aatgggccgt taggaccggt
    29101 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc
    29161 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
    29221 ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt
    29281 catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac gcgatgcatt
    29341 gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa
    29401 cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt aatcatctct
    29461 tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg cgatatgcgc
    29521 gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa atttgaactt
    29581 cctcacggtt ttaattcatg tccacgtata cccggcctgt catgcttttg ctgtctggcc
    29641 tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt tggctcgccc
    29701 aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt accggcaacc
    29761 ttgtcggtac attgctgaca gggtatgtca ttaaacgcat tggctttaac cgcagctatt
    29821 atctgtcctc cttcattttt gctgctggct gtgccggcct tggcctgatg attggtttct
    29881 ggagctggtt ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt tgggtggttg
    29941 ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg cttgctgcgt
    30001 atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc aaagtctcaa
    30061 ccgagctgat gagcgtattg ccgtgggtta caggtttgac attggcaggg atcttaccgc
    30121 tgttgtttac gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg tcaattacgg
    30181 caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc tcaggaatcg
    30241 ttctgggatc tctatatggc ctgatgcctc tgtacctcaa tcacaaaggg gtgagcaatg
    30301 ccagtattgg tttctggatg gcggtactgg tcagtgcggg tatcctcgga caatggccga
    30361 ttggacgtct ggcggataag tttggtcgac tgctggtttt gcgtgttcag gtctttgtcg
    30421 tcattctcgg cagtattgcg atgcttagcc aggcggcgat ggccccagcg ttattcatcc
    30481 tcggtgccgc tggctttacg ctatatccgg tggcgatggc ctgggcttgt gagaaagttg
    30541 aacatcatca actggtggcg atgaaccaag ccttactgtt gagctatacc gtgggaagtc
    30601 tgcttggccc gtcatttagc gctatgctaa tgcagaattt ctccgataat ttattgttta
    30661 tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gcttcgcaac gccggtcata
    30721 cgccgaaacc cgttgctcac gtgtaaatga attcaagcaa agtgtgaact tactgtttca
    30781 cactctgctt ttttgtttct tctatctgac ttgctttttt ccaaatttta ttcgtttaaa
    30841 aat
//
LOCUS       DAJTYL010000039        30275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYL010000039 DAJTYL010000000
VERSION     DAJTYL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.039"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..805)
                     /gene="wcaD"
                     /locus_tag="OO572_001725"
     CDS             complement(<1..805)
                     /gene="wcaD"
                     /locus_tag="OO572_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6660764.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRL"
     gene            complement(780..1997)
                     /gene="wcaC"
                     /locus_tag="OO572_001726"
     CDS             complement(780..1997)
                     /gene="wcaC"
                     /locus_tag="OO572_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6660765.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1994..2482)
                     /gene="wcaB"
                     /locus_tag="OO572_001727"
     CDS             complement(1994..2482)
                     /gene="wcaB"
                     /locus_tag="OO572_001727"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6660766.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2485..3324)
                     /gene="wcaA"
                     /locus_tag="OO572_001728"
                     /pseudo
     CDS             complement(2485..3324)
                     /gene="wcaA"
                     /locus_tag="OO572_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3417..5579)
                     /gene="wzc"
                     /locus_tag="OO572_001729"
     CDS             complement(3417..5579)
                     /gene="wzc"
                     /locus_tag="OO572_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6660767.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5582..6025)
                     /gene="wzb"
                     /locus_tag="OO572_001730"
     CDS             complement(5582..6025)
                     /gene="wzb"
                     /locus_tag="OO572_001730"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6660768.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6031..7170)
                     /gene="wza"
                     /locus_tag="OO572_001731"
     CDS             complement(6031..7170)
                     /gene="wza"
                     /locus_tag="OO572_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6660769.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7479..7628
                     /locus_tag="OO572_001732"
     CDS             7479..7628
                     /locus_tag="OO572_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660770.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7829..9412
                     /locus_tag="OO572_001733"
     CDS             7829..9412
                     /locus_tag="OO572_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6660771.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9864..11717)
                     /gene="asmA"
                     /locus_tag="OO572_001734"
     CDS             complement(9864..11717)
                     /gene="asmA"
                     /locus_tag="OO572_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6660772.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNSQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11739..12320)
                     /gene="dcd"
                     /locus_tag="OO572_001735"
     CDS             complement(11739..12320)
                     /gene="dcd"
                     /locus_tag="OO572_001735"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6660773.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12412..13053)
                     /gene="udk"
                     /locus_tag="OO572_001736"
     CDS             complement(12412..13053)
                     /gene="udk"
                     /locus_tag="OO572_001736"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6660774.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13371..13613
                     /locus_tag="OO572_001737"
                     /pseudo
     CDS             13371..13613
                     /locus_tag="OO572_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13612..14553
                     /gene="mdtA"
                     /locus_tag="OO572_001738"
                     /pseudo
     CDS             13612..14553
                     /gene="mdtA"
                     /locus_tag="OO572_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14553..17675
                     /gene="mdtB"
                     /locus_tag="OO572_001739"
                     /pseudo
     CDS             14553..17675
                     /gene="mdtB"
                     /locus_tag="OO572_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17676..20741
                     /gene="mdtC"
                     /locus_tag="OO572_001740"
     CDS             17676..20741
                     /gene="mdtC"
                     /locus_tag="OO572_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6660775.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20742..22157
                     /locus_tag="OO572_001741"
     CDS             20742..22157
                     /locus_tag="OO572_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6660776.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22154..23557
                     /gene="baeS"
                     /locus_tag="OO572_001742"
     CDS             22154..23557
                     /gene="baeS"
                     /locus_tag="OO572_001742"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6660777.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23554..24276
                     /gene="baeR"
                     /locus_tag="OO572_001743"
     CDS             23554..24276
                     /gene="baeR"
                     /locus_tag="OO572_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6660778.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24467..24799
                     /locus_tag="OO572_001744"
     CDS             24467..24799
                     /locus_tag="OO572_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6660779.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24946..26307
                     /gene="yegQ"
                     /locus_tag="OO572_001745"
     CDS             24946..26307
                     /gene="yegQ"
                     /locus_tag="OO572_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6660780.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26649..26966)
                     /locus_tag="OO572_001746"
     CDS             complement(26649..26966)
                     /locus_tag="OO572_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660781.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27372..28271
                     /gene="yegS"
                     /locus_tag="OO572_001747"
     CDS             27372..28271
                     /gene="yegS"
                     /locus_tag="OO572_001747"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6660782.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28353..29126)
                     /locus_tag="OO572_001748"
     CDS             complement(28353..29126)
                     /locus_tag="OO572_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6660783.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29232..30272)
                     /gene="gatD"
                     /locus_tag="OO572_001749"
     CDS             complement(29232..30272)
                     /gene="gatD"
                     /locus_tag="OO572_001749"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6660784.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7003 a         8041 c         7828 g         7403 t
ORIGIN      
        1 ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat acagccagag
       61 aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc
      121 actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta
      181 tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt ttgatgctga
      241 gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc
      301 gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg
      361 aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg
      421 ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt
      481 gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga
      541 caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt
      601 taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc
      661 gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt
      721 cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc agcaggtagc
      781 tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg
      841 cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt
      901 ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt
      961 tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc
     1021 gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc
     1081 tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg
     1141 ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc
     1201 atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc
     1261 actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct
     1321 tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta
     1381 ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc
     1441 tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc
     1501 ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg
     1561 gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca
     1621 tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag
     1681 tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga
     1741 tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc
     1801 gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgacg
     1861 ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc
     1921 gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta
     1981 aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc accagcgcgt
     2041 tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg
     2101 tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac
     2161 atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat
     2221 cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc
     2281 ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat
     2341 acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg
     2401 aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt
     2461 tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca gacgtttgcc
     2521 gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat tgcggatctg
     2581 gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt tgtgcttgcg
     2641 gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca tttcgccgtg
     2701 attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt actccaccac
     2761 catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga acaaacactc
     2821 tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt tatagaacag
     2881 gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg aataaacttc
     2941 tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt gctgtttatg
     3001 ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga tcccggtgat
     3061 gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc cgctgttaat
     3121 gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact gttgcagttg
     3181 ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt agtcctggcg
     3241 caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg gcatatagat
     3301 tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg gtttgcttgc
     3361 cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag gcgtcgttat
     3421 ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc gctggcgcgg
     3481 cggaagatgg agttcagaat cacccctttc accggaatac agttttgctc aaagcggctc
     3541 agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat taacgtggtt
     3601 ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc
     3661 aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg ttcgctcatt
     3721 aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa tttggcaata
     3781 gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc cgacagccca
     3841 ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc
     3901 aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca gacaaaggtt
     3961 ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc ctgcatcatc
     4021 gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc ggttggattc
     4081 cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt tttgacgcta
     4141 tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat tccgtgttct
     4201 tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag cacacccacg
     4261 atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt cttcggtttt
     4321 agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac ggtgctggct
     4381 tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg ctgaccagac
     4441 tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc cgttacgcga
     4501 ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag cgtgcggtac
     4561 gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt cagttcgttc
     4621 aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag cggcagatca
     4681 acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc gaggcggcta
     4741 cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg
     4801 cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc gcggatctgt
     4861 tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc gttctccgtt
     4921 accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt ggtgacggta
     4981 aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc ttcttttttc
     5041 agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag ggtgtagttt
     5101 ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg gcggttaaag
     5161 gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc cgccccgaaa
     5221 atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac cgttttacca
     5281 agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg tttgttggct
     5341 aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat ttgtaccagt
     5401 gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca gagggcaaat
     5461 acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac caggcgacca
     5521 atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt ttctgtcatt
     5581 cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt tcaagtaatg
     5641 tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt tcacattcgt
     5701 tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc tcgcataagc
     5761 gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac aggcggcggc
     5821 tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg acgctgatag
     5881 cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc actttcagtt
     5941 ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata ttgccgacac
     6001 agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc tggctgtatc
     6061 cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac ggttccagcg
     6121 ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga attctgtcgc
     6181 catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa tcttgccgtt
     6241 acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt tggaggtggt
     6301 catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc taaagtccat
     6361 tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat catcattacg
     6421 cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt cgccgttttg
     6481 catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca ccacgttgcg
     6541 ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa tggtcagtgg
     6601 tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat aggccttttg
     6661 tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg tcgctaagcg
     6721 cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa ccttgccgat
     6781 atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg
     6841 gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat taagaacgtc
     6901 ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca tattggggcg
     6961 cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg gataaacatt
     7021 caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt tgcccatcgt
     7081 cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg acaccaataa
     7141 tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc agagtaatta
     7201 tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt aattgaataa
     7261 aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat tgacagaata
     7321 attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt tagctaccaa
     7381 tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga tgagaaaggg
     7441 ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa tattggctcg
     7501 ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta acaattcggc
     7561 aattggctat ttttaagaat tatattaagt atcattcaat atggttttta ggagtttctt
     7621 taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat aatgttttat
     7681 tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa tatgagataa
     7741 atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg tgacatttgt
     7801 catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt ctatctgggc
     7861 cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc tggtctttat
     7921 tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg ttaccgggct
     7981 gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc tggtcaccct
     8041 gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact taatcatgct
     8101 gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac ggctggaagg
     8161 gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg tggtgacgca
     8221 aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg tcgggatggt
     8281 cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga tgttgatggc
     8341 cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc tctgcttaag
     8401 cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa
     8461 aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca atcagttggc
     8521 tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc ggactaccga
     8581 agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg ccaaaaccgc
     8641 gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac ggcggatgat
     8701 tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt cgcgccacga
     8761 tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc tggagcgaaa
     8821 tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc tcggtgttgt
     8881 tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc tgcgggtgtt
     8941 gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc tggagcagtt
     9001 ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg tggaagggat
     9061 tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga
     9121 gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg cgaatggtca
     9181 tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa aacgtgaata
     9241 tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac ctggcgaaga
     9301 agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa
     9361 ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt gatggtatcg
     9421 gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg ttgtctgtgg
     9481 taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc gtttcctgta
     9541 ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat gttacctcgc
     9601 gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttaccg
     9661 ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt tcaccggctt
     9721 gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat cttacatatt
     9781 cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc
     9841 tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac atctttgcca
     9901 ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt
     9961 agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg ccagttgcca
    10021 taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct ttccccgttc
    10081 cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc cagattcaac
    10141 atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg
    10201 acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac gttatcgaag
    10261 ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat catctgctgg
    10321 gagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc ttgccagttg
    10381 tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt
    10441 ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttctcc
    10501 agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt
    10561 gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa acccgacttg
    10621 ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc
    10681 tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc atcaatacgc
    10741 gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt
    10801 tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc
    10861 agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc gctcagcgta
    10921 ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga ctcctgccac
    10981 tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc accctgcaac
    11041 tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga agcatctacc
    11101 gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc ggagaactca
    11161 aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg gattgtcacc
    11221 tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag actggatata
    11281 tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc
    11341 ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt
    11401 agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga
    11461 cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag agacattcgc
    11521 ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat
    11581 tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc
    11641 accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat catcagcgtc
    11701 gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg
    11761 gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc
    11821 gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc
    11881 agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca gcccggatcg
    11941 atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga acgcccgtcc
    12001 agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc cagcgccagc
    12061 tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg
    12121 cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg
    12181 tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata
    12241 cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata
    12301 tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct taacgccaat
    12361 tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag cttattcaaa
    12421 gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg
    12481 cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac
    12541 ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt taacgtcacg
    12601 cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag
    12661 ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt
    12721 cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag
    12781 gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc tgtgatccat
    12841 cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc
    12901 tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa
    12961 ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc
    13021 gataatgacg cactgatgag actgatcagt catatattta gcgacctgat taacctggat
    13081 gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgtctgat
    13141 accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa ttcccggtgg
    13201 caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt gcgcaaatta
    13261 tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc
    13321 ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta ataagcaaac
    13381 aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt
    13441 tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc acccaactcg
    13501 ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca
    13561 tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc attccaggaa
    13621 ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca gttcaaagtt
    13681 gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc taacgcccgc
    13741 cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg tcaggagctg
    13801 gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc
    13861 gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt cgatggtcgc
    13921 gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac caccggaatt
    13981 gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga aagcgatatc
    14041 gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc ctgggatcgc
    14101 accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca aatcgatgcc
    14161 acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc gctgtttccc
    14221 aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt agtgatccca
    14281 acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa tagcgaaaac
    14341 aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt ggtgatccgt
    14401 gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct gaccgaaggg
    14461 gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa agccaccagc
    14521 cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg agcagcacag
    14581 gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg atggtggcga
    14641 tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg
    14701 actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat gtcatgacct
    14761 ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg aaacagatgt
    14821 cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta acattaccgc
    14881 tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac ttgttgccga
    14941 gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg ccgatcatga
    15001 cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg gtggaaaccc
    15061 gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt tccggcggtc
    15121 agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc ggcctgacca
    15181 gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa ggtagcctcg
    15241 acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc gccgaagagt
    15301 atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc gatgtcgcaa
    15361 ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa gaacaggcca
    15421 ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc gacagcattc
    15481 ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg acagtgcttt
    15541 ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa ttgatgatgg
    15601 ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg gcgaccatca
    15661 ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg gtgtttctcg
    15721 atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga ttcgtggtcg
    15781 atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc gaaaaaccgt
    15841 tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg ctgaccttct
    15901 cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg cgactgttcc
    15961 gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg tcgctgaccc
    16021 tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa cagaacagct
    16081 tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt cgtggactgg
    16141 cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg ctgctgctta
    16201 gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac aacggcatta
    16261 ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc cagcgacaac
    16321 gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc tcatttgttg
    16381 gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac ctcaaaccgt
    16441 tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg gcggtagata
    16501 aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc gatactcagg
    16561 tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg ctcagtacct
    16621 gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat gtctccagcg
    16681 actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc gccagccgtc
    16741 tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt cagcgactga
    16801 tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac aataccgaaa
    16861 ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac ggtggcgtgg
    16921 tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc atcaaccatc
    16981 tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat tcgctgggcg
    17041 atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg gatatcacca
    17101 cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact gtctggctga
    17161 ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag agctttattc
    17221 acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg ctggcattaa
    17281 tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg ctgatcggta
    17341 ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag cgcgagcaag
    17401 gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt ccgatcctga
    17461 tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc ggggtcggcg
    17521 cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc agccaggtgc
    17581 tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca ttgtggacca
    17641 aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc ctcttcattt
    17701 accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc atactgggtt
    17761 tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt atggtcagcg
    17821 cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg ccgctggagc
    17881 gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg ctcggcagca
    17941 cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg cgtgatgtgc
    18001 aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc cgcccgacct
    18061 atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca tccgatactt
    18121 attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg atttcgcaaa
    18181 tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg cgcgtcgggc
    18241 tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc actgccatca
    18301 gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac cgctggcaga
    18361 tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt attcactaca
    18421 acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca gtgcaggatg
    18481 tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc cgcaaactgc
    18541 cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca gagttgcagg
    18601 agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc accattcgcg
    18661 cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg attctggtgg
    18721 tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg gtgccggttt
    18781 cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat aatctttcgc
    18841 tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg gtgctggaaa
    18901 acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg caaggtactc
    18961 gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg ttcctgccgc
    19021 tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg acgctttctg
    19081 tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg tgcggctgga
    19141 tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc cgcatgttgg
    19201 tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat acccgtctgg
    19261 tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa accttcttcc
    19321 cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt atttcgtttc
    19381 aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    19441 ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg tttatcaccc
    19501 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtga
    19561 agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat attcgcgttg
    19621 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    19681 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    19741 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    19801 tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac gccttcggtc
    19861 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    19921 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    19981 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg
    20041 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    20101 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    20161 cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg aactcgcagg
    20221 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    20281 gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgt
    20341 tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat
    20401 taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac
    20461 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    20521 cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta ttgtcgggcg
    20581 gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt ctggtgatga
    20641 gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    20701 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca
    20761 gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    20821 ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    20881 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    20941 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    21001 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    21061 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    21121 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    21181 cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg cactggatct
    21241 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg ttaatgccga
    21301 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    21361 tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    21421 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    21481 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    21541 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    21601 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    21661 tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc tttggttatc
    21721 gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta
    21781 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    21841 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    21901 cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    21961 ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc gacagcggca
    22021 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    22081 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    22141 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
    22201 tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    22261 gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc tgttaagtga
    22321 tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca acaacgatcg
    22381 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    22441 gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa acaacaaagt
    22501 actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    22561 cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc gcaatactga
    22621 tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc tggcaacgtt
    22681 actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg taaaacgact
    22741 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    22801 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca cgctggagaa
    22861 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    22921 agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat tcacgccgga
    22981 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca
    23041 tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
    23101 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    23161 actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc gtttaatgca
    23221 gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    23281 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    23341 tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg
    23401 caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    23461 ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca ttacagtaga
    23521 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    23581 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    23641 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actggcgtat
    23701 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    23761 ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat ggtgacggca
    23821 aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga ctacatctgc
    23881 aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg ccgttgtaaa
    23941 ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    24001 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctt
    24061 aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    24121 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaatctgcgc
    24181 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    24241 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta ctttgttagc
    24301 cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca gcaaactgac
    24361 attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca gtaaacagca
    24421 cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg ctggttggtt
    24481 tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg gcaatggtga
    24541 gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc
    24601 ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa gcaacggcaa
    24661 attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc agatgtacgc
    24721 caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac
    24781 ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggcgctttac
    24841 cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc cctaaccgct
    24901 tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt taaaccggaa
    24961 ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta tggcgcagat
    25021 gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga attcaaccac
    25081 gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt ttatgtcgtg
    25141 gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct gaaaccggtg
    25201 gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat gctggtgcgt
    25261 gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt gaactgggcg
    25321 acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg cgagctgtcg
    25381 ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga gatcttcgtt
    25441 cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta tatcaacaag
    25501 cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa cgtccaggaa
    25561 gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc ggtgcaaaat
    25621 gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat cgaagaagcc
    25681 cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta catcatgaac
    25741 tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg cgtgcattcg
    25801 ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc acaggtttac
    25861 cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct gctggaaact
    25921 ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca tactcacgac
    25981 gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt tgttggtgag
    26041 tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa attctccgtt
    26101 ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct tgagcacatg
    26161 gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac tgtgtggctc
    26221 ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt ctccggggaa
    26281 accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg atggaaaatt
    26341 cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt cgctgaaaaa
    26401 cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg taatctccct
    26461 tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg ataaccgtct
    26521 tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac gtaacggtat
    26581 cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac gcttacaaaa
    26641 aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct
    26701 tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca
    26761 tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga agcttcggct
    26821 tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat
    26881 ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa aatactaatt
    26941 aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca acgacactct
    27001 ctagtgttta aatataataa tggcatgatt attataattg aataggatta taataaatgt
    27061 tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact
    27121 ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat attaggtata
    27181 tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct
    27241 cctggtggta accactgaat cctccatact tgaactgact ttttatcctc cgactttcat
    27301 cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg
    27361 cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt aaaagtactg
    27421 acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg
    27481 tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc cggaagttgg
    27541 gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt
    27601 tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta ggaaccgcca
    27661 atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa
    27721 ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta
    27781 atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag
    27841 ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac
    27901 cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg
    27961 gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac gcgttaatta
    28021 acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat
    28081 caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg
    28141 atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg agtgggcaaa
    28201 acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat
    28261 tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg ccggatgcgg
    28321 tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc
    28381 ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat aatgtcaatt
    28441 ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga atcgagaacg
    28501 gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc ttcattaaag
    28561 gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta
    28621 atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt atggcggacc
    28681 gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg
    28741 agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt
    28801 gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc
    28861 tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc
    28921 tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta
    28981 accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca
    29041 aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc
    29101 ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta
    29161 aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca gcatcacgct
    29221 ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg agcgatgtca
    29281 cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt
    29341 aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg
    29401 gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt
    29461 gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga
    29521 ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc
    29581 tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg
    29641 ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga gctaatgtcg
    29701 atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg
    29761 gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca
    29821 tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt
    29881 agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc
    29941 gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggacaa
    30001 gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca
    30061 ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa
    30121 tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt
    30181 tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc aacgcgcacg
    30241 ataccatcag tatcattcac tactgatttc ataaa
//
LOCUS       DAJTYL010000040        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYL010000040 DAJTYL010000000
VERSION     DAJTYL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.040"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO572_001750"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO572_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6660785.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO572_001751"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO572_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6660786.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO572_001752"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO572_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6660787.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO572_001753"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO572_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6660788.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO572_001754"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO572_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6660789.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO572_001755"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO572_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6660790.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO572_001756"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO572_001756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6660791.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO572_001757"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO572_001757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6660792.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO572_001758"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO572_001758"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6660793.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO572_001759"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO572_001759"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6660794.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO572_001760"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO572_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6660795.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO572_001761"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO572_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6660796.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO572_001762"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO572_001762"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6660797.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO572_001763"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO572_001763"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6660798.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO572_001764"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO572_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6660799.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO572_001765"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO572_001765"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6660800.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO572_001766"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO572_001766"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6660801.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO572_001767"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO572_001767"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6660802.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO572_001768"
     CDS             21622..22212
                     /locus_tag="OO572_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6660803.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO572_001769"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO572_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6660804.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO572_001770"
     CDS             22684..23379
                     /locus_tag="OO572_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6660805.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO572_001771"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO572_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6660806.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO572_001772"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO572_001772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6660807.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO572_001773"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO572_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6660808.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO572_001774"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO572_001774"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6660809.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO572_001775"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO572_001775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6660810.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTYL010000041        29975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYL010000041 DAJTYL010000000
VERSION     DAJTYL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.041"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            56..1552
                     /gene="ravA"
                     /locus_tag="OO572_001776"
     CDS             56..1552
                     /gene="ravA"
                     /locus_tag="OO572_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6660811.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1546..2997
                     /gene="viaA"
                     /locus_tag="OO572_001777"
     CDS             1546..2997
                     /gene="viaA"
                     /locus_tag="OO572_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6660812.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3002..3994)
                     /gene="asnA"
                     /locus_tag="OO572_001778"
     CDS             complement(3002..3994)
                     /gene="asnA"
                     /locus_tag="OO572_001778"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6660813.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4146..4604
                     /gene="asnC"
                     /locus_tag="OO572_001779"
     CDS             4146..4604
                     /gene="asnC"
                     /locus_tag="OO572_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6660814.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4694..5137
                     /gene="mioC"
                     /locus_tag="OO572_001780"
     CDS             4694..5137
                     /gene="mioC"
                     /locus_tag="OO572_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6660815.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5516..7405
                     /gene="mnmG"
                     /locus_tag="OO572_001781"
     CDS             5516..7405
                     /gene="mnmG"
                     /locus_tag="OO572_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6660816.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7469..8092
                     /gene="rsmG"
                     /locus_tag="OO572_001782"
     CDS             7469..8092
                     /gene="rsmG"
                     /locus_tag="OO572_001782"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6660817.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8709..9089
                     /gene="atpI"
                     /locus_tag="OO572_001783"
     CDS             8709..9089
                     /gene="atpI"
                     /locus_tag="OO572_001783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6660818.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9098..9913
                     /gene="atpB"
                     /locus_tag="OO572_001784"
     CDS             9098..9913
                     /gene="atpB"
                     /locus_tag="OO572_001784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6660819.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9960..10199
                     /gene="atpE"
                     /locus_tag="OO572_001785"
     CDS             9960..10199
                     /gene="atpE"
                     /locus_tag="OO572_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6660820.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10261..10731
                     /gene="atpF"
                     /locus_tag="OO572_001786"
     CDS             10261..10731
                     /gene="atpF"
                     /locus_tag="OO572_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6660821.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10746..11279
                     /gene="atpH"
                     /locus_tag="OO572_001787"
     CDS             10746..11279
                     /gene="atpH"
                     /locus_tag="OO572_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6660822.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11292..12833
                     /gene="atpA"
                     /locus_tag="OO572_001788"
     CDS             11292..12833
                     /gene="atpA"
                     /locus_tag="OO572_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6660823.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12884..13747
                     /gene="atpG"
                     /locus_tag="OO572_001789"
     CDS             12884..13747
                     /gene="atpG"
                     /locus_tag="OO572_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6660824.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13774..15156
                     /gene="atpD"
                     /locus_tag="OO572_001790"
     CDS             13774..15156
                     /gene="atpD"
                     /locus_tag="OO572_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6660825.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15177..15596
                     /gene="atpC"
                     /locus_tag="OO572_001791"
     CDS             15177..15596
                     /gene="atpC"
                     /locus_tag="OO572_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6660826.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15947..17317
                     /gene="glmU"
                     /locus_tag="OO572_001792"
     CDS             15947..17317
                     /gene="glmU"
                     /locus_tag="OO572_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6660827.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17479..19308
                     /gene="glmS"
                     /locus_tag="OO572_001793"
     CDS             17479..19308
                     /gene="glmS"
                     /locus_tag="OO572_001793"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6660828.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19470..20291
                     /locus_tag="OO572_001794"
     CDS             19470..20291
                     /locus_tag="OO572_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6660829.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20278..22386
                     /locus_tag="OO572_001795"
     CDS             20278..22386
                     /locus_tag="OO572_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6660830.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22383..24050
                     /locus_tag="OO572_001796"
     CDS             22383..24050
                     /locus_tag="OO572_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6660831.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24053..25579
                     /locus_tag="OO572_001797"
     CDS             24053..25579
                     /locus_tag="OO572_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6660832.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25580..27196
                     /locus_tag="OO572_001798"
     CDS             25580..27196
                     /locus_tag="OO572_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6660833.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27252..27404)
                     /locus_tag="OO572_001799"
                     /pseudo
     CDS             complement(27252..27404)
                     /locus_tag="OO572_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27427..27804)
                     /locus_tag="OO572_001800"
     CDS             complement(27427..27804)
                     /locus_tag="OO572_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660834.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28214..28585)
                     /locus_tag="OO572_001801"
     CDS             complement(28214..28585)
                     /locus_tag="OO572_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660835.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28646..29143)
                     /locus_tag="OO572_001802"
     CDS             complement(28646..29143)
                     /locus_tag="OO572_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660836.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29219..>29975)
                     /locus_tag="OO572_001803"
     CDS             complement(29219..>29975)
                     /locus_tag="OO572_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6660837.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
                     AYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         7716 a         7031 c         7716 g         7512 t
ORIGIN      
        1 aacgtctata ctcgcaattt acgcagaact tttgacgaaa ggacgccact tcattatggc
       61 tcaccctcat ttattagcgg aaagaatttc ccgcctgagc agttcgttgg aaaaggggct
      121 ttatgaacgt agccacgcca tccgcttgtg tttattagcg gcattaagtg gtgaaagtgt
      181 gttcctcctt ggcccgccag gtattgccaa aagtttgatc gcccggcgct taaaattcgc
      241 ctttcagaat gcccgcgcgt ttgaatatct gatgacccgc ttctccacgc cggaagaagt
      301 ttttggtccc ctttctattc aggcgctaaa agatgaaggg cgctatgaac gtttaaccag
      361 cggttacctg ccggaagcag aaatcgtctt tctggatgag atctggaaag cgggcccggc
      421 aattcttaat accttgctca ccgccattaa cgagcgccag ttccgcaacg gtgcacacgt
      481 agaaaaaatc ccgatgcgtc tactggtggc ggcctccaac gagctgccgg aagcagacag
      541 cagtctggaa gcgttatatg accgcatgct gattcgtcta tggttagata aagtgcaaga
      601 taaagcgaat ttccgctcca tgctgaccag tcaacaggat gaaaacgaca atccggttcc
      661 tgatgccttg caggtcacag atgaagaata tgaacgctgg cagaaagaga ttggtgaaat
      721 tacgctgccc gatcatgtat ttgagctgat ttttattctg cgccagcaac tggataaatt
      781 accggatgcg ccttatgtct cggatcgtcg ctggaaaaaa gcgatccgct tattgcaggc
      841 cagcgccttt tttagcggtc gcagtgctgt tgccccggtt gatctcattt tgctgaaaga
      901 ttgcctgtgg tatgacgcgc aaagcctgaa tttgatacaa caacaaattg atgtattgat
      961 gaccggtcat gcctggcaac agcaagggat gttgacccgc ctgggcgcga ttgtgcaacg
     1021 tcacctgcaa ctccagcagc aacaaagcga taaaacagcc ttaacgttaa ttcgtctggg
     1081 cggcattttc agccgtcgtc agcagtatca acttcctgtt aacgttactg cttccactct
     1141 gactctgctg ctgcaaaaac cgttaaaact gcatgatatg gaagtggttc atatctcctt
     1201 tgagcgtagc gcgctggaac agtggctgag taaaggtggt gaaattcgcg gcaaactgaa
     1261 cggtatcggc tttgcccaga aactgaatct ggaagttgat agcacccaac atcttgttgt
     1321 acgcgatgta agtttacaag gcagtacgct ggcacttccc ggttcatcgg ctgaaggtct
     1381 gccaggtgaa ataaaacaac aactggaaga gcttgaaagc gactggcgta agcaacacgc
     1441 tttattcagc gaacagcaaa aatgtctgtt tatccctggc gactggttag gtcgcattga
     1501 agacagccta caggatgtcg gtgcacagat tcgtcaggca caacaatgct aacgctggat
     1561 acgcttaatg tgatgctggc cgtcagcgaa gagggattga tcgaagagat gatcatcgca
     1621 ctgctggcct caccgcagct ggcggtcttc tttgaaaaat tcccacgact gaaggcggca
     1681 atcactgatg atgttccccg ctggcgtgag gcgctgcgca gtcggctgaa agatgcccga
     1741 gtcccgccgg aactcaccga agaggtgatg tgctatcagc aaagccagct cctctccacg
     1801 ccgcagttta ttgtgcagct accacagatc ctggacttac tgcatcgtct gaattcccca
     1861 tgggcagaac aagcccggca gttggttgat gctaacagca caataacttc agcgttacac
     1921 acgctttttc tccagcgttg gcgtttaagt ctgatcgtgc aagcaacgac gttaaatcaa
     1981 cagctattag aagaagaacg cgaacaactg ttaagtgaag ttcaggaacg catgacgctg
     2041 agcggacaac ttgaaccgat tctcgcagat aacaataccg cagctggtcg tctgtgggat
     2101 atgagcgccg gtcaacttaa acgtggcgac tatcagttta ttgtgaaata cggtgaattt
     2161 cttaacgaac agccggaact gaaacgcctg gcagagcagc tggggcgttc tcgggaagcc
     2221 aaatcaatac cgcgcaacga tgcgcagatg gaaaccttcc gcaccatggt gcgcgaaccg
     2281 gcgacggttc ctgagcaggt tgatggtctg caacaaagcg atgatatttt acgtctcctg
     2341 ccgccagaac tggcgacact agggataacg gaactggagt atgagtttta ccgtcggctg
     2401 gtggaaaaac agttgctcac ctatcgcctg cacggtgagt cgtggcgtga aaaagtgttc
     2461 gaacgtccgg tggtacataa agattacgat gaacagccgc gcgggccgtt tattgtctgt
     2521 gtggatactt ccggctcaat gggcggcttt aatgaacagt gtgcgaaagc gttctgcctg
     2581 gctttgatgc gcattgctct cgctgaaaac cggcgctgtt atattatgct attttccacc
     2641 gagatcgtcc gttatgagct ttcaggccca caaggcatcg aacaagcaat ccgtttttta
     2701 agccagcagt ttcgtggcgg caccgatctt gccagttgtt ttcgcgccat tatggaacgc
     2761 ttgcaaagca gggaatggtt tgatgccgat gcggtggtga tttctgattt tatcgctcag
     2821 cggttgcctg acgacgtgac gagtaaagtg aaagagctgc agcgggtaca tcagcatcgc
     2881 tttcatgccg tggcgatgtc ggcacacggc aaacccggca tcatgcgcat tttcgatcat
     2941 atctggcgct ttgataccgg gatgcgaagc cgcctgctca gacgctggcg gcgataaatt
     3001 attacagcag agaagggacg ctctcgcgaa cagcagctgg ccatactcca cactgaacct
     3061 ggccgatatg cggcagttgc agcagcagca tagtcaaacg agactggccg ataccgccgc
     3121 cgatggtctg cggcatttca ccgcgcagca gcgcctgatg ccattccagc tgcagacgat
     3181 cttcatcacc ggtcagcgcc agctgatgct tcagcgtgtc ggcatccacg cggatcccca
     3241 tggaagaaag ctcaaacgca tcttccagta ccgggttcca caccagaata tcgccgttca
     3301 ggcccgcatg gcccagctct gacggggtgc tccagtcatc ataatccggt gcgcgcacgt
     3361 cgtgacgatg accatcactc aacttgccgc caatcccgac gaggaatacc gcgccaagat
     3421 ctttcgcaat cgcccgctca cgccctttgg catcgagatc cggataacga gacagtaact
     3481 cctggctgtg tacgaagtga atctgatccg gcaggaacgg tgccaggcca aactcttcgt
     3541 taaccgcagc ttcggttgct ttaattcccg cccagatcgc ctctaccgtg cttttcagag
     3601 tcgagagttg acgctcaccg tcgcccatta cgcgttccca gtcccactgg tcaacataga
     3661 ccgagtgcaa cggagaaaga cggtcttcat cggggcgaag ggctttcatg tgcgtgtaca
     3721 gcccttcgcc cgcgctgaag tcgtgttgcc ctaaggtctg acgtttccac ttcgccagtg
     3781 aatgaaccac ttcgaactgg gcatcaggca gagctttcac ttttacctgc accgcttttt
     3841 cacagcccga caagttatcc tgcgtgccat cccccacacg gctaagaatc ggcgcctgga
     3901 cttcgatcag ccccagacgt tcttccagtt ggcgagaaaa gtgagatttc acgaagctaa
     3961 tttggcgttg tttggcaatg taagcggttt tcatttttta tactcctgcg tcctgttgct
     4021 tatgattaag caacaaaaac cgacacacat gcaataatca ttcaataaaa agcctaacac
     4081 acttttgatt cattaaaaaa gacggctaaa atagaatgaa tcatcaatct gcataagaaa
     4141 atcctatgga aaattatctg atcgacaatc tggaccgtgg catcctggaa gcattaatgg
     4201 gcaatgcgcg caccgcttac gccgaactgg cgaaacaatt tggcgtcagt ccggggacga
     4261 ttcacgttcg agtagagaaa atgaagcagg cggggatcat taccggggcg cgtattgatg
     4321 tcagcccgaa gcagctcggt tatgacgtag gctgctttat cggcattata ttaaagagcg
     4381 ccaaagacta cccttccgcg ctggcaaagc tggaaagcct tgatgaagtc actgaagcct
     4441 attacacaac cggccactac agcatcttta taaaagttat gtgccgttcg atcgacgctc
     4501 tccagcatgt acttatcaac aagatccaaa caattgatga aattcagtcc accgagacat
     4561 tgatcgtcct gcaaaacccg atcatgcgta ccatcaagcc ctgatcggct tttttaatcc
     4621 catacttttc cacaggtaga tcccaacgcg ttcacagcgt acaatacgcc actcttaata
     4681 aaggtggcgg tttatggcag atatcactct tatcagcggc agcaccctcg gcggtgccga
     4741 atatgtagca gaacacctgg ctgaaaagct ggaagaggtg ggttttacca ccgaaacgct
     4801 gcacggtccg ctgttggaag atttatctgc ctcggggatc tggctggtta tcagctccac
     4861 ccacggtgcc ggagatattc cggacaacct ttctcctttc tatgaagcat tgcaggaaca
     4921 gaagcccgat ctttctgcgg tccgctttgg cacaatcggt attggcagtc gtgaatatga
     4981 caccttttgt ggggctatcg ataaactcga ggcagaactc aaaaattccg gtgcaaaaca
     5041 gacaggcgaa acactgaaga tcaacattct tgatcacgac attccggaag atccggcaga
     5101 agaatggctg ggatcgtgga ttaatttact caaataagta tacagatcgt gcgatctact
     5161 gtggataact ctgtcaggaa gcttggatca accggtagct atccaaagaa caaccgttgt
     5221 tcagtttttg agttgtgtat aacccctcat tctgatccca gcttatacgg tccaggatca
     5281 ccgatcattc acagttaatg atcctttcca ggttgttgat cttaaaaatc ggatccttgt
     5341 tatccacagg gcagtgcgat cctaataaga gatcacaata gaacagatct ctaaataaat
     5401 agatcttctt tttaataccc aggatcccag gtctttctca agccgacaaa gttgagtaga
     5461 atccacggcc cgggcttcaa tccattttca taccgcgtta tgcgaggcaa tcaccatgtt
     5521 ttatccggat ccttttgacg tcatcatcat tggcgggggt catgcaggca ccgaggccgc
     5581 gatggctgcg gcgcgtatgg gtcaacagac tctgcttttg acacacaata tcgacactct
     5641 ggggcagatg agctgcaacc cggcgatcgg cggtattggg aagggacatc tggtaaaaga
     5701 agtggatgca ctcggcggtc tgatggcgaa agcgatcgat caagcgggta tccagtttag
     5761 gatactaaac gcaagcaaag gaccggcggt tcgcgctacc cgagctcagg cggatcgtgt
     5821 gcgctaccgt caggcggtac gtacggcgct ggagaaccaa ccgaacctga tgatcttcca
     5881 gcaggcggtt gaagatctta ttgtcgaaaa cgatcgcgtg gtcggtgctg ttacccaaat
     5941 gggactgaag ttccgtgcca aagctgtggt gctcaccgtt gggacgttcc tcgacggcaa
     6001 aattcatatc ggtctggata attacagcgg tggccgtgct ggcgatccgc catccattcc
     6061 gctctctcgc cgtttgcgtg aactgccgct gcgcgttggt cgtctgaaaa ccggtactcc
     6121 gccgcgtatt gatgctcgaa ccatcgactt tagcgtgctg gcgcaacagc atggcgataa
     6181 cccaatgccg gtattctcgt ttatgggcaa tgcgtccctg catccccagc aggtgccgtg
     6241 ttatatcact cataccaacg agaaaaccca tgatgtgatc cgcagtaacc tcgatcgtag
     6301 cccaatgtat gcaggggtga tcgaaggtgt cggcccacgc tactgcccgt cgatcgaaga
     6361 caaagtcatg cgcttcgcag acagaaatca gcatcagatc ttccttgaac cggaaggact
     6421 gacctctaat gaaatttatc cgaacggtat ctccaccagc ctgccgttcg atgtgcagat
     6481 gcaaatcgtc cgctctatgc aggggatgga aaacgcgaag atcgtgcgtc cgggttatgc
     6541 cattgagtat gacttcttcg atcctcgcga cctgaaaccg acgctggaga gcaagtttat
     6601 ccaggggctg ttctttgctg gtcagattaa cggcactacc ggttacgaag aagccgctgc
     6661 gcaaggtttg ctggccggtc ttaacgctgc ccgtctgtct gctgacaaag aaggttgggc
     6721 tccggcacgt tctcaggcgt atctcggcgt actggttgat gacctgtgca ctttaggaac
     6781 caaagaaccg tatcgtatgt ttacttcgcg cgcagaatat cgtctgatgc tgcgcgaaga
     6841 taatgcggat ctgcgtttga ctgaaatcgg tcgtgaactg ggcctggtgg atgacgaacg
     6901 ttgggcgcgc tttaacgaga aacttgagaa tatcgagcgt gagcgtcagc gtctgaaatc
     6961 gacctgggta accccgtcgg cggaagctgc agccgaagtg aatgctcacc tgactgcgcc
     7021 gctttcccgt gaagccagtg gtgaagatct gctgcgtcgt ccggaaatga cttatgaaaa
     7081 attaaccacg ctgacgccgt ttgcccctgc gttgacagac gaacaggcgg cggaacaggt
     7141 tgagattcag gttaaatacg aaggttatat cgcgcgccag caagatgaga tcgaaaagca
     7201 gctgcgtaac gagaacaccc tgctacccgc gacactggat taccgccaga tatccggtct
     7261 ttctaacgaa gtgatcgcca aacttaacga tcacaaaccg gcctctatcg gccaggcttc
     7321 gcgtatttct ggcgtcacgc ctgcggccat ctccattctg ctggtgtggc tgaaaaaaca
     7381 gggtatgctg cgtcgtagcg cataacgcat taaaaatgcc tggtaagcac ccgcttacca
     7441 ggcaacgcat caagaacagg taatcaccgt gctcaacaaa ctctccttac tgctgaaaga
     7501 cgcaggtatt tcgcttactg atcaccagaa aaaccagctt attgcctacg tgaatatgct
     7561 gcataaatgg aacaaagcgt acaacctgac ttcggtccgc gatcctaatg agatgctggt
     7621 acgccatatt ctcgatagca ttgtggtggc accgtatctg caaggtgaac ggtttatcga
     7681 tgtcggcacc ggaccaggac tgccaggcat tccactctct atcgtgcgtc ctgaagccca
     7741 tttcactctg ttggatagcc ttggtaaacg cgtgcgtttc cttcgtcagg tgcaacatga
     7801 gcttaaactg gagaatattg aaccagtaca gagcagggta gaagagtttc cttcagagcc
     7861 gccatttgat ggcgtaatta gccgcgcttt tgcctctctg aacgatatgg tgagctggtg
     7921 ccaccatctt cctggtgagc aaggccgttt ctacgcgctg aaagggcaaa tgccggaaga
     7981 tgaaatcgct ttgttgcccg aagaatatca ggtcgaatca gtggttaaac ttcaggttcc
     8041 agccctggat ggcgaacgtc atctggtggt gattaaagca aataaaattt aatttttatc
     8101 aaaaaaatca taaaaaattg accggttaga ctgttaacaa caaccaggtt ttctactgat
     8161 ataactggtt acatttaacg ccacgttcac tcttttgcat caacaagata acgtggcttt
     8221 ttttggtaag cagaaaataa gtcattagtg aaaatatcag tctgctaaaa atcggcgcta
     8281 agaaccatca ttggctgtta aaacattatt aaaaatgtca atgagtggtt tttgttgtgt
     8341 aaatgttatt tattaaaaca gtatctgttt ttagactgaa atatcataaa cttgtaaagg
     8401 catcatttgc caagtaaata aatatgctgt gcgcgaacat gcgcaatatg tgatctgaag
     8461 cacgctttat caccagtgtt tacgcgttat ttacagtttt tcatgatcga acaggggtag
     8521 cggaaaagtc gcaattgtat gcactggaaa aatatttaaa catttattca ccttttggct
     8581 acttattgtt tgaaatcacg ggggcgcacc gtataatttg accgcttttt gatgcttgac
     8641 tctaagcctt aaagaaagtt ttatacgaca cgcggcatac ctcgaaggga gcaggagtga
     8701 aaaacgtgat gtctgtgtcg ctcgtgagtc gaaacgttgc tcggaagctt ctgctcgttc
     8761 agttactggt ggtgatagca agtggattgc tgttcagcct caaagacccc ttctggggcg
     8821 tctctgcaat aagcgggggc ctggcagtct ttctgcctaa cgttttgttt atgatatttg
     8881 cctggcgtca ccaggcgcat acaccagcga aaggccgggt ggcttggaca ttcgcatttg
     8941 gcgaagcttt caaagttctg gcgatgttgg tgttactggt ggtggcgttg gcggttttaa
     9001 aggcggtatt cttgccgctg atcgttacgt gggttttggt gctggtggtt cagatactgg
     9061 caccggctgt aattaacaac aaagggtaaa aggcatcatg gcttcagaaa atatgacgcc
     9121 gcaggattac ataggacacc acctgaataa ccttcagctg gacctgcgta cattctcgct
     9181 ggtggatcca caaaaccccc cagccacctt ctggacaatc aatattgact ccatgttctt
     9241 ctcggtggtg ctgggtctgt tgttcctggt tttattccgt agcgtagcca aaaaggcgac
     9301 cagcggtgtg ccaggtaagt ttcagaccgc gattgagctg gtgatcggct ttgttaatgg
     9361 tagcgtgaaa gacatgtacc atggcaaaag caagctgatt gctccgctgg ccctgacgat
     9421 cttcgtctgg gtattcctga tgaatctgat ggatttactg cctatcgact tgctgccgta
     9481 cattgctgaa catgtactgg gtctgcctgc actgcgtgtg gttccgtctg cggacgtgaa
     9541 cgtaacgctg tctatggcac tgggcgtatt tatcctgatt ctgttctaca acatcaaaat
     9601 gaaaggcatc ggcggcttca cgaaagagtt gacgctgcag ccgttcaatc actgggcgtt
     9661 cattcctgtc aacttaatcc ttgaaggggt aagcctgctg tccaaaccag tttcactcgg
     9721 tttgcgactg ttcggtaaca tgtatgccgg tgagctgatt ttcattctga ttgctggtct
     9781 gttgccgtgg tggtcacagt ggatcctgaa tgtgccgtgg gccattttcc acatcctgat
     9841 cattacgctg caagccttca tcttcatggt tctgacgatc gtctatctgt cgatggcgtc
     9901 tgaagaacat taatttacca acactactac gttttaactg aaacaaactg gagactgtca
     9961 tggaaaacct gaatatggat ctgctgtaca tggctgccgc tgtgatgatg ggtctggcgg
    10021 caatcggtgc tgcgatcggt atcggcatcc tcgggggtaa attcctggaa ggcgcagcgc
    10081 gtcaacctga tctgattcct ctgctgcgta ctcagttctt tatcgttatg ggtctggtgg
    10141 atgctatccc gatgatcgct gtaggtctgg gtctgtacgt gatgttcgct gtcgcgtagt
    10201 aagcgttgct tttatttaaa gagcaatatc agaacgttaa ctaaatagag gcattgtgct
    10261 gtgaatctta acgcaacaat cctcggccag gccatcgcgt ttgtcctgtt cgttctgttc
    10321 tgcatgaagt acgtatggcc gccattaatg gcagccatcg aaaaacgtca aaaagaaatt
    10381 gctgacggcc ttgcttccgc agaacgagca cataaggacc ttgaccttgc aaaggccagc
    10441 gcgaccgacc agctgaaaaa agcgaaagcg gaagcccagg taatcatcga gcaggcgaac
    10501 aaacgccgct cgcagattct ggacgaagcg aaagctgagg cagaacagga acgtactaaa
    10561 atcgtggccc aggcgcaggc agaaattgaa gccgagcgta aacgtgcccg tgaagagctg
    10621 cgtaagcaag ttgctatcct ggctgttgct ggcgccgaga agatcatcga acgttccgtg
    10681 gatgaagctg ctaacagcga catcgtggat aaacttgtcg ctgaactgta aggagggagg
    10741 ggctgatgtc tgaatttatt acggtagctc gcccctacgc caaagcagct tttgactttg
    10801 ccgtcgaaca ccaaagtgta gaacgctggc aggacatgct gacgtttgcc gccgaggtaa
    10861 ccaaaaacga acaaatggca gagcttctct ctggcgcgct tgcgccagaa acgctcgccg
    10921 agtcgtttat cgcagtttgt ggtgagcaac tggacgaaaa cggtcagaac ctgattcggg
    10981 taatggctga aaatggtcgt cttaacgcgc tcccggatgt tctggagcag tttattcacc
    11041 ttcgtgccgt gagtgaggct accgctgagg tagacgtcat ttccgctgcc gcactgagtg
    11101 aacaacagct cgcgaaaatt tctgctgcga tggaaaaacg tctgtcacgc aaagttaagc
    11161 tgaattgcaa aatcgataag tctgtaatgg ctggcgttat catccgatcg ggtgatatgg
    11221 tcattgatgg cagcgtacgc ggtcgtcttg agcgccttgc agacgtcttg cagtcttaag
    11281 gggactggag catgcaactg aattccaccg aaatcagcga actgatcaag cagcgcattg
    11341 ctcagttcaa tgttgtgagt gaagctcaca acgaaggtac tattgtttct gtaagtgacg
    11401 gtgttatccg cattcacggc ctggccgatt gtatgcaggg tgaaatgatc tccctgccgg
    11461 gtaaccgtta cgctatcgca ctgaacctcg agcgcgactc tgtaggtgcg gttgttatgg
    11521 gtccgtacgc tgaccttgcc gaaggcatga aagttaagtg tactggccgt atcctggaag
    11581 ttccggttgg ccgtggcctg ctgggccgtg tggttaacac tctgggtgca ccaatcgacg
    11641 gtaaaggtcc gctggatcac gacggcttct ctgctgtaga agcaatcgct ccgggcgtta
    11701 tcgaacgtca gtccgtagat cagccggtac agaccggtta taaagccgtt gactccatga
    11761 tcccaatcgg tcgtggtcag cgtgaattga tcatcggtga ccgtcagaca ggtaaaaccg
    11821 cactggctat cgatgccatc atcaaccagc gcgattccgg tatcaaatgt atctatgtcg
    11881 ctatcggcca gaaagcgtcc accatttcta acgtggtacg taaactggaa gagcacggcg
    11941 cactggctaa caccatcgtt gtggtagcaa ccgcgtctga atccgctgca ctgcaatacc
    12001 tggcaccgta tgccggttgc gcaatgggcg aatacttccg tgaccgcggt gaagatgcgc
    12061 tgatcattta cgatgacctg tctaaacagg ctgttgctta ccgtcagatc tccctgctgc
    12121 tccgtcgtcc gccaggacgt gaagcattcc cgggcgacgt tttctacctc cactctcgtc
    12181 tgctggagcg tgctgcacgt gttaacgccg aatacgttga agccttcacc aaaggtgaag
    12241 tgaaagggaa aaccggttct ctgaccgcac tgccgattat cgaaactcag gcgggtgacg
    12301 tttctgcgtt cgttccgacc aacgtaatct ccattaccga tggtcagatc ttcctggaaa
    12361 ccaacctgtt caacgccggt attcgtcctg cggttaaccc gggtatttcc gtatcccgtg
    12421 ttggtggtgc agcacagacc aagatcatga aaaaactgtc cggtggtatc cgtaccgctc
    12481 tggcacagta tcgtgaactg gcagcgttct ctcagtttgc atccgacctt gacgatgcaa
    12541 cacgtaagca gcttgaccac ggtcagaaag tgaccgaact gctgaaacag aaacagtatg
    12601 cgccgatgtc cgttgcgcag cagtctctgg ttctgttcgc agcagaacgt ggttacctgg
    12661 cggatgttga actgtcgaaa attggcagct tcgaagccgc tctgctggct tacgtcgacc
    12721 gtgatcacgc tccgttgatg caagagatca accagaccgg tggctacaac gacgaaatcg
    12781 aaggcaagct gaaaggcatc ctcgattcct tcaaagcaac ccaatcctgg taacgtctgg
    12841 aggcttgcct tagggcaggc cgcaaggcat tgaggagaag ctcatggccg gcgcaaaaga
    12901 gatacgtagt aagatcgcaa gcgtccagaa cacgcaaaag atcactaaag cgatggagat
    12961 ggtcgccgct tccaaaatgc gtaaatcgca ggatcgcatg gcggccagcc gtccttatgc
    13021 agaaaccatg cgcaaagtga ttggtcacct tgcacacggt aatctggaat ataagcaccc
    13081 ttacctggaa gaccgcgacg ttaaacgcgt gggctacctg gtggtgtcga ccgaccgtgg
    13141 tttgtgcggt ggtttgaaca ttaacctgtt caaaaaactg ctggcggaaa tgaagacctg
    13201 gaccgacaaa ggcgttcaat gcgacctcgc aatgatcggc tcgaaaggcg tgtcgttctt
    13261 caactccgtg ggcggcaatg ttgttgccca ggtcaccggc atgggggata acccttccct
    13321 gtccgaactg atcggtccgg taaaagtgat gttgcaggcc tacgacgaag gccgtctgga
    13381 caagctttac attgtcagca acaaatttat taacaccatg tctcaggttc cgaccatcag
    13441 ccagctgctg ccgttaccgg catcagatga tgatgatctg aaacataaat cctgggatta
    13501 cctgtacgaa cccgatccga aggcgttgct ggataccctg ctgcgtcgtt atgtcgaatc
    13561 tcaggtttat cagggcgtgg ttgaaaacct ggccagcgag caggccgccc gtatggtggc
    13621 gatgaaagcc gcgaccgaca atggcggcag cctgattaaa gagctgcagt tggtatacaa
    13681 caaagctcgt caggccagca ttactcagga actcaccgag atcgtctcgg gggccgccgc
    13741 ggtttaaaca ggttatttcg tagaggattt aagatggcta ctggaaagat tgtccaggta
    13801 atcggcgccg tagttgacgt cgaattccct caggatgccg taccgcgcgt gtacgatgct
    13861 cttgaggtgc aaaatggtaa tgagcgtctg gtgctggaag ttcagcagca gctcggcggc
    13921 ggtatcgtgc gtaccatcgc aatgggttcc tccgacggtc tgcgtcgcgg tctggatgta
    13981 aaagacctcg aacacccgat cgaagtcccg gtaggtaaag cgactctggg ccgtatcatg
    14041 aacgtactgg gtgaaccggt cgacatgaaa ggcgagatcg gtgaagaaga gcgttgggcg
    14101 attcaccgcg cagcaccttc ctacgaagag ctgtcaaact ctcaggaact gctggaaacc
    14161 ggtatcaaag ttatcgacct gatgtgtccg ttcgctaagg gcggtaaagt tggtctgttc
    14221 ggtggtgcgg gtgtaggtaa aaccgtaaac atgatggagc tcattcgtaa catcgcgatc
    14281 gagcactccg gttactctgt gtttgcgggc gtaggtgaac gtactcgtga gggtaacgac
    14341 ttctaccacg aaatgaccga ctccaacgtt atcgacaaag tatccctggt gtatggccag
    14401 atgaacgagc cgccgggaaa ccgtctgcgc gttgctctga ccggtctgac catggctgag
    14461 aaattccgtg acgaaggtcg tgacgttctg ctgttcgttg acaacatcta tcgttacacc
    14521 ctggccggta cggaagtatc cgcactgctg ggccgtatgc cttcagcggt aggttatcag
    14581 ccgaccctgg cggaagagat gggcgttctg caggaacgta tcacctccac caaaactggt
    14641 tctatcacct ccgtacaggc ggtatacgta cctgcggatg acttgactga cccgtctccg
    14701 gcaaccacct ttgcgcacct tgacgcaacc gtggtactga gccgtcagat cgcgtctctg
    14761 ggtatctacc cggccgttga cccgctggac tccaccagcc gtcagctgga cccgctggtg
    14821 gttggtcagg aacactacga caccgcgcgt ggcgttcagt ccatcctgca acgttatcag
    14881 gaactgaaag acatcatcgc catcctgggt atggatgaac tgtctgaaga agacaaactg
    14941 gtggtagcgc gtgctcgtaa gatccagcgc ttcctgtccc agccgttctt cgtggcagaa
    15001 gtattcaccg gttctccggg taaatacgtc tccctgaaag acaccatccg tggctttaaa
    15061 ggcatcatgg aaggcgaata cgatcacctg ccggagcagg cgttctacat ggtcggttcc
    15121 atcgaagaag ctgtggaaaa agccaaaaaa ctttaacgcc ttaatcggag ggtgatatgg
    15181 caatgactta ccacctggac gtcgtcagcg cagagcaaca aatgttctct ggtctggtcg
    15241 agaaaatcca ggtaacgggt agcgaaggtg aactggggat ctaccctggc cacgcaccgc
    15301 tgctcaccgc cattaagcct ggtatgattc gcatcgtgaa acagcacggt cacgaagagt
    15361 ttatctatct gtctggcggc attcttgaag tgcagcctgg caacgtgacc gttctggccg
    15421 acaccgcaat tcgcggccag gatctcgacg aagcgcgagc catggaagcg aaacgtaagg
    15481 ctgaagagca cattagcagc tctcacggcg acgtagatta cgctcaggcg tctgcggaac
    15541 tggccaaagc gatcgcgcag ctgcgcgtta tcgagttgac caaaaaagcg atgtaacacc
    15601 ggcttgaaaa gcacaaaagc cagtctggaa acaggctggc tttttttacg cgttgacccg
    15661 tcctgaagag cgttcacata gatcctgctg atataaaaca cccctgtttt cccgtttatt
    15721 cattgatcga aataagagca aaaacatcca cctgacgctt aaatgaaggt actgccttaa
    15781 ttttctgcag ataaaaggcg tgacgatggt cgaaaatggc actttcgtca gcggggataa
    15841 tccgttattg aacaatttat cctctgtcca tttcacgatg aaaaaaatgt agttttttca
    15901 aggtgaagcg gtttaaattc gttctcaaat tacagtcagg acgcgtatgt tgaataatgc
    15961 tatgagcgta gtgatccttg ccgcaggcaa aggcacgcgc atgtattccg atcttccgaa
    16021 agtgctgcat acccttgccg ggaaagcgat ggttcagcat gtcattgatg ctgcgaatga
    16081 attaggcgca gcgcacgttc acctggtgta cggtcacggc ggcgatctgc ttaaacaggc
    16141 gctgaaagac gacaacctta actgggtgct tcaggcagag cagctgggta cgggtcatgc
    16201 aatgcagcag gccgcacctt tctttgccga tgatgaagac attttaatgc tctacggcga
    16261 cgtgccgctg atctctgtcg aaacactcca gcgcctgcgt gatgctaaac cccagggtgg
    16321 cattggtctg ctgacggtga aactggatga tccgaccggt tatggacgta tcacccgtga
    16381 aaatggcaaa gttaccggca ttgttgagca caaagatgcc accgacgagc agcgtcagat
    16441 tcaggagatc aacaccggca ttctgattgc caacggcgca gatatgaaac gctggctggc
    16501 gaagctgacc aacaataatg ctcagggcga atactacatc accgacatta ttgcgctggc
    16561 gtatcaggaa gggcgtgaaa tcgtcgccgt tcatccgcaa cgtttaagcg aagtagaagg
    16621 tgtgaataac cgcctgcaac tctcccgtct ggagcgcgtt taccagtccg aacaggctga
    16681 aaaactgctg ttagcaggcg ttatgctgcg cgatccggcg cgttttgatc tgcgcggtac
    16741 gcttactcac gggcgcgatg ttgaaattga tactaacgtt atcatcgagg gcaacgtgac
    16801 tctcggtcat cgcgtgaaaa tcggcaccgg ttgcgtgatt aaaaacagcg tgattggcga
    16861 tgattgcgaa atcagtccgt ataccgttgt ggaagacgcg aatctggcag cggcctgtac
    16921 tattggcccg tttgcccgtc tgcgtcctgg tgctgagttg ctggaaggtg cacacgttgg
    16981 taacttcgtt gagatgaaaa aagcgcgtct gggtaaaggc acgaaagccg ggcatctgac
    17041 ttacctgggc gatgcggaaa ttggcgataa cgttaacatc ggcgcgggaa ccattacctg
    17101 caactacgat ggtgcgaata aatttaagac cattatcggc gacgatgtgt ttgtcggttc
    17161 cgacactcag ctggtggccc cggtaacagt aggcaaaggc gcgaccattg ctgcgggtac
    17221 aactgtgacg cgtaatgtcg gcgaaaatgc attagctatc agccgtgtgc cgcagactca
    17281 gaaagaaggc tggcgtcgtc cggtaaagaa aaagtaattc tggccgggta atccggtcat
    17341 ctgggatgag gagataacat aatctccctc ccacaagcag taactataaa aataacccca
    17401 ctctctacaa ggctcggggc gcccgaaaaa acgggcatac aggttgaccg acaacgatat
    17461 aaatcggaat caaaaactat gtgtggaatt gttggcgcga tcgcgcaacg tgatgtagca
    17521 gaaatccttc ttgaaggttt acgtcgtctg gaataccgcg gatatgactc tgccggtctg
    17581 gccgttgttg atgcggaagg tcatatgact cgcctgcgtc gcctcggtaa agtccagatg
    17641 ctggctcagg cagcggaaga acatcctctg catggcggca ccggtattgc tcacactcgc
    17701 tgggcgaccc acggtgaacc ttcagaagct aacgcgcatc cgcatgtttc tgaacacatt
    17761 gtggtggtgc ataatggcat catcgaaaac catgaaccgc tgcgtgaaga gttaaaagcg
    17821 cgtggctata ccttcgtttc tgaaaccgac accgaagtga ttgcccatct ggtgaactgg
    17881 gagctgaaac aaggcgggac tctgcgtgag gctgttctgc gtgctatccc gcagctgcgc
    17941 ggtgcgtacg gtacagtgat catggactcc cgtcacccgg ataccctgct agcggcacgt
    18001 tctggtagtc cgctggtgat tggcctgggt atgggcgaaa actttatcgc ttctgaccag
    18061 ctggcgctgt tgccggtgac ccgtcgcttt atcttccttg aagagggcga tattgcggaa
    18121 atcactcgcc gttcggtaaa catcttcgat aaaactggcg cggaagtaaa acgtcaggat
    18181 atcgaatcca atctgcaata tgacgcgggc gataaaggta tttaccgtca ctacatgcag
    18241 aaagagatct acgaacagcc gaacgcgata aaaaacaccc ttaccgggcg catcagccac
    18301 ggtcaggttg atttaagcga gctgggaccg aacgccgacg aactgctgtc gaaggttgag
    18361 catattcaga tcctcgcctg tggtacttct tataactccg gtatggtttc ccgctactgg
    18421 tttgaatcgc tggcaggtat tccgtgcgac gtcgaaatcg cctctgaatt ccgctatcgt
    18481 aaatctgccg tgcgtcgtaa cagcctgatg atcaccttgt cacagtctgg cgaaaccgcg
    18541 gataccctgg cgggcctgcg tctgtcgaaa gagctgggtt accttggttc gctggcaatc
    18601 tgtaacgttc cgggttcttc tctggtgcgc gaatccgatc tggcgctgat gaccaacgcg
    18661 ggtacggaaa tcggcgtggc gtccactaaa gcattcacca ctcagttaac tgtgctgttg
    18721 atgctggtgg cgaagctgtc tcgcctgaaa ggtctggatg cctccattga acatgacatt
    18781 gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca ggataaacgc
    18841 attgaagctc tggcagaaga tttctctgac aaacatcacg cgctgttcct gggccgtggc
    18901 gatcagtacc caatcgcgct ggaaggcgca ttgaagctga aagagatctc ttacattcac
    18961 gctgaagcct acgctgcagg cgagctgaaa cacggcccgc tggcgctgat tgacgcggat
    19021 atgccggtta tcgtcgttgc accgaacaac gaactgttgg aaaaactgaa atccaacatt
    19081 gaagaagttc gcgcgcgtgg cggtcaattg tatgtcttcg ccgatcagga tgcgggtttt
    19141 gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt gattgcaccg
    19201 atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat caaaggcacc
    19261 gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaatc actatcatag
    19321 ataaaaaagg gctgctgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa
    19381 ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat
    19441 gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag
    19501 tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc
    19561 catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt tattctcata
    19621 agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt ctcagtcttg
    19681 agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct agtgatacca
    19741 ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta gatcaggtta
    19801 tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt gctattcaag
    19861 tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa ctagagcgtc
    19921 gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa ataaatcccg
    19981 tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt tctgcggagc
    20041 ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat gaaaacatta
    20101 tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa acattgagtg
    20161 agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag tctttcaggg
    20221 caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag ttgcgctatg
    20281 tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt ggctatagag
    20341 gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca agctagggct
    20401 gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga tgacccttat
    20461 gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa gcgcgaggag
    20521 gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc taaagtcaga
    20581 agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac agtttataag
    20641 ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc tgactacaaa
    20701 aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat tggccgagcc
    20761 agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg cctttttagg
    20821 ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt tgcctataga
    20881 cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga ttacccaaca
    20941 ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg cttaaagtct
    21001 agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac agccacttct
    21061 caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga tatttattta
    21121 gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat tgtgattgat
    21181 gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc ttatgtggtg
    21241 gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc ccagcatgat
    21301 attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt gctagcggac
    21361 cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa tgtgcgagtg
    21421 gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac ttttagaaca
    21481 ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg gatcaaaagc
    21541 catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt cacacaaatt
    21601 attttgcgta cgatcttatt cagaaataac catctggtga tggataaata cgatcgagat
    21661 gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg gggtatgcag
    21721 catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc gttactgcct
    21781 cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta ttactcgggg
    21841 tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag acctcaacat
    21901 ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc gcaagttggc
    21961 agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa aggtctctca
    22021 ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa tgcgaagcag
    22081 gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat tcagaaagcg
    22141 aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca gattaaaact
    22201 aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt gaagccaagc
    22261 tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga tgatcaagaa
    22321 gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga aaaggatgag
    22381 tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag gcttatcgtg
    22441 ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt gctgcatcac
    22501 tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt atcagagctc
    22561 ataccatttg tcgtattcca gatgactatt ttcagccatt aggtacgcat ttgctactaa
    22621 gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg cagaaatcct aaaacaggag
    22681 atttacaaaa gcatttacaa aatggttatg agcgtgttca aacgggagag ttggagacat
    22741 ttcgctttga ggaggcacga tctacggcac aaagcttatt gttaattggt tgttctggta
    22801 gtgggaagac gacctctctt catcgtattc tagccacgta tcctcaggtg atttaccatc
    22861 gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga ctgctcgcat aatggttcgc
    22921 taaaagaaat ctgcttgaat tttttcagag cgttggatcg agccttgggc tcgaactatg
    22981 agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat gttggctttg atgtcgcaaa
    23041 tagccaatgc acatgcttta gggttgttgg ttattgatga aattcagcat ttaagccgct
    23101 ctcgttcggg tggatctcaa gagatgctga acttttttgt gacgatggtg aatattattg
    23161 gcgtaccagt gatgttgatt ggtaccccta aagcacgaga gatttttgag gctgatttgc
    23221 ggtctgcacg tagaggggca gggtttggag ctatattctg ggatcctata caacaaacgc
    23281 aacgtggaaa gcccaatcaa gagtggatcg cttttacgga taatctttgg caattacagc
    23341 ttttacaacg caaagatgcg ctgttatcgg atgaggtccg tgatgtgtgg tatgagctaa
    23401 gccaaggagt gatggacatt gtagtaaaac tttttgtact cgctcagctc cgtgcgctag
    23461 ctttaggcaa tgagcgtatt accgctggtt tattgcggca agtgtatcaa gatgagttaa
    23521 agcctgtgca ccccatgcta gaggcattac gctcgggtat cccagaacgc attgctcgtt
    23581 attctgatct agtcgttccc gagattgata aacggttaat ccaacttcag ctagatatcg
    23641 cagcgataca agaacaaaca ccagaagaaa aagcccttca agagttagat accgaagatc
    23701 agcgtcattt atatctgatg ctgaaagagg attacgattc aagcctgtta attcccacta
    23761 ttaaaaaagc gtttagccag aatccaacga tgacaagaca aaagttactg cctcttgttt
    23821 tgcagtggtt gatggaaggc gaaacggtag tgtcagaact agaaaagccc tccaagagta
    23881 aaaaggtttc ggctataaag gtagtcaagc ccagcgactg ggatagcttg cctgatacgg
    23941 atttacgtta tatctattca caacgccaac ctgaaaaaac catgcatgaa cggttaaaag
    24001 ggaaaggggt aatagtggat atggcgagct tatttaaaca agcaggttag ccatgagaaa
    24061 ctttcctgtt ccgtactcga atgagctgat ttatagcact attgcacggg caggcgttta
    24121 tcaagggatt gttagtccta agcagctgtt ggatgaggtg tatggcaacc gcaaggtggt
    24181 cgctacctta ggtctgccct cgcatttagg tgtgatagca agacatctac atcaaacagg
    24241 acgttacgct gttcagcagc ttatttatga gcatacctta ttccctttat atgctccgtt
    24301 tgtaggcaag gagcgccgag acgaagctat tcggttaatg gagtaccaag cgcaaggtgc
    24361 ggtgcattta atgctaggag tcgctgcttc tagagttaag agcgataacc gctttagata
    24421 ctgccctgat tgcgttgctc ttcagctaaa taggtatggg gaagcctttt ggcaacgaga
    24481 ttggtatttg cccgctttgc catattgtcc aaaacacggt gctttagtct tctttgatag
    24541 agctgtagat gatcaccgac atcaattttg ggctttgggt catactgagc tgctttcaga
    24601 ctaccccaaa gactccctat ctcaattaac agcactagct gcttatatag cccctctgtt
    24661 agatgctcca cgagcgcaag agctttcccc aagccttgag cagtggacgc tgttttatca
    24721 gcgcttagcg caggatctag ggctaaccaa aagcaagcac attcgtcatg acttggtggc
    24781 ggagagagtg aggcagactt ttagtgatga ggcactagag aaactggatt taaagttggc
    24841 agagaacaag gacacgtgtt ggctgaaaag tatattccgt aagcatagaa aagcctttag
    24901 ttatttacag catagtattg tgtggcaagc cttattgcca aaactaacgg ttatagaagc
    24961 gctacagcag gcaagtgctc ttactgagca ctctataacg acaagacctg ttagccagtc
    25021 tgtgcaacct aactctgaag atttatctgt taagcataaa gactggcagc aactagtgca
    25081 taaataccaa ggaattaagg cggcaagaca gtctttagag ggtggggtgc tatacgcttg
    25141 gctttaccga catgacaggg attggctagt tcactggaat caacagcatc aacaagagcg
    25201 tctggcaccc gcccctagag ttgattggaa ccaaagagat cgaattgctg tacgacaact
    25261 attaagaatc ataaagcgtc tagatagtag ccttgatcac ccaagagcga catcgagctg
    25321 gctgttaaag caaactccta acggaacctc tcttgcaaaa aatctacaga aactgccttt
    25381 ggtagcgctt tgcttaaagc gttactcaga gagtgtggaa gattatcaaa ttagacggat
    25441 tagccaagct tttattaagc ttaaacagga agatgttgag cttaggcgct ggcgattatt
    25501 aagaagtgca acgttatcta aagagcggat aactgaggaa gcacaaagat tcttggaaat
    25561 ggtttatggg gaagagtgag tggttaggct agctacattt aatgacaatg tgcaggttgt
    25621 acatattggt catttattcc gtaactcggg tcataaggag tggcgtattt ttgtttggtt
    25681 taatccaatg caagaacgga aatggactcg atttactcat ttgcctttat taagtcgagc
    25741 taaggtggtt aacagtacaa caaagcaaat aaataaggcg gatcgtgtga ttgagtttga
    25801 agcatcggat cttcaacgag ccaaaataat cgattttcct aatctctcgt cctttgcttc
    25861 cgtacgcaac aaggatggag cgcagagttc atttatttac gaagctgaaa caccatatag
    25921 caagactcgt tatcacatcc cacagttaga gctagctcgg tcattatttt taattaactc
    25981 ctatttctgt cgaagctgtt tgagcagtac cgctttacag caagagttcg acgttcagta
    26041 tgaggttgag cgagatcatt tagagataag gatcttaccc agttcatcgt ttcctaaagg
    26101 ggcgttagag ctgtcggccg tagtgcagct tttggtttgg ttgttttcgg atcaagatgt
    26161 tatggattcg tatgaaagta tttttaggca ctatcaacaa aatagagaaa ttaagaacgg
    26221 cgttgaaagc tggtgcttta gctttgaccc tccgcccatg cagggttgga aattacatgt
    26281 aaaaggacgt tcttctaacg aggataagga ttatttagtt gaggaaatag taggtttaga
    26341 aatcaacgct atgcttccta gcacaacagc tattagccat gcctcttttc aggaaaagga
    26401 ggcaggtgat ggtagtacgc agcacatagc ggtttcaaca gagtcagttg ttgatgatga
    26461 gcatctacag ttggacgatg aggaaacagc caatatagac acagacacac gagtcataga
    26521 ggctgagccg acatggataa gttttagtag acctagtcga attgaaaaat ctcgcagggc
    26581 aagaaaaagt agccaaacta ttttagaaaa agaagaagca acaacaagtg aaaatagtaa
    26641 tttggttagt actgatgagc cacacttagg tggtgtccta gcagcggcag atgtgggtgg
    26701 gaagcaggat gcaaccaatt acaactctat ttttgctaat cgatttgctg cttttgatga
    26761 gctactttca attctaaaaa ctaaatttgc atgtcgggtg ctttttgaag aaaccttggt
    26821 tttgccaaaa gttgggcgta gccgattaca tctgtgtaaa gatggctcac caagagtgat
    26881 taaagccgtt ggggtgcaac gtaatggcag tgaatttgta ttgctagagg tggatgcatc
    26941 ggatggggtg aaaatgcttt ctaccaaagt gttgagtggc gttgatagcg aaacatggcg
    27001 gaatgatttt gaaaagatac ggcgtggagt ggtgaagagc tcattgaatt ggccaaatag
    27061 tttgtttgat caattatatg gacaagacgg gcatagaggg gtgaatcatc caaaggggtt
    27121 gggggagctg caagtatcga gagaggatat ggaagggtgg gctgagagag tggttagaga
    27181 gcaatttacg cattaaagga atgactgaaa gagcctgtaa acccttttgt gtaagtgctt
    27241 ttgccggtca gttaaaggtg gccatttaaa cggtcaccaa attcgatcat aaaacggttc
    27301 atggccggct tccagttgcg gatcggcatc gtccatttct tggtcgccgc ctggatagcc
    27361 aggtacacca ccttcatcgc tgattcgtcc gtagggaaca ccttgagttc atattaatgg
    27421 aattttctac aaatagcctc cgtggttttg aggggggatt acagacgatc catagtagta
    27481 atccaatgag ttcttgagcg cggcgacatg tttggacgcc ttggcaaaaa ttagagcctg
    27541 cttgaagtgc aggcgagcac gtgcttggac gattgatcca ttcgcggtca aaaactcaat
    27601 cttggatgac agcgtgtcag gaaatccaat atcgaagtcc cgccccgtgt caaagtaggg
    27661 catttcatga tcaaaggacg aagcttccca ggccacttcc ctaatattcc agccgctaga
    27721 aacaagattt cgtacgactt catcaacttt tttgctttcc aacttgtcac aaagtgcata
    27781 ggcaagagct ttggcggcag acacgtgcgt ttcctcttaa acataggtcg aacggatacc
    27841 cgaggcaaag cggagcatta gtcccaaagc atctccggta gatccggagg ctccatactg
    27901 aaggcatggc atcttgggca ttgatatttg aaacttctct ctagatagca gggatttctt
    27961 ggccttgcct catgctgata gcttgtaaat atcacacgat ccttagcccc acaaagtgag
    28021 catactccgt agctttcatt tatagaaatc gtcgttagat tgtcgcagca tgtgcacgtt
    28081 ccgactattt gctcaggaag catcgccata ccttggccta ggtaaagaag atcagactcg
    28141 tagccacatc ggtggcaact gagcttgtac gcaatgccca tctggttgtc tcacctgaat
    28201 cgtaacgtca gagctattca tccatccatt cggcatcaat gtatgccact aagccgacca
    28261 gctgaccgtt tggactgaag tatgttgcct gcaccccact tgacatctca tcaataccac
    28321 cgcactgcgc taggcgccgg tcccacttgc ccacagaagc gtcaggatcg gctgagttcc
    28381 ccaagaccct tgtgagccat gcctccttta actcaacctc tcgaatgatt gcgtcggcca
    28441 gctcctccgc gttatctagt tcgacatagt ctggaattcg gacgtacgtt ctgaacacag
    28501 ctggatactt acttgggcga ttgtcataca tgacatcaac aatgtacccg tttgtgtaac
    28561 cgtctcttgg aggttcgtat gacactagtg gttcccctca gcttgcgact agatgttgag
    28621 gcctaacatt ttattagaga gcaggctagt tgcttagata catgatcttc aggccgttat
    28681 ctgtcagggc aagcgaaaat tggccattta tgacgaccaa tgccccgcag aagctcccat
    28741 ctttgccgcc atagacgccg cgcccccctt ttggggtgta gaacatcctt ttgccagatg
    28801 tggaaaagaa gttcgttgtc ccattgttgg caatgacgta gtagccggcg aaagtgcgag
    28861 acccatttgc gctatatata agcctacgat ttccgttgcg actattgtcg taattggatg
    28921 aactattatc gtagttgctc tcagagttgt cgtaatttga tggactattg tcgtaattgc
    28981 ttatggagtt gtcgtagttg cttggagaaa tgtcgtagtt ggatggggag tagtcatagg
    29041 gaagacgagc ttcatccact aaaacaattg gcaggtcagc aagtgcctgc cccgatgcca
    29101 tcgcaagtac gaggcttaga accaccttca acagatcgcg catagtcttc cccagctctc
    29161 taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt
    29221 atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat
    29281 ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct tcaagtatga
    29341 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
    29401 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
    29461 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
    29521 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
    29581 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
    29641 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
    29701 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
    29761 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
    29821 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
    29881 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
    29941 ggcgctcgat gacgccaact acctctgata gttga
//
LOCUS       DAJTYL010000042        28892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYL010000042 DAJTYL010000000
VERSION     DAJTYL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.042"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..404
                     /locus_tag="OO572_001804"
     CDS             <1..404
                     /locus_tag="OO572_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6660838.1"
                     /translation="VSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGT
                     NMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLE
                     HGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            426..1835
                     /locus_tag="OO572_001805"
     CDS             426..1835
                     /locus_tag="OO572_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6660839.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1813..2592
                     /locus_tag="OO572_001806"
     CDS             1813..2592
                     /locus_tag="OO572_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6660840.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2589..3449
                     /locus_tag="OO572_001807"
     CDS             2589..3449
                     /locus_tag="OO572_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6660841.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3597..4172)
                     /locus_tag="OO572_001808"
                     /pseudo
     CDS             complement(3597..4172)
                     /locus_tag="OO572_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4189..4449)
                     /locus_tag="OO572_001809"
     CDS             complement(4189..4449)
                     /locus_tag="OO572_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6660842.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4440..5711)
                     /locus_tag="OO572_001810"
     CDS             complement(4440..5711)
                     /locus_tag="OO572_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6660843.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5789..6151)
                     /gene="queD"
                     /locus_tag="OO572_001811"
     CDS             complement(5789..6151)
                     /gene="queD"
                     /locus_tag="OO572_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6660844.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6470..8269
                     /gene="cysJ"
                     /locus_tag="OO572_001812"
     CDS             6470..8269
                     /gene="cysJ"
                     /locus_tag="OO572_001812"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6660845.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8269..9981
                     /gene="cysI"
                     /locus_tag="OO572_001813"
     CDS             8269..9981
                     /gene="cysI"
                     /locus_tag="OO572_001813"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6660846.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10055..10789
                     /gene="cysH"
                     /locus_tag="OO572_001814"
     CDS             10055..10789
                     /gene="cysH"
                     /locus_tag="OO572_001814"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6660847.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11053..11205
                     /locus_tag="OO572_001815"
     CDS             11053..11205
                     /locus_tag="OO572_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6660848.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11399..12490
                     /locus_tag="OO572_001816"
                     /pseudo
     CDS             11399..12490
                     /locus_tag="OO572_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12636..13673)
                     /gene="iap"
                     /locus_tag="OO572_001817"
     CDS             complement(12636..13673)
                     /gene="iap"
                     /locus_tag="OO572_001817"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6660849.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13925..14833
                     /gene="cysD"
                     /locus_tag="OO572_001818"
     CDS             13925..14833
                     /gene="cysD"
                     /locus_tag="OO572_001818"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6660850.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14835..16262
                     /gene="cysN"
                     /locus_tag="OO572_001819"
     CDS             14835..16262
                     /gene="cysN"
                     /locus_tag="OO572_001819"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6660851.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16262..16867
                     /gene="cysC"
                     /locus_tag="OO572_001820"
     CDS             16262..16867
                     /gene="cysC"
                     /locus_tag="OO572_001820"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6660852.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16917..17240
                     /locus_tag="OO572_001821"
     CDS             16917..17240
                     /locus_tag="OO572_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6660853.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17434..17745
                     /gene="ftsB"
                     /locus_tag="OO572_001822"
     CDS             17434..17745
                     /gene="ftsB"
                     /locus_tag="OO572_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6660854.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17764..18474
                     /gene="ispD"
                     /locus_tag="OO572_001823"
     CDS             17764..18474
                     /gene="ispD"
                     /locus_tag="OO572_001823"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6660855.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18474..18953
                     /gene="ispF"
                     /locus_tag="OO572_001824"
     CDS             18474..18953
                     /gene="ispF"
                     /locus_tag="OO572_001824"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6660856.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18950..19999
                     /gene="truD"
                     /locus_tag="OO572_001825"
     CDS             18950..19999
                     /gene="truD"
                     /locus_tag="OO572_001825"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6660857.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19980..20741
                     /gene="surE"
                     /locus_tag="OO572_001826"
     CDS             19980..20741
                     /gene="surE"
                     /locus_tag="OO572_001826"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6660858.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20735..21361
                     /gene="pcm"
                     /locus_tag="OO572_001827"
     CDS             20735..21361
                     /gene="pcm"
                     /locus_tag="OO572_001827"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6660859.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21501..22640
                     /gene="nlpD"
                     /locus_tag="OO572_001828"
     CDS             21501..22640
                     /gene="nlpD"
                     /locus_tag="OO572_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6660860.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22703..23695
                     /gene="rpoS"
                     /locus_tag="OO572_001829"
     CDS             22703..23695
                     /gene="rpoS"
                     /locus_tag="OO572_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6660861.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23815..24222)
                     /locus_tag="OO572_001830"
     CDS             complement(23815..24222)
                     /locus_tag="OO572_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6660862.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24369..24962
                     /locus_tag="OO572_001831"
     CDS             24369..24962
                     /locus_tag="OO572_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6660863.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24962..25909
                     /locus_tag="OO572_001832"
                     /pseudo
     CDS             24962..25909
                     /locus_tag="OO572_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26182..26745)
                     /locus_tag="OO572_001833"
     CDS             complement(26182..26745)
                     /locus_tag="OO572_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660864.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26910..27161
                     /locus_tag="OO572_001834"
     CDS             26910..27161
                     /locus_tag="OO572_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6660865.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27163..>28892
                     /locus_tag="OO572_001835"
     CDS             27163..>28892
                     /locus_tag="OO572_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6660866.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYD"
BASE COUNT         7021 a         7183 c         7827 g         6861 t
ORIGIN      
        1 aagtgtccgg ctgctggagc tgcatccacg aaatctacga aagcgttatt aaccgtattc
       61 gtactgagtt cccgcacgcc gacgacatca ccatgctggg cggtcactcc tctcatagct
      121 atcagaacgg caccaacatg tacttcgtct atgactacaa cgttgttgac tgtaagccgg
      181 aagaggaaat cgacaagtac cacaatccgc tcaacaaaat catctgcgaa gaaaccattc
      241 gcctcggcgg ttcgatggtg caccaccatg gcattggtaa acatcgcgtt cactggagca
      301 agctggaaca cggcagcgcg tgggcgttgc tggaagggct gaaaaagcag ttcgatccta
      361 acggcattat gaatacgggt actatctatc cgattgaaaa ataatgtatc aggcagcgtt
      421 ccgcgatgac ggggcgttgc cttttcggct tctcaggcga gaagccgttc ttattaccgg
      481 acaatgaagg ggtaaagatg aacacttcac cggtgcgaat ggatgattta ccgcttaacc
      541 gttttcactg tcgaattgct acgctcactt tcggcgcaca cctgaccgac ggttatgttc
      601 taggcgtcat tggttacgcc attattcaac ttacgcccgc catgcaactg acgccgttta
      661 tggcgggaat gatcggcggc tcggcgctcc ttggtttgtt tcttggcagc ctggttcttg
      721 ggtggatctc cgaccatatt ggtcggcaaa aaatcttcac cttcagcttt ttgctgatta
      781 cgctcgcttc gttcttgcaa ttttttgcca ccacgccaga gcatcttatt gggctgcgca
      841 ttttgatcgg cattggtctg ggaggcgatt actcagtagg tcacaccttg ctggctgaat
      901 tttccccgcg ccgccatcgc ggtattttgc tgggcgcatt cagcgtggtg tggaccgtag
      961 gctatgtgtt ggcaagtatt gccggacatc actttatttc cgaaaacccg gaggcctggc
     1021 gctggttgct ggcatcggca gctctgcccg cgttgttgat tacgttatta cgctggggaa
     1081 cgccagaatc gccacgttgg ctactgtgcc aggggcgttt tgcagaagct cacgctatcg
     1141 tgcatcgcta ttttggtccc catgttttac tgggcgatga agtggtaacg gcgacccata
     1201 aacacatcaa aaccttgttc tcttcgcgtt actggcggcg cacggcgttt aacagcgtct
     1261 tctttgtctg cctcgtaatc ccatggtttg tgatttatac ctggctgcca actatcgccc
     1321 agactattgg tctggaagat gcgctgactg ccagcctgat gcttaatgcg ttgttaattg
     1381 tgggcgcgct gctgggatta gttctgacgc acctgctggc acatcgcaaa tttttgctgg
     1441 gaagtttttt gctgctggcg gcaacgctgg tagtaatggc ctgtttgcct tccggcagtt
     1501 cattaacgct gctgcttttt gttctcttca gcaccaccat ttcggcagtc agtaatctgg
     1561 tgggcatttt gcctgcggaa agttttccta ctgacattcg ctcgctggga gtcggttttg
     1621 ccaccgccat gagtcgactt ggcgcggcgg taagtactgg cctgctgccg tgggtgctgg
     1681 cgcagtgggg aatgcaagtc accttattgc tcctagcgac agtgttgttg gttggttttg
     1741 ttgtgacctg gctatgggca ccagaaacta aagccctccc gctggtggcg gcgggaaatg
     1801 taggaggtgc gaatgaacat tctgttagcg tttaaagccg aaccggatgc cggaatgctg
     1861 gcggaaaaag agtggcaggc ggcggctcag ggtaatagcg gaccggatgt ttcgctactg
     1921 cgaagtttac tcggtgctga tgaacaggcc gctgccgcgc tgctgttagc gcagagaaaa
     1981 aacggtacgc cgatgtcttt aaccgcgttg agcatggggg atgaacgggc gttgcactgg
     2041 ctgcgctatc tcatggctct tgggtttgag gaagctgtat tgctggagac ggcggcagat
     2101 ctgcgctttg cgccggaatt tgtcgctcgc catattgccg aatggcagcg tcagaatccg
     2161 ctggatctga taattaccgg cagccaaagc agcgaagggc agaatgggca aacgccattt
     2221 ttgctggcgg aaatgctggg ctggccctgc ttcacccagg tggagcgttt cactctcgac
     2281 gcgctgttta tcaccctcga acagcgtact gaacatgggt tgcgctgttg ccgggttcgc
     2341 ttgcctgcgg tgattgccgt gcgtcagtgc ggtgaagtgg ccttgccagt accgggaatg
     2401 cgccagcgaa tggctgcggg gaaagcagaa attatccgta aaactgttgc tgccgaaatg
     2461 ccagcgatgc agtgtctgca actggcaaga gcagagcaac gacgcggagc gacgctgatt
     2521 gatgggcaaa cggtagcaga aaaagctcaa aagctatggc aggactattt acgccaaagg
     2581 atgcagccat gaatatcgca attgtgacaa taaatcagga gaatgccgca atagcgagct
     2641 ggctggcggc gcaggatttc agcggttgta cgctggcgca ctggcaaata gaacctcagc
     2701 ccgtagtggc ggaacaggtg ctggatgcga tggtggaaca gtggcagcga acgcctgcgg
     2761 acgttgttct ctttccgccg ggaacgtttg gcgatgaact gtcgacccgg ctggcctggc
     2821 gtttacatgg tgcaagcatc tgtcaggtga cttcgttaga tattccaacc gtaagcatgc
     2881 gcaaatccca ctggggaaat gcgttaacgg cgacgctgca aacagaaaaa cgcccgttat
     2941 gcctttccct ggcgcgtcag gcgggagcag cgaaaaacgc cacgttgccc tctggaatgc
     3001 agcaactgaa cattgtccca ggtgcgcttc ccgactggct ggttagcaca gaggatctga
     3061 aaaatgttag ccgcgaccca ttagctgagg ccagacgggt gctggtggtg ggacagggcg
     3121 gagaggcaga taaccaggaa atcgttatgc tggcagaaaa gctgggagcc gaagtgggct
     3181 acagtcgggc gcgagtgatg aacggtggcg ttgatgctga aaaagtgatt ggtatttccg
     3241 gacatctgct ggcacctgag gtgtgcattg ttgttggcgc ttccggcgca gcagcgttaa
     3301 tggcgggcgt acgcaatagc aagtttatgg tggcgattaa tcatgacgcc agcgcagcgg
     3361 tattttcaca ggcagatatt ggcgtggttg atgactggaa agtggtgctg gaggcactgg
     3421 tgacaaacat tcacgcagaa taccaataac gtgctgccgg atgatcctgc ctacggttct
     3481 ggactgggga cctgtaggcc tgataagacg cgttaagcgt cgcatcaggc gctgaatgtc
     3541 ggatgtggcg tgcggccaac aaatcaaaac aaattcaatt ggttacgatt atcccgtcaa
     3601 agcaattttt tcgctaacgt ccagactccg gtattcgtgg tggaaagcgc cacgacaccc
     3661 gcgttaatcg cattacgcgc atcttcttca tcgcatacca gcccgccagc aatcagcggt
     3721 tggcggattt tttccgtcac ccagcccagc actttgggca tgcagcctgg caggatctca
     3781 atacaatccg gattcgattg cgcaacttac ttatcaatgt tgtgaaacga aatcgaatca
     3841 acaataaaca ggtgatgaat acagaaaaaa acttccgctc ttgccgcttt tagcatcgat
     3901 gctttggtgc tgataattcc atccgcctcg gtcaccagtt tcaaaaactg aatcaccact
     3961 tctttatttg acgcgccttc cagcaaatca acatgaataa aagcatattt cccggcgttc
     4021 ttaatctttt taacgatatt actgatggtg cagatattgc cgtacaacac ggaaataaac
     4081 tggcattcag aatcgatggc cagttgcagg ctggcattat ctttaacggc agcaatcacc
     4141 ggattctggc ggagcaggtg taagaggggc ataaattatc cttattattt atccaaagcg
     4201 gtaggtgatg ccgaatccgg aaggcggata gcgccactgt tgtagtgtgg attcgtcgca
     4261 cagcaaacgg caggtgccac actccaggca gccgcgatag tcaatacgta agtcaccttc
     4321 ctgtgtgaga gaaaaaagac ctaccggaca ggcgttaatc aaccgttgta ccatctgacg
     4381 ctcaacggag tcagccggaa caatgtgcgg cgcatcagca acgcgccaga gattacgggc
     4441 tacagacatc gcagactcct gataacatcg cccgccagat gccacaggcc atgacgacgt
     4501 aaatgatgcc agaataactg gcgcagcggt ggaacaggtt tatcaccctg atcccataaa
     4561 tcgcgggaaa tatcctgcat taacgcaggc cacgcccgat acctgccagg gcgttgcaaa
     4621 agcgccggaa catgctgata acgttgtaga acatcccata gcaggctgcg ctctacgttg
     4681 tgatgataaa gcgcaaacag attttgcggc tcgcggtgct ggcaggcgct tattagcgtt
     4741 tgtgccgccg cctgtgcgcc agtcagcgcc atatccatgc cgcgcacgga aattccggta
     4801 ttgacgcaac tgcgcaacgc atcgcccacc agcagccagc cgttaccggc gtattgcacc
     4861 ggcatactgt gcaagccacc ttctggcacc agatgcgcac catactccag tgattccgtg
     4921 tttttgataa gcgtgcgcac tgccggatgc gctttaaagc gagtcagcag ctcgcttgcc
     4981 ggaacacgac tttgcgtaag ggaagagagc gggcaaacaa tccctaacga gagcgtttgt
     5041 tgattagtat aaagaaatgc gccgccgggt aagtcatcac agattccgcc gctgaacagc
     5101 aacgctgcgc cttcgttatt ctccagatga aaacgttctt caatagcgga tgtttccagc
     5161 gacagcactt ctttgatccc caacgccatc gcttcgccag caggacgagt cactaacccg
     5221 tgacgttcag ccaggacgct gttggcacct tctgccagca ccacataacg ggcgcggaga
     5281 atatcgtcac cacaaataac gccacagact ctgccgtttt cttcatacag tgcatccacc
     5341 gtcgctccgg ggatgcattc gacaccttct ttttcggctt cggcaaccag ccacggatcg
     5401 aaccgtgcac gtaatacatt ccaggattca ccgccgggct gtaagctgga aaacgtcgtt
     5461 gcgccatccg gcgttaacag ggaaaggctt tcgtgagtga tgcgtcgttc aagaggcgcg
     5521 gtcagatgaa attgcgggag gagttccgcg agtgcatggg tatataaccg cctgccggaa
     5581 agatttttgc tgccggggat ttcagcgcgt tcgagtaaca aaacggataa atccgctcgc
     5641 gcgcagcgta acgcgcaagc ggtgcctgca ataccggcac caataataat aatgtcgcag
     5701 tcgtcttcca tgtcatgccc cagatatcca gaacacaaca ccctaacata gcgttactaa
     5761 agggaaattg accgccgaca cactcttatc attcgccgcg atagatacac cctgcggtgc
     5821 aggtttcttt taccatcacc gcacttaaca gcggcacaac gggtttaacc tgatcccaaa
     5881 tccattttgc taaaacttcg ctggttggat tttccagacc tggaatatca ttgagataat
     5941 ggtgatcgag gcgctcataa gttggcttaa acgccgcttt cagttcagcg aaatcgataa
     6001 tccagcccgt atgcggatcg acttccccgg taatttccag tcgcaccata aaggaatgcc
     6061 cgtgcaggcg accacattta tgcccttccg ggacatgtgg taagcggtga gcggcttcga
     6121 aggtgaaatc tttaaataac gtggtggaca tcataatttc tctacaggac gataaaaccg
     6181 ccgtagagta ccggaaaggc tgttttttcg cattatctaa aacgactttg ttgcgcaaaa
     6241 tcgctgattt atcttaatga ttggctaaat tcatttgttt ttcattaggt tggttaatct
     6301 attttgttgt taaagactat tgctaaaaca ggttagtcga tttggttatt agttatcgct
     6361 atcccgtctt taatccacac cgttcacccc gttaacctta ccttctcttc tgttttatgg
     6421 gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca tgacgacaca
     6481 ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc ttcaggcggc
     6541 cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct ggggcgtgct
     6601 caaccagcag cctgttgcgc ttgcagtgac gccagcgccc gccgcagaaa tgccgggtat
     6661 aacgattatc tctgcttcgc aaaccggcaa tgcgcgccgg gttgctgaag cattacgcga
     6721 tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact ataaattcaa
     6781 acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg aaggggaacc
     6841 gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc caaagctgga
     6901 aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt tctgccagtc
     6961 cgggaaagat tttgacagca agctggcgga actggggggt gaacgcctgc tcgaccgtgt
     7021 cgattctgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg tggttgatgc
     7081 gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg gcgcggtaaa
     7141 tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc tctctgttaa
     7201 ccagaaaatt actgggcgta actctgaaaa agacgttcgc catatcgaaa ttgacttagg
     7261 cgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc agaacgatcc
     7321 ggcactggtg aaagaacttg tcgaactgct gtggctgaca ggcgatgaac ctgtcactgt
     7381 cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac tgaccgtcaa
     7441 caccgccaac attgttgaga attacgccac gcttacccgc agtgaaacac tgctgccgct
     7501 ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgctgattg ttgacatggt
     7561 gcgtttctcc ccggcacagc ttgatgccga agcgctaatt aatctgctgc gcccgctgac
     7621 cccgcgtctc tattccattg cctcctcgca ggcggaagtc gagaacgaag tacacgtcac
     7681 cgttggtgtg gtgcgttacg acgtggaagg ccgcgcccgt gccggtggtg cctccagctt
     7741 cctcgctgac cgcgtggaag aagagggcga agtccgcgta tttatcgaac ataacgataa
     7801 cttccgcctg cccgctaacc tggaaacccc ggtgattatg attggcccag gcaccggtat
     7861 tgcgccgttc cgcgccttta tgcagcaacg cgccgctgac gaagcgccag gtaaaaactg
     7921 gctgttcttt ggcaatccgc attttacgga agacttcctg tatcaggtgg agtggcagcg
     7981 ctacgtcaaa gagggtgtgc tgacgcggat cgatctcgcc tggtcgcgcg accaaaaaga
     8041 aaaagtttac gtacaagata aactgcgcga acagggcgcg gagctgtggc gctggatcaa
     8101 tgacggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag acgttgagca
     8161 ggcacttctg gaagtgattg ccgaatttgg tggcatggac accgaagcgg cggatgaatt
     8221 tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat gagcgaaaaa
     8281 catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat gaagcttgaa
     8341 agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac cggcggcttt
     8401 aagggcgata acttcctgct gatccgcttc cacggcatgt atcagcagga tgaccgcgac
     8461 atccgcgccg aacgtgctga acagaagctg gagccgcgcc acgcgatgct gttgcgctgc
     8521 cgtctgccgg gtggagttat caccaccaaa cagtggcagg cgatcgataa atttgccggt
     8581 gaaaacacca tctatggcag cattcgcctg accaaccgcc agacgtttca gttccacggc
     8641 attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg tcttgatgcg
     8701 ctggcgactg ctaacgacat gaaccgtaac gtactctgca cctcgaaccc ttacgagtcg
     8761 cagctgcacg cagaagcgta cgagtgggcg aagaagattt ctgaacatct gctgccgcgt
     8821 acccgcgcgt atgcggagat ctggctcgat caggaaaaag tcgccaccac cgatgaagaa
     8881 ccgatccttg gtcagactta cctgccgcgt aagttcaaaa ccacggtagt gatcccgccg
     8941 cagaacgata ttgatctgca cgccaacgac atgaacttcg tggcgatcgc ggaaaacggc
     9001 aagctggtgg gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca cggtaacaag
     9061 aaaacctacg cccgcacggc gagtgagttt ggctatctgc cgctagagca tacgctagcg
     9121 gtggcggaag ccgtcgtgac aactcagcgt gactggggta accgaaccga tcgtaaaaat
     9181 gccaaaacca aatacacact ggagcgcgtg ggggttgaga cgtttaaagc ggaagtggag
     9241 cgtcgcgcgg ggatcaaatt tgaaccgatc cgcccgtatg aatttacagg acgcggcgat
     9301 cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt tatcgaaaat
     9361 ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaactg gcctgctgga gatcgcgaag
     9421 atccacaaag gtgatttccg cattacggcg aaccagaatc tgatcatcgc cggtgtgccg
     9481 gaaagcgaga aagcgaagat cgagaaaatt gccaaagaga gcgggttaat gaatgccgtc
     9541 acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc gctggcgatg
     9601 gcggaagcgg agcgtttcct gccgtctttt atcgacaaca tcgataattt aatggtgaaa
     9661 catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa cggttgtggt
     9721 cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcac cgggtcgcta caacctgcat
     9781 cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa catcaccgag
     9841 ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga gcgcgaagcg
     9901 ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt gctcgatccg
     9961 gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa gacgcgtgag
    10021 cgtcgcatca ggcaaggcaa acagtgagga atctatgtcc aaactcgatc taaacgccct
    10081 gaacgaactg ccgaaggtag atcgcattct ggcgctggca gaaactaacg ccgaactgga
    10141 aaaactggac gctgaaggcc gcgttgcctg ggcgctggat aatctgcccg gtgaatatgt
    10201 actttcttcc agctttggca ttcaggcggc ggtgagcctg catctggtga atcagattca
    10261 cccggatatt ccggtgatcc tcaccgacac cggctatctg ttcccggaaa cctaccgctt
    10321 tattgacgag ttaacggaca aactcaagct caacctgaaa gtgtaccgcg ctaccgaaag
    10381 cgccgcctgg caggaagcac gctacggcaa actgtgggaa cagggcgttg aaggcattga
    10441 aaagtacaat gacatcaaca aagtcgaacc gatgaaccgg gcgctgaaag aactgaatgc
    10501 gcaaacgtgg tttgctggcc tgcggcgtga acaatccggc agccgtgcca atttaccggt
    10561 gctggcaatt cagcgtggcg tatttaaagt gctgccgatt atcgactggg ataaccgaac
    10621 tatttatcag tacctgcaaa aacatggcct gaaatatcac ccattatggg acgaaggata
    10681 tttatcggtg ggcgataccc atacaacccg taaatgggaa cccggcatgg cggaagaaga
    10741 aacgcgtttc tttggcttaa aaagggaatg tggattacac gaagggtaaa tattgccgga
    10801 taaattaaac gcctctgtca gaaatgatgg gggcgttttt attttgcgag cagcattccg
    10861 gcatttaaag gtgaacatcg cagggaaaat taacaggtat agaatggcgt tgggtgcttg
    10921 aggctgtctg gcgttaagca ttcgcgaggt tccagatgga caaaagcccc aggcgatatt
    10981 tctatcaacc tgaggctcgc gttcgaacgt agcaattcgg atgttagtct cttctttgca
    11041 gggaggcaag acatgctgac aaaatatgcc cttgtggcaa tcatcgtact gtgttgtaca
    11101 gtactgggat tcacgttgat ggtaggtgac tcgttatgtg agttgagtat cagagaacgt
    11161 ggtatggagt ttaaggcagt tctcgcttac gaatcgaaga agtagccacc acgcgggggt
    11221 aaaaccccgc gtactgaatt gtgatggttt ggtctcctgc accctttttt taaatttata
    11281 ttttttatgt gaatatatag tctctattta ttattatgaa tttcaaatga aaatcattaa
    11341 tttattcata atgtaaatgt tttattggct gatatatatc atagatagtt tttaaaatat
    11401 gcgtaaatat cctttaagtt tactgaagga taaaaatatt gtgactttct ttgatttctg
    11461 gggaaaaaac cgacgtggcg agaaagaggg tatcgacgac tatcaccttc tttgctggca
    11521 tttgctggat gtggccgcaa tgggctattt aatggttaaa agaaattgct tcgggctggc
    11581 tgattatttt cgtcaattag ggatttctga caaggaacag gcagctcgat ttttcgcctg
    11641 gttgctgtgc tggcacgata ttggaaaatt tgcccgctct tttcaacaac tttacctgcc
    11701 ccctgaattc aagattcagg aaggtgccag aaaaaattac gaaaaaatct ctcattcaac
    11761 gctgggctac tggttgtgga atcattattt aagtgaatgt caggagttac ttccttcatc
    11821 ttcgctctct ccgcgcaaac ttaggcgagt gatagaaatg tggatgcccg tgacgacagg
    11881 gcatcatggt cggccccctg atcgtatgga tgaattggat aattttctgc ctgaagacaa
    11941 agctgcagca cgagattttc tccttgaaat caagccactg tttccgcgaa tagagatccc
    12001 cacattctgg gatgatgacg agggcgttga acttataaaa caactttcct ggtatatctc
    12061 tgcaaccgtc gtacttgcag actggacagg gtcgtcaacg cgattttttc cacgcgtcgc
    12121 acaggcaatg gatattgaag attactggca aaaggcttta gttcaggctc aaaacgcatt
    12181 aaccgtcttt ccgccacaag cagaaaccgc gcctttcacc ggaattaata cgctgtttcc
    12241 ttttattgag aatacgacac cgttacagca aaaggtgctg gatctggata tcaatcagcc
    12301 aggaccacag ttgtttattc tggaagatgt gaccggcgca ggtaaaacag aagcggcgct
    12361 tatcctggcg cacaggttga tggctgcggg taacgctcag gggctgtttt ttggcctgcc
    12421 aacaatggca acggccaatg ccatgtacga tcgactggtc aaaacctggc ttgctttcta
    12481 ttcgccagag gtgttccccg ctccagcgga gataaaccgg cgcaatacgg tgcggttttc
    12541 ccggaagtaa accggacgca ctggatgcga tgatgggtat cacttagaat tccccgcccc
    12601 tgcggtagaa ctcccagctc ccattttcaa acccatcaag acgccttcgc cagctccttc
    12661 accaacggta gcattatccg cataacgtca cggcagcgac gttctattct tccaggaagt
    12721 gccttatcaa tatgttgttg attatccagt cttacgtcat gccagctatt tcccgccggg
    12781 aaggcaggtg tttttgcgcg ttgctgataa ccatccttat ttccaagatt ccagttagtc
    12841 gcttccaccg aaagtacagc aatgcccgct ttgtcgaata tttctgcgtc attacaaccc
    12901 ccagtgcctt tcggataatt tttattcaaa cccggattgg tcgttgcggc tattccgtga
    12961 ctgcgtgcaa ttgccagcgc cctgtcgcgc gttaatttcc ttactgcttc aggggttttt
    13021 acaccgctgt tgaaatacaa tttatcgcca acaattaagt tatcgagatt aatcaccagc
    13081 agcgtatttt tcttttcggt gtcactcatc cgcttgagta aattctcagc gcctaatttt
    13141 ccttcctctt cgccgctggt cgccacaaat cgaataccat actcggtagg cgtatttttc
    13201 aggcgttctg ccagttccag catgacacct aaacctgcgg cgttatcatc tattccttgt
    13261 aacgtcagtc cgccgagatt ggcatctgcg tcagcatcgc tcagcggggc gtaagtatcc
    13321 agatgcgcca taatgatgat ctgctgcggc gctttgcctt catgagcggc aatcaccgta
    13381 cttcctgtca cgttatgcca gttcttgcga ttatcgcggg cggtataaat ataccgacta
    13441 ttaaatgtcc gaatatcact gcgataaccc atttgctgaa actgttggcg aatataatcg
    13501 gcagataaca tttctgctgg agttccggtc atgcgtcccg gaaagaaagt agcaatatgt
    13561 cgtgcctggg tattagcaaa atcgccaggt ttaggtgacg aggcgtgtac ggggagaata
    13621 aagcatacgc cgagcgccag ggcagcggta cggtggcgca atgcggaaaa catagtgagt
    13681 ccttaaatac catgcaaatt tttttaccgc catagtatga aactgccgct gcgctaaaac
    13741 aatttcaaat cttcctaaac gcccgaaatc cggtgcctta agcacttttt gatattagct
    13801 ttgccaaatc gttattccgt taaggaacta ctcattctaa ttggtaattt cattcgttct
    13861 cttacgctcc ctatagtcga aacatctgat ggcaagaaaa tagcggtatt gcaaaggaac
    13921 ggttatggct caaaaacgac ttactcacct gcggcaactg gaggcggaaa gcatccacat
    13981 tattcgcgag gtggcggcag aattctcaaa tccggtgatg ctctactcta tcggtaaaga
    14041 ttccagcgtc atgctgcatc tggcgcgcaa ggcgttttat ccaggtacgc tgcctttccc
    14101 gttgctgcat gtcgataccg gctggaaatt ccgcgagatg tatgagttcc gcgatcgtac
    14161 ggcgaaagcc tacggctgcg agctgctggt gcataaaaac ccggaaggcg tggcgatggg
    14221 gattaatcca ttcgtgcacg gcagcgcgaa acataccgat attatgaaaa ctgaaggcct
    14281 gaaacaggcg ctgaacaaat acggttttga tgccgccttc ggtggtgcgc gccgtgacga
    14341 agagaaatcc cgcgctaaag agcgaatcta ctctttccgt gaccgcttcc atcgctggga
    14401 tccgaaaaat cagcgcccgg agctgtggca caactacaac gggcaaatta acaaaggcga
    14461 aagcatccgc gtcttcccgc tctctaactg gaccgagcag gatatctggc aatacatctg
    14521 gctggaaaat atcgacattg tgccgctgta tctcgctgcg gaacgtccgg ttctggaacg
    14581 cgacggtatg ttgatgatga ttgatgacaa ccgtatcgac ctgcaaccgg gcgaagtgat
    14641 caaaaaacgg atggtgcgtt tccgtacgct gggctgctgg ccgctgaccg gtgcggtgga
    14701 gtcaaatgca caaacactgc cggaaatcat cgaagagatg ctggtttcca ccaccagtga
    14761 acgtcagggt cgcgtgattg accgcgacca ggcgggatcc atggagctga aaaaacgtca
    14821 ggggtatttt taagatgaac accgtacttg cacaacaaat cgccaatgaa ggcggcgtcg
    14881 aagcctggat gattgcgcaa caacataaaa gcctgctgcg ttttctgacc tgtggtagcg
    14941 tcgatgacgg caaaagtacc ctgattggtc gtctgctgca cgatacccgc caaatctacg
    15001 aagatcagct ctcatcgctg cataacgaca gtaagcgtca cggaacccag ggcgaaaagc
    15061 tggatctggc tctgctggtg gacggcctgc aagctgagcg cgaacagggc atcactattg
    15121 atgtggccta ccgctatttc tctaccgaga agcgtaaatt tattatcgcc gacaccccag
    15181 ggcacgagca gtacactcgc aatatggcga ctggcgcatc gacatgtgaa ctggcgatct
    15241 tactgattga tgcccgtaaa ggcgtgctcg atcaaacccg tcgtcacagt tttatctcca
    15301 cactgttggg gatcaaacat ctggtcgtgg cgatcaacaa aatggatctg gtggattaca
    15361 gtgaagagac gttcacccgt attcgtgaag attatctgac ctttgccggg cagctgccgg
    15421 gtaatctgga tatccgcttt gtgccgctct ccgcactgga aggtgacaac gtggcttcgc
    15481 aaagtgaaag tatgccgtgg tacagcggtc tgacactgct cgaagtgctg gaaaccgtgg
    15541 agatccagcg agtggtggat gcccagccaa tgcgcttccc ggtgcagtac gttaaccgcc
    15601 caaatctcga ttttcgtggc tacgccggaa tgctggcatc cggtcgcgtg gaagtcgggc
    15661 aacgtgtaaa agtgctgccc tctggtgtgg aatcaaacgt cgcgcggatc gtgacttttg
    15721 atggtgatcg cgaagaagtc tttgccggag aagcgatcac cctggtgctg acggatgaga
    15781 tcgatatcag ccgtggtgat ctgctgctgg cggcagacga agcgttaccg gcggtgcaga
    15841 gtgcgtcggt ggatgtggta tggatggcgg aacagccgct ttccccgggc cagagttatg
    15901 acatcaaaat tgccggtaag aagacgcgtg ctcgtgttga tggcattcgt tatcaggttg
    15961 atattaataa ccttacccaa cgcgaagttg aaaacctgcc gctgaacggc atcggcctgg
    16021 tggatctcac ttttgacgag ccactggtgt tagatcgcta tcagcaaaat ccggttaccg
    16081 gtgggctgat ttttatcgat cgcctgagca atgtgaccgt gggtgccggt atggtgcacg
    16141 agccagttag ccaggcaaca gctgcgtcat ctgaattcag tgcattcgaa ctggaattga
    16201 atgccctggt tcgtcgccac tttccgcact ggggcgcgcg cgatttgctg ggggataaat
    16261 aatggcgctg catgacgaaa acgtcgtctg gcatagtcat ccggtcactg tgcaacaacg
    16321 cgaactacac cacggtcatc gtggtgtagt gctgtggttt accggcctct ccgggtccgg
    16381 taaatcaacg gtcgccgggg cgctggagga ggcgttacat aaactcagcg tcagtacgta
    16441 tctgctggat ggcgacaatg ttcgccacgg attatgcagc gatctcggtt ttagcgatgc
    16501 cgatcgtaaa gagaatatcc gtcgcgtcgg tgaagtggcg aatttgatgg ttgaagccgg
    16561 actggtggtg ctgaccgcat ttatctcgcc acaccgcgcc gaacgccaga tggttcgcga
    16621 acgcgtagga gaagggcgct ttattgaagt gtttgtcgat acgccgctgg cgatttgcga
    16681 agcccgcgat ccaaaaggct tatataagaa agcgcgtgcc ggtgaactgc gcaactttac
    16741 gggaatagat tccgtttacg aagcgcctga atcggcagaa attcatctca atggtgaaca
    16801 attagtaaca aatttggtac agcaattatt agatctgttg agacagaacg atattatcag
    16861 atcctgagac accaccgggt ttccatgccc ggttcgtcaa agtaacagga tcaaatatgc
    16921 gtaatagcca taacattaca ctaacaaata acgacagcct tactgaggat gaagaaacta
    16981 catggtcact gcctggtgcc gtggtcggtt ttatctcctg gttatttgcg ctggcgatgc
    17041 cgatgttgat ttatggctct aacacgctgt tcttctttct ctacacctgg cctttctttc
    17101 tggcactgat gcctgtcgcg gtagtggtgg ggattgcgct gcattcattg atggacggaa
    17161 agctacgcta cagtattgtt ttcaccctgg tgacagtagg cattatgttt ggcgcactgt
    17221 ttatgtggct actgggctaa tttgtacttt cttgtctcct ctgttcataa ttcaaaccgt
    17281 aactaataat gagattatgt tctgcacgcc ctgggtatac gtaacaatgg gcaaatgtgg
    17341 tacatttgcc cgcgttgtcg cggtatcccc aacagaggat gtagagtcgt cttcggatgc
    17401 atgggatgat gatgcagttt ttcagggggc aggatgggta aactaacgct gctgttgctg
    17461 gctattctgg tctggctaca gtattcgctg tggttcggta agaacggtat acatgactat
    17521 acccgcgtca atgatgatgt ggcggcactg caagctacaa acgcgaaact taaagcgcga
    17581 aacgatcaac tttttgccga aattgacgat ctcaatggcg gccaggaggc gctcgaagag
    17641 cgtgcgcgta atgaactcag catgaccagg ccgggcgaaa ctttttatcg tctggtgcct
    17701 gacgcgtcga agcgcgcaca gtctgcgggg caaaacaatc gataaatcag cccgggaatt
    17761 aacatggcaa ccactcattt ggatgtttgc gccgtggttc cggcggccgg atttggccgt
    17821 cgaatgcaaa cggaatgtcc taagcaatat ctctcaatcg gtaatcaaac cattcttgaa
    17881 cactcggtgc atgcgctgct ggcgcatccc cgggtgacac gtgtcgtcat tgccataagt
    17941 cctggcgata gccgttttgc acaacttcct ctggcgaatc atccgcaaat caccgttgta
    18001 gatggcggtg atgagcgtgc cgattccgtg ctggcaggtc tgaaagccgc tggcgatgtg
    18061 cagtgggtat tggtgcatga cgccgctcgt ccttgtttgc atcaggatga ccttgcgcga
    18121 ttgttggcgt tgagcgaaac cagccgcacg ggagggatcc tcgccgcacc agtgcgcgat
    18181 actatgaaac gtgccgaacc gggcaaaaat gccattgctc ataccgttga tcgcaacggc
    18241 ttatggcacg cgctgacgcc gcaatttttc cctcgtgagc tgttacatga ctgtctgacg
    18301 cgcgctctaa atgaaggcgc taccattacc gacgaagcct cggcgctgga atattgcgga
    18361 tttcatccgc agttggtcga aggccgcgcg gataacatta aagtcacgcg cccggaagat
    18421 ttggcactgg ccgagtttta cctcacccga accatccatc aggagaatac ataatgcgaa
    18481 ttggacacgg ttttgacgta cacgcctttg gcggtgaagg cccaattatc attggtggcg
    18541 tacgcattcc ttacgaaaaa ggattgctgg cgcattctga tggcgacgtg gcgcttcatg
    18601 cgttgaccga tgcattgctt ggcgcggcgg cgctggggga tatcggcaaa ctgttcccgg
    18661 ataccgatcc ggcatttaaa ggtgccgaca gccgcgagct gctacgcgaa gcctggcgtc
    18721 gtattcaggc gaagggttat acccttggca acgtcgatgt cactatcatc gctcaggcac
    18781 cgaagatgtt gccgcacatt ccacaaatgc gcgtgtttat tgccgaagat ctcggctgcc
    18841 atatggatga tgttaacgtg aaagccacta ctacggaaaa acttggattt accggacgtg
    18901 gggaagggat tgcctgtgaa gcggtggcgc tactcattaa ggcaacaaaa tgattgagtt
    18961 tgataatctc acttacctcc acggtaaacc gcaaggcacc gggctgctga aagccaatcc
    19021 ggaagacttt gtggtggtgg aagatttggg ctttgagcct gatggtgaag gtgagcatat
    19081 tctggttaga atcctcaaaa acggctgcaa tacccgtttt gtggcggatg cactggcgaa
    19141 attcctgaaa attcattccc gtgaagtcag cttcgctggg caaaaagaca aacatgctgt
    19201 aacggaacag tggttatgtg ctcgcgtgcc gggcaaggaa atgcccgatc tgagcgcctt
    19261 tcaactggaa ggctgccagg tgctggagta tgcgcggcac aagcgcaagc tgcgtttagg
    19321 ggcgctgaaa ggtaacgcct ttaccctggt tctgcgcgaa gtgagcaatc gcgatgacgt
    19381 tgaacaacgt ctgatcgata tttgcgtaaa aggtgtaccg aactacttcg gtgcccaacg
    19441 ttttgggatt ggcggtagca acttgcaggg cgcgctgcgc tgggcgcaaa ccaatactcc
    19501 ggtgcgcgat cgcaataaac ggagtttttg gttgtcggca gcccgcagtg cgttgtttaa
    19561 tcagattgtt gctgagcgcc tcaaaaaagc agacgttaat caagttgttg acggcgatgc
    19621 gctacaatta gccggacgtg gtagctggtt tgtagcaacc accgaagaac tggcggaatt
    19681 acagcgtcgc gtcaacgata aagagctgat gataaccgcc gcattgccgg gcagtggcga
    19741 atggggaact cagcgtgaag cgctggcatt cgaacaagca gctgtcgccg cagaaactga
    19801 attacaagct ttactggtgc gcgaaaaagt tgaagccgcg cgcagagcga tgctgctgta
    19861 tccgcaacaa ttaagctgga actggtggga tgacgtcacc gtagagatcc gtttctggct
    19921 tccggcgggt agttttgcaa ccagcgttgt cagggaactt atcaacacaa caggtgatta
    19981 tgcgcatatt gctgagtaat tatgacgggg tacatgcacc cggtatacaa acgctggcga
    20041 aagccttgcg tgagtttgct gacgttcagg tggtcgcccc cgatcgtaac cgcagcggcg
    20101 cttcaaattc tctgacactg gaatcctccc tgcgcacgtt tacctttgaa aatggtgata
    20161 ttgctgtgca aatgggaacc ccgaccgatt gtgtctatct tggcgtgaat gctctgatgc
    20221 gtccgcgccc ggacattgtt gtgtccggaa ttaacgccgg gccgaatctg ggggatgatg
    20281 ttatttattc cggtacggta gccgccgcga tggaaggccg tcatttaggt tttccggcgc
    20341 tggccgtctc gcttgacggg cataaacatt acgacactgc cgcggcggta acctgttcaa
    20401 ttttgcgcgc actgtgtaaa gagccgctgc gcaccggacg tattcttaat attaacgttc
    20461 aggatttacc cttggatcaa atcaaaggta ttcgcgtgac gcgctgcggt acacgacatc
    20521 cggcagatca ggtgatcccg cagcaagatc cgcgcggcaa tacgctgtac tggattggcc
    20581 cgccgggcgg taaatgtgat gccggtccgg ggaccgattt tgctgcggta gatgagggct
    20641 atgtctccat cacgccgctg catgtggatt taactgcgca tagcgcgcaa gatgtggttt
    20701 cagactggtt aaacagcgtg ggagttggca cgcaatggta agcagacgcg tacaagcact
    20761 tctggatcaa ttacgtgcgc aaggtattca ggatgagcag gtgctgaatg cacttgccgc
    20821 cgtgccgcgt gaaaaattcg ttgatgaagc gtttgaacaa aaagcctggg acaatatcgc
    20881 cttgccgata ggtcaggggc agacaatttc gcagccgtat atggtggcgc gaatgaccga
    20941 attactcgag ctgacgccgc agtcgcgggt gctggaaatt ggcactggtt cgggatatca
    21001 aacggcaatc ctggcgcatc ttgtccagca tgtttgctcg gttgaacgga ttaaaggctt
    21061 gcagtggcag gcacgtcgcc gcctgaaaaa tcttgattta cataatgttt caacccgtca
    21121 tggtgatgga tggcaaggtt ggcaggcacg tgcgccgttt gacgctatca ttgttacggc
    21181 ggcaccgccg gaaattccaa ctgcgctaat gacgcagctg gacgaaggcg ggattctcgt
    21241 cttacccgta ggggaggagc accagtattt gaaacgggtg cgtcgtcggg gaggcgaatt
    21301 tattatcgat accgtggagg ccgtgcgctt tgtcccttta gtgaagggtg agctggctta
    21361 aaacgtgagg aaatacctgg atttttcctg gttattttgc cgtaggtcag cgtatcgtga
    21421 acatcttttc cagtgttcag tagggtgcct tgcacggtaa ttatgtcact ggttattaac
    21481 caatttttcc tgggggataa atgagcgcgg gaagcccaaa attcaccgtt cgccgcattg
    21541 cggctttgtc actggtttcg ctatggctgg caggctgttc tgacacttca aatccaccgg
    21601 caccggtcag ctccgttaat ggcaatgcgc ctgcaaatac taattctggt atgttgatta
    21661 cgccgccgcc gaaaatgggg acgacgtcta cagcgcagca accgcaaatt cagccggtac
    21721 agcagccaca aattcaggct actcaacaac cgcaaatcca gccagtgcag ccagtagctc
    21781 agcagccggt acagatggaa aacggacgca tcgtctataa ccgtcagtat gggaacattc
    21841 cgaaaggcag ttatagcggc agtacctata ccgtgaaaaa aggcgacaca cttttctata
    21901 tcgcctggat tactggcaac gatttccgtg accttgctca gcgcaacaat attcaggcac
    21961 catacgcgct gaacgttggt cagaccttgc aggtgggtaa tgcttccggt acgccaatca
    22021 ctggcggaaa tgccattacc caggccgacg cagcagagca aggagttgtg atcaagcctg
    22081 cacaaaattc caccgttgct gttgcgtcgc aaccgacaat tacgtattct gagtcttcgg
    22141 gtgaacagag tgctaacaaa atgttgccga acaacaagcc aactgcgacc acggtcacag
    22201 cgcctgtaac ggtaccaaca gcaagcacaa ccgagccgac tgtcagcagt acatcaacca
    22261 gtacgcctat ctccacctgg cgctggccga ctgagggcaa agtgatcgaa acctttggcg
    22321 cttctgaggg gggcaacaag gggattgata tcgcaggcag caaaggacag gcaattatcg
    22381 cgaccgcaga tggccgcgtt gtttatgctg gtaacgcgct gcgcggctac ggtaatctga
    22441 ttatcatcaa acataatgat gattacctga gtgcctacgc ccataacgac acaatgctgg
    22501 tccgggaaca acaagaagtg aaggcggggc aaaaaatagc aaccatgggt agcaccggaa
    22561 ccagttcaac acgcttgcat tttgaaattc gttacaaggg gaaatccgta aacccgctgc
    22621 gttatttgcc gcagcgataa atcggcggaa ccaggctttt gcttgaatgt tccgtcaagg
    22681 gatcacgggt aggagccacc ttatgagtca gaatacgctg aaagttcatg atttaaatga
    22741 agatgcggaa tttgatgaga acggagttga ggtttttgac gaaaaggcct tagtagaaga
    22801 ggaacccagt gataacgatt tggccgaaga ggaactgtta tcgcagggag ccacacagcg
    22861 tgtgttggac gcgactcagc tttaccttgg tgagattggt tattcaccac tgttaacggc
    22921 cgaagaagaa gtttattttg cgcgtcgcgc actgcgtgga gatgtcgcct ctcgccgccg
    22981 gatgatcgag agtaacttgc gtctggtggt aaaaattgcc cgccgttatg gcaatcgtgg
    23041 tctggcgttg ctggacctta tcgaagaggg caacttgggg ctgatccgcg cggtagagaa
    23101 gtttgacccg gaacgtggtt tccgcttctc aacatacgca acctggtgga ttcgccagac
    23161 gattgaacgg gcgattatga accaaacccg tactattcgt ttgccgattc acatcgtaaa
    23221 ggagctgaac gtttacctgc gaaccgcacg tgagttgtcc cataagctgg accatgaacc
    23281 aagtgcggaa gagatcgcag agcaactgga taagccagtt gatgacgtca gccgtatgct
    23341 tcgtcttaac gagcgcatta cctcggtaga caccccgctg ggtggtgatt ccgaaaaagc
    23401 gttgctggac atcctggccg atgaaaaaga gaacggtccg gaagatacca cgcaagatga
    23461 cgatatgaag cagagcatcg tcaaatggct gttcgagctg aacgccaaac agcgtgaagt
    23521 actggcacgt cgattcggtt tgctggggta tgaagcggca acactggaag atgtaggtcg
    23581 tgaaattggc ctcacccgtg aacgtgttcg ccagattcag gttgaaggcc tgcgccgttt
    23641 gcgcgaaatc ctgcaaacgc aggggctgaa tatcgaagcg ctgttccgca agtaagtaag
    23701 catctgtcag aaaggccagt ctcaagcgag gctggccttt ttcttttgtt tgggaagctt
    23761 gtgtttttgt cgcctggata agacacgtca gcgtcctacc cggcaatcag cattctatgt
    23821 gctcatcatc ttgcgcacca actgcataaa cagctcctgc tcttctccac tcaaacgccc
    23881 caaaaattcc tcatccacgc tgtcaccaat aggtatcgcc gcagcaagta gcttttctcc
    23941 ttcggcagtc agccagacaa agcgtcgccg cttgtcagcg acgtcatgtt cacgtctgac
    24001 taagccacga ttttccattc ttgccaacat ttctgctagc gttgctttgg tgctgactgc
    24061 tgcttctatc agtgccacct gttcaatacc aggtttatca gcaatggcgc gcatcaccgc
    24121 gtactgtggt ttggtcaggt caggtaactc gtgctgccag cgagccgtgt gctgctgaaa
    24181 aagctgtcgt aactgatgga acgctttatt tcgtaacgcc atgtaaactc cgggctaatt
    24241 ctctgtcgct atcatagcgg ttttttagtg acgaagaaga tgttgttact tttcaatcct
    24301 tgccgctgtg gaaatgaagg agtatgttaa taataacgtt cgtatacgaa taattaagag
    24361 aataagcaat gaaactgatc gtcgggatga caggggctac cggtgcgcct cttggtgtgg
    24421 cattactgca agcgctgcgt gagatgccga atgtcgagac tcatctggtg atgtcgaagt
    24481 gggcgaaaac caccattgaa ctggaaacgc cttacagcgc tcgcgatgtt gctgccctcg
    24541 cagacttcag ccataacccg gcggatcagg cggcgatcat ctcatccggt tcttttcgta
    24601 ccgacggcat gatcgttatt ccgtgcagta tgaaaacgct cgccggtatc cgcgctggtt
    24661 acgccgatgg cctggtaggg cgcgcggcgg acgtcgtgct caaagaaggc cgcaaactgg
    24721 tgctggtgcc gcgtgaaatg ccgcttagca ccatccatct cgaaaatatg ctcgcacttt
    24781 cacgcatggg cgtggcgatg gtgccgccga tgcctgcctt ttataaccat cccgaaacgg
    24841 tagatgacat tgtccaccat gtggtagccc gcgtgctgga tcaatttggc ctcgaacatc
    24901 cccacgccag gcgctggcaa ggattgccgc aggcccggaa tttttctcag gagaatgaat
    24961 aatggcattt gatgatttac gcagcttttt acaggcgctt gatgaccacg gccagttact
    25021 gaaaatcagc gaagaagtga acgctgagcc ggatctggca gcagcagcta acgccaccgg
    25081 gcgtatcggc gacggcgcgc ccgcgctgtg gtttgataat attcgcggct ttaccgatgc
    25141 ccgcgtggcg atgaatacca tcggttcctg gcagaaccac gcgatttccc ttggcctgcc
    25201 accaaacacc ccggtcaaaa agcagattga tgagtttatc cgccgctggg ataacttccc
    25261 gattgccccg gagcgccgcg ccaatccagc ctgggcgcag aacaccgtcg atggcgaaga
    25321 gatcaacctg ttcgatattc tgccgctgtt tcgcctgaat gatggcgatg gcggtttcta
    25381 tctcgacaaa gcgtgcgtgg tttcccgcga tccgctcgac ccggataact tcggcaagca
    25441 gaacgtcggc atctaccgca tggaagtgaa gggcaagcgt aagctcggcc tgcaaccggt
    25501 gccgatgcac gatatcgccc tgcatctgca taaagcagaa gagcgcggtg aagatctgcc
    25561 gattgcgatc acgctcggta acgatccgat catcacgctg atgggggcca cgccgctgaa
    25621 atacgatcag tctgagtatg aaatggcagg cgcgctgcgt gaaagcccgt acccgatcgc
    25681 caccgctccg ctgaccggtt ttgatgtgcc gtggggttca gaagtgatcc tcgaaggggt
    25741 tatcgaaagc cgtaaacgcg aaatcgaagg gccgttcggt gagtttaccg ggcactactc
    25801 cggtgggcgc aacatgaccg tggtgcgcat cgataaagtc tcttatcgca gcaaaccgat
    25861 tttcgaatca ctctatctcg gtatgccgtg gaccgaaatc gactacctat gactaggtaa
    25921 tttattcaag ataaccggga agagattaaa aaagacggga atagattgga atcaacaagt
    25981 taggaactca aggttttcaa gataaagcga gtccaaaaat gttaaacggt tcgatatcaa
    26041 taaaacattc aagataactc acaggttctt acaatagcct ttaaagaccg ttcaaaaacg
    26101 attcaggtgt ttattatagc gctgatgggt gcaagcgcca aaaccaatgt aattttagca
    26161 cagctaggca cttgcactgg gttatgcgct ggctgctttc tgaggtagat ttaagtgatc
    26221 gcccttagct tcggacaacg cgtaaatctc tctaaccttt tcgtctggca aattaccaag
    26281 gcttaacgct atggctctac gaactccaac tagagcgtgc aactcttgga tgtgctcatc
    26341 cagaagaatg agtaattgct ccgctccttt tctcccaaga acatcagcta cttgttgttt
    26401 ttcttgtggt ttaagaaatg acaagaacat tagaatttga gcatcaacat tatccgcatt
    26461 ctcttttttg ggttctttgg aatcttttag ttcaacatca ccctctcccg tcatgagcca
    26521 ttccaaggta acattttcgc tttctgctat cttttttaac tgatacgggc gtggtatcgg
    26581 tgcgtaatcc cgcctgttgt aataattttt taaagtgcct tcattgatcc cccatgcccg
    26641 agcagccgca tttcttgaag ggtatcgttc tactaaccta aagattcttt cgataatcgg
    26701 ttcttttcca tcggcaggaa aagaaccttg gggtgctttt ctcattcttg gttcctttct
    26761 atctaattca ttgataaata ataaaattaa aaaataaacc ataaaaacac caaagaaccc
    26821 tttgcaggga atctttggta tcttaatctt tatccatacg gatatccgac cggatatccg
    26881 cgcgggtaat tttgtaggtt aaaggaaaga tgagacaaag agaagatagt gaccgcgatt
    26941 ggccttcgct gaagattcag ctggcgctca aagataacgg gtggtcttcc ttccgggagc
    27001 tttcactcca tgctggttat gctcaggatt cactgaaagc ggtagttcgc acaccaaaca
    27061 aaaaatacga acgcgtaatt tctgacgcta taggtgtacc gccagaaatc atctggccca
    27121 gccggtatcg taacgatgga tttatcagga aggcgtcgtg atatgcagtc gtacgttacc
    27181 gtaaatgacc tgctcggcgt accaggaatg ccagccacca caaaaggcat ccgacaggcg
    27241 ttacagcgtt tttctcgtga tcttggtgac gttagtcgcc gtcgggaagg cacaaaagcc
    27301 attgagtacc acattgattg tctgcctgaa ataacacgaa aggcactgcg tgaacgctat
    27361 gtggaacagc tggtggctac agagaacaat gtttctgaag tgaaagctgt taccagaaaa
    27421 acacgcaatc ctgacgctgt ccaggcaatc gaagcatatc gcggttctcc acaactgatg
    27481 gaagaacgcc tgaatgcgct gaccgaaaac cagcgctggg tatctgaagc aagagctgcg
    27541 ctggtggtgg aagtgctgaa gctggaaagc gccggtaacc ccgggcgact gaaagccatt
    27601 aactttcttg ttgaaaaagc ccgtaaaggt gagctgccgg agcgcctgca acaggccgca
    27661 gttaacgcca atgcaaaacg tggcgctaat cgtactatca gccgcgatcc actttaccag
    27721 tgggttctga aatacaacca gtcgcagaac gccgccgaac ggctgctgtt actggcaccg
    27781 ggaaaacgcg acgaaataaa accggaagaa atcagctggc tgcctgaatt tctggcgcag
    27841 tatcgccagg tgaacggcag acccatgtca gaagcctacg aggattttgt cgcagaatgg
    27901 cagcgtcgcc atgcggacga accgtacatg ctggaggtga tgccttccta tgatgttgtc
    27961 cggtatgcca tgaaaaaact gccggaagtg gtgaaacaaa aagggcgcgt taccggcagt
    28021 gaataccgtc agcttgaagg attcacccgc cgcgactgga cagctatgcc ggtgaattat
    28081 gtctggattg gtgacggtca cggtatgaag ctgaaatgtg cgcatccgat ccacgggcgt
    28141 ccattctcac cggaagtcac gtttgtgatc gatggtggaa cacggtttgt tgttggctgg
    28201 agtcttgacc ttgccgaaaa cgtgttcgcc gttgccggtg cgatacagca cggcattcgc
    28261 aaccacggaa aaccgttcct gtattactcg gataacggtt ccggtgaaac ggctgacatg
    28321 ctggacaaag aggtcgtggg tattctgcca cgcctgggga ttaaacaccc taccggtatt
    28381 gccggtaatc cacagggacg cggcattatt gaacgcctga accggacact gccgatgcgt
    28441 atagcgagga ggtatcgcac ctattttggc aagggtgctg accgggaatc gttgcgtgta
    28501 cttaaccgtg atctgcgttc agcgtttaac gccctgcaac aggacaagcc tctgaacgac
    28561 cgccagaaag cggcaatgcg cgagctgcca tcgtgggcgg agcttattga ggctattcgt
    28621 gaaggcgtgg aatggtacaa caaccgcccg cattctgagc tgccgatgaa accgaacgga
    28681 cggcattaca gcccgacaga gttcagaaaa aaacgccagg cagaagaaga caccgaaatt
    28741 gagtggctga gtgatcttga gctgcgcgac atgttccgcc caatggtgga acgaccggta
    28801 agacgctgcg aaattcagtg gctgaacaat atttactacg cgccggagtt gcgtgacgaa
    28861 cacggtcgca aagtgcttat cagttatgac at
//
LOCUS       DAJTYL010000043        28753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYL010000043 DAJTYL010000000
VERSION     DAJTYL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.043"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..747)
                     /gene="otsB"
                     /locus_tag="OO572_001836"
     CDS             complement(<1..747)
                     /gene="otsB"
                     /locus_tag="OO572_001836"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6660867.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQ"
     gene            complement(914..1900)
                     /gene="araH"
                     /locus_tag="OO572_001837"
     CDS             complement(914..1900)
                     /gene="araH"
                     /locus_tag="OO572_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6660868.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(1915..3429)
                     /gene="araG"
                     /locus_tag="OO572_001838"
     CDS             complement(1915..3429)
                     /gene="araG"
                     /locus_tag="OO572_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6660869.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3499..4488)
                     /gene="araF"
                     /locus_tag="OO572_001839"
                     /pseudo
     CDS             complement(3499..4488)
                     /gene="araF"
                     /locus_tag="OO572_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5285..5788
                     /locus_tag="OO572_001840"
     CDS             5285..5788
                     /locus_tag="OO572_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6660870.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(5868..6119)
                     /locus_tag="OO572_001841"
     CDS             complement(5868..6119)
                     /locus_tag="OO572_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6660871.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6234..6320)
                     /gene="azuC"
                     /locus_tag="OO572_001842"
     CDS             complement(6234..6320)
                     /gene="azuC"
                     /locus_tag="OO572_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6660872.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6583..6906
                     /locus_tag="OO572_001843"
     CDS             6583..6906
                     /locus_tag="OO572_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6660873.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7077..7574
                     /gene="ftnA"
                     /locus_tag="OO572_001844"
     CDS             7077..7574
                     /gene="ftnA"
                     /locus_tag="OO572_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6660874.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(7611..7850)
                     /locus_tag="OO572_001845"
     CDS             complement(7611..7850)
                     /locus_tag="OO572_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6660875.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8040..9251
                     /gene="tyrP"
                     /locus_tag="OO572_001846"
     CDS             8040..9251
                     /gene="tyrP"
                     /locus_tag="OO572_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6660876.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9313..9978)
                     /gene="yecA"
                     /locus_tag="OO572_001847"
     CDS             complement(9313..9978)
                     /gene="yecA"
                     /locus_tag="OO572_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6660877.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10174..10260)
                     /locus_tag="OO572_001848"
     tRNA            complement(10174..10260)
                     /locus_tag="OO572_001848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10224..10226),aa:Leu,seq:taa)
     gene            complement(10273..10346)
                     /locus_tag="OO572_001849"
     tRNA            complement(10273..10346)
                     /locus_tag="OO572_001849"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10312..10314),aa:Cys,seq:gca)
     gene            complement(10401..10476)
                     /locus_tag="OO572_001850"
     tRNA            complement(10401..10476)
                     /locus_tag="OO572_001850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10441..10443),aa:Gly,seq:gcc)
     gene            complement(10628..11176)
                     /gene="pgsA"
                     /locus_tag="OO572_001851"
     CDS             complement(10628..11176)
                     /gene="pgsA"
                     /locus_tag="OO572_001851"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6660878.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11233..13065)
                     /gene="uvrC"
                     /locus_tag="OO572_001852"
     CDS             complement(11233..13065)
                     /gene="uvrC"
                     /locus_tag="OO572_001852"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6660879.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13062..13718)
                     /gene="uvrY"
                     /locus_tag="OO572_001853"
     CDS             complement(13062..13718)
                     /gene="uvrY"
                     /locus_tag="OO572_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6660880.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14014..14190
                     /locus_tag="OO572_001854"
     CDS             14014..14190
                     /locus_tag="OO572_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660881.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14177..14401
                     /locus_tag="OO572_001855"
     CDS             14177..14401
                     /locus_tag="OO572_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6660882.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14434..15054)
                     /gene="sdiA"
                     /locus_tag="OO572_001856"
                     /pseudo
     CDS             complement(14434..15054)
                     /gene="sdiA"
                     /locus_tag="OO572_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15284..16036)
                     /gene="yecC"
                     /locus_tag="OO572_001857"
     CDS             complement(15284..16036)
                     /gene="yecC"
                     /locus_tag="OO572_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6660883.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16033..16701)
                     /gene="tcyL"
                     /locus_tag="OO572_001858"
     CDS             complement(16033..16701)
                     /gene="tcyL"
                     /locus_tag="OO572_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6660884.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(16716..17702)
                     /gene="dcyD"
                     /locus_tag="OO572_001859"
     CDS             complement(16716..17702)
                     /gene="dcyD"
                     /locus_tag="OO572_001859"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6660885.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(17807..18607)
                     /gene="tcyJ"
                     /locus_tag="OO572_001860"
     CDS             complement(17807..18607)
                     /gene="tcyJ"
                     /locus_tag="OO572_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6660886.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(18695..19246)
                     /gene="fliZ"
                     /locus_tag="OO572_001861"
     CDS             complement(18695..19246)
                     /gene="fliZ"
                     /locus_tag="OO572_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6660887.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19292..20011)
                     /gene="fliA"
                     /locus_tag="OO572_001862"
                     /pseudo
     CDS             complement(19292..20011)
                     /gene="fliA"
                     /locus_tag="OO572_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20176..21828)
                     /gene="fliC"
                     /locus_tag="OO572_001863"
     CDS             complement(20176..21828)
                     /gene="fliC"
                     /locus_tag="OO572_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6660888.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22094..23500
                     /gene="fliD"
                     /locus_tag="OO572_001864"
     CDS             22094..23500
                     /gene="fliD"
                     /locus_tag="OO572_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6660889.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23525..23935
                     /gene="fliS"
                     /locus_tag="OO572_001865"
     CDS             23525..23935
                     /gene="fliS"
                     /locus_tag="OO572_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6660890.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            23935..24300
                     /gene="fliT"
                     /locus_tag="OO572_001866"
     CDS             23935..24300
                     /gene="fliT"
                     /locus_tag="OO572_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6660891.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24378..25865
                     /gene="amyA"
                     /locus_tag="OO572_001867"
     CDS             24378..25865
                     /gene="amyA"
                     /locus_tag="OO572_001867"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6660892.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(25899..26312)
                     /locus_tag="OO572_001868"
     CDS             complement(25899..26312)
                     /locus_tag="OO572_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6660893.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26499..27704
                     /gene="yedE"
                     /locus_tag="OO572_001869"
     CDS             26499..27704
                     /gene="yedE"
                     /locus_tag="OO572_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6660894.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            27701..27934
                     /gene="yedF"
                     /locus_tag="OO572_001870"
     CDS             27701..27934
                     /gene="yedF"
                     /locus_tag="OO572_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6660895.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28041..28711
                     /locus_tag="OO572_001871"
                     /pseudo
     CDS             28041..28711
                     /locus_tag="OO572_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7171 a         7409 c         6843 g         7330 t
ORIGIN      
        1 ttgttgtaat gcggcggtta tcatttcaag ccagctccag acatccggca cacccgccag
       61 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
      121 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
      181 gataaaagga gcttcctgca taaaggctgc aatcgcttca cctttactgg tacctctcgg
      241 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
      301 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
      361 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ccgtatgcag
      421 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
      481 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
      541 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
      601 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
      661 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
      721 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtgtt
      781 gcgcagaccg cgctttacag acatgtaagt atagacagtg tgacggaggt cgccatttgc
      841 aacgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaagttgtt
      901 gtggaaaaaa gcatcagaca gtgcgcttcg ctttttgctt gtaacggtcg aagatcaccg
      961 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     1021 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     1081 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     1141 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     1201 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     1261 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtattacga ccaaaggtgg
     1321 ttttattcag cagcaaacca aagataatca gacacgcgac ggtgagccag attggcgcag
     1381 gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg
     1441 ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgtcg
     1501 tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa
     1561 cgcccagcaa caaacccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca
     1621 ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg
     1681 aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca
     1741 accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga
     1801 gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg
     1861 acttaggtgc gccagacccc gatgtagaaa cagaagacat catactctcc ttactcaggc
     1921 aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc
     1981 gtgtaacaat tcaccggcga tttcgccttc ccgcatcacc acaatccggt cggcaacgcc
     2041 gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acgccctgcg ccgccagcgc
     2101 ataaattacg ttatatattt cgtgcttagc gccaacatca atgccgcgcg taggttcatc
     2161 cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt
     2221 tccgcctgag agattcatga tcagttgctc cgcgcccggc gttttgatgt tgagcgaacg
     2281 aatgtgctga tcggcattgt tttcttccca actgttgttg attacacaac cgccgagcac
     2341 atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc
     2401 ttccgctttg cgagcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt
     2461 acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc
     2521 aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat
     2581 ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctagtgctt tcacagcatc
     2641 aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc
     2701 gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt
     2761 tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc
     2821 catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg
     2881 gaaaagattg ccgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat
     2941 tttagcgtta cgcgccagcg ctttggcgat ttcaaccatc tgccactgac caatggcgag
     3001 atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc
     3061 ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata
     3121 gatgttttcc gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac
     3181 acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac
     3241 agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc
     3301 gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt
     3361 cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga
     3421 ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag
     3481 gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa
     3541 gttgtcacgc gtgatcagta ccacgtctgt aacttcggta aattttggcg tttcaacgtc
     3601 ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg
     3661 cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctta ctgcatccac
     3721 accgttaata ccaataccga tgatatcggc tgctttaaag ccctgacctt cggttgcacg
     3781 tacgccgcca agtacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg
     3841 atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt
     3901 aggtacctga taaatttgtt tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc
     3961 cgttgtacgg cggcgggcgg tatccagttc gttggcggta atcgccatca ctgcgctttc
     4021 tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctagccct gacgttcgcc
     4081 aattttagtc gccgccatca tcaccagagg aacggtatcc attggcttac ctttggcgtt
     4141 aacaaactgg tcatccactg caatgacttt catatcgtag ccacgcgctt tcgcgacgat
     4201 ggcagagccg agtttcgggt ccggagtaca aataacgaaa ccttttgcgc cactggctgc
     4261 caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc
     4321 aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc
     4381 ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat
     4441 aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag
     4501 ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt cgttaaatta atcggaaaac
     4561 aaagcattac cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat
     4621 taattctcca taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt
     4681 tagcgggaaa agacataagg aaaagctaat ttgtcagaca aattgttgaa tgcacagcag
     4741 attaatccat aagattagcc tggaaatcct tgttgtcttc ggtacccatg cgggatgtct
     4801 tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata
     4861 accacaactc aaagatgtgg aaaatgcgcg tcattcattt cgccattaat tatcactgtg
     4921 ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt
     4981 ataaaatcat cacttcatca tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa
     5041 agcactatca ttaaaattca ttgcatttac attgatttca ccaatgaaat gtaaaaatat
     5101 ataaacttga tgatttaagc attttcttat aaccgttcag acgttattct tatttcagat
     5161 catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa
     5221 acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt
     5281 aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta
     5341 cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac
     5401 cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa
     5461 ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc caggtgaaaa
     5521 actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttcaag
     5581 cactctggcg cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt
     5641 cctgcgcgat ctggaaaaag aacagcagca tgatggcctg ttgctgcaaa ccattcttga
     5701 tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct
     5761 gaatgtcgtg tcacaccagc tgcattgatc atacatcggc gctaatacat tgcgccgatg
     5821 aagtttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
     5881 ccctgtgggg taaacttgag ttcaatcagc gcgatggctt tttggattgc ccgcatggtg
     5941 accgggtctg cggcagcggg atgatgagta aagtcgatat tcttcagctg actggacatt
     6001 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
     6061 aactggcaag ctaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
     6121 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
     6181 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
     6241 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
     6301 aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct
     6361 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
     6421 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
     6481 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
     6541 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
     6601 actctttcat taataacgct ggcggggtgt acggttactc gccaggccca tgtgagcgaa
     6661 gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
     6721 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
     6781 acccacgcga tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
     6841 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
     6901 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
     6961 tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg caaataataa
     7021 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
     7081 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
     7141 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
     7201 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
     7261 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
     7321 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
     7381 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcagt
     7441 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
     7501 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
     7561 acacacaaaa ctaatgctct ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg
     7621 caaatcttac tctgatcggt cgaaaaacct tcttctgaag gtataaattt gccttttgct
     7681 gccagaaacg ctaccagctc tcccgctgtc accccttctg ccgagcacgt gtgaaaacgt
     7741 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
     7801 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
     7861 caaaaaaagc ctagcgtagc gattaccgct tatgaagact ttgcgccagc gcaggactga
     7921 atgcttttat tgtacattta tatttacaca atatgtaacg tcggtttgac gaagcagccg
     7981 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
     8041 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
     8101 tgctggcaat gccgctggct gcggccggtg ttggttttag cgttacgtta atcttgttga
     8161 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
     8221 ttccggcaga taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
     8281 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcagca tacatcagcg
     8341 gtgccggtga attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa
     8401 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc
     8461 tggtcgattt atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc
     8521 tggtattgct gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
     8581 ggctggcact gtctgcaatc ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc
     8641 cgagtattgt cagctatatg gatggcaaca ttcgtaagct acgctgggtg tttataatcg
     8701 gtagtgccat ccccctggtg gcatatattt tctggcaggt ggcgacgctt ggcagcattg
     8761 attcaacaac ctttatggga ttgctggcta atcatgctgg attaaacggg ctgttacagg
     8821 cgttacgcga aatggtggcc tctccgcatg ttgagctggc ggtgcattta tttgctgatt
     8881 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
     8941 tgtttcagcg ttcaaatacc gttggtggac gattgcaaac tggtgcaatt acctttctgc
     9001 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
     9061 gtgtggcgct ggcggtactg gcattgatta tcccttcact attgacctgg caaagcagaa
     9121 agcacaatcc tcaggcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
     9181 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
     9241 aagtggggtg atcagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
     9301 caatgtgacc tcttaatgca ggcagcactg cttaaatttc ttaccactgc cgcacggaca
     9361 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg
     9421 cggatgcgcc atccagtatg catgtaaatc aagcgccgcc agtcgaatgg catctacgct
     9481 ctcttcaaac gcttctggcg acattttctc tacccgctcg aagttttcct cagtaccgtg
     9541 cagcgcaatc gcctccagcg ctggttttaa agaatcgggc aacgttgacc agtcagaaag
     9601 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
     9661 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
     9721 ggtatcggcc atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc
     9781 ccaacgcggc acatagtcag ccccacccca cacggcaacc agccactgtt ctggttcaat
     9841 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
     9901 ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
     9961 taacggtccc gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta
    10021 catgccggtc cgtatcagag atactttttg agtggctttg ccggtgatta aaaattaagg
    10081 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg
    10141 cttattactt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
    10201 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
    10261 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
    10321 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt
    10381 gacgccgatg tcttttaaac tggagcggga aacgagactc gaactcgaga cctcgacctt
    10441 ggcaaggtcg tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca
    10501 cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg atgcgttgca ttctacttac
    10561 ctggcgcgat gagtcaacga tatttttcac cacttttgat cgtttgctga aaattacgcc
    10621 gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc
    10681 acagagtcag taccgcagcc acaaagaaaa gtgcaatacc gacgtactca acccaaatgt
    10741 tcggacgcca cagcagccag gccaacgcca ccatctgggc agtggttttc actttcccaa
    10801 tccaggagac ggccacgcta ctgcgtttac ccaactccgc catccattcg cgtagcgcag
    10861 aaataataat ttcacgggcg atcatcgttg ccgccggtaa tgtcacccac cagctgtgat
    10921 aatgctcggt taccagcacc atggcgatag ccacgagaac tttatctgcc acagggtcaa
    10981 ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt
    11041 cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca
    11101 gataaaagac caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag
    11161 ggatattaaa ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt
    11221 gctacagaga catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat
    11281 acccggcact tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat
    11341 atatttcaac aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact
    11401 ggtatttttg accttcgccc gttttttacg gtgcccgcca atcgcgtgat catgtgattc
    11461 atcgcgaata tgctggataa catgcagcgc gggtgaatct ggcggcaaac taaatccctc
    11521 accttccggc tcaaagaaca gcgtttccag tccagcctta cgatctgctc ctttggcaac
    11581 gccaagtagc agcggatgat ttttatccca tgagacatcc agttcggcga agacattttt
    11641 cgcctgcgca agctggcctt tgccgccgtc gataaggatc acatccggga tcttactgtc
    11701 gtcaatggct ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc
    11761 cggcgtgatg cctgtaatgt tatagcgccg atactccgca cgcagcgggc cgttagcatc
    11821 aaacaccaca caggaagcga cggtttgttc gcccatggta tggctgatgt caaagcactc
    11881 catccgcttc acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac
    11941 ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt
    12001 cagataacgc gccctatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag
    12061 ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc
    12121 acccggtaag gtgcgcatct ggctgccttg taaatagaac tgacctacga aggtttctac
    12181 cacctcgctc agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac
    12241 tttgccctga cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc
    12301 aataacgtca aggtcgtcgc cggtattgga aacaaactgt ttttcggtga cgcgtcgcac
    12361 cgcctgaatt tggtcgcgaa tacatgccgc ttcttcaaac tccagattct ggctggcagt
    12421 ttccatacgg ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg
    12481 cacatactcg acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc
    12541 cagacagcgc cctatctggt attgcagaca cggacgcgag cgattgcgat aaacactatt
    12601 ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc agtgtttcac gtacggcata
    12661 gccattcggg aacgggccga aatattcacc tttggcatgc tttgcaccac gatgcatcgc
    12721 cagacgcggg tgggtatcgc cactcaggaa gataaaagga taagatttat catcgcgtag
    12781 caaaacgttg taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc
    12841 ggtttctgta tgcgtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt
    12901 tttgcgcgaa gcgaggttgc tgcggaaata gctggaaagc cgttttttca ggtctttcgc
    12961 tttgccgaca tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggct
    13021 ggttacggtt tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc
    13081 tccgcattac acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg
    13141 tttagtttac tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc
    13201 tgttctgaga tctcattgac cttctggccc ttagtgatca tcagcataat ctgcaattca
    13261 cgttcagaca aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc
    13321 atttgttgag cgatatcaga agcaatgtaa cgctgccctg aatagacaga acgaatcgca
    13381 ctcacgactt cctgcggagc cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg
    13441 actttcgctg gtaaagggtt ttctgtatgg accgtaagca tgatgatttt aacatcagct
    13501 gtggaacgcg cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc
    13561 attagcacca cgtcaacggc atttgtccgg caccacttaa cggcgtcttc accgcacgat
    13621 gcctctccga cgacttttat acccttaata tcttccagaa tgcgtcgtat ccctgcgcgc
    13681 accagttcgt ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg
    13741 ataacgctac tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg
    13801 ttaaaaatgg tgggtattcg atttttctct cgtttttgtc aattcgattg tccacagtta
    13861 tggtcacgcc cgtcaccaac ggcttaccgg atcgtttcac agtaagccct acgatgaaat
    13921 gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt
    13981 aattaacccg aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat
    14041 attaacgtat tttatagcac tgccgatatc acgctcaaaa aacaaccact gctattttta
    14101 gcaaagctta tggtatactc cgccgcctta acatttttca ccacaaattt tcattgcaac
    14161 atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact
    14221 ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg cgatgggaca
    14281 agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga
    14341 aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta
    14401 atataaaacc ggctgagagc gtgcctttca gccggttatt gacaaaatca tcgctatctc
    14461 tgctgatgtc ttcccttccg ccgtccattt cagaatttct ttttcgcgct tgctgaaatt
    14521 catctctggc gtcatcacta tttcatcatt taaacgcatc agagccatca gactttcgcg
    14581 caccagtaac tgcattttta attgcagttc atcactaaga atgggtattt cgcgcgtgct
    14641 gcaacgggaa aaggacaaaa agcccagcgc ccgatttggc agcattaaat actgagtgac
    14701 accgcggcgt aaaccatgtg cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa
    14761 gtcatcattc cacattaaat ggccctgact aaagttttca gggttcagca caggatcaat
    14821 tgcgagaaaa ttttttgcct gataataact aacccacgac tcagggtaat tggtgtagaa
    14881 agccacttta ggtcgcgtga atggtaccgg gtggcgaaca cataacgaat agtaatcgta
    14941 ctccagctgc tgagcctgaa gctctatttc atggtaggcc tcttctgcgg cctccatcct
    15001 ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa
    15061 ccgcaacgcc cctgagagtg agtatcattt ataatgataa ttcaaactat cacataaata
    15121 agatttatat ataatttata ttattcaggc aatgaattac ttttgcaagc catcgcattc
    15181 tcttatgtta ttaatgagtt atgctgattt gttaagcagt tttatcacgc ttgaaatggc
    15241 gtcccgcccc gacaggtgaa tcgtcggggc tgattttttc ttattattgc agcagaagct
    15301 tctcgaggaa ctggcgggtg cgaggctgct ggggatcggc aaataacgct tttgctgccc
    15361 cctgctcgac tatccgcccc tggtccataa agatcgcccg gtccgcaaca tcccgggcaa
    15421 agctcatttc gtgtgtcaca atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg
    15481 tgttcaggac ttcgcccacc agctctggat ccagcgccga cgttggctcg tcaaacaaaa
    15541 tcacctcagg acgcattgcc agtgcacgcg caatcgcaac acgctgctgt tgaccgccag
    15601 acaaacgacg tggatagctg gtttctttac cggccagccc aactttcgcc agcagctcgc
    15661 gagcgcgcgc cgtggcctct tctttcggtt cgcctttgac gatcaccggc ccttcaataa
    15721 tgttttccag caccgtacga tgcggaaaca aattaaagtt ctggaagaca aacccgacgt
    15781 gctgacgcaa ctggcgaatc agcgattttt gctgacttaa tgaacgtgca gtatcaatag
    15841 tgatatcgcc aacggtgatc gttcccgctt ccggttgttc cagcagattt atgctacgta
    15901 gcaatgtggt tttgccggaa ccactcggac cgataattgc caccacttcg ccaggcttta
    15961 cctcgaggtc gataccgtgc agcaccgtct gaccgtggaa ttttttcacc aggttcttaa
    16021 cttcaatggc actcattttg gttctctctc ctggcgatta agttgattct caaaatggtt
    16081 ctgcaacgtc gataacacag tcgccatgat ccagtagatg agcgaagccg ccaggtacat
    16141 ggtgaacacc tccagcgtgc gcgaggtaat caactgcgcc tgacggaaca gctccggcac
    16201 ctggattgtc gcggccagcg acgtatcttt taccagactg ataaagctgt tcgacagcgg
    16261 cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt
    16321 cataccaata ctggcagcgg cttcccactg acctttatca atagaagaaa tggcggcgcg
    16381 cagcgtttcg gcggcatagg cggcagtgtt cagcgacaga ccaatcattg ctgacggaat
    16441 gggatctaat tcgataccaa actgcggcag gccgtaatag atcataaaca gttgtgcgat
    16501 gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc agccagcgca cgggccagat
    16561 tggcgacagg cgcatcagcg cgagaataaa cccgagtagt aagccaaaaa acatgccgcc
    16621 gatactgagt tgcagggtat acccagcccc tttcaacagg aacggcaagg aatcaataac
    16681 cagttgtata ctttcttgca tgagcgttcc cggagctaaa cgtggggatg ataggcgaac
    16741 agcgcaggcg cgccgccggt atgaataaac agaatcggcc cttcatcttt gaagcgtttc
    16801 tgactgatac cgtcaatcag ccccgccatc gcttttccgg tatacacagg atcaagcaga
    16861 atgccttcca gacgcgccag caatttcact gcttccatgc cttcgtcgtt cggcacgccg
    16921 tagccaggtg caaaatagtc atcccagagt aaaatttccg ctgatgcggt cagctccagt
    16981 tctttcgcaa tcgcctgttg taggttaacc actttcggca attgatcggc aacggaacgc
    17041 gacacggtca cgccaatcag ttcgctttca ggcatcaggt gttccagccc aacagccagt
    17101 ccggcgtgag ttccggcact gcccgatgcg actaccaccg acgaaatatt aaccgcccct
    17161 tcacactgtt gcgcgatttc cagcgcactc tccacataac ctagcgcgcc aagagcatta
    17221 gaaccgccaa ccggaatgac atacgggcga aagccttgtg cttcgactcg cgtcgccagt
    17281 tcttccagtt gggcattggg atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac
    17341 agatccagca acaaacgatt gccgttggtt aaatagtttt ctgcggttgt gccaatagga
    17401 ttttccagca gcgccacgca gtgcagaccg agtttcgccg cgactgcggc agtctggcgc
    17461 acatggttag actggatcgc gccggcagta atcagcgtat cggcaccttc gcgcagggca
    17521 tctgccgcga gaaattccag cttacgtaat ttattgccgc ccattgccat gggggtgacc
    17581 tcatcccgtt tgatgaaaat ttcccgtcca agataatcag aaaagcgcgg cagatattcg
    17641 agcggcgttg gcgcgccgat aaactccaac cgtggaaaac gggttaaatt atgcagtggc
    17701 ataacagcct ccgatgtgtg ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa
    17761 ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta tgctgattat ttggtcacat
    17821 cagcaccaaa ccatttttcg gaaagagctt gcagagtgcc gtctttttgc atttccgcaa
    17881 ttgcatcatt caccgctttc agcaggtctt catttccttt acgcagcgcc acgccagact
    17941 cctgacggga gaatgcttca ccggttactg ccagcgtatc gttggttttc ttcaccagat
    18001 ccagcgccgc cagacgatca acgaggatcg catcgatacg ccctacgcgc agatcctgat
    18061 atttggtcgg gtcatcatca taggtacgca catcgacacc ctgaacattc tgccgcagcc
    18121 actcttcata gttggtgccc agaccgacac ccactttttt gcctttcaga tcagcagccg
    18181 ttttaatggt gccttcgtta ccttttttca ccagcgcctg aataccggaa atggtgtaag
    18241 gggtagagaa atcgtatttt ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca
    18301 catcaatgcg tttagagtcc agcgacgcca gcataccgtc ccatttggtc ggttttagtg
    18361 acgcctcaac gccaagatgt tttgccagct gttgggcaaa ttccacttca aaaccggtta
    18421 atttgccgtc atccccctga aaactgaacg gcggataagt gccttccagc cctaccagca
    18481 gcgtgccgct ctctttgact ttattaagca gaccttcatc tgcaaaactt ttaacgctca
    18541 tgcccgcaac cagcgccacg gccatcacac ccatcaatgc ctgacgtccc agatgtgcta
    18601 atttcatatt caccccgaat gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa
    18661 acacaaaaac aactccgcta catcttattc ttatttaata tatatcagaa gcaggcaggc
    18721 tggaggattt ctgcacaagt ccggtacagg tttggcgctg atagtgttga tacttccgta
    18781 atgcgatgcg gtaattgttg gtgctggtgg tgggcagcca cggggcgagg atttcatcaa
    18841 ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc atgtagatga ttacccaaac
    18901 ggcgcagacg aacgacatat tcacgcaccg tacctgggct catttcagtt tgctcaaaaa
    18961 gaaatttctt aaagccgata atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac
    19021 cgcaaaatcg gcacaatgcc gcccatgatt gttgttccgt ttgccagcct ttttcatcaa
    19081 gcagcgtgtc caggcgggaa atctcgattt tattcactat tttgccgtcg cgaactaagg
    19141 taatgcgatc gagtaattta cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt
    19201 taagatagcg gcttaatggc cgtcttttca ggtgctgcac catcattaag aactcctggt
    19261 agtcaaagtt aaagtgcggc atttactgac gttataactt acccagttta gtgcgtaacc
    19321 gtttaatgtc ctggctgtgt aactgactga cccgcgattc cccgacctcc agcaccgcgc
    19381 caatctcttt gagattcagc tcttcctggt aatagagggt taataccagt ttttcgcgct
    19441 ccggcaacgt ttcgatggct tccatcaccc gctggcgcag attactgtcc agcagttgtt
    19501 gtagcgggtt ttctcgctga tgatcatcag taaccagttc gatgctatcg ccgtgctctt
    19561 cacgccactc atcgtaggag aagagctggc tgttattggt gtcgagcagc atttgacgat
    19621 aatcggcaat atcgatccct aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa
    19681 gttcctgctc cagttgccct attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc
    19741 gcggcaccca gtcacggctg cgaagttcat ccagcatagc gccacggata cgctacactg
    19801 cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg ttcgacagca ttaagtaacc
    19861 caatgccgcc cgcctgtagc agatcgtcaa gttccacgct cgcgggcagt cgaacctgca
    19921 ggcgcaatgc ttcgtgacgc accagcggga cataacgctg ccacagcgag tgtttatcca
    19981 ttacaccttc agcggtatag agtgaattca cgataaacag ccctgcgtta tatgagttat
    20041 cggcatgatt atccgtttct gcagggtttt taatcggacg attagtgggt gaaatgaggg
    20101 gttatttggg ggttacgcaa aaagccgaat gtaaaaaacc ccgccggtgg cggggtttga
    20161 gaggcattca gacgattaac cctgcagcag agacagaacc tgctgcggta cctggttggc
    20221 ttttgccagc acggagttac cggcctgctg gataatctgc gctttcgaca tgttggacac
    20281 ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct tcagacaggt tggtagtggt
    20341 gttgttcagg ttggtgactg cggaatccaa acggttttgc accgcaccca gggaggagcg
    20401 gaatttgtcg atggagctga tggcttcatc cagagctttc aggggatcgg ctgtcgtagc
    20461 tgcttttgtt tcagcatctg tcgtcaattt accatcagca tctttataaa ctgccttacc
    20521 gctgccgtta gtcactgaac catcatcctg taggtaaaaa tctacagtat cagatgtggt
    20581 caacgcctcg ttgttatcaa cataaaccga atcaacacct tttgttacag cgccagttgt
    20641 gccgttgacg ctataacctg cagtagtgaa atcaacattg tttcccgagg ttgatacagc
    20701 tttatcgaaa gctgcctgat caacttttgc acctgttact gaataagtaa tagtgtccgg
    20761 cgtagtacta ttaccggtaa aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt
    20821 ggtattattg gctgcaagtg tggacagact agcctcagtc aaagaagcac cagagccgtt
    20881 tttcgttaag cgcccagttg tatcaatgta gagtttatct ccattgcttg ccgtaatttt
    20941 accatcactg ctgatctgaa catcctgtgc agaaccatcg atagtaatag tgcccttagc
    21001 ggtatcacca actcccgggg tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt
    21061 atcaaaagag tatgacttac ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt
    21121 tccgaagccg ttattaatgg ttgcctgaac agtatcacca tcactaactc cagccagcaa
    21181 atcagacgct ttagcagcat cgtaacccgc actcacagta tatttgttgc cgaccactgt
    21241 tgcattagca gctaccaagt cagctttaga tgcagcagta ttagccacag ccccgccacc
    21301 attcacatta aacccattca gccccagcgt atcagagtca attttcttca gatcaatagt
    21361 gatagtctgg ccgtcattcg caccaacctg aattttcatc gaaccgtctt ttgccagtac
    21421 gttcacgccg ttgaactggg tctgaccgga tacgcggtca atttcgtcga gacgggattt
    21481 gatttcgtcc tgaatggagt ccagatccga atcagagtta gtcccggtag aagcctgaac
    21541 cgtcagctca cggatacgct gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt
    21601 ctgtgcaaca gaaataccgt cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt
    21661 agaagtaaaa cggttagcaa tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg
    21721 caagccagaa gacagacgct cgatagaact cgacagcgca gactggttct tgttgatatt
    21781 attttgagtg atcagcgaga ggctgttggt attaatgact tgtgccatga ttcgttatcc
    21841 tatattgcaa gtcgttgatt acgttttggg tttccacccg tcggctcaat cgccgtcaac
    21901 cctgttatcg tctgtcgtaa aacaaccttt agaatttttt tcacaaacag ccattttttg
    21961 ttagtcgacg aaatactctt ttctctgccc cttattcccg ctattaaaaa aaacaattaa
    22021 acgtaaactt tgcgcaattc aggccgataa ccccggtatt cgttttacgt gtcgaaagat
    22081 aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc gggtcaggtc tggatttaag
    22141 ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca
    22201 gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac
    22261 tttccagacc gccaatactg cattgtctaa agccgatctt ttttccgcca ccagcaccac
    22321 cagcagcacc accgcgttca gtgccaccac cgcgggtaac gccatcgccg ggaaatacac
    22381 catcagcgtc acccatctgg cgcaggcgca aaccctgacc acgcgcacca ccagagatga
    22441 tacgaaaacg gcgatcgcca ccagcgacag caaactcacc attcaacaag gcgacgacaa
    22501 agatccgatt accattgata tcagcgcggc taactcatcg ttaagcggga tccgtgatgc
    22561 catcaacaac gcaaaagcgg gcgtaagtgc gagcatcatt aacgtgggta acggtgaata
    22621 tcgtctgtca gtcacatcaa atgacaccgg ccttgataat gcgatgacac tctcggtcag
    22681 cggtgatgat gcgctacaaa gttttatggg ctatgacgcc agtgccagca gcaacggtat
    22741 ggaggtctcg gtcgccgccc agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa
    22801 cagcagcaac actatcagcg acgcgctgga aaacatcact ctgaacctga acgatgtcac
    22861 cacgggcaac cagacgctaa ccatcactca ggacacctcc aaagtgcaaa cggcgattaa
    22921 agactgggtg aatgcctata actcgctaat agataccttc agcagcctga ccaaatacac
    22981 cgccgtagat gcgggagctg atagccagag ttctagcaat ggcgcactgc tcggcgactc
    23041 cacgctgcgg acgattcaga cgcagttgaa gtcgatgctg agtaataccg tcagttcttc
    23101 cagctataaa acgctggcgc agattggtat cacgaccgat cccagcgatg gcaaactgga
    23161 actggatgcc gacaaactca ccgctgcact gaaaaaagat gccagcggcg taggtgcatt
    23221 gattgttggc gatggtaaaa aaaccggcat cacaaccacc atcggcagca acctgaccag
    23281 ttggctttcg acaacgggca ttattaaagc cgctaccgat ggcgttagta aaaccctgaa
    23341 taagttaact aaagactaca acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta
    23401 caaagaacaa tttacccaac tggacgtttt aatgacctcg ttaaacagca ccagcagcta
    23461 cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa tcattattca tcgggagaca
    23521 ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa attggcgtcg aaagcaccgt
    23581 aatgagcgcc agccaacagc agttggtcac catgctattt gatggagtgc tgagcgcact
    23641 ggttagagcg cgactgttta tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc
    23701 aaaagcgatc aacatcattg agaacggact gcgggtgagt cttgatgaag agagcaaaga
    23761 cgaattaacc caaaacttaa ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc
    23821 caatttacgc aacgatgtct cagcagtcga agaggtggaa gcattaatgc gtaatattgc
    23881 cgatgcctgg aaagagtctt tactctcccc ttcttcgatt caggacccag tctgatgaac
    23941 aatgcaccgc atttgtattt cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta
    24001 cgcctggcaa cggaagaaca atgggacgaa ctcatcgcta gcgaaatggc gtatgtgaat
    24061 gcggtgcagg agatcgcaca cctgactgaa gaggttgccc cgtccactac gatgcaggag
    24121 cagctccgcc cgatgctgca cctgattctc gacaacgaaa gcaaggtaaa gcagttatta
    24181 cagattcgga tggatgaact ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg
    24241 ttaagtgcct atggcgatca gggcggcttt gtgctggctc cgcaggataa cctcttttga
    24301 atcttaatga gccgatggct cgcgaataat ccgattacgg ctacgcttct aatattcccc
    24361 ttgaatggag tcgaagaatg cgtaatccca cgctgttaca atgttttcac tggtattacc
    24421 cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc agacggtttt aatgatattg
    24481 gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg
    24541 gctacgactc ctatgattta tttgatctag gtgagtttga tcagaaaggc agcatcccta
    24601 ctaaatatgg tgataaagca caactgctgg ccgccattga cgccctgaaa cgtaatgaca
    24661 ttgcggtgct gctggacgtg gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta
    24721 ttcgcgtgca gcgtgtaaat gctgatgacc gtacgcaaat tgacgaagaa atcattgagt
    24781 gtgaaggctg gacgcgttac accttccccg ctcgtgccgg gcaatactcg cagtttatct
    24841 gggatttcaa atgttttagc ggtatcgacc atatcgaaca ccctgatgaa gatggcattt
    24901 ttaaaattgt taacgactat accggagaag gctggaacga tcaggttgat gatgaactcg
    24961 gtaatttcga ttatctgatg ggtgagaata tcgattttcg caatcatgcc gtgacggaag
    25021 agattaaata ctgggcgcgc tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg
    25081 atgcggtcaa acatattccg gcatggtttt ataaagagtg gatcgaacac gtacaggaag
    25141 ttgcgccaaa gccgctgttt attgtggctg aatgctggtc gcatgaagtc gataaactgc
    25201 aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga
    25261 aattccatga agcatcgcgc atgggtcgcg actacgacat gacgcagatc ttcactggga
    25321 cgctggtgga agccgatcct tttcacgccg taaccctcgt tgccaaccac gacacccaac
    25381 cgttgcaagc cctcgaagca ccggtcgaac cgtggtttaa accgctggcg tatgccttaa
    25441 ttttgttgcg ggaaaatggc gttccttcgg tgttctatcc ggacctctac ggtgcacatt
    25501 acgaagatgt cggtggtgac gggcaaacct atccgataga tatgccaata atcgaacagc
    25561 ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg
    25621 accatccgaa ctgcattgcc tttagccgca gtggcaccga cgaatatccc ggctgcgtgg
    25681 tggtcatgtc gaacggagat gatggcgaaa aaaccattca tctgggagag aattacggca
    25741 ataaaacctg gcgtgatttt ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg
    25801 gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt gtgggttatc gaagaggtga
    25861 tttaaatcca tccccggcgg caagccgggg agatttcatt acggcagtgg cgtcggtaac
    25921 ggcgctttat ccagcgcggc ggcacactct gccgtcgggc gatccacacg ctccatcgtc
    25981 ataccatcgt actcaatggt gttaccgtca cgctcaacct cgtacagctc acgttttacg
    26041 gtaacgttag tgagatcatc agacatcaat gtcaacttac ccggcacggc aatcccccgc
    26101 tgccactgac ggcaatccaa ggtatcgccc tctttagtga caatcaggct gcctatcgct
    26161 tccggactga ccagcgcacg ttgcggccct ttggtctgcc agtagcccgc cagccagtcc
    26221 ggcgcggggg ttttcacgac attgttatag ttttcgactt cggcacagcc cgccagcaac
    26281 atgagcgcgc ctgcaattgc gagttttttc atcattcgtc ctgcatgaga agaaaagagg
    26341 attgtggcat taaagctctg atgtcgccag cctttccagg ttcagagaga aatttgatct
    26401 aactattatt tttgtgaatt attaccccga aaataaattt gcatggtgta tgattcgcgg
    26461 ctcttgtttc aaaccttctg agttcagagg ctacattcat gtcatggcag caattcaaac
    26521 acgcctggtt gattaaattc tgggcaccca tccctgcggt catcgcggcg ggtattctct
    26581 ccacttacta ttttggcatt actggcacct tttgggctgt cacgggtgaa tttacccgtt
    26641 ggggcggtca gctcctgcag ctgtttggcg tccatgctga agagtggggt tattttaaaa
    26701 ttatccatct ggaaggatcg ccattaaccc ggatcgacgg catgatgatc ctcggtatgt
    26761 ttggcggctg ctttgctgct gcgctgtggg ccaacaatgt caaactacgc atgccacgca
    26821 gccgtatccg cattatgcag gccatcattg gcggcattat cgccggtttt ggcgcgcgtc
    26881 tggcaatggg ctgtaacctg gcggcgttct ttaccggcat tcctcagttc tcgctgcatg
    26941 cctggttctt tgccatcgcc actgccattg gttcatggtt tggcgcgcgc tttaccctgc
    27001 tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc
    27061 aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc
    27121 tgggctgggc gctgctcacg gcgatgaacc aaccaaaact ggggctggca atgctgtttg
    27181 gcgtcggctt tggtttactg attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg
    27241 atatgtggat caccggacgt acccatatgg cgaaagcaat cattatcggt atggcggtga
    27301 gtgccatcgg gatcttcagt tacgtacagt taggcgttga acctaaaatc atgtgggcgg
    27361 gaccaaatgc ggtaattggt ggtttacttt ttggttttgg catcgtgctg gcaggcggct
    27421 gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt gcactactgg tgggtcggtc
    27481 tgggcaacgt gatcggctca acgattctgg cgtattactg ggatgatttc gctccggcgc
    27541 tggccaccga ctgggacaaa atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc
    27601 tggtgacata tttgctgctg tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct
    27661 tcttccgccg tgaggcaccg cagactgcta aggagatcgc atgaaaaata tcgttcctga
    27721 ttaccgcctt gatatggtgg gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc
    27781 gatgccgcaa ttaaaaaaag gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat
    27841 caataatatt ccactggatg cgcgtaatca cggctacacg gtgctggata ttcagcaaga
    27901 cgggccgacc attcgttatt taattcagaa gtaatccctc attcccgctg tggcccacag
    27961 cgggaacatc gttacctctg acacatagat tttacccgcc ccctctccta ttcttttcag
    28021 tatgatctca ggaggttgct atgtgtggac gctttgccca atcccaaacg cgtgaggatt
    28081 accttgcgct tctcgcggaa gatattgaac gcgatattcc ctacgatccc gaacccattg
    28141 gcagatacaa tgtcgcgccg ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc
    28201 ttcatctgga tccggttttc tggggatatg ctcccggatg gtgggataaa ccgccgctga
    28261 ttaacgcccg cgtagaaact gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg
    28321 gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa
    28381 gccttatttt atctatcgcg ctgacggaca acctgttttt atggccgcga taggtagcat
    28441 accgtttgag cgtggtgacg aagccgaagg atttttgatc gtcactgcgg cggcagatca
    28501 aggtctggta gatattcatg accgccgccc gctggttctg tcgccagaag ctgcacggga
    28561 atggatgcgg caagacattg gtggtaaaga agcctcagaa atcgctacca gaagctgtgt
    28621 gccagcaaac cagttcacct ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca
    28681 gggcgcggag ttaattcaac ctgtttgttg acatcctcca cgccctgaat gacgaggacc
    28741 cctgctacgt tca
//
LOCUS       DAJTYL010000044        28584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYL010000044 DAJTYL010000000
VERSION     DAJTYL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.044"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1705
                     /gene="aspS"
                     /locus_tag="OO572_001872"
     CDS             <1..1705
                     /gene="aspS"
                     /locus_tag="OO572_001872"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6660896.1"
                     /translation="VNRRRDLGSLIFIDMRDREGIVQVFFDPDRADALKLASELRNEF
                     CIQVTGTVRARDEKNINRDMATGEIEVLASSLTIINRADVLPLDSNHVNTEEARLKYR
                     YLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDIETPMLTKATPEGARDYLVPSRVH
                     KGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTA
                     PQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLL
                     KSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLAYIKVNER
                     AKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMIFFGADNKKIVADGMGALLLKVGK
                     DLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHPFTSPKDMTAAELKAAPENAVANA
                     YDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEEEQREKFGFLLDALKYGTPPHAGL
                     AFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPTALAELSIQVVKKAEN
                     N"
     gene            1823..2275
                     /gene="nudB"
                     /locus_tag="OO572_001873"
     CDS             1823..2275
                     /gene="nudB"
                     /locus_tag="OO572_001873"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6660897.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2304..3044
                     /locus_tag="OO572_001874"
     CDS             2304..3044
                     /locus_tag="OO572_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6660898.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3079..3600
                     /gene="ruvC"
                     /locus_tag="OO572_001875"
     CDS             3079..3600
                     /gene="ruvC"
                     /locus_tag="OO572_001875"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6660899.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(3602..4204)
                     /locus_tag="OO572_001876"
     CDS             complement(3602..4204)
                     /locus_tag="OO572_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6660900.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4274..4339
                     /gene="yobI"
                     /locus_tag="OO572_001877"
     CDS             4274..4339
                     /gene="yobI"
                     /locus_tag="OO572_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6660901.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            4478..5089
                     /gene="ruvA"
                     /locus_tag="OO572_001878"
     CDS             4478..5089
                     /gene="ruvA"
                     /locus_tag="OO572_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6660902.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5098..6108
                     /gene="ruvB"
                     /locus_tag="OO572_001879"
     CDS             5098..6108
                     /gene="ruvB"
                     /locus_tag="OO572_001879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6660903.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6255..7040)
                     /gene="znuB"
                     /locus_tag="OO572_001880"
     CDS             complement(6255..7040)
                     /gene="znuB"
                     /locus_tag="OO572_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6660904.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7037..7792)
                     /gene="znuC"
                     /locus_tag="OO572_001881"
     CDS             complement(7037..7792)
                     /gene="znuC"
                     /locus_tag="OO572_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6660905.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            7871..8803
                     /gene="znuA"
                     /locus_tag="OO572_001882"
     CDS             7871..8803
                     /gene="znuA"
                     /locus_tag="OO572_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6660906.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            8819..10141
                     /gene="mepM"
                     /locus_tag="OO572_001883"
     CDS             8819..10141
                     /gene="mepM"
                     /locus_tag="OO572_001883"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6660907.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10261..11232
                     /gene="lpxM"
                     /locus_tag="OO572_001884"
                     /gene_synonym="msbB"
     CDS             10261..11232
                     /gene="lpxM"
                     /locus_tag="OO572_001884"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6660908.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11363..12805)
                     /gene="pyk"
                     /locus_tag="OO572_001885"
     CDS             complement(11363..12805)
                     /gene="pyk"
                     /locus_tag="OO572_001885"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6660909.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(12933..13802)
                     /gene="hexR"
                     /locus_tag="OO572_001886"
                     /gene_synonym="yebK"
     CDS             complement(12933..13802)
                     /gene="hexR"
                     /locus_tag="OO572_001886"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6660910.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14139..15614
                     /gene="zwf"
                     /locus_tag="OO572_001887"
     CDS             14139..15614
                     /gene="zwf"
                     /locus_tag="OO572_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6660911.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15849..17660
                     /gene="edd"
                     /locus_tag="OO572_001888"
     CDS             15849..17660
                     /gene="edd"
                     /locus_tag="OO572_001888"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6660912.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            17697..18338
                     /gene="kdgA"
                     /locus_tag="OO572_001889"
                     /gene_synonym="eda"
     CDS             17697..18338
                     /gene="kdgA"
                     /locus_tag="OO572_001889"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6660913.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18394..19572)
                     /gene="purT"
                     /locus_tag="OO572_001890"
     CDS             complement(18394..19572)
                     /gene="purT"
                     /locus_tag="OO572_001890"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6660914.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            19706..19996
                     /gene="yebG"
                     /locus_tag="OO572_001891"
                     /pseudo
     CDS             19706..19996
                     /gene="yebG"
                     /locus_tag="OO572_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20063..20419
                     /locus_tag="OO572_001892"
     CDS             20063..20419
                     /locus_tag="OO572_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6660915.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            20746..21405
                     /locus_tag="OO572_001893"
     CDS             20746..21405
                     /locus_tag="OO572_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6660916.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            21615..23675
                     /gene="ptrB"
                     /locus_tag="OO572_001894"
     CDS             21615..23675
                     /gene="ptrB"
                     /locus_tag="OO572_001894"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6660917.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(23672..24334)
                     /gene="exoX"
                     /locus_tag="OO572_001895"
     CDS             complement(23672..24334)
                     /gene="exoX"
                     /locus_tag="OO572_001895"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6660918.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24358..25014)
                     /locus_tag="OO572_001896"
                     /pseudo
     CDS             complement(24358..25014)
                     /locus_tag="OO572_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25116..25346)
                     /gene="holE"
                     /locus_tag="OO572_001897"
     CDS             complement(25116..25346)
                     /gene="holE"
                     /locus_tag="OO572_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6660919.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            25485..25859
                     /gene="yobA"
                     /locus_tag="OO572_001898"
     CDS             25485..25859
                     /gene="yobA"
                     /locus_tag="OO572_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6660920.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            25863..26735
                     /gene="copD"
                     /locus_tag="OO572_001899"
                     /pseudo
     CDS             25863..26735
                     /gene="copD"
                     /locus_tag="OO572_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            26748..27089
                     /locus_tag="OO572_001900"
     CDS             26748..27089
                     /locus_tag="OO572_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6660921.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(27482..28559)
                     /locus_tag="OO572_001901"
                     /pseudo
     CDS             complement(27482..28559)
                     /locus_tag="OO572_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
BASE COUNT         7227 a         7018 c         7352 g         6987 t
ORIGIN      
        1 ggtcaaccgt cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg
       61 tatcgtgcag gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact
      121 gcgtaatgag ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat
      181 taaccgcgat atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa
      241 ccgcgcagat gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa
      301 ataccgctac ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa
      361 aatcaccagc ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc
      421 gatgctgacc aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca
      481 caaaggtaaa ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat
      541 gtccggtttt gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc
      601 tgatcgtcag cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca
      661 agtgcgtgaa gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga
      721 tctgggcgat ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa
      781 accggatctg cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga
      841 gtttgcagta tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt
      901 tccgggtggc gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat
      961 ctacggcgcg aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg
     1021 tatcaacagc ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg
     1081 tactgccgcg caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc
     1141 cgacgggatg ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag
     1201 caaatgggca ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg
     1261 cctgacggca atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa
     1321 agctgcaccg gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt
     1381 gggcggtggt tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct
     1441 gggtatcaac gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata
     1501 cggtactccg ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac
     1561 tggcaccgac aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct
     1621 gatgactgaa gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt
     1681 tgtgaagaag gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat
     1741 caaggcggca actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga
     1801 aggcagtcaa cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt
     1861 agtggtcatc tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc
     1921 cgatttctgg cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc
     1981 catgcgcgaa gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat
     2041 tgactgtcag cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc
     2101 gggcgtgacg cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat
     2161 cgttttcact gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac
     2221 taagtcctgg agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg
     2281 cagggctatc tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc
     2341 gtaaagctgc gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg
     2401 taaccgcggc taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg
     2461 tggataaagc actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg
     2521 gtgtgggcgg tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg
     2581 gcggcacggc aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag
     2641 tgcgtcacgc attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc
     2701 tgttcagcaa aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag
     2761 cagctctgga agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct
     2821 acactgcatg ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag
     2881 cagacagcgc ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg
     2941 cgccgaaact gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt
     3001 accataacgg tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca
     3061 gcaaaacgga gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc
     3121 ggctacggcg tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata
     3181 cgcaccaaag tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa
     3241 atcatcaccc agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac
     3301 gctgactcag ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat
     3361 caggaattgc cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg
     3421 ggtagtgccg aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct
     3481 aatccacagg cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt
     3541 cagaatgcga tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa
     3601 gttataccgc ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca
     3661 ccaaacccgg atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag
     3721 aacctaagaa ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt
     3781 gtccaaacgt ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga
     3841 caaattttga acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc
     3901 gtagccggga gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt
     3961 ctaatcgctt tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg
     4021 tgatggttga aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga
     4081 taatgatccc gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg
     4141 cagcacctat acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat
     4201 tcacgttgtc attccattga aatagataca aatatacgta atagcataaa aaaattattt
     4261 ttcaggagtc gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa
     4321 atatttgctt ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg
     4381 ggacacctcg caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat
     4441 acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat
     4501 catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt
     4561 gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt
     4621 cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga
     4681 acgcacgttg ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc
     4741 gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg
     4801 ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat
     4861 gaaagaccga tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact
     4921 cacgtctcct gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt
     4981 ggcgctaggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga
     5041 caccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg
     5101 attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc
     5161 gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg
     5221 gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt
     5281 ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt
     5341 aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc
     5401 actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt
     5461 gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa
     5521 ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc
     5581 acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg
     5641 gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg
     5701 cttgagatga gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt
     5761 attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc
     5821 atctcggcag atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc
     5881 gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta
     5941 ggtctggata acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg
     6001 gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg
     6061 acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc
     6121 gctcgcgccg catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag
     6181 gccagccaga gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg
     6241 tgttcagcga tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc
     6301 gcacatagca ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg
     6361 ccagttactg ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg
     6421 gcaaagcgac gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg
     6481 acaaatttca tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact
     6541 tgctgtaatt tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa
     6601 ttgcgccatt gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg
     6661 agatcttctg gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga
     6721 atgttagaca tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc
     6781 cctaataacg tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc
     6841 agcagcagcg taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac
     6901 gcgacaccaa gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag
     6961 actacaaacg aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg
     7021 ggaaataata attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta
     7081 aatcgtgacg atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac
     7141 caaacattga aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa
     7201 tgtggtgatt caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag
     7261 aaaccattaa aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg
     7321 ccacttggcc atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc
     7381 aatttaacaa tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca
     7441 tcggtgcgtt aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt
     7501 tatgtgtacc agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga
     7561 gatacagctt ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt
     7621 catcgggtgt taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg
     7681 gcccaagtaa agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc
     7741 ggcgttggcc aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt
     7801 tagtcttgca gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat
     7861 tggtcgcatt atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg
     7921 gggaggtgca acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt
     7981 catcgcttct gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc
     8041 ttcagaacat gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt
     8101 agtcgtttgg gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc
     8161 agaagcgaag caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag
     8221 tattcacggc gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca
     8281 cggcgatttc aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc
     8341 aatccatgga aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct
     8401 gaaggatttt gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc
     8461 gctcaagggg aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt
     8521 cggactgaca ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg
     8581 tttacatgaa ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc
     8641 acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac
     8701 gttggatccc ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag
     8761 tcaattagcc aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt
     8821 gcaacagata gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt
     8881 tatgttgggg tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta
     8941 tcaccgtgat gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc
     9001 gctcttacct gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc
     9061 ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc
     9121 cactggcgat acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac
     9181 ccaacttgct gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg
     9241 gacattaacc gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac
     9301 ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg
     9361 agagtgggtt aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag
     9421 aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat
     9481 ggatttccgc aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct
     9541 tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga
     9601 ttattacgca atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc
     9661 gaaaggattc ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc
     9721 gcgtcgaact aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat
     9781 gccacaaggt acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag
     9841 tggcgcagca ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat
     9901 gcacttgcgc aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc
     9961 gctttccggt aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa
    10021 ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc
    10081 cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta
    10141 attaacatcc attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg
    10201 caagttgtgc cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc
    10261 atggaaacga aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac
    10321 ccgcgctact ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg
    10381 ccaaagttcc gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa
    10441 agctcacgcc gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct
    10501 gaacgcgaag cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg
    10561 gctgagttgg caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg
    10621 gagatcatcg aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt
    10681 tgggccgtcg atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg
    10741 ttccataatc agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt
    10801 ggcggtcgtc tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag
    10861 gggtactggg gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg
    10921 gatttctttg ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc
    10981 cgtgcgcgcg ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt
    11041 caggtgcgcc caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg
    11101 atgaatgaag aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta
    11161 aaattgctga aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat
    11221 cccatcaaat aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt
    11281 tgcttcagaa tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt
    11341 ttcgccgcat ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac
    11401 ccacggtact catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac
    11461 ctttatcgcg cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat
    11521 caaagggcac cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc
    11581 gcgacatggc gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac
    11641 ccgattcggt catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg
    11701 ccgacatggc aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa
    11761 cgttgatgct cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt
    11821 ctgacggata ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca
    11881 gaacggcgtt tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg
    11941 actccatcat ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca
    12001 acgctttctg aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg
    12061 caaccattac cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg
    12121 cttccgcacg ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc
    12181 gacgggcata gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc
    12241 caatcaacgc cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca
    12301 aaccgccgcc aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt
    12361 cggtgaacac tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca
    12421 gcagcaggat gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt
    12481 tttctttgtc gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat
    12541 tgaggaaaac tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac
    12601 ccagaatagc cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca
    12661 ttttgtgatc ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg
    12721 cgataacttt ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt
    12781 ttgttctgcg aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc
    12841 ttgaacatga aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat
    12901 gtcgtataag attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac
    12961 tgcttatcaa aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat
    13021 tttgcaccgc gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca
    13081 agtcgagaaa ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc
    13141 agcgttgctt cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg
    13201 ttttcgcgtg ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc
    13261 agcaccacca cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca
    13321 tcggagtaca ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg
    13381 gcggcagaag agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg
    13441 gcgcggttga tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg
    13501 gactcaaata ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta
    13561 acatagggag tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg
    13621 cgcgtgtcca tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc
    13681 gccagagcag caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca
    13741 actttgcgct ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc
    13801 atgatgtaaa gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag
    13861 ttttgtcaga tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt
    13921 atttttttca taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt
    13981 atcagttttg ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac
    14041 agttttcgca agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct
    14101 ggcttaagta ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc
    14161 aggcctgtga cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc
    14221 cttccctgta tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg
    14281 tagggcgtgc tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa
    14341 ctttcatgaa agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt
    14401 tttgtaatct cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc
    14461 aaaaaaatcg tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt
    14521 gcaaagggct tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac
    14581 cgctggggac gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg
    14641 aggagtgcca ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt
    14701 tggcgctgcg ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc
    14761 atgttgagat taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata
    14821 aagccggtca gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg
    14881 cgatgtctcc gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac
    14941 tgaagtccct gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat
    15001 atactgcggg cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga
    15061 acaaaagcag caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct
    15121 gggccggtgt gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag
    15181 tcgtggtcta tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc
    15241 cgcagaataa actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga
    15301 ataaagttcc tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct
    15361 attcagaaac ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa
    15421 ccatgcgtgg tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat
    15481 gggtagactc cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg
    15541 ccggaacctg gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga
    15601 atgagtttga gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt
    15661 gcacagattg tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct
    15721 ttttttatta cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac
    15781 tcataaatcc tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag
    15841 gagcctttat gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg
    15901 agactcgctc tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt
    15961 cgcagttggc atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag
    16021 cctctttgaa aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc
    16081 tctccgcgca ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag
    16141 cgaatgcggt tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg
    16201 ggcaggatgg aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg
    16261 tggggctgtc ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg
    16321 tcccgggtct gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt
    16381 ctggaccgat ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg
    16441 ccgaaggtaa agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc
    16501 cgggaacatg tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg
    16561 ggatgcagtt gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga
    16621 ccgctgccgc tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga
    16681 tcggtaagat gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg
    16741 gtggttccac taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga
    16801 ttaactggga tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc
    16861 cgaacggtcc ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc
    16921 gtgaactgct caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc
    16981 tgtctcgtta tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg
    17041 cggaaaaatc actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg
    17101 gtgggacaaa agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc
    17161 cggttgagaa ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta
    17221 tgccggcctt tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg
    17281 ggccaaaagc gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat
    17341 tggaccggtg tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta
    17401 aagtgccgtc agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag
    17461 tgcgcgacgg ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag
    17521 acgaagcgga actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg
    17581 gaacaggacg tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg
    17641 caacctgtat cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga
    17701 aaaactggaa aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg
    17761 tggtaaaaaa actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc
    17821 gcgttctgga agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca
    17881 aagaagtgcc tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag
    17941 aagtcactga agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga
    18001 aagctgctac cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga
    18061 tgctgggtat ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg
    18121 gcgtgaaagc cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg
    18181 gtggtatttc tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg
    18241 gtggttcctg gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta
    18301 agctggcgcg tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg
    18361 atcgggcatt tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc
    18421 ggcgtgcttc gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc
    18481 cagatgacgg ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc
    18541 tacggcattc tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac
    18601 ggcagaagct gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg
    18661 tacatgcagg gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg
    18721 tggacgaggg gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc
    18781 aaacagacca taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc
    18841 aagggcaagc gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg
    18901 gcgatgacct actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt
    18961 aatttcgaag tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc
    19021 gccttgctga gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg
    19081 ccccttgccg gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac
    19141 cgcctcgcgg aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag
    19201 ctcttctgcc gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc
    19261 gcaggggaca acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc
    19321 ttcgatctcc ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc
    19381 accatcaagc atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc
    19441 atagcgatcg acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt
    19501 acccagttca ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc
    19561 taataacgtc ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt
    19621 tgcgtgtgtc tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa
    19681 tctcagttat tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga
    19741 gggcgaagag aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct
    19801 tgatacggcg gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga
    19861 gcaacgtgaa gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct
    19921 gaaaaccggc aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc
    19981 ctcacacgcg gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa
    20041 tcattgtgct atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt
    20101 ttctgcctgc gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc
    20161 aaagtgtgaa ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca
    20221 aaatcgcgtg gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc
    20281 ctgggtcagt ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt
    20341 gcgtggtaaa agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc
    20401 ggaatatcag cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc
    20461 gagtgccttg tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt
    20521 cgcatcgggc aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa
    20581 atacccttat cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac
    20641 ggcctaccgc cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc
    20701 atccgctatg ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta
    20761 aatcaactgc aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag
    20821 ggattgggca aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca
    20881 aataaatcag cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc
    20941 ggagcggtag cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat
    21001 caggacgaac cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg
    21061 atccttgcgc tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg
    21121 gcaatcgacc agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag
    21181 caggcaatcg aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa
    21241 gaggcgctgg aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa
    21301 cgcagttacc tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc
    21361 attgaacgcg atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat
    21421 agcctgaagt tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt
    21481 ttctctgtct gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac
    21541 gaccctgttt cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca
    21601 gaaagaacaa taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg
    21661 gcgatacgcg catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag
    21721 tcctggacta cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag
    21781 ccttgcagga tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg
    21841 cgccctacat aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg
    21901 ctatctacca gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg
    21961 atgccaacaa gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc
    22021 ccgataacac cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc
    22081 gttttcgtaa tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca
    22141 gctttgtctg ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc
    22201 tgctgcctta tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga
    22261 tctacgaaga aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact
    22321 atgtcgtcat tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa
    22381 tggccgatgc cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg
    22441 atcactacca gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat
    22501 accgtacccg tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca
    22561 tcatgctgga agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg
    22621 ggttaaccag tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg
    22681 atgatccggc ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc
    22741 gttatggtta ttcttccatg accacaccag acactttgtt tgaactggat atggataccg
    22801 gtgagcgtcg tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca
    22861 gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc
    22921 atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg
    22981 gcgcaagtat tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg
    23041 tctacgccat tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg
    23101 gtaaatttct gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt
    23161 taaaactggg ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca
    23221 tgttgatggg cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg
    23281 taccgtttgt tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg
    23341 agtttgaaga gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca
    23401 gcccgtatga caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac
    23461 acgattctca ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga
    23521 aaaccgatga ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat
    23581 ctggccgctt taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg
    23641 cgcagggaac attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg
    23701 ttaaacgcag ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg
    23761 gatagcgttc ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg
    23821 tcatcaacga cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt
    23881 tcataatatc gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat
    23941 gcaggcccag cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt
    24001 acttgagccc tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca
    24061 tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt
    24121 aatagtgtgg gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc
    24181 gatgaatcgc catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg
    24241 ggttgacgat ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca
    24301 aaccgcaggt ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta
    24361 gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa
    24421 tgaaccacga tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc
    24481 acgggcattt gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg
    24541 ggccgacgcc agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt
    24601 cgggtccata tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg
    24661 gcccagacat gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa
    24721 caccgcaatt ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt
    24781 cattcgctag gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc
    24841 aggaagggca cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag
    24901 ctgacattgt tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt
    24961 gagtgaggtt ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac
    25021 ttccttttca aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca
    25081 ggcgttatgt aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt
    25141 aaacgtgaca gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg
    25201 cgcaaatgtt ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt
    25261 tctttaaaag ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga
    25321 tccagtttag ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta
    25381 ctaagagtga gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa
    25441 ggttatgctc tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac
    25501 gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg
    25561 cacatgcgca tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc
    25621 aggcaatcac tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca
    25681 cggggccaaa aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa
    25741 agcaactgat tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc
    25801 atgttgtttc ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa
    25861 tcatgctggc gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg
    25921 tctttggctt tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga
    25981 cgaagcgttc tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg
    26041 caatgctcgc cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa
    26101 acatttggca ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc
    26161 tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt
    26221 ttatgctcac taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg
    26281 aaggggtaac agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg
    26341 cgtggtttgg gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc
    26401 gacactaggc tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg
    26461 gcgtactggc aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta
    26521 cgacttactg gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca
    26581 ttgcgctggc taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg
    26641 caccctggtt tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga
    26701 tcatcagcct gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa
    26761 gtattctggc gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga
    26821 taaccgtgag tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa
    26881 tgctgacttg ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt
    26941 atcctttaaa cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca
    27001 tttcggtgat tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt
    27061 ttatagaacg agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa
    27121 aaaaaccgcc tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca
    27181 ccgcgctttt tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc
    27241 ttaaagggca aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc
    27301 aagagccatt tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt
    27361 aaaatcaatt agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta
    27421 ttccggtctt ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa
    27481 atcagagcag taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg
    27541 atgtgtggcc aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc
    27601 cagccaggct tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt
    27661 aattttctgg ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg
    27721 ctgttttttc taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt
    27781 tcaactactg caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca
    27841 atcatcattc ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac
    27901 cgcatattcg ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat
    27961 gggaaccatg ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc
    28021 agccgttctc ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat
    28081 atacgtcctt caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct
    28141 gccatcgtgt ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg
    28201 gttatttctg tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc
    28261 cgagttttat aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca
    28321 atccagcctg ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga
    28381 gagtagtttt tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt
    28441 gaaattttac ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg
    28501 tctctccttc gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg
    28561 ttttaacttc tccattaacg gact
//
LOCUS       DAJTYL010000045        28328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYL010000045 DAJTYL010000000
VERSION     DAJTYL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.045"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..2109)
                     /gene="yccS"
                     /locus_tag="OO572_001902"
     CDS             complement(<1..2109)
                     /gene="yccS"
                     /locus_tag="OO572_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6660922.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLKRATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIG"
     gene            complement(2119..2565)
                     /locus_tag="OO572_001903"
     CDS             complement(2119..2565)
                     /locus_tag="OO572_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6660923.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2688..4742
                     /gene="helD"
                     /locus_tag="OO572_001904"
     CDS             2688..4742
                     /gene="helD"
                     /locus_tag="OO572_001904"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6660924.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4774..5232)
                     /gene="mgsA"
                     /locus_tag="OO572_001905"
     CDS             complement(4774..5232)
                     /gene="mgsA"
                     /locus_tag="OO572_001905"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6660925.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5328..5990)
                     /locus_tag="OO572_001906"
     CDS             complement(5328..5990)
                     /locus_tag="OO572_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6660926.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6163..6576
                     /locus_tag="OO572_001907"
     CDS             6163..6576
                     /locus_tag="OO572_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6660927.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6621..6938)
                     /gene="hspQ"
                     /locus_tag="OO572_001908"
     CDS             complement(6621..6938)
                     /gene="hspQ"
                     /locus_tag="OO572_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6660928.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6996..8186)
                     /gene="rlmI"
                     /locus_tag="OO572_001909"
     CDS             complement(6996..8186)
                     /gene="rlmI"
                     /locus_tag="OO572_001909"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6660929.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8281..8559
                     /gene="yccX"
                     /locus_tag="OO572_001910"
     CDS             8281..8559
                     /gene="yccX"
                     /locus_tag="OO572_001910"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6660930.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8556..8885)
                     /gene="tusE"
                     /locus_tag="OO572_001911"
     CDS             complement(8556..8885)
                     /gene="tusE"
                     /locus_tag="OO572_001911"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6660931.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8976..9635)
                     /gene="yccA"
                     /locus_tag="OO572_001912"
     CDS             complement(8976..9635)
                     /gene="yccA"
                     /locus_tag="OO572_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6660932.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9842..9929)
                     /locus_tag="OO572_001913"
     tRNA            complement(9842..9929)
                     /locus_tag="OO572_001913"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9893..9895),aa:Ser,seq:tga)
     gene            10355..11473
                     /gene="hyaA"
                     /locus_tag="OO572_001914"
     CDS             10355..11473
                     /gene="hyaA"
                     /locus_tag="OO572_001914"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6660933.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11470..13263
                     /gene="hyaB"
                     /locus_tag="OO572_001915"
     CDS             11470..13263
                     /gene="hyaB"
                     /locus_tag="OO572_001915"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6660934.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13282..13989
                     /gene="hyaC"
                     /locus_tag="OO572_001916"
     CDS             13282..13989
                     /gene="hyaC"
                     /locus_tag="OO572_001916"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6660935.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13986..14573
                     /locus_tag="OO572_001917"
     CDS             13986..14573
                     /locus_tag="OO572_001917"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6660936.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14570..14968
                     /gene="hyaE"
                     /locus_tag="OO572_001918"
     CDS             14570..14968
                     /gene="hyaE"
                     /locus_tag="OO572_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6660937.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14965..15822
                     /locus_tag="OO572_001919"
     CDS             14965..15822
                     /locus_tag="OO572_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6660938.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15956..17500
                     /gene="appC"
                     /locus_tag="OO572_001920"
     CDS             15956..17500
                     /gene="appC"
                     /locus_tag="OO572_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6660939.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17512..18648
                     /gene="appB"
                     /locus_tag="OO572_001921"
     CDS             17512..18648
                     /gene="appB"
                     /locus_tag="OO572_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6660940.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18660..18752
                     /gene="cbdX"
                     /locus_tag="OO572_001922"
     CDS             18660..18752
                     /gene="cbdX"
                     /locus_tag="OO572_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6660941.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18832..20130
                     /gene="appA"
                     /locus_tag="OO572_001923"
     CDS             18832..20130
                     /gene="appA"
                     /locus_tag="OO572_001923"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6660942.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20245..22411)
                     /gene="etk"
                     /locus_tag="OO572_001924"
                     /pseudo
     CDS             complement(20245..22411)
                     /gene="etk"
                     /locus_tag="OO572_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22431..22877)
                     /gene="etp"
                     /locus_tag="OO572_001925"
                     /gene_synonym="yccY"
     CDS             complement(22431..22877)
                     /gene="etp"
                     /locus_tag="OO572_001925"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6660943.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22865..24004)
                     /locus_tag="OO572_001926"
     CDS             complement(22865..24004)
                     /locus_tag="OO572_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6660944.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24050..26146)
                     /locus_tag="OO572_001927"
     CDS             complement(24050..26146)
                     /locus_tag="OO572_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6660945.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26146..26892)
                     /locus_tag="OO572_001928"
     CDS             complement(26146..26892)
                     /locus_tag="OO572_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6660946.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26889..27533)
                     /locus_tag="OO572_001929"
     CDS             complement(26889..27533)
                     /locus_tag="OO572_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6660947.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27640..27945)
                     /locus_tag="OO572_001930"
     CDS             complement(27640..27945)
                     /locus_tag="OO572_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6660948.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         6827 a         7196 c         7478 g         6827 t
ORIGIN      
        1 accaatctct ggcagtaatg caatcaataa cccaacttgt tgtacgacca gaggttcttt
       61 gctatctgcc cgtggttcaa gttgctgcat ccgctgtttc aggccagcta atgcctgatt
      121 gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc
      181 atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga
      241 gatatagctg gtaaacgtat ggttaaggca cagcaagcga aacgcggctt cgcgaatttg
      301 cggggtaacg ttcggttcgc tggacatatt tgataccacc gacgccagtt cagcatcacg
      361 gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta
      421 ttgctccagt atggcatcga gatagcgaca gttggcctct gtggcgcgtt tgagcattcg
      481 cggcagattg cgaaacttcc agtcaggcca gatgtagctc actgccgccc acgcaatggc
      541 acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa
      601 gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt
      661 acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga
      721 tggcacaaac cacagcacag gaatgccaat cgcgatacct accagcgtac caataatcct
      781 taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag
      841 caagatccaa tacccgtgat gcattccggt tatttgaatg atggcgtagc cgaagcacaa
      901 caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct
      961 aagacgcagc cagatatcac tcaatccgtg cgggctgtca tcagcgagct cattttcgtc
     1021 attattacgg ggtagtgtct gggcctgttc tgattcaatt gttgccagtt gggcatcaat
     1081 ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt
     1141 atcgcgtatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc
     1201 atgttgataa gactgacgca acaaaataca gcgtgacagt tgctggcacg cctggccctg
     1261 catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa
     1321 tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg cgacaaagta
     1381 ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag
     1441 cgagagtttc gtctgattca atgtcgccat cagctgaccg ttggcgagag ccaaatcgta
     1501 cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag
     1561 ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa
     1621 cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat
     1681 cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag
     1741 caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc
     1801 gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac
     1861 tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc
     1921 cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt
     1981 cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat
     2041 acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat
     2101 aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg
     2161 cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt
     2221 aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc
     2281 aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt
     2341 accgccagta ttcagcaaca catttttgcc agccgggttc agttcatcga catgaatagc
     2401 ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg
     2461 taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc
     2521 aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac
     2581 tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc
     2641 tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag
     2701 cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg
     2761 ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg
     2821 cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa
     2881 aagtggtgcg tctgcacggc acagaatggg gcgagacgca gcgtttttac catcatcttg
     2941 atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc
     3001 agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa
     3061 cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc
     3121 gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggct tggttgaaag
     3181 atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg cttaccgagt
     3241 atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag
     3301 tcgttaatgg cgagcattct ctgttagtgc tggctggtgc aggaagcgga aaaacgtcgg
     3361 tgctggtggc ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct gagcaaattt
     3421 tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt cgcgaacggc
     3481 tacataccga agacattacc gcacgcacgt ttcatgcgtt ggcgctgcat attattcagc
     3541 agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg
     3601 aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct
     3661 ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg
     3721 atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt ctgatgcgga
     3781 tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt
     3841 tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg
     3901 cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt ctggagaaag
     3961 gccgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc
     4021 agcgcgcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg ttgttcgccg
     4081 ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg
     4141 ctttccatga aaactttggt gaaggcgatc gctgtgattt agacacgact taccgtttta
     4201 acagtcgtat cggtgaggtg gcaaaccggt ttattcagca gaacccaggc cagctgaaaa
     4261 agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg gatgagagtc
     4321 aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga
     4381 tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc
     4441 cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg
     4501 tcatcatcgt tggcttgcag gaggggagtg gtggttttcc ggctgcggcg cgggagtcga
     4561 ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa gaacggcgtt
     4621 taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt aacaaagaga
     4681 atccctctcc ctttgtggaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt
     4741 aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtcag cgagataacg
     4801 ctgataatcg gggataagaa tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa
     4861 gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa
     4921 ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga atatcaatac
     4981 atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact
     5041 caacatcgcg ttgacgttca tgccggtcgc gcgggaaatc aagttaccgg tagtgcctgt
     5101 tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc tcatcagcat
     5161 ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt
     5221 cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt aagcacttat
     5281 cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt
     5341 tgtttttccg cccattgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa
     5401 tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa
     5461 acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg
     5521 cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt
     5581 gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat
     5641 tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag gcgaggcagg
     5701 cgaaaattga cctggttgat gagctgggta ttaaaactga ccaccagcgg tggggagata
     5761 tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga
     5821 tgcggtccat tgtccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc
     5881 ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg
     5941 gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc
     6001 tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac
     6061 atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat ggttggcatg
     6121 tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga aaccgatatt
     6181 gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat
     6241 cgccccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt cattccggtt
     6301 tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac gctggcagac
     6361 gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg
     6421 gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat
     6481 gagcaagcgg cagtactggc acgggatgcc gggttaaatg tggtgatgga tcgctgcccg
     6541 gctattgaga tccctcgcct gggtctggcc aaataaaaaa tccccggaag gcaaaaacct
     6601 tccggggatt tgttcaggga ttagttacgc agacgcgggg cctggagttg tttgcggatg
     6661 gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt
     6721 agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg
     6781 tgataccacg gagcggcgcg aagctcgtca ttcaccgcca gctcatcggg cgacggttca
     6841 gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg
     6901 cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt
     6961 acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct
     7021 ttcagatata gcccttccgg gtaggtagcg atcaccggat gatcggctgc ctgacggaac
     7081 tgctctataa attgtacatc acgaccggca tcaattgcgg catccgcgat gattttctga
     7141 aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc gccttcattc
     7201 agcaactgaa tcgccagcat gttgatgtct ttataaccac gacacgcgcc catcaactgg
     7261 cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga
     7321 tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga
     7381 tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg ggaggtatca
     7441 acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag
     7501 gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag gcggctgtct
     7561 cgctggtcca gatagtagcc cgttttgtgt ccgtgctgaa tatccaccag cagcttcatg
     7621 ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag gccctgggtc
     7681 agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc
     7741 gggtacagcg tttgcagggc actaattaat gccgcgcgct gatattctgc gccagcactc
     7801 agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca
     7861 gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc
     7921 catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg
     7981 tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac
     8041 cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt accttccatg
     8101 cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt ttcgcgccct
     8161 ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg
     8221 tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca
     8281 atgtcgaaaa tctgcataat tgcctggatt tacgggcgag ttcagggcgt aggatttcgc
     8341 tacaccacac agtacgaagc gaaaagactg ggcttaaccg ggtacgccaa aaatcttgac
     8401 gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtagaaaa attaatgcag
     8461 tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat
     8521 catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac cggttttggc
     8581 aggccagcaa ttttggtggc ttgcttagcc gggcctttcg ggaacagtcg gtataaatag
     8641 cggctgttac ctttttcttc accaaattta ttcgccatcg cttttaccag catacgaatc
     8701 gccggagacg tattaaattc caggtagaag tcacgtacaa aacgcaccac ttcccagtgt
     8761 tctggcgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg ttcactccac
     8821 tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc ttcgaagatc
     8881 agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc
     8941 ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc gaagcccaga
     9001 atgctcagta ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga
     9061 atatagttcg tttcaccgcc atgaatgatg ttgctggttt caaacaagat agcgccagag
     9121 gagatcagaa tgaagaccgc gctgatcgcc agatgcagag caggcagttg caggaagata
     9181 ttagcaatca taccaatcag taccaccaca atacccgcca tcagcatacc gccgaggaac
     9241 gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac taacgccgtt
     9301 ccgcccagtg ccatagcgat tacgtcaccc attccggcag acagataggt gttcagaatt
     9361 ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc
     9421 ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag agtcagaatc
     9481 agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc
     9541 agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagtgat
     9601 gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta
     9661 attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac
     9721 gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc
     9781 ctgagaattc cgacaggcaa aagaaaaagg gggtagcgtt tagctaaccc cttatcttat
     9841 ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc
     9901 ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc
     9961 ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc
    10021 gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta
    10081 gcaaccggct aaacatcccc atcgcccggc taaagaaaaa taggtccatt tttatcgcta
    10141 aaagataaat ccacacagtt tgtattgttt tgtgcaaaag tttcactacg ctttattaac
    10201 aatactttct ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc agcatctcac
    10261 gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc cgaccataag cgcccggtgt
    10321 cctgccggtg ttgctaggag aagagacgtg cgatatgaat aacgaggaaa cattttacca
    10381 ggccatgcgg cgtcagggcg ttacccggcg cagctttctc aaatattgta gtctggctgc
    10441 cacgtcgctg ggattaggcg cgggaatggc accaaagatt gcctgggcgc tggagaacaa
    10501 accgcgcatt ccggtggtat ggatccacgg tctggaatgt acctgctgta ccgaatcttt
    10561 tatccgctcc gctcacccac tggcgaagga cgtcatcctt tccctgattt ccctcgatta
    10621 cgacgatacc ctgatggctg ccgccggaac ccaggcggaa gaagtctttg aagacatcat
    10681 cacgcaatat aatggcaaat atatcctcgc agtagaaggt aatccgccgc tgggcgagca
    10741 ggggatgttc tgtatcagca gcggtcgacc gtttattgag aaactcaaac gtgccgctgc
    10801 cggagccagc gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc
    10861 gcgacctaat ccgacacagg caacgcctat cgacaaagtc atcaccgaca aacccattat
    10921 caaagtacct ggctgcccgc cgatcccgga tgtgatgagc gccatcatta cttacatggt
    10981 gacctttgat cgcttgccag atgtcgacag aatgggtcgt ccactgatgt tttatggtca
    11041 gcgtatccac gataaatgct atcgccgcgc tcacttcgac gccggagagt tcgtccagag
    11101 ttgggatgat gacgctgccc gcaaaggtta ttgcctgtac aaaatgggct gcaaagggcc
    11161 taccacctat aacgcctgtt cctccacacg ctggaatgat ggcgtttctt tcccaatcca
    11221 gtctggtcac ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta
    11281 cagccgcgtg gtcgatattc cgcaaatggg tactcattcc accgccgata ccgtcggttt
    11341 aaccgcgctt ggcgtggtgg cagcagctgt tggtgtgcac gcagttgcca gcgccgttga
    11401 ccaacgcaga cgtcataacc agcaacctac agaaaccgaa catcagccag gcaatgagga
    11461 taaacaggca tgagcactca gtacgaaact cagggataca ccatcaataa tgccggacgc
    11521 cgcctggtgg tcgacccgat tacgcgcatc gaaggccaca tgcgctgtga agtgaatatt
    11581 aacgatcaga atgtgatcac caatgccgtc tcctgcggca ccatgtttcg cgggctggag
    11641 atcatcctgc aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc
    11701 gtctgtactg gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc catcggtatt
    11761 aaagtgccgg acaacgccaa tatcatccgc aacattatgc tggcaacgct ctggtgccac
    11821 gatcatctgg tgcacttcta tcagcttgcc gggatggact ggatcgacgt gttagatgcg
    11881 ctgaaagccg acccgcgaaa aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa
    11941 tcatcccctg gctatttctt cgacgtacaa aatcgcctga agaaattcgt tgaaggcggg
    12001 cagttgggga tcttccgcaa tggctactgg gggcacccgc agtacaaact gccgccagaa
    12061 gccaacctga tgggctttgc ccactatctc gaagctctcg atttccaacg tgaaattgtc
    12121 aaaattcacg cggtctttgg cggtaaaaac ccgcatccaa actggattgt cggtgggatg
    12181 ccttgcgcca ttaacattga cgaaagcggc gcggtcgggg cggtcaatat ggaacgcctg
    12241 aacctggtgc agtcgattat cacccgcacg gcagacttca ttaacaacgt gatgatcccc
    12301 gacgccttag ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac gggtctttct
    12361 gataaatgtg tcctcagcta cggcgcattc ccggatattg ccaacgactt tggtgagaaa
    12421 agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt gctgccagtg
    12481 gatttggttg atccgcagca ggtgcaggag tttgtcgatc acgcctggta tcgttatccc
    12541 aacgatcagg tcgggcgtca tccattcgat ggcattaccg acccgtggta caaccccggc
    12601 gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg ctactcgtgg
    12661 atcaaagcgc cacgctggcg cggtaacgcg atggaagtgg ggccgctggc acgcacgtta
    12721 atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg
    12781 ttgaacctgc cgctttccgg tatccagtca acgttgggcc gcattttgtg ccgcgcgcac
    12841 gaagcgcagt gggccgcagg taagttgcag tatttcttcg acaagctgat gaccaacctg
    12901 aaaaacggca atctcgccac tgcttccact gaaaaatggg aacctgcaac ctggccgaca
    12961 gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca ctgggccgcc
    13021 attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg gaacgccagt
    13081 ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa caccaaaatg
    13141 gcgatccccg agcaaccgct ggagatactg cgtactctgc acagctttga tccgtgcctc
    13201 gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt gcaggtgcgt
    13261 taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag ccactatgtc
    13321 tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc ggtgttgatg
    13381 gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc gacgtatctg
    13441 ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt taccgtggtc
    13501 ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga gctgtttatc
    13561 gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg
    13621 tttctggcaa aacgtccgag tgccgatata ggccataacc ccatcgccca ggcggcgatg
    13681 ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct gtacagcgaa
    13741 cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt ctactggacg
    13801 ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct gattggggcg
    13861 tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga cgacacggtg
    13921 atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag taacaaggag
    13981 cgttcatgag cgagcaacac gtggtggtca tggggctggg caacctgctg tgggccgatg
    14041 aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg cccgagtatg
    14101 tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc gaaagcgcca
    14161 gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg ctgcgaacct
    14221 atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg catcagaaca
    14281 gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca catattgccc
    14341 tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc gaactggcac
    14401 gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg tggggaagtg
    14461 tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg atggaaaatt
    14521 acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga tgagcaacga
    14581 cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc cggtcagtga
    14641 atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat taagcagtga
    14701 cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat tactgcacga
    14761 gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg aagccatcgg
    14821 cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg gaaactatcg
    14881 cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc gcgggcttgt
    14941 cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct ggggccagga
    15001 acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg tcaggtgaat
    15061 gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt gattgcgctg
    15121 ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga ggtgctggca
    15181 gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct tctcggggaa
    15241 ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat acaggaggcg
    15301 atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa
    15361 ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg
    15421 acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc gttggcgcat
    15481 gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa tctgacgcaa
    15541 ttacccatca gcgaggctga tcggcttttt ctctcgcgtc tctgtgggcc gggaaatatt
    15601 cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt acgccatgtc
    15661 tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta tgaaatctgc
    15721 ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc gcagcggctt
    15781 agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc
    15841 tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt tcaggataaa
    15901 gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag tgcgcatgtg
    15961 ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc actttttatt
    16021 tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca tttacgtggt
    16081 taccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct tcggtatcaa
    16141 ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta ctaactggtc
    16201 attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg aagcattaat
    16261 ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc aacggctgaa
    16321 taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc tctctgcatt
    16381 gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt ttgatatcga
    16441 caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg tcagccaggt
    16501 gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta ttatggcgat
    16561 cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct cgtttgccat
    16621 cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag acagttctgc
    16681 gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg agtggcaaac
    16741 ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc aagagcgtaa
    16801 cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact cattagataa
    16861 acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc aacgcggacg
    16921 tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc atgtgttgca
    16981 ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc gctatgcgcc
    17041 ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg cgatacctca
    17101 ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc tgctgctact
    17161 ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc atcgctgggt
    17221 gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag ccgggtggtt
    17281 tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga catactccgc
    17341 gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg tagggcttta
    17401 caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc tggggccgag
    17461 cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat catgtttgat
    17521 tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt ggtctttatg
    17581 atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc gcgtaatgat
    17641 gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa tcaggtctgg
    17701 ttgatcctcg caggtggggc attatttgca gcctggccca gagtgtatgc agcggcgttt
    17761 tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg tccgctggcc
    17821 tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga tgccggtctg
    17881 gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa cttgttgctc
    17941 ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag cttctggcaa
    18001 ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt gattttgcaa
    18061 ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc acagctggcg
    18121 actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta ctggctgtgg
    18181 gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc ccatccgtta
    18241 atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga atcgcccgtt
    18301 ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat ggcgatttat
    18361 cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg cgtgattttc
    18421 acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc aatctccagc
    18481 ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt ggtaatcgtg
    18541 ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa aatgtgggga
    18601 cgcatgacaa cagaaactct ccgccgtaac aaaaacgagt tgtactaagg agcaggaaca
    18661 tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt
    18721 tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta ggcctgatgc ggcgcattag
    18781 catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc gatgaaagcg
    18841 atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc attcgctcag
    18901 agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg tgtgcgtgct
    18961 ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc aacctggccg
    19021 gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct cggacattac
    19081 caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc gcagtctgct
    19141 caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga agccttcgcc
    19201 gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac gtccagtccc
    19261 gatccgttat ttaatcctct aaaaactggt gtttgccaac tggataacgc gaacgtgact
    19321 gacgcgatcc tctgcagggc aggagggtca attgctgact ttaccgggca tcggcaaacg
    19381 gtgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg ccttaaccgt
    19441 gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact caaggtgagc
    19501 gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac ggagatattt
    19561 ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac cgattcacac
    19621 cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca acgcacgccg
    19681 gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc gttgacgccc
    19741 catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct gtttattgcc
    19801 ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg gacgcttcca
    19861 ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg gcgtcggcta
    19921 agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca gcagatgcgt
    19981 gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac cctggcagga
    20041 tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca aatcgtgaat
    20101 gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca gttacctgaa
    20161 tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa aaggcgttca
    20221 cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac cgtaatagtt
    20281 atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga ttgcgccttt
    20341 aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt tggcggtgtt
    20401 caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga ccgcagcatt
    20461 gctcaccgcc agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt cgttcgccca
    20521 ttccagtaat tgacgcatcc gatcgcgcat cagcagttca gacgggttag gtggcacctg
    20581 accgcgagta atcacatcaa agtctccttt gccaaaatgc tggatcactt tgttgagctc
    20641 atctttacct gccagatatt ccgacaagcc atgttcatta ctcacggtaa acaggttatg
    20701 cgaataacca cggcgtaagt cggcatcaat aaataacact ttttgatcgg actgggcgat
    20761 caccgctgcc agagttgaac tgacaaacgt tttaccactg tctggcgtcg caccggtgat
    20821 catcagaatg ttattctcag tctccatcat agcgaaatgc agactggttc gtagcgcacg
    20881 tacggcttcc acagcagaat ccgccgggtt atccaccgcc aggaagggga tatttttagt
    20941 acgatggcgc tgctgattag aaaataaatt tttcttacgc agacgggtgc gtttatccag
    21001 ccactcggac attgggatag tggaataaac gctgatgccg tgctcttcca gttgttccgg
    21061 ggcttctaca ccacgacgca acatcgcacg cgccagcacg gcactcaccg aaataaacag
    21121 gccaagaata aaaccaagca ccacattcaa cgcttttttc ggtttcactg gctgcggctg
    21181 agtgactgcc gggtcgataa tccgcacgtt accaatggca ctggatttcg aaatactcaa
    21241 ctcctgctgg cggttaagta attgcagata taccgcacgg cccgcttcta cgtcacgact
    21301 taaacgcaac acttcctgtt gggtggaagg cattgccgat acccgcttat tcaggcgttt
    21361 gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg ggtgatcttt
    21421 cttatacagc tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt gattatcaac
    21481 gttcacaatc tgctcaagaa cggctttggc ttccaggtta aggtcaaccg aatcgcgctg
    21541 ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca cttcaggtaa
    21601 ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag cgatattctg
    21661 ttgcaaatag ttgttagcga tgctgttcag aatacgagta atcaactggg gatcatcacc
    21721 agtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg taaaggtttc
    21781 ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc aggacaaact gtgttcctgg
    21841 tttggcctta atgtccgcga tagtcagcgc aacgccatct ttttccagac gctggccgac
    21901 cataccattg acggtgaact cttcaccttc cagtgtatag tggccgtttt ccccaaccgt
    21961 gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc tgatcgccag
    22021 ctcacctagt ttttctttgg ttaatctcgc ccagccgcga cccacaatcg gaaaatactt
    22081 ctgctcaact atgtcgcgca gattcagttc agcaatggtt ttaccgagaa tcatgcgcga
    22141 ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca tatcgctcag
    22201 gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat ctgcctgata
    22261 aattggtgtg cttaacagcg atcagcgtga ataacgcggt cacgctgata ataaacttac
    22321 ggtgatccca taactcgccg accagacgaa gcagatcgat ctcattttcc tgagtgctgc
    22381 ctggtggcgt attcatattt ttagttgtca tcgtatttta taaactcaaa ttaccggctg
    22441 aggcgcttcg cccattcctg actggcgcgc tccaacatac tgtagacatg ttcaaatgcg
    22501 tcctgacttt tacgataggg atccgggatc tctttctgtt ccagccattg cccaaacagc
    22561 attgtcttcc cgcggacctc gggagcaatt gcggtaacct gagcaatatg ttccgactcc
    22621 attgccagaa tcagatcgta atttcgtgcc atctcagcag tgagcttacg tccggcatgt
    22681 ccttcaagag atacgccatg attagcggca acatctgccg ctgtcgcatc tgcagggtgt
    22741 tttaccagac catgaacacc tgccgatttg acttttacgc ccggcagacg tttacgcagc
    22801 aagcgctcgc caatgggcga acggcaaatg ttgccggtac aaaccactag gattgagtta
    22861 aattttagtt gggccatgtt tgaatccacc gtgaagtctc tgtcaggtca tgcaccccgg
    22921 aaatcgtcgg taccagcaga gaaatcacac ggttccaacg agccagcggc gcggtagtga
    22981 cataaacaat gtcgtaaggt tcaagctgga attccgttcc gagtatcatt gccgatgcgt
    23041 ccttcgcatt cagctgataa atattggcga ttttgccatt ctgcttgttt tgcgttgcac
    23101 gaataacaaa aatccccgtc gcatcagcga catcctgatt cataccctca gcgttaccca
    23161 gcgcttctgc cagggtcata ccgctgcggt ccattttcag cgtgctctgt ttacccactt
    23221 cacccatcac gaagactttc agatcatcgt tgcgcggaat aaataaaata tcgcccgggt
    23281 gcagcagttt gttctggcgc aagtcgccac gctgcatcag cgcatagagg ttcactttgg
    23341 tctttacgcc attctgggtg agcaccacgt tgcgccagtc tgcatctgcg gtcagaccgc
    23401 ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt ggtaatcggc tgctgacccg
    23461 atttgctgac ttcgccggtg acgtacgctt tttgcgatcg gaacgccgcg acgctgacat
    23521 cgacttgcgg gctttcaatg actgaatcga gacgcgctgt aatctcgttg cgtacctggg
    23581 taagtgtttt cccggccact ttcaggcgac caatataggg gtagaaaatc gcaccatcgg
    23641 cgttaaccca gttgccggta tcactggcgc tacggtattg ccctgcgggg gtggttagtt
    23701 ccgggtgatc ccagaccgta accatcaaca catcaccaat cccgatacga tattcatagt
    23761 ttgcgatttg ctgctccagc tccgggttag cctgcgacat aatcggtttg gcgcgcagct
    23821 gatcaatcaa tcctggcgtg acgggataga tattgaccat tttgtccagg tcatactgat
    23881 tgtcaggtag ttcaatgaca tccttgttac tggtcgataa attctgacca ggaacaaggg
    23941 tacatgctgt caaagacaga acagccaatg tcagtaccga aaacttaaaa atgttctttt
    24001 tcattggttt atatttatca gtcaatgtaa tgttatgaat tacgtcaggt tattgtccaa
    24061 ccggtacttc tcgctctgcc gtcattggat cgagctggta tttgcgtgac agcatttgtc
    24121 caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg gttgcggtta ggtccaatgg
    24181 tcatcaagtc gaacggaatc gagatataaa aacctttact aaagccgcct tcgccgtagt
    24241 catctttaga tacgttactg attgccgccc atacccctac cgccacgccg ctgtcaaaga
    24301 gtttggcgac gtcgatcgtt gcgcctttat cttttgccag atattgccca acgctaagtt
    24361 tcatcagtac gccattgagc gtcggcgggt tccagtaagc cgtcacgaaa cctgtgggcg
    24421 tggaataatc ggtgaagcgc atcatgttgt cccagtcacg ctgcttcacg tagttaacgt
    24481 ccacacccag cgcccagctg gcatctagcg ggcgataaag aagctcggaa ccgacaccgg
    24541 catacatcgt ttccagataa ccgccataca cctggccata gaaaccattg cctaagtcgg
    24601 caaagtaatt cgcttgcaag ttgttgagat aaacgtcatt gcgaacgtaa tcacggatat
    24661 gcgtgcgcac gcggggcagg gtagagtccg cgggtaacag tgaagactta aacttgtcgt
    24721 agttgttgta aatattggtg aaaataccgt catcaagcag caggtggtcg gtaaaccagt
    24781 agcgggcgct ggacatcagc cccagctgga acatatagaa atcttccggg ccgcccagcg
    24841 actgtgaaag tgttgggttg aaagagtagc taaagcgatc ttcacgaata cggtagcacc
    24901 gaccaaaggc agaaagatct tctgcttcaa cacgttgttg ttgcagttgc tctgatttac
    24961 caggcgctgt gccggccagc tgtttgcgca ggctggctac gtcggtttcg gtagtcacca
    25021 tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc aacgccttgc ggcaggttat
    25081 tcaccagaat ccggttggca cgatcgaccg cttcgcgaga gtcacggtat ttgtattgct
    25141 gaccagacat atacagcgtc ttatcgcgta gctgaatttc tggcgcgtca aagcccgcgt
    25201 tatacttcag ggcggtaagt tggtttgcta ccgtggtgta ctgcaaacct tcagactctg
    25261 gcgcaggctg ccaggccggt tttggcgtat cgcgtagggc agggcgcaga tcgttgaaat
    25321 tggtccgtaa agtgaaacca aacatcaacg tgtttccgcg ttcataactg aggttgaggt
    25381 ctgcccagct ggcagcgcga taaactgcac cgacgttgaa atggcttgcc tgaggcagtt
    25441 tgccagcgaa atcattctgg taattgttgc cgtcgtattc gagtttcaga cgcaggggat
    25501 tccacggcgt ttgatactca atgccgccaa agatggaagc cgggccacga aagatatcgc
    25561 taaagctgat atcgcccgca tcgtgagact ctgcgcgatg acaatattta tcgctcaccc
    25621 ggcaaaacgg gttggtaata ttgcccgcat taccggcgta gccccatgcc atcccgaggg
    25681 tgaaatcaaa tggccccgct tgcttgctgg ccaccagata ttcaccgtca aacaaacccg
    25741 taccagcaat atcacgttta ccaaacgcca cttgcggtag ccagtaacct tcttcccaca
    25801 ggcgaagttt aaaatcgaat gatttgtctt tgtatgactg atcgccgctg aaatcttccc
    25861 actggctata tttgcgtgtg cgcacatccg tataacgaat ggtgccttcc agccatggga
    25921 acagcgccac ggaggtggag tagaaccggt attgatcgtt atcccggtag ttgacgctga
    25981 attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa caatcctgtg ccgccaaagt
    26041 ctgattgcga cgaacccagc ggatccgggt aggttaatac ttcagcatga caggcactgg
    26101 agacggcaat ggccaggcag cttaaaagat aagaattctt cttcatcagt caggaacacg
    26161 ttgcgttaaa acagacacaa tctggtcatt taaatcagca tatttttcgg gcagtacatg
    26221 cgcagaaaag cccaaccaaa gctggctgcc aggcggtggt tccacatgtc ttttattcca
    26281 cagggcaaca ggggcgacga cagtctcacc ctcaggcgta atcaccataa cgttattctt
    26341 gtctgcgcct gcaaggcggg ggtgatcttg cagataatcg gtaacgctac ggcctgcctg
    26401 ccagggcaat tgcccagccc cagaaaccgc acccagcaga gtaatagtca ccggacgctg
    26461 tacggtatag agcgtatagt ccccgaccaa cgggggattg ctgttctcat ccactcgtac
    26521 aaaatcaggg tccagtttga ccggtaagcg accagtgata ttgagattga gtaactgctg
    26581 gcgcacggat ttaatcgttg ccgccacatc gtcgtcagct tctgcttccc aggaagcaag
    26641 ctgtgccatg acatgttggt agtctttaag cgctttagct ttcgccgcag agtcggtcag
    26701 caaggcacca ggccaccaga ggcgatcgcg cagttgcggt tgtgtcacca actggacaac
    26761 attttccacg gggccaacgg aaagtgtctg ttgttcgcta ggcagataaa tagtcaccgt
    26821 tccttgcgca aaggcatggg gtgtcattac gtaaagtacg ctggcaatga aatacgactg
    26881 taatttattc atggtgcggg tttaagaaac gtcatttcta cgggaataac ccctgcgcct
    26941 aacatttgcc gactttgacg aacctggcca gtagccgaat cgacccagaa tgtgttttgc
    27001 cactgttttt ctggcagcgt ggaagtcacc tcttcctgcc agacattaca cagcacattg
    27061 tgacccgcaa tattcagcgt ctctaatcca gcaaaggaaa aagtagaact tagggtcgca
    27121 gagcgaaaat ggttgtcttc actccagaga atatcgcgcg tccagcggct gccatctttt
    27181 attgccagcg cgttacgcag aggggcccgc ccgggatttg tcacttccag cagattatta
    27241 ttaagtttga cggtctttaa aagacgtcca ttgtgggtaa ccagcatggc gttatcctgg
    27301 gataaccatt tgctttgttc ttgttctata tatcccagta ccacaaagat tcgttgccct
    27361 tcattaaggc gtaaatacat cgtggaataa ggcaacgcct ggatctgctg agccgctaca
    27421 gtgatatcct gattatcgaa aaggctggcg cgaaatgtat cgaccatact ttgctggctg
    27481 tgcgtacatc ccgcaagaaa tagtgcgaaa atcgataaaa taagagggcg cacctttaat
    27541 ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat atcgcaaccg
    27601 ggtattattt aacttgaatt tcgacgcgtt taagcagaat tagcgcgtag tggacgtggt
    27661 ggttgaggtg ttatgagaac cgccatcgcc actgtcattt gctgcaacta ctgccaacgt
    27721 cgccacacca acagccgttg ccacaccgac aactgtcgcc gtgccgacag cgccagcagc
    27781 cacgccacta gtggtagccg cagtcgtggt tgcacctgta gtggtcgttg tggtgccagt
    27841 agtacccgtc gttgcctcgg tcccgtttgc tgcttcaggg gcagcaaacg cagctggcgt
    27901 cgtcaacatg aaggccataa ggattgcaga aagcttgtgt ttcataactt ttcctttatt
    27961 catcgcatgg acaatacgta cgcttgagat caggattgtc tactttgagt ataacatcct
    28021 tgtgaaatct ccctttcctc ataccgctag cactatatac tgcgggtggg aaaggcgcgc
    28081 agagggaaat ataagattgt taacgatttc cctctggaaa tacgacttgt gagcataaga
    28141 taaatcttag aaaaatacga attaaagata agaaaattgt tacgaaagct attaattatt
    28201 gttggtaata tagtttcaag tggtactata actggctgtt aaatggcatt tataactatt
    28261 aggtgcagag atattcgctt aaatggatta gtttcttagt ggaaaagtaa ctgatgttat
    28321 aatgtatg
//
LOCUS       DAJTYL010000046        27893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYL010000046 DAJTYL010000000
VERSION     DAJTYL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.046"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(101..1117)
                     /gene="phnD"
                     /locus_tag="OO572_001931"
     CDS             complement(101..1117)
                     /gene="phnD"
                     /locus_tag="OO572_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6660949.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1142..1930)
                     /gene="phnC"
                     /locus_tag="OO572_001932"
     CDS             complement(1142..1930)
                     /gene="phnC"
                     /locus_tag="OO572_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6660950.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2063..2506)
                     /gene="yjdN"
                     /locus_tag="OO572_001933"
     CDS             complement(2063..2506)
                     /gene="yjdN"
                     /locus_tag="OO572_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6660951.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2664..2999)
                     /locus_tag="OO572_001934"
     CDS             complement(2664..2999)
                     /locus_tag="OO572_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6660952.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3401..5629
                     /gene="crfC"
                     /locus_tag="OO572_001935"
     CDS             3401..5629
                     /gene="crfC"
                     /locus_tag="OO572_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6660953.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5626..6504
                     /locus_tag="OO572_001936"
     CDS             5626..6504
                     /locus_tag="OO572_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6660954.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6768..8270
                     /gene="proP"
                     /locus_tag="OO572_001937"
     CDS             6768..8270
                     /gene="proP"
                     /locus_tag="OO572_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6660955.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            8382..8471
                     /gene="pmrR"
                     /locus_tag="OO572_001938"
     CDS             8382..8471
                     /gene="pmrR"
                     /locus_tag="OO572_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6660956.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8447..9547)
                     /gene="pmrB"
                     /locus_tag="OO572_001939"
     CDS             complement(8447..9547)
                     /gene="pmrB"
                     /locus_tag="OO572_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6660957.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9548..10216)
                     /gene="basR"
                     /locus_tag="OO572_001940"
     CDS             complement(9548..10216)
                     /gene="basR"
                     /locus_tag="OO572_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6660958.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(10213..11856)
                     /gene="eptA"
                     /locus_tag="OO572_001941"
     CDS             complement(10213..11856)
                     /gene="eptA"
                     /locus_tag="OO572_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6660959.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(11960..13297)
                     /gene="adiC"
                     /locus_tag="OO572_001942"
     CDS             complement(11960..13297)
                     /gene="adiC"
                     /locus_tag="OO572_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6660960.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(13434..14195)
                     /gene="adiY"
                     /locus_tag="OO572_001943"
     CDS             complement(13434..14195)
                     /gene="adiY"
                     /locus_tag="OO572_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6660961.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(14520..16787)
                     /gene="adiA"
                     /locus_tag="OO572_001944"
     CDS             complement(14520..16787)
                     /gene="adiA"
                     /locus_tag="OO572_001944"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6660962.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(16986..17894)
                     /gene="melR"
                     /locus_tag="OO572_001945"
     CDS             complement(16986..17894)
                     /gene="melR"
                     /locus_tag="OO572_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6660963.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            18177..19532
                     /gene="melA"
                     /locus_tag="OO572_001946"
     CDS             18177..19532
                     /gene="melA"
                     /locus_tag="OO572_001946"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6660964.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            19635..21056
                     /gene="melB"
                     /locus_tag="OO572_001947"
     CDS             19635..21056
                     /gene="melB"
                     /locus_tag="OO572_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6660965.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(21195..21824)
                     /locus_tag="OO572_001948"
     CDS             complement(21195..21824)
                     /locus_tag="OO572_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6660966.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(21947..23593)
                     /gene="fumB"
                     /locus_tag="OO572_001949"
     CDS             complement(21947..23593)
                     /gene="fumB"
                     /locus_tag="OO572_001949"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6660967.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(23671..25011)
                     /gene="dcuB"
                     /locus_tag="OO572_001950"
     CDS             complement(23671..25011)
                     /gene="dcuB"
                     /locus_tag="OO572_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6660968.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(25582..26301)
                     /gene="dcuR"
                     /locus_tag="OO572_001951"
     CDS             complement(25582..26301)
                     /gene="dcuR"
                     /locus_tag="OO572_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6660969.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(26298..>27893)
                     /gene="dcuS"
                     /locus_tag="OO572_001952"
     CDS             complement(26298..>27893)
                     /gene="dcuS"
                     /locus_tag="OO572_001952"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6660970.1"
                     /translation="RPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKA
                     LAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQ
                     RIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRV
                     TQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQS
                     IKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTP
                     RRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRE
                     RSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISK
                     INRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTL
                     HYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAV
                     ESEPGIFTQFFVQIPWDGERSNR"
BASE COUNT         6622 a         6832 c         7272 g         7167 t
ORIGIN      
        1 gctccggtct gtaggccgga taaggtgctt gcaccgcatc cggcatcaac gcctacacat
       61 tgtcggatgc gacgccgggg cgtcttatcc gacctacgat ttactgcacc gctttactca
      121 ccgaactcat cgcgcttaac gcgttgttca ggcggtccag gtcatccagc tgcgcctgaa
      181 tctcggtggt tttcgccagc ttgtcctgct cattcagccc tttattgcct ttcacgcttt
      241 gtatctcttt aaacagtgcg agctggcgaa tcggcaccag ttgcaggtcg ctggaagcgc
      301 ggaacggcgc ccagcccagg cgttccagca ccgctttctc ttccggcgtt tttccgtagt
      361 tcataaagaa gtcgtagatc ttgtctttgg tagtttcgga cagattcttg cgccagacga
      421 tcggatcgcc taggatcagc ggcgatttcc agatcacctt cagctcttta agcttctccg
      481 gcgcggaggt tttcagcttg tcgaggtttt cggtgttgtt ggtggcaaca tccacttgct
      541 tgttggcgac ggccagcgcg ttggtttcat gcccggcgtt gacggtgcgc ttgaagtcgc
      601 tggcggagat attgttttta gcgaagacgt agtaaccggg gacgaggaag ccagaggtgg
      661 agttaggatc gccattgccg aaggtgagat ctttccgctt cgccagcaga tcgttcaggt
      721 tgttgatcgg gctgtctttg ttgacgatca acacgctcca gtaacccggc gatccatccg
      781 ccgcgaccgt ctgggcgaag acctggccat tggcgcgatc caccgcttcc atcgccgaca
      841 ggttgccgta ccaggcaata tccactttat tgaagcgcat cccctggata atgcccgcgt
      901 agtccggggc aaagaaggcg ttgaccttca cgcccagttt cttctccata tcctgtaaaa
      961 acggcgtcca ttgcggtttc aggttttgct gtgattccgt tgaaataatg ccgaaattca
     1021 gcgccttttc ctgctcttcg gcgtgtgccg gggttaacag ggtgctgagg ctgaacatgc
     1081 tggtgaaggc cagcgaggca attatcttag cgttcattcg ttttcctcaa tgatggggat
     1141 atcaggcagc tttcgcgttc tcttcgatgc ggttaatgct gcggtagaga tggtcaaaac
     1201 gttcgttatc aaacagttgg ctgctgccgt cgtagaagac gtgcccctga cgcagggcga
     1261 cgatgcgttc gcagtagcgc agggcgtaat ccacctgatg cagcgtgacg accacggtga
     1321 tgccgtcgtt ctggttgatg tcgcgcaggg tgtccatcac gatgcgcgct gattccgggt
     1381 ccagcgaggc gatgggttca tcggccagaa tcactttcgc ctgctgcatc agcgcacggg
     1441 caatcgccac acgttgctgc tggccgccgg agagggtgga aacgcgctga tgggcaaaat
     1501 gcaccatgcc aacgcgggtc agcgcctgta acgcgcgctg tttctgctcg ccggtgaacc
     1561 agctaaaaca ggtgcgccag aacggcgtgc tgccgagcgc gccaatcagc acgttctcca
     1621 gtacgctcag gcggttcacc aggttgaatt gttgaaagat gtagccggtg ttggcgcggc
     1681 ttttgcggat atcgcgggcc aggcggcctt cgcgctggac agtgcggccc agtagctcga
     1741 tatggctacc ggcggattta tcgccggtaa tcaaaccgct taaatgacgt aaaagggtgg
     1801 atttgccgga acccgacggc ccaagcagag ccaccatttc actgtgatga atgttcagat
     1861 caaccgcatg cagcgcttga tgctgattga aggttttggc gagcttctcg acacggataa
     1921 tcgtttgcat gatgcagcct ccctaaaaaa gtggctccat cgtggcggat tattgtgacg
     1981 atttggttaa ttaaaagtga ctaacagatg aagagttaac gggaattcga tgacagtgcg
     2041 ggagggcggg cctcccggcg ggttattgcg ttggttgttg tttgacgaca ttaatcatcc
     2101 acggtacgcc aaatttatcg gtgactttgc caaagccatg cgcccagaaa gtttcctgcc
     2161 aggccatttc gatttttccg ttagcggcaa ggttatcaaa ccagcgtttt ccttcttcga
     2221 cctgttgcga atcgagcacc agcgtaaagc cggagtagct ggcttttcct gacggtatag
     2281 catcgctcat catgatgtcg cttccggcaa tgcgcacgtt ggcatgagcg atggcggtat
     2341 cgggaaattg cattccggaa gggcagttct cggcgctgtc ctgcgctgat ttcggcattt
     2401 cgccgaagct gattttatag agcagttccg cgcccaacgt acgttgataa taggcaatcg
     2461 cgtcggaaca gttaccggca aaagagaggt agggacttaa cggcatgatg gtgacctcag
     2521 ttaagagaag ccagttaagt gtagttcgaa atttatacag atgagaggct gcctgataag
     2581 acgcgcaagc gtcgcatcag gcagtcggca tcgttgccgg atgcggcgcg agcgccttat
     2641 ccggcttacc gatcacaata caatcagttc tttttcacaa actcagattt cagtttcatc
     2701 ggaccaaaac cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag gcggatgttt
     2761 ttcactttgg tgccaatttt cagcatcgaa gagctacctt tcaccttcag atctttaatg
     2821 atggtaacgc tgtcgccgtc agccagcaga ttgccgttag catctttaac gatcagctcg
     2881 tcgctttcct gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg gcagatgtac
     2941 atgccgttat cttcgtaagt gtattcggag ttgcattttg ggcagtgtgg taatgacatg
     3001 tggatttcct caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt gccgaaaaag
     3061 gccgcaatta tacacaaaat ccctgagttt gtcgggggta ctgctgattt tagacgacga
     3121 tcgttttcat ttgaaaggaa acgcaaaaag aaaataatct ttttgcctct atttattatt
     3181 taagataatg ttaatgctct acttgttctt tttttattca catgatattt ttatctttct
     3241 gacgttttaa acgcatcaga agacgttatt tattctgata aaaaaacaac acactaaata
     3301 taaaattatt tcttgcagta attataaaag ccgaaaacag aactcaaaaa caatctggct
     3361 aaataaaagt aacaaaattt gctttaagga agaattttct atgtacacac agaccctgta
     3421 tgagttaagt caggaggctg aacgcctgtt acagctttct cgccaacagt tgcagttact
     3481 ggaaaaaatg cctctctctg tacccggaga cgacgcgcca caactggctt taccctggag
     3541 tcagcctaat atcgccgaac gtcacgcgat gctgaataat gagttgtgta aaatttcccg
     3601 actggaaatg gtgctggcaa ttgtcggtac catgaaagca gggaaatcaa ccaccattaa
     3661 tgccattgtt ggtacggagg ttctgcctaa tcgtaatcgc ccaatgactg cgctgccgac
     3721 gcttattcgc catacgcccg ggcaaaagga accggtactg catttttcac atgtcgcgcc
     3781 aatcgattgt ttaattcaac aattacaaca gcgcctgcgt gattgcgata ttaagcatct
     3841 gaccgatgtg ctggaaatag ataaagatat gcgtgcgctt atgcagcgga tcgaaaatgg
     3901 cgtcgctttc gaaaaatatt atctgggtgc ccagcctatt tttcattgtc tgaaaagttt
     3961 gaatgattta gtgcgactgg cgaaggcgct ggacgtcgat tttccttttt ctgcttacgc
     4021 cgccattgag catattcccg tgattgaagt ggagtttgtc catctggcgg ggctggaaag
     4081 ttatcccggt cagttgacgt tactggatac ccccgggcca aatgaagccg ggcaaccgca
     4141 tctgcaaaaa atgcttaacc agcagctggc acgcgcctcg gcggtactgg cggtgctgga
     4201 ttatacgcaa ctgaaatcga tctccgatga agaggtccgt gaggcgattt tggcggtggg
     4261 gcaatcggtg ccgctgtatg tgctggtcaa taagttcgat caacaggatc gtaacagtga
     4321 cgacgccgac caggtgcggg cactgatttc cgggacgctg atgaaaggct gtattacgcc
     4381 acagcagata ttcccggtgt cgtcgatgtg gggctacctg gcgaatcggg cgcgctatga
     4441 gttagccaac aacggtaagt taccagcgcc agagcaacaa cgctgggtgg aagattttgc
     4501 ccatgccgcg ctcggcaggc gctggcgtca tgccgatctg gcggacctcg aacatattcg
     4561 tcatgctgcc gatcagttgt gggaagattc gctgttcgcc cagccaattc aggcgttgct
     4621 tcatgccgct tacgctaacg cctcattgta tgctctgcga tctgctgcgc ataaactgtt
     4681 gaattacgcg cagcaggcgc gggaatacct ggattttcgt gcgcacgggt taaacgtcgc
     4741 ttgtgaacaa ttgcggcaaa atatccatca ggtcgaagaa agtttgcagc tattgcaact
     4801 caatcaggcg caggtgagcg gcgagattaa acatgaaatc gagctggccc tgacctccgc
     4861 caaccacttt ctgcgtcaac agcaagatgc ggtgaatgcg cagttagccg ccttgtttca
     4921 ggatgattcg gagccgttaa gcgagattcg tacccgctgt gagacactgt tacagacggc
     4981 gcagaacacc atcagtcgcg actttacgct gcgttttgcc gagatggaat ccaccctttg
     5041 ccgggtgtta accgatgtta ttcgccccat tgagcaacaa gtcaaaatgg aattgagcga
     5101 gtcagggttt cgtcctgggt ttcattttcc tgtttttcac ggcgtagttc cccacttcaa
     5161 cactcgccag ctgttcagtg aagtcatttc gcgccaggaa gcaacggacg agcagagcac
     5221 gcgtttaggc gttgtgcgtg agactttttc gcgctggttg aatcagcccg actggggacg
     5281 gggaaatgag aaatccccga cagaaacggt tgattacagt gtgttgcaac gagcattaag
     5341 cgcagaagtc gatctctatt gccaacaaat ggctaaagtt ctggcagagc aggtcgatga
     5401 atctgttacg gcaggcatga atactttttt cgctgagttc gcttcatgtt tgacagaatt
     5461 acagacgcgt ttacgcgaaa gtctggctct gcgtcaacaa aatgaatcgg tggtcaggct
     5521 gatgcagcag caattgcagc agactgtgat gactcacagc tggatttaca ccgacgctca
     5581 gctgttacgc gatgatattc aaacactttt cacggcagaa cgatattgac caagacgtta
     5641 ctggacggcc ccggtcgcgt gctggagtcg gttcatccgc gctttttagt ggatctggcg
     5701 cagggcgatg atgcccgcca tcctcaagcc catcagcagc agtttcgtga acgactgatg
     5761 caggaacttc tttcgcgtgt gcagcttcag acatggacga acggcggcat gttaaatgcg
     5821 ccgcttagcc tgcgactgac aatggtggaa aaactggcgt cgatgctgga tcccggtcat
     5881 ctggcgctga cgcagatcgc gcagcatctg gcgctgctgc aaaaaatgga tcaccgccag
     5941 cactctgctt tcccggagct cccccagcaa attgccgcct tgtatgagtg gttttcagcc
     6001 cgttgtcgct ggaaggaaaa ggcgttaacg caacgaggcc tactggtgca ggcaggtgat
     6061 cagagcgagc aaatttttac ccgctggcgt gctggggcgt ataacgcctg gtcgttgcct
     6121 gggcgctgtt ttatcgttct ggaggagttg cgctggggtg catttggcga tgcctgccgt
     6181 ctgggaagcc cgcaagcggt ggtgttgttg ctgggtgatt tgcgcgagaa agcgacacaa
     6241 catctggcag agagtatcaa tgcggcaccg actacgcgtc actattacca tcagtggttt
     6301 gcctcttcga ccgttccgac gggcggggat catgctgatt ttttaagttg gctgggaaag
     6361 tggaccacgg cagataaaca acccgtttgc tggtcagtga cccaacgctg gcaaactgtc
     6421 gcgctgggga tgccacgact ctgttcagcg cagcgtttgg tgggggcaat ggtcgaggaa
     6481 atcttctctg taaatttggc gtaaataatc agttacatca atgagtccta aacgaaatcc
     6541 atgtgtgaag ttgatcacaa atttaaacac tggtagggta aaaaggtcat taactgccca
     6601 attcaggcgt caactggttt gattgtacat tccttaaccg gagggtgtaa gcaaacccgc
     6661 tacgcttgtt acagagattg catcctgcaa ttcccgctcc ccttttgcgg ccgtcgcgct
     6721 gatttttctg gcgtttgcgg aaatgggcca actctgcgag gaaagctatg ctgaaaagga
     6781 aaaaagtaaa accgattacc cttcgtgatg tcaccattat tgatgacggt aaactgcgta
     6841 aagccattac cgcagcatca ctgggtaatg caatggaatg gtttgatttt ggtgtttatg
     6901 gttttgttgc ttacgcatta ggtaaagttt ttttcccggg ggctgacccc agcgtgcaga
     6961 tggttgctgc acttgccact ttctccgttc cctttctgat tcgaccgctt ggcggactct
     7021 tctttggtat gttgggcgat aaatatggtc gccagaagat cctcgctatc actattgtga
     7081 ttatgtcgat cagtacgttc tgtattggct taataccgtc ctacgacacg attggtattt
     7141 gggcaccgat tctgctgttg atctgtaaga tggcacaagg tttctcggtc ggtggtgaat
     7201 ataccggggc gtcgatattt gttgcggaat actcccctga ccgtaaacgt ggctttatgg
     7261 gcagctggct ggacttcggt tctattgccg ggtttgtgct gggtgcgggc gtggtggtgt
     7321 taatttcgac cattgtcggc gaagcgaact tcctcgactg gggctggcgt attccgttct
     7381 ttattgctct gccgttaggg attatcgggc tttacctgcg ccatgcgctg gaagaaactc
     7441 cggcgttcca gcagcatgtt gataaactgg aacagggcga ccgcgaaggt ttgcaggatg
     7501 gcccgaaagt ctcgtttaaa gagattgcca ctaaatactg gcgcagcctg ttgacatgta
     7561 ttggtctggt aattgccacc aacgtgactt actacatgtt gctgacctat atgccgagtt
     7621 atttgtcgca taacctgcat tactccgaag accacggggt gctgattatt atcgccatta
     7681 tgatcggtat gctgtttgtc cagccggtga tgggcttgct gagtgaccgt tttggccgtc
     7741 gtccgtttgt gctacttggt agtgttgcac tgtttgtgtt ggcgatcccg gcgtttattc
     7801 tgattaacag taacgtcatc ggcctgattt ttgccgggtt actgatgctg gcggtgatcc
     7861 ttaactgctt tatgggcgtt atggcttcta ccttgccagc gatgttcccg acgcatatcc
     7921 gttacagcgc gctggcggcg gcatttaata tttcggtgct ggttgccggt ctgacgccaa
     7981 cactggcggc ctggctggtc gaaagctcgc agaatctgat gatgcctgcc tattacctga
     8041 tggtagtggc ggtgattggt ttaatcaccg gcgtaaccat gaaagagacg gcaaatcgtc
     8101 cgttgaaagg tgcgacaccg gcggcgtcag atatacagga agcgaaggaa attctcgtcg
     8161 agcattacga taatatcgag cagaaaatcg ataatattga ccacgagatt gccgatttgc
     8221 aggcgaaacg tacccgcctg gtgcagcaac atccgcgaat tgatgaataa gctgaaacgg
     8281 atggcctgat gtgacgctgt cttatcaggc caattgaact cttaaggttc acttaatctc
     8341 tgacgcgcat actctcctcc aggttaacgg aggagagtgc aatgaaaaac cgtgtttatg
     8401 aaagtttaac taccgtgttc agcgtgctgg tggtcagcag ctttctttat atctggtttg
     8461 ccacgttctg atctttcttc agccgtaccc aggcccgcgt gccggaagtc tcttgccggt
     8521 tttgcaggaa aaactgcccg tgatgcaact gtgtaatgcg gctgacaata cttaacccca
     8581 gaccaatccc gccataacgg ctgtccatac gtacaaacgc tttactcaac tccccgcatt
     8641 tactctcatc aatacctggt ccttcatctt caactgccat gaccgctccg ccatcttctt
     8701 gcagcttaat cataatgttg ctgccttgcg ggctgtaacg atgggcgttt tctaccaggt
     8761 ttcgcaataa catccgcagc agggttgcat caccctgaac ggtgatgtcg gcggcgctct
     8821 ctggcaatag cagggtttgc tgtcgctggt cgagcatggt actgagttcg tcatacgagg
     8881 ggagaatgac atcttccagc agttttacat gttgataatt accggaagaa aatgactgtc
     8941 cggcacgcgc cagttgcagc agctgggaga cgctctccat catctgatca agccgtgcca
     9001 ctaacggtgc tacatcaatg tgatgcgttt tcgccagcag ttccagatgc aaacgcaccc
     9061 ccgccagtgg cgttcgcagt tcgtgcgcga cgtcagcggt aaacaacctt tcgttatcca
     9121 gcgtgctggt caggcgactg accagatcgt tcaacgccga aaccaccgct tcgatttcga
     9181 gggtggcgct gtgaatggca atgggcgtta agttgtcggc ggtgcgcgct tccagctctt
     9241 tttgcagctc cgccagcggg cgggtgatgc ggcgtaccgc ctgatagcag ataaatagcg
     9301 tcaggctgac cataaagacg ccggggacaa tcaggctggc gaccgcctcg cggatctcac
     9361 gcatgatgtg gcgatcgttg ttgcgattgt cgcgtagcgc ctgctcaaac agctgaatct
     9421 gctcggtact ttcatgccat agccagaaga cgctgatcag ctcaaacacc aacaaaatgg
     9481 ccccgatggt caatatcagc cgttggcgca gcgatatagg tcggcgcaga aaacgcatca
     9541 gattcaatta gttttcctca ttcgcgacca gcatatagcc aaagccgcgc acggtgcgga
     9601 tacgggcttt gcccactttg tcgcgcagat tgtggatatg cacttccagg gtgttggtcg
     9661 agggttcatt gtcccagtta tagatgtcgt tgtagagaat ttcccgatgt accggactgc
     9721 ctgctttgag cattaaccgt gacagcagag catattcttt gggcgtcaga atcaactctt
     9781 caccgcccat ccatacctga cggcgaccca tattcagcgt cagattgcca acaatcagct
     9841 cactttcgcc ctgattatta tggcgtcgta gcagggcgcg gatacgggcg tgtaactctt
     9901 ccagcgcaaa aggcttcacc agatagtcat cggcaccgac atccagcccg gcgattttgt
     9961 cggtcagcgt atcgcgagcg gtgaggatca gtaccggcag ggtgtatttt ttctgccgga
    10021 tacgggcgag aaaatgcagt ccatcttcgt cgggtaaccc taaatccagt accaccaggc
    10081 tgtaatgacc gtcctcaagg ctttgttccg ccatccgcgc ggttgtcacg ccatcgcacg
    10141 cgtagccttc ggtttgcgcc gccagaatca gtccctgcaa taacagcgta tcgtcttcaa
    10201 caatcagaat tttcatttac tcactctcct gcaagtttgc agaatatcat ccgcggcctg
    10261 gtaatacttc gtctcaacgc cagttaatcc caatagcgtg gagaataaat tgtcttgtga
    10321 atagtgttgc gtttgcgcct gtttttgcag gcagttctgg tcaacctgat accgcttttg
    10381 ataatcctcc gacagccaca gcagcattgg cacctgtttt tggctatccg gggcgatggc
    10441 ataaggcaga ccgtgcagat agatgccatt ttcacctaac gattcaccgt ggtcagaaag
    10501 ataaaccagg ctggtggtaa atttatcctg atgttcttta agcagattaa tcgctttatc
    10561 aacaatatag tcgacgtaaa ccagcgtgtt gtcgtaagtg ttcaccagtt gctctttggt
    10621 acaggtctgg atctcattgg tgtcgcaggt tggggtaaat ttcctgaact gcggcggata
    10681 gcggttgtaa taggtcggac cgtggctgcc gatggtgtgt aagacaatca cgccatcacc
    10741 ctgcaggtta ttgatgtact cttccagccc gtggaacagc acttcgtcat agcattcgcc
    10801 gttgatgcac tgatcaggca gattcagcgc ggtgacgttc tggtgaggca cgcggtcgca
    10861 ggcaccttta cagccgccat cgttgtcatt ccacagcacg ttgatgcccg ctcgctgaat
    10921 gatatccagc acgccttcct ggtgctgtgc cagctcttct ttgtagtgct cacgcggcat
    10981 atccgagaac atgcacggta ctgaaactgc cgttgccgtg ccgcaagatg cggtattagg
    11041 gaaatagacc acgttatctt tcgccagccg cgggttagtt tcacgcgggt agccgttgag
    11101 ggagaagttc tccgcccgcg aggtttcgcc gacaatcagg atggtcaaat ttttacgttt
    11161 ttcgttctgc attaacgggt tgcggtgcgc gtcttcacca attcgcacca gcggcagatt
    11221 tgccagtcgc tgatgggagt accatgacca gctggcaaca atgctgttag aggggcttaa
    11281 ggatttcacc agctctttgt tattgcggaa caacgaggcg tagtctttat aaaacagtgc
    11341 ggcgaccagc aaaatcagca gtacagaaac cagaatattg gctccacgga aaagaacact
    11401 gcgcagacgc gaggtggcag gtttgatttt tagccagcag gcaatcagct cagcaagcac
    11461 gccgctgaat cccagcgtta ataacatttg cggtgtcatc agcgcataac tttctgctgg
    11521 agtggtatca ataatattgg caatcatcga gcggtcgatg acgatgccgt aagtcattat
    11581 gaaatattgt gcagccgcgc caatcagaat aaacaggcag gccagtggtc gattaagcca
    11641 taagaaagag cttagtgtca ggacaatatt aatcacgctg aaagcgacaa ccggcatcga
    11701 caagaaaacc agtacgttat gcagcgaatc cagcggcagc gcctgcaaca cctgtttaaa
    11761 aaaggcaata ttcaggcaga tagagatata aaaagcggcc aacaatagcc aggcgagtaa
    11821 attcaaagag ggtcttttta gtaggcgctt caacatcacg gtgtttccat cgaacaaagt
    11881 gcgcatatgc tcgcaaagca aaattaagcc aaccttaagt tcttaaggtt ggcttttatg
    11941 tttgctggat taaggggaat taatctttgc ttattggtgc atctaaggga tacgggtttt
    12001 tatgtagccg gttgtaattc agcgcataca tggcggtgat gaccatcagg gtgacaaatg
    12061 accacataac ctctttcgcc ccggacccca ccacggccca aatgcagtag aggaaggcaa
    12121 tggtagtaac agccagatat gccgggcgtg ctttaccaaa gtgaccgtgg ccgagcagca
    12181 gtaacgccgc acaggtgtaa agatatggca ccagtgtaaa gatgaccgag acggaagaaa
    12241 ccagaccgaa ctctttggtc gcgtttggtg aaatgctgct gagctggaag atggtcatca
    12301 aaataccgac gataatcagc cccgccactg gcgtacccgc tttatttaca cgggcaaaaa
    12361 tcggtgggaa cagtccgtca tcggcagcgg ctttcgccgt ttgacccgcc agcaacgtcc
    12421 agccgcccag tgaacctaag caacccgcag ctgcgcagaa ggaaacaatg gccccagcgg
    12481 tgtcacccaa cgccatccgt gcggcatcac cgaatggcga ggcagaaacg cgcagtgcgg
    12541 cattagggat catccccata atcgcggtgg tggagagcac atagcaaacg gcggcaatca
    12601 ataccccacc aatggtggcg atagggacat tgcgtttcgg gtttttcacc acacctgcgg
    12661 caacggaggc actttccaca ccgatgaacg accacagcgt aacgttaagg gtactttgaa
    12721 tcgcaccgaa ggtgcccagg ccgctgacgt tccatgccgc catataggtt tcaccacgga
    12781 accagaacca gccaaatacg gcaatcccga cgatggggat cagcgccagc acagtggcaa
    12841 cggcctgcac gcgggtgatc attttcggac cgacaatgtt cagcaggacg aagatccaca
    12901 gcaccacgac gcaggtgatg gttaatacca atggatcttt cagaatcggg aagaagtaac
    12961 ttaaatatcc tacgccaatg accaccatgg cgatattgcc gatccagcag gccagccagt
    13021 agaggacgtt ggtttgataa ccgagaaacg ggccaaagca gcggcgggcg taagcgtaag
    13081 aaccaccagg acttgggtcg aggaacgaca ttttggcgta taccatcgag agccccagcg
    13141 caccgataat cgtcaccaac catccataaa tggcaatccc gccagtggag gccaggtttg
    13201 caggtaacag aaaaacacct gaccccataa tattccccga caccatcagg gtgacgggga
    13261 ttaagcccac tttgtgagca tcagcatccg aagacataat taaactcctg cgaaggcgag
    13321 cttcgtgaca ataaattacg tcatatcata cgcctgcatt cgatagttga attattatcg
    13381 accgggttat cgctgatgcc gttattaata tagctcttcg ttaaggttaa aaatcaggcg
    13441 acagtgcgtt ctctgtgctg actaacataa tgcagcggcg tcataccgta gaagtcttta
    13501 aagacagaaa taaagtacga cgtactgttg tagccgcagg actgtgatac ctgagagata
    13561 tttttaccgt ccatcattaa ttcatttacg gcataacgca tgcggcaggt ggtgattatt
    13621 tggctataac tggtgttttc gcttttcaac tttttcttta acagacttgg gctaagacat
    13681 aaacaactgg ctaccatact aagattccag tctttgtgaa tatcgctttc aataatttga
    13741 taaacgctgt cacttacaca attacgtaac atatacatca ttaatgaaag gaatttttta
    13801 ctgtcgagaa aacgggatag caccgtgaat aataatgcgc gtgttcgttc cttttctgac
    13861 tcagtttctg cgggcatcat gctgtgttgc gcggcaacgc gaaagacgtc tggcgtcagg
    13921 catggcaggg tgagtatggg cgtagcgcta cgttgccata ctggtatttg cgagagatct
    13981 ttattcagaa accggaggta atctttgatg atgtcgtgac taatatgagc aaccaaagtg
    14041 ttgttaagag aggagacgtc gataatatta ttttcacaat ttaataacgc catatggtta
    14101 gctttaaggc taataggttc tttcccattc acccttatcc agacatcttt ttcagtcaat
    14161 aaaacaatac aaggttggtc gctgcaaatc ctcatacatg tactcctgag tgcgaataaa
    14221 agaccagttc tcgctaaagc aaagcgatac cgggaaatgg ttatataatt gcatgatgaa
    14281 tataaacatg cataaaaata acgagtttta ctaatattac aaatttttat agtaatttct
    14341 catgtacata tttttttaca ggataaataa caggcaaaaa aaagcgtggg gtggggccgc
    14401 gcaaaaagta tgacattttt gtttgcagca atttttatga aaacgttgcc atactcaaaa
    14461 ggcctaaggc caggcggcgt aatgttattt aaacaattac gccttcagcg gaatagtggt
    14521 tacgctttca cgcacataac gtggtaaata ccgtcaataa tttcagtccc ttcagtttcg
    14581 tgttcaaatc tagggaaatg gtggtcccag gattgcagcg agcgtaaata acttacttgc
    14641 ggactgtttt tatcgccgaa gttttcacca gacagcagca tcgggattcc tggcggatac
    14701 gggataactg agtttgccgc gatgcgtcct ggcagatttt caatggatac cagttcgaca
    14761 ttgttgtcga caatcgcgtt gtacgcttca cgcggggtga tttccgccac cggcaggccg
    14821 gaataggctt cgttcaaccg tgcgccaggg ttgttttctt tcagccaggc aaacatggtg
    14881 tcacccagat cgtgaatccc catgttcgcg taagtgtcag gatattgttc aacaagttcc
    14941 ggcatcacct gcgccagcgg tgtgttggcg tcatagtggt gtttgaagga gcaaagggtg
    15001 ttaaccagag tcccccattt cccacgggtt acgcccatag agaacaggaa cataatttgg
    15061 aagtcagtgg tgcgggtagg cacaatgccg tggcgaccaa gccaggcagt gaccagcgcc
    15121 gccggaacac cggtttcttc cagttcacca tcttcaccca ttcccggagc aaggatgctg
    15181 actttaattg ggtcgagcat actccagtta tccggaatat ctttgaagcc gtgccagctt
    15241 tcgcccggat gcattaccca gcagtcctga acggtggtca gcagtttggt tggtgcgtca
    15301 gcaaagtcat aggttttgcc ggtttgtggg tcggtgacga cttctttgtt ccacggtttg
    15361 aagaaccagc taccgtcagc ggtgaactct ttatatagcc gcgccatcgc ctgacggaaa
    15421 tcaaccgctt cgtcaatcac ttcctgtgtc agtgacaggc cgctgttgcc gtccatcatc
    15481 gacaccgcca cgtcgttgga tgcgcagatg gcatacagcg gggaggtggt ggcatgcatc
    15541 atgtaggcct ggttgaagcg ggagaagtta atcgccccac gaccttcacg tacatgaata
    15601 taagaagcct gtgacagcgc attcagcagt ttgtggatgg agtgggtggc gaaaacggta
    15661 ggaccgttgt gatcgccagg ttcgccgcgc atggcatagt gatcggcata gatcgggttg
    15721 aaacgtgcat agccgtacca ggcttcgtca aagtgcagac gatcggaggt tttttccagc
    15781 agatcctgcg cttctttagc gttataacac acgccgtcat aggtgcagtt ggttaccacg
    15841 cagtaagacg gtttttgccc ggctttgtct ttggtcagcg ggctttcact gattttcttt
    15901 tgcaaggttt caggttgcat ttcctgcgga tagattggcc cgataatgcc gtagcggttg
    15961 cggcttggca ccatatagac cggtttcgcg cctgtcagca tcaaaccttg ttcgatggat
    16021 ttatggcagt tacggtcaac gaccacgaca tcgttatcgg tcatgcaagc ctgcatgatg
    16081 gtgcggttag agccggaagt accgacgact accgaccagg agcgatcggc accaaatacg
    16141 cgtgcggcat atttttcgct ttcgccaaat gcgccagtat ggtcaagcaa agaaccgagg
    16201 gaagttcgtt cgatgcccat gtcggtacgg aacagatttt caccatagta gtcatggtag
    16261 aaacgtccgg cgggtgtttt ggtaaaacca acgccgccct ggtggcctgg cgctgcccag
    16321 gaatattcat ggatgtcact atatttcatc agcgcgctga acagtggcgg caacagctgc
    16381 tggcggtagc gggtcatcgc ggcaacggcg cgtccggcga taaagtcggc ggtatcttcc
    16441 agaatccagg cgaattcatc gacaagctcc agcagatccc ggtccattgc ggcgagggct
    16501 ttttcccgat cgcccaacag gaagaccggc acgttttgtt ggcgctcatg aagcttaccg
    16561 atcaattgtc tgacgttttg atgttcgtcc ggatgctcca tttgatagct gaacatcagg
    16621 cagtcaatgg cttcgtttga agagagaatg gcaaaaccat catcaaagga ggtggattta
    16681 atcacggtaa cattttgctg gcttaaagca tctgccagac gctcaacggc gttaccgacc
    16741 caggtgtctt gatggagaaa ctcgctttca acaattaata ctttcatcat tgcttacccg
    16801 gttatgaagg aatcttcgtt gcgggcaagt atcttccggc cttttcgggc tgtaaagcgc
    16861 gtgaaaaaca aacatttttg aaatatttag ttttataaac aatacgttat gtgattattt
    16921 taaattattt tcactgctta ttatcgacgg ctaaactatt tttttggctg atactgatat
    16981 cgtctttagc cgggaaacgt ctggcggcgc tgttggctaa gtttgcggta ttgttgcggc
    17041 gacatgccga catatttgcc gaacgtgctg taaaaacgac tacttgaacg aaagcctgcc
    17101 gtcagggcaa tatcgagaat acttttatcg gtatcgctca gtaacgcgcg aacgtggttg
    17161 atgcgcatcg cggtaatgta ctgtttcatc gtcaattgca tgacccgctg gaatatcccc
    17221 attgcatagt tggcgttaag tttgacgtgc tcagccacat cgttgatggt cagcgcctga
    17281 tcatagtttt cggcaataaa gcccagcatc tggctaacat aaaattgcgc atggcgcgag
    17341 acgctgtttt tgtgtgtgcg cgaggtttta ttgaccagaa tcggttccca gctagagagg
    17401 ctaaatcgct tgagcatcag gccaatttca tcaatggcga gctggcgaat ttgctcattc
    17461 ggactgttta attcctgctg ccagcggcgt acttcaaacg ggctaagttg ctgtgtggca
    17521 agtgatttga tcaccatgcc gtgagtgacg tggttaatca ggtctttatc cagcggccag
    17581 gagagaaaca gatgcatcgg cagattaaaa atcgccatgc tctgacaggt tccggtatct
    17641 gttagttggt gcggtgtaca ggcccagaac agcgtgatat gaccctgatt gatattcact
    17701 ttttcattgt tgatcaggta ttccacatcg ccatcgaaag gcacattcac ttcgacctga
    17761 ccatgccagt ggctggtggg catgatatgc ggtgcgcgaa actcaatctc catccgctgg
    17821 tattccgaat acagcgacag cgggctgcgg gtctgttttt cgtcgctgct gcacataaac
    17881 gtatctgtat tcatggatgg ctctctttcc tagaatatca gaattatggc aggagtgagg
    17941 gaggatgact gcgagtggga gcacggtttt caccctcttc ccagaggggc gaggggactg
    18001 tccgagcatc atgaggccga aaactctact tttcaggtaa tttattccca taaactcaga
    18061 tttactgctg cttcacgcag gatctgagtt tatgggaatg ctcaacctgg aagccggagg
    18121 ttttctgcag attcgcctgc catgatgaag ttattcaagc aagccaggag atctgcatga
    18181 tgtctgcacc caaaattaca tttatcggcg ctggttcgac gattttcgtt aaaaatattc
    18241 ttggtgatgt gttccatcgc gaggcgctga aaacggcgca tattgccctg atggacattg
    18301 accccacccg cctggaagag tcgcatattg tggtgcgtaa gctgatggat tcagcagggg
    18361 ccagcggcaa aatcacctgc cacacccaac agaaagaagc cttagaggat gccgattttg
    18421 tcgtggtggc atttcagatt ggcggttatg aaccttgcac ggtgactgat ttcgaggtct
    18481 gtaagcggca tggtctggaa caaaccattg ccgatacgtt ggggccgggc ggtattatgc
    18541 gcgcgctacg taccattccg catctgtggc aaatttgcga gaacatgacg gaagtctgcc
    18601 ccgatgccac catgctcaac tatgttaacc caatggcgat gaatacctgg gcgatgtatg
    18661 cccgctatcc gtatatcaaa caggtcgggc tgtgccattc ggtgcaggga acggcggaag
    18721 agctggcgcg tgacctcaat atcgacccgg ctacgctgcg ttaccgttgc gcaggtatca
    18781 accatatggc gttttacctg gagctggagc gcaaaaccgc cgacggcagt tacgtgaatc
    18841 tctacccgga actgctggcg gcttatgaag cagggcaggc accgaagcca aatattcatg
    18901 gtaatactcg ctgccagaat attgtgcgct acgaaatgtt caaaaagctg ggctacttcg
    18961 tcacggaatc atcagaacat tttgccgaat atacgccgtg gtttattaag ctaggtcgtg
    19021 aggatttgat tgagcgttat aaagtaccgc tggatgagta cccgaaacgc tgcgtcgagc
    19081 agctggcgaa ctggcataaa gagctggagg agtataaaaa cgcctcccgg attgatatta
    19141 aaccgtcacg ggaatatgcc agcacaatca tgaacgctat ctggactggc gagccgagtg
    19201 tgatttacgg caacgtccgt aacgatggtt tgattgataa cctgccacaa ggatgttgcg
    19261 tggaagtagc ctgtctggtt gatgctaatg gcattcagcc gaccaaagtc ggtacgctac
    19321 cttcgcatct ggccgccctg atgcaaacca acatcaacgt acagacgctg ctgaccgaag
    19381 ctattcttac ggaaaatcgc gaccgtgttt accacgccgc gatgatggac ccgcatactg
    19441 ccgccgtgct gggcattgac gaaatatatg ctcttgttga cgacctgatt gccgcccacg
    19501 gcgactggct gccaagctgg ttgcaccgtt aaaacgcgac taaacgctac tgcgccgggg
    19561 gatttattcc ggcgcacacc tctgacgata ccaataacag aaggcgggcg ttggtaacag
    19621 cgacccgata ccctatgagc atttcaatga ctacaaaact cagttatgga tttggagcgt
    19681 tcgggaagga ttttgcgatc ggcattgtgt atatgtacct catgtattac tacaccgatg
    19741 tcgtcgggct gtctgtgggt ttggtcggta ctttgtttct ggtggcgagg atctgggatg
    19801 ctattaacga tccgattatg ggatggattg taaatgctac gcgatcgcga tggggtaagt
    19861 tcaaaccctg gatcctgatc ggtacgttgg caaactctgt aatcttattt ctcctcttta
    19921 gtgcgcatct gtttgaaggt actactcaga ttgtctttgt ttgcgtgacc tacatcctct
    19981 ggggcatgac ttacaccatt atggatattc ccttctggtc gctggttcca accatcacgc
    20041 tcgataaacg tgaacgcgaa caactggttc cttatccgcg tttttttgcc agtctggcag
    20101 gctttgttac ggcaggtgtg acgctaccat ttgttaatta tgtcggcggt ggcgatcggg
    20161 gatttggctt tcagatgttc actctggtat tgatcgcctt ttttattgtt tcaaccatca
    20221 tcactctgcg caatgtgcat gaagtctttt cgtcagacaa tcaatcgtct gctgaaggaa
    20281 gccatctgac acttaaagcc atcgttgcgc ttatttataa aaacgatcag ctttcatgcc
    20341 tcttgggtat ggctcttgct tataatgtag ccagcaacat tattaccgga tttgctatct
    20401 attatttctc atatgttatc ggtgatgcgg atttgttccc ctattatctg tcgtatgcgg
    20461 gagctgctaa cctggtgacg ttagtattct tcccacgctt agttaaatca ttatcccgac
    20521 gcattttatg ggccggagca tctattcttc cggtgttaag ctgtggtgtt ctcctgttaa
    20581 tggcattaat gagctatcac aacgtcgtcc tcattgtgat tgcgggtatt ttgctgaatg
    20641 tgggaacggc gcttttctgg gtattacagg tcatcatggt ggcagatacc gttgattacg
    20701 gtgaatataa actgcacgta cgctgtgaaa gcatcgctta ctccgtgcag actatggtgg
    20761 tgaagggcgg ttcagccttt gcggcttttt tcattgcggt tgtgttaggg atgattggct
    20821 atgtaccgaa tgttgaacag tctacgcaag ccctattagg tatgcagttt attatgattg
    20881 ctctaccgac tctgtttttc atggtaacgc tgattctcta cttccgtttc tatcgcctca
    20941 atggcgacac gctgcgcagg atccagattc atctgctgga taaatatcgc aaagtaccgc
    21001 ccgagcctgt tcatgctgat attccggtcg gtgcagtgag tgatgtgaaa gcctgacgtg
    21061 gacaatagct aacgaaaatg gcctgatgcg atatgtttat caggccacgt cttaatgtaa
    21121 tgctttggat ttgcaaattt tgtagaccgg ataaggaatt cacgccgcat ccggcatcaa
    21181 caaagtgcac gttgttatcc agttatcaag ccatagcgcc gtagctggcg gcaatgaaag
    21241 cgagcgagca atatcactgt cgttaatgcg ccaagcagcg cgcagaacat atcggattgc
    21301 gtgtcccact ggtcgccctg ggtaccgaga aaatcatccg ctccctggcc catcgccagt
    21361 gccgcccacc actcgattaa ttcatacatc gcgcttatcg ccagcgccac gcaacagacc
    21421 agaaacgcca ccattttacg tccgcgcacg tacatcccgc gaacgagaat ttctcgtgcc
    21481 accagtgcag gcaccagccc ctggaaaaag tgccccagct tgtcatacgg attacgactc
    21541 aaccccagcc attcctgcac ctcaaaacca acggggactt tcgcgtaggt gtactgcccg
    21601 cctaccatca ggatgatggc gtgaaagaag atgagcgtat agagcagcag cgttaacggg
    21661 taacgtctgg cggtggcaag tagtagcggc acgacgataa tcaccggtgt cacttccagc
    21721 agccaggtga gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc
    21781 agtgcgccag tgttaagaat taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc
    21841 agggaaaata ctgactattc accagcaagc atgaaattac aatcgcaaaa gaggtggccc
    21901 agggggatca cctggcagca tgctgccagg cgctgggccg aagaggttac ttagtgcagt
    21961 tcgcgcactg tttgttgacg atttgctgga agaagtcgtt acctttgtca tcgaccagga
    22021 taaacgccgg gaaatcttct acttcgattt tccagatagc ttccataccc agctccggat
    22081 aagcgacgca ttccagatgc ttgatgctct gctgcgccag taccgccgcc ggaccgccga
    22141 tgctaccgag gtagaagccg ccgtgtttat gacacgcgtc ggtaacctgc tggctgcggt
    22201 tgcctttcgc cagcatgatc atgctgccgc cgtgggattg cagcagatcc acataggagt
    22261 ccatacgacc tgcggtggtt gggccaagtg aacctgatgg ataaccggca ggggttttcg
    22321 ccggacccgc gtagtagatc gggtgatctt tgatgtactg cggaagttct ttaccggcgt
    22381 caatcagctc tttcagcttg gcgtgtgcaa tatctcgacc cacaataatg gtgccagtga
    22441 gcgacaaacg agtggaaacc ggatattgcg aaagctgggc gaggatctct ttcatcgggc
    22501 ggttaaggtc gaccttcacc gcttcgcctt caccggcctg acgcagttct tgtggaatgt
    22561 actgacctag attgtgttcc agtttttcga tccagatacc ttcgcggttg attttcgctt
    22621 taatgttacg gtcagcggag caggagacgc ccatgcctac cgggcaggat gcgccgtgac
    22681 gtggcagacg gataacgcga atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa
    22741 gaccgagttt ctgggcctct tccagcagtt cctgttccag ctggacatcg cggaacgcct
    22801 gaccatgttc gttcccttcc gtcggcagtt catcgtaata gtgagcgctt gctaatttga
    22861 cggttttcag gttgttttcc gcagacgtac cgccaatcac aaacgcgata tggtacggcg
    22921 ggcaggctgc agtaccgagg gtacgcattt tctcgacgag gaagtttttc agtttgccgg
    22981 gagtcagcag ggctttggtt tcctggtaga gatacgtttt gttggcagag ccgccgcctt
    23041 tcgcaacgca aaggaatttg tactcatcgc catctaccgc gtacaggtcg atttgcgcag
    23101 gcaggttagt gccggtgttg acctctttgt acatgtccag cgccgcgttt tgcgagtagc
    23161 gcaggttatc ttcgatatag gtgttataga cgcctttcga cagcgcttct tcatcaccgc
    23221 caccggtcca cacgcgctgg ccttttttac cgacgatgat cgcggtgccg gtatcctggc
    23281 aggtcggcag cacgcctttg gcggcgattt cggagtttct taagaattgc agtgccacgt
    23341 acttgtcgtt ttcgctggct tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg
    23401 ccggacgtag catgaaagaa gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt
    23461 ctggttccac tttcaggatg gtttcgccgt cgaagtcggc aacgctaacg taatcggaag
    23521 tgagtagata gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg
    23581 gtttgtttga catagcttcc agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc
    23641 gcgtagtcac gggcggcacg caaagtgcat ttataagaac ccgtacatcg cggcgaagat
    23701 ccagccgaag acgcacgata cgctcacacc aatcaacccc ggcagaataa aactgtggtt
    23761 gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg ctgccagatc
    23821 gctcggataa gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta
    23881 tgccggatca acgccgatcg ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg
    23941 agagtttaca aacttggaaa ccagcagcag aacaatggca tacgcccacg gatactcttt
    24001 caccatttca cccagtacgc cctgaatttc agacatatgc gcaccgaaca tggtttctgc
    24061 catccatgcg ataccgtaca ccgccacgat ggcgatcata ccggaacgga agacttcgtt
    24121 ttttgagata gacgcgggat tggttttggt caggataata atcagcgccc cggtcagcag
    24181 cataaacatc tgaataacca gtaccatcga cagcggtttg ccgccgaagg atggacgcag
    24241 gtccgaatca gcaccaagaa gggcgactac agcgattgcc ccgaggaaaa tccacattgc
    24301 cagccagttg cttttcggca gttttttatc cagcagcgtc gcggtatcac cgtaaacata
    24361 ctcacggttt tccggtacgg agatgaattt ctggaactct tcgtctttat ccagatcttt
    24421 accgcggaac cagctgaaga taccgatcgc caggataccg attaacgtcg atggaatggt
    24481 gattgccagc agatcgagga actcaagatg gcgaccatca aaggtgacat tacccagcat
    24541 cgcaaccaga gacacgaccg caaccgacac cggactggcg ataatcccca tctgtgcacc
    24601 gatagaactt gccgccatcg gacgttccgg acggatgttg ttcttaatgg cgacgtcgta
    24661 gatgatcggc agaatggtgt aaaccacatg acccgtaccg caaagaatgg tcagtgtaca
    24721 ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg cggcgcagca gcttctcggc
    24781 aatttgcagc atgacatcaa gaccgcccga agcttgcaag gtcgccgatg ccgccaccac
    24841 cgcaatgata accagcatga catcaactgg tggtttacct ggctgaaggt ggaagacgaa
    24901 gaccagaatg accagaccga taccgcctaa taaacccagc gcgataccac cctttctggc
    24961 accataaaac agacatatta gtattatgat aagttggata gtaaataaca tgtgtgaacc
    25021 ctcgcgataa tcctatttaa atttttgctg aatagatcac agtcacgttc tgttttgtat
    25081 gaactgtttt cagagcctga ctaaatatcc gtttggtcgt taccggcttt agcaaatacc
    25141 tcacagtgaa tattggctga ttaaagcagc ggcaaattta tgcactgtga taagcggctt
    25201 tttcagagag aaaaactctc tggcgatggg tttatagttt gtactgcgca gatgttaatg
    25261 ttttgtttgc tgttatctta ttgatattta tcggtctatt ttttgttctc agggcagata
    25321 tcattaggtt tatagattaa tctgatctac ccatttgtgg gtaaaaatac acataatgcg
    25381 ggtgacataa tagttaatta acttttgtta tcgttttgaa attaaaaaca ccgttcatct
    25441 gaagagatat taatttttaa cgatgatgga gggataatta tatttgatct ggcacaagtt
    25501 ttactgatta acgatgtaac ttgtgccagg ggtattttgc attacggtaa taattattac
    25561 ttacagataa ccagcgatcc gttattggca atattgtttc agcagtgagt agtgttctgc
    25621 ctgaatacgg taacggtaaa ccggacgccc cgtgacgcca taatggatac tggtgaacaa
    25681 gatgtggcag ttgaccagcc agatgaggta ttttcggcag gaaacacgcg agatgttaac
    25741 ctcgttagcc agctcgtcgg ttgaaaactc atagtcctga tgcgcgtcaa tccactggca
    25801 cagcgtgcgt aacgtctgcg gcgttaagcc ttttggcaag cgacgaggat cctgttcgtt
    25861 ggagctgctg ccgtggatta gctgatcaag ctcggcctgg tcataatact gatgtttttc
    25921 cagcgccatt ttcttttgcc gccagccggt gagcgcctct tcaaagcggg aagcctggaa
    25981 gggtttgatc aggtaatcca cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc
    26041 ggctgcggag gagatgacaa tcacatcact tttgcaacgc gcgttatgca ggacaggcag
    26101 taaatcgagc ccgttctctt tttgcatata gatatcgagc aatatcaggt cgataggcgc
    26161 atcgctattg aagataatct ctttggcttt ctccagcgtc gaggctgttc cacagcattg
    26221 aaagcctggg atttgtgcta cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc
    26281 gataattaat acattgatca tctgttcgac ctctccccgt cccagggtat ctggacaaaa
    26341 aattgtgtga aaatccccgg ttccgattcc acggcgatgc tgccgccgag attttctacc
    26401 tgttgtttga caagtgctaa accgacgcct cgctcgcttc cttttgtcga gacacctttg
    26461 tcaaaaatgt gatcgatttt gtcgggtgcg atccccggtc catcatcgtt aacttcgcag
    26521 tgcagccagc cgtgacggta gtgcaatgtt acgctaattt cgccaccggg ttccggccct
    26581 aatgcttcca gcgcgttttc tatcagattt cccaatgtgg taatcagcgt cgcgacctgg
    26641 tcctcactgc cgctgtctgg cagctggctt tcactgttta aaatcagcgt atggcctaaa
    26701 tcggtcgcgc ggttaatctt gctgattaaa aaaccagcga taaccggaga tttgatctta
    26761 cccagcagag agccaatctc ttcctgatag ttattggctg ttttgagaat gtaatcttcc
    26821 aactgcttat aactcttcag atgcaataat ccgagaatca catgcaattt attcataaat
    26881 tcgtgggatc gttcacgaag tgcgtcagca tagttgacca gaccgtcgag tcgctgcatc
    26941 agtttacgaa cttcagtttt gtccctgaag gttgaaatgg caccgatgat aacgccatta
    27001 ctgcgcaccg gaacggtgtt gatcagtaat agccggtctt taatcgtaat ctcttcgtcg
    27061 cggcgcggcg taccgtcgcg taacacttcc gagacatcta ccacctgtga ccatgagtgg
    27121 ctgagcgtcg acagtttctc atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg
    27181 gcatcgttga tcagcgtgac ctcgccgcga tcgtccacgg caacgacgcc ttctttgata
    27241 gactgcaaca tggcctggcg ttgctcaaac agcgtggaga tttcgtaggg ttccaggccg
    27301 aaaaggattt ttttcagtac cttaaccaga atgcaggtgc caatcagtcc gaccagcatg
    27361 ccaaataata ccgaccagat aatgctccag cgactgtcat tgatctgttg ggtcacgcgg
    27421 cttaactcaa ggccgatcgc caccacgcca atttgtttat gattttcatc gtagatgggg
    27481 gtaaatacgc gtaaagcctg cgccagaaaa ccgcgattga tagcgacatt ttcttcgcca
    27541 ttcagcgcat taaggatgtc atcaccttta aatggctgac caatacgctg ggcttcagga
    27601 tgtgagtagc gaagactttg catatcggta acgacaataa acagcagatc gttgcgtttg
    27661 cgcacggctt ccgcgatggc ctggatgcca ctctcctgcg gttttttctg caagccctga
    27721 cggatttccg gcgagtcggc gagggtacgc gccactgcca gtgccttgtt ggccagcccg
    27781 tctcgcgtca tatcactgat ttgcgagaag taaatcagat gcaccaccaa tagcaccgag
    27841 aacagtaccg cgctgaccat taagatcaca gtggtactca atttcatcgg acg
//
LOCUS       DAJTYL010000047        27812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYL010000047 DAJTYL010000000
VERSION     DAJTYL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.047"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            159..929
                     /locus_tag="OO572_001953"
     CDS             159..929
                     /locus_tag="OO572_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6660971.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            920..1393
                     /locus_tag="OO572_001954"
     CDS             920..1393
                     /locus_tag="OO572_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6660972.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1500..2039
                     /gene="dnaT"
                     /locus_tag="OO572_001955"
     CDS             1500..2039
                     /gene="dnaT"
                     /locus_tag="OO572_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6660973.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2042..2779
                     /gene="dnaC"
                     /locus_tag="OO572_001956"
     CDS             2042..2779
                     /gene="dnaC"
                     /locus_tag="OO572_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6660974.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2828..3322
                     /locus_tag="OO572_001957"
     CDS             2828..3322
                     /locus_tag="OO572_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6660975.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3562..5853
                     /gene="opgB"
                     /locus_tag="OO572_001958"
     CDS             3562..5853
                     /gene="opgB"
                     /locus_tag="OO572_001958"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6660976.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5991..7013)
                     /gene="lgoD"
                     /locus_tag="OO572_001959"
                     /pseudo
     CDS             complement(5991..7013)
                     /gene="lgoD"
                     /locus_tag="OO572_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7152..8066
                     /locus_tag="OO572_001960"
     CDS             7152..8066
                     /locus_tag="OO572_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6660977.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8281..9642
                     /locus_tag="OO572_001961"
     CDS             8281..9642
                     /locus_tag="OO572_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6660978.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9691..11355)
                     /gene="tsr"
                     /locus_tag="OO572_001962"
     CDS             complement(9691..11355)
                     /gene="tsr"
                     /locus_tag="OO572_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6660979.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11474..11920)
                     /gene="hpaR"
                     /locus_tag="OO572_001963"
     CDS             complement(11474..11920)
                     /gene="hpaR"
                     /locus_tag="OO572_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6660980.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12192..13481
                     /locus_tag="OO572_001964"
     CDS             12192..13481
                     /locus_tag="OO572_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6660981.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13478..14944
                     /gene="hpaE"
                     /locus_tag="OO572_001965"
     CDS             13478..14944
                     /gene="hpaE"
                     /locus_tag="OO572_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6660982.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14946..15797
                     /gene="hpaD"
                     /locus_tag="OO572_001966"
     CDS             14946..15797
                     /gene="hpaD"
                     /locus_tag="OO572_001966"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6660983.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15807..16187
                     /locus_tag="OO572_001967"
     CDS             15807..16187
                     /locus_tag="OO572_001967"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6660984.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16255..17058
                     /gene="hpaH"
                     /locus_tag="OO572_001968"
     CDS             16255..17058
                     /gene="hpaH"
                     /locus_tag="OO572_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6660985.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17069..17872
                     /gene="hpaI"
                     /locus_tag="OO572_001969"
     CDS             17069..17872
                     /gene="hpaI"
                     /locus_tag="OO572_001969"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6660986.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17894..19249
                     /gene="hpaX"
                     /locus_tag="OO572_001970"
     CDS             17894..19249
                     /gene="hpaX"
                     /locus_tag="OO572_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6660987.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19259..20153
                     /gene="hpaA"
                     /locus_tag="OO572_001971"
                     /pseudo
     CDS             19259..20153
                     /gene="hpaA"
                     /locus_tag="OO572_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            20180..21117
                     /locus_tag="OO572_001972"
                     /pseudo
     CDS             20180..21117
                     /locus_tag="OO572_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21344..22902
                     /gene="hpaB"
                     /locus_tag="OO572_001973"
                     /pseudo
     CDS             21344..22902
                     /gene="hpaB"
                     /locus_tag="OO572_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22920..23432
                     /locus_tag="OO572_001974"
     CDS             22920..23432
                     /locus_tag="OO572_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6660988.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23825..25975
                     /gene="btsT"
                     /locus_tag="OO572_001975"
     CDS             23825..25975
                     /gene="btsT"
                     /locus_tag="OO572_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6660989.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26025..26228
                     /locus_tag="OO572_001976"
     CDS             26025..26228
                     /locus_tag="OO572_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6660990.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26239..27195
                     /gene="yjiA"
                     /locus_tag="OO572_001977"
     CDS             26239..27195
                     /gene="yjiA"
                     /locus_tag="OO572_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6660991.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27264..>27812)
                     /locus_tag="OO572_001978"
     CDS             complement(27264..>27812)
                     /locus_tag="OO572_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6660992.1"
                     /translation="QIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALD
                     EDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQ
                     PQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEW
                     GLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6739 a         7311 c         7043 g         6719 t
ORIGIN      
        1 agagataatt cgctttttat caccgagctt gtcccaataa ccaggattaa tcagcgcaat
       61 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
      121 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
      181 ccgtaacgcg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
      241 cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac agcgtcgaaa
      301 gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa tgcctgacat
      361 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
      421 ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa cgatttagcc
      481 aaattcaacc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg
      541 cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca
      601 gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca
      661 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca
      721 ctttcagcaa tacccccacc attgcgatgg ccgccagcgt tctgctgcta gtgccgggct
      781 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
      841 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg atggcactga
      901 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
      961 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc
     1021 gttacgctgg tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga tcttgatgac
     1081 cagcgggttg aatattgagt ggtcaacctt tatggcttct atgctggtcg gtaccattgg
     1141 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     1201 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcgtcggtaa aaatcagcca
     1261 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     1321 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     1381 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     1441 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     1501 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     1561 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     1621 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctag
     1681 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     1741 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt
     1801 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     1861 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     1921 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     1981 gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc agagggtaac
     2041 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     2101 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gggcgatccg
     2161 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     2221 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     2281 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc
     2341 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     2401 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     2461 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct
     2521 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     2581 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     2641 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     2701 tatgcgcctg ggtaacagtt tatgggtgat cttcaactgg gatagctacc gtagccgggt
     2761 aacgggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     2821 agtaatgatg atgaaaacta ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     2881 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     2941 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     3001 cttgcttagc agcggaaacc aagccttaag cgcagataac atgaacaatg ccgcaggcat
     3061 tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa
     3121 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     3181 ggacggtatt cagggtttgc tgaaaacaaa agatggtcag caactcaatc tggataacat
     3241 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     3301 ggggctgaac ttcatttcct gaaccgcgaa atgttacccg gctgacgcgg ctctctcctg
     3361 cccttttccc tgctcctgga cggttttccc cctgttacac gggaagcgac cgcgatcaaa
     3421 aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact agaattgcag
     3481 caatatggcg acgccattac attgtgcttc tcaaaaagct taaacgttgg ctaaacggcc
     3541 cggtttagtg aggatcgccc attgtcagaa ctactctctt tcgccctttt tctcgcctct
     3601 gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttgcagc cacgttaacg
     3661 gtgctggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta ttttactggc
     3721 gatggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg tgctggcgtc
     3781 agcaaataca tcctgccggg tatcggcatt gtgctggggc tgacggcggt gttcggtgcg
     3841 ctgggctgga tcctgcgccg tcgtcgtcat catccgcacc attttggtta cagcctgctg
     3901 gcgctcttac tggcgctggg ttcggtggac gccagcccgg catttcgtca gataacggaa
     3961 ctggtgaaat cccagtcacg cgatggcgac ccggactttg cggcttatta taaagagccg
     4021 tcgaaaacta tccctgaccc gaaactcaac ctggtttata tctacggcga aagtctcgag
     4081 cggacctatt ttgataacga agctttcccg gatctcacgc ctgaactggg cgcgttgaaa
     4141 aatgaaggcc tggatttcag ccacacgcag cagctgccag gaacggatta cacgattgcg
     4201 ggcatggtgg cttctcagtg cggcataccg ctgttcgccc cctttgaagg caacgcctcc
     4261 gcctctgtct ccagcttctt cccgcagaac atctgtctgg gcgatatcct gaaaaactcg
     4321 ggttatcaga actatttcgt gcagggcgca aatctgcgtt ttgccggtaa agatgtgttc
     4381 ctgaaatcgc acggcttcga ccacttatac ggctcagaag agctgaaaag cgtggtagcc
     4441 gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga tgaagcgtgg
     4501 aaaaagtttg aagagctttc ccgctcaggt cagcgattct cactgtttac cctgacagtc
     4561 gatacccatc acccggatgg ttttatctcg cgcacctgta accgcaaaaa atatgatttt
     4621 gacggtaagc cgaatcagtc attcagcgcg gtaagttgca gccaggagaa tatcgcgacg
     4681 tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ctgtcatcgt cgtctcttct
     4741 gaccatttag cgatgaacaa cacggcgtgg aaatacctca ataagcagga tcgcaataac
     4801 ctgttttttg tcattcgtgg cgacaagccg cagcaaaaga cgctggcagt gaagcgtaac
     4861 acgatggata acggcgcgac ggtgctggac attctcggtg gcgataacta tctcggactt
     4921 ggtcgtagca gtttatccgg gcagtcgatg tcggaaatct tcctcaatat caaagagaaa
     4981 acattggcgt ggaagccaga tatcatccgc ctgtggaaat tccctaaaga gatgaaagag
     5041 ttcaccatcg accagcagaa aaacatgatt gccttctcgg gtagccattt ccgtttgccg
     5101 ctgctgttgc gggtttcaga caaacgcgtg gaaccgctgc cggaaagcga atactcagca
     5161 ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca atttcgtctg ggttgacagt
     5221 tgctacaaga tggcacaact ctgggctccg gaactggcac tttccaccga ctggtgtgtc
     5281 tcgcaggggc agcttggcgg tcagcaaatt gttcagcatg ttgacaaaac aacatggaag
     5341 agcaagacgg catttaaaga tacggtcatc gacatggcgc gttacaaagg caatgtcgat
     5401 acgctgaaga ttgttgataa cgatattcgc tacaaagccg acagtttcat ctttaacgtc
     5461 gccggtgcgc cagaagaggt gaaacagttt agcgggattt cccgtccgga gtcgtggggc
     5521 cgctggtcca acgcgcagct gggcgatgaa gtaaaaatcg agtacaagca tccgctgccg
     5581 aagaaatttg acctggtgat taccgccaaa gcatacggca ataacgccag ccgtcctatt
     5641 ccggtacgcg taggcaatga agaacaaact cttgtgctgg gcaatgaagt gaccaccacc
     5701 acactgcatt tcgataaccc aaccgatgcc gacacactgg taattgtgcc gccggaacct
     5761 gtctcaacca acgaagggaa tatcctcgga cactcgccgc gtaagctcgg gatcggcatg
     5821 gtggaaatta aagtggtaga acgtgaaggg taattcctga ctgatgtgta ggtcgcatag
     5881 ggtgcgacct acacgccttc tccggcttac aacacattat taagaaacaa aaaaaacagc
     5941 gcccaacact tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta
     6001 attacaccct taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6061 gtggcgaacg gatagcgatg ggttaacatc atgtcggcag tgattttccc ttccgccatc
     6121 agtcgaccga ctttagcgaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
     6181 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag accgacaaat
     6241 accaccgtgc cgccgtgacg aatcagattc acggtattat tcatcgcatg ttgattacct
     6301 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgcag ctgcgcgtca
     6361 aaatcttcgg ctgacgggtc cagcacgggc aactccagac gcgttgccac atgttcacgg
     6421 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6481 gcgcccagac cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct
     6541 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgcggc cgcctgcggg
     6601 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     6661 ccatcctgat gcacgccaat gactgaaatt ttttcacagc agttggtacg cccgctttta
     6721 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     6781 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaacc
     6841 cgtggataac taaaaaatgg ttgatttcca ccctaggcat gaatatcggt accgcaaatc
     6901 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     6961 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7021 ctatccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7081 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7141 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7201 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7261 tgtacaacat cagccgcact acagtgcgtc atattctcag ccaattacgc gaatgcggcg
     7321 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7381 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7441 cgatgataaa ccagcgtcag ttacgcccta gggaaacttt ttctgaactg caactggctc
     7501 gggccgcggg tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata
     7561 atcttattca aagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     7621 ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     7681 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctagaa cgtcatcggc
     7741 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     7801 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     7861 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     7921 ttgccgtcga tgaacatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     7981 ccacccttgc gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta
     8041 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8101 cacggtttta tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt
     8161 cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     8221 tcaaccttgc gtaatgcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     8281 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     8341 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     8401 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     8461 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     8521 gctttgctct ccgtgttttc actcgcttac ggcattgcgc aacttccttg cggcccacta
     8581 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     8641 caggccatgt ctggcatggt gcacagcttt acgcagttcg tgttggtgcg tatcggtatg
     8701 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     8761 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgct
     8821 gtaagcccac cgattctggc ggcaatgatg ctggtgatgg gctggcgcgg gatgtttatt
     8881 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     8941 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     9001 cgccgagatc cgctcaattt tgccgaatgg cgcagcctgt tccgtaaccg cacaatgtgg
     9061 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     9121 cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct
     9181 atccctttcc tgtttggggc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     9241 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattac cgggatgttc
     9301 tgttctgccg cctttacgct gatagtacca caagcgacaa catccatgat ggcggttctg
     9361 ctgattggca tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc
     9421 cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc
     9481 ttcatctgcg cctctttcgc gccgatcatt actggtttta ttgttgatac aacccactca
     9541 ttccgtctgg cactaatcat ttgcggttgc gtcaccgcag cgggagcact ggcgtacatc
     9601 ttcctggttc gccagccgat caacgacccg cgtaaagatt aaactcccta tgcccgataa
     9661 cattttgctt atcgggcatt tttatggcga ttaaaacgtt tcccagttct ccccgctatc
     9721 tgccactgcc attttacgcg gcgtagctgg cgtcacggtt tttaccacag ccgatgtttc
     9781 acgctgctgt tgctgctgtt gctgaatccg gaacactgcc acagcttcgg tcaggcgact
     9841 ggcctgctct tccagcgcgg cggcggcagc ggcagattct tccaccagcg cggcgttctg
     9901 ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg
     9961 ctcatcagaa gcagaagcta tttcgcccat aatgtccgtc acgcgggtca cagcactgac
    10021 aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc
    10081 caccgagtct tcaatcaggc ttttaatttc acgaaccgcc tgggcgctac gctgggccag
    10141 attacgcact tctcccgcaa ccaccgcaaa accacgtcct tgctcacccg cacgcgctgc
    10201 ttcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gtaatgccgt caattacgct
    10261 gataatatcg gcgattttct gcgaactggt ggagatatcg cgcatagtct gcaccacgtt
    10321 atctaccact ttgccgccgc gttgcgccgt ttcagaagca cttaatgcca gatggctggc
    10381 ctggcgcgca ttctcggcgt tctgtttcac cgttgcggtc agttgctcca tgctggctgc
    10441 cgtctcttcc agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcat tattgccggt
    10501 agcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt
    10561 acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga
    10621 gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgaa tgctgtcaat
    10681 caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac
    10741 cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt
    10801 gttatcgctg acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg
    10861 cttctcgaaa ccgtcctgat atccctgggt cggctgatca aagaactcgt tgattttgcc
    10921 tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt
    10981 gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatccgc
    11041 ccagtttttt tccgcctgtt tcagcgaaat actggcactc tgcatcagct cagcaacggt
    11101 tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt
    11161 gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtgggttgct gctggcgaat
    11221 ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaaa acagaccgcc
    11281 tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt
    11341 gatacgtttt aacatggttt ctctttcctg tggaccgatg caagattttc ggcctggaaa
    11401 gcaaaaactt tatgaaaagg tgtaacccgc catccatgga aaaaccaggc gctggttatc
    11461 agatactaaa aagttattca ttatcgcccg ggatgtcttc ttgtcgggaa ttgcccagag
    11521 caataaattc ctctaacaga tgggttaact gttgcatttt ctcggcagta aactgagctt
    11581 caatctgccg ataggcttct tcaatctgtg tctgagcgcg gttatacagc gcctgcccct
    11641 ctttggtcag cgagatatag agcttccgct gatcattgat cggctttaat cgcaacacta
    11701 aaccgtcgcg ttccatccgc gtgaggatcc cggtcaggct tgggcgcaaa atgcaggcgc
    11761 gatacgccag atcgtgaaaa tccattgatg ggctttccgc cagaatacgc acgatgcgcc
    11821 actgttgctc agtcaaatta tgccgtttaa cgattgggcg aaaataactc atcgccgctt
    11881 ctcgcgcctg gagcaacgca atggttagtg agtcgtgcat tatctttccc ctgaaaaatt
    11941 caaaatcatt aatatagaaa caattaatca tcacgttacc gggactgagc tgcccccgca
    12001 attcttcatt ttatcaaaat tgatgatatt ttattaactt actgatattt aatattttat
    12061 tatctttaaa tgtaaatagt ttgttaatta gatcacattt atatcactta cttttgccaa
    12121 actacgcaat cgggaataaa agtaatcatt aacatattaa tgattaagat gatcgaatcc
    12181 gaggagtggt aatgaaaggc actatcttcg ccgtagcgtt gaaccatcgc agccaacttg
    12241 atgcctggca ggaagcgttc cagcaatctc cctacaaagc cccgcctaaa actgcggtct
    12301 ggtttattaa accgcgcaat acggtgattg gttgcggtga accgattccc tttccacagg
    12361 gtgaaaaagt actgagcggt gcgactgttg cgctgattgt ggggaaaacg gcgacgaaag
    12421 tacgtgaaga agatgcggca gagtacatcg ccggatatgc gctggataac gacgtcagcc
    12481 tgccggaaga gagcttttac cgtccggcaa tcaaagcaaa atgtcgtgat ggattctgcc
    12541 ccattggcga aaccgtggct ctcagcaatg tcgataatct gactatctat accgagatca
    12601 acgggcgtcc tgccgatcac tggaataccg ccgatttaca acgtaacgcc gcacagctgc
    12661 tgagcgccct gagcgaattt gccacgctga atccaggcga tgctattctg ctcggcacgc
    12721 cacaggcgcg cgtggaaata cagccaagcg atcgcgtgcg tgttctcgca gaaggtttcc
    12781 cgccgctgga aaatccggta gtggacgaac gtgaagtgac cacgcgcaag agcttcccaa
    12841 cgctgccaca cccgcacggt acgctgtttg ccctcggcct gaactacgcc gaccacgcca
    12901 gcgaactgga atttaagcca ccggaagagc cgctggtgtt cctgaaagcg ccgaataccc
    12961 tcactggcga caaccagacc tccgtgcgcc cgaacaatat tgaatacatg cactacgaag
    13021 cggagctggt ggtagttatt ggcaagcagg cgcgtaacgt cagcgaagcc gatgccatgg
    13081 attatgtcgc gggctacacc gtgtgtaacg actacgccat tcgcgactat ctggaaaact
    13141 actaccgccc taacctgcgg gtcaaaagcc gcgacggact gacgccgatg ctttcaacca
    13201 tcgtgccgaa agaggcgatc ccggacccgc ataatctgac ccttcgcacc ttcgtcaacg
    13261 gcgagttacg ccagcaaggc accaccgccg atctgatctt cagcgtgccc ttcctgatcg
    13321 cctacttaag cgaatttatg accctgaatc cgggcgacat gatcgccacc ggcacaccaa
    13381 aaggcttatc tgacgtggtg cctggcgatg aagtagtggt ggaagtagaa ggcgtgggcc
    13441 gcctggtgaa ccgaattgtg agtgaggaaa cagcgaaatg aaaaaagtaa atcattggat
    13501 caacggcaaa aatgttgcag gtaacgacta cttccagact accaatccgg caacgggtga
    13561 agtgctggcg gatgtggcct ctggcggtga agcggagatc aatcaggcgg tagcggcagc
    13621 gaaagaggcg ttcccgaaat gggctgatct gccgatgaaa gagcgtgcgc gcctgatgcg
    13681 ccgtctgggc gatctgatcg accagaacgt gcctgagatc gccgcgatgg aaaccgcgga
    13741 cacgggcctg ccgatccatc agaccaaaaa tgtgttgatc ccacgcgctt ctcacaactt
    13801 tgaatttttc gcggaagtct gccagcagat gaacggcaag acctatccgg ttgacgacaa
    13861 gatgctcaac tacacgctgg tgcaaccggt aggcgtttgt gcgctggcgt cgccgtggaa
    13921 cgtgccgttt atgaccgcca cctggaaggt cgcgccgtgt ctggcgctgg gcaataccgc
    13981 ggtgctgaag atgtcggaac tctccccgct gaccgccgac cgcctgggtg agctggcgct
    14041 ggaagctggt attccggcgg gcgtgctaaa cgtggtacag ggctacggcg caaccgcagg
    14101 cgatgcgctg gtccgtcatc atgacgtgcg tgccgtgtcg ttcaccggcg gtaccgccac
    14161 cgggcgcaac atcatgaaaa acgccgggct gaaaaaatac tccatggagc tgggcggtaa
    14221 atcaccggtg ctgatttttg aagatgccga tattgagcgt gcgctggacg ccgccctgtt
    14281 caccatcttc tcaatcaacg gcgaacgctg caccgccggt tcgcgcatct ttattcagca
    14341 gagcatctat ccagaattcg ttaaacgctt tgccgaacgc gccaaccgtc tgcgcgtggg
    14401 cgatccgaac gatccgaata cccaggttag ggcgcttatc agtcagcaac actgggaaaa
    14461 agtctccggc tatatccgtc tcggcattga agaaggcgca accctgttgg cgggcggccc
    14521 ggataaaccg tccgacctgc ctgcgcacct gaaaggcggc aacttcctgc gcccaacggt
    14581 gctggcggac gtagataacc gtatgcgcgt tgcccaggaa gagattttcg ggccggtcgc
    14641 ctgcctgctg ccgtttaaag acgaagccga aggcttacgc ctggcaaacg acgtggagta
    14701 cggcctcgcg tcgtacatct ggacacagga tgtcagcaaa gtgttacgcc tggcgcgtgg
    14761 cattgaagca ggcatggtgt tcgtcaacac ccagaacgtg cgcgacctgc gccagccatt
    14821 tggcggcgta aaagcctccg gcaccgggcg tgaaggcggt gagtacagct tcgaagtgtt
    14881 cgcggaaatg aagaacgtct gcatttccat gggcgaccat ccaattccga aatggggagt
    14941 ctgatatggg taagttagcg ttagccgcca agatcacgca cgtaccgtcg atgtatctct
    15001 ctgaactacc aggaaaaaac cacggttgtc gccagggtgc gatcgacggg cataaagaga
    15061 tcagcaagcg ttgccgggaa atgggcgtcg ataccattat cgttttcgat actcactggc
    15121 tggtcaacag tgcttatcac atcaactgtg cagaccattt tgaaggcgtc tacaccagca
    15181 acgagctgcc gcattttatt cgcgacatga cctacaacta cgagggcaac ccggagttgg
    15241 ggcagctaat tgccgaggaa gccttaaagc tcggcgtgcg ggcaaaagcg cacaatattc
    15301 ccagcctgaa actggaatac ggcacgctgg taccgatgcg ctacatgaat gaagacaagc
    15361 acttcaaagt ggtctccatt tcggctttct gcacggttca cgattttgcc gacagccgca
    15421 agctgggcga agcgattctg aaagcgatcg aacagtacga cggcaccgtg gcggtccttg
    15481 ccagcggttc gttatcgcac cgctttattg acgatcagcg tgcggaagaa gggatgaaca
    15541 gctacacccg cgagttcgac cgccagatgg acgagcgtgt ggtgaagctg tggcgcgaag
    15601 gccagttcaa agagttttgc aatatgctgc cggagtacgc cgactactgc tacggcgaag
    15661 gcaatatgca cgacacggtg atgctgctgg ggatgctcgg ctgggataaa tacgacggca
    15721 aggtggagtt tattaccgag ctgttcccaa gctctggcac cggtcaggtt aacgctgttt
    15781 tcccgctgcc cgcgtaagga cgttttatgc cgcactttat cgtggaatgt actgaaaata
    15841 ttcgtgaaga agcccgctta cctgagctgt ttgccagtgt taacactgca ctggcggcaa
    15901 cggggatttt tccgcttggg ggaatacgta gccgggcgca ctggatcgat acctggcaaa
    15961 tggctgatgg caagcacgat tacgcctttg tgcatatgac gctaaaaatc ggcagcggac
    16021 gcagtctgga aagccgtcag gaagtgggcg aaatgctgtt tgatctgatt aaaacgcact
    16081 tcgcctcact gatggagagc cgttacctag cgctttcatt tgagattgca gagctgcacc
    16141 cgacgttgaa tttcaaacag aacaacgtac acgcgttgtt taaataaatt tgcaccagct
    16201 tccaggccgg atgataaggc gctgccgcat ccggcaatgc cacaggatat cgctatgttc
    16261 gataaacaca cccacaccct gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa
    16321 cagatccgcg cgatctcgct ggattacccg gagatcacca tcgaagacgc ctacgccgtg
    16381 cagcgcgaat gggttcgcct gaaaatcgcc gaaagtcgca cgctgaaagg ccacaaaatc
    16441 ggtctgacct cgaaagcgat gcaggcaagc tcgcagatca gcgaaccgga ttacggtgca
    16501 ctgctggacg acatgttctt ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg
    16561 ccgcgcattg aagtggagct ggcttttgtg ctggcaaaac cgctgcgtgg gccaaactgc
    16621 acgctgttcg acgtttacaa cgccacggac tatgtgatcc cggcgctgga gctgatcgac
    16681 gctcgctgcc acaacatcga tccggaaacc cagcgcccgc gcaaagtatt cgacaccatt
    16741 tccgataacg ccgccaacgc cggagtgatc ctcggaggtc gtccgatcaa accagacgaa
    16801 ctggatctgc gttggatctc cgccctgatg tatcgcaatg gcgtgattga agaaaccggc
    16861 gtcgccgctg gtgtgctgaa tcatccggca aacggcgtgg cctggctggc gaacaaactc
    16921 gcaccgtatg acgtacaact ggaaaccggg caaatcattc tcggtggttc gttcaccctc
    16981 ccggttccgg cgcgtaaggg cgacaccttc cacgtcgatt acggcaacat gggctccatt
    17041 agctgccgct ttgtttaagg agagaacgat ggaaaacagt tttaaagcgg cgctgaaagc
    17101 aggccgtccg cagattggat tatggctggg gctgagcagc agctacagcg cggagttact
    17161 ggccggacca ggattcgact ggttattgat cgacggtgag cacgcgccga ataacgtgca
    17221 aaccgtgctc acccaactac aggcgattgc gccctacccc agccagccag tggtacgccc
    17281 atcgtggaac gatccggtgc aaatcaaaca actgctggac gtaggcacac aaaccttact
    17341 ggtgccgatg gtacaaaacg ccgacgaagc ccgtaaagcg gtacgcgcca cccgttatcc
    17401 ccccgccggt attcgcggtg tgggcagtgc gctggctcgc gcctcgcgct ggaatcgcat
    17461 tcctgattac ctgcaaaaag ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg
    17521 tgaggcaatg aagaacttac cgcagattct ggacgtggaa ggggtcgacg gcgtgtttat
    17581 cggcccggcg gatctgagcg ccgatatggg ttatgccggt aatccgcagc acccggaagt
    17641 acaggccgcc attgagcagg cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat
    17701 cctgatcgcc aatgagcaac tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc
    17761 cgtcggcgtt gacaccaccc tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg
    17821 taatagttct tctatttcga tggcgaagca aaacaacaac tctgtctact aaacgtacga
    17881 tgtggagcgc atcatgacga cctctaccct gcaatataaa gataataaaa ctgttgaaat
    17941 tgaacatcgt gtcattaata aattatttcg tcgcctaatc atttttcttt ttattctgtt
    18001 tgttttttcg tttcttgatc gcatcaatat aggatttgcc ggactgacaa tgggcaagga
    18061 tcttggcctg acgtccacaa tgtttggcct cgccgcaaca ctcttttatg tcacgtacgt
    18121 catgtgcggg atccccagta atatgatgtt agcgaaaatt ggcgctcgtc gatggatcgc
    18181 tgggatcatg gtggtctggg gaattgcttc cacctgcaca atgtttgcaa caagccccga
    18241 aacactctat ctactgcgta tgctggttgg tattgctgag gcgggttttt tacctggtat
    18301 tttggtttat ctgacctggt ggttcccggc atatcatcgg gccagagcca atgccttatt
    18361 tatgatagcg atgccagtca ctatgatgct gggattgatc ctttcaggct acattctggc
    18421 cttggatgga ctgtggaatc taaaaggctg gcaatggctc tttttgctgg aaggtctgcc
    18481 atcggtcatt cttggtgtcg tcacctggtt ttatctcaac gacacaccag accaggcgac
    18541 ctggcttgat gaaaacgaaa aacaggcttt aaaaacgatg attctccgtg aacaagagag
    18601 tgcatttgcc catgcatctg ttccacgctc aaccttacgt gaagtcctga ccccagccat
    18661 catgctatat acacttgctt atttttgccc gactaatact ctgagtgcga ttaatatctg
    18721 gacgccgcaa atcctacaga gtttcaatac tggcagtagc aacatcatga ttggtttgct
    18781 ggcggccatc ccccaattct gcacaatttt tggcatgatc tggtggagtc gtcgttccga
    18841 ccgtcttaaa gaacgcaaaa agcatacgat tcttccctac ctgtttgccg ctgccggatg
    18901 gttactcgct tctgcgacag atcacagcct cattcaactg agcagcatca ttatggcctc
    18961 aatgggatca tttaccgcga tgactatctt ttggacaaca ccggatcaag ttataagctt
    19021 gcaatcgcgt gccgttgcga tggctgtcat caatgctgtc ggcaatgtag gttcagcagt
    19081 cagtccatta cttatcggta ttttgaaaga tgcaaccggc agttttagct caggtttgtg
    19141 gtttgtagca ggattgctgg tactgggcgc gttagtcatg acccgaatcc ctatggggag
    19201 tcgggaacac tctctttcat ctgaacttat cgcaccaaaa ccgcaataag gagtcatgat
    19261 gtccagtgaa gctatctttg aacatggtta tgaaaatatt gatttggtcc gggaatatga
    19321 tgcccgctac gctggtgaag atgtgcatta tgaaacattt gcccggttag cgaatttctt
    19381 tggacgcgat atgcgccctc actggcacga tagatatttt caactccatt atctggtcac
    19441 tggacgcatt actttacaac tggatgagca cttctattct gtgcgcgcac cgctttttgt
    19501 actaacacca ccatcggtgc cacatacttt ctttctttac tgatcctgat agcgatgggc
    19561 atgttctgac cgtacgtcag gagttaattt ggcccctggt agaaaagctc tggcctggta
    19621 gtggagatgc cgcaatgcta caaggtatct gtctgtcact ggagggaatg gaacatacac
    19681 tggcggcatt aaatcattat tggccgataa tatccctgga gtttcaacaa aataagcaag
    19741 gacgggaaat tctattgcaa tcactggcgc aaagtatttt tacaatccta ctgcgtgaag
    19801 caccaccaaa tgatatttca acatgcagcg tgcgcggcga gatgtgttta ttccagaagt
    19861 ttaatcggct gattgacgaa aattatcgcc aacatttaat ggtccctgaa tatgcaacaa
    19921 tgctcggtat gagtgaatca cggcttaccg agctgtgccg acgttttgcc aatcaatcgc
    19981 cgaagagatt gattttcgaa cgagtgagcc gggaagccag acggcttctg ctctatagcg
    20041 agcaaagcat caaccagata gcccttgatc ttggctacaa agatcccgcc tatttcgccc
    20101 gttttttcaa ccgaatgatg ggttgctcac caacacaatt ccgtggtcgc tgaccattta
    20161 tcaacctgag agactcgaca tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat
    20221 tgcgccagtg gcattcacct acgcctccga accgcaataa atgaaaattg tactacttgg
    20281 aacgaaaggt ggcccttctt tattaaaagc tgaccgttta ccgatggcaa cggcacttat
    20341 tattggcaat aaaacctggt taattgatgc tggttacggg gccagcttac aactggtaaa
    20401 aaataatatt ccactgcgcc agattgataa tattttactc acccatttac acagtgatca
    20461 tattctggac tatccatcag tcttaatgaa tgcatgggct agcggtctgc aggacaaaac
    20521 tattcatgta ttcggtccgc caggcacccg agccatgacc gctgccagtt ggcaggtctt
    20581 cgatcgggac atcacactgc gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt
    20641 tacagctcaa gatgttggtg aaggcgtagt ttataaagat gataccgtga ccgtgactgc
    20701 attaaaggtc cctcattctc ctttccctga cggtgaggct tttgcttatc gcttcgacac
    20761 acaaggcaag cgaattgtct tctcaggaga tacctcgtac tttcccccac tggcaacttt
    20821 ctcagaaggc gctgatatac ttgtgcatga ggccgtacat gagccctccg tcgctaagct
    20881 ggcagacagt attggcaatg gtaaaacact ggcaaaagcc attgcttctc accatacgac
    20941 tattgaggat gtaggcaaaa ttgccaatga agcaaaagtg aaaaaactca tccttagcca
    21001 cctggtacca atcaccgttc cggatgaaac atggaagcaa gaagcacaga aaactattca
    21061 ggtcctatcg tcgttggtca tgaaaatatg acgattacca ttccattcgc cccataaaaa
    21121 attccgtccc cgttagccct tgttagcggg gaatattatg atttgcctta ctgtaagttg
    21181 atcacgatcg cagtttacta tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc
    21241 tgaaacgtct cttcccgaca accgcgttca acgcttcaat taaataacaa aaacaacaca
    21301 caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac cagaagattt
    21361 ccgcgccagt acccaacgtc ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg
    21421 tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc accactcatc cggcatttcg
    21481 taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg agatgcagga
    21541 ctctctgtgt tggaacaccg acaccggcag cggcggctat acccataaat tcttccgcgt
    21601 ggcgaaaagt gccgacgacc tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct
    21661 gagctatggc tggatgggcc gtaccccaga ctacaaagcc gctttcggtt gcgcactggg
    21721 cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt atacccgtat
    21781 tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg tcatttgccg
    21841 accgataaag tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc
    21901 gtcagcggtg cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc
    21961 ttcggctcgg cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca
    22021 atggatgccg atggcgtgaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct
    22081 accggctcgc catacgacta cccgctctcc agccgcttcg atgagaacga tgcgattctg
    22141 gtgatggata acgtactgat cccatgggaa aacgtgctga tctaccgcga ttttgatcgc
    22201 tgccgtcgct ggacgatgga aggcggtttc gcccgtatgt atccgctgca agcctgtgtg
    22261 cgcctggcag tgaaactcga cttcattacg gcactgctga aaaaatcact cgaatgtacc
    22321 ggcaccctgg agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac
    22381 accttctggg cattgagtga ctcgatgtgt tcagaagcga cgccgtgggt caacggggct
    22441 tatttaccgg atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg
    22501 aagatcaaaa acattataga acgcaacgtt accagtggcc tgatctacct cccttccagt
    22561 gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg
    22621 aacggtatgg atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc
    22681 agcgaatttg gtggtcgtca cgagctgtat gaaatcaact actccggtag ccaggatgag
    22741 attcgcctgc aatgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg
    22801 atggttgatc gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac
    22861 aacaacgacg atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga
    22921 tgcaattaga tgaacaacgc ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg
    22981 taaatattat caccaccgag ggcgacgccg gacaatgcgg gattacggca acggctgtct
    23041 gctcggtcac ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga
    23101 acccggtttt tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac
    23161 tgatggcgcg ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct
    23221 catgctggca aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg
    23281 aaggagagat ccgcgatgtg caggcgattg gcacacatct ggtgtatctg gtggagatta
    23341 aaaacatcat cctcagtgca gaaggtcacg gacttattta ctttaaacgc cgtttccatc
    23401 cggtgatgct ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa
    23461 ggggcatttt tattgtgatc tcaatcacat atctacccga tgaataattc ttgcccaacc
    23521 gctcaccgcc attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac
    23581 tttacgcgca cgtatctctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa
    23641 acgcaacgca aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg
    23701 gtttgcagga aggcagcaag tgagtgaatc cccgggagct tacaaaagta agtgtcagag
    23761 gtgaacgaac gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt
    23821 tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg
    23881 tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat
    23941 cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc
    24001 ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg
    24061 tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc
    24121 cggtgctggt ccgctggtgg gaccggttct cgccgcgcag atgggctacc tgcctggcac
    24181 gctgtggctg ctggcggggg tagtgctggc cggtgcggta caggacttta tggtgctgtt
    24241 tatctcctct cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatggggcc
    24301 agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt
    24361 tctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct tcaccgtttg
    24421 ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg
    24481 tgtgggtgaa gtctctgtta ttggtatcgt gctgctggtt gcctctatct acttcggtgg
    24541 cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac
    24601 cttcgcgctg attggctatg cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc
    24661 accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg
    24721 tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac
    24781 tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc
    24841 ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc tggctaacga
    24901 aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat
    24961 ggcgctggtt gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga acaccccgcc
    25021 tgctggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat
    25081 catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca gctcctgggg
    25141 cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt
    25201 cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcacg tgttccacaa
    25261 agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct
    25321 gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaagacct
    25381 gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg
    25441 tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct
    25501 gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt
    25561 agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt
    25621 aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact
    25681 gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa
    25741 gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt
    25801 gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat
    25861 cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga
    25921 aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg
    25981 tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag
    26041 gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggtatt ccagactacg
    26101 acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag
    26161 aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct
    26221 gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc
    26281 gcgggcaaaa ctaccctgct gcgccatatt cttaacgaac aacacggcta caagattgcc
    26341 gtgattgaaa acgaattcgg cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc
    26401 acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggag
    26461 gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg
    26521 gtgattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt tttctcccat
    26581 gagattttat gccagctcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat
    26641 gccgatgagc agatgaacca gttcaccatc gcccagtccc aggtgggcta cgccgaccgt
    26701 attctgctga ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg
    26761 cgcatcaacg cccgcgcacc ggtctacacc gtcacccacg gcgacatcga cctgggcctg
    26821 ctgttcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc
    26881 cactttatcg cggataaaca aaacgatatt tcgtcaattg tggtggaact ggattacccg
    26941 gtagatatca gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa
    27001 ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct gctgttccag
    27061 ggcgtccagc gcctctacag cgccgactgg gacaggccgt ggggcgatga aaaaccgcat
    27121 agcacaatgg tgtttatcgg gattcagttg ccggaggaag agattcgggc ggcgtttgcg
    27181 ggattgagga agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt
    27241 acgagtggaa ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt
    27301 ttttcatgtc tcattttcag ttcagccaat ccccattctg aagaattaca aacatctttt
    27361 gttaaataaa aagagctttt actattaagt ttctcataaa tagatttctt tttgataaaa
    27421 tcatagtgtt gtgtttcaga atttttggaa ccacttatga gtgttaaatt acctatttta
    27481 tgcagccagt agacatgctc ttgaggttgg aatctattaa tccagtattc attaactaat
    27541 gcttgaggaa gaatatgctc aattgtgatt ctccccgtat aggttttaat cacgctctca
    27601 tcttgttgct ccatatctaa gcgaagcaaa attgctttta ttaagttcac ttgattcgga
    27661 cgaggctcat ataaatcttc atccagagca gctataaaac ctgaattatc tgcatgttgg
    27721 tttatctgat ttatcacaga atcaaaaggc atatcattat ttattgctac cagagcagaa
    27781 taacaaacca tttctctttg gcttttaatt tg
//
LOCUS       DAJTYL010000048        27773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYL010000048 DAJTYL010000000
VERSION     DAJTYL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.048"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..691)
                     /gene="dapA"
                     /locus_tag="OO572_001979"
     CDS             complement(<1..691)
                     /gene="dapA"
                     /locus_tag="OO572_001979"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6660993.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEAR"
     gene            837..1409
                     /locus_tag="OO572_001980"
     CDS             837..1409
                     /locus_tag="OO572_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6660994.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1409..1879
                     /gene="bcp"
                     /locus_tag="OO572_001981"
     CDS             1409..1879
                     /gene="bcp"
                     /locus_tag="OO572_001981"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6660995.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            2132..2749
                     /gene="hyfA"
                     /locus_tag="OO572_001982"
     CDS             2132..2749
                     /gene="hyfA"
                     /locus_tag="OO572_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6660996.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            2749..4766
                     /gene="hyfB"
                     /locus_tag="OO572_001983"
                     /pseudo
     CDS             2749..4766
                     /gene="hyfB"
                     /locus_tag="OO572_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            4777..5724
                     /gene="hyfC"
                     /locus_tag="OO572_001984"
     CDS             4777..5724
                     /gene="hyfC"
                     /locus_tag="OO572_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6660997.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            5741..7180
                     /gene="hyfD"
                     /locus_tag="OO572_001985"
     CDS             5741..7180
                     /gene="hyfD"
                     /locus_tag="OO572_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6660998.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            7192..7842
                     /gene="hyfE"
                     /locus_tag="OO572_001986"
     CDS             7192..7842
                     /gene="hyfE"
                     /locus_tag="OO572_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6660999.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            7847..9433
                     /gene="hyfF"
                     /locus_tag="OO572_001987"
     CDS             7847..9433
                     /gene="hyfF"
                     /locus_tag="OO572_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6661000.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            9423..11137
                     /gene="hyfG"
                     /locus_tag="OO572_001988"
                     /pseudo
     CDS             9423..11137
                     /gene="hyfG"
                     /locus_tag="OO572_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            11147..11692
                     /gene="hyfH"
                     /locus_tag="OO572_001989"
     CDS             11147..11692
                     /gene="hyfH"
                     /locus_tag="OO572_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6661001.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            11689..12447
                     /gene="hyfI"
                     /locus_tag="OO572_001990"
     CDS             11689..12447
                     /gene="hyfI"
                     /locus_tag="OO572_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6661002.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            12440..12853
                     /gene="hyfJ"
                     /locus_tag="OO572_001991"
     CDS             12440..12853
                     /gene="hyfJ"
                     /locus_tag="OO572_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6661003.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            12883..14712
                     /gene="hyfR"
                     /locus_tag="OO572_001992"
     CDS             12883..14712
                     /gene="hyfR"
                     /locus_tag="OO572_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6661004.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            14713..14895
                     /locus_tag="OO572_001993"
     CDS             14713..14895
                     /locus_tag="OO572_001993"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6661005.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            14917..15789
                     /locus_tag="OO572_001994"
                     /pseudo
     CDS             14917..15789
                     /locus_tag="OO572_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(15827..16888)
                     /locus_tag="OO572_001995"
     CDS             complement(15827..16888)
                     /locus_tag="OO572_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6661006.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            17101..18564
                     /gene="bepA"
                     /locus_tag="OO572_001996"
     CDS             17101..18564
                     /gene="bepA"
                     /locus_tag="OO572_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6661007.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            18585..18944
                     /gene="arsC"
                     /locus_tag="OO572_001997"
     CDS             18585..18944
                     /gene="arsC"
                     /locus_tag="OO572_001997"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6661008.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(19077..19823)
                     /gene="hda"
                     /locus_tag="OO572_001998"
     CDS             complement(19077..19823)
                     /gene="hda"
                     /locus_tag="OO572_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6661009.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(19873..21162)
                     /gene="uraA"
                     /locus_tag="OO572_001999"
     CDS             complement(19873..21162)
                     /gene="uraA"
                     /locus_tag="OO572_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6661010.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(21248..21874)
                     /gene="upp"
                     /locus_tag="OO572_002000"
     CDS             complement(21248..21874)
                     /gene="upp"
                     /locus_tag="OO572_002000"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6661011.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            22198..23235
                     /gene="purM"
                     /locus_tag="OO572_002001"
     CDS             22198..23235
                     /gene="purM"
                     /locus_tag="OO572_002001"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6661012.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            23235..23873
                     /gene="purN"
                     /locus_tag="OO572_002002"
     CDS             23235..23873
                     /gene="purN"
                     /locus_tag="OO572_002002"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6661013.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            24045..26111
                     /gene="ppk1"
                     /locus_tag="OO572_002003"
     CDS             24045..26111
                     /gene="ppk1"
                     /locus_tag="OO572_002003"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6661014.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            26116..27657
                     /gene="ppx"
                     /locus_tag="OO572_002004"
     CDS             26116..27657
                     /gene="ppx"
                     /locus_tag="OO572_002004"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6661015.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         6359 a         7260 c         7321 g         6833 t
ORIGIN      
        1 cgcgtgcctc ggcaaaatgc ccttctgctg ccagtttgca catctgggcc atatcacgcg
       61 ctgcgacgtt agccgtaacg gaaataactc catgaccgcc caattgcatg aagtccagcg
      121 cgctcgcatc atcgccgctc agcagaacaa aatcatctga aaccagctct ttgatctggt
      181 ttacacgcgt taagttccct gttgcctctt tgattccgat aatatttttt actttcgcca
      241 gacggcccac cgtttccggg agcagatcgc agccagtacg ggacggcaca ttatacagaa
      301 tttgcggcag gtcagtatgc tcagcgatgg ctttgaaatg ctgatacaaa ccttcttgcg
      361 acggacgatt gtagtaaggg gttaccgtca ggcagccgac gataccactg tcattgaagc
      421 gctgcgtcag gctaatggct tccgcagtag cgttagcgcc ggtcccggca attaccggaa
      481 tgcgcccatc agccagatcc agcgtcatca tcaccacatc agcatgttcg tcatgattta
      541 aggtagcgga ctcgccagtg gtgccaacag aaacgatcgc cgaagtaccg ctggcgacat
      601 gataatcaat cagttttttc aagctagccc gacagacatt acctttttca tccatcggag
      661 taacaatcgc gacaatactt cccgtgaaca tgggccatcc tctgtgcaaa caagtgtctc
      721 aatggtacgt ttggtatggc attaaaagca agcagacaga accgttctga ttgttgtatg
      781 catgattttt ttatgctttc cttaagaaca actcacccct taaaggaata accagtttga
      841 cactgtcatc gcaacattat ctggtgatca ctgcgttggg tgccgatcgc cctggaattg
      901 tgaacaccat cacccgtcat gtcagtagtt gcggctgtaa tattgaagac agtcgcctgg
      961 cgatgctggg agaagagttc acgtttatca tgctgctttc cggttcatgg aatgccatca
     1021 ctctgattga atcaacgtta ccgttgaaag gtgccgaact ggatctttta atcgtgatga
     1081 agcgcacgac ggcgcgtccg cgtccgccaa tgccagcatc tgtctgggtt caggtcgatg
     1141 ttgcagactc cccgcattta attgaacgct tcacagcact tttcgacgcg catcatatga
     1201 acattgcgga gctggtgtcg cgcacacaac ctgctgaaaa tgaacgggct gcgcagttgc
     1261 atattcagat aaccgcccac agccccgcat ctgcggacgc agcaaatatt gagcaagcgt
     1321 tcaaagccct atgtacagaa ctcaatgcac aaggcagtat taacgtcgtc aattattccc
     1381 aacatgatga acaggatgga gttaagtaat gaatccactg aaagccggtg atatcgcacc
     1441 gaaatttagc ttgccggatc aagacggaga acaagttaat ttgaccgact tccagggaca
     1501 gcgtgttctg gtttatttct acccgaaagc catgaccccc ggctgtaccg tacaggcctg
     1561 cggcttacgc gataacatgg atgagttgaa aaaagcgggc gttgatgtgc tgggtatcag
     1621 caccgataaa cccgaaaaac tctcccgttt tgcggaaaaa gagctgctta actttacgct
     1681 cctgtctgat gaggaccacc aggtgtgcga acaattcggc gtctggggtg aaaagtcctt
     1741 catgggcaaa acctacgatg gtattcatcg catcagcttc ctgattgacg ctgatggcaa
     1801 aatcgaacat gtctttgacg atttcaaaac cagcaatcac cacgacgttg tgctgaactg
     1861 gctgaaagaa cacgcctgat tactttgctc cattccgtgc tggctgcgct tgcggccagc
     1921 atacctcact tctcgtgatc aagatcacat tctcgctttc ccctgcgaca cgggtgtcga
     1981 atccattttt tgctgaacgt taatgaccat catttttgta ccgttcagaa tccagttaat
     2041 acataactta ttgaatagat tgagttaatc agaatggcat cctttataca atatgaaatg
     2101 caatgtttca tatcattttc aaggagccga catgaaccgc tttgtggtgg ccgaaccact
     2161 gtggtgtaca ggatgtaata cctgtctcgc tgcctgttcg gacgtgcata aaacgcaagg
     2221 tttacagcaa cacccgcgcc tggccctggc gaagacgtca acaatcactg cccctgtcgt
     2281 gtgtcatcac tgtgaggaag ccccttgcct gcaggtctgc ccggtcaatg ccatctctca
     2341 gagggatgat gcgatccaac tcaacgaaag cctctgtatt ggctgcaagc tttgcgccgt
     2401 ggtctgccca tttggcgcaa tcagcgcttc aggaagccgt ccggtgaatg cccatgcgca
     2461 atatgttttt caggctgaag gctcactcaa agacggcgaa gaaaacgtgc cgccccaaca
     2521 tgcgttgctg cgctgggaac cgggtgtcca gaccgtcgcg gtgaaatgcg acctgtgtga
     2581 tttcttgcca gaaggtccgg cctgcgttcg cgcttgcccg aatcaggcgt tacggctgat
     2641 caccgacgat agcctgcaac gtcagatgaa agaaaaacag cgccttgccg caagctggtt
     2701 tgccaatggc ggggagtctc ccctttccct cactcaggag caacactaat ggatgccctg
     2761 caattattaa cctggtcgct gatcctctat ctgtttgcca gtctggcttc gctgttttta
     2821 ctcggtctgg acagactggc tattaagctt tccggcatca catcgctggt gggcggcgtg
     2881 attggcatca tcagcggaat tacgcaatta catgctggtg taactttagt cgcccatttt
     2941 gccacccctt ttgactttgc cgatttaacc ctgcgaatgg atagcctctc ggcatttatg
     3001 gtgctagtta tctccttgct ggtggtggtt tgttcgctct attcattgac ttatatgcgc
     3061 gaatacgagg gcaaaggcgc ggcggcgatg ggcttcttta tgaatctttt catcgcatcg
     3121 atggttgccc tgctggtgat ggacaacgct ttttggttca tcgtgctgtt tgaaatgatg
     3181 tcgctgtctt cctggtttct ggtcattgcc aggcaggata aaacgtcgat caacgctggc
     3241 atgctctact tttttatcgc ccacgccgga tcggtgctga ttatgatcgc cttcttgctg
     3301 atggggcgcg aaagcggcag cctcgatttt gccagtttcg cacgctttca ctttctccgg
     3361 ggctggcgtc ggcggtgttt ctgctggcct ttttcggttt tggcgcgaaa gccgggatga
     3421 tgccgttgca cagctggttg ccgcgcgctc accctgccgc accatcgcac gcttcggcgt
     3481 tgatgtctgg cgtaatggtc aaaatcggta ttttcggcat cctgaaagta gcgatggatc
     3541 tgctggcgca aacgggtttg cctctgtggt ggggcattct ggtgatggcg atcggcgcaa
     3601 tctccgcgct cctgggcgtg ctatatgcgc tggcggaaca ggatatcaaa cggctgctgg
     3661 cctggagtac cgtcgaaaac gtcggcatta ttttgctggc agtcggtgtg gcgatggacg
     3721 gtctgtcact gcacgacccg ctgctcaccg tggttggact gctcggcgca ctgtttcatc
     3781 tgctcaacca tgcgctgttc aaagggctgc tatttctcgg cgcggacgcg attatttcgc
     3841 gtttgcatac ccacgacatg gaaaaaatgg gggcactggc gaaacggatg ccgtggacag
     3901 ccgcagcatg cctgattggt tgcctcgcga tatcagccat tcctccgctg aatggtttta
     3961 tcagcgaatg gtacacctgg cagtcgctgt tctcactaag tcgtgtggaa gccgtagcgc
     4021 tacaacttgc gggtcctatt gctatggtaa tgctggcagt cactggtggg ctggcagtaa
     4081 tgtgcttcgt caaaatgtac ggtattactt tctgtggtgc gccgcgcagt acacacgctg
     4141 aagaggcaca ggaagtgcca aatacgatga tcgtcgccat gctactgctc gcggcactct
     4201 gcgtattaat tgcgcttagt gccagttggc tggcaccgaa gataatgcat attgcccatg
     4261 cgtttaccaa tacccctccc gtcactgtcg ccagcggaat agcacttgta cccggcacgt
     4321 ttcatacacg ggtcactcct tcattactgt tgctgttact actggcgatg cctttgctgc
     4381 caggccttta ctggctgtgg tgtcgttcgc gccgcgcagc gtttcgtcgc acaggagatg
     4441 cctgggcatg cggctacggc tgggaaaacg cgatggctcc gtcaggcaat ggcgtgatgc
     4501 agccgctgcg tgtggtcttc tctgcgttat ttcgtctacg acaacagctc gaccctacgc
     4561 tgaggctaaa taaaggtctt gcgcacgtca ccgccagggc tcagagcaca gaacccttct
     4621 gggatgagcg ggtgatccgc cccatcgtga gcgccaccca acggctggtc aaagaaatac
     4681 agcatctgca aagcggcgac tttcgtctct attgcctgta tgtggtcgcc gcactggttg
     4741 tgctgctaat cgctattgcc gtctaaggaa atcaccatga gacaaactct ttgcgacgga
     4801 tatctggtca tttttgcgtt agcacaggcc gtgattctgc tgatgctaac cccacttttt
     4861 acgggtattt cccggcagat acgcgcgcgt atgcactccc gccgcgggcc ggggatctgg
     4921 caggattatc gcgatatcca caaactgttt aaacgccagg aagttgcgcc gacatcttca
     4981 ggtctgatgt tccgcctgat gccgtgggta ttaatcagca gcatgctggt gctggcgatg
     5041 gccttaccac tatttattac cgtttcccct tttgcgggcg gcggcgatct gatcaccctt
     5101 atctatcttc ttgccctgtt tcgttttttc tttgctcttt ccgggctgga taccggaagt
     5161 ccgtttgcgg gagtcggtgc cagtcgcgag ttgacgctcg gcattctggt cgaaccaatg
     5221 cttattctct cactgctggt attggcgctg atagcaggtt ccacgcatat cgagatgatc
     5281 agcaatacgc tggctatggg ctggaactcg ccgctaacca ccgtactggc gttactggcc
     5341 tgtggttttg cctgcttcat tgagatggga aaaattccct ttgatgttgc tgaagcagaa
     5401 caggaattac aggaaggccc gctgaccgaa tattccggtg ccgggctggc gctagcgaaa
     5461 tgggggctgg ggctgaaaca ggtcgtgatg gcagcactgt ttgtggccct gtttctgccc
     5521 tttgggcgcg cgcaagaact ttctctcgcc tgcctgctga cttcacttgt cgttacgctg
     5581 ctcaaggttt tgctgatttt tgtactggcc tcaatcgcag aaaacacgct ggcacgcggg
     5641 cgttttttac tcattcacca tgtgacctgg cttggcttca gccttgctgc gcttgcatgg
     5701 gtcttttggt taaccggtct gtaaggagca ctgacggaat atggaaaatc ttgctctgac
     5761 gacgttaatg ctgcctttta tcggcgcact ggtcgtttcg ttttcgccac aacgtcgggc
     5821 cgccgaatgg ggggttttgt tcgccgcgct gaccacgctg tgcatgttgt cgctgatctc
     5881 cgcgttttat caggccgata aagttgccgt cacgttgacg ttggtcaacg tgggggatgt
     5941 ggcattattt ggcctggtca ttgatcgcgt gagtacgctg attctgtttg tggtggtgtt
     6001 tctcggtttg ctggtcacga tctactccac gggttatctg acggataaaa atcgcgaaca
     6061 cccgcataac ggcacgaatc gttattacgc atttttgctg gtgtttatcg gcgcgatggc
     6121 gggactggta ctctcctcga cgctgctcgg tcagttgttg ttttttgaaa ttacgggcgg
     6181 ctgctcctgg gcgttgatca gttattacca gagcgataaa gcgcagcgtt cagcactaaa
     6241 agcgttgctt atcacccata tcggttcgtt ggggttgtat cttgccgccg ccacgctgtt
     6301 tttgcagacc ggaacgtttg tgcttagcgc gatgagcgag ttacatggcg acgcacgtta
     6361 tctggtttat ggcggcatcc tgtttgccgc gtgggggaaa tcggcccagc taccgatgca
     6421 agcgtggcta ccggacgcaa tggaagcgcc aacaccgatc agcgcctatc tccacgccgc
     6481 atcgatggtg aaagtgggcg tttacatttt tgcccgcgcc attatcgacg gcggcaatat
     6541 cccgcatgtg attggcggcg ttggcatggt catgacactg gtcaccattc tttatggctt
     6601 tctgatgtat ttgccacagc aggatatgaa gcggttgctg gcctggtcga ccatcactca
     6661 acttggctgg atgttcttcg gcttgtcgct ctccatcttc ggctcgcggc tggcgctgga
     6721 gggtagcatc gcctacatcg tcaaccacgc gttcgctaaa agcctgtttt tccttgtagc
     6781 aggtgcgctg agttacagct gcggcacgcg cttgttgccg cgtctgcgtg gcgtattgca
     6841 caccctgccg ttgccaggcg tgggtttctg cgtggcagcg ctggcaatta ccggtgtgcc
     6901 gccgttcaac ggcttcttca gtaaattccc gctgtttgcc gccggttttg cgttgtcagt
     6961 ggagtactgg atcctgctgc ccgccatgat tctgctgatg attgaatcgg tcgccagttt
     7021 cgcctggttt attcgctggt ttggtcgcgt cgtgccgggc aaaccgagcg aggccgtcgc
     7081 cgatgccgca ccgctgccag ggtcaatgcg cctggtgttg attgtactga ttgtgatgtc
     7141 gctgatttcc agcgtaatcg ccgcgacctg gttgcagtaa ggagatgatg aatgaccggt
     7201 tctatgatcg taaataatct ggcgggactg atgatgctga catcgctgtt tgtgattagc
     7261 gtcaaaagct atcgcctgtc atgcggattt tacgcctgcc agtcgctggt gctggtgtct
     7321 attttcgcca ctctctcgtg cctgttcgcc acagagcaac tgctgatctg gtccgccagc
     7381 gcctttatca ccaaagtgct gctggtaccg ttaatcatga cttacgctgc acgaaatatt
     7441 ccccagaaca tcccggaaaa agcgttattc ggtccggcaa tgatggcact gctcgcggcg
     7501 ttaattgtcc tgctttgcgc atttgtcgtt cagcccgtga agctaccgat ggctaccggg
     7561 ctgaaaccgg cgctggcggt agcgttaggt cattttctgc ttggcctgct gtgcattgtc
     7621 agccaacgca atatcctgcg gcaaattttt ggttactgcc tgatggaaaa cggctcccat
     7681 ctggtgctgg cgcttcttgc ctggcgagca ccggaactgg tggaaatagg tatcgctacc
     7741 gacgccatct tcgccgtcat tgtgatggtg ttactggcaa gaaaaatatg gcgtacccac
     7801 ggcacgctgg acgtgaacaa cttgaccgcg ctgaagggat aatgagatga gttattctgt
     7861 gatgttcgct ttactcctgc tcacgccgct gcttttttcg ctgctctgtt ttgcctgccg
     7921 gaaacggggg ctctctgcga cttgtacggt gactgtatta catagcttag ggatcacact
     7981 gctgctgatt ctagcactct gggtggtcca aactgccgct gatgcaggag aaatattcgc
     8041 tgcgggactg tggcttcata ttgatggtct gggcggtttg ttcctcgcca ttcttggtgt
     8101 gattggcttt ctcaccggtg tttactcgat tggctacatg cgtcatgaag tggcacacgg
     8161 cgagctttca cccgttacac tgtgcgatta ctacggtttc ttccatctgt ttttgttcac
     8221 catgctgctg gttgttacca gcaataacct gattgtgatg tgggcggcga tcgaagccac
     8281 caccttaagc tcggcgtttc tggtaggcat ttacggtcag cgttcatcgc tggaatcagc
     8341 atggaagtac atcattattt gtactgttgg tgtcgctttt ggtctgttcg gtaccgtgct
     8401 ggtatacgcc aacgccgcca gcgttatgcc gcaggcagaa atggcgatat tctggagcga
     8461 ggttcttaag caatcgtcct tgcttgaccc aacattaatg ctgttggcct ttgtgtttgt
     8521 gctaattggc tttggtacca aaaccgggct atttcccatg cacgcctggc tgccggatgc
     8581 tcacagtgaa gcgccgagtc cggtcagcgc cctgctctcc gccgtattgc tgaactgcgc
     8641 gctgttggtg ctgattcgct attacatcat tatttgccag gccatcggca gcgatttccc
     8701 caaccggttg ttgctcatct tcggcatgtt gtcggttgcc gtggcggcat ttttcattct
     8761 ggtacagcgg gacattaagc gtctgctggc gtactccagc gtggagaaca tggggctggt
     8821 cgcggtggcg ctaggcattg gcgggccgct gggaattttt gccgcgctgc tgcacacctt
     8881 aaaccacagt ctggcaaaaa cgctgctgtt ctgcggttcc ggcaatgtac tgctcaagta
     8941 cggcacgcgc gatctcaacg tcgtctgtgg gatgctcaaa atcatgccat ttaccgccgt
     9001 gctgtttggc ggcggtgcgc tggcgctggc gctggcaggg atgccgccct tcaacatttt
     9061 tcttagcgaa tttatgaccg ttaccgcagg actggcacgt aatcacctgc tgattatcgt
     9121 cctgctgtta ttgctgttaa cgctggtgct ggcgggcctg gtacggatgg ctgcgcgggt
     9181 gttaatggcg aaaccgccgc aggccgttaa ccggggtgat ctcggctggt tgaccacctc
     9241 gccaatggtg attctgctgg tcatgatgct ggcgatggga acgcatattc cacaacctgt
     9301 catcaggatc ctggcgggcg cttccactat agtcctctca gggacgcacg acctgcctgc
     9361 acaacgtagc acctggcatg attttttgcc ttcaggcacc gcatctgttt cggagaaaca
     9421 cagtgaacgt taattcatcg tcaaatcgtg gcgaagcgat tctcgccgcc ctgaaaacgc
     9481 agttccccgg cgcggtgctg gatgaagagc gacaaacgcc tgaacaggtc accattacgg
     9541 tgaaaatcaa tctgctgcct gacgttgtac attatcttta ttatcaacat gatggctggc
     9601 ttccagtcct gtttggcaac gacgagcgga cacttaacgg tcattacgcg gtttattatg
     9661 ccctttcaat ggaaggggcc gaaaaatgct ggatcgtggt gaaggcactg gtcgatgccg
     9721 acagtcggga gtttccgtca gtcacaccgc gcgtccctgc cgcggtctgg ggcgagcgag
     9781 aaattcgcga tatgtacggg ctgattccgg ttggcctgcc ggatcagcgt cgcctggtgt
     9841 tgcccgatga ctggccggaa gatatgcatc cgctgcgcaa agatgcgatg gattatcgac
     9901 tgcgccctga accgacgaca gattccgaaa cgtatgcgtt tatcaatgag ggcaacagcg
     9961 atgcgcgggt gatccctgtc ggcccgctgc atatcacctc ctatgaaccg ggtcacttcc
    10021 gcttgtttgt ggatggcgag caaattgtcg atgctgatta ccgcctgttt tatgtccatc
    10081 gcggcatgga gaaactggca gaaacgcgga tgggctacaa cgaagtgacc ttcttatcgg
    10141 accgcgtgtg tgggatttgc ggttttgccc acagtgtggc ctataccaac tcggttgaaa
    10201 atgcactggg gattgaggtg ccgcaacgag cacatactat tcgctcgatt ctgctggaag
    10261 tcgagcggct acacagtcat ttgctcaacc ttggcctctc ctgccatttt gttggttttg
    10321 ataccggctt tatgcaattt ttccgcgtgc gggaaaagtc gatgacgatg gcggaattgc
    10381 tgaccgggtc gcgtaaaacc tacggtctga atctgattgg tggtgttcgc cgcgatattc
    10441 tcaaagagca acgtctgcaa acgctgaaac tggtgcgcga gatgcgcgcc gacgtgtcgg
    10501 agctggtaga gatgctgctt gctacgccga atatggaaca acgcactcag ggcattggca
    10561 ttctcgaccg acaaatcgcc cgtgattata gccctgtagg gccgctgatc cgcgcagtgg
    10621 ttttgcccgt gatttgcgct ttgatcaccc ctacgccgac tacggcaata ttccaaaaac
    10681 actgtttacc tttaccggcg gcgatgtttt ctcccgcgtg atggtccgtg tcaaagagac
    10741 gtttgattcg ctggcaatgc tggaatttgc cctcgacaac atgccggata ccccactgct
    10801 gaccgaaggc tttagctata aacctcacgc attcgcgctg ggctttgttg aagcgccacg
    10861 cggtgaagac gtgcactgga gcatgctcgg tgataaccaa aaattgttcc gctggcgctg
    10921 tcgtgccgcc acctacgcca actggccggt gttgcgttac atgctgcgcg gcaataccgt
    10981 ttctgacgca ccgctgatta tcggtagcct tgatccctgc tactcctgta ccgaccgtgt
    11041 gacgctggta gatgtgcgca agcgccagtc aaaaaccgtg ctgtataaag agatcgaacg
    11101 ctacggcatt gatcgtaacc gttcgccgct gaagtaagga cagaagatgc tgaagttact
    11161 gaaaactatt atgcgcgccg gaaccgcgac ggtgaaatat cccttcgcgc cactggaggt
    11221 cagcccaggc tttcgcggga aaccggacct gatgcccagc caatgtattg cctgcggtgc
    11281 ctgcgcctgt gcttgtccgg caaatgcgct gactatccag accgacgacc agcaaaattc
    11341 gcgcacctgg cagctctatc tggggcgttg tatttactgc ggacgttgtg aagaagtgtg
    11401 cccgaccaga gccatccagc ttaccaataa ctttgaactg accgtcaccc ataaagccga
    11461 tctctatacc cacgcgacgt tccatctaca acgttgcagc cgttgcgaac gcccgtttgc
    11521 cccgcaaaaa accgtcgcac tggctgctga attgttggca cagcaacaaa atgctccaca
    11581 aaaccgcgaa atgttatggg cgcaagcgag cgtctgcccg gagtgcaaac aacgcgcaac
    11641 gctgatcaac gacgatacag atgtaccgct ggtggctaag gagcagctat gagtccagtg
    11701 cttacgcaat atgtcagcca gcccatcacg ctggacgagc aaacgcaaaa gatgaagcgg
    11761 catttgctac aggatatccg tcgctcggct tacgtttatc gcatcgattg cggcggctgc
    11821 aacgcctgtg aaatcgaaat ttttgctgcc atcacccccg tattcgacgc agaacgtttt
    11881 ggcattaagg ttgtttcatc accgcgtcac gccgatattt tgttatttac tggcgcagtc
    11941 acccgggcga tgcgtatgcc tgcacttcgg gcgtatgagt ctgcccccga tcataaaatc
    12001 tgtgtttcct acggcgcgtg cggtgtcggc ggcggtattt tccacgatct ctacagcgtc
    12061 tggggcggca gcgacaccat tgtccccatt gatgtttgga tccccggctg cccgccaaca
    12121 ccggccgcca ccattcacgg tttcgccgtg gcgctcggtt tgctgcaaca gaagattcac
    12181 gcggtggatt atcgcgatcc caccggggtg actatgcaac cgttgtggcc gcagatcccg
    12241 ccatcacagc gtatcgccat tgagcgagaa gcgcggcggc tggcgggcta tcgtcagggg
    12301 cgagaaattt gcgatcggct cctgcgccat ttaagcgacg atcctacagg aaatcgggtt
    12361 aacacctggt tgcgcgatgc cgacgatcca cgtctcaata gtatcgttca gcaactcttt
    12421 cgcgtactcc gggggttaca tgactgaaga gtgcggggaa attgttttct ggacgctgcg
    12481 aaaaaagttt gtcgccagta gcgacgagat gccggaacac agctctcagg taatgtatta
    12541 ctcgctggct atcggtcatc acgttggcgt gattgattgt ctgaatgtcg ccttccgctg
    12601 cccactgacg gaatacgaag attggcttgc actggtcgaa gaggagcaag cccgacgtaa
    12661 gatgctgggg gtgatgactt ttggtgagat tgttattgac gccagccaca ccgccctgtt
    12721 gacccgggca ttcgcgccac tggcagatga cgcgacgtct gtgtggcaag cgcgtagcat
    12781 tcaattcatt catctgttgg atgaaattgt gctggaaccg gctatctatc tgatggccag
    12841 aaaaattgcg tgagaaggat ttctcattaa taaggactgt tgatggctat gtcagacgaa
    12901 gcgatgtttg ccccgccgca aggaataaca attgaagcgg taaacggaat gctcgcggag
    12961 cggttagcgc agaaacacgg caaggcgtct ttattacgcg ccttcatccc gctgccgccg
    13021 ccgttcagcc cggtacaact tattgaactg catgttctca aaagcaactt ctattaccgc
    13081 taccatgatg atggcagcga tgtgacggca acaacagagt atcagggcga gatggtcgat
    13141 tattcgcgtt acgccgtcct tctcggcagt agtggaatgg cggagctacg ctttattcgt
    13201 acccacggca gccgttttac tccccaggat tgcacactgt ttaactggct ggcacgcatt
    13261 atcaccccgg ttctgcaatc atggctcaat gatgaagcac agcaggtggc gctgcgtttg
    13321 ctggagaaag atcgcgatca tcatcgggta ctggtggata tcactaatgc agtgctgtca
    13381 catcttgatc tcgacgatct gatcgctgac gtcgctcgtg agatccatca ttttttcggt
    13441 ctggcttcag tcagtatggt actgggcgat catcgaaaga acgagaagtt cagcctgtgg
    13501 tgcagcgatc tttctgcctc acattgtgcg tgtctgccac acaatatgcc tggcgacagt
    13561 gtattgctga cacaaacgct acaaacccga caaccgacct tgacgcaccg tgcagatgat
    13621 ctgtttctct ggcaacgcga cccgttatta ctcttacttg catctaacgg ctgcgaatct
    13681 gcgctcctta taccgcttac ctttggcaac catacaccgg gtgcattgtt gctggcgcat
    13741 acctcttcca ctctctttag tgaggaaaac tgccagctac tacaacacat agccgatcgc
    13801 atcgctattg ccgttggcaa tgccgatgcc tgccgtagaa tgaccgattt gcaggaaagt
    13861 ttgcagcaag aaaaccacca gcttagcgag cagctccttt cgaatctggg cgtcggtgac
    13921 attatctatc aaagccaggc aatggaagac ctgctccagc aggtagatat tgtggcgaag
    13981 agcgacagta cggtgttgat ttgcggtgaa accggaaccg gcaaagaggt gatcgccaga
    14041 gcgatccatc aacttagccc gcgacacgac aagccgctgg tcaaaatcaa ctgcgctgcc
    14101 atccccgcca gtcttctgga aagtgagtta ttcggtcatg acaaaggggc gtttactggt
    14161 gcgattaata cccatcgtgg tcgttttgaa attgccgatg gcggcacgtt gtttctcgat
    14221 gaaattggcg atctgccgtt agaacttcag cctaaactgc tgcgcgtatt gcaggaacgg
    14281 gagattgagc gtctcggcgg gagtagaacg atcccggtaa atgtcagagt cattgccgcc
    14341 accaaccgtg atttgtggca aatggttgaa gatcgccagt ttcgcagcga tctcttttat
    14401 cgcctgaatg tcttcccact ggaattgcct ccgctgcgcg accgtccgga agatatccct
    14461 cttttagcaa agcatttcac gcaaaaaatg gcgcgccata tgaatcgttc aattgacgcc
    14521 atcccgaccg aggcgctacg ccagttgatg tcgtgggact ggccgggcaa cgtgcgcgag
    14581 ctggaaaacg tgattgagcg agcggtactg ctgactcgcg gtaacagtct gaatttacat
    14641 cttaatgtcc gacaaagccg tttactgccg acgctaaatg aagattcagc gcttcgcagt
    14701 tcaatggcgt agttgctgca cccgacgacg ccagagaatg acgaagaaga acgtcagcgc
    14761 attgttcagg tattgcgaga aaccaatggc attgttgccg ggccccgtgg cgcggcgaca
    14821 cgattaggga tgaagcgcac cacgctgctg tcacgaatgc agcgtctggg aatctcggtt
    14881 cgcgaggtgt tgtaatctgc ttttgcagga gtatgcatga gaaacaaact ctctttcgac
    14941 ttgcagttga gcgccagaaa agcggcaatc gctgaacgga ttgccgccca taaaattgcc
    15001 cgcagtaaag tgtcggtctt tttaatggcg atgtccgctg gcgtgtttat ggcgatgtcc
    15061 gctggcgtgt ttatggcgat cggatttact ttttaccttt ccgttatcgc cgatgccccg
    15121 tcttcatagg cattaaccca tctggtgggc ggcctttgct ttacacttgg ctttattttg
    15181 ctggcggttt gcggcaccag cctgttcacc tcgtcggtaa tgacggtaat ggcaaaaagt
    15241 cggggcgtta ttagttggcg aacttggctg attaacgcac ttctggtggc ctgcggtaat
    15301 ctggcaggta ttgcctgttt cagtttgtta atctggtttt ccgggctggt gatgagtgaa
    15361 aacgcgatgt ggggagtcgc gattttacac tgcgccgagg gcaaaatgca tcatacattt
    15421 actgaatctg tcagcctcgg cattatgtgc aatctgatgg tttgcctggc gctgtggatg
    15481 agttattgcg gacgttcgtt atgcgacaaa atcgtcgcca tgattttgtc catcaccctg
    15541 tttgtcgcca gtggctttga gcactgtatc gccaatttgt ttgtgattcc gttcgccatt
    15601 gccattcgcc atttcgcccc tacctccttc tggcaactgg cgcacagtag cgcagacaat
    15661 tttccggcac tgacggtcag ccattttatt accgccaatc tgctcccggt gatgctgggt
    15721 aatattatcg gcggtgcagt gctggtgagt atttgttatc gggctattta tttacgtcag
    15781 gaatcctgaa aaatcagccc ggcgaaacag ttcgtcgggc tgaagattat tcttgcgcga
    15841 tttgcccatc gggccaggcg tggatcacgg ctttgatcag cgtcgccaat ggaatggcga
    15901 agaatacgcc ccagaatccc cacaaaccac cgaagatcac caccgataaa ataatcacca
    15961 gcggatgcag gttaaccgct tcggagaaca acaccggtac tagcaggttg ccgtccagcg
    16021 cctgaataat cagatacacc gcgaagcagc tccagaattc agtacctgcg ccaaactgga
    16081 atagcgccac gccaaccacc ggaatggtca ccacaaatgc gccaatgtac ggaataagaa
    16141 ccgagaagcc gaccagcact gccagcagca gcgaatagtt cagcccgaag agcaagaacc
    16201 ccagccaggt ggcgatcccc accacgatca tctccagcac tttgccgcgg atatagttgg
    16261 tgatttgttg attcatctcc ttccacacct gtcctgccag cccacggtta cgcggcagca
    16321 cccggcgaac ggcgttcagc atctgctctt tgtctttcag caggaagaag accatcaatg
    16381 gcaccagcac cagatagacg gctatggtca gcaaaccgac cagcgaggcg agggaaattt
    16441 tcaccaccga atcgcccatg gtcagcatcc gactgcgcat attttcggcc attgcatcaa
    16501 taatgcccgc gtccattaac accggatagc ggcgcggcaa cgtggcggca aagtcagaaa
    16561 gcttattgag catccccggc atatcgcgga ttaagtagat gccctgttgc caggcgatag
    16621 gcagtaccac gaacgccatc agtagcaata taccgacaaa aaccaccaat acaatcgacg
    16681 tcgcccagcg gcgggagcag ccaatagatt gcaggcgcac ggttggccat tccagcaaat
    16741 aagccagcac aatagccacc agcaacggag caagcaggcc actaaagaaa aagataatgc
    16801 caaatccggc aactaaaata accagcaagg caatcgcttc cgggtcgcta aaacggcggc
    16861 gataccattg catcaacatt tcgagcataa agaccttccc tgaacctcaa gagcgggatt
    16921 gcgatccgca attgtatcga aatgtcacaa aaaagacttc gctttttatg acggattcag
    16981 gaaactgaaa agtcatttga gtgggctaat cttcgccgtt acactcaaag gcggcgaggt
    17041 gggaacgata tttcacagta tcggtcaaat gactatcttc agaaatacag gatagaggtt
    17101 atgttcaggc agttgaaaaa aaacctggtt gcaaccctca ttgctgctat gaccattggt
    17161 caggtagccc cggcgtttgc cgacagcgca gacaccttgc cggatatggg aacctccgca
    17221 ggaagcacgc tttccattgg tcaggaaatg cagatgggcg actattatgt ccgccagcta
    17281 cgcggcagcg cgccgttaat taatgacccg ctgttaacgc aatatattaa ttcgctgggg
    17341 atgcgtctgg tttcgcatgc caattcggtt aagacaccgt ttcatttttt tctgatcaac
    17401 aacgacgaaa ttaacgcctt tgctttcttt ggcggcaacg tggtgctgca ctctgccctg
    17461 ttccgttatt ccgataacga aagtcaactg gcttcagtta tggcgcacga aatctcccac
    17521 gtcacccaac gtcacctggc gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc
    17581 tgggtcggcg cgttaggttc tattttactg gcgatggcca gtccgcaggc ggggatggcg
    17641 gcgctgaccg gtacactggc gggaacgcgc caggggatga tcagtttcac ccagcaaaat
    17701 gaacaggaag cggaccgcat tggtattcag gtgctgcaac gctcgggatt cgatccacag
    17761 gcgatgccga ccttcctgga aaaattgctc gatcaggcgc gttactcctc gcgcccgccg
    17821 gaaattttat tgactcaccc gttgccggaa agtcgtctgg cagatgcccg caaccgtgct
    17881 aatcagatgc gcccgatggt ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc
    17941 acactgggga tgtataattc cggacgtaac cagctcacca gtgatttgct ggatgaatgg
    18001 gcgaaaggaa acgttcgtca gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg
    18061 gaagccaata aatacgacga ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct
    18121 ggcaacgcat ggtatctcga tctggctacc gatatcgatc ttgggcaaaa caaagccaat
    18181 gaggcaatca atcgcctgaa aaatgcccgc gatttacgca ccaatccggt gttgcagctc
    18241 aacctggcga acgcttatct gcaaggcggt caaccacaag aagcggccaa tattctgaat
    18301 cgctacacct ttaataataa agatgacagc aacggctggg atttgctggc acaggcggaa
    18361 gccgcgctaa ataaccgcga tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc
    18421 ggacgactcg atcaggccat ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc
    18481 agcctgcaac aagcgcgtta cgatgcgcgc atcgaccagt tgcgccagct gcagaaacgc
    18541 tttaagcctt ataccaagat gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa
    18601 aaatctacca taacccacgc tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa
    18661 acggcgtgga gccagaagtg gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc
    18721 gcgatttgct gaaaatgctg gggatgaata gcgcccgtga attgatgcgc cagaaagagg
    18781 atctttataa agaactaaac ctggctgaca gttcgcttag cgaagaggcg ctgattcagg
    18841 caatggtgga taatccgaag ctgatggagc gcccgattat ggtcgcgaat ggcaaagcgc
    18901 ggattggtcg tccaccggag caggtactgg aaatcgtggg ttaagatgtg ctccatcgct
    18961 agttgaagca cattgccgga tgcgacgctt gccacgtctt atccggccta cgaatactat
    19021 ggattaactt cgcgtaggtc aggcgttcgc gccgcatccg acaataaaca ccttatctac
    19081 aacttcagaa tttctttcac aaacggaatg gtcagcttac gttgcgcggt aatcgacgca
    19141 cgatccaact gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc
    19201 aagaaacgcc ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc
    19261 gcctgcaact tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgaga
    19321 cgcgacgcga gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc
    19381 aacaaccgtg ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc
    19441 cacaactcat cgcctgcaat acactcaatg ttgtcgatac agaccagcga caaatgctcc
    19501 ataccgtcga gcacttccgg aacaaaccag gtgcgtttat ccagcgggac atagcccacc
    19561 gcatcgccac gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg
    19621 ccttcgcgtg cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc
    19681 gcggccagta aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga
    19741 agataaagtg gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca
    19801 caaaatcgcg agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc
    19861 ccggtcggta cattatttgt ctgttatatc cgcgtcttct gcgtccagca ccacttcttc
    19921 agggcgcagc acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc
    19981 cagcgccata ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat
    20041 gatcaaaatc acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc
    20101 gatcaaaaca cgaataccgg aagcaccaat gacaccataa agcagcagcg aaacgccgcc
    20161 catcaccggc aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag
    20221 gatagcgaaa atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat
    20281 cacgccaatg ttttctccgt aagtggtatt tggcgtagag ccaaagaagc cggagatcac
    20341 ggtcgacagg ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt
    20401 tttgacgata ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc
    20461 cgctggcaga atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag
    20521 tgcaaaccag tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag
    20581 cgcgtacccc accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag
    20641 cacggaacct aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtt
    20701 tggcgtttgc ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc
    20761 cagaccgatg acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca
    20821 gccggtcccc gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat
    20881 aaagccgccc agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa
    20941 ggcaaagctg gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag
    21001 cgttccaata ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac
    21061 caggacggtt gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa
    21121 aagtggcggt ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt
    21181 atgtgttata ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt
    21241 ttattcttta tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt
    21301 atccgtgctc gttcagtccc tggtcaatcg atgcagtata cagttcgacg tccgggtgcg
    21361 ctttttccag cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc
    21421 tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca
    21481 ttgggtcaac gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg
    21541 gtaccggctc cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg
    21601 gaacgttttc cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg
    21661 taattttctt acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta
    21721 ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag
    21781 cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt
    21841 tgacgagtgg gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca
    21901 caaaaaaatc gacggattat acctcctttc ttcagggcgg caatattatt ttcgttgact
    21961 ttagtcaaaa tgataacagt ttgagataaa gttattttat attcaaatgg ttatgaaaga
    22021 agattattcc atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgcc
    22081 cggggaaaag acgtgcaaaa aggttgtgta aaacagtctc gcaaacgttt gctttccctg
    22141 ttagaattgc gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg
    22201 accgataaaa cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct
    22261 ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt
    22321 ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt
    22381 tccggcactg acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac
    22441 accattggta ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa
    22501 ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg
    22561 gtgatcagcg gcatcgcgga aggttgtctg caatcgggct gttctctggt gggtggcgaa
    22621 acggcagaaa tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg
    22681 ggcgtggtag aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgtgctg
    22741 attgcactcg gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt
    22801 gaagtcagcg gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat
    22861 ctgctggcac cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat
    22921 gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg aaaacattcc gcgcgtattg
    22981 ccagataata cccaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac
    23041 tggctgcaaa cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc
    23101 gtcgggatga ttatcgccct gcctgctccg gaagtggaca aagccctcgc cctgctaaat
    23161 gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc
    23221 gtggttatcg aataatgaat attgtggtgc ttatttccgg caacggaagt aatttacagg
    23281 caattattga cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca
    23341 ataaggccga cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc
    23401 tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg
    23461 acatgtacgc acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt
    23521 ttgtctccca ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc
    23581 ccggattaca cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg
    23641 tgcatttcgt caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg
    23701 tatttgctgg tgatacggaa gatgacatca ccgcccgcgt gcaaacccag gaacacgcta
    23761 tttatccact ggtgattagc tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg
    23821 cgtggctgga tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc
    23881 gtagttaaag cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc
    23941 cagcagtttt tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata
    24001 atatccaggc agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc
    24061 tatacatcga aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg
    24121 cggacaaatc taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc
    24181 ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag
    24241 aacaaggctc caacgctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga
    24301 aagccgatca ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc
    24361 agatcttcct gattaatgaa cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt
    24421 attttaagca gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact
    24481 tagtgcagtt cctgaaagat gattacacct atctggcggt ggaaattatc cgtagcgata
    24541 gcatccgtta cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac
    24601 cgccagaagc gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact
    24661 gccttgatga tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga
    24721 agatgacccg cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt
    24781 tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc
    24841 gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg
    24901 actccatcgt ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg
    24961 tcggcaaagc caatctggtg aacaaaccac tgccgcgttt acgccatatt tggtttgata
    25021 aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc
    25081 cttatcacac ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg
    25141 tactggcgat taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcaa
    25201 tgatccacgc cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt
    25261 tcgacgaaga agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta
    25321 tcttctctgc gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa
    25381 acggtgaagt ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc
    25441 gtctttatac tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc
    25501 gggtatttaa ctttattgaa aacccatacc gcccggtgac atttgattat ttaatggtgt
    25561 caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc
    25621 agcaagggct gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg
    25681 ttgatcgtct gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa
    25741 tgtgttcgct gatccccaat ctggaaggca ttagcgacaa cattcgtgcc atcagtattg
    25801 ttgaccgtta ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg
    25861 tctacctttc ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga
    25921 caccgctgct cgatccgcgc ctgaagcagc gggtgctgga catcatcgac atattgttca
    25981 gcgatacggt caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg
    26041 gcaatcgccg caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac
    26101 aacctgaata accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg
    26161 gtcgatcttg gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg
    26221 cagattattg gccgcctgaa acagcgggtg catctggcgg acggcctggg gccagataat
    26281 atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg
    26341 ctacaagggt tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg
    26401 ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt
    26461 atttccggta atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggaa
    26521 aaaggtcgca aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa
    26581 aatttcgaac ctattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt
    26641 tattttcctg gcggggtcat caataaagag aattttcagc gcgctcgcat ggcggcagca
    26701 caaaaactgg aaactttaac ctggcaattc cgtattcagg gctggaacgt tgcaatgggc
    26761 gcttccggta ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg
    26821 ataattaccc cggaacgtct ggaaaaactg gtaaaagaaa ttttacgtca ccgtaatttc
    26881 gcatcgctga gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg
    26941 gcgattttat gtggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg
    27001 gcgttacgcg aaggcgtact gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt
    27061 agtcgcaccg ccagcagcct cgccaaccag tatcacatag acagcgaaca ggcccgacga
    27121 gtgctggata ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg
    27181 catccgcaac tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat
    27241 atcaaccaca gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg
    27301 ggttttaatc aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg
    27361 attaagctcg acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg
    27421 atacagctat tgcgccttgg cgtattacta aacaatcaac gtcaggcaac caccacaccg
    27481 ccaacattga cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg
    27541 tttagtcaga atgcgctggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc
    27601 gtggctggct ggcggttgaa aattgaagaa gaaagtaccc cagaaatcgc cgcttaatgc
    27661 tttgcgggcc gacgagaaat gtcggcccgc attattcagg cactttcgcg aatgggttcg
    27721 atttcattca gcgtatcaat taacggttgc ggcttaccaa taagataacc ctg
//
LOCUS       DAJTYL010000049        27768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYL010000049 DAJTYL010000000
VERSION     DAJTYL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.049"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            97..483
                     /locus_tag="OO572_002005"
     CDS             97..483
                     /locus_tag="OO572_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661016.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            508..2721
                     /gene="tssI"
                     /locus_tag="OO572_002006"
     CDS             508..2721
                     /gene="tssI"
                     /locus_tag="OO572_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6661017.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2744..3189
                     /locus_tag="OO572_002007"
                     /pseudo
     CDS             2744..3189
                     /locus_tag="OO572_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3211..3618
                     /locus_tag="OO572_002008"
     CDS             3211..3618
                     /locus_tag="OO572_002008"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6661018.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            3615..5903
                     /locus_tag="OO572_002009"
     CDS             3615..5903
                     /locus_tag="OO572_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6661019.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            5900..6889
                     /locus_tag="OO572_002010"
     CDS             5900..6889
                     /locus_tag="OO572_002010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661020.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            6989..7192
                     /locus_tag="OO572_002011"
     CDS             6989..7192
                     /locus_tag="OO572_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661021.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7210..7530
                     /locus_tag="OO572_002012"
     CDS             7210..7530
                     /locus_tag="OO572_002012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661022.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(7596..7828)
                     /locus_tag="OO572_002013"
                     /pseudo
     CDS             complement(7596..7828)
                     /locus_tag="OO572_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(7938..8708)
                     /gene="yafV"
                     /locus_tag="OO572_002014"
     CDS             complement(7938..8708)
                     /gene="yafV"
                     /locus_tag="OO572_002014"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6661023.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8889..9335
                     /gene="ivy"
                     /locus_tag="OO572_002015"
     CDS             8889..9335
                     /gene="ivy"
                     /locus_tag="OO572_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6661024.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9378..11822)
                     /gene="fadE"
                     /locus_tag="OO572_002016"
     CDS             complement(9378..11822)
                     /gene="fadE"
                     /locus_tag="OO572_002016"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6661025.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12062..12640
                     /gene="lpcA"
                     /locus_tag="OO572_002017"
     CDS             12062..12640
                     /gene="lpcA"
                     /locus_tag="OO572_002017"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6661026.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12846..13613
                     /locus_tag="OO572_002018"
     CDS             12846..13613
                     /locus_tag="OO572_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6661027.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13584..14324)
                     /locus_tag="OO572_002019"
     CDS             complement(13584..14324)
                     /locus_tag="OO572_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6661028.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            14708..15229
                     /locus_tag="OO572_002020"
                     /pseudo
     CDS             14708..15229
                     /locus_tag="OO572_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15405..15902
                     /gene="rayT"
                     /locus_tag="OO572_002021"
     CDS             15405..15902
                     /gene="rayT"
                     /locus_tag="OO572_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6661029.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16119..17831)
                     /locus_tag="OO572_002022"
                     /pseudo
     CDS             complement(16119..17831)
                     /locus_tag="OO572_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            17803..18588
                     /gene="lafU"
                     /locus_tag="OO572_002023"
     CDS             17803..18588
                     /gene="lafU"
                     /locus_tag="OO572_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6661030.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            18659..19714
                     /gene="dinB"
                     /locus_tag="OO572_002024"
     CDS             18659..19714
                     /gene="dinB"
                     /locus_tag="OO572_002024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6661031.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            19766..20059
                     /gene="yafN"
                     /locus_tag="OO572_002025"
     CDS             19766..20059
                     /gene="yafN"
                     /locus_tag="OO572_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6661032.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20062..20460
                     /gene="yafO"
                     /locus_tag="OO572_002026"
     CDS             20062..20460
                     /gene="yafO"
                     /locus_tag="OO572_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6661033.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20470..20922
                     /locus_tag="OO572_002027"
     CDS             20470..20922
                     /locus_tag="OO572_002027"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6661034.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21168..22211
                     /locus_tag="OO572_002028"
     CDS             21168..22211
                     /locus_tag="OO572_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6661035.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22273..22887
                     /locus_tag="OO572_002029"
                     /pseudo
     CDS             22273..22887
                     /locus_tag="OO572_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(22944..24401)
                     /gene="pepD"
                     /locus_tag="OO572_002030"
     CDS             complement(22944..24401)
                     /gene="pepD"
                     /locus_tag="OO572_002030"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6661036.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            24663..25121
                     /gene="gpt"
                     /locus_tag="OO572_002031"
     CDS             24663..25121
                     /gene="gpt"
                     /locus_tag="OO572_002031"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6661037.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25213..26457
                     /gene="frsA"
                     /locus_tag="OO572_002032"
     CDS             25213..26457
                     /gene="frsA"
                     /locus_tag="OO572_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6661038.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26515..26916
                     /gene="crl"
                     /locus_tag="OO572_002033"
     CDS             26515..26916
                     /gene="crl"
                     /locus_tag="OO572_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6661039.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(26955..>27768)
                     /locus_tag="OO572_002034"
     CDS             complement(26955..>27768)
                     /locus_tag="OO572_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6661040.1"
                     /translation="AGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAW
                     TDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKK
                     QNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANN
                     IYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGD
                     EDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         6806 a         6975 c         7423 g         6564 t
ORIGIN      
        1 gggaatggag ctttgggaac gataccttaa aaacgcaact ataacgttga atatcataat
       61 gcctgaaact aaatttactt tattgaatgt aaccaaatga aatttttctg tattatagtg
      121 attttccttt caataaatta catagctata gtgaatgttt tcaagtcttc tgcagttgct
      181 caatccacca aatattatga agaaaatgat atacatgatg gaagtaatga catctttttt
      241 atgaagaaat atgggccgcc tgctcatgag ttcgtgaggc cacgatataa atttgttggt
      301 gagtattata tcggcttgaa tcaaacttat cgcataacag atcctgaatt tcaagatgtt
      361 cctattaagg aaatgttctg gaatttgcag ggggatttga atctgacatg ttggtttcat
      421 tataaagacg ggcaatggag agtgattact tatatttttt ggccaccagg ggctagattt
      481 taatccggta ttaaaggagc aaatatcatg agttttgtat ctacgggtaa taaaccagtt
      541 ggaaacggtg gacccgtcat caccacacca ccaatagctg gcgaaagtgg cggtatgagc
      601 acaggttcag ctgtaaccga tgtatctggt gccgccgagg agatggcaga acaggctgcg
      661 gcggacctgt ttggtgcgct gccggaaccg tcaggtctgg ttaaagccgc agtagctgct
      721 gcgcaggctg ccgctgcagc agcagggatc tcggatatgg ctggtgcagt tcaggatgca
      781 gcggcgtcgc ttgcagccgg tgctccgggg gcgcataacg tcacagtaag tggtagtgcg
      841 gtgccgccgc agatgctttt gtttgcaggc atgaatggtt cggaagggct tgggaatctg
      901 ttttcttaca cagtacagct taaaacgctg gacgccctga acctggggta cgtttccccc
      961 gctgctaacc tgccgcttca gctcatggtg ggcaaagacc tgtgcgtcag tatcgaactg
     1021 gacggcggcg gaaaacggta tatcagcggg ctggtgacgg cggcgagggt ggtcggccat
     1081 gagagccgtt cggtaacgta tgaattacgc attgagccat ggctgaagct gctgacccat
     1141 accagcgact acaaagcgtt ccagaacaaa accgtggtgg atattctcga tgaagtactg
     1201 gatgaatatt cctttccggt ggaaaagcgg ctggtggaaa attatccacc ccgcgcctgg
     1261 caggtgcagt atggtgaaac cgacttcgat ttcatccagc gcctgatgca ggaatggggg
     1321 atttactggt ggtttgagca cagcgagaac agccacacgc tggtgctggt ggatgccatc
     1381 aatggtcata aagcgtgccc tgactcgccg ctggtggagt ggcaccagga agggctgaaa
     1441 ctggataagg aatttatcca caccattacg gccagtgaga ggctgcgtac tggaaagtgg
     1501 gtgatggacg attttgattt tatgaagcct cgttcgctgc ttaaaagtac cgtggccagt
     1561 ccgcgtgata ccggtcatgc tgaatacgag cattacgaat ggcctggtga ttacttcact
     1621 acgggcgaag gcgagatgct gacgcgcatt cgtatggaag cgcagcgcag cccaggcagc
     1681 cgggcacatg gggccggtca tatccgtacc ctcatgaccg ggtacacctt tacgttgatg
     1741 aatcacccga cggcagaaat taatcaggaa tatctgctgg tacagaccac tctgttttta
     1801 cgggataatg cgcagcacag cggtcaggat cagcatttta cctatgtcac cacgtttgaa
     1861 ctgcacccca ccagcgaagt ttaccgccca caacgaaccc tgagcaaacc ccacaccaaa
     1921 ggcccgcaga gcgccatcgt caccggcccg gcggggcagg aaatctggac ggataaatat
     1981 ggccgggtga aggtgcagtt tggctgggac cgttacggaa agaatgacga aaacagttcc
     2041 tgctggatcc gcgtcagcta tccgtgggcg ggtaaaggat tcggcatgat ccagatcccg
     2101 cgtattggcc aggaagtact ggtggatttc aaaaacggcg atccggattt gcccatcatc
     2161 gtggggcgca cctataacca ggacaccatg ccgccgtggg ggctgccggg catggcgtcg
     2221 cagagtggga tattcagcca ctcattgcag ggagggccga ctaacggaaa tatgctgcgc
     2281 ttcgatgata aaacgggtgc ggaagaggtg aagttccatg cggaaaaaga cctcaatacc
     2341 acggtaaaaa acaatgaaac ccacacggtc atggtcgatc gcataaaaac cattgtcaaa
     2401 aatgaaacca tcagcgtggg agaagaccgt aatgccacca tcacaaaaaa tgatggtctt
     2461 tccgtcaaac tggctcaaac catcaatgtc ggaacgacat atcgtctgga tgttggcgat
     2521 caatttaccc ttcgttgcgg caatgcagcg cttgtattac acaaggatgg ctcgattgag
     2581 ttttgcggaa aacaactgtt actgcacacc agcgatgtta tgcaactcat tggcagtggc
     2641 attgatatga atccggatgg cggcacagcc atgactgcgg atgatattgc cccgttacct
     2701 acccccgacg tttctgaata aaaaatataa aaggaatttt tctatgacca gcctttaccg
     2761 tattcaggag ggctgctttg ccctgcctga aacttttctt gatcgcaccg tcaatatctt
     2821 cgtcccatcg ggcaatgaaa gggcaactcc cagtctgaat attttccgcg atacgctcag
     2881 gccagatgaa aatctgacca cctatattga ccgccagata gccttgatga aaaaaaaaac
     2941 ctaggccagc accgggtact ttcgcgggga acggcactgg cgggaactgg cgaagctgcc
     3001 atgacaggtg aacagattct cgcaactcat cgttctggaa aaacagaagt gtaccagcgt
     3061 caggccgggt ttatcaccgg gccagcaaag gtactaatgt tgacgctgac cactcagcgt
     3121 ccttttgatg atcacaccga ccagctatgg actgcatggt tgactagttt tcagcctgct
     3181 aaaagctaaa aaaaacaggg aggttttgtc atgtatgaag ccgcacgggt ggatgacccc
     3241 atctaccaca ccagcgccct tgccgggttt ttgatagggg ccatcatagg tatcgccatt
     3301 attgccgtcg ccagctttgc tttttttacc tgcggctttt tggccgggct gattttgagt
     3361 tttctggctg atcagatagc ttccggagtc ctgcaactgg gtgaggctat tgggcgctcg
     3421 atacattcca cagtgggaaa aattctgacc ggttctgata atgtcagcac caacagtcgc
     3481 ccggccgccc gggccgtact gagtacggtg atatgcgaag accattcccc ggaaatccgc
     3541 attgcccaag gttccggcaa catctacatc aacagccagc tgcccgtaag gatgaccaca
     3601 ctgagtgtga tgcagtgatt gaggacggct cccccaatgt ctttctcggc ggcggtacgc
     3661 agacggtact ggaaatcagc ccggaaatcc ctgactggct gcgtcaggtg gtggatgtgc
     3721 tgtatgtggt cgccggaatg cttggcgggc tggctggcgc ctggcgtcag gcggcaaaga
     3781 tggggtcgaa atttggtaca aaatgtgcgg caaaatttat tggtggcgaa atggtcggga
     3841 tggccatctc tgacacagtc atgggattgt tcagcaatcc ggtagatgtc accactgggc
     3901 aaaaaatttt gctgccggag acggatttta ccctgccagg acgcctgcct gtcacctgtt
     3961 cgcgctttta cgccagccac ctggaaactg aaggattgct gggcagagga tggcggctga
     4021 actgggagat aaacctgcgt gaggatgaaa catatatcac cttcatcggt gtacagggtc
     4081 gggaactgag ttatccgaaa gagatgctga taccgggcca ccagatattc gacccggaag
     4141 agcagtttta cctgagccgt ctgcatgacg gatgctatgt gctgcattac accgactgca
     4201 gctattacgt gtttgatgaa tttgatgacc acggcgtcgc gccgttactt tttatggaga
     4261 cgccgtaccg gcagcgtatt gctttcgggc gtgagaacgg caggctggtg cgggtggcct
     4321 ccagcagcgg acaccatctg ttattgcacc gcacgatgac gcaagcaggg gaacgcctgt
     4381 cgcatattga acttttgaag ggcgggcgtc ccggtaacct ggtggaatat cgttacgacg
     4441 ataacgggca actgactggc gtggtgaacc gggctggtgt gacggtgcgc cagtttgcct
     4501 atgaaaatgg cctgatgaca gagcatcgca acgcgacggg ctttacctgt acctaccact
     4561 gggaagagat tgaaggtttc ccgcgcgtgg tggaacacac gaccagcgac ggcgaggatt
     4621 accggttcca ctatgatttt gctggcgggc agacggtggt aaccggcagg ccggagcaga
     4681 agtggcagtg gtggtttgat gaagagacgt atgtgactgc gcaccggacg ccgggcggcg
     4741 gaatgtaccg cttcacgtac aacgaaaacc attttccggt agcggtggaa ctccccggcg
     4801 aacgcagggt aacgctggaa tacgacaccc tgagccgggt ggtgaaagag acggacccgg
     4861 caggccgggt gacacagacg cagtggaacg gcagttttgc tgaaatcacg cgccgggcgc
     4921 tggatgacga ccacgtatgg aaggcggatt acaacgaaca cggccaggtg atacgggaga
     4981 cggacccgga agggcgcgtc acgcggtacg ggtatgatga tcaggggctg ccggagacgg
     5041 tctgccaccc cggtaaacag caggaccgtt acacctggaa cgcgctgggg ttactgagca
     5101 gccaccgtcg catcacgggc agtgtgcagt cctggcagta tacgcagcgc gggatgctgg
     5161 cgcgccatac ggatgaagag aaacgggaga cgcgctggca gtacaccccg gaagggctgg
     5221 ttgccagtct gagcaacggc aacggggctc agtaccggtt cagctacgac ggcgacggac
     5281 ggctgacggg agaacagcgc ccggacggcc tgataaggat gtttgcgctg aatgcggacg
     5341 ggtttccggt catcatcccg acgcagggca cagagggcgg ggtgcgcaat gaacagcagg
     5401 agcgtgatgc gctgggccgc ctgctgcgca gtgacacgca gcacagtaca cggaccttca
     5461 gctacaaccg tcttgaccag ataacggaag tgacgctcac ccccacggaa gaaggggaac
     5521 ggctgcacca catgcaggcg gacacggtgc gttttgcgta tgaccgcagc gggtggctga
     5581 cggcagagca cagcgtgcac ggcagtatca aataccggcg cgatgcgctg ggcaatccca
     5641 ccgatatcac gttgccggac gggcagcatc tcagtcatct ttactacggc agcgggcatc
     5701 tgctccagac ggcactggac ggtatcacgg tcagtgaata tgagcgtgac agcctgcacc
     5761 gtcaggtgat acgcacgcag gggaaactgg cgacgttcag cggctacaac gccgacaacc
     5821 ggttaagctg gcagcgcagc ctgccaggtg gcagccagaa tccacagcag gcggtactcc
     5881 cccggcgcag aacgacagcg tgacggcgcg ggactatatc tggaacgcag acggtgaagt
     5941 cggcggcatc aatgacaaac tgcgcggttg cctggtgttc agctatgacc gcagcggctg
     6001 gctgaccagc cgcacggggc agatgtatga ccatgaccat tattattacg acaaagcggg
     6061 caacctgctg acggatgagt atcagggagc ggtgatggat aaccgcctgc cgggttacgg
     6121 gcgtgaccgc taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcagct
     6181 ggagtggaac gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg agacgcggta
     6241 tcagtacgat gcgctgggca ggcgcatcag caaggcgacc agcaatctcc ataccgaccg
     6301 gggagaacga agccggacaa cgtttgtgtg ggaagggttc cggctgttac aggagacaac
     6361 gtggcaggga aagcggacat acctgtacga tgcggaacag ccgtacacgc cggtggctgc
     6421 catcacgggg cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac
     6481 ggtacacgag gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt
     6541 tggtgaaaac cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct
     6601 gccggggcag tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc
     6661 gccggagtgc gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt
     6721 atatgcttat gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg
     6781 aatagattca aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca
     6841 gccaaagagt ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga
     6901 tgggcgttta acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga
     6961 ttttttctca aaaaaacatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa
     7021 aaataatcag atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc
     7081 aagcaagaaa attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga
     7141 ggtgttagtt catgggcaaa ttcttggaaa taaagttagc cgatgtaatt agttagtgga
     7201 gaggaagttg tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc
     7261 catgtgctaa aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat
     7321 tgtttagatt gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg
     7381 agtccagaag agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga
     7441 gctaatttga atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat
     7501 aaaaacggca atgaaataga gtctgactaa attttattga tatagcaacg ataataaccg
     7561 gaaatttctt ccagatcttt ccggcttttt attatttaac tttcgttcag cacactaaac
     7621 atcccgtctc gcatcccctt aactggctgt ttaacctgat aaagttccct gcgcagttcc
     7681 tgtacttcgt tgttatccca agcaggatca aacatccctc cgagatcttc acggtaacgc
     7741 ccgtgccgat ctcaaatccc gcttttctca gccatttggc gctgattttg atcaccggcg
     7801 tgtcgctgcg gttgggcaga taacccacgg tacagcgacg ctgtgttttg gaaatggtga
     7861 cttctggcgt aatattgtgt ttaagttagg ggctttcgta atctagccca gcccccggcc
     7921 ctaaacacaa cccccactca ccacaaccta aactcatccg catcccgcca tgccggaaac
     7981 ttttctctat attcccgcaa tgccaccatc gacagctccg catcaatacg cgttgcctta
     8041 tgcgcgtcgg cggtagcgat aatctcgcct tgcggattaa tcacccggct gtcaccgcga
     8101 taatggcagc cgttgccatc gctgccgacg cgattgcatc ccgccacata cgcctgattc
     8161 tcaatcgcgc gggccgtcag caatgcctgc cagtgcagag agcgcggagc tggccagttg
     8221 gcgacgtaca gggcgaggtc ataatcgttg agattgcgcg accacactgg aaaacgtaag
     8281 tcgtagcaca ccagcggcaa aatacgccag ccgcgccatt ccacaatcac tcgcgcattg
     8341 cccgctttat aatgcagatg ctcatctgcc atgcggaaca gatgacgctt atcataaaaa
     8401 tgtaccgtgc cgcccggctc aaccagcaaa aagcggttaa ccgaaccaga ctccgtttgt
     8461 aatgcaacac tgcctgcaat cagcgcattg cactgctgcg ccttagctgt catccagttc
     8521 actacgtcat cttgtgcaag cgacgaagct gccgcttcca tggcaaagcc gctggtaaac
     8581 atctccggta gaacgatcac atcgcgcccg gtaatacctt ccagttgacg atcaaaatgg
     8641 cgcaggttgg caggaccatc cacccacacc aacggttgct gcaaaagcgt aatcttcaaa
     8701 ctaggcacgg tgtacaactc ctttatgcga agggttttat aactttaaca ccttatcagg
     8761 cagttgcctt agcgcagaat aaattgataa caaatgctga tattggaaat atctgatttg
     8821 caaattatcg tgttatcgct aggctttagg aggttaataa catgggcagg ataagctcgg
     8881 gaggaatgat gtttaaggca ataacgacag tcgccgcact ggtcatcgcc accagtgcaa
     8941 tggcgcagga tgatttaacc attagcagcc ttgcaaaggg cgaaaccacc aaagctgcgt
     9001 ttaatcagat ggtgcaaggg cataagctac ctgcatgggt gatgaaaggc ggtacttata
     9061 ctcccgcaca aaccgtaacg ttgggagatg agacgtatca ggtgatgagc gcgtgcaaac
     9121 cgcatgactg tggctcgcaa cgtatcgctg tgatgtggtc cgagaaatct aatcagatga
     9181 cggggctgtt ctcggctatt gatgagaaaa cgtcgcaaga gaaactcacc tggctgaatg
     9241 tgaacgatgc gctttcgatt gatggtaaaa cggtgctgtt cgcggcgttg accggcagcc
     9301 tggaaaacca tccggatggc tttaatttta aataattagc ggataaagaa acggagcctt
     9361 tcggctccgt tattcaatta cgcggcttca actttccgca ctttctccgg caactttacc
     9421 ggcttcgtcg ccagctcttc cggatcaaag tcatcaacgt taatactgcg cagacggctt
     9481 tcttccgctt tcaccagaat agcggcttca tctttatcaa tcagcccctt cgtcagcgcg
     9541 ttgtgcgcca gttcatccag acgggtaaat ggcagatttt tacccagctc tttacagatc
     9601 cgctgatgaa tcgggtcggc ggcaatcaca tccaccaacg cctcttccag taaaccaacc
     9661 gggttatgct cgcttggcgt caggtactga ccgcgaccaa tacgtgaacg agtggcgttc
     9721 ggcacttgta aaatcttcgc cattttatga tccagcttgt cagacggtgc cagataatga
     9781 cgtccggtcg ggaagatcac cacattcagc agcccggcaa ccacgcggtt cgggaagttt
     9841 tgcagtaaat tgtccattgc ctgttctgcc tgatacagcg catcctgtac gccccagtgc
     9901 accagcggca gatcggcttc attacggcct tcgtcgtcat aacgcttcag aacggcagag
     9961 gcgaggtaaa gctggcttaa aatatccccc agacgtgccg agatacgctc acgacgtttc
    10021 aggctgccgc ccagcagtgc catcgagaca tcagaaagca gggcgaggtt ggcgctcagg
    10081 cggttcaggt gctgatagta gcgtttagtg gcatcgccgg ttggcgtgct gctggttaaa
    10141 ccgcgcgtca ggcccagcca gaagctgcga actttgttgc taccgacgtg accgatatgt
    10201 ttgaacaaca gtttatcgaa cgcgttgacg tcattgttct tcgccgcttc catctcttcc
    10261 agcacgtacg gatggcaacg aatcgctcct tgtccgaaga tcatcatgct gcgggtcaga
    10321 atgttagccc cttcaacggt gatggcaatc ggtgcgccct ggtaagcacg cgccaggaag
    10381 ttgctttgcc cgagcataat gcctttaccg ccggtaatat ccatcgcatc aataatcgac
    10441 tgctgcccgc ggtgggtaca gtgatactta acgatagccg acagcacggc aggtttttcg
    10501 ccgagcataa tgccgtaggt aatcagcgat gccgcagcat ccattacata agcgttcccg
    10561 gcaatacgcg ccagcggctc ttcaatccct tccatcttac cgatagagat tttgaactga
    10621 cggcgaatgt gagaatacgc gcccgttgcc agcgctaccg atttcacgcc gccggttgag
    10681 ttggaaggca gggtgatgcc gcggcctacc gagaggcact ccaccagcat ccgccagcct
    10741 tgcccggcca ttttcggccc gccgatgatg taatcgatcg gcacgaagac atctttaccg
    10801 cgcgtcggtc cgttctggaa cggtacgttc agcgggaagt ggcgacgacc aatttccacg
    10861 cccggcgtgg tggttgggat cagcgcacag gtaatgccta aatcttctgc accgccgagt
    10921 aatttttccg ggtcggagag tttaaacgcc agcccaagca cggtcgcaat cggtgccagc
    10981 gtaatgtagc gtttgttcca ggtcagacgc atccccagca cctgctggcc ctgccattcg
    11041 cccatgcaga caatcccggt gtccggaatc gcgcccgcat cggaacccgc ttccgggctg
    11101 gtcagtgcaa agcaggggat ctcctgacca cgcgccagac gcggcagata gtgatctttc
    11161 tgctcgtcag tgccgtaatg ttgcaacagt tcgcccgggc ctaatgagtt tggcacgccg
    11221 acggtaatcg ccaggatccc gctcacgcca gagagttttt gcagcacacg agactgggca
    11281 taagctgaga actccagtcc gccgtactct tttttgataa tcatcgcgaa gaagcgatgc
    11341 tctttaaggt acgcccacaa ctccggtggc agatccgcca gctcatgagt aatctggaaa
    11401 tcattcgcca tccggcaggc ttcttctacc gggccgtcga gaaacgcttg ctcttcagcg
    11461 gtcaggcgcg gctgcggata gttatgcagc tttttccagt ccggcttgcc ctggaacaag
    11521 tcgccctccc accaggtggt gcccgcatca atcgcttctt tctcagtgcg cgacatcggc
    11581 ggcatcacct tacggaaacc gcgaaatacc ggcgcggaaa tcatcgactt acgcataggc
    11641 gcaaagttaa atggcacgag gataatggtc agaggcacca gtacccacgc cgaccacaga
    11701 ccagcaacgc cgagggcggc tgtccaggcg agcaaaatca gactgctgat aaataagctc
    11761 acgcggtgat agaacaacgc gccgagcagg acaaccgtag cgagaatact caaaatcatc
    11821 ataacgaaaa gccccttact tgtaggaggt ctgaccactt gtgatgatat ggttgtagtg
    11881 gatgtaaaaa catttagcaa tgtgtttaca atataattac aacaaagctc acattgttgc
    11941 tgtttttatc cgcacttcag gtcaaaaagt cctggtcata gcacctgccc gtacttctcg
    12001 cttttggcgg tatccggtac actgcatttt gtctattaca tttatgctga aggatatcct
    12061 catgtaccag gatcttattc gtaacgaact gaacgaagcg gcggaaacgc tggctaactt
    12121 tttaaaagat gacgccaata ttcacgccat tcagcgcgcg gcggtcctgt tagcagacag
    12181 ctttaaagcc ggtggcaaag tgctttcctg cggcaacggc ggttcccatt gcgacgctat
    12241 gcactttgcc gaagagttga ccggtcgcta ccgtgaaaac cgtccgggct acccggcgat
    12301 tgctatttct gacgttagtc atatttcctg cgtcggtaat gatttcggtt tcaatgatat
    12361 tttctcccgc tacgttgaag cggtaggtcg cgaaggcgat gtactgctgg ggatctccac
    12421 ctccggtaac tctgcaaacg tgatcaaagc gatcgcagcg gcgcgtgaga aggggatgaa
    12481 agtgatcacc ctgaccggta aagacggcgg taaaatggct ggcacggcgg atatcgaaat
    12541 tcgcgttccg cactttggtt atgccgaccg cattcaggag attcacatta aagtgatcca
    12601 tatcctgatc cagttaattg aaaaagagat ggttaagtaa gtctggcgta ggccggataa
    12661 ggcgtttacg ccgcatccgg cacttgtgcg ctgatgcctg atgcgatgct gacgcgtctt
    12721 atcatgccta caaatctgta cccgaaccgt aggccgaata attcgtttac gccgcatccg
    12781 acctgaaaat tcttaaatca atcttcgccg gggtccatgc gctcccgctg ttgtggaggt
    12841 tacccatgtg cgaattgctc gggatgagcg ccaacgtccc taccgatatc tgctttagtt
    12901 tcaccggact tgtacagcgt ggtggtggaa ccgggccaca taaagatggc tggggcatta
    12961 ccttttacga aggtaaaggc tgtcgcacat ttaaagatcc acaacccagc tttaattccc
    13021 ccatcgccaa acttgtccag gactacccga taaaatcctg ttcggtggtg gctcatattc
    13081 gccaggctaa tcggggcgag gtggcgctgg aaaatactca cccatttacc cgcgagttat
    13141 gggggcgtaa ctggacttat gcccataacg gacaactgac gggctacaaa tcactggaaa
    13201 ccggcaactt ccgcccggtc ggtgaaaccg acagcgaaaa agccttttgc tggctgctgc
    13261 ataaattaac gcagcgttac ccgcgcacgc cgggcaacat ggcggcagtg tttaaatata
    13321 tcgcctcact ggcggatgaa ctgcgggcga agggcgtttt caacatgctg ctttcggacg
    13381 ggcgctatgt aatggcgtat tgctcgacta atttacactg gatcacccgc cgcgcgccgt
    13441 ttggcgtggc aacgttgctg gatcaggatg tggaaatcga cttcagctcg cagaccacac
    13501 cgaatgatgt ggtcacggtg attgcgacac agccgctgac gggcaatgaa acctggcaaa
    13561 aaattatgcc aggcgaatgg cgcttatttt gcctcgggga gcgtgtagtt tgatgccagt
    13621 tgtggctgca ccacttcgtg gcttaacggc ttgctgacca cgtaacggcc attggccaca
    13681 gaaacggtcg gtggcttacg ggtttgctca aagtagtcgt agcccggctt cagctgctcc
    13741 cagaagtcct taaagttgga atatttatgg cgcttcatat tggcgtcggt catgcggaat
    13801 gggtaaatac tcacctgcac gctcggctga ccaaacacca gcgcaccagt aacgaactgg
    13861 aagatctcat caataccctg attggtcatt gcgtagcagc cgatggatac acaatcgccg
    13921 tggatcatca ggtatttccc ttcataacca tgcgcacggt cataagcatt ggggaatcca
    13981 atattaatcg ctttgtagta acggctgtct ggttttaact gattacgctg gacgctataa
    14041 aacccttccg ggcttttgaa atcgccctga cgctgttttg gccctaagcc gccggaatat
    14101 ttacagattt tataactgtc gagcagttga tattgctcgc ccattttgac gtaaagatcg
    14161 agcgtacgtt cttccttgaa gatctggatg tagacagggg atcccatcaa ctgctgctta
    14221 tactctttgc tcactggcgt ggtcgagcta ctgctgccca gcaaaccggc aaacgaaacg
    14281 cacgggatca acaacatcgc aagaattaat gcgattttac gcatactgct tattccttga
    14341 taaaacggtt acacacgcca ggacggcaaa atggatccca aatcggaata gtctggattt
    14401 ggaaggctca cattatcacc aaaagagttt tacgcaagcc tgtcggcgct gggtttacaa
    14461 atttagcaca ggggcagttt tcaatagtcc gaaaagcgca tgtatcattt atttcctaat
    14521 attatgaaat ctcaatagta atatgcaaat aagcaattaa taatcccgtt tactcctccg
    14581 gtcttttaaa attctcacct tcattcagcc ggtttatgtc tttcatgtct tcgttcttgc
    14641 tcggcagatt tttgcatcca ggcgtgtttt cgttatgcgt tttgctccct ttactcgcca
    14701 gcgccaccac aaccgggagc agataaaaca gcatccagaa tggtttcctg cgccgctcaa
    14761 ggcgagtgac agacgctggc aggcgctggc ggaaaacaac cactttttaa gcagcgatca
    14821 cctgcataac atcaccgaag tggcgattca ccgcctggag cagcagcttg gcaagcctta
    14881 cgtctggggc ggcacgcggc ccgatcaagg cttcgattgt agtgggttag ttttttatgc
    14941 ctacaacaag atccttgagg cgaaactccc gcgcacggcc aatgagatgt accactaccg
    15001 tcgggcaacg attgtggcga ataacgacct gcgccgggga gatttgctat ttttccatat
    15061 ccacagccgc gagattgccg accatatggg ggtgtatctg ggcgacggtc agtttattga
    15121 gtcactgcgc accggggaaa ccattcgggt aagccggtta gccgaacctt tctggcagga
    15181 ccatttttta ggcgcgcgca ggattttgac ggaagagatg attttgtagg ccggataagg
    15241 cgtttacgcc gcatccggcg gttgtgcacg gatgcctgat gcgacgctga cgcgtcttat
    15301 catgcctaca aatctgtgcg tttttgcgag ccgctttccc gatataacac agcgtcattg
    15361 tcggcctttc cttttcctct taccgttgag aaaaaggagt cgctatgtcg gaatatcgtc
    15421 gttattacat caagggggga acatggtttt tcacggtaaa tttacgcaat cgtcgaagcc
    15481 atcttctgac cacccagttc cagacgctcc gtaacgccat tattaacgtt aagcgagaca
    15541 gaccttttga aattaacgcc tgggtcgttt tgccggagca tatgcattgc atatggacat
    15601 tacccgaaag cgatgatgat ttttcctctc gctggcggga gattaaaaag cagttcaccc
    15661 atgcttgcgg attgaaaaat atctggcaac cgcgtttttg ggagcatgcc attcgcaata
    15721 caaaagatta tcgccatcat gttgattata tttatataaa tccagagaaa catggctggg
    15781 taaagcaggt gagtgattgg ccattctcaa cgttccatcg cgatgtcgcg cgagggttat
    15841 atcccatcga ttgggcggga gacataacgg atttaagtgc cggggagcgt atcattttat
    15901 aactgtgcgc tggtgccgga tgcgcttacg cttatccggc ctacaaatcg tgctttaacg
    15961 gtaggccgga taaggcgttc acgccgcatc cggcagttat gcgcagatgc ctgatgcgac
    16021 gctggcgcgt cttatcatgc ctaaaaactt gtgcctgaac ggtaggccgg ataaggcgtt
    16081 tacgccgcat ccggcaattg tgcaccgatg ccggattctt acatcaacgc ccccataatc
    16141 ttcaactcca gctcatccgg cacttcgtta tacgacagca catgcagccc cggcgcaaac
    16201 aaccttgcat aacgcggcag caaagggcgt agctgcggcg gcaccagcag caccggatct
    16261 ttccccgccg ctttcatctg ctctttcacc tgtggcatcg tgctctggaa ctggttgagc
    16321 atattcgggt caaccggcac gctgtcgagc atcactttgc cggcctgttg cgcctgattc
    16381 actacgttgg tcagcagatt ttccagctca ttattcagcg tatacaccgt cagctcctgc
    16441 ttgcgaacga acggatgagt aatgctgcgc cgcagcgcca ggcgcacatc ggccgccagc
    16501 agaatatgat ctttagtcac cgtgctactg gcgaccagca cggtggcgat ggtgacgata
    16561 tcgcgcaggg aaacgccttc ggtcagcagc gcccggtaca ctttcagcaa ttggctgtaa
    16621 ttgagcgccg cgcttaaatc ctccgccagg cgcggtgcca tcgacgacaa acggttatgc
    16681 aactgcgtga tgtcatcata gttaaacaaa tcaggaatat agctgcgcac aatcttgttc
    16741 acatgggtgg caatcacact ggcgctgtcg atcacctgat accccatatt cagagccttc
    16801 gctttctgcg caggctggat ccaggtgacc ggtatcccgt acgccgggtc gttccccagc
    16861 acgccgtcaa tctcgccgta ggtttcgctg gagggcagcg ccatcagttt atccgccgga
    16921 atatccgctt catcagcctc aatgccgttg atgaaaatag cgtactggct gggcttgagg
    16981 cggaagtttt cccgaatgcg gatctccggc agcagcacgc cgttaccgtc ggaaatcacc
    17041 tgccgaacgc cgcgaatccg ctgggtgagc gggttgccct gcgctttgtc caccagcgcc
    17101 accagcttgt agccgaggct taagctgatg ggctcgatca gcggaatagt ttcccaactg
    17161 acctgttgtt cgctggtttc agtgatggtg cgggtcagcg tttcgaggct tttctcttcc
    17221 gcctccgccg cctgcgggcg tttgctcatc cgccagccgg taaagccaag cagggcgctg
    17281 aacagcaaaa acggcagatg cggcatcccc ggcaccaccg ccagcacgaa catgatcccg
    17341 gtggcggtgt agagcaccga cgggcttgcc agcaactggt ggcgcacgtc atgggcgata
    17401 tcgccgttgt cgctgacgcg ggtgacgata atcgccgccg cggtggagag cagcagggaa
    17461 gggatctgcg ccaccaggcc gtcgccgatg gtcatcagca cgtattgctg gaaggcggcg
    17521 tcggcgctca ggttgtattt gaagatgccg atacagacgc cgccgctcag gttgatcgcc
    17581 agaatcatca tcccggcgat ggcgtctccg cgtacaaact tcgatgcccc gtccatcgcg
    17641 ccgtagaaat cggcctcgct ggcaacatct ttacgccggg tttgcgcctg cgcctggttg
    17701 atcaatccgg cgttaagatc ggcgtcaatc gccatctgtt tgccgggcat cgcgtccagg
    17761 gtaaagcggg cagaaacctc ggaaatacgc tcggcctctt tggtgacgac gataaagttg
    17821 attgtcaaca gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct gcacgggcag
    17881 tcgattttta acggtggtgg gctgtcgccg ttaaataaaa tcagcccgtc tcatccgcca
    17941 aaacctgcca ccgtggcggt gccggaagag acggagaaaa aagcgcgtga tgtgaatgag
    18001 aaaacggcgc tgctgaagaa gaaatccgcc accgagcttg gcgagttggc aaccagtatc
    18061 aacaccattg cccgtgatgc gcatatggaa gcgaatctgg agatggagat tgttccccag
    18121 ggattacgcg tgctgattaa agacgaccag aatcgcaata tgttcgaacg cggcagcgcg
    18181 cagattatgc cgttctttaa aactttgctg gtggagctcg cgccagtgtt cgactcgctc
    18241 tataacaaaa ttattattac cgggcatacc gatgcgatgg cctacaaaaa caatatctac
    18301 aacaactgga acctttcggg cgatcgcgcg ctttcagctc gtcgggtgct ggaagaggcc
    18361 ggaatgccgg aagataaagt gatgcaggta agcgcaatgg cggaccagat gctgctggac
    18421 gcgaagaatc cgcaaagcgc gggcaatcgg cgtattgaga ttatggtgct gaccaaaagt
    18481 gcgtccgata cgctgtatca atactttggt cagcatgggg ataaagtggt gcagccgctg
    18541 gtgcaaaagc tggataagca gcaggtgctt tcgcagcgaa tgcgctaaat gctgaatctt
    18601 tacgcatttc tcaaaacctg aaatcactgt atactttacc agtgttgaga ggtgagcaat
    18661 gcgtaaaatc attcatgtgg atatggactg ctttttcgca gcggtggaga tgcgcgacaa
    18721 tcccgctctg cgcgatatcc ctattgctat tggcggcagc cgcgaacgtc ggggggtgat
    18781 cagcaccgcc aattatcccg cgcgtaaatt tggcgtacgt agcgctatgc ctactgggat
    18841 ggcgctcaaa ttatgcccac atctcacctt gcttccgggg cgctttgacg cctacaaaga
    18901 agcctcaaat catatccgtg aaatcttctc gcgctacacc tcgcgcattg aaccgttgtc
    18961 actggatgag gcttatctcg atgtcaccga tagcgtccat tgccacggtt ctgcgaccct
    19021 catcgcccag gaaatccgcc agacgatctt caacgagctg caactgacgg catctgcggg
    19081 cgtggcacca gtaaagtttc tcgccaaaat cgcctccgac atgaataaac ccaacggtca
    19141 gtttgtgatt acgccggcag aagttccggc atttttacaa accttaccac tggaaaaaat
    19201 ccccggcgtc ggcaaagtct cggcggcaaa actggaagcg atggggctgc ggacctgcgg
    19261 tgatgtacaa aagtgtgatc tggtgattct gcttaaacgc tttggcaaat ttggccgcat
    19321 tttgtgggag cgtagtcagg ggattgacga gcgcgacgtt aacagcgaac ggttacgaaa
    19381 atccgtcggc gtggaacgca cgatggcgga agatattcat cactggtctg aatgtgaagc
    19441 gattatcgag cggctgtatc cggaacttga acgccgtctg gcaaaggtaa aacctgattt
    19501 actgattgct cgccaggggg tgaaattaaa gttcgacgat tttcagcaga ccacccagga
    19561 gcacgtctgg ccgcggctga ataaagctga cttaatcgcc accgcgcgta aaacctggga
    19621 tgaacgccgc ggcgggcgcg gtgtgcgtct ggtggggctg catgtgacgt tgcttgaccc
    19681 gcaaatggaa agacaactgg tgctgggatt atgatgtata ctattatgta tattctggtg
    19741 tgcattatta tgagggtatc actgtatgca tcgaattctc gctgaaaaat cggtcaatat
    19801 cactgagtta cgtaaaaacc cagctaaata ctttattgat caaccagttg cggttctttc
    19861 taataatcgc cccgcaggat atctcttaag tgccagcgca ttcgaagcgt taatggacat
    19921 gcttgctgaa caagaggaga aaaagcccat aaaggcgcgc tttcgtccaa gtgctgcaag
    19981 attagaggaa attacacgcc gcgctaaaca atatcttaat gatatgacgg atgatgattt
    20041 caatgacttt aaggaataag gatgcgggta ttcaaaacaa aacttattcg cctgcaactt
    20101 acagcagagg aacttgatgc gttaacggcg gattttattt cctataagcg tgacggtgtt
    20161 ttgccagata tatttggtcg cgatgcactc tacgacgact cgtttacctg gccattaatc
    20221 aaatttgagc gagttgctca tattcatctg gcaaatgtga ataatccatt tccgccacag
    20281 ttgcgccaat tcagcagaac gaatgacgaa gcgcatttgg tatattgtca gggtgcgttt
    20341 gatgagcaag catggttgct cattgccatt ctgaagcctg aacctcataa actggctcga
    20401 gataacaacc aaatgcataa aattggaaaa atggcagaag cgtttcgcat gcgtttttga
    20461 atttatatta tgaataacat acaaataaga gattatcagc ctggcgattt tcagcaacta
    20521 tgcgctattt tcattagagc ggttacgatg accgtcagtc agcattattc actacaacaa
    20581 attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc gaaatcacaa
    20641 gtgcgggttg cagttattaa tgcaaaactg gtcggcttta ttacctgcgt tgaacactat
    20701 atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag cgctttgtta
    20761 aaacctttta ttaagtctga atccgaactt acggtggacg caagcataac cgcaaaaccc
    20821 ttttttgaac gttatggttt tcagacagtt aagcagcagc gcgttgaatg ccggggagcg
    20881 tggtttatta atttttatat gcgttataaa ccgcaacatt aaatccagct tgcaatgaaa
    20941 ataacgcccg cctagtatgt gcgcgttttc ttattccaca aactgcaagg aggtaaatca
    21001 tgacaaatcc tttatcaatg actctttgca gacctttcca ggattaattc tttttttctt
    21061 gccctggatt cgtctgccat ttcctgattt tttaattgtt actctcaata attcaagtcg
    21121 cagcacatgc aacttgaagt atgacgagtc tatttacgga atggtatatg ggcaattata
    21181 ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg atcgagagtt
    21241 tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc gtagtgggga
    21301 tgccagattt acaccccgga cgcggctacc cgattggcgc agcgttcttc tccgcaggcc
    21361 gtttttaccc agcactggtc ggcaatgata tcggctgtgg catggcgcta tggcaaacag
    21421 atattctcgc tcgcaaatac aacgccgata agtttgaaaa gcgattatca gctttggatg
    21481 acgttgctga agaaagctgg ctggaggaaa acctgccgtc agcatttgca cagcatccgt
    21541 ggcgcagctc gcttggctct atcggtggtg gcaatcattt cgctgaactg caacaagttg
    21601 atcaaatcat caacgctgaa ctgtttgcac tggcaggtct ggatgcgcag catctgcaac
    21661 tgctggttca tagcggctcg agggggctag ggcagtctat tttacagcgg catattgcct
    21721 cgttttcgca tcatggtttg cctgaaggca gtgacgacgc gctggcgttt gcgcgtttta
    21781 atcgccacct gatcgctttg cgcataatgc aacaggttaa ggccaccgga agtccggttc
    21841 tggatgtggc gcataacttt gttagcgcgt gtcgaatcgg tgatcaacaa ggggtcttac
    21901 atcgtaaagg tgccacgcct gatgactgtg gtctggtggt tattcccggt tcacgcggtg
    21961 attactccag gctggttcag cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
    22021 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
    22081 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
    22141 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtcagggca
    22201 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
    22261 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
    22321 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
    22381 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
    22441 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
    22501 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
    22561 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
    22621 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
    22681 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
    22741 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
    22801 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
    22861 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
    22921 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
    22981 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
    23041 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
    23101 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
    23161 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
    23221 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
    23281 tagtctttac cgctgtcgat tagtgaacgg atcaggcagt gaatttctac gttattgtca
    23341 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
    23401 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
    23461 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
    23521 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
    23581 tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
    23641 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
    23701 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
    23761 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
    23821 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
    23881 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
    23941 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
    24001 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
    24061 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
    24121 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
    24181 tgtaagacga ccggtttacg attttccata cctgcggtag caggttgacg aatcaggata
    24241 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
    24301 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
    24361 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
    24421 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
    24481 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    24541 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    24601 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    24661 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    24721 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    24781 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    24841 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    24901 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
    24961 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    25021 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
    25081 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
    25141 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
    25201 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    25261 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    25321 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    25381 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    25441 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
    25501 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
    25561 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
    25621 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
    25681 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
    25741 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca
    25801 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
    25861 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
    25921 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
    25981 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
    26041 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
    26101 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
    26161 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
    26221 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
    26281 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
    26341 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
    26401 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
    26461 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
    26521 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
    26581 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
    26641 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
    26701 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
    26761 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
    26821 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcgaat
    26881 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
    26941 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
    27001 atattcagct tattatcgct atccagttgg ttgattttat aatcaacaaa cgctgacata
    27061 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
    27121 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
    27181 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
    27241 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
    27301 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
    27361 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
    27421 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
    27481 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
    27541 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
    27601 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
    27661 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
    27721 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggca
//
LOCUS       DAJTYL010000050        27315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYL010000050 DAJTYL010000000
VERSION     DAJTYL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.050"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..318)
                     /locus_tag="OO572_002035"
     CDS             complement(<1..318)
                     /locus_tag="OO572_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6661041.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMG"
     gene            complement(305..1327)
                     /locus_tag="OO572_002036"
     CDS             complement(305..1327)
                     /locus_tag="OO572_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6661042.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1341..2843)
                     /locus_tag="OO572_002037"
     CDS             complement(1341..2843)
                     /locus_tag="OO572_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6661043.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(2983..3939)
                     /gene="ytfQ"
                     /locus_tag="OO572_002038"
                     /pseudo
     CDS             complement(2983..3939)
                     /gene="ytfQ"
                     /locus_tag="OO572_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4249..4779
                     /gene="ppa"
                     /locus_tag="OO572_002039"
     CDS             4249..4779
                     /gene="ppa"
                     /locus_tag="OO572_002039"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6661044.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5159..5500)
                     /locus_tag="OO572_002040"
     CDS             complement(5159..5500)
                     /locus_tag="OO572_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6661045.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5503..9282)
                     /gene="tamB"
                     /locus_tag="OO572_002041"
     CDS             complement(5503..9282)
                     /gene="tamB"
                     /locus_tag="OO572_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6661046.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9279..11012)
                     /gene="tamA"
                     /locus_tag="OO572_002042"
     CDS             complement(9279..11012)
                     /gene="tamA"
                     /locus_tag="OO572_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6661047.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11218..11856
                     /gene="msrA"
                     /locus_tag="OO572_002043"
     CDS             11218..11856
                     /gene="msrA"
                     /locus_tag="OO572_002043"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6661048.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12179..13522
                     /locus_tag="OO572_002044"
     CDS             12179..13522
                     /locus_tag="OO572_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6661049.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13584..13790)
                     /locus_tag="OO572_002045"
     CDS             complement(13584..13790)
                     /locus_tag="OO572_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6661050.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14115..14669
                     /locus_tag="OO572_002046"
     CDS             14115..14669
                     /locus_tag="OO572_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6661051.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14732..15670)
                     /locus_tag="OO572_002047"
     CDS             complement(14732..15670)
                     /locus_tag="OO572_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661052.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(15882..16622)
                     /gene="cysQ"
                     /locus_tag="OO572_002048"
     CDS             complement(15882..16622)
                     /gene="cysQ"
                     /locus_tag="OO572_002048"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6661053.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            16812..18755
                     /gene="cpdB"
                     /locus_tag="OO572_002049"
     CDS             16812..18755
                     /gene="cpdB"
                     /locus_tag="OO572_002049"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6661054.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(18881..19261)
                     /locus_tag="OO572_002050"
     CDS             complement(18881..19261)
                     /locus_tag="OO572_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6661055.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19350..20210
                     /gene="qorB"
                     /locus_tag="OO572_002051"
     CDS             19350..20210
                     /gene="qorB"
                     /locus_tag="OO572_002051"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6661056.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20319..21284
                     /locus_tag="OO572_002052"
     CDS             20319..21284
                     /locus_tag="OO572_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6661057.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21392..22054
                     /gene="ytfE"
                     /locus_tag="OO572_002053"
     CDS             21392..22054
                     /gene="ytfE"
                     /locus_tag="OO572_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6661058.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22099..23511)
                     /gene="cycA"
                     /locus_tag="OO572_002054"
                     /gene_synonym="dagA"
     CDS             complement(22099..23511)
                     /gene="cycA"
                     /locus_tag="OO572_002054"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6661059.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(23819..24439)
                     /gene="fklB"
                     /locus_tag="OO572_002055"
     CDS             complement(23819..24439)
                     /gene="fklB"
                     /locus_tag="OO572_002055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6661060.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24658..25203
                     /locus_tag="OO572_002056"
     CDS             24658..25203
                     /locus_tag="OO572_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6661061.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25187..25834)
                     /locus_tag="OO572_002057"
     CDS             complement(25187..25834)
                     /locus_tag="OO572_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6661062.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26046..26837
                     /locus_tag="OO572_002058"
     CDS             26046..26837
                     /locus_tag="OO572_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6661063.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26847..>27315
                     /locus_tag="OO572_002059"
     CDS             26847..>27315
                     /locus_tag="OO572_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6661064.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADR"
BASE COUNT         6696 a         7263 c         6727 g         6629 t
ORIGIN      
        1 ccccataaat gcgccgaagg cacagcccat caccagcact agcggaaacg ccagcagcgg
       61 cgagaggccg aaatcgccaa ccacttttgc cagaaacacg ccagtaaagg cgatcaccga
      121 accgacggag agatcgatac cgccagagag gatcacaaag gtcatgccaa cggcgattat
      181 cccaagaaag gcgttatcgg tcaggatatt gcagatcacc cttgtggaag caaaaccggg
      241 aaactgggtc aggcagtaga gatagcccag cacaaagacg ccgatggtga tcatcagcgg
      301 caagttacgt tttatcatgg ctacgtactc ctttaatcag actgataaag cgttgcgact
      361 gaacaatcag cacacaaagc accaccaccg ctttcaccac ctggttcatc tccggcggaa
      421 agcccgaaag caaaattccg gtgttcatcc cctgaataat cagcgccccc actaccgaga
      481 gcagcaggtt aaagcgcccg cccatcagcg atccgccgcc aatcaccacc gcgagaatgg
      541 cgtccagctc cagccataat ccggcgttgt tggcatcggc accgcgaata tccgccgcca
      601 cgataatgcc cgcaatcgcc gcacatagcc cgctcaacac gtaggtgagc atgacgatga
      661 ttcgcgtgtt taccccggca ttttttgccg cccgaatgtt gataccaacg acttcgataa
      721 acatccccag cgccgttttg cgggtcaaca gccagaacag gagaagcgtc agcaccgcga
      781 taatgaccgg cgttggcagg aacaacagcg atccgctgcc aaaccatgag agatccggcg
      841 agttaaacgt gacgatctgc ccggcggtga tcagttgcgc cacgccgcgc ccggcgacca
      901 tcaggatcag ggtggcgaca aacggctgaa ttttgaggat cgctaccagt atgccgttcc
      961 acaatcccgc caggatgccg gtgcccaggg cgcttaacaa aacaatcggc aggctgaatc
     1021 ccgcgaccgt catcgcagcc gttgtggctc cggcgatagc catcaccgcc cccacagaga
     1081 gatcgatacc accggtggcg atcaccagcg taataccaat cgccagtaac gcaacgggag
     1141 ctgcacggtt aagaatgtcg atagggctac cgaacaaacg cccatcctga agcaccacct
     1201 gccagaaatg cggggctacc aggctatcga ccagcagcac cagcaacagt gccgccagct
     1261 gcggcatccc cgtcggccag tgaaaacgcc tttttggcgg cgtagtgtca gggagagatt
     1321 gaggcatcac actgttctcc ttacgccgca atggcgttca tgatcgccgg aacggaaagc
     1381 tgtgccagcg ggatctccgc cacctgtttg cgatcgcgca tgatgatcac ccggtcggca
     1441 tagcccacca gctcttccag ttcggaggag atcaccagca gcgccagacc gtcagcacac
     1501 agcgtttcaa tcaggcggat gatctcggcg tgggcaccaa catcaatgcc acgggttagc
     1561 tcatcaagga tcagaaattg cggtcgggtc agtagccaac gtgaaagcag cactttttgc
     1621 tgattgccac cggagagaaa ttcaatcggt tgttcagttg aaggtgtgcg aatgccaagc
     1681 tggcggataa agcgttcggc aatctcttgc tgctctttgc gggaaatggg tcgtagccag
     1741 ccgcgctggg cctggagagc gaggatgata ttttcccgca ccgaggcggc ggcgatgatg
     1801 ccatcggttt tcctgtcttc cgggcagaat ccgatgccca gtactgaagc ctgatgtggc
     1861 gatcgcaggg tttgcggttt gcctttgatc aacgccgtgc cgctgtcagc aggtttgata
     1921 ccgaagatca cttcggcggt ttcggtacgt ccggatccca gcaagccagc cagaccgacg
     1981 atctcgccgg ggcgcacttc gagatcaaac ggtgctatcg ttcctttttt gccgtaattt
     2041 ttgaacgcgg caacgggttt gtcgctcaac aatgttcgcc cggcacgctg tagcgcgtgg
     2101 gtatccagct cgcgccccag catcattttt accagttcga tctgcggtag ctcgcgcgtt
     2161 tcccgacagc ctacgaaact gccgttgcgt aagacggtga tccgatcgct gacctgatag
     2221 acctgatcga gaaagtgagt gacaaagatc aggctgacgc cgcgatcgcg caactgacgc
     2281 atcaggccaa ataatagctc catttcctgg gtgtcgaggc tggcggtggg ttcatcgagg
     2341 atcagcactt tggcggagag atcgatagcc cggcaaatgg cgacgatttg ctgcatcgcc
     2401 accgaaaaac ggttaagcgg ttcgcgcacg tcgagggaga aaccgtaaga tgccatcagt
     2461 tcggtggcgc gcttttccat ctctttacgg cgtagaaggc cgaaacgttt gggttcgcga
     2521 cctatgaata gattatcagc aaccgacata ttgggtagca ggttgacttc ctgatagacg
     2581 gtgccgatac cgagttgttg tgcatgggcg gtatttttcg gtgagatagt ctggccttcc
     2641 agccagatgg tgccgcgatc ggcgtggtat acaccagtta atgctttgat tagcgttgat
     2701 tttcccgccc cgttttcacc aagcagcgcc atgatttcgc cacggcgcag gctgaaatca
     2761 acgttgtcta acgctttgac gccggggaaa aatttactta atccttcggt gcggaggatc
     2821 tcctggtgtt ggtcggtcgt catggtttcc cccggtgtaa agcgtgctcc cagtaggcat
     2881 gataagacgc ggtaagcgtc gcatcaggct tcagtgcacc atcgccggat gcggcgcgag
     2941 cgccttatcc ggcctacgaa tcggcatcag gcatagcaac cctcaatacc ccatattttt
     3001 cttcttctct aactcttctt ttgcggtatc aggcaggtaa agggtggatt tggttaacgt
     3061 cagcttttca ggcatggtgc cgtctttttt gtatttctcc agcgcatcga aagcgggtcc
     3121 cgccatgttc ggcgtcagtt caacactggc gttcgcttcg ccatccatca tcgctttgta
     3181 gatgtccggt acaccgtcaa tggaacccgt gaggatatct ttgcccggtt tcaggcccgc
     3241 ttctttaatt gcctgaattg caccaatcac catgtcgtcg ttatgggcgt aaaccatgca
     3301 gatgtttttg ccgttgtttt ccgctttgat aaagctctcc atgacttctt tgcctttact
     3361 gcgggtgaag tcacctgact gcgagcggat aattttgata tttggcgcat tcttaatggc
     3421 ttcggcaaag cctttcttac ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc
     3481 caccacgttg catggtttgc cattcacttc ttttaccagc cagtcaccga tcaacttacc
     3541 ttcgaggatg ttgtcggcag taacggtggt catatagaga gatttgtctt tcacatcgat
     3601 ggaacggtcg agcaagaaga ccgggatttc ggcatctttc gcctctttta ataccggttc
     3661 ccaacccgtc gcaaccactg gagcgataaa gatcgcatcc accccttatg caacgaagga
     3721 acgtaccgct ttaatctggt tttcctgctt ttgctgacca tcggcaattt tcaacgtaat
     3781 tccgcgtttt tcggcctcac ttttcgccac attggtttct gcggcgcgcc agccggattc
     3841 cgatccgacc tgcgaaaatc ctacggttaa tggagcggcc aacgccatag acgacatggc
     3901 tgccgagact gcagagacta caagtaagcg tttccacata gggttgtcct cgtcggggta
     3961 attattattg gtgaaaagat gttcgcgaaa aaactataga caattcgtta tgtaacggat
     4021 tgcgttacat cacacttcaa aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt
     4081 tcatctcact atgaaaaatg cggctacggt tatggatttt cctgctctgt ataccgtctt
     4141 aaaactggcg aaaaaggaaa atgaagacga aaacaagcaa agacattcgg cgcgagttgg
     4201 ctataatact tggcacttgt ttgccacata tttttaaagg aaacagacat gagcttactc
     4261 aacgtccctg cgggtaaaga tctgccggaa gacatctacg ttgttattga gatcccggct
     4321 aacgcagatc cgatcaaata cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc
     4381 ttcatgtcca ccgcgatgtt ctatccgtgc aactacggtt acatcaacca caccctgtct
     4441 ctggacggtg acccggttga cgtactggtc ccgactccgt acccgctgca gcctggttct
     4501 gtgatccgtt gccgtccggt tgccgttctg aaaatgaccg acgaagccgg tgaagatgcg
     4561 aaactggttg cggttccgca cagcaagctg agcaaagaat acgatcacat taaagacgta
     4621 aacgatctgc ctgaactgct gaaagcgcaa atcgctcact tcttcgagca ctacaaagac
     4681 ctcgaaaaag gcaagtgggt gaaagttgaa ggttgggaaa acgcagaagc cgctaaagct
     4741 gaaatcgttg cttccttcga gcgcgcaaag aataaataag ttcttctagc gcaataaccc
     4801 tgaacgccgg gcttcggtta gtaagggttt ttttatgccc gcgataaata aactctctat
     4861 tccaccatca ttattctcag cggttgcaag gcttgaacgg taagaacaag caaacccgac
     4921 caccattttg ctgttcatag ccacttgctg gaagttagcc gacctcactc atactcaccg
     4981 ttcaaagcgt gcccccgttt tacccctgca catttgtgaa cgccagcaga gttttttatg
     5041 cgattgcaga tatggaaagc ggctcggaga gaaaagcaaa agatggaaaa gttacaaaaa
     5101 aaaatcgcga gatacatcgc ataaaacaac gccacccgaa ggaggcgtgc ttttattatt
     5161 acttatccct gtctaaccag tcgccgcttt caattagctt taatccatcg acgggtcgtt
     5221 gataaacgta catccatgca ctcccatacg gcgtctgaat caactggcgc gcgtattcac
     5281 cgcccctggt gcgcaaggca tcaagttcgg ccagcgtggc gttgtcaata cgataaactt
     5341 caccgtgtac cgttccgttc cccggaactg cgcctggata gtggcccagg ctatacaact
     5401 ggtagttatc gatactgaaa tcgcccagta actgggcatt ggtcatccag tgactgttgc
     5461 cttgtttgtg gcgtaaactg ccgtagacaa atattcgcat tgctaaaact cgaactgata
     5521 gagcaaatcc agtgcctggt ctacaccaga cacggcttcc agatatagct taggcatcag
     5581 gcgataacgt aacgtgagtg ttgctataga gtcaaatata cccacgccgt atttcacttg
     5641 cagacctggc aatacatagc cgctgaccac tacctgggag gagtcgccta ctcccagggt
     5701 gtcgagcgct aaattgctta cgccaaacgt ctcgccgatt ttacccacaa tctggccact
     5761 ttgcgcaacc cccagaccaa tcagcatcga ggtcattgcc gcactgtcgc tctgatcgct
     5821 ctccagccct tgtccacgta gcaaataaga caatgcagct tgttgcgaca tcgccgggtc
     5881 agagaagatc tccgctttcg gttcgtccgc cagaccagtg acgcgaactc cggcgattac
     5941 atcgtcttct gtagcatccg ggttacgaat agcttcaata ttaagatacg gttgatccgg
     6001 cggaccagag aacagtaact cacccttacg cacaatcaga tcctgaccat aggcatggaa
     6061 gcgcccttca gggatgttga tctgcccgtt caggcccagc ccttgtttgt cctgtacgac
     6121 gttaagatcg cccgtcagcc gcgctttcag gccaaaggcg tcaatgcgca cattgttgcc
     6181 aacgtggaca atcaggttac tgttaatcgg aatcgacgcc gttttcggct cttccggttg
     6241 caggttatcg ttaagcatca ccacatcgct ggagacgcct actgcgcttt ccggcagatc
     6301 gtgcaccacg atgcgcgccc acgggacatc cacgcgacca tcgagggtaa acaggtttgg
     6361 tgtagcctcg aatacaacat ctggcgatac atccattcgt accatcggcg gcacggtgat
     6421 ccgcacttta ctgcctttcg ccgttactcg tgcccgccag ttttcaattt ggctccagtc
     6481 ggcatcaccg ttcaggtaga tttcaccctg ctgggtccgt actgtaccgg caagcgtcga
     6541 gcgcataccg ttaaagttga ccgcaagctg gctcggctgc atatcaaacg gcataaagtt
     6601 gccgtcgata tccacacccg taacctgaag ctgaccaaac aactgcgggc tttgcacatc
     6661 accacccaga cgcaagttgg cactcaccat ccccgctgct ttttccccac gggtaaagat
     6721 ggggtttatc atcgcaaggt tgaagttacg gatattgacg ttgccaccaa gattacggcg
     6781 gccttgcgga tcggtcacct gcacctgtcc atcaaactgg ccgttattgg tcaggcggat
     6841 ggtccagccc aattcggcac ggttgttacg caattccgcc gtcagattca gtgtctgaaa
     6901 cgccaccggc agcgccgcat cgttgacggt ttgcgttacc tgcacgttac gccccgaaag
     6961 ggtgatactg ccctgcggca gcccctcttt cgtggtgtcc caggcaacat ccgctttacc
     7021 cgtgaagata ccgctggcct gagtggtttc tggcataaac ggtttcagca tggcgaggtc
     7081 gaagcggttg agattcacca ccgcacgccc ttcggccccc gcatcgatag tttgcggcac
     7141 gcacagttcc gcattcgggt taagccagca gtgtggcccg atgctgattt tttgctcctt
     7201 attgcggtaa tccagcgcaa tatcgcgggt cagcgaccac gggccgaccg gcgtctggaa
     7261 gcgggtattg ctaagagttc ccttccagcg ttcttctttg cgatcaaaac ttcctgccag
     7321 attaagctgc ccggagacag gctcgccctg aatccgcaac tgtagctcgt gctgcttttc
     7381 gctgcctttg gcattcaggg tgacgaggtt gatatttaca tccggctgcg aaatttgctc
     7441 aacgcgtacg tcgagtttcc cggcgatctg atcggtggat ttaatgtcgc cttccacgcg
     7501 aacctgcgcc acggaaagtt cctgccagcg caggccgcgc gcggtgatat ctgccagtag
     7561 ttgtggcgct tccaccgtgc cgcgtacttt caccagccct ttcgctgtac cgccaagccc
     7621 cggcagcgcg ttatccagcc ccggcgcgtt gatggtggca tcaagattga gatcttttac
     7681 ccccagctcg cctttcactt cggcactgtt tggcccgagt tccagatgaa gcccagggat
     7741 catccactgc atataactgt tgcctttcag cgtgccgtca acgttcactt tgttctgttt
     7801 gacgttaccg gtcagcttca gttctggcac ctccatctgc caggtgccgc cgtacaggct
     7861 accgcgggtt ttaatcaagc cattgagttt cgacggccac tccgggaact ctttggcggt
     7921 gttaatgcca ttaagcgtta gctcaccgcg ccaactaatg gcctgctgcc agtcgagcaa
     7981 cgccttgagt tcagttttcc cttccagcgc cgcgacggtg agtttgtcga gattgacctg
     8041 ctgttcatta cctttggcat caagggtaat ggtggctggc gggatctcct gtcccttcac
     8101 tgccgtacgc atagagagcg tgtaatcggt cattttgccg gtaagtttca gtttcagatc
     8161 atccgcctga tactgcttct caccagtgaa cggccagtaa agctgtttgc tgttcacttc
     8221 cacgttgagt ggcaatccgg cttccgccag tcgcgtctgg gcgcgtaaat ccatatccac
     8281 cggaccggaa aggttaacgc ctatctccag ctgttcgcgc agcgcgccgc ccactttcag
     8341 cttcaccttt tcacctttca acggctccac gttcagtgtg ctgttgaggg tgatatccac
     8401 cggccagttg tctgacagct gcgccgtgcc gctggcgttg acgatcccct ggttggaatc
     8461 gatatccagg gcgtccagtt tagtattgcc gtcaatgctg ctcactttca gcagcatggt
     8521 gcgcacggtg atgtccgtgt cgcccgtcac gcgcagctgc tcgcccttaa actcttcaat
     8581 gttcaggtta agcggcaaat gcacgtcggt catttccggc aataccgggc gagaaaagag
     8641 atctttcagc gtttcgccga gcggcttttc ctccggctgc ggattttcaa ttttcggttc
     8701 gaccacttct tcctgcgcca cttccaccac tttcggcaga gcaatcagca ggcctttcag
     8761 cgacgtcggt ttcagggtca gggttttctc ctgccagttc aggccggagg tgaagtccat
     8821 caccgacacc gtggtgtcat caatcttgat gttgacgttg tccagcgcca cccgtgtcag
     8881 ggtgatggga tacggcgtgg agagatccag cggaccgcta tcttcctctt cttcaacctg
     8941 ttcagaagga ggcatttttt tactgtcgat gttgacctga atgtctttca gcgccaggtc
     9001 attaatacaa acgctactgt tccacaggca ctcaagaccg acccccagat gcagattacc
     9061 cgcttttacc gccacgccta gctgctcata acgaacgtca gacaaggtga gatcgcgcca
     9121 gccgccggtg accttgccaa tatccagtcc tggcacccag cgatccgccg ctttaaatac
     9181 aagatgcagg ccgctggtgg tgcccaccag aaacgccacc gatcccagca acagtaagat
     9241 aacgataacc acgccgaggc tgattttttt ccataaactc ataattctgg ccccagaccg
     9301 atgtaaaact gtaacccgtg ttcgtcttta tccgcgaccg gtacggcaaa atcgagtttg
     9361 attggcccga ccggcgattc ccagcgtacg ccgaccccgg taccggtttt aaagtcgctg
     9421 cggcgaatat cgcttaccgc ttcgccacta tcgacaaaca ccgcgcccca ccattttccg
     9481 gtcacgttgt actggtattc cagcgatccg gttatcaact tcgaggcccc tttcaggtca
     9541 ccgttggcgt atttcggggc gatagatttg tatttgtagc cgcgaatact gcgatcgccc
     9601 ccgacgaaga aacgcagatc cggcggtact ttgtcgaaat caccggtttc aatccagccc
     9661 agcgtgccgc gtgtcacaaa gcgatggcga tcgtacagtg tgcggatcca gacgtgctgc
     9721 gcctggaaaa cggagaaatc gacatctgaa ccccaggccg tgttggagta gtcgatagag
     9781 tagcgttgcg agtctcccca ggttggcatc aggccaccac gagaacgcgt gcggctaatc
     9841 atcaccccag gataaaacag catcgtggtg ttggtaattt caccctgggt aaagtggtcg
     9901 aggctccagc gcaggttaat ggcacgctgc cagccgctgg agagatccca gtagcgagaa
     9961 gccaccagcg tagtggagtc agattcggta tcgttcaggt cagtgcgctt aaaaccgccc
    10021 tgcaccaaat aatattgttc cagtggattc ttcagcagcg gcattttata gctgaagtcg
    10081 agggtctgtt ccggcgcgga aatactggta ctggtggtca aactgtgacc gtatgagttc
    10141 atccacggct ttttccacgt cgctttcacg cgcggtccca cgtccgtaga gtaaccgacc
    10201 ccggtttcga tggtgttttc tgttcgcggc gaaaccacgc ccgtcaatgg taatactttc
    10261 gtttcgcgcg ctttatcaaa ttgtggagcc accaccaccg agttaaacca gccggtagca
    10321 gaaagtcggc ggttcagttc cgccagatct ttcgattcat actcatcgcc ctctttaaac
    10381 ggcaccagat tttgcaggta ttcatcgcgg atttgtgatc cttcaaaggt cacatgccca
    10441 aagcggtaac gttcgccact gttataatca atatcccaga aggctttatg caggccgagc
    10501 gcaatgccca gctgcgcttt ggtaaattcg ctatcgaaat aacctttacg caacgcaata
    10561 ctggttaagg actttttgaa attttcataa tcgccctggt tcagtaccgt accaatagcc
    10621 gggcgagtat cgagcaattt caaatagtct ttatcggtcc gcgcgccgcc gcgcaatacc
    10681 acatcggtgc cgccaattaa caccggcaca cctggcgtga ctttggcgat caatacctgc
    10741 cgccctttct ttggcggtgg acggagatca aattcaatgg tcggctggta ataacccagc
    10801 gctttcagac cttcacggat ggcatcatcg acgcgtgcgc gaaagcgacg gtctggcgtc
    10861 acttcatcac tttcaatcgt agaaagctgc gcacgaacgt tcttttccag ctgtcccgat
    10921 aacccctcga cctgtagacg gacgttcgcg gcgacggcag atccgcttaa gcagagtaag
    10981 cttacacagc ataactgtcg gatatagcgc acattttctc ctgaatatcc ttttttcctg
    11041 cccctggaac gccgttaaac ggcttaacaa aaatccagta atatggatta aaaagcagac
    11101 taaaccccaa atatttctta tgttttactt tagacctatt cacggtggtt attgtgtgca
    11161 aatacgcctc ttgttacaac cttaacccca atgaccgatt ttcgggagag cgacaccatg
    11221 agtttatttg ataaaaagca tctggtttcc cccgccgatg ccctgcctgg acgtaacacc
    11281 ccgatgcccg tagccacgct gcatgcggtc aacggtcact caatgaccaa tgtacctgac
    11341 ggaatggaga ttgccatttt tgcgatgggt tgtttctggg gtgtggagcg tctgttctgg
    11401 cagttacccg gcgtttacag caccgccgca ggctataccg gcggctatac gccaaatccg
    11461 acttatcggg aagtgtgctc cggtgatacg ggtcatgccg aagcggtacg cattgtttac
    11521 gatccttccg tcatcagcta tgagcagttg ctacaggtat tttgggagaa tcacgatccc
    11581 gcccagggaa tgcgtcaggg caatgaccac ggcacgcagt atcgttcagc gatttatccg
    11641 ctgaccccag aacaggatgc cgcagctcgc gccagtctgg aacgttttca ggcggcgatg
    11701 cttgccgccg atgacgatcg tcacatcacc acggaaatcg ctaacgccac accgttttat
    11761 tatgccgaag atgaccacca gcaatatctg cataaaaacc catatggtta ttgtggaatt
    11821 ggcggaattg gcgtctgtct gccgccggaa gcatagcgtt acgggtacaa atgtagtttg
    11881 ttgataaagt gctctttgtc tatgcctgat gcggcttaaa cgacttacca ggcctacaaa
    11941 atcgcccaaa ttcaatatat tgcaaggact gcgtaggcct gatcggcata gcgcatcagg
    12001 cagtattgca tttatcagca gtctgaattt taaccctctg gcgactttac agtaccttac
    12061 gctatactag ccactgaaaa tgccggttca ctttcttcga atcggctttc aatgtgtatt
    12121 tcacacaaat taatcaactt tcccttccga gggtctggcc tgaaaggtcg gataagatat
    12181 gttaaacagt attttagtca tactctgctt gatcgctgta agtgcgttct tctcgatgtc
    12241 cgagatctcg cttgccgcct cacgcaaaat caaacttaaa ctgctggctg atgaaggcaa
    12301 tataaatgcc caacgcgttc tgaatatgca ggaaaatccc ggcatgttct ttaccgtggt
    12361 ccaaatcggt ctgaacgcag tggcgattct cggcggtatc gtcggtgatg cggcattttc
    12421 tccagctttt cacagcctgt tctcccgcta tatgtcggca gagctctctg agcaactgag
    12481 ctttattctc tctttctcgt tagtgactgg catgtttatc ctgtttgcgg atttaacccc
    12541 gaaacgcatc ggtatgattg cgccagaagc ggtggctttg cgtatcatca acccgatgcg
    12601 cttctgcctg tacgtttgca ccccgctggt gtggttcttc aacggcctgg cgaacatgat
    12661 cttccgtatt ttcaaactgc caatggtacg taaagatgac atcacttctg atgacatcta
    12721 cgcggtagtg gaagccggtg cgctggcggg cgtgttacgt aaacaggaac acgagctgat
    12781 tgaaaacgtc tttgagctgg aatcccgtac cgttccgtct tcaatgacac cgcgtgaaaa
    12841 cgtgatttgg tttgatctcc acgaagatga gcaaagcctg aagaataagg tggcggaaca
    12901 tccgcactct aagttcctcg tctgtaatga agatattgac cacatcatcg gttatgtcga
    12961 ttctaaagac ctgctgaacc gcgtgctggc taaccaaagc ctggcactga acagcggcgt
    13021 acaaattcgc aacacgctga ttgtgccgga tacgttaacc ctttcagagg cgttggaaag
    13081 ttttaaaacc gcaggtgaag acttcgcggt gatcatgaac gagtacgcgc tggtggtggg
    13141 gatcatcacc ctcaacgacg tgatgaccac gctgatgggc gatctggtcg gtcaggggct
    13201 ggaagagcag attgtcgccc gtgatgagaa ctcatggctg attgacggcg gcaccccaat
    13261 tgacgacgtc atgcgcgtgc tggatattga cgagttcccg cagtcgggca actacgaaac
    13321 catcggcggc tttatgatgt ttatgctgcg taagatcccg aaacgcaccg attcggtgaa
    13381 attcgccggc tacaaatttg aagtggtgga tatcgataac taccgcatcg accagctgct
    13441 ggtgacccgg atcgacagca aggccaccgc cctttcgcca aaactgcctg acgctaaaga
    13501 taaagaagaa agcgtcgcgt aacccccaga aacatcaacg gctcctgaat caggagccgt
    13561 cttattactg catagcactt tggttaagcc atctctgttt gcagacgcat aacctgacgg
    13621 ttaacttcgg acatcactga caaatgcagc ttatccttca cttttgggat aagaatctta
    13681 cccttatcaa attcaaaagc gccaatgtcc ttgatgtata accgtccacg gaacaggatc
    13741 tttacgtact tcgccacctg aagtgggttg taccgttgga aaattttcat tgttatctcc
    13801 tgctgagtat tacgcctgtg cggtgccaca atcggcccaa ctattatgag gcgcaaattt
    13861 taatgcttag tgactataga ctatccgggc aatgtttcca ccgcgtataa ctttttttac
    13921 ctaaaggtta caattattca gaattatctt tttaccgaag cgcggtcttc agtataagca
    13981 ttcatttttc atatgatttg tgcgcttgac cgcaaactgg catcacactt gcgggaaatt
    14041 cgataaatag cacatatgat taaaactcag acccaagtgg tcggatcacc tgcatatcat
    14101 aagaaggaaa caccatgacc ctacgcaaga ttctggcact cacctgcctg ctgttgccga
    14161 tgatggcttc cgcacatcag ttcgaaatcg gtcagcgagt gccgccgatt ggcattaccg
    14221 atcggggcga gttggtgctt gataaagatc agtttagcta caaaacctgg aacagcgcgc
    14281 agttagtggg aaaagtgcga gtactgcaac atattgctgg tcgcacctct gcaaaagaga
    14341 aaaacgcgac gctgattgaa gcgattaaat cagcgaagtt accgcacgat cgttaccaga
    14401 ccaccaccat tgttaacacc gacgacgcga ttccgggttc cggcatgttt gtgcgcagca
    14461 gtctggagag taataaaaag ctttatccct ggtcgcagtt tattgtcgat agcaatggcg
    14521 tcgcgcgcgg tgcctggcag ctggatgaag aaagttccgc tgtagtggtg ctggataaag
    14581 acggtcgcgt gcaatgggcc aaagacgggg cgctcacacc ggaagaggtg cagcaagtga
    14641 tggacctgct gcaaaagttg ctcaaataat ggcaataaca taaattctgg aaaaataatt
    14701 ttcccctatc gttatctatg ggatagggga attaagtctc tgagttctta caccgaacta
    14761 atactggtaa cgctagttgc tcttttttaa acgcctcaat ggtcgattta tatttctcgc
    14821 aaatcttttc tttggtaata ttactatctg cggccagatt cgcacttact atcgcatcca
    14881 cttgttcggc taattcgact tgttggcaac ctgcattaac cagaaaatta taaataagag
    14941 agtaatcttc atcctccaaa acaacagagg aaccattacg aaaacgttca tcccctccat
    15001 agattttaag cgcaaagccc aaagattcta tgactatggc aaaattatca tcaattattt
    15061 tttcgtaaat ataccatcca tcgcattctt taattccacc gcctggtatt tggagttcaa
    15121 atttaccccg ttttaatttg cttactacat cctccaataa ggcataaccg aaagatacgt
    15181 caccaaacgc ctggcgaata taacctttaa tcctttttgg tatgtcagac tgttttttct
    15241 gcaaaatatc acaggcatac aatcttctgt tttgttctaa cggaaactcg cggtttttga
    15301 aatccatgaa tgagttatac tcatttattg ttatgggaat ttcttcgtat tcaaacccac
    15361 tgcctcgcaa tgttattgta tattcagcac cgttatcttt aaggctgaaa ctgataagaa
    15421 aattatccat ggcattaaca gtttcatttt ttgatgaaag cacttgataa ctatttttat
    15481 caatctgtgt tgcgcaacga aaggactccc gcaaagttaa cagactatca taattcgaaa
    15541 cccaatcttt tttcttaaat gcatgctgaa tattattaaa aaaatcgttt gaatgtgttt
    15601 ttgtatgcga tgactttaca aataactcca ccgcaccaat aaaattattg tgtctgatcc
    15661 tgggtaacat atggctacct tattttattt ggttatttta tgtcgccatt aaatatctca
    15721 ttatagaagt aaactatcaa aaaacgctca ttattaaaat gtaacttact agcaattgaa
    15781 tttttgcact ttaaatacaa tctcttttta tttataactt tttaggttgg gataaatcag
    15841 gatggcagaa tcaggaaata cactgtttct gccatctgaa tttagtaaat ggacactctg
    15901 aaccccggat tcaggaacga ctcgcgcgga gtgtaatcca gcggtttacc ctgccagtcg
    15961 tgaacgtgcg ctccggcagc tgcagccaca gcatgtccgg cggcggtgtc ccaaatattc
    16021 gttggtccga agcgcgggta cagctgcgcc tgtccttccg ccaccaggca gaatttcagc
    16081 gaagagccga tggacgtggt ctgatgttcg ccaagctgtt gcagatactc tttcagctcc
    16141 gcatctgcat gggaacggct gataaccacc agcggcgggc gcgcatcgcg gacctgaatc
    16201 agcttgcgca caccgcactc ttctttccag gccttgcctt ctgccgcgct gtacattacg
    16261 ttcattaccg gcgcatacac cacacctaaa atcggtttgc catggtcaat gagcgcaatg
    16321 ttaacggtga attcgccatt acgtttaata aactctttag taccatccag cgggtctacc
    16381 agccagtaac gctgccagtg ctgacggact tcccaaccgg gaggatcttc ttcagaaagg
    16441 accggaatat ccggtgccag cgtacgtaaa ccgtccatga taacggtgtg agcggcaata
    16501 tccgctgccg ttaccggaga attgtccgct ttgctgacga cgtccatcgg tttcgtcccg
    16561 tcgtagacct gcataatggc atcgcctgca ttccgtgcaa gctggcatac ttgatctaac
    16621 atttctccac ctcgtctctg tgagcggtgt taacttattg ttttacttat accctatcgt
    16681 taatgaatgc gccaactgtg atagtgtcat cattttcaaa gcgtaaaatt gtggcattct
    16741 tcactgttct ataagtaagg cgtttattct cccttttctt tcgtattccc gatgataaaa
    16801 ggatgtccct gatgattaag tttagcgcaa cgctcctggc cacgctgatt gccgccagtg
    16861 tgaatgcagc gacggtcgat ctgcgtatca tggaaaccac tgatctgcat agcaacatga
    16921 tggatttcga ttattacaaa gacaccgcca cggaaaaatt cggactggta cgtacggcaa
    16981 gcctgattaa cgatgcccgc aatgaagtga aaaacagcgt actggtcgat aacggcgatt
    17041 tgattcaggg gagtccgctg gccgattaca tgtcggcgaa aggattaaaa gcaggtgatg
    17101 ttcatccggt ttataaggcg ctgaatacgc tggattatac ggtcggtaca ctcggcaatc
    17161 atgaatttaa ctacggtctg gattacctga aaaatgcgct ggcgggagcg aaattccctt
    17221 atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc aatgtttaca ccgtatttaa
    17281 ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca gacgctgaag attggctata
    17341 ttggcgtcgt gccgccgcaa atcatgggct gggataaagc taatttatcc ggaaaagtga
    17401 cggtgaatga tattaccgaa accgtgcgca aatacgtgcc tgaaatgcgc gagaaaggtg
    17461 ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc cgatccgtat aaagtaatgg
    17521 cggaaaactc agtttattac ctcagtgaaa ttccgggcgt taacgccatt atgtttggcc
    17581 atgctcacgc cgttttcccg ggtaaagatt ttgctgatat cgaaggggct gatatcacca
    17641 aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat gtggggcgat catcttgggg
    17701 tggtcgactt acaactcagt aatgacagcg gtaaatggca ggtgacgcag gcgaaagcgg
    17761 aagcacggcc gatttacgac atcgccaata aaaaatccct cgcggcggaa gacagcaagc
    17821 tggtagaaac actcaaagcc gatcacgatg ccacacgcca gttcgtcagc aagccaatcg
    17881 gtaaatctgc cgacaatatg tatagctatc tggcgctggt gcaggacgat ccgaccgtgc
    17941 aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta cattcagggc gatccggatc
    18001 tggcaaaact gccggtgctt tcagctgccg caccgtttaa agttggtggt cgcaaaaatg
    18061 acccggcaag ctatgtggag gtggaaaaag gccagttgac cttccgtaat gccgccgatc
    18121 tttatctcta ccccaatacg ctgattgtag tgaaagccag cggtaaagag gtgaaagagt
    18181 ggctggagtg ctccgccgga cagtttaacc agattgctcc taacagcacg aaaccacagt
    18241 cactcatcaa ctgggatggt ttccgcactt ataactttga tgttattgat ggtgtgaatt
    18301 atcagattga tgttacccaa cccgcccgct atgacggcga gtgccagatg attaatgcca
    18361 atgcagagcg gattaagaac ctgaccttta atggcaagcc gattgatccg aacgctatgt
    18421 tcctggttgc caccaataac tatcgcgctt acggcggcaa atttgccggt acgggcgaca
    18481 gccatatcgc ttttgcttca ccggatgaga accgctcggt gctggcagcg tggattgctg
    18541 atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga taacaactgg cgtttagcac
    18601 cgatagctgc cgataagaaa ctggatatcc gtttcgaaac ttccccgtca gataaagccg
    18661 cagcgtttat taaagagaaa gggcagtatc cgatgaataa agtcgcgacc gatgatatcg
    18721 ggtttgcgat ttatcaggtg gatttgagta agtaaaacac ttctttttcg gcctataaat
    18781 caaccgcatc cggcatttat tggcggatgc gatgctgccg tatcttatcc accctacaag
    18841 ccatgaaccg tagaccggat aagcgcagcg catccggcag ttatgccgca cgttcatccc
    18901 gcaccgccaa cacctcgggc aaattcaact caatccagtc cgccagtgca gcaacctttt
    18961 cgctcacctg ctcgcccagc ggcgtgaggc tatattccac atgcggcggc accaccggat
    19021 acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg taacgactgc gcaagcatct
    19081 tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta
    19141 gcgccaccag aatcaacacc ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg
    19201 ggcactgttc cgcaaagaga ttaccctctt tcagttgttg tgacaggcta acctgactca
    19261 tttcatactt acctttttgc acgtacttac taaaagtaag tttaggtgtt agcgtattta
    19321 aacacaagac aaaacgatgg agacttccca tgatcgctat tactggtgcc actggccaac
    19381 ttggtcacta tattattgaa tccttgatga aaacggttcc tgccagccaa atagtggcta
    19441 tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca aggcattacc gtgcgtcagg
    19501 ctgactacag cgatgaagcc gcactgacat ctgcacttca gggagtggaa aaactactgc
    19561 tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg
    19621 caaaggcggc tggcgtgaaa tttatcgctt ataccagcct gctacatgca gatacctccc
    19681 cgctcggcct cgccgatgag cacatcgaga cggagaaaat gctggctgat tctggcatcg
    19741 tttacaccct gctgcgcaac ggctggtaca gcgaaaacta cctcgccagc gccccggcag
    19801 cactggaaca cggcgtattt atcggtgcgg ctggcgatgg caaaatcgcc tcagcaacgc
    19861 gggcagatta tgcgacagct gcggcacgcg tgattagcga agccggtcac gaaggcaagg
    19921 tttacgaact ggcgggcgat agtgcctgga cgttgacaca gttagcggca gagctgacca
    19981 aacagagcgg caaacaggtt acctatcaaa atctgagcga agccgatttc gccgcggcac
    20041 tgaaaagcgt cggactgccc gacggactgg cggatatgct ggcggattct gacgttggcg
    20101 catcgaaagg tggtctgttt gataacagca aaacgcttag caaattgatt ggctgcccaa
    20161 cgacaacgtt agccgaaagc gtaagccatc tttttaatgt taataactag ttaattaaag
    20221 tggcatcctc ccgcatcctc tctgataatg acgggatgcc gggagcaatc atgtctgctt
    20281 cctgaacgtt tcttctgaca gaccaatgga tgccagtaat gattagcggc gtgctatacg
    20341 ccctgttagc agggttgatg tgggggctta tttttgtcgg gccgttgatc gtgccggaat
    20401 acccggcgat gttgcagtcg atggggcgtt atctggcgtt agggttaatt gcgctaccca
    20461 ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg tcgggactgg cttaccgcct
    20521 tgatgctcac tatgatgggc aacctcattt attacttctg ccttgccagt gccattcaac
    20581 gtactggcgc gcctgtttcc acgatgatta tcggcaccct gccggtggtg attcctgtct
    20641 ttgccaatct gctttatagc cagcgcgacg gcaaactcgc gtggggaaaa ctcgccccgg
    20701 cactgatttg tattggcatc ggcctggcga gtgtgaatat tgctgagtta aaccacggac
    20761 tccccgattt tgactgggca cgttatacct ctggcatcgt gctagcgtta gtttccgtgg
    20821 tctgctgggc atggtatgcc ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac
    20881 atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct gccggtttct ctcatcggct
    20941 atctcgtcgc ctgttactgg ctgaatatac aaacgccgga cttctcctta ccttttggcc
    21001 cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg
    21061 gcgcactctg ctggaacgtc gccagccagc gattaccgac agtgattctc gggccgctga
    21121 ttgttttcga aacgctggca ggtttgctgt acaccttttt actccgccag caaatgccgc
    21181 cgctaatgac gctgagcggt atcgcgctgt tagtgattgg cgtggtcatc gcggtcagag
    21241 caaaaccaga aaaaccttta actgaatctg tctcagaaag ttgacatgct ggcagtgagt
    21301 taaataagcc tctgctacgt aagggttata gcttttgcct taaaggtgta tttaaaatac
    21361 atcttatctt attaagaatg aggtatcagc tatggcttat cgcgaccaac ctttaggtga
    21421 actggcgctc tctatccctc gcgcttcggc tctgtttcgt aaatatgata tggattactg
    21481 ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt
    21541 cattgaagct gaactggcaa agcttgctga acaaccgatt gagaaagact ggcgtagcgc
    21601 cccgctggca gaaatcattg accatatcat cgtgcgctac cacgatcgtc accgcgagca
    21661 gctgccggag ctgatcctgc aagcgactaa agtcgagcgc gttcacgccg acaaaccgag
    21721 cgtgccaaaa gggctgacaa aatacctgac catgctgcat gaagagcttt ccagccacat
    21781 gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa ggcatgggca gccaggcaat
    21841 ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat
    21901 caaacacact accaataacg tcacaccgcc gccagaagcc tgcaccacct ggaaagcgat
    21961 gtataacggc attaatgaac tgattgatga cctgatggat cacatcagtc tggaaaacaa
    22021 tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa agctggatgg cattgcgcca
    22081 tccagcatga taatgcggtt atttccgcag ttcagcagcc cgcttcttac caataaacag
    22141 ccagcccaac cccagcgcga taaaccacag cggggtaacc agcagcgcct ggcgagtgtc
    22201 atcttccagt gtcagcaaca cgaccacgaa cacaaagaac gccatacata cccagcacat
    22261 cagcttgccg agcggcatct tgtagatcga cttttcatgc agatgaggac gctgtttgcg
    22321 gtacaccagg taagagcaaa ggataatcgt ccagacgaac ataaacagaa tcgcggaaac
    22381 ggttgtaatc atcgtgaacg cgccaatcac gctaggattc acatacaaca tcaccacgcc
    22441 gccgagcaga cagatacacg agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag
    22501 tttagcgaac gctttcggcg caacaccttc ctgcgccaga ccaaacagca tacggctggt
    22561 agagaagaca ccgctgttag cggaagacgc cgcagaggtc agcaccacaa agttgatcac
    22621 gctggcagcc gcaggcaaac ctaccaacac aaacagctca acgaacgggc ttttctccgg
    22681 gactaccgaa ctccacggtg tcacggacat aatcacaatc agcgcgaaga cgtagaacat
    22741 aatgatacgg atcggaatgg agttaatcgc gcgtggcagt gatttctccg gatctttggt
    22801 ttccgcagct gttgtaccta ccagctcaat ccccacgaaa gcgaaaaccg ctatctggaa
    22861 tccggcaaag aagccactta aacctttcgg gaaccagccg ccgtcattcc acaaatgcgc
    22921 gaatgacgct tccacaccgg tcggtgactg aaagtgcatc gccaccatga ccaggccgac
    22981 gacaatcagc gacacgatgg cgacgatttt gatcatcgca aaccagaact ccatctcacc
    23041 gaacattttc acggtggcaa ggttgagcgt cagcagcagc actatcaccg ccagcgaggc
    23101 gacccagtcg gagagatcgg ggaaccagaa ctgagcataa gccgtgatcg ccaccacgtc
    23161 tgccataccg gttacaaccc agcagaacca gtaagtccag ccggtgaaat atcccgccca
    23221 cggccccagt aaatcggaag cgaagtcact aaaagatttg tattccagat tcgaaagcag
    23281 caattccccc attgcccgca tcacgaaaaa gagcataaaa ccaatgatca tataaacgaa
    23341 aatgatcgac ggcccggcaa ggctaatcgt tttgccggac cccataaaca acccagtacc
    23401 aatggcacct ccaatggcaa taagctgaac atgtcggttt gtgagattgc gccgtagcga
    23461 ctgttcagcc ggagcctgat catcggcaac gacttttacc tgatctacca tgttttttct
    23521 tcctgtacct gtctgtgttg ttcaggctct acggccttta gtcagtctat gatacgacga
    23581 atctgtatcg ggactcatcg atattaggta acaatacgcg ggatgaatac taagatttag
    23641 atctaatgtt aattttatgt ttaaagtgag tgtcatatca cggtgataac gcgaaacagc
    23701 tcacaaaaat acacgcttac ggggtctttg caacacaaaa tagcgccgtt tggcaaactg
    23761 aagggtttat tgctgaatgc ctgctcccct ctggttcgag gggaacagaa tgcgctgctt
    23821 agaggatttc cagcagttcg acttcaaaca ccagggtgct gaacggaggg atggatgcac
    23881 ctgcgccgcg ctcgccatat gccagttcct gcgggatagt cagttcccat ttagaaccta
    23941 ccggcatcag agtcagtgct tcaatccagc cagggatcac gccattaacc gggaattcag
    24001 cgggttcacc acgagcaacg gagctgtcaa acacggtgcc gtcgatcagt ttaccggtgt
    24061 aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc
    24121 ggaattgcag gccagattcg gtgctattca caccttcttt tttggcgttt tcttccaggt
    24181 atttcacgcc ttcagcagcc atcgcctgga aacgctgacg acgaacggca tcggcgcgtt
    24241 cgtggatttc acgcagcgcg cgatgcacca catcaaccgg cacagccgga tgtttgcctt
    24301 ccagcgcatc ggcaatacct gcaaccagtg cttctggcag cagcccttcc aggccagatt
    24361 cactcagttg ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt
    24421 caaaagttgg ggtggtcatc gtatttcctt tcttagggta taaaagtagc gggcagcata
    24481 tcagccacac ttctttgggt aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa
    24541 agaaacaata gcaatatccc gtatgaatat aataaaggcg ggtttaccac ggacatcata
    24601 gcgttagctt attcataggc tatgattgag gaacaagacg tggagcagga ggaaaccatg
    24661 cccgggcgct ttgaactaaa accaaccctg gagaaagtct ggcacgcgcc ggacaatttt
    24721 cgcttcatgg acccgctgcc gccgatgcat cgtcggggga tcattattgc cgccattgta
    24781 ttggtggtcg gatttctgct tccatctgat gatacgccca acgcgtcggt ggtgacgcgt
    24841 gaagcgcagt tagatattca gtcgcaatca caaccgccga cagaagaaca gccgcaaact
    24901 acccaaacgc aaccattcca gccagatagc ggaattgata atcaatggcg ctcttaccgt
    24961 gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat
    25021 gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt gcaaaatggg
    25081 cagatggtga aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg
    25141 gggaataatc aacaggtcct gtttacccgc cagccggatg gcagttttat tcgtgcgcgg
    25201 tagatgaagg gtcaaccaaa agccagctaa ggattgtctc tttgatgacc ttacgtctgg
    25261 cttccagtgc ctggggggaa accatactga tatgataaac taggccaagc gtatgctggt
    25321 tgatcaaata atatccccca agcgcagcaa tattaatatt cacctgcaat gggtcgatac
    25381 ctggcttaaa aatattgtat ttcttccctt catccaataa ggattccatt aactgcaagt
    25441 gagcatggtt aatttctaag agacgctgtg attttgcata atgcacacct ttactttgat
    25501 tttcactatg cacaattttt aaaaaccaag gattagcaat ataataatcc catgtaaaat
    25561 caataatttt ctcaatggct ttttgtggcg gaagatctga gaaatcaagg gccttttctt
    25621 tttgtcgaat atcgttccag gtatattcca gcacttcgat aaataaattt tctttattac
    25681 caaagtggtg ataaaccgtc tgtttactac accctgctgc ctttacaata ttatctactc
    25741 tggccccttc ataaccatat tcggcaaatt cattaaccgc acatagcagc aatttttcct
    25801 tcaggctctg tacgttgctg gtatcaatat acataacgct tccccacgac ctcttcgtgg
    25861 tgactatcat aacctgattt gatttcagct tcagaaaaaa gggcaaaaac ttgtactttc
    25921 atcaggttat caaatgtcct gtgatcgcca tcgcatatca accaactaga tggtttatct
    25981 ttttatcctc cagtcgcaag ctaatcatca ccaaggaaac aaaattcaaa aaagagagct
    26041 attgtatgaa atccactcgt cctgttgccg tgattaccgg agccactcgc ggtattggaa
    26101 agggctgtgc gcaggagctt gctcgtggcg ggtttaacct gctgattaac gatcgtcccg
    26161 atgccgacag cgtagaaaaa ctgcatatca cacaacaaga atgtttggcg gaaggcgtgg
    26221 aagtgatttg cttccctgct gatgtcggtg atctatctct gcatgaggag atgctcgacg
    26281 cggcgcaaaa ccagtggggg cggctggact gtctgctcaa taacgcgggg atttcagtga
    26341 aaaaacgcgg tgacctcctc gacctcgaac cagacagctt cgatcaaaac attgccatta
    26401 acacccgcgc gccgttcttc cttgctcagg cattcagtaa gcgcttactg gcacagccga
    26461 aaccggaagc ggagctgccg caccgctcga ttattttcgt cagttccatc aacgccatca
    26521 tgctggcgat gaatcgcggc gaatacacca ttgccaaaac cgccgtttct gccgctgctc
    26581 gtctgtttgc tgctcgcctt tgtaatgaac aaattggcgt ttatgaagtg cgccctggtc
    26641 tcatcaaaac tgatatgact attccggcaa cggcttatta cgacgaactg attgccaaag
    26701 gtctggtgcc gtggggacgt tggggttatc cggctgatat tgcttccacc gtccgcgcga
    26761 tggcggaagg taaactgatt tacacctgtg gtcaggcggt cgcaatcgac ggcggcttaa
    26821 gtatgccgcg cttctgaggc tgaatcatga gagatctgtt acaacgcccg gatttattca
    26881 gtatcaatac cgcgacactg ggctataaaa cgccgctacc cgccattatt gatgcctgcg
    26941 ccgcacgcgg tattggcgca attgccccct ggcgcaggga gttgcagggt gaagatctcc
    27001 agcaaatcac ccgccagctt gccgccagta acatgagcgt ttccggtttg tgccgtagca
    27061 cttactacac cgcgccgaca ctggcggaac gcaaactggc gattgatgat aaccgccggg
    27121 cgctggacga cgcagcggtg cttaacgccg cctgttatat gcaggtggtt ggcggtctgc
    27181 caacgggcac taaagatcta tatgaagcgc gagaacaagt gaaacaaggg attcgccagt
    27241 tgctgccaca ttcgaaagat gtcggcgtgc caattgcgct ggagccactg cacccgatga
    27301 cagctgccga tcgct
//
LOCUS       DAJTYL010000051        26843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYL010000051 DAJTYL010000000
VERSION     DAJTYL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.051"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..982
                     /gene="mdtO"
                     /locus_tag="OO572_002060"
     CDS             <1..982
                     /gene="mdtO"
                     /locus_tag="OO572_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6661065.1"
                     /translation="LLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLR
                     FGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVV
                     TFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQKLAGAL
                     GMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEERALLIE
                     RSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSRSPQ
                     ITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            979..2445
                     /gene="mdtP"
                     /locus_tag="OO572_002061"
     CDS             979..2445
                     /gene="mdtP"
                     /locus_tag="OO572_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6661066.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            2643..2882
                     /locus_tag="OO572_002062"
     CDS             2643..2882
                     /locus_tag="OO572_002062"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661067.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            2892..3062
                     /locus_tag="OO572_002063"
     CDS             2892..3062
                     /locus_tag="OO572_002063"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6661068.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            3111..4523
                     /gene="fdhF"
                     /locus_tag="OO572_002064"
     CDS             3111..4523
                     /gene="fdhF"
                     /locus_tag="OO572_002064"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6661069.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            4524..4790
                     /locus_tag="OO572_002065"
     CDS             4524..4790
                     /locus_tag="OO572_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6661070.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            4884..5573
                     /locus_tag="OO572_002066"
     CDS             4884..5573
                     /locus_tag="OO572_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6661071.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(5651..6964)
                     /gene="gltP"
                     /locus_tag="OO572_002067"
     CDS             complement(5651..6964)
                     /gene="gltP"
                     /locus_tag="OO572_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6661072.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(7306..7902)
                     /gene="nrfG"
                     /locus_tag="OO572_002068"
     CDS             complement(7306..7902)
                     /gene="nrfG"
                     /locus_tag="OO572_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6661073.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(7899..8282)
                     /gene="nrfF"
                     /locus_tag="OO572_002069"
     CDS             complement(7899..8282)
                     /gene="nrfF"
                     /locus_tag="OO572_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6661074.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(8275..9993)
                     /locus_tag="OO572_002070"
     CDS             complement(8275..9993)
                     /locus_tag="OO572_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6661075.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(10013..10969)
                     /gene="nrfD"
                     /locus_tag="OO572_002071"
     CDS             complement(10013..10969)
                     /gene="nrfD"
                     /locus_tag="OO572_002071"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6661076.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(10966..11637)
                     /gene="nrfC"
                     /locus_tag="OO572_002072"
     CDS             complement(10966..11637)
                     /gene="nrfC"
                     /locus_tag="OO572_002072"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6661077.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(11634..12200)
                     /gene="nrfB"
                     /locus_tag="OO572_002073"
     CDS             complement(11634..12200)
                     /gene="nrfB"
                     /locus_tag="OO572_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6661078.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(12245..13681)
                     /gene="nrfA"
                     /locus_tag="OO572_002074"
     CDS             complement(12245..13681)
                     /gene="nrfA"
                     /locus_tag="OO572_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6661079.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            14074..16032
                     /gene="acs"
                     /locus_tag="OO572_002075"
                     /pseudo
     CDS             14074..16032
                     /gene="acs"
                     /locus_tag="OO572_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            16232..16546
                     /locus_tag="OO572_002076"
     CDS             16232..16546
                     /locus_tag="OO572_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6661080.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            16543..18192
                     /gene="actP"
                     /locus_tag="OO572_002077"
     CDS             16543..18192
                     /gene="actP"
                     /locus_tag="OO572_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6661081.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            18370..19662
                     /locus_tag="OO572_002078"
     CDS             18370..19662
                     /locus_tag="OO572_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6661082.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(19816..21465)
                     /locus_tag="OO572_002079"
     CDS             complement(19816..21465)
                     /locus_tag="OO572_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6661083.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(21616..22965)
                     /gene="ghxP"
                     /locus_tag="OO572_002080"
                     /gene_synonym="yjcD"
     CDS             complement(21616..22965)
                     /gene="ghxP"
                     /locus_tag="OO572_002080"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6661084.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(23671..24135)
                     /gene="soxR"
                     /locus_tag="OO572_002081"
     CDS             complement(23671..24135)
                     /gene="soxR"
                     /locus_tag="OO572_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6661085.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            24221..24544
                     /gene="soxS"
                     /locus_tag="OO572_002082"
     CDS             24221..24544
                     /gene="soxS"
                     /locus_tag="OO572_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6661086.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(24547..26124)
                     /gene="pdeC"
                     /locus_tag="OO572_002083"
                     /pseudo
     CDS             complement(24547..26124)
                     /gene="pdeC"
                     /locus_tag="OO572_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            26553..26834
                     /locus_tag="OO572_002084"
     CDS             26553..26834
                     /locus_tag="OO572_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6661087.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         6804 a         7171 c         6762 g         6106 t
ORIGIN      
        1 attgctcgcc tgtttgatct gttacacctt ttacagcggc gtggactggg aaggcattca
       61 cacctgtatg ctgacctgcg tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa
      121 gatggtgctg cgttttggcg gggccttttg cggcgcgatt ctggcgctgt tattcacgct
      181 actggtcatg ccctggctgg acaatattgt cgaattgctg tttgtgctgg caccgatttt
      241 cctgttgggc gcatggattg ccaccagctc tgaacgctct tcttatatcg gcacacagat
      301 ggtggtcacc ttcgcgctcg ccacgctcga aaacgttttt ggcccagtgt acgacctggt
      361 ggaaattcgc gatcgcgccc tgggtatcat cattggtacc gtggtgtccg cgatgattta
      421 cacctttgtc tggcctgaaa gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct
      481 gggtatgtta agtaaagtaa tgcggatccc acgccagcag gaagtcacgg ctctgcgcac
      541 ttatctgcaa attcgtatcg gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca
      601 acgcgtggcg ctggagcgtc aactggacag cgaagaacgc gccttactga ttgaacgttc
      661 gcaaacggtt attcgtcagg gccgcgatat ccttcacgcc tgggatgcca cctggaattc
      721 ggcgcaggcg ctggataacg cactacagcc ggacagagca ggtcagtttg ccgacgccct
      781 ggagaaatac gctgccggtc tggcaaccgc actcagccgt tctcctcaaa taacgcttga
      841 agagacaccc acctcgcagg ccatcctgcc caccttatta aaacaggagc aacacgtctg
      901 ccagcttttc gcccgcttgc cagactggac agccccggca ttaacgcccg ccacggaaca
      961 ggcacaagga gccacgcaat gatcaatcgt caactttcac gtctgctgtt gtgcagcatt
     1021 ctcggcagca cgacgctgat ttccggctgt gccctggtac gtaaggattc tgcgcctcat
     1081 caacagctca aaccggaaca aatcaaactg gccgatgata ttcatcttgc cagctccggc
     1141 tggccgcagg cgcagtggtg gaaacaactc aatgacccgc agttggatgc cctgatccaa
     1201 cggacgctaa gtggttcaca caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa
     1261 tcgcaggccg atttgttaga tgccggttca cagttgcagg tggcagcgtt agggatgctt
     1321 aaccgccaac gtgtctcggc gaacggcttt ttaagccctt atgcgatgga tgcgcccgca
     1381 ctggggatgg acgggccgta ctatacggaa gccacagtag gtttgtttgc cggactggat
     1441 cttgatttgt ggggtgtgca tcgctcagcg gttgccgccg ccattggcgc gcataatgcc
     1501 gcgctggcag aaaccgcagc agtagagcta tcgctgacca cgggcgtagc gcagctttat
     1561 tacagtatgc aggccagcta tcagatgctc gatctgttag aacaaactcg cgatgtgatt
     1621 gattacgcgg tgaaagcgca ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct
     1681 ttccacggcg cgcgggcaca gattctggcg gtcgataaac aaattgctgc cgtcaaaggg
     1741 caaatcaccg aaacgagaga atctctgcgc gcattgattg gcgcgggagc cagcgatatg
     1801 ccggagatca aacctgtggc attaccacga gtccagaccg gcattccggc gacactctct
     1861 tatgagttgc tcgccagacg cccggatctg caagccatgc gctggtatgt tcaggcgtca
     1921 ttagatcagg tggattccgc gcgggcgttg ttctatccga gctttgatat caaagcgttt
     1981 ttcggccttg atgccatcca cctggatacc ttattcaaaa aaaccagtcg ccagttcaac
     2041 ttcatcccgg gtctgaaatt gccgctgttt gacggtggac ggttgaatgc caatctcgaa
     2101 ggcacgcgcg ccgccagcaa catgatgatt gaacgttaca accagtcagt actgaacgcg
     2161 gtgcgtgacg ttgccgtcaa cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg
     2221 caggctgaac acgtggaagc aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag
     2281 cgcggcttaa ccagccgctt acaggccact gaagcccggt tgccagtact tgccgaagag
     2341 atgtcattac tgatgctgga cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg
     2401 ctgggcggcg ggtatcaggc ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg
     2461 atggctgtca cgcggtattt cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt
     2521 cagagttttg aataaatagt gcccgtaata tcagggaatg accccaaata aaatgtggca
     2581 taaaagatgc atactgtagt cgagagcgcg tatgcgtgat ttgattaact ggagcgagac
     2641 cgatgaaaaa agtcgtcacg gtttgcccct attgcgcatc aggttgcaaa atcaacctgg
     2701 tcgtcgataa cggcaaaatc gtccgggcgg aggcagcgca ggggaaaacc aaccagggta
     2761 ccctgtgtct gaagggttat tatggctggg atttcattaa cgatacccag atcctgaccc
     2821 cgcgcctgaa aacccccatg atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct
     2881 gagatgaggc actgaattac gttgccgagc gcctgagcgc catcaaagag aagtacggtc
     2941 cggatgccat ccagacgacc ggctcctcgc gtggtacggg taacgaaacc aactatgtaa
     3001 tgcaaaaatt tgcgcgcgcc gttattggta ccaataacgt tgactgctgc gctcgtgtct
     3061 gacacggccc atcggttgca ggtctgcacc aatcggtcgg taatggcgca atgagcaatg
     3121 ctattaacga aattgataat accgatttag tgttcgtttt cgggtacaac ccggcggatt
     3181 cccacccaat cgtggcgaat cacgtaatta acgctaaacg taacggggcg aaaattatcg
     3241 tctgcgatcc gcgcaaaatt gaaaccgcgc gcattgctga catgcacatt gcactgaaaa
     3301 acggctcgaa catcgcgctg ttgaatgcga tgggccatgt cattattgaa gaaaatctgt
     3361 acgacaaagc gttcgtcgct tcccgtacag aaggctttga agagtatcgt aaaatcgttg
     3421 aaggctacac gccggagtcg gttgaagata tcaccggtgt cagcgccagt gagattcgtc
     3481 aggcggcacg gatgtatgcc caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa
     3541 cccagttcta ccagggcgtg gaaaccgtgc gttctctgac cagcctcgcg atgctgacca
     3601 gtaacctcgg taagccgcat gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg
     3661 gtgcctgcga tatgggcgcg ctgccggata cgtatccggg ataccagtac gtgaaagatc
     3721 cggctaaccg cgaaaaattc gccaaagcct ggggcgtgga aagcctgcct gctcataccg
     3781 gctatcgcat cagcgagctg ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca
     3841 ttatgggcga agatccgctg caaactgatg cggagctgtc ggcagtacgt aaagcctttg
     3901 aagatctgga actggtcatc gttcaggaca tctttataac caaaaccgcg tcggcggcgg
     3961 atgtcatttt gccgtcaacg tcgtggggcg agcatgaagg cgtgtttact gcggctgacc
     4021 gtggcttcca gcgtttcttc aaagcagttg aaccgaaatg ggatctgaaa acggactggc
     4081 aaatcatcag tgaaatcgcc acccgtatgg gttatccgat gcactacaac aacacccagg
     4141 agatctggga tgagttgcgt catctgtgcc cggatttcta cggtgcgact tacgagaaaa
     4201 tgggcgaact gggcttcatt cagtggcctt gccgcgatac ttcagatgcc gatcagggga
     4261 cttcttatct gtttaaagag aagtttgata ccccgaacgg tctggcgcag ttcttcacct
     4321 gcgactgggt agcgccaatc gacaaactca ccgacgagta cccgatggta ctgtcaacgg
     4381 tgcgtgaagt tggtcactac tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg
     4441 cgctggctga tgaacctggc tacgcacaaa tcaataccga agacgccaaa cgtctgggta
     4501 ttgaagatga ggcattggtt tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc
     4561 aggtcagcga tcgtccgaac aaaggggcga tttacatgac ctaccagtgg tggattggtg
     4621 cctgtaacga gctggttacc gaaagcttaa gcccgattac gaaaacgccg gaatacaaat
     4681 actgcgccgt gcgcgtcgag ccgatctccg atcagcgcgc cgccgagcag tacgtgattg
     4741 acgagtacaa caagttgaaa actcgcctgc gcgaagcggc actggcgtaa tacagtcctt
     4801 tctacagcct cctttcggag gctgtttttt tatccattcg aactctttat actggttact
     4861 ccctacccaa tcgtattatc aaaatgaaaa aaattatcgc attgatgttg tttttgacat
     4921 tctttgccca cgcgaacgac tccgagcctg gcagccagta tttaaaggca gcagaggccg
     4981 gggaccgacg tgcacaatat tttcttgccg acagctggtt tagctccggc gatttgagca
     5041 aagccgaata ttgggcacag aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt
     5101 tggcgcagat caaaatcacc aatccggtca gtctggacta tccacaagca aaagttcttg
     5161 cagagaaagc ggcgcaagcg ggcagtaaag aaggtgaagt aacgctggcg catattctgg
     5221 taaatactca agcgggtaaa ccggattatc caaaggcgat ttcgctgtta gaaaacgcct
     5281 cggaagatct ggagaatgac tccgccgtcg atgcccaaat gctgcttggt ttgatttacg
     5341 ccaacggcgt gggcattaag gccgacgatg acaaggcaac ctggtatttc aaacgcagct
     5401 ctgcaatttc ccgaaccggt tattccgagt actgggcggg aatgatgttc ttaaacggtg
     5461 aggaaggttt tatcgagaag aacaaacaaa aggcgctgca ctggttgaac ctgagctgta
     5521 tggaagggtt tgataccggg tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc
     5581 ctgataagac gcggcaagcg tcgcatcagg catccacaca ttgccggggg atatccccgg
     5641 caatcttcaa ttattgatcc gcagttttat caaatttgcc cagcacttca cgctcataag
     5701 ccagcgcctt tttgcggtca aatttgtgtt cccacttggc aatcaccagc accgccagcg
     5761 cattacccac cacgttcagc gcagtacgcg ccatgtcgag gatacggtca acaccagcaa
     5821 taaacgccag accttccagc ggaataccta cgctacccag cgttgccagc aacaccacaa
     5881 acgacacgcc agccacgcca gcaatccctt tcgaggtcac catcagcgtc agcaccagaa
     5941 tgatttcctg ccagatggac aggtcaatgc catacagctg agcgatgaaa atagcggcaa
     6001 tactttgata cagcgtcgaa ccatcaaggt taaaagagta accggtcggc accacgaagc
     6061 tggtgatcga cgccggtgct ccgtaggctt ccatcttctc aataattcgc ggcagtacgc
     6121 tttcagagct ggcagtggag tacgccagaa tcagctcatc tttcagaata cgaatcagga
     6181 tccagacgct taacccgcac agacgtgcca caattcccag cactaccagc gcgaagaaca
     6241 gaatggcgaa atgcaccagc agcaccagtt tcgccagtgg ccacagagac gagaaaccaa
     6301 agttagccac cgtcaccgca atcagcgcaa acacacccac cggtgcataa cgcatcacca
     6361 tgtgagtcac tttaaacatg gtttcagaga tggagcggaa cacggtcacc agcggttcac
     6421 gatgcgtcgc gggcagggaa gaaagcccca gaccaaacag caccgagaaa aagatgatcg
     6481 gcagcatttc gcctttcgcc atcgacgcca caatgttcgt cggcaccagc gacaaaatcg
     6541 tgcccataat gccgtgggaa ctgctttgta ccgcttccgt agtgctctga tatttcgaga
     6601 tatcgacggt cgccagctgc gacatatcca ccccggcacc gggctggaag acgttcgcca
     6661 gagtgatccc caaaatgatg gcgacggtgg tgatcacctc gaagtaaata atggttttcg
     6721 cgccaatacg cccgagctgt ttggcatcac caacacccgc gatacccacc accagcgtgg
     6781 agatcacaat cggcacaaca atcattttga tcagatggat gaagatatca cccgccggag
     6841 agagcaaatt gacgaccagc cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca
     6901 ggagaatgcc cagcaccata gcaaacagaa tctgccaggc caggctgaat tttatatttt
     6961 tcatcagaat aacttcctca atggagcgcc gttccccgtt cagaaacgcg agaacgtgac
     7021 atactgccag ggcatgaatt gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc
     7081 agtatcattt gcgcggtatg ttgcgcaacc ctttaacaac agagcttttc gctgcattaa
     7141 tgcataataa cgcgagttta tggcaggatt aatgaataac catttgttat ggaaagattt
     7201 ttatttgttt aatctgcaat aatactgatt ggtgattatt tttggcatat gttcattcga
     7261 ttatttttta tgcagctcaa tgacataatc ttcatcacca gatgattaat ccgatctccg
     7321 ctgcaacagt tttgccatat taatcgactc gaccagctgt gtccggttga tccggggtga
     7381 gttgagatcc atcacttttt gccatagttc gatggcttgc gcgtaattcg cctgcataaa
     7441 cgcatcggaa gccagcagca tcagagcggt gatttcgtta gagtccagcg cgagggcttt
     7501 gtcgatcatt gcgcgagtct gggcggtcat atgctggctg gcctggtaat aaagcaccgt
     7561 cgccagcgcc gcatacagtt cagcgttctc accacgtagt tgcaacgcct gacgatacgc
     7621 cagcagcgaa ttgctgtaat cgttttgcca cagatagtac tcgcccagta acgcccattg
     7681 ttcgctgttt tgtggattag cgcggatttt atcctgcaat gcctgaagct gcgcttccgg
     7741 ggtttgctgg ctggcaaact gatgtagcgg atcgcgctga cgctgatact ccgcacgtac
     7801 cgcctgccat tttgggctta acagataact gccgacacag aggaatacca tcaatatcgt
     7861 gagtgttgtc agcattttca ccggtatttg aggctgtttc atcgcttagc cctcactcgc
     7921 cagaggatca gtgccatcag caataacaac accactggca gcgcccacaa caccagcgtc
     7981 tgacccgtta acggtgggtt atagcgaaca aaatctccgt agcgttcggt catccagcca
     8041 atgatttcga cttcgttctt cccctccgac accatgctat aaacctgatg gcgcatactg
     8101 accgccaccg gcgcattgga ttcgagtaag ttttgattct ggcactgcgg acaacgtaac
     8161 tggctggcaa tatttaacgc ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct
     8221 acaacctgag cgcgcgcaaa acaggtaaat aacagcagta gcgtgagaag ccctttattc
     8281 atcgcgcttc ttcccccgcc atccgcttaa caatgcgccc gcaatcatca acaatcctcc
     8341 cccccagatc cagcgcacac cgctttgtac atacaaacga aaagcgtaac gatccgctct
     8401 ggttttttca cccatgaccg cataccagtc atggatgccg ttccagcgaa ttgacggttc
     8461 catcatttgc tgacggcgtg cttcataaaa acgccgctcc ggcgttaatt caccaatgcg
     8521 ttgctgatgg tcaaacagcg ccactatcgc tttttcgctg gtgtaattgc ctctggcttg
     8581 cagatcgagt cgctcaaaac ggaaggtgta tcctgccagc gtcacctgct gacccggctg
     8641 taaattcagg ctgatctcct gacgactgac actggaaacc acgatccccg cggcaaataa
     8701 cagcacgcca gcatgagcta ccagcgccgg aagctgcacg cgtttgccag agacaaacgt
     8761 cgccagcaca atcaccacca gcatcaacag accaaacggt aacgtcgcgc ggttaaaata
     8821 cggcgcgcca acggagaggc gtccccagcc cagcaggccg taaatcatcg gataaagcgt
     8881 tcccaccagc acgatcagta gcactgcgca aaacagcaac agcgtagcga ggattaacat
     8941 ttcccgcgat aaccccgaaa aacgcaccgc cgggccaccg tcccgcgctc gccagccata
     9001 cagagccaaa gacgcaaggc taatcagtgc aaacaggctg aacaacggca cggcgcggac
     9061 gttatccagt gcgaacgcat gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc
     9121 cagcagcgac agcatcagag taactatcgc cagcaacagc gaccagtggc ggaaaatccc
     9181 ccgctggcgt gtcagggaca aactgtgcag cagcgcagtg gcagaaagcc agggtaataa
     9241 agaggcgttt tccaccggat cccagaacca ccagccgccc cagccgagtt cgcagtaggc
     9301 ccaccaggaa ccgaggatga tccccgccgt taatgcactc cagccaggta acgcccagcg
     9361 ccagcaaatt cgggcgctgg ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac
     9421 gctcgccgct accatcaaac cgccatagcc aaggtaaagc agcggtggat gaaagataag
     9481 accgggatgt tgcagcatcg gattgagatc gcggccttcg attgctggtg gaaatatccg
     9541 cacgaaggga tcggaccaca gcaccacaaa cagtagcagt gcggcgagca tgagagataa
     9601 aacggctagc gtcagcggaa atagcggatc gctttgctgc cgataatgcc aggcaaacag
     9661 cgcgctccag gcggaaagca gcagcaccca aagcagcagc gaaccttcat gaccgcccca
     9721 caccgccgcc agtttgagtt cccacgacag caggctgtag ctatgttggg cgacataaat
     9781 gaccgagaaa tcgctgatga gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag
     9841 cgcgaactgc gccagaatgc cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg
     9901 cactcccgca aaggccgtca acggggtcaa cacgttgacc ccgagactta acaacaacgc
     9961 cagaaaaccg acttcaggaa ggaatacgtc cagaaaccac ctcttttctg gcttacgcca
    10021 ccgtcaactg tccggcgtag agaatgaaaa agcgcagcat caacacgccg accagactgg
    10081 ccccacacgc cgccagcacg gcaggaatgc cggaactgcg attgacccac ggtttgagca
    10141 acattggcac aatcagcccc agcccggcga caccaagcca gaaccaccac gtccagaaac
    10201 cgccacctaa tgccgccacc agcgcacgaa ctttaccgtc atcgcccagc gccagaccga
    10261 caaaaaacgc caccagcagg aagatttcac cccataccac ggggatttcc atccggtgaa
    10321 caaagtgcgc ttccgtggaa tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg
    10381 ccaccgccgc accggacgag atgccggaga agaggagcag caccggcagg ctcgggttat
    10441 tgaggaacgg atacgatttc agcgccgaca gcagaaagcc ggtataagcc cccagcagca
    10501 ccgccaacac cagcatcagc gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa
    10561 ccttttgcac gatccccagc ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt
    10621 caaagatttt cgccagccac agcaccagca ccaccatgta gagctgaaac agcatcaccc
    10681 ccatcgacat caccgaggta aaactgtagt ggaacatcag cttccagaag gtccacggtc
    10741 ttgtcaggtg gaagacgagg atcaacagac cgaggatcac cgcgcccggc ccgacaatca
    10801 gcgtggtgcg cagcaacgta ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca
    10861 gcacggccag cgtcaccaga ccggcagaaa taccaatcaa aaacaggtag atggcaatcg
    10921 gccagtccca caccagcgat tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc
    10981 cccgtattta aacggtacgc ggtacagctt cggtttggtt cccagcgcca gcttgtagcg
    11041 gtaagtaggc ttctggcgca gcagttgcga aatctcactg ttaggatcgt ccagattgcc
    11101 aaacgtcagc gccttcgtcg ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa
    11161 attggtctta cgacagaaat cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg
    11221 cacgcggtac ggacaggcgg cgatgcagta ctgacaaccg acgcagagat caggattaac
    11281 atcgacaatc ccgctagcgg catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca
    11341 cggcgcatga tcgcagtgct ggcaagactt acggaagaag cgatatttca catcaggaaa
    11401 ttcgccctgc ggctcgctac gaataatcgt caggcgcgag acgccttccg gcaccttgtt
    11461 cacttcccga caagcatcca tacaggcggt acagccgatg cataacgact cgtcatgcac
    11521 catgccgtaa cgcaccccat tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc
    11581 gctgacggct gccagcacgc cgacgccggt gagaaactgg cgacgagacc aggtcatggc
    11641 tgctccttaa gcaacggaac agacgccggg ttaaagttcg gattggtgcg ctgatcgctg
    11701 tggcaatcga cgcaaatctt aatccgtcct ttgtcactta acgtctgcat cgtatcttgc
    11761 tgtggatgca gggaatggca gctggcgcac gccactttgg ttacgtggac atcgtgcggc
    11821 cagaacgctt tttgcaactg ttccggcaga tgacaggaca tacagacgct gttctgctct
    11881 ccgaccttat acatcggctc gttaaagcgc atcacatctt tcaccccttc gcggtgttgc
    11941 ggtgatgctt ggccgtgaca gttggtgcag gtgaccggca atttgttatt cgggttgata
    12001 acggaagcat gtttgccatg cataccttcg gtatctggtt tatgacagtc cagacaggcg
    12061 gcatccgggt tacgctgctg ggtaacttcg tagcgatcac cagatgcctg caccgcaggg
    12121 gtagcggtaa ttccgttcag gctccacaac aggcccgacg ccagcacccc agcagttaat
    12181 aacgaacgta atacgctcat attcactcca ttgtgttttc cgccccttgc gaggcggaac
    12241 ggggttattg gcttaacaga ccgtttttac gtgcctgctc ttcccactgc gggatcaccg
    12301 ttttgatgaa gtcctgcttc tcggccttga tttgttccat gttcaggcca atggcctgct
    12361 gggctttctc tttggtcgag atatccggga tctggatttc atgggtgata cctttggtcg
    12421 ccagcaggcg cgccagtttg gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca
    12481 tccgtagacc ttcttccggt gcgtgcatat gaataccgtg ggaagcaatc gccagatccc
    12541 aacgccactg ggcatgacgg atatcgtcct gaatcggctt catttcggct tccgtcgcgc
    12601 ctgcatccag cgccgctttc gcttcgaagt gagcgtgaac cagttgatct tcaaccttga
    12661 ttttcaggtc gttaatcgat tgcttacgtt ccgcgaccac tttttgcaag gcagctttat
    12721 cctgggtatg gcagttcgca caagtctggg cgaagttatc aaacggatta ccaattttat
    12781 ggtcggtgta gagtttgcct tcggcgttct gcacttttgg catatggcag tcgatacagg
    12841 tcacgttgtt tttaccgtga atgcccgctg tccaggtttc atattccggg tgctgcgctt
    12901 tcagcattgg cgttttcgac agggagttag tccagtcaga gaaggcaatt ttgtcgtaat
    12961 actgctccat attttcgact ttcatgccgt catcccacgg gaatttaacc gctttgtttt
    13021 tgccgtcgaa gtaatactcc acatggcact gaccgcaaac catcgattgc tggtcgaaac
    13081 gtccggcttt ctcaaaaggt ttaccaatgg cttccatcgc gcgagccgca tacggacggg
    13141 aaagggttaa ctccggtttg cctttggcga actctggtga ggcggtgtta tggcagtcgg
    13201 cacaacctaa gttgttgacg atttccgggc cgccgcgcgc ccatttaccg tggaagtagc
    13261 catcttcgcc gtctttctgg atcagacgcg ccacatccgg gcttttacaa ctccagcacg
    13321 ccatcggtag cggaccatct tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca
    13381 catcggtcac agcaaaggca tgtccacgag gcttgttgta atcgcgcgag aagggatacc
    13441 ccgcccacag gatcaccagc cgtggatctt ccgccagggc gtcaacacgc tctgactgtt
    13501 ccgaggtggc tttccaggag agatattgat cgtgatgctg cggggcaaag gtttcattct
    13561 tcgcttccac agttacaggt tttgcgggag ctgccgtttg ttcagcgtga acagaagtga
    13621 aaaagaaaaa aggaatcaat aagctgaaga tacggcgtgc gtttattttt atccttgtca
    13681 taggggcttc atccgaattg cgccattgtt gcaatggcgg tttttattgt ttttcacgac
    13741 agtaaccgca cctacactgt catgacattg ctcaccccta tgtgtaacaa ataaccacac
    13801 tgtgaatgtt gtctttaatc aattgtaagt gcatgtgaaa taccacttta gagttagtca
    13861 gtatcttcct ctttttcaac agcatgcata actgcatgtt cctcaaagaa ttaatcaact
    13921 tttgttggtg accttcaaaa attaccctgc cgtttatttg caccattcta cttttgcgtg
    13981 atctgtcgcc caaatactaa acaaaactgc caatacccct acatttaacg cttatgccac
    14041 atattattaa catcctacaa ggagaacaaa agcatgagcc aaattcacaa acacaccatt
    14101 cctgccaaca tcgcagaccg ttgcctgata aaccctcagc agtacgaggc gatgtatcaa
    14161 caatctatta acgtacctga taccttctgg ggcgaacagg gaaaaattct cgactggatc
    14221 aaaccgtacc agaaggtgaa aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg
    14281 tacgaggacg gcacgctgaa tctggcggca aactgccttg accgccatct gcaagaaaac
    14341 ggcgatcgca ccgccatcat ctgggaaggc gacgacgcca gccagagcaa acatatcagc
    14401 tataaagagc tgcaccgcga cgtctgccgc ttcgccaata ccctgctcga gctgggcatt
    14461 aaaaaaggtg atgtggtggc gatttatatg ccgatggtgc cggaagccgc ggttgcgatg
    14521 ctggcctgcg cccgcattgg cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa
    14581 gccgttgccg ggcgcattat tgattccaac tcacgactgg tgatcacttc cgacgaaggc
    14641 gtgcgcgccg ggcgtagtat tccgctgaag aaaaacgttg atgacgcact aaaaaacccg
    14701 aacgtcacca gcgtagagca tgtggtggta ctgaagcgta ctggcggaaa catcgactgg
    14761 caggaagggc gcgacctgtg gtggcacgac ctggttgagc aagccagcga tcagcactag
    14821 gcggaagaga tgaacgccga agatccgctg tttattctct atacctccgg ttctaccgga
    14881 aaaccaaaag gcgtactgca cactaccggc ggttatctgg tgtacgcggc gctgaccttt
    14941 aaatatgtct ttgattatca tccgggtgat atctactggt gcaccgccga tgtgggctgg
    15001 gttaccggac acagttattt gctgtacggc ccgctggcct gcggcgcaac cacgctgatg
    15061 tttgaaggcg taccgaactg gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat
    15121 caggtcaata ttctctatac cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat
    15181 aaagcgattg aaggcaccga ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca
    15241 attaacccgg aagcgtggga gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg
    15301 gtcgatacct ggtggcagac cgaaaccggc ggtttcatga tcaccccgct gcctggcgct
    15361 accgagctga aagccggttc ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc
    15421 gataacgaag gtaacccgct ggaaggggct accgaaggta gcctggtgat caccgactcc
    15481 tggcctggtc aggcgcgtac gctgtttggc gatcacgaac gttttgagca gacctatttt
    15541 tccaccttca aaaatatgta tttcagcggc gacggcgcgc gtcgcgatga agatggctat
    15601 tactggatca ccgggcgtgt ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg
    15661 gcagagatag agtcggcgct ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt
    15721 attccgcaca atattaaagg tcaggcgatc tacgcctatg tcacgcttaa tcacggggag
    15781 gaaccgtcac cagaactgta cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg
    15841 ctggcgacgc cagacgtgct gcactggacc gactccctgc ctaaaacccg ctccggcaaa
    15901 attatgcgcc gtattctgcg caaaattgcg gcgggcgata ccagcaacct gggcgatacc
    15961 tcgacgcttg ccgatcctgg cgtggtcgag aagctgcttg aagagaagca ggctatcgcg
    16021 atgccatcgt aacccacaat tgccggatgc gaatcggtaa cggtttgtag gcctgataag
    16081 acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc
    16141 tacattcggc aagggttacc cgagcgttaa ccttctccca taagggagcg ggaattaaaa
    16201 caatccctac attacctctg gagaatctgt gatgaatggt acgatttatc agcggataga
    16261 agacaatgcg catttcaggg agttagtcga aaaacggcaa cggtttgcca ccatcctgtc
    16321 gattattatg ctggcagttt atatcggctt tattttactg atcgccttcg cgcccggctg
    16381 gctgggcacg ccgctgaatc cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg
    16441 agtgattgtg atctcctttg ttctcaccgg tatctacatc tggcgggcga acggcgaatt
    16501 cgaccgtctt aataatgaag tcctgcatga ggtacaagca tcatgaaaag agttctgacg
    16561 gcgcttgccg ccacactccc tttcgcagct aacgccgcgg atgctattag cggggccgta
    16621 gagcgccagc caacgaactg gcaggcgatt attatgttcc tgattttcgt cgtgtttacg
    16681 ctcggcatta cctactgggc atcaaaacgc gtacgttctc gtagcgacta ctacaccgca
    16741 ggcggcaata tcactggctt ccagaacggg ctggcgattg ccggggacta tatgtccgcc
    16801 gcctcattct tggggatctc cgcgctggtg tttacctccg gctatgacgg gctgatctac
    16861 tcgttgggct tcctggtggg ctggccaatc attctgtttc tgattgccga acgtctgcgt
    16921 aacctgggac gctacacctc tgccgatgtg gcctcttatc gtctgaaaca agggccgatt
    16981 cgtattcttt cggcctgtgg ttctctggta gtggtggcgc tttaccttat cgctcagatg
    17041 gtaggcgcag gtaaactgat cgagctgctg tttggcctta actatcacat tgcggtggtg
    17101 ctggtcggcg tgctgatgat gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg
    17161 gtacaaatta tcaaagctgt gctgttgctg ttcggtgcca gctttatggc ctttatggtg
    17221 atgaaacacg tcggctttag cttcaacaat ctgttcagcg aagcgatggc ggtacacccg
    17281 aaaggtgtcg acatcatgaa accgggcgga ctggtgaaag atccgatctc cgcgctctct
    17341 ctggggctgg gactgatgtt tggtacggcg ggcttgccgc acattctgat gcgcttcttt
    17401 acagtcagcg atgcccgcga agcacgtaag agcgtgttct acgccaccgg atttatgggc
    17461 tacttctata ttctgacctt tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat
    17521 ccggaatata aagacgcggc gggccatctg attggtggta acaacatggc ggccgttcac
    17581 ctggcgaatg cagtgggcgg caacctattc ctcggtttta tttcagcggt tgctttcgcc
    17641 actatcctcg cggtggttgc ggatctgacg ctggcgggcg catcggcggt ttcgcatgac
    17701 ttgtacgcta acgtcttcaa aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa
    17761 atcaccgtac tgatcctcgg cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag
    17821 aacatcgcct ttatggtagg ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc
    17881 attctgcttt ccatgtactg gtcgaaactg actacccgtg gcgcgatgct gggcggctgg
    17941 ctggggctga ttaccgcagt ggtgctgatg atcctcggcc cgactatttg ggtacagatc
    18001 ctcggtcacg aaaaagccat cttcccgtat gaatacccgg cgctgttctc tatcagcgtg
    18061 gcattcctcg gcatctggtt cttctcggca accgataact cagcggaagg ggcgcgcgag
    18121 cgtgaactgt tccgcgcgca gtttatccgc tcccagaccg gctttggcgt tgagcaaggc
    18181 cgcgcacatt aatctctccc cttccccggt cgcctgaccg gggaatacgc ttcctctcca
    18241 acatgcatca ccttttccca taatattaaa caaataaact cattaaaaaa tgagcgattt
    18301 ttgacagtcg tagaaaatga taatgcagag aatatgcctt ttctttcttg ttaattataa
    18361 ggatatttta tgcgctataa tggtttaaat aatatgtttt tccctctttg ccagattaac
    18421 gataaccact ctatcacaag ttcatcacat acaaagaaaa caaaatcaga taattacagc
    18481 aaacatcata aaaacacgtt aattgacaat aaagccctct ctcttttcaa aatagatgat
    18541 catgaaaaag tgataggctt gattcagaaa atgaaaagaa tttatgatag tttaccatca
    18601 ggaaaaatca cgaaagaaac ggacaggaaa atacataaat attttataga tatagcgtta
    18661 tatgcaaata ataaatgtga cgatagaatt acgagaagag tttaccttag taaagaaaag
    18721 gatgtatcca ttaaggtggt atattttata aataatgtcg ccgtccataa taatactatc
    18781 gaaattccac agatagtaaa tggtggttac gatttttcac accttagcct gaagggtatc
    18841 gtgattaaag atgaagattt atccaattcg aattttgcag gttgcagact acaaaacgct
    18901 attttccagg actgtaatat gtataaaacg aatttttatt acgccataat ggaaaaaata
    18961 ctttttgata attgtattct cgatgactca aatttcgctc agataaaaat ggccgacgga
    19021 actctaaatg catgctccgc tatgcatgtt caattctaca atgcagcaat gaatagagcc
    19081 aatattaaaa ataccttcct tgactattca aatttttata tggcgtacat ggctgaggta
    19141 aatctttata aagtaatagc gccatatgtt aatttattta aagccgacct tagtttctct
    19201 aaactcgatt taattaactt tgaacatgct gatctgtctc gcgtcaatct gaacaaagca
    19261 atcctccaga atataaactt aattgatagc aaactctttt gtacgtggct gacaaataca
    19321 ttcctcgaaa tggttatatg taccggctct aatatggcta atgttaattt taataatgcc
    19381 aatttaagca actgccattt caactgttct attttaacaa aagcctgtat gtttaatacc
    19441 cgtctctatc gggttaattt cgatgaggct agcgtccagg gaatgggcat ttccattctc
    19501 cgtggggagg aaaatatccc cattgatagt gataccctgg taacactaca gaaattcttt
    19561 gaagaagatt gtacctctca tactggcatg tcacaaactg aggataatat taatgcagtc
    19621 gctatgaaga ttactgcaga tattatgcaa cacgcagatt gaggttaagt cgaagaacag
    19681 ccaatgaatt gactttacaa gagagataac ttatttgttt tagaatcatg aatgttcgcc
    19741 cgagctaacc tgttaaaggt gcaaaagatt gcaccttttt cagattacct cgggcctggt
    19801 tacgtcgctt tcggcttact gattttcctc aattagcaac gcttcaagca aatcgagatc
    19861 gtgcagtaat ttttgcagcg tttcgttgct gatctcccgc gtggcgcgca ggtggtacag
    19921 ttcagcacgt tcagaacgca atgccgccag acggaagcga cgctcaaggt tctcttcctg
    19981 cacggaactt tcaacgtcgt tacgtccatc ggcgcgacga cgcaggttac caatgacgcg
    20041 agaactgacc tccgtaagca actggttatc gatattctct tcggtatccg ccgccagacg
    20101 ctcctccatt ttctggatcg ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg
    20161 ttcttctttc aattgttgcg aatggtccgc gacttcaatg tgttgtagca gaataggcaa
    20221 catcaccacg ccgacaaaca gcgagaagag aatgacgcca gccgccagga acaccagctc
    20281 atagcgcgcc gggaagacgt taccatccgg caagagcagc gggattgaga gcacaccggc
    20341 cagagtgatc gccccacgca ccccagcgaa agacgcgatc aggatttctc gtgtggtcca
    20401 cgaaccaaac tccatcggct tcttcttcag gaagcggttg ctgaactttt tcatcgtcca
    20461 cagccagccg aaacggacca gcatcagcgc cgcatatatc agaataatat cggtaaacag
    20521 catccagatt tcgacgttag ggtcgatttc tgccgccatc agcgacgtct ccagaatacc
    20581 cggcagttgc agacctaaca gcaggaacac catgccgtta aagacaaatt ccagcatcgc
    20641 ccaggtgctg tttgcgcgca ggcgcattgc cagtggcgca cggcgcatca caccggagcg
    20701 ggtgatggtc atcccggcgg caaccgcagc gaggatgccc gaaacgccaa tatgttcggc
    20761 aatcagataa gaagcgaatg gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc
    20821 accgccccag cggctgagga atcgcagcga gcgaccatac agccagctca ccacaaaacc
    20881 ggcaagaata ccgccaatgg ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa
    20941 gatcatcgtc cccatcgcca ctgccacggc aaacttcaac gacaccaggc cggaagcgtc
    21001 attcatcaac gcttcgccct gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc
    21061 aacaatcccg gagagcgcca cagcatcggt cggagaaagt accgccgcca gcgcaaaggc
    21121 ggggatcagc ggaatgcccg gcaccaccca gtaaataagg aagccaatgc cgaccacggt
    21181 gaccaccacc agcgccagcg cgaggccgaa aatctctcga ccatgttcga gaaattcacg
    21241 ggtcggcgtt ttccagccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag
    21301 ttcaggatca aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc
    21361 gatttgcata agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag
    21421 cgagaccacg agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc
    21481 gatgtttttt atgatctgag aaagacgtta tgcctgaatt ctcatttttt cagaatatcg
    21541 caaaagctca cacaatgtgc ggagaaaata aaaacgggca gccattgcca cccgttttca
    21601 gaatccgatg aaagattaga tagcccagcc acccgcatag aaggtcacca gcgccacggc
    21661 gataacgacc gtaccgatgt tcaacttgcg ccattcaccg gaaaccagac gaccaatcac
    21721 cagagtcgcg aagccgatca tgatgcctgt tacgatgtta caggtcagca cgatgaatac
    21781 cgccgtaacc agacccgcca tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt
    21841 gctcagcatc agcaggccaa cgtacatcag cgccggagcc gttgcatacc ccggaacgag
    21901 gtaagaaagc ggagagagga acagaatcag caggaacagc acgccaacgg tgatagccgt
    21961 caacccggtt ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg
    22021 agctgcaccc accaggccag agaaaacgct gctcatggag tcagtggtca gtgctttccc
    22081 gccgtcgatg atctgcccat ctttatccag caggttcgcc tggccggcga cagcgcggat
    22141 agttccggtg gcatcaaata ctgccgtcat caccagcgcc agaacgcttg gcaggactac
    22201 aggattcagc gcgcccataa tgtccaggct gccaatcagt gaattgccgt tttcatcgct
    22261 caatgaaggc atggcgaaaa cgccggagaa atggacgtta ggatcgaaga tcaaaccgac
    22321 aattgagata ccgataatgg tcagcagaat gccaccaggg actttcagtt tttccaagcc
    22381 gatgatcacc gccagacctg ccagtgacat aatcaccggg aaggtcgcga aatcacccag
    22441 cgcaacgggc agaccatcaa gcgggttttt aatcaccaga ccgacaccat tagcggcaat
    22501 gagcagcagg aacagaccga taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt
    22561 gcgcaaaatc cagctacgga tacccgtggc agaaattacc gtaaacagca cacccatcag
    22621 gaacacggca cccagcgcga caggtacgct aatatgttgc cccagcacca ggctgaatgc
    22681 ggtaaacgcc gtcagggaga tggcgcaacc aatcgccaac ggcagattag cccacagacc
    22741 catcacgata gaaccgagtc cggcaaccag acaggttgca acgaaaactg ccgcaggcgg
    22801 gaagcctgct ttacccaaca tacctggaac gacgatgacc gagtagacca tcgccagaaa
    22861 cgttgttaac ccggcaacca cttcctgacg gacagtgctt ccacgttgtg aaattttaaa
    22921 ccaggcgtcg agtgaaccgc cggtacgcgc tgatggcgta gacataagaa aacatcccct
    22981 gagagtttaa ttttcgtcaa gatgcgtggc ggacaatccc ctgccagcga aacgttatct
    23041 tcctgctcca ggtttgcggc aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg
    23101 catacaaaac gggtggtaaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc
    23161 aaccgaagtt tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg
    23221 atgcgcaaac gttatcgtcg atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct
    23281 ggattgcgat aaaaaatcgt ctgagatgac aaaaatattg cggctaaaaa gtatactttt
    23341 acactggcga agaaacgtcg gtgacataca attaaagcat caacaccaac cggaacctcc
    23401 accacgtgct cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt tccagcggga
    23461 tagagagaaa gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg
    23521 tgtttgcgga taattccaaa agagctcgat cagtcccctc gccccttcgg ggagagggtt
    23581 agggtgaggg gaacaggcag caccagtgca aatatttacc ctcaccccgg ccccatagtt
    23641 aacgatgagt cagcttgtcc ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc
    23701 gccggtacct tcttctccta agcggtcgcc cgggttacgc aacgggcaat cactgcgcga
    23761 aaggcagcca caaccaatac atccgtccag ttcgtcacgc agcgccacta aggtatgaat
    23821 gcgccgatcc aactcttctc gccattggga cgaaagctgt ttccactctt tcgcacttaa
    23881 cgtatgccct tctggcaaca cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat
    23941 acgctgagcg attttgataa ttgcgacata tcgcaacaca tcacgtttat atcgccgctg
    24001 attgccgctg ttacggatac tagtaatcaa ccctttgctt tcatagaaat gcagcgccga
    24061 taccgccaca ccgctgcgtt tcgccacttc gccaggggtt agcagcgctt taatgcgggg
    24121 taatttcttt tccataaatc gctttacctc aagttaactt gaggaattat actccccaac
    24181 agatgaatta acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat
    24241 tcaggatctt atcgcatgga ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt
    24301 cgcaaaaaaa tcaggctatt caaagtggta cttgcaacgg atgttccgca cggtgacgca
    24361 tcagacgctt ggcgattaca ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg
    24421 caccaccgag cgtccgattt ttgatatcgc aatggacctg ggttatgtct cgcagcagac
    24481 cttctcccgc gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct
    24541 gtagttttat tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc attgcataaa
    24601 cacctgcggc ggcatcgcct tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt
    24661 gcgcagccag ttaacctgct cctcagtttc gacgccttca gcgatcgttt ttaaccccag
    24721 gctgtgcgcc agctcgatga tgtgttccgc aatcaaatga ctggttttgt gggtggtcag
    24781 cgtttcaaca aacgatttat cgattttcaa aatatcgaca ttcaatgatt taaggttatg
    24841 caagttagag tagccaatac caaaatcatc aattgccact tcgtaaccag cctggcggaa
    24901 agccagaatt aatcggcgtc attttttcga catcaagaaa tgcatgctca gtcacttcaa
    24961 atttaatttg ctgcggacgc accgcgtatt gctctgtttt ctgattgatt cgcgctatca
    25021 accgtgacgt atgaaaatcg gaggccgaca ggttaataga aacatagcga tcggcatgtg
    25081 ttgccaggta cgcgcccaga tcgcggaaga cattatcaat aacataatca gttatctgtt
    25141 ctatcatccc ctccttttct gccagcggaa taaactctgc cggattcatt atttgcccct
    25201 gctcaccagg ccaacgtaac aacgcttcag cgccgataca tttttctgtt ttgatatcga
    25261 ttattggctg gtaataaaga caaagttgat gtttttcgag ggcgcgttgc aatttacgtt
    25321 tgggagataa atagttttgt cgaatacgta gccagagtaa tagcagaaca agactcccca
    25381 aaataccggc gggcaacgca aaaataagat gattataaaa atgggttata agacgttgat
    25441 atgaagtcgc aacaatggct gcaattgggc gttttgtcga ataaattgtc gcatataaat
    25501 agccatttct ttgtacggtt aaatccttca aatgaatcag cggagaaaac gttgctgccg
    25561 aggcctcttt gcttaacgag aaaaaggttt tcatcaccgt atcatacaca ccccattgca
    25621 atgtcgggtc atcagacatc acttcactcc agaagagagg gttgataacc gccacataat
    25681 ttccccgctg catataggtc attttatagc cagagaaaaa aggcgtatcg cggtaataat
    25741 agatagaaac gttaggttca cgcttataat cggccggtgc aatcgtatag ccgtttacag
    25801 gcgctatcag cgatgagcat aaaaaatggt tatcacgaat tcattaatat aaagatagcc
    25861 acgaataata ttcaacattc gcttttgatg ggctggagtg caaacttgcc cctgatagcg
    25921 ttcagctgca tcgctcacta aatctgcctg gcgaatgacc agctcggatt tatccagtgc
    25981 cagttgagca aaggttcgga gctgattatt cacttctgat tttgcccaaa ggaaggcaat
    26041 ccatagcgaa agaatgatgg gaaagagaac taaaaagata atgcccggca acgccagtaa
    26101 ttggtgtcgt gcacgatgac tcattgctat caatttcctg cctgtttata cccggccatt
    26161 gcaaggtgaa accctggcta ccgacgtctt tagataaatc ttacggtata aatagcactt
    26221 ttgcctgatt tgaaattgat acctttttaa tcgtcacctg gatgaaaata attcattgat
    26281 attcaaaatt aattttctac aggaatacag aggcggcggg aagatatgcc cttaatgcca
    26341 ctgaaaaacc acataatttg tacagcaata aaagtcacgg cctaatatgg ctaacaaaca
    26401 atcaattgtg atatagttca caaaattaat gaaacaaaca gagtgtttca tttttgtgtt
    26461 ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc
    26521 agggcatcgg ctcttttcag aggaattgtt tgatggctac cctcaccact ggcgtggttc
    26581 ttcttcgctg gcaacttctt agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc
    26641 gttttcgtcg gtctgattat gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt
    26701 ccgcctgttg gttcgcaatg gggttgcttg gcatcacaat ggcggacatc accgctatct
    26761 ggcacaacat cgagtcggtg atgatagaag agatgaatca gacaccgcca caatggccaa
    26821 tgattttgac ctgatacgta gaa
//
LOCUS       DAJTYL010000052        25825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYL010000052 DAJTYL010000000
VERSION     DAJTYL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.052"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO572_002085"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO572_002085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6661088.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO572_002086"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO572_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6661089.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO572_002087"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO572_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6661090.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO572_002088"
     CDS             3807..3995
                     /locus_tag="OO572_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6661091.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO572_002089"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO572_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6661092.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO572_002090"
     CDS             4756..5115
                     /locus_tag="OO572_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6661093.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO572_002091"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO572_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO572_002092"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO572_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6661094.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO572_002093"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO572_002093"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6661095.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO572_002094"
     CDS             10913..11254
                     /locus_tag="OO572_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6661096.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO572_002095"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO572_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO572_002096"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO572_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6661097.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13681..14967
                     /gene="dctA"
                     /locus_tag="OO572_002097"
     CDS             13681..14967
                     /gene="dctA"
                     /locus_tag="OO572_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6661098.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15188..16684
                     /locus_tag="OO572_002098"
     CDS             15188..16684
                     /locus_tag="OO572_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6661099.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO572_002099"
     CDS             complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO572_002099"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6661100.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17941..18708
                     /gene="pdeH"
                     /locus_tag="OO572_002100"
     CDS             17941..18708
                     /gene="pdeH"
                     /locus_tag="OO572_002100"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6661101.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18778..20838
                     /locus_tag="OO572_002101"
     CDS             18778..20838
                     /locus_tag="OO572_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6661102.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21020..22342)
                     /locus_tag="OO572_002102"
     CDS             complement(21020..22342)
                     /locus_tag="OO572_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6661103.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO572_002103"
     CDS             complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO572_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6661104.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23815..24714)
                     /locus_tag="OO572_002104"
     CDS             complement(23815..24714)
                     /locus_tag="OO572_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6661105.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25234..>25825
                     /locus_tag="OO572_002105"
     CDS             25234..>25825
                     /locus_tag="OO572_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6661106.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6156 a         6487 c         7029 g         6153 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc cccccccccc ataaccttac aagacctgtg gttttactaa aggacaccct
    13681 atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt
    13741 attctccttg gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc
    13801 ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc
    13861 attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac
    13921 tttgaaattg tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag
    13981 cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac
    14041 gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc
    14101 gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt
    14161 ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt gatcgaaagt
    14221 ttctcgcagg tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg
    14281 ctcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg
    14341 cagctgatta tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca
    14401 atcgctaaag cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg
    14461 ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg
    14521 gagaaactcg gctgccgtaa atcggtggtg gggctggtca tcccgacagg ctactcgttt
    14581 aaccttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact
    14641 aacagtcaga tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct
    14701 aaaggggcgg caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg
    14761 gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca
    14821 gaagctcgtg cgctaactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag
    14881 tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat
    14941 ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga
    15001 gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag
    15061 gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt
    15121 aacgtcattt agactgaccg acaacgggca gtttttttat taccaggata gttgatcagg
    15181 ggttcacatg cagggcataa aaattcgact tttagcgggc ggtttgctga tgatggccac
    15241 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    15301 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    15361 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    15421 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    15481 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    15541 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    15601 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    15661 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    15721 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    15781 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    15841 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    15901 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    15961 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    16021 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    16081 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtgacag
    16141 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    16201 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    16261 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    16321 gaaactggag ctgcagaaac tgtttgccgc ctatgcacgg gctgataccg atattctgat
    16381 gggccagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    16441 gaaactgcgg caggatttcc ttaacagcct gactgtggag atgttaaatc aggatctgcg
    16501 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    16561 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcta ccgccgctgc
    16621 gaccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    16681 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    16741 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    16801 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    16861 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    16921 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatcaac
    16981 taacgcttca ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    17041 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    17101 cgcgtcttcg tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgctggta
    17161 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    17221 cacttttccg cctttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    17281 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    17341 ttcgcaaatc gccgcagact gctcgctcgc cagccagaat ttggcggcgg cttcgttacg
    17401 ccagtagtag aacgtacgtt cgccggtgct gtcggtttca atgtagtaaa ggcccggaag
    17461 acggttttcc atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag
    17521 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    17581 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    17641 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    17701 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    17761 ttgctggtca accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    17821 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    17881 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    17941 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    18001 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    18061 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    18121 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    18181 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttgtaga gcacggcctg
    18241 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    18301 ctgcgccaga ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    18361 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt
    18421 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    18481 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag
    18541 cttttacatc taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg
    18601 gaagagtggc gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    18661 cgcccggcac cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    18721 tccgccgggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    18781 agcaaggcag gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    18841 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    18901 gtctctgcgg agttgaatcg tccgttcgct atccgtggca atctgggcgt ggtgtgggag
    18961 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    19021 gacatcattc ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    19081 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    19141 aagctcgaaa agcccgacgc gcgcctgatt cgcctctctg aaaagaacaa taactggacg
    19201 tttaatcttg ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    19261 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    19321 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccattcag cgaagttact
    19381 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acatttttgg cctgaaggcg
    19441 cagggacgtt ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg
    19501 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    19561 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    19621 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    19681 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    19741 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    19801 gtctacacca ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    19861 cggctggcgg acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg
    19921 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    19981 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg
    20041 cggcgcattg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    20101 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    20161 gcgaatattc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    20221 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    20281 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    20341 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    20401 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    20461 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag
    20521 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    20581 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    20641 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    20701 ccgctgattg cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accggcggcg
    20761 gcgttactgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    20821 tcgcagatga agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt
    20881 tagatttttg gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc
    20941 ctacaaatcc ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc
    21001 atcaggcttt tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg
    21061 cgattaacgt caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt
    21121 tagcctgcaa ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt
    21181 tgtaagagaa cgatgctccg gtgtaacgca cttctgtcgg aaacagctct ggtaacagcg
    21241 cacccatcgg cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa
    21301 ccagaatcgg gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga
    21361 tcagcgtggt gatgatcacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg
    21421 cgactggcac catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt
    21481 tacgcggcag gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg
    21541 tcatgatgta aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc
    21601 gaacatgttt ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga
    21661 ctttctcaaa caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca
    21721 gcaccgccga gaagataaat ggcacacgcc agccccagct cataaactgc tcatcggtca
    21781 gtagccagga aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccta
    21841 gctgtgggaa ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca
    21901 gcgccgcgcc gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca
    21961 gcagcagtgg ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg
    22021 tcgaaatccc catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc
    22081 caaaatgacc aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg
    22141 cgagcgactg tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca
    22201 caataacggc cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga
    22261 gagaggcgac aaggacttta ttacgcgagt tgatcggcgt gtattcttgt tcgtggtcga
    22321 gtgttgtggc tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt
    22381 acgcacagca aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg
    22441 caaaaagggg ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt
    22501 tataaatagt ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg
    22561 ctggggttat gagttggagt gaaattgttt gctcccaaat agatggctga agaggctctg
    22621 gggaagagtt tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta
    22681 tccggcctac ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc
    22741 atccggccta ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc
    22801 ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat
    22861 cagccccagc acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt
    22921 gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat
    22981 cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag
    23041 atagttggcg aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc
    23101 aatgctgttc aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt
    23161 gatcgaaagc gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag
    23221 atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg
    23281 ggcacgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc
    23341 caggccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa
    23401 cgtggcggct agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag
    23461 caacatcgga tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg
    23521 gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc
    23581 gttaaagcgt tctgtcgcgc gaataaggtg cgcaataacc ggttggcgtt ggactttttc
    23641 ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc
    23701 caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg
    23761 cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc
    23821 caggtgctct ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag
    23881 ctcccgacgc tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa
    23941 cacttcaata agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat
    24001 aatccccaga ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat
    24061 gccgccagac ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg
    24121 tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg
    24181 cggataacca aagcgcgtca gatagtgcgg actggcacag ttgaccatgg ttagtttgcc
    24241 gaggggacgg gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa
    24301 accatcatga agaatatcta ccggacggtt atggctactc agttccagct caattcccgg
    24361 atgctgatag agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg
    24421 gatgtcgatg cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag
    24481 ttcgctgaga ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt
    24541 gagtttgacc cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc
    24601 ctgtatctgg cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa
    24661 actttccagc tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt
    24721 gaccgattgt tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc
    24781 taacatctaa tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg
    24841 aaaagggcgg aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag
    24901 tgattccctt taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt
    24961 gccatttgtt attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac
    25021 caccaatata aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag
    25081 gcgtttagct gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg
    25141 gccaccgcca aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa
    25201 gtctttttaa cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag
    25261 tcaatattta ttcatggaat aaaaatcagt ttacagcagc gtattccagg agtgagtatt
    25321 cagggggtca gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta
    25381 gttatgctcg atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg
    25441 gtgcagtttc ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta
    25501 caggaagtta tccattttaa tgtgctggcc attgtgcctc gcgattcaac cgtcgagaca
    25561 tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg
    25621 acgacgccgg aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt
    25681 ttaaccatgt tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt
    25741 accggaacgg taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc
    25801 acccaggcgg caatgatgtt aaata
//
LOCUS       DAJTYL010000053        25749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYL010000053 DAJTYL010000000
VERSION     DAJTYL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.053"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            214..507
                     /locus_tag="OO572_002106"
     CDS             214..507
                     /locus_tag="OO572_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6661107.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            579..1238
                     /locus_tag="OO572_002107"
     CDS             579..1238
                     /locus_tag="OO572_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6661108.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1330..1776
                     /locus_tag="OO572_002108"
     CDS             1330..1776
                     /locus_tag="OO572_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6661109.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1983..2883)
                     /gene="hlyE"
                     /locus_tag="OO572_002109"
                     /pseudo
     CDS             complement(1983..2883)
                     /gene="hlyE"
                     /locus_tag="OO572_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3256..3675
                     /locus_tag="OO572_002110"
     CDS             3256..3675
                     /locus_tag="OO572_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6661110.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3675..4943
                     /gene="umuC"
                     /locus_tag="OO572_002111"
     CDS             3675..4943
                     /gene="umuC"
                     /locus_tag="OO572_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6661111.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(4989..5519)
                     /gene="dsbB"
                     /locus_tag="OO572_002112"
     CDS             complement(4989..5519)
                     /gene="dsbB"
                     /locus_tag="OO572_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6661112.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5665..7206)
                     /gene="nhaB"
                     /locus_tag="OO572_002113"
     CDS             complement(5665..7206)
                     /gene="nhaB"
                     /locus_tag="OO572_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6661113.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7427..8146
                     /gene="fadR"
                     /locus_tag="OO572_002114"
     CDS             7427..8146
                     /gene="fadR"
                     /locus_tag="OO572_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6661114.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8198..9730)
                     /locus_tag="OO572_002115"
     CDS             complement(8198..9730)
                     /locus_tag="OO572_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6661115.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10060..11358
                     /gene="dadA"
                     /locus_tag="OO572_002116"
     CDS             10060..11358
                     /gene="dadA"
                     /locus_tag="OO572_002116"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6661116.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11368..12438
                     /gene="dadX"
                     /locus_tag="OO572_002117"
     CDS             11368..12438
                     /gene="dadX"
                     /locus_tag="OO572_002117"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6661117.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12606..14020)
                     /locus_tag="OO572_002118"
                     /pseudo
     CDS             complement(12606..14020)
                     /locus_tag="OO572_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14631..16367)
                     /locus_tag="OO572_002119"
     CDS             complement(14631..16367)
                     /locus_tag="OO572_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6661118.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16462..17376)
                     /gene="ldcA"
                     /locus_tag="OO572_002120"
     CDS             complement(16462..17376)
                     /gene="ldcA"
                     /locus_tag="OO572_002120"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6661119.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17476..18087
                     /gene="emtA"
                     /locus_tag="OO572_002121"
     CDS             17476..18087
                     /gene="emtA"
                     /locus_tag="OO572_002121"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6661120.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18089..18823)
                     /locus_tag="OO572_002122"
     CDS             complement(18089..18823)
                     /locus_tag="OO572_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6661121.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19024..19278
                     /locus_tag="OO572_002123"
     CDS             19024..19278
                     /locus_tag="OO572_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6661122.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19328..20788)
                     /locus_tag="OO572_002124"
     CDS             complement(19328..20788)
                     /locus_tag="OO572_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6661123.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20725..21282)
                     /locus_tag="OO572_002125"
     CDS             complement(20725..21282)
                     /locus_tag="OO572_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6661124.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21308..22162)
                     /locus_tag="OO572_002126"
     CDS             complement(21308..22162)
                     /locus_tag="OO572_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6661125.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22162..22971)
                     /locus_tag="OO572_002127"
     CDS             complement(22162..22971)
                     /locus_tag="OO572_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6661126.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(22981..23739)
                     /locus_tag="OO572_002128"
     CDS             complement(22981..23739)
                     /locus_tag="OO572_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6661127.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23736..24716)
                     /locus_tag="OO572_002129"
     CDS             complement(23736..24716)
                     /locus_tag="OO572_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6661128.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24716..25738)
                     /locus_tag="OO572_002130"
     CDS             complement(24716..25738)
                     /locus_tag="OO572_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6661129.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6415 a         6339 c         6694 g         6301 t
ORIGIN      
        1 atgaactgtt ataatataac aatcccttac gggagaattc cagtggcttt tctgcgttcg
       61 gtaaaatccc ggcgcggcat tttttgccgg ggggattact tataaagggg catggactca
      121 acaaaccgca gaaatctaca gctggctggc ttgtggcgcg ggtttcattg atgttagctt
      181 atgccaaaac ctggcatctt aaagagtaaa agtatgtttt gtgcgattta tcgaagcagc
      241 aagcgtgacc agacctattt atatgtcgaa aaaaaagacg atttttcgcg tgttcctgag
      301 gaactgatga aaggttttgg tcagcctcag ttagcgatga ttctgccgtt ggatgggcgg
      361 aagaaactgg tcaatgctga tattgaaaag gttaaactgg cattaaccga gcaaggttac
      421 tatttgcagt taccgccacc acccgaagat ttgctgaagc aacatctttc cgtcatgggg
      481 cagaaaacag acgacactaa caaataaccg atatccggcg gtggcattat ctttgtcggc
      541 gcgggttttc atatccacga taaggtgagg ggaacgttat gtatcaacat cacaactggc
      601 aaggtgcgct gctggattat ccggtgagta aagtagtctg tgttggcagt aactatgcca
      661 aacatattaa agagatgggc agcgcagtgc ccgaagagcc agtgctgttt attaaaccag
      721 aaacggcact gtgcgatctg cggcagccat tggcgatccc atccgatttc ggttcagttc
      781 atcatgaagt cgaactggcg gtgttgattg gcgcgacgct gcgtcaggca acggaagagc
      841 atgtccgcaa agccattgcc ggttacggcg tggcgcttga tctgacattg cgtgatgttc
      901 agggaaaaat gaagaaagcc gggcagccgt gggaaaaggc taaagcgttt gataactctt
      961 gtccgctttc cgggtttatc cccgcggcgg aatttaccgg cgatccacaa aatacaacgc
     1021 tgggcctgag cgtaaacggc gaaaaacgcc agcaaggtac gactgcggac atgatccata
     1081 aaatcgttcc gttgatcgct tatatgagca agttttttac cctcaaggcc ggtgacgttg
     1141 tgttgacagg cacgcctgat ggcgtcggcc cgttgcaaag cggtgatgag ctgacagtca
     1201 ctttcgatgg gcattctttg acaactcgcg ttttgtaata ctttttgccg ccagaaagcg
     1261 gcggcaaaac ttgcatcgct gtgccagact ggttataagg tgcgttttaa cgtaatggcg
     1321 gaacacctga tgagcgatgt acctttctgg caaagtaaaa ccctggacga aatgagcgat
     1381 gcggaatggg agtcgttgtg tgatggttgc ggtcagtgtt gcttgcataa actgatggat
     1441 gaagacaccg acgaaatcta cttcactaac gtcgcctgtc gccagctcaa tattaaaacc
     1501 tgtcaatgtc ggaactacga acgtcgtttc gagtttgaac ccgactgcat taaattaacc
     1561 cgtgaaaatc tgccaacatt cgaatggctg ccaatgacct gcgcttatcg gttgctggcg
     1621 gaaggtaaag atttacctgc gtggcatccg ctacttactg gttcgaaagc ggcaatgcat
     1681 ggtgaacgta tctctgtgcg tcatatcgca gtgaaagaat cagaagtgat tgactggcag
     1741 gatcatatct taaataaacc tgactgggcg cagtgataaa tttaatagat gctgatgggg
     1801 gctttcttaa tatacgtctg gatgctcgcc atttcgtagc cctgtttttg aaagcgctct
     1861 gtcataatga gagttacccg ataccttatt atcaacaggt aactctcaat ctttaaaaaa
     1921 cgtctgtgtt tttaaatgtg ataaacctta taccttgagt acatggagag aataatcgct
     1981 tatcagactt caggtacttc aaagagtgtc tttttaccgt gtcttttctg atactcatta
     2041 caggtgttaa tcattttttt ggccgctgct tttagcaaag aaagcattaa atcatcataa
     2101 tcaacataga atctggttgt ttcagtttcc gtttttatct ccccgatggc ggctatttcg
     2161 gtggttaatt tcaatttggc ggcatcgata tctttattcg cttgtttaac cgtgttagac
     2221 agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg aatcagtttc
     2281 ccttcaacta cgcccgcagc aatagaatag gaaatgatta aaccaaatgg accggcgacg
     2341 acatcggctg cggcaccggc atacgcttcc ttcctgattt tatctacctg tgactggaaa
     2401 tagctgcttt tttctgaaaa atcattggtt aactggctat ctaacgccag cagtttcccg
     2461 gaagcgttgt tgaaactttg tgagcttacc agcagggatt tttgcgcttc attcagcttc
     2521 gtgatgccgt catccagtac cttaatgagg ggcggatgct ttcttctcat tgtactcatc
     2581 aaatagcaaa atatacgctg cgagcaattg cgtcgcaaca ccacaccatt catacactgt
     2641 ttgggttgct tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac
     2701 taaaacggag gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc
     2761 atcaaaggtc tgccagggga tgacctgatc gagatattta ttataaagat ctaatgctcc
     2821 atctgcggtt tcgattgcgt ttttaactac ttctaccgtt ttatctgcaa cgatttcagt
     2881 cataatcatt cgcctcttta aatatataaa ttgtaatgaa actcctgttt tacaactatt
     2941 aataaatttt acttcatcta attcatagtt agccgggcgg gatgcgtcaa tgtctttatt
     3001 tctattaata tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc
     3061 tatttttttg ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc
     3121 acgtgagcac aggataagag aacgaaaaat cagcagtcta tgcagcgaca aatattgata
     3181 gcctgaatca gtattgatct gctggcaaga acagaccact gtatataaaa acagtataac
     3241 ttcaggcaga ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt
     3301 ccgctattta gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgttgaa
     3361 cagcgcatcg atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa
     3421 gcaagtggtg attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat
     3481 agtgctatta ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg
     3541 gtgaaaaaat tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg
     3601 cccattacca tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt
     3661 aaggcgatgc gctgatgttt gccctctgtg atgtaaacgc gttttatgcc agctgtgaga
     3721 cggtgtttcg ccctgattta tggggtaaac cggtggttgt gctatcgaat aatgacggtt
     3781 gcgttatcgc ccgaaacgct gaggcaaagg cgctgggcgt taaaatgggc gatccctggt
     3841 tcaaacaaaa agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc
     3901 tttacgcaga catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg
     3961 agatttacag tattgatgaa gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc
     4021 tgactgattt tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg
     4081 gtgtggggat cgcccagacc aaaacgttgg ctaagcttgc caatcatgcg gcaaaaaaat
     4141 ggcagcggca gacgggtggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     4201 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     4261 acgcgatggg gatcaaaacc gttctcgatt tggcggatac agatatccgg tttatccgta
     4321 aacattttaa tgtcatgctc gaaagaacgg tgcgtgaact gcgcggcgaa ccctgtttgc
     4381 aactggaaga gtttgcaccg acgaagcagg aaattatctg ttcccgctcg tttggtgaac
     4441 gcatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     4501 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     4561 catttgcgct caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca
     4621 ctcaggacag cagggatatc attaacgctg ctacgcgatc tctggatgcc atctggcaag
     4681 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     4741 cgcagctcaa tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatggcgg
     4801 taatggatac tctgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     4861 tccaacaaca atggcagatg aagcgagcta tgctttcacc acgttataca acgcgaagtt
     4921 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagcgctccc gcaggagcgc
     4981 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcacc
     5041 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     5101 atacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     5161 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaat
     5221 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     5281 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     5341 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     5401 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     5461 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     5521 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     5581 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     5641 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     5701 ttcggttaca ggggcaagcg taaactcgac gcagagcaag ccgacgagtg tcagtacgag
     5761 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     5821 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     5881 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     5941 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     6001 aatggatgac agcagaccgt tgaaaatata gaacagcgac agctgagcat gttcagatgc
     6061 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     6121 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     6181 agcatgttca tcggtgacac cggtcaatga tgttgccaga ataatgactg acaaaccaat
     6241 caagccaact tccgccagat gcaacgccag cgcagtcacc agccagacgc caataatcgc
     6301 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     6361 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     6421 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     6481 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     6541 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     6601 caggctacgc agaaagccac ggaactgttc cagaaccacg ttgtaatgct tgtcgatatg
     6661 actatcgtct tgcaggtcgg tgtcttcggt acgggaagag gctacgcgat gataaatacc
     6721 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     6781 ggagaggaac gctgccgcca cgcaaaaaga gagcgacagc agcattttgg agcgaatgct
     6841 taacagcaaa cgggtaaata tgaacagcaa cagctgtttc ataaaataga tacccgccac
     6901 cataaacatc aataacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc
     6961 cgcgctggtc atgccgatga ataccgcttc gatagccaac agaccaccgg ggagtagcgg
     7021 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagcccgc
     7081 gacgaaaggg ctgatgagga aaattaacgg gtttacgatt aagaaaatga tgagggcgag
     7141 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     7201 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa
     7261 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     7321 aaatgaagcc ttgatccctt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg
     7381 gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc
     7441 gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt
     7501 ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg
     7561 tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca
     7621 tggcaagccg acgaaggtga ataatttctg ggaaacttcc ggtttaaata tccttgaaac
     7681 actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg
     7741 taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca
     7801 ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga
     7861 ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct
     7921 taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc
     7981 gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca
     8041 cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg
     8101 gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa
     8161 agagcaaccc cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg
     8221 ggcaacgttc cagcaactcg atgctgccgt cttcgttttg ctgttcgagc atcacatcaa
     8281 atccccacag gcgatgcaca tgcttcagga cttctttgcg cccccgatcc agcggtgcgc
     8341 gattatgtgg aatataacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga
     8401 tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct
     8461 cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcatcat
     8521 ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa
     8581 aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg
     8641 cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat
     8701 cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat
     8761 aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg
     8821 ttactttccc ttcatcatac agatggttaa ggatggtata gtgccagaag gtcgcacagc
     8881 cttcgttcat cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa
     8941 tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga
     9001 gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct
     9061 cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac
     9121 ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca
     9181 cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca tcaacgccat
     9241 aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac
     9301 tggcgtcggt ccaactgcgg aataagcaat tgtttttaaa gaaagagtta tgtccatagc
     9361 aggcgtgtgc catcaccagc gcctgcatag taatggtgtt ctcttccatc aagtaagcga
     9421 tacacgggtt agagttaatg acgatttcat aggccagccc ttgctgaccg tgcttataca
     9481 gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc
     9541 cgacgctgga ataggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt
     9601 aggtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca
     9661 ggtcgaacgt ccagtcgggt ccatcgctca aacgtgtggt gtccttattc atagaatcga
     9721 tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca
     9781 agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta
     9841 ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt
     9901 cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg agtcagctca
     9961 ccataaaaaa gccgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat
    10021 tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga
    10081 agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc
    10141 gtcattgatc gggagccggg ggccgccctg gaaaccagtg ccgcgaatgc cgggcaaatc
    10201 tcccccggat atgctgcacc gtgggcagca ccaggtgtgc ctttaaaagc gattaaatgg
    10261 atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa
    10321 tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg
    10381 atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat
    10441 attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat
    10501 gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg
    10561 gaatccagcc gcctggcgga agtggatccc gcgctggcag aagtagcgca caaactgacg
    10621 ggcggcctgc agttacccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg
    10681 gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg
    10741 ctttgcgacg gcgagcaaat ctacggcgtg aagtttggcg atgaagtgat taaggccgat
    10801 gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt
    10861 ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg
    10921 ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc
    10981 attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt
    11041 cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag
    11101 gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggagt
    11161 acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct
    11221 tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat
    11281 gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta
    11341 catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg
    11401 caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc gcgcgtctgg
    11461 tcggtggtaa aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg
    11521 gccaccgatg gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc
    11581 tggaaggggc cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat
    11641 gaccagcacc gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat
    11701 gcgcggctaa aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg
    11761 ggcttccagt ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt
    11821 ggcgaaatga ccctgatgtc gcattttgct gaagcggaac atcctgatgg aatttccggc
    11881 gcgatggcgc gtattgagca ggcggcggag gggctggagt gtcggcgttc gttgtccaat
    11941 tcggcggcga ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt
    12001 ttgtatggcg cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg
    12061 gtgatgacgc taagcagtga gattattggt gtccagacgc taaaagcggg cgagcgtgtg
    12121 ggctacggcg gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcagga
    12181 tacgccgacg gttatccgcg ccatgcgcct accggtgctc ctgttttagt ggacggcgtg
    12241 cgcaccatga cggtggggac cgtctcgatg gatatgctgg cggttgattt aacgccttgc
    12301 ccgcaggcgg ggattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat
    12361 gtcgccgccg ctgccggaac ggtgggctat gagttgatgt gcgcactggc gttacgcgtc
    12421 ccggttgtga cagtgtaact tgttgtaagc cggatcggag gcagcgtctt ctggttgcga
    12481 aaaaatcatc catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc
    12541 atgatagtgt ccattaaaag aatggacact atttccccgg aacctgaact cgccgcacag
    12601 gcgttctaca taaaacgctt acgcttcatt gttgactcgg agtcgacttc agataaatca
    12661 cgcttcaccc ttgatgcaga ttcgggttca tgttcagatt gttgattaac aggctgcgag
    12721 tcctgttgtc tttccagact caacaggccc aactgttgat ccaggagctc caggtcctcc
    12781 tgtaccttca cgcgctccag gagttccggg tcatccagga actccagatc cataaggtcc
    12841 tccagctcct cgagtccttc caggtcaacc tagccttcct tgtgcaaaaa gtccaaaagg
    12901 tccttcaggc cctgctgttt tttcaggccc tcgacggctt tgagcagcgt cggtaaattg
    12961 tctttgtccc acgggttacc attacagtca aacaccttca ggctctgggg ctgcggcacc
    13021 tgtaccgttt taaacttgtt ttctccacag tagtaaatta ttagtttggg cggcagccgt
    13081 ggtgaagaga tcaaatcgtt actagaacag tcaaatactt tcaggcgagt caagttctcc
    13141 agcatctcat caggtattga gttaatattg ttgccggcac aggatagttt atacaggtac
    13201 tggggcatcg acggcggtaa aaattgcaag aagttttcag aacagttaat cactctcagg
    13261 ttagtcgaca gctcaggcag ccagataata ctgttttcat agcacttcag taccatcagt
    13321 tttgagggct gcaccttcgg taacaagatc aagacgttat ctgtacagtc aagcaccagc
    13381 agccactttg gcatcacctt cggtaacgcg gtcaacacgt ttttactgca gtaaaattca
    13441 atcagatgtg gaggcaaatt ctcagggagt gaagtcaatc ccaggttgct aagattgagc
    13501 atactccgtc ttgtaatgag acattccttc attctgcgca atgcttctgc ccggttttca
    13561 atccggcttg ctggcaggtt ttcttccagg atattttcct tgatttcttc cagactttct
    13621 ttcaggtctt cttggggaat ttctgactgt ttgttttgct ggttttcttc caggatttcg
    13681 tacaggtttt tttgcaggtt ttcttgcaaa ccatcctgct gtatagccca ttcgcaccat
    13741 ttggcgacca tcatgaactc gttatgttgc tgattcagac gagtccccaa cgtgatgatg
    13801 gtattgttgt ccacctgtat gttcactttg cccgcgatac tgaggtcaat gatctgccag
    13861 ggggcatcac cccagctgaa tttttgcggg ctgagatact tcatgttctg caaaatttga
    13921 ttcttgagaa gagccgccct ggcgggcgtc gtacgcctgg gttaacggaa gatgggggaa
    13981 tggcgggggc gaatgggcag atgtctttaa agctagccat aatgctacgt ttccttgaaa
    14041 taaatatcct ctggcagcgc cggagacgtg aatgtggtca ccgcaaagga gccacaggac
    14101 gtcatttgcg ctgatatcac tactcatcgt tgactttttt cctggtcaga aatgccgtgt
    14161 cgggtctggt acatgctgta ttacggccta ggtagctctg acgtattaaa gaaaatacga
    14221 gagtgaacga cccggaaata atcgttatta agcaaaggaa taactataag aaatcgctca
    14281 tgcatccggt gctacaggcg gatgaaacgc ctattgtgtg ggctatgtca gtgcggcagg
    14341 ttgcgtgggg gaatatgttc aggcgatgtc aggcaggggc ccctgatggt tgaccggtaa
    14401 tgcggggctg gtttttatta cctgttacag tcggaaaaag cccttacgct gactttcttg
    14461 agccggtgtt ttgagttcat tagccagagc ctgcaatggt ttgtcatcca taatttaacc
    14521 ttcttctgat gttgggatga gcatatcaaa atcaggaaat gacaagttta ttacaggctc
    14581 aggctgcgtt cagtaaagca tcgcatccgg cgatagatgc tgcattaaat ttaagattca
    14641 gcttcctctt cagccacccg aacgccaatc ttcaacactt cattatcttc tttctcggcc
    14701 accgtccaga tcatcccggc aaactctacc tggtcaccga caaccggtgc cgcgcctagc
    14761 aactgctgga caatttcacc cagcgtttgc tgtttatcac gatattctcg cccgtcttcc
    14821 aggccatata tcagcgcaac atcagcatat ttggcgctgg cttcgagaat gaagtcacca
    14881 aagaagcgtt gatccagcgc gaccggtggc gactggctga acagtttacc gagcgccggg
    14941 agatcgcgtt cccgaccaat tacacacaat acatcgcctt cgcgcagtcg ggtgctgccg
    15001 gtaggatgaa gcaactggtt atcacgaaac agggctgcaa tacgcgtctc ttttggcata
    15061 tgcaaatcgc gcagtgccgc acctacacac catttatcag cactcaattg ataaacaaac
    15121 tgctcccacg gattttccgg atgaatatcc aggccaacgc gtgacaccgg acgtccgacg
    15181 ggcggaacca ccactttggc ttttttcgcc gcccacgaga gtgatgttcc ctgcaagagc
    15241 agtgaaacca gaaccacaaa gaaggcgaca ttaaagaaca gtcgcgcatt ctccagcccc
    15301 gccatcatcg ggaacacggc taggatgatt ggcaccgcgc cgcgtaatcc cacccagctg
    15361 ataaacacgc gctcacgcag attgaaaccg cggaaaggga gcaatccagc aaataccgaa
    15421 aggggacggg cgaagaatat catccatgcc gacaaaatga gcgccggaat ggcaataggc
    15481 agcagatcgc ttgggttaac caatagcccc agcaccagga acatggcgat ttgcgccagc
    15541 caggcgaggc cgtcgaaatt ttgcaggatg ccgtagcggt tgcgaatcgg ccgattaccc
    15601 agcagaaaac cgcacagata aacagccaga ataccgctgc cttccagcgc cgtggttaaa
    15661 gcaaaaatta aaataccgcc gcttaatgcc agcaatggat ataatccggc aggcagggcg
    15721 atgcgattaa tcatttgcag cagtaaataa ccaccgccaa gaccgatgac aatcccgagg
    15781 ccaaattgct gcagaatatc gaccacgaac atccaactga cactgctttc atgctgctgg
    15841 atcattgcaa tcagggtaat cgtcagaaag accgccattg gatcattact gccggattca
    15901 atttccagcg tcgagccaac acgttcgtta agccccttac cacccagcag agaaaagacc
    15961 gctgcagcat cggtggagcc gacgatagcg ccgattaata agccttcaat caaatcaaga
    16021 ttaaacagcc acgccgccat catgccggtt aaaccagagg tgataagtac gcccagcgtc
    16081 gccagcgaca gcgccggtcc taacgccaca cgaaaggaac tggcctgagt gcgcatcccg
    16141 ccgtcgagca aaataatcgc cagcgccagg ttacttacca tgtaggcgaa ggggtaatta
    16201 tcaaacggga taccgccgac gccatcgact cccgccagca tgccgatcgc cagaaaaata
    16261 accagaatag gaatgccaag acgggaagaa aatgaactaa gtaatatact gctggtgaca
    16321 aggatggatc ctaagatgaa aaggctaatt attgttgtgg catccaatgg tcggttactc
    16381 ctgattacgc tgtctcttat ataaacccta ccatattagc ggcagagaca gcgttttact
    16441 tagccctgaa gcgtgatttt tttacatttt aagaacagga tgaccgctaa tcgtcaactg
    16501 agcgccttcc cgggtgttat tcagaatggc atgtgcgccc aacggcagcg taaccgtgcg
    16561 ttgttcatga ccaaaatcga ggccggtaat gagtggaatc gacaggcggg aacgcaaaaa
    16621 cgcgtacact gactccaggt tgtaacctgc gtcataatca ttgggcgtgc tgccgctaaa
    16681 gctaccgaga ataatcgcct tctgacgtgg caaaattccc gcatgataga gctgcaacag
    16741 catacgttcg acgcggaaag ggtgcacgtt aatatcttcc agcaccagaa taccgttctc
    16801 aatttttggc atccacggtg taccaatcag tgaaatcagc atcgcaagat tgcctcccca
    16861 caacgtgcct tcggtctgac atgtcgggcc ttccccttgc cattcaatgg tgaaggtttc
    16921 attgcgtaac gccagccaga aatggtgctc ggtaaaggcg ttcagttcat ccgcaccaaa
    16981 gtttgctacc agcatggggc cgctaaaggt gatgacattg ccttgcgcca gaagaccgca
    17041 ctgaatggcg gtaaaatcgc tatgtccgca aatgagtaac gggtcatgtt gttggcgagc
    17101 caccagtgcc tgccagtcaa tatctgccag taaacgactg gcaccgtaac cgccgcgtac
    17161 agccagcacg atggtgttgg gggtagtcag tctggcaagg gaattgagat cttccagacg
    17221 ctccgtttcc gtacccgcaa aacgctcaca acgacgggca ataacctcga cgttattgac
    17281 ttgatgcccc gcgtcggtaa ggcgctggat accacgcagc gcggcgtgct gtttaatgct
    17341 gtaacccgat ggggcaatta agtgaaacag agacatggca attccttgct gacaacagaa
    17401 acgaaatgta tatcatgccg cttaggtgtg ccgttgtcac ctcaacggcg attccaggct
    17461 ataaggatag aagaagtgaa attgagatgg tttgcctttt tgattgtgtt attagcgggt
    17521 tgttcatcaa agcatgacta tacgaacccg ccgtggaacg cgaaagttcc ggtgcaacgt
    17581 gcgatgcagt ggatgccaat aagccagaaa gccggtgcag cctggggcgt cgatccacaa
    17641 ttgatcacgg cgattatcgc tatcgaatcg ggtggtaatc cgaacgcggt gagtaaatcg
    17701 aatgccattg gtttgatgca gttaaaagct tcaacctccg gacgtgatgt ttatcgccgt
    17761 atgggctgga gtggtgagcc gacgaccagc gagctgaaaa atccagagcg taatatttca
    17821 atgggggcgg cttacctgaa tattctggaa accggcccgc tggcaggcat tgaagatccg
    17881 aaggtactgc aatatgcgct ggtggtgtca tacgctaacg gggcaggtgc gctgctacgg
    17941 actttctcgt cagatcggaa aaaggcgatc agcaaaatca acgatttgga tgctgacgag
    18001 ttcctcgatc acgtagcgcg aaatcatcct gcgccgcagg ctccgcgcta tatctacaaa
    18061 cttgagcagg cactggacgc gatgtaaatc agtcgcgcac tttgtccgct ttttcccggg
    18121 cttctcgctc gagagagaaa ataatccgct gtaattgccg ctccaccgtc gggctgacgt
    18181 taagaaaacg gaagctcaga cggggagtgg tgatggtttc attcttgcca tcaatcactt
    18241 tgcgctcgct ggtggagatt aactgggcgt caaagtgaaa aacaccccat tgccccatgt
    18301 tgacttcaat ttgagcgaag cgcatacctt cttgtaattc ggcaggcttt gctgtttcca
    18361 gtaatgcgcc catgccgcct aacgacaaat catacaagcg gaaacgtaac gtactgttat
    18421 ccgccagttt ggtctggcaa aaataaggcg gatggagtgg ggcggagatg cggaaatatc
    18481 ggcgtcgttg tacaaaccat aaggtgggag gcggtacggt aataaatgcc ggaagctgca
    18541 agtattcact ctgctgtagt tgttcaacag taaactcgac tttcgcaccc tgagtttcgg
    18601 cggtaatggt aatgtgctgt gcctttagca cggcgatgtt gtcttcggct tgactgccga
    18661 aatccagcac cagtttatcc ggggttattg ccagtatttt gctgatcagc tgcccgccat
    18721 tccaactgag acgcaaagga attgcggctt tgtgcaaatc gcgtaacacg cccaggacgg
    18781 ctaacggatt ttgtttcagg aactgctcat ggtaatgact cacgcggaaa aactcctgac
    18841 tgacaaactg tctttggttt atcggtcata gttaacaaaa cttaatacaa atgcgtgaat
    18901 attttttaca tgttgttctt aaatcagccg acaaacgccc agcatcgatc ccgcccttgc
    18961 ctatacttag agcgttgacg taagcatttc ttgcgaccga ttcatcgaaa cgagggcctg
    19021 aacatgggaa ttattgcctg gattattttt ggcctgatag ccggcattat cggcaagcta
    19081 atcatgccgg ggcgtgatgg tggtggattt ttcctgacct gtattctcgg gatagtcggt
    19141 gcggtggtcg gcggctggct ggcgaccatg tttggcattg acggctccat cagcggtttt
    19201 aatttgcaca gcttcctggt ggcggtggtg ggcgctatcc tggtgctggg cgtattccgc
    19261 ctcctgcgaa gagaataaga ttttcataag gcggatagcg atacagatgc cgctatccgc
    19321 tttcacatca gaacgtatac tcgacacctg cccagaaatt gcgaccttct tcaataaacc
    19381 cttcactgta ggcatattgg gtatcaaaca ggttattgac cgacgcattg acgctgaagc
    19441 catgacctaa ggtgtaatcg gctcgaatgt gggtcaccgc aaaaccggcg gctttttgtg
    19501 aaccgtcact gttgctgtag ctggaggaac gcgcctcttc cgacagcgtt acgcttaacg
    19561 gctcccatgg tttgagtgtc atccatgcgg tcattgtctg cgttggaaga tcggttatct
    19621 tgccgatgtc tttgcgtttg gcgtcagcgt ggatcagagc gtagctcaat cctacatcca
    19681 gtatattgct gattttcccc ttaataccgg catccagacc gctgtaatcc accgtgccgc
    19741 tgttctgatt ttgaatggta tcggcattga tattgtgcga gaggatggca tcactcaccc
    19801 ggttatagta aacgctgacc tcaaatcccc agtcgcgcgt gaaggcacca ttccaggtta
    19861 aatccacccc gcgagcgcgt tccggcttga gctgcgggtt aacaatcgct atctggttat
    19921 acgcaggttt ggatgtggta tagcgttctt tcagcgtcgg aaagcgtgtg cggtcatagt
    19981 acgaaagcgc cagagtgtct tcattggcaa agtggtattt ccccatcacc tgccagttaa
    20041 aagctgactg attgttgtcg tcataatggg tgatgctgcc atctttctcg tgttttttcg
    20101 cttctacgct atcgcgccag tcatagctga ttcccgccac gacatcgaca ttatcagcag
    20161 cagcccattg atattcactg gcgagcgacc aggtacgatc ttcatagcga tcgtaagcgg
    20221 cgtgcggcgc acctttttcc cggtgtacgt catctttcca gttaacggca aacgacagca
    20281 gatcgttttc acgcacatcg gctgccagtt gtaatccggc accgtcgctg taatcggaat
    20341 aatggctgta gctgcctttt ttatttttca aatcagccag cgagttgtac atcatcagcg
    20401 tattttcaaa ggtgtcgcga tacagccgac ttttcagggt aaaacggtcg tttagttggg
    20461 tcgctccctg ataataaaaa ctttctttgt catactctgg ccactgccag tagcgtgatt
    20521 tttgaccaca atttccgctg tatggcgggt tatctttttc accatcctgc ttaatgtaag
    20581 tcagcgtgta ttcatcgttt tcgcgcggtg taaaaccgag cttcacaatg ccgcgtttat
    20641 catcagccga tgaattaatc atcttgccgt gtttgcctgc aatatcatta tttacaccat
    20701 gcggcaggcc gagaaaatcc tgctttagct ggctaccgct gacttgcaaa tacccaagat
    20761 cgctgctggc ggcaaatgaa gcatgcatat cgtaggcatt gtcctgacta cggctccatc
    20821 cctggcgata tcccagactt gcttcttcag ggtggtgata ttaatggctc cgcccatctg
    20881 attaggccca tgaagcagcg acgaataccc tttggaaact tcgactgccc ccagattgtt
    20941 ggtcagaatc cgcgccagat cgaggttgcc gtcatagggg acataaatgg gcacaccgtc
    21001 gaaatagacc ggcacctgcc gactatcaaa gccacgaact tttacctgtt cttcgttgcg
    21061 gctgcctgac ttttgcagca ccacgccggg gacgacactt aatgcctgag cgacattctg
    21121 tttatccaag gccttcatgg tgggttgatc gagaacggtg gtcgtcgtcg atgataccgg
    21181 actggaccat acggtcagcg tgtcgctctc ctgagccgct acggcctgct gggtaaaggc
    21241 aagtgataat gctgtgcaga gataacgttt tttgagtctc atagaataaa ttccgtttaa
    21301 caaagaatta aatagaggct gtgggttgca gactgacttt aatgtcagct ggtgccgcgt
    21361 aataaggcgc ggaggtaata aaaaggcgaa tgccgcagtc gaggtaattt ttgagtgtcg
    21421 tcagattaat tccgccggtc agagccagcg tgcagtgagg agccagcgac ggcgctattt
    21481 gcgcaatttc agttgcctgt tgcggactaa atttgtcgag ctgaagtacg tctggttgtg
    21541 cgcgtaacgc ggcgatagcc tctttcggcg tgtcggcttc aacaacaatt ttcttctcgg
    21601 gtgcgtggcg acgtaactga gcgattgcgc ctgaccagcc ctgattgtca tgaagaaaat
    21661 ggcggtggtt ggcaaacaat aatatggttt ccgcacatcc ggcacgatga attagtcctc
    21721 cggcagccag aattgcctgc gaagccagta agcgggtgcc cggaattgct tttcgggtgc
    21781 aggcgatatt gccgtcagga taacgttcac gaagcaatgc cagcatttga gcgagataat
    21841 cagaaacacc gcaactccac tccagcacat tctggactgc cttccatccc tgatgaagtg
    21901 ctgcggcatt accctgcgca cggattagcc gttgacccgc gttcgcttgt gaaccgtcgc
    21961 tgaccgcgtc atcaatagtt aatcccagcg tagttaacat cttacacgcg acagaaacgc
    22021 cgctgacgca gccgccctga cggtgaaaaa acgctatata gccaggctgg tgtccaatat
    22081 ttaatgctcg cgtggtcagg tcacctccct ggatatcttc cagcagtaac gcatcgattt
    22141 gtgcctgaga aaggaaaatc atcacagtgc ctcctgcggt acgctatccc accagatgag
    22201 cgcccagtcg cggctggcag gggcgagggc acgagcatct tgttgttgat accagtgata
    22261 taaccgctca cgctcatcgt cgttaagcgc gccaagactc cagctgacgt tctgctcaaa
    22321 acgttcccag gtgctgttat cctgttggca attttgtcca cggataaaat caacgtgggc
    22381 ataaattccc atctgataca ggacgttgag cgcaaagata tagttgggta attcgatcac
    22441 ttcccgacca gccgcccgct gaatggctgg agagacgaac gaggtgctga ccagatgagt
    22501 tgtatagacg cgaagccgtg cctgattatt cagtttgctc attgcatggc gcatatcagc
    22561 caccagagtg gagcgggaag ccacggcaat atcgcagcgt ggcaggtcac tccagtcctc
    22621 ttcccaggcg cgctggatcc agtggacatt gtcagccttc agtgcggcgg cgcggcgggc
    22681 ggcaacgttc agcatcccct gactgtaatc aacgccataa accgtagtga gtttgtcagc
    22741 cagcgccagc gatacggtgc ctggtccgca gcccatatcg aataaggtct gagcgccttg
    22801 caggtcaatt ttagcgatca gttgttgcag gtagctgtcc gttggcgagg cgcaattttc
    22861 cgccattttc tccgcccgtt gatcccagtg atcgggcgtt ttttctgtgc ggtgagccag
    22921 tttgagttgc tgcagataaa ggtctgcaaa atcaatatca tcaatgagca tgattgttcc
    22981 ttagcggcta attgcagaga ggtgatgatg aatttggtcg gcagaaaccc gatacagggc
    23041 cgccagtttg ttcgttgtta actgttcggt cggtaagcct tgtgttactc gtccatctgg
    23101 ctctacttgg ataatgctgt cggcaatggc gttggcatgg agcgtatggt gagtcgacat
    23161 taacattgtc atgccgttat ttttgagctg cgccagcgtg tccagaagtt ggatctgatg
    23221 accgaaatcg aggctggagg cgggctcatc cagtaacaac aagcgcggtt gttggacgag
    23281 tgcacgggcg attaaagcca gctgacgttc accgccgcta agcgtattcc agcgtcgtgt
    23341 cgccagatgc aaaatgccca gcttttccag ttgctccgtg gctttcaggc gttcttgctt
    23401 accgggaacg gaaaaagcgc ctatggtggg ggccaggccc atcaacacca tatccagcac
    23461 actgaaagca aaaataccgt cgtgcgcctg cggcacccag gcaatagcct gtgcgcgctg
    23521 gcgatggctg agtgtctgca cagagaggct atccagtagg atctcgcctt ttaataacgg
    23581 tataacgcca agaatggagc gcatgagtgt ggttttgccg caaccattcg cgccaagcag
    23641 acaacaaatt gttccttgcg acagggtgaa ggacacgtcg cgtagcactg gatggcggga
    23701 gtaacccaga gacgcatgac gaacggcaag caaagtcact ggcgacctcc gcgtaacaac
    23761 aaaagcagga aaaacggtgc gccaacaaaa gcggtcagaa tgccaagtgg gatttctgtt
    23821 gttccaatgc tgcgcgccag cgtatcggta agcaggagca gaatagcgcc agtacacatt
    23881 gccataggca acagctgctg atggttatga ccggtcaaca agcgggcaat gtgcggaacg
    23941 accagaccga tccaaccaat tatgcccgct attgctaccg tgctggcggt gattagtgtg
    24001 gcgcagacaa tcaatccaaa gcgcagacgc gtcacgttca gtcccagcga acgggcttcg
    24061 tcgtcggaaa gcgtgagtaa attcatgcgc cagcggagaa aaaacagggg caaggagcct
    24121 atgagaatga tcggtgcagc atagcagaga tcgcgtaagg tcacggtgga cagtcccccc
    24181 agcaaccaga aggtaattga cggtaactgg gtgtaaggat cggccagcgt tttaattagc
    24241 gatatccccg ctccgcacaa cgttcctaac gcaatgccta ccagcaccag ggttaagatc
    24301 ggatcgtggc gggtcacccg acgcgtaatc aaccagacgc ccgcgaccac catcagcccg
    24361 ccgcaaaatg cgtacagctg gatgtaaaca atgggtaatc cccacaggat tgccgcgcag
    24421 gcaccaagtc cggcaccggc ggagacgcct aaaatatccg gtgaaactaa gggattacgc
    24481 aacattccct gataggtggt tccggctcct gccagcgctg cgcccagtaa cagcgcagca
    24541 agaatacgcg gcacgcggat ttgccagaag acaatttgtt cctgcacaga ctcattttct
    24601 tgacgaagaa ttagcgccag cagttgctgc atatcgagat gataagcccc acttacggcg
    24661 gcaaaagtga tactgatagc tgccagcagc aaaatacatc cgtttacgat tctcattagc
    24721 tcgccaccaa tttttgatag tcggcatcac tgagtgaaca atgccagaac agttgggcgt
    24781 aatgctgcat gtcactttta aactggcgat tgatggcggg atccagccag gcgtgaagtc
    24841 tgcgtaatcc cagaagacgg ttgattcccg gcggggcatc gagccagcca aagggcaagc
    24901 cacttaaaaa taagatgcgt tgttccgcaa cggctttcac gccctgccag agaggatcac
    24961 gacgaataaa atctgcagta acggcctcct gaaccagaat aatatccggc tgccagcgca
    25021 ggagattttc catggaaacc tgtgtcaggc cgtggcgatc ggctatttgc gcgacgttgt
    25081 gcaaacctaa taattccgcc gcctcggtat gcaacgaacc ctgtaagccc gtttccagcc
    25141 ctcggggacc gcgcgcagca taaaagcgga ggttagcggc gggtgaggtg gcgaatgcct
    25201 gagcttcacc aacgaagtgg ctggcgagat tggcttgttc tgcggcgcgg tgctcttcgc
    25261 ctaacgtttt gccaagcgtt gttaactgtt ctgctgattg cgccagtttt ccgttaagca
    25321 ataaccaggg gatttgtgtc tgttcgctaa cctgccgtgc ctgggagatc aaggtttcat
    25381 ccgtattgcc gcaatcaacg accaaatcgg gatgtaacgc cataagccct tccagcgata
    25441 gtgtgctggc gcgcccggcg agtcgtccca gcctgggtaa ctggcgaatg tgctctggca
    25501 atggaattaa tgcctgacgg gcaaaatcaa aagaggaaaa accgaccatt ttttctggcg
    25561 cgacagccag caagagcaaa tcggcgggcg cgcctgcgct gacgatgcgt gtaatgtttt
    25621 gggcttcggg aagagattgc gaagcaaaac gattaactgt ttgtgaaaag gacggtagag
    25681 actgcaggag cagggtagat gcaagtgcct gcgcaaacgt ccgtctggtt atgggcatgg
    25741 tgaacatcc
//
LOCUS       DAJTYL010000054        25682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYL010000054 DAJTYL010000000
VERSION     DAJTYL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.054"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            320..882
                     /gene="srlA"
                     /locus_tag="OO572_002131"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             320..882
                     /gene="srlA"
                     /locus_tag="OO572_002131"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            879..1838
                     /gene="srlE"
                     /locus_tag="OO572_002132"
                     /gene_synonym="gutE"
     CDS             879..1838
                     /gene="srlE"
                     /locus_tag="OO572_002132"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6661130.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1849..2220
                     /gene="srlB"
                     /locus_tag="OO572_002133"
                     /gene_synonym="gutB"
     CDS             1849..2220
                     /gene="srlB"
                     /locus_tag="OO572_002133"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6661131.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            2224..3003
                     /gene="srlD"
                     /locus_tag="OO572_002134"
     CDS             2224..3003
                     /gene="srlD"
                     /locus_tag="OO572_002134"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6661132.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            3108..3467
                     /gene="gutM"
                     /locus_tag="OO572_002135"
     CDS             3108..3467
                     /gene="gutM"
                     /locus_tag="OO572_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6661133.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3534..4307
                     /gene="srlR"
                     /locus_tag="OO572_002136"
                     /gene_synonym="gutR"
     CDS             3534..4307
                     /gene="srlR"
                     /locus_tag="OO572_002136"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6661134.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4300..5265
                     /gene="gutQ"
                     /locus_tag="OO572_002137"
     CDS             4300..5265
                     /gene="gutQ"
                     /locus_tag="OO572_002137"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6661135.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5262..6776)
                     /gene="norR"
                     /locus_tag="OO572_002138"
     CDS             complement(5262..6776)
                     /gene="norR"
                     /locus_tag="OO572_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6661136.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6963..8402
                     /gene="norV"
                     /locus_tag="OO572_002139"
     CDS             6963..8402
                     /gene="norV"
                     /locus_tag="OO572_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6661137.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8399..9532
                     /gene="norW"
                     /locus_tag="OO572_002140"
     CDS             8399..9532
                     /gene="norW"
                     /locus_tag="OO572_002140"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6661138.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(9660..11912)
                     /gene="hypF"
                     /locus_tag="OO572_002141"
     CDS             complement(9660..11912)
                     /gene="hypF"
                     /locus_tag="OO572_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6661139.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(12065..12592)
                     /gene="hydN"
                     /locus_tag="OO572_002142"
     CDS             complement(12065..12592)
                     /gene="hydN"
                     /locus_tag="OO572_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6661140.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12741..13751)
                     /gene="ascG"
                     /locus_tag="OO572_002143"
     CDS             complement(12741..13751)
                     /gene="ascG"
                     /locus_tag="OO572_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6661141.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(13765..13884)
                     /locus_tag="OO572_002144"
     CDS             complement(13765..13884)
                     /locus_tag="OO572_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6661142.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(13894..15603)
                     /gene="hycE"
                     /locus_tag="OO572_002145"
     CDS             complement(13894..15603)
                     /gene="hycE"
                     /locus_tag="OO572_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6661143.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15621..16544)
                     /gene="hycD"
                     /locus_tag="OO572_002146"
     CDS             complement(15621..16544)
                     /gene="hycD"
                     /locus_tag="OO572_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6661144.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16547..18376)
                     /gene="hycC"
                     /locus_tag="OO572_002147"
     CDS             complement(16547..18376)
                     /gene="hycC"
                     /locus_tag="OO572_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6661145.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHS
                     LFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGE
                     WVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAE
                     NATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLL
                     IACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLK
                     LRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18413..19024)
                     /gene="hycB"
                     /locus_tag="OO572_002148"
     CDS             complement(18413..19024)
                     /gene="hycB"
                     /locus_tag="OO572_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6661146.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(19149..19610)
                     /gene="hycA"
                     /locus_tag="OO572_002149"
     CDS             complement(19149..19610)
                     /gene="hycA"
                     /locus_tag="OO572_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6661147.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19822..20172
                     /gene="hypA"
                     /locus_tag="OO572_002150"
     CDS             19822..20172
                     /gene="hypA"
                     /locus_tag="OO572_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6661148.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            20176..21048
                     /gene="hypB"
                     /locus_tag="OO572_002151"
     CDS             20176..21048
                     /gene="hypB"
                     /locus_tag="OO572_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6661149.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            21039..21311
                     /gene="hypC"
                     /locus_tag="OO572_002152"
     CDS             21039..21311
                     /gene="hypC"
                     /locus_tag="OO572_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6661150.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            21311..22432
                     /gene="hypD"
                     /locus_tag="OO572_002153"
     CDS             21311..22432
                     /gene="hypD"
                     /locus_tag="OO572_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6661151.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            22429..23439
                     /gene="hypE"
                     /locus_tag="OO572_002154"
     CDS             22429..23439
                     /gene="hypE"
                     /locus_tag="OO572_002154"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6661152.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23513..25591
                     /gene="flhA"
                     /locus_tag="OO572_002155"
     CDS             23513..25591
                     /gene="flhA"
                     /locus_tag="OO572_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6661153.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
BASE COUNT         6091 a         7140 c         6834 g         5617 t
ORIGIN      
        1 tgctgctaca ggcggcaagc aacacaaaaa ggggaagcaa tgttacataa cgacgcttga
       61 acatgagggg tccatttaac agattcaacc aggggcaagt atggtaaagc atcacacccc
      121 gcacaaggaa gcggtagtca ctgcccgata cggactttac ataactcaac tcattcccct
      181 cgctatactt ttattcaaac tttcaaatta aaatatttat ctttcatttt acgatcaaaa
      241 taacactttt aaacctttca acctgattag attaggttgc cgtttggtaa taaaacaata
      301 aatcctgaag gagagaacaa tgatagaaac cattactcat ggtgcagagt ggtttatcgg
      361 gctgttccaa aagggcggag aggtgtttac cgggatggtg accggcattc ttccgctgtt
      421 gattagcctg caggttatca tgaacgcact gattaatttt atcggtcagc atcgtattga
      481 acgttttgct caacgttgcg ccggtaaccc tgtttcccgt tacctactgt taccgtgcat
      541 tggcacgttt gtcttttgca atccgatgac cttaagcctg ggtcgcttta tgccggaaaa
      601 gtacaaaccc agctactacg cggcggcctc ttatagctgc cactcaatga atggcctctt
      661 cccccatatc aaccctgcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
      721 ctgaacctgc cacttggccc actggcggtg agttatctgc tggttggtct ggtcaccaat
      781 ttcttccgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
      841 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
      901 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
      961 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     1021 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     1081 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     1141 ttaatatcca ctcgacgggc aagtccggtc cgctggcgca gtacattgtg gaagatattt
     1201 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     1261 cttccgtggg ccgtgactat gacaccagta agaaaatcac cgaacaaagc gatggtttac
     1321 tggcgaaggt gggaatgggc atggggtccg ccgttgccgt gttgtttcag tctggtcgtg
     1381 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcattcgtc tcggcgctca
     1441 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctag
     1501 cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttccca ctgctttcac
     1561 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     1621 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     1681 aggcggcttg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     1741 ttcgcgtcgg tgtcccttct gtactggtga gccgcttttt aaccggcgcg ccgactgtac
     1801 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     1861 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     1921 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     1981 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat tttcactcgg gcagcatcgc
     2041 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     2101 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     2161 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     2221 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     2281 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     2341 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gtatggcgta cggttttggt
     2401 gctgacgcca ctagcgagca aagcgttctg gcgctctctc gtggggtaga tgaaatcttt
     2461 ggtcgcgtgg atttgctggt ctatagcgcc ggaatagcaa aagcagcctt tatcagcgac
     2521 ttccagctcg gcgattttga ccgttcgcta caagtgaatc tggtgggtta tttcctgtgt
     2581 gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat tattcagatc
     2641 aactcgaaat ccggcaaagt gggcagcaaa cacaactctg gctacagcgc agcgaaattt
     2701 ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg cattacggtg
     2761 cattcactga tgctcggtaa cctgctgaaa tcgcggatgt tccagtcact gttgccacaa
     2821 tacgcgacca agctgggtat caaaccggat caagtcgagc agtattacat cgacaaagta
     2881 ccgctcaaac gcggctgcga ttatcaagat gtgctgaata tgctgctgtt ctacgccagt
     2941 cctaaggcgt cgtactgcac cggacagtcg atcaatgtca ccggcggtca ggtgatgttc
     3001 tgatcaacag cggagatccg ttaaggatct ccgtgagact atagaatgcc tgatgcgcta
     3061 cgctcatcag gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac
     3121 tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt
     3181 ctcgttttaa ccgtgccttc gacacactat gccagcaagg gcgggttggc gtggaccgtt
     3241 ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgc gctggacgat cagcagtgca
     3301 tcgtcgacac cttgtttatg aaaggactga ctgtcttcgc ccgaccgcaa aaaattcccg
     3361 caattaccgg catgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac
     3421 tatcacagaa tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat
     3481 gttacttgct tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac
     3541 ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg
     3601 aagaattggc gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc
     3661 tggaacatgc cggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat
     3721 ccgatccgcc tatcgatcat aaaacgctca tcaacaccca caagaaagag ctgattgcag
     3781 aagctgccgt tagttttatc catgatggcg attcgatcat tcttgatgct ggcagtaccg
     3841 ttttgcagat tgttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagcc
     3901 tgcatatcgt caatgcgcta tccgaactgg ataacgaaca aactatcctg atgccaggcg
     3961 gaacatttcg caaaaaatcg gcctcatttc acgggcaact ggcagagaat gccttcgagc
     4021 atttcacctt cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa
     4081 ccacgttcaa cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga
     4141 ttttgatggc ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg
     4201 aaagcgtcga taagctaatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg
     4261 aagagaaagg gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac
     4321 acgggacgtc agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg
     4381 ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt
     4441 tcgggaattg gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc
     4501 ggcacaccgg ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt
     4561 gaaagccgcg atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg
     4621 attattccgc gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg
     4681 tcaccgctgg gcctggcggc gaaagcggtg ctggatatct ccgtagaacg cgaagcctgc
     4741 ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg
     4801 gcgatggcgg tcatgcaggc gcgcggtttt aatgaagaag attttgcccg ctcccaccca
     4861 gctggggcac tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg tcgtgatgac
     4921 gctatcccac aggtagcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc
     4981 accggtctgg ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc
     5041 gacggcgatt tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa
     5101 gcgatgacga caggcggcac cacattacag gcacaaagtc gcgccatcga cgccaaagaa
     5161 gttctgatga aacgcaaaat cactgccgca ccagtggtag atgaaaacgg caaactcacc
     5221 ggcgcaatta acctgcagga tttctatcag gccgggatta tttaatcctt caatcccaga
     5281 cgtttcgcca gccgatgcag gttggcgacg tcagtttcca gcattcgcgc acaggctgcc
     5341 cagttgtgat gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc
     5401 gcttcacgca ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc
     5461 acctcaggaa aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct
     5521 ctcgccagca ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt tcccggaaaa
     5581 ctgtagtgtt gcagtaaatt tcgcgccccg gcacttaata ccacgcggga gagcccctgc
     5641 cgcaaacgac actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca
     5701 cgcagcggcg gcaccgaaag tggaaacacg ctcaggcgat gaaacaaatc ggcgcggaat
     5761 cgccctgcca gcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg
     5821 acccgcaaac tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc
     5881 agcttggcct gcaatgccaa cgacaactcg ccgatctcat cgagaaacag agtaccgtta
     5941 tccgccattt caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca
     6001 tgcccgaaca actcactttc cgccacactt tccggcagtg cggcacagtt gagatagacc
     6061 agcggattca ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg
     6121 gttcccgtct caccgctgat cagaacgttg agatcggacg ccgccacaat ctcaatctct
     6181 tttttcagtt gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca
     6241 aacggcgtgg catcgcctgg cagcatattc tggctttcca gttgttcaat cagcaacgca
     6301 ttgcttaacg ctcccgccgc cagcgcggca atcagccgta actcttcgtc gctgaaaaca
     6361 tcgaactgat cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgtccgaca
     6421 aacaatggca gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg
     6481 tcatagggat cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca
     6541 atcgcttcca gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc
     6601 agaccgtcga tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca
     6661 tcgcactcca gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca
     6721 atcccacgct gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt
     6781 gcctcactgt caatttgact atagatattg tcatatcgac catttgattg atagtcattt
     6841 tgactactca ttaatgagca taattttatt tatagagtaa aaacaatcag ataagaaact
     6901 ggcacgcaat ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg
     6961 ctatgtctat tgtggtgaaa aataacattc attgggtggg tcaacgtgac tgggaagtgc
     7021 gtgattttca cggcacggaa tataaaacgc tgcgcggcag cagctacaac agctacctca
     7081 tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg
     7141 tgcagaacct gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg
     7201 cagaagagga ccacgccggg gcgctgaccg aactgatggc gcaaattccc gatacgccga
     7261 tctactgtac tgccaacgct atcgactcga taaatggtca tcaccatcat ccggagtgga
     7321 attttaatgt ggtgaaaact ggcgacacgc tggatatcgg caacggcaaa cagctcattt
     7381 ttgtcgaaac accaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg
     7441 cggtgctgtt cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg
     7501 atgaagtgga tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga
     7561 cgccgttcag ccgcctggta acgccgaaaa ttaccgagat cctgggcttt aacttaccag
     7621 tcgatatgat agcgacctcc cacggcgtgg tctggcgcga taacccgacg caaattgtcg
     7681 agctgtacct gaagtgggcc gctgattatc aggaagacag aatcaccatt ttctacgaca
     7741 ccatgtcgaa taacacccgc atgatggctg acgctatcgc ccagggaatt gcggaaaccg
     7801 acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga
     7861 ccaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga
     7921 tgccgaaaat cgccgggctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg
     7981 ccagtgcttt cggatctcac ggctggagcg gcggtgcgat ggatcgcctt tccacgcgcc
     8041 tgcaggatgc gggtttcgaa atgtcgctca gcctgaaagc caaatggcga ccagaccagg
     8101 acgctctgga gttatgccgt gaacacggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc
     8161 cgctgccgca gagcacggtg aatacggtag ttaaagaaga aacctctgcc acgacgacgg
     8221 ctgacctcgg cccacgaatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag
     8281 gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag tgaagtcccg gataacttcc
     8341 tctgcccgga atgctccctc ggcaaagacg tctttgacga actagcatcg gaggcaaaat
     8401 gagcaacggc attgtgatca tcggttcggg cttcgccgcc cgccaactgg tgaaaaatat
     8461 tcgcaaacag gacgccacta ttccattaac cctgattgcc gccgacagca tagatgagta
     8521 caacaaaccc gacctcagcc atgttatcag tcaggggcaa cgtgccgacg accttacccg
     8581 ccagacggcg ggtgaatttg ccgggcagtt taatctgcgc ctgtttccgc acacctgggt
     8641 gacggatatc gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga
     8701 caagttagtg ctggcaaccg gtgccagcgc ctttgtcccg cctgtgcctg ggcgtgagtt
     8761 aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc
     8821 ccgacgcgtg ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg
     8881 tcgtgcaggc aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat
     8941 gccaccagaa gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct
     9001 gttgaaatct cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga
     9061 ccaccagcgt agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac
     9121 agccctggca cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct
     9181 gcaaaccagt aatgccgata tttacgcgct gggcgattgc gcggaaatta acggtcaggt
     9241 attgccgttc ctgcagccga ttcaacttag cgggatggtg ctggcaaaaa atcttctcgg
     9301 caataacacg ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc
     9361 gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc tggcagatta ataccgaacg
     9421 ccagggaatg gtggcgcgcg gcgttgacga tgctgaccag cttcgcgcct ttgtggtcag
     9481 tgaggatcgg atgaaagagg catttgtatt gttgaaaacg ttgccggtgt aggtgggcta
     9541 ctgtgcctaa aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg
     9601 tgtaggccgg ataaggcatt tacgccgcat ccggcaaggg tgtccaaatg caacacgctt
     9661 tatccgttct ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga
     9721 gacaaaccgc catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga
     9781 taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc
     9841 gtagtgatac cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc
     9901 gcatcatgaa acgcccacgc gcgttgatta accagtgcct gccagttcag ccactgctgc
     9961 cagaaagtgg cgagatccag ttgattgtcc acccgcggca tcgtcaccgg atgcgtcact
    10021 ccgtcgcatg aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac
    10081 gtggctggcg cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgat
    10141 gccagcggcg cgttaattcc acgctcaatg gcccgcgcca gtacgctcca gttttgctgt
    10201 tgcacacttg ccgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc
    10261 agcaggtttc gccacggctg cttcgccgcc aaatcgccac ccggaagcgc cactgcaggc
    10321 aagccgccca ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa
    10381 gcgccattct cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc
    10441 agcggccact gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt
    10501 tgcgtcggca gattcatttc gctcgcccac tggctggaga catagcccgg atgcacatca
    10561 tgcacaatgt attgcggagt gaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc
    10621 cactgcatct ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt
    10681 tcaccgcgca ccaggcagaa ggtatttttc agatccgcgc caagacacag cacaggcgga
    10741 acatttttaa agcccggagg caaaaccagc gcatccggca catacccccg cgaacggcgc
    10801 agcatttcgc cgctttcgcg caccactgaa tcatccatcc gctgcacgat gtcgcggtta
    10861 tgtagcaaga atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata
    10921 gctggtggtt taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt
    10981 aacagcaaat gctggagcgg gttcgcaggc aacatcaccc cgacttcgtt aaggccaggg
    11041 gcgatatcat cacaaagctc aggtacgtat tttttatcca ccagcacaat cggcgcagcg
    11101 ggcgtggtaa gcaactggcg cgcagcgtct ggtaaacttt ctgccactgg caacatgact
    11161 gccagcggtt tcgccgggcg atgtttgcgc tcccgcagtg tcgccaccgc gttactgtta
    11221 cgtgcatcgc aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt
    11281 tttaactgtg cgatagctgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt
    11341 acccattcaa gatgcgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga
    11401 tcgagcgggt cgcggtactc tttgtcacag gccggacata gcggaaacgc cgccatcacg
    11461 gtaaacgggc ggtcgtaagg catcgcgcga ataatggtga aacgcgggcc gcagtgggta
    11521 cagttgataa acggataacg ataacgccgc tcgcctgggg tattcatttc ggcaaggcaa
    11581 gcagggcaag tggcggcatc cgggacaatt tgcgtattca tggtgccgcc ggtgctctgg
    11641 cggatggtga actcggtggg cagttgtgac cagataaacg gctcacgctc gacgctatca
    11701 atacgcgcca gtggcggaca gtgctgatgc aattgaacaa gaaacgtttc cgggtcttcc
    11761 agcagccgga cttctacgcc atcgccgtca ttacatacat cgccgtgaag atttaattgc
    11821 tgtgccagct gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc
    11881 agttggacac cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg
    11941 taacccgccc tgccggacac gacagcgtcg tatccggcag tctcaggtcg gcgataccgc
    12001 ccgctccgta ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag
    12061 atttttagaa catcagcgcc gtacggcggc gtttttctgc gctcagttgt tcaagtttat
    12121 tacgatcgac acaaatcagc gcatgggtcg ggcaagccgc catacacgcc gggccgtctt
    12181 cacgatggtt gcacaggtcg catttattgg cttcggcttt gtcagcccgt acattcagac
    12241 ccgcgccgct gttgcgaatc acaggacgta ccaccacttc catcgcgcca tacgggcaag
    12301 ccaccacgca ggttttgcaa ccaatgcaac gttcctgcat cacaggaaca aaccctttat
    12361 cacggctgat agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc
    12421 aaactgtcgc cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt
    12481 ccggggtcag cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac
    12541 aggtacggca accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct
    12601 ccagcaatga cagttaatgc gccgatacac tcacaaatca tgccagtttt taatttgctg
    12661 ttatttaagg aaattaattt ctgtaatgca ggaaaaaaga tgtcatcgac actagtgacg
    12721 atgacatgtg atgacaatgt ttatcgcgaa agggcaatga gtgagtcgcg gcggatcagt
    12781 tttccgctaa aggttttcgg cggtgagaaa tccccgccat cgagcataaa aatcagccgt
    12841 ccaataattt cctgaatcat ctcagtcacc ggaattttta cgctggagag cgccggaacg
    12901 atataggggg caattgcgat atcatcgaat ccgataactg acacctgctc tggcaccgct
    12961 acgccgcgct cgtgcaacgc tttgatcgca cctatcgcca tatcgtcgtt actggcaact
    13021 aacgcgctaa atttagcccc tcgttcgagc aacgtttcta ccccttcggc cccgctggcg
    13081 ggcgtccatt taccgttagc gataagtttt tcattgagcg caataccatg ctgcgacagc
    13141 gcgtctttat acccggcaag acgttcaatg ctggtggggg aatccatcga gccggtaagg
    13201 aaagcaatct cctgatgccc ggcgtttatc aactctgcca cggcgttaaa gctggtctgt
    13261 ttatgatcgc accagacgct atggctgcta ttattgcgca ggcggcgatt aagcaccatt
    13321 atcggctgac tgtgcgcgtc aatgatgtca tcaatctcat ccacgcttaa aaaacgcggg
    13381 taaatcataa tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg gcgctcttct
    13441 tcggcgctgt gtttaccatc tgccaatagc aactgtcgcc ctttctcttc cgccattcgc
    13501 gcggcatgaa acagtaattc actaaaataa atgccgtgat aaagcgtgtt ggtcactacc
    13561 agccccagcg tctgagtact cttcgccgac agattgcggg ccagcaaatt tgggcggtaa
    13621 ctgctctctt ctaccgcctg aaacacgcga tctttagtct cctggctgac gtagccatta
    13681 cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc cacttccagc
    13741 atcgtcgtca tcattttcat ccctttacac gcaatcaacg cagtgtactg caccgtttgc
    13801 cgattgtcct tgcgcaatcg gctccagcgg ataagacgag gtcgccgtgc cggttttgat
    13861 gacttttttg ataaaggtaa acatggcgat tccttatttc agcggcgagt ttttacgctc
    13921 aatgctgtaa cgctcgagtt ctttgtacgg caccactttg ctcttcttct tacgcacatc
    13981 gactacggtc atgcggtcgg tacaggagta gcaagggtcc aggctaccga taatcagcgg
    14041 cgcataggaa acggtattgc cacgcagcat gtagcgcagg gtcggccagt tcgcgtaggt
    14101 cgcggcacgg cagcgccagc ggtacagctt ctggttgtcg ccggtcatgc tccagtggat
    14161 atcgtcgccg cgcggcgctt cggcaaagcc cagcgcgaaa cggtgcggaa tgtaggtaaa
    14221 gccttccacc atcagcggac cgcccggcag gttatccaga ccgtagtcga tcatattcag
    14281 cgcggtatag acctcgttga ttcgcacttt cagacgggaa ataacgtcgc agccctgctc
    14341 gctgtggact tccattggca gcaggccata accgacaaac gggtgatcgg cgcgagtatc
    14401 gcgggcgaga ccgctggcgc gaaccatcgg gccaacgtta ctgaaatcgc gggcgatttc
    14461 cgggtccaga cgaccaatgc cgacggtgcg ttgttccatg ttcggcgtac tcagcagcac
    14521 atccaccagc tcctgcactt cacgacgcat ttgttgcgcc agctggcggg tctggatcat
    14581 gtcatctttc agcagatcgc gacgaatccc gccgatcagg ttcaggccgt aggttttacg
    14641 cgcaccggta aggatctctg ccattttcat ggaggtttca cgcacgcgga agaactgcat
    14701 aaagccggag tcgaagccgg taaagtgaca ggcgaggccg aggttgagca gatgcgggtg
    14761 caggcgttcc acctccagca gaatggcgcg gatcatctgc gcgcgttctg gcacctgaat
    14821 acccatcgcg ttttccaccg acgtggtgta ggcggtgctg tgggcaaagc cgcagatccc
    14881 gcacacacgg tcagagagga aggtcacttc gttataaccc atgcgggttt ccgccagttt
    14941 ttccataccg cgatggacgt agaacagacg gtagtcggcg tcgataatgt tttcgccatc
    15001 gacgaacaga cggaagtgac ccggttcgtc agaagtgacg tgcagcggac caatcggcac
    15061 gacgttgttt ttcttgtcgc ccagttcgtt gatgaactcg taggtttcag catcggtagt
    15121 cggtgccgga cgctgacgat aatccatgct gtctttacgc agcggataaa gttcatccgg
    15181 ccagtcatcc ggcagcacca gacgacgttc atccggcaga ccaaccggaa tcaaaccgta
    15241 catatcgcgc acttcgcgct cgccccacac cgccgccgga acgcgcggcg tcacggacgg
    15301 atactccggt ttgttggcgt caacttcgac gcgaaccgtt acccaacact tggtgccctt
    15361 ctccatcgac agcacgtaat aaacggcgta atgaccattc agtttgcgtt cgtcgttacc
    15421 aaacagcacc gacagccagc caccctgctt gtagtagaga aactccacca cttccggcag
    15481 atagttcacc tttacggtga cagtcagctg atctttggtc tgccaggcgt ggtccagcac
    15541 gacgcctaga aatgcctcat tcagcgcggc gagataatgt tgacctaatt tttcttcaga
    15601 catgctcaaa ctctctttaa tcacgccgcc agcaaggaga cgaacgctaa aaatgcaaag
    15661 ccaaacccag cccaggtaat gcgcggagta atatcaagac gcagacgggc catgctgttt
    15721 tcgaacagcg cgataaccag gacgccgacc accagtttga cgatggcaat caccagcgcc
    15781 agcagcagtc caccgacggt gaaggtttcc atttgtcccc acggaataaa caccccgacg
    15841 aacatctgca acaccaccag ctgtttcagg ctgatacccc atttcatgac gccaaagccg
    15901 ctaccgctgt attcggagag cggaccttcc tgcaactcct gctcggcttc cgccaggtcg
    15961 aacggcagtt tgcccatttc gataaaagtg gcgaacgcac aggcgcagag cgccagcacc
    16021 agcgggatgc tttggctcag cggccagtga taaacggtgt cggtaatgtt gctgatgttg
    16081 gtggaaccgg caacctgtgc ggcaacccac agaccaagca gcaacatcgg ttcgaccagc
    16141 acgccaagca tcgcttcacg gctcgcaccg atagcggtaa acgggctacc ggtatccaga
    16201 ccggaaatgg caaagaagaa acgcgcgatg gcgaagagat acagtaaggt gatcaaatcg
    16261 cccagttgcg gcagcggaga accgacggtc accaccggca gcgcagtagc gatagtcagc
    16321 atgacgccca ccatcacata cggcgtcagg cggaacatcc agccagaggc atccgggcca
    16381 acgctctggc ggcccagcag tttgataatg tcgcgatact cctgcaacac gcccggcccg
    16441 cgacggttat gcagacgggc gcgcgccacg cgggtaatac cggagagcag cggcgcaacg
    16501 gcaaataaca ccagcgcctg aattaacgga tataaaacac tcattctcag gctcctcgtg
    16561 aaacaataat caccaccagt accgccagtt caaccagcgc catccggcgg aacagcaacg
    16621 cactaccttc gcactgccag cccggcacta gcgacaccgg attcagccat ttgcgcagtt
    16681 tcagcactgg cgcaaacgcc tgtttaaccg gcatggcaaa accgtgagcg gtaatcacca
    16741 ttgatttttc atggtcgtag ccgcacaccc aggccgcacc gcgggaacgt gacggcaggc
    16801 ggtcgccttt gcaaatcgcc ataatgatga atggcagcag cgggcaggca atcagcagca
    16861 atgtgatcat tggttgagaa acggtggtgt tagcaggctc cagaggcaga ggtacagcag
    16921 cagagagcat cggcagcagc cacggcgcag caacaccgcc aattacgcag caaatcgcca
    16981 gtgccactac gcttacgctc atcaggagcg gcgcacaggt ggcgttttcg gcttctttgg
    17041 tacgcggcgc gccgaggaac gtgacgccat agactttcgc catacacatc accgccagcg
    17101 caccggtaat tgccagaccc acagcgagca gcggccccag cagacgggca acaaacgcgc
    17161 cgctattgct cagtttgaag aaggattgat agataaccca ttccccggca aacccgttca
    17221 gtggcggcag cgcagccatt gccatcaacc cgactaacat ggcgatggag ataaccggca
    17281 ttttcttgcc aataccaccg agtttttcga tatcgcgatg accggtacgg aaccagacgc
    17341 tccccgcccc gaggaacagt acgcttttga acaggctatg gttaagcaga tggtacagac
    17401 caccgaccag gccaagagca atcagcgccg gttgttcgag cgcgataccc gttacgccag
    17461 cgcccagccc cagccccagc cccagcccca gccccagccc cagccccagc cccagcccca
    17521 gcaggataat gccgatattt tccagggtgt ggtaagccag caggcgctgg atgttgtgtt
    17581 ccatcaacgc atacagacca ccgacgaacg cggtgatcat gccgagcacc agcagcgcga
    17641 tcccccacca cagcggtgca ttaccgccca gcagtgacag ggttaacatg cccagcaggc
    17701 caattttcat gactaccgta gaaaacaacg cggcagctgg cgcagaggcg ttagcatgtg
    17761 cctgcggcac ccagccgtgc agcggaataa tcccagccag caggccaaag ccaatcacac
    17821 cgagcagcca gatatcggaa ccgagcggca gctgttgcat acgcatatcc agcaggcgca
    17881 gatccagcgt gccataacgc tgccacagca gccagcaagc aatcaccagc agcagagtgc
    17941 caagacgcct cagcgcaaac cacagtttgc cctctttgct gttgctggtg aggaacaccg
    18001 cacacaaggc catgacttcg gccattacca cgaacatgcc gaggttgctg gcgatgacgg
    18061 cgcagacggc ggcagccatc aacatattga tctgcaagcc gttgcacttc acctgcgcgt
    18121 ggcgatgcca gtcaatgtta tagaggctga caaacagacc gcaaaggccg agggtaatca
    18181 gccaaatcgc gttaagcggg gagatttgca catcgtagct taccagcgac agcgcaccgc
    18241 tcacgccaac cgcgccagtc agtacagtga agcccgcggc tgccgtatac agactaccaa
    18301 ccgcgccgcc aattccggct atccagccac ttagcgcttt ttgaaaagag aaatgccaga
    18361 acagcggcgg cgacaaacca tgccacgccg ctattgatca gggaaattgc gctcatttag
    18421 cctctccact ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca
    18481 gctcacgctt acgtttgctg acgcgggcga tatcggtgtt atccaccaga tgcagggctt
    18541 tagtcgggca catccgcaca caggccggac cttgttcatc aaagctacaa aggtcacatt
    18601 tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag
    18661 caggcggtgc cggtggcgct ttcggggtat tggcgtttgc cggaatatcc agcggacggc
    18721 tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg cagcttacgc
    18781 acagactttc attcaactgc acggccccat caacgcgggt gatggcgtta accgggcaga
    18841 ccaccgcgca gggtgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat
    18901 tgagcatcac tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac
    18961 aggcggcctc acaggtgtgg cagccgatac agagcgtgga gtcggcaatt acaaaacgat
    19021 tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat
    19081 tttcgacact catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa
    19141 gcctcagctc atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca
    19201 ttgttttcaa tggcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt
    19261 tccagaacct gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc
    19321 agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg
    19381 atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg ttcaaaaagg
    19441 acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat
    19501 aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc actggtcctg taggcgacga
    19561 tgccgctgtg cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta
    19621 ctcgtttaac agagatgcca gctttaagca ttttttgtgc caacttttaa tttattgtta
    19681 ttaaagagat ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca
    19741 tcatcgacat tattcaccgc agggataatc aacactggcg caattattgc ttgtagctgg
    19801 caatagttaa tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg
    19861 atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt
    19921 ggcgcatttt cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc
    19981 ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt
    20041 gaaacatgcc aacagtatgt gacgctactg acccagcgcg tccgccgctg tccacagtgt
    20101 catggtgaca tgctacagat tgtggcagac gacggtttac agattcggcg gatagaaata
    20161 gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag
    20221 ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc
    20281 ccgcagatga aaatcaccgg cattaaagcg cctgaattta cccccagcca gactgaagaa
    20341 ggcgacctgc attacggtca tggcgaagcg ggcactcaca caccgggcat gagccagcgt
    20401 cggatgctgg aagtcgaaat tgacgtgctg gacaaaaaca accgtctggc tgaacgcaac
    20461 cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc tggtttccag ccctggttcc
    20521 ggtaaaacca ccctgctgac ggaaacctta atgcgcctga aagacagcgt tccgtgcgcg
    20581 gttattgaag gcgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggtaca
    20641 ccagcgattc aggtgaacac cggtaaaggc tgccatcttg acgcacagat gattgccgac
    20701 gccgcaccgc gtctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac
    20761 ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca aagtagcggt gctttccgtt
    20821 accgaaggtg aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg
    20881 ctgctcaaca aagttgacct gttgccgtat ctcaactttg acgttgagaa gtgcatcgcc
    20941 tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggcgaa
    21001 gggatggacc agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc
    21061 agatccgcac cattgacggc aaccaggcga aagtcgacgt ctgcggcatt cagcgcgatg
    21121 tcgatttaac gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg
    21181 tactggttca cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc
    21241 tcgacgcctt acaaaacatg tttgacgttg agccggatgt cggcgcgctg ttgtatggcg
    21301 aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat
    21361 tgagcatctg cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat
    21421 tatggaagtg tgtggcggtc atacccacgc catttttaaa ttcggcctcg accagttact
    21481 gcctgaaaac gttgagttta tccacggtcc gggttgcccg gtgtgcgtac tgccgatggg
    21541 tagaatcgac acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg
    21601 cgacgccatg cgcgtaccag ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc
    21661 cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac
    21721 ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc atgccgacca ccgccatcac
    21781 tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcacattac
    21841 gcttatcccg acgctgcgca gtttgctgga acagccggat aacggtatcg acgcgttcct
    21901 cgcgccgggc cacgttagta tggttatcgg cactgatgcc tataatttta tcgccagcga
    21961 ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt gatctactgc aaggcgtggt
    22021 catgctggtg gagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgggt
    22081 ggtaccggat gccggtaacc tactggcgca acaggcgatt gccgatgtgt tctgtgtcaa
    22141 cggcgacagc gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc acctgacgcc
    22201 ggattatcaa cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga
    22261 tgacccgcgc gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc atcaatgccc
    22321 gctgtttggt aacacctgta atcctcaaac cgcatttggt gcactgatgg tctcctccga
    22381 aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt gaataatatc
    22441 caactcgccc acggcagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg
    22501 gaagcctttg ccaacccgtg gctggcagag caggaagatc aggcacgtct tgatctggcg
    22561 cagctggtag cggaaggcga ccgtctggcg ttctccaccg acagttacgt cattgacccg
    22621 ctgttctttc ctggcggtaa tatcggcaag ctggcgattt gcggcaccgc gaatgacgtt
    22681 gcgggcagtg gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg
    22741 ccgatggaga cactgaaagc cgtagtgacc agcatggcag aaaccgcccg cacggcaggc
    22801 attgccatcg ttactggcga tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt
    22861 atcaacaccg ctggcatggg cgcaattccg gcgaatattc actggggcgc acagacgcta
    22921 accgcaggcg atgtattgct ggttagcggt acactcggcg accacggggc gactatcctt
    22981 aacctgcgtg agcagctggg gctggatggc gaactggtca gcgactgcgc ggtgctgacg
    23041 ccgcttattc agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt
    23101 ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct gcggttgtgg tattgaaatt
    23161 tctgaatcag cgctgccggt taaacctgcc gtgcgcggcg tttgcgaatt gctgggactg
    23221 gacgccctga actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg caacgcggca
    23281 gagcaagtgc tggcagcgtt acattcccat ccactgggga aagacgcggc gctgattggt
    23341 gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc
    23401 gatttaccac acgccgaacc gcttccgcgt atatgctaat aaaattctaa atctcctata
    23461 gttagtcaat gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata
    23521 tacaccgatg agtgatctcg gacagcaagg gttgttcgac atcactcgga cactattgca
    23581 gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct
    23641 cgccgacaac gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc
    23701 gtcgcgtgaa aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc
    23761 ggtacgcagc attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga
    23821 aacctggccg cagctggccg caggtgggct ataccccaaa tttggtcact attgcctgat
    23881 gccactggcg gcgggagggc atatttttgg tggctgtgaa tttattcgtt atgacgagcg
    23941 tccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt
    24001 caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac tatgagttgt tatgccggga
    24061 gcgcgataac ttccgcatcc tggttgccat taccaacgcg gtgctttccc gcctggatat
    24121 ggacgaactg gtcagcgaag tcgccaaaga aatccattac tatttcgaca ttgacgatat
    24181 cagcattgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca ctcactatct
    24241 tgataaacaa catcccgccc acgaacagag cgaagtcgat gaagccggaa ccctcaccga
    24301 acgcgtgttc aaaagtaaag agatgctgct gatcaatctc tacgagcggg acgatttagc
    24361 cccctatgaa cgcatgttgt tcgacacctg gggcaaccag attcaaacct tgtgcctgtt
    24421 accgctgatg tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa
    24481 agtgtttacc actaccaatc tgaatttgct gcgccagatt gccgaacgtg tggcaatcgc
    24541 tgtcgataac gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga
    24601 aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg
    24661 ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag
    24721 taccgtgctg atcctcggtg aaactggctc gggtaaagag ctgattgccc gtgcgatcca
    24781 taatctcagt gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc
    24841 cggattgctg gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccaacgc
    24901 ccagcgcatt ggtcgttttg aactggcgga taaaagctct ctgttcctcg acgaagtggg
    24961 cgatatgcca ctggagttac agccgaagtt gctgcgtgta ttgcaggaac aggagtttga
    25021 acgtctcggc agcaacaaaa tcattcagac ggacgtacgt ttaattgccg cgactaaccg
    25081 cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt accgcctgaa
    25141 cgtattcccg attcacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc
    25201 gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc
    25261 cgagacgctg cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa
    25321 cgtcattgag cgcgcagtat tgctaacacg cggcaacgtg ctgcagctgt cattgccaga
    25381 tattgcttta ccggagcctg aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga
    25441 agatgaatat cagttgattg tgcgcgtgct gaaagaaacc aacagcgtgg ttgccgggcc
    25501 taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg
    25561 gctgggaatt gataaatcgg cattgattta actgcaaatt gccggacaga tctgcctgtc
    25621 cggcatacta ttcacgaggt tttttcggac gatatttttc cggcagttct ggcaccggac
    25681 gc
//
LOCUS       DAJTYL010000055        25580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYL010000055 DAJTYL010000000
VERSION     DAJTYL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.055"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1276)
                     /gene="yeaG"
                     /locus_tag="OO572_002156"
     CDS             complement(<1..1276)
                     /gene="yeaG"
                     /locus_tag="OO572_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6661154.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAF"
     gene            1712..2458
                     /gene="mipA"
                     /locus_tag="OO572_002157"
     CDS             1712..2458
                     /gene="mipA"
                     /locus_tag="OO572_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6661155.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2548..3309
                     /locus_tag="OO572_002158"
     CDS             2548..3309
                     /locus_tag="OO572_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6661156.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3360..4244)
                     /locus_tag="OO572_002159"
     CDS             complement(3360..4244)
                     /locus_tag="OO572_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6661157.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4328..5323)
                     /gene="gapA"
                     /locus_tag="OO572_002160"
     CDS             complement(4328..5323)
                     /gene="gapA"
                     /locus_tag="OO572_002160"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6661158.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5665..6078
                     /gene="msrB"
                     /locus_tag="OO572_002161"
     CDS             5665..6078
                     /gene="msrB"
                     /locus_tag="OO572_002161"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6661159.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6120..6392
                     /locus_tag="OO572_002162"
     CDS             6120..6392
                     /locus_tag="OO572_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6661160.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6762..7838
                     /locus_tag="OO572_002163"
     CDS             6762..7838
                     /locus_tag="OO572_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6661161.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7865..9244
                     /locus_tag="OO572_002164"
     CDS             7865..9244
                     /locus_tag="OO572_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661162.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9261..10306
                     /locus_tag="OO572_002165"
                     /pseudo
     CDS             9261..10306
                     /locus_tag="OO572_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10327..11163
                     /locus_tag="OO572_002166"
     CDS             10327..11163
                     /locus_tag="OO572_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6661163.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11168..12115
                     /locus_tag="OO572_002167"
     CDS             11168..12115
                     /locus_tag="OO572_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6661164.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12125..13105
                     /gene="ydjG"
                     /locus_tag="OO572_002168"
     CDS             12125..13105
                     /gene="ydjG"
                     /locus_tag="OO572_002168"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6661165.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13242..14000
                     /locus_tag="OO572_002169"
     CDS             13242..14000
                     /locus_tag="OO572_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6661166.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14118..15476
                     /locus_tag="OO572_002170"
     CDS             14118..15476
                     /locus_tag="OO572_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661167.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15569..16210)
                     /gene="pncA"
                     /locus_tag="OO572_002171"
     CDS             complement(15569..16210)
                     /gene="pncA"
                     /locus_tag="OO572_002171"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6661168.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPKHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16221..17237)
                     /gene="ansA"
                     /locus_tag="OO572_002172"
     CDS             complement(16221..17237)
                     /gene="ansA"
                     /locus_tag="OO572_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6661169.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17404..19260)
                     /gene="sppA"
                     /locus_tag="OO572_002173"
     CDS             complement(17404..19260)
                     /gene="sppA"
                     /locus_tag="OO572_002173"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6661170.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19421..19972
                     /locus_tag="OO572_002174"
     CDS             19421..19972
                     /locus_tag="OO572_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6661171.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20089..21132
                     /gene="selD"
                     /locus_tag="OO572_002175"
     CDS             20089..21132
                     /gene="selD"
                     /locus_tag="OO572_002175"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6661172.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21137..23098
                     /gene="topB"
                     /locus_tag="OO572_002176"
     CDS             21137..23098
                     /gene="topB"
                     /locus_tag="OO572_002176"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6661173.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23225..24265
                     /locus_tag="OO572_002177"
     CDS             23225..24265
                     /locus_tag="OO572_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6661174.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24382..>25580)
                     /gene="gdhA"
                     /locus_tag="OO572_002178"
     CDS             complement(24382..>25580)
                     /gene="gdhA"
                     /locus_tag="OO572_002178"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6661175.1"
                     /translation="LLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKG
                     GMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLM
                     TELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPE
                     ATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGT
                     VVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQN
                     ELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLE
                     MAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAM
                     LAQGVI"
BASE COUNT         6498 a         5951 c         6600 g         6531 t
ORIGIN      
        1 taaacgcaaa acgcgtcgac agaccgttca tcccttcatc gacaccggcg tagtcacgat
       61 attcctgata cgacttggct ttcggatcgg tgtctttcag actttcgcca tcataaaccc
      121 gcatctttga ataaatgctg gagttttctg gctctttcag gcgcgaaaga atggaaaaac
      181 gtgacagtgt ttcgagcgtg ccaggggcgc atggggcgtg agtcaattca ctgtgattaa
      241 gcaatttctc gtagattttg atctcttcgg aaatgcgcaa gcaatacggc accttcacga
      301 tgtaaacacg gtcgaggaag gcttcgttgt ttttgttatt acggaaagtg acccattcgg
      361 acttgttcga gtgggcgaga ataatcccgt tgaacggcag ggcggagata ccttccgtcc
      421 cgttgtagtt accttcctga gtggcggtta acaagggatg cagcactttt atcggtgctt
      481 taaacatctc aacgaattcc atgatcccct gattggcgcg gcacagcgca ccggaatagc
      541 cgtaggcgtc cgggtcattc tgcgcgtagt gttcgagttt acgaatatcg actttcccaa
      601 ccagcgcgga gatgtcctgg ttgttctcat caccgggttc cgttttggcg atagcaattt
      661 gttgcagaat tgacggccag accttcacta cccggaactt agtgatatcg ccaccaaatt
      721 catgcaggcg ttttgccgcc catggcgaca tgatggtgcc gagataacgg cgaggaatgc
      781 catactcttt ttccagaatc tgagcatctt cctgcggatt gaaaagacag aacggatgat
      841 cgttgaccgg gctacgctca ccgttggcgc tcaatacata aatcggtacg agctgcatta
      901 atgatttcag tcgctcagca agcgatgatt tacccccacc cacaggcccc agcagataca
      961 ggatttgttt cttctcttcc agcccctgag ccgcgtgttt cagataagag acaatctgtt
     1021 caatcgcgtc ttccatgccg taaaactctt caaatgccgg ataacgtgca atgacccggt
     1081 tagaaaagag tcgagaaagt ctggggtcct gggctgtatc gaccatgaca ggctcaccga
     1141 tagccatcaa tagccgctca gccgcgttgg cataagcact gcgatcttgc cgacaagtgg
     1201 taagaaactc ctgcagtgtg aactcttcgt ccttggcagc ttcatatcgc tggcgatagt
     1261 gatcgaatat attcatggta tgccgtcctt tcgtttttta gcacaggtta agagccgttc
     1321 atatgaatag tagaggctcc cggaagaggt aaactgaacg acgtctcttg ctaaaacaca
     1381 gcaactctca tgccatttta tcgacgatga gaacaacgtg cccggtgata caccttcaaa
     1441 attaagcgta gatggcaaat ggaaaacttg cctgcacggc tttcttaatt tcaatggcat
     1501 atcaacaact catccataaa ttttgcataa ttaatgtaaa gaccaggctc gccagtaacg
     1561 ctaaatccat ttggctgtaa gcgcggtgtc atccgcggca ggaaaattaa acagttactt
     1621 taaaaaatga aaacgtaaaa aggttgggtt tcgatgtatt gacgggtaaa ctttgtcgcc
     1681 cgctaaacat ttgtttaagg aatgattaat tgtgaccaaa ctcaaacttc tggcacttgg
     1741 agtgcttatc gcaacgtctg caggcgtagc gcacgctgaa ggtaaatttt ccctgggcgc
     1801 aggcgtaggt gtcgttgagc acccatataa agattacgat accgatgttt acccagtacc
     1861 ggtaatcaac tatgaaggcg ataacttctg gttccgtggc ttaggtggtg gttactacct
     1921 gtggaatgac gcaacggata aactttcaat taccgcttac tggtcgccgc tttacttcaa
     1981 agcgaaagac agtggcgatc accaaatgcg tcacctggat gaccgtaaga gcaccatgat
     2041 ggctggtctg tcttatgctc actttaccca gtacggttac ctgcgtacca ccctggctgg
     2101 cgataccctg gataacagca acggcattgt ctgggatatg gcctggttgt atcgttacac
     2161 caacggtggc ctgaccgtga ctccgggtat tggtgtgcag tggaacagcg aaaaccagaa
     2221 cgaatactat tatggcgtat cgcgcaaaga gtccgctcgc agcggtctgc gtggctataa
     2281 cccgaatgac agctggagcc cttaccttga gctgagcgcc agctacaact tcctcggcga
     2341 ctggagtgtt tacggtaccg cgcgctacac ccgtctgtct gatgaagtta ctgacagccc
     2401 gatggtggat aaatcctgga ctggcctgat ttctaccggg atcacctaca aattctgata
     2461 atgcatcttt ccagggcatt ttgtgcataa aaacagggcg tcacgccctg ttttgcatta
     2521 cggtggtgca atacctggga ggaaacaatg caacaaaaaa tgattcaatt tagtggcgat
     2581 gtctcactgc cagccatagg gcagggaaca tggtatatgg gcgaagatgc cagtcagcgc
     2641 aaaacagaag ttgctgcact acgcgcaggc attgaactcg gtttaaccct cattgatacc
     2701 gccgaaatgt atgccgatgg cggtgccgaa aaggtggttg gggaagcatt aaccggtctg
     2761 cgagagaagg tctttctcgt ctctaaagtc tatccgtgga atgctggcgg gcaaaaagcg
     2821 ataaatgcat gcgaagccag tttacgccgt ctcaatactg actatctcga tctctactta
     2881 ttacactggt ctggcagttt cgcttttgaa gagactgttg ctgcgatgga aaaattgatc
     2941 gcccagggaa aaatccgtcg ctggggcgtt tctaaccttg gttatgccga tatgcaggaa
     3001 ctctggcagc tgccgggggg aaatcagtgt gccactaatc aggtgcttta ccatctcggt
     3061 tcacgaggga ttgagtacga tctactcccc tggtgccagc aacagcagat gccggtgatg
     3121 gcttacagtc cattagccca ggccgggcgg ttgcgcaatg gactgttaaa aaacgcggta
     3181 gtcaacgaaa ttgcacatgt ccaacaacat gcagctgcgc ttgaggtcga actttcgtca
     3241 gcggaattag ctatgctgga taaggcatat ccggcaccaa aaggaaaaac tgcgctggat
     3301 atggtgtgat gttgatacgt gccggatgca tcgcacccgg cacacaacat taacgcaaat
     3361 taacgtttgg caacgcgaat ggattgcgcc agatgtgcag gtttctcttt ggtgactttt
     3421 tgcgtttctg aagcgtaagc cgtttctaca caaacaaagg tcttgtagcc atcgtccggc
     3481 atgtcgccca tgctaactga aagcgccggt cccgggttcc agccgacaac gttcagatga
     3541 tgctggtggc ctacggcgat aatacgattc agcgcttcat cattaatcac gctgcaatct
     3601 tgcggattca gatacacgcg atcggtacgg tcagggaagg tctgaatacc gtcggtcagt
     3661 acatcttctt tcgcgtcatt cactttatca atgaagcgat cgcccagccc actgacgctt
     3721 accttagcga tatcacccac gttaaagtag gtatgcaggg cagaggtggt ttcaaattcg
     3781 ccatgtgatt caagatcgat ttcacaggtt ttacccacgc ggaaatgcgc taacagcgta
     3841 aagtcgtgcg gccagaattt tttcgtctct tcgctttgtg tcaattcaaa agtcagcgct
     3901 acgccatcag catcttcatg atgtgatttc agcgtccacg gcaggttgcg ggcaaaaccg
     3961 tgcgcaggca gaccttgttg tgccgccgga ccaaaccacg gccagcaaac cggtacgcca
     4021 ccgcgaatag cgacgccatt tttgaacggt gtgttgttgc tcaaccacag aacttcttct
     4081 tcacccgcag gtttccacga gagaaggtgt gcgccctgta atgcaaaaga ggcttttacc
     4141 tggggatgat cgaccacaat gaggtccagt tcatccagtt tacgacggga gaggacaggg
     4201 gagatttgtt cgatgaccgg aagggcaaaa attttcttaa tcatgacgca gtcctttaac
     4261 ttcattttat caggtaaaaa aaagagcgac cgaagtcgct ctttttagat cacagtgtca
     4321 tctcaactta tttggagatg tgagcgatca ggtccagaac tttgttggag taaccggttt
     4381 cgttgtcgta ccaggatacc agtttcacga agttgtcgtt cagagcgata ccagctttag
     4441 catcgaacac ggaagtgcaa acttcgccgt tgaaatcggt agatactacg tcatcttcgg
     4501 tgtagcccag aacgcctttc atttcgcctt cagcagcagc tttaacggca gctttgatct
     4561 gctcgtaagt tgcagctttt tccagacgaa cggtcaggtc aactacggat acgttcgggg
     4621 tcggaacgcg gaacgccata ccagtcagtt tgccattcag ttctggcagt actttaccta
     4681 cagctttagc agcaccggta gaggacggga tgatgttctg ggaagcgccg cggccgccgc
     4741 gccagtcttt gtgagacggg ccatcaacgg ttttctgagt agcggtagta gcgtgaacgg
     4801 tggtcatcag accttcgatg atgccgaagt tatcgttgat aactttagcc agcggagcca
     4861 ggcagttggt ggtgcaggaa gcgttggaaa cgatgtcctg gccagcatat ttgtcgaagt
     4921 tagcgccttt aacgaacatc ggagtgttgt ctttagacgg accagtcata accactttct
     4981 tcgcaccagc ggtgatgtgt ttacgagcag tttcgtcagt caggaacaga ccagttgctt
     5041 cagcgacaac gtcaacaccc acttcgtccc atttcaggtt agccggatca cgttcagcgg
     5101 taacacggat ttttttaccg ttaacgatca gatgaccgtc tttcacttca acggtaccgt
     5161 cgaaacggcc gtgagtggag tcatatttca gcatgtatgc catgtaatca gcgtctaaca
     5221 ggtcgttgat tgcaacgatc tcgatgtcag aacgtttctg agcagcacgg aaaacaatgc
     5281 gaccgatacg gccaaaaccg ttgataccta ctttgatagt catatattcc accagctatt
     5341 tgttagtgaa taaaaggttg cctgtaaaat tacaaaaacc ttacgcagcg tcaagcggaa
     5401 tcgtgtcaat cattgcgaca aatcaatcct gtgcctaagc attacgcgac tgactcgcct
     5461 cacttttcct ttgggcttga gaccacatgg ggtcggcgcc ccgaatttta aagggcaatt
     5521 acgataaaaa tgtgattttc atcacgattt aggtgcagcc atttcgtcga gatcaagttt
     5581 aggacaaaat tttgccagtt ggtgttaatg ttttgttaga atcagtcacg taatgtgagc
     5641 acgattaaag tgagatgtga gtaaatggcg aataaacctt cggcagaaga actgaaaaaa
     5701 aatttgtccg agatgcagtt ttacgtgacg cagaatcatg ggacagaacc gccatttacg
     5761 ggtcgtttac tgcataacaa gcgtgacggc gtatatcact gtttgatctg cgatgccccg
     5821 ctgtttcatt ccgaaaccaa gtatgattcc ggctgtggct ggcccagttt ctacgaaccg
     5881 gtgagtgaag aatccattcg ttatatcaaa gacttgtcac atggaatgca gcgcatagaa
     5941 attcgttgcg gtaactgtga tgcccatctg gggcatgtct ttcccgacgg gccgcaacca
     6001 acaggcgaac gttattgtgt taactctgct tctttacgct tcactgatgg cgaaaacggc
     6061 gaagaaatca acggttgaaa aaacgattca gcaacttatt ccacaggagc ggaaaaatta
     6121 tgaatcttga tgacattatc aacagcatga cgcctgaggt ataccagcgt ttgtcgaccg
     6181 ccgttgaact ggggaaatgg cctgatggcg ttgcgttgac cgaggaacaa aaagagaatt
     6241 gcctgcaact ggtgatgctg tggcaagccc gccataatac cgaagcacag catatgacaa
     6301 ttgacacaaa tggtcagatg gtcatgaaga gcaaacagca gttaaaagaa gattttggta
     6361 tctcagcaaa gcctattgcc atgtttaagt aagtggttca tccccttttt gccgcggcta
     6421 tgaaggggat agccaacaat gaccccacga ctcgtctcaa ttatctcgac ccgacctggc
     6481 taagtcccag tccgatctct tgcagtcgcg ttcacgcctg aatgacttct cctgaactta
     6541 agtctcagac ctatttggcc ggtaatccct ctcgaatttg ctctttttgc gtcccgcctt
     6601 ccaccaacct gtaacagacc cagccatttt ctgcgtaatt gtgatttgag ttgtaaaatg
     6661 caggtcatta tctgtgttgt gcagatttaa tattgacctc aaaaatcatg tgggtaagta
     6721 tgatggccat ctaagcaaaa caaaacacag aggatgacgc aatgaaagca ctggctcggt
     6781 ttggcaaggc ctttggcggc tacaagatga ttcatgtccc acaacccatt tgtggcccgg
     6841 aagacgtagt gactgaaatt aaagccgcgg caatctgcgg cgcagacatg aagcactaca
     6901 atgtcgatag cggttctgat gagtttaact ctatccgcgg ccatgagttc gcaggttgta
     6961 ttgcgcaggt tggtgaaaaa gtcaaagact ggaaagtggg gcaacgcgtc gtgtcggata
     7021 acagcggtca cgtttgcggc gtttgtccgg cctgtgaaca gggtgatttt ctgtgttgta
     7081 cagaaaaagt aaaccttggt ctggataaca atacctgggg cggtggtttt tccaaatatt
     7141 gtctggttcc tggtgaaatt ctcaaaattc atcgtcatgc gttgtgggaa atccctgatg
     7201 gtgttgatta tgaggacgca gccgtacttg accctatctg taatgcctat aaatccatcg
     7261 cgcagcaatc gaaattcctt cctggtcagg atgttgtcgt cattggcact ggcccactcg
     7321 ggctgttttc cgtacaaatg gcgcggatta tgggggcggt aaatatcgtc gtcgttggtc
     7381 tgcaagaaga tgtggcggtc cgcttcccgg ttgcaaaaga actgggggcg acggcagtag
     7441 taaatggttc taccgaagat gtggtggcgc gctgccagca aatttgtggc aaagataatc
     7501 tgggactggt gattgaatgc tccggtgcca atatcgcact gaaacaagcc atcgaaatgc
     7561 tccgcccgaa tggggaagtg gtacgcgttg gaatgggctt caaacctctt gatttctcga
     7621 ttaatgacat taccgcctgg aataaaagca tcattgggca tatggcctat gactccacct
     7681 catggcgtaa cgctatccgg ctattagcca gcggcgctat caaagtcaaa ccgatgatca
     7741 cgcatcgtat cggcctgtcg caatggcgcg aagggtttga tgcgatggtc gataaaaccg
     7801 caatcaaagt gatcatgact tacgactttg atgaataact actctcctga aggaacgtta
     7861 tcgcatggaa cagataacaa aaccgcattg tggtgcacgg ctggatcgct taccggattg
     7921 ccgctggcat tcgtcaatgt ttgccatcgt cgcgtttggc ttgcttgtct gctggagtaa
     7981 tgccgttggt ggcttgatcc tcgcgcagct gaaagcgttg ggctggacag ataattccac
     8041 tacagccaca ttctcagcaa tcacgaccgc cggaatgttt ctcggtgcgc tggtgggcgg
     8101 catcattggc gacaaaaccg gtcgcagaaa tgcgttcatc ctctatgagg ccattcatat
     8161 tgcctcgatg gttgtcggtg ctttctcacc gaatatggat tttcttattg cctgccgttt
     8221 tgtgatggac gttgggctgg gagctttact ggttacactg tttgctggtt tcaccgaata
     8281 tatgcccggt agaaatcgcg gaacgtggtc cagtcgggtt tcctttattg gcaactggtc
     8341 atatccgctc tgctcactga ttgcgatggg actcacgccg ctgattagtg cagagtggaa
     8401 ctggcgggta caactgctta ttcctgcaat attgtcgctt atcgctacgg cgctggcctg
     8461 gcgctacttt cctgaatccc cgcgctggct ggaatcgcgc ggacggtatc aggaagccga
     8521 gaaagtcatg cggagtatag aagaaggcgt catacgccag acgggtaaac ctttgccgcc
     8581 tgtggttatt gctgatgacg gtaaagcgcc acaagcggtg ccgtattcag ccttactgac
     8641 aggagtatta ctgaaacgcg tgatattagg ctcttgtgtg ctgattacca tgaacgttgt
     8701 gcagtacaca ctaattaact ggttgccaac aatattcatg acccagggga ttaatttaaa
     8761 agactcgatt gttttaaata ccatgagtat gtttggcgcg ccatttggta tttttattgc
     8821 catgctggtg atggataaaa ttccgcgtaa aacaatgggt gtggggctat taatcctgat
     8881 tgcggtgctc ggatatatct attcactgca aaccagtatg ttgctcataa cgctgattgg
     8941 tttcttcctg attactttcg tctatatgta cgtttgctat gcctcggcag tgtatgtccc
     9001 tgaaatctgg ccgacagagg ccaaactccg tggctccggt ctggcgaatg cggtagggcg
     9061 aatcagtggt attgccgcac cttatgccgt tgcagtgctg ctcagtagtt atggtgtaac
     9121 gggagtcttt attcttctgg gggctgtttc aaatattgtc gcaattgcta tcgccaccat
     9181 tggaattgaa accaaaggtg tctccgttga aagtttaagt attgatgcag tagccaataa
     9241 ataatacggg tggattcaaa atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa
     9301 tgaaaattat ttcagcagaa ataccagtgc ctaaagaaga tgaagttttg attaaagtag
     9361 aatatgtcgg tatttgtggt tcagatgtac atggttttta atcaggcccg tttattccgc
     9421 ctaaagaccc aaatcaagaa attggcctgg gtcatgaatg cgccgggacg gttgtggctg
     9481 tgggaagccg cgtgcgcaaa tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc
     9541 cttgcggtca ctgtcgttac tgtctggaag gcaaatataa catctgcccg gacgttgatt
     9601 ttatggcgac acaacccaac taccgcggcg cattaacgca ctatctgtgt catccggaga
     9661 gctttactta caaactgccc gacaatatgg acacgatgga aggggcgctg gtggagcctg
     9721 ccgcagtcgg gatgcatgcc gcgatgctgg cagatgttaa accgggtaag aagataatta
     9781 ttctgggagc aggttgtatt ggtttgatga cgttgcaagc gtgcaaatgc ctgggcgcaa
     9841 cggaaattgc cgtcgttgat gtactggaaa aacgtctggc aataggggaa cagcctggtg
     9901 cgacagtggt tattaatggc gcaaaagagg acactattgc acgctgtcag caatttaccg
     9961 aagacatggg cgcagatatt gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg
    10021 cacctttaat ctggtaatgc gcggcggtaa aattatgatt gttggtactg tacccggcga
    10081 ttcggcaatc aatttcctca aaatcaatcg cgaagtcact atccagacgg tattccgcta
    10141 tgccaatcgt tatccggtca cgattgaagc tatttcttca gggcgattcg atgtgaaatc
    10201 gatggtgacg catatttacg attatcggga tgtacaacag gcatttgaag agtcagttaa
    10261 caacaaacgc gacattatta aaggcgttat taaaattagc gattaattaa tacgggagaa
    10321 cagaacatgc tcgcagatat cagatattgg gaaaacgatg ccacaaataa atattatgca
    10381 attgcccatt ttaacgtatg gaatgcagaa atgttgatgg gcgttaaaga cgcagccgaa
    10441 gaagcgaaat ccccggttat tatttctttt agtacaggtt ttgtcggtaa cacctcattt
    10501 gaagatttct ctcacatgat ggtatcaatg gcacaaaaag caacggtgcc ggtaataact
    10561 cattgggatc atggtcggag tattgagatt attcataacg cctggactca tggtatgaat
    10621 tcattaatgc gtgatgcttc tgcatttgat ttcgaagaaa atattcgttt aaccaaagag
    10681 gctgttgatt tctttcatcc gctgggtatt ccggtagagg cggaattagg gcatgtcggt
    10741 aatgaaaccg tttatgagga agcgctggcg ggctatcact ataccgatcc tgaccaggcg
    10801 gctgaatttg ttgaacgtac gggctgtgac tcattggctg tcgccatcgg caaccagcat
    10861 ggggtttata cgtcagagcc gcaattgaac tttgaggtcg tcaaacgcgt acgcgatgct
    10921 gtttctgttc cgctggtttt gcatggcgca tcggggatca gtgatgccga cattaaaact
    10981 gcaatttcgt tgggtattgc gaaaatcaac attcatacgg agctctgtca ggccgcaatg
    11041 gtagcagtga aagaaaacca ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa
    11101 gctgtaaaag aacgggcatt ggaaaaaatt gaactgttcg gttctgatgg caaagcggaa
    11161 taatgatatg gataatctcg acgttatttg tataggtgcc gctattgttg atattccatt
    11221 acaaccggtc agtaaaaata tctttgatgt agattcttat cctcttgaaa gaatcgcaat
    11281 gagcaccggg ggcgatgcaa taaatgaagc aacaattatt tctcgtctgg ggcatcgtac
    11341 cgcattaatg agccgcattg gtaaagatgc ctcagggcaa tttattctcg accattgtcg
    11401 caaagagaat attgatattc aaagcctgaa acaggacgtc agcatagata catctattaa
    11461 cgttggactg gtgacggagg atggcgagcg gacgtttgtc acgaaccgta acggcagttt
    11521 gtggaaatta aatattgacg atgttgattt tgcgcggttt cctcaggcga aattattatc
    11581 actggccagt attttcaaca gtcctctatt ggatggtaaa gcgctaacag aaatttttac
    11641 gcaagcgaaa gcccggcaga tgatcatttg tgccgatatg atcaaaccgc ggttgaatga
    11701 aacgctggat gatatttgcg aagcattgag ttatgtcgat tatctgtttc caaattttgc
    11761 cgaggaaaaa ttactcacag ggaaagagac actggatgaa attgctgact gctttcttgc
    11821 gtgcggcgta aaaacggtgg tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg
    11881 tgacatgacg atgaaggtgc cggcggtcgc aggaataacc gccatcgaca ccattggcgc
    11941 gggcgataac tttgcttcag gttttattgc ggcactgtta gaaggcaaaa atctgcgtga
    12001 atgcgcacgc tttgccaatg caacggcggc tatctcggtt ctaagcgtcg gtgccaccac
    12061 cggcgtaaaa aacagaaagc tggtcgaaca attgctggaa gaatacgaag gataatgaag
    12121 gcaaatgaaa aagatacctt taggcacgac ggatataacg ctttcgcgaa tggggttggg
    12181 gacatgggcc attggcggcg gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat
    12241 tgatacgatt cttgaagccc atcgttgtgg cattaatctg attgatactg cgctaggata
    12301 taactttggc aatagtgaag ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca
    12361 ggttgtagta gaaaccaaat gcggcattgt ctgggaacga aaaggaagtt tattcaacaa
    12421 agttggcgat cggcagttgt ataaaaacct ttccccggaa tctatccgcg aagaggtaga
    12481 agccagcttg caacgtctgg gtattgatta catcgatatc tatatgacgc actggcagtc
    12541 ggtgccgcca ttttttacgc cgatagctga aactgtcgca gtgcttaatg agttaaaggc
    12601 tgaagggaaa attcgcgcta taggcgctgc taacgtcgat gctgaccata tccgcgagta
    12661 tctgcaatat ggtgaactgg atattattca ggcgaaatac agtattctcg accgggcaat
    12721 ggaaaacgaa ctgctgccgc tatgtcgtga taatggcatt gtggttcagg tttattcccc
    12781 gctagagcag ggattgttga ccggcactat cactcgtgat tacgttccgg gcggcgctcg
    12841 ggcaaataaa gtctggttcc agcgtgaaaa catgctgaaa gtgattgata tgcttgaaca
    12901 gtggcagcca ctttgtgctc gttatcagtg cacaattccc actctggcac tggcgtggat
    12961 attaaaacag agtgatttaa tctccattct tagtggggct actgcaccgg aacaggtacg
    13021 cgaaaatgtc gcggcactga atatcaactt atcggatgca gacgcaacat tgatgaggga
    13081 aatggcagag gccctggagc gttaaatact caacacgatt cacctctgtt tctgtgaaaa
    13141 ctgtgatgaa aatctgtgat tcaaataggt tattttgaaa gtaaacatcg gttatgcgat
    13201 aatcgcgcta atgtgatgta aaaagataca ggggtgtaat cgtggcggca aaagacagga
    13261 ttcaggcaat taagcaaatg gttgccaacg ataaaaaggt gacagtctca aatttgagtg
    13321 ggatttttca ggtaaccgaa gaaaccattc gccgcgatct tgagaagctg gaagatgaag
    13381 gctttttgac cagaacctat ggtggtgctg ttttaaatac agcggtgttg acggagaata
    13441 tccattttta taagcgcgct tcatcgtttt atgaagagaa gctgcttatt gcacgcaagg
    13501 cactaccctt tatcgacaat aaaaccacca tggcagccga ttccagtagt accgtaatgg
    13561 aattgctcaa attgttacag gaccgtagtg acctgacgtt gctaaccaac tcggcggaag
    13621 cgattcatgt tctggctcag tcagaaatta aagtcgtttc aacgggtggg gaactaaaca
    13681 aaaatacact ttccctgcaa ggaagaatta ctaaagagat catcagccgc taccatgtcg
    13741 atatcatggt aatgagttgt aaaggtcttg atattaacag cggcgcgctg gactctaatg
    13801 aagcggaagc tgaaatcaaa aagacaatga tccgtcaggc gacagaagtt gcgttattgg
    13861 ttgatcactc taagtttgat cgtaaagctt ttgtccagtt agctgacttt agtcatatta
    13921 attatataat aactgataaa tcaccgggtg cagagtggat agcattttgc aaagacaata
    13981 atatccagct ggtgtggtaa tttaagcaca cgttgtcaat attatttgtt cctgcgactg
    14041 gaaataaaat gatgataaag atgcggcaag tgaatttgcc gcaattgcta ccttttatag
    14101 caaatccgag gcacccaatg gaacaatatg atcaaattgg cgcaagactg gaccgcttgc
    14161 ctttggcccg gtttcattat cgtatatttg gtataataag ctttagtctg ttattaacgg
    14221 ggtttttgag ttactcaggt aatgtcgtct tagcaaagct ggtaagcaat ggatggtcaa
    14281 ataatttcct ctatgccgcc tttacctcgg cattaatgtt tggttatttc atcggctcac
    14341 ttactggtgg gtttattggt gactactttg ggcggcgcag ggcgtttcgc ataaatcttc
    14401 tcatcgtcgg tattgctgca acaggggccg cttttgtccc tgatatgtac tggctcattt
    14461 tctttcgctt cctgatggga acaggaatgg gggcgctgat tatggttggc tatgcctcat
    14521 ttacggagtt tatccccgcg acggtgcgtg gaaaatggtc cgcgcggctc tcatttgttg
    14581 gtaactggtc gcccatgctg tctgcggcga taggcgtggt ggttatcgct ttttttagtt
    14641 ggcgaataat gtttcttctg ggtggtattg gcatactgtt agcctggttt ctctcaggta
    14701 aatactttat cgagtcgcca cgatggctgg aagggaaagg gcaaatcgcc ggtgcagaaa
    14761 gccaacttcg tgaagtagag cagcaaattg aaagggagaa gagtattcgt ttaccccagc
    14821 ttactttgaa ccagagcaac agcaaggtta aggtaatcaa gggtaccttc aggctcctgt
    14881 ttaaagggga aatgttacga cgtacattag ttgcgataac tgttttaatt gcaatgaaca
    14941 tttcgcttta taccattacc gtatggatac cgaccatatt tgttaactcc ggcattgatg
    15001 tcgataaatc aatattaatg accgctgtta ttatgattgg tgctccggta ggaatattta
    15061 ttgcggcatt aattattgat cattttcctc gtcgattatt tggctccgcc ttacttatta
    15121 ttattgccgt gttaggctat atctattcaa gtcagactac agagtgggcg attttaatct
    15181 atggtctggt gatgatcttc tttttataca tgtatgtttg cttcgcgtcg gcggtttata
    15241 tcccggagct ttggccaacg catttacgcc tgcgcggttc gggtttcgtt aatgccgtcg
    15301 gacggatcgt cgcagttttc acgccctatg gcgttgcggc attattaaca cattatgggt
    15361 cgatcacggt gtttatggtg cttggtgtca tgttagtgct ctgtgcgctg gttctctcca
    15421 tttttggcat cgaaacgcgg aaggtgtcgc tggaagagat ttctgaggtg aattaaatct
    15481 ttcgttgatg gcatcaattg ccagatgtac cactgacgtg ttttatttgg cctacaatgc
    15541 cagtacagaa ttgtaggcca gcgtaaaatt acccctgcgt ctcttcccag tctgccagcg
    15601 tatatagcgt tgccccagct gctgacatct ccataaacgc gtgtgcactg tcctggggct
    15661 ggatattcac gccacgacag ccatcggtaa tcacgtttac cttataacct aactgtaacg
    15721 cgtccagcac ggtaaacttc acgcaatagt cagtagccag gcccataacg atcaattcat
    15781 cgatttcatg atcgcgcaac cagtcatcaa gcgcggtttt ctgccgacgt ccgttatcaa
    15841 aaaaggcact gtaactgtca actaaaggat tttcgccttt atggaacacc gctgcgatct
    15901 ctttttgatt cagtaacgga tgtaattgtg cgccttcact gttctgcaca cagtgtttag
    15961 gccagaaagt ttgccgcagg ccgccgagtt ggcctggcgt ataaggctct acgccgtgct
    16021 ggctggcaaa gctgccgtga ttcgccggat gccagtcctg gctggcgata accgcttctc
    16081 cgcgcgactg gcaccagtca ataaggcggt tagcaacctc caccgtactg tcaccttccg
    16141 gtacggcgag cgcgccacca gcacagaaat cattttgtaa atcgaccagt aacagggcgc
    16201 gatggggcat tgcagtctcc ttaatcatcc ggcgtcagtt cgccgcgcag gttttggctc
    16261 atggccttgc gaatggtttc agtatccagt tcctggctca gcaggtaatg cagtttggtt
    16321 agcgtggctt ctacagtcat atctgcaccg tcaataacgc cggcatgggc gagggcgtta
    16381 ccggtggcat aaccactcat attcacttta ccggacatac attgtgtcag gttgaccacc
    16441 acaataccgc gatcgctggc ttcttgtaat tcctgcagga aggctttgtt ttgtggcgca
    16501 ttacccacgc cataggagcg cagaatcaat gctttcaccg gttggcgcag aaaattgcgc
    16561 acgacgtcag cagaaatccc tggataaatc gtcaccacgc caattggttg tggcgtgatt
    16621 ggatgaacga ttaattcccc ttcaccgtgc ggggcgggtg gcgtattcaa acgacgaata
    16681 tggataccag cttccagtaa cggaggaagg tttggagagg caaacgcatc aaaaccatcg
    16741 gcatgggctt tggtagtgcg gttgccgcga tacaatcggt tattgaaaaa gagcgttact
    16801 tcgttgatcg gataattcgg cgcaacgtac aacgcattca gcagattaat ttgtccgtca
    16861 gagcgtaact cagccagcgg gatttgtgac cctgtcacaa tgaccggttt accgagattc
    16921 tcgagcatga acgacagcgc agaggcggta tacgccatcg tgtcggtacc gtgtagaatg
    16981 acaaaaccat catagtcgtc atagtgcgct ttaatatctt cagcaatatg ctgccagtct
    17041 tccggcgtca tatcagaaga atccatcaac ggcgtatatt catgaatggt gaaatctggc
    17101 atctccgggc gatggaattc cggcatcagc gccagttggc gttgtagatg acctgacacc
    17161 ggaatataac cctgctcgga acgctgcatc ccgatggtcc cgcccgtgta ggcgacgtaa
    17221 attgatttct tttgcatgat attgatatga atgatatcga aagagcaaaa gtatatacgt
    17281 gaggcaggag gagcgcacct gatactatgc ggataaggaa atatggggcg agacagcggg
    17341 aagaggattc gtcgaggtaa aaaataatgt ggccgacgat ctgtcggcca ctcagtacaa
    17401 gacttaacgc acgttggcgc aggtcaggca aaacgcataa cggttttgtg ggtcgttaaa
    17461 cgcggccagc ttgtcgcttt cacttttaac ggtagaggct accgaggcaa gcggtgcagg
    17521 taacatggcc tggaacgcat ctggcaacat tgcccggaca gaaccagaca tgttgtccat
    17581 cactttgtcg aagaaggtcg gttcatcaac gtagtattcc agatgccact gtttcacttt
    17641 tgcgagctct gctgctttgg caaccgcatc atcgaaatcc ccgagactat cgaccagccc
    17701 gttagctttt gcatcctgac cggtccagac gtggccctgg gcgattttat caatttgctc
    17761 cggcgtcgaa tgacgcgcat cagcaaccag cgtgataaag cgtttatagc cattctcaat
    17821 gcttaattgc atcatctgct gcgcttccgg cggcagtgcc ctggtgatag aaacatccgc
    17881 cagcggtgaa gttgagacac catcagtatg aacaccaatc gaatccagac tattttctac
    17941 ggtggtgatc acgccgaaga taccgataga accggtcagg gtgctggggt tagccacaat
    18001 gtaattagct ggcgtggaaa tccagtaacc accagatgcc gccatgccgc ccatcgatac
    18061 aaccacagac ttacccgctg cccgggctgc tgccagttca gcgcgaatca cttcagacgc
    18121 ggtaacgctg ccgcctgggc tattaacacg caggacaatc gctttcactt tcgggtcaag
    18181 gcgagcgtcg cggatttgtg ccgcagtggt atcaccgcca acattcccct gagtttcctc
    18241 gccatccata attgcgccat tagcgaagac gacaccgatg ctgtcaccgg tatctgccgg
    18301 cgttttcaat gcgtaatcgt aataactgat ggcgcgataa tttttatcag tcttactcca
    18361 gccgaactct ttggtcagag ctttttcgat ttcggcactc gatgccagtg catcgaccag
    18421 cttgttttcc agtgcatatt tcgcggtatc gccaccggtt ttggttaaac cctcaagcaa
    18481 cccttgcgcg ccagggaata cctgctgagc agggatctgc cggttagcgg caacagtatt
    18541 cagatagttt tgccacagct caccaatcca gcggctgtca gcttcgcggg ctgccggtga
    18601 catatcatca cgaataaacg gttcaacggc agatttatac gtacccacgc ggaacacatg
    18661 ggtggaaact ttcagcttat ccagcaacga tttgtagtac agaccgttgg tagcaaaacc
    18721 gtgcagatca accacgcctt gcggtgacag ccaaatttta ttggcgaaac tggcgagata
    18781 atattgcccc tggctgtagt tctcgccaac ggcataaacc ggtttcccgc tgtcacgaaa
    18841 ctctttcagg gctttgccga tgtactgcat agacggctgg tcgccgcctg cgaagttttt
    18901 cagatccatc acgataccgg tgatattgcg gtcgtccttc gcctggcgaa tggtgttgac
    18961 gatatcaaac agtgagtttt cctgcagacg atcggaactg gcaccaagca gctggcggct
    19021 taatttacta aaccgctgag aactgtctgg tttatcgacg atcacaccag aaatgtccag
    19081 cagcagtgcg ccacgactgg ccgtttcttt cgaatcacca ccactgacct gcatccagat
    19141 ccccacacca accagtacga ggaaaataaa gaacaggtta agtaccattt cacggacgaa
    19201 attcagcaga cgccacgtcc atttaaaaaa tccggcaata aatcgccaaa gggttcgcat
    19261 gtattctccc aacttaaggt cacacctgtc acaggcgcaa tagatgtggt tatcgtaatg
    19321 acccaaccgg cacttgtcag caggaattgc ggactgtgct gtaacaaaat ccactctcgt
    19381 gttaattttg tgaataaata tcacgacagg agttaatcaa atggatgcac tcgaactatt
    19441 gatcaatcgc cgtagcgcct cccgcctggc tgaacccgcg ccaacgggtg aacaactgca
    19501 aaacatcctg cgtgcgggta tgcgtgcgcc ggaccataag tccatgcaac cgtggcattt
    19561 ttttgtgatt gaaggggaag ggtgcgagcg tttcagcgcc gtactggaac agggggcgat
    19621 tgctgccggt agtgatgata aagctatcga caaagcccgt aatgcgccgt tccgcgcacc
    19681 gctcatcatc acggtggtgg cgaaatgcaa agaaaatcat aaagtcccgc gatgggaaca
    19741 ggaaatgtct gccggatgcg cggtcatggc gatgcaaatg gcagccgttg cccagggatt
    19801 tggcggcatc tggcgcagtg gcgcattaac tgaaagtccg gtagtgcgtg aagcattcgg
    19861 ttgccgtgag caggataaaa ttgtcggttt tctctacctc ggcacgccgc agttgaaagc
    19921 atctacgtcg attaacgtcc cggacccgac gccgtttgta acttatttct gataattgtc
    19981 gcgaaactgt ctggattgtg agcaaaggcg caggaattca gacactctca cttatcactt
    20041 cacggaatga gggttaccat acccggattg cattgacagg agatgtccat gagcgagaac
    20101 tcgattcgtt tgacccaata cagccacgga gctggttgcg gctgtaaaat ttccccaaaa
    20161 gtgttggaaa ccatcctgca cagtgagcag gcgaagtttg ttgatccgaa tttgcttgtg
    20221 ggtaatgaaa cccgcgacga tgcggcggtg tacgatctgg gcaatggcac cagcgttatc
    20281 agtaccaccg acttctttat gccgatcgtt gataatcctt tcgattttgg ccgcattgcg
    20341 gcgactaacg ccatcagcga tatcttcgcg atggggggca aaccgattat ggcgattgcg
    20401 atcctcggct ggccgattaa caaactttcc ccggaaattg cccgcgaagt aaccgaaggt
    20461 ggacgctatg catgccgtca ggcgggtatt gcgctggctg gcggtcactc catcgatgcg
    20521 ccggagccga tttttggtct ggcggtaacg gggatcgtac cgaccgagcg ggtgaagaaa
    20581 aacagtaccg cacaagccgg atgcaaactg ttcctgacga aaccgctggg gatcggcgtt
    20641 cttaccacgg ctgagaaaaa atcactgttg aaaccagaac atcagggact ggcgacggaa
    20701 gtgatgtgcc ggatgaacat cgcaggcgcg tcctttgcca acatcgaagg cgtaaaagcg
    20761 atgaccgacg ttacgggctt tggtctgctg ggccacttga gcgaaatgtg tcagggggct
    20821 ggtgtgcagg cacgcgtcga ctatgaagcg atcccgaaac tccccggtgt tgaagagtac
    20881 attaagttgg gcgcagtacc tggcggcact gaacgtaact ttgccagcta cggtcatctg
    20941 atgggtgaaa tgccgcgtga agtgcgcgat ctgctgtgcg atccgcaaac ttctggcggt
    21001 ttgctgctgg cggtcatgcc ggaagcagaa aatgaggtta aaactacagc cgccgagttt
    21061 ggcattgaac tgacggcgat tggcgaactg gtgccagcgc gcggcggtcg tgccatggtt
    21121 gagatccgtt aatttaatgc ggttgtttat tgccgaaaaa ccgagtctgg cgcgcgccat
    21181 tgctgatgtc ctgcccaaac cgcaccggaa aggcgatggc tttatcgagt gcggtaatgg
    21241 tcaggtggtg acttggtgta tcggtcacct gcttgagcag gcgcagccag acgcctacga
    21301 cagccgctat gcgcgctgga atcttgcgga tttgccgatt gtcccggaaa agtggcaatt
    21361 acagccacga ccctccgtga ccaaacaact taacgtcatc aaacgcttcc tgcatgaagc
    21421 cagcgaaatc gttcacgccg gggacccgga tcgtgaaggg caattgctgg tggatgaagt
    21481 gctggactat ctgcaactgg caccggaaaa gcgccagcag gtgcagcgtt gcttgataaa
    21541 cgacctgaac ccgcaggcgg ttgagcgggc gatcgaccgt cttcgttcca acagtgagtt
    21601 tgtgccattg tgcgtttctg cgctggcgcg agcgcgtgcc gactggctgt acggcatcaa
    21661 tatgacccgt gcgtatacca ttctcggtcg caatgccggt tatcagggcg tactttccgt
    21721 gggacgcgtg cagacacccg tgctcgggct ggtggtgcgc cgcgatgaag agattgaaaa
    21781 cttcgtggcg aaagacttct ttgaagtcaa agcacatatc gtgacacctg ccgatgagcg
    21841 gtttaccgct atctggcaac cgagcgaagc gtgtgaaccg taccaggatg aagaagggcg
    21901 cttgttacat cgtccactgg cggagcatgt ggttaaccgc attagtggtc aaccggctat
    21961 tgtcaccagc tataacgata aacgggaatc agaatccgcg ccgctgccgt tttcgctttc
    22021 ggcgttgcag attgaagcgg caaaacgctt cggtctgagc gcgcagaacg tgcttgatat
    22081 ctgccagaag ctgtacgaaa cgcacaagct aatcacttat ccgcgttccg attgtcgcta
    22141 tttgccagaa gaacattttg ccggacgcca cgcggtgatg aatgccatca gcgttcatgc
    22201 accggatctg ttgccgcagc cagtggtaga tccagatata cgcaaccgct gttgggatga
    22261 caaaaaggtc gatgcgcacc acgccatcat cccgaccgca cggagttctg cgatcaacct
    22321 gacggagaac gaagcgaagg tctataacct gattgcccgt caatacctga tgcagttctg
    22381 cccggatgcg gtgttccgca agtgtgttat cgaactggac attgccaaag gcaaatttgt
    22441 cgctaaagcc cgttttcttg ctgaagcagg ctggcgcacg ctgttaggca gcaaagagcg
    22501 cgatgaagaa aacgacggca cgccattgcc tgtggtggcg aaaggcgatg agttgctgtg
    22561 tgaaaaaggt gaagtggtag agcggcaaac ccagccgccg cgccatttta ccgatgcaac
    22621 actgctttcg gcgatgaccg ggatcgcgcg ctttgtgcag gacaaagatc tgaaaaaggt
    22681 ccttcgtgcg accgatggtc tggggacaga agcaacccgt gccgggatta ttgaactgct
    22741 gttcaagcgt ggttttctga ccaaaaaagg gcgctatatc cactccaccg acgccggaaa
    22801 agcgttattc cattcgctgc cagaaatggc gacgcgaccg gacatgaccg cgcactggga
    22861 atcggtgctg acgcaaatca gcgaaaagca gtgtcgctat caggacttta tgcagccgct
    22921 ggtggggacg ctatatcagc ttattgatca agccaaacgt acgccggtgc ggcagtttcg
    22981 cggcattgtg gctccgggca gtggtggcag tgctgataag aaaaaggctg caccgcgtaa
    23041 acgtagtgcg aaaaaaagtc cgccagcaga tgaagccgga agcggggcga tagcgtaagc
    23101 gagcgaatct ttcgtgctat tcgagtcata ttctgaaata tccagcggat caagaaagtt
    23161 cgttggatat ttttttgcat ggataaaatt atcgcctcta aagtatgtaa taacagggaa
    23221 tgtgatgaaa aaagttttat tgcaaaacca tcctgggagc gagaagtatt cttttaatgg
    23281 ctggggaata tttaatagta attttgaacg ggtgattaaa gaaaataagg ccatgctgct
    23341 ttgtaagtgg gggttttatt taacatgtgt tgtcgctgta atgtttgtat tcgcagcgat
    23401 aacatccaac ggtttgaatg aaagaggcct gattaccgcg ggatgctctt ttctttatct
    23461 attaattatg atggggctta ttgttcgggc cggttttaaa gcaaaaaaag aacaactgca
    23521 ttattatcag gctaaaggta ttgagccgct cagtatcgag aagttacagg cgctacaatt
    23581 gatcgcacct tatcgattct atcataagca atggtctgaa acgctagagt tctggccgcg
    23641 aaagcctgaa cctggcaaag ataccttcca atatcatgtg cttccttttg actcgatcga
    23701 tatcatcagt aaaagacgcg agtctttaga gaatcaatgg ggtatcgaag atagcgaaag
    23761 ttattgtgcc ttaatggagc attttctttc tggcgaccat ggagccaata cctttaaagc
    23821 aaacatggag gaagccccag agcaggttat cgccttgttg aataaatttg ctgtttttcc
    23881 ctcagactat atctctgatt gcgctaataa tcgctccggt aaatcctcgg cgaagctaat
    23941 atgggcggcg gaattatcat ggatgatctc gatatcaagc acagcttttc aaaacgggac
    24001 aattgaagaa gaactggcct ggcattatat aatgcttgct tctcgaaagg cgcacgagtt
    24061 gttcgaaagc gaagaagatt atcaaaaaaa tagtctaatg ggatttcttt actggcatat
    24121 ctgctgctat cgcagaaagt taacggatgc tgaacttgaa gcgtgttatc gttacgacaa
    24181 gcagttttgg gagcactaca gtaagaaatg ccgttggccc ataagaaatg ttccctgggg
    24241 agcatcatcc gttaaatact cataaacgcc tgaaattttg ccgggggcgc tgcgcttgac
    24301 cggcctacat tagagcgtaa ctgcaatgaa ttgtgcccat ctgtaggcct gataagcgta
    24361 gcgccatcag gcatttacaa cttaaatcac accctgcgcc agcatcgcat cggcaacctt
    24421 cacaaaaccg gcaatgttcg cgccctgcac gtagttggtt tgctcacctt caccaccatg
    24481 ctcaacacag gcatggtgga tatccagcat gatgtgatgc aaacgtgcgt caactttctc
    24541 ggctttccag cccaggcgcg cagcgttttg tgccatttcc aggcccgacg ttgctacacc
    24601 gccagcatta gccgctttac ccggtgcaaa cagtacgcct gcctgctgga acagttcagt
    24661 cgcttcgatg gtggtcggca tatatgcccc ttcagcgacg gctttaacgc cattggcgat
    24721 aagctgatgc gcggcgtcaa catccagttc gttctgggtg gcgcaaggca gggcgatatc
    24781 aaccggtaca gaccacggct gttggccttc gagatagacc agaccaaatt ctttggcgta
    24841 atctgccgct cgaccatcgc ggctggcttt gatttcgata agacgtgcca gtttctcttt
    24901 cgtgaatccg ctttcatcaa ctacagtgcc gctggagtct gacgcagtga tcacacgagc
    24961 accaaattcc atcgcttttt cgatagcgta ctgggcgacg ttgccggagc cagaaacgga
    25021 aacgctcatc ccttcaaaac ccataccgtg gcgttttaac attgcttctg tgaaataaac
    25081 cagaccgtag ccggtagctt ccgggcgaat aagactaccg ccaaatgaaa ggcccttacc
    25141 ggtgaagacg caggcggtat tgttggagag ctttttcatc atccccgcca taaagccgac
    25201 ttcacgacca ccaacgccga tatcacctgc cggaacgtcg gtatccgcgc ccaggtggcg
    25261 atacagttca gtcatcagcg tctggcaaaa acgcatcact ttaccttcgc tttttccttt
    25321 cggatcgaaa tcgctgccgc ctttaccacc gcccatcggc agggtagtca gggcattttt
    25381 gaaggtttgt tcaaagccga ggaatttgag aatggaaagg ttaactgacg gatggaagcg
    25441 cataccgcct ttgtacgggc cgatggcaga gctgaactgc acacgccatg cacggttgac
    25501 ctgtatctgg ttgcgatcat caacccatac cacgcgaaac tggatcacgc gctccggttc
    25561 aaccagacgc tccagtaatg
//
LOCUS       DAJTYL010000056        24771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYL010000056 DAJTYL010000000
VERSION     DAJTYL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.056"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            570..1652
                     /locus_tag="OO572_002179"
     CDS             570..1652
                     /locus_tag="OO572_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6661176.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2141..2939)
                     /locus_tag="OO572_002180"
                     /pseudo
     CDS             complement(2141..2939)
                     /locus_tag="OO572_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2949..3500)
                     /gene="ybcL"
                     /locus_tag="OO572_002181"
     CDS             complement(2949..3500)
                     /gene="ybcL"
                     /locus_tag="OO572_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6661177.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3669..4001)
                     /gene="emrE"
                     /locus_tag="OO572_002182"
     CDS             complement(3669..4001)
                     /gene="emrE"
                     /locus_tag="OO572_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6661178.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4346..4660)
                     /gene="fliE"
                     /locus_tag="OO572_002183"
     CDS             complement(4346..4660)
                     /gene="fliE"
                     /locus_tag="OO572_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6661179.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4874..6533
                     /gene="fliF"
                     /locus_tag="OO572_002184"
                     /pseudo
     CDS             4874..6533
                     /gene="fliF"
                     /locus_tag="OO572_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6526..7521
                     /gene="fliG"
                     /locus_tag="OO572_002185"
     CDS             6526..7521
                     /gene="fliG"
                     /locus_tag="OO572_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6661180.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7514..8200
                     /gene="fliH"
                     /locus_tag="OO572_002186"
     CDS             7514..8200
                     /gene="fliH"
                     /locus_tag="OO572_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6661181.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8200..9573
                     /gene="fliI"
                     /locus_tag="OO572_002187"
     CDS             8200..9573
                     /gene="fliI"
                     /locus_tag="OO572_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6661182.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9595..9936
                     /gene="fliJ"
                     /locus_tag="OO572_002188"
                     /pseudo
     CDS             9595..9936
                     /gene="fliJ"
                     /locus_tag="OO572_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            9933..11060
                     /gene="fliK"
                     /locus_tag="OO572_002189"
     CDS             9933..11060
                     /gene="fliK"
                     /locus_tag="OO572_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6661183.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11165..11629
                     /gene="fliL"
                     /locus_tag="OO572_002190"
     CDS             11165..11629
                     /gene="fliL"
                     /locus_tag="OO572_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6661184.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11634..12638
                     /gene="fliM"
                     /locus_tag="OO572_002191"
     CDS             11634..12638
                     /gene="fliM"
                     /locus_tag="OO572_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6661185.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12635..13048
                     /gene="fliN"
                     /locus_tag="OO572_002192"
     CDS             12635..13048
                     /gene="fliN"
                     /locus_tag="OO572_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6661186.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13051..13416
                     /gene="fliO"
                     /locus_tag="OO572_002193"
     CDS             13051..13416
                     /gene="fliO"
                     /locus_tag="OO572_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6661187.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13416..14181
                     /gene="fliP"
                     /locus_tag="OO572_002194"
                     /pseudo
     CDS             13416..14181
                     /gene="fliP"
                     /locus_tag="OO572_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            14191..14460
                     /gene="fliQ"
                     /locus_tag="OO572_002195"
     CDS             14191..14460
                     /gene="fliQ"
                     /locus_tag="OO572_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6661188.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14469..15254
                     /gene="fliR"
                     /locus_tag="OO572_002196"
     CDS             14469..15254
                     /gene="fliR"
                     /locus_tag="OO572_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6661189.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15539..16163
                     /gene="rcsA"
                     /locus_tag="OO572_002197"
                     /pseudo
     CDS             15539..16163
                     /gene="rcsA"
                     /locus_tag="OO572_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(16207..16395)
                     /gene="dsrB"
                     /locus_tag="OO572_002198"
     CDS             complement(16207..16395)
                     /gene="dsrB"
                     /locus_tag="OO572_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6661190.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16558..16785
                     /gene="yodD"
                     /locus_tag="OO572_002199"
     CDS             16558..16785
                     /gene="yodD"
                     /locus_tag="OO572_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6661191.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17083..17898
                     /locus_tag="OO572_002200"
                     /pseudo
     CDS             17083..17898
                     /locus_tag="OO572_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(17895..19589)
                     /gene="yedQ"
                     /locus_tag="OO572_002201"
                     /pseudo
     CDS             complement(17895..19589)
                     /gene="yedQ"
                     /locus_tag="OO572_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19760..19942)
                     /locus_tag="OO572_002202"
     CDS             complement(19760..19942)
                     /locus_tag="OO572_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6661192.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20021..20938)
                     /locus_tag="OO572_002203"
     CDS             complement(20021..20938)
                     /locus_tag="OO572_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6661193.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21111..22031
                     /gene="yedA"
                     /locus_tag="OO572_002204"
     CDS             21111..22031
                     /gene="yedA"
                     /locus_tag="OO572_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6661194.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22020..22490)
                     /gene="vsr"
                     /locus_tag="OO572_002205"
     CDS             complement(22020..22490)
                     /gene="vsr"
                     /locus_tag="OO572_002205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6661195.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22471..23889)
                     /gene="dcm"
                     /locus_tag="OO572_002206"
     CDS             complement(22471..23889)
                     /gene="dcm"
                     /locus_tag="OO572_002206"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6661196.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23956..24496)
                     /locus_tag="OO572_002207"
                     /pseudo
     CDS             complement(23956..24496)
                     /locus_tag="OO572_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24536..>24771)
                     /locus_tag="OO572_002208"
     CDS             complement(24536..>24771)
                     /locus_tag="OO572_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661197.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         6251 a         6359 c         6111 g         6050 t
ORIGIN      
        1 tgaaaaaacg agccttatat ccccgccctg aaggacgggc ttttacggtg cactggataa
       61 gcctggagat tatctacacc tgcatggaca tcattgtact atgccccgta catgctccat
      121 cacatcctca acatcaaagt attagagcct tgcaaaccat tatgcgtctc agtttgtctc
      181 atctatctta cgcaaaaaaa atagaactaa tatcaagcgg cgctatattg cgtattaaat
      241 aaaaccacct caattacatt aataaatcta aataaatatc atgcaaataa tttccatggt
      301 tatttttagg ctatttaaat taaaaaaata aattatgagt caatcataaa taacaacctt
      361 taatataatt tgactaccaa atttatattc gttcacaaaa tagtcatgca ttgtgtgcaa
      421 tagaaacagt tcagataaaa atagggatct acttcacaat ttaaatgaga aactaaaact
      481 tacatcttga aataatctca ttgattggat gaatatttgt agtgcagtga catcattttt
      541 aaataatagt tcaaataaaa gggctcacga tgaaaaaatt aacagtggca atttctgctg
      601 tagctgcatc agtactgatg gcgatgtctg ctcaggcagc agagatttac aataaagaca
      661 gtaacaagct ggatctgtac ggaaaagtta atgctaagca ctacttctct tctaacgatg
      721 cagatgatgg cgacacaact tatgttcgtc tgggcttcaa aggcgaaact caaatcaacg
      781 atcaactgac tggtttcggt cagtgggaat atgaattcaa aggcaaccgt gctgaatctc
      841 aaggttcttc caaagataaa acccgtcttg ccttcgctgg cctgaaattc ggtgactacg
      901 gctccatcga ttatggccgt aactacggtg tagcatacga tatcggtgca tggaccgacg
      961 ttctgccaga attcggtggc gatacctgga cccaaacaga tgtgttcatg actggtcgca
     1021 ccacaggtgt tgcaacctat cgtaacaacg acttctttgg tctggttgat ggtctgaact
     1081 ttgctgctca gtatcagggt aaaaatgacc gcactgacgt aactgaagct aacggtgatg
     1141 gtttcggttt ctccactact tatgagtatg aaggattcgg tgtaggtgca acctatgcta
     1201 aatctgaccg cactaatgat caggttatct acggtaacaa cagcctgaat gcatctggtc
     1261 aaaatgctga agtatgggca gctggtctga aatatgatgc gaacaacatc tatctggcta
     1321 ccacctattc tgaaacccag aacatgactg tttttggtaa taaccatatt gccaacaaag
     1381 cacaaaactt cgaagtagtt gcacaatatc agttcgactt cggtctgcgt ccgtccgttg
     1441 cttacctgca atctaaagga aaagacttgg gtgcgtgggg tgatcaggac ctgattgaat
     1501 atattgatgt aggtgcaacc tattacttca acaaaaatat gtccactttt gttgattaca
     1561 aaatcaacct gattgataag agcgatttca cgaaagcatc tggcgttgct accgatgata
     1621 tcgttgctgt aggattggtt taccagttct aatctgatta ctcaaagata tgttgtggga
     1681 ggctttgcct ccccaacata taagtggcgc tctcaagcca ctttccttag aagcactatc
     1741 ttgcttctga ctatataaat taccctttat tttctgggac gtttcaacgc cccattttta
     1801 ataatactca gtgaacaatt aacgtatcaa ttagatttaa taacaacgat atccgtacct
     1861 aacctgatat ctaatgtcat aagcatccct ttaattatcc cctcaacctc ttgtaaattt
     1921 cccactcttg agctccacat agcccaaacg ccggatgcaa aaaaaatcat acgtgcatct
     1981 ctgaaattgt gatggaggaa gtgagtatca attatatgtg cttataaacg ttcccgattg
     2041 gcacagaagt caccggattg ttcaggctcc aataggttga gtatgtcgta tttattttcc
     2101 aaaataaaaa tcgatgctgt tgctccttaa taaaatactt ttactctctg gacgcttttc
     2161 ttagctctct tggtgttata ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac
     2221 acttgtaacc attttccctg gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag
     2281 gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttat tccctcgctt
     2341 gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct
     2401 gtccattttg ttctaggttc ggatagcatt aggttataac aacgacttat taatgattgt
     2461 ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc
     2521 attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc
     2581 tcccaacaat atattaactc ggggttctga ggtaagcaga aacctgtcgt gttacgaatt
     2641 tgatcagtta tcgcataaaa tttttgaaac ttttcaatta gatcaatggg ataataaagc
     2701 atttctgcaa gataaagacc tgcttctgga taattcacaa tataaaattc atatccacag
     2761 ggaaataata ttatctgatt attatcaaca gttagactat gcgtctccca attgataact
     2821 ttttttccct gacggatacg acataaagct ggcataagag gcttaaccct gtgaatctca
     2881 tgatgtttat gcatccgtat ttcttcaatc tttaggttag acttatctct tgccagcata
     2941 ctcttaccct attttatctc ataaactggt gttatctcag cggttgcaat tttattggca
     3001 ttaagcatat aaccaactaa cgctccgctg gaattagaat ccacaggaat cttatcagtt
     3061 tttagagccc atactttaaa ctggtaatga tggggtttat ctcctttggg aggacatgct
     3121 ccaccaaatc ctgcataacc aaaatcattt cggccttgaa cagcaccagt cggaagtttt
     3181 gtaccatcac gtcttcctgc atcagtgggc aaatatgtta cagttgctgg aatattagca
     3241 acagtccagt gccaccatcc actgcctgta ggagcatctg gatcatacac agttacggca
     3301 aagcttttgg tgccttcagg agcaccagac caggttaatg agggcgatgt attaccacct
     3361 tcacacccaa atccagaaaa tacatgagac gttgtaagtt gctctcctgt ttttatttca
     3421 ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc caatacactt
     3481 gaaacgataa gttttttcat aaaaacctca ttgttgtgac ctatcgttat tttatttgat
     3541 gtgccttgat ctcattatgc ataaaggcgc aatgatcgcg caaatgcaat cataattata
     3601 aaggtgcccc gaacattagc cataatatgc acaaatcaga ttcctgttat ctcaaggaga
     3661 attatttttt aatgcggtgc acttcgtgac aacaagttaa tgactaacac accggcacaa
     3721 atcaacatca tgcctatgat agctggcagg tccagtcgtt gaccaaaaaa tccccaggaa
     3781 agcaaactaa tcagaacgat accgactcct gaccagatag cataagcaat ccctgtaggt
     3841 atataagcca gcgtctgagc taataaccag aatgatgcac aatagcaaat aattgtgcca
     3901 acagagggcc ataaccgtgt aaacccctct gaaaacttca ttaaggttgt accaatgacc
     3961 tctgcaagta ttgcgccacc aagataaata taagggttca tagcatattc tttcctgttc
     4021 atactggaga gaattgtact acagtttgaa cacaactcac cgttttcatc ctggtgtatg
     4081 tgtatccaag gggctctggc gtggcaagag ataaaggcag gaacaataat gatcagaagg
     4141 aaccccaggc taacgaatga gttcactctt cctgggagca aagcgaagac aaaacatgtt
     4201 atcgcgttta cggatcgtgg atggcagaaa acaaccagga tcaggtatcc attttaaacc
     4261 agaaattgag tgagtttgcc ccatccatgc cccacgctgt gggatcggat ataattaaac
     4321 aagcatagtc aacaggttac acaacctaca cctgcatgga catcacttcc tgatacgccg
     4381 ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat
     4441 cggtcatcac atcgtttagc gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg
     4501 tgcgggcgac tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc tgcccggcaa
     4561 aactaatggt cggttgcggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta
     4621 actggctgat aaccccttca atcccctgta tcgctgacat tttcgtctcc cggataattt
     4681 ctggtaacaa agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa
     4741 ctggtttatt ggcggataga aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga
     4801 gtgtgctgac ccgagagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc
     4861 cacgaagtgc gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg
     4921 cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt tccgcggcag tggcggtcat
     4981 ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc
     5041 cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt
     5101 cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa gttcacgaac tgcgcctggc
     5161 gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa ctgctcgatc aggaaaagtt
     5221 tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag gcgagctttc
     5281 tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg caatgccgaa
     5341 accgtcttta ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg taaatctgtt
     5401 acccggccgc gcactcgatg aagggcaaat tagcgctatt gtacatctgg tttccagcgc
     5461 ggttgctggt ctgccgcctg gaaacgtcac gctggtggat cagggcgggc atctgttaac
     5521 ccagtccaat accagcgggc gcgatcttaa tgacgctcag ttgaaatatg ccagcgatgt
     5581 cgaaggccgt attcagcagc gtattgaagc gatcctgtca cctattgttg gtaacggtaa
     5641 tattcacgcc taggtcacgg cgcaactgga cttcgccagt aaagaacaaa cggaagaaca
     5701 gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt tcacgccagc ttaatgagag
     5761 cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg gcgttgtcga atcaaccggc
     5821 acctgcgaat aacgcgccaa tcagcacgcc tccgacaaat caaaataacc gccagcagca
     5881 ggcgagcacc accagcaata gtgggccgcg tagcacacag cggaatgaaa ccagtaacta
     5941 cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc aacgtctgtc
     6001 agtcgcggta gtggtgaatt acaaaacctt gccagacggt aaaccattgc ctctcagcaa
     6061 cgaacagatg aagcaaattg aagctctgac ccgcgaggcg atgggctttt ctgaaaaacg
     6121 cggtgattcg ctcaatgttg ttaactcgcc gttcaatagc agtgacgaaa gcggcggagc
     6181 actgccgttt tggcaacagc aagtgtttat cgatcagttg ctggctgccg gtcgctggtt
     6241 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     6301 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     6361 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     6421 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     6481 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     6541 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     6601 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     6661 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     6721 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     6781 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     6841 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     6901 catccgcaaa ttatcgccac cattctggtg catctgaggc gcgcccaggc cgccgatatt
     6961 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     7021 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     7081 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     7141 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     7201 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt cgatgatcgc
     7261 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     7321 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     7381 ctgcgcgacg atctcgccaa gcgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     7441 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     7501 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     7561 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     7621 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccacg agcagggtta
     7681 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     7741 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     7801 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     7861 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     7921 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     7981 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     8041 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     8101 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     8161 gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc
     8221 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     8281 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     8341 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     8401 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     8461 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     8521 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     8581 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     8641 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     8701 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     8761 aaaagtgtgc tgctggggat gatggcacgc tacacccgcg ccgatgtcat tgtcgtgggt
     8821 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     8881 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     8941 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     9001 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     9061 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     9121 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     9181 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     9241 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     9301 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     9361 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     9421 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     9481 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     9541 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     9601 gaacagctca aaatgttgat cgattatcag aatgaatatc gcaataacct caatagcgat
     9661 atgagtgccg ggatgaccag caaccgctgg atcaactatc agcagtttat ccagacgctg
     9721 gaaaaagcca ttactcagca tcgccagcaa cttaatcagt ggacgcagaa agttgacatt
     9781 gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg cctggcagac actgcaggaa
     9841 cggcaatcca cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag
     9901 ttcgcccagc gcgccgccat gaggaaacct gaatgattcg cttagcgccc ttgattaccg
     9961 ccgacgttga caccaccaca ttgcctggcg gcaaagccag cgatgctgca caagattttc
    10021 tcacgttgtt gagcgaagca ttagcaggcg agacaacaac tgacaaagcg gccccccagt
    10081 tgctggtagc aacagataag cccacgacaa aaggcgagcc gctggtcagc gatattcttg
    10141 ccgatgcgca acaagcggat ttactgatcc ctgtggatga aacacttcct gtcatcaacg
    10201 acgaacaatc cacatcaaca ccgttaacca ccgctcagac gatgacgttg gctgcggtgg
    10261 ctgacaaaaa tacgacaaaa gacgaaaaag cggatgatct taatgaagac gtcaccgcaa
    10321 gtctgagcgc cctttttgcg atgttgccgg gttttgacaa tacgcccaaa gtgactgatg
    10381 caccgtcaac cgtgttaccg gcagagaaac caacgctctt cacaaaactg acttctgcgc
    10441 aactcactac agcacagcct gatgacgccc ccggcacacc agctcagcca ttaacaccgc
    10501 tggtagcaga agcccagagt aaagcggaag tcatcagcac gccttcgccg gtgaccgctg
    10561 acgccagccc gctaatcact ccacaccaga cacagccact gcccaccgtc gccgcgcctg
    10621 ttttgagtgc accgctgggt tctcacgaat ggcaacaatc attgagccag catatttcgc
    10681 tgttcacccg ccaggggcaa caaagtgcag agttgcgtct gcacccacag gatttaggtg
    10741 aagtgcaaat ttccctcaaa gtggatgata accaggcgca gatccagatg atttcaccgc
    10801 atcagcacgt acgcgccgcc ctggaagcag cgctgccggt actgcgtacg cagctggccg
    10861 aaagtggcat tcagttaggg caaagcaaca ttagtggcga aagctttagt ggtcagcagc
    10921 aggccgcttc ccagcaacag caaagccaac gcacagcaaa ccatgaacct ctggcggggg
    10981 aagacgacga tacgcttccg gttcccgtct ctttacaagg gcgtgtaaca ggtaacagcg
    11041 gcgttgatat tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt
    11101 tgttgcgctc aagacgcagg ataattagcc gataagcagt agcgacacag gaagaccgca
    11161 acacatgact gattacgcga taagcaagaa aagcaagcga tcgctttgga tcccgattct
    11221 ggtattcatt accctcgcgg cctgtgccag cgcaggttac agctactggc attcgcatca
    11281 ggttgccgct gacgacaaag cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct
    11341 ggataccttt acggtcaatt tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct
    11401 gcgactgaaa gatgaagcaa cccgctcgcg gctgagtgag tatttgccgg aagtccgtag
    11461 tcgcttgctg ttactgtttt cacgtcagga tgctgccgta ctggcgacag aagaaggcaa
    11521 gaaaaacctg attgccgaga ttaaaaccac actttccacc ccgcttgttg ccgggcaacc
    11581 gaaacaggat gtcaccgacg tgctgtatac cgcttttatt ctgcgataac gacatgggcg
    11641 atagtattct ttctcaagct gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag
    11701 acgaaccgac agccagtatt agcggcgaaa gtgacattcg tccgtacgat ccgaataccc
    11761 aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag cgctttgccc
    11821 gccattttcg tatggggctg ttcaacctgc tgcgccgtag cccggacatt accgtcgggg
    11881 ccatccgcat tcagccgtac catgaatttg cccgcaacct gccggtgccg accaacctga
    11941 acctaatcca cctgaaaccg ctacgcggca cagggctggt agtgttctcg ccgagtctgg
    12001 tgtttatcgc cgtggataac ctgtttggcg gcgatggacg cttcccgacc aaagtggaag
    12061 gtcgcgagtt tacccatact gaacagcgcg tcatcaaccg tatgttgaag ctggcgctgg
    12121 aaggctatag cgacgcctgg aaagcgatta atccgctgga agttgagtac gtgcgttcgg
    12181 aaatgcaggt ggaatttacc aatatcacca cctcgccgaa cgacattgtg gttaacacgc
    12241 cgttccatgt ggagattggc aacctgaccg gcgaatttaa tatctgcctg ccattcagca
    12301 tgatcgagcc gctacgggaa ttgttggtta acccgccgct ggaaaactcg cgtaatgaag
    12361 atcagaactg gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg gaactggtgg
    12421 ccaactttgc cgatatctcg ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg
    12481 tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt tgacggcgtc ccggttctga
    12541 ccagtcagta cggtactctc aacggtcagt atgcgttacg gatagaacat ttgattaacc
    12601 cgattttaaa ttctctgaac gaggaacagc ccaaatgagt gacatgaata atccggccga
    12661 tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgagc aaaaatcaac
    12721 cagcgaaaaa agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg
    12781 aacgttgcag gatatcgacc tgattatgga tattccggtc aagctgaccg tcgagctggg
    12841 ccgtacgcgg atgaccatca aagagctgtt gcgtctgacg caagggtcag tcgtggcgct
    12901 ggacggtctg gcgggcgaac cgctggatat tctgatcaac ggttatttaa tcgcccaggg
    12961 cgaagtggtg gttgttgccg ataaatatgg cgtgcggatc accgatatca ttaccccgtc
    13021 cgagcggatg cgccgcctga gccgttagtg atgaataacc atgctactgt gcaatcttcc
    13081 gcgccggttt ctgctgagcc actgctgcag gtgagcggcg cactcatcgc cattattgcc
    13141 ctgatcctcg ctgctgcctg gctggtaaaa cggctgggat ttgcccctaa acgcactggc
    13201 gttaacggtc tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg ggtcgtggtg
    13261 gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg cagggcaaat caatctgctg
    13321 cataaacttc ccccttcagc accaacggaa gagataccgc agaccgattt tcagtcggtc
    13381 atgaaaaatt tgcttaagcg tggcgggaga tcctgatgcg tcgtttgttt tctgtcgcac
    13441 cggtccttct ctggctgatt acgcccctcg ccttcgcgca actgccgggt atcaccagcc
    13501 agccgctacc tggcgggtat caccagccag ccgctacctg gcggtggaca aagctggtcg
    13561 ctcccggtgc agacgctggt gttcatcacc tcgttgacgt tcattccagc gattttactg
    13621 atgatgacca gtttcacccg catcattatt gtttttggtt tattgcgtaa cgcgctggga
    13681 acgccctccg cgccacctaa ccaggtattg ctggggctgg cactgttttt gacctttttt
    13741 attatgtcgc cagtgatcga caaaatttat gtcgatgcgt accagccatt cagcgaagag
    13801 aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
    13861 cgtcagaccc gcgaggcaga tttagggttg tttgccagac tggcgaatac cggcccgttg
    13921 caggggcctg aagctgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg
    13981 aaaaccgcat ttcagatagg cttcacgatt ttcatccctt ttttgattat cgacctggtg
    14041 attgccagcg tgttgatggc attggggatg atgatggttc cccccgccac cattgctctg
    14101 ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg
    14161 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatggga
    14221 actgaagcga tgaaagtcgc gctggcactg gcagccccgc tgttgctggt agcgctggtc
    14281 acgggcctta tcatcagtat tttgcaggca gccacgcaga ttaacgaaat gacgctgtcg
    14341 tttattccga aaatcatcgc cgtatttatc gccatcatta ttgccggacc gtggatgctc
    14401 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    14461 cccgtacaat gatgcaggag acaagcgatc aatggctttc ctggttaagc ctgtacttct
    14521 ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc gaacgcagcg
    14581 tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgccatt gccccatcat
    14641 tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc gtacagcaga
    14701 tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgct gtgcgaaccg
    14761 ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacatttgtc gatcctggca
    14821 gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta ctgctgttcc
    14881 tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc
    14941 cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa gcagggagtt
    15001 tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg ctgaccctga
    15061 atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt gttattggat
    15121 ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg ttaattgcgc
    15181 ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt attagtgaat
    15241 taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc cgaaaaataa
    15301 tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa gctcactcac
    15361 atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt tacgggatag
    15421 taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg ttccgtttag
    15481 tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag ggtatgccat
    15541 gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg ggtatctgtt
    15601 gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg accttgccat
    15661 agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt tcatccacga
    15721 tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata cgttatttat
    15781 cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca gaaaaaaatt
    15841 tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc
    15901 tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa
    15961 ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc
    16021 aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca
    16081 aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg
    16141 gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga
    16201 ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt cattaaagaa
    16261 ccagatgccg agcgggtagt cttccagcgc aaccaggtac attgtgcctt cactaaactc
    16321 ctcaactgcc agtaccactc caggacgacg cggaccgcca tccgttttga ctgttacccg
    16381 atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt
    16441 ttctggcagc gccaagcgcg cgcgagtgct gattttctcg acggtctata cttaagagat
    16501 gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg
    16561 aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc
    16621 ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct
    16681 gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc
    16741 gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa
    16801 atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc
    16861 gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga
    16921 aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc
    16981 ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg
    17041 ctgttttgag aatcatcctg gtaaggggag tactgcgcga ccatgttttc aattcaacaa
    17101 ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg
    17161 caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt
    17221 agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta
    17281 ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac
    17341 ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg
    17401 ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt
    17461 gccgaatgga cgggattaag ccgtagctag gcggcgctga cgcagctgca tgaggcgtcg
    17521 gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac
    17581 gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc
    17641 ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcagacgc
    17701 ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat
    17761 tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga tgaggatccg
    17821 gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat
    17881 tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc ggcctgtttt
    17941 gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt
    18001 tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc
    18061 agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc
    18121 agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc
    18181 tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc
    18241 tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa
    18301 aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacagtgcg
    18361 ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc
    18421 agaacataca tgttgctgac catccggcga atcacatacc aggagaggag taacatggtg
    18481 gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg
    18541 ccttcgctta gcgtatggac gcgcgccagc acaccgtcga aatggtccag acgttcccag
    18601 ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc
    18661 agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag
    18721 gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg
    18781 gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac
    18841 cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg
    18901 tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg
    18961 ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac
    19021 aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc ctgttcaacc
    19081 atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt
    19141 tcattgtcga ggctttcatt ttcgcgagac aggaaattcc cctcgctgac taatgcatcc
    19201 gatacgccat tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg
    19261 tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc
    19321 acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa
    19381 tgcttatcga gcgcgttggc tacgttttcc agatgattac gctagctgga gatataggca
    19441 tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata
    19501 aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc
    19561 tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat
    19621 tctggcataa cccggcaata gcgtaccgca aaacagcgat aattttataa tggtggcatg
    19681 ataagacgcg actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc
    19741 tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa
    19801 gatcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa
    19861 ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt
    19921 cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta
    19981 gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca
    20041 ttttcgctac tgctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
    20101 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgatatgtt
    20161 caatagcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
    20221 tcgccagcgt gccgacaatc gataatgctt tcatcagcca aggcgcgata atcaataacc
    20281 ctttacctaa tgcctgcatc agcgcgctag atttttccgc cagccaatac cctaggtcat
    20341 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
    20401 ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg
    20461 cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt
    20521 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgctggat
    20581 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga
    20641 gaaacgcgcc accaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga
    20701 tcagcgccag cggcaccaga atcactttat tgatcagcga tcctttcgcc acgccccaga
    20761 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataaat
    20821 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcaca gagatatcgt
    20881 ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
    20941 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
    21001 gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat
    21061 gattttcacg tttactatga accacctttt tatttccgcc gtgagggatt atgcgtttcc
    21121 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
    21181 attttgtcat tcggattggc gtgggaagct ggcctccgtt aatgatggcg ggcgttcgat
    21241 tccaggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc
    21301 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
    21361 gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtgattg
    21421 caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa acgcgcaaac
    21481 tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
    21541 atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
    21601 ttggctcagt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg
    21661 agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt gaaaaactga
    21721 cggcgctccc ttccctgtca ggcttccttg cggtcggcta tctggcgctg tttggttcga
    21781 ttatcgccat taacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca
    21841 gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa
    21901 cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca
    21961 cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat
    22021 caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc
    22081 cactcttcca gacgctcggt aagcgccgca tccgtcagct tctcgcgcct acgtaacgca
    22141 cactcccaga caatcagtac gcgccagccg agctcctgca agcgactgat atcgcggcga
    22201 tcgcgttcaa catttttacc tatcttctcc agccagaact cagttcgagt cgcaggcact
    22261 ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga
    22321 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca
    22381 aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca
    22441 atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc
    22501 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
    22561 aaagaccggc acgaccaccg agttgccgaa ctggcgatag gcctgagtgt ccgaaaccgg
    22621 aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg
    22681 cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt
    22741 ggccatatcc caaccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgagcaga
    22801 cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
    22861 accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
    22921 gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg
    22981 gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa
    23041 acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat cgatgatttt
    23101 cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca tagcccagtt cgtccagcgt
    23161 ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacgttttc
    23221 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
    23281 gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
    23341 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
    23401 ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat
    23461 gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata
    23521 agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccac caatcgattc
    23581 aaaaccacga cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg
    23641 cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact
    23701 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
    23761 attctcaccc acaccattaa gctgcgccac cagcatttta acatcataaa tttgcagcag
    23821 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
    23881 ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg
    23941 tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc
    24001 ttcattctcc cccgccagtt cggcaccgag cttcgccatc aaacagcgcc aagctcaatg
    24061 cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg
    24121 catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag
    24181 cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcac
    24241 ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat
    24301 ttttcgccag gctcacgata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga
    24361 catcgtcatc tgccgccagt ttctgtgccg tagcccagac acggcgaaaa tgacacaggt
    24421 catgcgccgc gtcctgatgt tgatgatggt ttttcaacca gttctcgaac tatgcctgcc
    24481 agtgttgtaa ctccatacgc gctccgttgc caaagagtct caaccttagc agtttttatt
    24541 catagcgtct gcaacgtgcg gaacgaggtc gggtgtacca tgcaatgccg cctaacaagg
    24601 caccaaccag ggccataaac agaaatccga ccaatgctcc taaccacttc cccgcggtag
    24661 atcctaaatc actttcaaat cccgacacgg gggcatcggg gatccggctc atcacccaga
    24721 tgtatcgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc c
//
LOCUS       DAJTYL010000057        24608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYL010000057 DAJTYL010000000
VERSION     DAJTYL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.057"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..796
                     /gene="proB"
                     /locus_tag="OO572_002209"
     CDS             <1..796
                     /gene="proB"
                     /locus_tag="OO572_002209"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6661198.1"
                     /translation="TRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVG
                     DNDNLSALAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDS
                     VSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLE
                     NRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGR
                     DIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            808..2061
                     /gene="proA"
                     /locus_tag="OO572_002210"
     CDS             808..2061
                     /gene="proA"
                     /locus_tag="OO572_002210"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6661199.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2176..2251
                     /locus_tag="OO572_002211"
     tRNA            2176..2251
                     /locus_tag="OO572_002211"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2209..2211,aa:Thr,seq:cgt)
     gene            complement(2266..3426)
                     /locus_tag="OO572_002212"
     CDS             complement(2266..3426)
                     /locus_tag="OO572_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6661200.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3318..3650)
                     /locus_tag="OO572_002213"
                     /pseudo
     CDS             complement(3318..3650)
                     /locus_tag="OO572_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3737..4081)
                     /locus_tag="OO572_002214"
     CDS             complement(3737..4081)
                     /locus_tag="OO572_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661201.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4182..4361)
                     /locus_tag="OO572_002215"
     CDS             complement(4182..4361)
                     /locus_tag="OO572_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR6661202.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4458..5387)
                     /locus_tag="OO572_002216"
     CDS             complement(4458..5387)
                     /locus_tag="OO572_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6661203.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5390..5581)
                     /locus_tag="OO572_002217"
     CDS             complement(5390..5581)
                     /locus_tag="OO572_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661204.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(5583..6212)
                     /locus_tag="OO572_002218"
     CDS             complement(5583..6212)
                     /locus_tag="OO572_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6661205.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6209..6784)
                     /locus_tag="OO572_002219"
     CDS             complement(6209..6784)
                     /locus_tag="OO572_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HCR6661206.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(6781..6945)
                     /locus_tag="OO572_002220"
     CDS             complement(6781..6945)
                     /locus_tag="OO572_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6661207.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(6956..7249)
                     /locus_tag="OO572_002221"
     CDS             complement(6956..7249)
                     /locus_tag="OO572_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR6661208.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7273..7656)
                     /locus_tag="OO572_002222"
     CDS             complement(7273..7656)
                     /locus_tag="OO572_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661209.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(7656..8261)
                     /locus_tag="OO572_002223"
     CDS             complement(7656..8261)
                     /locus_tag="OO572_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HCR6661210.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8363..8521)
                     /locus_tag="OO572_002224"
     CDS             complement(8363..8521)
                     /locus_tag="OO572_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661211.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTETHVH
                     AESSLVSP"
     gene            complement(8518..8670)
                     /gene="kil"
                     /locus_tag="OO572_002225"
     CDS             complement(8518..8670)
                     /gene="kil"
                     /locus_tag="OO572_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR6661212.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(8655..8789)
                     /locus_tag="OO572_002226"
     CDS             complement(8655..8789)
                     /locus_tag="OO572_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661213.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQPEALCAMK
                     "
     gene            complement(8984..9454)
                     /locus_tag="OO572_002227"
     CDS             complement(8984..9454)
                     /locus_tag="OO572_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661214.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9463..9789)
                     /locus_tag="OO572_002228"
     CDS             complement(9463..9789)
                     /locus_tag="OO572_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR6661215.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10153..10557)
                     /locus_tag="OO572_002229"
                     /pseudo
     CDS             complement(10153..10557)
                     /locus_tag="OO572_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10554..11186)
                     /locus_tag="OO572_002230"
     CDS             complement(10554..11186)
                     /locus_tag="OO572_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6661216.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11290..11505
                     /locus_tag="OO572_002231"
     CDS             11290..11505
                     /locus_tag="OO572_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6661217.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            11622..11903
                     /locus_tag="OO572_002232"
     CDS             11622..11903
                     /locus_tag="OO572_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661218.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            11938..12099
                     /locus_tag="OO572_002233"
     CDS             11938..12099
                     /locus_tag="OO572_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661219.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12086..12916
                     /locus_tag="OO572_002234"
     CDS             12086..12916
                     /locus_tag="OO572_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR6661220.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            12913..14289
                     /locus_tag="OO572_002235"
     CDS             12913..14289
                     /locus_tag="OO572_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6661221.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14362..14688
                     /locus_tag="OO572_002236"
     CDS             14362..14688
                     /locus_tag="OO572_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661222.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            14685..14885
                     /locus_tag="OO572_002237"
     CDS             14685..14885
                     /locus_tag="OO572_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661223.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            14897..15085
                     /locus_tag="OO572_002238"
     CDS             14897..15085
                     /locus_tag="OO572_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661224.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15088..15252
                     /locus_tag="OO572_002239"
     CDS             15088..15252
                     /locus_tag="OO572_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661225.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15233..15673
                     /locus_tag="OO572_002240"
     CDS             15233..15673
                     /locus_tag="OO572_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6661226.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            15670..16260
                     /locus_tag="OO572_002241"
     CDS             15670..16260
                     /locus_tag="OO572_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine-binding protein"
                     /protein_id="HCR6661227.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16257..16430
                     /locus_tag="OO572_002242"
     CDS             16257..16430
                     /locus_tag="OO572_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HCR6661228.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16397..16572
                     /locus_tag="OO572_002243"
                     /pseudo
     CDS             16397..16572
                     /locus_tag="OO572_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            16575..16976
                     /locus_tag="OO572_002244"
     CDS             16575..16976
                     /locus_tag="OO572_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661229.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            16936..17166
                     /locus_tag="OO572_002245"
     CDS             16936..17166
                     /locus_tag="OO572_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR6661230.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17138..17749
                     /locus_tag="OO572_002246"
     CDS             17138..17749
                     /locus_tag="OO572_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR6661231.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            17746..17952
                     /locus_tag="OO572_002247"
     CDS             17746..17952
                     /locus_tag="OO572_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR6661232.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            17930..18595
                     /locus_tag="OO572_002248"
     CDS             17930..18595
                     /locus_tag="OO572_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6661233.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            18592..19215
                     /locus_tag="OO572_002249"
     CDS             18592..19215
                     /locus_tag="OO572_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6661234.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            19648..19971
                     /locus_tag="OO572_002250"
     CDS             19648..19971
                     /locus_tag="OO572_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6661235.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            19955..20431
                     /locus_tag="OO572_002251"
     CDS             19955..20431
                     /locus_tag="OO572_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6661236.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20428..20865
                     /locus_tag="OO572_002252"
     CDS             20428..20865
                     /locus_tag="OO572_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6661237.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21070..21612
                     /locus_tag="OO572_002253"
     CDS             21070..21612
                     /locus_tag="OO572_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6661238.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSYTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            21832..22047
                     /locus_tag="OO572_002254"
     CDS             21832..22047
                     /locus_tag="OO572_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6661239.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22195..22437
                     /locus_tag="OO572_002255"
     CDS             22195..22437
                     /locus_tag="OO572_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR6661240.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22473..22961
                     /locus_tag="OO572_002256"
     CDS             22473..22961
                     /locus_tag="OO572_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6661241.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            22939..24438
                     /locus_tag="OO572_002257"
     CDS             22939..24438
                     /locus_tag="OO572_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6661242.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            24439..>24608
                     /locus_tag="OO572_002258"
     CDS             24439..>24608
                     /locus_tag="OO572_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661243.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQF"
BASE COUNT         6600 a         5786 c         6014 g         6208 t
ORIGIN      
        1 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
       61 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      121 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      181 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      241 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      301 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      361 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      421 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      481 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
      541 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
      601 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
      661 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
      721 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
      781 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
      841 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
      901 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
      961 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1021 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1081 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1141 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1201 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1261 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1321 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1381 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1441 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1501 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     1561 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     1621 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     1681 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     1741 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     1801 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     1861 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     1921 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     1981 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2041 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2101 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2161 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2221 gtcgtaggtt cgactcctat tatcggcacc atttaaatca ataagttacc tcgcatttaa
     2281 gtaaaccacg ttctcctctt gtgccgtatt tgtgccattg cgacttataa tcgcatcgat
     2341 tttgcttgcg tgctcggtga gatgcccggc tgaaaggtgg gcgtatcttt gaaccatttc
     2401 gagagtttcc catcctccca tctctttaag tgcaagaaga gagacaccgg actgaaccag
     2461 ccagcttgcc caggtatgcc tcagatcatg gaagcggaag ttgctaatgc ctgcccgctt
     2521 taacgctccc ttccatgcct tgttgctgtc ggttctcatc ttccttaccg ctgctgtttt
     2581 tgttccgtcg cttcggtagg caggtttggt gtggacaaat acccatctct tatggagccc
     2641 ctgctgtttt cttaatatct ggcatgcggt ttcgttaaga ggaactccga tcgcattgcc
     2701 agcttttgtt tcatcagggt gcatccatgc cattttctta tccagatcga cctgtgacca
     2761 ctcaaggtct gtaacgttgg aacggcgaag gcctgtcgtg attgcaaaca tgaccacagg
     2821 gaagaagtga ggagcaattt ctgcaaacag gcgcttcgat tcctcctctg taagccatct
     2881 gatgcgtcca ttcttaacgc gtggtgttga tattttgggt gccctgtcaa gccatcccca
     2941 ttcaacagcc atattgagaa tggcgcgaag tattgccaga tgcctcgtct tcgttccttt
     3001 gcttgcaagc tttggtttat actccggcac cggattgcca agtcgcaaac acctgtcccg
     3061 gctcatctcc cagttcaggc gatggcggcg gttttccatc ccgtctaccg cctccattat
     3121 tttttctgtt gttatgtcag agagaatggc ttctctgaag tgcaacatcc agaacgatat
     3181 aatgctcttg tcatcatcaa tggacttctt atccgatttc tcacgcagcc accgtatgca
     3241 ggcttccttg aatagctttt tcggtgattc cccgagattt tttactctcc acgcttctgc
     3301 tttcagacga tcgtgaagtt cttgcgcttg ccttttgtcc gatgtttcaa gagagcgtct
     3361 aactcttgat ccatctggcg cgacgaaatc gcagtgccac gtgccaccgc gtagtttgat
     3421 tgacatgctt taacctcctg cacatcaacc gcattcaccg cgctattgtg tctcacagac
     3481 ttaagcgccg caatgcagtc tgacttgcaa atgcgatatg ggcttttagg tttatctgga
     3541 tttatctttg cggcctgaag tcgtccactt cgtatccact gcgtgatagt gcctttgtct
     3601 accttcagat acgacgctgc ctcttcacga gtgaagattt cttcttccac ctggaatctc
     3661 catttattgg attggtatta ttgcggtagg tctggatatc attgagcaat gaacaggcct
     3721 catcgagtgt gaggcgttag tccttgcgta gctcgctgat tcttctgtaa gtctctggtg
     3781 ctttgttccc gtacgtcttc atttcagact tcaacagagc aacgagtgaa tcccattcgt
     3841 tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gtcgaatgaa tctctgattt
     3901 ctggaatctg ctcaacaagt gcaacgcatc gccggaaatc ggctgcgtca tgtggagcac
     3961 cgaagctatg accatagata ttctttttca gtccacatgc gattgaggca agagttgcgc
     4021 tactgatgcc gacatcgtca gtcgattgcc atttcaaaac cttcatagcc aaatctgaca
     4081 tttcttgtct ccaataaaaa accgccatca ggcggcttgg tgttctttca attcttcaat
     4141 tcgaatattg gttacattgt tttcatatat gaataaataa attagctttt ttcgttgcct
     4201 tcgcgttctt tattaatttt gacaaactcg tttttaccac gctctccaaa tgcgtcttta
     4261 gagtcgttgt atccgcaatc gcagcacaca taatcaccag accatccacg cattgttttt
     4321 tcttttgcaa tatttccaga accgcatttt ggacaagaca tatcactacc tccaaagcat
     4381 gagtgagatg acaacgtaac attgattgga gattaacaat agattgctga tgtaaaagat
     4441 atgtataagc ttcgctttca aagtggaggc tctggtagcg gcatccagtg ggttacattg
     4501 ctaatcagac catattcatt agtttgagga tggttgccgt tatcgtctcc gtatttaaga
     4561 ctctccataa aaccataatg cttattccca ttaacgctca caaagccgta ataagctggt
     4621 ataacgccga tctcacacgt aatcagcaaa ggaaaactag ttctccaatt tagctcgccg
     4681 attacaggca ttctctcact acagcttatc caaccatccg gagttaccgg aacttgcgga
     4741 atggctgtct gctctcgaac gtcattaggc gcgataggtt ctgctgccaa ctgactggca
     4801 tatttgttaa tggtaacgat aagctcttgc tcagcctcat ccagacaatc accgatacct
     4861 cgcctgtcac cgtcaaaatc atcgaaatcg gcacgaatct tggcaacctt ctggattgcg
     4921 gacaacacct cactaggaat taccggatag ttggttgacg tttccgcgat ttcacgaaaa
     4981 ttattggttg acgaattctt gttttcccga aagtttccgg actgaagcat ggctgcgcga
     5041 taggcgttcc agccgacagc ttttccgtgt tcaaacgcgc tgtcaaagtc atcatccatt
     5101 tccatcgcat caggcacaga taccgtctct ggaggggcgg cgtaaagcgg agttacggta
     5161 atttcgtact cgtttatatc ctcgtcgctg atatttgcga aatagcgaag gatgcctttc
     5221 tctccacgac tacgagcgtc agagtcctga atgatatacg ccacaggctc tgcttccagc
     5281 gatgccagcg caattcgtgc cagttccatt tgttcgccac gagtaagccc gttttcaagc
     5341 ggatttttaa tgaacaattc aatacgttct ttggtaatag tggtcatagc tatttcacct
     5401 taatctcaat atttcgcagc tttagctcta ctggcaggtc tgactttcct gttaacgcta
     5461 atgcgagatt ttctggagta atgagagcag ttattgtttt ccccatcgcc agacgaataa
     5521 tcagtcgtat ctcgcgatcg tcacatgctc ctggtcgaac aattgatatt tgtccgtcca
     5581 tctcactctc ctttgatgcg aatgccagcg gcgcggattg cagcgatgac ctcagaaact
     5641 ttgtatgcca ttaccgtttg gtaatcatcg tgaaaatctg ttcgatgaag catgctgcta
     5701 cgttccggga gcagtattcc ccgctccccc agttctgcta tgcgattctc tgcggattcc
     5761 agcgccgcaa ccaattcgtc tacagttccg gcagcttgca gtgcgtaatc ggtaatagcc
     5821 atctcatgat caatttcagt accgttctca ttcgttgagg tgatagcaaa ataatcagag
     5881 tcgatttcgt tatcagctaa gtggcgtagc gtatcggcaa caagccggcc gttttcgatt
     5941 agcagcttcc ctaccgttag cgcaatatcc tcgttctcct ggtcgcggga tttgatgtat
     6001 tgctgattcc ttacccgttc atccagtagt gccagcacgg tttctggtcc ggccagaaat
     6061 ttgaaggcgt tgagcgcatc aatatccaca ccgtaatctt taagttcctg ttcacttaac
     6121 aaatcatcag ctggcaacat taacaggcgt tccattgctg gaattgcacg ttccgccgcc
     6181 tcacgcagtg cctgatagtt aatttcgctc actggccgcc tcctttgcga agctgggcgg
     6241 cgaactcgtt agccatcgct gaatatgggt gctcatgacc gccaacaacc ttcatgctgg
     6301 cagcaaacat ctccacgccc tgcgcccgca cttcatccag gaaagtgtcg gtggctgggg
     6361 tttctggaat ggcgtctgga ataacctctg agtagacgcg atccattgcc gactcccatc
     6421 cgtagtaaca agccgaatag ccgtcgcgct ggtagccgtg gtcttcaact ccacatccca
     6481 tgccctggtc atggtattca ggctgattgt cgggattaat gcagtattca atgaccgact
     6541 tcatccccgc attctccgcc gccagcgccg aaaacttctc gtgtgccaac ttaacagccg
     6601 catcagcctg cttaattgac tcaatcgctt tctggtggtc ttcgtacaga gccgaaatct
     6661 tggcctccgc ttcagcaaat ttacgcacca gatattcagc gtttgtttcg ttaaccttta
     6721 aatcacatgg gatgcattta cctttcagaa aaccatccat ctcaattagt gacatttgtt
     6781 tcatttcttc ccactccgcc acatagcatt cagatatttg ttgtcattaa cagaaccgaa
     6841 actctttctc ttaagtaatt cctctctcga tggcattggc tttacgcgtt ggcgaataat
     6901 catttctgcc ggaagaatgc cgggattgta tgcaagtcct ctcatggtaa attcctcagt
     6961 cattactgat agcgccatag cgtgagcggt aattacgcag gcgcgggtca atttcaggga
     7021 agtgggtata tgtggctttg cggaatggtc ggattgatgt ctggtaaatt cgctcgagtt
     7081 cttctttctc tgcaagccat atacagtggc gaaattcctt ttcctctttc gtttcctgcg
     7141 gtagcgacat tatccgatcg tagttttttc tgaatttatc cagcacctcc gatacggaat
     7201 tgccggaaca gcggcgtggg tcatccgcac catacagagg cgctggcata atggaatcct
     7261 tatgttgcta ctttagaagg gaattgaatc gtcgtattca ggatgatttt gatgattgct
     7321 actttgctgc tgttggctgt ttcctgaagt tgcaaatcca atctttgcat tcagtaattc
     7381 aagagtgatt gattgtccat tttgcccctg ataaacatca accctgatgt tttctccggt
     7441 aatttccaca atgccacctt caacaagaac actacggtag taatccgctt gcgctcccgg
     7501 cttggcaaat acaacggcgc tgtagtttgt ccattctttc ttttttgtct ggcgatcgta
     7561 atactgaacg ccagcacgga tgttgaatcc gatattttcc ccggcctgaa actctcttgc
     7621 gggcttgttt agtcttacag taatcgaatg tgccattaag cagccgctcc ttctaattcg
     7681 tctcgtctga tgttgtaaat gtcctgcgct ttgtgctgct ccggtgtgcc ttcgagcatc
     7741 ttccacgctt tggcgaacgc ctgtttaagc tcttccacgg tgtttttctg caatgctgcg
     7801 tcagtgaatg cttttagaac ctgttcaggt gtaggtgatg gttttgattg ctttgctgct
     7861 gcgttctgct gatgtttatg ctcgtcggta tctgcatctt tcgcatcatc aatgccgaac
     7921 aaaccattga ggcaatactt gcgtgcataa gagcttgtgg ctcccgtaac ttgtgcagaa
     7981 tccattcctt tcttgctttc ttcctctcgt gcaagagcgg ttgccgtatg actgttttcg
     8041 ccatcggtaa tagttgccgt ggctttcacg taataccgat caccaatcaa cacaacttca
     8101 tcgctgattg ataaaaacag accattcagt aacggcttaa cgccttcaag aatgtcttcg
     8161 cagcttctgt atttatattt accgaatgag ttgtactggt tctttggcgc gttcaagttc
     8221 tcctgaatag ctgccagtct tgcgtaaaat tctttgctca tatgtttgtt ctcagaatgg
     8281 gcatggccca aggaaataac gctgatttaa tacttcgact cgggacaaat taaggcatac
     8341 ccgcattcct tcgcggtcgc cattatggcg ataccagaga gctttctgcg tgtacatgcg
     8401 tctctgtaac ttgctctcct tcactgtggt tgcaagtgac atgaatatct ccttcgttac
     8461 cgattaattc tttcatctga cgaatgaatt cttcgtctga ccagttatct gtaaaactca
     8521 tttcctgcga taccacggaa tattgatagc tgatttcatc gctttatttg cttcaagcca
     8581 catttttgaa tcaccaataa atctggctat tactgctttg ttttgtgctg cacgaagcat
     8641 ctggtgattg atggctattt cattgcgcat aacgcctccg gttgtttctt tgctgcactg
     8701 attaattgtt taactcggcg tgataattca gattcgtgcg ggtagaaagc ggacatgacg
     8761 ccgctacccg cgagctgaaa gtgcatcatg gataactcct tctgtttgat tgcataacga
     8821 aaacgcctcg agtgaagcgt tattggtatg catataaaaa agccctcata ctggagggca
     8881 aagaagattt caaataatca gaacaagtcg gctcctgttt agttacgagc gacattgctc
     8941 cgtgtattca ctcgttggaa tgaatacaca gtgcttactc gtactaataa aatacccaat
     9001 tttctgtttc ttggttgtgc ccaaagttat attcaatatc tggtgttgat gtatcaatat
     9061 tcttcatccc atcaacaaga gttgatacaa cagccaaatc ctgtttgatt ctcattaaat
     9121 ggtatttctt ccggcgcaat aaactttcaa tggcaagttt cttcgttggg aatgcaaaag
     9181 atctttctgc attttttgct actttcttaa ttgcatatct atttctcttt tgtttccatt
     9241 cctgtaacca ctgatttggt gctggtttaa aattaacaat ccaatgcgca ggaaccaacc
     9301 atgcataatg ctctgtctga tgaaaagcta tatattgaag tgcgaatatt ttgattccat
     9361 cttcttcaac tgtcgcctgg aatctccaga aaacaggcat tccatcatgt tcagtttctg
     9421 attcaggaaa aggtacgctc catgattttg tcatatctca cctcaaataa gtggcttgct
     9481 gcctaatttc attttctggc gaccaacaca agtcacaccc atttcactgc gtggcttgct
     9541 gtaccatgtg cgctgattct tgcgctcaat acgttgcagg ttgctttcaa tctgttcgtg
     9601 gtattcagcc agcaccgtaa ggtctatcgg attcagtgcg ctttctactc gtgatttcgg
     9661 tttgcgattc agcgagagaa tagggcggtt aactggtttt gcgcttaccc caaccaacag
     9721 gggatttgct gctttccatt gagcctgttt ctctgcgcga cgttcgcggc ggcgtgtttg
     9781 tgcatccatc ttgattctcc tgtcagttag ctttggtggt gtggtaggtg ggagacccat
     9841 ttcgacctgc ttcggccgac ttcaattcgg caatagttcc gcaggcctcg ccgctttacg
     9901 tgcgacatat tcccatccat gaacccttca ccacacccca aagccttctg ctttgaatgc
     9961 tgcccttctt cagggcttaa tttttaagag cctcaccttc atggtggtca gtgcgtcctg
    10021 ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat tgtaaaaagg
    10081 ggcgtaaaaa caaactccat tgtttttaaa tgaaaaatag tttgtttttg gttatcgaga
    10141 ttgaggtggg gattactggt tgcaggttcc gactacatca ccaacaaatg atttggttga
    10201 tgtaagttgt tgcatacctg ggatattcat tactttggag taaagagctt ttttgtctgt
    10261 agtgattgat caagtttcaa cggttattcc tcctccagac tggtattctc ctaccatagt
    10321 gttcgatgac aaagcagtgt atttcatctc tggatagacg ccagaaactg attcataaac
    10381 tgatgattta tcgccattta ttgttacgtg gaaaacggaa tcttccgtgc tgtcttttgt
    10441 aaactcgtaa cgatcgccat tcattgaccc gtacccgtgc aggtttgtga caatccagca
    10501 ttcagaattg gcgctggtag ttaagagtat tgagagtagc gccgcaatcc tgatcatacg
    10561 aattttaccc tcgcttccac gacaacaccg ataatcttgc agttcccgtt gataggagtc
    10621 ataggccatg aagggttcag gcctttcagg tacttctgcc cgccatctat aaccagtttc
    10681 ttgaatgttg cttcgttcgc gtcagtcagt ttggctacaa caaggcttcc attcactggc
    10741 tcgcgtccag tatctactaa caccatatga ccttcaggga tgctttgacc tacaggtgag
    10801 gtcatggaat caccttcaac cttcagccag aatccatcgc ctaataagtt aacgtcactg
    10861 tcataccatt catcaatgtc cttgatatcg tagggttcac aagcttcaca ccacgaacca
    10921 gctctaacca tgctaatcaa tggatatttc cctttgggct caacatgccc aacaaatcta
    10981 acattcgaat cagaggtgcc attgagcagc cagtcaacac ttacgccaag agctgacgca
    11041 agttctggta aaaagcgtgg tcgcttagtt ttaccgtttt cgagctgctc tatagactgc
    11101 tgggtagtcc ccaccttttg agcaagttca gcctggttaa gtccaagctg aattcttttg
    11161 ctttttaccc tggaagaaat actcataagc cacctctgtt atttacctcc aatcttcaca
    11221 agaaaaactg tatttgacaa acaagataca ttgtatgaaa atacaagaaa gtttgttgat
    11281 ggaggcgata tgcaaactct ttctgaacgc ctcaagaaga ggcgaattgc gttaaaaatg
    11341 acgcaaaccg aactggcaac caaagccggt gttaaacagc aatcaattca actgattgaa
    11401 gctggagtaa ccaagcgacc gcgcttcttg tttgagattg ctatggcgct taactgtgat
    11461 ccggtttggt tacagtacgg aactaaacgc ggtaaagccg cttaagacat tcccgctctt
    11521 acacatccca gccctgaaaa agggcattac cagaaacaaa tctctatggt tttgcgtttc
    11581 tttgcgaagc caactctatc taatcattaa ggaaattatc tatgggtact attgcaacta
    11641 aaagcaagaa agcggctcgc atcgagtcag ccttgctgaa caaactggca ctgatggggc
    11701 agaagacatt cgctcgagca atgggggttc ctgaatatca ggtaagccga tggaagaatg
    11761 gtttcttctc gcaggtaagc atgatgcttg ctgttctgga atacggaatc gaagacgatg
    11821 aaatggctga attgactaag cggcttgccg attacctgac aaaagaaaaa gccccgaaga
    11881 acggcgaatt cttcgaggcc taatgtagaa agactggatc aatccacggg agtaattatg
    11941 acaaaacgtc gtaagaaata ccaggaaaaa gaagagattc gacaccctga ttcacctgag
    12001 ggattagtgg tagccgcagc aaataacagg gcgttcgcag agcgccttgt tggtgtttac
    12061 agactagcca aagcaggagt gaaacatggg cgtcgttaag ttagctgatt acaggcctca
    12121 actggaggtc gtggagcatc gcgtggcaga actcgaagat ggctacactc ggactgcaaa
    12181 cacactgtta gaagccgcca tgctttctgg acttactcta catcagttac tgattgttat
    12241 ggctgtgtgg cgcaagacat acggttacaa caaaaaaata gattggatcg gaaacgaaca
    12301 gttcgctgaa ctcactggca tggcaccaac caaatgctct accgccaaaa acgagcttat
    12361 cagaatggga gttctcattc aggtggggcg tcaagttggt atgaatacaa acatttccga
    12421 gtggaaaacg aaagttaacg gattcggtaa aacatttacc aattcggtaa aacaaacctt
    12481 caccaaatcg gtaaaaagca atttaccgaa tcagtcaaac acaaaagaca atatacaaaa
    12541 gacaataaat acaaataccc ccttaccccc taacgggggc ggcgatgggc aggttaaacc
    12601 tgaacgtcgc aaggcagaac gaatcgacta cgaatccttc ctgaacgcct acaacaccga
    12661 agtcggtgac agactgccac acgctgttgc ggtcaacgag aaacgcaaac gccgcctgaa
    12721 gaaaatcatc ccgcaactga aaacgccaaa cgtggacggt ttcagagcgt atgtcagggc
    12781 gtttgtgcat caggccaagc cgttttactt cggagacaac gacacgggct ggacggccga
    12841 ttttgattac ctgctgaggg aagactcgtt aacgggggta cgggaaggga agtttgcaga
    12901 cagggggatt gcatgaaaca ggatatcgaa gcgagcgtta tcggtggcct gctgattggt
    12961 ggattaacac caaccgccag cgacgttctg gcaacgctgg agccggaagc gttttcaatt
    13021 ccgctctacc ggaaagcctt cgaggttatc cgcaagcagg cgcgaaacag aaacctaatc
    13081 gacgcgctga tggttgccga ggcgtgcgga gaggagcatt tcacgtcaat cctgatgacc
    13141 agcaagaact gcccgagtgc cgcaaacctg aagggatatg ctggaatggt cgcggataac
    13201 tatcaccgcc gtctggtgct ggaaatcatg gatgaaatgc gtgaaccaat ccaaagcgga
    13261 accatcgacg catcgagtca ggcgatggat gaacttgtaa agcgtctttc agccatcaga
    13321 aagccccgtg acgaggtaaa acctgtacgg ttaggggaaa tcatcactga ctacactgac
    13381 acgcttgaca ggcgtctgag gaacggagaa gagtcagata ccctgaagac cggaatcgac
    13441 gaacttgacg ccatcaccgg agggatgaac gcagaagacc tggtgattat cgccgctcgt
    13501 cctggtatgg gtaaaaccga actggcgctg aagattgccg aaggcgttgc aagccgcgtt
    13561 attcctggtt ctgacgtccg gcgcggagtg ttgattttct cgatggaaat gagcgcattg
    13621 cagattgcag agcggagcat tgccaacgcc gggaggatgt cggttagcgt gctgcgaaat
    13681 cctgcatcga tggatgacga aggctgggcg cgtgttgcta acggcatgag tcagcttgca
    13741 gatttggatg tatgggtagt cgatgcctcg cggttatcgg tcgaagaaat acgctcaatc
    13801 gcagaacggc acaaacagga aaatccaaac ctgtcactca tcatggcgga ttatcttggc
    13861 ctgattgaga agccgaaagc agaccgcaac gacctcgcaa ttgctcacat ctccggaagc
    13921 ctgaaggcga tggcgaaaga cctgaaaacg ccagttatct ccctgagtca gctttcgcgc
    13981 gatgttgaga agcgaccaaa caaacgcccg acaaacgcag atttgcgtga ttcaggaagc
    14041 attgaacagg acgcagactc aatcatcatg ctctatcggg aagcggtata tgacgagaac
    14101 agtagcgccg cgccatttgc tgaaatcatc gtgacgaaaa accgttttgg ctcgcttggt
    14161 acggtttacc agcggttctg taacggacac tttgttgcat gtgaccagga tgaagccaga
    14221 cagatttgca cagcatcaaa tgcacctgct gcgcgtggca gacgatatgc acaaggggct
    14281 gacgtatgac catctacatc actgagataa taacaggcct gctggtaatc gcaggccttt
    14341 ttatttgggg gagagggaag tgtggctgac tggcaaattc caatcatcat tcttgccgga
    14401 gcttcgctgg ttgctggctt tatcctgctg aaaaagcata aagaccgtga tcaaaaagtt
    14461 gaagttctct atgggtatcc agcgaacagc acaacatggc tgaccattta ccactaccga
    14521 aaatcaggac gctgggtatt cgaatgggat gatctgttcg ctgaaaagcg accaaagtca
    14581 tggggagaca tcagcgaatg catgatgttt gaagaaagaa aatccggcgc aacccgagaa
    14641 gagtttaacg aagcgtgggc gcgattaagt gagagagggt atttgtgagc aagtacgaaa
    14701 aattagatca aaacattctt tcaatgctga gtgaaagacc aacacctgtt tttgatatct
    14761 ggcttaaatg gcggagcaat ggaatgtata tcgaaaccat cgatcgccgt atgcaatacc
    14821 tgagaaagaa agggcttgtt gcaaatgtgc gtgggaaggg ttgggtgaaa attaacctgt
    14881 cataacgggg attgatatgg acgatagctt agaaatattt gaagagtggt gcgctgatga
    14941 acttggtgtc tccgcttact tcattcgaca aatgcgaagc aagaatattt tgggcgtgat
    15001 tgagtataaa cgagttgaaa taaacaagag atacagggct tggatggccg cagttcgcgc
    15061 cgccggagtc aaagtgaagg agtgagtatg agcgcatacg aagaaatcat gttagccctg
    15121 cgattctttt tcggtgtgga agaagatgaa aacgtaaatg agattatcgg acaagaccat
    15181 gacccgatag ggactattgc agctgcactt gacgattaca ggagcgtaaa tggtgaggaa
    15241 actaacgttt gaactaagaa gccccatcca tcagcagaac gccattcaag ctatccagca
    15301 aatccttcca gacccaacca aaccaatcgt agtaaccatt caggaacgca accgcagctt
    15361 agaccagaat cgaaagcttt gggcttgcct tggtgacgtt tcgcgtcagg ttgaatggca
    15421 tggtcgctgg ctggatgcag aaagctggaa gtgtgtgttt accgcagcat taaagcagca
    15481 ggacgttgtt cctaaccttg ccgggaatgg atttgtggta ataggccggt caaccagcag
    15541 gatgcgtgta ggcgaatttg cggagctatt agagcttata caggcattcg gtacagaacg
    15601 tggcgttaaa tggtcagacg aagcgcgact ggctctggag tggaaagcga gatggggaga
    15661 taaaactgaa tgaacaaata ccgacttatt tacgcagatc cgccttggca atatcgcgac
    15721 aaagccaacg atggcaatcg cggtgctgga cataaatacg atgttatgaa tgttcaggac
    15781 atttgccgac tgccagtatg ggatttagcg gatccagaat cttgcttgtt agcgatgtgg
    15841 tgggtgccga cacagccagc cgaagcgcta aaggtaattg aggcgtgggg attcaggttg
    15901 atgactatga aaggctttac ttggcacaaa accaataagc acaaaggcaa cagtgcgatc
    15961 ggaatggggc atatgaccag ggcaaatagc gaggattgct tgtttgccgt tcgagggcgg
    16021 ttacctgaga gaatggacgc ttcaatatgc cagcacttta ccgcaccgag aatggagcac
    16081 agtgcaaaac caccgatcgt aagagacatg ttagctaagt tgcttggcga cgtgccgcgc
    16141 tgtgagttat ttagccgcga caaagtgacc gggtgggata tgtggggaaa tcagtgcgac
    16201 tccgattttg aacttgctcc cggcatggcg attaaaccat gcaaaatggt gatcgcatga
    16261 agcactgtta tcgatgtgga gagcgaaagg aagacgatcg ctttcgacca ggacagcctt
    16321 actggaatcg atggtgtctc cggtgtgaaa gaacaccaac aggggtgtta ccactaccgc
    16381 aggaaaagga ggacgtgtgg cgagacagcg acgaagtatc accgacataa tctgtgaaac
    16441 tgcaaatacc ttccaacgaa acgctccaga aataaacgca agccaatccc aaaagaatct
    16501 gacgtaaaaa ccttcaacta cacggctcac ctgtgggata tccggtggct aagacatcgt
    16561 gcgaggaaat gacaatgctt ttaattcaac ctggatttgg acttagcatc aaaaaaggcc
    16621 acatgtttgg cgagaaagag tcacaacgaa aaatgatgtc tatccggttg ccatttatca
    16681 gtatttattg gctaaacagg gaggcaacaa attattggta tacatgcgcc agagcagcat
    16741 ttaacgaccc tgactggttt gtggaaaacc accacgcagt tcgtcaggca aagagaaagg
    16801 ccaacatgac atacatgaag gcgtatcgaa aagcatggaa agaacaccgc gatcgatacc
    16861 agcaagatat ggaaaagctt gaatcagaaa acatggaatt aagacgaaag cttggtgaag
    16921 caaaacgaga cattgatgct tacaagcgac tttttaatgg tgaaagccat gcttagtcca
    16981 tcccaatctc ttcaatacca gaaagaaagc gtcgagcggg ctttaacgtg cgctaactgc
    17041 ggtcagaagc tgcatgtgct ggaagttcac gtgtgtgagc actgctgcgc agaactgatg
    17101 agcgatccga atagttcaat gtacgaggaa gaagacgatg gccaaaccag cgcgaagacg
    17161 atgtaaaaac gaagaatgtc gggaatggtt tcaccctgca ttcgctaatc agtggtggtg
    17221 ctctccagag tgtggaacca agatagcact cgaacgacga agtaaagaac gcgaaaaagc
    17281 ggaaaaagca gcagagaaga aacgacgacg agaggagcag aaacagaaag ataaacttaa
    17341 gattcgaaaa ctcgccttaa agccccgcag ttactggatt aaacaagccc aacaagccgt
    17401 aaacgccttc atcagagaaa gagaccgcga cttaccatgt atctcgtgcg gaacgctcac
    17461 gtctgctcag tgggatgccg gacattaccg gacaactgct gcggcacctc aactccgatt
    17521 tgatgaacgc aatattcaca agcaatgcgt ggtgtgcaac cagcacaaaa gcggaaatct
    17581 cgttccgtat cgcgtcgaac tgattaaccg catcgggcag gaagcagtag acgaaatcga
    17641 atcaaaccat aaccgccatc gctggactgt cgtagagtgc aaggcgatca aggcagagta
    17701 ccaacagaaa ctcaaagacc tgcgaaatag cagaagtgag gccgcatgac gttctcagta
    17761 aaaaccattc cagacatgct cgttgaagca tacggaaacc agacagaagt agcacgcaga
    17821 ctgaaatgta gtcgcggtac ggtcagaaaa tacgttgatg ataaagacgg gaaaatgcac
    17881 gccatcgtca acgacgttct catggttcat cgcggatgga gtgaaagaga tgcgctatta
    17941 cgaaaaaatt gatggcagca aataccgaaa tatttgggta gttggcgatc tgcacggatg
    18001 ctacacgaac ctgatgaaaa aactggagac gataggattc gacaccaaaa aagacctgct
    18061 tatctcggtt ggcgatttgg tcgatcgcgg tacagagaac gtcgaatgcc tggaattaat
    18121 cacattcccc tggttcagag ctgtacgtgg aaaccatgag caaatgatga ttgatggctt
    18181 atcagagcgt ggaaacgtca atcactggct acttaatggc ggcggctggt tctttaatat
    18241 cgattacgac aaagaaattc tggctaaagc tctttcccat aaagcagatg aacttccgtt
    18301 aatcatcgaa ctggtgagca aagataaaaa atatgtcatc tgccacgccg attatccttg
    18361 tgacgaatac gagtttggaa agccagttga tcatcagcag gtaatctgga accgcgaacg
    18421 aatcagcaac tcacaagacg ggatcgtgaa agaaatcaaa ggcgcggaca cgttcatctt
    18481 tggtcatacg ccagcagtga aaccactcaa atttgccaac cagatgtata tcgataccgg
    18541 cgcagtgttc tgcggaaacc tcacattgat tcaggtacag ggagaaggcg catgagactc
    18601 gaaagcgtag ctaaatttca ttcgccaaaa agcccgatga tgagtgactc accacgggcc
    18661 acggcttctg actctctttc cggtactgat gtgatggctg ctatggggat ggcgcaatca
    18721 caagccggat tcggaatggc tgcattctgc ggtaagcatg aactcagcca gaacgacaaa
    18781 caaaaggcta tcaactatct gatgcaattt gcacacaagg tatcggggaa ataccgtggt
    18841 gtggcaaagc tcgaaggaaa tactaaggca aaggtactgc aagtgctcgc aacattcgct
    18901 tatgcggatt attgccgtag tgccgcgacg cctggcgcaa gatgcagaga ttgccacggt
    18961 acaggtcgtg cggttgatat agccaaaaca gagcagtggg ggagagttgt cgagaaagag
    19021 tgcggaagat gcaaaggcgt cggctattca aggatgccag caagcgcagc atatcgcgct
    19081 gtaacgatgc taatcccaaa ccttacccaa cccacctggt cacgcactgt taagccgctg
    19141 tatgacgctc tggtggtgca atgccacaag gaagagtcaa tcgcagacaa cattttgaat
    19201 gcggtcacac gttagcagca tgattgccac ggatggcaac atattaacgg catgatattg
    19261 acttattgaa taaaattggg taaatttgac tcaacgatgg ataaatgcac tcgttaaata
    19321 aagccctgag ttaatagctc ggggcttttt gcgttttaag cacggccttt ctgaaagcac
    19381 atcaagccaa ataccagaca gacaaaaata atcaccttat ccgcttggct acggtgcggt
    19441 gtgctttgca taaaagaaaa ccagcgcaat ggctggcttc gtgaaagcgg gtggcaagag
    19501 gctgcgctaa caacctcatg ccgttttgcc cgtgcatatc ggtcacgaac aaatctgatt
    19561 actaaacaca gtagcctgga tttgttctat cagtaatcga ccttattcct aattaaatag
    19621 agcaaatccc cttattgggg gtaagacatg aagatgccag aaaaacatga cctgttagcc
    19681 gccattctcg cggcaaagga acaaggcatc ggggcaatcc ttgcgtttgc aatggcgtac
    19741 cttcgcggca gatataatgg cggtgcgttt acaaaaacag taatcgacgc aacgatgtgc
    19801 gccattatcg cctggttcat tcgtgacctt ctcgacttcg ccggactaag tagcaatctc
    19861 gcttatataa cgagcgtgtt catcggctac atcggtactg actcgattgg ttcgcttatc
    19921 aaacgcttcg ctgctaaaaa agccggagta gaagatggtg gaaatcaata atcaacgtaa
    19981 ggcgttcctc gatatgctgg cgtggtcaga gggaacagat aacggacgac agaaaaccag
    20041 aaatcatggt tatgacgtca ttgtaggcgg agagctattt actgattact ccgatcaccc
    20101 tcgcaaactt gtcacgctaa acccaaaact caaatcaaca gcagccgggc gctatcagct
    20161 tctttcccgt tggtgggatg cctaccgtaa gcagcttggc ctgaaagact tctctccgaa
    20221 aagccaggac gctgtggcat tgcaacagat taaagagcgt ggcgctttgc cgatgattga
    20281 tcgcggtgat atccgtcagg caatcgaccg ttgcagcaat atctgggctt cgttgcccgg
    20341 ggctggttat ggccagttcg agcataaggc tgacagcctg attgcaaaat tcaaagaagc
    20401 aggcggaacg gtcagagaga ttgaggtatg agcagagtaa ccgcgattat ctccgctctg
    20461 gttatctgca tcatcgtctg cctgtcatgg gctgttaatc attaccgtga taacgccatc
    20521 gcctacaaag agcagcgcga caaagccgca tccactatcg ctgatatgca gaagcgtcaa
    20581 cgtgacgtag cagaactcga cgccagatat acaaaggagc ttgctgatgc taacgcgact
    20641 atcgaaagcc tccgtgctga tgtttctgct ggtcgtaagc gcctgcaagt cgccgccacc
    20701 tgtgcaaagt caacgaccgg agccagcagc atgggcgatg gagaaagccc aagacttaca
    20761 gcagatgctg aactcaatta ttaccgtctc cgaagcggaa tcgacaggat aaccgcgcag
    20821 gttaactacc tgcaggaata catcaggact cagtgcctga aataattttt ttgcaaatca
    20881 caaagtccat ttaatgagcc tcgcgatgcg gggctttttt acatctgaat ttcacagcgc
    20941 atctcacgcg catattacat cacccgagcc tttcagaaag ttgagcctga gaactgccgt
    21001 atatggtggc gaccatctcg ggacggcttt tctgtgagac aggctcactt tctaaaaggt
    21061 aaagacgcaa tgaactaccc aaccgttgtt aacgatatag atttcagaga cctaattttt
    21121 gtagcaaaca acgatccggt tacagattct tttatggtgg caaaagcatt tggaaagctg
    21181 ccgaagaacg tagttcgtga cattgagcga accatagaag cttgccctcc tgagtttgat
    21241 acaaagctca actttgagct ttgctataaa aacaatgagt tacagaatgg taagccgcaa
    21301 aaattctacc gtctccgcaa ggatgggttg atgcttttgg ttatgtccta caccaaaaaa
    21361 gaagcaatgc gtatcaaaat tgcttacatc aacgcattta actggatgta cgccatgctt
    21421 caggttggtc atcgtcaatt tgaagaagag agaaatgccg taatgctgga gtacatgaaa
    21481 gagaaggatg ttgccagcat gtcaggtcgc ctgctaaatc gctggggcaa aattaagaag
    21541 ccacagctga tggctagaat tgaacgcctt gaacagcacg ggcaaaccgt aatccccgga
    21601 ctcatcaatt aacggcagca ccgcgaaaca acccaagcca gtaagtgggg aaataacact
    21661 ggcagccact gaaagatgaa cctccagcct tatggcaaaa aagattcttt gtggtggcgg
    21721 actgatggaa agacatcgga tagaatcaag cagtggctag ggtagctccc gaaaagcgga
    21781 ctcgtcaccg cctgccactg aatctatggc gaacaactag acgaggttgt gatggatgat
    21841 aacggaaacc aatgggtcaa tgttgaacac cggctaccag aaagcaaaga agggatgtgg
    21901 tctaaagagg ttatagctct tactgatact ggtgacgtgt tcaaactatc atgtatgggc
    21961 tcttactggc agagaaccaa ggcattcatc gattcaggag caagcaaggt tacacactgg
    22021 atgccgctta attacccaga tgattaaaac ggatgaaaaa tgaaaggtcg ctcaggcggc
    22081 cttttttatt gccatcacaa aagccattcc ttactgagtg gctttgataa tggcttatac
    22141 cctacacggg ataacttaac tgatatccct tttaacggat aaatggagcc aacaatggca
    22201 gagattattc ccatgactga agaacagaaa ttccagttag agatttacaa actggtcatg
    22261 aaccagaacg cagccgcaga agaagcattt caattcattg gcactgacga actgaagctt
    22321 gagctattca aaattcactt ccagtcaggt ggcgctaatt cagatatcac gatccgcaca
    22381 tttgaagcgg tgcgtaaatc gaaggaagcg ttagacctgt tcaccaccgg agcatgatgc
    22441 tcaacctgaa ataacgatta agtgagatga atatggcagc accaaagggc aaccgattct
    22501 gggaggcccg cagtagtcat gggcgaaacc ctaaattcga atcgcctgag gcgctgtggg
    22561 ctgcttgttg tgaatacttc gagtgggctg atgataaccc gctatgggag ggtaaggtat
    22621 tttcatatca gggagaaata attaaggcta atgtccctaa gatgcgagcc atgactattt
    22681 caggattgtg taccttcctt gatatcacca ggcaaacatg gggaaccttc cggtcaatgg
    22741 aaggtttttc tgacgtcaca tcacgagcgg aagacatcat ctacgaccag aaattctctg
    22801 gcgcagccgc tgaccttctc aacgctaaca tcatcgcccg tgatttgggc ctcaaagagc
    22861 agtcgcaagt tgaagacgtg acacctgata agggagatcg cgataagcgg cgctctcgta
    22921 tcaaggagct attcaaccgt ggaactggac gcgattcttg ataacttgag cgacgaagag
    22981 caaatcgaat tgctcgagct actcgaagaa gaagagaact accgtaacac acacctgcta
    23041 tatgaattta cgccatacag caaacagcgt gagttcatcg acgccgggca tgactatcca
    23101 gagcgctgtt ttatggctgg taaccagctt ggtaagtcat ttactggtgc tgctgaagtc
    23161 gcgtttcacc ttaccgggcg ttatccgggc acaaaaggct atcctgctga tggtaaatat
    23221 ggcggtgagt ggaaaggtaa gcgtttttat gagcctgttg tcttttggat tggtggcgag
    23281 acaaacgaga ctgtaaccaa aacgactcaa cgcatcctgt gtggtcgtat cgaagagaat
    23341 ggtgagccag gctacggttc catacctaaa gaagacatca ttagctggaa gaagtctcct
    23401 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
    23461 gatggcattt caatctgcta cttcaagcca tactcgcaag gtcgtgctcg ctggcagggt
    23521 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggtgaaggc
    23581 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
    23641 atgtctgacg ttgttaccaa gttcctgaag aatccaagca agtcgcagaa agtggtcaac
    23701 atgaccatct acgacgctga gcactacaca gacgaacaga aagagcaaat catcgcatct
    23761 tatcctgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
    23821 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
    23881 tacgtaattg gcgggatgga tttcggatgg gatcacccgc aggcgcaggt tcagctttgg
    23941 tgggacaagg acgcagacac aatatacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
    24001 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac tgcatggcct
    24061 catgacggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagat
    24121 gctggtttta tgatgttgca ggagcatgcg acatggcctg atggcggtaa tgctgtggag
    24181 cctggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaacacc
    24241 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
    24301 aagcttaacg acgacgttct ctcagccgtt cgctatgcat acatgatgcg ccgcttcgcc
    24361 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
    24421 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
    24481 gacgcggact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
    24541 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
    24601 caattcga
//
LOCUS       DAJTYL010000058        24090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYL010000058 DAJTYL010000000
VERSION     DAJTYL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.058"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="OO572_002259"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="OO572_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6661244.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="OO572_002260"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="OO572_002260"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6661245.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="OO572_002261"
     CDS             1532..2479
                     /locus_tag="OO572_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6661246.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="OO572_002262"
     CDS             2549..3355
                     /locus_tag="OO572_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6661247.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="OO572_002263"
     CDS             3365..3772
                     /locus_tag="OO572_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6661248.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3772..4266
                     /locus_tag="OO572_002264"
     CDS             3772..4266
                     /locus_tag="OO572_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6661249.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="OO572_002265"
     CDS             4332..5129
                     /locus_tag="OO572_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6661250.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="OO572_002266"
     CDS             5140..5928
                     /locus_tag="OO572_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6661251.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="OO572_002267"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="OO572_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6661252.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="OO572_002268"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="OO572_002268"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6661253.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="OO572_002269"
     CDS             complement(8371..10002)
                     /locus_tag="OO572_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6661254.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="OO572_002270"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="OO572_002270"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6661255.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="OO572_002271"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="OO572_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6661256.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15246..17430
                     /gene="espL4"
                     /locus_tag="OO572_002272"
                     /pseudo
     CDS             15246..17430
                     /gene="espL4"
                     /locus_tag="OO572_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17399..19136)
                     /gene="aceK"
                     /locus_tag="OO572_002273"
                     /pseudo
     CDS             complement(17399..19136)
                     /gene="aceK"
                     /locus_tag="OO572_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19320..20624)
                     /gene="aceA"
                     /locus_tag="OO572_002274"
     CDS             complement(19320..20624)
                     /gene="aceA"
                     /locus_tag="OO572_002274"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6661257.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20654..22255)
                     /gene="aceB"
                     /locus_tag="OO572_002275"
                     /pseudo
     CDS             complement(20654..22255)
                     /gene="aceB"
                     /locus_tag="OO572_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22524..23453)
                     /gene="metA"
                     /locus_tag="OO572_002276"
     CDS             complement(22524..23453)
                     /gene="metA"
                     /locus_tag="OO572_002276"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6661258.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23610..24053
                     /locus_tag="OO572_002277"
     CDS             23610..24053
                     /locus_tag="OO572_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6661259.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5944 a         6146 c         6109 g         5891 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatcgc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggaaaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtggaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc agggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggtgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tggtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cacacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggtggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag attctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatgg tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgaggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gcttaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cacttaatta ttaacaaaat cgcaagatct cattccaccg
    15121 aaacttttac ggctgcatta tgagattgag cgatttatga tattttttta gttgtaatca
    15181 tattaaattt tttcactgtt ctgatagtta aaattcaaga catcaataaa caatgagata
    15241 tttaaatgat tactcgtatt cctcgtagtt ctttctctgc aaatattaat aatacagccc
    15301 agacaaatga acaccaaacc ctgagtgaat tgttttataa agaactcgag gataaatttt
    15361 ctggcaagga gctggcgacg cctctattaa aaagcttctc agagaactgt agacaaaatg
    15421 ttcgtcatat ctttagcaac aaggattttg tcattaaatt ttccacgtcc gtcttacaag
    15481 ctgataagaa agaaattacg atgattaata aaaacgaaaa cacgacactc actcaaacca
    15541 ttgctacaat atttgaagaa tacctaatgg aaattttacc tcaacgctca gacactcttg
    15601 ataaacaaga attaaaccaa aaatcagata gaaaagaaaa agaattccca agaattaaac
    15661 ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt atagtatacc gacacattgc
    15721 tgcacaatat attaatgata tttatcagaa tgttgattac aaaccccatc aagatgatta
    15781 ctcttcagct gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc agactttggc
    15841 gttgatttcc agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt
    15901 tatgaaagca tattttgaca agatggaatc aaatggcatc agtgttatgg cagccatatt
    15961 actggtggat aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg
    16021 tacccattac gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaattat
    16081 gagcgaaagc aaagaggata ttaaacacta ttctctgatg gattttatga atgtagatta
    16141 tagcctcctg aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc
    16201 acttccgaaa gaacagctat tgatgttaaa aggaactgtg gatgaaataa cccctccatt
    16261 atcaccagca acgatgaatt tgctaatggc aattggtcag aatcaccaac ttacgcaact
    16321 gatgattcag ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa
    16381 tagtattaac ctccccggtt tatatttggc tataaattat ggcaatgcgg atatcgttga
    16441 gactattttc aattcattgt cagaaacagg atatgaaggg ttactctcga aaaaaaatct
    16501 catgcatatt ctggaggcaa aagataaaaa tggtttttct ggattatttt tagcgatatc
    16561 acgtaaggat aaaaatgttg taacctcgat tctgaacgtc ttacctaaac tggccgcaac
    16621 acatcattta gataatgaac aggtgtataa attcctgagt gccaaaaata gaacgtccag
    16681 ccatgtttta taccatgtta tggcgaatgg tgatgccgac atgctgaaaa tttttttggt
    16741 cgcgttacct ttgttaattc gcacatgtca tttgactaaa gaacaggtac tcgatctcct
    16801 gaaggcaaag gatttttatg gttgcccaag actatacctg gcgatgcaaa atggacatag
    16861 cgatatcgtg aaagttattc tcgaagcatt gccctgtctg gcccaggaaa ttaacatttc
    16921 agcttccgat attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt
    16981 tatggccatg cagcgcggac atatgaacgt tattaatact atttttaacg cattacccac
    17041 tctttttaat acgtttaaat tcgataaaaa aatatgaagc ccctcctcct ggcaaataat
    17101 tctaatgaat atcccggttt gttttcagcg atacagcata aacaacaaaa cgttgtagag
    17161 acggtttatc ttgctttatc tgaccatgca cgcctgtttg gatttaccgc tgaagatatt
    17221 atggattttt ggcaacacaa agcgccacaa aaatactctg cctttgagtt ggcttttgaa
    17281 tttggtcacc gggttattgc tgaattaatc cttaatacat taaataagat ggctgaaagc
    17341 tttggcttta cggataaccc tcgatacatt gcggagaaaa attatatgga agctttactc
    17401 aaaaaagcat ctccccatac cgtacgctaa atctttgcct gcgccgatac gcataaacat
    17461 cttccacatg cccttcacgt atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca
    17521 ggtcggcgtg catctcttca aacagcggac caatacgcgg gtcggcgcat agccagtggc
    17581 gaaactcttc cgggaaaaca tcgcccggcg agacgctgta ccacggttcg ctggcaagtt
    17641 cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa
    17701 tttcatcgta atcataaaaa accacacgcc cgtgacgggt gacaccgaag tttttaaaca
    17761 gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg aatagcgtta ccgtattctt
    17821 caatggcgtc gcgcaactgc tgaccttcca cttgttccag ccagatattg agcggcacca
    17881 tccgccgctc gattatagag atggcgaatt acaatctgct caccaagatc ggtgattttt
    17941 tccgctgctt cctggagcag taattccatt aataccgggg aaatatgccg cttctccagc
    18001 acaaagtttt caaactcctg ggtgtccgcc attcggccca cacggtcgtg ctctttcacc
    18061 agttgatagc aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct
    18121 ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa acaccaacat caccattcca
    18181 cgaatacccg gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg
    18241 cggtagcttt cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg
    18301 gttttacctg gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa
    18361 accataaaat aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg
    18421 caggtatcaa taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac
    18481 gtgccggaag gcgtgatcag tttgcccacc agccaggcgg ctttattgcg gtaaaacagt
    18541 tcgttcgcca cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga
    18601 tggcgaatga tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg
    18661 tcgctgataa cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc
    18721 agcgggcggg gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc
    18781 tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc
    18841 gcaatctcga agcgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt
    18901 aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga
    18961 ccaacgtgat gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag
    19021 tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga
    19081 gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag
    19141 cgccctcatc aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt
    19201 tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga
    19261 ttgccggatg cggcgtaaat gccttatccg gcctacagtc agcaacgttt gtaggttgct
    19321 tagaactgcg attcttcagt ggagccagtc agcgcggtga ctgaagacgt gccgccctga
    19381 ataatagtcg tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag
    19441 gtatagccat ctttcgcggc ggcaaattcc ggctgctgca ctttctcaac atagtgcttc
    19501 ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca tgttaaacca catgctgtgg
    19561 atacctgcta gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag
    19621 ctggcaatag ttttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa
    19681 gccagcagtt tgcccggata tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc
    19741 agatccggcg tggaggtttc acaccagacc aggtcagcat atggcgcata cgccaggcca
    19801 cggctgatcg cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg
    19861 ccggtaataa attcgctgtc atacgggtcg caatctgagg tgatcagatc cgccgcatca
    19921 gcatcggtac gtgcaaccag cagggttgga acgcccgtca cgtcagctgc cagacgcgcc
    19981 gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa ctttgccgcc catgtgaccg
    20041 catttcttca ctgacgccag ctgatcttcg aagtgaactg ccgcagcacc ggcttcaatc
    20101 atcgctttca tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcatcggca
    20161 acgatcggca ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cacggaccat
    20221 tggatctgat cggcacgacg gaaggtgttg ttgatccgct ccaccacagc tggcaccgag
    20281 tttgccggat agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct
    20341 acctgccatc ccgacagata gactgcttca atacccgctt tcgcctgttg cagcgcctga
    20401 ccgccagtca gtgcaccgag gttgttgatg tagccttttt tcgactcacc gtgcagcaga
    20461 cgccacattt tcgctgcgcc cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt
    20521 aatttcacca catcttccgc actgtatggg cgagtaatgc cttcccaacg cggttgagtc
    20581 cactctttct gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata
    20641 gttatgtggt ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact
    20701 catcggaagt ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgg ccctgggaga
    20761 aacgttcttc gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga
    20821 acaaggcttt ggtcaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc
    20881 agatcgaggt acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca
    20941 cgcagccgtt gccggagatc cacgcttcga tgtactgcac agccacgcga atgttagcgc
    21001 gcataccttc ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg
    21061 gcgcgtcttg ttcgcgcaat acttcaagct gatttttacg ggagccgaga atgtcgttga
    21121 ataccgccat tgccgtgtcc gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt
    21181 tattggcttc cagcgcttta tccgctttta ctttgttgag cacctggtta ttacgctctt
    21241 catctttgct cggaataaac gcggccatgc cgcccatcgc aaaagcaccg cgtttatggc
    21301 aggttttaat cagcaggcgt gagtaagcat tcaggaatgg tttatccatc gtcacggcct
    21361 gtctgtctgg caggacacga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt
    21421 aatcccagcg accgcagttc agaccaacaa tatggtcgcg cagcgcgtga aggatttcgt
    21481 ccatctggaa cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg
    21541 gcagattaaa gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgct
    21601 aggactgggt tttcggcaga tagaaatagg gaccgctgcc ctttgccaac agtgcctgat
    21661 agttgtggaa gaaatagagc gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc
    21721 aggtgacatg tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat
    21781 tgggcttgag ctgataaatt ttgcctgctt cattggtata gctgatggta ccgttaaccg
    21841 catcgcgcag gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt
    21901 cgaaatcggc cataaagact ttcacattgg cgttcagtgc gttgatcacc atcttgcgct
    21961 ctaccgggcc agttatctct acgcggcggt cttctaagtc cgcaggaatc ccgcgaattt
    22021 tccaatcagc atcgcgaatg gaagccgttt ccgaaataaa atcaggcaac gttccgttat
    22081 caatatcttg ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat
    22141 gcgtcaccag ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct
    22201 cgccatacgg ccttgtgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc
    22261 agctcctcgt catggatccg aaaacttccc ctctgtgaac gaaggaacgt tgtgcgcttt
    22321 tcgttcagca caactaagac tactcattta aaatgcaaaa tcaaaaacaa tttccatttt
    22381 aattaaaaat acttgataac ttattgataa caatatgaat aaaatttatt cacttgctga
    22441 ggtgcgtttc ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct
    22501 taacgatcga ctatcacaga agattaatcc agcgttggat tcatgtgccg tagatcgtat
    22561 ggcgtgatct gatagacgta atagttgagc cagttggtaa acagcaaatt accgtgacta
    22621 cgccagctcg ctcgcggttt gttttgcgga tcgttgtgcg ggaaatagtt atacggtaca
    22681 tccgggtcta gtccggcttc cacatcgcgt aaatattcct gcgccagcgt ttgcgcatca
    22741 tattcgggat ggcccgtcac aaaggcaatg cgcttatctt tgctggcaaa cagatatgca
    22801 tccccttctt ccgtctctgc cagaatttcc agatcggtgt aatcacgaat caacgctgcc
    22861 ggaaagtcag catagcgcga atgcggtgcc aggaatgaat catcaaagcc acgcgtcaga
    22921 agcgcatgag gatggagaat atgatgctcg taaacgccag agagtttgtc ggtgcgagtt
    22981 tgcttaggaa tgccgtagag gatattgagc gcggcctgta ccgcccagca gacaaacagc
    23041 gtcgaggtga cgtgatcttt cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg
    23101 acatcattaa actccaccag gcccagcggc gcaccggtta caatcagacc gtcaaagttc
    23161 tgttcctgaa tatcttcaaa gttacagtag aagttgttca gatgctctgc gggcgtgttg
    23221 cgcgattcac gggaatcgat gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa
    23281 agcaggcgca gaaactgatt ttcagtttca atcttcttcg gcatcaggtt aaggatcaga
    23341 accttaagtg gacgaatttc ctgaccagac gcacgagaag ttgtcatcac aaagacgttt
    23401 tcttcacgca agaaattgac ggcgggtagc tcgtccggca cacgaatcgg cataacctga
    23461 ttacctcact acatacgctt atacgtttag acatccagat agctgaagat aaccagaaaa
    23521 tttgccaatg tcgagcctgc atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta
    23581 gaatataaga atgacgaaaa ggagagaaaa tggttattag tattcgccgc tcacggcatg
    23641 aggaagggga ggaactcgtt gcgatttggt gtcgatctgt cgatgccacc cacgattttt
    23701 tatcagcaga gtatcgggcc gagctggaag agctggtgcg ttcctttctg ccggaagcac
    23761 cgttgtgggt cgcggttaat gaacgggagc agccggttgg gtttatgttg ttaagtgggc
    23821 agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc
    23881 tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat gagcaaaatg
    23941 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
    24001 acgatttggg gaaaccgtat ccgttgctga atctggcgta tgtgggggaa taagccgcct
    24061 ctgccgtgcc tgcccaagct ggagaagacg
//
LOCUS       DAJTYL010000059        23638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYL010000059 DAJTYL010000000
VERSION     DAJTYL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.059"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            367..2295
                     /gene="thrS"
                     /locus_tag="OO572_002278"
     CDS             367..2295
                     /gene="thrS"
                     /locus_tag="OO572_002278"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6661260.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2299..2841
                     /gene="infC"
                     /locus_tag="OO572_002279"
     CDS             2299..2841
                     /gene="infC"
                     /locus_tag="OO572_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6661261.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2938..3135
                     /gene="rpmI"
                     /locus_tag="OO572_002280"
     CDS             2938..3135
                     /gene="rpmI"
                     /locus_tag="OO572_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6661262.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3188..3544
                     /gene="rplT"
                     /locus_tag="OO572_002281"
     CDS             3188..3544
                     /gene="rplT"
                     /locus_tag="OO572_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6661263.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3661..3785
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3667..3711
                     /gene="pheM"
                     /locus_tag="OO572_002282"
     CDS             3667..3711
                     /gene="pheM"
                     /locus_tag="OO572_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6661264.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3994..4977
                     /gene="pheS"
                     /locus_tag="OO572_002283"
     CDS             3994..4977
                     /gene="pheS"
                     /locus_tag="OO572_002283"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6661265.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4992..7379
                     /gene="pheT"
                     /locus_tag="OO572_002284"
     CDS             4992..7379
                     /gene="pheT"
                     /locus_tag="OO572_002284"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6661266.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7384..7683
                     /gene="ihfA"
                     /locus_tag="OO572_002285"
     CDS             7384..7683
                     /gene="ihfA"
                     /locus_tag="OO572_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6661267.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7784..8764
                     /gene="btuC"
                     /locus_tag="OO572_002286"
     CDS             7784..8764
                     /gene="btuC"
                     /locus_tag="OO572_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6661268.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8827..9378
                     /gene="btuE"
                     /locus_tag="OO572_002287"
     CDS             8827..9378
                     /gene="btuE"
                     /locus_tag="OO572_002287"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6661269.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9378..10127
                     /gene="btuD"
                     /locus_tag="OO572_002288"
     CDS             9378..10127
                     /gene="btuD"
                     /locus_tag="OO572_002288"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6661270.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10205..10669
                     /locus_tag="OO572_002289"
     CDS             10205..10669
                     /locus_tag="OO572_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6661271.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10916..11629
                     /gene="ydiV"
                     /locus_tag="OO572_002290"
     CDS             10916..11629
                     /gene="ydiV"
                     /locus_tag="OO572_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6661272.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11693..13129
                     /locus_tag="OO572_002291"
     CDS             11693..13129
                     /locus_tag="OO572_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6661273.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13133..13324)
                     /gene="hemP"
                     /locus_tag="OO572_002292"
     CDS             complement(13133..13324)
                     /gene="hemP"
                     /locus_tag="OO572_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6661274.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13456..14502)
                     /gene="aroH"
                     /locus_tag="OO572_002293"
     CDS             complement(13456..14502)
                     /gene="aroH"
                     /locus_tag="OO572_002293"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6661275.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14659..15492)
                     /gene="ppsR"
                     /locus_tag="OO572_002294"
     CDS             complement(14659..15492)
                     /gene="ppsR"
                     /locus_tag="OO572_002294"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6661276.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15824..18202
                     /gene="ppsA"
                     /locus_tag="OO572_002295"
     CDS             15824..18202
                     /gene="ppsA"
                     /locus_tag="OO572_002295"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6661277.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18259..19905)
                     /gene="fadK"
                     /locus_tag="OO572_002296"
     CDS             complement(18259..19905)
                     /gene="fadK"
                     /locus_tag="OO572_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6661278.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(19962..20255)
                     /locus_tag="OO572_002297"
     CDS             complement(19962..20255)
                     /locus_tag="OO572_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6661279.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20252..21541)
                     /locus_tag="OO572_002298"
     CDS             complement(20252..21541)
                     /locus_tag="OO572_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6661280.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21597..22535)
                     /locus_tag="OO572_002299"
     CDS             complement(21597..22535)
                     /locus_tag="OO572_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6661281.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22555..23319)
                     /locus_tag="OO572_002300"
     CDS             complement(22555..23319)
                     /locus_tag="OO572_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6661282.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5815 a         5780 c         6242 g         5801 t
ORIGIN      
        1 taccgccaaa tcacgcaacg aactttgctg gattcagttt atgctgctgt aaatccgctc
       61 gcagtaaacc tttcagacgc accgtgatgt tatcagtggt tctttaagcg ttttgctggt
      121 gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt acaatctccc
      181 tttttttagt gttaacatcg ttcaaccggg tggaaggtga gaggttaatg tctttcattt
      241 gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc aatgaactgt
      301 taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg caaataagga
      361 tataaaatgc ctgttataac tcttcctgat ggcagccaac gccattacga ccacgctgta
      421 agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg tatcgcaggg
      481 cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc acaactgtcg
      541 atcattaccg ccaaagacga agaaggtctg gagatcattc gtcactcctg tgcgcacctg
      601 ttagggcatg cgattaaaca actttggccg cataccaaaa tggcaatcgg cccggttatt
      661 gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga agatgtcgaa
      721 gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat taagaagaaa
      781 gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta caaagtttcc
      841 attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca tgaagaatat
      901 gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt tctgccatca tttcaaacta
      961 atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt gcaacgtatt
     1021 tacggtacgg cgtgggcaga caaaaaagca cttaacgctt acctgcagcg cctggaagaa
     1081 gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca tatgcaggaa
     1141 gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg tgaactggaa
     1201 gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg tccgttcatg
     1261 atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga tgcaatgttc
     1321 accacatctt ctgagaaccg tgaatactgc attaagccga tgaactgccc gggtcacgta
     1381 caaattttca accaggggct gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt
     1441 ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtgga
     1501 tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga tgaagttaac
     1561 ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa gatcgtcgtc
     1621 aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg aaatgtggga tcgtgctgag
     1681 gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca actgggtgaa
     1741 ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga tcgtgcatgg
     1801 cagtgcggta cagtacagct ggacttctcc ttgccgtctc gtctgagcgc ctcctatgtg
     1861 ggcgaagaca acgagcgtaa ggtaccggta atgattcacc gcgcaattct ggggtcgatg
     1921 gagcgtttca tcggtatcct gaccgaagaa ttcgctggtt tcttcccgac ctggcttgcg
     1981 ccagttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt taacgaattg
     2041 acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa tgagaagatt
     2101 ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt ctgtggtgat
     2161 aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga cctgggaagc
     2221 atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg cagtcttaaa
     2281 caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt
     2341 atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt
     2401 ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc
     2461 gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc
     2521 tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag
     2581 gaaattaaat tccgtcctag tacagatgaa ggcgactatc aggtaaaact ccgcagcctg
     2641 attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag
     2701 atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa
     2761 ctggcagtag tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc
     2821 gctcctaaga agaaacagta aggccttcaa gtagcactat ctgtggagcc tttgggcttc
     2881 acaggcattg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt tattaaaatg
     2941 ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt
     3001 tttaagcaca agcacgctaa cctgcgtcac attctgacta aaaaagcgac caaacgtaaa
     3061 cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt aatcgcgtgc
     3121 ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata gatacaggag
     3181 agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt
     3241 gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc
     3301 tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc gtcagttccg
     3361 tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt cttacagcaa
     3421 attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc tggctgatat
     3481 cgcagtattc gacaaagtag cgttcaccac tctggttgaa aaagcgaaag cagctctggc
     3541 ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc atcaaaacat
     3601 tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca aaaggtaacg
     3661 caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg aatccaggag
     3721 gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt
     3781 tttgtatgcg cgttttgaaa aataaaaagc cagtctatca ggcgtaattg tcgcagccag
     3841 tctggacacg gccagtgcgg agaaaccgga gcgtacttaa gtacgtgagg atttcgagca
     3901 ctgcccggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta agtccaacga
     3961 accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga actggttgcc
     4021 agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagataa tgtgcgcgtc
     4081 gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg tgagctgccg
     4141 ccagaagagc gtccggcagc tggtgcggtt atcaacgaag cgaaagagca ggttcagcag
     4201 gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg tctggcggcg
     4261 gaaacgattg atgtctctct gccaggtcgt cgcattgaaa acggcggtct gcatccggtt
     4321 acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac cgtggcaacc
     4381 gggccggaaa tcgaagacga ttatcataac ttcgatgctc tgaacattcc tggtcaccac
     4441 ccggcgcgcg ctgaccacga cactttctgg tttgacgcta cccgcctgct gcgtacccag
     4501 acctctggcg tacagatccg caccatgaaa gcccagcagc caccgattcg tatcatcgcg
     4561 cctggccgtg tttatcgtaa cgactacgac cagactcaca cgccgatgtt ccatcagatg
     4621 gaaggtctga ttgttgatac caacatcagc tttaccaacc tgaaaggcac gctgcacgac
     4681 ttcctgcgta acttctttga ggaagatttg cagattcgct tccgtccttc ctacttcccg
     4741 tttaccgagc cttctgcaga agttgacgtc atgggtaaaa acggtaaatg gctggaagtg
     4801 ctgggctgcg ggatggtgca tccgaacgtg ctgcgtaacg ttagcatcga cccggaagtt
     4861 tactctggtt tcgccttcgg gatggggatg gagcgtctga ctatgttgcg ttacggcgtc
     4921 accgacctgc gttcattctt cgaaaacgat ctgcgtttcc tcaaacagtt taaataaggc
     4981 aggaatagat tatgaaattc agtgaactgt ggttacgcga atgggtgaac ccggcgatcg
     5041 atagcgatac gctggcgaat caaatcacta tggcgggcct ggaagttgac ggtgtagaac
     5101 cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt gcgcagcatc
     5161 cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg cggcgatcgc ctgctggaca
     5221 tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagct accattggtg
     5281 ctgttctgcc gggtgatttc aaaattaaag cggcgaaact gcgtggcgaa ccgtctgaag
     5341 ggatgctgtg ctccttttct gagctgggaa tttctgacga tcacagtggc attatcgaac
     5401 tgcctgcgga tgcgccgatt ggcaccgaca tccgcgaata cctgaaactc gatgataaca
     5461 ccatcgaaat cagcgtgaca ccaaaccgtg ccgactgctt aggtatcatt ggtgttgcgc
     5521 gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt ccggttggtg
     5581 cgactatcga cgacacgttg ccgattacag tcgaagcgcc ggaagcctgc ccgcgctatc
     5641 ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg atgaaagaaa
     5701 aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc aactatgtac
     5761 tgctcgaact gggccagccg atgcacgctt tcgataaaga tcgcattgaa ggcggcattg
     5821 tggtgcggat ggcaaaagag ggcgaaacgc tggtgctgct tgatggcact gaagcgaagc
     5881 tgaatgctga cactctggtc atcgccgacc acaacaaggc gctggcgatg ggcggcatct
     5941 ttggtggcga acactctggc gtgaatgacg aaacgcaaaa cgtcttgctg gaatgcgctt
     6001 tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat actgatgcgt
     6061 ctcaccgtta tgagcgtggc gttgatccgg tactgcagca caaagcgatg gaacgtgcga
     6121 cccgtctgct gatcgacatc tgcggtggtg aggctggccc ggtaattgat atcaccaacg
     6181 aagcaacgct gccgaagcgt gcaaccatca ctctacgtcg tagcaaactg gatcgcctga
     6241 taggccatca tattgctgat gagcaggtaa ctgacattct gcgtcgtctc ggctgcgaag
     6301 tgaccgaagg caaagacgaa tggcaggcag ttgcgccgag ctggcgtttc gatatggaga
     6361 ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac atcccggatg
     6421 agccggtaca ggcaagcctg attatgggta ctcaccgtga agctgacctg tcgctcaagc
     6481 gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt gatcacctac agcttcgttg
     6541 atccgaaagt gcagcagatg atccatccag gcgttgaagc cttactgctg gcaagcccga
     6601 tctctgttga aatgtcagca atgcgtcttt ctctgtggac cggcctgctg gcaaccgtgg
     6661 tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt ctgcgtttcg
     6721 taccagatac tcaggcaccg ttgggcattc gtcaggatct gatgttagcc ggtgtgattt
     6781 gcggtaaccg ttacgaagag cactggaacc tggcaaaaga gaccgttgat ttctatgatt
     6841 tgaaaggcga ccttgaatcc gttctcgacc tgaccggtaa actgaatgag gttgagttcc
     6901 gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc agcgatttat ctgaaaggtg
     6961 aacgtattgg ttttgttgga gttgttcatc ctgaactgga acgtaaactg gatcttaacg
     7021 gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg gtgcctcagg
     7081 cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg gtcgcagaaa
     7141 acgttcccgc agcggatatt ttatccgaat gtaagaaagt tggcgtaaat caggtagttg
     7201 gcgtaaactt atttgacgtg taccgcggta agggtgttgc ggaggggtat aagagcctcg
     7261 ccataagcct gatcctgcaa gataccagcc gtacactcga agaagaggag attgccgcta
     7321 ccgtcgccaa atgtgtagag gcattaaaag agcgattcca ggcatcattg agggattgaa
     7381 cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt ttgataagct tgggcttagc
     7441 aagcgggatg ccaaagaact ggttgaactg tttttcgaag agatccgtcg cgctctggaa
     7501 aacggcgaac aggtgaaact ctctggtttt ggtaacttcg atctgcgtga taagaatcaa
     7561 cgcccgggac gtaacccgaa aacgggcgag gatattccca ttacagcacg gcgcgtggtg
     7621 accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc caaagacgag
     7681 taatctgatc taactaaaaa ggccgctatg cggccttttt tcttttcact gtcgaagagt
     7741 caccgtaaaa tcaacgccat gacacttcag cagaacggat accatgctga cacttgcccg
     7801 ccaacaacag cgacaaaata ttcgctggtt attatgcctg tcagttttga tgctgctggc
     7861 gcttctctta agcctttgcg ccggtgaaca atggatttcg ccaggtgact ggtttactcc
     7921 tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt acgctggctg tattgctggt
     7981 tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa atcctctggc
     8041 agaacctgga ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg ccgcggtatt
     8101 gcttgggcaa gggcaactcc ccaactgggc gctagggctg tgtgcgattg ctggcgcgct
     8161 tatcatcact ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca gtcggttatt
     8221 gctggctggc gttgcattag ggattatctg cagcgcacta atgacgtggg ctatctactt
     8281 ttccacctct gttgatttac gtcagctgat gtactggatg atgggcggtt ttggcggcgt
     8341 agactggcgg caaagctggc tgatgctggc attgatcccc gtgttgttgt ggatctgttg
     8401 tcagtccagg ccgatgaata tgttagcgct tggcgagatc tcggcgcggc aactgggttt
     8461 acccatgtgg ttctggcgca atgtgctggt ggcagcgacc ggctggatgg ttggcgtcag
     8521 tgtggcgctg gcgggtgcta tcggctttat tggtctggtg atcccacata ttcttcggtt
     8581 gtgtggttta accgatcatc gcgtattact tcccggctgc gcgctggcag gggcgagcgc
     8641 attgctgctg gccgatattg tagcgcgcct ggcattagct gccgcagagc tgcctattgg
     8701 cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa aagcaggacg
     8761 ttagccgcaa aaagacggtc tatgattaaa agctaagatt tttactgacc acacctagga
     8821 gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc
     8881 acgctggaga agtacgccgg taatgtgctg ttaattgtca atgtcgcctc aaagtgtggc
     8941 ttaacgccgc aatatgagca gttggagaat attcaaaaag cctgggccga tcgaggtttt
     9001 gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag cgatgaagag
     9061 attaaaactt actgtaccac cacatggggg gtgacgttcc cgatgttcag taagattgaa
     9121 gttaatggcg aaggacgcca tccgctgtat caaaaattga ttgccgcagc gccgaccaca
     9181 gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggccg tgcaccgctg
     9241 tacccggatg atattttatg gaattttgaa aaattcctgg ttggcaggga cggaaaagtc
     9301 atccagcgtt tttccccgga tatgacgccg gaagatccca ttgtgatgga aagcattaaa
     9361 ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg gaatctaccc
     9421 gcctggggcc gctttcaggc gaggttcggg ctggggaaat cctgcacctg gtggggccga
     9481 atggcgcggg taagagtacc ttactggcgc gaatggccgg aatgaccagc ggtaagggaa
     9541 gcattcagtt cgcggggcaa ccactggaag catggtccgc aacaaaactc gcgctgcatc
     9601 gcgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg cactacctga
     9661 cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg
     9721 ctcttgatga caaactcggt cgtagcacca atcaactttc cggcggtgaa tggcaacgcg
     9781 tacgtcttgc tgcggtggtg ttgcaaatca caccacaagc caatcccgca ggccaattgc
     9841 tgcttcttga tgagccgatg aacagtcttg atgttgcgca acaaagtgcg ttagacaaaa
     9901 ttctgagcgc gctgtgtcag caaggactgg cgattgtgat gagcagtcac gatctcaacc
     9961 acacgttgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg
    10021 gacgcagaga agaggtgctc acgccgccaa atctggcgca ggcctatggg atgaattttc
    10081 gccgtctgga tatcgaaggt cacagaatgc tgatttcgac catctgattt cattagtatc
    10141 ccgaaaatcg ggtaattacg acgctaaatt aatgcaagaa taaaaaacag aggaaccgta
    10201 cggaatgcgt ttctgcctta ttttgatcac agcactgttt ctggccgggt gtagccacca
    10261 taaagcaccg ccgccaaatg ccagactttc tgattcgatt accgttattg ccggtttgaa
    10321 cgaccagcta caaagctggc atggcacgcc gtatcgttat ggtggcatga cgcggcgcgg
    10381 tgtggactgt tcgggatttg tggttgtgac gatgcgcgat cgtttcgatt tgcatctgcc
    10441 ccgtgaaacc aaacaacaag cctctattgg cacgcaaatt gataaagacg agttgctgcc
    10501 tggtgacctg gtctttttca aaacgggttc cggacaaaat ggtttgcatg taggtattta
    10561 cgataccaac aaccaattta tccacgcctc taccagcaag ggagtgatgc gttcctcact
    10621 tgataatgtc tattggcaga aaaatttctg gcaggcgaga cgaatctagt atgtcgaaaa
    10681 attgacggcg atgatacatc gccgtcaatt tactcctgaa tacagtagta ttttgcagac
    10741 aaaaattccg taaatggatt attcgtttat aatcaggata atctaaaaaa aggccccctg
    10801 aaaaagtggt taacatggaa caaatgaaag ttactccttc tgtaattgtt atgtcttatg
    10861 gcctcatccc ttttagccgg atactgaaaa acatccttcg agagggacgg ttaccatgaa
    10921 gatttttttg gagaatcttt atcattctga ttgctatttc ctgccgataa gagataacca
    10981 gcaactcctt gtcggcgttg agttaattac ccatttctcc agtgaagatg gcacggttcg
    11041 tatcccgacg agtcgggtta tagcgcaact taccgaggag caacattggc aattattttc
    11101 agagcagttg gaattactaa aatcatgcca acactttttt attcaacata aactctttgc
    11161 ctggcttaat ttaacgccac aagtggctac attattatta gacagggata attacgcagg
    11221 cgaactatta aaatatccgt ttattgagct gttgattaat gagaattacc cgcatctcaa
    11281 tgaaggaaaa gataatcgcg atcttttgtc cttatcgcaa atgtatcccc ttgtgttagg
    11341 caatttggga gcaggtaata gcacaatgaa agcggttttt gatggtcttt tcacccgcgt
    11401 aatgctggag aaaggtttta ttcagcaaca aatcacgcat cgctccttcg aaccgtttat
    11461 tcgtgcgatc caggcgcaaa tttccccttg ttgcaattgc atcatcgcgg ggggcattga
    11521 tacgccagaa atattggcac agattacccc cttcgatttc catgctctcc agggatgcct
    11581 gtggcctgcc gttcctataa atcagataac gacgttggtt cagcgataac cctcctgttt
    11641 gctggtgttt aagacgagag taaccgtcta cactatcaaa cagataggat ctatgaccct
    11701 gtcttttatt acccgctggc gcgatgaatt gccagaaacc tatacagcac tttcccctac
    11761 gcctttaaat aatgcccggc tgatttggca taataccgaa ctcgctaaca cgctgagtat
    11821 tccatcgtcg ctgtttaaaa atgccgcagg tgtctggggc ggcgaaaccc tactgcctgg
    11881 tatgtcacca ctggcacagg tttacagtgg tcatcagttc gtcgtctggg cgggccaact
    11941 gggtgatggg cgcggcattt tacttggcga acaactgctt gctgatggca ctacaatgga
    12001 ctggcatctg aaaggtgctg gcctgacgcc ttattcgcga atgggtgatg gacgggcggt
    12061 tttacgttcg acgatacgag aaagtctcgc cagtgaggcg atgcattatc tgggcattcc
    12121 gacgacccgc gcgttaagta tcgtcaccag cgattcgcca gtgtatcggg aaacggtgga
    12181 gccaggcgcg atgctgatgc gtgtggcacc aagtcatctg cgctttggtc atttcgaaca
    12241 tttttactat cgccgcgagc cggaaaaggt tcgtcagttg gctgactttg ccattcgtca
    12301 ttactggtca catcttgaag acgatgagga caaataccgt ctctggttta acgatgttgt
    12361 cgcacgtacc gcgtcgttaa ttgcccaatg gcagacggtc ggctttgcac atggggtgat
    12421 gaataccgac aacatgtcgc tgctgggact gacgcttgat tacgggccgt ttggttttct
    12481 tgatgattac gaacccggtt ttatttgtaa tcactcggat catcaagggc gttatagctt
    12541 tgataatcaa cctgctgtcg cgttgtggaa tttacagcgt ctggcgcaga cattgtcacc
    12601 atttgttgcc gtagatgccc tgaatgaggc cctggacagc tatcagcagg ttttgttgac
    12661 gcattatgga caacggatgc ggcagaaact gggcttcatg acggagcaaa aagaggataa
    12721 cgcgctactg aatgaattat tcagtctgat ggcgcgagag cgcagcgatt atacccgcac
    12781 attccgcatg ctgagtctga ccgagcagca cagcgcggcg tcaccgctac gtgaggagtt
    12841 tattgatcgt gcggcatttg atgactggtt tgcccgttat cgggggcgtt tgcaacaaga
    12901 cgaggttagc gatagtgagc gtcagcaact gatgcaaagc gttaacccag ctctggtatt
    12961 gcgcaactgg ttggcgcaac gggcgattga agcggcagaa aagggtgata tgatggaatt
    13021 gcaccgcctg catgaggcgt tgcgaaatcc tttcagcgac agagatgatg actatgtcag
    13081 tcgtccacct gactggggta aacggctgga agtcagctgc tcgagttaaa ggctacttag
    13141 ttaacagcag cttgccagcc tgagttttac ggagcagata ttcttgcccg tcatgatcga
    13201 taatcagttt gccatccgga cctaacagtg tctggctggt aattcgccgg atgggctgcg
    13261 gggaagcggt cttgtgattg gcatccgttt caggcgtgag ttttctgcta tccgtataac
    13321 gcataaaaat taataataat tgatataaca atgattccta ttatcaatat tatggcagac
    13381 cagagcaagc agtaatgtgt tgagaagaca acaagttgcg cagaaatgcc catcccatca
    13441 ggaatgggca cgcattcaga agcgggtatc taccgcaaag gcgagttttt cgaccaggcg
    13501 ttcggtatct tcccagccca gacacgggtc ggtaatggat tgaccgtaag tgagcggctg
    13561 accgccgacg attttttgcg ttccttcgcg caggaaactt tccgccataa ttccagcaat
    13621 ctccgtagag ccattgcgga tttgctgaca aatatcctca caaacttcta actggcgacg
    13681 gtgctgcttc tggcagttac cgtggctgaa atccaccacc agatgttcag gtaaatcaaa
    13741 ctcgtgcagc gtatcgcagg ctgcggcgat atcatcggca tgataattcg gttttttgcc
    13801 gccacgcata ataatgtggc catacgggtt gccgctggtc tgatagatgg tcatctgacc
    13861 atttttgtct ggcgagagga acatatggct ggcgcgggct gcgcggatag catccacggc
    13921 aatccgcgtg ttgccatcgg taccattttt aaaacctacc ggacaggaga gtgctgaagc
    13981 catttcgcgg tggatctgac tttcggtagt acgagcgcca atcgcgcccc aactgattaa
    14041 atcagcaata aactgaccgg ttaccatatc gaggaactca gtcgcggtag ggacacccag
    14101 ctcatttacc tgtaaaagta atttgcgcgc cagctccaga ccgtgattta cccgatagct
    14161 gccgtttaaa tccggatcgg agattagccc tttccagccg acaacagttc gtggtttttc
    14221 aaaataggtg cgcattacga tttccagccg tgactggtac tggttgcgca gcgactgcag
    14281 acgggtggcg tactccattg cagcggtgag atcgtggatc gagcaggggc caataatgac
    14341 caacagtcgc ttatcttcac cattcagtat tttttcaatt ctgcggcggg agtcggtgac
    14401 atgggtggcg acgccaggcg ttacgggata ccgtagcgcg agttcggcgg gcgttaccag
    14461 gctctcaata cgcgcagtac ggagttcgtc agttctgttc attacaagtc tcagaaattt
    14521 gttgcttcta cggcaggact accggaaagt gatgcgcatc acgataaacc aatcgcagct
    14581 gatttcaagt gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa caaaaaaaca
    14641 aaccaatgca ctagttctct agtacattcg gcgactaagg cccatgatat cgaggatctt
    14701 ggtggcaatc tcttctaccg aataattggt actgttaatc cacgggatct gatttttacg
    14761 gtacaaggct tccacttccg cgacttccat cctgcactga cgaagcgagg catagcgact
    14821 gttctcccga cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag tcaggccgaa
    14881 caatttatgc tgaagcggtt tgagcgacgc gggtagcacc agattatcca tatcgtcggc
    14941 aataaagggg tagtttgccg cgcggatacc aaattgcatt gccagataca gactggtggg
    15001 ggttttacca cagcgagaaa caccgagcag gatcacctga gcctgatcca gattgcgcaa
    15061 cgaaatgccg tcatcgtggg cgagggtgta atcaatcgcc gcaatgcgcg catcatattt
    15121 attgagatta ttagggttaa ggccatgggt acgatgagca atcggcgttg gatccagttt
    15181 catctcttgt tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa agccttcact
    15241 ttgcaagatg atggcgcgaa tctccggcaa cacgatggag tagaagacca gcgggcgcac
    15301 gcctgtctgg tgataaattg cgtcaatctg atccttcact gcccgtgcac ggctctcatt
    15361 ttcgacaaac ggcagcgtga tgctgctgat agtgacggga aattgtgaca ttactgcgtg
    15421 tcctaatacc tccgcagtta ttgccgtacc atcagaaata taaaaaacgt ggcgatcaac
    15481 agcattatcc attttgttct tcccgtgaag cagacataat tctatgaaag cataaattaa
    15541 aagcgcacag aagcgtagga cgtaatgtct ggtttataaa atgaaccttc attttatttt
    15601 ttataaaaac agcatttcat ttttatggtt tcgtttatac cgatggttta tgtggaaatt
    15661 gtcgaataga gcaggtttgc gcaacgctgg gatcagtctt aaaaagtaaa aaaatatatt
    15721 tgcttgaacg attcaccgtt tttttcatcc ggttaaatat gcaaagataa atgcgcagaa
    15781 atgtgtttct caaaccgttc atttatcaca aaaggattgt tcgatgtcca acaatggctc
    15841 gtcaccgctg gtgctttggt ataaccaact cggcatgaat gatgtagaca gggttggggg
    15901 caaaaatgcc tccctgggtg aaatgattac taatctttcc ggaatgggtg tttccgttcc
    15961 gaatggtttc gccacaaccg ccgacgcgtt taaccagttt ctggaccaaa gcggcgtaaa
    16021 ccagcgcatt tatgaactgc tggataaaac ggatattgac gatgttactc agcttgcgaa
    16081 agcgggcgcg caaatccgcc agtggattat cgacactccc ttccagcctg agctggaaaa
    16141 cgccatacgc gaagcctatg cacagctttc cgccgatgac gaaaacgcct cttttgcggt
    16201 acgctcctcc gccaccgcag aagacatgcc ggacgcttcc tttgccggtc agcaggaaac
    16261 cttcctcaac gttcagggtt ttgacgccgt tctcgtggca gtgaaacatg tatttgcttc
    16321 tctgtttaac gatcgcgcca tctcttatcg tgtgcaccag ggttacgatc accgtggcgt
    16381 ggcgctctcc gccggtgttc agcggatggt gcgctccgac ctcgcatctt ctggcgtgat
    16441 gttctccatt gataccgaat ccggctttga ccaggtggtg tttatcactt ccgcatgggg
    16501 ccttggggaa atggtcgtgc agggtgcggt taacccggat gagttttacg tgcataaacc
    16561 gacactggcg gcgaatcgcc cggctatcgt gcgccgcacc atggggtcga aaaaaatccg
    16621 catggtttac gcgccgaccc aggagcacgg caagcaggtt aaaatcgaag acgtaccgca
    16681 ggaacagcgt gacatcttct cgctgaccaa cgaagaagtg caggaactgg caaaacaggc
    16741 cgtacaaatt gagaaacact acggtcgccc aatggatatt gagtgggcga aagatggcca
    16801 caccggcaaa ctgttcattg tgcaggcgcg tccggaaacc gtgcgctcac gcggtcaggt
    16861 catggagcgt tatacgctgc attcacaggg taagattatc gccgaaggcc gtgctatcgg
    16921 tcatcgcatc ggcgcgggtc cggtgaaagt catccatgac atcagcgaaa tgaaccgcat
    16981 cgaacctggc gacgtgctgg ttactgacat gaccgacccg gactgggaac cgatcatgaa
    17041 gaaagcatct gccatcgtca ccaaccgtgg cggtcgtacc tgtcacgcgg cgatcatcgc
    17101 tcgtgaactg ggcattccgg cggtagtggg ctgtggagat gcaacagaac ggatgaaaga
    17161 cggtgagaac gtcactgttt cttgtgccga aggtgatacc ggttacgtct atgcggagtt
    17221 gctggaattt agcgtgaaaa gctccagcgt agaaacgatg ccggatctgc cgttgaaagt
    17281 gatgatgaac gtcggtaacc cggaccgtgc tttcgacttc gcctgcctgc cgagcgaagg
    17341 cgtgggcctt gcgcgtctgg aatttatcat caaccgtatg attggcgtcc acccacgcgc
    17401 actgcttgag tttgacgatc aggaaccgca gttgcaaaac gaaatccgcg agatgatgaa
    17461 aggttttgat tctccgcgtg aattttacgt tggtcgtctg actgaaggga tcgcgacgct
    17521 gggtgccgcg ttttatccga agcgcgtcat tgtccgtctc tctgatttta aatcgaacga
    17581 atatgccaac ctggtcggtg gtgagcgtta cgagccagat gaagagaacc cgatgctcgg
    17641 cttccgtggt gcgggccgct atgtttccga cagcttccgc gactgcttcg cactggagtg
    17701 tgaagcagtg aaacgtgtgc gcaacgacat ggggctgacc aacgttgaga tcatgatccc
    17761 gttcgtgcgt accgtagatc aggcgaaagc ggtggttgaa gaactggcgc gtcaggggct
    17821 gaaacgtggc gagaacgggc tgaaaatcat catgatgtgt gaaatcccgt ccaacgcctt
    17881 gctggccgag cagttcctcg aatatttcga cggcttctca attggctcaa acgatatgac
    17941 gcagctggcg ctcggtctgg accgtgactc cggcgtggtg tctgaattgt tcgatgagcg
    18001 caacgatgcg gtgaaagcac tgctgtcgat ggctatccgt gccgcgaaga aacagggcaa
    18061 atatgtcggg atttgcggtc agggtccgtc cgaccacgaa gactttgccg catggttgat
    18121 ggaagagggg atcgatagcc tgtctctgaa cccggacacc gtggtgcaaa cctggttaag
    18181 cctggctgaa ctgaagaaat aaaataaatc cccggcggcg ttcagtcgcc ggggttatgt
    18241 gatccccgaa gatgaaactt attcaatctc ttcacagaca tcctgcgtta atcgccgtat
    18301 aatatctttt cttaacaaaa acttttgtat tttatctgag gcagtgcgcg gtagtttttc
    18361 gattaccacg atatgttcag gatatttata ttttgcgacc cgtttacggc taaaaaaagc
    18421 cgccatctct tccagcgata atgaatgatg cggcgctttc agcacgacat aagcgcatga
    18481 tcgttcacct aaacgttcat cgggcattgc aaccacacag gcatcgtgaa ttttaggatg
    18541 ctgcaataaa atatcttcca cttcacggct gctaatattt tcgccgccgc ggacaataat
    18601 atcttttttg cgtccggtaa tttttatata gccagcctca tccatacggc agagatcgcc
    18661 gctgtaatac cagccttctt catccagcgc acgggcggtt aattcaggtt catcaaaata
    18721 ccccataaac acattggggc cacgcgatgc ttcttcacct tcgcaacctg gcggtaaggt
    18781 cttgcgtgcg tcatcgacca ctttaatctc tacacctgcg gcagcgtaac catcggtatg
    18841 cataaagcgc gacaaagaat tatcgagatt caccaccgca tgcggcgaac tttctgtgga
    18901 accataaaca cttaataatt taatgctgcg ctgctggcat tcgcgcgcca cttttttggg
    18961 gattgtggta ccgccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gttgtttctc
    19021 tagtaaattc aaaagatcat agacaaacgg cgttgcgcct agcatacagg tgcaacgctg
    19081 ctgctcaagc agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc
    19141 gccaattaag aatggtgccg ttacgccatg cagaaagccc gttgcgtgac caagtggcgc
    19201 aggcatcata aagacatcca gccaggtcag attcagccgc gcgcaataag cccgctcact
    19261 ggcgagaata ttgttatgcg ttagcatcac gccctttggc agaccctcgg ttccggaggt
    19321 aaacagcacc gcagctaatt catcgccgtg ggtcgttatc gccgtggtca gtgggatatt
    19381 gtcggcgata atctgactta atgagaggga agaggtggcg ggagccagtt tgtccacgcc
    19441 gacaatttgt tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccggacg
    19501 cgtttgttta aacaacgtcg gtgcaaagaa catttttgcc tgacacttat tgagcaccca
    19561 caccagttct gcttcccgcc aggaaggcaa cagcggcacg gaaaccgcac cgattttcag
    19621 gcaggcaaga tagataacgg taaattcaca ccagccaggc agttgaaatg cgatgcgatc
    19681 gcctgattca atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag
    19741 cgcgctatag gtgtacgatg caccatgatt atcgaccacg gcaattttgt ctggcatcgc
    19801 acgagcggtc tgctgccagt aatcggccag tgaagcatcg ccccatagcc cttgctgacg
    19861 atacgccgca cgacgttgtt cgttaaacgt taatgtcact ttcattttgg actctcgaaa
    19921 cagaacatca ggcccgagtt gcgggcctgt gggatgcatg attagccgta gcgaaaatca
    19981 ataccgaagg tgcctgcggg atattgccac tgttcgagaa tagtgttacc gcacagcacc
    20041 cgacaggtgc cgcactccag acaaccggcg ggatcaaaat gaatgtttcc tacgtcatcc
    20101 tgcttataaa gtccggcagg gcaggctttc attaatttac ggaattcatt gatatcggga
    20161 ttttccgtca gaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat
    20221 ttgatgtcga cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat
    20281 ctttcagcaa gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg
    20341 gctggtttgg tttgccgtca atggtgaaca tctcgttcat gatgtcggct accattcgtg
    20401 ggtattggct aaacaggcgc gggttttcca tcagcgccgg gatcttgcga aaatgctgca
    20461 tatcacgcat gacgcagctt tgttccagct cacgtttgta ttgcgccaga ctgttggcgg
    20521 agaaatccgc gcgttctttg gcggcgatca ccgttgtggc ggcagcctga gccgatgcaa
    20581 tggctaaatc catgccgcgg accgtaaaac ccaaattcag gcagaagcct gcggcgtcac
    20641 caacgatcat cacgccctcg ttaaccagtt gcggcaccat tgccagaccg ccttccggca
    20701 ccatatgcgc ggaatattca agcagtttgc cgccgctaat cagcgggcga atggcggggt
    20761 gttgtttaaa atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcaccca
    20821 atccacaaac cagccccaag gatatggaat ccttgttggt atagagaaat cccccgccca
    20881 tcaggccgtc agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaatat
    20941 taaagcgatc gttgatctgt tctggtgtga ggccaataac ctctttaaca ccaacagcgt
    21001 aatgatgcgg atcggaagcg ggaaccattc ccagcgagcg gccaagcatc gagttaacgc
    21061 catcagccag aatcaccaca ttcgcttcga gaatatcatc cccagcctgc acgccagtga
    21121 ccttgtttcc ttcacgaacc agcgcgtcga cgcgaacgcc tgggataaac tgcgcgcccg
    21181 cctgctcggc ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagatg
    21241 cgtgttgagg aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg ctttcttcgg
    21301 ttaagaagga gattttctcg cgtgtgacct tgcgttctac cggcgctgat actgcaaagc
    21361 ctggaatgat tgcttcaagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc
    21421 cggcactgtc gccgcgttct atcaccagga catccagccc ggctcgtgcc atgaccagtg
    21481 cggcaacgct accagcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca
    21541 tgcgttatgc tcccttcctg ctgcgtttac agcgggagat gtggttgaaa ggggaattag
    21601 cggctcaact ggctaatcag ggcagggacg actttgtaga tatcgcccac cagaccgtag
    21661 tcggcatagt tgaagattgg cgcattttta tctttattga tggcgacaat cacttttacg
    21721 ccgttgccgc caaccatatg ttggatctgc ccggagatcc ccagcgtcag gtagagatcg
    21781 gatttcagca acacgccgga gacaccgata taacgttcac gctccatcca gttctcgcct
    21841 tcggcaattg gacgtgaaca gccgacttca gcattcagca ccgccgccag ttcgtggacc
    21901 attttcaggt catcctgcgc cgccagacca cgaccgacgc caaccacacg ttttgctttg
    21961 ctcaggtcca cgctgcttgc ggctttcgca cggcgttcgc gacagagaat ttcatgacgc
    22021 ggagcaacat aaggtaccgt ttctgtcggg cactgatgag aggtatcact ggtgcacggt
    22081 tcctgaacac cgggtgcaag ggtaatgatc gccagcgggc tgttgatctt ttcctgagcg
    22141 aacgctaacc cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac aatatccacc
    22201 gccgtggcat cgttcaccag cgccgcattc agttgcacac ttaaccgtgc tgccagagct
    22261 ttaccacgtt tcgtcgcggc caacagcaac atagcggggt gtttatcttt cagcagggca
    22321 gcaatgcttt cggcgtaatt ttcagtgcgt tgcagcgcgt cgttttgctc aagaacataa
    22381 atacattttg gaccataagg cataactgcc tgcgcctggt cggtattttg tacaatggca
    22441 tacacctgtt ggccccattg ctgagcgccg ccaaacagtt cagcataacg ttcaggatta
    22501 tcgctaaaga cccagacgct gtttaattga ctcatcgttt cttctccgta ggcttcagtt
    22561 cagggctttc ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat cgttatcgag
    22621 aatgatgtgc ttacgttctg tttgcggcgg tacgcgaatg ccgaccagtt cagcaagtgg
    22681 cgcgctctgg ctccagtcaa tatcacttgc ctgccactga tttaccggtt ttttacccgc
    22741 gccgagaatg gctttcatcg aaggaatgcg tggcacgtta atatcggagg tgacgcagag
    22801 cacggctgga acagagagtt caataacttc aacatcatct tcaagcgtgc gttcaatcac
    22861 cagtgtattg ccctgacgct gaatagcact cactgcatta atcaccggaa gttgcagaat
    22921 ttctccgacc agcaagccaa cctgctgggc ataaaggtcg ccggaacctt caccaaagat
    22981 cagtaaatcg aagccgatct tttcaattgc tgccgccagc gcttttgcgg tatcgagagg
    23041 cagtgcatgt tcaagttgcg catcctgcac caaatacagg ctgtgcggcc cgcgggatag
    23101 cacgtctttg cgcactttcg agttctgcaa caatgagccg ccaacggtca gcgcggctat
    23161 ctcatcgtca tctgttgcga gctggcttgc agcttcaatg gcattgagat cgaactggct
    23221 gattttggcg tcggcattgt cgaaattcag ggtgtattct ggagtgacaa caatgtcctg
    23281 ttcttcaggc accagcttaa agcaggttat tattttcatg atttctcctg tgagtcgcat
    23341 acggaaccat ctccgatgga aggtaaaaat ggcctgaaaa tagtgtgcga tagcggtgcg
    23401 gggggggggg cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga
    23461 ttgctaaaac aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc
    23521 attcagtgta aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac
    23581 gccgtatttt atccggcata tattgttttt gcttgctcat ggaataggga ggttgtca
//
LOCUS       DAJTYL010000060        22546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYL010000060 DAJTYL010000000
VERSION     DAJTYL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.060"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..542
                     /locus_tag="OO572_002301"
     CDS             <1..542
                     /locus_tag="OO572_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6661283.1"
                     /translation="QQAADNNMEFANYGPFELLQARQLIESNIAEFAATQVTKQDIMK
                     LMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALAAIVEKMWTQRSHNPYWKKLHE
                     HIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNETSDDFEFNADR
                     YLFAENPVVHLDTATSGSK"
     gene            887..1549
                     /locus_tag="OO572_002302"
     CDS             887..1549
                     /locus_tag="OO572_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6661284.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1553..1936
                     /gene="mzrA"
                     /locus_tag="OO572_002303"
     CDS             1553..1936
                     /gene="mzrA"
                     /locus_tag="OO572_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6661285.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTLPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2083..2437
                     /locus_tag="OO572_002304"
                     /pseudo
     CDS             2083..2437
                     /locus_tag="OO572_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2475..2780
                     /locus_tag="OO572_002305"
     CDS             2475..2780
                     /locus_tag="OO572_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6661286.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2783..3187
                     /locus_tag="OO572_002306"
     CDS             2783..3187
                     /locus_tag="OO572_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6661287.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3177..3476
                     /locus_tag="OO572_002307"
     CDS             3177..3476
                     /locus_tag="OO572_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6661288.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3662..3958
                     /locus_tag="OO572_002308"
     CDS             3662..3958
                     /locus_tag="OO572_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6661289.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4028..5014
                     /gene="yqjG"
                     /locus_tag="OO572_002309"
     CDS             4028..5014
                     /gene="yqjG"
                     /locus_tag="OO572_002309"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6661290.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5307..5675
                     /locus_tag="OO572_002310"
     CDS             5307..5675
                     /locus_tag="OO572_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6661291.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(5882..6778)
                     /gene="yhaJ"
                     /locus_tag="OO572_002311"
     CDS             complement(5882..6778)
                     /gene="yhaJ"
                     /locus_tag="OO572_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6661292.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            6883..7584
                     /locus_tag="OO572_002312"
     CDS             6883..7584
                     /locus_tag="OO572_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6661293.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7607..7771
                     /locus_tag="OO572_002313"
     CDS             7607..7771
                     /locus_tag="OO572_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661294.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(7983..9293)
                     /locus_tag="OO572_002314"
     CDS             complement(7983..9293)
                     /locus_tag="OO572_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6661295.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9321..10652)
                     /locus_tag="OO572_002315"
     CDS             complement(9321..10652)
                     /locus_tag="OO572_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6661296.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(10927..12291)
                     /gene="tdcG"
                     /locus_tag="OO572_002316"
     CDS             complement(10927..12291)
                     /gene="tdcG"
                     /locus_tag="OO572_002316"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6661297.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12363..12752)
                     /locus_tag="OO572_002317"
     CDS             complement(12363..12752)
                     /locus_tag="OO572_002317"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6661298.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(12766..15060)
                     /gene="tdcE"
                     /locus_tag="OO572_002318"
     CDS             complement(12766..15060)
                     /gene="tdcE"
                     /locus_tag="OO572_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6661299.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15094..16302)
                     /gene="tdcD"
                     /locus_tag="OO572_002319"
     CDS             complement(15094..16302)
                     /gene="tdcD"
                     /locus_tag="OO572_002319"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6661300.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16328..17659)
                     /gene="tdcC"
                     /locus_tag="OO572_002320"
     CDS             complement(16328..17659)
                     /gene="tdcC"
                     /locus_tag="OO572_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6661301.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17681..18670)
                     /gene="tdcB"
                     /locus_tag="OO572_002321"
     CDS             complement(17681..18670)
                     /gene="tdcB"
                     /locus_tag="OO572_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6661302.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18769..19707)
                     /gene="tdcA"
                     /locus_tag="OO572_002322"
     CDS             complement(18769..19707)
                     /gene="tdcA"
                     /locus_tag="OO572_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6661303.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            19897..20241
                     /gene="tdcR"
                     /locus_tag="OO572_002323"
     CDS             19897..20241
                     /gene="tdcR"
                     /locus_tag="OO572_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6661304.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20497..21037
                     /locus_tag="OO572_002324"
                     /pseudo
     CDS             20497..21037
                     /locus_tag="OO572_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21059..21406
                     /locus_tag="OO572_002325"
     CDS             21059..21406
                     /locus_tag="OO572_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661305.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21575..22225
                     /locus_tag="OO572_002326"
     CDS             21575..22225
                     /locus_tag="OO572_002326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661306.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6080 a         5691 c         5192 g         5583 t
ORIGIN      
        1 atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc
       61 aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac
      121 aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg
      181 attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc
      241 tggcggccat cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac
      301 tacacgaaca cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc
      361 tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg cagcatctgg
      421 aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc
      481 gctatctgtt cgccgaaaac ccggtagtgc atctcgatac ggccaccagc ggcagcaaat
      541 gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt ctcgtcggta agcgaaaagt
      601 ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcaatcag caaaatcaga
      661 ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa
      721 tttgaattta tgcttttgaa tgctttctta tctcacgttt taacagggaa tagttcaggc
      781 tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca
      841 tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac
      901 ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat
      961 tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaatggct tgcttccggc
     1021 ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc
     1081 gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gttgctgggt
     1141 cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca
     1201 tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc
     1261 gctgttaatt ggtcgcttta ttgcatttgt cagaacactg ctgccgacga ttgccgggtt
     1321 atcagggctg aataacgcgc gctttcagtt cttcaactgg atgagcggtc tgctgtgggt
     1381 attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt taaagtacga
     1441 ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc
     1501 aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat
     1561 acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac
     1621 gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt
     1681 tcatcaaggc acaacgctgc cagacggttt ttcgatctgg catcaccttg acgctcatgg
     1741 cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag
     1801 cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat
     1861 tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc
     1921 tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc
     1981 gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc
     2041 gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct
     2101 ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa
     2161 aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt
     2221 attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg
     2281 cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa
     2341 gatttagccg aggcgaagca aaaaggcgat gccgatagaa cggaaactgg cagaagcgca
     2401 ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca ctacttactc
     2461 acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct gagttgaaat
     2521 ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg aaagaagagt
     2581 tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat cgcctgggtg
     2641 aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct gatgagtatg
     2701 tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta gtgctcggcg
     2761 ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc ccggtaaaag
     2821 cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg aaacacgtct
     2881 gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac ttttactgat
     2941 gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg tgctaattat
     3001 ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg tggtgttgct
     3061 gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat ccaccttgct
     3121 gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg agtcccgtga
     3181 gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc cagcaacaac
     3241 ggctggatat ttccgccagt agtcgtgaat ggctggaggc aacaggcgct tacgatcgtc
     3301 gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtaga gtgatggcga
     3361 tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt tttggcgtat
     3421 ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc ggttaacttc
     3481 cctctggccg gagcaattcc ggccttatcc atcaaatttt ttgaatattt ttgacagttt
     3541 tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca gcaacgacgc
     3601 taatagcgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag tggagagtat
     3661 gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac attttaatgc cgattctgtt
     3721 tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat atatggaagc
     3781 aatgggtgtc ccgggtttta tgctgctgac ggcattttta tttcacagca actttgctga
     3841 aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct
     3901 ggcaattacc ggtccgggcg cgtatagcat cggccgcctg ctgaataaaa agtggtaagc
     3961 acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga
     4021 gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc
     4081 taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg
     4141 cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta
     4201 tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact
     4261 ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt
     4321 tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca
     4381 gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg tgctgtggga
     4441 caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca tgttcaatac
     4501 cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc tgcaaacgaa
     4561 aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg
     4621 ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc
     4681 gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc
     4741 cgatattcgc ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga cccacttcaa
     4801 gtgtgataag caccgcatca gcgattacct gaatctgtat ggcttcctgc gcgatatcta
     4861 ccagatgccg gggatcgccg aaactgtcaa tttcgatcac atccgtaatc attacttccg
     4921 cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc aggatctcga
     4981 tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg aggtgcctct
     5041 gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct tatcaaacct
     5101 acaaagcgtt tatattcaat atgttacaat gaccgcgtag gccagataag cgtagcgcat
     5161 ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc acggcacttt attaattcct
     5221 tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc tttaaaaaca
     5281 agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta aaaaattatg
     5341 tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg gtcaacatca
     5401 tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc gctggtggcg
     5461 aaggcatcct gacgataatc tacggtattc tggtgttttt accgtggtgg gcggttcagt
     5521 tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgctattc ttaatcccgt
     5581 ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca ggcgaaaacc
     5641 gctttggacc agatccaaaa ctggaacaag aataatgatt aagcaccttg taaaaggtgc
     5701 ttcaaattgt tgacaaagtg cgctttgttt atgccggatg cggcgtaaac gccttatcca
     5761 gcctacaaat tgccaaaatt caatatattg cagtgactgc gtaggcctga taagcgtagc
     5821 gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc ttgtgaaagg tgcttaatag
     5881 attattttcc agcgaacaac tttggaattt cccgcagaca ccaggatttc gcttccccca
     5941 tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga
     6001 cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc
     6061 caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct
     6121 gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa
     6181 ttccacgata tttcacacgc gtcacttcag ataacggttc cggctcctga tgaatcgggt
     6241 gatcaggcgc ggcaacgtag acgttcatta acgtatagag cttgcgcgag ttgatctccg
     6301 acgaggaacg aaaatgcata tccggcgcga taacgatatc cgcccgcccc tgctccagcc
     6361 gttcccacgc ccccgccagc acttctgtga tgattgccag ttgggtattg gcttttgccg
     6421 ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg
     6481 tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc agtagtcagt ttatctgcgg
     6541 cttccagcaa aacgcgccct cgctccagca acatcctccc gacattggtg aatttggtgc
     6601 gatggcccga gcggtcaaac agcaccacat ccagctcttc ttccagtttt tgcatggtgt
     6661 agctaagtgc ggaaggcacg cgtcccagct catccgctgc cgccgcaaaa ctgccccggc
     6721 gatcgatcgc atccataacc cgtagtgctt ccagcgttaa tgccctttct ttggccattt
     6781 cgttctcatt caggaaattt gaacataccg ggcagaatat ctggctaaca atgtagcgtc
     6841 cagcccctta ccataaaagg aagtaaagag aggtcaagaa ttatgattac tacccgaact
     6901 gccaggcagt gtggacaagc agactacgga tggttgcagg cccggtatac tttttccttt
     6961 ggacactact tcgacccgaa attgttaggc tatgcctccc tgcgtgtgct taaccaggaa
     7021 gtgctggccc caggtgccgc ctttcagccg cgaacctatc ccaaagtcga tattttaaat
     7081 gtgattctgg atggagaagc agagtatcgc gacagcgaag gcaatcatgt tcaggccagc
     7141 gccggagagg cgttgctgct ctctacccag ccgggtgtca gctatagcga acacaatctc
     7201 agcaaagaca aaccgttaac gcgaatgcag ctttggctgg atacctgccc gcagcgagag
     7261 aatccgctga ttcaaaagct ggcgcttaat atggacaagc agcaattaat cgcctcgcca
     7321 gagggtgcga tgggaagcct gcaattacgc caacaagtgt ggctgcacca tatcgtgctc
     7381 gacaaaggcg aaagtgcgaa tttccagttg catgggccac gcgcgtattt gcaatcgatt
     7441 cacgggaaat ttcatgcgct tacgcatcat gaagagaaag cggcgctaac ctgcggtgat
     7501 ggggcgttta ttcgtgacga ggctaacatt acgctggttg ccgattcccc actgcgcgct
     7561 ttgctgatag atttgcctgt ctagttgttt ttacagggag atgatgatga gtaaaaaatc
     7621 gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa
     7681 aaattttggc tatgaagaga tgttgagcga gctggaagct atcgtcgcgg atgctgaaac
     7741 gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt gccggatgcg gcgtaaacgc
     7801 cttatccggc ctacaaaaca caacaacatc aacaaattaa acaccccgta ggccggataa
     7861 gatgcgccag catcgcatcc ggcatcaggt acaaaacaca acaacatcaa caagttgagc
     7921 accccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc
     7981 gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa
     8041 tggcttgcta acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct
     8101 tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa
     8161 gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg
     8221 acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc
     8281 catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa
     8341 cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc
     8401 agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat tattgcagtg
     8461 atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc
     8521 gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa
     8581 ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa
     8641 ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca
     8701 aatgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc
     8761 agcggcgact cttgctcgcc ctctgccaca cacgcctggt gggtaaacac cacaccattg
     8821 tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc
     8881 tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta
     8941 acagagactt tccccgccgc cagcagtgct ttggcatcgg taattgcctg cgcagttgcg
     9001 tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca
     9061 atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga
     9121 tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc
     9181 gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc
     9241 tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa
     9301 accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca aagcaacaaa
     9361 ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg
     9421 taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct acagatagag
     9481 gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa aatggcgaaa
     9541 atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc
     9601 aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat
     9661 gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg
     9721 tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt
     9781 accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca
     9841 aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaatt
     9901 gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat aaacagaatc
     9961 gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt
    10021 agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag cgcagcgact
    10081 accagcagct tggtcagcac catgtcggtc gaaattttga ataacaattt ctcgccgcgt
    10141 gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc
    10201 aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg
    10261 gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa caggatgccc
    10321 cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct
    10381 gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat
    10441 gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc
    10501 gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg
    10561 atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct
    10621 gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct
    10681 tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt
    10741 attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttaa
    10801 aataacggag atggataaga attttctact aaattaatca caagagaata ttcattctct
    10861 atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca
    10921 gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt
    10981 atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg
    11041 cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca cggcattaat
    11101 ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc
    11161 aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag
    11221 tagttcagtt aaccctgccg ctgccattga acaggccacg ccaatctccc cctgacagcc
    11281 gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc
    11341 cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc
    11401 cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc
    11461 agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat
    11521 cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgaca
    11581 cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc
    11641 atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc
    11701 cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag
    11761 cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc
    11821 gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag
    11881 cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc
    11941 gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac
    12001 tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac
    12061 atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt
    12121 gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg cggttaataa
    12181 gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg
    12241 cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc
    12301 ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg
    12361 ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc
    12421 gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta
    12481 tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg
    12541 atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa
    12601 cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc
    12661 ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct
    12721 ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt
    12781 aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc
    12841 gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag
    12901 cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc
    12961 gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt
    13021 gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa
    13081 cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat
    13141 cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt
    13201 tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt
    13261 gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat
    13321 gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc
    13381 caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag
    13441 ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg
    13501 atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat
    13561 attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat
    13621 cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat
    13681 cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg ccaggttagc
    13741 gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc agcaggcaat
    13801 cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat actgcaagga
    13861 agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga
    13921 ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata
    13981 gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc
    14041 cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg
    14101 taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag
    14161 gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg tgcgccccag
    14221 cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt agagccactg
    14281 caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat
    14341 ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt
    14401 ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg
    14461 ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg
    14521 cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat
    14581 atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag
    14641 atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt
    14701 aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat
    14761 tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt
    14821 ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacctt
    14881 ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat cgccttcata
    14941 cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc agtccgtacc
    15001 tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat ctaccttcat
    15061 gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac
    15121 tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacgac
    15181 gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt tagaacgatt
    15241 attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg
    15301 aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca gccgacgtaa
    15361 tgaagctgca tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag
    15421 ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag
    15481 accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct
    15541 ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt
    15601 acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac tctgaccgtt
    15661 gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc
    15721 ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg
    15781 gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata
    15841 agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc
    15901 cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat gcaggggtgc
    15961 cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt
    16021 aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt catttaaatt
    16081 ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag
    16141 cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc
    16201 aatacctgac attaatactt cacaatcgct ggcatcgagt acggaaaact taatcgaaga
    16261 cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag
    16321 ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa
    16381 caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta cggatagcat
    16441 acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt
    16501 cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc
    16561 tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca
    16621 gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga
    16681 tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttcg
    16741 taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt
    16801 tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca
    16861 ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga
    16921 agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga
    16981 ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga
    17041 taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt
    17101 tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca
    17161 ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca
    17221 gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc tggttttccc
    17281 agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg
    17341 cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg
    17401 tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc agcgcccggt
    17461 ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc
    17521 caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc
    17581 ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa
    17641 tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg
    17701 tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc
    17761 tattttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg
    17821 cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt
    17881 cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac
    17941 ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt
    18001 ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg ccaataacac
    18061 gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccaccaccac
    18121 caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg
    18181 ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata cgaccttcca
    18241 tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga
    18301 cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca
    18361 ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc
    18421 ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat
    18481 taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata
    18541 tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc
    18601 gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc agatcgtatg
    18661 taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac
    18721 ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaacaact aaccaacttc
    18781 tattaattgc cttcgtctac acccattata agatgaatac tctttggcta attccaccaa
    18841 aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac
    18901 aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat
    18961 atcacaagga attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac
    19021 tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag
    19081 ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc
    19141 cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc
    19201 gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc
    19261 aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat
    19321 agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt
    19381 aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcttcag aagacatacc
    19441 gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa
    19501 caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc
    19561 aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc
    19621 ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg
    19681 cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat
    19741 gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt aactttcatt
    19801 actttccaag gtatcgttat tacaattaaa catctttaaa tatcaacaag ttaaagcata
    19861 aaaatcagca taaaccctga tttttatcaa aaaaaaatga cggggataac catattttat
    19921 ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc atatttcaag
    19981 caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa gttctcaaat
    20041 tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat tttttcacgc
    20101 tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa tacaggtatt
    20161 gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac gtcattttgt
    20221 gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag cattttgtgt
    20281 tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga agtcaacaag
    20341 ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc gtttactgta
    20401 acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga aaataagcat
    20461 ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc aattgcacat
    20521 atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa tagaaatatt
    20581 gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga cgttaataaa
    20641 atttacagta taagtaatga actccggaga gtattatctg caattactgc attgaatttc
    20701 tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa ccggaaaata tgagtccatt
    20761 catcatagat atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg
    20821 cacttttgca ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga
    20881 atgtattaag gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt
    20941 aacaccccct aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt
    21001 tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat
    21061 gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa
    21121 tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa
    21181 actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa
    21241 atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta
    21301 taatgactcg tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc
    21361 ggacaatacc tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg
    21421 caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt
    21481 aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt
    21541 cgatttagat tattatgatt tcacagataa acatatggct aatactattc taaatcattt
    21601 taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc
    21661 aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag
    21721 cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct
    21781 tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc
    21841 aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc
    21901 atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc
    21961 aaaaaaaaca tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa
    22021 tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag
    22081 ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc
    22141 ggggaatata acacatatga agcatataac tcagagaatc aatattttac cagagaggac
    22201 tatacgtata attacgacct tttaaatgca atatagactt aataacttct tacaaagtct
    22261 attattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg
    22321 cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac
    22381 gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca
    22441 gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg
    22501 tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatag
//
LOCUS       DAJTYL010000061        22433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYL010000061 DAJTYL010000000
VERSION     DAJTYL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.061"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..489
                     /gene="yeiP"
                     /locus_tag="OO572_002327"
     CDS             <1..489
                     /gene="yeiP"
                     /locus_tag="OO572_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6661307.1"
                     /translation="SPTARGAATLYKMRFSDVRTGLKVEERFKGDDIVDTVTLTRRYV
                     DFSYVDGNEYVFMDKEDYTPYTFTKDQIEEELLFMPEGGMPDMQVLTWDGQLLALELP
                     QTVDLEIVETAPGIKGASASARNKPATLSTGLVIQVPEYLSPGEKIRIHIEERRYMGR
                     AD"
     gene            712..2178
                     /locus_tag="OO572_002328"
     CDS             712..2178
                     /locus_tag="OO572_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6661308.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2296..3282
                     /gene="yeiR"
                     /locus_tag="OO572_002329"
     CDS             2296..3282
                     /gene="yeiR"
                     /locus_tag="OO572_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6661309.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3321..4034
                     /gene="lpxT"
                     /locus_tag="OO572_002330"
     CDS             3321..4034
                     /gene="lpxT"
                     /locus_tag="OO572_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6661310.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4446..5012
                     /gene="mepS"
                     /locus_tag="OO572_002331"
     CDS             4446..5012
                     /gene="mepS"
                     /locus_tag="OO572_002331"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6661311.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5193..6749
                     /locus_tag="OO572_002332"
     CDS             5193..6749
                     /locus_tag="OO572_002332"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6661312.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6831..8645
                     /locus_tag="OO572_002333"
     CDS             6831..8645
                     /locus_tag="OO572_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6661313.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8646..9740
                     /locus_tag="OO572_002334"
     CDS             8646..9740
                     /locus_tag="OO572_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6661314.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9740..10765
                     /locus_tag="OO572_002335"
     CDS             9740..10765
                     /locus_tag="OO572_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6661315.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10767..12356
                     /gene="yejF"
                     /locus_tag="OO572_002336"
     CDS             10767..12356
                     /gene="yejF"
                     /locus_tag="OO572_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6661316.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12360..12704)
                     /locus_tag="OO572_002337"
     CDS             complement(12360..12704)
                     /locus_tag="OO572_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661317.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13037..14227)
                     /gene="bcr"
                     /locus_tag="OO572_002338"
     CDS             complement(13037..14227)
                     /gene="bcr"
                     /locus_tag="OO572_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6661318.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14255..14950)
                     /gene="rsuA"
                     /locus_tag="OO572_002339"
     CDS             complement(14255..14950)
                     /gene="rsuA"
                     /locus_tag="OO572_002339"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6661319.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15100..16860
                     /locus_tag="OO572_002340"
     CDS             15100..16860
                     /locus_tag="OO572_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6661320.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            16985..17269
                     /gene="rplY"
                     /locus_tag="OO572_002341"
     CDS             16985..17269
                     /gene="rplY"
                     /locus_tag="OO572_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6661321.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17408..18415)
                     /gene="yejK"
                     /locus_tag="OO572_002342"
     CDS             complement(17408..18415)
                     /gene="yejK"
                     /locus_tag="OO572_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6661322.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18597..18824
                     /locus_tag="OO572_002343"
     CDS             18597..18824
                     /locus_tag="OO572_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6661323.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            18844..20604
                     /gene="pbgA"
                     /locus_tag="OO572_002344"
     CDS             18844..20604
                     /gene="pbgA"
                     /locus_tag="OO572_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6661324.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20679..20755
                     /locus_tag="OO572_002345"
     tRNA            20679..20755
                     /locus_tag="OO572_002345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20713..20715,aa:Pro,seq:ggg)
     gene            complement(20858..>22433)
                     /gene="yejO"
                     /locus_tag="OO572_002346"
     CDS             complement(20858..>22433)
                     /gene="yejO"
                     /locus_tag="OO572_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6661325.1"
                     /translation="NMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVG
                     KVVVSEGASFRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVI
                     MMAEPVTRSSVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKI
                     FVTDTGASPAAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAEN
                     RAQITPSTTDVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTT
                     DAGAGFEQTLTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGA
                     YAGWEHQNGAYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGL
                     WSVRPYLAFTGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPW
                     LKAAVRQEYADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGA
                     GVESPWNTQAGVVWTF"
BASE COUNT         5493 a         5763 c         5534 g         5643 t
ORIGIN      
        1 tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc gtttttctga tgtccgtacc
       61 gggctgaaag tagaagagcg tttcaaaggt gatgatatcg ttgacaccgt gacgctgacc
      121 cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat atgtctttat ggataaagaa
      181 gactataccc cgtatacctt caccaaagat cagattgaag aagagttgct gtttatgcca
      241 gagggcggca tgccggacat gcaggtgctg acctgggacg gtcaactgct ggcgcttgag
      301 ctgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac caggcatcaa aggggcatct
      361 gccagcgccc gtaacaaacc ggcgacattg agcactggtc tggtgattca ggtaccagaa
      421 tacttaagcc cgggcgaaaa aattcgtatc catatcgaag aacgccgtta tatggggcgt
      481 gctgactaac ttcagccgca tgcagaaaag ggatagctca ggctgtccct tttttaattt
      541 attataccaa tcttctattt tgcgcttcct gaacacctta cgccctcaat tttcactcgt
      601 tgattgatcg ccctcacact tcatcgcatt aacaatccag accaacttca attgctgtca
      661 tataacttta cactgtcatt gttaatttat cgttactaag acgtgactcc tatgaagaca
      721 attgcctccg ttacgctccc gcatcatgta cacgctccac gctacgatcg ccagcagttg
      781 caatcacgta tcgttcattt tggctttggc gcctttcacc gcgctcatca ggcgttactg
      841 accgatcgtg tgctgaatgc ccagggcggc gactggggga tctgtgaaat cagcttgttc
      901 agcggtgatc aactgatgag ccagctccgc gcacagaacc atttatatac cgtgctggag
      961 aaaggtgcgg acggcaatca ggtgataatt gtcggtgccg ttcacgaatg ccttaatgca
     1021 aaactggatt ccttagcggc aattattgag aaattttgcg agccacaggt ggcaattgtt
     1081 tccctgacga ttaccgaaaa aggctattgt attgacccgg ccaccggtgc actcgacacc
     1141 agtaatccgc ggattattca cgatctacaa acccctgaag aacctcactc cgcaccgggt
     1201 attctcgtcg aagcactgaa acgccgccgt gagcgcagcc ttacaccgtt taccgtgctc
     1261 tcctgcgaca atattcccga caatggccat gtggtgaaaa acgcggtgct gagaatggca
     1321 gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac acgtcagttt tccgggaacc
     1381 atggtcgacc gcattgttcc ggctgcaacc gacgaatcac tggcagaaat cagccagcat
     1441 ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt ttatccagtg ggtggttgaa
     1501 gataacttca tcgctgggcg tcctgcctgg gaagtcgcag gtgtacaaat ggtgaatgat
     1561 gtcctgccat gggaagagat gaaactgcgt atgcttaatg gcagccactc ttttctcgct
     1621 tatctgggtt acctctcagg attcgcctat atcagtgatt gcatgcagga tcgcgtattt
     1681 cgctatgctg ccagaacatt aatgctggat gagcaagcgc cgacactgca aattaaagat
     1741 gtcgatttaa cacaatatgc ggataagtta attgcacgtt ttgctaatcc gtcgctgaaa
     1801 cataagacct ggcaaatcgc gatggatggc agccagaaat taccgcaacg tatgctggca
     1861 ggtattcgca tacatctggg gcgtgaaacg gactggtcgt tgctggcatt aggcgttgct
     1921 ggctggatgc gttacgtcag cggcgttgat gatgccggaa atgccattga tgttcgcgat
     1981 ccgcttagcg ataaaattcg cgaacttgtt gcgggcagca gcagtgaaca acgcgtaacc
     2041 gccctgcttt ccctgcgtga agttttcggt gatgatctgc cagataaccc gcattttgtg
     2101 caggccatcg aacaagcctg gcaacaaatc gtacaattcg gcgcacatca ggcgctatta
     2161 aacaccctca aaatttaacg atttctgcgg ttaaagcgga tgaagctcac cttcgtccgc
     2221 tctccccttc tcttttctgc cttttttagc caggattaac gctcagttaa cttaccagag
     2281 tattgcggag ccgttgtgac caggaccaac ctcatcaccg gttttctcgg cagcgggaaa
     2341 accacgtcga ttcttcatct gttagcccat aaagatccca acgaaaaatg ggcggtactg
     2401 gttaatgaat ttggggaagt cggaattgat ggtgctttgc tcgccgatag cggcgcattg
     2461 ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg gtttacccat gcaggtaggg
     2521 ttgaatacct tactgcgtca gggaaaacca gaccgcttgt tgatagagcc gaccgggctg
     2581 ggccatccga aacagatcct cgatctgtta accgcaccag tctatgaacc gtggatagat
     2641 ctgcgcgcca ccttgtgcat tctcgatcca cgcctgctgc tggacgaaaa aagcgccagc
     2701 aatgaaaact tccgtgacca gctggctgcc gcagacatca ttgtcgccaa taaatccgac
     2761 cgtgcgacgc ccgaaagtga gcaagcgcta cagcgttggt ggcagcaaaa tggtggcgat
     2821 cgacaattaa ttcacagtga gcatgggaaa gttgacggtc atcttctgga tttgccgcgt
     2881 cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc atcagcatgt cgtgaaaaaa
     2941 gggttagcag cgttaagcct gccagagcat caacgctggc gtcgcagtct gaacagcggg
     3001 caaggatatc aggcctgcgg ctggatattc gacgctgata cggtattcga caccattggc
     3061 attctggaat gggcgcgact tgcaccggtg gaacgcgtca aaggcgtgct gcgtattccc
     3121 gaagggctgg tacgaatcaa ccgtcagggc gatgacctgc acattgaaac gcaaaacgtt
     3181 gcgccaccgg acagccgtat tgagctgatt tccagcagcg aagctgactg gaatgccttg
     3241 cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt aaggttgcct gcgtttttca
     3301 gtaagataat tagagaaaat atgattaaaa atttgccgca aatagtgttg ttgaatattg
     3361 tcggccccgc gctgtttctt tcctggtata ttcccgttaa tcatggattc tggttgccga
     3421 ttgatgcgga tattttttat ttctttaatc agaaactggt cgaaagtaag gcctttttat
     3481 ggctggttgc attgactaac aatcgcgcct tcgacggttg ttcactgctg gcgatgggta
     3541 tgttgatgct gagtttctgg ctgaaagaaa acgcccctgg cagacgacgt atcgtgatta
     3601 tgggtctggt catgctatta actgcagtgg tattaaacca gctgggtcag gcattaattc
     3661 ctgtaaaacg ggccagccca acattgactt ttaccgatat taaccgcgtc agcgaactgc
     3721 tctctgttcc cacgaaagat gcctcacgag atagctttcc cggcgatcac ggcatgatgc
     3781 tgcttatttt ttcggcattc atgtggcgtt atttcggcaa agttgcaggc cttatcgccc
     3841 ttattatttt tatggttttt gcatttccca gagtaatgat tggcgcacac tggtttactg
     3901 acatcattgt cggttcgatg accgtgatat tgatcggttt gccctgggtg ttgctgacgc
     3961 cattaagtga tcgattaatc accttttttg acaaatcact accaggaaaa aacaaacatt
     4021 tccaaaacaa ataactcaca gtaattaaca tcatcagggt tatttttata gtgaggataa
     4081 tcctgatgat gcgcaccgtg ctttcatcta tcgaacgcaa aaatcattct ctaagtaaat
     4141 gaatggattg catgcgtttc actcaattgt actttaattg accaaccccg cttattaact
     4201 ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga ccgcaccaat
     4261 cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca tataattttg
     4321 tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg taattagtct
     4381 cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa
     4441 acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatt cccgcgattg
     4501 cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg
     4561 agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa
     4621 acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag
     4681 gcgtacgtta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac
     4741 agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttac gaacagcagg
     4801 aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt ctgttccgtg
     4861 ccggttcaac gggacgccat gtcggtattt atatcggcaa caatcagttt gtccatgctt
     4921 ccaccagcag tggtgttatt atttccagca tgaatgaacc gtactggaag aagcgttaca
     4981 acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct
     5041 atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt cattcatcag
     5101 agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa
     5161 tgttgtcgta atttcaggaa attaacggaa tcatgttcat acgcgctccc aattctggac
     5221 gtaagctcct gcttacctgc attgtagcag gcgtgatgat tgcgatactg gttagctgcc
     5281 ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg attatcgacg
     5341 tacaaaagta tctcgatacc tattttgccg acctgaaatc cactactgac cggcttcagc
     5401 cgctgacctt agatacctgc cagcaggcta accccgaact gaccgctcgc gcggcgttta
     5461 gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa aacattctgt tcatctgcga
     5521 ctggtgagat ggacattcca ctcaatgaat tgattccggc gctcgacatt aataaaaacg
     5581 tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct
     5641 ggtatcgcaa ccctttgctg aaaaatagcg gtgtgtttgc cgctctgaat ctcaacctga
     5701 cgccttctct cttttatagt tcacggcagg aagattacga tggcgtcgcc ctcattattg
     5761 gcaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa ttaaccgaca
     5821 tgccggtcca tgaaactaaa attgcgggca ttcctctgac cgttcggctt tatgcggatg
     5881 actggacatg gaacgatgtg tggtacgcat ttttactggg cggcatgagt ggaactgtcg
     5941 ttggcctgct ttgctattac ctgatgagcg tacgtatgcg ccccgacaga gaaatcatga
     6001 ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc ggtggtggat acacaagctt
     6061 tgcgggtaac gggcctggaa gtactgctac gctggcggca tcctgtagcg ggagaaattc
     6121 ccccggatgc cttcattaac tttgccgaat cgcaaaagat gattgtgccg ctgactcagc
     6181 acctgtttga gttaattgcc cgcgatgccg cagaattaga aaaagtactg ccggtaggcg
     6241 tcaaatttgg cattaacatt gcgccggacc atctgcacag cgaaagcttt aaagcagata
     6301 tccagaaact gctcacttcc ctgcccgcac accatttcca gattgtgctg gaaattaccg
     6361 agcgcgatat gctgaaagag caagaagcca cacaactctt cgcctggctg cactcggtcg
     6421 gcgtagaaat tgctattgat gacttcggca ccgggcacag cgcgcttatc tatcttgagc
     6481 gttttacgct cgattatctg aaaattgacc gtggatttat caacgccatc ggtacggaaa
     6541 cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgtctc aatatgctga
     6601 cagttactga aggggtcgaa acgccggaac aagcgcgatg gctaagcgaa cgcggcgtta
     6661 atttcatgca aggctactgg attagtcacc cgttaccgct ggacgatttt gttcgctggc
     6721 tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt attcatagtg
     6781 aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc
     6841 gcatactgct gctgtttatc gctctgttca ccttcggtgc gcaggcgcag gctatcaagg
     6901 aaagctatac ctttgccgtg ctgggcgaac cccggtacgc gtttaatttc aaccattttg
     6961 attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac gttgtcagcc ctcggtacct
     7021 tcgataattt caaccgctat gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt
     7081 acgacacgtt atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg
     7141 aaagtgcacg ctatgctgac gattattcct gggtggaggt cgctattaat ccgcgcgccc
     7201 gttttcatga tggttctccc attactgccc gcgatgtaga gtttactttt caaaaatttc
     7261 tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg
     7321 cgccgttaac cgtgcgcatt gagttagcta aacccggcaa agaagatatg ttgagtctgt
     7381 tttcgctgcc ggtatttcca gaaaagtact ggaaggatca caaacttagc gacccgctcg
     7441 ccacgcctcc gcttgccagt ggtccgtacc acgttacgtc ctggaaaatg gggcaaaata
     7501 ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgtggacgct
     7561 ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt
     7621 ttaaagcagg tgcctttgat ttgcgtatgg aaaacgacgc taaaaactgg gccacgcgtt
     7681 ataccggtaa aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag
     7741 cccaggatac gcgctggctg gcgtttaata tccaacgtgc ggtattcagc gatcgccggg
     7801 tgcgggaagc aatcactctc gcctttgact ttgaatggat gaacaaggcg ttgttttaca
     7861 acgcctggag tcgcacgaac agttattttc aaaataccga atacgcggcc agaaattacc
     7921 ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg ccagaagtct
     7981 tcacacaaat ctaccagccg ccggtatcta aaggcgatgg ctacgatcgt gacaacctgt
     8041 taaaagccga caaacttctc aacgaagcgg gctgggtgct gaagggtcag caacgcgtta
     8101 atgccacaac gggtcagccg ctcagctttg aattattgct tcccgcaagc agcaatagtc
     8161 agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaatatg gacattcgca
     8221 aggtggataa ctcgcaaatc actaaccgca tgcgcaatcg cgactatgac atgatgccgc
     8281 gactatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat
     8341 atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac tcactgatca
     8401 accaaattat tgccgcgcag ggaaataaag aaaaattact gccgttgggg cgagcactgg
     8461 atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc
     8521 tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg
     8581 atacctggtg gtatgacgtt aataaagcgg ccaaactgcc gtcagccaga caacagggag
     8641 agtagatggg cgcttacctg attcgccgtc tgttgctggt gatcccaacg ttatgggcta
     8701 ttatcactat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gatcaggcca
     8761 tcgccgccat tgagtttggt aatgccggag tattacccgg cgcaggcggt gaaggtgttc
     8821 gtgccagcca tgcgcaaacg ggtgtcggca atatcagcga cagtaattac cgtggcggac
     8881 gcggattaga tccagaagtg atcgctgaga tcactcatcg ctacggtttc gataagccga
     8941 tccacgaacg ttacttcaaa atgctctggg actacatccg ctttgatttt ggtgatagcc
     9001 tgtttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtt tccatcaccc
     9061 tcggattgtg gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag
     9121 ctgtttataa tgggagtcgc tttgacgtct ggagtagcgc atttatcatc atcggctacg
     9181 ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcgggc ggcagctatt
     9241 tcgacctgtt ccctctacgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc
     9301 agaaaatcac cgattatctg tggcatatca cgctaccggt gctggcgaca gtgattggtg
     9361 gctttgcggc gctgaccatg ctgaccaaaa actcattcct tgatgaagtg cgcaagcaat
     9421 acgtggtgac cgcgcgtgcg aaaggggtaa gtgagaaaaa cattctctgg aaacatatgt
     9481 tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt
     9541 ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc ttactgggct
     9601 acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc
     9661 tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtt gatccgcgta
     9721 tagattttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc gttgggcgcg
     9781 ttttcgtcat aaccgtcgcg gctactggtc gttatggatt ttcctcgttt tgtttggttt
     9841 gagtttgtgt tctgaactta tcgctaacga taaaccgttg ctggtgcgtt atgacggcag
     9901 ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc cgctggcaag
     9961 tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataacg gctgggtact
    10021 gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc
    10081 ttctccaccc tcccggcaaa actggctggg aacggatgca aacggcggcg atgtgctggc
    10141 acgcattctc tatggcacgc ggatctcggt tctgtttggc ctgatgctga ctctctgttc
    10201 cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggcggta aagtcgatct
    10261 ctgggggcaa cgctttattg aagtatggtc ggggatgccg acgctgtttt tgattatttt
    10321 actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattactg tcttgtttgg
    10381 ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtactcgta atttcgacta
    10441 cattcgtgcg gcacaggcgc ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt
    10501 gcctaatgcc atggtcgcga ccctcacctt tttaccgttt attttatgta gttcgataac
    10561 caccctgacc tcgctcgatt tcctcggctt cggtctaccg ctcggttcac cgtcactcgg
    10621 tgaactgctg ttacaaggga aaaataacct tcaggccccg tggcttggga tcaccgcctt
    10681 cttgtcggtg gcgatattat tgtctttgct gatctttatt ggtgaagccg tccgcgacgc
    10741 atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaaaatttgt
    10801 cggtgggttt tcgccatcag caaaccgtac gtacagtagt caatgatgtt tcactacaga
    10861 ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa agcgttaccg
    10921 cgctgtcaat tttacgcctg ctcccttccc cgccggttga atatctctcc ggcgatattc
    10981 gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt gtacgcggta
    11041 ataagatcgc catgattttt caggaaccga tggtgtcgtt aaatccattg cataccctgg
    11101 aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg
    11161 gcgaaattct taactgcctt gatcgcgtcg gtatccgcca ggcggcaaaa cggctgacag
    11221 attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt
    11281 taacgcgacc ggaattatta attgccgatg aaccgaccac cgcactggac gtctctgtcc
    11341 aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg ggcatgctgt
    11401 ttattactca taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa
    11461 acggtcgctg tgtcgagcaa aataacgccg ctacgctatt tgcctcaccc actcatcctt
    11521 acacacaaaa gctactcaac agtgaaccgt caggcgatcc ggtaccgttg ccagaacctg
    11581 cctcaacgtt gctggatgtt gaacagcttc aggttgcctt ccccattcgc aaagggattt
    11641 tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgggcgg
    11701 gtgaaacact gggtttagtg ggcgagtctg gttccgggaa aagcacgacg ggactggcgc
    11761 tgctgcgact gattacctct cagggcagca tcgtctttga cggtcagcca ctgcaagatt
    11821 taaatcgccg ccagctgtta cctactcgtc atcgtattca ggtggtattt caggatccaa
    11881 actcctcgct caacccacga ctcaacgttt tgcagattat tgaggaaggc ttacgggttc
    11941 accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg atgcatgaag
    12001 tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcgac
    12061 aacgtattgc gattgccagg gcattaattc ttaagccctc gctgatcata cttgatgaac
    12121 cgacatcatc actcgacaaa acggtacagg cgcaaatatt gacgcttttg aaatcattgc
    12181 aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt gtccgcgcgt
    12241 tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc
    12301 gcgtgtttgc cacaccgcag caggagtata cgcgtcagtt actggcgttg agctgacgct
    12361 taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg
    12421 catgttgcag caaactcgtc ctggcgattt acaaacaggc acggctcacc ttcacactcc
    12481 aacaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttata catcaccgac
    12541 catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac
    12601 ggtccatttt gaggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag
    12661 ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag
    12721 tacattcact tcacaatttg tttacatcaa ttttaacaca tcatcaacaa tccgtcttta
    12781 tacagatcgt aaagattgat gcctcgcctg cgtggcctct atggctctga tttaagtaat
    12841 atctgcgggc gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tctaactcaa
    12901 cctttttaac tacaatgatg tgacttttta cataaattga ttttacataa aataaacata
    12961 tatcggggaa atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttaccttc
    13021 ctcgttgtgc aatagatcac cgttttttcg gccgactggc gtacagacag aagagaatgg
    13081 agctggttgc gcagaatgca attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa
    13141 gagaaagcaa tgcgccaaca attgccccta tgccaaaacg gaaggttcct gccagcgaag
    13201 atgccgttcc cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca
    13261 ccatcgacac gcagcccaca aacgccgcaa cgccaaccac cagcgaccag aatcccagcc
    13321 ccagcggcgc actgatgacc atccacgcag ccataataaa ttgtatccac aaccccgagc
    13381 ggaacatatt taacgcgcca atgcggcgga cgaagcggct gttaaagatg gtcatcacga
    13441 acagaaaaac aatgtttagc gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa
    13501 tttcaatata aacaaacggt ccggcgctta agaatgagaa catcccggca aagctgaaac
    13561 cactggcaag catgtagctc aggacacgtt tatggcggaa cagcgccgca aagttaccaa
    13621 tagtggtacg aatgtgaaat ggctgacgac gctccggtgg taaggtttct ttaatcagga
    13681 agaaaatcat tgccgaagcc agaatcgccg ctaatgccag gatccagaag atgtaatgcc
    13741 agctcagcca caccagcacc cagccgccaa ctatcggtgc catcagcagt gcaatggtcg
    13801 tcaccagcat gacaaacgac atcatccgcg agaactcttc tttcgggtaa atatcacgca
    13861 tcaaagcgtt gataaccacg ctggccgcag ccgcagccag cccgtggaag aaacgcatca
    13921 caatcagctg atcgatggtt tgtgccaacg cacacgccac cgcggcggcg gcaaacacca
    13981 gcgtcccgcc aagaaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta
    14041 actgccccaa cgcaaagccc agaatataag tactgagggt catctgcgta ctgcccgccg
    14101 atacgccaaa ctgcgctgaa attaccggta gcgcgggcag atacatatca atcgacagcg
    14161 gcatcaacat ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg
    14221 tggtcacaac gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt
    14281 cagttaacgg acgatattca ccgggggcta aatcagcatc cagcgtaata ccgccaatac
    14341 gttcacgatg cagctcaacc acgtggttac ccacggcggc gaacatgcgt ttcacctgat
    14401 gataacgccc ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tcaagcaccg
    14461 ccggcttagt gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg
    14521 tatcgtcagc tacaggtgat tccagtgtca ccagataggt cttctcgcaa tgatggcgcg
    14581 gagaagtaat gcggtgcgac cactgaccat catcagtcat cagcaccaga ccggtggtat
    14641 cgatatccag acgtcctgcg gcgtgcagtt tccacgctac cggttcatcc agaaaataga
    14701 gcaccgtcgg gtgatccggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca
    14761 tgaagtaacg tggaccgtgt tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt
    14821 cagggagcag tttgaacgct gcattacgaa cgatttcgcc atcgacggtg acacgattgc
    14881 cgcggatttc acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat
    14941 caagtcgcat gtgtgtgatt ttgcctgtaa aaaacggagg tcgggtaatg acccgaaaat
    15001 ctgaactgtt gtctgcccag tatagcggtc taataacttc cctcaaggga aaaagaaccg
    15061 tggcatacta ttagcagaat aatctaccta cgcgaaacca tgatttttac actccgccca
    15121 tatcagcaag aagccgtgga tgccacgctc aaccattttc gtcgtcataa aacccctgcc
    15181 gttatcgtgc tgcccaccgg cgcaggtaaa agcctggtga tagcggaact ggcacggctg
    15241 gcgcgtggtc gcgtactggt gctggcacac gttaaagaac tggtggcgca aaaccatgca
    15301 aagtatcagg cgctggggct ggaagccgat atttttgccg ccgggctaaa gcgcaaagag
    15361 agccacggta aagtggtatt tggcagcgtt cagtcggtcg cccgtaatct tgatgccttt
    15421 cagggtgaat tttcgctgtt gattgtcgat gaatgccacc gtattggtga cgatgaagag
    15481 agccagtatc agcaaatcct cactcacctg actaaagtga atccccactt acgcctgctg
    15541 gggctgactg ccacgccttt tcgattgggc aaaggctgga tctaccagtt ccattatcac
    15601 ggcatggtac gcggcgatga gaaagccctt ttccgtgact gcatttatga gctgccgctg
    15661 cgttatatga ttaaacacgg ctatctgacg ccgccagaac gactggatat gccagtagtg
    15721 caatacgatt tcagccgctt gcaggcacag agtaacgggc tgttcagcga agctgatctc
    15781 aaccgtgagc tgaaaaaaca gcaacgcatt acgccacata tcatcagcca gatcatggag
    15841 ttcgccgaaa agcgcaaagg ggtaatgatt ttcgccgcca ccgttgaaca cgcaaaagag
    15901 attgtgggat tactgcctgc cgaagatgct gcactgatta ctggcgacaa ccccggcgct
    15961 gagcgcgatg tgttaattga agattttaaa gcccagcgtt ttcgctatct ggtcaacgtc
    16021 gcagtactga ccaccggatt tgacgccccg cacgtcgatc ttatcgccat tctgcgccct
    16081 accgaatcag tgagtcttta ccaacaaatt gtcgggcgcg gtctgcgtct cgctccgggc
    16141 aagactgatt gtttaattct tgattatgcg ggtaatcctc acgatctcta cgcgccggaa
    16201 gttggtacac cgaaaggcaa aagtgacaac gttccggtac aggttttctg ccctgcctgc
    16261 ggttttgcca acaccttttg ggggaaaacg accgccgacg ggacattgat tgaacacttt
    16321 ggtcgccgct gtcagggatg gtttgaagat gacgacggtc atcgcgaaca gtgtgacttc
    16381 cgtttccgtt ttaaaaattg cccgcaatgt aacgcagaaa atgatattgc cgctcgccgc
    16441 tgccgcgaat gtgacacggt actggttgat ccggatgata tgttaaaagc ggcgctacga
    16501 ctgaaagatg cgctggtatt acgctgtagc ggcatgtctt tgcagcatgg gcacgacgag
    16561 aaaggcgaat ggttgaaaat cacctattac gatgaagacg gcgcggatgt gagtgagcgt
    16621 ttccgtctgc aaacgcccgc ccagcgtacc gccttcgagc agctttttat ccgcccgcat
    16681 acgcgcacac cgggcatccc tctgcgctgg atcaccgccg ccgatatcct cgcccagcaa
    16741 gccttactgc gacatccgga ttttgtcgtc gcccgcatga aaggtcagta ctggcaagtg
    16801 cgtgaaaaag tgttcgatta cgaaggtcgt tttcgtcggg cgcacgaatt acgcggttaa
    16861 tggtagtttt gattgatgta taaggcgatt gagtatagaa tctcgcccgc ttttgcatac
    16921 gcaaagcaga tcacttacct gttgctgggt cgcctgtagc aggattaatt taagagagaa
    16981 agaaatgttt actatcaacg cagaagtacg taaagagcag ggtaagggtg cgagccgccg
    17041 cctgcgtgcc gctaacaagt tcccggcaat catctacggt ggcaaagaag caccgctggc
    17101 tatcgagctg gatcacgaca aagtcatgaa catgcaagct aaagctgaat tctacagcga
    17161 agttctaacc atcgttgttg acggtaaaga aatcaaagtt aaagctcagg acgtacagcg
    17221 tcacccgtac aaaccgaagc tgcagcacat cgacttcgtt cgcgcttaat tgctgaataa
    17281 gttgtaaaaa accccgctcc ggcggggttt tttgtatctg cagattatgc ctgattacgg
    17341 tattgctatt ttttgcaggc cagataaggc gttcacgccg catctggcat tcagaacaca
    17401 aaaccgatta attgccgcca gacgtccggc gctgcaattg gtcgcgcaaa ttcggcggcg
    17461 tgcctttaat ggtcaaggta tcggttgctg gatcccagaa gatgcgttca cctaacagca
    17521 tcgcatcaaa gttaatagtc aagccaccgc cgctaccggc aaacttcgtc aactgacgca
    17581 gtgtgctacg gtctgccggg aagctctctt ccagttcgta gcctttttca gccgcaaact
    17641 ccgtgaagct cacttcgctt acgcctgcca gctctttcga cagcgattcc agttcgatct
    17701 cttcgcctgc ctgcagctgc tcattacagt agctataaac ctgctggcgc acgttctgtc
    17761 gctctgcttt atccagttgt gcttcggcag tgaaatcatc aaccgcttgc aacagcccac
    17821 ggttttgcgc tttcgcgttc aatccttcgc tggccccgag gaaatccata aagaaatccg
    17881 ccactttgcg ccctactcgc cctttcagga aagtgagata gcgggtggac tctggattgg
    17941 tttcccattc ggttaaatcg atacgcgcaa caatatccgc atgattgata tcaagataat
    18001 gggttgggtt gatgtcgaga ttttcgttaa cgcgcatact gctcaggttg ctcagaactg
    18061 ccaccagcaa atactccacc gccagataac ggtagtggca aaacagcacg aatccaccgt
    18121 cagcaaaagg atatttcgct aattcgtcac gcaggcgacc ggttgccgcg cggctaaatg
    18181 ccagaaagtc ctcttcgccc tgacgctgta agcgcagcgt ctgcgccagt tcactctctt
    18241 cgctaaacaa gccgtaagct ttatttttgg cgctatagac ccgatgcagt tctgccacca
    18301 tctcaacgac ggtttctgtc ggttccagca atgaatcgcg caacaccagc tcaaggttct
    18361 gctcatcacg cttgataagc tggtgcagag caatctggtt gatatccaga ctcatgataa
    18421 actctccttt aagaccgggc ggtattcaac caccgccagt taagcgacgc aataaaagat
    18481 ggcggtcatt ttacagaagg cagcgtaacg agaatgattt aacgcataaa aaagcagaaa
    18541 aaaaaccgtt gctacggtaa tatgttgccc tttcatcaac taactgattt cgatttatgc
    18601 cacaaatttc ccgctacagt aatgaacagg ttgaacaatt gctcgcagag ctgctcaacg
    18661 tactggaaaa acataaggct ccgaccgacc tttccctgat ggtgttaggc aacatggtca
    18721 ctaaccttat caacaccagc attgccccgg cccaacgcca ggcaattgcc aactcttttg
    18781 ccagcgcctt acagtcctct atcaacgaag acaaagcgca ctaagggaaa cagataacag
    18841 gttatggtaa ctcatcgtca gcgctaccgt gaaaaagtct cccagatggt cagttggggg
    18901 cactggtttg cactgttcaa tattctgctt tcgctcgtca ttggcagccg ttacctgttt
    18961 atcgccgact ggccgacaac gcttgctggt cgcatttatt cctacgtaag cattatcggc
    19021 catttcagct tcctagtgtt cgccacctac ttgctgatcc tcttcccgct gacctttatc
    19081 gtcggctccc agaggctgat gaggtttttg tccgtcattc tggcgacggc gggaatgacg
    19141 ctattactga tcgatagcga agtctttact cgtttccatc tccatcttaa tcccatcgtc
    19201 tggcagttgg ttatcaaccc agacgaaaat gagatggcgc gcgactggca gctgatgttc
    19261 atcagcgtgc cggttatttt attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag
    19321 ctgcgcagcc tgacgcgtcg tcgacgcttc gcgcgcccgc tggccgcatt cttatttatc
    19381 gcctttatcg cctcgcatgt ggtgtatatc tgggccgatg ccaacttcta tcgcccgatc
    19441 accatgcagc gcgctaacct gccgctttcg tacccgatga cggcgcgacg tttccttgag
    19501 aagcatggcc tgcttgatgc gcaggagtat caacgccgtc ttattgagca aggtaatcca
    19561 gacgccgttt ccgtacagta tccgttaagc gaactgcgct atcgcgatat gggcaccggg
    19621 cagaatgtgc tgttgattac tgtcgatggc ctgaactact cacgcttcga gaagcagatg
    19681 cctgcgctgg caggttttgc tgagcaaaat atttcgttca cgcgccatat gagctccggc
    19741 aacactacag acaacggcat ctttggcctg ttctatggca tctcgccgag ctatatggac
    19801 ggcattctgt cgacccgtac gcctgcggca ttaattactg cgcttaatca gcaaggctat
    19861 cagctggggt tattctcatc agatggcttt accagcccgc tgtatcgcca ggcattgttg
    19921 tcagatttct cgatgccgag cgtacgcacc caatctgacg atcagaccgc cacgcagtgg
    19981 atcaactggc tgggccgcta cgcacaagaa gataaccgct ggttctcgtg ggtttctttc
    20041 aatggcacta acattgacga cagcaatcag caggcatttg cacggaaata tagccgggcg
    20101 gcaggcaatg tcgatgacca gatcaaacgc gtgctcaatg cactgcgtga ttctggcaaa
    20161 ctggacaata cggtggtgat tatcactgcc ggtcggggta ttccactgag cgaagaggaa
    20221 gaaacctttg actggtccca cggtcatctg caggtgccat tagtgattca ctggccaggc
    20281 acgccggcgc agcgtattaa tgctctgact gatcataccg atctgatgac gacgctgatg
    20341 caacgcctgc tgcatgtcag cacacctgcc agcgaatatt cgcaaggtca ggatttgttc
    20401 aaccctcaac gccgtcatta ctgggttact gctgcggata acgatacgct ggcaattacc
    20461 accccgaaaa agacgctggt gctgaacaat aacggtaaat accgcactta caacttacgt
    20521 ggtgaaagag tgaaagatga aaaaccacag ttaagtttgt tattgcaagt gctgacagac
    20581 gagaagcgtt ttatcgctaa ctgattaatt ataaatcagt tagcgaaata tcttacttgc
    20641 aatcggtgtg gaaaacggta gtattagcag ccacgagtcg gcacgtagcg cagcctggta
    20701 gcgcaccgtc atggggtgtc gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat
    20761 cccaagaaaa aaccaaccct tacggttggt ttttttatat ctgcaattaa ttcgataaac
    20821 agaccgtgac acatcacagc ctgtttattt tctgttatca gaacgtccag accacacccg
    20881 cctgagtatt ccacggcgat tctacccctg cgccattgcc atagctgact gacaaatgac
    20941 cgcttaacgt cggggtaaac gatgacctta tcccagcctg ataaacgcca ctggttccag
    21001 ccacatcatt attaaatttg ccatcgtcat taactttcac ctggttagaa tcggcgtatt
    21061 cctgacgcac ggcggctttc agccagggtt ccagcgtcgt accgttttgc aggtccatgt
    21121 gatagcttac cgccgttccc gcttcagcgc gtaatatccg ggtatttccc acatccgcgc
    21181 gcatgccgtt tgataacgtg tagtcctgac catctgtggt aaagccggta aaggccagat
    21241 agggtctaac actccacaat ccgtcaaccc aacggaaccc gctctcaaca tgagcacccg
    21301 cgccgttact attgtaatcg ccaaacgctg ttgccccatt actcatcttg ccatggatgg
    21361 tgttggcaaa acggtcaact ttcaccaccc catcaacata ggcaccgttc tgatgctccc
    21421 aaccggcata agcccccagg gtatagctat cgatattacc ttttccgccg cgatcaaaac
    21481 caatatcaga atgagagtaa ccaaagaaca agccgcgaat tgtactgctt tcttcacggg
    21541 agaaacggct atcgataccg agcgtcaggc ccgtcaatgt ttgctcaaaa ccagctcccg
    21601 catcagtggt cacgttgttg cgggtgttaa ttgccgaact ccacatcacc gtatcgtaac
    21661 taacgccttt tacgctacca agacgctcac gcacggtgtc cagttctgca tcaaatacca
    21721 gcggttgtgc ggctgccatg ttcagcacat cagtggttga aggggtaatt tgcgcgcgat
    21781 tctctgcgag gctccagcta tggttgccat tatccagcaa ggtatattca tacgtaccga
    21841 tatcaacaac gcctccggca ttgcccaacg taaatgcagc atcaccgccg cccgttgtta
    21901 ccagtgtaag gctatctcct gctgccgggc tggcaccggt gtccgtcacg aatattttga
    21961 aatcacctgt tgcctgaccg gtgacgttga gctgatcgct ctgatgattg gccatatcgg
    22021 tacgcatata aaaattgccg tttcccgaca gggtattggt ggtcaacgta atgaaatttt
    22081 ccgcacttgc cgtcacagat gaacgagtca ctggctcagc catcataatc acatttgcgc
    22141 cggacatcgc aaggttggct aagttgaggc gagtgttttg gttcgtcgta gtgatatcgg
    22201 caatgatggt ccatgcgcta ttttcgagcg aaacgtccgc tttgctggta tccacggcac
    22261 catgcgttct aaaagaagcg ccctcagaaa ccactacctt acccaccaac atagcgggtg
    22321 cataatttat ttgcggcttc atgacctcaa gaatgccatc attccctctg actgacacgt
    22381 taaccattgt gccagcccag acattagcgc ggccattgtt gattaccata ttt
//
LOCUS       DAJTYL010000062        22168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYL010000062 DAJTYL010000000
VERSION     DAJTYL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.062"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            83..2683
                     /locus_tag="OO572_002347"
     CDS             83..2683
                     /locus_tag="OO572_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6661326.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2674..3657
                     /locus_tag="OO572_002348"
     CDS             2674..3657
                     /locus_tag="OO572_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6661327.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3755..4295
                     /locus_tag="OO572_002349"
                     /pseudo
     CDS             3755..4295
                     /locus_tag="OO572_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4303..4818
                     /locus_tag="OO572_002350"
     CDS             4303..4818
                     /locus_tag="OO572_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6661328.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4784..5521
                     /locus_tag="OO572_002351"
     CDS             4784..5521
                     /locus_tag="OO572_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6661329.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5632..6642
                     /gene="pyrD"
                     /locus_tag="OO572_002352"
     CDS             5632..6642
                     /gene="pyrD"
                     /locus_tag="OO572_002352"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6661330.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6816..7358
                     /gene="zapC"
                     /locus_tag="OO572_002353"
     CDS             6816..7358
                     /gene="zapC"
                     /locus_tag="OO572_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6661331.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO572_002354"
     CDS             complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO572_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6661332.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO572_002355"
     CDS             8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO572_002355"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6661333.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10828..12735
                     /locus_tag="OO572_002356"
     CDS             10828..12735
                     /locus_tag="OO572_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6661334.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12865..14118
                     /gene="pqiA"
                     /locus_tag="OO572_002357"
     CDS             12865..14118
                     /gene="pqiA"
                     /locus_tag="OO572_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6661335.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14123..15763
                     /gene="pqiB"
                     /locus_tag="OO572_002358"
     CDS             14123..15763
                     /gene="pqiB"
                     /locus_tag="OO572_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6661336.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15760..16323
                     /gene="pqiC"
                     /locus_tag="OO572_002359"
     CDS             15760..16323
                     /gene="pqiC"
                     /locus_tag="OO572_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6661337.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16579..16746
                     /gene="rmf"
                     /locus_tag="OO572_002360"
     CDS             16579..16746
                     /gene="rmf"
                     /locus_tag="OO572_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6661338.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO572_002361"
     CDS             complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO572_002361"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6661339.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17403..19163)
                     /locus_tag="OO572_002362"
     CDS             complement(17403..19163)
                     /locus_tag="OO572_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6661340.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19348..19800
                     /gene="matP"
                     /locus_tag="OO572_002363"
     CDS             19348..19800
                     /gene="matP"
                     /locus_tag="OO572_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6661341.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO572_002364"
     CDS             complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO572_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6661342.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO572_002365"
     CDS             complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO572_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6661343.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22006..>22168
                     /gene="sxy"
                     /locus_tag="OO572_002366"
     CDS             22006..>22168
                     /gene="sxy"
                     /locus_tag="OO572_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6661344.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACE"
BASE COUNT         5650 a         5206 c         5812 g         5500 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctatta ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag
      661 tgattctggc gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag
      721 attacgcaac aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct
      781 cagcagctat ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga
      841 ttatactgca ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga
      901 tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa
      961 aagcaatgcc cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc
     1021 gcctggtgct tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt
     1081 tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc
     1141 attactccag cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa
     1201 tagtaatgaa cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg
     1261 gggaacgaca tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg
     1321 tctgggattt aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag
     1381 tacgctggca gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac
     1441 gctcaaccaa ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt
     1501 ctacaccctt tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga
     1561 tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa
     1621 actgcaggtc aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag
     1681 ccagcaaact tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac
     1741 gcaaattaaa gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc
     1801 tgatgctgat caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag
     1861 aagtaatgat agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat
     1921 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     1981 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2041 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2101 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2161 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2221 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2281 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     2341 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     2401 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     2461 tataccgctg cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat
     2521 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     2581 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     2641 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     2701 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     2761 gcttatatcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     2821 gttaaaactc tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     2881 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     2941 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3001 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3061 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3121 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3181 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3241 tgctacaaca tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat
     3301 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     3361 gaggttgatc tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt
     3421 ggggccagaa cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta
     3481 gttaacgtca atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag
     3541 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct
     3601 gctggtggga cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg
     3661 tctatccagt cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     3721 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg
     3781 cgttattttg ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
     3841 cagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
     3901 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt
     3961 aagcactgct gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc
     4021 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
     4081 cacaggcgga agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc
     4141 agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca
     4201 cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga
     4261 cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc
     4321 tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg
     4381 gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa
     4441 atcaatgtgc agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg
     4501 gagaaatctt tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc
     4561 accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg
     4621 gatgcagcca gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt
     4681 aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg
     4741 caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt
     4801 gtacttcatt atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc
     4861 gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt
     4921 tccggcagac agagaatcga tatctatttt actgactaat accagtcagg aatcttggct
     4981 tattaacagc aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc
     5041 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     5101 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     5161 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     5221 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     5281 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5341 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5401 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc
     5461 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5521 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5581 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5641 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5701 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5761 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5821 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5881 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5941 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     6001 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     6061 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     6121 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     6181 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     6241 ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct
     6301 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6361 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6421 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6481 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6541 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6601 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6661 ctttataaga taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg
     6721 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6781 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6841 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6901 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6961 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     7021 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     7081 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     7141 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     7201 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     7261 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7321 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7381 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7441 gtgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7501 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7561 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7621 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7681 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7741 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7801 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7861 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7921 ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac
     7981 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     8041 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     8101 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     8161 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     8221 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     8281 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8341 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8401 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8461 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8521 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8581 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8641 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8701 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8761 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8821 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8881 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8941 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     9001 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     9061 tgctttcaca cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     9121 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     9181 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     9241 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9301 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9361 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9421 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt
     9481 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9541 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9601 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9661 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9721 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9781 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9841 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9901 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9961 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
    10021 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
    10081 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
    10141 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
    10201 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
    10261 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10321 aggcaaagat ttcctcaacc tgttttctta taccggcagc gccaccgtgc atgcgggatt
    10381 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10441 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10501 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10561 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10621 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10681 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10741 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10801 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10861 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10921 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10981 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    11041 caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    11101 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    11161 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    11221 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    11281 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11341 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11401 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11461 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11521 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11581 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11641 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11701 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11761 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11821 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11881 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11941 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    12001 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    12061 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    12121 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    12181 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    12241 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12301 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12361 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12421 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12481 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12541 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12601 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12661 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12721 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12781 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12841 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12901 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12961 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    13021 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    13081 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    13141 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    13201 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    13261 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13321 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13381 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13441 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13501 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13561 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13621 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13681 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13741 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13801 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13861 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13921 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13981 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    14041 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    14101 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    14161 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    14221 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    14281 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14341 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14401 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14461 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14521 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14581 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14641 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14701 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14761 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14821 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14881 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14941 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    15001 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    15061 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    15121 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    15181 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    15241 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15301 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15361 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15421 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15481 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15541 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15601 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15661 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15721 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15781 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15841 ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg
    15901 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15961 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    16021 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    16081 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    16141 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    16201 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    16261 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16321 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16381 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16441 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16501 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16561 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16621 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16681 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16741 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16801 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16861 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16921 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16981 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    17041 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    17101 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    17161 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    17221 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    17281 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17341 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17401 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17461 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17521 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17581 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17641 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17701 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17761 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17821 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17881 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17941 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    18001 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    18061 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    18121 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    18181 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    18241 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18301 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18361 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18421 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18481 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18541 cctaccagaa tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc
    18601 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18661 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18721 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18781 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18841 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18901 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18961 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    19021 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    19081 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    19141 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    19201 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    19261 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19321 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19381 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19441 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19501 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19561 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19621 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19681 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19741 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19801 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19861 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19921 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19981 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    20041 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    20101 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    20161 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    20221 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    20281 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20341 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20401 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20461 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20521 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20581 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20641 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20701 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20761 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20821 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20881 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20941 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    21001 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    21061 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    21121 agtcttgtat aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg
    21181 gaaaccgctt cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg
    21241 gcgacaaaaa aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa
    21301 tttttagccc ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa
    21361 accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
    21421 aatcatctgt caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
    21481 ttgactccac tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta
    21541 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
    21601 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
    21661 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
    21721 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
    21781 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac
    21841 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa
    21901 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg
    21961 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
    22021 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
    22081 tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat
    22141 ggtgagttgt atcttcgggc ttgtgagc
//
LOCUS       DAJTYL010000063        22136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYL010000063 DAJTYL010000000
VERSION     DAJTYL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.063"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..883)
                     /locus_tag="OO572_002367"
     CDS             complement(<1..883)
                     /locus_tag="OO572_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661345.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTF"
     gene            complement(985..1965)
                     /locus_tag="OO572_002368"
     CDS             complement(985..1965)
                     /locus_tag="OO572_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6661346.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1973..2683)
                     /locus_tag="OO572_002369"
     CDS             complement(1973..2683)
                     /locus_tag="OO572_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6661347.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2900..4723)
                     /gene="typA"
                     /locus_tag="OO572_002370"
     CDS             complement(2900..4723)
                     /gene="typA"
                     /locus_tag="OO572_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6661348.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5096..6505
                     /gene="glnA"
                     /locus_tag="OO572_002371"
     CDS             5096..6505
                     /gene="glnA"
                     /locus_tag="OO572_002371"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6661349.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6678..7727
                     /gene="glnL"
                     /locus_tag="OO572_002372"
     CDS             6678..7727
                     /gene="glnL"
                     /locus_tag="OO572_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6661350.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7739..9148
                     /gene="glnG"
                     /locus_tag="OO572_002373"
     CDS             7739..9148
                     /gene="glnG"
                     /locus_tag="OO572_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6661351.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9260..9370
                     /locus_tag="OO572_002374"
     CDS             9260..9370
                     /locus_tag="OO572_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6661352.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9599..10972)
                     /gene="hemN"
                     /locus_tag="OO572_002375"
     CDS             complement(9599..10972)
                     /gene="hemN"
                     /locus_tag="OO572_002375"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6661353.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11161..11670)
                     /gene="yihI"
                     /locus_tag="OO572_002376"
     CDS             complement(11161..11670)
                     /gene="yihI"
                     /locus_tag="OO572_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6661354.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12252..12884
                     /locus_tag="OO572_002377"
     CDS             12252..12884
                     /locus_tag="OO572_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6661355.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13266..16052)
                     /gene="polA"
                     /locus_tag="OO572_002378"
     CDS             complement(13266..16052)
                     /gene="polA"
                     /locus_tag="OO572_002378"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6661356.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16416..17348
                     /locus_tag="OO572_002379"
     CDS             16416..17348
                     /locus_tag="OO572_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6661357.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17389..18819)
                     /locus_tag="OO572_002380"
     CDS             complement(17389..18819)
                     /locus_tag="OO572_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6661358.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYSPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18974..19600)
                     /gene="dsbA"
                     /locus_tag="OO572_002381"
     CDS             complement(18974..19600)
                     /gene="dsbA"
                     /locus_tag="OO572_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6661359.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19617..20603)
                     /gene="srkA"
                     /locus_tag="OO572_002382"
     CDS             complement(19617..20603)
                     /gene="srkA"
                     /locus_tag="OO572_002382"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6661360.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20680..20949)
                     /locus_tag="OO572_002383"
     CDS             complement(20680..20949)
                     /locus_tag="OO572_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6661361.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21019..21603
                     /gene="mobA"
                     /locus_tag="OO572_002384"
     CDS             21019..21603
                     /gene="mobA"
                     /locus_tag="OO572_002384"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6661362.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21585..22112
                     /gene="mobB"
                     /locus_tag="OO572_002385"
     CDS             21585..22112
                     /gene="mobB"
                     /locus_tag="OO572_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6661363.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5473 a         5544 c         5299 g         5820 t
ORIGIN      
        1 taaaggtgta gcatagatat ttactcggcg atttcaggat cttatctgaa atcatgccac
       61 caatcggtcc accaatcatt ttcagacagt attggttaat gatgccgtaa gcccccacca
      121 gcgcaacggg caatagatag atgtttttca ggaatggaat gaagaatgtc aggccacagt
      181 aaaccgcgta aacgaagaag acgttgaaag cgataagcca gatggttttg tctctcagca
      241 ctgaggtcat actggtgttt tgcgatgctc cgtcttcttt tttcacctca acggacggtg
      301 cctcttcttt gtcattcagg acaaagaaaa taataatgcc tacggcaatc acaataaggg
      361 aatagaacca gatgcctgct ttaaaaccta ataagccact gccaaaccag gtaaatactg
      421 ccaacgcaga aaatgccacc acggtatcga caatgccacg ccctgtttcg aagaagccaa
      481 acaaccgacc ttgttgttcg ctattgccca atcgacttac cgacttgagc aagaccggcc
      541 agttcatcat gtcgcaagta acgccaaata gcgcccagac aaagaggatg ccccaatacc
      601 ccggcattgt cgtcaaataa acacccagta atcctgtcgc aatgagtgag aatgacatgg
      661 tgtatctgcg cggtagttta tcggcaaaat aaatagacag aaaaaagccc actgtggtga
      721 caaatgagtt taccgacata gcattaccaa tttgaccatt ggtcaaatgg aaatattcct
      781 gcatcgggat ataaaacgca tctttcagcg acggtaattt ataaattgta ccgccacaca
      841 gtgttaatag actaaataac gcccatttct ttttcgtgag cataacatcc cctaagcgat
      901 ttttaaaatc actgttttaa aggcaataag ataccgacca ttcatatgga atggttttat
      961 taactgcgta tgagttaaaa cttattagtg gttcagaaga tggtttgcct cttgtttgat
     1021 ttgttgtagt acgttacgca catgatttat ctgattaatt gcatcttctt tttctttacg
     1081 taaccgcgca tccagtaaat gattttctgg taggggtgtt tcactcatct ggcgataagg
     1141 gatccacgga ttatcatctt cgtcttcaaa gcggaacgcc gacgcatagt aatcaacctc
     1201 ttcttccagg ccatatgccg tcacctgcgg ctcatcatcg ccaaggcaga taaggtgcgt
     1261 taataacgct ttgaatggca tatcaccctg acctgaaata caggctttat gacccaggcc
     1321 accctgttct ttaacgatca aggcatcttt aatatggacc tgggtaatat gcggtgccat
     1381 cgtttttaag gcgtcgatgg gatgctcatt tgcattgatc atgttagcaa aatcaaacag
     1441 taaggaaaga gactccatct cactttcttt gaccagcgac accagctcat gactttttaa
     1501 atcttcatgc tgctcaatcg taaaagtcag gccgctgtcc tgatacgttt cccgtacata
     1561 ggcaatgtcg ttagcgataa tcgataatac gtcgcgcaaa ttaccttcat aacatggata
     1621 aaaacgtacg gacgttgccc cagttttcaa cgcgatggcg acggcttcgt caatagatgc
     1681 cttatctgaa gcgctggttt caatatgaat atcaaggttc agacggcggg ctttatcccc
     1741 aaaggcagag agttctttat cgtccatatt accaagagaa aaacgctcgc catccagaac
     1801 atggattttg acgccacgta gattattttc actggcgata tcaagtaaat cagcaggcaa
     1861 cactctttcc agccgcatat ttaaatgaaa ggcataagca tgtaaataaa gaggaagagt
     1921 gtcgacacgt tgtagaatct ttcttgcgtt atttattgtc accataaaac acctacagat
     1981 taatgatgag gtctacggcc tcaaatcgcc aatattcaat atctatctgg acaatattgc
     2041 cttgctggtc ggcgcgatgc ttttcaatta agatcgctgg catacccgca gaaccgccaa
     2101 gatacttgct gatatcgccc ggcattctga cgggtttgaa aatcaatttt tttactaccg
     2161 tctctttttg gtagcacttt tcccagactg cagaaaatga gtggttttca agttgttcaa
     2221 taaaacccgg agcaacttca ggattaatat atgtttcgtg atagaaaact ttatgtcctt
     2281 ccagcgcccc ccagccggtg atgcgataga gttcagttga tggcgtcact gcaatcagtg
     2341 gcgcgagcgt ctcgggaata tcgctggtac ggtttttctc ggtaaacccc caagaaggct
     2401 ctcgcccttg ctcaattgcg gcccgctgaa agctcgccga cagctccgga ctgtaattaa
     2461 atcgcggctg ggtcacgaac caccccttac gatccttacg gaagattttg gattctgcct
     2521 cgaggttcaa caacgcctgg cgcagcgtca tacgtttaat gcccagcagt tctcccagct
     2581 ctctttctga agggagtttt ccacccggcg tggtgatgcc atctttcaac caccgatcca
     2641 gtttctcttt tgcattttct acggtggatt gattctccgc cattgtttaa tccttttggt
     2701 ttaaaccaga tgaagcatct tacgcttacg ccaaaaaaga aagcaattaa cggtgtatga
     2761 ctttgcagat aattgtgaca aagctctcta ttgacgtaat cacagactta taagggagat
     2821 actcgcattg gtgtgcgctc acgacaggta aaaaaaaacc tgccatcgct ggcaggtttt
     2881 ttatgactaa aaaacgaaat taatcgtctt tcggtgcgcg gttggcgcgg cgacgatcgt
     2941 tttccgtcag gtgacgttta cgaatacgga tagaggtcgg agtcacttct accagttcgt
     3001 catcatcgat gaactccaga gcttgttcca gagtcatgcg gataggcgga accagaacaa
     3061 cagcttcgtc agtaccggaa gcacgcatgt tggtcagttt cttaccggtc aggcagttta
     3121 cagtcaggtc gttagagcgg ctatgaatac cgataatctg accttcgtaa acttctgcac
     3181 cgtgaccgag gaacagctta ccgcgatcct gcagaccgaa cagcgcgaac gcgacagctt
     3241 taccctgacc gttagagatc agtacaccgt tctgacgctg acccacttcg cccggacgta
     3301 cgtcgtcgta gtggctgaag gtggagtaca gcagaccagt accggaagtc atggtcatga
     3361 actcagaacg gaagccaatc agaccacggc ttgggatcac gtagtcgaga cgtacgcggc
     3421 ctttaccgtc tggattcatg tttttcaggt cgcctttacg ttcgcccagc gcctgcatca
     3481 cagaaccctg atgctgttct tcaacgtcca gcgtcacgtt ttcatacggc tcttgtttac
     3541 gaccgtctat ttcacggaag attactttcg gacgggatac cgccagttcg aaaccttcac
     3601 gacgcatgtt ttcgataaga acagagaggt gcagttcacc acgaccagaa acgcggaacg
     3661 catcggcgtc ttcggtttct tctacgcgca gcgcaacgtt gtgtaccagt tctttgttca
     3721 gacgatcgag gatctgacga gacgttacga acttaccttc tttaccgcag aacggcgagg
     3781 tgttaacgca gaagaacata gaaacggtcg gctcatcaac ggagagtgcc ggcagcgctt
     3841 caacgttttg cgtgtcgcaa acggtgtcag aaatgttcag ttcgcccagg cccgtgatcg
     3901 ccacgatatc gccagcttcc gccagatcgg tttcgatacg ttccagaccg aggtggccca
     3961 gcactttacc gactttcgcg ttacgggttt tgccttcgct atcgatgata gtaacctgct
     4021 ggttcggctt cactttacca cgcttgatgc ggccaatgcc gataacgcca acatagctgt
     4081 tgtaatcgag ctgagaaatc tgcatctgga acggaccgtc aaggtcaacg tccggcgcag
     4141 gaacgtggtc aacaatcgcc tgatacagcg gggtcatgtc ttccgccata tcttcgtggt
     4201 ccagacccgc gataccgttc agcgcagaag cgtaaacgat cgggaagtcc agctgctcgt
     4261 cggtcgcgtc gaggttaacg aacagatcga atacctgatc cacaacccaa tcaggacgcg
     4321 cgccagggcg gtcaactttg ttgataacaa caatcggctt caggccgtaa gcaaacgctt
     4381 ttttggttac gaagcgcgtt tgcggcatcg ggccgtcaaa tgcgtcaacc accagcagca
     4441 ctgagtctac catggacatt acacgttcaa cttcaccacc gaagtcggcg tgccccgggg
     4501 tatcaacgat gttgatacgg taatcattcc atttgatagc ggtgtttttc gcgaggatgg
     4561 taatcccacg ctctttctcc aaatcgttgg agtccatcac gcgctcttgg gtttcggcac
     4621 gagagtcgaa cgtaccggat tgttggagca gcttgtctac cagggtggtt ttaccatggt
     4681 ctacgtgcgc gatgatggcg atattacgca atttttcgat cacaactttg cctcaggcat
     4741 tagaaatagc gcgttattgt acacggatta atcgcactac aaaacaggat cacaaacatc
     4801 ctccgcaaac aagtattgca gagtcccttt gtgatcgctt tcacggagca taaaaagggt
     4861 tatccaaagg tcattgcacc aatatggtgc ttaatgtttc cattgaagca ctatattggt
     4921 gcaacattca catcatggtg cagccctttt gcacgatgtt aagcatgata acgcctttta
     4981 ggggcaattt aaaagttggc acagatttcg ctttatcttt tttacggcga cacggccaaa
     5041 ataattgcag atttcgttac cacgacgaca atgaccaatc caggagagtt aaagtatgtc
     5101 cgctgaacac gtactgacga tgctgaacga gcacgaagtg aagtttgttg atttgcgctt
     5161 caccgatacc aaaggtaaag aacaacacgt cactatccct gctcatcagg tgaatgctga
     5221 attcttcgaa gaaggcaaaa tgtttgacgg ctcctcgatt ggcggctgga aaggcattaa
     5281 cgaatccgac atggtgctga tgccagacgc atccactgca gtgattgacc cgttcttcgc
     5341 cgactccacc ctgattatcc gttgcgacat ccttgaacct ggcaccctgc aaggctatga
     5401 ccgtgacccg cgctctattg cgaagcgcgc cgaagattac ctgcgttcca ctggcattgc
     5461 cgacaccgta ctgttcgggc cagaacctga attcttcctg ttcgatgaca tccgtttcgg
     5521 atcatctatc tccggttccc acgttgctat cgacgatatc gaaggcgcat ggaactcctc
     5581 cacccaatac gaaggtggta acaaaggtca ccgtccggca gtgaaaggcg gttacttccc
     5641 ggttccgccg gtagactctg ctcaggatat tcgttctgaa atgtgtctgg tgatggaaca
     5701 gatgggtctg gtggttgaag cccatcacca cgaagtagcg actgctggtc agaacgaagt
     5761 ggctacccgc ttcaatacca tgaccaaaaa agctgacgaa attcagatct acaaatatgt
     5821 cgtgcataac gtagcgcacc gcttcggtaa aaccgcgacc tttatgccaa aaccgatgtt
     5881 cggtgataac ggctccggta tgcactgcca catgtctctg tctaaaaacg gcgttaacct
     5941 gttcgcaggc gacaaatacg caggtctgtc tgagcaggcg ctgtactaca ttggcggcgt
     6001 aatcaaacac gctaaagcga ttaacgccct ggcaaacccg accaccaact cttataagcg
     6061 tctggtcccg ggctacgaag caccggtaat gctggcttac tctgcgcgta accgttctgc
     6121 gtctatccgt attccggtgg tttcttctcc gaaagcacgt cgtatcgaag tacgtttccc
     6181 agatccggca gctaacccgt acctgtgctt tgctgccctg ctgatggccg gtcttgatgg
     6241 tatcaagaac aagatccatc cgggcgaagc catggacaaa aacctgtatg acctgccgcc
     6301 agaagaagcg aaagagatcc cacaggttgc aggctctctg gaagaagcac tgaacgaact
     6361 ggatctggac cgcgagttcc tgaaagccgg tggcgtgttc acggatgaag cgatcgatgc
     6421 gtacatcgct ctgcgtcgcg aagaagatga ccgcgtgcgt atgactccgc atccggtaga
     6481 gtttgagctg tactacagcg tctaatagtt gaagttgtac tacccggcgc acaacgccgg
     6541 gatttagttg ccgtggaaac tttcagccca tctctgcatg ggcttttttc tccgtcaatt
     6601 ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct
     6661 gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact
     6721 cgctgattaa cagtattttg ttaattgatg acaatctggc gatccattac gccaaccccg
     6781 ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacgccg ttaccggaac
     6841 tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag
     6901 gctttaccga taacgaagtg acgctggtca tcgatggtcg ctcgcatatc ctttctgtga
     6961 cggcccagcg tatgccggac ggcatgatcc tgctggagat ggcaccgatg gataaccagc
     7021 gccgcttaag ccaggaacaa ctacaacatg ctcaacaagt agctgcccgt gatttagtgc
     7081 gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagttac
     7141 tcagcaaagc gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg
     7201 cggaccggct gcgaaatttg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg
     7261 ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc
     7321 cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc
     7381 cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc
     7441 cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg
     7501 agcgctaccg gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc
     7561 atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg
     7621 gcttatccat cgcccgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt
     7681 ggccaggtca taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat
     7741 gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg
     7801 tgcgctcgct ggagcgggtt taacctgtac gacatttgag aacggcgcgg aagtactgga
     7861 ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga
     7921 cgggctggcg ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat
     7981 gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta
     8041 tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca
     8101 ttaccaggaa cagcagcagc cgcgtaatat tcagcttaac ggcccaacga ccgatatcat
     8161 cggcgaagcg caagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc
     8221 tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct
     8281 gcatcgccac agtccgcgca ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc
     8341 aaaagatttg atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa
     8401 taccattcgt caggggcgtt ttgaacaggc cgatggcggc acgttatttc tcgacgaaat
     8461 tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt
     8521 ttatcgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca
     8581 ccagaatctt gaacagcggg tgcaggaagg taagttccgt gaggatctgt tccaccgcct
     8641 gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct
     8701 ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttgctgca
     8761 tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga
     8821 aaacacctgc cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt
     8881 gcccggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag
     8941 ttgggcgaca cttttagcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct
     9001 gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac
     9061 gcaggggcat aaacaggaag cggcgcggct actcggctgg ggccgcaaca ctctgacgcg
     9121 taagttaaaa gagctgggga tggagtgatt cacggcttgt gtgtaaagat tgattattga
     9181 gcgcaaattg ctggtatttt gcgctttact gttccgataa gttcagtatg atcttgcccg
     9241 gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt
     9301 tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcga tgattgggct
     9361 gtttagctga agctcgttgt ttatgccgga tgcggagaaa gtcgtaggcc tgataagacg
     9421 cgaccagcgt cgcatcaggc atcatgctca gatgcctgat gcggcgtaaa cgccttatcc
     9481 ggcctacaaa agcaataatt tcaacaggtt ttgttgccca gcgcatcagg caatcttgtt
     9541 ctcagcacat tgatggcgct ttcgttttct actttgtaaa cgaagcgcca ttcactcttt
     9601 aaatcacccg tgagaactgc tgcatccgcg ctttctggcg cagataggta tcaaagcaca
     9661 tgcaaatgtt gcggatcagc aagcgacctt tcgccgtcac ctgtattccc ttctcatcca
     9721 catccaccag cccatctttt gctaacgggg cgagcagctt gagatcttcc gcaaagtaat
     9781 cagcgaagtg caaatcccac tgtttctcaa gaggggcgta atccagacgg aagttgcaga
     9841 tgagcgactt aatcacatcg cggcgaatac agtcatcacg cgttagcgca ataccacgcc
     9901 acagcgcatt gccttgttca tacacttgct gatagtactg cttcaactct ttctggttct
     9961 gcgcgtagca gtcgccaatc atgctgatgg cggaaacgcc catccccagc agatcggtat
    10021 cgccctgagt ggtgtagccc tggaagttac gatgcagcac gccttcacgc tgggccaccg
    10081 ccagctcgtc atcaggacgg gcaaagtgat ccataccgat aaactgatag cccgattgcg
    10141 tcaggaaggc gatggtttcc tgcaggatat cgagtttttg ctgcggactc ggcaggtcag
    10201 catctttgat tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag ttaaaaacgc
    10261 tcagacgatc ggggttcagc tccgccacac gtttcagggt aaaggcgaaa ctctccggcg
    10321 tctgtttcgg caggccgtaa atcaggtcga tgttggtgga ggtaaaacca atctcacgcg
    10381 catggttaag cagtgcaaag atgaactctt catcctgctc gcggttaacc agacgttgca
    10441 cttctttgtt gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat
    10501 gatcgagtac atccagttcg atttcccgcg gatcgacttc gatcgaaatc tccgcatcgg
    10561 tattgaaatg gaagttttcg cgcagcagct tcatcagacg gctgatttgc gctttattca
    10621 gatacgtcgg cgttccgccg ccccagtgca attggctgac gtgacgcccg gcgaacagcg
    10681 gtgcacgatg gacgatttct tgctccagcg cgtccagata ctgatcggcc ttgtgctgct
    10741 ggcgagtaac aatcttattg caaccgcaga agtaacaaag cttatggcag aacgggatat
    10801 gtacgtagag agataatgga cgctcaggat agcgcgccac ggcttgtaaa aacgcctgtt
    10861 cgccgaagtc ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat
    10921 agttatattt ctggatcagg gccagatccc agtcgatttg ctgtacagac atgctcactc
    10981 cttccgatgg cgtctctggc ggctacggcg taccggcgga tgcccgtttc gggtcatcag
    11041 ccggttgcgc agccactttt gtcgccgcga caaccgctgt agtttaacga ataaccacac
    11101 cagataacat ataaccggaa gggttatcag cagaacggga aggcccacgg cggcaatccg
    11161 ttagttgccc cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc
    11221 gtcatcgtca taagagaggc cgagtttctg catcaactca tcaatacgat ccagtttagc
    11281 atccacccag gattgctctt cggcgctcag ggtttcgcct gcttccagac gttccagcag
    11341 cgcatccaga cactcatccg tttccagtaa ttccaactcc gcctgcggtg aaagcatagg
    11401 tttctcactc ttcggtttgt gctgtttggt gactttttca gtcactccca atggaatagg
    11461 ggttttactg ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc ctgacgtggt
    11521 gtttccgccc gctgcgcggc tgcccggcgc atgaccacga cgttttttct gacgcttacg
    11581 gtcacgagct tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg catgaccttt
    11641 gctgcgtgag tttgaagatg atggtttcat aatggttcgt cttttagccg ttttattcag
    11701 tatagatttg cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc
    11761 tgtcgccttt tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca
    11821 acacaccctg tttttccatt agccggaaca atccgtgttg attccatttc cgtttaatta
    11881 cgtctccgga cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc
    11941 ctcatcctga gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt
    12001 tgctttaaac aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa
    12061 agcaattcac tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt
    12121 cgcataaaga ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg
    12181 ccgaaaaatc cgccttttat tatggttcag gtataatcgc cacaaagctt tgactgacgg
    12241 agacgaccgc tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg
    12301 atattcgcca cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg
    12361 caggtaaatc cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa
    12421 aaaccccagg gcgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg
    12481 ttgacttgcc tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc
    12541 gtgcgctcgg cgaatacctc gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg
    12601 atattcgcca tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca
    12661 atatcgccgt tctggtgctg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag
    12721 cgctattgaa tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg caggttgaaa
    12781 cgttttcttc gttgaagaaa caaggcgtag acaagctgag gcagaaactg gatacctggt
    12841 ttagcgagat gcagcctgta gaagaaacgc aggacggcga ataattttct tgccttaatg
    12901 cttgtgccgg atgtggcgta tccggcccgt aaattcaata gattacaatt ctaccgttgg
    12961 cctgataaac atagcgcatc aggcattacg gatcttttct ttcgcccaat aaaaaacgcc
    13021 ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa
    13081 aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt actctttttt
    13141 gttcagttca gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat
    13201 agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc
    13261 gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca
    13321 tccagacggg tacagttttc catcagttga tgaatctgct tcgcgacggc atcaacatca
    13381 tctttatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt
    13441 tgctcagcct gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg
    13501 gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg
    13561 ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagcccagc
    13621 tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag
    13681 taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg
    13741 aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg
    13801 ctggtgacgg tttccagtgg caaaccaaac acttctgccg ccgttgcccg gtggatatct
    13861 tttccttccg cgaatgcggt cagcaagcct ttatcacgcg agagatgcgc cataatgcgc
    13921 agttcaatct gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc
    13981 tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt
    14041 gacgataaac gtcccgttgc agttactgcc tggtgataag aggtatgcac acgcccggtt
    14101 ttcgggttga tcatcagcgg cagcttgtcg gtgtaagtcg acttcagctt cgccagacca
    14161 cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct
    14221 tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc ctgtttttca
    14281 aacagaatgg tttgcaactg cttggtggaa gaaaggttaa attcttcacc tgcaatttca
    14341 tgcgctttct tttccagctc agccagacgc aaggtgagct cttcagaatg attgtgcagc
    14401 actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg caccagcggc
    14461 atttcgatat tctcgaagac gttcaacggc cctttgtgtt tctgcagatc cggccacatt
    14521 ttcagatgca actgcaaggt gacatctgca tcttcggcgg cgtaacgtcc ggcctcttcg
    14581 agggcaatct ggttaaaggt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg
    14641 atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg
    14701 ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ttcaatgccg
    14761 taattcgcca gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca
    14821 tcttccagca gcggttttag caactcgagt gcacgctcgt gagagatttg atcgggcgca
    14881 tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat agcaaaagaa
    14941 agcccgacca ggttagcaga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat
    15001 accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatagtg
    15061 acgtagttgt cataagaaat caccgttgcc gtcacttctg gcgcttcgtc tgcaacactg
    15121 gtttcctgag gcttcgcggc tgtttttgcc cctttggcct gtaaccattt gcccacttcg
    15181 acatcagcag tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct
    15241 gccggttgct gcacttccag ttgttcacag gtcagctcca gttcaacatc ggttttaatc
    15301 gtcgccaact ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt
    15361 gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc
    15421 agcccgccaa gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca
    15481 ggaatgttat cagaggagtc acccatcagc gccaggaagt cgatgatcaa ttctggcggc
    15541 acgccgtact tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtattgata
    15601 agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgccagtgct aatcagcacc
    15661 ggacgcccgg ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg
    15721 ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcag aggttcgatt
    15781 tgtgctcgca gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca
    15841 tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc
    15901 atgatcagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg
    15961 ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca
    16021 aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg
    16081 ggtaaaggat gccacagaca ggatgaaaac atgagttttt caccgctttt cgtgaaaatc
    16141 ttttgtagat ctgctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta
    16201 tttacatcgc ttttaattta ttatcatgct ttttacagca tgatgtttgt ctttgttttt
    16261 catgcagtta aaaataaatc catccctaat aattgagata atgggatttt ttctaatgtg
    16321 gatagatatg aattattttt ctccttaagg atcatccgtt atttgggtca ttttctgcta
    16381 aaatctgcgc cttgcgcttc tttagtgcac tgtctatggc taaccttttg aataaattca
    16441 ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg
    16501 tcactatttt ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg
    16561 tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg
    16621 aaggtctggc ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg
    16681 aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata
    16741 ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc
    16801 aacagctggc ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta
    16861 tgaagcgtta ttcccgcgct tatttattac gtcatccgga gcgacgtggt caagatgttg
    16921 aaaccactcg gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg
    16981 ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact
    17041 tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt aaacaattcg
    17101 ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata
    17161 tgttaagcgg taaactgacg cggattgtcg tacatgtgga tttacagcct attgccgatg
    17221 agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga
    17281 actcattgtg gcaggaaaaa gaccggcttc tgacgtctct catgtcatca cagcgtcaga
    17341 ataagtaagt tttactcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa
    17401 acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt
    17461 acccggtgta taacgcagct gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa
    17521 catctgacct aatgtcatca tcccgctgat ttgtttatca gcctgctttt gcgctttttc
    17581 cgcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg
    17641 taacttcaca tcaaaattga gcgatttgat atctttgttt gtggatgatg aagatttggc
    17701 tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccatct
    17761 taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtgaatact ctttgaacaa
    17821 tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag
    17881 ctgcttctgc atcgcaatgt tatactggat gataaattgg cgcactgctg agggatcaat
    17941 agattcagca atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt
    18001 atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcct
    18061 ggtttttgca acgaaagcat cgatttgggc aacgtcttcc ccgtgattga taatgttaat
    18121 ctgattaaat ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgcgccag
    18181 agaatcaatg gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat
    18241 atcttcgact ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt
    18301 aaactggccc tcaccaaaag aaaattttcc atactctgca ggggcaacat ctaatgtcgt
    18361 agagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc
    18421 ggcgataaat agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag
    18481 tgctgggatg acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc
    18541 cacatcaaat acaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcgaa
    18601 tgtcatttgc atttgaaaac gggaattaaa aaagcctttg tcaaagtttt tataacttaa
    18661 cgtcatatcg taggcatgtt cacctttctt tttcgcatcg ttaaaatctt taataaactt
    18721 ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc
    18781 taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac
    18841 agcaaaactt tgaatatcca cttatgctga aataatgtcc agcggcagga tgcattatca
    18901 gaatgtattt atatttatta aataataaaa aaagcccgtg aatattcacg ggctttatgt
    18961 aatttacatt gaattatttt tcctcggaca gatatttcac cgtatcagca tactgctgaa
    19021 caaaaacatc catattgctg gtatccatac cctgcggatt cagctgatat ttaccgttaa
    19081 caaacatcgc cggaacacca cgcaattgca catcagctgc ggctttttcc tgctgagcga
    19141 ccagagattt caccacgaag ctgttccacg ccgcgtcgta ctcttcacct ttaatacctg
    19201 cattgataaa tacatcgcgg atatcagatg ctgaacgaat ggtctgggtt ttctgtacgc
    19261 cttcaaacag cggaacagtc actttgtctt ccacgcccag cgccatcgcc acagcccatg
    19321 cctgagtcag atctttgccc aggtcaccac ccatgaagtt gacgtggtat ttagtcatct
    19381 tcacgccttc cggcagtttt ttcttcacat tatcagaaat atgcagaact tcttcaaact
    19441 gatagcagtg cgggcagaag aaagagaaaa actccagcac ttgcggcgcg ccagctaccg
    19501 gtttttccag ggtagtgtac tgtttaccat cttcatactg cgccgccgat gcgctaaacg
    19561 ctaaaactaa accagccagc gccagccaaa tctttttcat gatctactct ctccgattaa
    19621 tacataggtg ttaattgcaa agggggttct tgtagaactt ttgcctgttc tataaaagtc
    19681 gccgtctgtc gtagccagta atcttccccg gttaaccacg ggaaattttt cgggaacgcg
    19741 ggatcagccc aatgccgcat tagccaggca agataataaa ccaaacgcat ggcgcgtaaa
    19801 ggttcaatca gtccgatttc agcggtgtcg aactcgctaa attcttcata agcttcaata
    19861 atagtttcca gttgcatccg ctgctgggct ttatcgccat tgagcaacat ccacaaatcc
    19921 tgaatagctg gaccattacg tgcatcatcc agatcaacaa acattggacc atcgcgccag
    19981 agaatattcc cggcgtggca gtctccatgt agccgcagaa cggtgaaatc ttcccgccag
    20041 tgtgctgtaa cggcggcgat cagctcatct gtcgctttca gaaatgccgc tttcaacccg
    20101 gaaggtatca gtgtagcgtc ctcaaacagc ttgcgaggct caatgagata ttcattcaga
    20161 ccgatggtcg ggcgatgaat aaaaagctgt ttgcgccccg tctggtgcat acgccctaaa
    20221 taacgcccaa ccgcttccat ctgatcgata ttatcagctt cgaactggcg accaccgacg
    20281 cttggaaaaa cagcgaaata aaatccctga tgattcaata aagtctgacc gttaaaggcc
    20341 acaggtgctg cgaccggaac ttcatcattt accagctgca acgcaaattg atgttcttcg
    20401 aggatttgat cggctgtcca acgttcaggg cgataaaatt tgacgacaaa acgtcgacgc
    20461 tcttcgtcct gaaattgata gacacggttt tcatagctgt taagcggggt aagaccggaa
    20521 tccacccgga tcccttgctt aaacagagcg tccatgatgg tatccgggtg tagtgtctgg
    20581 aaagtaaaag cgctgttgtt catcccatca tccggaaaac attacgaatg atgaaggata
    20641 tcatttcacg atgattttgg tggcgccgct tacaagcttt tactctttaa ttacgccgcg
    20701 agcgcgtaac agcgccgttt tgaagtcttc ttcataatct ttctgcaaac ccgggatcac
    20761 cgcatctttc gcggaatcac gcattttcag gtgatagatc agaatgtcat ccgtcaaatc
    20821 cgccagttcg ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt gcaacaagtt
    20881 aaagtctggc tctttttgcc aggctggctg gaggagttca ataacttcat tcagacgttt
    20941 acatttcata gtaatgctcc ttactcttga gacagacacg ttagcagggt caatcccaca
    21001 ataaaagagg cgatatcggt gaatctaatg acgacgataa caggcgttgt gctggcaggc
    21061 ggtaaagcca gacgaatggg cggcgtagat aaaggattgc tggacttaaa tggcaaaccg
    21121 ctgtggcaac atgtcgctga cgcgcttaag acgcagctct ctcacgtcgt ggttaatgct
    21181 aatcgtcatc aggaaatcta tcaggcaggc ggtctgaaag tgattgaaga ttcactggcg
    21241 gattacccag gccctctggc aggaatgctt tcagtaatgc agcaggaagc tggtgagtgg
    21301 tttttgtttt gtccgtgcga tacgccttac attccccatg atttagccgc ccggcttaat
    21361 catcagcgca aagatgcgcc tgtcgtgtgg gtccatgacg gtgaacgcga tcacccgact
    21421 attgctctgg taaaccgcgc tattgagcct ttattactgg aatatctgca agcaggagaa
    21481 cgccgggtaa tggcatttat gcgtctggct ggcggtcatg cggttgattt cagcgatcat
    21541 aaagatgcat ttgttaacgt gaatacgcca gaggagcttg cccgatggca ggaaaaacga
    21601 tgataccgtt actcgccttt gccgcgtgga gtggtaccgg aaaaactacg cttttgaaaa
    21661 aactgatccc ggcattatgc gccagaggga tccgtccggg gctgattaag catacgcacc
    21721 atgatatgga tgttgataag ccaggcaaag atagctatga gctgcgcaag gctggcgcgg
    21781 tacaaaccat cgttgccagc cagcaacgat gggccttgat gacggaaaca ccagacgaag
    21841 aagagctgga tctacatttt ctcgcaagtc gaatggatac ctcaaagctg gatttgattc
    21901 tggtcgaagg gtttaagcat gaagagatcg caaagattgt gctgtttcgc gatggagccg
    21961 gacatcgacc tgaagaatta gtgatagaca ggcatgttat tgctgtagcc agtgatgtgc
    22021 cgcttaatct tgatgtcgca ttactggata ttaatgatgt tgaggggctg gctgattttg
    22081 tggtggagtg gatgcaaaag cagaacggat aagcaagagt aggctggatc agacgc
//
LOCUS       DAJTYL010000064        21322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYL010000064 DAJTYL010000000
VERSION     DAJTYL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.064"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            4..534
                     /gene="rppH"
                     /locus_tag="OO572_002386"
     CDS             4..534
                     /gene="rppH"
                     /locus_tag="OO572_002386"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6661364.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            547..2793
                     /gene="ptsP"
                     /locus_tag="OO572_002387"
     CDS             547..2793
                     /gene="ptsP"
                     /locus_tag="OO572_002387"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6661365.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            2944..3819
                     /gene="lgt"
                     /locus_tag="OO572_002388"
     CDS             2944..3819
                     /gene="lgt"
                     /locus_tag="OO572_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6661366.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            3826..4620
                     /gene="thyA"
                     /locus_tag="OO572_002389"
     CDS             3826..4620
                     /gene="thyA"
                     /locus_tag="OO572_002389"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6661367.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            4804..5274
                     /locus_tag="OO572_002390"
                     /pseudo
     CDS             4804..5274
                     /locus_tag="OO572_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            5265..5828
                     /locus_tag="OO572_002391"
     CDS             5265..5828
                     /locus_tag="OO572_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6661368.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            5825..6232
                     /locus_tag="OO572_002392"
     CDS             5825..6232
                     /locus_tag="OO572_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6661369.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            6217..6567
                     /locus_tag="OO572_002393"
     CDS             6217..6567
                     /locus_tag="OO572_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6661370.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            6580..9948
                     /gene="recC"
                     /locus_tag="OO572_002394"
     CDS             6580..9948
                     /gene="recC"
                     /locus_tag="OO572_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6661371.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            10124..13012
                     /gene="ptrA"
                     /locus_tag="OO572_002395"
     CDS             10124..13012
                     /gene="ptrA"
                     /locus_tag="OO572_002395"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6661372.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            13005..16547
                     /gene="recB"
                     /locus_tag="OO572_002396"
     CDS             13005..16547
                     /gene="recB"
                     /locus_tag="OO572_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6661373.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            16547..18373
                     /gene="recD"
                     /locus_tag="OO572_002397"
     CDS             16547..18373
                     /gene="recD"
                     /locus_tag="OO572_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6661374.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18435..19766)
                     /gene="argA"
                     /locus_tag="OO572_002398"
     CDS             complement(18435..19766)
                     /gene="argA"
                     /locus_tag="OO572_002398"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6661375.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            19998..21251
                     /gene="amiC"
                     /locus_tag="OO572_002399"
     CDS             19998..21251
                     /gene="amiC"
                     /locus_tag="OO572_002399"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6661376.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         4979 a         5329 c         5964 g         5050 t
ORIGIN      
        1 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
       61 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
      121 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
      181 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
      241 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
      301 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
      361 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
      421 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
      481 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
      541 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
      601 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
      661 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
      721 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
      781 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
      841 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
      901 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
      961 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     1021 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     1081 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     1141 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     1201 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     1261 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     1321 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     1381 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggtgattta
     1441 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     1501 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     1561 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     1621 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     1681 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     1741 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     1801 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     1861 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagctgtat tgatggcatc
     1921 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     1981 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     2041 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     2101 tgcctgggtt ggcgtgggat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     2161 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     2221 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     2281 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     2341 ccgtcaatgg tatttatgct gccgcatctg gcaaagaggg tcgatttcat ctctgttggc
     2401 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggcgaac
     2461 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     2521 gaaatacatg gtatcgatct tcgtttgtgc ggtgaaatgg cgggcgatcc catgtgcgtg
     2581 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     2641 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     2701 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     2761 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     2821 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     2881 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     2941 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     3001 cccgtggcgc ttcactggta cggcctgatg tatctggtgg ggttcatttt tgcaatgtgg
     3061 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     3121 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     3181 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     3241 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     3301 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     3361 ggtgccgggc gtctgggcaa ctttattaac ggtgaattgt ggggccgcgt tgacccgaac
     3421 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     3481 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     3541 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     3601 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     3661 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     3721 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     3781 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     3841 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     3901 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     3961 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     4021 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     4081 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     4141 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     4201 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     4261 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     4321 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     4381 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     4441 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     4501 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     4561 gactttgaga ttgaaggcta cgatccacat ccgggcgtta aagcgccggt ggctatctaa
     4621 ttacgaaaca tcctgccaga gccgacgcca gtgtgcgtcg gtttttttac cctccgttaa
     4681 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     4741 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     4801 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     4861 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     4921 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     4981 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     5041 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     5101 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     5161 gcatgggccg tgcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     5221 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     5281 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     5341 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     5401 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     5461 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     5521 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     5581 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     5641 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     5701 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     5761 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     5821 acctgtgaac cgcgaaaaag gtgtttcgtc actggctctg gtcctgatgc tgctggtttt
     5881 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     5941 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     6001 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     6061 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     6121 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     6181 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     6241 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgct gatggtgctg
     6301 atggtgctga tggtgatgat tgtcactgcg ttatcgggta tccagcgaac atcaatgaac
     6361 agtctcgcca gcagaaacca gtaccaaaag ctctggcggc atggttggca gcaaacgcaa
     6421 ctgcgcgcga tttcgccacc tgccaactgg caggtcaacc gaatgcagac atcgcaggcg
     6481 ggatgtgtca gcatcagcgt tacgctagtt tcacccgggg gcagagaagg cgagatgacc
     6541 cgcctgcatt gcccgaattg tcagtagtca ggagccgcta tgttaagggt ctaccattcc
     6601 aatcgtctgg acgtgctgga agcgttgatg gagtttattg tcgaacgcga acggctggac
     6661 gatcctttcg aaccagagat gattctggtg caaagtaccg gtatggcgca gtggctgcaa
     6721 atgaccctgt cgcaaaagtt tggtattgcg gcaaacattg attttccgct gccagcgagc
     6781 tttatctggg atatgttcgt ccgggtgtta ccggaaatcc ccaaagagag cgcctttaac
     6841 aaacagagca tgagctggaa actgatgact ctgctgccgc aattgctgga gcgcgaagac
     6901 tttaccctgt tgcggcatta tctgactgac gatagcgaca agcgaaaact gttccagctt
     6961 tcctcaaaag cggcggacct gtttgaccag tatctggtct atcgtccgga ctggctggca
     7021 cagtgggaaa caggacattt ggttgaaggg ctgggagaag cacaggcctg gcaagccccg
     7081 ttgtggaagg cgctggtgga atatacccat gaactcggac aaccgcgctg gcaccgcgcg
     7141 aatctctatc agcgctttat cgaaacgctg gagtccgcga cgacctgccc gccggggtta
     7201 ccttcgcgcg tctttatatg cggtatttcc gcgttaccgc ctgtttatct ccaggcgctc
     7261 caggcgctgg gtaaacatat tgaaatccat ctcctgttta ccaacccctg ccgttattac
     7321 tggggcgata ttaaagatcc tgcttatctg gcgaaactgc tgactcgtca gcgtcgacac
     7381 agttttgaag atcgcgaatt gccgctattt cgcgatagcg aaaatgccgg acagctcttt
     7441 aacagcgatg gtgaacagga tgtcggcaac tcgctgctgg cctcatgggg gaagcttggg
     7501 cgcgactaca tttatctcct ttctgacctg gagagcagcc aggagctgga cgcttttgtc
     7561 gatgtgacgc cagataacct gctgcataac attcagtctg acattctgga actggaaaac
     7621 cgcgccgttg cgggtgtgaa catcgaagag ttttcccgta gcgataacaa acgcccgctt
     7681 gatccactgg atagcagtat cacattccac gtttgccata gcccgcagcg tgaagttgaa
     7741 gttttacacg atcgcttgct ggcgatgctg gaggaagccc cgacacttac tccgcgcgac
     7801 atcatcgtga tggtggctga tatcgacagc tacagtccgt ttattcaggc agtgtttggt
     7861 agcgcacctg cggatcgtta cctgccttac gccatttccg accgtcgtgc gcggcagtca
     7921 catccggtac tggaagcgtt tatcagcctg ttatcactgc ctgacagtcg ctttgtgtca
     7981 gaggatgtgc tggcgctgct ggatgtgccg gtgctggcgg cgcggtttga catcaccgaa
     8041 gaagggctgc gttatttacg ccagtgggtc aacgaatccg gcattcgttg ggggatagat
     8101 gacgacaacg ttcgcgagct ggaactcccc gccaccggac aacacacctg gcgatttggc
     8161 ctgacgcgta tgttgctggg ctacgcgatg gagagcgcgc agggcgagtg gcaatcggtt
     8221 ctaccttatg atgaatcgag cggcttaatt gcagaactgg tggggcatct ggcttcactg
     8281 ctaatgcagc taaacatctg gcgtcgcggg ctggcacagg agcgtccgct agaagagtgg
     8341 ttgccggttt gtcgcgatat gctcaacgcc ttcttcctgc cggatgcgga aaccgaagcg
     8401 gcgatgacgc tgatcgaaca acaatggcag gcgattatcg ccgaaggttt aggtgcgcag
     8461 tatggcgacg cggtgccgct gtcactattg cgtgatgaac tggcacagcg tctggatcaa
     8521 gaacgtatca gccagcgttt tctcgccgga ccggttaaca tttgtactct gatgccaatg
     8581 cgttcaattc cgttcaaagt ggtttgcctg ctgggaatga acgacggcgt ttatccacgt
     8641 cagcttgcgc cattgggctt tgacctgatg agccagaaac cgaagcgtgg cgaccgtagc
     8701 cgtcgcgatg acgaccgcta cctgttcctg gaagcgttaa tttccgcgca gcaaaaactc
     8761 tatatcagct atatcgggcg ttccattcag gataacagtg aacgtttccc gtcggtactg
     8821 gtgcaggaac tgatcgacta catcgggcaa agtcattatc taccgggcga tgaagcgctc
     8881 aactgtgatg aaagcgaggc aagggtaaaa gcgcatctta cttgcctcca tacccggatg
     8941 ccgtttgatc cgcaaaacta ccagccaggc gaacgacaaa gctatgctcg tgaatggcta
     9001 cctgcggcca gccaggctgg taaagcacat tctgaatttg ttcagccgct gccgtttacc
     9061 ttaccggaaa ccgtgccgct ggaaacgcta caacgattct gggcacatcc ggtgcgggcg
     9121 ttcttccaga tgcgtttgca ggtgaacttc catactgaag acagcgaaat cccagacacc
     9181 gagccattta ttctggaagg acttagccgt tatcaaatca atcagcagtt attgaatgca
     9241 ctggttgagc aggatgatgc cgaacgcttg ttccgccgct tccgggcggc aggggattta
     9301 ccgtatggcg cttttggtga aattttctgg gaaacacagt gccaggagat gcagcagctt
     9361 gccgacagag tcattgcctg tcgccagccg gggcagagta tggaaattga tctcgcctgc
     9421 aacggtgtgc agataactgg ctggttgccg caggtgcagc cggatggcct gttgcgctgg
     9481 cgtccctctt tattaagtgt ggcgcaggga atgcaacttt ggctggaaca ccttgtctac
     9541 tgtgccagcg gtggtaatgg cgaaagtcgc ctttttctac gcaaagacgg tgagtggcgt
     9601 tttccgccgc ttgcagccga acaggctttg cattacctct cacaactgat tgaggggtat
     9661 cgtgaaggaa tgtccgcgcc attgctggtg ttacctgaaa gtggcggcgc gtggctaaaa
     9721 acctgttatg acgcgcaaaa cgatgccatg ctggatgacg attccacgtt gcaaaaagcc
     9781 cgtacgaaat tccttcaggc ttacgaaggc aacatgatgg tgcgtggcga aggtgatgat
     9841 atctggtatc aacggctctg gcggcaatta acaccagaga caatggaggc catcgttgaa
     9901 cagtcgcaac gtttcctgtt accgctgttt cgctttaatc agtcatgagt gttgtataaa
     9961 aattgcgcaa tctatctgct tactttatga tgcgcaccag tcacggactg atggttatat
    10021 aaacataggc tgactcgtgc agcacaagat taaattctgg cagatgattt gcgttaacct
    10081 gttgaatctg tccagaaaat aaagttgatt atgaggtccg tgaatgcccc gcagcacctg
    10141 gttcaaagca ttattgttgt ttgttgccct ctgggcacca ttaagtcagg cagaaacggg
    10201 atggcagccg attcaggaaa ccatccgtaa aagtgataaa gataaccgcc agtatcaggc
    10261 tatacgtctg gataacggta tggtggtctt gctggtttct gatccgcagg cagttaaatc
    10321 gctctcggcg ctggtagtgc ccgttgggtc gctggaagat cccgaggcgt accaggggct
    10381 ggcacattac cttgaacata tgagtctgat ggggtcgaaa aagtacccgc aggctgacag
    10441 tctggccgaa tatctcaaaa tgcacggcgg tagtcacaat gccagcactg cgccgtatcg
    10501 cacggctttc tatctggaag ttgagaacga cgccttgccc ggtgcggtag accgcctggc
    10561 cgatgctatt gcagaaccct tgctcgacaa gaaatacgcc gaacgtgagc gtaatgcagt
    10621 gaacgctgaa ttaaccatgg cgcgtacgcg tgacgggatg cgcatggcac aggtcagcgc
    10681 agaaaccatt aacccggcac accccggttc aaagttttct ggtggtaacc tcgaaacttt
    10741 aagcgacaaa cctggtaatc cggtgcagca ggcgctgaaa gatttccacg agaagtacta
    10801 ttccgccaat ttgatgaagg cggttattta cagtaataaa ccgctgccgg agttggcaaa
    10861 aatggcggcg gacacctttg gtcgcgtgcc gaacaaagag agcaaaaaac cggaaatcac
    10921 cgtgccggta gtcaccgacg cgcaaaaggg cattatcatt cattacgtcc ctgcgctgcc
    10981 gcgtaaagtg ttgcgcgttg agtttcgcat cgataacaac tcagcgaagt tccgtagtaa
    11041 aaccgatgaa ttgattacct atctgattgg caatcgcagc ccaggtacac tttctgactg
    11101 gctgcaaaag cagggattag ttgagggcat tagcgccaac tccgatccta tcgtcaacgg
    11161 caacagcggc gtattagcga tctctgcgtc tttaactgat aaaggcctgg ctaatcgcga
    11221 tcaggttgtg gcggcaattt ttagctatct caatctgtta cgtgaaaaag gcattgataa
    11281 acaatacttc gatgaactgg cgaatgtgct ggatatcgac ttccgttatc cgtcgatcac
    11341 ccgtgatatg gattacgtcg aatggctggc agataccatg attcgcgttc ctgttgagca
    11401 tacgctggat gcagtcaata ttgccgatcg gtacgatgct aaagcagtaa aggaacgtct
    11461 ggcgatgatg acgccgcaga atgcgcgtat ctggtatatc agcccgaaag agccgcacaa
    11521 caaaacggct tactttgtcg atgcgccgta tcaggtcgat aaaatcagcg cacaaacttt
    11581 cgccgactgg cagaaaaaag ccgccgacat tgcgctctct ttgccagagc ttaaccctta
    11641 tattcctgat gatttctcgc tgattaagtc agagaagaaa tacgaccatc cagagctgat
    11701 tgttgatgag tcgaatctgc gcgtggtgta tgcgccaagc cgttattttg ccagcgagcc
    11761 caaagctgat gtcagcctga ttttgcgtaa tccgaaagcc atggacagcg cccgcaatca
    11821 ggtgatgttt gcgctcaatg attatctcgc agggctggcg cttgatcagt taagcaacca
    11881 ggcgtcggtt ggtggcataa gtttttccac caacgctaac aacggcctta tgattaatgc
    11941 taatggttac acccagcgtc tgccgcagct gttccaggca ttgctcgagg ggtactttag
    12001 ctataccgct acggaagagc agcttgagca ggcgaagtcc tggtataacc agatgatgga
    12061 ttccgcagaa aagggtaaag cgtttgagca ggcgattatg cccgcgcaga tgctctcgca
    12121 agtgccgtac ttctcgcgag atgaacggcg taaaattttg ccctccatta cgttgaaaga
    12181 ggtgctggcc tatcgcgacg ccttaaaatc aggggctcga ccagagttta tggttatcgg
    12241 caacatgacc gaggcccagg caacaacgct ggcacgcgat gtgcaaaaac agttgggcgc
    12301 tgatggttca gagtggtgtc gaaacaaaga tgtagtggtc gataaaaaac aatccgtcat
    12361 ctttgaaaaa gccggtaaca gcaccgactc cgcactggca gcggtatttg taccgactgg
    12421 ctacgatgaa tacaccagct cagcctatag ctctctgttg gggcagatcg tacagccgtg
    12481 gttctacaat cagttgcgta ccgaagaaca attgggctat gccgtgtttg cgtttccaat
    12541 gagcgtgggg cgtcagtggg gcatgggctt ccttttgcaa agcaatgata aacagccttc
    12601 attcttgtgg gagcgttaca aggcgttttt cccaaccgca gaggcaaaat tgcgagcgat
    12661 gaagccagat gagtttgcgc aaatccagca ggcggtaatt acccagatgc tgcaggcacc
    12721 gcaaacgctc ggcgaagaag catcgaagtt aagtaaagat ttcgatcgcg gcaatatgcg
    12781 cttcgattcg cgtgataaaa tcgtggccca gataaaactg ctgacgccgc aaaaacttgc
    12841 tgatttcttc catcaggcgg tggtcgagcc gcaaggcatg gctattctgt cgcagatttc
    12901 cggcagccag aacgggaaag ccgaatatgt gcatcctgaa ggctggaaag tgtgggagaa
    12961 cgtcagcgcg ttgcagcaaa caatgcccct gatgagtgaa aagaatgagt gatgtcgccg
    13021 agacactaga tcctttgcgc ttgcccttac agggcgagcg cctgattgaa gcctctgccg
    13081 gcacaggcaa aacctttacg attgcggcgc tctatttgcg cctgttactt ggactaggcg
    13141 gttccgccgc ctttccccgc ccgctgatcg ttgaagaact gctggtggtc acctttaccg
    13201 aggctgccac ggcagaattg cgcggtcgta tccgtagcaa tatccacgag ttgcgcatcg
    13261 cctgtctgcg tgaaaccacc gacaatccac tgtacgaacg cctgctggaa gagatcgacg
    13321 ataaagcgca agccgcgcag tggttgttgt tagccgaacg gcagatggat gaagcggcag
    13381 tctttactat tcacggcttt tgccagcgca tgctcaacct gaatgccttt gaatccggca
    13441 tgctgtttga gcagcagctg attgaagatg agtctctgct acgctaccag gcctgcgccg
    13501 atttctggcg tcgccactgc tacccgctgc cgcgtgaaat agcccaggtc gtctttgaaa
    13561 cctggaaagg gccgcaggcg ttgctgcgcg atattaatcg ttatctgcaa ggcgaaccgc
    13621 cggttatcaa agcaccgccg cccgatgatg aaacgctggc ttcccgccac gcgcaaattg
    13681 tggcgcgtat tgatacggta aaacagcagt ggcgcgacgc agtgggtgaa ctggatgcgc
    13741 tgatcgaatc ttctggtatt gatcgacgca agtttaaccg tagcaatcag gctaaatgga
    13801 tcgaaaagat cagcgcctgg gcagaagaag agacaaacag ttatcagttg ccggagtcgc
    13861 tggaaaaatt ctcccagcgt ttcttagaag atcgcacgaa ggccgggggg gaaaccccgc
    13921 gacatccact gtttgaggcg atcgatcaac tgcttgcaga accattgtcg atccgcgatc
    13981 tgttgatcac ccgcgcattg gctgagatcc gcgaaacagt agcgcgtgaa aaacgccgcc
    14041 gtggcgaatt gggttttgat gacatgttaa gtcggctcga ttccgcgctg cgtagcgaaa
    14101 gcggcgaggt gttggcagcg gcgatccgta cgcgattccc ggtggcaatg atcgatgaat
    14161 ttcaggatac cgacccccag cagtaccgaa tttttcgccg tatctggcac catcagccgg
    14221 aaaccgcatt gttgctgatt ggcgacccga agcaggccat atatgcattc cggggtgcgg
    14281 atatcttcac ttatatgaag gcgcgtagtg aagttcatgc ccactacact ttagacacca
    14341 actggcgctc cgcacctgga atggtgaaca gtgtgaacaa gcttttcagc cagactgatg
    14401 acacgttcat gtttcgcgaa ataccgttta ttccagtgaa atcagccggg aaaaatcagg
    14461 cgttacgttt tgtatttaaa ggtgaaacgc agcctgcgat gaaaatgtgg ctgatggaag
    14521 gcgaaagctg cggcgttggc gactatcaaa gtaccatggc gcaggtatgt gctgcgcaaa
    14581 tccgcgactg gctacaagcc ggacagcggg gcgaagcgtt gctgatgaac ggcgacgacg
    14641 cgcgtccggt gcgtgcttcg gacatcagcg tgctggtgcg cagccgccag gaggccgccc
    14701 aggtgcgcga tgccttaacg ttgctggaaa tcccttccgt ttacctttcg aaccgcgaca
    14761 gtgtttttga aactctggaa gcgcaggaaa tgctttggtt gttgcaggcg gtgatgacgc
    14821 ccgaacgtga gaacaccctg cgcagtgcgc tggcaacgtc aatgatgggg ctgaatgcgc
    14881 tggatattga aacgctgaat aatgacgaac atgcgtggga tgcggtagtc gaagagttcg
    14941 atggttatcg gcaaatctgg cgcaaacgtg gcgttatgcc gatgctgcgg gcgctgatgt
    15001 cggcgcgtaa cattgcagaa aacttgctgg caacggcagg cggtgagcgg cgtcttaccg
    15061 atatcttgca tatcagcgaa ctgcttcaag aagccggaac gcagctggaa agtgaacatg
    15121 cgctggtacg ctggttatcg caacatatcc tcgagccaga cagtaatgcc tccagccaac
    15181 aaatgcgcct cgaaagtgat aaacatctgg tgcagattgt cacgatccat aaatcgaaag
    15241 ggctggaata tccgctggtc tggttgccat ttattaccaa tttccgcgtc caggatcagg
    15301 cgttttatca cgatcgccac tcgtttgagg cagttttgga tcttaatgct gcgccagaaa
    15361 gcgtcgacct cgcggaggtc gaacgtctgg cggaagatct gcgtttgctt tacgtggcgc
    15421 tgacgcgttc ggtttggcat tgcagtctcg gcgttgcacc gctggtgcgc cgtcgtggcg
    15481 ataaaaaagg tgacaccgac gtccaccaaa gtgcgctcgg gcgtttgctg caaaaagggg
    15541 aaccgcaaga tgcggcaggg cttcgcacct gtattgaagc gttatgcgat gatgatattg
    15601 cctggcaaac ggcacaaact ggtgataacc agccctggca ggttaatgat gctttaactg
    15661 cagaactgaa tgcgaggaca ctacaacgat tgcccggcga taactggcgt gtcaccagct
    15721 actccggctt gcagcagcgt ggtcacggta tcgctcagga tctgatgcca cggctggatg
    15781 ttgatgccgc aggcgtggtc agcgtcgttg aagaaccgac gttaacaccg catcagttcc
    15841 cgcgcggtgc gtcaccgggg acattcttgc acagtttgtt tgaagacctc gattttaccc
    15901 agccggttga cccgaactgg gtacaggaaa aactggaact cggcggcttt gaatcgcagt
    15961 gggaaccggt gttgaccgag tggatcacgg ctgtcctcca ggcacctctc aatgaaacgg
    16021 gtgttagcct gagtcagctt tccgaccgcg ataaacaggt ggagatggag ttttacctgc
    16081 cgattagcga accgctcatc gccagccagc ttgatgcact aattcgccag tttgacccgc
    16141 tatccgctgg ctgcccgccg ctggagttca tgcaggtacg cggcatgtta aaaggcttta
    16201 tcgacctggt attccgccac gaagggcgtt attacctgct cgactataaa tccaactggc
    16261 tgggtgaaga cagttcggct tacacccaac aggctatggc agcagcgatg caggcacacc
    16321 gctatgatct gcaatatcag ctttataccc tggcgctgca tcgttatctg cgccatcgca
    16381 ttgctgatta cgactatgat cttcactttg gcggcgttat ctatctgttc ctgcgtggcg
    16441 ttgataaaga acatccgcaa caagggattt acacaacccg acccaacgcc gggttgattg
    16501 ccctgatgga tgagatgttt gccggtatga ccctggagga ggcgtaatga aattgcaaaa
    16561 gcaattactg gaagctgtgg agcacaaaca gctacgcccg ctggatgtgc aatttgccct
    16621 gaccgtggcg ggagatgaac atcctgccgt caccctcgcg gcggcactgt taagtcatga
    16681 tgccggagag ggacacgtct gtttgccgct ttcacgactg gaaaataacg aggcgtcgaa
    16741 tccgctgttg gcgacctgtg tcagtgaaat cggtgagcta caaaattggg aagaatgctt
    16801 gctggcttct caagcggtca gcaggggaga tgaacccacg ccgatgatcc tctgtggcga
    16861 tcgtctttat ttgaatcgca tgtggtgtaa cgagcggacg gtagcgcgct tcttcaatga
    16921 ggtgaatcat accattgagg ttgatgaagc tctactggcg caaaccctgg acaaactttt
    16981 tccagtaagc gatgaaatta actggcaaaa agttgcagcg gcagtggcgc tgacgcggcg
    17041 gatctcggtg atttccggcg gcccaggcac cggtaaaacg accaccgtgg cgaagttgct
    17101 ggcagcgtta attcaaatgg ccgacggcga acgctgccgt atccgtctgg ctgcaccaac
    17161 gggtaaagct gccgcgcgct taaccgaatc tctcggcaag gctttgcgac agttaccgct
    17221 gaccgatgaa caaaagaaac gcattccgga agatgccagc actttgcacc gattgctggg
    17281 cgcgcagccg ggtagccagc gtttacgtca tcatgccggt aacccgctgc atcttgatgt
    17341 gctggtggta gatgaagcgt caatgatcga tctgcctatg atgtcgagac tgatcgacgc
    17401 cttgccagat catgcgcgag tgatctttct cggcgatcgt gatcaactgg cctcggttga
    17461 ggctggggct gtgctgggcg atatctgcgc ttatgccaac gcgggcttta ccgccgagcg
    17521 tgccaggcag ctaagccgcc tgacggggac tcacgttccg gcaggaaccg gcacagaagc
    17581 ggcatctttg cgcgacagtc tctgcctgct gcaaaaaagc tatcgtttcg gcagcgattc
    17641 tggcattggt cagttagctg cggcgatcaa ccgtggtgat aaaacggcag tgaaaaccgt
    17701 ttttcagcag gattttactg atatcgaaaa acgactttta cagagcggcg aagattatat
    17761 tgcgatgctt gaggaagctc ttgcgggtta cggacgttat ctggatctgc tgcaagcgcg
    17821 tgccgagccg gatttaatca ttcaggcgtt caatgagtac cagcttttgt gcgccctgcg
    17881 ggaagggccg tttggcgtgg ctggactgaa tgagcgaatt gaacagttta tgcaacagaa
    17941 gcgcaaaatt catcgtcatc cgcactctcg ttggtacgaa ggccgaccgg tgatgattgc
    18001 ccgtaatgac agcgcgcttg ggttgtttaa tggcgatatc ggtattgcgc tggatcgcgg
    18061 gcaggggacg cgcgtctggt ttgcgatgcc ggacggcaat attaagtctg tgcaaccgag
    18121 tcgcttgcca gagcacgaaa ctacgtgggc gatgacggta cataaatcgc agggatcgga
    18181 gttcgaccat gcggcgttga ttttaccgag ccaacgcacg ccggtagtaa cgcgagagct
    18241 ggtatacacc gcggtgaccc gcgcgcgtcg tcgtctgtcg ctgtatgccg atgagcgtat
    18301 attaagtgcg gcaatcgcca ctcgtactga gcggcgcagt ggtctggcgg cgttgtttag
    18361 ttcgcgggaa taaacgtaat tgccggatgc gacgcacgag tgttacgcct gccgcatccg
    18421 gtgattttca tcgcttaccc taaatccgcc atcaacactt tggatttacg ctggtagttg
    18481 tacaactgct ttttgctctc gggcagtaaa tcaatatcca ccggggtaaa tccacgttcc
    18541 tggaaccagt gaatactgcg cgtggtcagc acaaacaatt tgcttaagcc gctctgcttc
    18601 gcctgagcgg caatgcgttc cagcagaacc tcgccccgtg atgaactgcg gtaatccggg
    18661 tgaactgcca cacaggccat ttccccaatc ttctcttccg ggaacggata gagcgcggcg
    18721 caggcaatag tcgtgttatc gcgctgaata atggtgaatt tgtcgatttc catctccagc
    18781 tgctcgcgag aacggcgcac cagaatacct tgctgctcca gcgggcgaat caactccaga
    18841 ataccgccaa tatcgttgat tgttgcgcga cgaatctgct cggcgctttc catcacaatc
    18901 tgcgtaccga taccgtcgcg tgagaacaac tcttgcaaca gcgcgccatc ttcctgataa
    18961 ctgattaaat gacagcggcg cacgccgctg cggcaggctt tcactgcgcc acgcaaaaag
    19021 cgcaccgtac cggagttgta atcgcctttc tcttcctggg cttctacccg tgcttgcgct
    19081 tcgttaggga aaagttcgga gacaatatca ccgtcgtcat tagtaacgcc ctgggaagag
    19141 caaaaaccaa tcatcttttc agctttcagt ttgatggcta gttgagtggc aatctcttcc
    19201 gaggtcagat taaagctctc gccagtgact gaaacagcga ccggccccat tagcactatt
    19261 gcaccgcttt ccagttgacg atggagcgcg tcttcatcaa tccgccggat acgcccgcta
    19321 tggcagtaat ccacgccgtc atcgacgccc agcggctggg caataataaa attgccacta
    19381 acgacgttga tatgcgcgcc ctgcagcggc gtgttattga gactcattga caggcgagca
    19441 gtaatatcca gttgcaatgt tcccgcagcc tgcttcacca gttccagtgt tttggcgtcg
    19501 gtcacacgta tattcttgtg atacagcggt tcgtggtgat gcgcagccag atttgcgtcg
    19561 atctgcggac gtgcgccata gaccaccacc agacggatgc cgaggctgtg caacaacccg
    19621 atatcattaa cgatactgga gaaattctca tgctcaatgg cttcaccgcc gagcatgatg
    19681 acaaacgttt ttccccggtg ggtattgata tagggaaccg aatggcggaa tccctcgacc
    19741 agctcggttt tacgttcctt taccacggca cacccctttg catgattatt tgaaattatt
    19801 gtatttttat tctgtttttt cgcagggtgc aagtgtaaat tttatgcgag agcgactttt
    19861 ttatcagtaa tgccgtgaat ataaaaagaa tgtttaccgt ttattagatg acagattatg
    19921 cgtctttcgc taaagtttcc ggtcaaatta gtcgtttact tgttacacag cttagatttc
    19981 ttttgctcgg gagaggcatg tcaggatcca acactgcaat cagccgtcgt cgtttactgc
    20041 aaggcgcggg cgctatgtgg ctattgagcg taagtcaggt cagcctggct gcggttagcc
    20101 aggtcgtggc ggtgcgcgtc tggcctgcgt ccagctacac ccgcgtgacg gtagaatcaa
    20161 atcgtcagct gaaatataag cagttcgcgt tgagtaaccc tgaacgcgtg gtggtggata
    20221 tcgaagatgt aaacctgaac tcggtactca aggggatggc ggcacaaatt cgtgcagacg
    20281 acccgttcat caagtcggcg cgcgtcgggc aatttgaccc gcaaaccgta cgcatggttt
    20341 ttgaattaaa gcaaaacgta aaaccgcagc tgtttgccct tgcgccggtc gccgggttta
    20401 aagagcgtct ggtgatggac ctctatccgg ccaatgcaca ggatatgcag gacccgctgc
    20461 tggcgctgct ggaggattac aacaaaggcg acctcgaaaa gcaggtgccg ccagcgcaaa
    20521 gtggtccaca accgggtaaa gcagggcgcg atcgtccgat tgtcattatg cttgaccctg
    20581 gccacggtgg cgaagactcc ggtgcggtgg ggaaatacaa aacgcgcgaa aaagatgtgg
    20641 tattgcaaat agctcgccgt ttgcgctctc tgatcgagga agagggcaat atgaaggtgt
    20701 acatgacgcg caatgaagac atcttcattc cgttgcaagt gcgcgtagca aaagcccaga
    20761 aacagcgtgc tgacctgttt gtctctatcc atgccgacgc ctttaccagt cgtcagccga
    20821 gcggttcctc ggtgtttgcg ctctcaacca aaggcgcaac cagtactgcg gcaaaatatc
    20881 tggcacaaac ccagaacgcc tcggacttga ttggtggcgt gagcaaaagc ggtgaccgct
    20941 atgtcgacca caccatgttc gatatggtgc agtcgctgac tattgctgac agccttaagt
    21001 ttggtaaagc ggtactgaat aagctcggaa aaatcaacaa gctgcataaa aatcaagttg
    21061 aacaggccgg gtttgccgta ctaaaggcac cagatattcc ctccattctg gtcgaaacgg
    21121 cgtttatcag taacgttgag gaagagcgta aactgaaaac ggcgactttc cagcaggaag
    21181 ttgcggagtc tattcttgcg gggattaaag cgtattttgc cgatggggca acgctggcga
    21241 gaaggggatg ataaaaaggc gctgaatggc gcttttttta tgggagatca gaaacaaaaa
    21301 aacacccgtt agggtgttca at
//
LOCUS       DAJTYL010000065        21266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYL010000065 DAJTYL010000000
VERSION     DAJTYL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.065"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            14..499
                     /gene="tnpA"
                     /locus_tag="OO572_002400"
     CDS             14..499
                     /gene="tnpA"
                     /locus_tag="OO572_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6661377.1"
                     /translation="MKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEKLRTYF
                     SNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRPDIAVR
                     YYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFTAHRIK
                     D"
     gene            complement(538..885)
                     /gene="grxD"
                     /locus_tag="OO572_002401"
     CDS             complement(538..885)
                     /gene="grxD"
                     /locus_tag="OO572_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6661378.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1220..2047
                     /gene="mepH"
                     /locus_tag="OO572_002402"
     CDS             1220..2047
                     /gene="mepH"
                     /locus_tag="OO572_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6661379.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2175..2756
                     /gene="sodB"
                     /locus_tag="OO572_002403"
     CDS             2175..2756
                     /gene="sodB"
                     /locus_tag="OO572_002403"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6661380.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2902..4071)
                     /locus_tag="OO572_002404"
     CDS             complement(2902..4071)
                     /locus_tag="OO572_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661381.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4237..4326)
                     /gene="ynhF"
                     /locus_tag="OO572_002405"
     CDS             complement(4237..4326)
                     /gene="ynhF"
                     /locus_tag="OO572_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6661382.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4625..5650
                     /gene="purR"
                     /locus_tag="OO572_002406"
     CDS             4625..5650
                     /gene="purR"
                     /locus_tag="OO572_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6661383.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5647..6579)
                     /locus_tag="OO572_002407"
     CDS             complement(5647..6579)
                     /locus_tag="OO572_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6661384.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6692..7903
                     /locus_tag="OO572_002408"
     CDS             6692..7903
                     /locus_tag="OO572_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6661385.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8194..9342
                     /gene="cfa"
                     /locus_tag="OO572_002409"
     CDS             8194..9342
                     /gene="cfa"
                     /locus_tag="OO572_002409"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6661386.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9382..10023)
                     /gene="ribE"
                     /locus_tag="OO572_002410"
     CDS             complement(9382..10023)
                     /gene="ribE"
                     /locus_tag="OO572_002410"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6661387.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10238..11611
                     /gene="mdtK"
                     /locus_tag="OO572_002411"
     CDS             10238..11611
                     /gene="mdtK"
                     /locus_tag="OO572_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6661388.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11652..12908)
                     /locus_tag="OO572_002412"
     CDS             complement(11652..12908)
                     /locus_tag="OO572_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6661389.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13216..13292
                     /locus_tag="OO572_002413"
     tRNA            13216..13292
                     /locus_tag="OO572_002413"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13250..13252,aa:Val,seq:gac)
     gene            13297..13373
                     /locus_tag="OO572_002414"
     tRNA            13297..13373
                     /locus_tag="OO572_002414"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13331..13333,aa:Val,seq:gac)
     gene            13481..13786
                     /locus_tag="OO572_002415"
     CDS             13481..13786
                     /locus_tag="OO572_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6661390.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13912..15334
                     /locus_tag="OO572_002416"
                     /pseudo
     CDS             13912..15334
                     /locus_tag="OO572_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15346..16158)
                     /locus_tag="OO572_002417"
     CDS             complement(15346..16158)
                     /locus_tag="OO572_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661391.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16162..16947)
                     /locus_tag="OO572_002418"
     CDS             complement(16162..16947)
                     /locus_tag="OO572_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6661392.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16944..17612)
                     /locus_tag="OO572_002419"
     CDS             complement(16944..17612)
                     /locus_tag="OO572_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6661393.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17676..18314)
                     /locus_tag="OO572_002420"
     CDS             complement(17676..18314)
                     /locus_tag="OO572_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6661394.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18327..20428)
                     /locus_tag="OO572_002421"
                     /pseudo
     CDS             complement(18327..20428)
                     /locus_tag="OO572_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20449..21075)
                     /locus_tag="OO572_002422"
     CDS             complement(20449..21075)
                     /locus_tag="OO572_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6661395.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         5291 a         5528 c         4977 g         5470 t
ORIGIN      
        1 gatactttgg tctgtgaaaa aagaaaccga tatccggcgt ggaaggcatt gtgttttcct
       61 gatgcatgtt cacctggtct ttgtcaccaa ataccgacgc cagatttttg accatgacac
      121 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct gattttgcag ctgaactggt
      181 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactatcctc ccaaactggc
      241 gatatccagt ctggtaaaca gcctcaaagg catatcgaat aggttactgc gacgagatcg
      301 accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg gctattttgc
      361 cagtagctgc gaaggtgcgc caatatccgt catccgccag tacattgaac aacagcaaac
      421 acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggacgag gttttacggc
      481 gcaccggata aaggactgac ggatgcccgt tcgcatccgt cagtattgca ggacggatta
      541 ttccgcgtcc ggctcttcag acttgtattt agcggcagtt tctttgatca gctgctgcag
      601 ttcgccacgc tgatacattt cgatcacgat atcacaaccg ccgaccagct cgccgtcaac
      661 ccacagttgc gggaaggtcg gccagttagc atatttcggc agttccgcac gaatgtccgg
      721 attctgcaga atatcaacat aggcaaaacg ttcgccacat gcggcaagcg cctggactgc
      781 ctgggcagag aaaccgcagc tcggcagttt cggtgaacct ttcatgtaca gcaggatcgg
      841 gttttcagca atctggcgtt ggattttttc gatagtggtg ctcattgtct tgcttcctca
      901 aacttcttta cggcagtaat ctgacattgt agcgggtcag tgcggcatcg gaaaataaca
      961 ttttcatcac gcttttgcta ttttatccct ttgctctatt tgttgcattt caaatattcg
     1021 tttcttctct gatgcgcggc aaactggcgt atttataacc atttttagct ttcactgctg
     1081 catttttttg cgctcgccaa cgaaacgtat tttttaacaa taaaagctat taactttctc
     1141 ttcttctatg cattagaatc atcaagtttt gtaaatcaga cgcaggcatg atagacctgc
     1201 cttaacagag ggacgctcag tggcgcggat aaaccgtatt tcgatcacgc tctgtgcttt
     1261 actttttacc accctgcctt taacgcctat ggcccatgct tcaaagcaag ccagggagag
     1321 ttctgctacc actcatatca ccaaaaaagc agataaaaag aaaagcacgg caaccaccaa
     1381 aaagacccag aaagccagcc agaaaacagc gaaaaaagcc gccagtaaaa gtatgaccaa
     1441 aagcaaaacc gcttcttctg ttaaaaaatc ttccattacc gcttctaaaa acgccaaaac
     1501 tcgcagcaaa cacgccgtca ataaaacggc ctcagccagc tttaccgaaa agtgtaccaa
     1561 acgtaagggc tataaatcgc attgtgtgaa agtcaaaaat gccgcgtcag gaacccttgc
     1621 cgacgcgcac aaagcgaagg tgcaaaaagc cacgaaagtg gcaatgaata aactgatgca
     1681 gcaaattggt aagccatatc gttggggcgg cagctcaccg cgtaccggtt ttgattgcag
     1741 cggcctggtt tattacgctt ataaagattt ggtgaaaatt cgtattccgc gtacggcgaa
     1801 tgaaatgtat cacctgcgtg atgcaggacc tatcgaacgt agtgagctga aaaacggcga
     1861 cctggtcttt ttccgtactc agggacgcgg tacagccgat catgtcggcg tgtatgtcgg
     1921 caacggcaaa tttattcagt caccgcgcac aggtcaggaa attcaaatca cttctctcag
     1981 tgaagactac tggcagcgcc actatgttgg cgctcgtcgg gtaatgaccc caaaaacact
     2041 tcgctaaaac tttaccctgt tgttacggca acagggtaag ttcatctttt gtctcacctt
     2101 ttaatttgct accctatcca tacgcacaat aaggctattg tacgtatgca aattaataat
     2161 aaaggagagt agcaatgtca ttcgaattac ctgcactacc atatgctaaa gatgctctgg
     2221 caccgcacat ttctgcggaa actatcgagt atcactacgg caagcatcat cagacttatg
     2281 tcactaacct gaacaacctg attaaaggta ccgcgtttga aggtaaatca ctggaagaga
     2341 ttattcgcag ctctgaaggt ggcgtattca acaacgcagc tcaggtctgg aaccatactt
     2401 tctactggaa ctgcctggca ccgaacgccg gtggcgaacc gactggaaaa gtcgctgaag
     2461 ctatcgccgc atcttttggc agctttgccg atttcaaagc gcagtttact gatgcagcga
     2521 tcaaaaactt tggttctggc tggacctggc tggtgaaaaa cagcgatggc aaactggcta
     2581 tcgtttcaac ctctaacgcg ggtactccgc tgaccaccga tgcgactccg ctgctgaccg
     2641 ttgatgtctg ggaacacgct tattacatcg actatcgcaa tgcacgtcct ggctatctgg
     2701 agcacttctg ggcgctggtg aactgggaat tcgtagcgaa aaatctcgct gcataataac
     2761 tgatggcaaa tgcagcattg cctgatgcgc ttcgcttacc atgccaacgc ggatgaggcg
     2821 ttaacagcgc attcggcaag ataaatcgca cgttgtcagc aactgtaacg cagaaggtta
     2881 tccttctgcg tttttgttta attagctgtt agcaacgcaa actgtttcag gttgttttct
     2941 ggctgacata aacaccagca ataatgccag tcccgcgaca atcgcccccg tcaccggcac
     3001 aaagctgtat cccagcccag cggaaattac cgcaccacca gcagctgctc ccagcgcatt
     3061 tccaagatta aaggcaccaa tattgactga tgaagacaga cctggcgctt cactggcgac
     3121 atgcatcacg cgcatctgta acggaggtac aaccgcaaag gttgctgcgc cccacaccac
     3181 catgctaata gctgcgccga actcattgcg ggccaggaac gggattgcca gcataatcac
     3241 catcagcagc aacaaaaagc ctttcaacgt gccgttaact gaacgatctg ccagtttgcc
     3301 gccgagatag ttaccgatag agaatccgac accaatcagc accagcattg ccgtgacgaa
     3361 caccggtgtt gcgtgggtaa tactttgcag taccggagag atataggtgt agagggtaaa
     3421 cattgcacca gctcccagta ccgtcgtcag caatgcagac agcacctgcg gacgcattaa
     3481 taccgccagc tcttttttca cttcaggtcg tgcccctgta ccacctttag gtaatgagaa
     3541 gaacagactt accattgaaa tcactcccag ccccgccgtt gccagaaatg acatccgcca
     3601 gccgatggtt tcacccaacc aggtcgccgc cggcacgcca ccgatatttg ccagggttaa
     3661 ccccataaac atagtggcaa ctgcgctggc ctgtttatgt tttggcacca cgcttgcggc
     3721 cacgactgaa cccaaaccaa aaaatgctcc gtgattcagg ctggtcaaaa tacgtgaaag
     3781 catcagggtc atataatccg gcgcgatggc ggaaagaacg ttgcctagcg tgaaaattgc
     3841 catcaggaaa atcaacgcac tgcggcgggc acgatgagaa agtagaagcg tcatcagcgg
     3901 cgcgcccacc attacgccaa ctgcataggc actgattaac attccggcag cgggaatcga
     3961 gacatccaca ccgcgcgcaa tgacgggcaa caagcccatt ggcgagaact ccgttgtccc
     4021 gataccaaac gcgccaatcg ccagcgccag caacggatag ttaattttca tgccttatct
     4081 ccacctcttc gcgtcattac gcgatattca ttaaagtggc gaaagcatga cagcaatcac
     4141 aaaaaaatga aaataacaaa aagagaaaac acttttgcca ttttgctaac aaacaggaag
     4201 gagatgcgag ggagaacgcg ctccctcgag aggaaatcag tgcagcgcgg cagtcaaacc
     4261 cacggctacg atcaaaccga ggacgataac cgttgttacc agtgaaaatt taaggtcggt
     4321 gctcatcaag ttttctcctt ttttattacc acacaaaaag tgatattacg catttttaca
     4381 cactgtgatg aaaaaatctc ccgtcattta taatgataag tgtttttacc acttcccctt
     4441 ttcgtcaaga tcggccaaaa ttccacgctt acactatttg cgtactggcc attgacccct
     4501 tcctgacgct ccgtgtcgtt tttccggcgt accgcaacac ttttgttgtg cgtaaggtgt
     4561 gtaaaggcaa acgtttacct tgcgattttg caggagctga agttagggtc tggagtgaaa
     4621 tggaatggca acaataaaag atgtagcgaa acgagcaaac gtttccacta caactgtgtc
     4681 acacgtgatc aacaaaacac gtttcgtcgc tgaagaaacg cgcaacgccg tgtgggcagc
     4741 gattaaagaa ttacactact cccctagcgc ggtggcgcgt agcctgaagg ttaaccacac
     4801 caagtctatc ggtttgctgg cgaccagcag cgaagcggcc tattttgccg agatcattga
     4861 agcagttgaa aaaaattgct tccagaaagg ttacaccctg attctgggta atgcgtggaa
     4921 caatcttgag aaacagcggg cttatctgtc gatgatggcg caaaaacgcg tcgatggtct
     4981 gctggtgatg tgttctgagt acccagagcc gttgctggcg atgctggaag agtatcgcca
     5041 tatcccaatg gtggtgatgg actggggtga agcaaaagct gacttcaccg atgcggtcat
     5101 tgataacgcg ttcgaaggcg gctacatggc cgggcgttat ctgattgaac gcggtcaccg
     5161 cgaaatcggc gtcatccccg gcccgctgga acgtaacacc ggcgcaggcc gccttgccgg
     5221 ttttatgaag gcgatggaag aagcgatgat caaggtgccg gaaagctgga ttgtgcaggg
     5281 tgactttgaa cctgaatccg gttatcgcgc catgcagcaa atcctgtcgc agccgcatcg
     5341 ccctactgcc gtcttctgtg gtggcgatat catggcaatg ggcgcacttt gtgctgctga
     5401 tgaaatgggc ctgcgcgtcc cgcaggatgt ttcgctgatc ggttatgata acgtgcgcaa
     5461 cgcgcgctat tttacgccgg cgctgaccac gatccatcag ccaaaagatt cgctgggtga
     5521 aacagcgttc aacatgctgt tggatcgtat cgtcaacaaa cgtgaagaac cgcagtccat
     5581 tgaagtgcat ccgcgtttga ttgaacgccg ctccgtggct gacggcccgt tccgcgacta
     5641 tcgtcgttaa tcacccgttg cgggagtctc ttccggctcc cgcagccact ccttattcag
     5701 cgtctcacta tcgctgagat actcaagcag ccaggttaac gcaggcgaca tatcattttg
     5761 ctgccatgtc agacaacatg ccgaatccgg gaaggggttt tccagttcaa gtgctaccca
     5821 cttcccctca ttaagccacg gtttggcgaa atgtgttggc accatcccta tgcataatcc
     5881 tgccgagata caggttgccg atgattccca gtcaggtacg acaagtcttt tttggttatc
     5941 cagcaaccag gtaatacgtt taggtagcgt tcgcgaggtg tcttcgcgca ccaacgacgg
     6001 ccagttgcgc aacgtatcat cgctgaacgg gccatccatc aacgccagcg ggtggtgact
     6061 ggcaacaacg caactccagc ttagcatccc catatcccgg aaggcataac gaccgcctac
     6121 cggaatcgcg cgtgttgcgc caattgccag ttccacgcgc ccgtcggaaa gcgcatccca
     6181 gacaccgttg aacacttcct gaaagacaag aagttcgaca tcatcaaaat ggcgataaaa
     6241 atcaacgatc atctgccgtg tacgttctgg cctgacaata ttatccactg cgatagctaa
     6301 ctgaccgcgc cagccgttcg ctatctgctg acattgctgg cgggtgatct gcattttttt
     6361 gacaacagag cgcccttctt tgagaaacca cgctccagca gcggtcagct ccacatcacg
     6421 gtgccgtcgt tcaaagagcg gtaccgccag ccactcttcc agctgacgca cggtatagct
     6481 gaccgcagaa ggaacacgat gcagctcctg tgccgcagcg ctaaaactac cattacgcgc
     6541 taccgcatca acaacttcga gtgaatattc tgaccacata gtctgcctgc aaaatttttg
     6601 aaaccagtca tcaaatatta ccgtttcaca acactaattt cactccctac actttgcggc
     6661 ggtgtttaat tgagagattt agagaatata catgcaacct gggaaaagat ttttagtctg
     6721 gctggcgggt ttgagcgtac tcggttttct ggcaaccgat atgtatctgc ctgctttcgc
     6781 cgccatacag gccgacctgc aaacgcctgc gtctgctgtc agtgccagcc ttagtctgtt
     6841 ccttgccggt tttgccgcag cccagcttct gtgggggcca ctctccgacc gttatggtcg
     6901 taaaccggtg ttattaatcg gcctgacaat ttttgcgtta ggtagtctgg ggatgctgtg
     6961 ggtagaaaac gccgccacgc tgctggtatt gcgttttgta caggctgtgg gggtctgcgc
     7021 cgcggcggtt atctggcaag cattagtgac agattattat ccttcacaga aagtcaaccg
     7081 tatttttgcg accatcatgc cgctggtggg tctgtctccg gcactggctc ccctgttagg
     7141 aagctggctg ctggtccatt tttcctggca ggcgattttc gccaccctgt ttgccattac
     7201 cgtggtgctg attctgccta ttttctggct caaacccacg acgaaggccc gtaacaatag
     7261 tcaggatggt ctgaccttta ccgacctgct acgttctaaa acctatcgcg gcaacgtgct
     7321 gatatacgca gcctgttcag ccagtttttt tgcatggctg accggttcac cgttcatcct
     7381 tagtgaaatg ggctacagcc cggcagttat tggtttaagt tatgtcccgc aaactatcgc
     7441 gtttctgatt ggtggttatg gctgtcgcgc cgcactgcag aaatggcaag gcaagcagtt
     7501 attaccgtgg ttgctggtgc tgtttgctgt cagcgtcatt gcgacctggg ctgcgggctt
     7561 cattagccat gtgtcgctgg tcgaaatcct gatcccattc tgtgtgatgg cgattgccaa
     7621 tggcgcgatc taccctattg ttgtcgccca ggcgctgcgt cccttcccac atgcaaccgg
     7681 tcgcgccgca gcgttgcaga acactctgca actgggtctg tgcttcctcg caagtctggt
     7741 agtttcctgg cttattagta tcagcacgcc attgctcacc accaccagcg tgatgttatc
     7801 aacagtagtg ctggtcgcgc tgggttacat gatgcaacgt tgtgaagaag ttgactgccc
     7861 gaatcatggc aatgccgaag tcgctcatag cgaatcacac tgatctatat cgatatactt
     7921 atacttaggc tgctaacaaa attttgttgt atgattgaaa ttagcggcct atactaattt
     7981 cgagttgtta aagctacgat aaatattatg tttttacggg gacaggatcg ttcccgactc
     8041 actatggatg gtcatttcgg caagggttcc tcctttccct ctgttctacg tcggattata
     8101 gactcgcggt tttttctgcg agatttctca caaagcccaa aaagcgtcta cgctgtttta
     8161 aggttctgat caccgaccag tgatggagaa actatgagtt catcgtgtat agaagaagtc
     8221 agtgtaccgg atgacaactg gtaccgtatc gccaacgaat tacttagccg tgccggtata
     8281 gccattaacg gttctgcccc ggcggatatt cgtgtgaaaa accccgattt ttttaaacgc
     8341 gttctgcagg aaggctcttt ggggttaggc gaaagttata tggatggctg gtgggaatgt
     8401 gaccgactgg atatgttttt tagcaaagtc ttacgcgcag gtctcgagaa ccaactcccc
     8461 catcatttca aagacacgct gcgtattgcc ggcgctcgtc tcttcaatct gcagagtaaa
     8521 aaacgtgcct ggatagtcgg caaggagcat tacgatttgg gtaatgactt gttcagccgc
     8581 atgcttgatc ccttcatgca atattcctgc gcttactgga aagatgccga taatctggaa
     8641 tctgcccagc aggcgaagct caaaatgatt tgtgaaaaat tgcagttaaa accagggatg
     8701 cgcgtactgg atattggctg cggctggggc ggactagcgc actacatggc atctaactat
     8761 gacgtaagcg tggtcggcgt caccatttct gccgaacagc aaaaaatggc tcaggaacgc
     8821 tgtaaaggcc tggatgtcac cattttgctg caagattatc gtgacctgaa cgaccagttt
     8881 gatcgtattg tttctgtggg gatgttcgag cacgtcggac cgaaaaatta cgatacctat
     8941 tttgcggtgg tggatcgtaa tttgaaaccg gaaggtatat tcctgctcca tactatcggt
     9001 tcgaaaaaaa ccgatctgaa tgttgatccc tggattaata aatatatttt tccgaacggt
     9061 tgcctgccct ctgtacgcca gattgctcaa tccagcgaac cccactttgt gatggaagac
     9121 tggcataact tcggtgctga ttacgatact acgttgatgg cgtggtatga acgattcctc
     9181 gccgcatggc cagaaattgc ggataactac agtgaacgct ttaaacggat gtttacctat
     9241 tatctgaatg cctgcgcagg tgctttccgc gcccgtgata ttcagctctg gcaggtggtg
     9301 ttctcacgcg gtgttgaaaa cggccttcga gtcgctcgct aaaggctatt ctatcgcccc
     9361 ctcttcgggg gcgagttcag atcaggcttc tgtacctggt tgattcatgg cattttctcg
     9421 tgccgccagc acacgttcta ccgtatctac cactgcctga gtttgtggat cgatttcaat
     9481 gttgacgcgt gcgccaagtt ttttcttccc aagagtcgtg cgttccagtg tttccggaat
     9541 taaatggacg caaaaacgcg ttggcgtgac ttcgccgacg gtcaggctaa taccgtcgat
     9601 gccaataaat cctttgtaca ggatatattt catcaactga ctatcctgga ctttaaacca
     9661 gatctggcga ttattttccg aggttaatat tttcgccact tcagcggtgg tcataatatg
     9721 acctgacatt aagtgtccgc caatttcatc actgaatttc gccgcacgct caacgtttac
     9781 ccaatccccc acttttaaat cgccaagatt ggtaatgcgt aacgtttctt tcatcaggtc
     9841 aaaactgaca tggttgccgt taatttccgt cacggtcagg cagcaaccgt tatgcgccac
     9901 ggaagcaccg gtttccaggc cgtccagcat gtggtcgggt aactccacca catgcgtacg
     9961 aaaatttggt ttctcgtcaa tcgacaccag ttttgcggtg ccctgtacaa tccccgtaaa
    10021 catacttaca actcctgaaa tcagttaaga cattctgttc agcacaatag caggtggaaa
    10081 acgcccttac cagtgaaggg gtaagaatgg ctattttttc actggagaat taataaatcc
    10141 tcgctacaat agactgaatt tcccctgctt cttctttttg ctgcccattc aggcggcttt
    10201 ttagtctctc atataactac aaataaaagg tgttcacgtg cagaagtata tcagtgaagc
    10261 gcgtctgtta ttagcattag caatcccggt gattctcgcg caaatcgccc aaactgcgat
    10321 gggttttgtc gataccgtga tggcgggcgg ctatagtgcc accgacatgg cggcggtcgc
    10381 tatcggtact tctatctggc ttccggcgat cctctttggt cacggactgc tgctggcatt
    10441 aacgccggtt atcgcgcaat taaatggttc cggtcgacgt gagcgcattg cgcatcaggt
    10501 gcgacaaggt ttctggctgg caggttttgt ttccgttctc attatgctgg tgctgtggaa
    10561 tgcaggttac attatccgct acatggaaaa catcgatccg gctctggcgg acaaagccgt
    10621 gggttatctg cgtgcgttgt tgtggggcgc gccgggatat ctgttcttcc aggttgcccg
    10681 taaccaatgt gaaggtctgg caaaaaccaa gccgggtatg gtaatgggct ttatcggcct
    10741 gctggtgaac atcccggtga actatatctt tatttatggt catttcggta tgcctgagct
    10801 cggtggcgtt ggttgtggcg tggctactgc ggcggtgtat tgggtcatgt tccttgccat
    10861 ggtttcttac attaaacgcg cccgctccat gcgcgatatt cgtaacgaaa aaggcaccgc
    10921 taagcccgag cctgcggtta tgaaacgact gattcaactc ggtttgccga ttgcgctggc
    10981 actgttcttt gaagtgacac tgtttgccgt cgtggctctg ttagtgtctc cgctcggtat
    11041 tgttgatgtc gcaggacacc agattgccct gaactttagt tcactgatgt ttgtgcttcc
    11101 aatgtcgctg gcggcagcgg taactatccg cgtaggttat cgtctgggtc agggctcaac
    11161 gctggatgcg caaaccgctg cgcggaccgg gcttatggtg ggtgtctgta tggcaaccct
    11221 gacggccatt ttcacggttt cactgcggga gcaaatcgcc ctgttgtaca acgacaatcc
    11281 cgaggttgta acgctggctg cgcatttgat gttgctggcg gcggtatatc agatttctga
    11341 ctcaatccag gtgattggca gtgggatttt gcgtggttat aaagatacgc gttccatttt
    11401 ctatatcacc tttacagctt actgggtgct gggcttgcca agcggctata ttctggcact
    11461 gaccgatctg gtcgttgaac ctatggggcc agcaggcttc tggataggct ttattatcgg
    11521 cctgacatcg gcagccatta tgatgatgct gcgtatgcgg ttcctgcaac gtatgccgtc
    11581 agccatcatt ctgcaacgag cgtcccgcta ataaagacaa ggcgcaacct tcacgggttg
    11641 cgcctgtgtt tttacgcagg ctggagcgtt gcgccaatcc cgtcttcgtc tggctgtaat
    11701 ttcagagcgt tacacagagt ttgccgactg gcatctgtta tctcaacgcg tatatccccg
    11761 ttaacttccc ctttcacatt gatgaagtca ggtgaaattt ctttcgcact ggtttgcagg
    11821 caaatcgctc cctctccgcg caggcgtttc actttcagtt gcacatgcgt tgccgcacca
    11881 gtgatcagcc acagttttcc gccattcaga ttgatgttac caacaatgaa agtgacgctg
    11941 tcacctttta ccgtactgcc gtcgagaatc aacacaccgc cctgctgtac agtaatcgtt
    12001 gattgttgca gctctgcgcc ataaccaata atcattgaag cgccgtccag taatgcaaca
    12061 ctcccggtca gttcaaccgg tgcgcgatct tcctctacga caaaattttc gtccgcgctg
    12121 gtgggtgaca acaggatcac tgttccacct tgtattaccg cattttccag cgtaccggca
    12181 tgcacttcag cccgcccccc cacattcacc gacaggtttt gcgtcttacc ggaactgtaa
    12241 agctgaaggc catccgtccc cagacgataa atggatccat tctcaacaat agtgtcagag
    12301 tcttcaccac cgagattatt catggtgcca ccagcaagca acgtatcact ggctttcccg
    12361 gccagcataa aaacgccgcc ctgattaatc tcacaattgc tggcttctcc acttggatac
    12421 accaacaatt caccaccttc atctaccacg accgctgagg ttgtcccatt aaccaacagg
    12481 ccgccctctt gatcgagaat gattttttcc gcgcgatgtc cttccagtac acgcaggtta
    12541 ccgccatttt ccagcaacaa accgcaggca taaccctgat caacgctgaa ttcaccttcg
    12601 ggatggcggc cattaaccgt agcgagcgta gagagactaa ttgcgcctcc ctcggactga
    12661 acaatatctg tggcaatggc accgtcgctg accgatagcg tgccattgtt accgtactgt
    12721 gtgcatttgg cgatcccacc ggttgccact ttcaaccagc cagcccccgt gaccacggta
    12781 ttttcggctc ttccgccagc ttcaacaatc aactcgcctt cagtaagttg cgtcgcgcca
    12841 aggacctcgc cggtcttaac aatttgcaca ttcccgtcgc tcatcaagtt tttcgcatca
    12901 gatcccatga tttattcctt tgctgcatct gtgtgccttt attgctacct aagtgtaaag
    12961 gctacggagg atttatccac gacagatttg agatggtggc aaacaactct gtttaaactc
    13021 tgatacacga attattgggt tgtatcagat gtaaatgcga tcctgaataa aaatcgccct
    13081 tgcaaatcaa caaaatatag ccaatttgct taaaagctct gcaaacggtc gaaatagcgc
    13141 agaaaattac gttttgcctc ttgccacctt tccactctgc cgctaatatt cgtccccgtt
    13201 gtcacctaca acgttgcgtt catagctcag ttggttagag caccaccttg acatggtggg
    13261 ggtcgttggt tcgagtccaa ttgaacgcac catcctgcgt ccgtagctca gttggttaga
    13321 gcaccacctt gacatggtgg gggtcggtgg ttcgagtcca ctcggacgca ccagattttc
    13381 ttaatctggt cttttccttt ttccctctgt ttcttctctg tatccaatac gttaaaagat
    13441 ttacactgtc ttcgtatgcg ttatcagaag gagaatcgct atggcaactt tgttacaact
    13501 tcattttgct tttaatggcc catttggcga cgcaatggct gagcagctta aaccacttgc
    13561 tgagtcgatt aatcaggaac ctggttttct gtggaaggta tggacagaaa gtgaaaagaa
    13621 ccacgaagcc ggtgggatct accttttcac tgatgaaaaa agcgcccttg cctatctgga
    13681 aaaacatact gcccgactga aaaatctcgg cgttgaggaa gttgtcgcca aagttttcga
    13741 tgtcaatgag ccacttagtc aaatcaatca ggcaaaactc gcctgacaga atttaatcaa
    13801 gggcggttag cgcccttttc atccctgtct gaaatttctc aaattctaaa aatctcaacc
    13861 aaacttatct gataacacta aattcgaaag aatgcgtaca ggtaagtaac aatgaaaaaa
    13921 attgctattg tgggtgccgg gcctacgggg atctacacct tattctcgct tctacagcaa
    13981 caaactccac tttctatttc tatcttcgag caggctgacg aggccggtgt cgggatgcca
    14041 tacagtgatg aggaaaactc aaaaatgatg ctggcaaata ttgccagtat tgaaataccg
    14101 ccgattaatt gtacgtatct cgaatggcta caaaagcaag aagccagcca tctccagcgt
    14161 tatggcgtta aaaaagaaac cttgcacgat cgtcagtttt taccgcgaat tctgctgggc
    14221 gaatatttcc gcgatcaatt tttacgatta gtagaccagg cacgacagca aaaatttgca
    14281 gtggctgttt atgaatcatg ccaggttacc gatctgcaaa ttacaaatgc tggcgtcatg
    14341 ctcgctacaa atcaggattt acccagcgag acgtttgatt tagcggtgat tgccacgggt
    14401 cacgtctggc ctgatgaaga agaagcaacc agaacgtatt ttcccagccc gtggtcaggc
    14461 ctgatggaag caaaggtcga tgcgtgtaac gtgggtatta tgggaacatc cttgagcgga
    14521 ctggatgcgg caatggcagt ggctattcag catggttcgt tcattgaaga tgataaacaa
    14581 cacgtcgttt ttcaccgcga taacgcaagt gaaaagctaa atattacgtt gatgtcgcgc
    14641 acgggtattt tacccgaagc cgatttctat tgccctattc cctacgagcc cttacacatc
    14701 gtcaccgatc aggcattaaa tgctgagatt caaaaaggtg aagagggcct tttggatcgg
    14761 gtatttagat tgatagtaga ggaaatcaag tttgctgatc cagactggag ccaacgcata
    14821 gccttagaga gcctgaatgt cgattccttt gctcaagcct ggtttgccga gcgcaaacaa
    14881 cgcgacccat ttgactgggc agaaaaaaat ctccaggaag tcgaacgcaa taaacgagaa
    14941 aaacatactg ttccctggcg ttatgtcatt ctgcgcctgc atgaagccgt acaggaaatt
    15001 gttcctcatc tgaatgaaca cgaccataaa cggttcagta aaggccttgc cagggttttc
    15061 atcgataatt atgcggcaat cccttcagag tctattcgtc gactactggc cttacgtgaa
    15121 gcggggatct gaaatccctg atgttggcga agattatacg ttacagcaac ccgaagatat
    15181 tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc ctttcgttca
    15241 gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg taaagcctgc
    15301 atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc actcaacaaa
    15361 ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt caccttcaat
    15421 ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc ttccccctag
    15481 cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt caccgctggc
    15541 aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa agctggcgat
    15601 ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac aaacgcgtac
    15661 tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc gcgaagaatt
    15721 accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt taaagccgac
    15781 acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc cagcggttgc
    15841 gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc gggcaaactg
    15901 actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg aaacttcctg
    15961 atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca gactctccgc
    16021 ccagtccgta tatccttcct caatcagtgc atgacgaata tcggcataac taccgccgcg
    16081 tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt ctcgcaggtc
    16141 tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc ttttaaaggt
    16201 ttcgtgtggc gtacgccccg tggtgcaaag ataaagatga ccgaagataa aaaagaggct
    16261 tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag gaaacacatc
    16321 tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg gcagcaatcc
    16381 atacatgaca ccaacatagg cgacctgctg taagggatta aatttagact gtgttgttgc
    16441 cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgcg tttgttttgc
    16501 cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat ccccaacggc
    16561 attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa cttcatgcac
    16621 cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta tcagcccact
    16681 tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga ccgctttgct
    16741 gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac gagcacgaag
    16801 caaggcgtgc agccccagca ccaaccacat cccaaccagc aatactccgg caatgatcaa
    16861 ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact ggctctgaaa
    16921 ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg ttgaccgaac
    16981 cggcgataca gatgcggttt tccggcacca ggtagctgat attgatagta tttattatcc
    17041 tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc gtgttccgga
    17101 caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc gcagaaatca
    17161 catttatctg ccactttagt gacagggtta aggtagcgaa ccaggtacgg acaagcgcca
    17221 atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc ctgctcatca
    17281 cgccacgatg cccccgtcgg gcatacgtcg atgcacggag catcttcgca gtgctgacat
    17341 gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg aatgtgtgct
    17401 atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc gcgggcgcaa
    17461 atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac ttccttttct
    17521 tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac tgttcccatc
    17581 cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc agtggctgca
    17641 actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa ctgctctacc
    17701 tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc gctaaacaag
    17761 tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg tgcaacatcc
    17821 atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc ttgcatcagc
    17881 atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc gtgtgcaatg
    17941 gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt ttcactcatg
    18001 gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat atccgcgtag
    18061 tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc aaccgaatag
    18121 ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg ctggcgaatg
    18181 aactcagcca ggatttcagc aggttctggt tcggttaccg tatcaacggg gtgacgcatc
    18241 ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa cggcaattca
    18301 tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc tgccagatcg
    18361 gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca accaagctgt
    18421 gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc gtcacgatcc
    18481 attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa aacccaggaa
    18541 cagataagat cgtgttcatt acgcatatct ttggtttgca tcggttttac cgtgtaggca
    18601 cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt catctcttcg
    18661 ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta attacggctt
    18721 ttcagcggcg aaacggtcat tggccagacc cagttgcaca gagtgacggc gttatgcaaa
    18781 cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat tggcgtgtag
    18841 tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac ttcacgttgc
    18901 agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat gcagtcacgg
    18961 ttgaacatac acttaacaat ggaaccgacc tgcgctgacg cttcattggc atggtgaacc
    19021 ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc tttacccaaa
    19081 ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc acccacacga
    19141 tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc cagttgatcc
    19201 cagcgaattt ctgcatactc ttcagctggc agaacacgct tgaacacacc tttgctgtaa
    19261 caataagtaa agtcgcgatg caactgcccg tagttacacc ataggccgta gtcatcgaac
    19321 aaattcagac caaccaggtt accaatcaca cgaccatcgt ctttatcttc aaaatctttc
    19381 ggcccgttcg ggaagatggt ggtatggaca aagtttgcta cacaagtgtt accacctgtg
    19441 ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca acggatcggg
    19501 caagagtggc agccgctcat ttttactgtg tatttctctg ccgccggtcc taagtcaaaa
    19561 acggatttat aggtacgaaa gccgaccgta ttctgattgc ctggcggaat ttcacccgtt
    19621 tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt ccagcgtgac
    19681 ttgggatctg aatactctgc ccacgattgt ggcgtacttg gcacgacatg gttgttattc
    19741 gcaccaataa gttcagtcat catgtaatca ttgagacgct tcatctcctg acgatcggca
    19801 atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga acccattatt
    19861 gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga gagaggaata
    19921 aggttttccc cagcctgacc aatagccgcc acacaggttt ccgggctggt caatcgacaa
    19981 atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt ttccaggcta
    20041 actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc gataataatg
    20101 acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg ggcatcgcga
    20161 ctaaatttcc tttggtaaaa gtagaaagtg aggtgatatt tacgcgagaa ctacaggggg
    20221 caccagatcc aggtaatggg ccggtagcaa agactaattt attcgcttca tcgaaaggtt
    20281 tcgtgcctag cggtacttcg tcatacataa ttttgtagcc gaagcccatg ccaccgacaa
    20341 aacttttaaa cttactggaa tcttcgaggg taatatttcc tgtcgtgaga ttgactctta
    20401 atatattacc tgtccaaccg ttagccatga ttttttcctt tgcaagattc acacagtaat
    20461 atctttccac tcgataattt ttaacgcccc tgttgggcag gcgtttgcgc attcaccgca
    20521 taacacacat ttcgaggatt ttttactttc ggtatttacg gtggccatca tccacgggca
    20581 tgccgtagta caggcgctac agccaataca acgtttatga tcgacggtaa tacagccttc
    20641 tttctgctgc caggtaatcg caccaatcgg gcagacgttc atgcattgcg gctctttgca
    20701 ttgacggcag gtgtccgcgg tatagttgag atcgccatac aggccgccgc cagagccaac
    20761 cccgttgtcg ccaaagaaat aattgcgatg gattttgata cgggagaaga atgtccctac
    20821 tgagccatcg ttgaagttgg tacaagagat ttcacagcgg tggcaacccg tgcatcgcgc
    20881 tcgctgggtc accagcaccc ctttgggtgt atttatcaac cctactgtgc cgctatcaat
    20941 atcttcctgt ttgcaaccca aaagtgacag taacgcaggg gcaattgtta gaccagcaag
    21001 gccttttcct gatatacgca agaattctaa tcgcgttaag ccaatatcta atagtggacg
    21061 atcaaccggg ttcatttatt tcgcatcctc ttcgcaaaca cagtgctgac aattcagcag
    21121 cctgatatta agcattcaac taatacgctg ctgactctgt ccccaacacc agatattttc
    21181 cctaacccga tagtgtatga gaaaagattt aacgccgata aactaatcct ttatggttag
    21241 gagaaaaaat aatgtgatta ttctga
//
LOCUS       DAJTYL010000066        21191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYL010000066 DAJTYL010000000
VERSION     DAJTYL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.066"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO572_002423"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO572_002423"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6661396.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO572_002424"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO572_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6661397.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO572_002425"
     CDS             complement(2649..3200)
                     /locus_tag="OO572_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6661398.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO572_002426"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO572_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6661399.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO572_002427"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO572_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO572_002428"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO572_002428"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6661400.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO572_002429"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO572_002429"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6661401.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO572_002430"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO572_002430"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6661402.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO572_002431"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO572_002431"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6661403.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO572_002432"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO572_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6661404.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO572_002433"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO572_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6661405.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO572_002434"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO572_002434"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6661406.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO572_002435"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO572_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6661407.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO572_002436"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO572_002436"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6661408.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO572_002437"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO572_002437"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6661409.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO572_002438"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO572_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6661410.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO572_002439"
     CDS             complement(18170..18565)
                     /locus_tag="OO572_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6661411.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO572_002440"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO572_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6661412.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO572_002441"
     CDS             19481..20074
                     /locus_tag="OO572_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6661413.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO572_002442"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO572_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6661414.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21191
                     /locus_tag="OO572_002443"
     CDS             20794..>21191
                     /locus_tag="OO572_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6661415.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIR"
BASE COUNT         5074 a         5281 c         5604 g         5232 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg a
//
LOCUS       DAJTYL010000067        20973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYL010000067 DAJTYL010000000
VERSION     DAJTYL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.067"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..509
                     /locus_tag="OO572_002444"
     CDS             <1..509
                     /locus_tag="OO572_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6661416.1"
                     /translation="GYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQ
                     LTSQHQYPANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCIS
                     AIHLLDLFLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGS
                     SGGASGRW"
     gene            complement(569..1867)
                     /gene="eno"
                     /locus_tag="OO572_002445"
     CDS             complement(569..1867)
                     /gene="eno"
                     /locus_tag="OO572_002445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6661417.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1955..3592)
                     /gene="pyrG"
                     /locus_tag="OO572_002446"
     CDS             complement(1955..3592)
                     /gene="pyrG"
                     /locus_tag="OO572_002446"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6661418.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3820..4611)
                     /gene="mazG"
                     /locus_tag="OO572_002447"
     CDS             complement(3820..4611)
                     /gene="mazG"
                     /locus_tag="OO572_002447"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6661419.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4707..6941)
                     /locus_tag="OO572_002448"
     CDS             complement(4707..6941)
                     /locus_tag="OO572_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6661420.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6989..8290)
                     /gene="rlmD"
                     /locus_tag="OO572_002449"
     CDS             complement(6989..8290)
                     /gene="rlmD"
                     /locus_tag="OO572_002449"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6661421.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8347..11103
                     /gene="barA"
                     /locus_tag="OO572_002450"
     CDS             8347..11103
                     /gene="barA"
                     /locus_tag="OO572_002450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6661422.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11335..12675)
                     /gene="gudD"
                     /locus_tag="OO572_002451"
     CDS             complement(11335..12675)
                     /gene="gudD"
                     /locus_tag="OO572_002451"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6661423.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12696..14036)
                     /locus_tag="OO572_002452"
     CDS             complement(12696..14036)
                     /locus_tag="OO572_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6661424.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14038..15390)
                     /gene="gudP"
                     /locus_tag="OO572_002453"
     CDS             complement(14038..15390)
                     /gene="gudP"
                     /locus_tag="OO572_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6661425.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15824..16273)
                     /locus_tag="OO572_002454"
     CDS             complement(15824..16273)
                     /locus_tag="OO572_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6661426.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16291..17073)
                     /gene="truC"
                     /locus_tag="OO572_002455"
     CDS             complement(16291..17073)
                     /gene="truC"
                     /locus_tag="OO572_002455"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6661427.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17073..17402)
                     /locus_tag="OO572_002456"
     CDS             complement(17073..17402)
                     /locus_tag="OO572_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6661428.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18024..18569)
                     /gene="syd"
                     /locus_tag="OO572_002457"
     CDS             complement(18024..18569)
                     /gene="syd"
                     /locus_tag="OO572_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6661429.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18637..19485
                     /gene="queF"
                     /locus_tag="OO572_002458"
     CDS             18637..19485
                     /gene="queF"
                     /locus_tag="OO572_002458"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6661430.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19597..20961
                     /gene="ppnN"
                     /locus_tag="OO572_002459"
                     /gene_synonym="ygdH"
     CDS             19597..20961
                     /gene="ppnN"
                     /locus_tag="OO572_002459"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6661431.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5133 a         5614 c         5012 g         5214 t
ORIGIN      
        1 taggttatgg gctggaagaa aaggtaaccg atgctctggc tggagatatc atccgtagca
       61 acatgatacc cgcatttaaa caacaaaaat tagctaaggg attagagtta gctataatcg
      121 ctttgaataa ccaactcact tcacaacacc aatatccggc taatccttca gaaagtgaat
      181 caacgtcttc cactgatcat tattactttg ctattttttg ggtatttgca gtgatgttct
      241 tccctttctg gttttttcat caaggcagta atttttgtcg cgcatgtaaa agtggagtct
      301 gtatttcagc aatccatctt ttagatttat tcctgttctc cgataaaact ttttccattg
      361 ctgtattttc cttctttttt actttcacca tatttatggt ctttacctgt ttatgtgtac
      421 ttcagaaaag agcatctggt agaagttatc attcagacaa cagcggttcc gctggaggct
      481 caagtggcgg cgcatccggc cgctggtaac tccaccataa aaaatgccag cccgaaggct
      541 ggcattatca aatcagataa agtcagactt atgcctggcc tttgatctct ttacgaccgt
      601 tgtacggtgc tttttcgccc agagcttctt cgatacgaat cagctggttg tatttagcaa
      661 cacggtcaga acggctcata gaaccagttt tgatctggcc tgcagcagta ccaacagcca
      721 gatcagcgat ggtagcatct tcagtttcac cagaacggtg agagataact gcagtgtagc
      781 cagcatcttt cgccatcttg attgcagcca gagtttcggt cagagaaccg atctggttga
      841 atttgatcag gatggagtta gcgatacctt tttcgatacc ttctttcagg atcttggtgt
      901 tggttacgaa caggtcgtca ccaaccagct ggattttgtc gcccagaact ttggtctggt
      961 atgcgaaacc gtcccagtca gattcgtcca gaccgtcttc gatagaaacg atcgggtact
     1021 gtttggtcag ttcttccagg aagtgagtga attcttcaga ggtgaacgct ttgttgcctt
     1081 cgccagccag aacgtattta ccatctttgt agaattcaga agctgcgcag tccatcgcca
     1141 gagtgatgtc tttgcccagt tcataaccag cagctttaac agcttcagcg ataacagcca
     1201 gagcttcagc gttggaaccc aggttcggcg catagccacc ttcgtcacca acagcagtgt
     1261 tcatgccttt cgctttcaga acttttgcca ggtgatggaa aacttcagaa cccatgcgga
     1321 tggcttcttt cacagttttc gcgccaaccg gctgaatcat gaattcctgg atatcaacgt
     1381 tgttgtcagc gtgctcacca ccgttgatga tgttcatcat cggaaccggc atggagtatt
     1441 tgcccggagt gccgttcagt tcagcgatgt gctcgtacag cggcatacct ttagcagctg
     1501 cagcagcttt ggcgttagcc agagatacag ccaggattgc gttcgcgccg aatttggatt
     1561 tgttttcggt gccgtccagg tcgatcatga tcttgtcaat gccagcctga tctttagcat
     1621 ctttgccaat cagcgcctga gcgatcgggc cgtttaccgc agcaacagct ttggttacgc
     1681 ctttacccag gaaacgggat ttgtcgccat cgcgcagttc cagagcttca cgggaaccag
     1741 tagaagcacc tgacggagca gctgccatac cgacgaaacc accctccaga tgtacttcgg
     1801 cttcaacagt cgggttacca cgggagtcga tgatttcacg accgatgatt tttacgattt
     1861 tggacattag gttttcctca agtcactagt taaactgaaa ctccagacaa acaacgcgta
     1921 cccaaggtac gcgttgccgt tctaactttt ttacttactt cgcctgacgt ttctggaact
     1981 cgctggcggc tttcacaaag cctgcaaaca gcgggtgacc atcacgtgga gtagaagtaa
     2041 actccggatg gaactggcaa gccacgaacc acgggtgatt cggaacttcg atgatctcga
     2101 ccaactgatc atccccggaa cggcccgcaa cgcgcagacc tgcatcttca atctgtttca
     2161 acagcatgtt gttgacttcg taacggtgac gatgacgctc aacaattgtc ggcgcattgt
     2221 atagctggcg aaccaggcta tcgtcaacca actggcactg ctgtgcgccg agacgcatgg
     2281 taccgccgag atcgctcttc tcgctacgaa cttcaacgtt gccgttttca tcgcgccact
     2341 cggtaatcag cgccacaacc gggtacttac agtctggcac aaattctgta gagttggcgt
     2401 tctccatgtt ggcaacatgg cgagcgtaat caattaacgc cacctgcata cccaggcaaa
     2461 tgcccagata aggaatattg ttctcacgcg caaaacgcgc ggtcgtaatc atgccttcta
     2521 cgccacgata gccgaaaccg ccaggtacga ggattgcgtc cagaccttta aggatttcaa
     2581 cgccgcgcgt ttcaacatct tgtgaatcga tcagtttgat gttgacgctg acacgattct
     2641 tcagcccacc gtgtttcagt gcttcgatca ctgatttata agcatccggc agttcaatgt
     2701 acttgccgac cataccgatg gtgacttcac ttaccggatt cgcttcttcg aagataacct
     2761 gttcccattc ggacagattt gcttccgggc agtttaagct gaatcgttta caaatataat
     2821 cgtccagccc ctgagatttc aacaggcccg gaattttata gatggaatcg acgtctttca
     2881 gagaaataac cgctttttcc ggaacattac agaacaatgc aatcttcgca cgttcgttcg
     2941 ccggtacagc gcgatctgaa cgacaaatca ggatgtcagg ctggataccg atggagagca
     3001 gctcttttac agagtgctga gtcggtttgg ttttgacttc accagacgct gccatgtacg
     3061 gcaccagcgt caggtgcata aacagagtgt gctcacggcc aatttcaaca gccatctggc
     3121 gaatagcttc gaggaacggc aaggattcga tatcacctac tgtaccgccg atttctacca
     3181 gtactacgtc atgaccttcg ccaccttcca gcacgcgctc tttgattgcg ttagtgatgt
     3241 gcggaataac ctgcacggtt gcgccgaggt agtcaccgcg gcgttcttta cgcagaacgt
     3301 cagagtagat acgacccgtg gtgaagttgt tgcggcggct cattttggta cgaatgaaac
     3361 gctcgtagtg ccccaggtcc aagtcggttt cagcgccgtc ttcagtaacg aacacttccc
     3421 cgtgttggat tgggctcata gtacctggat cgacgttgat gtaaggatcc agtttcatga
     3481 tggtcacatt gaggccacgg gcttcaagaa tggctgcgag ggaggctgcg gcaatgcctt
     3541 tacccagaga ggatacgacc ccgccggtca caaaaatata gttcgttgtc atgctgaacc
     3601 tgagaagtta ggttgaaaag acgatggaat aaccaagacg ggaaagcagt atacccgaac
     3661 atgacctgtg ccacaaactt tcattatccc tcctcttcgc cagcgcacta ttgaaatcag
     3721 gagtgagaaa atagcccctt tggggtaaat gtttttgacg caaatcaagc gcttgtcatt
     3781 taaaaaatga cacaaatggc gcttgaccgc gtaattccct tagagatcaa tttcctgccg
     3841 ttttacctgt tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc
     3901 acgcgcggca acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcacttt
     3961 ttgcaatgcg atttctgctt tcgtccctaa atggcgagcc agattaaccg tggcaaacag
     4021 caggtccccc atttcctcct ccagtttagc ctggtcgaca acagcctgcc gcgcctcgta
     4081 catcacctcg tcgatctctt cgtagacttt atcaaccacc gggccaagcg tcgtccagtc
     4141 gaagccaacg ttggcgcaac gtttctggat tttttgcgcc cgcattaaag ccggtaaact
     4201 acgaggaata tcgtccagcg ccgaatgctg cgctttctgc gcgcgctctt cggttttgat
     4261 ttgctcccaa cgggcaagca cttcactact gttttcggca gaactatcag caaaaacatg
     4321 cggatggcga cgctctaatt tatcgctaat agcagcgcaa atatcattaa agtcaaagcg
     4381 cccttcttcc tgagccattt gcgcgtaaaa caccacctgg aatagcagat cgcccagttc
     4441 accgcgaaga tcgtcaaaat cttcacgggc gatggcgtcc agcacttcgt aggtttcttc
     4501 aagggtgtaa ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc
     4561 cggatcgcgc aggcgctgca taatagtgag caaacggtcg atttgattca ttgaattgtc
     4621 ctgaaaattg cgggtctgtc aggtgtaata ccttcgagta aaagatatcg ctggatacgg
     4681 ctgacgccat atccagctat gtaagactaa ctcccgtgca accgacgcgc gtcgataaca
     4741 tccggcacct ggttgagttt acccagcacg cgccccagca cttgcaggtt gtaaatctca
     4801 atggtcatgt cgatggtcgc cagttgctgt ttggtgtcgc tacggctggc aacgccaagc
     4861 acgttcacct tctcgttggc gagaatggtc gtgatatcac gtaacaaccc actacgatca
     4921 ttagccacca cgcggaccac cagcgaatat ccggcggagt agctctcacc ccataccgcg
     4981 tcaacaatgc gttctggcgc atgggagcgc agttccgcca gttgttcgca atcggcgcgg
     5041 tgtactgaaa taccgcgtcc ctgggtaatg aagccgacaa tctcatctcc aggaatcggc
     5101 tggcagcagc gcgcgatgtg gtgcatcagg ttaccaacac cttcaaccac cacacgaccg
     5161 ttatctttac tgcggttttg cggtgtgtag cttttttgct gaagctgttt cagcgcggcg
     5221 gcgtcctgct cttcggcact cggcttatta aattgcgatt gcaggaagtt caccatctga
     5281 ttgagacgga tatccccgcc accaatcgcc gccagcaact cgtcgacatc attgaagttg
     5341 taacgcggca gcagatgttt ttctgcttct ttcaggctga tccccagatg ttccagctcg
     5401 tcgtcaagga tttgccgccc agccagaatg tttttgtcac ggtcctgttt acggaaccag
     5461 gcgtgaattt tcgaacgccc acggctggtt gtgacgtaac cgaggtttgg gtttaaccag
     5521 tcacggctgg ggttcggctg tttctgggtg ataatttcaa tctggtcgcc catttgcagc
     5581 tggtaggtga acggcacaat gcgcccgcca atttttgccc cgatgcagcg gtgtccgaca
     5641 tcactgtgga tgtggtaagc gaagtccagc ggcgttgatc ccgcaggcaa atcaacgaca
     5701 tcacctttcg gcgtaaagac atacacccgg tcgtcaaaca cctgactacg tacttcgtcg
     5761 agcatttcgc cggaatcagc catctcttcc tgccacgcaa tcagtttacg cagccaggca
     5821 atccggtctt catgtcccga acgtgcgccg ccagctgccg cgccctcttt atatttccag
     5881 tgcgcagcaa cacccaactc cgcatcttca tgcatctgtt tggtgcggat ttggatctca
     5941 acggtttttc cacccggccc cagaaccacg gtatgaatag actgataacc gtttggtttc
     6001 gggttagcga cgtaatcgtc aaactcatcc ggcaggtggc gatagtgagt gtgcactatc
     6061 cccagtgcgg cataacaatc ctgtaaacgc tcggcgacaa tacgtaccgc acgcacatca
     6121 aacagctcat cgaaggcgag gttctttttc tgcattttgc gccagatgct gtagatgtgt
     6181 ttcggacgac catacacttc agctttaacg ccttcagctt tcatctcagc gcgcagatgg
     6241 ccgacaaact cttcgatata gtgttcgcgg tcgagacggc gttcatgcag cagttttgcg
     6301 atgcgtttgt attcggtcgg gtggagataa cggaagcagt aatcttccag ctcccatttc
     6361 agttgcccaa tcccaagacg gttcgccaac ggcgcgtaga tattggtgca ctcttttgcc
     6421 gccagtacac gttcatcttc cggcgcatct tttacttcgc gcagatgagc aatacgctcc
     6481 gccagtttga tgactacgca gcgaaaatca tcgaccatcg ccaataacat ccggcgaacg
     6541 ttatcgacct gttcggagga aacagaatca gtgtgcgtcg ctttcagctg gcggatcgcc
     6601 gccatatcac gcacgccgtg aataaggtta acaaccgact taccgacgct ctcacgcagc
     6661 acatcttcgc tgactacgtt ggcatcagcc agagggaaca gcagcgccgc ccgcagcgtg
     6721 tcaatgtcca tacttaatgt cgagaggatc tccaccatct caacaccacg ccacaataac
     6781 agactggcat ccggatgccc ctgcgtctgt tgcagacaat acgcccaggt ttcggctaag
     6841 cactcacacg acttctggct ggtaataccc agacttgcga tccatttttc cggatcaaat
     6901 tcaccagcct tattgatatg tgcacttctt accgcaacca tcgtcctctc ctttagggac
     6961 cagacctgcc gaaatcggca aatcgtaact atttaacgcg cgagaaaagt accatcgatt
     7021 ccagatgtcc cgtgtgtggg aacatatcca gcatcgccag tcgcgcaatg gtatatcctg
     7081 cttttaataa cgcttcgcta tcccgagcca gcgttgcagg gttacaggat acataaacta
     7141 tacgaatagg ttccagtttt ataatttgct gcataacacc tgcggcacct gctcgcgccg
     7201 ggtccagcaa cactttatcg aagccgtttt tcgcccacgg ctgctttgtg acatcttctt
     7261 caagattttc gtgataaaac gtcacattct gtaagccatt aagacgcgca ttctgctggc
     7321 ctttttccac cagcgcagga acaccttcta caccgaccac actggcagct tgtgtcgcca
     7381 atggcagtgt aaagttgccc ataccgcaga acagatccag tacgcgatct tcaggttgca
     7441 catccagcca ttccaacgca cgcgctacca ttttttggtt cacacccgca ttgacctgaa
     7501 taaaatcgcg cgggctaaaa gttaagcgca acccgtttga gtcataccag ggcatctcac
     7561 cagagacggt ttcgagtatc tcactatcgg gggcgagata cagatccagg ccttcagaat
     7621 gcgaaaagcg ttccagtttt tcgcgatctg ccgaacttag cggtgcggta tggcgcaaaa
     7681 tcatcagcgt gccgctggtt gcctgtacca gttcaacatg accaagatgg cgcatagctt
     7741 gtaagctgcc cagacatgcc ctgactttgg gcagcaatgc ttcaagttgg ggcgctaaaa
     7801 tggggcattg tttgacgtcg acaatgtcac tggagcccgc tttgcgaaac cccatctgaa
     7861 gttgctgtgt tttcggtaag tagttcagac ttaaacgcgc gcggcggcga tagccccagg
     7921 gaacatcggc gatcacttca gagacatcgt gtttcattaa tcgggcgagt gccgcacttt
     7981 tgcttcgctg ctgtaaatcc acgctggcgt gttgttgctg acagccgccg catacgccaa
     8041 aatgaggaca gcgtggcgtt tcgcgttccg ggctatcgct taaccggcgt acgactttag
     8101 cgcgggcata ctgtttttta tcttcagtaa cagcaacttc cgcgttttcc tgcggcaata
     8161 atccggggat aaataacgtt ttgccgttat gtcgcgccac gccctgacca aaagagtcga
     8221 ggtcgttgac tgaaacggtt atgatctgac gcgtcgtcgt gcgtcgtttt gcagagtaga
     8281 attgcgccat tggcgagact ttctcaattt aacagtgtga ccttaattgt cccataacgg
     8341 aactccatga ccaactacag cctgcgcgca cgcatgatga ttctgatcct ggcaccgacc
     8401 gtccttattg gtttattgct gagtatcttt ttcgtcgtgc atcgctataa cgacttgcag
     8461 cgtcaactgg aagatgccgg tgctagcatt attgagccgc ttgcagtttc tactgaatat
     8521 ggcatgagcc tgcaaaatcg cgaatctatc ggtcagttaa taagcgtact gcatcgtcgc
     8581 cattccgata ttgttcgcgc gatttcggtt tatgatgaaa ataaccgact ctttgtcacc
     8641 tccaattttc atcttgatcc ctcatcaatg cagctcggca gcaacgtgcc gtttcctcgc
     8701 cagctcactg tcactcgtga cggcgatatt atgatcctcc gcacgccgat tatttctgag
     8761 agttactccc ccgacgaatc gcccagtagc gatgccaaaa atagtcaaaa tatgttggga
     8821 tatattgcgc ttgagctgga tcttaaatcg gttcgcttgc agcaatataa agagatcttt
     8881 atttcctgcg tgatgatgct gttttgtatc ggtattgcgc ttatttttgg ctggcgctta
     8941 atgcgcgatg taaccggtcc gattcgcaac atggtgaata ccgtcgaccg catccgtcgc
     9001 gggcaactcg acagccgggt ggaaggattt atgctcggcg agctggatat gctgaaaaac
     9061 ggtatcaact cgatggcaat gtcgctggct gcttatcacg aagagatgca gcacaatatc
     9121 gaccaggcga cgtccgatct gcgtgaaacg ctggagcaga tggaaattca gaacgttgag
     9181 ttggatctgg cgaaaaagcg cgcccaggaa gcggcgcgta ttaaatccga gtttctggca
     9241 aatatgtcac acgagctgcg tacaccactg aatggtgtta ttggctttac ccgcctgacg
     9301 ctgaaaacag aattaacacc aacgcagcgc gatcacctga atacgattga acgttcggca
     9361 aataatttgc tggcaattat taatgatgtt ctcgacttct cgaaactgga agcaggtaag
     9421 ctgattctgg aaagtattcc attcccacta cgcagcacgc tggatgaagt cgttactctg
     9481 ctggcacatt cttctcacga taaagggttg gaactgacgc tcaatattaa aagcgacgtg
     9541 cctgataacg tgatcggcga cccactgcga ttacagcaaa tcatcactaa cctggtgggg
     9601 aatgcaatta aattcaccga gaatggcaac attgatattc tggtagaaaa acgtgcgctg
     9661 agtaatacca aagtgcagat tgaagtgcag attcgggata ccggcattgg tattcctgaa
     9721 cgcgatcaat cgcgcttatt ccaggccttc cgacaggctg atgccagtat ttcccgccgt
     9781 catggtggca ccgggctggg gctggtgatt acacaaaaac tggttaatga aatgggcggc
     9841 gatatttcgt tccatagcca gccgaatcgc ggttcaactt tctggttcca cattaatctc
     9901 gatctgaacc cgaacattat tatcgaaggg ccatccatcc agtgcctcgc aggtaagcgc
     9961 ctggcctatg tcgaaccaaa ctccgcagca gcgcaatgca cgctggatat tttaagtgaa
    10021 acgccgctgg aagtggttta tagcccaacg ttctccgcgc tgcctcccgc gcattacgac
    10081 atgatgttgt taggcatcgc ggtgaccttc cgcgagccgc taacaatgca acatgagcga
    10141 ttagcgaaag cggtatcgat gaccgatttc ctgatgctgg cacttccttg ccatgcacaa
    10201 gttaatgctg aaaaactcaa gcaagatggt atcggcgcgt gtctgctgaa accattaaca
    10261 cctacgcgcc tgttgcctgc cctgacggaa ttttgtcatc acaaacaaaa cacgcttttg
    10321 cctgtaaccg atgaaagtaa gctggcaatg acagtcatgg cggttgatga caaccccgct
    10381 aacctgaaac ttatcggcgc attgctggaa gatatggtgc aacatgtgga actttgcgat
    10441 agcgggcatc aggcagttga acgggcgaaa cagatgccgt tcgatttgat cttaatggat
    10501 attcaaatgc cggacatgga tggcattcgg gcctgcgaac tcatccacca actcccgcat
    10561 cagcgacaaa cgccggttat cgcggtaacg gcgcatgcaa tggccgggca aaaagagaag
    10621 ctgcttggcg cagggatgag cgattatctg gcgaaaccga ttgaagaaga gcgattgcat
    10681 aatttgttgt tgcgctacaa gcctggcagc ggtatttcgt ctcgcgtcgt gacgcccgaa
    10741 gtcaacgaaa ttgtggtgaa cccgaatgcg accctcgact ggcaactggc actacgccag
    10801 gcagcaggaa aaaccgattt agcgcgcgat atgctgcaaa tgttactcga tttcctgcct
    10861 gaagttcgca acaaagttga ggaacagctg gctggagaaa acccggaagg cctggttgat
    10921 ttgattcata aactgcatgg cagttgcggc tatagcggcg taccacgtat gaagaatctc
    10981 tgccagctga tcgaacaaca gctacgtagc ggtactaaag aagaagattt ggagccggag
    11041 ctgctggaac tgttggacga gatggataat gtcgcgcgcg aagccagcaa aattctcggg
    11101 taacggacgt cttgtcgagt agcaatcggc tttttatgac gctggtttca gattggtgac
    11161 aaagtgcctg tgtttatgcc ggatgcggcg ttaacgcctt atccggccta caaaaccaat
    11221 taaattcaat gaattaaaaa atgatgtaga cctgatacat agcacaacag gctattttgc
    11281 gtttagcatc agtctcaaac cggctccaga tagagccggt tttggttttc tgtcttaacg
    11341 caccatgcac gggcgcttgt tatcgaacgt ccagccagga atcagatact gcattcccat
    11401 cgcatcgtca cgcgcgccaa gcccgtgttt ctgatacagc tcatgggctt tcatcacttg
    11461 atccatatcg atttctacac ccagccccgg tttttctggc acctgtacca gcccgccttt
    11521 gatctcaaac ggttctttgg tcaggcgctg attaccttcc tgccagatcc agtgcgtatc
    11581 aatagcagta attttacccg gtgcagcggc ggcaacatgg gtaaacatcg ccagggaaat
    11641 atcgaagtgg ttgtttgagt gtgaacccca ggtcaggcca aattcatggc acatttgcgc
    11701 cacacgtacc gaaccttgca tagtccagaa atgcggatcc gccagcggga tatcaacgga
    11761 ttgtaacgag agcgtatggc ccatctgccg ccagtcggtg gcgatcatat tggttgcagt
    11821 cggcaggcca gtcgcgcgac ggaactctgc catcacttca cgcccggaga aaccttgctc
    11881 cgcaccacac ggatcttctg cataagccag cgaacctttc aggtatttac caattttaat
    11941 tgcttcgttc agcgaccagg caccgttagg atcgagcgta atacgcgcct gcgggaagcg
    12001 ttgcgccagt gccacaatag actcggcttc ttcttccccg gccagtacac cgcctttcag
    12061 tttgaaatcg ttgaagccat atttttcata cgccgcttcc gccaggcgca ccaccgcatc
    12121 cggcgtcatc gcttcttcat gacgcaggcg ataccagtcg catgagtcat ccggctggct
    12181 ttgatacggc agaggtgtgg ctttgcgatt accgacgaag aacagataac cgagcatttc
    12241 gacttcgcta cgctgttgac catcgcccag cagcgatgcc acgtttaccc ccagatgctg
    12301 ccccagcaga tccagcattg ccgcttctat cccggtaact acatgaatag tggtacgcaa
    12361 atcaaatgtc tgcaaaccgc gcccactagc atcacgatcg gcaaaagtat tacgcaccag
    12421 cgtcagaacg tttttgtatt cacccagcgt tttacctacc accagcggaa tcgcatcttc
    12481 cagcgtttta cggattttct cgccgccggg aatttcccct acgccagtgt gaccggaatt
    12541 atctttgata atcacaatat tacgcgtgaa gaacggtgcg tgtgcaccac tcagattcat
    12601 cagcatactg tcatgacccg ccaccgggat gacctgcatt tcagtaacaa caggcgtcgt
    12661 aaattgagaa ctcatattta tgtccttttt cagaatcaat gacggccgaa aacgggacgt
    12721 ttacggtcaa aggtccagcc ggggatcagg tactgcatcg gacctgcgtc gttacgcgca
    12781 ccgcaaggca gacgtttata ggcctcatgt gccttttgta cctgttccca gtccagttcc
    12841 acgcccagac cgggcgcatc aggaacggca atttttccgt ttttaatctc cagcggattt
    12901 ttggtcaggc gacaatcgcc ctcctgccaa atccagtggg tatcgatagc ggtaggatta
    12961 cccggtgccg ccgcgcccac atgggtaaat atcgccagag agatatcgaa atggttgttg
    13021 gaatggcagc cccaggtcag cccccagtcg tcgcaaagct gcgccacgcg gactgcaccg
    13081 gaaagcgtcc agaagtgcgg atcggcaagt ggaatatcta ccgcattgag catcaccgca
    13141 tgacccattt cgcgccagtt ggtggcgatc atgttagtcg cgacgggcaa gccggtcgcc
    13201 cgtcgaaact ccgccatcac ttcacgtccg gagaagccct gttctgcgcc gcatggatct
    13261 tcggcatagg taagaacatc attcagccct ttgcataaag aaatggcttc atcaagcagc
    13321 catgcaccgt tgggatcaac ggtaatccgc gcatccggga agcttttctt caatgcacga
    13381 acagtgtcga tttcttgctc gccaggtaac accccgccct taagtttgaa atctttaaag
    13441 ccatagcgat cctgtgaggc ttccgccaga cgcacaacgg cttcgctgtt catcgctttc
    13501 tgatggcgca actgatacca ctcatggttg cccggcgtat tttccagata aggaagatcg
    13561 gttttggtcc gatcacctat ataaaacaga taaccgagga cggtaatagt ctcgcgttgc
    13621 ttgcctggcc ccaacagttc gcagaccgga acattcagcg ccttacctag caggtcaagc
    13681 aaggcggctt ccagcgccgc cacggcatta acgcgcaatt caaaagtcca ggcacctttg
    13741 ccaaaggtat caaaatcggc tgcctgatta cctttatgta cctgctgaac cactttattc
    13801 aggcgcgcaa cttcctggcc cagaaccatc ggaatagcat cgaccagtgt ttgataaatc
    13861 acctctccgc ccggcgcttc accaatgccg gtatgcccgg cgttatcggt gagtaccacg
    13921 atattgcggg tgaagtatgc gttatgcgca ccaccaatat tgagcaacat gctgtcatgt
    13981 ccggcgaccg gaatgacttt catatcagta ataacaggac tggattgtgt cgccatctta
    14041 ttgccccgcg acaggtttca gctcgatacg cttgatatcg cccaccagca ccaggtagct
    14101 cagtaccgcg attagggcat gaacaccaac ataaatcagc gccccattaa acgagccagt
    14161 cgtgccaacg atataaccaa ttgcgattgg cgtgacgatg ccagaaatgt taccgaacat
    14221 gttgaacagg ccaccggaaa gaccgctgat ctcttttggc gcggtatctg ccattactgc
    14281 ccaacccagc gccccgatgc ccttaccgaa gaaggccagc gccataaagc cgatgatcat
    14341 ccactcaacg ttgacgtagt tgcagaacac catcaccatc gacagcaaca tgcccattac
    14401 gatcggtgtt ttacgcgcaa tgttcagcga tcccgtgcgg cgcatcagcc agtcggaaat
    14461 aattccaccc agcacaccgc cgataaaacc gcaaaccgcc ggaacggaag ccacaaagcc
    14521 cgctttcaga atcgacatcc cacgcgcctg caccagataa accggaaacc aggtaatgaa
    14581 aaagtaagtc agggcgttga tacagtactg accgatataa acgccgatca tcatccgtga
    14641 cccgagcagc tgtttgatct gcccccactt cacgctgaac ggcactttaa ctttggtgct
    14701 ttgctgatcc atattgatca gcgcgccacc cgcggcgata tactccagct ctttctgatt
    14761 tacccccgga tgttgatttg gctcgtggat gactttcaac cagataaagc tgatgacaat
    14821 gcccagaccg cccataaaga agaagacgtg tgaccagccc acttcatgcg tcagccagcc
    14881 cataatcggc gcgaagatca ccgttgcgaa gtattgagcg gagttaaaaa tcgacaccgc
    14941 cgttcccctt tcctgcaccg gaaaccaggc cgcaacaatg cgactgttgc cggggaaaga
    15001 tggcgcttca gcaagcccga ccaggaagcg cagagtaaac agggcaacga taatgccgaa
    15061 tccactaaag atatcgacga agccttgcag caaggtaaac atcgaccaga taaagatcga
    15121 ccagaagtag acgcgttttg aaccaaaacg atccagcaac caaccaccag ggatctgccc
    15181 gataacataa gcccatgaga aggcagagaa cacatagccc attcccacgg gatcaaggcc
    15241 gatatctttg gccatttccg aaccggcaat agaaagcgta gcgcggtcgc cgtagttgaa
    15301 ggatgtgacg ataaacaaca tcaccactat ccagtaacga gcattcgtgc gtttttccac
    15361 actgctcgca gcctgactta aagaactcat tgttgcactc ctgaaaattc gcgttagcca
    15421 cgctcactct ggactgcgac atcgccagga aatcagaggt gacgtagggt gttttttgcc
    15481 gtttttatag gtcgttcgcc gaatacggcg cgtgtttata tctggcaata gcagtataaa
    15541 aagcgcgcca tagcggctca ccgtgcaaca acacaacatt aatgcgttca atgaggcccg
    15601 attttggcat tagccctgga cggtggaatc cacttcacgg aaatgaaaac aagaacaaga
    15661 aaggaagggt aaaaacgaag aaataaagaa gagtatgaaa tggatcgctt gactctaggc
    15721 aaacgccagt aaaaatccgc gctatgaagc agtttttact ggcatttgcc tgaaaagatt
    15781 cgattcagca ccgctaaaac gacatttacc gctcgctgac atatcaggac aacagcgtgc
    15841 cccactgttc gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa
    15901 tcaacagcat ttcgccaacc cgctgagcgc tctgttcctg caacaaggca tcgaactgtt
    15961 tgccgccatt gcaaaaattt acataactgc tgtcgccgag cgcaatcacg ccataacgca
    16021 gattcggctg gaagcccaga ctatctttga ttccctgaaa gagcggcaca atgctatcag
    16081 gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgatagggca
    16141 gccagtcgct taattcagga tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa
    16201 ttgcttctgc ttcttccgcc actaacagcg aatttccgta catggtgccg acaaaaatac
    16261 caatttccgc cataccgttt tctccctgga ttaggaactt atctctccat cctgacccga
    16321 tggcgcactg aactcaaccc tttcattttc aggaagcaga ccgcgccagc caaattgtga
    16381 taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct cgccagtaaa
    16441 aggatgcgtc aatgacaact gactggcatg tagcattaac cgctggaggc caaaatgctc
    16501 agcaccgctg cgattctggc gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg
    16561 caaatgggca agatgtcgtc gaagctgatg tttgcgtccg gttttcggct ccagttccac
    16621 caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag
    16681 accgcgataa tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc
    16741 gattttgtcc agttcttcca ccagtggata atccaacacc gcttcttcca tcaaccagcc
    16801 gcgcacaatc gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag
    16861 ccgtccggcc tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg
    16921 atgagcagta aaaacatgct gtcctatctg gtcacgcacg gtttgcatga ccactacttt
    16981 ctcgtcgcga tccagccagc tacggtgaac cagccagccg gagggtttat ttaccgcaac
    17041 cagccattca tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca
    17101 tccagttttt ccaactcagc cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc
    17161 atttcataat agggtgcaac agcaaaagcg cccggtaacg gctggttgtt atttaacaaa
    17221 tcgtgcattc gcgggatcag cccccactgc aaccactcca gtggttccat ggtgtccata
    17281 aagaacggtt gggtactatt aaattgatgc ggctggggtt catcgtttcg ccagtgctga
    17341 tgttcacgca gtaacgcttc aagcgcctgc aactggagac gaacgcggtc atgagtggtc
    17401 ataaagcaac ctcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc
    17461 actgtattca cagtgctccc ggttcgtttc gcagcattcc agctactttt gttgctccct
    17521 gctcatcctt gacaactttt cctctggcct tgcggccaat cgttcatcct gaactattgc
    17581 ttcctgctca caccaccccg atgtgatact tcatcctgaa gtgtccctgg ccttcctgac
    17641 ccaccgaatc atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc
    17701 tgcgtcatcc tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc
    17761 tgcagaagtg tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata
    17821 gaatcgtctt tttcgcgaag tcttacaagg cgcttacaga caatgcctta aggaaaattt
    17881 catatgaaag ttaagaatta acaatcaaca ttttgatttt aaatgaaaat aacgattctt
    17941 atctttttaa gtattcgctt atttcctggc gatctcctac agagtctgta agagatctct
    18001 cacaaccaga tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca
    18061 agatttgagg ctaaattcgt ccgtttacgt gttcccagcg tctctttgca cacttcaccg
    18121 gagagattac acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg
    18181 ggcggcagtt ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga
    18241 cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg
    18301 tgcatatccc cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg tatcacaata
    18361 tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag
    18421 tatacggcat cttcggtagt tgaaataatg cacggtgaag gaacaccgta caattcctca
    18481 cttaacggcc agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct
    18541 ttcagggctt gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac
    18601 ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag
    18661 gcacttgcgg gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc
    18721 ctactgcaag gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac
    18781 ctgccttttc acggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa
    18841 ggtttgccgc aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt aaatctgatt
    18901 gagtcgaaga gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg
    18961 gatgaggtgc gccagacgct ggagcgcgac ttaagcactt gcgctcaggg taagattagc
    19021 gtggcgttat atcgtcttga tgaactggaa ggccagccga taggtcattt caatggcact
    19081 tgcattgatg accaggatat cactatcgat aactatgaat tcactactga ctatctggag
    19141 aatgccacca gcggtgaaaa agtagtggaa gagacgctcg tcagccacct gctgaaatca
    19201 aactgcctga tcacccatca accagattgg ggttcgatcc aaattcagta tcgtggacgc
    19261 caaattgaca gagaaaaact gctgcgttac ctggtctcat tccgtcatca caacgagttc
    19321 cacgaacagt gcgtggaacg catctttaat gacctgttac gcttctgcca gccagagaaa
    19381 ttgagcgttt acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt
    19441 aatagcgatt ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt
    19501 tgcgtgctgg attcacgcag aaggttgtga aaggtcatca ggcagggcta ttgtaatcaa
    19561 agggaatgac gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt
    19621 ggctccatgg atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc
    19681 gacctctatc aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc
    19741 gataacagca aagaattgct gtctcgtttt gaaaatttcg atattaacgt cctgcgccgt
    19801 gaacgcggcg taaagctgga actgattaat cccccggaag aggcttttgt cgatgggcga
    19861 attattcgcg ctttgcaggc caacttgttc gcggttctgc gagacattct cttcgtttac
    19921 gggcaaatcc atcataccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc
    19981 actaacctgg tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg tgaagcgcca
    20041 aatatggtgg tctgctgggg cggtcactca attaacgaaa acgagtattt gtatgcccgt
    20101 cgcgtcggga accagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccagga
    20161 gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg ttacaaagac
    20221 agtcgtttta ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccactg
    20281 gtcaacgaat tgatcatcat gccggatatc gaaaaacgtc tggaagcgtt tgtccgtatc
    20341 gctcacggca tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg
    20401 ctgggaattt taatgaaccc ggccaacaaa gatcaggttt taccattgat cctcaccggc
    20461 ccgaaagaga gcgccgacta cttccgcgta ctggacgagt ttgtcgtaca tacgctgggc
    20521 gaaaacgcgc gccgccatta ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag
    20581 atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgtg atacaggcga tgcctacagc
    20641 tttaactggt caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc gtctcacgag
    20701 aatatggcta atctgaagct ttacccggat caacctgttg aagtgctggc tgccgacctg
    20761 cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt
    20821 gaagagtttg gtccttacaa aatcaacggc gataaagaga ttatgcgtcg tatggacgac
    20881 ctgctacagg gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct
    20941 tgctacgaaa tctgcacgta atctcccctc ccc
//
LOCUS       DAJTYL010000068        20797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYL010000068 DAJTYL010000000
VERSION     DAJTYL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.068"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..686
                     /locus_tag="OO572_002460"
     CDS             <1..686
                     /locus_tag="OO572_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR6661432.1"
                     /translation="RYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSY
                     RGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLS
                     ERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGN
                     CLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEY
                     RNQTYMPRV"
     gene            788..940
                     /gene="hokA"
                     /locus_tag="OO572_002461"
     CDS             788..940
                     /gene="hokA"
                     /locus_tag="OO572_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6661433.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1127..1339)
                     /gene="cspA"
                     /locus_tag="OO572_002462"
     CDS             complement(1127..1339)
                     /gene="cspA"
                     /locus_tag="OO572_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6661434.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1620..1910)
                     /locus_tag="OO572_002463"
                     /pseudo
     CDS             complement(1620..1910)
                     /locus_tag="OO572_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2344..3054
                     /locus_tag="OO572_002464"
     CDS             2344..3054
                     /locus_tag="OO572_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6661435.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3104..4078)
                     /gene="ghrB"
                     /locus_tag="OO572_002465"
     CDS             complement(3104..4078)
                     /gene="ghrB"
                     /locus_tag="OO572_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6661436.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4182..4832)
                     /locus_tag="OO572_002466"
     CDS             complement(4182..4832)
                     /locus_tag="OO572_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6661437.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4995..7193
                     /locus_tag="OO572_002467"
     CDS             4995..7193
                     /locus_tag="OO572_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6661438.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7162..7602)
                     /locus_tag="OO572_002468"
     CDS             complement(7162..7602)
                     /locus_tag="OO572_002468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6661439.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7599..8162)
                     /gene="tag"
                     /locus_tag="OO572_002469"
     CDS             complement(7599..8162)
                     /gene="tag"
                     /locus_tag="OO572_002469"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6661440.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8320..9018
                     /locus_tag="OO572_002470"
     CDS             8320..9018
                     /locus_tag="OO572_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6661441.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9247..10455
                     /locus_tag="OO572_002471"
     CDS             9247..10455
                     /locus_tag="OO572_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661442.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10779..12470
                     /gene="eptB"
                     /locus_tag="OO572_002472"
     CDS             10779..12470
                     /gene="eptB"
                     /locus_tag="OO572_002472"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6661443.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12562..12638
                     /locus_tag="OO572_002473"
     tRNA            12562..12638
                     /locus_tag="OO572_002473"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12596..12598,aa:Pro,seq:cgg)
     gene            13382..14989
                     /gene="dppA"
                     /locus_tag="OO572_002474"
     CDS             13382..14989
                     /gene="dppA"
                     /locus_tag="OO572_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6661444.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15297..16316
                     /gene="dppB"
                     /locus_tag="OO572_002475"
     CDS             15297..16316
                     /gene="dppB"
                     /locus_tag="OO572_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6661445.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16326..17228
                     /gene="dppC"
                     /locus_tag="OO572_002476"
     CDS             16326..17228
                     /gene="dppC"
                     /locus_tag="OO572_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6661446.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17239..18222
                     /gene="dppD"
                     /locus_tag="OO572_002477"
     CDS             17239..18222
                     /gene="dppD"
                     /locus_tag="OO572_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6661447.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18219..19223
                     /gene="dppF"
                     /locus_tag="OO572_002478"
     CDS             18219..19223
                     /gene="dppF"
                     /locus_tag="OO572_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6661448.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19253..20524)
                     /locus_tag="OO572_002479"
     CDS             complement(19253..20524)
                     /locus_tag="OO572_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6661449.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5034 a         5113 c         5439 g         5211 t
ORIGIN      
        1 gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa cagattaacc
       61 ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt aaggtcaagc
      121 gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc caaagagatt
      181 tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt gcagtcaatg
      241 ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca
      301 gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag gcattcaaaa
      361 agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga
      421 gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca
      481 attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt
      541 atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa tatattgaat
      601 actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga
      661 atcagaccta tatgcctcgt gtataactgt ccaacttttt ggggtcagta caaagtgagg
      721 ctaccctatg cctgaacacc ataaggttag cctcttactc gttggaaatc aacacagggg
      781 gctgggaatg ccgcagaaat atagattact ttctttaata gtgatttgtt tcacgctttt
      841 atttttcacc tggatgataa gagattcact gtgtgaattg catattaaac aggagagtta
      901 tgagctggcg gcgtttttag cctacaaatt gaaagagtaa gagtcttcga cgggaaatta
      961 ttcccgcctt acttacggcg ttgcgcattc tcattgcacc catatttatt cttcacaaaa
     1021 ataataatag attttattac gcgatcgatt atttatttcc tgaaaaaaaa taaaaaaatc
     1081 cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta
     1141 cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt
     1201 cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acatctttag
     1261 agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac
     1321 cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata
     1381 tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga
     1441 taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg
     1501 atgtgcatta agccactcat tggcgggtga tgcaacaatt atttttcata tttatgatta
     1561 atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg gataaaagtc
     1621 tatttcatca actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt
     1681 tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg
     1741 cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcataa ctgctcaatt
     1801 tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca
     1861 atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc
     1921 tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag
     1981 cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca gcctgtcatt
     2041 tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct tatcactcct
     2101 gttttgtaaa gatttcgcaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa
     2161 aaggcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc
     2221 tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta
     2281 tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag
     2341 aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg
     2401 gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac
     2461 ttcctgcaaa atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag
     2521 aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag
     2581 gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa cgcaattcgc
     2641 gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt
     2701 gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta
     2761 aaacagagcg atgacctgaa accggtattt gatcaggcat tcaccaaagt ggtaacgacg
     2821 ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca acaactggtg
     2881 atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc aaatggcatc
     2941 cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca
     3001 caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta
     3061 aaagcccgag cgattatcgg gctttacttc acgcagtcgc agcttagtcc gcgacgtgag
     3121 gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta tccacggcac
     3181 aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga
     3241 cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg aagacatcca
     3301 gcccggcggc gtgaatttcg cctttctgca atgctgcgat cagtgcattt tcgtcaacca
     3361 ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg aattgttctg
     3421 cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg aaatctgact
     3481 cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat
     3541 ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcaca cgttgtgcca
     3601 gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc
     3661 cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct
     3721 ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg acggtttctg
     3781 ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa
     3841 aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca
     3901 atgcggcatc tacattctcg tttgaaccca gtaaaccttc agcttctgca aaaattgctg
     3961 cattttgaac gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgcgctt
     4021 gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc
     4081 ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga
     4141 agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta
     4201 atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta
     4261 gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg
     4321 ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg accccccgtg
     4381 ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc
     4441 acgccggtca gggtgttagc gcctgccggt ttcaggggag cgctgctgct gtcgaaggtc
     4501 acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct aacaccagtg
     4561 ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg
     4621 cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa
     4681 gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat
     4741 ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg
     4801 ccactcacta cggcggcaag taagataaac acgtttcttc attgttaatc cttaataacc
     4861 tttttattct ttgtcacggg ttccgtggcg ggagattatg ccgcgtgaac atgaagatta
     4921 ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca tctaaccttt
     4981 caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact gccgcccact
     5041 gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt ggcgcgttag
     5101 ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac agcaatacgc
     5161 gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca
     5221 ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggca ggcggttata
     5281 ccgggcatct gggggattat tcgaccggtg cggcacaggc gatcatgccg tatgtcgtag
     5341 gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg
     5401 tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatccg
     5461 atgagcaggg gctttcttac ttttccgcac tgcgtgacag tgggaaaaag ctgatctgca
     5521 ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag tgggtggcac
     5581 cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg
     5641 gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt
     5701 atttgctggg cgagagtgac ggaatagcga aaaacgccga atgggcggca gagatttgtg
     5761 gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat accaccatgc
     5821 tgatggcagg ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgatcg
     5881 tcacgctggc agcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt
     5941 accattttgc caatggtggt gaccccacgc ggcgttctgc ggtgctctct tccatgcagg
     6001 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     6061 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     6121 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     6181 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     6241 cggcgaaaca cgcggatatt gttctgcctg cgactacctc ttttgagcgt aatgatctca
     6301 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     6361 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     6421 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     6481 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     6541 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     6601 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     6661 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     6721 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     6781 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     6841 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     6901 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     6961 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     7021 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     7081 ccagctcgcg gctggggaat ggctgtgcgg gtaatacgac gctggcatgg ctggaaaaat
     7141 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     7201 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     7261 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     7321 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     7381 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     7441 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     7501 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     7561 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccagatag
     7621 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     7681 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ccttagatag ggcgtcggag
     7741 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     7801 tttacaaacg accagacaaa gtcgggaaac ggttcgccgt tctgttccat ttgcaggtac
     7861 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     7921 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     7981 tgaaagtagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     8041 tgctgccctt caaagcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     8101 cactcattat catggtaggc aatataaagc gggccctgac tcacccagcc gcaacgttcc
     8161 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     8221 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     8281 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     8341 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     8401 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     8461 tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa
     8521 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     8581 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     8641 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     8701 actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg ctggcgtgtt
     8761 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     8821 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     8881 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     8941 gctgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     9001 gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac aacagttgtg
     9061 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     9121 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaagc
     9181 ataaaatgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     9241 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     9301 attacccagt ttgcgctggg gtcggtttat acccggagcc tgtttaatgg cgcgctttct
     9361 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     9421 ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac gttttggcgt taaacgcgtc
     9481 accgtggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     9541 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     9601 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     9661 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     9721 ctcgaaacgg tgggtctgga aaaaaccttt gtgatttggg gagcgattgc gctggtgatg
     9781 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     9841 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     9901 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     9961 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
    10021 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
    10081 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
    10141 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
    10201 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
    10261 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
    10321 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
    10381 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
    10441 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
    10501 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
    10561 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
    10621 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
    10681 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgctaa
    10741 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
    10801 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
    10861 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
    10921 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
    10981 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
    11041 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
    11101 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
    11161 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
    11221 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggtggtcgtc gtactggcgg
    11281 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
    11341 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
    11401 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
    11461 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgata
    11521 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
    11581 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
    11641 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
    11701 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
    11761 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
    11821 tggcagacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
    11881 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
    11941 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
    12001 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
    12061 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
    12121 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
    12181 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
    12241 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
    12301 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
    12361 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
    12421 acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa gcggcctgct
    12481 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
    12541 cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact tcgttcggga
    12601 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
    12661 agcaggtttt tttgcatttg tagctcatga ggatgagaac ctccgggggc agggaggctc
    12721 gactcgacgt aggcctgata agacgcgcca gcgtcgcatc aggcaacggc accgcactta
    12781 ttgtcggatg cggcgcgacc gccttattcg accaacacac cacacatttt cctgccaact
    12841 ttgcgcccca tatctcacta tccattaacg gatttgtgac agaaaccatt gtcttttttt
    12901 atatttgctt aaatcttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg
    12961 ttttagcgtt catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt
    13021 aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg
    13081 ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt
    13141 tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt
    13201 tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt
    13261 aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt
    13321 caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat
    13381 aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat
    13441 gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga
    13501 agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtcccgct
    13561 ttataaccgc ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg gcctcgctga
    13621 aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa
    13681 gtggcacgac aataaagaat tcaaaccgac gcgcgaaatg aacgccgatg acgtggtgtt
    13741 ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg gcggcagcta
    13801 cgaatacttc gaaggtatgg gcttgccgga gctgatcagc gaagtgaaaa aggtggacga
    13861 taacaccgtt cagtttgtgc tgactcgacc ggaagcgccg ttcctcgctg acctggcaat
    13921 ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag ccggtacacc
    13981 ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa
    14041 agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga
    14101 tacgctggtc ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa
    14161 tgaatgccag gtgatgccgt acccgaaccc ggcagacatc gctcgcatga agcaggataa
    14221 atccatcaac ctgatggaaa tgccggggct gaacgtcggc tacctctcgt ataacgtgca
    14281 gaaaaaaccg ctggatgacg tgaaagttcg ccaagcacta acctacgcgg tgaacaaaga
    14341 cgcgatcatc aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc
    14401 aaccatgtgg ggctataacg acgacgttca ggattacacc tacgatcctg aaaaagcgaa
    14461 agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc
    14521 ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg
    14581 ggcgaaagtg ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg
    14641 tgcgaaagat ggcgaacatc agacggtaat gatgggctgg actggcgata acggggaccc
    14701 ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt ctaactactc
    14761 aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca
    14821 taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc aggctccggc
    14881 actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtca aaggctatgt
    14941 ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat
    15001 acaagactga tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa
    15061 ttctgcaatg tattgaattt gcacgatttt gtaggccgga taaggcgtta acgccgcatc
    15121 cggcataaac aaagcgcact ttgtcaacaa tctgtatacc cggtggcgct gtgcctctgc
    15181 gtgcagcgcc atccggcagc aatacttcat tctctgtccg acaagggcgg gaattgattt
    15241 gtgagcaata cagacacgca gttccaggct gcgagtcact atagagaatc cgggttatgt
    15301 tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc
    15361 tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcaggcgaac
    15421 gtgggatctc ccctgagcgt cacgtgcagc tgctggctga actcggctta gataaaccga
    15481 tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgatcta ggcatttcaa
    15541 tgaaaagccg aattccagta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac
    15601 ttggcgtctg cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg
    15661 cggttaaacg cggttccatt ttcgatcaca ctgcagttgg cctggcgctg accggctact
    15721 cgatgccgat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc
    15781 tgacgcccgt ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa
    15841 ccggttttat gctaatcgac accgccatct ggggtgaaga cggcaacttt atcgatgccg
    15901 tcgcccatat gatcttgccc gccattgtgc tgggtactat tccgctagcg gtcattgtgc
    15961 gtatgacacg ctcctcgatg ctggaagtgc tgggcgagga ttacatccgc accgcgcgcg
    16021 ccaaagggct aacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc
    16081 cggtagtgac cgttatcggc ctccaggtgg gaacattgct ggcgggggcg attctgaccg
    16141 aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg caacgccgcg
    16201 actatccggt agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaact
    16261 tgctggtcga tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg
    16321 acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt
    16381 tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg
    16441 tcgccatcgt gctgttcatc gcgatctttg ccaactggat tgcgccttat aacccggcgg
    16501 atcagttccg cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc agtatggcac
    16561 acttgctggg caccgatgac gtcggtcgtg atgtgctgtc gcggctgatg tacggcgcgc
    16621 gtttatcgct gctggttggc tgtctggtgg tggtgttgtc gctgattatg ggcgttattc
    16681 tcggcctgat cgccggttac ttcggcggcc tggttgataa cattatcatg cgcgtggtcg
    16741 atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc
    16801 cgtcgattgg taacgccgcg ctggcgctga ccttcgttgc cttgccgcac tatgtgcgct
    16861 taacccgcgc cgccgtgctg gtggaggtga atcgcgatta cgtcaccgcc tctcgcgtgg
    16921 caggtgccgg ggcgatgcgc cagatgttta ttaacatctt cccgaactgc cttgcgccgc
    16981 tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt
    17041 tcctcggcat gggggcacag ccgccaacgc ctgagtgggg caccatgctc tccgacgtgt
    17101 tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc tggtctggcg atcctgttga
    17161 cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactaa
    17221 agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc
    17281 gacgaaagcg caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa
    17341 gtggtcggga ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact ggcgattatg
    17401 gggctgattg attatccggg ccgcgtaatg gcagaaaaac tggagtttaa cggccaggat
    17461 ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc
    17521 ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca gattatggaa
    17581 gcgattaaag tgcatcaggg cggcaacaaa agtacccgcc gtcagcgggc gattgacctg
    17641 ctgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc gcatcagctt
    17701 tccggcggca tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg
    17761 ctgattgccg atgaaccgac caccgcgctg gacgtgacca ttcaggcgca aatcatcgaa
    17821 ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg
    17881 gcgctggtgg cggaagctgc gcataaaatc attgtgatgt atgcaggtca ggtagtagaa
    17941 acgggcgatg cacacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg
    18001 cgtgcgctgc cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgttgtt
    18061 cccggcaagt atgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact
    18121 gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgatgggcg tcagtccaaa
    18181 tgccattacc cacttgatga tgccgggagg cccacactat gagtacgcaa gaggccacct
    18241 cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca
    18301 tgttcgtgcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac cttgaacgtg
    18361 gcaaaacgct ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc
    18421 tgacgatgat cgaaacgccc accggcggcg agctgtatta tcaggggcag gatctgctca
    18481 agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaatc
    18541 cctacggttc gctgaatccg cgtaagaaag ttgggcaaat tctcgaagag ccgctgctta
    18601 ttaacaccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcaaaag
    18661 tcggcctgaa aaccgagcac tatgaccgct atccgcacat gttttccggc ggacaacgtc
    18721 agcgtatcgc tattgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgagc
    18781 cggtttctgc gctggacgtc tcagtgcgtg cgcaggtgct gaatctgatg atggatttgc
    18841 agcaggagtt ggggctttct tatgtcttta tctcccacga cctgtcggtt gtggagcaca
    18901 ttgctgatga agtgatggtg atgtacctag gccgctgcgt ggagaaagga acgaaggacc
    18961 aaatcttcaa taacccgcgc catccgtaca cccaggcgct gctctccgcg acgccacgcc
    19021 tgaacccgga cgatcgtcgt gaacgcatca aactcaccgg tgaactgcca agcccgctga
    19081 atcctccgcc gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc
    19141 agttgcagcc gcagctaaaa gactacggag gtcaactggt agcttgtttt gctgttgatc
    19201 aggatgaaaa tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa
    19261 tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc agccaggctt
    19321 taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc
    19381 acgacacaat gccatataac gggcttccca actgaaagaa caccagcact gatacacgaa
    19441 acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcatgc
    19501 gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa
    19561 tagcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca
    19621 gcaccgtaga ggtgatatga atgatatgtc cagggatcac ctgcgcggcc agtgccagtg
    19681 ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg
    19741 aaaaggcaaa aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca
    19801 gcgcgagccg ggttgccagt actttatctg cttcccgttt acgataggca atattcattg
    19861 ggttaagtac ctgaataaat accgcagaaa agaagcaaaa tggaatggta agcaggacat
    19921 cgcggaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga
    19981 tcatcgcaaa accgaacacg acaataatcc ctactttgac caccaccatt ggcccagaaa
    20041 tcttaaacag taatcgttca ccaccagacg caatcgctat cagtacggcg aaaatagcga
    20101 ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg
    20161 aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa
    20221 agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg
    20281 tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc
    20341 aggtgacagg ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa
    20401 tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc
    20461 agccgaagtc gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct
    20521 gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct
    20581 tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact
    20641 ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt
    20701 cagcccggat atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc
    20761 aatgaggaag attgccgggt gctgacagta aaaagca
//
LOCUS       DAJTYL010000069        20689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYL010000069 DAJTYL010000000
VERSION     DAJTYL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.069"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..2023)
                     /gene="glyS"
                     /locus_tag="OO572_002480"
     CDS             complement(<1..2023)
                     /gene="glyS"
                     /locus_tag="OO572_002480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6661450.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMADDKELRLNRLTMLEK"
     gene            complement(2033..2944)
                     /gene="glyQ"
                     /locus_tag="OO572_002481"
     CDS             complement(2033..2944)
                     /gene="glyQ"
                     /locus_tag="OO572_002481"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6661451.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3039..3338)
                     /locus_tag="OO572_002482"
     CDS             complement(3039..3338)
                     /locus_tag="OO572_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6661452.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3513..4508
                     /gene="wecH"
                     /locus_tag="OO572_002483"
     CDS             3513..4508
                     /gene="wecH"
                     /locus_tag="OO572_002483"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6661453.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4550..4987)
                     /locus_tag="OO572_002484"
     CDS             complement(4550..4987)
                     /locus_tag="OO572_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6661454.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5033..5374)
                     /locus_tag="OO572_002485"
     CDS             complement(5033..5374)
                     /locus_tag="OO572_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661455.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5543..6997)
                     /gene="xylB"
                     /locus_tag="OO572_002486"
     CDS             complement(5543..6997)
                     /gene="xylB"
                     /locus_tag="OO572_002486"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6661456.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7069..8391)
                     /gene="xylA"
                     /locus_tag="OO572_002487"
                     /pseudo
     CDS             complement(7069..8391)
                     /gene="xylA"
                     /locus_tag="OO572_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8757..9749
                     /gene="xylF"
                     /locus_tag="OO572_002488"
     CDS             8757..9749
                     /gene="xylF"
                     /locus_tag="OO572_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6661457.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9827..11368
                     /gene="xylG"
                     /locus_tag="OO572_002489"
     CDS             9827..11368
                     /gene="xylG"
                     /locus_tag="OO572_002489"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6661458.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11346..12527
                     /gene="xylH"
                     /locus_tag="OO572_002490"
     CDS             11346..12527
                     /gene="xylH"
                     /locus_tag="OO572_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6661459.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12605..13135
                     /locus_tag="OO572_002491"
     CDS             12605..13135
                     /locus_tag="OO572_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6661460.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13247..13783
                     /locus_tag="OO572_002492"
     CDS             13247..13783
                     /locus_tag="OO572_002492"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6661461.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13891..14715)
                     /locus_tag="OO572_002493"
     CDS             complement(13891..14715)
                     /locus_tag="OO572_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6661462.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15035..17065
                     /gene="malS"
                     /locus_tag="OO572_002494"
     CDS             15035..17065
                     /gene="malS"
                     /locus_tag="OO572_002494"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6661463.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17243..18496
                     /gene="avtA"
                     /locus_tag="OO572_002495"
     CDS             17243..18496
                     /gene="avtA"
                     /locus_tag="OO572_002495"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6661464.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18647..19120)
                     /locus_tag="OO572_002496"
     CDS             complement(18647..19120)
                     /locus_tag="OO572_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6661465.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19222..20070)
                     /gene="yiaJ"
                     /locus_tag="OO572_002497"
     CDS             complement(19222..20070)
                     /gene="yiaJ"
                     /locus_tag="OO572_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6661466.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20270..>20689
                     /locus_tag="OO572_002498"
     CDS             20270..>20689
                     /locus_tag="OO572_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCR6661467.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNS"
BASE COUNT         5210 a         5265 c         5067 g         5147 t
ORIGIN      
        1 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcagcc atgaccatca
       61 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
      121 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
      181 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcgatcg ctcagcactt
      241 cgtcagattt cgccaggatg ttagatacac gtttgttcgc cgccgccagt gctgcagctg
      301 cttccagggt acggaagtgc gataccgctt tcatgcgggc atcgaaatca gccggacgag
      361 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt ataaccttcg tcctgatacc
      421 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg gcattagtca
      481 gcttatcgcc atacagacgc accgcttctt cggtcagcgt ttgcagatca aggttgaggt
      541 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
      601 tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc atcttatcag
      661 caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca aagcgcggct
      721 gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga cgcgcgtagt
      781 gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
      841 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcggcga
      901 tccagccagc cagcgcctgg atgcggtcag tcttgtcacg cagcgtaccc agctgttgct
      961 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgttttttg cggtcggtgt
     1021 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     1081 tttgctgcgg atctttcgat tcgatgttgg caacgaagat aaagttcggc agcagtttgc
     1141 cgtcgttcgc atacaccggg aagtacttct ggtcaccttt catggtgtaa accagggctt
     1201 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     1261 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     1321 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     1381 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     1441 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     1501 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     1561 cgtcgcttgc accccagcgc atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa
     1621 ccatattcgg cagcagtgct tcggtgcttt cgcccttcac atgggcgcga tacagcagcc
     1681 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaatacca caaccacgcg
     1741 cccaaccttc tgccgctttg ctcggttttc cttcagcgtc gaacgcctgg gcaatcgccg
     1801 ggccgcgttt ttcgatttca cgatctggtt gtgcttccgc caggttagct actttcagcg
     1861 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcct gcgttatcca
     1921 gctccgcagt aaagttcgca gcaaaggact ctgccaggct gcgcagtgct tttggtggca
     1981 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     2041 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtaatatgct tctgccactg
     2101 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg gagatggctt
     2161 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     2221 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcaaagc
     2281 aggtgaacag gaagtccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     2341 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagactaggt
     2401 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     2461 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     2521 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcccccaga gtcgggtttt
     2581 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     2641 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     2701 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     2761 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     2821 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     2881 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     2941 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     3001 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     3061 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     3121 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     3181 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga
     3241 tcaaccctta cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc cactagcacc
     3301 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     3361 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     3421 tataggctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     3481 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     3541 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     3601 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     3661 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     3721 gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg
     3781 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     3841 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     3901 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg
     3961 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     4021 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     4081 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     4141 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     4201 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     4261 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     4321 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     4381 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     4441 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     4501 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     4561 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct
     4621 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     4681 cactgagtaa taatgtcgca ttccacagac ctaccatcag tagagcaaca gagataataa
     4741 agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca tacttgtccc
     4801 gaacggtctt ttgataagac gcagcagaaa acagtcctaa caccagtacg gcaaaataat
     4861 accctttttc atttaactgc atctctgcat tccatagccc taacaggtag gtaacgatac
     4921 caccaacgag agctatccat gacacaatac taaaggccgg tgaataggtt gatattttgt
     4981 tgtccatcac agtattcctt tcattcctga atgtgtaaga gctttatgtt gctcataccg
     5041 agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca
     5101 tttcataagc attgataggt gcatttaaaa caccgataaa caagagaccg atagtgatta
     5161 acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc
     5221 gaagatatgc atagttacca aaagttgctg tcattaacac gccaagaaaa tagccttttc
     5281 cacttagcaa tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa
     5341 ataatagttt tgccacagtt tttgaggttt tcataacgat ctccatatct accagcgata
     5401 cattacgagt aaccaacgaa agacaaaact gaaaaatgcc attaacaaat gattttcaga
     5461 ataaattcat actaaatatt aattaattag tgagatatat agatgtgaat tatcccccac
     5521 ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga tagaggcgac
     5581 ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt agatgcgact
     5641 gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc gccgcaatct
     5701 gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg taatcgagct
     5761 gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcgcc ccgccaccaa
     5821 tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg ccatctgcca
     5881 gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca tgttgatgag
     5941 tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc tcgccggaaa
     6001 gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct gcagcgatta
     6061 aagctgggac attgcacagg ccggttaatt tcgcggccca atccagacac gatgctgcac
     6121 tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag ctgtgtacag
     6181 cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc gacgtcccca
     6241 gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca gctgcattgt
     6301 cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca acttcaggta
     6361 acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca cgagataagt
     6421 cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc cacatggtgc
     6481 cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc aagtaatctt
     6541 tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc tgaacccata
     6601 gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact cgtgattgcg
     6661 gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg
     6721 cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc tggccggcaa
     6781 tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc attgcgcgat
     6841 cagttgcctg ccaccactgt tccgggtctt gttccgacca aagtggatgc gggcgcgaaa
     6901 cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc aaaataactt
     6961 ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct taaaaaaatg
     7021 cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt atttgtcgaa
     7081 cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat gcaccggaga
     7141 caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc ctttcaggat
     7201 ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt tatccagctc
     7261 gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat ccatcgcgcc
     7321 gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt tggcatcgaa
     7381 gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca ccagcgcatt
     7441 ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat cgccacggtt
     7501 ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat gatggaaaga
     7561 gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt tttccagacc
     7621 aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat gtttagtcgg
     7681 ttcttgcggt ttcggttcga taagcaacgt gccctagaag ccgattttat gtttatgctc
     7741 aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt cggtatttaa
     7801 cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc ccaatttatg
     7861 ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt caggatctgg
     7921 gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg ttccccacag
     7981 cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt gcgcaaaatt
     8041 attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt ggaagcaata
     8101 aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac gcttcgccaa
     8161 cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac caaacatgtc
     8221 cgccccgttc cagcagaagg tgtgccagta gcaggcggca aaacgcaagt gctcttccat
     8281 acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg ggtttgagga
     8341 ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca tattgaactc
     8401 cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac tccctttcaa
     8461 ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg cgctcgctcg
     8521 caaaataaaa tgggatgatg aaaatgggta attcctcgaa gataaaaatg caataagtac
     8581 aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa aaatcgtaat
     8641 cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt
     8701 atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc tacaccatga
     8761 aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac gttgcggcac
     8821 acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa cgctggcaaa
     8881 aagatcgaga tatttttgtg aaaaaggcag aatctctcgg tgcgaaagta tttgtacagt
     8941 ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgatt aaccggggtg
     9001 tcgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt gtaaaaaaag
     9061 ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat gcggatatcg
     9121 atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa gccttggtcg
     9181 atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat aacaacgcca
     9241 agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattct ggaaaaatta
     9301 aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg aaaattatgg
     9361 aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca
     9421 ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt
     9481 ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga
     9541 cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg
     9601 gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct
     9661 cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag
     9721 acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg
     9781 atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact
     9841 tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt
     9901 gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac
     9961 gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt
    10021 tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat
    10081 cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg
    10141 taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac gctatcagaa
    10201 gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct
    10261 tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat
    10321 tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat
    10381 tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt
    10441 caaagcgatt tccgatacga tttgcgttat tcgcgacgga cagcacattg gtacgcgtga
    10501 tgctgccgga atgagtgaag acgatattat caccatgatg gtcgagcgag agttaaccgc
    10561 gatttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac
    10621 ggcatggcat ccggtcaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa
    10681 acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca
    10741 gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca
    10801 ggtagatatt cgtaactgtc agcaagccat cacccagggg attgcgatgg tacccgaaga
    10861 cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc
    10921 actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat
    10981 tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgaccttg ctattggacg
    11041 tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac ttaacccgcg
    11101 catcctcatt cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta
    11161 caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt
    11221 acctgaagtg ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc
    11281 caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga
    11341 acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct
    11401 tttctgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa
    11461 tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca
    11521 acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa
    11581 tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg
    11641 cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc
    11701 tgggactgct tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa gtcccttcat
    11761 ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg
    11821 gcacgactgt atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg
    11881 ccagtaccgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt tggcaatggc
    11941 gcggaagaat gcgccgtcag gctttgggtt tacagtctcc ggcctctacc gcagtagtcg
    12001 gtcgccaagc tttaaccgct atcatcgtat taggcgcaat ctggctgttg aatgattacc
    12061 gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt actcggcgga atgtttatgg
    12121 caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctg gaagcagcac
    12181 gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt aacggattaa
    12241 tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca ccttctgcgg
    12301 gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc agcctggctg
    12361 gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct tcactggata
    12421 acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa ggtgcgattc
    12481 tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt tgataaaaat
    12541 tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca gcaggaaaag
    12601 aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata aagcctatga
    12661 ccggcaggta gtagaaggcg taggggaata tttacaggca tcacaatcag aatgggatat
    12721 tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt taggagatgt
    12781 cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg tcgacgtccc
    12841 cattgttggg gttggcggct cgtatcacct tgcagaaagt tacccacccg ttcattacat
    12901 tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag agaaaggcgt
    12961 taaccgcttt gctttttatg gtcttccgga atcaagcggc aaacgttggg ccactgaacg
    13021 cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg tttatcaggg
    13081 gttagaaacc gcgccagaga actggcaaca cgcgcaaaat cggctggcag actgactaca
    13141 aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgagcgc ggcatattct
    13201 gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga ttggcatcga
    13261 taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc
    13321 gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata aagaagaaat
    13381 gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct caacagatta
    13441 tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta atcacgcctg
    13501 taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct ccaatcttga
    13561 gaagcgtttt aaagaagagg tgggtgaaac catccatgca atgattcatg ccgagaagct
    13621 ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga tatcgcaaat
    13681 gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat atgacacaac
    13741 gccaaaagag tatcgcgatg taaatagcga ggtcatgttg tagcgatgat gagaattgtc
    13801 ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct tatccgaccg
    13861 acaaatcaga taaggctcga ggaaatgaaa ttacatatgc gccgcgatta accgttggtt
    13921 atcctggtac attgcgaaca ggtagttgtt ataactcttc cctttggtcg agtagccctt
    13981 cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca gctgtgcacg
    14041 cgatttacgg aacgaagagt aagccgggtg cgtattcagg ttagtgacat aggcgctcac
    14101 cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg cattggtaca
    14161 acgtcctttc atgcatttca tgccgaacag attgttattg ttgcgcgcca gcttcgacgt
    14221 tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtaggga taatgtctac
    14281 gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact tcaccttgta
    14341 gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gcccattggc cctgatactg
    14401 ttttgaaatc agccagttac gttctgcagt aatggctgca ttttggctgg taatgtaagg
    14461 cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt ttcgcaaatc
    14521 aggaagtgaa ctactctttg cactattgcg agaatactct tgtttactgc ttacctgttt
    14581 attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa
    14641 cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat atcgtcgtat
    14701 gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc ttatattttt
    14761 cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag
    14821 gaatagtcca aatctgaaac tatgtcacga gttaacgatt cagattggcg ctaaatcgca
    14881 gaaaatgtgg gggttatcgc aaaattcagc cgttttttgc gcgcgatcgc tcacccttgc
    14941 ttctcatcct gtggacttac cgctcaggga tgagttttgt ctggcttatc gctggcaaac
    15001 tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct gttttctgac
    15061 actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc ctgcctttag
    15121 cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact
    15181 cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac tcaatcagat
    15241 gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca gggacggcaa
    15301 atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt ccatccagaa
    15361 cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg aaatgggatg gattaccgct
    15421 cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt attacagcca
    15481 acaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta ccagcaacgg
    15541 tttactcctg ctggaacggg cagaaactga cacatccgcc cctttcgact ggcataacgc
    15601 cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta atgaccagag
    15661 ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg gcgatttacg
    15721 cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt tatggataag
    15781 cgccccattt gagcaaattc acggctgggt cggcggtggt acaaaaggcg atttcccgca
    15841 ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca atatgggcaa
    15901 cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc gtattctctt
    15961 tgatgtcgtg atgaaccaca ccagctatgc cacgctggcg gatatgcagg agtatcagtt
    16021 tggcgcgtta tatctttctg gtgacgaagt gaaaaaaacg ctgggtgaac gctggagcga
    16081 ctggaaacct gccgccggac aaacctggca tagctttaac gattacatta atttcagcga
    16141 caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata tcggcgatta
    16201 cgacaatcct ggattcgatg atctcactat gtcgctggcc tttttgccgg atatcaaaac
    16261 cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg atactcacgc
    16321 taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa gtcagtgggt
    16381 ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg agttgcccgc
    16441 ctggcagcaa ctgaaaaccg aagccagctc cgcgcttcgc gaatggaaaa aaactaaccc
    16501 cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg gccacggcgt
    16561 gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg attatcagga
    16621 gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc agcaaatggc
    16681 ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata cccgtctgtt
    16741 ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg cggtacaaat
    16801 cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg
    16861 tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca acgtcgcgca
    16921 ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac
    16981 gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg ataaagtgct
    17041 ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt acctcgggaa
    17101 gccgccaacc aggataaaat gtcagcccta atcagcgttg cgggataaag caccgctcac
    17161 tcttcaacag accgatttgc accccagcaa atgtagcgtt attgttacct tccttgctac
    17221 agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat ttacccgcca
    17281 ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc ctggcgcgat
    17341 tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact tccagacgct
    17401 actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgtaact acgacggtcc
    17461 acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga agttgggttg
    17521 ggatatcgaa ccacagaata ttgcactaac aaacggcagc cagagcgcgt ttttctactt
    17581 atttaacctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct
    17641 ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt ttgtctctgc
    17701 gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg attttgagca
    17761 tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga atccaacagg
    17821 taatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc aacacggcat
    17881 tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct tcagtgaagg
    17941 gcgcccgcta tggaatccga atatcgtact gtgcatgagt ctttccaagc tggggctgcc
    18001 tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca tcaccaatat
    18061 gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt gtgaaatgat
    18121 taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt actaccagcg
    18181 tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaaaatcgct gcctgattca
    18241 taaaccggaa ggagccattt tcctctggct atggtttaag gatttgccca ttacgaccga
    18301 gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc acaacttctt
    18361 cccgggactg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga actacgtacc
    18421 agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag aaagagcctg
    18481 ggctgaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac atgatttctg
    18541 caatatattg aatttggaag aatttgtagg ccggataagg cgtttacgcc gcatctggca
    18601 taaacaaagc gcactattcc ggcattgaga aacgccggaa aacgtttcaa acaggctgct
    18661 gctgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc tttgtcgggc
    18721 aggcttcaac acaggccggc cccgtctccc gatgccagca cagatcgcac ttaatcgccc
    18781 tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc agcatacagc
    18841 ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg cccatgttcg cggcttatcg
    18901 cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag gctactggcg
    18961 tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct ggtgacaacg
    19021 cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac
    19081 cgatacattt cgtcgcatcc gcaataataa accggttcat gatcattcct ttgcactaaa
    19141 acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga aatgttaaag
    19201 ttttggcact tgtccaaagc gttatgtgat cgcgcccagg tcatccctga cagtaaatcc
    19261 cagttcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat ttttctctcc
    19321 cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca cccgcccatg
    19381 aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt ctctgtccat
    19441 cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat
    19501 ggtattgcgg gttaacggct ggatctcatg ctgatggttt tcccagtatg acttcacgta
    19561 gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga gcggcatatg
    19621 ctggccaata taggcacggg ttcgcagcat cccggttgtg ggttccagct tataaatcaa
    19681 aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg cgatgttcag
    19741 tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagacagcg ccttctgccc
    19801 gacggcaata aatttggtag tcaggcggta actccctgcg gcgggcgcgg tggtcacata
    19861 gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta aaccagccag
    19921 ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct caatcagcat
    19981 caacccgcga aacagactct ggcttccggc tggacgctct ttttcctgcg ccatctcgtt
    20041 ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg
    20101 tgccgtagtt cacgatctcg acagataatt tataaccact tgatttttat gtcttttgaa
    20161 attcatcaat cagattgctt tgttaaaaag tgatcgatat atttgaaatc tagttccgca
    20221 tattgaaatt ttaagccaaa aaagcgatca aaaaacaagg aagcctagga tgaaagtgac
    20281 atttgagcag ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg ttgacagcga
    20341 aacggctgac gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca
    20401 cggcgttaat cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga
    20461 tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc
    20521 gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca
    20581 cggtattggt ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg
    20641 ctggcaggcg gcggaaaaag gctatattgg catttgctgg accaactcc
//
LOCUS       DAJTYL010000070        20474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYL010000070 DAJTYL010000000
VERSION     DAJTYL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.070"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..4298)
                     /gene="mukB"
                     /locus_tag="OO572_002499"
     CDS             complement(<1..4298)
                     /gene="mukB"
                     /locus_tag="OO572_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6661468.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIA"
     gene            complement(4298..5002)
                     /gene="mukE"
                     /locus_tag="OO572_002500"
     CDS             complement(4298..5002)
                     /gene="mukE"
                     /locus_tag="OO572_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6661469.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4983..6305)
                     /gene="mukF"
                     /locus_tag="OO572_002501"
     CDS             complement(4983..6305)
                     /gene="mukF"
                     /locus_tag="OO572_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6661470.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6302..7087)
                     /gene="cmoM"
                     /locus_tag="OO572_002502"
     CDS             complement(6302..7087)
                     /gene="cmoM"
                     /locus_tag="OO572_002502"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6661471.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7223..8002
                     /gene="elyC"
                     /locus_tag="OO572_002503"
     CDS             7223..8002
                     /gene="elyC"
                     /locus_tag="OO572_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6661472.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7979..8872)
                     /locus_tag="OO572_002504"
     CDS             complement(7979..8872)
                     /locus_tag="OO572_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6661473.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9026..9772)
                     /gene="kdsB"
                     /locus_tag="OO572_002505"
     CDS             complement(9026..9772)
                     /gene="kdsB"
                     /locus_tag="OO572_002505"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6661474.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9769..9951)
                     /gene="ycaR"
                     /locus_tag="OO572_002506"
     CDS             complement(9769..9951)
                     /gene="ycaR"
                     /locus_tag="OO572_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6661475.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10003..11235)
                     /locus_tag="OO572_002507"
     CDS             complement(10003..11235)
                     /locus_tag="OO572_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6661476.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11272..12258)
                     /gene="lpxK"
                     /locus_tag="OO572_002508"
     CDS             complement(11272..12258)
                     /gene="lpxK"
                     /locus_tag="OO572_002508"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6661477.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12255..14003)
                     /gene="msbA"
                     /locus_tag="OO572_002509"
     CDS             complement(12255..14003)
                     /gene="msbA"
                     /locus_tag="OO572_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6661478.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14040..16299)
                     /locus_tag="OO572_002510"
                     /pseudo
     CDS             complement(14040..16299)
                     /locus_tag="OO572_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16506..16790)
                     /gene="ihfB"
                     /locus_tag="OO572_002511"
     CDS             complement(16506..16790)
                     /gene="ihfB"
                     /locus_tag="OO572_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6661479.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16950..18623)
                     /gene="rpsA"
                     /locus_tag="OO572_002512"
     CDS             complement(16950..18623)
                     /gene="rpsA"
                     /locus_tag="OO572_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6661480.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18734..19417)
                     /gene="cmk"
                     /locus_tag="OO572_002513"
     CDS             complement(18734..19417)
                     /gene="cmk"
                     /locus_tag="OO572_002513"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6661481.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19590..20354)
                     /gene="ycaL"
                     /locus_tag="OO572_002514"
     CDS             complement(19590..20354)
                     /gene="ycaL"
                     /locus_tag="OO572_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6661482.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4931 a         5674 c         4967 g         4902 t
ORIGIN      
        1 gcgatgatga gctgcatttg caaacgttca cacaattcaa acagcgtggc gatagaacga
       61 gcatccagtc gcgctgcttc atcgaggaac agcaggcggc aaggagagat atctttaccg
      121 cgcaggcggc gagattcatc ttcccagctt tgtaccacca tcaccagaat cgacataccg
      181 gtaccaatcg cctcaccggt cgacaatgca ccagactctg cgcgcaacca accatcggaa
      241 ccacggttaa cctcaacttc catttccaga tagttgcggt aatccagcag ttcttcgcca
      301 atggtctgcg gcgtgcgctg ccccatatca atctgcggat taagacgctg atacaatttc
      361 gccaacgctt ccgagaaggt cagacggttg ctgttaaaca gatcctgatg ctgctcgtgc
      421 tgttcagaga gcacatccag cagcatggcg tgcgtttcac gcacgttcac gttgagacgc
      481 acgctgttca cctgaccaaa cgatacgttc tgcaacccct ggttgagcat acggatacgg
      541 ttctgctcgc gctgaatggt tttgcgaatg atgttcgcca cgctgcggga actgatcgcc
      601 agtttctgtt cacgggaggt taattcttcg gtcagacggc taagttcaat ctccatctgt
      661 tcgatagctt ccaccggatc atcggtacga ataatatcct gacgaatacg ttcacgcaga
      721 tgctgataaa ccgccacgaa gaactgaatt ttacgctccg gacgtttcgg atcttccgac
      781 atgcgcagca cgtcgcgcag atgttcgtta tccgccaccg ccagacgcag cgcacctaac
      841 gccttatccg acatggaacg caaatcatca gcggagagat aagccagctc acgacggtgt
      901 aagcggcgct caacgccgtt atctttcacc atgcgcatca ccgcacacca gcccgctttg
      961 gcggtcacta cctgctcgcg catctcaaag taatcccgct ccagcttgcg cagtttgcgg
     1021 gtcaggttgt ccatctccgc ttcgcagaag gtaagcgctt tttccagttg attgcggcgt
     1081 gaacggttat tgctcagttg cgcatgcagc tcgtcacggc gaatacgcgc ccgctcttct
     1141 gccccgctat cagcacgcac gccgatatcc tgcaattcac gttgcagatc gttgagtagc
     1201 tcttttttgg tgtcgtaaga acttttcagc gaagccagca cctggttgta ctgactcaac
     1261 tgcgctgcgt gaccgcgcaa cgcttcgcga gcgcgggtac gctccgcttc cgcctgttcc
     1321 agacgttcac gcagtttttc gttgagatcg ctgttaccgc taagcatttc tgccgagtca
     1381 gaatagctaa agtgcgcacg acgctgcacc acttccgtca gggcaaacgc ttgctgacgg
     1441 gcatcgcgct gcatctgctg agagtacgcg taatcttctt ttaactgttc gaactgttcc
     1501 gggtcgctct gcaataccga aacgatcggt tccagtttcg ccagttgatt gccaaactgc
     1561 tgaacaaaac gcgcggcttc ctgggcttca tccagacgtt cgcggatttc atcgacgcga
     1621 tccgccaggc tgtcatcagc caacaggttg agacgcggca gaatgcggtt cagcgccgta
     1681 acgccttctt tcgcctgctc aaactgaata cgctgctgct ggttatcatt ttcatgatta
     1741 ctcaacgccc gctccagttc gactcgacgg ctgttcagtt gacggatttc tgcttccggg
     1801 tcagactcaa acgccaccgc cagatgactg ccgataaagc ggctgaacgc ctgatgcaga
     1861 cgctgggttt tctgtacatc aaaggagagc gtggcgaagc gttcggaaag cacttcacgc
     1921 tcggcatgga ggctttcaat acggctttca cgcgcagcac gaccaaacag cggcacttcc
     1981 gggaaacgtg aataacgcca ctgtcgatcg gcgattttca ccaccaccgc tttttccagc
     2041 tcatcaacgc tgaacacgct atcatcgaat gactgcggat ccccttcgat caaatagaga
     2101 tcttccgggc aatcggtcaa gccttccagg tgttcggtta cctgtgacag atctggcacc
     2161 acgatggcgt ggcgtgacgg gccatacaga gccgagaagt acggcgcatc ttccaggcta
     2221 acgtcgtcat aaatttctga cagcagcaca ccaccaaaac gctccgccag cgcgttcaga
     2281 cgctgatctt cagagccgcc aggctggctt aaacgttcga tctcttcatc gacggcgttt
     2341 ttgcgcgcgc ctacttcatc gcgttcaaca atcgcctcgc gctcacgctc cagcaactgt
     2401 tgcagatatt cagtgacgtc ctggctggag gagaactctt cgccgcactg ttcactcaac
     2461 tggttgagac tgttttgcgc tgccagccaa accggtgcac gctgcatcaa actctgaatg
     2521 cgagactgca gctgttcctg ttcctgccgc agtgccatcc gctcttcacg ggcgttagat
     2581 acgctatcgg aaagagaggc aatgcgtgct tccagctcct gatgcagagc ttccagttca
     2641 tcaatatcaa aattcttgcc ctgacgtttg cagaaatctg ccagcagacg ctcagcttct
     2701 tgctgctcac gcagacgctg ttccagttcg cttaagcgca tccgcaacgg ctgaacctgc
     2761 tctgccaggt gacgctgatc aaccccttcg cgcaatagtt cgcgagcgac atcccacgcc
     2821 tcgttacgcg ccagcgggcc gttgattgcc accaccagct gataagcctg ctcaaactgg
     2881 ctgtgcgcgg tttgcgccat gctcattttc tgctcaagag agagcatttt ttcagtcgct
     2941 tccagctctt tcgcctggaa ggtttccagc cattcggcgg cgctgtcggc ggttaagtcc
     3001 ggcagatggc acagttcttt ggcacgatta agcgcagcaa tcgcctggtt atactggatc
     3061 gcgcgcgttt gctggacgtc cagcgcctgc tggtagtcag caagctggct tttcagctcg
     3121 tccacttcca gttcggcagc ttccgcgcga gcctcattct cttcctggcg ttcgatggct
     3181 tctgccacca cttcattttg ctcttccaga cggatctgca gctcatcgag atccacttcg
     3241 tagcgttcga tcttctcctg ctgacgcaat gcggtttgca ccaggttcag gtgatcgctg
     3301 gccgcctgat aatccgcttc cagatcacct tcggcaccgt tgtgctctgc cagctcacgc
     3361 gccatatcga cgtgtttgta ctgctcagcc gccagttgct gacgcgaagt atgtagctcg
     3421 cgacgaaact ccagggcttt gtcgagatgg acacggcgct cgttggcgtg acgcatgtag
     3481 tccgccgcca cgtagttggt agcttcgctg atcagatgct taaacaggtc acggtccgac
     3541 tgggtgacac gaatcgcttc cagcgtcatg cggttttcac gcagcgccgc ttccatgtcc
     3601 tggaacgctt tacgcacgcc gctgttttcc ggcaacaggt agtcgcgcag agaacgggta
     3661 atggcactgg agatcccgcc atacagcgaa gcttcgatca gacgatagaa tttgctacgg
     3721 tcagatgcgg agcgcagacg acgcgcgatg atgcccagat cgaacatcag cgagtggtaa
     3781 tcggtaatgg agttgaactg tttaaactgc acgccttcca tcgcctcgag cttgtctttc
     3841 agctcgttaa gcggcagcac gcgcgcctgg cgttcgttca gggtttcggt caccagctgt
     3901 gtcggctgca ccgacatcgg cagtccctga atggcaaatg gtttgatatc cactttacga
     3961 tcgcgtccgg caacctgttg cagacgcaca ccgaccacca cgcgctggtg gcgcgagtta
     4021 atggtgtcga gcatcgaata acagacaccc gctttcagct taccgtgcag acctttatcg
     4081 cgcgaaccgc tggtggcccc ggcttccgta gtgttacgga aatgcagcag ggtcaggtcg
     4141 gggatcagcg ccgtaacgaa cgccgccatc gtggtggatt tacccgcccc gttaccgccg
     4201 gaaagcgtcg tgaccagctc gtcaaggtca aaagttcggg caaaaaagcc gttccagtta
     4261 atcagcgtca gtgagcgaaa tttaccgcgt tcaatcatta ttcttcctct ccgctatctg
     4321 gctgactctc ttcggtttca tcgttgagtt gcagatgatt ttcaatcggc attgcttcgc
     4381 catcacgaat caggcgacgc tgtgcttcac ggggatcgtc gccagcacgc acatcggctc
     4441 cgaagcggaa caccgattcg gtaatgcgga acttgctgct gtcgtggccc ataaaccaca
     4501 ccatgcctaa acgacgcaga cggttgagcg aagaacgtac tttctcctgc aacttctgac
     4561 ggtcaacgtc tgaaccggtt gaacggttgt tcaccagttt cagcagtttc gcttcatcgg
     4621 ccagggtgag cagttcgtcg tacagttcct gctgggtgaa aatcccctca ttcgccagcc
     4681 gttccgggct gagatagaga taacagagga ttttcccgac catcatatcc agttccgaca
     4741 agacggaacg cgggataaga gtggtggaac gtgggcgtaa atagaagaac ccttccggtg
     4801 cgcgaataag ctcaacgtta taacgcgcgt aaaactcttc cagatattcc tgaaaatcca
     4861 tcaggaatgc atgattatcc agttcgtcga ggccaatatg gcggcctgaa cgtaaggcgc
     4921 tgtccagcgc cggaaataac ggattcgcca gcgcctgcgc cagcttaacc ggcatcactt
     4981 gttcaatatt tgtcgatgac atgcgcctgt accttggctc cgtaatcatt aatcggctgc
     5041 catttcgctg gcagtccggt gaaatctgct tgcgctacgc caagacgtac cgcctgatca
     5101 ataacaatac gcgcaacgtc aaagtgacgt gcacgcggat actgtgacag atattcgcgt
     5161 accaccagac caagatccag cggcacttgt ctggttttgt acacggcaag ttgttcttcg
     5221 atgatcgccg ccagctgttc gcggatctcg ttaaactctt cgtattccag atcctcagga
     5281 agttccccag tcacttcttc atcgcgcagt gccatctctt cgtcacgcat atccagcaga
     5341 cgatcggcat tggcataagt tagcgcccac ggctcatcaa aataggtttg taccgactga
     5401 cgtaaccgct gagcaaagac gcggttttta tccatatcga tcgcggtacg aataaatttg
     5461 tgtacgtggc ggtcgtagcc aatccacaag tcgatggatt gctggcccca actgataata
     5521 cgatcgagtt tgctctgcag atcgaacacc agacgatcga cgaaatgcag atcgtcatgg
     5581 gtcatcgtcg catcctgaat gcgcaacaga ttagcctgca atttgtcgcc tgccgcttcc
     5641 agcgtatcct gcaattcacg cagcgttccg gaagtttccg aaagcaacaa ttcacagctg
     5701 gaaatcgccg cccgccagtc tttgttcagc aactgggcga tatcgtcctt cacctgctgc
     5761 tgctgttcgt ccatcagacg ttgcgtcagg tcgatactgt cgaaaatttc tgctaccgaa
     5821 tatttcagtg gcgcatagac attacggtgc cagtgaaatt catcaccgcc ctcttcggcg
     5881 gcatctgctg cgcgtttgag ctcacccgcc acaatcgaca actgcataga aagacgcagc
     5941 gtagaaaact cgcgctgacg gatgtagtag tcagtaatgc cgatgccgag cggcgtcaga
     6001 cggtaaattg cgttcccttc cgcctgctcg ctggtaaagc ggttcagcag acgttgacgc
     6061 accatgtcgt tgatcgcgtt attggcgcgc acgccgatgg tttcgctggt ttgctcaaac
     6121 gcatcactca catggcggaa tgcatccacc agctcgcctt cactcatctc accatccaga
     6181 cgctcgccgt tcagcgtggc aaccgccagc agaaaagaga gtcggtctac cggcagcgag
     6241 atggagaagt cattttttct ggcccaggca accagttcgg ggactgtctg ggaaaattca
     6301 ctcatacttt atccttgctc tgcggtttgc gcgcggtgac atgaatataa cgccctaggg
     6361 taatatacgg ttcctgacgg caataacgcg tttctaattc aagtaatgct tcatagcagt
     6421 cgcgctgctg gtacttctcg cgcagataat catgaaacac gcgaacgcct gtcttaccca
     6481 taatttgcca accagcttct tccagccaca gataaacctg cgccgggtcg cgtggataat
     6541 ctggcgaaag cgtccgtttt ttctttttcg gcattcccgc ctgcacgtaa tcaaaattcc
     6601 cggcgaccat gttatgcatc aacaaaccat gcgcattgta gaacattaac gacaacactc
     6661 cgcctggacg caacactgac cacagggtct gcaatacact gcggggatcg gccacccact
     6721 cgagcaccgc atggaacaat atcagatcaa cgggcgtttc caaatgcgaa gcaacatcct
     6781 gagcggcgca atgtataaat tgcatgttgt cgctcacacc ttttgcttct gccgcctgtt
     6841 ttgcgcggtc gatcatctgc gcagaaagat cgcataaaat aacctgatgc ccgcgctcgg
     6901 ccattttgat tgcggtctgc ccttctccgc cgccagcatc cagcacacgc agtttttgcg
     6961 ggcccatttc cgccagcacg cgatcgagat cctgccacag aatagcctgt cgaagctgcc
     7021 ctttggtggt gccgtaaatg ttacgggaaa acttttccgc aatatcatca aaattgcgat
     7081 cctgcattgc tttctccacc ggttggctaa gacgttttct gataaagacg ctattttgtc
     7141 acagccacgg cgagaatgaa cccgtctggt gtaatatcgc tagcgatccc ttgctatatg
     7201 gttaaaaaag gaaccagaaa ggatgctttt tacactgaaa aaagtgattg gcaatatgtt
     7261 gctgccactt ccgctgatgt tgttaattat tggcgctggc ctggcgctgc tgtggtttag
     7321 ccgttttcaa aaaaccggga agatttttat tagcattggg tggctggcac tcttgttatt
     7381 gagtttgcaa ccggtagccg accgcctgct gcgtcccatc gaaagcacct atccgacatg
     7441 gaataattcg caaaaagtgg actatatcgt ggtgctcgga ggtggttata cctggaaccc
     7501 gcagtgggca ccgagctcta atttaatcaa taacagtctg ccacgcctga atgagggtat
     7561 ccgtttatgg cgcgaaaatc cgggttcaaa actgatcttc acgggaggcg tagcaaaaac
     7621 caatacggtg agtacagcgg aagtaggtgc cagagttgcg caatcgctgg gcgtaccgcg
     7681 cgagcaaatt atcaccctgg atttgccaaa agataccgaa gaagaagctg cggcagtgaa
     7741 acaggcgatt ggtgacgccc ctttcctgct ggtgacctcc gcatcacatc tgccgcgggc
     7801 gatgatcttt tttcagcagg aagggttaaa tccgctacca gccccggcga atcagttggc
     7861 catcgactcg ccgcttaatc cgtgggaacg ggcaatccct tctccggtct ggctgatgca
     7921 tagcgatcgt gttggctatg aaacgcttgg ccgcatctgg cagtggctga aaggtccgtc
     7981 aggcgagcca cggcaagagt gattttgatg ccagatcgaa gttgcgccga ctaaatcgtc
     8041 cggtattaac cagttgcgca acttcatccc ataacacata caaccaacgc cgccagatga
     8101 acgactccgc cactggcgcg cgttgcaggt aactccagag caaatcttcc gccagagaat
     8161 tatccattag tcggaacagt tcgtattctc gcggtgccca aagcattagt cccggcccga
     8221 ccatcgccag taactgatcg ctgcgcgaat ctttcagcat gctgcgtaaa cagaagttac
     8281 cgtgaatcaa cacacaattg tcgttaaagc cttcaaataa tgccgggaga cattcgcgag
     8341 tgcgaaacag gatccgctta tcctgcatcg tcagaccggt gttattgaac tgattgagcg
     8401 tggtccatag cacttcaaca tgttgccggt accatgaggg ccagaaattt tcctgagtat
     8461 tgtcgaccgc gccgacgcaa ccgcgactgt cctgacggtg ccaggccagt aaggcttcaa
     8521 cgatttggtc tttgagctgt tcccagcgtt ctggtgttcg ggctggtgcc tccaccgaaa
     8581 caccacgcat ccgctccagt agcaggacat cagggccggg gtgttcttca tgcgtcatca
     8641 caccataaat agtcggcata cggacagtcc cacttcttgc cagcatggtg gttttccatg
     8701 ccagttgtcg ggcttttccg ggcgtactaa agcttcttgc cattaacggc attgggtttc
     8761 cctggctgtc atacaaagcc cacaacgccg tatccgcttt ttcattgacg cactcaatac
     8821 gactgagttt ttcgcccagt aaatggctta attcggcacg cagctgttcc attcagatta
     8881 ccctcataaa agctactgct ttcataatga gcgccaatat aaaagatgtc acctgaatga
     8941 gatcaattgt tgaaaagaaa aattgaggaa aatcgtaacg gtacgacact cctcccaaaa
     9001 ttggctgaag tgtcgtgaag tgaaattagc gcatttcagc gcgaacgcgc tcaagatctt
     9061 caggggtatc cacacctgtg ccaggaactt cctgagcaac agcaacatgg attttttcgc
     9121 cgtaccacag aacacgaagc tgctctaaca tttcgatgtg ttctaacgga cttgcctgcc
     9181 agttgacgta acgacggata aagcctgcac ggtagccata aataccaaga tgacgcagga
     9241 agttatcgcc aacggtttca aggccttctg caaaacgatc acgatcccaa ggaatggtgg
     9301 cacgtgagaa gtacagtgca tacccttcag cgtcgagaac cactttcacc gcattcgggt
     9361 taaacgcttc ttccgcattg tggattggca ccgccagagt cgccataccc acctgacgct
     9421 gagcgaggtt atcagcaacc tgacgaatga ttgtcgcagg gatcatcggt tcatcaccct
     9481 gcacattaac gatcaccgtg tcgtcgctga atgcgcattt ttcgacaact tccgccagac
     9541 gttctgttcc tgactgatga tcggcgcgcg tcatacatac ttcaccgcca gcggcttcaa
     9601 cggcgcgggc aacatcatca tgatcggttg ccacgatgat gcgctcggca cctgattcac
     9661 gcgcgcgttc aagaacatga acaatcatgg gtttgccgtt aatatcaacc aatggtttac
     9721 cgggcagacg cgtggacgcg tagcgcgcgg gaataatgac cacaaaactc atgatttact
     9781 ctcatcagca gtcagcacgc gggcttccgt ttccagcaac actggaatgc catcgcggag
     9841 cgggaaagcc aggttatcca gtttgcaaat aagctcttgt ttttcctggt tataccaaag
     9901 ttttccgttg caaaccgggc aggcaatgat ttcaagcaaa cgatgatcca tagttcctcc
     9961 agatggatcg ggttatgaat gcataaatct tatcataatc atttatgcga cggggtctat
    10021 ttcccagcca gtgcggcaat cagtaaagag accttgcggg cagcgtccta atgtcacccg
    10081 cgttgcctgc tgccagctcg cgaaatcagt aatcgcctga cgtaaccctt tttgcagcat
    10141 tgtcgttggt ttaatgcctt cctgtaacca cagagagata acttcaagga tgcctgtctg
    10201 gcgatgcatt ttggcatcca ttcgcccaac taattgccca cgatgtaata atggcagaac
    10261 aaaatagcca tactggcgtt tcggcgctgg ggtatagcac tccagccggt agctaaaatc
    10321 aaaaagctgc tctgcgcgtt tgcgatccca gacaacagga tcaaaaggcg aaagtaccgc
    10381 gctgtgcgtt gcagtgagct ttccggccag cgcacgctcg agtagcggca gcaaatcagc
    10441 atgcagccaa agattgccca atttttcaac atgcacagca atgatttgct gctgttcagc
    10501 cctcgcttcg cgccacgccg ccagtgccgg acgtttcagc cgatagtaat ctgccagcca
    10561 ctgttcgcgg aatattccca ggctacgcgc actgttatcc agcatgatga tttctgcttc
    10621 tgtttgcgaa acgagatcgc gctcatcatc ccagtcaggc atgacacggt gggttaaatc
    10681 ataaacgcgc tggaagttgc gccgttcaat caccatcacc tttccggcag taaataaacc
    10741 ttccagatgc cgtttatgcg gcttccattc ccaccagccg cttgcacctt tacgaggatg
    10801 ctcaaaatcg gctgaacgta ccggcccttt atcatgaata tgctgaatta actgtgcaat
    10861 ttccgcctca tgttcctgca tccaggcgtc tttgtatttc cagcccattt tttcaggtgc
    10921 cagcatgcgg tggcgaataa gacgaaagtc gctacgcggc ataaagcagg cttcatgcgc
    10981 ccagtattcc attaattcgc cacgcgccag agactcatcc agccactggg caggataatt
    11041 tcccagacga ctgaaaagca ccagatatgg actacgggca acaatattga tggtatcgat
    11101 ttgcagcaag gacatgcggg agatcgttgc cggaatatcc tccaacgacg ctcgacggcg
    11161 gggtttgttt aacaggcctt gtgcggcaag gtgaagatta cgcgcatcag caagggagag
    11221 gtgcggcagc gacattcatg actccatcaa tcgaacgctg ccgcggcgta actagttgcc
    11281 agaagccagc gaggttagtt gcgtaagcag tttcgctggt tcatcacctg aaagctgtgc
    11341 gtctacaggc aaataccacc aattttcttc tgcaaaggcc cggcatttca ccgcatcttt
    11401 ttcagtcatt accagcgttt gcccggcgct taccaacgca ctgacatcct catggttcaa
    11461 agactgatga tcggccagcg gtacacattt ttccggttgt acgccacaca tcttcagcgt
    11521 ggcaaaaaag cgcggcggat gcccaatccc cgccatcgcc actacatgtt caagctgagc
    11581 aacgtcacaa cgcgtaccgg tacgtaaatt caccgcctga cccggcagca gatgcatggg
    11641 gatttcaccg ctgcgaggga caccgccgtt gacgattacc gcatcaaccg actttaagcg
    11701 ccccgctcgc tcacgcattg gccccgccgg caaccaccag ccattgccaa agcgacgcac
    11761 accatcaata acgacaattt ccacatcacg cgccagacgg taatgctgta aaccgtcgtc
    11821 ggttacgatg atctgcacat cagggtgttg cgccagaatg gcttttaccg catcagaacg
    11881 aacgggagaa accgcaacag gcgcatcagt gcgttgataa atcaacacag gttcatcacc
    11941 cgcctgtgct gtggtggtat ctgccgacaa taacagcgga taagattcag ccttaccacc
    12001 atatccccgc gatacgaccc ccacgcgaat accgcgctgt tgcaactgtt ccaccagcca
    12061 gacaacgacc ggggtttttc cgttgccgcc tgcggtgaga ttaccaacca cgacaaccgg
    12121 tacgggggca cgccaggcgc gcttcagttt tagtttatag caaagacgga tcgcgccact
    12181 caccaggcca tacaaccagg agagtggcag caatagccgc cacaaagggg attcaccaga
    12241 ccagattttt tcgatcattg gccaaactgc attttgtgaa gttgcgcgta aacgccgcgg
    12301 tgctcaagca aatcgttatg cgtaccgcgt tccacaatga caccatcctc gacgaccacg
    12361 atttcgtctg ccttttcaat ggtagacaag cggtgggcaa tcaccagaga ggtacggttt
    12421 ttctgcaact catccagtgc cgcctgaatc gcacgttcgg attcggtatc cagagccgag
    12481 gtagcttcat ccagaatcag aatcgggcta tcacgcagca aggctcgagc gatagcaata
    12541 cgctgacgct gaccgccaga gagcagcacg ccgttttcac caatcactgt atcgagaccg
    12601 ttatccatct tattgatgaa gtccatggcg taggccatcc gcgccgcttc ttcaatttgc
    12661 tcacggctgt actgttcagt ccgtgcgtaa gcaatgttgt tagcaaccgt atcgttaaac
    12721 agatggacat tctgcgacac cagagcaacc tggttacgta acgacgccag ggtatactcg
    12781 cgcagatcgt gaccatccat caggatttcg ccttcatcaa tatcgtaaaa acgcgtgatc
    12841 aggctggcga tggttgattt acctgaacca gagcgtccaa ccagagcaac cgtcttccct
    12901 gccggaattt tcaggttgat gttacgcaat gcgggtacgt cacgtcccgg ataagtaaag
    12961 gtgacattgc ggaattccac gtcgccagtc gcacgctcga tcacgcgctt accttcgtct
    13021 ttctcctgct cactgtccag aatggtaaac agcgtctgac aagccgccat accgcgctgg
    13081 aactgggcgt taacgttggt cagcgatttc agcggacgca tcagtgcaat cattgaagag
    13141 aaaacaacgg taatcgtacc ggcagtcagg ttatccataa cacttgggaa gctcgccgca
    13201 tacagaacaa acgccagcgc caaagaggcg atcagctgaa tgatcggatc agagatggaa
    13261 gaggctgaaa ccattttcat cccctgaaga cgcattcggt tactgacttt atcgaagcgt
    13321 ttcgtttcca cttcctgacc accgaaaatc aatacttctt tgtggccctt cagcatttgt
    13381 tctgcactgg tggtcacttg ccccatggtg ttctgcatgt ttttactgat gttgcgaaaa
    13441 cgcttcgata ctacgcgaat cgcaatcgaa acaatcggtg ccagcacaat caaaatgatc
    13501 gacagttgcc aactgtaata gaacatcatg atgaacaggc cgatgatcga cgcaccttca
    13561 cgcacaacag taatcagtgc gccggaagaa gaagaagcaa cctgttcgga atcgtaggtg
    13621 atacgtgaca acagcgtacc cgttgactgt ttgtcaaaga atgaaactgg cattcccatc
    13681 atgtgaccaa acaggcggcg acgcatggtc attaccacct ttcctgatac ccaggagata
    13741 cagtagctgg agacatagct ggtgatacca cgtaaaatca tcagtccgat caccaccagc
    13801 ggcatccaca ccagcacgga gcgatctgtt ttaccaaagc catcatcaag aagtggctta
    13861 aggagcgata acatgaaggt atcgctggct gcgttgagga ttaacgctac gcccgccacg
    13921 atcagacccg ctttgaaagg cgcaatggtt ggccacagtc ggcggaatgt ctgccacgta
    13981 gagagatctt tgtcgttatg cattcaaaaa accagcattt gttgaaatag ccgcatattc
    14041 tacccgttat ccactggcac gccaaaccac tgatgatacc aacgaggtaa aatttgctct
    14101 ctgaggctgc taatccgcca gccttgtgct gaaaaattga ccgtcacttg cccctgatgt
    14161 ggagtatcaa gccattgata tcctttctgt tgatagcgat gcttaacttt gttagagggc
    14221 aatcgccatg cgttatagcg cgatgccgat gcgagtgcca cttttccatt cactcgctga
    14281 attaatggca acgatgatga ggtattactg ccatggtgag gtacctgaag caatgttgcc
    14341 tgcacttgct gccagtaacg gcttagcatc ttttgttcag ctggggcttc aatatcaccg
    14401 gttagaagaa tgctattcgt cccgtcatca accttaacca cacaggaatg gttatttccc
    14461 ttatcgttgc taccttgtaa aggccagtgc gcgctgaaac gcaatccttg ccactgccac
    14521 gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct gatccataac
    14581 atcggccata tatgcaatat tgaatccagc cctccccggt gatccagatg ttcatggctc
    14641 agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat cagttgttgc
    14701 ccactatccc cttcaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    14761 caccattgcc agcccttgcc cgacatcaag catgtacagc tgccactcgt caggtcgagg
    14821 tttttgccac agcggccagc acatcagcaa gcctccagcc acacacattg ctggcaacgt
    14881 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    14941 ccaacattca acaatgttga tccacccttc cggcaaactc tttaaccccc agaaaagtaa
    15001 agccaaagac cggtcggcaa gaaaccataa cccctgttcc aggattaacg gcccgctaaa
    15061 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15121 aaatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcatggg
    15181 cataagcagg agtgtgattc ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15241 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15301 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15361 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15421 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15481 ccaggcataa aaagcagcac agcagattcc gccaattaat ggtatttgcc agtggatcca
    15541 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acgccgcaaa
    15601 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15661 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15721 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    15781 attcggttca atgacacttg cctgcagaaa acggccggtg agcggctgat gctgggcaat
    15841 ggcataacgc tggctatcaa agccgccatc attaagttgg ccatgaactg cacgaacttt
    15901 gagtttcatt gaccattgtt gtccagcaca aaccgcttgc ggaagttatt cgccatacag
    15961 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16021 agtggtcatg ccgtcagttg cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16081 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16141 cgtcagcgcg atataacgga cggttttaac agggataaat gccagtacgc aggcgaagag
    16201 agtcagaaac gcaagggtta atgtcctagg caattggggc aaaatcagca acggaaaaat
    16261 tccgcaaatt atgcatacgc cgactgtcgt tattttcatg cacccctccc tgtttatgga
    16321 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16381 cagaagaatt taacgaaagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16441 ctgattagag aattgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16501 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16561 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16621 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16681 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16741 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    16801 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    16861 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    16921 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    16981 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17041 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17101 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccagggt agcgtcttca
    17161 acgcggtcac gggatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17221 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17281 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17341 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17401 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17461 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17521 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17581 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17641 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17701 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    17761 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    17821 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    17881 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    17941 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18001 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18061 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18121 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18181 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18241 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18301 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18361 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18421 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18481 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18541 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18601 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18661 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18721 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    18781 tcaattgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct gccggaacca
    18841 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    18901 gctcgaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    18961 cttccgagga ggcgtcaagg aaaattttca ctggtgcatc agggaatacc accgttccca
    19021 tgtcgcggcc atcggcaatc agacctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19081 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc cgcattcgcc acttcctgag
    19141 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggtcg
    19201 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttcc gacgcaacat
    19261 caacatggtg atgtaatgcc gccaatgcca gtacgcgata aattgcaccc gagtccagca
    19321 gatgccattg caacgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19381 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19441 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19501 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19561 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19621 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19681 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19741 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    19801 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    19861 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    19921 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    19981 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20041 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20101 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20161 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20221 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20281 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20341 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20401 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20461 cgccgacaat gtgc
//
LOCUS       DAJTYL010000071        20425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYL010000071 DAJTYL010000000
VERSION     DAJTYL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.071"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            158..499
                     /gene="raiA"
                     /locus_tag="OO572_002515"
     CDS             158..499
                     /gene="raiA"
                     /locus_tag="OO572_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6661483.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    602..727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            603..650
                     /gene="pheL"
                     /locus_tag="OO572_002516"
     CDS             603..650
                     /gene="pheL"
                     /locus_tag="OO572_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6661484.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            749..1909
                     /gene="pheA"
                     /locus_tag="OO572_002517"
     CDS             749..1909
                     /gene="pheA"
                     /locus_tag="OO572_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6661485.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1952..3073)
                     /gene="tyrA"
                     /locus_tag="OO572_002518"
     CDS             complement(1952..3073)
                     /gene="tyrA"
                     /locus_tag="OO572_002518"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6661486.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3084..4154)
                     /gene="aroF"
                     /locus_tag="OO572_002519"
     CDS             complement(3084..4154)
                     /gene="aroF"
                     /locus_tag="OO572_002519"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6661487.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4364..4729
                     /locus_tag="OO572_002520"
     CDS             4364..4729
                     /locus_tag="OO572_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6661488.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4879..5397
                     /locus_tag="OO572_002521"
     CDS             4879..5397
                     /locus_tag="OO572_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6661489.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5387..6612
                     /gene="dgcN"
                     /locus_tag="OO572_002522"
                     /pseudo
     CDS             5387..6612
                     /gene="dgcN"
                     /locus_tag="OO572_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6628..7110
                     /locus_tag="OO572_002523"
     CDS             6628..7110
                     /locus_tag="OO572_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6661490.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7187..7534)
                     /gene="rplS"
                     /locus_tag="OO572_002524"
     CDS             complement(7187..7534)
                     /gene="rplS"
                     /locus_tag="OO572_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6661491.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7576..8343)
                     /gene="trmD"
                     /locus_tag="OO572_002525"
     CDS             complement(7576..8343)
                     /gene="trmD"
                     /locus_tag="OO572_002525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6661492.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8374..8922)
                     /gene="rimM"
                     /locus_tag="OO572_002526"
     CDS             complement(8374..8922)
                     /gene="rimM"
                     /locus_tag="OO572_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6661493.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8941..9189)
                     /gene="rpsP"
                     /locus_tag="OO572_002527"
     CDS             complement(8941..9189)
                     /gene="rpsP"
                     /locus_tag="OO572_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6661494.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9326..10687)
                     /gene="ffh"
                     /locus_tag="OO572_002528"
     CDS             complement(9326..10687)
                     /gene="ffh"
                     /locus_tag="OO572_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6661495.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10854..11645
                     /locus_tag="OO572_002529"
     CDS             10854..11645
                     /locus_tag="OO572_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6661496.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11666..12952
                     /locus_tag="OO572_002530"
     CDS             11666..12952
                     /locus_tag="OO572_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6661497.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13007..13600)
                     /gene="grpE"
                     /locus_tag="OO572_002531"
     CDS             complement(13007..13600)
                     /gene="grpE"
                     /locus_tag="OO572_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6661498.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13723..14601
                     /gene="nadK"
                     /locus_tag="OO572_002532"
     CDS             13723..14601
                     /gene="nadK"
                     /locus_tag="OO572_002532"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6661499.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14687..16348
                     /gene="recN"
                     /locus_tag="OO572_002533"
     CDS             14687..16348
                     /gene="recN"
                     /locus_tag="OO572_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6661500.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16497..16838
                     /gene="bamE"
                     /locus_tag="OO572_002534"
     CDS             16497..16838
                     /gene="bamE"
                     /locus_tag="OO572_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6661501.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16900..17190)
                     /locus_tag="OO572_002535"
     CDS             complement(16900..17190)
                     /locus_tag="OO572_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6661502.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17180..17617)
                     /gene="ratA"
                     /locus_tag="OO572_002536"
     CDS             complement(17180..17617)
                     /gene="ratA"
                     /locus_tag="OO572_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6661503.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17788..18270
                     /gene="smpB"
                     /locus_tag="OO572_002537"
     CDS             17788..18270
                     /gene="smpB"
                     /locus_tag="OO572_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6661504.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18485..18847
                     /gene="ssrA"
                     /locus_tag="OO572_002538"
     tmRNA           18485..18847
                     /gene="ssrA"
                     /locus_tag="OO572_002538"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19052..20293
                     /locus_tag="OO572_002539"
     CDS             19052..20293
                     /locus_tag="OO572_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6661505.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5279 a         5338 c         4796 g         5012 t
ORIGIN      
        1 agatattcct caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg
       61 acaaaaagtg acaaaattat gagatttcca tcacacattt tgacatcagg aacggtatgc
      121 tgaattcacc aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa
      181 acaaatggaa attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga
      241 aaaatggcaa acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt
      301 tgttgctgac gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga
      361 agatatgtac accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact
      421 gcagcacaaa ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga
      481 agaagttgaa gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg
      541 ttttttgttg acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa
      601 acatgaaaca cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg
      661 tttcgtcgtg tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct
      721 tttttattga taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg
      781 agagaaaatc agcgcgctgg atgaaaaatt attagcgtta ctggcagaac ggcgcgaact
      841 ggccgtcgag gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg
      901 tgaacgcgat ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca
      961 ttacattact cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt
     1021 gctccaacaa catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc
     1081 caaaggttct tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt
     1141 cattgaaagt ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc
     1201 cgactatgcc gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga
     1261 tctgctgcaa cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg
     1321 tttgttggtc tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca
     1381 gccattccag caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac
     1441 cgaaagtacg tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc
     1501 gttgggaagc gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc
     1561 aaatcagcga caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc
     1621 tgatcaggtt ccggcgaaaa ccacgttgtt aatggcgacc gggcaacaag ccggtgcgtt
     1681 ggttgaagcg ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggaatcacg
     1741 cccgattcac ggtaatccat gggaagagat gttctatctg gatattcagg ctaatcttga
     1801 atcagcggaa atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt
     1861 attgggctgt tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc
     1921 cggatgatgt gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca
     1981 ataacacgcg gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct
     2041 tgcggaaact gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc
     2101 cgaaacgctt atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt
     2161 cggcataaag ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt
     2221 aaatcggcga agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca
     2281 gcccgtaagc aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt
     2341 gctcgacagc gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc
     2401 attggtatgc ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac
     2461 cgctgtccgg gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca
     2521 tggcctgtaa tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta
     2581 aaggcggtaa tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca
     2641 ttccggcatc ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca
     2701 cctgataacc cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc
     2761 cgacgataac caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac
     2821 tggagtaaga ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac
     2881 ccagagcttc cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat
     2941 aaataggcag tccaaagcgg cttttcacct tgcccacttc agcaaccagt tccagacgct
     3001 tcgctaataa attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca
     3061 attcagcaac cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc
     3121 tgatgaattt cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg
     3181 gatacaccgt atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata
     3241 ttactttcga tcatcagacc aataattgag cgattaccat ctttgatttg agcaaccacg
     3301 gattctgcca ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct
     3361 accatcagag acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc
     3421 gcagggctat agttcggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc
     3481 tgagtttgta gcaacgcaac ctgccctgcc tggttaatgc caacaaaaca gtgcggctgg
     3541 gcggcggcgc gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa
     3601 ccaaccggca tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga
     3661 gcaccaattg ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac
     3721 gcttccgtcg ccagtggcag tcccatattc accagctcaa gcaacaattt acgcgcgatt
     3781 tgcagcccgg cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc
     3841 cagccgacag tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg
     3901 ctgacctctg cggcaagagc tttaaatcga cgagcatatt ccagagcagt ttccggatca
     3961 tgaatggaac aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct
     4021 gaaatggttt tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg
     4081 gccttcagtt gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc
     4141 gcgtctttat gcatgatggc gatcctgttt atgctcgttt gcgatagttg ctcctcagcg
     4201 aggatgacgt aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta
     4261 tttacacagg caatttagtc gcgctttcaa cccttgcttc tgtatagata aatttacatc
     4321 ccctttaaaa acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat
     4381 cgcccccttg ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc
     4441 aaccttgacc agcaccgact ggtatgatgt cggtatggaa gatgcgatat cgggcagcgc
     4501 cataaaagat gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg
     4561 atatgccgaa gggcaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc
     4621 cggtaaaagc tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt
     4681 atggcaaaaa agggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca
     4741 caaataaatt aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacggtc
     4801 gcatgaaaaa atgatatttt aatgagcatc ctgaaaaaat atcgatgcat ttcgagcgaa
     4861 gatggtgggg atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg
     4921 ctgacgggag caccaatact cgctcaagag ccttctgacg ttgcgaaaaa cgtgcgtatg
     4981 atggtttccg gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta
     5041 tgtatctttt catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg
     5101 ccttatctgc ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt
     5161 ttttattttg gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca
     5221 aaggcgttat tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt
     5281 ctgatcatcc acaataatga cgtcagattt gccgtaaacc tggatgcctc atcgcgtagc
     5341 ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga
     5401 taattctctt aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag
     5461 catatttatc actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct
     5521 gaaacagtac gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga
     5581 agcagctgtc gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca
     5641 gcaagggcat tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg
     5701 cattaccccc gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc
     5761 cctgccccca tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta
     5821 accgctcgcg acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt
     5881 tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg
     5941 gtggaagcac tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt
     6001 tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     6061 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt
     6121 ctgcgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     6181 ggcatcaata cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt
     6241 gatggcgata atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc
     6301 ttgattgaaa tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc
     6361 ctgggcggcg atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcag
     6421 cagatatgct cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat
     6481 cagaccacca tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa
     6541 aaattacaag agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag
     6601 ctggtgagat aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc
     6661 acctcactaa tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc
     6721 gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca
     6781 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc
     6841 caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac
     6901 accgataact atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc
     6961 gtggccgatg catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt
     7021 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc
     7081 cgcgtcgcag tggtgattgc aaccccttaa aacacgagca aacagacaaa aaaagggcca
     7141 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct
     7201 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     7261 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     7321 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     7381 tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     7441 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     7501 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     7561 tgatctctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     7621 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     7681 aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     7741 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt
     7801 atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg
     7861 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     7921 accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac
     7981 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     8041 ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     8101 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     8161 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt
     8221 gaagtcgcga ggactccaac tctggatgct cagcaggcca tttttaactg cccggccagt
     8281 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca
     8341 catagcgccg ccttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc
     8401 gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag
     8461 acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga
     8521 tccggtttcc atcatatcaa cgactttacc gagatcgtag ccttcagtgg ttactacctg
     8581 gcagcccatc aggtctttcc agtagtagtc cccctcttca agctgaggca gctgcgatga
     8641 atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt
     8701 cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca
     8761 ctgacccgcc ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt
     8821 ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac
     8881 gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga
     8941 ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc
     9001 ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct
     9061 agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct
     9121 gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat
     9181 agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc
     9241 cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt
     9301 taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcattccct
     9361 tcatgcttct catcatcttc gccattccgc ctttcttcat tttcttcatc atgcgctgca
     9421 tgtcgtcgaa ctgcttcaga aggcggttaa cgtcctgtac ctgcatcccg caaccggcgg
     9481 cgatacgacg cttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca
     9541 ttgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc gacttgacgt
     9601 tatccgggat ctgccccatg cccggcagtt tgcccatcaa gctggccatg ccgcccatat
     9661 ttttcatctg gcgcagttgc tcaagaaagt cgttgagatc gaagccgtcg ccttttttta
     9721 gcttgctggc taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca
     9781 gcgacagtac gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct
     9841 ccagcgcctc agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac
     9901 gaatagagag cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg
     9961 taagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg
    10021 cgtcaaccac aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt
    10081 ccatcatcgc ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga
    10141 atttcagttt ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat
    10201 cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg
    10261 ccgggcgata gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga
    10321 aattaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca
    10381 cgaccgcagg cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa
    10441 ccagttcgtt acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt
    10501 catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga
    10561 cgtccgcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag
    10621 tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat
    10681 caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt
    10741 attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt
    10801 cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatt actatgcccg
    10861 tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc
    10921 tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg
    10981 tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc
    11041 tcagcctgct gaacgttggt tcattggtca gtttggtgat ctgtacggta atgaccattg
    11101 tggcttctcg caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca
    11161 acctggcgct ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg
    11221 ggatgctgat gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc
    11281 tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta
    11341 accaagaaat gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg
    11401 gcgtggtgct gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta
    11461 gcatggaaaa tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc
    11521 tgctgtgggg acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg
    11581 cgggcgcggt aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca
    11641 gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat
    11701 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
    11761 cctcaaccgc tatcgtcttc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt
    11821 cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct
    11881 ggtcaatatt cttgcctccg cgctcggcac tattgtcggg atgcgtttgt acggcgatgc
    11941 gggcgtggca attgcgactg gtgtgctgac ttttgtcgta ctggtttttg ctgaggtatt
    12001 gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
    12061 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat
    12121 gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga
    12181 agagttgcgc actatcgtgc atgaatcgcg ctcacaaatt tcccgtcgca atcaggatat
    12241 gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg taccgcgcag
    12301 tgaaattatc ggtattgata tcaacgatga ctggaaatct attctgcgcc aactctccca
    12361 ctcacctcac gggcgcatcg tgctttaccg tgattcgctg gacgacgcca tcagtatgct
    12421 gcgagtacgt gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat
    12481 gctgcgcgcc gcggacgaga tctattttgt gcctgaaggt acgccgctca gcacgcagtt
    12541 ggtaaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
    12601 tcaggggctg gtgacggttg aagatattct ggaagagatt gttggcgatt tcactacgtc
    12661 gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga
    12721 tggcaccgcc aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga
    12781 tgcccgcacg gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac
    12841 ccgcgtgcgt attggcaagt acgatatcga tattctcgac gtacaggaca atatgattaa
    12901 gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg
    12961 gccctgcatt cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt
    13021 tcgctacagt taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca
    13081 taatgcccag tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat
    13141 gcacattcgg gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca
    13201 caacatccag catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg
    13261 ggttagcttt atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt
    13321 cgttgatgaa tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac
    13381 gacgcaggtt ttccatttcg gcttttacgc gcaaaatacc gtcacgttca cgggtctggg
    13441 cttcagccag ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag
    13501 cagaagcttc tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg
    13561 gggcttgccc ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt
    13621 cgcattcatc tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc
    13681 actcacattg tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag
    13741 tgtattggca ttgtgggaca cccacggcac cctactgcac tgacaacaca tgaaatgctc
    13801 taccgctggc tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa
    13861 ctgcaactga agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc
    13921 gcggtagtcg ttggtggcga cggtaatatg ctgggcgcgg cgcgcacgct cgcccgttac
    13981 gatattaaag ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc
    14041 gataacgccc agcaacaatt agccgatgtg ctggaaggtc actacatcag cgagaaacgt
    14101 tttttgctgg aagcgcaagt ctgtcagcaa gattgccaaa aacgcatcag caccgcgatt
    14161 aacgaagtgg tacttcatcc tggcaaagtg gcgcatatga tagagttcga agtgtatatc
    14221 gacgagatct ttgcgttttc tcagcgatct gatgggctaa ttatttcgac gccaacaggc
    14281 tccaccgcct attccctctc tgcgggcggt cctattctga ccccctctct ggatgcgatt
    14341 accctggtgc ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc
    14401 agcacgatcc gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc
    14461 cagatagcac tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat
    14521 ctgaatctga ttcatccgaa agattacagt tatttcaata cattaagcac caagctcggc
    14581 tggtcaaaaa aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta
    14641 tactgtacac aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact
    14701 gaccatcagc aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac
    14761 cgtaataact ggcgagaccg gtgcgggtaa atctattgca atagatgccc tcggtctttg
    14821 tctcggtggt cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg
    14881 cgcccgtttt tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct
    14941 tgaagacggg catgaatgtt tgcttcgtcg cgtgatcagc agtgatggtc gctcccgtgg
    15001 tttcatcaac ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat
    15061 tcagatccat ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct
    15121 gcttgatggc tatgccaatg aaacctctct acttcaggaa atgaccgcac gttatcagtt
    15181 gtggcatcaa agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc
    15241 ccgtgcggaa ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg
    15301 agagtttgag caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac
    15361 caccagccag aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca
    15421 gctttacacg gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg
    15481 cgtacttgat atgctggaag aagctaccat ccagattgct gaagccagcg acgaactgcg
    15541 ccactactgc gatcgtctgg atctcgatcc caaccgactg tttgaacttg aacagcgcat
    15601 ctcaaaacag atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat taccacagta
    15661 ttaccagtcg ctactggaag aacagcagca actggacgat caggccgact cacaagaaac
    15721 gctcgcgctg gcggtaacga aacatcatca gcaggcactg gaaaccgcgc gcgcattaca
    15781 ccaacaacgc cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc
    15841 gctctcaatg ccgcatgggc agtttacgat tgatgtcaaa tatgacgagc atcacctggg
    15901 cgctgacggt gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca
    15961 gcctattgcc aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caatccaggt
    16021 catcacggcg cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat
    16081 tagcggtcca acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca
    16141 ggtgatgtgt gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt
    16201 cagcaaagaa accgatggtg cgatgacaga aacacatatg caatctctgg ataaaaaagc
    16261 gcggttacaa gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc
    16321 gaatgcgaaa gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca
    16381 gcaaaacgcc gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat
    16441 cggcatatta cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc
    16501 gctgtaaaac gctgactgct gcagcagcag tactattgat gttaaccgca ggctgttcca
    16561 ctctggagcg agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg
    16621 acgtatccaa aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac
    16681 cgctgatgtc cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag
    16741 gtcatgaagg tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga
    16801 ccaatattga taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac
    16861 gttaaaaaag gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc
    16921 tgctcgttgc ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg
    16981 atcgccatca tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt
    17041 tttagttaaa tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc
    17101 aaccgtcgcg ccctcctgca gcgtcactcg ctgcaggtat tgcttctcag gtagcgcata
    17161 agccacctca acggcaattt tacctagcac tgtaaacctc tttcgcacga accgtaaaag
    17221 cctggaccat attagccgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact
    17281 tattggtaaa ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg
    17341 taaacttcca tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac
    17401 tttggttact ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga
    17461 catctaccgc cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac
    17521 ctggcaaaaa ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc
    17581 tgtagggtac cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc
    17641 caacaaataa tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat
    17701 cgggttcatg ctaagataga gccttgtccc ccgcaggatt gatatggggt attttcgatt
    17761 tcagattacc gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca
    17821 gcgaccatcg cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa
    17881 gcgggacttg ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc
    17941 agcgacagct acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg
    18001 ccaatggccg tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt
    18061 ctcaaccagc gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta
    18121 gtggcgctct ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa
    18181 ggtaaaaaac agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa
    18241 gcacgcatca tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc
    18301 accgcgcctc cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca
    18361 aatgttgcca tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt
    18421 aacgataacg ccattgaggc tggtcatggc gctcataaat ctggtatact tacctttaca
    18481 cattggggct gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc
    18541 ggttggcctc gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca
    18601 gcttaataac ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag
    18661 agaggtcaaa cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact
    18721 taatcaggct agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact
    18781 gactaagcat gtagtaccga ggatgtagga atttcggacg cgggttcaac tcccgccagc
    18841 tccaccaaaa ttctccatcg gtgattacca gagtcatccg atgaagtcct aagagcccgc
    18901 acggcgcaag ccctgcgggc ttttttgtgc tccgaatttg tcccgcgaag tctgatgcca
    18961 actaattaaa tccgatcctt ttaggcacct tgttaggcac ttcataaagc tatattgtct
    19021 ttgaggtgcc taaaactatg gaaacccggc aatggcaaga caaaccaaac ctctatccgt
    19081 taaggaaatc gaatctgcta aacccaagga agcggactac gttctctatg atggcgatgg
    19141 ccttgagcta ctcattaaat ccagcgggag taaaatctgg cagtttcgct acattcgccc
    19201 tgtcaccaag acacgagcaa agaaaagcat aggcccttac ccgtcagtta cccttgccga
    19261 tgccagaaac taccgtgcag agtcccgctc actcctagct aaacagattg atccgcagga
    19321 acatcagcaa gaacaattgc gtagttcgct ggaagccaaa accaatactt tccagctcgt
    19381 agctgaacgt tggtggaatg tgaagaaagc cagtgtgaca gaggactatg cggaagatat
    19441 ctggcgctct cttgaaagag atgtctttcc tgcgattggc gacgttagcg ttacagatat
    19501 taaagctcat acactggttc aggccgtaca accggttcag gccagaggag cactggaaac
    19561 ggttcgtcgc ctgtgtcaac gtattaatga ggtcatgatc tatgcccaga acacagggct
    19621 gattgatgct gttcccagcg ttaatatcgg taaagccttc gaaaagcctc agaaaaagaa
    19681 catgcccagc attcgaccgg atcagctatc tcaactgatg cagacaatgc gaacagccag
    19741 cattagcctt tccacacgct gcctgttcat gtggcaactt cttactatta cccgccctgc
    19801 cgaagcggct gaagctcgct gggaagaagt agacatagaa gcgcaagagt ggaagattcc
    19861 cgcagcacgc atgaaaatga accgcgacca tactgttcca ttgtcagatg aagcaatagc
    19921 tgtgctggag atgatgaaac cgctaagcgg caaccgagaa tttatctttc ctagccgtat
    19981 caagcccaac cagcctatga acagtcagac tgttaacgcc tcactaaaac gcgcaggttt
    20041 tggtggtgtg cttgtttcgc atggtctgag atcaattgcc agtacggctc ttaatgaaca
    20101 aggctttccg cctgatgcca ttgaggcagc attggcccat gtggataaga atgaggttcg
    20161 tcgtgcttat aaccgcagcg attacctgga acagcgtcgc ccgatgatgc aatggtgggc
    20221 aaattttgtg atggcagcgg atcgtggaag catgattgaa ggtgggataa aaggaatgaa
    20281 gctagttggg tgactaaata gctgcgcggc atagtgtccc actttcagcc agcccttgca
    20341 ggttaagcca ccagacagta aacggcgggt gtcgcgttat taggcgcagc tatttagtag
    20401 taatattagg attggtgatt ctgca
//
LOCUS       DAJTYL010000072        20019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYL010000072 DAJTYL010000000
VERSION     DAJTYL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.072"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..149)
                     /locus_tag="OO572_002540"
     CDS             complement(<1..149)
                     /locus_tag="OO572_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR6661506.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVI"
     gene            complement(204..1055)
                     /gene="manZ"
                     /locus_tag="OO572_002541"
     CDS             complement(204..1055)
                     /gene="manZ"
                     /locus_tag="OO572_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6661507.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1068..1868)
                     /gene="manY"
                     /locus_tag="OO572_002542"
     CDS             complement(1068..1868)
                     /gene="manY"
                     /locus_tag="OO572_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6661508.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1931..2902)
                     /gene="manX"
                     /locus_tag="OO572_002543"
     CDS             complement(1931..2902)
                     /gene="manX"
                     /locus_tag="OO572_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6661509.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3365..4915
                     /gene="yoaE"
                     /locus_tag="OO572_002544"
     CDS             3365..4915
                     /gene="yoaE"
                     /locus_tag="OO572_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6661510.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4919..6517)
                     /locus_tag="OO572_002545"
     CDS             complement(4919..6517)
                     /locus_tag="OO572_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6661511.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6648..8012)
                     /gene="sdaA"
                     /locus_tag="OO572_002546"
     CDS             complement(6648..8012)
                     /gene="sdaA"
                     /locus_tag="OO572_002546"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6661512.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8196..8774)
                     /locus_tag="OO572_002547"
     CDS             complement(8196..8774)
                     /locus_tag="OO572_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6661513.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8778..10139)
                     /gene="pabB"
                     /locus_tag="OO572_002548"
     CDS             complement(8778..10139)
                     /gene="pabB"
                     /locus_tag="OO572_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6661514.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10213..10392
                     /locus_tag="OO572_002549"
     CDS             10213..10392
                     /locus_tag="OO572_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6661515.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10512..10871)
                     /locus_tag="OO572_002550"
     CDS             complement(10512..10871)
                     /locus_tag="OO572_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6661516.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11233..11577)
                     /locus_tag="OO572_002551"
     CDS             complement(11233..11577)
                     /locus_tag="OO572_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6661517.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11709..13619
                     /locus_tag="OO572_002552"
     CDS             11709..13619
                     /locus_tag="OO572_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6661518.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13677..14372
                     /gene="tsaB"
                     /locus_tag="OO572_002553"
     CDS             13677..14372
                     /gene="tsaB"
                     /locus_tag="OO572_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6661519.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14412..14993
                     /gene="yeaY"
                     /locus_tag="OO572_002554"
     CDS             14412..14993
                     /gene="yeaY"
                     /locus_tag="OO572_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6661520.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15199..16884
                     /gene="fadD"
                     /locus_tag="OO572_002555"
     CDS             15199..16884
                     /gene="fadD"
                     /locus_tag="OO572_002555"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6661521.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16966..18081
                     /gene="rnd"
                     /locus_tag="OO572_002556"
     CDS             16966..18081
                     /gene="rnd"
                     /locus_tag="OO572_002556"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6661522.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18135..19100)
                     /gene="yeaX"
                     /locus_tag="OO572_002557"
     CDS             complement(18135..19100)
                     /gene="yeaX"
                     /locus_tag="OO572_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6661523.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19156..19773)
                     /gene="yeaW"
                     /locus_tag="OO572_002558"
                     /pseudo
     CDS             complement(19156..19773)
                     /gene="yeaW"
                     /locus_tag="OO572_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         4917 a         5014 c         5214 g         4874 t
ORIGIN      
        1 aagataacgc tatcgatgcg accacgccgg agcaaaggaa ttgccagcag ggtggggccg
       61 ttacggcggg gcatgatgaa ctgatcgtag atcgcgaagg ccaggagtgc ggcgatgaaa
      121 agaatcagta ccaggtccgt gattgtcatt aatcctccag ataaaaaaac ggggccaaaa
      181 ggccccggta gtgtacgaca gtcttacagt cccagcaggc cgcaagcgta accagcgata
      241 ccgatgacga agaagccaac gatgatccac agcgggttaa cttttttgcg cagcagccac
      301 atacaagcaa atgtcagcag cagtggtaca aggcctggca ttaactggtc aagaatagtt
      361 tgcacggtgg tgacacgggt ctgtccatcc tgcccggtga tggtcgaaac caccagcggg
      421 atgttgacat gtgtccactt gttaaccaat gcccccatga caaacaggcc gaggatagac
      481 gccccttccg tcagtttttg caggaagcca ccacccatat ctttaacgat atcgatacct
      541 ttggagtaac catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacagg
      601 atgaagaaca gcagcggacc taacaggctg ccgctcatcg cgataccggc acccagtgct
      661 gcaaataccg gacgtacggt tccccagaag atcgggtcgc ctacaccagc cagcggcccc
      721 atcaaaccga ctttgatacc gttgatagca ccgtcatcga tctctgcgcc attagcacgc
      781 tgttcttcca gcgccagggt tacgccgaga atcggtgcag ccacgaacgg ctgggtgtta
      841 aagaactcca ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg
      901 cgacgaattg ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc
      961 catgaaccct ggaagaggtt agaacgcagg aatacgccac gaatatcact ttgagtgagt
     1021 tttttctcgg tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg
     1081 ttatcgagat cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc
     1141 gggctaagtt ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg
     1201 ttaaagttgg tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca
     1261 gcacgcatca tgttgataac catcgcataa ccaaccacca cgatcatgcc cccggcgata
     1321 ttcagaccat tggtcaccac ttccggaatc gcattcagca tgttctgtac ttcgctggta
     1381 ccaacagaca gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga
     1441 gaagaaacgt ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca
     1501 gcgtgctgga aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg
     1561 gccagaggga ttgccagtgc gatacctgca ccaatgctct gatgacctgc gataaccaga
     1621 atggtagaaa tgatagaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc
     1681 cagcccagcg cgatcatttc cagcgtacca ccgataataa taccggtttt catatcccca
     1741 agaacgatac ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg
     1801 attgatccca tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta
     1861 atctccattg tacttctcct gtttacgtaa gacttaagtg tgaaaactga atgataatca
     1921 acacaatacg ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg
     1981 tggaaacctt acggacttcc agctcaatac cgcgtgcatt cagttttttg aacgcctcga
     2041 tatctttttc atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca
     2101 taccaccgac gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg
     2161 ttgggttggt aaataacagc attacgcgtt cgccagcata tttcgggttg ttgtagacgc
     2221 gaatcatttt ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga
     2281 gcagtgtctt acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga
     2341 cattggtttc tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac
     2401 gcgcaaggcc aataaccatg tagtcgtttg gccccattgg ttttgccgga gttggagccg
     2461 cttttggtgc tgcggcagcg ggtgctggcg cggctttttc aaccggtttg gctttcagtg
     2521 ctttcacgcc ttcacggcct gtttctactg ccagcgcaac cagttcatca aagctcgggt
     2581 catcatcacg ggccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt
     2641 cataatgctc tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg
     2701 tatcaacgag aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtatt
     2761 tttcaatcag cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct
     2821 gctcgcctaa cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc
     2881 ctataacaat agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc
     2941 cagatgtatt tttgaatcgt cgtccacaat cgaatcgatt cagataaggg ctacaaccaa
     3001 aaacccctgc gtttcgcgat ttattttaga tatcgaaaaa attattttat gtgatgaaga
     3061 tccgtaattt aactttcgat tatcagaaat ttcgaaaggt aaaatatcct tgtcacattc
     3121 gtttgcaaag gaaggtaaat ctttgccaaa tcagaggcgt ctctgatatg tttaactccc
     3181 gtttaacaac catggagtat agggcagtag cccgcagtat ggatcgtcac cgacgtcatt
     3241 tcagcatcag gccttttaac gcctgccttt ctggcactct atgccgcacc tttcgtttgc
     3301 attttgtcgt tacgcctgca ttatttctgg cgtcgaatag ctattcctta agcaggagct
     3361 tgtcatggaa ttcttaatgg acccctcaat ttgggcgggg ctactcacgc ttgttgttct
     3421 cgaaattgtg ctgggtatcg ataacctggt cttcatcgcc attcttgctg acaaactgcc
     3481 gccaaaacaa cgcgataaag cgcgtttgct ggggttatca ctggcgctga ttatgcgtct
     3541 ggggctgctg tcgctgattt catggatggt cacgctgacc aaaccgctat ttaccgtcat
     3601 ggatttctcc ttctccggac gcgatctgat tatgttgttc ggggggatat tcttgctgtt
     3661 caaagcaaca accgaactgc atgaacggct ggaaaaccgc gatcatgatt ccggccacgg
     3721 taaaggctac gccagtttct gggtggtcgt cacacagatt gtcatccttg acgccgtctt
     3781 ctcgttggat gcggtaatta ctgcagtagg gatggttaac catctgccgg tgatgatggc
     3841 ggcggtagtg attgcgatgg cggttatgtt gctggcatcg aaaccgctga cgcgattcgt
     3901 taaccagcac cccacggtgg tggtgctctg tctgagcttc ctgttaatga ttggtctgag
     3961 tctggtggca gaaggtttcg gtttccacat tccgaaaggt tacctgtatg ccgcgattgg
     4021 cttctcgatc attatcgaag tgtttaacca gattgcgcgt cgcaacttta ttcgtcacca
     4081 gtcgactttg ccgctgcgag cgcgtactgc cgatgccatc ctgcgtttga tgggcgggaa
     4141 acgtcaggcc aatgttcagc acgatgccga taacccgatg ccgatcccgg aaggtgcatt
     4201 tgccgaagaa gaacggtaca tgattaacgg cgtactgacg ctggcgtcgc gttctctgcg
     4261 cgggatcatg acgccgcgcg gtgaaataag ctgggttgac gctaatctcg gggtcgatga
     4321 aatccgcgag caactgctct cttcaccgca cagtctgttc ccggtatgtc gcggtgaact
     4381 ggatgaaatc atcggtatcg tacgtgctaa agaactgctg gtggcgctgg aagagggcgt
     4441 tgatgtggcg gcgattgctt cggcgtctcc ggcgattatc gtcccggaaa ccctcgatcc
     4501 gatcaacctg ctgggcgtgc tgcgtcgcgc tcgcgggagc tttgttatcg tgaccaacga
     4561 gtttggtgtg gtacaaggct tggtcacgcc gctggatgtg ctggaagcca ttgcgggtga
     4621 attcccggac gctgacgaaa cgccggaaat cattaccgat ggtaacggct ggctggtaaa
     4681 aggcggtaca gatttgcatg ccttgcagca ggcgcttgat gttgagcacc ttgccgatga
     4741 cgatgatatc gcgacggtcg cgggcctcgt gatctcggca aatggtcaca ttccccgtgt
     4801 gggcgatgtg attgatgtag ggccactgca tatcaccatc tttgaagcca atgattatcg
     4861 tgttgatctg gttcgcattg ttaaagagca accggcgcac gatgaagatg agtaagcgtt
     4921 aacgtaacga cataatgggc gtgatatgtc cattatgccg ggcgggcggc ggttggctgc
     4981 ccgccagcca tttgggaaaa tcacgtagcg gcatcggctg tgcgtacaaa tacccttgca
     5041 aaatatgcac gccatgacgg cgcaaatact tcgcctgttc ttgcgtttcc acgccctccg
     5101 ccaccagttc aatattcagt ctttgcccca gcgcgatgat gatatcggtc accgtcgagt
     5161 taaccgcgtc agaacctata gctgcggtaa atgacttatc aattttcagc acgtcaggac
     5221 gtaatgtttc aagccaggaa aacgagctgt tgccggtgcc gaagtcatca atcgccagtt
     5281 tgacgttttt acgatgcagc tcgcgggcaa tccgataatc aacatccagt aaggcatcgc
     5341 gttcggtgat ttcgaggatc agttgctgaa tcgggtgagc gctaaaccag tactgattga
     5401 gatcttttat cagcacacca cggcgaaaat ggctgggtgc gacgttaatg ccaacatgaa
     5461 actgactact catcgggaaa acatggtgct gacgaatggt ttctgccatc acatagtggg
     5521 tcagtggcac aattaaatga tgttcttccg cgataggaat aaacacatcc ggtgaaatcc
     5581 agccctgacg cggattattc cagcgcagca aaatctctac accaatacat tgctggctgc
     5641 gcgcattaag caaaggctga cagaacaatt cgaactcatg ttgcgccagg cccagattaa
     5701 tttcgcggga aaagctcatc cggtaagcgg tcgccagcca ggcgatatag cccaccagta
     5761 aacttagcag caccgccagc ggtaattgtg ttggaagata gtgccatgcc agcgccgtag
     5821 cccccggacc atttacgtta atggtaaaag gaaagctttg cgaagaaacc tgatagtttt
     5881 cattgtcttc aggttgcgga agggaatcta ccagcccatt accatagagc aaatgccgtt
     5941 tgtccaccgt caggctggcg ctactgattt gcggcagttg tggctcaagg agcattgccg
     6001 tcagtaagtc gatgttaatc atctctatga ccccagcatt gctactgccc gctgcaggcg
     6061 tccattgaat caaaaccgga ctgcctttaa tcagggcacg gtcgatcgtc aggcgtaaaa
     6121 gtggttgcgg tgcaggaagt tcagccagaa tgtccacgac gggcacattg cgataaccaa
     6181 aaatgctgga gcaataaagt gtgccgtctt gcaccaggcc aatggatcgc acagtttgga
     6241 gttttgccgc ctgtttacgt aatggcaaat gggcgacaga gcagggcaga ccaatcagcg
     6301 gaagcaagac tttgctacct gcctgtaggg gaagcagtac tttatctaat tcctctacag
     6361 cgtgattggc gaattgtact acccgttgtt gatttaagtt acgctgtgaa ataaatcgca
     6421 cgctcagggt cgaagcgagc gtaacgagtg cgcaaatcgt acaaacaatc attcgattac
     6481 ggcgataggt tttaatgatc cgttgtgctt tttgcatgcg ggccaccctg taagcgtatg
     6541 gcgacaaacg cctgacgcaa cagtggaagt gtagtgggga atattacagc agacgagaaa
     6601 gcgggtataa attcgcccat ccgttgcaga tgggcgagta agaagtatta gtcacactgg
     6661 actttgattg ccagaccacc gcgtgaggtt tcgcggtatt tggcgttcat gtccttaccg
     6721 gtttcgtaca tcgtttcgat gaccttatcc agcgagacgc gcggtgcact ggtgcggcgc
     6781 agagccatcc gtgcggcgtt aatcgccttc acagaggcaa tggcattacg ctcaatgcac
     6841 ggcacctgaa cctgccctgc aaccgggtcg caggttaaac caaggttgtg ttccatgcca
     6901 atttccgccg ccacgcaaac ctgttccggg ctaccgccca gcagttctgc aagacccgca
     6961 gcagccattg aacaggcaac acccacttcg ccctggcaac caacttccgc accggaaata
     7021 gaggcgttca ttttatacaa tgcaccaatc gcgcccgctg ccataaagta acgggtatag
     7081 atgtctgggc tgaccgattc aataaagtgg tcatagtaag ccagcactgc cggaacgata
     7141 ccgcaggcac cgttggttgg cgcagttacc acacgaccac cggcggcgtt ttcttcgtta
     7201 actgccagcg caaacatgtt tacccagtca atgacattca tcggatcgtt agacagttta
     7261 tcgctggaaa ctaacatccg gcgcagggca gacgcacgac gtggcacgcg cagcgggcct
     7321 ggcagtacac cttcggtgtt catcccgcga tcgatacatg cctgcatggt ttgccagaca
     7381 tgcgcgaaat actcgtcgat ctctttcttg ctgtgcagcg ccagttcgtt ctgcatagcg
     7441 agaccagaca gcgaatagcc ggtttcatta cagtacgcga gcagttcggt ggcagatttg
     7501 aacggatacg gcacgctcac ttcgttggca gcatcctgac caaagtgttc ttcatcgacg
     7561 ataaaaccgc cgccgatgga ataataagtt ttgctgtaga cgacttcatc gccgttatag
     7621 gcgtggattt gcataccgtt ttcatgcagc ggcaggttgc cgttatgaaa acgcatcccg
     7681 ttgtcgcgcg ggaaatccac ttcatgccgt ccctgtgcca gcagcagacg ttcgcgctct
     7741 tttacgtcgc gaataaaacc gggaatactg tcgatatcca cggtggcagg ttcgttacct
     7801 gcaagaccca taataatggc gatatcggtg tggtggcctt tacccgtcag cgacagtgaa
     7861 ccataaacgt ccacggcaac gcgagtaacg ctatccagta agcctttttc gaccagatca
     7921 tcgacgaact gtttacccgc cttcataggc cctacggtat gggaagatga gggaccaatc
     7981 cccaccttaa acatgtcgaa tagactaatc acgataatac tcctgacaag gtgactggac
     8041 ttccagtaac gaactaataa cagcgcatag tgtaagaggg aacgcggcga ctggcttaac
     8101 tattcacatg aattaaacta atcaaaaacc gcgatttaac taatattgcg atgattgtct
     8161 caagtagtgt tttgtagatg taaagataag tatagtcagg gggtcacacc aatttgcagc
     8221 gccagctcac gaattatgcc tgcggtcatt ccccatacaa aatactgttc gtaccaggac
     8281 agccataccc gatgtgaatc gccacggcgg tagatatcta aagggtgata acgacccaga
     8341 tgtaatgcct gggcgagcgg catttcaaac accgccgaga cttcatcttc actggcgcga
     8401 tacggcagat cgggcgggat aatgccgacc actggggtta cttgatagcc agttacgcta
     8461 tcgacgggcg gcagcacgcc gataacttca acggcggaag gcggtatagc gacctcttct
     8521 tcagcttcgc gcagcgcggc ggcgataact gatgcgtccg tgtcatcgac tgcacctcca
     8581 gggaatgcca cttgtccagc gtgtttacgc agatgaattg aacgctgagt cagcaacaac
     8641 cccggttgcg gtcgacggac gatggggatt aacacggcag cctgacgatg atttaggggt
     8701 tcccggttaa tttgcgggcg caaaagttga aagcgagata aaaaatcatc aagcgtcagg
     8761 ctacggtatt ccacgtctta cttctccagt tggcgtaata tcttattaac tttatcaaaa
     8821 gtttcctgat attccgcttc ttcctggcta tcggcgacaa ttccaccgcc cgcagagcag
     8881 tatatttgtc cgttaatggc agtcagcgtg cggatagtaa tgctggtatc catgttgccg
     8941 caaaagctca aatagccaat gctgccgcac caggcattac gtcgctgcgg ttccagttcg
     9001 tcgataattt ccatagcccg tactttcgga gccccggtta ttgagccacc aggaaaagct
     9061 gcgcgcagca gatcgctggc gtgtaactgt tctggtagtc gcgccgttat agtgctgacc
     9121 agatgatgca cggcagggaa gggttccacc acgaagagct ctggtacttt taccgaaccg
     9181 gctacggcaa cacgaccgat atcattacgc attaaatcga caatcatcag attttcggca
     9241 cgatctttcg ctgagttcgc cagtttttct gcttgtttgc tatcttcctg aggatcgggc
     9301 aggcgtggta gcgtgccttt aatcgggcga gtctggattt cactattatc acaaagaata
     9361 aaccgctctg gcgaaaggct taaaattgca ccctgttcaa gacgtaaaaa agcgctaaat
     9421 ggcgcgcggt tggcctgatt aagctgaagg aatgcctgcc attcatcgcc agaataggtc
     9481 gcatgaaaac gctgggcgag attcacctga tagcaatcac cgctgtgcag atattcctgt
     9541 acctggcgaa atttttcgcc gtactgctcg tgggtcatat tggattgcca gtcggaagtg
     9601 agcgtgaaat cttcctgcgg cgagaattgc tggctttcca gccaggcccg acgggcattg
     9661 acatcattat gactcagcaa agaaactgta tgacgctggt ggtcgacaat gagcgcccaa
     9721 tcgtagatac ccactgccat atccggcaga acgatatctt gttccgcaat ttctggcagt
     9781 gactcaaaac ggcggcccag atcgtagcca aacaacccca gtgcgccgcc ctgaaatggc
     9841 aaatcttcgt tatgcgttgg gcgaatgtct gcgcgatcca gcacctgctg gagcacctgt
     9901 agcgggtcat cagtggtcgt tgtgcgtttt tcgctttcac taacaacggt ttctttacct
     9961 aaagtggtta aagtgcaaat cggatcggcg accacaatat caaagcggct atacggatga
    10021 tcggcatagc cggagtgtaa aagcatcgcc cacggcaggt ggcttaagcg ggagaaataa
    10081 aattcagcgg cgtcctgacg ccagggtaaa gtaatcacag tgggagataa cgtcttcatc
    10141 agtcctgact ctactggcta tgtgccgggt agcatgaaat aattagcgcc aacaatttag
    10201 caggagttaa caatgtttgc aggtttacct tcactcatcc atgaacagca gcaaaaagct
    10261 gtcgagcgga tccaggaact gatggcacaa gggatgagta gcggccaggc aatcgcgctg
    10321 gtggcagaag agctgcgtgc caaccactcc ggcgagctaa tcgtggcgcg ttttgaggat
    10381 gaagacgagt aacacactaa tccgtatcga tttcgttata gaagccgatg atcatttgat
    10441 tgtgagaaaa aggacgcttt ttatatgtga ttaaaagcgt cctgacccat ttatgctgat
    10501 ataaatttaa ctcagaccag tgcttttagt tcttcctgca tatatgttga aaagtattca
    10561 ggatttttaa tcacagagcg ttcacctgtc tccatttttt ttgcccatcc aaccattttt
    10621 tccgtgaacc cagggttcca gccttcctgg tcagctcgcg ccgtaatatc agcggcactc
    10681 gcgggtactc ccagttcttt taaatattta aagattccct tcgccgtgct ttcattcatc
    10741 gaacttggtg ttggcgctga tacatccatg gcaccaatga aatccagggc tttatctatt
    10801 acagcaggca tattcttatc cttaaaatta accatgttat cgataaggct atatatgacc
    10861 ttaatttgca tctaatatca aagggattat aacgatatgg cacgagttaa cctgttgaca
    10921 ttcaggtgtt ttaaaggtag cagctcatgg tgtgttatcc agtaagatgg agaaaattaa
    10981 cggtcagccc gcacgctata accagattgt tcagctaaga aagaaaacca gagttgctaa
    11041 tcaaagggcg aacacaacgg catggttatg tttatttata tagttagttt tatagataaa
    11101 attgaaatta taaatataaa taatgaatta gcagaagcat caacagtaat atcttattct
    11161 ctggtcgatt tatgtaaaac cgaccagtat tatcgataaa ttatcgtctg acttattttg
    11221 cttcgataaa gcttacaccg cagccacaat cttaatttca actttatact tcgggttcat
    11281 caaacccgct tgtaccgtgc agcgcaccgg cgcatgaccc gcgacaaccc aggcatccca
    11341 cgctttattc atcgccgcga agtcgttttt atcggccagg aaaatggtgg catccagaat
    11401 gctcgattta ttgctgccct gtttttccag cacggcgtca atctgtgcca gcgtgttggc
    11461 ggtttgctca aaggcatcgg cgtcgaggtt ttccggtaca ccagtgtagt agagcgtgtt
    11521 gttgtggatt actacatcag accagcgggc ttcagcatcg atacgaacga tagtcatcat
    11581 tttgtcctca ttatacttcc attttcgtgc tgcaaagact gccacaatgt gaggttgccg
    11641 tcaccacttc aactggcgaa agcgccctca tcctgacata atccctgttc aaatcaacag
    11701 ggggtagtgt gacggacgat tttgcaccag acggtcagct ggcgaaagcg ataccaggct
    11761 ttaagccgcg agaaccacag cgacagatgg cggtagccgt cacccaggcg atagaaaaag
    11821 gccagccgct ggtggtggaa gcaggaaccg gtacgggcaa aacctacgct tacctggctc
    11881 ctgcgctgcg ggcgaaaaag aaagtcatta tctcgaccgg ctcaaaagcg ttgcaggatc
    11941 agctctacag ccgcgatttg ccaacagtct caaaggcatt gaaatatacg ggcaacgtgg
    12001 cgctgctgaa agggcgctca aactacctct gcctcgaacg tctcgaacag caggcgctgg
    12061 cggggggcga tctgccggta caaatcttaa gcgatgtgat cctgctgcgc tcctggtcta
    12121 atcaaacagt cgatggtgat atcagcacct gcgtcagcgt ggcggaagat tcacaggcgt
    12181 ggccgctggt caccagcacc aacgacaact gtcttggcag cgactgcccg atgtataaag
    12241 attgctttgt ggtcaaagca cgtaaaaaag cgatggacgc cgatgtggtg gtggtaaacc
    12301 atcatctctt tctggcggat atggtggtta aagagagtgg atttggcgag ttgatccctg
    12361 aagcggacgt catgatcttc gacgaagccc accagctacc ggacattgcc agccagtatt
    12421 ttggtcagtc actctccagt cgacaactgc tcgacctggc aaaagacatc accatcgcct
    12481 accgcaccga attaaaagac acccagcagt tacaaaagtg cgctgatcgt cttgcccaga
    12541 gtgcgcagga ttttcgtctg caactcggtg agccaggtta tcgcggtaac ctgcgtgagc
    12601 tgttagctaa tccgcaaatt cagcgggcat ttttactgct cgatgacacc ttggaacttt
    12661 gttatgatgt ggcgaaactg tcactggggc gttccgcctt gctggatgcg gcatttgagc
    12721 gcgccacgtt gtatcgcaca cggctgaagc ggctaaaaga gatcaatcag ccgggctaca
    12781 gctactggta cgaatgcact tcgcgccatt ttactctggc tctcacgccg ctcagcgtgg
    12841 cggataaatt caaagagtta atggcgcaaa agcccggtag ctggatcttc acctcagcaa
    12901 cgctgtcggt gaacgacgat ctgcatcatt tcacctcgcg gcttgggata gaacaggcgg
    12961 agtcgttgct attgccaagc ccgtttgatt acagccgcca ggcgttactc tgtgtgccgc
    13021 gcaatctgcc gcaaaccaac cagccaggtt ctgctcgcca gttagcggca atgctgcgac
    13081 cgatcatcga agctaacaac ggtcgttgtt ttatgctttg tacctcgcac gccatgatgc
    13141 gcgatctggc cgagcagttc cgcgctacca tgacgcttcc cgttttgttg cagggggaaa
    13201 ccagtaaagg ccaactgttg cagcagtttg tcagtgccgg taatgcgctt cttgtggcaa
    13261 ccagtagttt ctgggaaggg gtggacgtgc gtggcgatac attgtcattg gtaattatcg
    13321 acaaattgcc gtttacctcg ccggatgatc cactgttaaa agcgcgcatg gaagattgtc
    13381 gtttgcgcgg tggcgacccg ttcgatgaag tgcaactacc agatgccgtc attactctca
    13441 aacagggggt aggtcgactg attcgcgacg ccgacgatcg tggcgtgctg gtgatttgtg
    13501 actatcggct ggtgatgcgt ccttacggcg cgacgtttct cgccagtctg ccgcccgcgc
    13561 cacgcacccg tgacattgcc cgtgcggttc gtttccttgc gataccatcc tccaggtaat
    13621 ttgttacgga gtatggtatg atgcgcgcct ttttattgat ctaagcacga agacccatgc
    13681 gaattctggc tatcgatacc gcgacagagg cctgctctgt cgccctgtgg aacgacggta
    13741 ctgtcaacgc tcattttgag ctttgccctc gtgaacatac tcaacgaatc ttaccgatgg
    13801 tgcaggatat cctgaccacc agcggaactt ccctgactga tattaacgct ctggcttacg
    13861 ggcgcggccc cggtagcttt accggcgtgc gcattggtat tggcatcgcg caagggctgg
    13921 cgcttggcgc ggaattaccg atgattggcg tctccacgct aatgacgatg gcgcaagggg
    13981 cgtggcgcaa aaacggcgca acacgcgtgc tgtcagccat tgatgcgcga atgggcgaag
    14041 tttactgggc cgaatatcag cgtgatgaaa acggtatctg gcacggtgaa gaaaccgaag
    14101 ccgtactcaa acccgaactc gttcatgaac gaatgcaaca gctttccggc gaatgggtga
    14161 cggtagggac gggctggcaa gcctggccgg atctcggtaa agagagcggg ctggttttgc
    14221 gcgatggcga agtgttactc cccgctgcag aagatatgct gccgattgct tgtcagatgt
    14281 ttgccgaggg taaaacggtg gcggtggaac atgccgagcc ggtttatttg cgtaacaacg
    14341 tcgcatggaa gaaacttccg ggcaaagaat gaatcttaag agtatgccct gagaaaaaaa
    14401 ggagtcgcat catggcggtt caaaagaatg ttatcaaagg catactggca ggtacgtttg
    14461 cgctaatgct gagcggttgt gtcactgtgc cggacgccat taaaggcagc agtaccacgc
    14521 cgcaacaaga tttagttcgg gtgatgagtg cgccgcagct gtacgttggt caggaggcac
    14581 gctttggtgg caaagtggtt gcggtacaaa accagcaagg gaaaacccgc ctggaaattg
    14641 ctaccgtacc gctggacagc ggagctaggc cgacgctggg agaaccttct cgcggtcgca
    14701 tttatgccga tgtgaacggt tttctggacc cggtggattt ccgtggacaa ctggttacgg
    14761 tagtcgggcc aatcaccggt gcggttgacg gcaaaatcgg caatacgccc tataaattta
    14821 tggtgatgca agtaacgggt tacaaacgtt ggcatttaac ccagcaggtg attatgccgc
    14881 ctcagccgat tgatccatgg ttttatggcg gtcgtggctg gccctatggc tacggcggat
    14941 ggggctggta taatcccggc cccgcgagag tacaaacagt tgtaaccgaa taattgcttg
    15001 tttttaaaga atgcagaaac agcggctggt ccgctgtttc tgcattctta cggtaaagat
    15061 aaaaataaat agtgacgcgc ttcgcaacct tttcgttggg taattatcaa gctggtatga
    15121 tgagttaata ttatgttaac ggcgtgtata tcatttgggg ttgcgatgac gacgaacacg
    15181 cattttagag gtgaagaatt gaagaaggtt tggcttaacc gttatcccgc ggacgttccg
    15241 acggagatca accctgaccg ttatcaatct ctggtagata tgtttgagca gtcggtcgcg
    15301 cgctacgccg atcagcctgc gtttgtgaat atgggggagg tgatgacctt ccgcaagctg
    15361 gaagaacgca gtcgcgcgtt tgccgcttat ttgcaacaag ggttggggct gaagaaaggc
    15421 gatcgcgttg cgttgatgat gcctaactta ttgcaatatc cggtggcgct atttggcatt
    15481 ttgcgtgccg ggataatcgt cgtaaacgtt aacccgttgt ataccccgcg tgagcttgag
    15541 catcagctta acgatagcgg cgcatcggcg attgttatcg tgtctaactt tgcccacact
    15601 ctggaaaaag tggttgataa aaccgccgtt cagcacgtaa ttctgacccg tatgggcgat
    15661 cagctatcta cggcaaaagg cacggtagtc aatttcgttg ttaaatacat caagcgcttg
    15721 gtgccgaaat accatctgcc agatgccatt tcatttcgta gcgcactgca taacggctac
    15781 cggatgcagt acgtcaaacc cgaactggtg ccggaagatt tagccttcct gcaatacacc
    15841 ggcggcacca caggtgtggc gaaaggcgcg atgctgactc accgcaatat gctggcgaac
    15901 ctggaacagg tcaacgccac ctatgggccg ttgctgcatc caggtaaaga actggtagta
    15961 acggcactgc cgctgtatca catttttgcc ctgaccatta actgcctgct gtttatcgaa
    16021 ctgggtgggc agaacctgct tatcactaac ccgcgtgata ttccagggtt ggtaaaagag
    16081 ttagcgaaat atccgtttac cgctatcact ggcgttaaca ccttgttcaa tgcgttgctg
    16141 aacaataaag agttccagca gctggatttc tccagtctgc atctttccgc aggcggtggg
    16201 atgcctgttc agcaagtggt ggcagaacgc tgggtgaaac tgaccggaca gtatctgctg
    16261 gaaggttatg gcctgaccga gtgcgcgccg ctggtgagcg ttaacccgta tgatattgat
    16321 tatcatagcg gtagcatcgg tttgccggtg ccatcgacgg aagccaaact ggtggatgac
    16381 gacgacaacg aagtaccacc gggtcaaccg ggtgagcttt gtgtcaaagg accgcaggtg
    16441 atgctgggtt actggcagcg tccggatgct acagatgaga tcatcaaaaa tggctggtta
    16501 cacaccggcg acatcgcggt gatggatgaa gaaggattcc tgcgcattgt cgatcgtaaa
    16561 aaagacatga ttttggtttc cggttttaac gtttatccca acgagattga agatgtcgtc
    16621 atgcagcatc ctggcgtaca ggaagtcgcg gctgttggcg taccttccgg ctccagtggt
    16681 gaagcggtga aaatcttcgt agtgaaaaaa gatccatcgc ttaccgaaga gtcactggtg
    16741 actttttgcc gccgtcagct cacgggatac aaagtaccga agctggtgga gtttcgtgat
    16801 gagttaccga aatctaacgt cggaaaaatt ttgcgacgag aattacgtga cgaagcgcgc
    16861 ggcaaagtgg acaataaagc ctgagcgtta agtcagtcgt cagacgccgg ttaatccggc
    16921 gttttttttg acgcccacta aagagaaaac aatttgaatt accaaatgat taccacggac
    16981 gatgcgctgg cttctttgtg tgaagccgtc cgtgtctttc cggcgatagc cctggatact
    17041 gaatttgttc gtacgcgcac ttattacccg cagctggggt tgattcaact tttcgatggc
    17101 gagcatctgg cgctaattga tcctctcggg atcaccgact ggtcaccgct gaaagcgatc
    17161 ctgcgcgatc cgtccatcac aaaatttctc catgcaggca gtgaagatct ggaagtgttc
    17221 ctcaatgtct ttggcgaatt accacaaccc ttgattgaca cgcaaatcct tgctgccttc
    17281 tgcggacgcc cgatgtcatg gggtttcgct tccatggtgg aagagtattc cggcgttacg
    17341 ctggacaaga gtgaatcgcg caccgactgg ctggccagac cgctgaccga acgtcagtgt
    17401 gaatacgcag cggcggatgt ctggtatctg ttaccgatca ccgccaagct tatggtagaa
    17461 acggaggcct ccggctggct acctgcggcg ctggatgaat gccgcctgat gcaaatgcgt
    17521 cgtcaggaag tcgttgcgcc ggaagatgcc tggcgtgata tcaccaatgc ctggcagtta
    17581 cgcacacgcc aactggcctg tctgcaactg ttagccgact ggcgactgcg caaggcgcga
    17641 gagcgtgatc tggcggtgaa ctttgtcgtg cgtgaagagc atttgtggtc ggtcgcgcgt
    17701 tatatgccgg gaagtttagg cgaactggac agcttgggtt tatccggtag cgaaatccgc
    17761 tttcacggta aaacgctgct ggcgctggtg gaaaaagcgc agacattgcc ggaagatgcc
    17821 ttaccgcagc cgatgcttaa cctgatggac atgcctggtt atcgtaaagc gtttaaagcg
    17881 attaagtcgc tgattactga cgtgagcgaa acgcataaga tcagcgccga attgctggca
    17941 tcgcgtcggc aaatcaacca actgctgaac tggcactgga aactgaaacc gcagaacaat
    18001 ttgccggagc tgatttccgg ctggcgtggt gagctgatgg cggaagcatt acacaattta
    18061 ttgcaggaat atccgcagta aaatcttccg aagccggact gggcgcgctc agcgccacat
    18121 ccggctttgg caaactacaa atccaacacc aggcgtttac ccttcgcacg cgaacaacag
    18181 atcaacatac tttgctggct ggcacgctct tcatcgctaa aatactgatc ccgatggtca
    18241 gcttcacctt ccagtattgc cgtttcgcag gttccgcata ccccttcacg acataaacat
    18301 tccactttcg cggctttatt attttcaata acctgcaaaa tagtcatctc ttccggcacc
    18361 acaaactctt ttccggaacg ggcaagcacc agggtaaatg catcgccggt tttgtcttcg
    18421 atagcaaatt gctcaaagtg cagtgtatcg gcggcgatgt ccagacgcgc agcttcactt
    18481 cttaccgctt caattagcgc ctcggggcca caggtataaa cgtgtgtgcc aggttcgata
    18541 tccgccaata atcgcgctaa ttccagtcgt gttccggttg atgaatgatg caaatggact
    18601 ttctcagcct gcggatgctg gactagctca gcacgaaatg cgcaactttc tggatttcgc
    18661 gagcagtaat gtagctgcca gtcgatgtcg ctgtgttgca actctgccat gtgcgacagg
    18721 aaaggggtga taccaatacc gcccacgata aacagatgct ttctggctga gggaattagc
    18781 gcaaacaggt tattaggcgt tgagatcgtt aaccgatcgc ccacttttac ctgctgatgc
    18841 aaaaagcggg aaccgccgcg cgagttttct tccagtcgaa cggcaatctg ataactagag
    18901 gtgttatgcg gtgaactcag tagtgaatac gcattgctgt actggttatc accatcgctc
    18961 atctgcacta ggatgtgact tcctccggtg aacgcaggca gcggtttgcc atcggttgcc
    19021 accagcgtga agcgtttcac ctgttcggta aggggttcaa cctggctcac ctgtacttca
    19081 aacatttgat agtctgacat tgctgtactc catcaaaaat cagcccagcg gcggaaaata
    19141 ccgccgccga tgtcattagt ccttaaacac ctgcgccagc agattatgga aatgggcgat
    19201 accatgttcg gaaatgccac taccgctact gtcggccatg atgcgcccct gaccacgata
    19261 gccacgcgat ttcagccctt tctgtacgct ttcaaccaga cgtaaatctt ccggatggaa
    19321 cacatcgcga taccactcaa tcagcgattt ttgctcgtcg gttaactctt cattggtgaa
    19381 gtaaatatcg tagttttgca gggtagtttc agaatccacc gggaattcat aaatgaccgt
    19441 catcatccct ttgatcgggg tgacgttcag catcgtgcac ggccacagcc agaaaccgtg
    19501 gaatgccgca tccgtaccct cttcaaattt aaacgactgt tcggacggtt tggcgaaacc
    19561 gtattgcagc gtccagttac cgtgcatggt gtgccagtaa cgatcaacct gtacggagtc
    19621 ggagaaacct agatgcgccg gaccacagtg atagcactcg agatagttat cgacaatgtt
    19681 cttccagttg gcaggcgtgc gggtggtaaa gcgggccgct agtttcagat tgtggacttc
    19741 cgggcaggct tccagcactt tcgcgcccag accggtgtat tgattcactt gaagtacgaa
    19801 aaaaaaccgg gaggacattg gattattcgg gttctgatgg gattagattt ggtggggctt
    19861 gcaagcctgt agcgcaaatt ttagtcgtta atcaatgaaa cgcgaaagat agtaaaaaat
    19921 tgcttttgtt tcattgaaaa tacgaaaaac aaaaacactg caaatcattt caataacagc
    19981 ttcaaaaaac gttcaaaacc gataacaacc aggctgtaa
//
LOCUS       DAJTYL010000073        20015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYL010000073 DAJTYL010000000
VERSION     DAJTYL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.073"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..409
                     /gene="ygaP"
                     /locus_tag="OO572_002559"
     CDS             <1..409
                     /gene="ygaP"
                     /locus_tag="OO572_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6661524.1"
                     /translation="LAPLSVLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAP
                     AEIFLLEDGIDGWKRAGLPVAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFL
                     LSGFVGAGLLFAGISGFCGMARLLDKMPWNQRA"
     gene            complement(456..860)
                     /gene="stpA"
                     /locus_tag="OO572_002560"
     CDS             complement(456..860)
                     /gene="stpA"
                     /locus_tag="OO572_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6661525.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1528..1977
                     /gene="alaE"
                     /locus_tag="OO572_002561"
     CDS             1528..1977
                     /gene="alaE"
                     /locus_tag="OO572_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6661526.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2014..2358)
                     /locus_tag="OO572_002562"
     CDS             complement(2014..2358)
                     /locus_tag="OO572_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6661527.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2510..2740
                     /locus_tag="OO572_002563"
     CDS             2510..2740
                     /locus_tag="OO572_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661528.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3087..3332
                     /gene="nrdH"
                     /locus_tag="OO572_002564"
     CDS             3087..3332
                     /gene="nrdH"
                     /locus_tag="OO572_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6661529.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3329..3739
                     /gene="nrdI"
                     /locus_tag="OO572_002565"
     CDS             3329..3739
                     /gene="nrdI"
                     /locus_tag="OO572_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6661530.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            3712..5856
                     /gene="nrdE"
                     /locus_tag="OO572_002566"
     CDS             3712..5856
                     /gene="nrdE"
                     /locus_tag="OO572_002566"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6661531.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            5866..6825
                     /gene="nrdF"
                     /locus_tag="OO572_002567"
     CDS             5866..6825
                     /gene="nrdF"
                     /locus_tag="OO572_002567"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6661532.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7180..8382
                     /gene="proV"
                     /locus_tag="OO572_002568"
     CDS             7180..8382
                     /gene="proV"
                     /locus_tag="OO572_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6661533.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8375..9439
                     /gene="proW"
                     /locus_tag="OO572_002569"
     CDS             8375..9439
                     /gene="proW"
                     /locus_tag="OO572_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6661534.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9497..10489
                     /gene="proX"
                     /locus_tag="OO572_002570"
     CDS             9497..10489
                     /gene="proX"
                     /locus_tag="OO572_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6661535.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10681..11860
                     /locus_tag="OO572_002571"
                     /pseudo
     CDS             10681..11860
                     /locus_tag="OO572_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11984..12721
                     /gene="ygaZ"
                     /locus_tag="OO572_002572"
     CDS             11984..12721
                     /gene="ygaZ"
                     /locus_tag="OO572_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6661536.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            12711..13046
                     /gene="ygaH"
                     /locus_tag="OO572_002573"
     CDS             12711..13046
                     /gene="ygaH"
                     /locus_tag="OO572_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6661537.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13137..13667
                     /gene="emrR"
                     /locus_tag="OO572_002574"
     CDS             13137..13667
                     /gene="emrR"
                     /locus_tag="OO572_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6661538.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            13794..14966
                     /gene="emrA"
                     /locus_tag="OO572_002575"
                     /pseudo
     CDS             13794..14966
                     /gene="emrA"
                     /locus_tag="OO572_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            14983..16521
                     /gene="emrB"
                     /locus_tag="OO572_002576"
     CDS             14983..16521
                     /gene="emrB"
                     /locus_tag="OO572_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6661539.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16585..17100)
                     /gene="luxS"
                     /locus_tag="OO572_002577"
     CDS             complement(16585..17100)
                     /gene="luxS"
                     /locus_tag="OO572_002577"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6661540.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17250..18806)
                     /gene="gshA"
                     /locus_tag="OO572_002578"
     CDS             complement(17250..18806)
                     /gene="gshA"
                     /locus_tag="OO572_002578"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6661541.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(18879..19307)
                     /locus_tag="OO572_002579"
     CDS             complement(18879..19307)
                     /locus_tag="OO572_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6661542.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19304..19870)
                     /gene="yqaB"
                     /locus_tag="OO572_002580"
     CDS             complement(19304..19870)
                     /gene="yqaB"
                     /locus_tag="OO572_002580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6661543.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4816 a         5125 c         5009 g         5065 t
ORIGIN      
        1 tctggctcca ttatccgtgc tggaacagtc aggtcttcca cctaaattac gtcgcgagca
       61 aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata aattagcagc
      121 gattgccgcc cccgcagaaa tatttttact cgaagatggg attgatggct ggaaaagagc
      181 tggattgcca gtagcggtaa ataaatctca acccttgccg ttaatgcgcc aggtgcagat
      241 cgctgcgggt ggtttaatat taaacggcgt tgtactgggt tataccgtaa atagcggttt
      301 cttcttatta agtggctttg ttggtgccgg gttactgttt gcaggaatca gcggtttttg
      361 tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc
      421 ggacgcgccc tagcagcgac atccggcctc agtaattaga tcaggaaatc gtcgagagat
      481 ttaccttctg ccagcgcctg agcaattggc ttcggtgtac ggccctgacc ggtccaggtt
      541 ttagtttcac cgttaacatc agtgaattta tatttcgccg gacgcggctg gcgtttttta
      601 ccagcacgtg gtgcagcagc agagctatta cccaataact cttccgggtt aattccgtca
      661 gctttcatca gctccagcca ggtgctaatt ttttcctgac gctcagccag ttcacgctgc
      721 tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc gagcatttct
      781 tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa
      841 ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa aaaagtattt
      901 cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct atttacagtc
      961 ctgcgcgaga ttatttcagc tgttattttc tgaaactctc agaataattt caagattata
     1021 tattagccat tttgatgagt aatttaggtt attggtgaac agagtggcgc tctctgctcg
     1081 ctaactccgc aagcctgaca aaaccgaagt gaataaaaat gcaatataac agaataaaag
     1141 aaaacacccc tcactggtgc tttctgctgt taatgtacat tttctggcat caaaaatgct
     1201 gtaaatgacc cggtcatgac aaacaccgaa ttctctacgc ttatagcgtt acctcccccc
     1261 caaactttca ttagtgaaat taaaggcaag atcatatcta attgatttaa ttgaaaataa
     1321 atcatattgg catgtaaaaa tccacaatgt acaaaaaacg agccgttacg gaatatttta
     1381 tctacaaaaa ctaactaaat aaaaattttt cactaattga ttagtcatag acagcgatat
     1441 acgctatgca aaaatgcaga tggcaatgag atccactgct ttcatctcca ttaacatccc
     1501 attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt gcgtcatgca
     1561 gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt
     1621 ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc gattccggtg
     1681 aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg cgcggcacgc
     1741 aaagttagcc cgtcgggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc
     1801 cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg
     1861 gcggcggtca gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc
     1921 ctcgattatt gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc
     1981 ttcttttcgc gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt
     2041 caaataacgt tcaagcgcaa cacgcgaact aaagccatgc gggataccgt aatgctcgtg
     2101 tcgctcgcct gctaaataga gcggaaattg ctggtaatga tcgcaagttt taatatcgga
     2161 agcgggttca gccagtgttg aactgcgttc ttttaatgtt ggatgaatta ccagagcggt
     2221 acgtcccatt cgcgcttcgc gattaacata gacataattt tccccacggc ggtaaccata
     2281 cgctttctgg gttaccacat cgacagtaaa accgactttt tcaagtacgc gcgccacctc
     2341 gtctggtcgt aaatacatat ttgatcctcg ttattttcaa ccgcgggcct accttacctg
     2401 attgcgcatt cacaacgctt tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga
     2461 aaattatggt ctacactgaa aattacatcg aattctaatg ggggatcata tgtttaaccg
     2521 accgaaccgc aacgacgttg atgatggcgt gcaggatatc cagaatgatg tcaatcaatt
     2581 agctgacagc ctcgaatctg tattgaagtc ctggggcagc gacgccaaag gggaagctga
     2641 agccgcacgc agtaaagctc aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg
     2701 tactcgcgtc cagcaagccg cgcgcgatgc cgttggctga gcggattctt ttgttcgtga
     2761 aagaccctgg tgtagcgtgg gtacagcagc tgcggtaggt atttttatcg gcgcactgtt
     2821 aagcatgcgc aaatcgtagt gcaaaaatga taataaatac gcgtctttga ccccgaagcc
     2881 tgtcttcggg gtttcttttt gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc
     2941 atatccaggg taatttcgat cactatttgc tatatattgt gtagttgaat cttttttcaa
     3001 ctacatctag tatctctgta tcaacagaga gacaacccga cgcgtatcat cgcgccgtat
     3061 cttcatttta aacggaaata cgaatcatgc gcataactat ttatactcgt aacgattgcg
     3121 ttcagtgcca cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta
     3181 atgtcgatcg cgttcctgaa gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc
     3241 cggtagtgat tgctggcgat cttagctggt ctggtttccg tccggacatg attaaccgtc
     3301 tgcatccagc gccacacgcg gccagtgcat gagccagctc gtctacttct ccagcagctc
     3361 cgaaaacacg cagcgtttta tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa
     3421 tgagcgggaa cggattcagg tagacgagcc ttacatcctg atcgtgccct cttacggcgg
     3481 cggcggtacg gctggcgcgg tgccacgaca ggtaattcgc tttttaaacg acgagcacaa
     3541 ccgggcgttg cttcgcggcg ttattgcttc tggtaatcgc aactttggtg aggcgtatgg
     3601 ccgcgccgga gatgtgattg cccggaaatg cggcgtgccg tggctgtacc gttttgaact
     3661 catgggtacg caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg
     3721 acaaccgcag aatgcctgac gcaggaaacg atggattacc acgcgctgaa tgcgatgctt
     3781 aatctttacg atagcgcagg tcgcattcag ttcgataaag accgccaggc cgttgacgcc
     3841 tttattgcga cgcatgtgcg tccgaacagt gtgaccttca gtagccagca gcagcgcctg
     3901 aactggctgg tcaacgaagg ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac
     3961 tttgtcatta cgctgtttgc ccacgcacac accagcggtt ttcgtttcca gacattcctc
     4021 ggggcatgga agttttacac cagctatacg ttgaagacat tcgacggtaa acgttatctg
     4081 gaagattttg ccgatcgagt cacgatggtg gcgctgacgc tggcacaagg cgatgagacg
     4141 ctggcgttgc aactgaccga tgaaatgctg tcaggacgct ttcagccagc cacgccaaca
     4201 ttcctcaact gcggtaagca gcagcgcggc gaactggttt cctgtttttt gctgcgtatt
     4261 gaagacaata tggagtcgat tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc
     4321 ggcggcggcg tagcattttt gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt
     4381 attgaaaatc aatcttctgg cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc
     4441 tatgccaacc aactcggcgc gcgtcagggg gcaggtgcag tctatttaca tgctcatcat
     4501 cccgatattc tgcgttttct cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt
     4561 aaaacactgt cgctgggcgt ggtgatcccg gatatcactt tccatctggc aaaagagaat
     4621 gcgcagatgg cgctgttttc gccctatgac gttgagcgag tttatggcaa gccgtttgcc
     4681 gatatcgcca ttagcgaaca ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa
     4741 tacctcaacg cccgtgattt cttccagcga ctggcagaaa tccagtttga gtccggctat
     4801 ccctacatca tgtatgaaga cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat
     4861 atgagtaatc tctgctcaga aattttgcag gttaacagcg cctcagagta tgacgagaat
     4921 ctcgactatg cccgcacagg ccatgatatt tcctgcaatt taggttcgtt gaatattgcg
     4981 cacaccatgg attcccccga tttttcccgc acggtagaga ctgccgtgcg cggtttaacg
     5041 gcggtgtcag atatgagtca tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc
     5101 tcgcacgcca tcggactggg gcagatgaat ttacatggtt atctggcgag ggaaggcatc
     5161 gcttatggtt cgccggaagc cctggatttc accaatctct atttctatac catcacctgg
     5221 cacgcactgc gtacctcgat gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc
     5281 aaacagtcac gctatgccag tggtgaatat ttcagccaat atctgcaagg taactggcag
     5341 ccgaaaacgg cgaaagttgg cgaactgttt gcccgcagct gtattacgtt acctacccgt
     5401 gagatgtggg cgcaactgcg cgacgacgtg atgcgctacg gcatatacaa ccagaatctc
     5461 caggcggtgc cgccaaccgg gtctatctct tatatcaacc atgctacgtc gagtattcat
     5521 ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa caggacgcgt ttactaccct
     5581 gccccgttta tgactaacga aaatctggcg ctgtatcagg acgcttacga aattggcgca
     5641 gaaaagatca tcgacaccta cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg
     5701 acgctgtttt tccccgatac cgccaccact cgcgatatca acaaagcgca gatttacgcc
     5761 tggcgcaaag gtatcaaaac gctctattac atccgcctgc gtcagatggc gctggaaggc
     5821 actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa actctcacgt
     5881 atcagcgcca tcaactggaa caagatatct gacgataaag atctggaggt gtggaatcgc
     5941 ctgaccagca atttctggct accagaaaag gtgccgctgt cgaacgatat tcccgcctgg
     6001 cagacattaa ctgtcgtaga acaacaactg acgatgcgcg tttttactgg cctgacgctg
     6061 ctcgacacgc tgcaaaatgt tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct
     6121 catgaagagg cggtattatc gaatatcagc tttatggaag cggttcatgc ccgctcttac
     6181 agttcgattt tctcgacgct gtgccagacc aaagatgtcg atgccgccta cgcctggagt
     6241 gaagaaaacg caccgttgca gcgaaaagct cagattattc agcaacatta tcgcggtgat
     6301 gatccgctga aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt ctattccggt
     6361 ttctggctgc cgatgtattt ttccagccgc ggaaagctga ccaataccgc ggacctgatc
     6421 cgtctgatta tccgcgatga agcagtccac ggttactaca taggctataa atatcagaaa
     6481 aacatggaaa agatatctct gggacaacgt gaagagttga agagtttcgc cttcgatttg
     6541 ttgctggaac tctacgacaa cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg
     6601 tgggctgacg atgtgaaagc gtttctctgt tacaacacca ataaggcttt gatgaatctg
     6661 ggctacgaac cgttatttcc cgcagaaatg gcggaagtga atccggcaat cctcgccgcg
     6721 ctttcgccga atgccgatga aaatcacgat ttcttttccg gttcaggctc ctcttatgtg
     6781 atggggaaag cggttgaaac agaagatgaa gactggaatt tctgagggtg ttattttcaa
     6841 aaatatcact acccgcagca gggaaataat tcccgccaaa tagcttttta tcacgcaaat
     6901 attctgtagt gatctacact gatactctgt tgcattattc gcctgaaact acaatattca
     6961 ggcgtttttt cgctatctat gacaaaaaat atcaactttt ctcgatttgc tctcagccct
     7021 tatatcacgg aaaattccgg cgatttgctc gcatcaatat tcatgccaca tttgccctca
     7081 ggggttgcct cagattctca gtatgttagg gtagaaaaaa gtgactattc ccattgggta
     7141 atatatcgac ataaacaaat aaagaaatca ttctattgca tggccattaa attaaaaatt
     7201 aaaaatcttt ataaaatatt tggtgagcat ccacagcgag cgttcaaata tatcgaacaa
     7261 ggactttcaa aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc
     7321 agtctggcca ttgaagaagg cgagatattt gtcatcatgg gattatccgg ctcgggtaaa
     7381 tccacaatgg tacgccttct caatcgcctg attgaaccca cccgcgggca agtgctgatt
     7441 gatggtgtgg atattgccaa aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag
     7501 attgcgatgg tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact
     7561 gcgttcggta tggaattggc cggaattaat gccgaagaac gccgggaaaa agcccttgat
     7621 gcactgcgtc aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc
     7681 gggatgcgtc aacgtgtggg attagcccgc gcgttagcga ttaatccgga tatattatta
     7741 atggacgaag ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg
     7801 gtaaaattac aggcgaaaca tcagcgcacc attgtcttta tttcccacga tcttgatgaa
     7861 gccatgcgta ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc
     7921 acaccggatg aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc
     7981 gttgatatta gtcaggtgtt cagtgcgaaa gatattgccc gccggacacc gaatggctta
     8041 attcgtaaaa ctcctggctt cggcccacgt tcggcactga aattattgca ggatgaagat
     8101 cgcgaatatg gctacgttat cgaacgcggt aataagtttg tcggcgcagt ctccatcgat
     8161 tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg
     8221 ttagcagtcg atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc
     8281 tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg
     8341 ctgctgcgcg ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga
     8401 taccacgcca gcggcggaca gtgccgcgca atccgcagac gcctggggta caccgacgac
     8461 tgcaccggct gacggcggtg gtgctgactg gctgaccagt acgcctgcgc caaacgtcga
     8521 gcattttaat attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac
     8581 tgaagggatc gactgggttg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc
     8641 ggttgattat atcctcaacg gtttccagca attgctgctg ggtatgccag cgccagtggc
     8701 gattatcgtt ttcgctctca tcacctggca gatttccggg gtcggaatgg gcgtggcgac
     8761 gctggtctcg ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct
     8821 ggcgctggtg ttaactgccc tgctgttctg tatcgtcatc ggtttgccgt tggggatatg
     8881 gctggcgaga agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac
     8941 cacgccagcg tttgtttatc tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc
     9001 gggcgtggtg gtgacgatca tctttgcgct gccgccgatt atccgtctga ccattctggg
     9061 gattaaccag gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg
     9121 ccagatgctg ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa
     9181 ccagacgctg atgctggccc tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg
     9241 gctgggtcag atggtacttc gcggtatcgg tcgtctggat atggggcttg ccaccgttgg
     9301 cggcgtcggg attgtgatcc tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg
     9361 cgactcacgc agtcgcggca accgtcgctg gtacaccact ggccctgttg gtctgctgac
     9421 ccgcccattc attaagtaac tctgcacttg cccggtgacg ccgggcatta tcaccctgcc
     9481 aaaaaaagga ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat
     9541 ctctacacaa acttttgctg ccgatctgcc gggcaaaggc attactgtta atccagttca
     9601 gagcaccatc actgaagaaa ccttccagac gctgctggtc agtcgtgcgc tggagaaatt
     9661 aggttatacc gtcaacaaac ccagcgaagt ggattacaac gttggctaca cctcgcttgc
     9721 ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca ctccatgaca acatgtacga
     9781 agctgccggt ggcgataaga aattttatcg tgaaggggta tttgttaacg gcgcagcaca
     9841 gggttacctg atcgataaga aaaccgccga ccaatacaaa atcaccaaca tcgcacaact
     9901 gaaagatccg aagatcgcca aactgttcga taccaacggc gacggtaaag cggatttaac
     9961 cggttgtaac cctggctggg gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga
    10021 actgaccaac accgtgacgc ataatcaggg gaactacgca gcgatgatgg ccgacaccat
    10081 cagtcgctac aaagagggca aaccggtgtt ttattacacc tggacgccgt actgggtgag
    10141 taatgagctg aagccaggga aagatgtggt ctggttgcag gtgccgttct ccgcactgcc
    10201 gggcgataaa aatgccgata ccaaactgcc gaatggcgcg aattacggct tcccggtcag
    10261 caccatgcat atcgttgcca acaaagcctg gaccgagaaa aatccggcgg cagccaaact
    10321 gtttgccatt atgcagttgc cagtggccga tattaacgcc cagaacgcca ttatgcatga
    10381 cggcaaagcc tcagaaggcg atattcaggg ccacgttgat ggctggatca aagcccacca
    10441 gcagcagttc gatggctggg tgaatgaggc gctggcagcg cagaagtaat ttttattcgg
    10501 gcggataagg cgttttcgcc tcatccgccg ctctgtacac aatgcctgat gcgacgctga
    10561 cgcgtcctat catgcctaca aaccacatcg cacatttcaa caagctatct ttcatcccat
    10621 attcatcaac atccgctatt attgatttcc agcttaatca tcacctgatg aacaaaaata
    10681 atgactaaac ctaatcatga gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc
    10741 ggtctggcgg tagccagtaa ctattacgcc cagccattgc tcgacaccat cgcgcgtaac
    10801 ttttcccttt ccgccagttc ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc
    10861 gcaggtctac tgtttcttgt tcccctcggt gatatgtttg aacgccgccg cctgattgtc
    10921 tcgatgacct tactggcagc gggcggtatg ttaatcactg ccagcagtca gtcactggcg
    10981 atgatgatcc tcggtacggc attaaccggt ttattctcag tcgtggcaca aattctggtt
    11041 ccgctggcag cgacgctggc ttcaccggat aaacgcggca aagtggttgg caccattatg
    11101 agcggtctgc tgttggggat attgctggca cgaaccgttg ccgggttact ggcaaacctc
    11161 ggcggctggc gcaccgtctt ttgggttgct tcggtgttaa tggcactgat ggcgctggca
    11221 ttatggcgtg gtctgccaca aatgaaatca gaaacccacc tcaactaccc acagttgttg
    11281 ggttccgttt tcagcatgtt catcagcgat aagatcctgc gcacccgcgc gttgctgggc
    11341 tgcatgacct ttgccaactt cagcattctc tggacctcaa tggccttttt gcttgccgct
    11401 ccacctttta actacagcga tggtgacatt ggtctgtttg ggcttgcggg agctgccgga
    11461 gcgttgggcg ctcgtccggc gggcggtttt gccgacaagg gcaaatcgca ccacaacttt
    11521 cggtctgctt ctgctgttac tttcatggct ggcaatctgg tttggtcaca cttccgtact
    11581 ggcgttgatt atcggcattc tggtgctgga cctcaccgta cagggcgtgc atatcactaa
    11641 ccagacggta atttatcgaa tacatcctga tgcgcgtaat cgcctgaccg caggttacat
    11701 gaccagctac tttattggcg gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca
    11761 tggcggttgg gctggcgttt gtctggctgg cgcgacgatt gccctggtta acttactggt
    11821 ctggtggcga ggttttcatc gtcaggaagc cgcaaattaa gcaatcagca accgttttcg
    11881 tggctttaca cttataaggg tgttaagaag cccatcagtc tgataaggtt aagatattca
    11941 ttcagtctat ttataatatt aacaatcgtt aagcgtacac tctatggaaa gccctactcc
    12001 acagcctgct cctggttcgg cgaccttcat ggaaggatgc aaagacagtt taccgattgt
    12061 tattagttat attccggtgg cctttgcgtt cggtctgaat gcgacccgtc tgggattctc
    12121 tcctctcgaa agcgtttttt tctcctgcat catttatgca ggcgcgagcc agttcgtcat
    12181 taccgcgatg ctggcagccg ggagtagttt gtgggttgct gcactgagcg tcatggcaat
    12241 ggatgttcgc catgttttgt atggcccgtc actgcgtagc cgtattattc agcgtctgca
    12301 aaaatcgaaa accgccctat gggcgtttgg cctgacggat gaggtttttg ccgccgccac
    12361 cgcaaaactg gtacgcaata atcgccgctg gagcgagaac tggatgatcg gcattgcctt
    12421 cagttcatgg tcatcgtggg tatttggtac ggtaataggg gcattctccg gcagcggctt
    12481 gctgcaaggt tatcccgccg ttgaagctgc attaggtttt atgcttccgg cactctttat
    12541 gagtttcctg ctcgcctctt tccagcgcaa acaatctctt tgcgttaccg cagctttagt
    12601 tggtgccctt gcaggcgtaa cgctattttc tattcccgtc gccattctgg caggcattgt
    12661 ctgtggctgc ctcactgcgt taatccaggc attctggcaa ggagcgcccg atgagctatg
    12721 aggttctgct gcttgggtta ctggttggcg cggcgaatta ttgcttccgc tatttgccgc
    12781 tgcgcctgcg tgtgggtaat gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg
    12841 acaccattgg catcgcctcg atatgcgctc tgctggttgt ctctaccgca ccagaagtga
    12901 tgcacgatac acgccgtttc gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt
    12961 tctataaaac acgcagcatt atcatcccaa cactgcttag tgcgctggcc tatgggctcg
    13021 cctggaaagt gatggcgatt atataaccca caagaatcat ttttctaaaa caatacattt
    13081 actttatttg tcactgtcgt tactatatcg gctgaaatta atgaggtcat acccaaatgg
    13141 atagttcgtt tacgcccatt gaacaaatgc taaaatttcg cgccagccgc cacgaagatt
    13201 ttccttatca ggagatcctt ctgactcgtc tttgcatgca catgcaaagc aagctgctgg
    13261 agaaccgcaa taaaatgctg aaggctcagg ggattaacga gacgttgttt atggcgttga
    13321 ttacgctgga gtctcaggaa aaccacagta ttcagccttc tgaattaagt tgtgctcttg
    13381 gatcatcccg taccaacgcg acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg
    13441 aacgtcgtaa aagcgataac gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc
    13501 acgagttttt gcgcgaggtt ttaccaccgc agcataactg cctgcatcaa ctctggtccg
    13561 cgctcagcac aacagaaaaa gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc
    13621 tcgaccagat ggaacaagac ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc
    13681 tcaaaaatcc agatttataa aagaaaaaat gaccggccag catcgcaaca tgctggcctt
    13741 tttggcaagc aggtcggctc agccgatgag ttaagaagat cgtggagaac aatatgagcg
    13801 caaatgcgga gactcaaacc ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc
    13861 tgctcctcct tctcaccttg ctctttataa ttattgccgt agcgataggg atttattggt
    13921 ttttggtact gcgtcatttc gaagaaaccg atgacgcata cgtggcaggg aatcagattc
    13981 aaattatgtc tcaggtgtct ggcagcgtga cgaaagtctg ggccgataac accgattttg
    14041 taaaagaagg cgatgttctg gtcactctcg acccgacaga tgctcgccag gcgtttgaaa
    14101 aagccaaaac tgcactggct tccagcgttc gccaaaccca ccagctgatg attaacagca
    14161 aacagttgca ggcgaatatt gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact
    14221 acaaccgccg tgtgccgctg ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg
    14281 cccgcgacgc cgtcaccagt gcccaggcgc aactggacgt cgctattcaa caatacaatg
    14341 ccaatcaggc gatgattctg gggactaaac tggaagatca gcctgccgtg caacaggctg
    14401 ccaccgaagt acgtaacgcc tagctggcgc tggagcgtac ccgtattgtc agtccgatga
    14461 ccggttatgt ttcccgtcgt gccgtgcaac cgggtgcgca aattagccca acgacgccgc
    14521 tgatggcggt cgttccagcc accaatatgt gggtggatgc caactttaaa gagacgcaga
    14581 ttgccaatat gcgcatcggt cagccggtca ccatcaccac cgatatttac ggcgatgatg
    14641 tgaaatacac cggtaaagtg gttggtctgg atatgggcac aggtagcgcg ttctcactgc
    14701 ttccggcgca aaatgcgacc ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta
    14761 tcgaactgga ccagaaacag ctggagcaat acccgctgcg cattggtttg tccacgctgg
    14821 tgagcgtcaa taccactaac cgtgacggtc aggtactggc aaataaagta cgttcaacac
    14881 cggtcgcggt aagtaccgcg cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg
    14941 atatcgtaaa agctaacgct ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg
    15001 ctggaaggcg cgcaactggt cattatgacg attgcgctgt cactggcgac attcatgcag
    15061 gtgctgaact ccaccattgc taacgtggcg atccccacta tcgccgggaa tctgggctca
    15121 tcgctcagcc aaggaacgtg ggtaatcact tctttcgggg tggcgaatgc catctcgatc
    15181 ccgcttaccg gctggctggc aaagcgcgtc ggggaagtga aactgttcct ttggtccacc
    15241 atcgcctttg ctattgcgtc ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc
    15301 ttcttccgcg tgattcaggg gattgtcgcc gggccgttga tcccgctttc gcaaagtcta
    15361 ttgctgaata actacccgcc agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg
    15421 gtgattgtcg cgccaatttg cggcccgatc ctcggcggtt atatcagcga taattaccac
    15481 tggggctgga tattcttcat caacgtgccg attggcgtgg cggtggtgtt gatgacactg
    15541 caaaccctgc gcggacgtga aacccgcacc gaacggcggc ggattgatgc cgtggggctg
    15601 gcactgctgg ttattggtat cggcagcctg cagattatgc tcgaccgcgg taaagagctg
    15661 gactgttttt catcacagga aattatcatt cttaccgtgg tggcggtggt ggctatctgc
    15721 ttcctgattg tctgggagct gaccgacgat aacccgatag tcgatctgtc gttgtttaag
    15781 tcgcgcaact tcaccatcgg ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc
    15841 gctattgttc tgctgccgca gttgttgcag gaggtctacg gttacacggc gacctgggca
    15901 ggtttggcct ctgcgccggt agggattatt ccggtgattc tgtcgccgat tatcggccgc
    15961 ttcgcgcata aactggatat gcggcggctg gtaaccttca gctttattat gtatgccgtc
    16021 tgcttctact ggcgtgccta tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg
    16081 ccgcagttta tccaggggtt tgcggtggtc tgcttcttta tgccgctgac caccattacg
    16141 ctgtctggtt tgccaccgga acgactggcg gcggcatcga gcctctctaa ctttacgcga
    16201 acgctggcgg ggtctatcgg cacgtcgata accacgacca tgtggaccaa ccgcgagtcg
    16261 atgcaccatg cgcagttgac tgagtcggta aacccgttca acccgaatgc ccaggcgatg
    16321 tacagtcaac tggaagggct tgggatgacg caacagcagg catcaggctg gattgcccag
    16381 cagatcacca atcaggggct gattatttcc gccaatgaga tcttctggat gtcagccggg
    16441 atattcctcg tactgctggg gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc
    16501 ggcggaggcg gtgcgcacta agtacaacta agccagttca tttgaactgg cttttttcaa
    16561 ttaattgtga agatagttta ctgactagat gtgcagttcc tgcaacttct ctttcggcag
    16621 tgccagttct tcgttgctgt tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc
    16681 ctgcgcttcc tgcaacgagt gcatctggta agtgccacac tggtagacgt tcagttccgg
    16741 gatctgattc tgatcctgca ctttcagcac gtcttccatt gccgctttcc aggcatcagc
    16801 aacacgctgc tcatctggcg taccaatcag actcatataa aaaccggtgc ggcagcccat
    16861 tggcgagata tcgataatct ctacaccatt accgttaaga tggttacgca taaaaccagc
    16921 aaacaggtgc tccagggtat ggatccctct ttctggcatc acttctttgt tcggcacgca
    16981 gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg gtgttcattg ttttcgccac
    17041 ccgaactgca ggcgcttcca tccgggtatg atcgactgtg aagctatcta acaacggcat
    17101 ttagccacct ccggtaattt ttttaaaaat tttctgaact ctttcttccc aggcgagtct
    17161 gagtatatga aagacgcgca tttgttatca tcatccctga attcagagat gaaattttgg
    17221 ccactcccga gtggcctttt tcttttctgt caggcgtgtt tttccagcca caccgcaaac
    17281 ggttcggtat cagcggtttc catttcctgc tgacggcgtt cagatgcctc gcgctcggct
    17341 acaaaatcct cttcgcgcag aatttccagc ggctcttcac gcagcagatt acggtaggct
    17401 tctgcaaacg ctttgcctgt tccgccaata ccagtatcaa tcatagacct taagatacgg
    17461 gcagagaaag tcagatcggg attatcgaag caggcaacca gttcatcaca cactttctga
    17521 tacgcttcgc cgccgttaat gctatccagc gtttgcgcga cgcgtttcag atcgcggaac
    17581 agatctttac ccacctgcag taacgggaac tgcgcggttt cgcagccgat acccagcgtc
    17641 aggcccggtt tgcgaccttc aaggatcacc cggttccagt taacgcgcgt acaggcaagt
    17701 tcgctactgc tcatttccgg tgcatcagcc agcgcacacc agaccataaa caggtcgagg
    17761 aatcgcacct gctgttcatc tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc
    17821 acttcaatat attcaatgcc gccacgtaac agcgcatcag aaggtgactc gccgctgcgg
    17881 gtaacgcgtt ttggacgaat cggcgcatac agttcgtttt caatctgcag cacgttgcta
    17941 ttgatttgca gcctcttacc gtctttctca ataccaatct tcgcgtactc ttccgatggc
    18001 gttttgattg cctgtttaag gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca
    18061 agattgcttt gcgatttatt ggtatagccg agatcgctca aacgaagaga ggtcgcatac
    18121 ggcaggtaat acataccgca ctcggttttc tcaaacggca gcgacgttgg ttttccttgc
    18181 aggaaagaag aacaaatcgc cggagatgca ccaaacagat aaggaataac ccaaccgaaa
    18241 cgatagtaat tgcggataac gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg
    18301 cccgagatat caccgcactt cgcttgccag aatgccattg gcaaagagaa attgtagtgc
    18361 acgccggaaa tggtttgcat cagcgcgccg tagcgatttt tcagcccttc acgatacagc
    18421 gttttaaagc gtccggtgtt agaagtgccg tactgtgcca gttcgatgtc ctgaccttct
    18481 gcgatgtagc atggcatact taacggccac atccgctcat cgcccatatt gcgcgccgta
    18541 taacgatgca gatcgcgcat aaaggtcagc atatgttcaa tatcaccatc cactggtgta
    18601 atgaattcca gcaatgcttc cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa
    18661 cctaatgctt caggatgacc tgttgttgcc agtgtgccat cagcattaac acgcaaagtt
    18721 tcgcgctcca gcccacgctg tatccccttt aacgcctgag gatgtttttc cagccaggcc
    18781 agcgcctgtg atacgtccgg gatcaaattg acctcccgcc tgtcaaaatc gttttaatta
    18841 gcataactgt aatggtgacc atatgtgcag gcctacaatt agtgccacca catcatgccc
    18901 tgaacggtcg ctgctgcaac tgcaacatag cgtaacgctt taccaaggca caaaaaaaag
    18961 attactggcc cccaggagat gcgcatccat cccgctaaca gacacagtaa atcgccaacc
    19021 acgggcatcc agcttaataa tagcgtgact gcaccatagc gtttcagcca gccggtagct
    19081 ttctcttgcc agcgcgatgt tttacgcaat ggaaagaaac gcccaaggat aacgttagtt
    19141 aaccctccaa ggctattacc cattgttgct gttaaaacta aaacccaggg atgactgatc
    19201 ccggaaagca acattgccac cagcacgact tcggagttgc cgggtaatag tgtagcgctg
    19261 agaaaactac tggcaaacaa cgagaaaagc gataacgctt cactcacagc aagcgaacat
    19321 ccacagcgtc catgcctgct gcacgggccg cctgaatacc gaaatcggca tcttcaaaga
    19381 ccacacactg cgtcggttgc acgcccatac gctgcgcgca caacaaaaat gtgtctggcg
    19441 cgggtttatg gtgtttgacg tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc
    19501 ccaggtgcgc cagcaatgcc tcagcgatgg cgctttcact ccccgttcct acagccattg
    19561 ggcgacgacc atgccaactt ttcaccactt caacaagagg aagcggctcg acactatcca
    19621 gcagcatact tcttactgct tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg
    19681 cctgattcag ctcaataatt gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa
    19741 tcatcgcctg aatatcgtac tgaagaccgt agtgccctaa tacttcgcgc cacgctttac
    19801 ggtgcgtagg cgccgtatcc aggattgtgc catccatatc aaaaattaaa cctgcataac
    19861 gctcgtacat ggtcttctcg caagtcgaaa aatatgacgc tactttagcg taattgcttg
    19921 attttgtcgc tgatggcgaa ggagaagaaa attcaggaaa tggataaagt agttatttag
    19981 aaatgagata tttttgaagg aaattttttg gagaa
//
LOCUS       DAJTYL010000074        19962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYL010000074 DAJTYL010000000
VERSION     DAJTYL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.074"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..367
                     /locus_tag="OO572_002581"
     CDS             <1..367
                     /locus_tag="OO572_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6661544.1"
                     /translation="AHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAF
                     AFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKP
                     EAVQEALAKVIPFFNTHLG"
     gene            385..1023
                     /locus_tag="OO572_002582"
     CDS             385..1023
                     /locus_tag="OO572_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6661545.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPLKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1314..2042
                     /gene="mpaA"
                     /locus_tag="OO572_002583"
     CDS             1314..2042
                     /gene="mpaA"
                     /locus_tag="OO572_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6661546.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2017..2982)
                     /gene="ycjG"
                     /locus_tag="OO572_002584"
     CDS             complement(2017..2982)
                     /gene="ycjG"
                     /locus_tag="OO572_002584"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6661547.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3101..3607
                     /gene="tpx"
                     /locus_tag="OO572_002585"
     CDS             3101..3607
                     /gene="tpx"
                     /locus_tag="OO572_002585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6661548.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3651..5192)
                     /gene="tyrR"
                     /locus_tag="OO572_002586"
     CDS             complement(3651..5192)
                     /gene="tyrR"
                     /locus_tag="OO572_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6661549.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5340..6401)
                     /locus_tag="OO572_002587"
     CDS             complement(5340..6401)
                     /locus_tag="OO572_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6661550.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6398..7795)
                     /locus_tag="OO572_002588"
     CDS             complement(6398..7795)
                     /locus_tag="OO572_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6661551.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7951..8949
                     /locus_tag="OO572_002589"
     CDS             7951..8949
                     /locus_tag="OO572_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6661552.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9060..9932)
                     /gene="ompG"
                     /locus_tag="OO572_002590"
                     /pseudo
     CDS             complement(9060..9932)
                     /gene="ompG"
                     /locus_tag="OO572_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10005..11086)
                     /locus_tag="OO572_002591"
                     /pseudo
     CDS             complement(10005..11086)
                     /locus_tag="OO572_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11100..11759)
                     /gene="pgmB"
                     /locus_tag="OO572_002592"
     CDS             complement(11100..11759)
                     /gene="pgmB"
                     /locus_tag="OO572_002592"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6661553.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11756..14023)
                     /gene="ycjT"
                     /locus_tag="OO572_002593"
     CDS             complement(11756..14023)
                     /gene="ycjT"
                     /locus_tag="OO572_002593"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6661554.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14020..14643)
                     /locus_tag="OO572_002594"
     CDS             complement(14020..14643)
                     /locus_tag="OO572_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6661555.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14640..15062)
                     /locus_tag="OO572_002595"
     CDS             complement(14640..15062)
                     /locus_tag="OO572_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6661556.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15084..15859)
                     /locus_tag="OO572_002596"
                     /pseudo
     CDS             complement(15084..15859)
                     /locus_tag="OO572_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(15877..16929)
                     /locus_tag="OO572_002597"
     CDS             complement(15877..16929)
                     /locus_tag="OO572_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6661557.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(16960..17802)
                     /locus_tag="OO572_002598"
     CDS             complement(16960..17802)
                     /locus_tag="OO572_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6661558.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17789..18670)
                     /locus_tag="OO572_002599"
     CDS             complement(17789..18670)
                     /locus_tag="OO572_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6661559.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18691..19956)
                     /locus_tag="OO572_002600"
                     /pseudo
     CDS             complement(18691..19956)
                     /locus_tag="OO572_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         4839 a         5494 c         4884 g         4745 t
ORIGIN      
        1 agcccatgga ctgaccgaac gcgatgttta tgaagcaact gactactata aaacaccacg
       61 cgggcaacaa ccgggaggtg cgacaaaaat gttgttctca catgcacaga aaacgctggc
      121 ctgggatgcc ttcgcattta ctgaagtgtt actgacccag ccggtgatgg tagtagttgg
      181 tgaaaaagtc ggtgccttcg gtgcttatcg tgatggactg gaagtctatg gccgggcgat
      241 ggtttcgcag gatcgtcagt tagtttcgtt acccgatttc tcacattatg aactatatga
      301 caaaccggaa gcagttcagg aggcgctggc aaaagttatt ccttttttca acacccatct
      361 gggataagtt tcatgagaaa tattatgaaa aatgtactga ttcttggtgc tggcggtcag
      421 atagcccgcc atgtgattaa tcaactggca gataaacaaa ctattaaaca aactttattt
      481 gcccgtcagc cagcaaaaat acacaaaccc tatccaacta atagccagat tattatgggg
      541 gatgtactga atcatgcagc actgaagcag gccatgcagg gacaggatat tgtgtatgcc
      601 aaccttacgg gggaagattt agatattcag gcaaattcag tgatcgctgc gatgaaagcc
      661 tgtgatgtta aacgtctgat ctttgttctt tccctcggta tttatgatga agttccagga
      721 aaatttgtcg aatggaataa tgcagttatt ggtgaacccc taaaaccatt ccgcagggct
      781 gcggatgcaa ttgaggcttc tggactggaa tatacaattc ttcgccctgc ctggctgact
      841 gatgaagata ttattgatta tgagcttacc tctcgcaatg aaccactcaa aggtacgatc
      901 gtatcacgta agagtgtcgc agcattaatt acagatatca tcgataaacc tgagaaacac
      961 attggtgaga atataggcat caatcaaccc gggactgatg gcgataagcc attcttcatg
     1021 taaatcaatt atatagatac catcgtactg tactatgtgc agtacgattt attaaactta
     1081 ttaaggtgaa ctgatcaatc agatgcgatt aacatgtgta gaaccagtaa gaataaaata
     1141 cgccatgtat ataacattac gacaattcta atatctgcac atcaatgaaa atgcaaacaa
     1201 cttccggcaa aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa
     1261 tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg
     1321 taacccgctc acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt
     1381 cattattggg cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt
     1441 tgattctggc tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc
     1501 tgcggacttt gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg
     1561 gctgccagct ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg
     1621 cggcgaactg gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg
     1681 atgtcgtatt actgacaggt gacaaacctg gctccgaacc tgaaacccag gcgctgtgcc
     1741 agcttataca ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcttgta
     1801 ttgaagatcc cagacacaac gaattaggcg aatggctggc ccagtcgttt gaattgcctc
     1861 tggtaaccag cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca
     1921 acttgcattg catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat
     1981 atctctttgc gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt
     2041 gaactgaagc gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa
     2101 actgacctgc ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca
     2161 gcccagcatc agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt
     2221 cagaccaccg gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt
     2281 cagattgcta cgagtatgac aactttcatc agcacaaatc ggcaacggat gaataaaatt
     2341 ctccagcgcc gcatcgtcct gcgcaggaag cggttgttca agcatcgcaa cgcctaaatc
     2401 cgccaatagc tggcaacgcg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac
     2461 gatcagcgtg gcatcgggca cagcggtgcg aattgccacc atccgctcac tgataagatg
     2521 gttatccagc ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt
     2581 ggccatctga tcaggcgtac cgatgacaac cgtctgtgca gtgataactg tctcgggaag
     2641 cgttatgccg atcaaatcag ccagcgattg ctgctgtttt cgcgccgcga gatcccacaa
     2701 tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg
     2761 tgtcagccct ttctctaatt gcggcacgac gctcataatt tgcgccatta ccgaggcatc
     2821 actttctcca taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc
     2881 cagttcaacc accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt
     2941 atgtaagggc caggcttcct caaatacctt aacgattctc atctttactc cttgttgtcg
     3001 cggtggtctg cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac
     3061 taggtgtgac gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt
     3121 ccagggcaac ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac
     3181 ttttactctc gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg
     3241 caaagtgctg aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa
     3301 gtttaaccaa ctggcaactg agatcgacaa caccgttgtg ctgtgtatct ctgccgatct
     3361 gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc
     3421 cactttccgt aacgctgaat ttctgcaagc ctacggtgtg gcaattgctg atggcccact
     3481 gaaaggtctg gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag
     3541 ccagctggtg gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa
     3601 agcataagtt aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg
     3661 ttcttcttct gactcagacc atattcccgc aacttattgg caatcgcggt atgtgaaacg
     3721 ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc
     3781 gagcgttcaa aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg
     3841 gctaccgttg cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg
     3901 tccagttgtg tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac
     3961 gcataacgtg taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc
     4021 tcgtcggcaa agcgggcgac gaacagctca gttaacggca taatgtcctg cggacagtca
     4081 cgtagcggcg gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac
     4141 acgccttttt gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg
     4201 acatgcacct catggtcttc gccaacccga cggaaagtgc catcattaag aaaacgcagt
     4261 aatttcgcct gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg
     4321 ttcgcctgct caaagaaccc tttcttccct tccggagcat gaccaaacag ttcactctcg
     4381 accgcatctt ctggtataga cgcgcaattc agcgccaggt aaggtttgcc cgctctgggg
     4441 cttgcctgat ggcaggcgta ggcaaaaaga tctttacctg tacctgtgtc acccgtaatc
     4501 agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt cgacaacgtg cttcattttc
     4561 gggctgacgg cgacaatttg gctgaaggcg ctgacgtcct gggcggcgac attttgcaac
     4621 tggcggccca tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga
     4681 tcattttcat cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata
     4741 acgacatgct cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag
     4801 ccgttaatca attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc
     4861 tgacagctcg ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt
     4921 tcaggcaacg cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac
     4981 gggacagtac gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg
     5041 aaactctcaa actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca
     5101 ataccgcgta aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg
     5161 agtcggtctt cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat
     5221 taatatgatg atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca
     5281 caaagacgga aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat
     5341 tatttttcgc tgggcgtttt gcctttttgc agagtttctt tcacctgacc gataagctga
     5401 cgacggaaat ccccaaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc
     5461 tccatagctt taatcccgag tcgtaccgtc agaagtccag caccaattcc ctgagctgcg
     5521 cgggtagaca aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc
     5581 agttcgctgg ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg
     5641 ctgtaatacc ccagttcaat gccatacagc gtggcaatgc gattaattaa acgcagattg
     5701 cgccaggcga taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt
     5761 gattccgccg ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc
     5821 aaatgcgcat acaggctgac cacttcacga tcgttctgcg tttcatggat tgaggcatac
     5881 cagcgttgca gcgccggatg cgactgatca atacccgcct gctgcgccag tttttcgcaa
     5941 aatgcgcgac ctttgcccgt gccgtggcta tgcaataaat cacgcgcttc gtcgcgttca
     6001 tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca
     6061 gcgccgataa tcaatgcccc agcggcacat ccacccagcg ctacccagtc ctgggtttgc
     6121 caggcattca ttgtccactg tataccctgc ccgacaacgc ttgcgccaaa cagagccagc
     6181 ccgcccatca ccattttgcg ccacaggctg cgtttcggac gtaacgctgc gtccattacc
     6241 gcttcgactt gcccctcttc ttcctgcgct tcgtcgagcg tggccggggc aaaattttgc
     6301 gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg gattctgttc gacctccagc
     6361 ggaccgtcga aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta
     6421 aaaattccag cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct
     6481 gcgggcgaaa cgcttcaaac tggaaccctt gcttgtccca gaacgcctga ccgggcaaac
     6541 gcgcaggaac ttcaccgggg taaacggtga gaagcgcacc atcgctgagc cggttaccgc
     6601 gcaatgccgg aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg
     6661 aggccagccc caggcaatcc atactaatcc tttcaaacgc cgcattttgc caggcatcct
     6721 gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg atcgaccgtt acatggtccg
     6781 ctttcatcgc ggcaaacagc agtttgtcga taaccggcga gaataagcgg cgaaacagcg
     6841 tgcgctgccc gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa
     6901 atgcctgtgg cccactgttg agaggttgca ggcaatccac cagcacaatc tggcggtcaa
     6961 agcgcagaaa atgattctta tagaacccct tcaccacttt ctcgcagtaa taattaaacc
     7021 gctcgcgcag cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgtccc
     7081 aggcatcgac atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcccg
     7141 gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg ctgtttacag tggtggagat
     7201 aatcggtcca cacggcggca atgtctgcca gacggttttc gtcggcaggc gctagcgggt
     7261 caagtccttc gcacatcatt cgccatttcg ccgaccattc gccgcgctga ccattgagta
     7321 agcccgtcat ctggcgcgac cagcttaagt aatcctgcgc cagcatcggc aggtcgagca
     7381 accattcgcc gggataatcc acaatttcca gatacagcgt ggaggtatcc ttaaagtggc
     7441 gcagcagcgc atcgttcgat ttaaaacgta acgccaggcg aatttcactg acgccgcgcg
     7501 taggcgtcgg ccaggcggga ggatcaccat ataactgcgc cagtccttca tcataggtga
     7561 agcgcggaat gccaaagtca cgctggggaa tgcgttttac gcccagcaag cgctcttcac
     7621 gcaccgcgct taacagcggc aaacgtgctc cggcatgaat attgagcaac tgattaacca
     7681 tcgcggtgat aaacgctgtt ttgccgctgc ggctaagtcc ggttacagcg aggcgcagat
     7741 gtctgtcgac accccgattc accagcgcat tgagttcatt tttaagtcgc ttcattgctg
     7801 tcctgtgctg ctctgaagac cagaaaaaaa atcattcgta aggatatgaa tgtgggggcc
     7861 aggccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat aagatgtgca
     7921 gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga tattgccagg
     7981 gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac taatatctcc
     8041 ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca gccaaacaag
     8101 ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc tacccgttcg
     8161 accaaaacta cggcgggtaa tccgtttttc tctgaagttt tgcatgccat caccgccaaa
     8221 gcggaagaag aaggtttcga cgtgatatta caaacgtcgc acaatccggc agaagatctg
     8281 caaaaatgcg aaagcaaaat taaacagaaa atgattaaag gcattattat gctcagttcg
     8341 ccagcggatg agtcattttt tgcccaactc gataaatacg atattccggt ggtggtaatt
     8401 ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa ttttggtgac
     8461 agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc ctgcctgcat
     8521 gctccgcttg atgtccatgt ttcggttgat cgcgttaatg gctataagca aagtctgtcc
     8581 gcgcataata ttgcagtgcg tgataaatgg attgttgacg gcggttatac ccatgaaaca
     8641 gcactgcaag cagcacggca attattaagt cagtcaccgt tgcctgaggc agtgtttgcc
     8701 actgacagcc tgaaattaat gagcatttat cgtgcggcgg cagagaaaaa tattgctatt
     8761 ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat tttaacgcct
     8821 gcaccaggcg gcatcgatgt tccgacgcag gagttaggtc aacaaagctg cgagttatta
     8881 ttccgcttaa tttcgggaaa accgtcacca cagaatatta ccgttgccac gcatatgacg
     8941 ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctggcg catcttatcc
     9001 ggcctacatt tatagatcgg ataaagtact cgcgccgcat ccggcaattt agcccattat
     9061 cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc gccttcgtcg
     9121 tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gtccgttctg gaaatcataa
     9181 ccgtaaaata aacccacacg gttaaaatca tggccttcac gttcaatatc atcctgccag
     9241 tcccagttac tctagcgatc cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg
     9301 agcgtcagcg gcaaataggc gcgaatttct tgtgtggaaa actcaccgtt attgcggctg
     9361 tcgtccatat tgaagccgcg ctcgagataa tagttcactc gcaaagcaac cgtttcgttg
     9421 aaggtatatt gcagacctgt ttccgtttcg acacgggtat cagcgtaacc ggtcgtgttc
     9481 aaatcgttgg caaatttata catcgacaac caaccgttga aacgtaaatc atcagtcagt
     9541 ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc tttgcccggt
     9601 tcatcaacgt agtgataacc ataattacgg aaaccgccgg tcaggccgaa actgaaatca
     9661 tcgctttcga ggaactgata atgcacctcc agctccgggc gatcaaacct cgttccacgt
     9721 ttacccgcac tataatctac cggcccttcc tgataatagg ccagagcaat tctccacggc
     9781 ccgttggcgg cattaaaata gactgaaggc tccgccagcc catccatatc ttcgccataa
     9841 ccctcgacgt tttctatttc gtacatcgca ccgatattaa agtgccagtc gctcttttcc
     9901 tcggcctgtg cgcaggccat tcccgcacac atcaccagtg cggtacaggg taataacttt
     9961 ttttgttcct tgtattatcc tgggggaatg ccttccccct gtatttagcg aattgctatt
    10021 tccgtttctg catcaaagaa atgacatttc gtcatatcaa aatgaatagt gatattttct
    10081 cctgcatgat aatcatttaa cgcaccagca cggaccacta actcgtgccc ccaaccgtgg
    10141 tgtagagcat aaattccgca ccggttaatt ctgccacgct aattttggcg gaaatgttat
    10201 tttcctcttg cgcgtccgga tgaatatctt ccgggcgtat tcccatcacg atgggcttat
    10261 gcaaactttc ctgtgttttc agaaccgcta atttctcttc gagaatggtt aatttaagcg
    10321 tttccgtaac gaatttatcg ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc
    10381 caataaatcc ggcaacaaac atattcgcgg gttggttata aacggttttc ggcgcaccta
    10441 cttgctgaac aatcccgtct ttcataatca caatccgcgc cgccatggtc atcgcttcgg
    10501 tctgatcgtg ggtcacgtag atcatggtgg tgttcagttt ctgatgcagc ttgctgatct
    10561 ctgcacgcat ttgtacgcgc agcttggcat caaggttaga gagcggttca tccattaaaa
    10621 acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg
    10681 aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc gccgcccagt
    10741 tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca aacgccatgt
    10801 tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc gcgatattgc
    10861 gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg ccgccgctga
    10921 tctcctcaag cccggcaatc atgcgcaggg tggtcgattt accgcagccc gacgggccga
    10981 caaatacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc acatgcacct
    11041 ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg attcctttgc
    11101 tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt tgagggcaac
    11161 agtaattgcg ccccggttaa gcccgcaccg atccccaccg agcgcatacc gctggcgtta
    11221 atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg cggcacgccc
    11281 agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt aagttgggaa
    11341 gcatccgcgc agaaggtgaa aaactcacgc agctccagcg ccgctaaaat cgtcggcgca
    11401 ttcagggaga cggaagccag ccctaccgga atctgctgtg cacggagctc tgccagcaaa
    11461 ttgcgaatac cgggcagcac agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc
    11521 agatttttgc gatacgccag ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc
    11581 ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag agattcgtta
    11641 aactgcgcat caatactgat gccaatttca gcggcaatct gctgccacgc ctggaaatgc
    11701 agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg cagtttcatt
    11761 catcctcctg atgcgtggta gcggtcccat tgaagggcaa aataaaatca cccaaacaga
    11821 aaacagattc ttcagccacg gatataagct gaccgtccaa acgcagtgaa acgggcgcag
    11881 aagttcgaat cgcaatacgc tgcgcgtcga gagtgacctg taattcgcag ccctgccaga
    11941 acagagggaa agacaactgt tgccactgct caagtaacgc cggatttaga tgcaattcac
    12001 cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata gcgccagttg
    12061 cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg atttcagtcc
    12121 cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcagca acaatgccgt
    12181 ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg
    12241 caagacacga tgccgatgag aactgctctg gcagcatgta attgagcatc accacatcag
    12301 cttgtttgag gatctgcatc tcgttcactt ctgcgcgtga ataatccagc agaatggttt
    12361 gcttccccgc cgccgctttg tatttcgcca gattaatcgc cggcttagtc ataaacgaat
    12421 catcctgcgg caaaacaccg tcgggctgaa tttctggcag ccgcagttct ttaaggaaca
    12481 tttcggcacg atgaataaac gcgtcgtcac tacagccaaa ctggcgggca atactcagcg
    12541 cctgctggac gttgtaatac accatatagc tggtgaaggc gttattattg acatgttcgg
    12601 tatattcgtc tggcccaata acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc
    12661 ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc gcaatgaaac
    12721 tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg gccaccagat
    12781 gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg
    12841 caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct
    12901 gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag cgataacgca
    12961 gtaaacttcg ggcaaccgtc ggatcgctaa acagatggaa cggcagcaaa aatacttctg
    13021 tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc gcaatactgc
    13081 tgcgctcgcc gtgagccggc gtcatgatgc gcagatgata aagcgcataa tctaacgctt
    13141 gctgatcgtg cgcatcgccg ccatttaccg tgatacggcg tttctgccac cattgacgcc
    13201 agttttctgt tgatgctgca agatgttggt cgtaactctg ttgcgcgcac atttcaagct
    13261 ggcgaagcga cacgctgccc cactcgtcta aagcagcttg cctgtcatcc cgccagtcga
    13321 tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgta ctggtatgtt
    13381 gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc acctggcagc
    13441 aacaactgat ggccacatca ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat
    13501 gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg
    13561 tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc ataatagtga
    13621 tttccagcgc aatgagcggc agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt
    13681 aacctgcgcc gttgctggta cgccagacaa cgctacggcg taattctcca ctggcaaagt
    13741 caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt atggcaatct
    13801 ccatccccaa gatatcaggc agattcacca gttcgttgat ttcacctttt cccgcccgat
    13861 gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct tcatggctgg
    13921 cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg ttcagggtat
    13981 gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca cacgcgtccc
    14041 tgtttctgcg gattgatata acgccgccac cagttgctgg atgatgtacc cctgctcggc
    14101 gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca tgctgcgcaa
    14161 atggcggttg tcgtctgcca tttcccgttg catcagcgtc attaattcac cgttgttatc
    14221 ggtgtagata tgtgctggaa acagcgtcgc accggcttta tcaccacaaa agctgacgtt
    14281 cataatcaac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc
    14341 gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg ccggatccca
    14401 ctcgccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaaactat gcgcattcac
    14461 gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat gaatgccgat
    14521 gtcgatcagc aggccaccac cctgcagttc tttattggta aagacacccc agccgggaac
    14581 gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa cgccgttggt
    14641 cacctgttca cgcagctgtt gcgtatcgag cgcaaacgat ggtgaaagtc gtaggccagc
    14701 accttgccca gtttgcgcgc ggtatcgcac atttcccacg cctgttctgt cgtcatggcg
    14761 ggcggttttt cgcacatcac atggcagcct gcttccagtg ccatcagggt atgttcgtaa
    14821 tgaaaacggt taggtgagcc gacgctaacc acatcaggtt tcaccgccag cagcatggcc
    14881 tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg cgcctgggaa
    14941 aggtggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta gtacgaagca
    15001 tgaaccttat ccgccacctg gcccgcgcct attatcgcga cccgcagcgg agagcttgtc
    15061 attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga atcacggtac
    15121 gcctgggcag gatcttctgc ccggatacgc ccttcataca ccacataacc ctgatagtta
    15181 tcggcgcgca gctgttcaaa cagcgcgtgg aaatcgaggg tgccactgcc cggctggtaa
    15241 cgatggttat cggcaatatg cacatgaccg agcaagtcac ggttgtcgtg cagcgcctgc
    15301 gccaggttat cttcttcgat gttcatgtga tagaaatcgc cgataatctg cacatgttta
    15361 agattgtttt cgacgatgta acgacgagca tcggcgaggg tgttgatcat atgatcctga
    15421 tagcggttta acggttcgag atacaccacg gttccggtac gcgcggcgac ctgttccagt
    15481 acgcgcaggg aatcactcac cagtttgcgg tcgccgtcca ggctacgcgg cgaggtcatc
    15541 ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cccccccact tctgccagcg
    15601 cctcgagaat gcgttcgatc tgctttaagc cattaagacg acgctcttcg ataaagtcac
    15661 caatccagcc gtcatagccg ccgcaggcgg tggtcactgg taaaccggtt tctttgatcg
    15721 ccgctttgac ttcttcgatg ttgttgacca gcagtttgcc gtcaatctca aaaccatcga
    15781 agcccatctc tttgatataa cgaaatctct cgcggatatt ttccggaaag aatgcctgat
    15841 tctgtgtgcc gattttcatt ttttcattcc tttgaattaa aacgtaacgc ccattttgat
    15901 gctctgttcc ggatgctgat cgacatactg catgtagctt tccgggctgt tggcaaaggt
    15961 cactaccggg tcgattaaat cttcgcaatt gagataaccg ttcatcagca gttcccagca
    16021 ggtttcttca atacgcttac ggctccagcg cggataatcc gggttcggtt cgctgcacgc
    16081 gcgggagaaa acaattttgg cgttattgaa atgcgcttcg cgtccgaggt taaaaccttc
    16141 agcaaatggt ttggcaaacg cgacatagga gatggtgccg ccgtaggcca ggccgcgcag
    16201 cgccgattgc agcgcgtcgg cgtaaccgct ggtttcgata atcacatcag ccccctgctt
    16261 gccggtcagc gttttgatct ctttgcctac gtcagtgcca atggggttaa ggcagaaatc
    16321 agcaccgtgg cgacgggcaa tatcacagcg atgggcgata ggatcgacgc caattaccac
    16381 cgatgcgcca gcgcgtttcg ccagttggat ggcaatttga ccgatcgcgc caagccctac
    16441 caccacgaca aaatccccta cgcgtacgtt ggcatcgcgc acgccgctca tggcaaactg
    16501 cgccgggtcg taacagacgg cgtttttcca ggagctgcct tgcggcattt tgcgcagctt
    16561 gtagttattc actgcgttaa tgatgaccgt ctcggagagc gggccgtagc cgcatacgct
    16621 gtcgcccacc gcgtagtctg taacgtcgct gccgcactcg ataatgtcgc caaccaccat
    16681 gttgccaagc tggaatttgc caaactcaat gccgcgcggc gcatctgccg gacgcggggt
    16741 gaacatctgc cactcgccgt taaagtcttc atcgataaac gggctggcgg cgcggaagtc
    16801 gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg cggatcttca cttcattggc
    16861 taaaatcgcc cgatcttcat actcaaccag cgcagcaaca cgcggtgctg tggctactaa
    16921 ctttttcatg taaaactcct tgtaaagaaa ctggccgctt tagcccttca cgccaccggc
    16981 ggtcaaacca ctcttaataa aacgttccaa aagcgcatac ataatcacca ccggcaatgc
    17041 ggtcaccagc gaggccgcca tcatccgtcc ccagatgtaa tcaggcgtac tgaacagcgc
    17101 gttcaggccc actggtaagg tgaaattgct ggcgctggag aggaaaatcg acgcaaacag
    17161 atagtcgttc cacgccacca taaagcagta gacgaacacc gatatcagcc cggacatcgc
    17221 caggggcacg gtgatacgga agatgatttg cagacggttg aggccatcca tcatcgccgc
    17281 ttcttcgatc tcatcaggga tggtgtcgaa gtaacttttc agcataaaca cggcggtggg
    17341 cagggtttgc gtcaccatgg taatgatcag cgccatctcg gtgtcgtaaa taccaagcgc
    17401 ggtgatgatt ttgaacagag gcaccaccag caaaatgcca gagaacatat acaccgtgta
    17461 aaagctggcg ttgatggtca tccggccttt aaagcgcagg cgggaaagcg cgtaagctcc
    17521 gagaatgccg agaaataccg ccaccacgga ggaaaccacc gacaccacca tgctgttacg
    17581 gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg tcgacgtaat gctccagcgt
    17641 ccactgttgc ggcagcagcg taggatgcag cgatatcgcc tctttcgcgc ttttgaacga
    17701 ggtcatcagc atcacaaaaa acggaaacag cgtgatgatg agaaacagcg ccagcccgca
    17761 gtaaaaaccg atacgactga gcgtgcgttt atttgttgcc attgaggttc acccgtttcc
    17821 tggtcagcag aatgacagcg aaaataatga tgaagagcac caccgagatc gccgccgctt
    17881 tccctaagtc attaaaggca aaggcggttt tgtagagata gacaccaaga atatcgacct
    17941 tggttgtcag cagataaaca tcagcgaaca tgtagaacat ccagatggtg cgcagggtca
    18001 ccaccgtcgc caggaccggc ataattgcgg gcagcgtgac gatgcgaaac cgctgccagg
    18061 cattagcgcc atccatctct gccgcttcgt acagcgattt gtcgatggtc tgcaaaatcg
    18121 ccagaaacga gataaaggca tacgggaagt agcgccagat ggcgaacagc accaccagca
    18181 caaagctact gcccggatta tcaaaccaca gcggtgcctg ctcatagaga tgcagaagat
    18241 cgacgccgag gtagttcaca atgccgtagc cgttgttgaa catgtatttc caggcaaaca
    18301 ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag cgaacgcgcg gttttgcgca
    18361 ggcggaactc gcggttgaaa aacatcgcca cggccagccc gagcacggtg ctgcccgcca
    18421 ccaccagcgc cgtataccag accgtcatcc acagcgagtg ccagaagccg ggatcggaga
    18481 gaatgcgcac atagttactc accccaacaa acgttgactc gatgttggga ttgagcggca
    18541 gacgtaaaaa actgatttcg atattcgaca ccatcggcca cgccaccaga ccgcccagca
    18601 gtaataagct gggcgcgaga agcagcagcg caaacggcat atcggaacga cctgaaaaaa
    18661 gcctgttcat tgcggattcc tgtcctgggt ttagtgctgt tcgatcagtt catccagctt
    18721 tttctggctc gcttgcagcg tgccagggag atcggcttta cccacggtga cgttatgcac
    18781 catggagctc accacaccag aacccgtcac atcgcccatg cgggtaaagt ttttatcccc
    18841 tactgcgcca aagacctgaa tatttggcag ttcaccgatt agctgattcg gtagttcacc
    18901 cagcgcctta ataacgtcgt tgtctttcca ggtggcggta gtgacaaccg ctttattcac
    18961 cggcagcgca gcacctggcg acatcatcac ccagtcggca atgttgtccg cctgctccat
    19021 aaaggtgaca aatttttctg ctgcttcggt cttttgtccg gcggtaatgg tcagcgaggt
    19081 caacatgccg tagaccgcag agtttttctc ggttggcacc acgaaaccga cgtttttcgg
    19141 gtcgccttct ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt
    19201 gccgttcata aaggcgtctt tcacttccat gatgtcgtta gaacccggca tggtgttggc
    19261 agcaaggtcg cggtaatagg tcagcgcctg catcatctct ggtgtatcaa gggtgatttt
    19321 gccttcggcg ttaaagacgt tggcctagtt ggataacgca aactgggaga aggattgttc
    19381 cgtcagcacg ctttctgccg taggcagcgc aataccgtat tttttattcg ccgggtcatt
    19441 cagtttctgt gcaacgtcca gcagctgttg ccagtttttc ggctcctcca gccccgcttt
    19501 tgccagcaca tctttgcgat accagatgcc gccaatccag gcgctgacag gaacaccggt
    19561 ccatgcgcta ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc
    19621 accaacgttg ctgatgaccg tggcaaccgc tttgcgatcg ataagctgct ctttatccat
    19681 aactttggcg tagtcatggc tggtttcgat cacttccggc agcgagccgc tacgtgaaag
    19741 ggtgatgact ttggtgttat gagcgtcttc ttccacgggc acttgtttaa cggtgatacc
    19801 tgggttttct ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc
    19861 caccgaagaa tgcataaatt cgatggatac atgggtttta ttttcttctt tacagcctga
    19921 aatcagggcg caggaaacca gtgctgataa cagcacgatt tt
//
LOCUS       DAJTYL010000075        19892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYL010000075 DAJTYL010000000
VERSION     DAJTYL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.075"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(83..289)
                     /locus_tag="OO572_002601"
     CDS             complement(83..289)
                     /locus_tag="OO572_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6661560.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            602..691
                     /gene="kdpF"
                     /locus_tag="OO572_002602"
     CDS             602..691
                     /gene="kdpF"
                     /locus_tag="OO572_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6661561.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            691..2364
                     /gene="kdpA"
                     /locus_tag="OO572_002603"
     CDS             691..2364
                     /gene="kdpA"
                     /locus_tag="OO572_002603"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6661562.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2387..4434
                     /gene="kdpB"
                     /locus_tag="OO572_002604"
                     /pseudo
     CDS             2387..4434
                     /gene="kdpB"
                     /locus_tag="OO572_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4443..5015
                     /gene="kdpC"
                     /locus_tag="OO572_002605"
     CDS             4443..5015
                     /gene="kdpC"
                     /locus_tag="OO572_002605"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6661563.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5008..7688
                     /gene="kdpD"
                     /locus_tag="OO572_002606"
                     /pseudo
     CDS             5008..7688
                     /gene="kdpD"
                     /locus_tag="OO572_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7685..8370
                     /gene="kdpE"
                     /locus_tag="OO572_002607"
                     /pseudo
     CDS             7685..8370
                     /gene="kdpE"
                     /locus_tag="OO572_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8645..8749
                     /locus_tag="OO572_002608"
     CDS             8645..8749
                     /locus_tag="OO572_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6661564.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9060..11257
                     /gene="speF"
                     /locus_tag="OO572_002609"
                     /pseudo
     CDS             9060..11257
                     /gene="speF"
                     /locus_tag="OO572_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11254..12573
                     /gene="potE"
                     /locus_tag="OO572_002610"
     CDS             11254..12573
                     /gene="potE"
                     /locus_tag="OO572_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6661565.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12629..14269)
                     /gene="pgm"
                     /locus_tag="OO572_002611"
     CDS             complement(12629..14269)
                     /gene="pgm"
                     /locus_tag="OO572_002611"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6661566.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14295..14840)
                     /gene="seqA"
                     /locus_tag="OO572_002612"
     CDS             complement(14295..14840)
                     /gene="seqA"
                     /locus_tag="OO572_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6661567.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15025..15789
                     /gene="ybfF"
                     /locus_tag="OO572_002613"
     CDS             15025..15789
                     /gene="ybfF"
                     /locus_tag="OO572_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6661568.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15929..16222
                     /gene="ybfE"
                     /locus_tag="OO572_002614"
     CDS             15929..16222
                     /gene="ybfE"
                     /locus_tag="OO572_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6661569.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16362..16892
                     /gene="fldA"
                     /locus_tag="OO572_002615"
     CDS             16362..16892
                     /gene="fldA"
                     /locus_tag="OO572_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6661570.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17102..17188
                     /gene="uof"
                     /locus_tag="OO572_002616"
     CDS             17102..17188
                     /gene="uof"
                     /locus_tag="OO572_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6661571.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17181..17627
                     /gene="fur"
                     /locus_tag="OO572_002617"
     CDS             17181..17627
                     /gene="fur"
                     /locus_tag="OO572_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6661572.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17711..18037)
                     /locus_tag="OO572_002618"
     CDS             complement(17711..18037)
                     /locus_tag="OO572_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661573.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18074..19480)
                     /gene="chiP"
                     /locus_tag="OO572_002619"
     CDS             complement(18074..19480)
                     /gene="chiP"
                     /locus_tag="OO572_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6661574.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4586 a         4995 c         5286 g         5025 t
ORIGIN      
        1 catgttaatt atcatggaag acgttgatgt tgaacaccta aaccagcccc ctgatacgag
       61 gctggcttaa gcgcgcaggc aatcagtaaa aatcaccggt tgccttttcg gcctgatcca
      121 tccacaccgg tttatcgctg gttttcgacc agacgcggtg caggtagctg taaaagcggg
      181 cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac gcccgctgca accgcatatg
      241 tacggcgtaa aaagataagc catgcaggat attctctgta gagttccata cttttctccc
      301 ccttttgtat ctacccggtg aatagcaccg gaaaaatgaa tttgtttatc tgatgaaaat
      361 agtaccgcct tttgtgtaat tttactactc atccgaccac ttatttttgc ttattgatgg
      421 tttatttaca ttcacactgt aattaaatta cacaaaagtt aaattaatac taaccatcag
      481 ttaaatcatg gcctttgcca tttttatact ttttttacac cccgtccgca gatttttgcg
      541 aaatctttgc agccagaatt ctactcttcc ggtatcactt ttaggccact ggaggtgcac
      601 tgtgagtgca ggcgtgatag ccggcgtgtt gctggtgttt ttattactgg gctatttggt
      661 ttatgccctg atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgattgcc
      721 acgtttttac tggtattaat ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg
      781 attaatgaca ttcctcttcc aggtacagcg ggcgttgaac gtatactctt tcgtttacct
      841 ggcgtctctg accatgagat gaactggaag caatatcttt gtgccattct cggcctgaac
      901 atgctggggc tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat
      961 ccacagcagt tgccggggct gtcgtgggat ctggcgctga ataccgccgt cagctttgtc
     1021 acgaatacca actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg
     1081 gcaggcttaa cggtacaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc
     1141 ttcatccgtg cgtttactcg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg
     1201 ctacgcatca cgttatgggt gctagtccct gtggcgttgt tgatcgcact gttttttatt
     1261 caacaaggtg cgcagcaaaa ctttctgccc tatcaggcag tgaataccgt tgaaggagcg
     1321 caacagctgt tacccatggg accagtagct tctcaggaag cgatcaagat gctcggtact
     1381 aacggcggtg gcttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg
     1441 accaacttcg tgcagatgct ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt
     1501 ggtgaagtga cgggcgatcg ccgccagggg cgcatgttgc tgtgggcgat gtcagtgatt
     1561 tttgtcatct gcgtaggcgt ggttatgtgg gcagaagttc agggtaatcc tcatctgctg
     1621 gcgctcggcg cagacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg
     1681 gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct gtggcgcggt gattgcgatg
     1741 catgattcgt ttaccgctct cggcggcatg gtgccgatgt ggctaatgca aattggtgaa
     1801 gtggtgttcg gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg
     1861 gtgtttattg ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatagac
     1921 gtacgcgaga tgaaactgac tgcactggcg attctggtta ctccaacact ggtactgatg
     1981 ggcgcggcgt tggcgatgat gaccgacgcc ggacgtagcg ccatgcttaa ccctagcccg
     2041 catggtttta gcgaagtgct gtacgccgtg tcgtccgccg ctaacaacaa cggcagcgcc
     2101 tttgccggat taagcgccaa ctctccgttc tggaactgtt tactggcgtt ctgcatgttt
     2161 gtcggtcgct tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa
     2221 aagagccaac ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg
     2281 ttaatcggca ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt
     2341 ccggtggcgg aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca
     2401 actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt
     2461 aaacccgcag gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct
     2521 gaccacctgt attagcatcg cgatggcaag cggtgcgatg cctggcaatg cgctgtttag
     2581 cgcggcaatt agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct
     2641 ggcagaaggc cgcagtaaag cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt
     2701 tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca
     2761 actgcgtaaa ggtgacatcg tgctggtaga agctggcgat attatcccct gcgatggtga
     2821 agtcattgaa ggcggcgcat cggtcgatga aagcgccatc accggggaat cggcaccggt
     2881 gatccgtgaa tccggcggcg attttgcctc cgttaccggc ggcacgcgta ttctttctga
     2941 ctggctggtg attgagtgta gcgttaaccc cggtgagaca tttctggatc ggatgatcgc
     3001 gatggtggaa ggcgcacagc gacgcaaaac accgaacgag attgccctga ctatcctgct
     3061 gattgctctg accatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg
     3121 gggcggtaat gccgtgagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac
     3181 cactattggt ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc
     3241 gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct
     3301 actggataaa accggcacca tcacactcgg taaccgtcag gcgtcggagt ttatccccgc
     3361 gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga
     3421 aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg
     3481 cgatgtgcag tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg
     3541 gatcaacatc gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatat
     3601 tgaggctaac ggcggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg
     3661 tcaaggagcc acgctgctgg tggtggtgga aggttctcgt gtgctgggcg ttattgcgct
     3721 gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgca aatgggcatt
     3781 aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgcgatagc tgcggaagcg
     3841 ggtgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt gatccgtcag
     3901 tatcaggcgg aaggtcgttt ggtagcgatg accggcgaca gcaccaacga tgctacggcg
     3961 ctggcgcagg cagatgtcgc ggtggcgatg aactccggca cccaggcggc taaagaggcg
     4021 ggcaatatgg tggatctcga ctctaacccg accaagttga tcgaggtggt gcacattggc
     4081 aaacagatgc tgatgacccg tggctcgctg accaccttca gcattgccaa cgatgtggcg
     4141 aaatacttcg ccattattcc ggcggcgttc gcggcaacat atccgcagtt aaatgcgctg
     4201 aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcggtcat tttcaacgcc
     4261 ttgattatcg tctttttgat tcctctggcg ttaaaaggcg tgagttataa accgcttacc
     4321 gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg
     4381 tttattggta tcaaagtcat tgatttactg ctaaccgttt gtagtctggt gtgaggttta
     4441 ccatgagtgg attacgtccg gcattatcga catttctctt tctgttattg attactggcg
     4501 gcgtttaccc gctgctgacc accgcactgg ggcaatggtg gtatccctgg caggccaacg
     4561 gttcgttgat tcgtgaaggt gatacggtgc gcggttcggc attaatcggg caaaatttta
     4621 ccggcaacgg ctattttcat ggtcgcccgt cggcaacagc agaaatgccc tataatccac
     4681 aggcttctgg cgggagcaat ctggcagtca gtaacccgga gctggataaa cagatagccg
     4741 cacgcgttgc cgcattacgg gccgctaacc cggacgccag cacgaacgtt ccggttgaac
     4801 tggtgacggc gtcggcaagc ggactggaca ataacatcac cccgcaagcg gcggcctggc
     4861 agatcccacg catcgcaaaa gcgcgtaatc tcagcgttga acagctcacg cagctgatcg
     4921 caaaatacag ccaacaaccg ctggtgaaat atatcggcca gccggtcgtc aacattgtcg
     4981 aactcaatct ggcactggat aagcttgatg aataacgaac ccttacgtcc cgaccccgac
     5041 cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt cttcagtgcc
     5101 tgtgcaggcg tcgggaagac ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa
     5161 gggctggata ttgtggttgg tgtggtagaa acccatgggc ggaaagatac cgccgccatg
     5221 cttgaagggc tggctgttct gcctctgaaa cgccaggcgt accgtgggcg gcatatcagc
     5281 gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc tgatcttaat ggacgaactg
     5341 gcgcacagta atgcgccagg ttctcgtcat cctaaacgct ggcaggatat cgaagaactg
     5401 ctggaagctg gcattgatgt tttcacaacc gtcaacgttc agcatctgga aagtctgaat
     5461 gatgtggtca gcggcgtcac cggaattcag gtacgggaaa ccgtgcccga tccttttttc
     5521 gatgccgccg acgacgtggt gctggtggac ttgcccccgg acgatctgcg ccagcggctg
     5581 aaagaaggca aagtctatat tgccgggcag gcggagcgcg ccattgaaca ttttttccgc
     5641 aaaggcaatc tgatcgccct gcgcgaactg gcactgcgcc gtactgccga tcgcgttgat
     5701 gagcaaatgc gcgcctggcg ggggcatcct ggcgaagaga aagtctggca cacgcgcgac
     5761 gcgatccttt tatgcatcgg ccataacacc ggcagcgaaa actggtccgc gcggcggcgc
     5821 ggctgacgtc acggctgggg agcgtctggc acgcggtgta tgttgaaacc cctgccctgc
     5881 atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc
     5941 tgggcgcgga gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg
     6001 cccgtgaaca taatctcggc aagattattc tcggtcgccc agcctcgcgc cgctggtggc
     6061 gtcgggaaac atttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg
     6121 tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctcattta
     6181 aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta
     6241 tcaccttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt
     6301 atctgcttgg cgtggtggtg gcgctatttt atggacgctg gccttcggtg gttgccaccg
     6361 tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct
     6421 ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga
     6481 accttactgc tggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac
     6541 ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg
     6601 ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg
     6661 atgacaacgg taaattgcag ccgttaacac atccgcaagg aattacgcca tgggacgatg
     6721 ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc gacacgttac
     6781 ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac gggctggtgg
     6841 tggtggagcc ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga
     6901 cttttacgct gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac
     6961 aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc
     7021 atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc
     7081 tggcaagcga aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga
     7141 acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta
     7201 atttgaagaa agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgccgg
     7261 aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg
     7321 ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg
     7381 cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg
     7441 atgtctggga taacggcccc ggttttccgc caggccagga gcagacgata tttgataagt
     7501 ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc
     7561 gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg
     7621 cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg
     7681 atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac
     7741 ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct
     7801 ggaagcggca acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga
     7861 tgggattgag tttatccgcg acctgcgcca gtggagcccg gtgccggtga ttgtgctttc
     7921 cgcacgcagc gaagagagcg acaagatcgc cgcgctggat gccggagcgg atgattatct
     7981 gagtaagccg tttggtattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca
     8041 ctccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccgg tgttaccgtc
     8101 gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac accaattgag
     8161 ttccgcctgc tggcggtgct gctcaacaat gccggaaaag tactcaccca gcgccagctc
     8221 cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc actatttgcg tatttatatg
     8281 ggacatctgc gacaaaaact ggaacaggat ccggcccggc ctcgccactt cattactgaa
     8341 accggtattg ggtatcggtt tatgctttga attgttattc aataaattcg cttaaatagt
     8401 gattaattta ttattaaacc tgcgccgaat attattattt ggcaggttta atttcttaac
     8461 gcaaatataa aacacgaaat tacaacattt aagtaacaaa acagcatcta atatggcgat
     8521 attcataatt aatgatttat tatctggggt atgatcgttt tttgttgatc ttcttcacag
     8581 attatagcca tttcatggat agaataactc taccttcaac tgacacagca agcggtaaag
     8641 gtaaatggaa aataataacc gcttaatgcc tcatataagg cggacaaccc atatcatgaa
     8701 gtttacccat cgtaatagtt ttgactttca tttctttaat gcccgctagt cttctgacta
     8761 aagggcaccc caacgtacag gtctccctga ctttaagcat tacaggttaa tacctgtatt
     8821 cctcggtgct catatactgc taaccctttt aaactctaaa taattcgagt cgcagcactt
     8881 gcaacttgag gtatgacgag tatagccagt taccgggctg gtctgggtta ttgcatctgc
     8941 aaaaagcaaa ctactgattt atttatcagc ggtggagctt tgcttttttt ccgacgtgat
     9001 cgatttctcc tttgagaaat tgaggacctg ctattacctg aaataaagag atgaataaaa
     9061 tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga ttgtttttcc actcatcgtg
     9121 catgcgtgaa tattgacgaa agtaattata ttgacgttgc cgccattatt ttatcagtca
     9181 atgatgttga acgtggaaaa ctcgataaaa tagacgctac tggctatggc attcctgttt
     9241 ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata tctgccacgt atttctggtg
     9301 tcttcgagca ttgcgaatca cggaaagagt tttatggacg tcagttagaa accgctgcta
     9361 gccattatga aacgcaactg cgcccgccct tcttccgcgc actggtcgat tatgtcaatc
     9421 agggcaatag cgcttttgac tgcccgggtc atcagggagg tgagtttttc cgtcgtcatc
     9481 cggcagggaa tcagttcgtg gagtacttcg gagaaacgct gttccgttcc gacttatgca
     9541 acgcagacgt agcaatgggc gatctgctga ttcacgaagg cgcgccatgc attgcacagc
     9601 aacatgcggc aaaagtgttt aatgccgata aaacatactt cgttctaaat ggcacttcat
     9661 cttctaacaa agtggtttta aacgccctgc taacaccggg tgatctggtg ctgtttgatc
     9721 gcaataacca caaatctaac caccacggag cgttgctaca ggctggtgca acaccggttt
     9781 atctggaaac ggcacgtaac ccgtatggct ttatcggtgg cattgatgcg cactgttttg
     9841 aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc
     9901 gtcctttccg cctcgctgtg attcagttag gcacctacga cggtacgatt tataacgccc
     9961 gccaggtggt ggataaaatt ggtcatctgt gtgactacat tctgtttgac tccgcatggg
    10021 ttggctatga gcaatttatc ccgatgatgg ctgactgttc gccgctgttg ctggatctta
    10081 atgagaacga tccgggtatt ctagttacgc aatccgtaca taaacagcag gctggttttt
    10141 ctcaaacttc acaaatccat aaaaaagaca gccacatcaa agggcaacag cgttatgtac
    10201 cgcacaaacg catgaacaac gcctttatga tgcacgcctc caccagcccg ttctatccgc
    10261 tgtttgccgc actggatatc aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt
    10321 ggatggattg tgtggtaaat ggcattaatg cccgcaaact gatcctcgat aactgtcagc
    10381 atattcgtcc gttcgtacct gaactggtgg atggtaaacc ctggcagtcg tatgaaacag
    10441 cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc aggggaacac tggcattctt
    10501 ttgaaggcta tgcagagaat caatactttg tcgatccttg caaactgttg ctgacaaccc
    10561 caggtattga tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg
    10621 ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac gccatcctct
    10681 tcctgctgac tccggcagaa gatatggcca aacttcagca acttgtgccc tgctggtacg
    10741 cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa
    10801 acagcatgaa gagcgctacg ccggttatac cctgcgtcag ttgtgccagg aaatgcatga
    10861 tttgtatgcc cgccacaacg tgaaacaact gcaaaaagag atgttccgca aagcgcactt
    10921 cccacacgtc agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga
    10981 gctggttcgt ctaccggatg ccgaaggtcg tatcgctgcc gaaggggcgc ttccttatcc
    11041 tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt
    11101 cagcgctctg gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt
    11161 ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga
    11221 tgcgcaaagc accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg
    11281 ggcgtcgttc agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg
    11341 ctgccgacaa agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc
    11401 gtaggctcaa tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca
    11461 ggtggtatgg gcggttatgc cgagtatgct ttcggtaaat ccggtaactt tattgcgaac
    11521 tatacctacg gcgtctcact gctgattgct aacgtcgcga ttgctatttc ggcggttggt
    11581 tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc
    11641 ggggtgctgt ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc
    11701 agtagcatta ccgtgtgggg gggcattatt ccggtggtag gcctgtgcat tatcggctgg
    11761 ttctggttta gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc
    11821 agtgcagtag gttcttccat cgctatgacg ctgtgggctt ttcttggtct ggagtctgcg
    11881 tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc
    11941 ggtacgttag gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg
    12001 cctaatatgg agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttt
    12061 acaccagaag tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg
    12121 cttggctggc agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc
    12181 cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt
    12241 gtgattatcc agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc
    12301 aacgtgctgg ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg
    12361 gcattagtca ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac
    12421 tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa
    12481 gccatgctgt acggttccat cgtgaccttc cttggttgga cactgtatgg tctggtatca
    12541 ccacgctttg aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt
    12601 gcgaccgctc tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct
    12661 caaccgcttc tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga
    12721 tcttatatgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca
    12781 ctttcagacc gccaatagaa gcaccgttgc ccggagcagc agtcaggcgc gcggtgatcg
    12841 ggtcacctgc cagggtgctg gcgctcacca tttccggaga cagcttagac agcgccgctt
    12901 tttgtgcgga agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg
    12961 ccagttcgtt gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca
    13021 gcagacacat aatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga
    13081 aggaagcccc tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa
    13141 caaaccattt gaaacctacc ggtacttcta ccagcttacg gcccaagtca ttgaccacac
    13201 ggtcgatcat cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg
    13261 gacgatgctg gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac
    13321 ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga
    13381 acttatcacg cagtgccagc agacccgcca tcgcacactc ggaggagcag tccatacgga
    13441 tcgcaccgtc tttatcgagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga
    13501 tggtcaggtt gaggttgtaa tactcgccaa tgcgcttcca gtattcgata ccggaaccgc
    13561 ccaacggatc aacgcccagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat
    13621 cggccagccc ttccacgaac ggctgcacca gatcctgctc tttcacatag ccggatgcca
    13681 tcgcttcgtc gagggagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg
    13741 ccttgtcttc caccactttg gtgacgttag tatcagccgg gccaccattt ggcggattgt
    13801 acttgatacc accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca
    13861 gcgggccacc ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggagtga
    13921 agccattgtt ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg
    13981 aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga
    14041 tgccgttttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt
    14101 taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct
    14161 ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa
    14221 tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct
    14281 ttgtctgcaa cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc
    14341 atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc
    14401 acccaatacg gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc
    14461 gtttgttcat ctgccgcaaa gtacacgcgt gtacgaccgt gcaacgattc cgttgcttcg
    14521 gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc
    14581 gctcgctttt gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg
    14641 tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct
    14701 ttcgtcaccg gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg
    14761 tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca
    14821 tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca
    14881 agagtatatg aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg
    14941 gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga
    15001 caacgcgaaa aaaagaactt tgccatgaaa ttaaatatcc gcgcgcaaac tgcacaaaac
    15061 cagcacaata attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt
    15121 ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac
    15181 cacggtcttt caccgagaga gccggtaatg aattacccgg cgatggcgca ggatcttgtt
    15241 gataccctgg atgcactgca gatcgacaaa gcaacattta tcggtcactc catgggcggt
    15301 aaagcggtaa tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc
    15361 gatatcgcac cggtcgacta tcacgtacgc cgtcatgatg agattttcgc agctatcaac
    15421 gcggtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat
    15481 cttaatgaag aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc
    15541 tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg
    15601 gcatgggatc accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag
    15661 taccgtgatg atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg
    15721 ggtcactggg tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc
    15781 aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt
    15841 ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct
    15901 gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt
    15961 agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga
    16021 tgaacagctg cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa
    16081 gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc
    16141 gcgtaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct
    16201 gcgtagtcag ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc
    16261 gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt
    16321 tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc actggcatct
    16381 ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg
    16441 gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt
    16501 acgacattct gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg
    16561 atgacttctt cccgactctc gaagagattg atttcaatgg taaactggtt gcgctgtttg
    16621 gttgtggtga ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg
    16681 acatcattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt
    16741 tcgaagcatc aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag
    16801 accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag
    16861 agttgcatct cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg
    16921 ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg
    16981 tacctataca atgtcccagt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac
    17041 gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat
    17101 aatgatacgc attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt
    17161 agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga
    17221 aagtaacgct tcctcgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg
    17281 tcagtgcgga agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta
    17341 cggtatatcg cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg
    17401 aaggcggtaa atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc
    17461 tcgactgcgg caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa
    17521 ttgccgcaaa acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg
    17581 ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg
    17641 agaaaagcca acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta
    17701 cagctctcac ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt
    17761 tcaccgcctg atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc
    17821 gtacactttc ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca
    17881 cgctctggca ggcttcaatt ttttctggcg aaccttgcgc ggtgttgata caggcgctgt
    17941 atgcctcttt cagacggctg tcttcctgtg gtgcggttga ttgcgcacaa gctaccagcc
    18001 ccgatgccat catggcgatg agaatcagtt ttttcattga atgcggcagg cacaaaacct
    18061 gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct
    18121 catcctggaa gatgttaccg taaccgccgc cccagcttgg gatgtcggag tggttgtcgt
    18181 attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga
    18241 ccgcatccag gctgtaggca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg
    18301 gattgctctg ccaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg
    18361 cgaagcctgg aagattccag tttttcaggt catacatcgc actgaagaag accgcttttt
    18421 cgccgttagc gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg
    18481 aagcgtaggt tggggtcata cgttgcagga agtatccctg ctgactgtca gccttaatcc
    18541 aggtgccttc gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt
    18601 gcagccaggc ggtgccgtca taaagatcgt tgacgctgcg atcgtcaact ttatcgcggg
    18661 taccatagaa ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg
    18721 ctttggcaaa atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga
    18781 agttattttt gaagtcgtat ttcgcaccga tggagtgcag ataatcaact ttggtggttt
    18841 tatcgttctg ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc
    18901 acatgtagga gaaactcaag gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg
    18961 ccccctgata agtacccggc ataaagctcc agtgcggcgc taacagcgtt tgaccggttg
    19021 gctgaatgta acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat
    19081 acaggcttat accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg
    19141 aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa
    19201 acgcagcaat atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg
    19261 cattccaggt ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac
    19321 ggtcgcgttc acgctgccag taatagatac cgccggttaa ggttgaatcg tcgatgaatc
    19381 cttcggcgcg agcctccggc attgccataa agcccgacat tgctgtaaca ccggcaatag
    19441 ccagcgccag cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt
    19501 ataaattgct gcaccaaagg tgaacagcga aaaaaaataa aaattaaaaa agtagcgttg
    19561 agaaaagtct cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct
    19621 ttctgtaacc agaatgtaag aacatgagca accgcacata ttttgatgat ttttgttaca
    19681 aggcttaagt ttgcggttga aaggcttgca gaagaataaa aacgtgaaat tccagcaaga
    19741 tgaaagcggt tacattttat ttaattatgt tttgtgattt taaatttatt ttatggataa
    19801 attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa acgccgcaga gcgcggcgtt
    19861 tgggattgtt tgctatttct tgccggatgc gg
//
LOCUS       DAJTYL010000076        19837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYL010000076 DAJTYL010000000
VERSION     DAJTYL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.076"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            7..372
                     /locus_tag="OO572_002620"
     CDS             7..372
                     /locus_tag="OO572_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6661575.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(373..1161)
                     /gene="bioH"
                     /locus_tag="OO572_002621"
     CDS             complement(373..1161)
                     /gene="bioH"
                     /locus_tag="OO572_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6661576.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1199..1882
                     /gene="gntX"
                     /locus_tag="OO572_002622"
     CDS             1199..1882
                     /gene="gntX"
                     /locus_tag="OO572_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6661577.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            1941..2516
                     /gene="nfuA"
                     /locus_tag="OO572_002623"
     CDS             1941..2516
                     /gene="nfuA"
                     /locus_tag="OO572_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6661578.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            2877..4193
                     /gene="gntT"
                     /locus_tag="OO572_002624"
     CDS             2877..4193
                     /gene="gntT"
                     /locus_tag="OO572_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6661579.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4238..6322)
                     /gene="malQ"
                     /locus_tag="OO572_002625"
     CDS             complement(4238..6322)
                     /gene="malQ"
                     /locus_tag="OO572_002625"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6661580.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6332..8725)
                     /gene="malP"
                     /locus_tag="OO572_002626"
     CDS             complement(6332..8725)
                     /gene="malP"
                     /locus_tag="OO572_002626"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6661581.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9337..12042
                     /gene="malT"
                     /locus_tag="OO572_002627"
                     /pseudo
     CDS             9337..12042
                     /gene="malT"
                     /locus_tag="OO572_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12085..13101)
                     /gene="rtcA"
                     /locus_tag="OO572_002628"
     CDS             complement(12085..13101)
                     /gene="rtcA"
                     /locus_tag="OO572_002628"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6661582.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13105..14331)
                     /gene="rtcB"
                     /locus_tag="OO572_002629"
     CDS             complement(13105..14331)
                     /gene="rtcB"
                     /locus_tag="OO572_002629"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6661583.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14512..16110
                     /gene="rtcR"
                     /locus_tag="OO572_002630"
     CDS             14512..16110
                     /gene="rtcR"
                     /locus_tag="OO572_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6661584.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16092..16850)
                     /locus_tag="OO572_002631"
     CDS             complement(16092..16850)
                     /locus_tag="OO572_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6661585.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(16867..17697)
                     /gene="glpG"
                     /locus_tag="OO572_002632"
     CDS             complement(16867..17697)
                     /gene="glpG"
                     /locus_tag="OO572_002632"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6661586.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17742..18068)
                     /gene="glpE"
                     /locus_tag="OO572_002633"
     CDS             complement(17742..18068)
                     /gene="glpE"
                     /locus_tag="OO572_002633"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6661587.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18258..19763
                     /gene="glpD"
                     /locus_tag="OO572_002634"
     CDS             18258..19763
                     /gene="glpD"
                     /locus_tag="OO572_002634"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6661588.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         4628 a         5489 c         5028 g         4692 t
ORIGIN      
        1 tgaagcatgg taaaggcacg gacgccacat cgtggtgaga tctggtattt taaccctgat
       61 ccggttgccg ggcatgaact tcaggggcca cattattgca ttgtggtaac ggacaaaaaa
      121 ctcaacaatg ttttaaaagt tgctatgtgc tgcccgattt caacaggggc aaatgcagca
      181 cgttccacag gggtgacggt gaacgtcctc ccccgtgata cgcaaaccgg taacctgcat
      241 ggcgttgtac tttgtcacca gctaaaagcc gtcgatctta ttgcccgtgg cgctaaattt
      301 cataccgttg ccgatgaaaa attgattagt gaagttatca gtaaactggt gaatttaatc
      361 gacccacaat aattagctct cacttaccac caacaccctc tgcttcaacg ccaccagcag
      421 gtgacaaaac tcgaccggat gcgaaataaa cggcgcatgg gccgctttgg cgaagatata
      481 tgattcgctg tgaggccaaa gtttatccag catcggcacc actttgcgcg gcaccagacc
      541 gtcgagatag ccatacaatc gcaaaaacgg catggacacg ttttgcagcg gctgacggag
      601 atcgaccgtt ttcaggattt ccagcccgcc attaagcacg tcaacctccg gcatcggtaa
      661 cgccagaacg gttttcttca acgcccgcgc atcctggcgc gccgtttcag tccccatggt
      721 ttgtaacgcc aggaaccgct ccactgtacg ctgaaaatca tcactgagtt gctgctgaaa
      781 tcccgccagc acgtccggtt ttatccccgg ccactcgtca cgagcactaa aacaaggtga
      841 cgacgccacg gtgaccagcg cctgaacacg ctcgggatgg gttaacgcaa tctggcttgc
      901 caccagcccg cccagactcc agcctaacca aatggcttta tcaggtgcct gttgcagcac
      961 ggcttcggcc atatcagcaa gtgacagcgc accaaatccc cggctacgcc cgaagccggg
     1021 caggtcaaca aggtgcagcg taaaatgcga gctaagttcc tcgtcaatgc aacgccacac
     1081 ttcggcattc agtccccatc cgtgcagcag cacaagatga acattcccct gacctttggt
     1141 ctgccaccag atgttattca tccgctattg ttctcttttg acttacaagg atgaacatat
     1201 gctaacagta ccgggattat gctggctatg ccgaatgcca ctggcgttag gtcattgggg
     1261 gatttgttcg gtctgttcac gcgccacccg cacagataaa acgttatgcc cacaatgtgg
     1321 attacccgcc acacactccc atcttccctg cggtcgctgc ctgcaaaaac cgccgccctg
     1381 gcaaagactg gtcacggttg ccgactatgc gccgccgtta agtccgctta tccaccagct
     1441 taaattttcc cggcgcagtg aaatcgccag cgccctgtca cgtctgttac tattggaagt
     1501 cttacacgct cgtcgcacca ccgggttgca attgccggat cgcatcatca gcgttccgtt
     1561 atggcagcgg cgtcactggc gtcggggatt taatcagagc gatttgctgt gtcagccgtt
     1621 atcacgctgg ttgcactgcc aatgggatag cgaagccgtc acacgtacac gggccactgc
     1681 gacccagcat tttctcagtg cccggctgcg caagcgcaac ctgaaaaatg cctttcgtct
     1741 tgaattgccc gtgcaaggtc gccatatggt gattgtggat gatgtcgtta ccaccggaag
     1801 taccgtcgca gagattgcgc agttgctttt acgcaatggt gcggcgactg tccaggtctg
     1861 gtgcctttgt cgaaccttgt agagcctcga tgatgggcgt attataacca actaaaatag
     1921 tcaactatta ggccattact atgatccgta tttccgatgc tgcacaagcg cactttgcca
     1981 aactgctggc aaatcaggaa gaagggacac aaatccgcgt atttgtgatt aaccctggca
     2041 cgcctaacgc tgaatgtggt gtttcttatt gtccgccgga tgccgtggaa gccaccgaca
     2101 ctgccctgaa atttgacctg ctgaccgcgt atgttgatga gttaagcgca ccatacctgg
     2161 aagatgcaga gatcgacttt gttaccgacc agttgggttc tcagttaacg ctgaaagccc
     2221 cgaacgccaa aatgcgtaaa gtggcagacg atgcaccgct gatggagcgc gtggagtata
     2281 tgctgcagtc gcagatcaac ccacagcttg ctggtcacgg tggtcgcgtt tcgctgatgg
     2341 aaatcaccga agacggttac gccattctgc aatttggcgg cggctgtaac ggttgttcca
     2401 tggtcgatgt gacgctgaaa gaagggatcg agaagcagct gctgaacgaa ttcccggagc
     2461 tgaaaggtgt gcgcgatctc accgaacacc agcgcggcga acactcctac tactaagtta
     2521 tcccctcttt tgtagattgc ccgatgcgac gctaaagcgt catttcgggc ctacgttgaa
     2581 catgtgccag acgttggcac agcattgctt tggcaaatcc ctcccttcgt ttccccgcga
     2641 taatatgacc aacctctcat aatttaaatt taccccgctc tggtgattct caaacgccaa
     2701 atgttacccg tatcattcac atgggtacca aacatactcc tgacatctga ctacaataat
     2761 tagttttagt gggtatcagt cgtggtgccg caatatctct gttcccgatt gggataatta
     2821 gagtttgtcg tcagaaaatt gacgttaccc ataacaaatg aaaggccagg taaatcatgc
     2881 cattagtcat tgttgctatc ggtgtaatct tgttgttgct cctgatgatc cgcttcaaaa
     2941 tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt tggattaatg caaggaatgc
     3001 cgctggataa agttattggc tccatcaaag ccggtgtcgg cgggacgctc ggtagccttg
     3061 ccctgatcat gggttttggc gcaatgctgg gcaaaatgct ggcagactgc ggtggcgcac
     3121 aacgtatcgc caccacgctg attgccaaat ttggtaaaaa gcacatccag tgggcggtgg
     3181 tgttaaccgg ttttaccgtt ggttttgccc tgttctatga agtgggcttc gtgctgatgc
     3241 tgccgctggt gtttaccatc gcggcttccg cgaatattcc attgctgtat gttggtgtac
     3301 caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg ggcccgactg
     3361 cgattgccac cattttcaat gccgatatgg gtaaaaccct gctgtacggt actattctgg
     3421 caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa ggtatcgata
     3481 agccaatccc ggaaggtctc tacagcgcga aaaccttcag cgaagaagag atgccgagct
     3541 ttggcgtcag cgtctggacc tctctggtgc cggttgtgct gatggcgatg cgtgcgattg
     3601 ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt agcggagttc ctcggtgacc
     3661 cggtaatggc aacgctgatt gccgtgctga ttgcgatgtt cacctttggt ctgaaccgtg
     3721 gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc attgcgatga
     3781 tgctgttgat catcggtggt ggcggtgcgt tcaagcaggt gctggtagac agcggcgtgg
     3841 acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg atggcctggt
     3901 cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca atcactgcgg
     3961 gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccagagctg atggttattg
     4021 cggtgggttc cggtagtgtg attttctctc atgtgaacga tccgggcttc tggctgttca
     4081 aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg ctggaaacca
     4141 ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt tgataacact
     4201 gactgccgga tgcatcgcat ccgacatcaa ccgcactcta cttcttcttc gctgcagctc
     4261 tgcgccgtct gtccaaatcc ttcagcaact tgttcacgcc atcatcggca aacatcgact
     4321 caagcgttgc ggaaagcttg cgtcgccagt ttttatactg gtaactggtg ccaggaatat
     4381 tcaccggttc ggccatatcc agccagtctt ccggctgtag tcctaacaga gcactgttac
     4441 tgtcggcaat gtagcgctgc aaaccacggt tcagcgtcgg cgtcatcgac atcaacgatg
     4501 ccttatgccc ggcacgtttt ggcagacaac catatttatg cagtgcatcc agcagccctt
     4561 gcttcgccag ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc ggatacagcc
     4621 ccagggtttt gcccagcgtt agatccccgc tctcccagta accacgcagc gttggcaggt
     4681 catgtgtcgc cgcaaccgcc atcgactgct ccggatacgc tttcggtgca cggaacgtct
     4741 tctcgtggtc gttttcgaaa tagagcactt tgtaagagta cacaccgctg ctgcgcagct
     4801 taccaacaat ctctaccggt acggtaccga gatcttcacc aatcaccata cagcgatgac
     4861 gtttactttc gagtgccaga atcgagagca gatcatccac cggatagtga acatacgcgc
     4921 cctgatctgc cgtctcgcca tacggtatcc accacaaacg cagcatcgac atcacatggt
     4981 caattcgtaa tgcgccgcag ttttgcatat tggcacgcaa cagctcgata aacggttcat
     5041 aggcacgcgc ggtgatgata tgcgggtcca ttggcggtaa tccccagttc tgccccaacg
     5101 ggccgaggat atccggcggc gcgccaaccg atgctttcag gcaatatagt tcacggtcac
     5161 accaggtttc cgccccacct tccgctacgc caaccgccag atcacgatac aagccaatcg
     5221 gcatttcata gccctggctt atctcccagc aggcggcaaa ctggctgtaa gccagccact
     5281 gcaaccagag ataaaaatcg acgtcatcac gatgttcttc gcagaactga cgcacttctg
     5341 gtgaatccac gttcttatac atctctggcc atgcaggcca gccccagcgc atttcgtcct
     5401 ctttcacttg ctgggcatgt agcgcatcaa aggcagcctg ccagaacagg ctgtcgccct
     5461 gctctgcaac aaactggcga aacgcggcca tctgctcatc atcacgttgc gcgaaacctt
     5521 tccacgccat tcgtaatgct gtcattttta gggcggtaac cgtggagtaa tcgacccagt
     5581 cggcatcgcg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag
     5641 cctcttcgct aagatggaaa tcttcaacgg cgttaacgtc gatataaatc acattcagcc
     5701 aacggcgaga agacgggctg tatgggctgg cgctctccgg atttgccgga tagagcgcat
     5761 gaatcgggtt caggccaatg aacgacccgc cacgttttgc cacatccacc agcatcgctt
     5821 tgagatcgcc aaaatcccca ataccccagt ttttttccga tcgcagcgta taaagctgaa
     5881 cgcaggcacc ccacagcttt tgtttattca gcaaagcctg cggttcgtaa caacgtttcg
     5941 gggcgacaat cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtt agcgtgtgat
     6001 aaccttccgg cagcttcgtc ggtagattga acgctttgcc ccccgttaca tggcctttgt
     6061 actgcgttcc ttcttcggtg gtcagctgcc agctatattc gccgctgccc tccaccacca
     6121 tcggcatttt tttgccgctg gtataaacca tgacattcgg gactggcgtt accgccactt
     6181 tcgtggcggt acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg gcgctaatcg
     6241 actgcggttt accgtgggca ttgatgtaat tggggctaat ccccgccgcc agcgcggcat
     6301 tatccagacg tttgctttcc atcgagcttc cttagcgttt tgcctgccag atacgagcct
     6361 gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg
     6421 ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcaa
     6481 cataggctgc gaaatccgcc atcaccagat acggatcgcc gccctgtttg ccgatactgt
     6541 gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt tccagctctt
     6601 tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg
     6661 ccagaatagc cttcacttgt tccacggtat gaccaaaaat aaagatattt tcttcaccga
     6721 ctttctcggc gatttcaacg ttcgcaccat ccagcgtacc gacagtaagc gcaccattga
     6781 gcgccagttt catattgccg gtaccggaag cttctttacc tgcagtcgaa atttgttcgg
     6841 agatatccgc cgccgggatc agtttttccg ccgccgaaac gcaataatcc ggcaggaaca
     6901 ccaccttcaa cttatcgcca accagtggat cgttgttgat cacgtcagcc actttgttga
     6961 tcgcaaagat aatattcttc gccaggtagt agcccggtgc cgctttcgcg ccgaagagga
     7021 agacgcgcgg tacgcgatca gcctgcaggt tttcacgaat ttctttgtac aacgccagaa
     7081 tatgcagcag attcaggtgc tggcgtttgt actcatgcag acgtttgatc tgaatatcga
     7141 aaatcgcctg tggattgatc tcaataccgg tacgaacttt cacaaactcc gccagacaga
     7201 ctttattcgc ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca tcagcgaatt
     7261 tttccagatt gatcagctga tcgagatcgt tagcccactc tttttgcagt gatttatcca
     7321 acagagccgc cagtgcaggg ttgcactgtt tgatccagcg acgtggggta ataccgttgg
     7381 taacgttatg gaatttgttc ggccatagct ggtgatattc cgggaacaga tctttcacca
     7441 ccagatccga gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc acacacaggt
     7501 tcgccatatg cacttgttta tcgtgcacca ccgccagttt ggcccacact ttttcatcgc
     7561 ccggccaggt tttctccacc agcgttttaa agcgagtatt aatttcgtta ataatctgca
     7621 tatggcgcgg cagtaagcct ttcaccagtt tcacatccca gcgttccagc gcttctggca
     7681 tcagggtatg gttggtgtaa gcgaaagttt tgctggtgat ggcccaggcg tcatcccagc
     7741 tcatctggtg ctcatcggtc agcacgcgca gcagttctgg aatcgcgata gttggatggg
     7801 tatcgttcag ctgaataact tcgtaatccg ccagttcgtg cagtttacgc cccgccagat
     7861 gatggcgacg caaaatatcc gctaccgaac aggcacactg gaagtattgc tgcatcaggc
     7921 gcagcttttt accggcagta tggttgtcgt ttggatagag aactttggtc agtttttccg
     7981 cattgatgcc ttgctgttcg gcgcgcagga aatcaccgtc gttaaattta gttagatcaa
     8041 acggatgcgc atgcgtcgcc tgccacagac gcagcggctg cgccacgcca ttacgatagc
     8101 cgacaacggg gagatcccac gcttgaccgg taatggtaaa ctccggctcc cagcgtccgt
     8161 ctttcatcac tttaccgcca atccctacct gcacatccag tgcttcgttg tggcggaacc
     8221 acgggtagtt actgcgatgc cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag
     8281 actggcggaa caaaccatat tgatagttca gaccgtaacc cgtcgcagac tgaccgacag
     8341 ttgccattga gtcgaggaag cacgccgcca gacgtcccag accaccgtta cccagcgccg
     8401 ggtcgatctc ttcttccagc aggtcagtca ggttgatgtc ataagccttc aacgaatcct
     8461 gtacatcctg ataccagccg agattcaaca ggttgttgcc cgtcagacga ccaatcaaaa
     8521 actccattga gatgtagtta acatgtcgct gattcgccac cggtttggcg aatggctgag
     8581 cacgcagcat ttcggccagt gcttcactca ctgccagcca ccactggcga ggagtcattt
     8641 cagccgcaga atttaagcca taacgctgcc actgacgtga aagcacttcc tgaaattgct
     8701 tatcgttaaa aataggttgt gacataggag ttccactttt cttagatttt caacacaacg
     8761 ttatcgctag tttgccaggc tcgatgttga ccttcctcat cctgcggggg attaggcagg
     8821 gaggagttgc ggggatgagc aaggaaatgt gatctcgacc acttaaagct agtgcaaacc
     8881 acaggattag cagcaaatca atgccataca gcgcagaaaa tctgtatcta agtgcaaaaa
     8941 atggccgttg cgtattttca aaaagcggaa ggtaactcta taaattaagt aaaggagtga
     9001 aacagtttca caagtaaaat atccagtgtg ctccatctca ttcttaatag atttattaag
     9061 atcatctttt tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat
     9121 ctgatgaaca taagggaaac cagtattcac gctggatcag cgtcgtttta ggtgagttgt
     9181 taataaagat ttggaattgt gacacagtgc aaattcagac acataaaaaa acgtcatcgc
     9241 ttgcattaga aaggtttctg gccgacctta taaccattaa ttacgaagcg caaaaaaaat
     9301 aatatttcct cattttccac agtgaagtga ttaactatgc tgattccgtc aaaactaagt
     9361 cgtccggttc gactcgacca taccgtggtt cgtgagcgcc tgctggctaa actttccggc
     9421 gcgaacaact tccggctggc gctgatcacg agtcctgcgg gctacggaaa gaccaccctc
     9481 atttcccagt gggcggcagg caaaaacgat atcggctggt actcgctgga tgaaggtgat
     9541 aaccagcaag agcgtttcgc cagctatctc attgccgccg tgcagcaggc aaccaacggt
     9601 cactgtgcga tatgtgagac gatggagcaa aaacggcaat atgccagcct gacgtcactc
     9661 ttcgcccagc ttttcattga gctggcggaa tggcatagcc cactttatct ggtcatcgat
     9721 gactatcatc tgatcactaa tccagtgatc cacgagtcaa tgcgcttctt tattcgccat
     9781 caaccagaaa atctcaccct ggtggtgttg tcacgcaacc ttccgcaact gggcattgcc
     9841 aatctgcgtg ttcgtgacca actgctggaa attggcagtc agcaactggc atttacccat
     9901 caggaagcga agcagttttt tgattgccgt ctgtcatcgc cgattgaagc cgcagaaagc
     9961 agtcggattt gcgatgacgt ttccggttgg gcgacggcac tatagctaat cgccctctcc
    10021 gcccggcaga atacccactc agcccataag tcggcacgcc gcctggcggg aatcaatgcc
    10081 agccatcttt cggattatct ggtcgatgag gttttggata acgtcgatct cgcaacgcgc
    10141 cattttctgt tgaaaagcgc cattttgcgc tcaatgaacg atgccctcat cacccgtgtg
    10201 accggcgaag aaaacgggca aatgcgtctc gaagagattg agcgtcaggg gctgttttta
    10261 cagcggatgg atgataccgg cgagtggttc tgctatcacc cgctgtttgg caacttcctg
    10321 cgccagcgct gccagtggga actggcggcg gagctgccgg aaatccaccg tgccgccgca
    10381 gaaagctgga tggcccaggg atttcccagc gaagcgattc atcatgcgct ggcggcaggc
    10441 gatgcgctga tgctgcgcga tattctgctt aatcacgcct ggagtttgtt caaccatagc
    10501 gaactgtcgc tgctggaaga gtcgcttaag gccctgccgt gggacagttt gctggaaaat
    10561 ccgcagttag tgttattgca ggcgtggcta atgcaaagcc aacatcgcta tggcgaagtt
    10621 aacaccctgc tagcccgtgc tgaacatgaa atcaaggaca tcagagaagg caccatgcat
    10681 gcagaattta acgctctgcg cgcccaggtg gcgattaacg atggtaatca ggatgaagcg
    10741 gaacggctgg caaaactggc actggaagag ctgccgccgg gctggttcta tagccgcatt
    10801 gtggcaacct cggtgctggg tgaagtgctg cactgcaaag gcgaattgac ccgctcactg
    10861 gcgctagtgc agcaaaccga acagatggca cgccagcacg acgtctggca ctacgcctta
    10921 tggagtttaa ttcagcaaag tgaaattctg tttgcccaag ggttcctgca aaccgcgtgg
    10981 gaaacgcagg aaaaagcgtt ccagctgatc aacgagcagc atctggaaca gctgccaatg
    11041 catgagtttc tggtgcgcat ccgagcacaa ctgttatggg cctgggcacg gctggatgaa
    11101 gccgaagcgt cggcgcgtag cgggattgaa gtcttgtcgt cttatcagcc acagcaacag
    11161 cttcagtgcc tggcaatgtt gattcaatgc tcgctggccc gtggtgattt agataacgcc
    11221 cgtagccagt tgaaccgtct ggaaaacctg ctggggaatg gcaaatatca cagcgactgg
    11281 atctctaacg ccaacaaagt ccgggtgatt tactggcaaa tgaccggcga taaagccgcc
    11341 gctgccaact ggttgcgtca tacggctaaa ccggagtttg cgaacaacca cttcctgcaa
    11401 ggtcaatggc gcaacattgc ccgcgcacaa atcttgctgg gcgagtttga acctgcggaa
    11461 attgttctcg aagaactcaa tgaaaatgcc cgcagtctgc ggttgatgag cgatctcaac
    11521 cgtaacctgt tgctgcttaa tcaactgtac tggcaggccg gacgtaaaag tgacgcccag
    11581 cgcgtgttgc tggacgcatt aaaactggcg aatcgcaccg gatttatcag tcattttgtc
    11641 atcgaaggcg aagcgatggc gcaacaactg cgccagctga ttcaacttaa tacgctgccg
    11701 gaactggaac agcatcgcgc gcagcgtatt ctgcgagaaa tcaatcaaca tcatcggcat
    11761 aaattcgccc atttcgatga gaatttcgtt gaacgtctgc taaatcatcc ggaagtgcca
    11821 gaactaatcc gcaccagccc gttgacccaa cgtgaatggc aggtcctggg gctgatctac
    11881 tccggttaca gcaatgaaca aattgcgggc gaactggaag tggcggcaac caccatcaag
    11941 acgcatatcc gcaatctgta ccaaaaactc ggcgtcgccc accgtcaggc tgccgtgcag
    12001 cacgcgcaga aactgctgaa gatgatgggt tatggggtgt gagtttagcc ggataatgca
    12061 ttggatccgg tttacatctt tacatcattc aatactcacc cgcgttacgc catctgtttc
    12121 tatcaaacca aaccgcaccg gcaagaaacg ctccaccacc gcgatattgg tcagcagatg
    12181 gcatgaagga tgggcgaccg taaattctcc cgcgcccgcc agcgccatcg gcagtaccag
    12241 ttggtcggcg agatattccc ccaccgccgc tgggcttgcc aggtagcgtt tcacctcttt
    12301 cactaactgc gcggctacca cctcggcact gacgcgcttt tcaccgacga cgaaaaagcg
    12361 ttcagtgata ttttcacttt cgacttccag cgagacggta ttacccggcc cctggtcgcg
    12421 cggcaggtta tgaatattct gttcatgcag ggaaaaaatc cccgccagtg tagcgatttc
    12481 acgctcagca acatggcgcg gcacaccagc taatagaact tctccacgca tctgcacaat
    12541 gttcccgcgc tcgccaagtt gcaaggtgct aaacgatgcc acgggcgaga cttccgttgc
    12601 caccacaccg cctccggcag gataaaaacc gtgacgtaac agcgtggttt gctgatgaat
    12661 tcctattttc gccagcagcg gctccagcac ccggcggata aaatccgcag gcggcgccga
    12721 cgggttatcg gtgccgccgc tcacttcaac acgcgaaggt ccatcggcaa accacagcgc
    12781 gggcagcacc gtttgcagca ccagcgtaca acttccggcg ctaccgatag caaagcggta
    12841 atcgccgccg cgcacggtgc caggccggaa gaccagacgc tgcgatccca gctccgcgcc
    12901 ttccaccgtt gccctacaaa tttccgcagc cgcttttacc gcggtcagat gctggcgcag
    12961 cagccccggt ttcgcccgtc cggctcgaat gccagtgatg gtaaatggct ggccggttat
    13021 catcgacagg ctcagcgccg agcgcaggat ctgcccgccg ccttcgccct gtgcgccatc
    13081 cagcgcaatc atccttttca tcatttatcc ttttacgcac accacctgac gcagggtgta
    13141 gataacttcc accagatcgc tttgtgccgc catcaccgca tcaatatctt tatacgccat
    13201 cgggatttcg tcgatcactt cggcatcttt acggcattcc acatgcgcgg tggcacgaat
    13261 ttggtcttcc acgctgaaca gttttttcgc tttagttcgg ctcattaccc gcccggcacc
    13321 gtggctgcac gaacagaacg actcttcatt tcccagccca cggacgataa agctttttgc
    13381 tcccatcgaa ccgggaataa ttccatattg accagcacgc gcagacaccg cacctttacg
    13441 cgtcacgtag atctcttcac caaagagctg ttctttttgc acatagttgt ggtgacagtt
    13501 gatctcttcc atcgccagcg tttgtggctg tctgaccgtt ttctgcgtaa tgctctgcaa
    13561 cgccgttacc acattttcca tcatcgcatc gcggttaagg ctggcgaaaa gctgcgccca
    13621 ggccacggct cttaggtaat catcaaagta ttccgtacct tccataaagt acgccagatc
    13681 acgcgacggc aacgtctcaa gttggtcctg catctctttt tgcgccagat cgataaagta
    13741 agtcccgatg gcgttaccaa ttccgcgtga accggagtgc agcataatcc acacctgctc
    13801 cgactcatca aggcagattt caataaagtg gttacccgtt cccagcgttc ccaggtgttt
    13861 atagttgtta gtgttaagga aacgcggata tttttgcgtt aaccactgat aaccggcctc
    13921 cagctcagcc catttcgcat cgacgttaaa aggcgggttt tcccatgcgc ctttatcacg
    13981 tttacaacgc ccggtagtac gcccgtgtgg tacggccgtt tcaatcgctt gacgcagctc
    14041 cgccaggttt tcaggcaggt ctgccgccgt taacgcggta cgcagcgcgt tcattccaca
    14101 gccaatatcc acgcccaccg ccgccggaat aatcgcccct ttggtcggga tcacgctacc
    14161 aatggtggaa cctttaccca ggtgtacatc aggcattacc gcaatatgtt tgaaaataaa
    14221 cggcatcttc gccgtattaa taagttgctg acgcgcatcg gcttctaccg gcacgccttt
    14281 ggtccacatt tttaccgggg cattttcagt ggtcagtaat tcgtaattca ttttgttttt
    14341 ctcttttcgt tgtttgcggt cctgataatt gcaaccgtcg tgccagaaaa ttaaataagc
    14401 agattaattt attgtttcta aaaagattat atctttatat ccgtaaacgg agatttcccg
    14461 caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa gatgcgtaaa
    14521 acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg cagtcagcgc
    14581 tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt catcgatcga
    14641 ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa acgcgatatc
    14701 gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca taacccgtgg
    14761 gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga gtttcagcca
    14821 gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca gatttgctgg
    14881 tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc aactccgcgc
    14941 aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga tttaagccgt
    15001 tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga tttcctcaaa
    15061 tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat cgaaaaagtg
    15121 gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg taagtcattt
    15181 ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg cgcgtttgtg
    15241 gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt tggtcatgtg
    15301 aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag cgccaacggc
    15361 ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc aatgctgctg
    15421 aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt gagcagtgat
    15481 tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga aggcaaattc
    15541 cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg tctacgccag
    15601 cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc cacactcact
    15661 ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt tgcaacatct
    15721 ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac gcggatggcc
    15781 acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat aaaccgtctg
    15841 cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg cgcagaggca
    15901 gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat ctgccgccag
    15961 gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca gggcaaagcc
    16021 agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct gacgtgggaa
    16081 gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac ctgcatcacg
    16141 cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct ccccatattg
    16201 accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg gcgcgagttc
    16261 tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag cgagccgtcg
    16321 ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga gataaaatcg
    16381 agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc ggcgagaata
    16441 atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt ggtgacaatg
    16501 cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg
    16561 ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat gcgctctttt
    16621 tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact ggaaggcagc
    16681 gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt gaggtcgcgg
    16741 cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact gacataaccc
    16801 tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat ttataaatcc
    16861 ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg ctaaacccac
    16921 ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat caaaccatcc
    16981 ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa tgccactttg
    17041 cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca cgccagaaag
    17101 cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca gggcgctgat
    17161 aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc cgccgagata
    17221 ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta acgcgtgggt
    17281 gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca gccataacat
    17341 cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg cgatcatcat
    17401 cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat aacggcgata
    17461 atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac gcggatctgc
    17521 cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg actcatccgc
    17581 cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct gcgtcgccat
    17641 gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt
    17701 tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc gtacgccacc
    17761 tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata gaccacatcg
    17821 tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg ataacacatc
    17881 accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt gtcgttggtt
    17941 aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg aatatcgacc
    18001 agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat acattcgaac
    18061 tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca cagtttacgt
    18121 cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt ttttcaatgt
    18181 tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga gctttaacga
    18241 aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc ggcatcaatg
    18301 gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg ctggaggcgc
    18361 aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt ggcctgcgct
    18421 accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc
    18481 tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat cgtccgcatc
    18541 tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg ggtaaacgca
    18601 ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa
    18661 ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg gtactcgcca
    18721 acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc gccacctctg
    18781 ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat
    18841 atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa cagttcttcg
    18901 acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc agccatattg
    18961 tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa gataaacgca
    19021 ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc gatgtggaat
    19081 acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac ctgctgaaag
    19141 tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg acctactccg
    19201 gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc cgtgattaca
    19261 cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc ggcggtaagc
    19321 tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg tattatcagg
    19381 gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt gaaggcgacc
    19441 gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa tcgctggcac
    19501 gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat gcgggaacgg
    19561 taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg aaatacctgg
    19621 tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca aaacaaggca
    19681 tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa tatacgcagc
    19741 agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt tcaatctggc
    19801 ctgagactga tgacaaacac aaaactgcct gatgcgc
//
LOCUS       DAJTYL010000077        19700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYL010000077 DAJTYL010000000
VERSION     DAJTYL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.077"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="OO572_002635"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="OO572_002635"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6661589.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1541..2347
                     /gene="tcdA"
                     /locus_tag="OO572_002636"
     CDS             1541..2347
                     /gene="tcdA"
                     /locus_tag="OO572_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6661590.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="OO572_002637"
     CDS             complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="OO572_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6661591.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="OO572_002638"
     CDS             complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="OO572_002638"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6661592.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4238..4465
                     /locus_tag="OO572_002639"
     CDS             4238..4465
                     /locus_tag="OO572_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6661593.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4816..5733
                     /gene="gcvA"
                     /locus_tag="OO572_002640"
     CDS             4816..5733
                     /gene="gcvA"
                     /locus_tag="OO572_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6661594.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5752..6147
                     /locus_tag="OO572_002641"
     CDS             5752..6147
                     /locus_tag="OO572_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6661595.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6140..7240
                     /gene="rlmM"
                     /locus_tag="OO572_002642"
     CDS             6140..7240
                     /gene="rlmM"
                     /locus_tag="OO572_002642"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6661596.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="OO572_002643"
     CDS             complement(7284..8015)
                     /gene="fucR"
                     /locus_tag="OO572_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6661597.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="OO572_002644"
     CDS             complement(8073..8495)
                     /gene="fucU"
                     /locus_tag="OO572_002644"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6661598.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="OO572_002645"
                     /pseudo
     CDS             complement(8497..9916)
                     /gene="fucK"
                     /locus_tag="OO572_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="OO572_002646"
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="OO572_002646"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6661599.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="OO572_002647"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="OO572_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6661600.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="OO572_002648"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="OO572_002648"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6661601.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="OO572_002649"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="OO572_002649"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6661602.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16327)
                     /gene="xni"
                     /locus_tag="OO572_002650"
     CDS             complement(15572..16327)
                     /gene="xni"
                     /locus_tag="OO572_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6661603.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="OO572_002651"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="OO572_002651"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6661604.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /gene="sdaC"
                     /locus_tag="OO572_002652"
     CDS             complement(17863..19152)
                     /gene="sdaC"
                     /locus_tag="OO572_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6661605.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4865 a         4820 c         5200 g         4815 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgccg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag gggaggggta acctcacatc ctgattattg actaagtgcg
     1381 cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca
     1441 aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta
     1501 gggtgaggga taacttcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1561 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1621 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1681 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1741 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1801 agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1861 tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga
     1921 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1981 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     2041 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     2101 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac
     2161 tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga
     2221 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2281 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2341 gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta
     2401 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga
     2461 ggcgctaagc tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2521 taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2581 aatgcgccct tcgctgttgc caaagaagtg cattttgccg ttttcagcca cggtatatcc
     2641 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2701 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2761 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2821 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2881 accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg
     2941 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     3001 ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     3061 agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct
     3121 tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3181 agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc
     3241 agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg
     3301 ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3361 atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc
     3421 tgaacatccg cggggaaatg caccgtcccc tgagcaccat caaccatcac caccatcccg
     3481 gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt cacgttcgac
     3541 atctgaccca acgccagaat ccgactacgg ggagtaatca gctctggcaa caaatcgaca
     3601 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg
     3661 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3721 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3781 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3841 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3901 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3961 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     4021 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     4081 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4141 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4201 actattaaga tttattaatc gttttaagga gaagatgatg aaaaagactg ccgcaattat
     4261 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4321 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgatatcgg
     4381 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4441 agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4501 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4561 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca
     4621 caaccgtaag ccaaaagttc accagaatac gcattccgat aaaacttttc gttccggctc
     4681 aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc
     4741 tcagattaaa aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat
     4801 caaagtttaa aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat
     4861 gccgcagcac gccatttaag ttttactcgc gcagcagaag agctttttgt gacccaggcc
     4921 gcagtaagtc atcaaatcaa gtctcttgag gattttttgg ggctaaaact gttccgccgc
     4981 cgtaatcgtt cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag
     5041 atattttcgc aattaaccga agcgacgcgt aaactccagg cccgtagcgc caagggggcg
     5101 ttgacggtca gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc
     5161 tttaattcag cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat
     5221 aagctggcgg atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta
     5281 cgggtggaaa aactgtacgc cgaatattta ttgccggtgt gttcgccgct actgctgaca
     5341 ggcgaaaaac ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg
     5401 tcacgccgtg actggcagac atatacccga cagttggggt ttaatcatat caacgttcag
     5461 caagggccaa tttttagtca tagcgccatg gtgctgcaag cggctattca cgggcaggga
     5521 gtggcgctgg caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc
     5581 ccgtttaatg atgttctggt cagtaaaaat gctttttatc tggtttgtca tgacagccag
     5641 gcagaactgg gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa
     5701 caagaaaaat tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc
     5761 cgttttatgc tgattttcgc cgccattagc ggcttcattt ttgtggcact gggcgctttt
     5821 ggtgcgcatg tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccaaaccggt
     5881 ctcgaatacc aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt
     5941 cgcatcagta tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc
     6001 agcggcagcc tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg
     6061 gttggcggcg tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta
     6121 aagcgcaagg gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa
     6181 agagtgcgcc gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg
     6241 cgtgaaagag aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa
     6301 gttaatccgt gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg
     6361 ggaactcctg cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca
     6421 gggtgtagta gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa
     6481 agagttgctg aaattctgcc gtaagttcac tgttccgcta cgcgctgcct tgcgcgatgc
     6541 tggggtgctg gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc
     6601 accaggatgc tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg
     6661 cattccgcgc ctgaaatttc cggcagaggc gccgagtcgt tccacgctca aactggaaga
     6721 ggcatttcat gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg
     6781 ggcggtggat ttaggcgctt gccctggcgg ctggacctac caactggtga agcgcaacat
     6841 gtgggtttat tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt
     6901 gacgtggctg cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat
     6961 ggtatgcgat atggttgaaa aaccggctaa agttgcggca ttgatggcgc agtggctggt
     7021 taatggctgg tgccgtgaaa ccattttcaa tctcaaactg ccgatgaaaa aacgctacga
     7081 agaagtatca cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc
     7141 tcagattcag gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg
     7201 catctgggct gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg
     7261 tctccggcct gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca
     7321 tcagaaataa tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta
     7381 aatttacttt tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg
     7441 taatcagcgt tgatcgcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa
     7501 gaaaaaataa acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg
     7561 taacagccat atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt
     7621 ttaccgagtt catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc
     7681 aactggcgtg ccagatacca gcaagttgaa ctggcgtcta aggctatcac catcccttct
     7741 tcaatccacg cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc
     7801 ctgatgtgaa agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca
     7861 tgattgcgca gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct
     7921 ttactgacct ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc
     7981 aggtcgacta tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac
     8041 cgcccgcgtc atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga
     8101 atattcccgt acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt
     8161 tcataaaacg caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc
     8221 gcattacggt aacgtcgttc tacttcagga tcgagagtgt caccctctac cgccgccatc
     8281 atcaccagcg gcggggcata actgtccagc tcaaataacg ggataatcgc ctggagcaag
     8341 tcgctcacca acaggccatc agcgcggatc acctgcggtt ccatcgaatg ggcgggaaag
     8401 tgagcatcgg aaaaaataat ttcatctcca tgtcccatct ctgccagcac tttcaatagt
     8461 tcgggagaaa ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg
     8521 ttcagtttgc gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg
     8581 gctgttaaat tcccctacgc cataccaacc gaacagcgca gctcctgcga cggtcgtttc
     8641 ggcatcgtcg agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa
     8701 tgtgttgcga cttcctccac cgactaacaa taattcatag gctttaaagt gcccgatttt
     8761 ttccagcatc tgtagattgc gctgtaattg cgcagttaac ccttccagcg ccgcgcgata
     8821 gaaatgcccc cgcgtggtat taagcgtcac tccttgccag ccagcgttct gacacgacaa
     8881 taaatcacac tgcattttca caccatccgc gccaggtgcg atcagacgag cttcttcaat
     8941 caacatttgc cagggtgttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc
     9001 ggatgccagc cattgcatac ctgggttata caaccatgcc tggctatcta gttcgcaggt
     9061 ggaaccggcg tactgactta acagcgaagt atcaacctag gcgctgcgaa ccattaaaat
     9121 ttcccatgta ccggaggaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc
     9181 gaactgggta tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc
     9241 tgcggcgttg ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag
     9301 tcggcgtgga ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc
     9361 cagcatctgg ctggttccgg ccatcgtgat atcagtagtg aattcgctgg ttaaacggtg
     9421 gttaatcagc gacgaaatga agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt
     9481 ttctttcaac cacaccaact tatataacgt attgaaacta aaggctccga cgccagaaat
     9541 agcctgcaac cgctgtgcgg agattaaccg ttcaatattg tccataaccg ctgctgttcg
     9601 cggacatttc cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc
     9661 atccacacta aaggtggtga cggcgatacc gcggatgtgg cattcagtca gttcactatt
     9721 gatttgccga cagcaatcag caaagcgttg caaaatggca gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggtgtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagggtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggccaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgcagccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct agaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcgcc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgcccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgtgaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg ttgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag tgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ttcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgccaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tgcttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgtgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccggggg
//
LOCUS       DAJTYL010000078        19542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYL010000078 DAJTYL010000000
VERSION     DAJTYL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.078"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(77..1178)
                     /gene="gldA"
                     /locus_tag="OO572_002653"
                     /pseudo
     CDS             complement(77..1178)
                     /gene="gldA"
                     /locus_tag="OO572_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(1189..1851)
                     /gene="fsa"
                     /locus_tag="OO572_002654"
                     /pseudo
     CDS             complement(1189..1851)
                     /gene="fsa"
                     /locus_tag="OO572_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1863..4364)
                     /gene="ptsP"
                     /locus_tag="OO572_002655"
                     /pseudo
     CDS             complement(1863..4364)
                     /gene="ptsP"
                     /locus_tag="OO572_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4673..5752
                     /locus_tag="OO572_002656"
     CDS             4673..5752
                     /locus_tag="OO572_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6661606.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5767..6087
                     /locus_tag="OO572_002657"
     CDS             5767..6087
                     /locus_tag="OO572_002657"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6661607.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6138..8435
                     /locus_tag="OO572_002658"
     CDS             6138..8435
                     /locus_tag="OO572_002658"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6661608.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8401..9279
                     /locus_tag="OO572_002659"
     CDS             8401..9279
                     /locus_tag="OO572_002659"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6661609.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9281..9622
                     /locus_tag="OO572_002660"
     CDS             9281..9622
                     /locus_tag="OO572_002660"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6661610.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9609..10460)
                     /locus_tag="OO572_002661"
     CDS             complement(9609..10460)
                     /locus_tag="OO572_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6661611.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10609..12342)
                     /locus_tag="OO572_002662"
     CDS             complement(10609..12342)
                     /locus_tag="OO572_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6661612.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12525..15176)
                     /gene="ppc"
                     /locus_tag="OO572_002663"
     CDS             complement(12525..15176)
                     /gene="ppc"
                     /locus_tag="OO572_002663"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6661613.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15528..16679)
                     /gene="argE"
                     /locus_tag="OO572_002664"
     CDS             complement(15528..16679)
                     /gene="argE"
                     /locus_tag="OO572_002664"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6661614.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            16833..17837
                     /gene="argC"
                     /locus_tag="OO572_002665"
     CDS             16833..17837
                     /gene="argC"
                     /locus_tag="OO572_002665"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6661615.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17845..18621
                     /gene="argB"
                     /locus_tag="OO572_002666"
     CDS             17845..18621
                     /gene="argB"
                     /locus_tag="OO572_002666"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6661616.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            18682..>19542
                     /gene="argH"
                     /locus_tag="OO572_002667"
     CDS             18682..>19542
                     /gene="argH"
                     /locus_tag="OO572_002667"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6661617.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPD"
BASE COUNT         4432 a         4969 c         5434 g         4707 t
ORIGIN      
        1 gcgtaggcct gataagcgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc
       61 gggagtttgg agtaggttat tcccactctt gcaggaaacg ctgaccgtac tggtcagcta
      121 ccagcagtgc ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tggatggttt
      181 caccttctgc acaagatgct tttgcaacaa tgcgcatttt cgccgggaca tcttctttaa
      241 tatccagttg agcgagagtt attggcaaac ctaccgcatg gctaagtgca gctacggttt
      301 cgatttcctc caccggcgcg ttttccagta ccagctgcgt cagcgtacca aatgccactt
      361 ttcaccgtga taatagtgat gcgcgtccgg gatagcggtc aggccgttat gcaccgcgtg
      421 agccgccgcc agccgccgct ttcaaaacca acgccgctca aataggtgtt cgcttcaatc
      481 acgcgctcca gcgccggggt tactacatgc tgttcggcgg caagcatcgc tttttcgcct
      541 tcttccagca gggtgttgta gcacagttca gccagtgcca gcgccgcctg ggtgcacttg
      601 ccgcccgcca tggtggtcgc gccgctacga gagcaagcac gcgcttcaaa ccaggttgcc
      661 agcgcatcgc cgatacccgc tgctaacaga cgtgcaggtg cgcccgcgac gattttggtg
      721 tcgacaatga ccatattcgg gttatttggc aacagcagat agcggtcaaa ctcaccctca
      781 tcggtgtaga taacagacaa tgcgctgcac ggtgcatcgg tcgaggcgat agtcggtgcg
      841 atcgctaccg gaacacccat gaaatgtgcc agtgctttgg cagtatcgag ggtttttccg
      901 ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg cagacggtcg
      961 atctcatttt gcgaacattc accgccaaac ggcgtaattt ctactaccag tccagcatct
     1021 ttaaagcttt tctcgacagt ggattgagca aaaactaaaa caaatttgtc acccaccact
     1081 aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac gattaatcac atcagcgccc
     1141 tggatgtatt tacccggtga ttgaataatg cggtccataa ttgctccttt agagatgagt
     1201 agtgccaaat gcggcattcc agtcgtgttc gaacttctct atagctgact ctaccgcagg
     1261 ggtgttgagc atttgttgcg ctacatctaa gggcagggtg atggattcac atcctgccag
     1321 taaacagtcc agcgcctgac gcggcgtttt aaagctggct gccagcacca tgctttctgg
     1381 cgcgtgcatt tctaacagcg tttgcagctc ctgaaccgta cgaatgccgt ccccgccctg
     1441 ggcatctacg cggttaacat acggagcaac gtattttgcc cctgccagtg cggctaataa
     1501 cccttctgcg gcgctatata cagcggtgcc aagtgtagta ataccctatt ttttcagtat
     1561 tttaattgct gccagacctt cggaagtcac cgggattttc accacaatac ccggaatagc
     1621 gtcgcgcagg cgcttcgctt cttccaccat cccctgcgcg tcgcggctca tggtctgagc
     1681 aaacagaatg ccctcatcac caatcgcttt ttgcagacgc ggcagcactt cccagatgga
     1741 ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatcg ggaatatgcg
     1801 tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca tgatgtttcc
     1861 ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta
     1921 accagcgcgt tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc
     1981 atgtgctgat cgcccgcagc gtgtttgttc agggttaaca taatgatgaa ttgcgcttca
     2041 tcatcgcccc agcgcaccgg cgcttgcaga cgcgccacgc tgatggtgga ttgctcaatg
     2101 tgttctgatt tgctgtgtgg aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc
     2161 tcgcgcgccc ataagtcggc ttccagttta cgcggatagc ggcagcggcc cgccagcagc
     2221 aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcact ttccagcgtg
     2281 atgcactcgg cggtgaccag cggtgcgtcc tgttgggtca tgcggaattg cgccagcagg
     2341 tgttccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactt gcggcactca
     2401 cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg
     2461 agttcatcca gccctaagcc gaccagcaac ggcagcacgg aacctttcgc tcccagctca
     2521 ccgcacagac caatccattt accctggcga tgcaccgcct gcacgtcgta atcgagcgcc
     2581 cgcaagaatg ccggattcag gctgttgtag tgacgagtaa ccttagcgtt atcgcgatcc
     2641 actgccagca gatattgcgt caggtcatta ctaccaatac taaagaaatc aatctcttcg
     2701 cagcattgat cgatgatgaa catcaccgac ggcacttcca gcataatgcc gagctggatc
     2761 ttctcatcaa acggaatgtg ttcgttacgt agttgctgtt tggcttctgc cagtttttct
     2821 ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt caggctgccg
     2881 tgagcggagg cgcgcaggat cgagcgtagt tgtgtggtaa ataacgacgc gtattcttca
     2941 taaatacgca cggcgcgata accgaggaac gggtttgcct ctgcgggaat gtttagataa
     3001 tcaacgggtt tgtcgccgcc aatgtccata gtgcgcacaa taatgctgcg tccgttggca
     3061 gactccagcg cctgacaaaa aatgttgtac agctcgcttt cgcccggtgc actggtgcga
     3121 tccatataga gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg
     3181 gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat accgtcagcg
     3241 gtacgggctt gttgggtcag ctagacacgc tgttgctcac gcagggcgtc ctgtacgcgg
     3301 gcttcttgct gataataacg agctacggct tctcctggct caaccacaat cgccccggcg
     3361 ttgccgtcga tataaatcgt ttgatgctgc cacggagtga gggcatcaat atccacacca
     3421 accagcgtcg gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt ggtgccgccg
     3481 cttttcagta acaatccttt gaggtgattt ttatccagtt cgaggaactg gctgggggtc
     3541 agttcatcag ccatacaaat ggcggactgc gtcagtttgc ccggtgccgg gaagcgttgc
     3601 tcaccgtaga tttgctggag taactggaag cagacgtcgc gtacgtccag ggcacgttct
     3661 tgcaggtatc tgctgctgga acgggcaaac tcttcgcaaa agtgattcgc actggcgaca
     3721 atcgcttcgg cgcagcttaa tccggcgctg acgcctgcca gtaaatgttc gtgcagggaa
     3781 gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc tggtagcacc gtcgctatcc
     3841 agcagacgaa actcaatgtt tttcagtacc agcgtcaggc cgttttccag tgcggattgc
     3901 tcggcgtcaa cgtctttggc tgcgggaaga ttactcagcg cattgagatc taaagaagag
     3961 atcggcgtca gaatgccgcc tgcactaccg ctgcacacgg tgcgggcgcg gataatttgc
     4021 ggattcagat tggtcagtga aaccggcagt ggttccagtt cgtcagattt aacttccgcc
     4081 agcggcgcat cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg ctggtgccct
     4141 tcctgttcgt cggccccgga aatcagtagc tggcagttat cacccaccag cgtatcggtg
     4201 ccaatcagcg ccagagcgct tttggcgttg cctttacggt cagtacgcag gttatgccac
     4261 tcaatttgag atgaaaaagt attacacagc gtttcaacgt ggcttgccgg acgcgcatgt
     4321 acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctt tctcccggta
     4381 cggttttctg tttacaggat aagagcgcac ggtaacggcc tgccatgtga caaatctgcc
     4441 aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt
     4501 tgcggcatgc ttccggatta tcgcaagtta tgaggcggat cgcatttttg tactgatatt
     4561 acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga ttgcctattg
     4621 tttcctccat caaaaatatg gacatgggct tttgcaagcc cgggagcagc ttatgaatga
     4681 gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt cacacatgat
     4741 tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt atggcaaagg
     4801 cgcagtgccg gatgccgtag ccgatccgaa tttgaaaaaa ctgtttgata tcggcgttgc
     4861 aggtttgacg ctgatggtgc ctttcctcgc cgcttacatt ggttactcca ttgcagagcg
     4921 ttctgcgctg gctccgtgcg ctatcggtgc ctgggttggt aacagctttg gtgcgggctt
     4981 ctttggtgcg ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat
     5041 tccggtgcat aaagttctgc gctcggtgat gcccatcttc attattccta tcatcggcac
     5101 actgattacc gcaggcatca tgatgtgggg cttgggcgag cctgtagggg cgttgaccaa
     5161 cagcctgact cagtggcttc aggggatgca gcagggcagc attgttatgc tggcggtgat
     5221 catggggctg atgctggcgt tcgatatggg cggtccggtt aacaaagtgg cctatgcctt
     5281 catgctgatt tgcgttgctc agggtgttta taccgtggtg gccattgctg ccgtgggtat
     5341 ttgtatcccg ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa atttctccgc
     5401 agaagagcgc gaaaccggca aagcggcact ggtgatgggc tgtgttggcg ttactgaagg
     5461 ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt ccttccatta tggtcggttc
     5521 agtttgtggt gcagtaactg cggcgctggt cggtgcgcag tgctatgcag gctggggtgg
     5581 tctgattgtg ctgccggtgg ttgaaggtaa gttgggttat atcgcagcag tggctgtcgg
     5641 agcagtggtg acggctgttt gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc
     5701 ttcgactgat gaaaaagaag acgacctgga tttggatttt gaaattaatt aattgaggaa
     5761 cgagccatga cgaaaattat tgcagtaacc gcatgtcctt caggtgttgc ccatacctat
     5821 atggcagcag aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa
     5881 acccaggggt caattggtct ggaaaatgaa ctgactgcgg aagacgtggc gagcgctgat
     5941 atggtgattc tgaccaaaga tatcggcatc aagtttgaag agcgttttgc tggcaagacc
     6001 atcgtgcgcg tcaacatcag cgatgcagta aaacgtgctg acgccattat gagcaaaatt
     6061 gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc gggttcgctt
     6121 tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca ctgtttgcca
     6181 atacccgtga aatctcgctg gagcgggcgc tgctttatac cgccagccat cggcaaaccg
     6241 aaggcgaacc ggtgatattg cgccgggcga aagcaacagc gtatatcctt gaacatgttg
     6301 aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa ccgcgcgccg
     6361 ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctggat caattcccga
     6421 cgcgtccgca ggaccgcttt gctatcagcg aagaagataa acgtatctac cgtgaagagt
     6481 tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag atgacagatg
     6541 aagtaaaagc cgcgaccaac acgcagattt tcagcatcaa ccagacggat aaaggccagg
     6601 ggcacattat tattgattac ccacgcctgt tgaatcacgg tctgggtgag ctggtggcac
     6661 agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggcc gcgttgttac
     6721 tgctggaagc ctcgcagaaa cacattttgc gttacgccga actggcggaa acgatggcgg
     6781 caaactgcac agatgcccag cgtcgcgaag agctgctgac tattgcggag atctcccgcc
     6841 ataacgcgca acataagccg cagacgttct ggcaggcgtg ccagttattc tggtacatga
     6901 acatcattct gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc ttcgaccagt
     6961 atatgttgcc gttctatcag gcatctttaa cccagggcga agatccggcg ttcctgaaag
     7021 aactgctcga atctttatgg gtgaaatgca acgacatcgt gctgttgcgc tccaccagta
     7081 gcgcgcgtta tttcgccggt ttcccgaccg gctataccgc actgctcggc gggttaaccg
     7141 agaacggacg tagcgcggtg aacgtgcttt cgttcctttg ccttgacgcc tatcaaagcg
     7201 tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac acgccgttcc
     7261 tgatgaaaac cgccgaaacc attcgcctcg gcaccggtat tccgcaaatc tttaacgatg
     7321 aagtggtggt gccagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt
     7381 ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg catgacatcg
     7441 cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa ggcaatgctg
     7501 cgctgactta tgaaggttta ctggagcaga tccgcgccaa gatcagccac tacatcaccc
     7561 tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggca cctgtaccgc
     7621 tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc gatggcggcg
     7681 cgcgttataa cttctccggc gtacagggaa ttggtatcgc caacctgagc gattctctcc
     7741 atgcgttgaa agggatggtt tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg
     7801 tattaaaagc caactttgca acgccagaag gcgaaaaagt ccgcgctcgc ttaattaacc
     7861 gctttgagaa atacggtaac gatatcgacg aggtggataa cattagcgcc gaactgttgc
     7921 gccactactg caaagaagtg gaaaaatacc agaacccgcg cggcggttac ttcacgccgg
     7981 gatcgtatac cgtttctgct cacgttccgt tgggatcggt ggttggcgcg acgccagacg
     8041 gtcgttttgc cggggaacaa ctggcagacg gcggcttgtc acctatgctg ggtcaggacg
     8101 cacaagggcc aacggcggta ctgaagtcag tcagtaagct cgataatacg ctgctgtcta
     8161 acggtacgtt gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac
     8221 gcaaactggc cgacttctta cgggtgttta cccagcttaa gttgcagcat attcagttta
     8281 acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc
     8341 tggtggtgcg cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg
     8401 atgacatcat ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcgatgat
     8461 gtggcgcgca ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg
     8521 gtggtctttt ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc ggagtcgatc
     8581 tccggcaaaa tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg
     8641 cgtgatgcgg atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt
     8701 gacgctctgg aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggcggc
     8761 gtcacgcttt ctggcggcga agtgttaatg caggcggagt ttgctacccg ttttttacag
     8821 cgactgcggc tgtggggtgt ctcatgcgcc attgaaactg ccggagacgc gccagcaagc
     8881 aagctgttac cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa aattatggac
     8941 gcgactcagg cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt
     9001 ttgctggtga gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc tggtttcacg
     9061 ctcagccggg agaatatgca gcaggcgctg gatgtactga tcccgctgaa aatcaggcag
     9121 atccatctgt taccgtttca tcagtacggc gaaccgaaat accgcctgct ggggaaaaca
     9181 tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat gcgcgaaatg
     9241 gcagaacggg ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt
     9301 aaccgcctgc gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt
     9361 gtgccagtta gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg gaacggagaa
     9421 tcgtttagcg gacgaggata tccgtcgggc ggatgttgct ctgttgatta cggatatcga
     9481 gcttgccggt gccgagcgat ttgaacattg ccgctatgtg caatgcagca tctacgcatt
     9541 cctgcgtgag ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg cgccgcagca
     9601 aacccatctt attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccggcga
     9661 tattccgacg gcgagtgttc ggtgttttta cgaaacagac ggcagaagta gttgctgtcg
     9721 acaaagccgc aggcgtgcgc cacttctttt accttcaggt catagccttt caataacgtc
     9781 ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt
     9841 tgaaacaggt gcgagagata gtttggtgaa atatagaatg cctgtgcgac agattcgcgg
     9901 gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa tagtgcctgg
     9961 ctgcgtgagg cggtctggat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg
    10021 gctacgataa gccttgccgt ttgctgctcc tgcggctgca tttgcatttc attgagcgtt
    10081 tgtagcagaa aagaacctat gcgtgggccg cgtcgggcga cgtgttgctt cgccagattt
    10141 tgatattgtt tgccatccca ttgcacgacg ctgaagccga gttgctgttt gccaaacagc
    10201 acgctaaagg tggtagcggg tgttttccat tgtgggaaat tccagccacc agcggcaacg
    10261 tacagcacat cgccgggaac taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc
    10321 agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgtta
    10381 ctgctggcaa agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga
    10441 tagctgacgt cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg
    10501 cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc
    10561 ggcagtatcg catccggcaa atagcgccag acgcgataca aaggtcatct actgattacc
    10621 cacctgatcg ccatacggca gagtgtcgta atcgatcagc gcgtttttct tgtacgggtt
    10681 accaatccag cgggtagttt ctttgaactg cggattcacc actgaacggg ttggatcgta
    10741 accgtcgtaa gataagcccg ccaaatctga ccaggtgtgg atcagttccg ccaggctgta
    10801 tttacgatcg acatcctgtg agaaatcacg gggatgagtc gcttgccatt tttccgacgt
    10861 ccacagcagg aacggaatgg tgtacatgtg gcgcgtcggg ttgtcttcgt tacgcccctg
    10921 agttttgtgc ggcggcgtgt cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa
    10981 tccgttcggg tctgccgctt taaagtcttt aatcaggctg gcaaccacat gatcgttata
    11041 caggttagcg ttgtcataat cgttatatga ttccagctct tctgcgctta atcctggcgg
    11101 aacatgatcg gtattgccat caaacttgcc ctgattttcc gggtagcggt atttgtattt
    11161 gatatgcgta cccagcagat gaacgatgat cagtttcttc ggcgcagggt cattcagcac
    11221 atcctggaac ggcttcagca cgttggtgtc gtattcacgc gcactctgcg tacgttgctg
    11281 gttcatgtag tactgcttgt cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc
    11341 ggtcatcgtc tgctggttgg tgatccagaa ggttttataa cccgcctgtt tcatcatgtt
    11401 catcagcgac ggctgcgtca gatacagatc cgggttcttt tcattggcaa aggtcagcgc
    11461 ctgttgcagg atttcaatgg tgtacggacg agacgtaact acgttattaa acacggtcag
    11521 attcggatcg gttttatgca gcgcatccag ctccggcgtg gtttcacgcg gataaccgta
    11581 cagactcatg cgtccgcgct gggtcgactc gccaatcacc agcactaaag tgcgcggttc
    11641 gttacccgat tcatctttga aattagccag tggcggcaag gcattatttt cattcagtaa
    11701 ctttgttagc gagtttagtt gctgacgata ctgataatag ccggtcagga attgccacgg
    11761 tgcggcaggc tccatacgcg aggctaggtt atccaacgtt ttctcaaacg gcttgttttt
    11821 gataaacgta ttcatggcga tcggatgcag aatcaagcca taaagcaggg caaaagagac
    11881 aacataacgc cacggctttg gaatatagac cgggcgcagg cgtgtccaca gcagaactgc
    11941 caccgccgta taggccagcg cgataagcac aattttcagg ctgaaatact ggcttaaata
    12001 ctcgctggct tcgttggtgt tggtttcgaa catcacaaac agaacgctct gcgagaactc
    12061 ctgcccgtag atgacgtagt agcacagcgc cgccagagag gccgtccata gcaccacgcc
    12121 gatcactgcg gcaataattt taatccgctt cggaaagagg aataccggga tcaaccacag
    12181 cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca ctataaccac tgatgtaaat
    12241 aatggcctgt agcagagtag agaaaaacca aaagtagagc agtgcccaac ccagggcttt
    12301 ccagctagaa aggggtttag cctggacttc tgtggaatgc atagtaagaa cctgtcttga
    12361 aaaaaatatc gccgaatata acgacaattc cttaaggata tctgaaggta tattcagaat
    12421 ttgaataaaa tgcagacaga aatatattga aaacgagggt gttagaacag aagtatttca
    12481 gaaaaccctc gcgcaaaagc acgagggttt gcagaagagg aagattagcc ggtattacgc
    12541 atacctgccg caatcccggc aatagtgacc attaacgctt gttcgacgcg aggatccggt
    12601 tcctggcctt ctttttctgc ctggcgggag cggtgcagca actcggcctg caatacgttc
    12661 agcgggtcgg tgtaaatatt ccgtagctga atagactctg caatccacgg cagatcggcc
    12721 atcagatggg aatcgttggc aatcgccagc accactttga tgtcttcttc ttgcaggttg
    12781 cgtaactctt tacctaacgg ccacagtgct ttgtctacca gacgttgatc atagtattcc
    12841 gccaaccaca ggtctgcttt ggcgaagacc atctccagca tgccgagacg cgtcgagaag
    12901 aatggccaat cgcggcacat ggcttccagc tcgctctgtt tgccatcttc gaccactttt
    12961 tgcagcgccg tacctgcacc cagccaggcg gggagcatca ggcggttttg cgtccaggca
    13021 aaaatccacg gtatggcgcg cagcgactcg acgccgccgg ttgggcgacg tttcgccgga
    13081 cgtgaaccca acggcagttt gcccaattct tgttccggcg tagcggagcg gaagtaaggc
    13141 acaaaatctt tgttttcacg tacgtagcca cggtagagat cgcaggagat gactgacagt
    13201 tcatccataa tgcgacgcca gctctctttc ggctcaggtg gtggcagcag gttggcttcc
    13261 agaatcgccc cggtgtaaag cgacaggctg ctgacggtga tttctggcag accatattta
    13321 aagcggatca tctcgccctg ttcagtcacg cgcaggccac ctttcaggct tcctggcggt
    13381 tgtgacagca gcgccgcatg ggcaggtgcg ccgccgcgac caatggaacc gccgcgaccg
    13441 tggaacaacg tcagctcaat acccgctttt tcgcaggttt tgattaatgc atcctgtgcc
    13501 tgatattgcg cccaggaagc tgccatcacg cccgcatctt ttgctgagtc ggaatagcca
    13561 atcatcacca tctgtttgcc ctgaatcagg ccgcgatacc agtcgatatt gagcagctgg
    13621 gtcatgacat cgttggcgtt gttcaggtca tcgagggttt caaacagcgg agcaaccggc
    13681 atcgcaaacc cgatacccgc ttctttcagc agcaggtgga cagccagtac gtcggacggc
    13741 gttttcgcca tcgagatcac gtaggcggca atggagcctt gcggcgcttc ggcaatcacc
    13801 tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc
    13861 ggacgtttgg agttcagttc gcggatcagg aacgcctgtt tgtcggcctc tgaccagctt
    13921 tcgtagtcgc cgataccgag gtagcgggtc agctcgccca gcgcttcggt atggcgggtg
    13981 ctctcctgac ggatatcaat acggaccagc ggtacgccga aacatttcac gcggcgcagg
    14041 gtgtcgagca gatcgccgtt ggcgataata cccatgccac acgcctgaag tgactggtag
    14101 caagcgtaga gcggttccca cagttcttcg ttttgtgtca gcagcccttc tggttttggc
    14161 agttcttcgc ctttcaggcg cgcttccagc catgcctgtg tcgccatcag gcgagaacgc
    14221 aggtttttca tcagatagcg atacggttct gcggcacctt cttcgccaac cagcgccagc
    14281 agttcagggg tcgcttcaac catcgacagt tcagaaacca gaacctgaat atctttcagg
    14341 aacagatcgg tggctttcca gcggctgagc agcaggacgt ggcgggtgat atcggcagtg
    14401 acgttcgggt tgccgtcgcg gtcaccgccc atccacgagg taaaacgtac cggaacaaat
    14461 tcgacgggca gtttgtagcc gagattctct tccagttgtt cgttcagttc gcgcaggtaa
    14521 ttcggtacgc cttgccacag gctgttttct actacggcaa agccccattt ggcttcatct
    14581 accgggcttg gacgcagctt acggatttca tcggtatgcc atgactgggc gatcaactgg
    14641 cgcaggcgac gcatcagctg gttgcgttcg tagtcagcga tatctttgtt atcgagctgt
    14701 tttaaacagg cgttcacttc caccattttg tggatcagtg tacgacgggt aatttcggtt
    14761 gggtgagccg tgaggaccag ttccagcgac agcgattcca ctgctttttt gatggtgtct
    14821 tcgctcagtt ccggctggtt tttcagttta cgcagggtgc gggcgatcac ttccgggttg
    14881 ctggcagctt cgcctttcgg cgaaatgctg tggtattgct cggcggtgtt ggccaggttc
    14941 aggaactgac taaacgcacg cgcaacgggc agcagctcgt cgttcgacaa attttgtaag
    15001 gtggtgagca actcctggcg gttagcatca ttgccagcgc gtgaagattt cgacaactta
    15061 cggatagttt ctacgcgttc aagaatgtgt tctcccaacg catccttgat ggtttctccc
    15121 agcactttgc cgagcatact gacattacta cgcaatgcgg aatattgttc gttcatatta
    15181 ccccagacac cccatcttat cgtttgatag ccctgtatcc ttcacgtcgc attggcgcga
    15241 atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca
    15301 aatgctgcga aatcgcttca gcaaacgaat aaatagcagg aatttacgcc attaaattca
    15361 cgacgcttta aataagcgta acttatggaa atgttaaaaa atcgccatac ataacaccaa
    15421 aggtgtaggt cggataagat gcgcaagcat cgcatccgac attattgcgg caccggagtt
    15481 tggcaacggt gccggatgcg gcgcgagcgc cttatccggc ctacgtttta atgccagcaa
    15541 aaatggtgaa ttacctggat aatcaactca cgggtgggtt taataaaccg cgtctccaga
    15601 tattcatcag gctgatgagc ttgattgatg gaacccggac cgagcaccag cgtcgggcat
    15661 aacgtttgaa taaacggcgc ttcggtacag taattcacca cttcggtttt tgctccgagc
    15721 aatttctcaa ccacttcaac cagttgatga ttcggtgggc attcatagcc agggatcggc
    15781 ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt cgctcaccgg agccaatgca
    15841 tcgttgagca aaccattaag ttcattgagt gtcatgccag gctgcggacg aatatccata
    15901 tgcaactcac agcaagcgca aatacggtta gaagcgtcgc caccgtgaat atgcccgagg
    15961 ttgagcgtag ggtatggcac ggtaaatgct tcgtagtgat aacgttcttt caggttatcg
    16021 cgcaattgca aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt aattccgcgt
    16081 gctggatcgc tggagtgccc cgactggccc tgaatacgga tggcgttaga gatatgacct
    16141 ttatgtgcgc gtaccggttg tagtgacgtc ggttcgccaa tgatggcgca atccgggcgc
    16201 agggcggtag tttcggcaaa ataacgcgct ccggccatac tggtttcttc atcagcagtc
    16261 gccagaatgt agagcggttt tttcagtttc gtgacgtcga catcgcgtag cgcatcaagg
    16321 ataaacgcaa aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc
    16381 tccgtcagtg taaacggatc gcgcgtccag cgaccgtcat caaatggcac cgtatcggta
    16441 tgccccgcca gcaacaagcc gccagccccc tgtccgtaac tggccagcat attaaatttg
    16501 ttgcgagttc ctggaacagg ctgcacttcc acattgaagc ccaaatcttt aaaccagtcc
    16561 gccagcagag tgattaaatc tgcattgctt tgatcgagtg cctcttccgt agcgcttatt
    16621 gaaggtgtgg caatcagagc gcggtaaatc tcgataaatg gcggtaattt gtttttcatt
    16681 gttgacacac ctctggtcat gatagtatca atattcatgc agtatttatg aataaaaata
    16741 cactaacgtt gagcgtaata aaacccacca gccgtaaggt gaatgtttta cgtttaacct
    16801 ggcaaccaga cataagaagg tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca
    16861 gcggctacgc tggcgcagag ctagtgacct atgtaaatcg ccatccgcat atgaacataa
    16921 ccgctttgac tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc gatttgcatc
    16981 cgcagctaaa aggcatcgtt gagctgccgt tgcagccgat gtcggatatc agcgagttta
    17041 gcccaggggt ggacgtagtg tttctcgcca ccgcccatga agttagccac gatttagcgc
    17101 cgcagtttct tgaagcgggc tgcgtggtgt tcgacctttc cggcgcgttt cgtgttaacg
    17161 acgccacctt ctatgaaaaa tattacggct ttacccatca atacccggaa ctgctggaac
    17221 aggcagccta cggtctggcg gagtggtgcg gtaataaatt aaaagaagcg aatttgattg
    17281 cggtgccagg ctgttatccg acggcggcac agctggcgct gaaaccgttg attgatgccg
    17341 atcttcttga cctcaatcag tggccggtga tcaacgccac cagcggcgtg agcggtgcag
    17401 gacgtaaggc agcgatttca aatagttttt gtgaagttag cctgcaaccg tatggcgtct
    17461 ttactcatcg ccatcaacca gagatcgcca cacacctcgg tgctgacgtt atcttcaccc
    17521 cacatctggg caatttcccg cgcggcattc tcgaaaccat tacctgccgc ctgaaatcgg
    17581 gtgtgaccca ggcgcaagtc gcgcaagcgt tacagcaggc gtatgcccat aaaccgctgg
    17641 tgcggctgta tgacaaaggc gttccggcgc tgaaaaatgt cgttgggctg ccattttgcg
    17701 atatcgggtt tgccgttcag ggcgagcatc tgattattgt ggcgaccgaa gacaacttac
    17761 tgaaaggcgc ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc tatgcggaaa
    17821 cgcagtctct tatttaaggg tgcaatgatg aatccattaa ttatcaaact gggcggcgta
    17881 ctgctggata gtgaagaggc gctggaacgt ctgtttagcg cattggtgaa ttatcgtgag
    17941 tcacatcagc gtccgctggt gattgtgcac ggcggcggtt gcgtggtgga tgagctgatg
    18001 aaagggctga atctgccggt gaaaaagaaa aacggcctgc gggtgacgcc tgctgatcag
    18061 atagacatta tcaccggagc actggcggga acggcaaata aaaccctgtt ggcatgggcg
    18121 aagaaacatc agattgcggc cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg
    18181 acccagcttg atgaagagtt aggtcatgtt ggactggcgc agccaggttc gcctaagctt
    18241 atcaactcct tgctggagaa cggttatctg ccggtggtca gctccattgg cgtaacagac
    18301 gaagggcaac tgatgaacgt caatgccgac caggcggcaa cggcgctggc ggcaacgctg
    18361 ggcgcggatc tgattttgct ctccgacgtc agcggcattc tcgacggcaa agggcaacgc
    18421 attgccgaaa tgaccgccgc gaaagcagaa caactgattg agcagggcat tattactgac
    18481 ggcatgatag tgaaagtgaa cgcggcgctg gatgcggccc gcacgctggg ccgtccggta
    18541 gatatcgctt cttggcgtta tgcggagcag cttccggcac tgtttaacgg tatgccgatg
    18601 ggtacgcgga ttttagctta agttttgtta gccggaggcg cagctttccg gcattgaatt
    18661 tcaaaataag gaaacagagt tatggcactt tggggcgggc gttttaccca ggcagcagat
    18721 caacggttca aacaattcaa cgactcactg cgctttgatt accgtctggc ggagcaggat
    18781 attgttggct ctgtggcctg gtccaaagcc ttggtaacgg tcggcgtgtt aaccgcagaa
    18841 gagcaggcgc aactggaaga ggcgctgaac gtgttgctgg aagatgttcg cgccaggcca
    18901 caacaaatcc ttgaaagcga cgccgaagat atccatagct gggtggaagg caaactgatc
    18961 gacaaagtgg gccagttagg caaaaagctg cataccgggc gtagccgtaa tgatcaggta
    19021 gcgactgacc tgaaactgtg gtgcaaagat accgttagcg agttactgac ggctaaccgg
    19081 cagctgcaat cggcgctggt ggaaaccgca caaaacaatc aggacgcggt aatgccaggt
    19141 tacactcacc tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt
    19201 gagatgctgg cgcgtgatga aagccgtttg caggatgcgc ttaagcgtct ggatgtcagc
    19261 ccattaggct gtggcgcgct ggcgggaacg gcctatgaaa tcgaccgtga acagttagca
    19321 ggctggctgg gctttgcttc ggcgacccgt aacagtctgg acagcgtttc tgaccgtgac
    19381 catgtgttgg aactgctttc tgctgccgct atcggcatgg tgcatctgtc gcgttttgct
    19441 gaagatctga ttttctttaa caccggcgaa gcggggtttg tggagctttc ggaccgcgtg
    19501 acttccggtt catcattaat gccgcagaag aaaaacccgg at
//
LOCUS       DAJTYL010000079        19252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYL010000079 DAJTYL010000000
VERSION     DAJTYL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.079"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..179)
                     /locus_tag="OO572_002668"
     CDS             complement(<1..179)
                     /locus_tag="OO572_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6661618.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVES"
     gene            complement(210..1076)
                     /gene="ydiB"
                     /locus_tag="OO572_002669"
     CDS             complement(210..1076)
                     /gene="ydiB"
                     /locus_tag="OO572_002669"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6661619.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1088..2360)
                     /locus_tag="OO572_002670"
                     /pseudo
     CDS             complement(1088..2360)
                     /locus_tag="OO572_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2543..3748)
                     /locus_tag="OO572_002671"
     CDS             complement(2543..3748)
                     /locus_tag="OO572_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661620.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(3848..4204)
                     /locus_tag="OO572_002672"
     CDS             complement(3848..4204)
                     /locus_tag="OO572_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6661621.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4340..4447)
                     /gene="rprA"
                     /locus_tag="OO572_002673"
     ncRNA           complement(4340..4447)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO572_002673"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(4633..5745)
                     /gene="ydiK"
                     /locus_tag="OO572_002674"
     CDS             complement(4633..5745)
                     /gene="ydiK"
                     /locus_tag="OO572_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6661622.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6134..9190
                     /locus_tag="OO572_002675"
     CDS             6134..9190
                     /locus_tag="OO572_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6661623.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9187..9597
                     /gene="menI"
                     /locus_tag="OO572_002676"
     CDS             9187..9597
                     /gene="menI"
                     /locus_tag="OO572_002676"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6661624.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            9697..9885
                     /locus_tag="OO572_002677"
     CDS             9697..9885
                     /locus_tag="OO572_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6661625.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10433..10801
                     /gene="sufA"
                     /locus_tag="OO572_002678"
     CDS             10433..10801
                     /gene="sufA"
                     /locus_tag="OO572_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6661626.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            10810..12297
                     /gene="sufB"
                     /locus_tag="OO572_002679"
     CDS             10810..12297
                     /gene="sufB"
                     /locus_tag="OO572_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6661627.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12307..13053
                     /gene="sufC"
                     /locus_tag="OO572_002680"
     CDS             12307..13053
                     /gene="sufC"
                     /locus_tag="OO572_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6661628.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13028..14299
                     /gene="sufD"
                     /locus_tag="OO572_002681"
     CDS             13028..14299
                     /gene="sufD"
                     /locus_tag="OO572_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6661629.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14296..15516
                     /gene="sufS"
                     /locus_tag="OO572_002682"
     CDS             14296..15516
                     /gene="sufS"
                     /locus_tag="OO572_002682"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6661630.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15529..15945
                     /gene="sufE"
                     /locus_tag="OO572_002683"
     CDS             15529..15945
                     /gene="sufE"
                     /locus_tag="OO572_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6661631.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16094..17098
                     /gene="ldtE"
                     /locus_tag="OO572_002684"
     CDS             16094..17098
                     /gene="ldtE"
                     /locus_tag="OO572_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6661632.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17161..17397)
                     /gene="lpp"
                     /locus_tag="OO572_002685"
     CDS             complement(17161..17397)
                     /gene="lpp"
                     /locus_tag="OO572_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6661633.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(17708..19120)
                     /gene="pykF"
                     /locus_tag="OO572_002686"
     CDS             complement(17708..19120)
                     /gene="pykF"
                     /locus_tag="OO572_002686"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6661634.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5044 a         4643 c         4931 g         4634 t
ORIGIN      
        1 gactccacat tggagaggtc ggcaaagtgg tccacacgcc attccagaat atcaaagtcc
       61 gcttcacgat aggcgagagc ttcggatttc acgcgggcga tatctttcgc catcagcgag
      121 acgatgattt taggtgcgcc cgcaccaatg acgagatctt ttacagttac ggttttcatt
      181 ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac ctgtttaaca
      241 tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc ttgccacaac
      301 aacatgccgt atccatcaat cgttttgcaa ccagcttgtt gtgcctgctg caataacttc
      361 gtcatatgcg ggttatacac gcattcagtg accagcagtc ccggatgtaa cagactgata
      421 tcattaacca atgattcatt ctcaaggggt ttcataccta cttttgtgcc attggttaaa
      481 atgtcggcgg aagccagggc ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg
      541 acgacacaat cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac
      601 tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc ctgcgcgcca
      661 atcgccgttg aggcacctcc ggcccctaac agcaccatcg ttttgccttt gatatcaaaa
      721 ccgctctctt taatggcgcg aatatggccc gtgccgtcgg tgttatagcc acgcagatag
      781 ccatcatcat taacgatggt gttgatggca cccaccagtt tggctgctgg cgttaattca
      841 tcaacatatt cacacgccag ttgtttgttc ggcatcgaca caccagttcc gcgcattttg
      901 agggctttta atccttcaat tgctccggga aagctatcgt tatccacttc gaaggccata
      961 taggtaaatg gcaatcccgc tttttctaag gctttattct gcatttcagg cgataaactg
     1021 tggcggatag gataagccat caatccaatc aattcgtatt ttgcggtaac atccataatt
     1081 aactccttta acctctatgc ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa
     1141 accgcacatc attctcagga ataataaaaa cgcggtaata gcggctaaaa acaataattg
     1201 cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac aatgatatat tgcaggccga
     1261 tgttcgacag ataaccggta atcagcggaa taacaaagtt agccagtcca cccatcatca
     1321 tataaatact ggtgactttg gctttgcttt tggggaaaaa ctctgacatg accgaaacac
     1381 cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggcgccg gcattacaca
     1441 ccagtggaga agggtacagg tagataatgg cagctgttat tgttgccagc gcagagttaa
     1501 atacattagc ccagatgggc cggaccattt ttttcagtag tgcggcaaaa ataaagacac
     1561 agaccaacga gcccatactg taataagaga tggtttttaa tgcctcagct tctgacatac
     1621 cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag gtcgagaatg
     1681 cggctacacc gaacagtacc gatgaaacac cttccagcca gactaaaggt tttgctgttc
     1741 atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc
     1801 aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc atagccgtac
     1861 cagatattat tgagcaacat atagctcacc agcattgggt agaacatttg cccaaatgac
     1921 accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca ttccatgagc
     1981 gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac tgccagaccg
     2041 taggcgagag ttaaattcgg gcaagcggga ataccaaaga agaatagcag atacattatt
     2101 actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa
     2161 atactgatca atcgccccaa accgataccg gaaattaaat aggcaatgcc cgcgttgtca
     2221 gtggaaaact tttctgacag agatgtcata ttttgggcaa gcgtaataac actaatgccg
     2281 tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagat
     2341 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     2401 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     2461 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     2521 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     2581 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     2641 tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta atcaacggaa
     2701 tagtaaaggt cgcaatactg cccgtactgt aatagatccc tgtagcttta cctttagcgt
     2761 aagggaaacg ttcagccatt aacgtcaggc caatttgcac aacacctcca gcagaggtaa
     2821 aaccaatgac aaaagcaaat attatcacca cataaaatgt gggatgcagg cagacggtaa
     2881 acagagcaat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat
     2941 tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg tagatactga
     3001 gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc
     3061 actggctaac cagataaaac gttgccattg agatatagcc atataaggta taactggcta
     3121 aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg acaggtaagc
     3181 gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta ataaacataa
     3241 tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact aacaggctaa
     3301 tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa atattggctg
     3361 tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca tcgagaaaac
     3421 tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta ttggtctgca
     3481 ggatgtcaaa aaagaaggcc atatagcagc acatcccgag catgataaaa gggcggcgac
     3541 caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca atgcccagcg
     3601 atgagataac tatcgagaca cccgcggcat tagtctgcca aagtgtctcc agcgaggcca
     3661 tattcaggct catcaaaatg acgcccatac catgcaccag gtaattaaaa tacaacccaa
     3721 gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc tttaaaaagt
     3781 gtagcgatgt tgtgtcagta tccattcatt attgttgtaa caacgacaat tcctttctgt
     3841 ggaaaaatta acaaagccgc tccagatcgt gtgcatataa ttcagcggct accgattgat
     3901 agatcttcca gtcaataaaa ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg
     3961 ggaaaaaacg catggtgttg ttgccgatgc ggttatttat tttctctatg atggcatgaa
     4021 gatgtttttt tcgttgctga cgcatagcca acagttgctc gactacacaa tcaggaatgg
     4081 cgcatttgcc attttcccac tgacgccagc tttcactgtc accggtctgg gcaatccagg
     4141 tcgcgcattc atcaatagtc atggcaaaaa tatggcggag tgcctggagt tcataagcgt
     4201 tcatcgtgta atggctgcat gaaataaaca ataaaatata aataacatta tcagcctgct
     4261 gacggcttga agagagtcac agtatcttgt gcaacattat tggaaagagt gaggggcgag
     4321 gtagcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg gagatgggaa aagactacac
     4381 acagcaattc gttgtttcac tcaggggatt tccatgctta taaatcaata tgttgattta
     4441 taaccgtgag ctaatagtag gcatacggac tttttgcgtc gatcagattc gtctcattag
     4501 tttaaaaact gtaaagaatt tgcgacaggt actaccagct gaatggcggt aagcaggaaa
     4561 atatggggaa gagcaagcaa atatcaatta gcggtgatta acaatcaggc ggcagagccg
     4621 cctgatgaga aattacttat tcggtttctc gatttcgcca agttcttcga gaatttcttc
     4681 cggttggtcc gtcggtggcg ggacttcttc cacccacgcg gcaaacagac gccaggaaac
     4741 ggctaacaga accggaccaa taaacagacc gatcatcccg aaagcaatca aaccaccaat
     4801 aacgccagag aggatcagga tcagcggtaa atcggcaccc atgcgaatta acattgggcg
     4861 gatgacgtta tccagcgtgc caaccacacc gctccacact aacaataccg ttccccaggt
     4921 ggtatcgccg gtccagtaga gccagataat cgccggaatc agtaccggca acgggccaag
     4981 ctggacaagg caggagagga tcattaacac cgttagcaac gttgcataag gtacgccgga
     5041 tacggcgagg ccgataccgc caagcactgc ctgtactaac gccgtcacca ccacacccag
     5101 cgccaccgcg cggatagcct gtgccgccag cagcacggcg gcatctccgc gaacgcctgc
     5161 cagacgggtt gcaaaatggc gaatgccttg tgccacctgt tcaccgcgcc agtacagcag
     5221 ggcactgaag agaagcatca gcgcacaatg caccataaag cgcccgatat gcgccgcctg
     5281 cccaacgaac caggtggtgg tggtgccaat ataagggcgg actttcgcca tgatcgccgt
     5341 accccccata tccagcaagt tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat
     5401 ggtattaagc cacgccagat cgggtaacgt catgtcgccg ctggaaatgg ctttaattag
     5461 cgggccgctg ccgtcgacga tactgttaac cagcaaagca atagggatga taaacaccat
     5521 cactaataac agcgtcatca ccagaacggc gagagagcgg cggccaaaca tgatcttttg
     5581 caaacgtaac aataccggcc aggtggcgat aaccaccgta ccggcccatg caaagccgag
     5641 aataaagggt tgaacaatcc acagacatgc cacaatcatg atggctaaaa acagcaccga
     5701 aagcagaatt tgtgcgacat ccctgggctg acgaacattt accataacta cttttcacct
     5761 tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga aactcacctg cataatgatg
     5821 agcaatttca gcggttttta acaggccgat tctgccctta attctgatgg gcgtgttaga
     5881 aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt
     5941 tgcatgtgct gcggctacgt tcgttcaccc cagtcactta ctattgtaag ctcctggagt
     6001 tcactcgctt gccgccttcc tgcaactcga aatccataga gtaaataaac atcacctttc
     6061 agtgcgctat tgcagccgcc tgaaagaata gtgtatcgac ctacatcaca gaccgcagga
     6121 aagggtcaat ataatgattc cacagatttc cctggcaccc ggcgtcgttc aactggtgct
     6181 taattttttg caagagctgg agcaacaagg ttttaccggc gatgcggcga caagttatgc
     6241 cgatcgtctg acaatgtcga ccgacaacag tatttaccaa cttctccccg atgcggtggt
     6301 atttccgcgt tcaaccgcag atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta
     6361 ttcatcgctg atctttaccc cccgcggcgg cggcaccggc actaacggtc aggcgctcaa
     6421 ccaggggatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga
     6481 agagggctgg gtgcgcgttg aggccggggt gataaaagac caactcaatc agtacctgaa
     6541 accgttcggc tacttttttg cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg
     6601 gatgatcaat accgatgcat ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca
     6661 cgtacttggc gtacgcgcgg tgttgttggg gggcgatatt ctcgatacgc aacctttacc
     6721 cgtcgagctg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa tttataacac
     6781 ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc gacaacttcc ccaaacttaa
     6841 ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga ccgagttcga
     6901 cctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg aagcgcggct
     6961 ggatattacg cgcttgccta aagtgcgccg tctggtgaat gtcaaatatg actcttttga
     7021 ctctgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt
     7081 ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg tcagcgagtt
     7141 gattaccgat atgcctgaca aagagatgct cggactgaac attgtggaat ttgctggtga
     7201 tgatgaggcg ctgattgacg agcgagttaa tgcactctgt gtgcggcttg atgagctgat
     7261 tgccagccaa caggcaggtg tgattggctg gcaggtgtgc cgcgagctgg cgggggttga
     7321 acgtatctat gcgatgcgca aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc
     7381 taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacatctgg ccgattatat
     7441 tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt tcggtcacgt
     7501 cgacgcaggt gtcttgcacg tccgtccggc actggatatg tgcgatccgc aacaagagat
     7561 tttgatgaag caaatctctg atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt
     7621 gtggggcgag cacggcaaag gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga
     7681 actttttgca gaactgcgca aagtgaaagc ggcatttgac ccgcataacc gactcaaccc
     7741 agggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa
     7801 gcgcggtaca ttcgatcggc agatccccat tgcggtacgc cagcagtggc gcggtgcgat
     7861 ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc
     7921 gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga
     7981 atggctgcgc ttgttggcgg atcgcggcgt tgacccactc aaactggaac aagaactgcc
     8041 tgaatccggc gtcagtttgc ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa
     8101 taaaggcgaa tatgacttct cacacgaagt caaagaggcg atgtcgggct gtctggcctg
     8161 taaagcttgt tcgacccagt gccccatcaa aattgatgtg ccggagtttc gctctcgttt
     8221 tctgcagctc tatcacaccc gttatttacg cccgcttcgc gaccacctcg ttgctacggt
     8281 cgagagctac gcaccgctga tggcacgcgc gccgaaaacc tttaacttct tcattaacca
     8341 gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc cactgctgtc
     8401 ggtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcaaacatga cgctggaaca
     8461 gcttgaagct ctcaatgcag agcagaaagc gcgcacggtg ttggtggtgc aggacccctt
     8521 taccagctat tacgatgcgc aagtggtggc ggattttgtc cgtctggttg aaaaattagg
     8581 tttccagcct gtgttactgc cattttcgcc aaatggcaaa gcccagcata tcaaaggctt
     8641 tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa
     8701 gcttgggatg ccaatggtgg gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata
     8761 taaactggcg ctgggcgagg aacgtggcga gtttaacgtc ttacttgcga atgaatggct
     8821 ggcaagcgca cttgagtcac atccagtggc tacagtcagc ggtgaatcat ggtatttctt
     8881 tggtcactgt accgaggtta ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt
     8941 tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt tgctgcggca tggcagggac
     9001 ttacggacat gaagcgaaaa accatgaaaa ttcgctcggg atctatgagt tatcctggca
     9061 tcaggctatg cagcgactgc cgcgtaaccg ctgtctggcg accggatatt cctgccgtag
     9121 ccaggtaaaa cgggttgaag gcacgggggt acgccatcct gtgcaggctt tactggagat
     9181 tattaaatga tatggaaacg gaaaatcacc ctggaagcac tgaatgctat gggtgaagga
     9241 aacatggtgg ggttcctgga tattcgcttt gaacatattg gtgatgacac ccttgaagcg
     9301 acaatgccag tagactcgcg gacaaagcag cctttcgggt tgctgcatgg aggagcatcc
     9361 gtggtactgg ccgaaagtat cggttccgtt gccggttatt tatgtaccga aggtgagcaa
     9421 aaagtggttg gtctggaaat caatgctaat cacgtccgct cggcacgaga agggcgggtg
     9481 cgcggcatat gtaaaccgtt gcatctcggt tcgcgtcacc aggtctggca gattgaaatc
     9541 ttcgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgccat tttgtgacgt
     9601 tgtcaccaga aaagtgtgac gcatattgct tttggtcatt atctgcacag taaacggtgg
     9661 tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact tgatcccacc
     9721 cagctggcca ttgaattttt acgccgtgac cagagcaatc tttcacctgc ccaatatctc
     9781 aaacgtctga aacaactaga acttgaattt gctgacctgc ttactctctc ttctgctgaa
     9841 ttaaaagaag aaatctactt cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc
     9901 gcaagttcgc caaatcgacc tggattaacc tccgtccagg ttcgatgacc ttcttaatac
     9961 tgcttatcta ccgcctgata ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg
    10021 gcgggttagc tttatgagat tattcttatc gccccttcaa gagctaagcc actgagagtg
    10081 ccggagataa gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg
    10141 ttttcttatt tctgaacgct gcactttttt acctatctct taaatagtca ttttcaatac
    10201 aactttttct ttttcatcac agccttaatg ctccttagat aataaccatt atctaacaat
    10261 gagataccta attcttagcg tgcctgttaa cccacacatc aggggctatg cttattaaaa
    10321 gcaggcaaaa agaatgtttt acggtaaagc ccctgcgttt gctgggttga actgataatc
    10381 attatcacta acatgctgtt atacgctgaa agcgatgaag tgaggtaaat agatggacat
    10441 gcattcagga acctttaacc cacaggattt cgcctggcaa ggcttaacgc tgacacccgc
    10501 agcggcgata cacatccgcg agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt
    10561 aggtgtgaag caaacgggct gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc
    10621 ggacaaagac gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc
    10681 gatgccgttt attgatggca cggaagtcga tttcgttcgt gaaggactta atcagatatt
    10741 caaatttcac aaccctaaag cccagaatga atgtggctgt ggcgaaagct ttagggtata
    10801 ggcggtacta tgtctcgtaa tactgaagca actgacgatg tcaaaacctg gaccggcggc
    10861 ccgctgaact ataaagaagg attcttcacc cagttagcca ccgatgagct ggcaaagggg
    10921 ataaacgaag aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga gtggatgctg
    10981 gagtttcgtc taaacgccta tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa
    11041 gcgcactacg acaagctgaa ttatcaggat tacagctact actcagcacc atcgtgcggt
    11101 aattgtgacg acacttgcgc gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc
    11161 tttttaagta aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc
    11221 aaagaggtgg cggtggatgc cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa
    11281 aaactggcgg agcagggaat tattttctgt tcctttggtg aggcgatcca cgatcacccg
    11341 gaactggtgc gtaaatatct cggcaccgtg gtgccgggga atgacaactt ctttgcagca
    11401 cttaatgcgg cggtagcctc tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc
    11461 ccgatggaac tttccaccta ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc
    11521 acaattctgg tggccgacga agacagctac gtcagctaca ttgaaggctg ttccgctccg
    11581 gtgcgtgaca gctatcagtt gcacgcggca gtggtggaag tcatcatcca taaaaacgcc
    11641 gaggtgaaat attccacggt acaaaactgg ttccctggcg ataacaacac cggcggtatt
    11701 ctcaacttcg tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg
    11761 caatcagaaa ccgggtcagc gattacgtgg aaatatccca gctgcatttt gcgcggcgat
    11821 aactccattg gtgagtttta ctcagtggcg ctgaccagcg gtcatcagca agcggatacc
    11881 ggcaccaaga tgatccacat cggtaaaaac accaaatcga ccattatctc gaaagggatc
    11941 tctgccggac atagtcagaa cagttatcgc ggcttagtga aaatcatgcc gacggcaacc
    12001 aatgcgcgca atttcactca gtgcgactca atgctgattg gcgctaattg tggggcgcat
    12061 accttcccgt atgtcgaatg tcgcaataac agcgcacaac tggagcacga ggcaacgaca
    12121 tcacgtattg gtgaagatca actgttttac tgcctgcaac gcgggatcag cgaagaagac
    12181 gccatctcga tgattgttaa cggtttctgc aaagacgtgt tctcagaact gccgctggaa
    12241 tttgccgttg aagcacaaaa actcctcgcc atcagtcttg aacacagcgt cggataagga
    12301 ataaacatgt taagtattaa agatttacac gtcagcgtgg aagataaagc tatcctgcgt
    12361 ggattaagcc tcgacgttcg tcccggcgaa gttcacgcca ttatggggcc aaacggttcg
    12421 ggcaaaagta ccttatcggc aacgcttgcc gggcgcgaag attatgaagt gacgggcggc
    12481 acggttgagt tcaaaggcaa agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa
    12541 ggcatcttta tggccttcca gtatccggtg gagattccag gcgtcagtaa ccagtttttc
    12601 ctgcaaacgg cacttaatgc agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt
    12661 gattttcagg atttgatgga agagaaaatc gctctcctga agatgccgga agatttatta
    12721 acccgttcgg taaacgttgg tttttccggc ggcgagaaaa agcgcaacga tattttgcaa
    12781 atggcggtgc tggaaccgga gttatgcatt cttgatgagt cggactccgg gctggatatt
    12841 gacgcattaa aagtggtcgc cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc
    12901 atcattgtta cgcactacca acgcattctc gactacatca agcctgatta cgttcatgtg
    12961 ctatatcagg gacgaattgt gaaatccggc gatttcacgt tggtcaaaca actggaggag
    13021 cagggttatg gctggcttac cgaacagcag taatgcgctg caacagtggc atcacttgtt
    13081 tgaagctgaa gggacaaaac gctcaccgca agcacagcag catttacaac aattgctgcg
    13141 taccggactg ccgacacgta aacatgaaaa ctggaaatat acgccgctgg aagggctgac
    13201 caatagtcag tttgtcagca ttgcgggaga tatatcccca cagcagcgtg atgccttagc
    13261 gttaacgtta gacgccgtgc ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag
    13321 cgatgcaatt gaaggcagcg gatatgaagt gagcattaac gacgaccgtc aggggttacc
    13381 cgacgctatt caggcggaag tgtttctgca tttgacggaa agcctggcac aaagcgtgac
    13441 gcatatcgcc gtgaagcgcg gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac
    13501 ccagggcgtg gcaggtgaag aggtgaacac tgcccattac cggcatcatc tggatctggc
    13561 ggaaggtgcc gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt
    13621 taccggggca cggttcacta tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct
    13681 ggcgtttgaa aacccggtca gtcaccactt tgcccataac gatttgttgc tggctgacga
    13741 tgccaccgca tttagccata gtttcctgct gggtggcgca gtgttacgac acaacaccag
    13801 tacgcaactc aatggcgaaa acagcacgct gcggatcaat agcctggcga tgccggtgaa
    13861 aaacgaggtg tgtgataccc gtacctggct ggaacacaat aaaggttttt gtaacagccg
    13921 acagttgcac aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa
    13981 cgtcgcgcag cacgccatca aaacggatgg tcagatgacc aacaataatc tgctgatggg
    14041 caaactggcg gaagtggata caaaaccgca gctggaaatc tatgcagatg atgtgaaatg
    14101 cagccacggc gcgacggtgg ggcgtattga tgatgaacag atgttctatc tgcgctcgcg
    14161 cgggatcaat cagcaggatg cccagcagat gatcatttac gcctttgctg ccgaactgac
    14221 ggaagcactg cgtgatgagg ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct
    14281 gccaggaggt gcaagatgac tttttccgtc gacaaagtgc gggccgactt tccggtgctt
    14341 tcgcgtgagg taaacggttt gccgctggct tatctcgaca gcgccgccag tgcacagaaa
    14401 ccgagccagg tgattgacgc cgaggccgag ttttatcgtc atggctacgc ggcggtgcat
    14461 cgcggtattc ataccttaag cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg
    14521 gcatcgctgt ttattaatgc ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg
    14581 gaagggatca atctggtcgc caatagctgg ggcaacagca atgtgcgggc gggcgataac
    14641 atcatcatca gtcagatgga gcaccacgct aacattgtcc cctggcagat gctttgcgca
    14701 cgcgttggcg cagagctgcg tgtgatccct ctcaaccccg acggtacgct gcaactggag
    14761 acgctgccta cgctgtttga tgagaaaact cgcctgctgg caattactca tgtctccaac
    14821 gtgcttggca cagaaaatcc actggcggaa atgatcacgc ttgcgcacca gcatggcgca
    14881 aaagtgctgg tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg
    14941 gattgcgact tttacgtgtt ctccgggcat aaactgtatg gccccaccgg aattggcatt
    15001 ctttatgtga aagaagcctt gttgcaggag atgccgccgt gggaaggggg cggttctatg
    15061 atcgccaccg tcagcctgag tgaaggcact acctggacca aagcaccatg gcggtttgaa
    15121 gccggtacac ccaataccgg gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg
    15181 gcgctggggc ttaatagcat agccgagtat gaacagaatc tgatgcatta tgcgctatca
    15241 cagctggaat ctgtaccgga tctcactctc tatggccaac aaaacaggct tggcgttatt
    15301 gcttttaatc tcggtaaaca ccacgcctat gatgttggca gttttctcga taattacggc
    15361 attgctgtgc gtaccggaca tcactgcgct atgccattaa tggcctatta caacgtccct
    15421 gcgatgtgtc gggcgtcgct ggccatgtat aacacccatg aagaagtgga gcgtctggtg
    15481 accggcctgc aacgtattca ccgtctgctg ggataacagg gaggcactat ggctttattg
    15541 ccggataaag aaaaattgct gcgtaatttt ttacgctgcg ccaactggga agagaaatat
    15601 ctctacatta ttgagctggg ccagcgtctg ccagaattac gcgccgaaga cggaagccca
    15661 caaaatagca ttcagggctg ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag
    15721 ggaattattg aattgcaggg cgacagcgat gcggcgattg tgaaagggct tattgcggtc
    15781 gtctttattc tctacgatca gatgacgccg caggatattg tcaatttcga tgtgcgtccg
    15841 tggtttgaaa aaatggcgct cacccaacat ctcaccccat ctcgttcaca aggtctggaa
    15901 gcgatgattc gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct
    15961 ttcatccggt tggctcacat tgccggatgg tcaatgttta cactgaccgg cacaggagtg
    16021 ttttggtttc aggtgaacat aagcaccatc cgacgtttca gcgtgagtct ccggcaaata
    16081 caggaggttt acaatgaaac gcgcgtcttt gcttacactc acgcttatcg gcgcttttag
    16141 cgccatccag gctgcctggg cggttgatta tccgctacca ccaaccggaa gccgactggt
    16201 tgggcaaaat caaacgtata cggtgcaaga aggggataaa aaccttcagg ctattgcccg
    16261 acgttttgat actgcagcaa tgttgatcct tgaagccaat aacactatcg ccccggtgcc
    16321 aaaacctggt acgacgataa ctattccttc acaactgtta ttacctgatg caccgcgtca
    16381 ggggattatc gttaaccttg cagagctgcg cctttattat tatccgccgg gagaaaatat
    16441 tgtgcaggtt tatccaatag gtattggatt gcaggggctg gaaacgccgg tgatggaaac
    16501 gcgtgttggg cagaaaatcc ctaacccaac ctggacgcct acggcaggca ttcgtcagcg
    16561 ttcgctggag cgtggcatta aattaccgcc agtcgttcct gccggaccaa ataacccgct
    16621 aggacgttac gcactgcgcc tcgcgcatgg taatggcgaa tacctcattc atggcaccag
    16681 tgcgccggac agcgttggtt tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga
    16741 tattaaagcc ttgttctcca gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc
    16801 ggtgaaatat tccgtggaac ctaacgggat gcgttatgtt gaagtacatc gaccactatc
    16861 agcagaagaa cagcaaaacg ttcagacaat gccatacaca ctgccagcag gctttacgca
    16921 atttaaagac aataaggctg tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc
    16981 agggtatccg gtttcggtga gcagtggagc aactcccgca gccagcaatg cgccttcagt
    17041 agagtcagcg cagaatggtg aaccagagca agggaatatg ttacgcgcga cgcagtagtg
    17101 gtaaatagca gacaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc acaggtacta
    17161 ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc
    17221 tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt
    17281 agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc tggagcaacc
    17341 tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag ctttcattat
    17401 taataccctc tagattgagt taatctccat gtagcgttac aagtattaca caaagttttt
    17461 tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg ctcaaagaag
    17521 catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt
    17581 catcttaaat aaagtgagat tatatagtga aatccgattt gattttttat tgcttctggt
    17641 tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt aattcacaaa
    17701 agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt accagtgcac
    17761 cagaaaccat aactacgacg tcacctttgt gtgccagacc gctctgcaga gccagttctt
    17821 tacccagacg gtagaaatca tcagtagaag tgatctcttt aaccagctgc ggcacaacgc
    17881 ctttgctcag taccaactga tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg
    17941 catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgggta gcaaccacga
    18001 tcagcggagc atccagtttt tcagcagttt caacggcacc acggcatacc gcttcggtga
    18061 tgcgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg cggtcggtac
    18121 gttcgcagat ggtcgccatg atagaaaccg cttccagcgg gtatttacct tttgcggatt
    18181 caccagacag catcactgcg tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt
    18241 ctgcgcgagt cgggcgtggg tttttgatca tggaatccag catctgggtc gcagtgataa
    18301 cgactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag ataacttctt
    18361 ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca gaggcttcga
    18421 ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg atgtggatgt
    18481 tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa
    18541 taaaggaagc agcaacaaag tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt
    18601 ctttttcagc cagtgctggc agagcaatgg aaacgccagg caggttcaca cctttgtttt
    18661 cgcccaggtc accgttgttc agcactttac agataacttt gttaccttca atggcggtaa
    18721 cttccatacc gatcagacca tcgtcaacca gtacggtgtt gccaacagac aggtcagtag
    18781 tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat ttatcagtgg
    18841 tgaaagtaaa ggtctgacca gctttcagag aaacatcgtt accgccttcc agtttcatgg
    18901 tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca gttttgctca
    18961 tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca tgagagaagt
    19021 tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct tcagattcgg
    19081 ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta gtctttaagt
    19141 tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta
    19201 cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg ca
//
LOCUS       DAJTYL010000080        18674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYL010000080 DAJTYL010000000
VERSION     DAJTYL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.080"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO572_002687"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO572_002687"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6661635.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO572_002688"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO572_002688"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6661636.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO572_002689"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO572_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6661637.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO572_002690"
     CDS             6038..6658
                     /locus_tag="OO572_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6661638.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO572_002691"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO572_002691"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6661639.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO572_002692"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO572_002692"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6661640.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO572_002693"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO572_002693"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6661641.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO572_002694"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO572_002694"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6661642.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO572_002695"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO572_002695"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO572_002696"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO572_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO572_002697"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO572_002697"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6661643.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO572_002698"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO572_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6661644.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO572_002699"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO572_002699"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6661645.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO572_002700"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO572_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6661646.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO572_002701"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO572_002701"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6661647.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..>18674
                     /locus_tag="OO572_002702"
     CDS             17792..>18674
                     /locus_tag="OO572_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this is the primary sigma
                     factor of bacteria; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6661648.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQ"
BASE COUNT         4410 a         5230 c         4760 g         4274 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagt
//
LOCUS       DAJTYL010000081        18631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYL010000081 DAJTYL010000000
VERSION     DAJTYL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.081"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            650..1339
                     /gene="mutH"
                     /locus_tag="OO572_002703"
     CDS             650..1339
                     /gene="mutH"
                     /locus_tag="OO572_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6661649.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1408..2121
                     /locus_tag="OO572_002704"
     CDS             1408..2121
                     /locus_tag="OO572_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6661650.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2259..2477
                     /gene="ygdR"
                     /locus_tag="OO572_002705"
     CDS             2259..2477
                     /gene="ygdR"
                     /locus_tag="OO572_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6661651.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2585..3625
                     /locus_tag="OO572_002706"
     CDS             2585..3625
                     /locus_tag="OO572_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6661652.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3658..4848)
                     /gene="lplT"
                     /locus_tag="OO572_002707"
     CDS             complement(3658..4848)
                     /gene="lplT"
                     /locus_tag="OO572_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6661653.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(4841..7000)
                     /gene="aas"
                     /locus_tag="OO572_002708"
     CDS             complement(4841..7000)
                     /gene="aas"
                     /locus_tag="OO572_002708"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6661654.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7586..8617
                     /gene="galR"
                     /locus_tag="OO572_002709"
     CDS             7586..8617
                     /gene="galR"
                     /locus_tag="OO572_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6661655.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8624..9886)
                     /gene="lysA"
                     /locus_tag="OO572_002710"
     CDS             complement(8624..9886)
                     /gene="lysA"
                     /locus_tag="OO572_002710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6661656.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            10008..10943
                     /gene="lysR"
                     /locus_tag="OO572_002711"
     CDS             10008..10943
                     /gene="lysR"
                     /locus_tag="OO572_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6661657.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10930..11622)
                     /gene="ygeA"
                     /locus_tag="OO572_002712"
     CDS             complement(10930..11622)
                     /gene="ygeA"
                     /locus_tag="OO572_002712"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6661658.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11751..13169)
                     /gene="araE"
                     /locus_tag="OO572_002713"
     CDS             complement(11751..13169)
                     /gene="araE"
                     /locus_tag="OO572_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6661659.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(13484..14245)
                     /gene="kduD"
                     /locus_tag="OO572_002714"
     CDS             complement(13484..14245)
                     /gene="kduD"
                     /locus_tag="OO572_002714"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6661660.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(14275..15111)
                     /gene="kduI"
                     /locus_tag="OO572_002715"
     CDS             complement(14275..15111)
                     /gene="kduI"
                     /locus_tag="OO572_002715"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6661661.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(15398..16579)
                     /locus_tag="OO572_002716"
     CDS             complement(15398..16579)
                     /locus_tag="OO572_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6661662.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            16834..18063
                     /locus_tag="OO572_002717"
                     /pseudo
     CDS             16834..18063
                     /locus_tag="OO572_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         4858 a         4842 c         4370 g         4561 t
ORIGIN      
        1 attgtttcat actccaggaa gacggtaaac cactctctgc agggcattac acactaataa
       61 caattgaata actcacagtt atgtgcagag ttataaacag aggaaggggt ggatagccgt
      121 ttttattcac cttttctgtg gatagagttg tgaagaacta cggaattact acgggaaaac
      181 ccggagcatt ctgaataagc ccgatataga cattttaaaa tattcattat tagtcaatgc
      241 attacatgta atttgagtaa tgaaaaagag taattcgtga cccaggtcac acctctcatt
      301 tacgggttgg cgaaagatca accaatgccg tgtttatcca cagaatgtgc cactaagtta
      361 agcactgaac cactaaaaac tggagtttcg tcgcacgtca aggctgtaaa tgaaaacagt
      421 agtggaggtt tttcacagtt atcccagctt tctgtggata acatggtgta agatcctgtt
      481 tattttcagt gaccagattt ggaaaaccta ttgcagtgtt gcgcaactcg attaccggca
      541 gcctaaaaag caatataaat cagagaattg aacaacacat gtggaaaaag ttacactgcg
      601 aatattcggc acataattgc tgtttgtttt ttaaccaagg tatcatgaca tgtcccaacc
      661 tcgcccactg ctctctcctc ccgaaactga agaacagttg ttagcgcaag cacagcaact
      721 ttctggttat acattgggag aactggcggc acttgacggg ctggttacgc cagagaattt
      781 aaaacgcgat aagggctgga ttggcgtgtt actggagatc tggctaggtg ccagcgcagg
      841 gagtaaacct gagcaagatt ttgctgctct gggcgtggaa cttaaaacta tccctgtgga
      901 tagtcttggt cgtccgctgg aaacaacatt cgtttgtgtt gcccggttaa cgggtaatag
      961 cggggtgacc tgggaaacca gccatgtgcg ccacaagctc aaacgcgtac tgtggatacc
     1021 ggttgaaggc gagcgcagca tcccgctggc gcagcgtcgc gtaggatcac cgttgctgtg
     1081 gagcccgaat gaagaggaag accgacagct acgcgaagac tgggaagaat taatggatat
     1141 gattgttctc ggtcaggttg agcggatcac cgctcgtcac ggggaatatt tacagataag
     1201 accgaaagca gcgaatgcga aagcgcttac cgaagccatt ggtgcccggg gcgaacggat
     1261 tctgacgctg ccacgcggct tttatttgaa gaagaatttc accagtgcac tactggcccg
     1321 tcattttctg atccagtagc catcgctttg acctgccgct ttccgggcat ataattaccg
     1381 cttcattttt ttggcagggc tttttagatg ttatttgcat ggataaccga tcctaacgct
     1441 tggcttgcgc tcggtacgct gacgctgctg gagatcgttc ttgggatcga caatattatt
     1501 ttcctttctc tggtggtggc aaagcttccc acagcacaac gtgctcatgc gcgccgtctg
     1561 gggttggcgg gagccatggt tatgcgtctg gcgctgctgg catcaatctc ttgggttacg
     1621 cgcctgacaa atccgctttt tacaatattc agtcaggaaa tttctgcccg tgatttgatt
     1681 ctgcttctgg gtggcttgtt ccttatctgg aaagccagca aggaaatcca cgaatccatt
     1741 gaaggtgaag aagaagggct gaaaacacgc gtttcatcat tcctcggcgc tatcgtgcag
     1801 attatgctgc tggatattat cttcagcctc gactcggtga ttaccgctgt gggtctgtca
     1861 gatcacctgt ttattatgat ggcagccgtg gtgattgccg taggcgtgat gatgttcgcc
     1921 gcgcgctcga ttggtgattt tgtcgaacgc catccttcgg taaaaatgct ggcgctctct
     1981 ttcctgattc tggtgggctt taccctgatt ctggaaagtt tcgacatcca cgtaccgaaa
     2041 ggttacatct acttcgcgat gttcttctct attgcggttg aaagcctcaa cttgattcgc
     2101 aacaaaaaga atccgctctg atactccgta cgctctcctg cgggagggcg tactcactcc
     2161 ttttaccctc acagtttaag attttcctgc tttcaagata tataacgtcg gtttataaac
     2221 agactattat cataggtagg ccaaatgagg ataaccggat gaaaaaatgg gcagtaataa
     2281 tttctgcagt cggactggcg tttgctgttt ccgggtgttc cagtgattac gtcatggcga
     2341 ccaaagatgg ccgtatgatt ttgaccgatg gaaaacctga aattgatgat gataccgggc
     2401 tggtgagtta tcacgatcag caaggtaacg cgatgcaaat taaccgtgat gatgtttcgc
     2461 aaattattga acgttaacaa ataaggtcag catccggctg gccttaagat ttttctcttt
     2521 cccttttccc ttccctctgc catttttata ttccttatgt cgtgattata aaaaggaaac
     2581 ggctatgcaa tatcaccgta taccccacag ttcgctggaa gtcagcacgc tggggcttgg
     2641 cacgatgacg tttggtgaac agaacagcga agccgacgcc cacgcacaac tcgactatgc
     2701 cgtcgctcag ggcattaacc ttatcgacgt tgccgaaatg tacccagtac ctccgcgccc
     2761 cgaaacgcaa gggttaaccg aaacctacgt cggcaactgg ctggcgaaac atggcagccg
     2821 cgaaaagtta attatcgcct ccaaagtgag cggaccgtcg cgcaataatg acaagggcat
     2881 ccgcccggat caggcgctgg atcggaagaa tatccgcgaa gcgctgcatg acagcctcaa
     2941 gcgcctacag actgattacc tcgatcttta tcaggtgcac tggccgcagc gcccgaccaa
     3001 ctgcttcggc aaactcggtt atagctggac ggattctgcg cctgcggttt cgctgctgga
     3061 tacgctggac gcactggcag agtaccaacg cgcgggaaaa attcgttata tcggcgtgtc
     3121 gaacgaaact gcatttggcg taatgcgcta cctgcatctg gcggacaaac acgatctgcc
     3181 gcgtattgtc accattcaga acccttacag tctgttaaac cgcagttttg aagtaggtct
     3241 ggcagaagtc agccagtatg aaggggtcga actgctggcc tattcgtgcc tgggtttcgg
     3301 cacgctgacc gggaaatatc tcaatggtgc aaaacccgct ggcgcacgta atacgctctt
     3361 tagtcggttc acccgctata gcggtgagca aacgcaaaaa gccgtcgcgg cgtatgttga
     3421 tatcgccaga cgtcatggcc ttgatcctgc tcagatggcg ctcgcgtttg tacgccgtca
     3481 accgtttgtt gccagcactc tgctgggcgc aaccacgatg gatcagctga aaactaacat
     3541 cgaaagtttg catctggagt taagcgaaga cgtattagct gaaattgaag cggtacatca
     3601 ggtttatact tatcctgcac cataaagatt aacgccggtt aaaaccggcg ttaaatatta
     3661 atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaatagcg caccgaagcc
     3721 aatgccaatg ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat
     3781 caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac
     3841 gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccag
     3901 cacgccaatc agcatcaaca aggcataggc tggcagcagc tcgtgttgca gggagaaaat
     3961 cggtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt
     4021 aaccagcttc gctgccgcac ctgcgccaac cacgatacca atcgctacca tcgcgttgag
     4081 ataggtcggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag
     4141 gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt
     4201 ttcaccattg caccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgttgatgag
     4261 attccaggac tgccccggac gcgccgccag tttgggaatg tagatattgg caacgaccgc
     4321 accaccgtag gccagtgcgc atgcggccag agcgaccagg acatgccagt cagccagcac
     4381 accaccggct acggaaccga gtaaaatcgc cgctatggca gaagcttcca ttaaaccgtt
     4441 agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
     4501 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
     4561 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
     4621 gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga atgtaagcac ctacaaacac
     4681 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
     4741 ggcaaacagt agcgcgttat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
     4801 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
     4861 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
     4921 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
     4981 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
     5041 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
     5101 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
     5161 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
     5221 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
     5281 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
     5341 cgccctcttc gataccaggg accgacaaca ggcgtgcatc cattcctggt agaatacgcc
     5401 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg
     5461 tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
     5521 tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
     5581 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc
     5641 ggtcatacac caagtccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt
     5701 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
     5761 gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
     5821 cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg
     5881 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc aggcgcggca
     5941 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
     6001 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
     6061 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca
     6121 gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgc gcgatggccc
     6181 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
     6241 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
     6301 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
     6361 ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata cgcgcttcca
     6421 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
     6481 ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg aacaagcgac
     6541 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
     6601 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
     6661 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
     6721 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
     6781 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
     6841 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
     6901 gcgtaattag tacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat
     6961 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
     7021 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
     7081 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
     7141 cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga
     7201 ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg
     7261 tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg
     7321 tgtaattcat gtgctcaaca cgaagttgac ttcacctatc aatacctctg ggatcaccac
     7381 tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt
     7441 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca
     7501 cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaacaatgta
     7561 agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
     7621 cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcttc
     7681 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
     7741 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
     7801 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
     7861 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
     7921 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccagatg ccgatttagc
     7981 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
     8041 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
     8101 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
     8161 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattccgg ccaatgatcg
     8221 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
     8281 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggcgc
     8341 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
     8401 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
     8461 cgtaacgatg gcgacccagg ctgccgaact ggctttggca ctggcggata atcgccctct
     8521 cccggaaatt actaatgtat ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
     8581 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
     8641 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
     8701 gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc
     8761 ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa
     8821 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggc ccggcgacga
     8881 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
     8941 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca
     9001 caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact
     9061 gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag
     9121 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
     9181 caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
     9241 cgaattcgat aacctgacgc accatagcac cacacacctg ctccagatgg gcataatcaa
     9301 cgccagaacc aatgtgcatg tgaatgccga ctagctgcag atgatgacgt tgtatcacgt
     9361 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
     9421 tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc
     9481 ctggcgaaac ctggcccagt tggtcgagcg tatcaacaga acccgcattc accggaattt
     9541 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
     9601 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
     9661 agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac
     9721 aggctttctg tgcaaagcgc accacttcaa actgtttcag cgctgcaatc tgccgacgaa
     9781 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
     9841 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
     9901 gagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
     9961 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    10021 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag
    10081 cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg
    10141 tgatcgggct gaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac
    10201 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg
    10261 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    10321 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    10381 taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    10441 atttaggact cactgagaca ctccatacgc ctacgggaac agaacgtacc gaattactct
    10501 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    10561 cgccggatga ttttcagggt gagaattaca tcagcctttc ccgtactgac agctatcgcc
    10621 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    10681 acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtatttcg gtggttaacc
    10741 cgctcaccgc actagattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg
    10801 ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggttcagg
    10861 cgtttagtgg gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt
    10921 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc
    10981 aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa
    11041 aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    11101 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    11161 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    11221 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    11281 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    11341 ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc
    11401 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    11461 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca tttgcgcaga
    11521 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt
    11581 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    11641 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccaat
    11701 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg
    11761 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    11821 ttggtttccg gaatgagcca gaaagtaata cccacaaacg caatgttcag cgcagtgtag
    11881 agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggc cgcgccgata
    11941 atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc
    12001 agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg
    12061 gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg
    12121 ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa
    12181 ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc
    12241 gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    12301 aagcccgcca ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac
    12361 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    12421 ttaaacagtg cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct
    12481 tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga
    12541 cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc
    12601 agaactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac
    12661 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    12721 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    12781 gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg
    12841 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    12901 tatttacgcc ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg
    12961 agcatcatgc tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta
    13021 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    13081 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cacgcaaaga acgtggcgtt
    13141 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacattgt
    13201 gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa atatggatta
    13261 aattgctgcg acatgccgtt atgtgatgga tattccaatt ttaaaattaa gttgaattat
    13321 tgagattatt attaacctcc taattttaaa gcagataaaa ttcataaagt tcattaattg
    13381 ataattgata tggattattt cataaccatg atatggatta tgatgatcta gagatataaa
    13441 aaaccctgcc atgcggcagg gtcataaaag taagaggaat gaattaacgc gccagccaac
    13501 cgccatccac agcaatggta taaccattta cataatctga agcgctggag gcaagaaaca
    13561 ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg cgatcgagaa
    13621 tttccgcgct acgttgttca tctgcccgca gttgttgggt attgttggtc gccatgtaac
    13681 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgtttgcc atcaatcgcg
    13741 tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
    13801 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
    13861 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc
    13921 agtctttttc gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca
    13981 ggatatcaat atgaccaaat tcagctaccg cgcgatccag cagtccagga atgccatcaa
    14041 tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgctcga
    14101 tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc
    14161 ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca actttacctt
    14221 ggagagaaaa tgcacttaaa atcataacaa caccttattc gtttatgccc acaactagcg
    14281 caaatcttta acgcccacat ggtccatatc attaaagacc tggttttcac cgaccatgcc
    14341 ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat
    14401 caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc ccatcatgtg
    14461 gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg
    14521 ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact
    14581 caattgacag gtttccagta catccgggac aaaatatttg ttaatcgtgc gacggttact
    14641 ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt ttttggtggg
    14701 atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc gccggagtgg cggtatcaat
    14761 actggcaaag acaacttctt ttgcaccttt accaacatac agggcgtcgc ggtgaccgat
    14821 ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa
    14881 ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga
    14941 aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac
    15001 catggtgtac tcatcggcga caaatacctt ttcaaccaaa aattcattgc gcagcccttg
    15061 ggtatccagc gtttttgcgt gcgcaccgtg gatgctctgt cttacgtcca catcaacctc
    15121 cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta
    15181 taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca atgttttatt
    15241 tttaatttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa
    15301 aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat
    15361 gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca
    15421 atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg gcattacgtt
    15481 tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac
    15541 cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca agcatcttgc
    15601 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
    15661 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
    15721 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
    15781 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
    15841 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
    15901 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
    15961 tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
    16021 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
    16081 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
    16141 catcatgcac cagactgtca accaactggc tattgccaag ctgttcaccg gtgcggctat
    16201 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc acctgcgatg acaatatcag
    16261 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagaccg gaaccgcaaa
    16321 cgtcattaat cgtgattgca gaaacgctgt taggaagacc acctttaata gccgattgcc
    16381 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
    16441 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
    16501 cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga
    16561 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
    16621 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
    16681 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
    16741 atgggtagat agggcgttaa tctcccatac ttaacttggt ttatggtaaa ttgccctcca
    16801 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
    16861 actctgtgaa gtttcgcgct ctatggcaca gccgttgatg caggcacgct attccttcct
    16921 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
    16981 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcaaa aacatcagca
    17041 ggtgaaggga taacgggcgc ggtaacacac tactatggca agaagattgg taatctgatt
    17101 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
    17161 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
    17221 gtgagtctgg gtgttgtatt aattctgaat ctcatttttc tgatgggacg tcatgccact
    17281 attcgggtaa tgggattttt ggtattccca ttgattgcct atttcttatt tctttccatt
    17341 taccttgtcg gtagttggca acctgatcta ttaacaaccc aggtagagtt caatcagaat
    17401 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
    17461 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
    17521 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
    17581 tttagctgcc tgctttctat tccatcttcg tatattgaag cggctaaaga agaaggggtc
    17641 accattttat cggcgctttc tatgctgccg aacgccccag catggttgtc aatttccggg
    17701 attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt
    17761 gaaggtgcca cagaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
    17821 gcatttaacc acgcactatc aattatgttg gtatcgctga ttaccttcat tgtttgttgc
    17881 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
    17941 cttttcatca tgcctacgct gtcaacgtat ctcatcccgg cgcttaaacc ctggcgttcc
    18001 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
    18061 taatatccgt tgtggatggt tcaaaattgc tacaattttg aaccattcca tttttcataa
    18121 tattcatttg ttattattcc gccctatcac ttctctcgtc atatcctaga attatcagtt
    18181 agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat gcataactta
    18241 gattcgcaag atatcttctg gtcattcaaa aacaatttgc gatatagata ttctcatacc
    18301 atgagatagt tatctggacc acgttcgacc atgcaagatt aaatcagtga aacataatca
    18361 tatttaataa cgagaaatgc atttttaaat atcttaatca gaattatagc aaatacagat
    18421 taatcgcaat ttttcccagc aaaattacaa aatcaaggtg tattgacatc atcaatcacc
    18481 aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt
    18541 tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca
    18601 ccgcgtttgc agtactgcgc aaactcgtgt t
//
LOCUS       DAJTYL010000082        18419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYL010000082 DAJTYL010000000
VERSION     DAJTYL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.082"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(58..975)
                     /gene="lpxC"
                     /locus_tag="OO572_002718"
     CDS             complement(58..975)
                     /gene="lpxC"
                     /locus_tag="OO572_002718"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6661663.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(1076..2227)
                     /gene="ftsZ"
                     /locus_tag="OO572_002719"
     CDS             complement(1076..2227)
                     /gene="ftsZ"
                     /locus_tag="OO572_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6661664.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(2288..3550)
                     /gene="ftsA"
                     /locus_tag="OO572_002720"
     CDS             complement(2288..3550)
                     /gene="ftsA"
                     /locus_tag="OO572_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6661665.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(3547..4377)
                     /gene="ftsQ"
                     /locus_tag="OO572_002721"
     CDS             complement(3547..4377)
                     /gene="ftsQ"
                     /locus_tag="OO572_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6661666.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(4379..5299)
                     /gene="ddlB"
                     /locus_tag="OO572_002722"
     CDS             complement(4379..5299)
                     /gene="ddlB"
                     /locus_tag="OO572_002722"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6661667.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(5292..6767)
                     /gene="murC"
                     /locus_tag="OO572_002723"
     CDS             complement(5292..6767)
                     /gene="murC"
                     /locus_tag="OO572_002723"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6661668.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(6821..7888)
                     /gene="murG"
                     /locus_tag="OO572_002724"
     CDS             complement(6821..7888)
                     /gene="murG"
                     /locus_tag="OO572_002724"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6661669.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(7885..9129)
                     /gene="ftsW"
                     /locus_tag="OO572_002725"
     CDS             complement(7885..9129)
                     /gene="ftsW"
                     /locus_tag="OO572_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6661670.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(9129..10445)
                     /gene="murD"
                     /locus_tag="OO572_002726"
     CDS             complement(9129..10445)
                     /gene="murD"
                     /locus_tag="OO572_002726"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6661671.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(10448..11530)
                     /gene="mraY"
                     /locus_tag="OO572_002727"
     CDS             complement(10448..11530)
                     /gene="mraY"
                     /locus_tag="OO572_002727"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6661672.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(11524..12882)
                     /gene="murF"
                     /locus_tag="OO572_002728"
     CDS             complement(11524..12882)
                     /gene="murF"
                     /locus_tag="OO572_002728"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6661673.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(12879..14366)
                     /gene="murE"
                     /locus_tag="OO572_002729"
     CDS             complement(12879..14366)
                     /gene="murE"
                     /locus_tag="OO572_002729"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6661674.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(14353..16119)
                     /gene="ftsI"
                     /locus_tag="OO572_002730"
     CDS             complement(14353..16119)
                     /gene="ftsI"
                     /locus_tag="OO572_002730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6661675.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(16135..16500)
                     /gene="ftsL"
                     /locus_tag="OO572_002731"
     CDS             complement(16135..16500)
                     /gene="ftsL"
                     /locus_tag="OO572_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6661676.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(16497..17438)
                     /gene="rsmH"
                     /locus_tag="OO572_002732"
     CDS             complement(16497..17438)
                     /gene="rsmH"
                     /locus_tag="OO572_002732"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6661677.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(17440..17898)
                     /gene="mraZ"
                     /locus_tag="OO572_002733"
     CDS             complement(17440..17898)
                     /gene="mraZ"
                     /locus_tag="OO572_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6661678.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         4368 a         5394 c         4440 g         4217 t
ORIGIN      
        1 tggccggaga gagtgccaga tttgccagtc gaattttata cgacagtata aatgtcgtta
       61 tgccagtaca gctgaaggcg ctttgaaggc caacggcagt tctgcgtcgt cctggaaggt
      121 cacatattcc caggcttcct gtttcgccag gacagcctgc agcagtttgt tattcagtgc
      181 atgaccggat ttataagcgg taaatgcacc aataatattg tgaccacaca tgaacaagtc
      241 accgatcgca tcgagcattt tgtgacgcac aaattcgtct tcaaaacgca ggccgtcttc
      301 gttcagtacg cgataatcgt caacaacgat ggcacaatcg aagctgccgc ccaggcacaa
      361 accacgggac tgcagatatt cgatatcacg catgaaaccg aacgtacgcg cacggctgat
      421 ctggcgcata aacgcatcag cggagaagtt catcgcatag cgctggttgc tggaatcaat
      481 agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa ccattgtacg gcttaaattc
      541 agcccactta tcgccatctt cgacacgaac agtctctttg atgcgaacaa attttttggc
      601 gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat acaaacggag cggcgctgcc
      661 gtccatgatc gggatttccg gcgcgttaac ttcgataaca atgttatcga tgcccaggcc
      721 cgcgagagca gcattgaggt gctctacggt tgaaatccgt acatcatgct cgttgaccag
      781 acacgtacag agcatggtat cacgcacaga tttggcatcg gccgggaaat ctaccggtgg
      841 attcaagtcg gtgcgacgat agatgacccc ggtgttggcc ggcgcagggc gtaacgtcag
      901 ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc tgaacgatac gtttaagtgt
      961 cctttgtttg atcatcgtat tatctcgcca aattacctat ccaaccgaag tgtactatac
     1021 attcggcggg ccagtttagc acaaagagcc tcgaaaccca aattccagtc aattcttaat
     1081 cagcttgctt acgtaggaat gctgggatat ccagataatc cggctctttc gcagtttgcg
     1141 gcgcattgtc attcactact ttagcaaccg gcttctgctc ctgggtcagc ggagccatcc
     1201 catgctgctg gtagcgatcc atcactggct gctgaacctg cttattggtc accagagtga
     1261 tttcaggacg tttgtccatg ccgatacctg tcgcaacaac ggttacgcgc agctcgtcat
     1321 tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc gttgtcggaa gcaaatgcac
     1381 ggatggtgtt acctaccgtt tcgaactcat ccagacgcag gtcgaagccc gccgtgatgt
     1441 taaccagcac gccgcgcgcg ccagacaggt cgatatcttc cagcagcgga gaagagatag
     1501 ccatttcagc agcttcttcc gcacggtctt caccgctcgc cacgccagaa cccatcattg
     1561 cgtagcccat ttcagacatt acggtgcgta cgtctgcaaa gtccacgttc atcaaacccg
     1621 gacgagtaat cagttcggcg ataccttgca cagcgccttt cagtacatcg ttcgctgcgc
     1681 caaacgcatc cagcagggag ataccgcggc ccagaacttt cagcagtttg tcgttcggga
     1741 tagtgatcag agagtccaca tgcttggaca gttcagtgat cccctgctcg gcgaatgcca
     1801 tacgcttctt gccttcaaag ttgaaaggct tagtgacgac agcaacggtc aggataccca
     1861 aatcttttgc cacttcagcg acgactggtg ctgcacctgt accggtacca ccacccatac
     1921 ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc acgcaatgca tcgcgatcct
     1981 catcagccgc attgcggcca acttctggat tagcgccagc gcccagtcct ttggtgatac
     2041 cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg cagcgcttgt gcatcggtat
     2101 ttaccgcgaa gaattcaaca ccttcaatac gctcgcgcac catgtgttca acagcattac
     2161 cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc attggtaagt tccattggtt
     2221 caaacatagt ttctctccga tttgtgcctg tcgcctgagt ccgtaatcat cgtcggcctc
     2281 ataaaaatta aaactctttt cgcagccaac tattgagtcg cttgatccac gagccaactg
     2341 atgctgtaac acgtttttct acttcagctt caccgttaag atgtgactct ttcccatagt
     2401 gaagcaatcc caccgccgtc gaataatacg gctcctgagc ataatccgtt aaaccggtaa
     2461 tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac gcgctgagca caggctgcaa
     2521 gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc tgccgccagg tgatgtttaa
     2581 ccccttgttg gcgaagcttt tcctgcaact gcaatatctc ttcgttgacc aggttgagca
     2641 actcggtata gcgcggctcg atcacctctg ccagggtctg acgttgcaga ctccgcggcg
     2701 gacgaccgcc tacgctcggc acttccacgc tctcatcttt tccaacgatg gaacccagcg
     2761 cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg cggcgtgcca aaggcgtaag
     2821 cgatatcact ggtcacgaca ttgccagcat aaggaattac ctttgtgtgg cgcaatgccc
     2881 caccggtata aacggcgata tccattgtac caccaccgat atcgacgacg cagacaccca
     2941 gttcacgttc atcttccgtc aataccgaat aacttgatgc cagtccggca aatatcagtt
     3001 ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat gtttttcgcc atatcgttgt
     3061 gacatgtgat caggtgcact tttgcctgca tccgcacacc cgaaagtcct accggattct
     3121 tgatcccttc ctgatagtca atcgcatact cttgcgggat cacatgcagc acacgatgct
     3181 catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt ttccacatct tcttgcgtca
     3241 cttcttcttc agaaataggc accataccaa tttcattctg gcagctgatg tgcttaccag
     3301 aaagcgccag atataccgaa gagatctgac aatctgccat caattctgcc tggtcaatgg
     3361 cgcgttgtac gcacttgacc acggattcga ggtcgttcac cccgccttta tccataccac
     3421 gtgacgggca gctgcccacg ccaatgatat tgaccatacc gtcgggcaga acttccccta
     3481 ctaaagcggc aaccttcgcg gtaccaattt ccagtcctac taccagtttt ctgtccgtcg
     3541 ccttgatcat tgttgttctg cctgtgcctg attttgttgc tgagtagatt cctctggcgg
     3601 caagggcgcc cagcctactg ccgctccaga gtcataacgc aaatcaacgt agctaatccg
     3661 tttgccatcg gtttgcgcct gctgctgtaa aaccggataa agttctacaa agcgaaccaa
     3721 acgtttcatc gtatcgcccc ggccaagatt gagcttaata tcgttattca gcgtcaactg
     3781 ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta aatctgtcct ttgccagcat
     3841 ctgccccatt tcgcgatagc cctgcaacac ttcattagcg ctgccttccg ggccatacag
     3901 cattggaagc acctgcttgc tggtgcgatc tggcggcacg ctgaaggtgt ttccttccgc
     3961 gtctaccatg tgttgatcat tccaccgcgc aatcggcaca tattcaacca gatgaatctt
     4021 caattcatca ggccactgct ttctgacgct cacctgctta atccacggca ggcgttgttc
     4081 tatttgcgtc tggatgatgt tgacatcctg ggtcataaag gtacccggct cacccaatgc
     4141 caggatcgac tgccggatat cgtcattacg cgtgtaatgg cgttcaccgg tcaacaccag
     4201 ctttgagagc ggcaggcgtt gcgcatcttc catccagctc aacacgaccc agccgctcac
     4261 caacactgtc gttaaaacgg tcagcaggaa aaggatcccc gccagacgcg ttccattatt
     4321 gcggcgagaa gaaacctctt cttcgctgtt tcgcgtgttc agagcagcct gcgacatatt
     4381 agtccgccag ttccagaatt cgtactacca actgcgagaa gctcatacct gcctgacgtg
     4441 ccgccatcgg caccaggctg tggctggtca tacccggtga ggtattggct tccagcagat
     4501 aaaactgtcc atcactgtcc agcataacgt caatacgtcc ccatcctttg caacctaacg
     4561 tcgtccatgc tttcagcact aatgcctgca aattggcctc ttgtgacgct tccagacctg
     4621 cggggcagaa atactgtgtc tcatcagaga gatacttcgc ctcataatca tagaaggttc
     4681 cggacggttg aatacgtatt gacggtaaaa tttcttcacc gagtatcgca accgtgaact
     4741 ccggcccact tagccatttt tcaatcaata cttcttcatc gtgctgaaat gccaatctta
     4801 atgcatcttg tagagcattt tctgctacta cttttgacat tcccacactg gaaccttcgc
     4861 ggctcggctt aacgataacc ggcaaaccca gagcagaaat ttctgctaac tgcttatcgc
     4921 tcaggccttt ttcaaactct gcgcgggtta acgctaccca cggcgcgacc ggtaaaccgg
     4981 caccttgcca cagaagtttg ctgcgtagtt tatctattga aagcgcagat gccatcactc
     5041 cgctgccggt ataaggcaag cccatcagct cgagcatgcc ctgcagcgta ccatcttcac
     5101 cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc catcgacttc agttgcgtca
     5161 cgtcgacttc tttcgggtcg acaggatacg catcaatacc gccttcacgc agcccggcta
     5221 acaccgctgc gccagaattc agagaaactt cccgctcagc ggaagtcccg cccaacagga
     5281 ccgcgatttt atcagtcatg ttgttcttcc tccggagttt gcggcttcag tttgatttca
     5341 gctaaagaac gggcaatttt tccgatatta ccagccccct gaacgagaat caggtcgtta
     5401 ccggttaata ccggtgccag catctcggct acctgcgcag ggtccggcac cagaatggga
     5461 tcaattttcc cacgtccacg aattgtgcga cacagcgaac ggctgtccgc tcccggaatt
     5521 ggcgcttcgc cagccggata cacttccagc atcaacaggg tatcaacctg cgtcagcaca
     5581 ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac ggtgcggctg aaacagcatt
     5641 accaggtttt tatccggcca gcctgcgcgc gccgctttaa tggtggcgtc aacttccgtc
     5701 gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc ttttaccatt cactggctcc
     5761 agcgggaatt caccgaggaa atcaaaacgg cgaccagtcc cctggaagct ctccagcgca
     5821 cgcaaaatag cctcgtcgtc aattccctct tccgtagcaa ccgcaaccgc agctgcggcg
     5881 ttcagcgcgt tatgacggcc tggcgcattc agggtgacgc acatcggctc tttgtcctga
     5941 cgcagcagcg taaagtgccc ctgcgggcca atctgctgat aatcttctac acgcacgtcg
     6001 gcatcttcgc tgaagccgta agttgtggtc tgacgtccca cacgcggtaa caattcgcgg
     6061 atcaccggat catcaacaca catcaccgca cgaccgtaaa aaggcaggtt gtgcagaaaa
     6121 ttaataaagg tctgttttaa attctcaaag tcgccctggt aggtatccat gtggtcggct
     6181 tcgatattgg tgacaatcgc caccatcggt tgcagatgca ggaacgatgc atcactctca
     6241 tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg catgaacccc cgccgctttt
     6301 accagcccgc cgttaacgaa ggttgggtcg agccccgctt ctgcgtagat gctggaaacc
     6361 atcgcggtag ttgtcgtttt gccgtgcgtt cctgcaatgg cgatgccatg acgaaaacgc
     6421 attaactcag ccagcatttc ggcacgacgg atcaccggaa tacgcgcttc atgagctgcg
     6481 acaatttccg ggttgtcggc agaaatggcg ctggaaacaa cgaccacgct ggcatcacgt
     6541 acgttttccg ggcgatggtt gaaataaatc gtcgcaccca gattcagtaa ctgctgcgtg
     6601 accggatttg gcgctaaatc ggaaccactg atctgataac cttcattggc cagaacttcg
     6661 gcaataccgc ccataccggc accaccaatg ccgacaaaat gtatgtgccg aacgcgacgc
     6721 atttcgggca cgatggaacg cagttttgcc aattgttgtg tattcattct ttacgccatt
     6781 aacttcttaa attcatacga tgcaaaaggc atcgctacaa ttacgcccgg gcagcccggc
     6841 tcacttcatt tgccactcgc tcggtggcat ccggaatgga tgcagcgcgg gcgcgttctg
     6901 ccatggttaa taaggtttct cgcgaccacc cggccagggt gttggcgaca gcatccacgc
     6961 taagctgtgg ctgctcgata attttggctg cgcccgcttt ttccagcggt agcgcattcc
     7021 agtattgctg gcggtcttta tgttgaaacg gcacaaacaa cgccggtagt cctgccgcgg
     7081 cgatttcact cacagttaaa gcaccggagc ggcaaacgac gacatccgcc cacgcatacg
     7141 ccgccgccat atcatcaata aattcagtca ctttatgctg cggttgcccc gcttcggcat
     7201 acgcctgttc aacggattgt tgcgaacctt tgccgctctg atgccagata gtgactgaat
     7261 cacccagttt cgcagcaacc tgcggcattg tctggttaag aatgcgtgcg ccctgggaac
     7321 cacccactac caacacacga accggacctt cacgtccagc caaacgttgc tgcggcaacg
     7381 gcagcgccag cacatcggta cgcaccgggt tacccaccac ttccgcatta gggaaagcgc
     7441 ctggaaacgc ctgcatcact ttggtagcaa tcttcgccag ccatttattg gttaagcccg
     7501 caataccgtt ttgttcatga agtacaaccg gaatgcctaa cgaccacgcg gccagaccac
     7561 ctggacctga tacatagccg cccataccga gtaccacgtc aggtttgtac gctttcataa
     7621 tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg cgcagctatc agtgctttta
     7681 taccttttcc acgcaaacca gagatacgaa tgaaatcaat ttcgatgcca tgttttggca
     7741 ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg aacttgccaa ccctgagcca
     7801 ttagatagtg cgcaaccgcc agtcccggga atacatgtcc accggttccg cctgccatca
     7861 ccattaatcg ctttccttga ccactcatcg tgaacctcgt acaaacgcct gcgctttctc
     7921 cagacgcgtt tcataatcaa tacgcaacag catcatgatg gctgtcgaca taatcagtaa
     7981 gctcgaacca ccgtaactga ttagcggcaa tgtcagacct ttggtcggta acatccccgc
     8041 cgccgcgcct acgttaacca gcgcctggaa gctaaaccag atgccaatag aacaggcgag
     8101 aaaaccggaa aaacggtggt caatttctaa tgctttgcgg ccaatcgaca tcgcacgaaa
     8161 agcgacgaag aataccatta aaagtgctag taccacaccg acataaccta attcttcgcc
     8221 gataatggcg aaaataaagt cagtgtgcgc ttccggcaga tactccagtt tttgtaccga
     8281 attacctaaa ccttgccccc aaagttcgcc gcgaccaaac gccatcagcg attgcgttaa
     8341 ctgatagccg ctgccaaagg gatcttccca cgggttccag aatgcggtaa cacggcggat
     8401 acggtacggt tcggcgagga tcagcaacac aaccgctgaa atgcccatac cgataatggc
     8461 aatgaactgc cacaatttcg ctcccgccag gaacaacatc gccagcgtag tcacaaacaa
     8521 caccaccacc gtaccaaggt ctggctgtgc cagcagtaac actgccaaca ccagaatcac
     8581 gcccatcggt ttcaggaagc cgcgcaggtt attacgtact tcgtcgcctt tacgcaccag
     8641 atagttggcg atatagcaaa acagcgacag ttttgtcagc tccgcaggct ggatacgcag
     8701 caaaccgaga tcgatccaac gcgatgcccc tttaacagcg ctacccacta ccagcacgat
     8761 catcagcagg atgatagatc cgagcagcat cgtggcactg tagcgttgcc agaactccat
     8821 cggcagacgc agcgtaatga tcgccagaat aaacgccaaa atcagataga caccatcacg
     8881 cttcgcgaag aagaacggat cgttggttaa gcgttgccct atgggcattg acgccgaggt
     8941 caccatgata aagccaatcg ccgcgaggcc gaaggtcagc cacagtaagg tgcgatcgta
     9001 catgatcagg ctgtcggtat ctttttcccg cgagcccatc acccagccct ttagcgccgt
     9061 ggagatccag accaggatac tgaatcctgg caggcgcggc attttcaggc gagggagaga
     9121 taaacgcatc aacctaactc cttcgccaga cgggcaaact cattgcctcg ttgttcaaag
     9181 ttcttgaact gatcaaggct ggcacaggcc ggggagagca gaaccatatc gcccggctga
     9241 acacgcggag ccagcaagcg catagcctgt tccatagttt cggtttgttc tgccacttcc
     9301 gggcgtagcg ccgccagctg cgcgccgtca cgaccgaaac aatacagacg tacgttatcg
     9361 ccattcaggt aacgcaccag tgggctaaag tccgccgatt taccatcgcc gcccagcaac
     9421 aaatgcagcg tgccgtctac gtgcaggcca ttcagcgccg cttccgtact gccgacgttg
     9481 gtcgcttttg aatcgttaat ccagcgcaca ccgttatgct ccagcaccac ttcaaagcga
     9541 tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc tggcgcgcgg taaccctgcg
     9601 gcatctgcca gcgccagcgc caccagcgca ttggtgtagt tatgctgccc ggaaagtttc
     9661 atctctttca cattcagcac tttctcgcct ttaacccgca gccaggtttc gccctgctga
     9721 tgattcaggt gatagtcacc catgttgacg ccaaagctga cgcagcgttc atccgcaccg
     9781 cgaatcggca ttgttaaggc atcatcagca ttaaccacgc aaactttcgc gttttcgtaa
     9841 atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg ggtagcgatc catatgatct
     9901 tcagtcacgt tcagaatggt cgctgctacc gcctgtaagc tggaggtggt ttccagctgg
     9961 aagctcgaca gttccagcac gtacagttca cactcatcat ccagtagcat caacgcaggc
    10021 aggccaatat tgccacccac accaacgtta acccccgccg ctttcgccat ttcacccact
    10081 agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg ccacaatcgg tgcttgtgct
    10141 tcgcgacaaa acagctcgat atcgccaacg atttcgattc cggcatcagc ggcagcgctt
    10201 aaggatggat gcgccagtgc aataccggga ctggcgacaa tcagatctgc cgccatcagc
    10261 cattcatcat tcagactgcc cgtgtggcgt tctacggctt cgggtaattt atccaggcca
    10321 ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac cgcgagcgag gaaaaagtcc
    10381 acgcaggaaa gcccggtgag gcccaggccg ataatgacaa catttttacc ctggtaatca
    10441 gccatgatta acgtaccttc agcgttgcca gaccaatcag gaccagcatc agcgaaataa
    10501 tccagaaacg cacaatgacg cgcggttccg gccagccttt cagttcatag tggtgatgaa
    10561 tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa ggagccgacc tgcaggatga
    10621 cagaaagcgt ttctaccacg aacacgcccc ccataatcac cagcaggaat tcctgacgta
    10681 gcagtacggc gataatgcct aacgcaccac ctaacgccag cgaaccgaca tcgcccataa
    10741 agacctgcgc cggataggtg ttaaaccaca ggaagcccag tcctgccccg actatcgcgg
    10801 tacagacaat aaccagttcc ccggcgtgtc gcagatacgg tatgtgtaaa tagctggcaa
    10861 agttcatgtt gccggtcgcc cacgccacca gcgcaaaacc accggcgaca aatacggtcg
    10921 gcataattgc cagaccgtca agaccatcgg tcaggtttac cgcgttgcca gtacccacaa
    10981 tgacgaagta agccagcaga atgtagaaca gccccagttg cggcatcaca tctttaaaga
    11041 atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc aaggtacaaa gcgaaagcaa
    11101 cacccagcgc aatgaccgac atccagaaat atttccaacg agcgatcaac cctttggtgt
    11161 ctttacgcac cactttgcga taatcatcaa caaagccaat aacaccgtaa cctaccagca
    11221 ccaccaacac gcaccagacg tacggattgg acgggtaagc ccacagcagc acggagatca
    11281 caatcgctgt caggatcata atcccgccca tggtcggcgt accgcgcttg ctgaagtgtg
    11341 attcaggacc gtcgttacgc actacctgac caaaggaaag tttttgcaaa tgagcaatca
    11401 tgcgcgggcc catccacaat gagatgaaca gcgcggtcag caggctgacg atggcgcgaa
    11461 acgtcagata ggaaaagacg ttaaagccgg aataatattt gaccaaatgt tcggccagcc
    11521 aaactaacat gtcccattct cctgtaaagc gcgtactacc tcttccatgg cagcactacg
    11581 tgaaccctta actaaaatcg taattacctg ttgctcagca atcagtgatt taagacgcgt
    11641 aattaacgca gttttatcag caaaatgttc gccaacgccg ctggcggtgc tgatagcatg
    11701 gctttgttta cccatgctta acacgcggtc aataccagca gcttttgccg cctcacccac
    11761 ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc atatcgccca ccaccagcac
    11821 gcggtagccc ggcatttcag ccagtacctg aacagctgcg gtcattgaac cgacattggc
    11881 gttgtaggag tcgtcgagca gcaactggtt ttctgccagt tggatgggga acagacggcc
    11941 aggaacagct ttcagatttg ccagccccgc tttgatagca tcaagcgttg cgcccacgga
    12001 catggagagc gcagcggctg ccagcgcatt cgcaatattg tgacgccccg gcaacggcag
    12061 cagaacatcg acgctaccgg ttggggtttg tagggtaaat tccataccgt gcgaggtcac
    12121 atggatattg gtggcggtga aatcgctgtt ggcggcattg ggtgagaaac gccacacttt
    12181 gcgtgagcca attacgctct gccagttcag ccagtcgttg ttgtcggcgt tcataatggc
    12241 gataccgttt tccggcaggc cgctaaagat ttcacctttc gctttcgcga cacccgcaag
    12301 cgagccaaaa ccttccagat gcgccgctgc caggttgttg accagcgccg cttccgggcg
    12361 agtcagactc acagtccagg caatctcgcc ctgatggttc gcgccaagtt caataactgc
    12421 gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt acaccgatgt cgttgttgag
    12481 attgcctgcc gtataaagcg tgttgccgca ctggcttaaa atcgccgccg tcatctcttt
    12541 aacggaggtt ttgccggagg accccgtcag agcaaccacg agcgccggaa cttgctggcg
    12601 aacccatgca gccagttcac caaacgccag acgcgtatcc ttgacgatta actgcggcag
    12661 gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg ccgccagctt tcgcctggtc
    12721 ggcaaaatcg tgggcatcaa aacgttcacc tttcagggca acaaacaggc agcccggcgt
    12781 cagttttcgg gtatcagtgg ttacagcatc aagggtgata tctgcacctt gcagttcacc
    12841 gttgagaata tcggtaagtt ggctaagggt tacgctaatc atgcaatccc ccccagcaga
    12901 cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat tgccaacaat ctggtaatcc
    12961 tcatggcctt tgcccgcgac cagtaccaca tcattctctt tagcctgcat aacggcgcaa
    13021 gtcaccgctt cagcacggcc ttccatcact ttggcatgtc cggcatctaa cattcccgcc
    13081 agaatatcgt tgatgatggc acgcggttct tcggtacgcg ggttatcgtc cgtcaccacc
    13141 gccacgtcag caaactcttc ggcaattgcg cccatcagtg gacgcttacc tttatcgcga
    13201 tcgccaccac agccaaagac gcaccacagc ttgcccgcac agtgcaggcg cgccgcctgt
    13261 aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca ccaccaccgt cggtttgcct
    13321 ggcgcagtga acacttccat acgtccgcaa accggttgca gacgcgcggc ggttttcagc
    13381 agatcagcca gtggatagcc cagcgccagc agtgttgcca gcgcgagcaa caggttgctg
    13441 acgttaaaag cgcccatcag atggctttca atttcgccat cgccccagct tgaactaaag
    13501 cgaatggtcg cgccgctgtc gtgatagttc acttcggtcg ctttcaacca gcgtccgtga
    13561 cagttcggat tgatatgatc ttccattgat accgcaaccg cgtccggcag ttttgccagc
    13621 cagcggcggc ccacttcatc gtcggcgtta ataatcgcct gaccgcaatg atgttcagaa
    13681 taaagcagcc atttcgcagc ttcgtagtgt tccatatcac catgataatc aaggtgatcg
    13741 cggcttaagt tggtaaagac cgacgccgga aatttcaatg ccgccacacg gtgctgtacc
    13801 agcccgtggg aggaaacttc cattgcgcaa aaagtcgcgc cctgatccac cagccccgcc
    13861 agctcatgct gaacatcaac tgccgaaccg gtagtatttt ctgtcgggat cactttcccc
    13921 agcagaccgt taccaacggt gcccattacc gcgctggttt cgccaagcag ttggctccac
    13981 tgcgccagaa gctgggtcgt cgtggttttg ccgttggtgc ccgttacgcc tacgagacgt
    14041 aaattgtcag agggttcatg gtaaaagcgg cccgccagtg cagataaacg ctcgttgagc
    14101 tggctgagat agatgaccgg tacgccgtgc atttcacgga tttcaccgtc ggtcgcctca
    14161 tctttcgcct ctgcaataat ggcagccaca ccttgcgcta tcgcctgcgg gatatatcga
    14221 cgcccgtccg cctgatgacc tactacagct acaaagagat cgcccgccgc agccacacgg
    14281 ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt ctggcaccca cggagcaaga
    14341 aggtcgcgca aattacgatc tgccacctgt cccctcgcct tgattaatca caaattcatt
    14401 tttatcgccc gttgtcagcg catccggctc gatgttcatg gtacgcaata cgccgcccat
    14461 gatggcacca aagaccggcg cggaaacggc gccgccgtag tatttacccg cctgcggatc
    14521 gttgataaca acaaccagcg cgaagcgcgg ctgactcgca ggcgcaacgc ctgcggtata
    14581 agcaatatat ttattgatgt agcgaccgtc cggcccgacc tttttcgcgg taccggtttt
    14641 aatggcgata cgatagcctt taatcgccgc cttcacgccg ccgccgcctg gtagcgccac
    14701 gctttccatc atatgcacca cggtgcgaac aatggattcc gggaagacac gttcaccggg
    14761 aaccgggggg tcaactttgg taatcgacag tgggcgataa atgccgtagc tgccgatagt
    14821 tgcgtagact cgcgctaact gtaacggtgt taccattagc ccgtagccga aagagaaggt
    14881 ggccctctct atgtcagacc accgttgttt ttgaggatat aagccactgc gttctccgac
    14941 caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag taagtatcta ctaacgctga
    15001 ggacggcatc gttaacgcca gcttggaaac accgacgtta ctcgacttct gtaatacccc
    15061 ggtcagggtt aattcgctgt agcgtgccac gtctttgatt tcgtggccgt taattcgata
    15121 aggaatggta ttgagtaccg agttttcccg caccacgcca cgttgcaacg cggtcatcac
    15181 caccatcggt ttaaccgttg agcccggttc aaacacgtcg gtgatggtac ggttacgcat
    15241 cgcctctttc ggcgtgccgc tcagattgtt agggttgtat gacgggctgt tagccatcgc
    15301 cagcacttca cctgtgttga catccaccag cacggcgcta ccagattcag ccttgttaaa
    15361 ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc aggcgttcat caatactcag
    15421 cgccaggttg tgcgctgcct ggctgtcagt agaagaaata tcttcaatta cgcgaccata
    15481 gcggtcttta cgcacaatgc gctcacccgg ctgcccggta agccatttat cgaaactctt
    15541 ctcaacgccc tcaatccctt gactatcgac gttagtaaag ccgatgaggt gagcagtcac
    15601 ttcgccggac ggatagtaac ggcgagactc ttcacgcaga tgaatccccg gcagtttcag
    15661 ttttttgatg tagtccgcca tgtcagagtt tacctgacgc gccagataaa taaagcgccc
    15721 tttcgggttg gcgttaatgc gggcagaaag ctgatccagc ggaatattga gcgcgttagc
    15781 cagcgccttc cagcggtcac cgacgctgat accgccagcg tcatgcactt ctttcgggtc
    15841 agcccaaatc gcttttaccg gcacgctcac cgctaacggg cgaccagaac ggtcagtaat
    15901 catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa cgcatgtcgc cctctttcac
    15961 cagcatatcc ggggagataa cttgtaacca cgctacgcgt ccgagcagaa aagccagcgc
    16021 caggagaata cagccgcata acaacgcaaa acgccaactg ataaagttgg catgttcttc
    16081 ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg cgtcgcgttt atccttattt
    16141 ttgcactacg atattttctt gtgacggatc aacatgctgc atttgcagct tttccgtggc
    16201 gatcctttcc acccggctat ggtcgccgag cgcattctct tcaaggatca ggttgcgcca
    16261 ttcaatgtct aaagcatctc gctccagcac cagttgttcg cgctgagcgg tcagtaaacg
    16321 ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa ataatgcaaa tgaacaggca
    16381 gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata acaccaggca atgcatggcg
    16441 ctcgtggctt cccatcgatc ctttaacttt gcttagagct tctgtcactc tgctgatcat
    16501 gcgttcgtcc tctctgcaat acgcagaact gaactacggg cacgagggtt ctcagccacc
    16561 tcttcttcgc ccggcattaa cttgcctagt gctcgcagct gacggccacc cagttttttg
    16621 agctgctctt cagtcatcgg taaccctgcc ggaacttgcg gaccgcggct gttttcacgc
    16681 ataaaacgtt tcacaatacg gtcttccagc gagtggaagc tgatgatcga aagccgccca
    16741 cccggggcca gcacgttgag cgagcttttt agcgcctgct ctatctcctc cagttcactg
    16801 tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg gatgtttaaa cttatctttc
    16861 accggcgttg cagcagccac gacttccgcc agttctttgg tgcgggtcat cggctgttcg
    16921 cggttacgct cgacaatggc gcgggcaatg cgtttggcaa aatgctcttc accataggtt
    16981 ttcaataccc aggcgatatc ggcttcttct gcggtttgta gccattcagc ggctgactgc
    17041 ccacgggttg ggtccatacg catgtccagc ggaccatcgc gcataaagga aaagccgcgt
    17101 tcagcatcat caagttgcgg tgaagagacg ccaagatcga ggagaatgcc gtcgatcttg
    17161 ccaataagat cgcgctcggc aacgtattcg cccagcgcgg agaaaggtcc gtggatgatg
    17221 gagaagcgcg gatcatcaat agtcttcgca acggcgatag cctgcgggtc gcgatcgatc
    17281 gccagcaaac gcccctcttc gccaagctgc gagaggatca gacgtgagtg accaccgcga
    17341 ccaaaagtcc catcaatgta gatgccatca ggacggatat tgaggccatt aacggcttca
    17401 tccagcagca ccgtagtatg tttatagttt tccatcattt tatagagaca agtcctgcag
    17461 tcgctccgat aagtctccgg tagccaactg ctctgcgtcg atatcttcct tgacctgttg
    17521 atgccaggtt gtttcatccc acagctcaaa cttgttgaac tgtccaacca gcatcacttc
    17581 ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt aacaatcgac ctgcgccatc
    17641 catctgacat tcgctggcat gacctaacag taggcgctgc acacggcgct caaccgggtt
    17701 catgctcaac agacgcgata atttttgctc gataatttcc cattcaggca gggggtaaag
    17761 cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt tgaccggcag cgttctcaag
    17821 cagctgttcc cgataacggg taggcactga taagcgccct ttgctgtcga gattgactaa
    17881 cgttgctccc cggaacatgc cagcctcacc ccttattccc actttgcccc acaatttccc
    17941 actgaaagga gtttacggag cggaggaaaa gcttgtcaag ccagtcacaa ggcataccga
    18001 ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt tattttacgt ccaacgtgcg
    18061 aagcaaatta acgttatgaa tagctgtaag aaaaaatccc tcatcgtcat cactgtttta
    18121 aaatgactca ctattttcca cagcaaatat aaagtgtcag tttgcgacgc gagcggcatt
    18181 ttaggacata tcttccccgg ttaacagtcc ttgttacgtc tgtgtggcgc ttgatgccag
    18241 cgggtttgcg caaaagcaaa gaaaagtgtt tgttaaatcg gcaattcatc gccgcatgta
    18301 acaaaataat gcaaaaacgc gctgtcgata tcacttaatt attgtgaatt tcagctggtg
    18361 agttaattaa cgggcgggtt taaaaccatt tatcaataat agtcaggaat agtcttatt
//
LOCUS       DAJTYL010000083        18328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYL010000083 DAJTYL010000000
VERSION     DAJTYL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.083"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(139..3654)
                     /locus_tag="OO572_002734"
     CDS             complement(139..3654)
                     /locus_tag="OO572_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6661679.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO572_002735"
     CDS             complement(4107..5369)
                     /locus_tag="OO572_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6661680.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO572_002736"
     tRNA            complement(5567..5642)
                     /locus_tag="OO572_002736"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO572_002737"
     CDS             complement(5748..6455)
                     /locus_tag="OO572_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6661681.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO572_002738"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO572_002738"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6661682.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO572_002739"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO572_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6661683.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO572_002740"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO572_002740"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6661684.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO572_002741"
     CDS             complement(11639..11914)
                     /locus_tag="OO572_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6661685.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO572_002742"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO572_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6661686.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO572_002743"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO572_002743"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6661687.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO572_002744"
     CDS             13874..14200
                     /locus_tag="OO572_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6661688.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO572_002745"
     CDS             14384..15103
                     /locus_tag="OO572_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6661689.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO572_002746"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO572_002746"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6661690.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO572_002747"
     CDS             16442..17404
                     /locus_tag="OO572_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6661691.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18328)
                     /locus_tag="OO572_002748"
     CDS             complement(17505..>18328)
                     /locus_tag="OO572_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR6661692.1"
                     /translation="LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGY
                     PLSERATFAQACKAFLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIY
                     REVKWRHLPKLILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLI
                     ILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTP
                     PVGSALFVGCSISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4740 a         4470 c         4531 g         4587 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaaca
//
LOCUS       DAJTYL010000084        17834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYL010000084 DAJTYL010000000
VERSION     DAJTYL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.084"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(159..1940)
                     /locus_tag="OO572_002749"
     CDS             complement(159..1940)
                     /locus_tag="OO572_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6661693.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(1933..3705)
                     /locus_tag="OO572_002750"
     CDS             complement(1933..3705)
                     /locus_tag="OO572_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6661694.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(3735..4193)
                     /gene="decR"
                     /locus_tag="OO572_002751"
     CDS             complement(3735..4193)
                     /gene="decR"
                     /locus_tag="OO572_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6661695.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(4347..5165)
                     /gene="cof"
                     /locus_tag="OO572_002752"
     CDS             complement(4347..5165)
                     /gene="cof"
                     /locus_tag="OO572_002752"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6661696.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            5265..6957
                     /locus_tag="OO572_002753"
                     /pseudo
     CDS             5265..6957
                     /locus_tag="OO572_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            7022..7717
                     /gene="queC"
                     /locus_tag="OO572_002754"
     CDS             7022..7717
                     /gene="queC"
                     /locus_tag="OO572_002754"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6661697.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(7769..8167)
                     /gene="fadM"
                     /locus_tag="OO572_002755"
     CDS             complement(7769..8167)
                     /gene="fadM"
                     /locus_tag="OO572_002755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6661698.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(8261..8632)
                     /locus_tag="OO572_002756"
     CDS             complement(8261..8632)
                     /locus_tag="OO572_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6661699.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(8783..10654)
                     /gene="ppiD"
                     /locus_tag="OO572_002757"
     CDS             complement(8783..10654)
                     /gene="ppiD"
                     /locus_tag="OO572_002757"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6661700.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(10846..11118)
                     /gene="hupB"
                     /locus_tag="OO572_002758"
     CDS             complement(10846..11118)
                     /gene="hupB"
                     /locus_tag="OO572_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6661701.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(11327..13681)
                     /gene="lon"
                     /locus_tag="OO572_002759"
     CDS             complement(11327..13681)
                     /gene="lon"
                     /locus_tag="OO572_002759"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6661702.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(13869..15143)
                     /gene="clpX"
                     /locus_tag="OO572_002760"
     CDS             complement(13869..15143)
                     /gene="clpX"
                     /locus_tag="OO572_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6661703.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(15269..15892)
                     /gene="clpP"
                     /locus_tag="OO572_002761"
     CDS             complement(15269..15892)
                     /gene="clpP"
                     /locus_tag="OO572_002761"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6661704.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(16139..17437)
                     /gene="tig"
                     /locus_tag="OO572_002762"
     CDS             complement(16139..17437)
                     /gene="tig"
                     /locus_tag="OO572_002762"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6661705.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
BASE COUNT         4203 a         4920 c         4288 g         4423 t
ORIGIN      
        1 ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga
       61 gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca gtgcacagtg
      121 cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa tgattcctct
      181 tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat ctgccagtag
      241 cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg cccacgatga
      301 agcaccagaa tggtgtcggc atcaataata gttgataagc ggtgagcaat caccaccagc
      361 gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt accggagtca
      421 atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag cacgcgcgcc
      481 agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc cagcggcgta
      541 taaatacaat cactcatgct gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc
      601 cagacgcgtt cttcggagat atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc
      661 agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct atgacttagc
      721 gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta atagcccatc
      781 aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc cacaaagtta
      841 cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg ataagcaaat
      901 gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt gccatattgc
      961 tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc ctgttgcagc
     1021 atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag atagctgata
     1081 aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat
     1141 aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg
     1201 aggcgcaggg tttgcatcct agccatatag tgtgaacgac tggcctcccc catacgttcg
     1261 ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat gatttcgtta
     1321 aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa
     1381 cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc caccagcgcc
     1441 attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc
     1501 aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc attagtgacg
     1561 cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg gcgtaacgca
     1621 gcatccatca cgtcggtacg caactgttgc actgcgccta ctgccgcccg attaaacagc
     1681 agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata cgccgcagcc
     1741 agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc gataaaatag
     1801 ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat caggaccgca
     1861 atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag agtcggccac
     1921 agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc
     1981 cgcctccagt tgttgatagc gatacatatc gcgataccaa ccgctttgtt gcgccagctc
     2041 atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt cactggcttc
     2101 cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc cccactgacg
     2161 caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg catcatcaag
     2221 gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct gtttttgccc
     2281 accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa
     2341 aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct gggtggcatt
     2401 cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct
     2461 gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag gaatatcatg
     2521 aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca acagggtact
     2581 tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca gggcgaaatt
     2641 gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa tatttacatc
     2701 cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc
     2761 cagcatggcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt taaacatcca
     2821 tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac tggtgagctg
     2881 gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt
     2941 cgccatacca atcgcgatat agatggtcgg gtcgaagcga gcatcaatac gcgccacccg
     3001 catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact ggcgatcttc
     3061 cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc ggtcattaag
     3121 actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt
     3181 aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact gccagctaat
     3241 ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca ccagcgtcag
     3301 caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac gagccatgag
     3361 atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct gacggtaata
     3421 atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata cgcgccagac
     3481 ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc acatcaggct
     3541 cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa ccacttttgg
     3601 cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa gatagcgacg
     3661 ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt gtgtcctgac
     3721 ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg
     3781 aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt ataaaactcg
     3841 tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc accagccatg
     3901 cgccagaccc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg gcaataccat
     3961 tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag gcctattttt
     4021 tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag ccgtttcagg
     4081 cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg caaagagagg
     4141 gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa catagccctt
     4201 ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg acaatttatt
     4261 ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca
     4321 attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg tggatagtcc
     4381 agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat caccggtaaa
     4441 tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc gctaccgacg
     4501 ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat
     4561 aaacctaaat gttgggtccg caccgtcaat gcagcgcctt tattgcagcc caccggcagc
     4621 acttcgaggc aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat
     4681 agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat cttggtgacg
     4741 ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct atagacaaat
     4801 acctgcaaca acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc
     4861 atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa
     4921 tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt gccggtaatc
     4981 aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc atgacgcccc
     5041 gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc
     5101 tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc taccagacga
     5161 gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac ctgatatata
     5221 cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga ttgctcaacc
     5281 gtcttaacca gtatcaacgt ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa
     5341 ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg tcaggcacag
     5401 gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc
     5461 tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact ggaaaccgga
     5521 aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg cactctgtta
     5581 cagccattta tgggcggaca atggcaaaac gatacaccca cgttgcgtat tccctactat
     5641 cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca gcatctcgcc
     5701 gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc gattggcgat
     5761 ttagcgcatc actgggaaac ctctactgac aggttacgct gggactttta tcttcgttca
     5821 accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca gcgattattg
     5881 atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg tattgaagtc
     5941 acccatccgc agtgtctgac cttcttttta catcgccccg attactggct tgcgcaccgg
     6001 ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg cactggtcct
     6061 tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga ttattaccat
     6121 ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct tttcgaaaaa
     6181 gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc ggaggagctg
     6241 caacgggtca gccaagtcag tagtggcatc agtttaggtt tttgctattt aacgttgcgc
     6301 aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat tcatcaatcc
     6361 ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca tgcattactg
     6421 ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc gaaaaccttg
     6481 acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct acaggcgaca
     6541 ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaaa ctgggacgac
     6601 acgaccctac tggcacacgc agacctcatg atgggcgaca gattaattgg cgaagcaccg
     6661 gaatatactc tggagcaatg gctacgttgc gatccactat ggccacatgt tttcgacgct
     6721 ccagcatatg cacatctgca atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa
     6781 aaccgattta atgccctgaa agcggttttt agccagttaa tggcagacgc gacgctgacg
     6841 ccgctgttca actatcacta tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg
     6901 acaccgcgcg gctggtttga atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata
     6961 gctggtccgg gttaatcgca ggcgttacca taacgctttt attgttcaag caggattatc
     7021 tatgaaacgt gctgtcgttg tgttcagtgg aggccaggat tccaccacct gtctggtgca
     7081 ggcattacaa caatatgatg aagtccattg cgtgacgttc gattacggtc agcgacatcg
     7141 cgcagaaatc gacgtggcac gcgaactggc gctgaaactg ggagcacgcg cgcataaggt
     7201 tcttgatgta acgctgctca acgagctggc ggtcagcagc ctgacacgtg acagcattcc
     7261 ggtgcctgat tatgaacctg aagccgatgg catcccgaat acgtttgtcc cagggcgtaa
     7321 tattttgttc ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag aagccgtaat
     7381 taccggcgtc tgtgaaacgg atttctccgg ctacccggat tgccgcgatg agtttgtgaa
     7441 agcactaaac catgccgtca gtttgggcat ggcgaaagat attcgttttg aaacgccgct
     7501 gatgtggatt gataaagcgg aaacctgggc gctggcagat tattacggca aactggattt
     7561 agtccgtaac gaaacgttga cctgctataa cggcattaaa ggcgacggtt gcggtcattg
     7621 tgcggcatgt aatttacgcg ccaacggttt gaatcattat ctggccgata aaccgacggt
     7681 gatggcagcg atgaagcaga aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc
     7741 ggcttgagca tccggcacca caaaacgttt acttaaccat ctgctccagc ttttcgcgca
     7801 attccccttc cagagctaat gctttctgcg ttttaagatc aatacaaaca aacgtaataa
     7861 gcgcatccgc taccacctgc ccttccggct ccagtgtaat gacctggttt aagatgccgc
     7921 ttttaccgtt taattgctgc aactgactgg taatagttaa caggtcgctt aataccgctg
     7981 gacgacgata gttaatattg atattgacca cgacgaaggc gatgttatgg gccgtcatcc
     8041 actgaaaact gtcgctattt tccaccccat cccagcgggc ttcttcgaga aattcaaggt
     8101 agcgggcgtt gttgacgtgc tggtaaacgt cgagatgata tccacgaact ttgatttgtg
     8161 tttgcatagc gcaataacct tacgttgttg ttataagtac agaggtcata actggtatga
     8221 cctcttcagt ctggcaaaaa attgccacta tgcaaattaa ttacagggtt aataccgcca
     8281 gattacgttc caccagcgaa ttgcccatcc ccggcacctg ctttagatcc tccaccgttt
     8341 taaacggacc gtactcttcg cgataactga caatcgcctg cgctttcttc aggccaacgc
     8401 cattcatcgc gcgggctagc tcttccgcgc tggcattatt aatgctgacc cgggtgcctt
     8461 cttcgtcact ggctttcgcc ggtactgctg ctttactttg tgctgcagga gcttccgctt
     8521 tggtttctac cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta tgagacattc
     8581 cggcacaggc cagggacagt gtaatgagca gtgctttaat tccgtgtttc atactgtttt
     8641 ctccttgttt gttaacagtg gaatcacctt aacgggagtg aaaacagtaa acaaatggca
     8701 atgttcagaa atggaaaagg ccgcgaaagc gaccttttct tgttacagct gcaacgcatt
     8761 tttttgcgag gcggcttcag gattattgct gttccagcgc atcgccaatt ttgattttcg
     8821 cctctttacg caggttactc atcagagctt caaagacgat ttgtgcgttg ttctgggtga
     8881 taccctgcac catcgctttt ttctgatctt ccggcattga accttgtttc acttcatcca
     8941 gcgccagcag aaccacatta ccttgcatat cggtcgccat accgtagctc ggtttgtctt
     9001 tcgctggcag tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg gaacggctta
     9061 aggttttcgg ctcgccaaat ttcagaccgg cagcctgcat agcttccgca cctttgccgg
     9121 ctttcaaatc aaccagcagt ttctcagcat ccactttcgc ctgttgttca gctttgttgt
     9181 gctgaaccag tgccttaact tgttcctgaa catctgccaa cggtttcacc gcttccggtt
     9241 tgtgctcgct gacgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt
     9301 tgatgcccga cgcgccgttt tcacctacca gaccgccgtt aaagatagcg tcggcaaccg
     9361 gcttgaagtt caactcttcc ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt
     9421 taacgccggc agcttgctct gcaccggcca gagactcggt gtcgttgctt gccgcatcgc
     9481 tcactttctg ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc actttcgccg
     9541 caatgtcgtc acgtacttcg tctaacgatt tcactttcgc tggctgaatg tcgtccagac
     9601 gtacaatcag gaaaccgacc gaagatttga tgacaccaga cagttggcct ttttctttca
     9661 gaccagcatt tttcagttcg tcggggatag tggcatcttc taaccaaccc atatcgccgc
     9721 cgttacgggc agagataata tcggcagatt tttctttggc taatgcagca aaatcaccgc
     9781 ctttattcag ctcatcaagt accgctttcg cttcatcttc agttttggtc tggatgatgc
     9841 tgtagcgggt acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag tagctctgga
     9901 tatccgcatc gctaaccggt tgctgcatcg ttgcggcaac cagcttgatg taactcacgc
     9961 ggaattgttc cggcgtcatg aaattgtttt tgttttgttc gtagtagctg gcaatctcct
    10021 gttcggtcac aggctgcttc gccgccagcg cgttaacatc gatagtcgcc tcacgcacca
    10081 cgcgttgttg cgcgaccagt gccgccagct cgtcggtttc acctttcagc ataaaatcgg
    10141 taccggcaac gccgttaatc agctgttggg tagtgagctg gttacgcagc gcctgggcgt
    10201 actgatcggc ggtcatcccc atctggttga ggataccgtt atagcggctg ttatcaaatt
    10261 tgccgtcaac ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc tcatcactga
    10321 tacccagttt cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc agacgattca
    10381 gcacctgttg acgcagggtt ttcatatagc cttcgttcgc tgccagctcg gagtattgat
    10441 cgcccagctg ttgctgcata cgattacgct cgctgttaaa ggcgttctcg aactgcccac
    10501 ggctgatttc ctggtcattc acttttgcgg cgtagttatt gcctccgcca atcaggtaac
    10561 cactcacgcc ggtcaatatg aacgacacga taatgatacc gaaaataatc ttgagcacga
    10621 gactgtttgc agccgtgcgt aagctgtcca tcatggtgta acaacactcc gctgtagatg
    10681 actgtgtacc tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg
    10741 gcaattgtca gcccacaaga cacagaaagt caccgaatac aaataaaaaa ggcacatcag
    10801 tagatgcgcc cttgaacttc gtcacatccc cactggggac aacgcttagt ttaccgcgtc
    10861 tttcagtgct ttacctgcac ggaagctcgg tactttagcg gcagcgatgg tgatctcttt
    10921 accggtctgc gggttgcggc cagtacgggc agcacgctct ttaacggcaa aagtaccaaa
    10981 acctaccagt gctacatcat ccccttcttt cagagattca gttacggaag caataatagc
    11041 atctaacgca cggccagccg cagctttaga gatatcagcc cctgcagcaa tcttgtcgat
    11101 caattgagat ttattcactc ttctcttcct ctttataatt tatatcgcac ttgaatcctt
    11161 caaggtacga acgcgcagca gttatatcag gccagccatc cccttacaac accccttaat
    11221 aagggcaagc ccgatccgct atctaactta gcgagacaaa aaaaggctgg caagcccgaa
    11281 ttagcctgcc agccctgttt ttattagtgc attttgcgcg aggtcactat tttgcagtca
    11341 caacctgcat gccagacggt tcattttgca gcgccagagt cagaacttcc tcaatgcgct
    11401 tcacaggatg aatgtccaga tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt
    11461 tattttcgaa cggaattagc actgttttaa tcccgccgcg atgcgctgcc aggagttttt
    11521 ctttcaaacc accgatcggc agtacctgac cacgcagagt gatctcaccg gtcattgcca
    11581 catcggcacg aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac atagcaatac
    11641 cggcactcgg accatctttc ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt
    11701 tttcgtaaaa atcagggttg atccccagtt tttccgcacg cgcacgaacc accgttaacg
    11761 ccgcctgaat agactcctgc atcacttcgc cgagcgaacc ggtataggtc agtttgcctt
    11821 tacccggaac acatgcggtt tcaatggtca gcaagtcacc gcccacttcc gtccacgcca
    11881 gaccgattac ctgaccgaca cggttttcgt tatcagcgcg accatagtcg aaacgctgaa
    11941 caccgaggta gtcatgcagg ttatcgccgt taatttcgat atgttttaat gacttatcga
    12001 gcagtaactg cttaaccgct ttgcggcaca gtttggagat ttcacgctcc agaccacgca
    12061 cgcccgcctc acgggtgtag taacgaataa tgccgataat ggcgctatcg tcgacggtca
    12121 gctcaccttt tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga
    12181 tgttcagttt ttcatcttcg gtataaccgg agaggcgaat cacttccata cgatccagca
    12241 gcggtgccgg aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt
    12301 aatccacttc caggtagtgg tcgctgaacg ctacgttctg ctctggatcc agcacttcaa
    12361 gcagtgcaga ggccggatcg cctcgcatgt cagaagacat tttgtcgatc tcatcgagca
    12421 ggaacagcgg atttttcacg cccactttcg ccattttctg gatcagttta cccggcatag
    12481 aaccgatgta agtacggcgg tgaccacgga tttccgcttc atcacgcacg ccgcccagcg
    12541 ccatacggac atatttacgc ccggtggctt tggcaatgga ctgaccaaga gaggttttac
    12601 ctacccccgg cggccctacc aggcagagga tcggtccctt gattttgttg acacggcttt
    12661 gaaccgcaag gtactcaagg atgcgatctt tcacgcgctc cagaccataa tggtcggtat
    12721 caaggatttc ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca ttccacggta
    12781 cctgtaccat ccagtcgata taaccacgca ctacggtcgc ttctgccgac atcggagaca
    12841 tcattttcag cttctgcaac tctgcttccg ctttctcttt tgcctctttc ggcatcttcg
    12901 ccgcgtcgat tttgcgcttc agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga
    12961 gttctttctg aatagctttc atttgctcgt tcagatagta ctcacgctgg gatttctcca
    13021 tctgcttttt aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg atttccgatt
    13081 ccatcattgc catcagatat tccagacgtt cgttaacgtc ggacatctcc agaacggact
    13141 gtttgtcagc cagtttcagc ggcatatgtg cagcaatggt atccgccaga cgcgccggat
    13201 cgtcgatgct attcagcgac gtcagcactt ctggtgggat ttttttgttc agcttgatgt
    13261 agccttcgaa ctggctgatt gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg
    13321 tcggcgactc cagatactcc gccttcgcag aaaagtgttc gccattgtca gagagcgcag
    13381 aaatacgcgc gcgctgtaac ccctcgacca gcactttgac ggtgccgtca ggcagtttca
    13441 gcatctgcaa tatagaggcc acggtcccga cggtgaaaag atcgtttaca cccggctcat
    13501 ccgttgaagc ttctttctgc gcgaccagca taattttttt atcatggtcc atcgccgctt
    13561 ccagacaacg gatagatttt tcccgcccga caaataaggg gatgaccatg tgcggataaa
    13621 ccaccacatc gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc tcaggattca
    13681 tagagctctc tcttagttta atttccgcca ggtaatcaga tgacacgact gtgcttcacg
    13741 ccatctatta acatgtacgt cagtatatgg ggatgtttcc cccacattca acgccgagaa
    13801 tagaggaaaa attaaagggg agataaaatc cccccttttt ggttaactaa ttgtatggga
    13861 atggttaatt attcaccaga tgcctgttgc gcttccggct taccataaat cagcaacggt
    13921 ttgctttgac catcaattac cgactcgtcg ataaccactt tttcgacgtc ttccatggac
    13981 ggcagatcgt acatggtatc gagcagtgcg gcttctacga tggaacgcag gccacgggca
    14041 ccggttttac gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc acggaattcc
    14101 agatccacgc cttccagatt aaacagcgcc tgatactgct tggtcagggc gtttttcggc
    14161 tctttgagga tctgaatcag agcttcttcg ctcagttcat tcaacgttgc tacaaccggc
    14221 agacgaccaa tgaactcagg gataagacca aacttgatca gatcttccgg ttcaacctgc
    14281 gccagcagct cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca
    14341 atgccggagc cggtttctac acggtgggaa atcactttat ccagaccggc aaacgcaccg
    14401 ccacaaataa acaggatctt agaggtatca acctgcaaga attcctgctg cggatgttta
    14461 cgcccacctt gcggtggaac agcagctacc gtaccttcga tcagtttcaa cagtgcctgc
    14521 tgtactcctt caccggaaac gtctcgggta atggacgggt tgtctgactt acgagaaatc
    14581 ttgtcgattt catcgatgta gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac
    14641 ttttgcaaca gcttctgaat gatgttttca acgtcttcgc ccacataacc ggcttcggtc
    14701 agcgtggttg cgtcggccat ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc
    14761 agcagcgttt taccggaacc ggtcggaccg atcagcagaa tgttactttt gcccaactcg
    14821 acaccattgc tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc
    14881 agcacttttt tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt gcgaatttca
    14941 tgcggcgtcg gtagcgcact gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga
    15001 atgatgtcgt tacataaatc aacacattcg tcgcagatat acacggatgg accggcaatc
    15061 agcttgcgca cttcatgctg gcttttgccg caaaaagagc aatacagcaa tttgcctgag
    15121 ccatctttgc gtttatctgt catgggtcaa aacctcttct ttgttctttg tgccgcacac
    15181 gacgacgcaa atgccattct caggcgcaag ccgcttacag cgttgtgccg ccctagataa
    15241 gtatagcggc acagttgcgc ctctggcatc aattacgatg ggtcagaatc gaatcgacca
    15301 gaccgtattc caccgcttca ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa
    15361 tctgttctaa tgattgaccc gtatgaagcg ccataagttc attcatgcgc cctttaactt
    15421 tcagaatttc acgggcatga atttcgatat cggtcgcctg gccctggtag ccgcccaacg
    15481 gttggtgaat catcacgcgc gaattcggca ggcaaaaacg tttacctttt gcccctgcgg
    15541 tcaacaagaa agcgcccatc gaggccgcct ggcccataca gatggtgctg acatcaggct
    15601 tgataaactg catggtgtca tagatagaca tcccggcagt gatcaccccg cctggggagt
    15661 taatgtacag atagatatct ttttctgggt tttccgcttc caggaacagc atctgcgcca
    15721 caatcaggtt agccatgtgg tcttcaacct ggccagtcag aaaaatgacg cgttccttaa
    15781 gtagacgaga atagatatca aaagagcgct caccgcgtga ggtctgttca atgaccatcg
    15841 gcaccagcgc catatggggt gcaaagttat ctcgttcgcc gctgtatgac atttccgtct
    15901 cctggataaa attgaaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag
    15961 ctagtccctg tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt
    16021 tacgctaaca ccgaaagagg cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa
    16081 gcccaccacc tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt
    16141 acgcctgctg gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg
    16201 ccagtacagc ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt
    16261 ctttgttttt gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct
    16321 cttcgatcag gcctttcacg cgctcttcgt cagctttcag ctcgttggtg cggataactt
    16381 cgcccagcag caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg
    16441 gcagttccag agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa
    16501 cgtcgatttc gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac
    16561 cttcgatcgc ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt
    16621 ttttacgcac ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga
    16681 tgaattctgc agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga
    16741 atttcgctgc tttacctttc aggttttctg cgtggtattc ttccgggaag gtcacgtcga
    16801 tggtgaactc ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac
    16861 gaccctggcc catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt
    16921 ctacagaacc ggtgaagtcg atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt
    16981 ctttccaggt cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg
    17041 tcacttcaac gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg
    17101 gataaacttc aaactctaca gagtaagtga agtcttcacc cagcttgtat tcgcccggaa
    17161 cataagtcgg tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac
    17221 ggctcatcag gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga
    17281 tattcattgg cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga
    17341 ccagctcgct tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc
    17401 ggccaaggcc ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag
    17461 tgctcggtca actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc
    17521 ttataatcaa tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg
    17581 gcattatagc ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt
    17641 tttcacaatt cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca
    17701 aaacaaaacg gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc
    17761 cgacagttgc aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca
    17821 aagacggtgt cctg
//
LOCUS       DAJTYL010000085        17345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYL010000085 DAJTYL010000000
VERSION     DAJTYL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.085"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..343
                     /locus_tag="OO572_002763"
     CDS             <1..343
                     /locus_tag="OO572_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6661706.1"
                     /translation="FEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITG
                     NDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDV
                     LSSLVMRVAQD"
     gene            424..1098
                     /locus_tag="OO572_002764"
     CDS             424..1098
                     /locus_tag="OO572_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6661707.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1114..3576
                     /locus_tag="OO572_002765"
     CDS             1114..3576
                     /locus_tag="OO572_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6661708.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGATVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3592..4625
                     /locus_tag="OO572_002766"
                     /pseudo
     CDS             3592..4625
                     /locus_tag="OO572_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4707..5045)
                     /gene="rcnB"
                     /locus_tag="OO572_002767"
     CDS             complement(4707..5045)
                     /gene="rcnB"
                     /locus_tag="OO572_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6661709.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5264..6094)
                     /gene="rcnA"
                     /locus_tag="OO572_002768"
                     /pseudo
     CDS             complement(5264..6094)
                     /gene="rcnA"
                     /locus_tag="OO572_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6216..6488
                     /gene="rcnR"
                     /locus_tag="OO572_002769"
     CDS             6216..6488
                     /gene="rcnR"
                     /locus_tag="OO572_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6661710.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6580..6676
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6711..7499
                     /gene="thiM"
                     /locus_tag="OO572_002770"
     CDS             6711..7499
                     /gene="thiM"
                     /locus_tag="OO572_002770"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6661711.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7496..8296
                     /gene="thiD"
                     /locus_tag="OO572_002771"
     CDS             7496..8296
                     /gene="thiD"
                     /locus_tag="OO572_002771"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6661712.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8361..9182
                     /locus_tag="OO572_002772"
     CDS             8361..9182
                     /locus_tag="OO572_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6661713.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9230..9976
                     /locus_tag="OO572_002773"
     CDS             9230..9976
                     /locus_tag="OO572_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6661714.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9950..10915)
                     /locus_tag="OO572_002774"
     CDS             complement(9950..10915)
                     /locus_tag="OO572_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6661715.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10912..11915)
                     /locus_tag="OO572_002775"
                     /pseudo
     CDS             complement(10912..11915)
                     /locus_tag="OO572_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            11955..13007
                     /gene="fbaB"
                     /locus_tag="OO572_002776"
     CDS             11955..13007
                     /gene="fbaB"
                     /locus_tag="OO572_002776"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6661716.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13315..14169
                     /gene="gatY"
                     /locus_tag="OO572_002777"
     CDS             13315..14169
                     /gene="gatY"
                     /locus_tag="OO572_002777"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6661717.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14198..15460
                     /gene="gatZ"
                     /locus_tag="OO572_002778"
     CDS             14198..15460
                     /gene="gatZ"
                     /locus_tag="OO572_002778"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6661718.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15470..15922
                     /gene="gatA"
                     /locus_tag="OO572_002779"
     CDS             15470..15922
                     /gene="gatA"
                     /locus_tag="OO572_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6661719.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15953..16237
                     /gene="gatB"
                     /locus_tag="OO572_002780"
     CDS             15953..16237
                     /gene="gatB"
                     /locus_tag="OO572_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6661720.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16241..>17345
                     /locus_tag="OO572_002781"
     CDS             16241..>17345
                     /locus_tag="OO572_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6661721.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITL"
BASE COUNT         4517 a         3985 c         4430 g         4413 t
ORIGIN      
        1 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
       61 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
      121 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
      181 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
      241 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
      301 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
      361 ttctccaggt aataaacatg gccgctatcc ccatggcggc tttttaattt aagaggcatt
      421 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
      481 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
      541 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
      601 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
      661 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
      721 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
      781 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
      841 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
      901 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
      961 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     1021 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     1081 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     1141 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     1201 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     1261 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     1321 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     1381 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     1441 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     1501 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     1561 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     1621 tcgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     1681 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     1741 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     1801 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     1861 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     1921 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     1981 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     2041 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     2101 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggggc gagcaagcaa
     2161 agtgattttg tccaggcagg ttatcagtat ggttttaata acttattgac gctgtatggc
     2221 ggcacgatgg ttgctaataa ttactatgcg tttaccctcg gaacgggttg gaacacgcgc
     2281 attggcgcaa tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg
     2341 tttgacgggc aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt
     2401 tttggtctgg cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt
     2461 tgggcaaaca ataaagataa ttatcgccgt gatgaaaacg atatctatga cattgccgat
     2521 tattaccaga acgattttgg ccgtaaaaat agcttttctg ccaatatgag ccagtcattg
     2581 ccagaaggct ggggttctgt gtccttaagt acgttatggc gagattactg ggggcgtagc
     2641 ggcagcagta aggattatca gttgagttat tccaataact ggcggcggat aagctatacc
     2701 ctcgcggcaa gccaggcgta tggcgagaat catcatgaag agaaacgttt taatattttt
     2761 atatcaattc cctgtgattg gggtgatgac gttacgacgc ctcgtcggca aatatatatg
     2821 tctaactcaa cgacgtttga tgatcagggt tttgcctcaa ataatacggg attatcagga
     2881 acagtaggga gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga
     2941 aatgaaacga cagctggggc gaatttgacc tggaacgcgc cggttgcgac agtgaatggc
     3001 agttatagtc agtcgagtac ttatcgacag actggagcca ctgtttcagg gggcattgtc
     3061 gcctggtcgg gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat
     3121 gcgccaggaa ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat
     3181 ggagtggtgg tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg
     3241 tcgcaaagcg atagcgaagc agaattacgt ggcaaccgga aaattgccgc cccttatcgc
     3301 ggcgcggttg tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg
     3361 ttaagagcgg atggtcaacc attaacgttt ggttatgaag tcaatgatat ccatggtcat
     3421 aatattggcg ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca
     3481 tcggttaatg tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa
     3541 gagattgatg aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt
     3601 cgcataatgc tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat
     3661 agtgagttat ctgttcagca caatttggtt gttcagggga attttgcact tactcaaaca
     3721 caaatagcga catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc
     3781 acccctatga gcccatcgga tattattgtt ggactttata acgataccat taaattaaat
     3841 ttacattttg aatggaccaa taaaaacaac atcacgttgt caaataatca gagcagtttc
     3901 accagtggtt attcggtaac ggtgacacct gcggccagta atgcaaaagt gaatatttct
     3961 gcggggggcg gtggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca
     4021 tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg
     4081 ggtgcatgtg taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttc
     4141 aatctgacat tgtcatacaa cccgataacc acaacctgca aacaggacga tttattaatt
     4201 actttagaca gtattcccgt tttacaatta ccagccacag gtaacaaagc aacaataaat
     4261 agtaaaaaaa gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca
     4321 tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt taaccaacag caacacaata
     4381 ctgaaaggtg cggaagataa tgcgtaggat ttattcttga aagtaatggt tcgccagtca
     4441 cacttttaaa tatcactaac agcagtaaag gatacacaaa tttaaaagaa attgcggcaa
     4501 agtcaaaact tacagataca acggtttcaa ttccgataac agccagttac tacgtctacg
     4561 atacaaacaa agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg
     4621 actaataaca agcaaaacga gcaagtggct aataataaaa aataacaagg tgtctgcatt
     4681 tccccctcat gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct
     4741 ttaatgattt taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag
     4801 tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca
     4861 tcggtgcggt acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg
     4921 aaactactta actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt
     4981 gcggcccagg cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg
     5041 gtcataaact ctccactttg gggatctgta atatcagtcg catatctttc ctgtcataca
     5101 atataactga caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga
     5161 aaggatgccc taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga
     5221 atcatcctgg gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca
     5281 tgaagccatg tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg
     5341 gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga
     5401 ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga
     5461 ccagtgttgc gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg
     5521 ctgccgggca ggggataaag ccaccggtta agccaaataa caaaatttgc cagttgatga
     5581 cctctctacc atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct
     5641 gatactcgcc atgttcgtga tggtgatgat gtccttggtc gtggtgatgt tcgtgatcgt
     5701 gatgatggtg atgatgctca taatcatgcc cgtgcatatt ctccagccag ttgcgttcgc
     5761 cgcgctaggt acgccagaac atccagaacg cggtgccaat aatgatcact gcggaaatca
     5821 gctggagcca cggttctgct gattgagcag taaagcgctt actgatcacc atcccgccaa
     5881 aggcaatcaa ccagaccacc gcggtatgcg aaatggttgc tgccagtccg agcatcaccg
     5941 cctgtttaat ggtgcctttg atggcgatga taaacgccgc catcatcgtt tttgagtgcc
     6001 ctggttccag gccatgaagc gcaccaagta agatggcgct ggggatgaag aaccaggcgt
     6061 ttccttgctg aagaagagtt gtaaattcgg tcatgataat aattcttagt attaattcgg
     6121 caatctgatt ctactccccc ccagtacctg atactacccc cccagtagat taatagtgct
     6181 atgatttttc atattcttgt taaccaggtg ttgccatgtc tcatacaatc cgtgataaac
     6241 agaaactgaa agcgcgtgcc agtaagattc agggccaggt cgtggcgctc aagaaaatgc
     6301 tcgacgagcc gcacgaatgc gctgcagttt tacaacagat tgctgctatc cgtggagcgg
     6361 taaacggtct gatgcgggaa gtgattaaag gtcatctgac ggaacacatc gttcaccagg
     6421 gggatgagct aaaacgtgaa gaagatctgg atgtcgttct gaaggtgctg gattcatata
     6481 tcaaataatt tattaacgcg attgtaaaac tgccgttttt cctcgtttac aacgggggcg
     6541 ctggacatta ccatcctcct ctgcgattta tcatcgcaac caaacgactc ggggtgccct
     6601 tctgcgtgaa ggctgagaaa tacccgtatc acctgatctg gataatgcca gcgtagggaa
     6661 gtcacggacc accaggtcat tgcttcttca cgttatggca ggagcaaact atgcaagtcg
     6721 acctgctgag ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa cattcccctc
     6781 ttgtgcactg catgaccaat gatgtggtgc aaacctttac cgccaatacc ttgctggcgc
     6841 tcggtgcatc gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg
     6901 ccagtgcctt gttgattaac gttggcacac tgacgcagcc acgcgcacag gcgatgcgtg
     6961 ctgccgttga gcaagcaaaa agctctcaaa cacgctggac gcttgatcca gtagcggttg
     7021 gtgcgctcga ttatcgccgc catttttgtc atgaactttt atcttttaaa ccggcagcga
     7081 tacgtggtaa tgcttcggaa atcatggcat tagctggcat tgctaatggc ggacggggag
     7141 tggataccac tgacgccgca gctaacgcga tacccgctgc acaaacactg gcacgggaaa
     7201 ctggcgcaat cgtcgtggtc actggcgagg tggattatgt taccgatgga catcgtatca
     7261 ttggtattca cggtggcgat ccgttaatga ccaaagtggt aggaactggc tgtgcattat
     7321 cggcggttgt cgctgcctgc tgtgcgttac caggcgatac gctggaaaat gtcgcatctg
     7381 cctgtcactg gatgaaacaa gccggagaac gcgcagtcgc cagaagcgag gggccaggca
     7441 gttttgttcc acatttcctt gatgcgctct ggcaattgac gcaggaggtg caggcatgaa
     7501 acgaattaac gctctgacga ttgccggtac tgatccgagt ggtggtgcgg ggattcaggc
     7561 cgatcttaaa accttctcgg cacttggcgc ttatggttgc tcagttatta ctgcactggt
     7621 ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt gagcctgatt ttgtcgccgc
     7681 ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc actaaaatcg gtatgttggc
     7741 ggaaaccgat attgttgaag cggtggcaga acggttgcaa cgttatcaga tccaaaacgt
     7801 ggtactcgac accgttatgc tggcaaaaag cggcgacccg ctgctttcac cttcggcggt
     7861 tgctacgctg cgcagtcgat tattgccaca ggtttcatta ataacgccaa acttgcccga
     7921 agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc tggaacaagg
     7981 gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc atctggatga
     8041 tgagcaaagc ccggactggc tgtttacccg cgagggtgaa caacggttta ccgcaccgcg
     8101 cattatgacc aaaaacaccc acggcactgg ttgtacactc tctgcggcgt tggctgcact
     8161 acgcccgcgc catacaaact gggctgacac cgtacaggag gcaaaaatct ggctttcatc
     8221 ggcgttagcc caggccgaca cgctggaagt tggtcacggt attggtccgg ttcaccactt
     8281 ccacgcctgg tggtgagcac cgtactatag ctgctttggt tattgtcagc ctttggcact
     8341 gacattcagg gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa ctcaccgctc
     8401 tattatgcgc tcttgggcta atttccatcg ttgcaattta tccgcgtcag acggtgaatt
     8461 ttttctattc gacagcagtt cagattacag actacatcca cttctacggt tatcgcccgg
     8521 ttaaatcttt cgctattcgc attcctgcca gttacaccat tcacggatta gatgtttcgc
     8581 gctggcagga gcgtatcgac tggcagcggg tggcaaaaat gcgcgacaac gatatccgct
     8641 tacagtttgc ttttattaag gctacggaag gcgaaaagct ggtggacccc tatttttcgc
     8701 gtaactggca attaagccgc gaaaatggcc tgctgcgcgg ggcgtatcat tatttctccc
     8761 cgtcggtatc cgcttcagtt caggcgcgat tatttctgca aacggtggat ttctcacaag
     8821 gcgatttccc tgccgtgctg gacgtagaag aacggggaaa attatcggca aaagaattac
     8881 gcaagcgggt aagtcagtgg ctaaaaatgg tcgaaaaacg cacgggaaaa aagccgatta
     8941 tttactcagg agccgttttt tatcacacca atctggcggg ctatttcaat gaatatccgt
     9001 ggtgggtggc tcactattat caacgtcgtc cggacaatga cggcatggcc tggcgcttct
     9061 ggcagcattc cgaccgtgga caggtagatg gcatcaatgg tccggtggat tttaatgtgt
     9121 ttaatggcac ggggatgagt tgcaggcatt cgttgatggg attaaagaaa cgccttaaat
     9181 aagaagcagt gatatactcc caccagactc cctggcagga aaaagacaaa tggaacaagc
     9241 gcatacccag ctgattgccc aactgaacga gcgtatttta gcggcggata acacgccgct
     9301 ttatattaag tttgccgaaa cggtaaaaaa tgccgtgcgc agcggggtgc tggagcatgg
     9361 caatattttg cccggtgagc gtgatttaag tcagttaacc ggcgtttcgc gcattacggt
     9421 gcgcaaggcg atgcaggcgc tggaagaaga gggcgtggtg acgcgttcgc gcggttacgg
     9481 cacgcagatc aacaacatct tcgaatactc gttgaaagaa gcgagaggtt tttctcagca
     9541 ggtggtattg cgcgggaaaa agcccgatac gctatgggtt aataagcgtg tcgtgaaatg
     9601 tcccgaagaa gttgcgcagc agctggcggc cgaagcggga agtgatgtct ttttgcttaa
     9661 gcgtattcgc tatgtcgatg aagaagcggt atcgattgag gaatcgtggg ttccggcgca
     9721 tttaattcat gatgttgatg ccatcggaat ttctctttat gactatttcc gcagccagca
     9781 catttaccca caacgtacgc gttcccgcgt tagcgcccgg atgccggatg ccgagtttca
     9841 gtcacatatt cagttagata gcaaaatacc ggtgctggtg atcaagcaag tggcgcttga
     9901 ccaacagcaa cggcctattg agtacagcat tagccactgt cgcagcgatc tatacgtttt
     9961 tgtgtgcgag gagtagttcc tcgcgcgttg gcgcacaatc accgccccga tgcccgacaa
    10021 cccacgacgc cactgcattg cccagtaata cggcatccgc cagtggcaga ccagaggcca
    10081 gcccggcaag tacgccacct gcatgactgt cgcccgcccc aatggtgtct acaacttgcg
    10141 ttggaaatgc cggaatgcag ccagaagcgt cgttgctgaa ataccatgcg ccttctttat
    10201 cgagacgaac gatcaacggc gcggcaaatt tctcctgcca ttgcttgcca agtgttgtta
    10261 tctctgcgga taaagcaaaa cgttcggcgg caatctcagc ctcttgacga ttgagtgaca
    10321 ctaaaggtcg acaggccatg atccgcgcca gtaatgcgtc cgggatatcg ccaatacgtg
    10381 ggcaaaaatc gataaacggc gtcacgtctt gcagcttttc cagccattcc actaacaatt
    10441 cgccgcaggg cgaggccagt tgataaccgg aaaaatagag caggttgcca ggcgcaacgg
    10501 ttaatcgtgc caaccattgg cgattccatt gattttccac accgctgaat gacataaaag
    10561 tgcgttcgcc atccggctca accagtgcca gacaccagcc gttatcgctt tcggcgttat
    10621 cgatcaggct gattaacccc tctttcgcca tccggttgcg aataatctcc gcccacacgc
    10681 cctgaccgag cggcaaggca ttacccgctt cgatgccgag gcgctttaac gccacggcaa
    10741 tattcagtgc gcagccgcca acgttaacgc tctgctgttt cagttcgata tcgcacccac
    10801 gccaggggag ggcataagcg tcggcgatca cgtcaatgac cgccgcgccg acgaccatca
    10861 ccggctgacg cgtggttaat tcaggcagca gtgtgtgtaa tcgagcgccg ctcataccgc
    10921 ctcccgttgt tgacgatatt tcgccagcgc tgtggcatag cggttgaaat caagctgatt
    10981 taccgcatcc agctccgcct ttaacgcagg atcgacagcg ttaatgccat gcaacgcgcc
    11041 gcaaatcgcc gtcgccatag cacctatggt gtctgtgtcg ccgccaagat tggcgcacag
    11101 gacggcgcag cggtttgggt cggtttgtgc cagttcaact agcgcaatgg cgcacggaac
    11161 ggactcaata gtgctggtgc ctgcgccaac gacctggtaa agctgttcgc tggcggattc
    11221 ggtgccgtcg gcattacgca caattttcag cgcaatctcc agacgtgccg ccagtgaggc
    11281 gctgaacgtg gtggtgcgtt tttgctgtgc atgtcgcgca attgaaggca atgaatcaac
    11341 aatcgctgac cagcgttctc cgtcaatggc acgagaaatc gcccatgcga tgactaccgc
    11401 gcctgcaacc gccagatcgg atttatgggt cgggctggac gccagcgcca catcatcaat
    11461 aaaggaatca acatcacgcg ctggaagcaa acaacctaat ggcgagacgc gcatcgctgc
    11521 gccgttggtt acgccgttgt tttccagttc agcaacgggt ttaccgtcgc gaatggcgtt
    11581 aagcgcaatc ttcgaggtcg gacctagtac gtttttgtta aaggcgtcga aacgcaacgc
    11641 ccagtcgaga atattacgcc caatcagatt cggatcgatc ttgccttcac attccagtaa
    11701 cgcatccgcc agacacaggc catcgaggta tcgtcggtga attcggcgcg gttaaaataa
    11761 caggccgcgt tattctcctt tggtccaggc agaaaacggt caatccagcc aaagtgtgct
    11821 ttaacgcggc tgcgtggcca aagctcggag ggcatcccca tcgcatcccc taacgcctgc
    11881 ccataaagag caccgagaat acgttctgtt ttcatttaac ttccccttga atcggaattt
    11941 acgggagcat agtaatgaca gatattgcgc agttgcttgg caaagacgcc gacaaccttt
    12001 tacagcaccg ttgtatgact attccttctg accagcttta tctccccgga catgactacg
    12061 tagaccgcgt gatgattgac aataatcgcc cgccagcggt gttacgtaat atgcagacgt
    12121 tgtacaacac cgggcgtctg gctggcacag gatatctttc tattctgccg gttgaccagg
    12181 gcgttgagca ctctgccgga gcttcatttg ctgctaaccc gctctacttt gacccgaaaa
    12241 acattgttga actggcgatc gaagcgggct gtaactgtgt ggcatcaact tacggcgtgt
    12301 tggtgtcggt atcgcggcgc tatgcgcatc gcattccatt tctcgtcaaa cttaatcaca
    12361 acgagacgct aagttacccg aatacctacg atcaaacgct gtatgccagc gtggagcagg
    12421 ccttcaacat gggcgcggtg gcggttggtg cgactatcta ttttggctcg gaagagtcac
    12481 gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc gcacgagctg ggcatggtga
    12541 cagtgctgtg ggcctatttg cgaaactccg cctttaagaa agatggcgtt gattaccatg
    12601 tttccgccga cctgactggt caggcgaacc atctggcggc aaccataggt gcagatatcg
    12661 tcaaacaaaa aatggcggaa aataacggcg gctataaagc gattaattac ggttataccg
    12721 acgatcgcgt gtacagcaag ttaaccagcg aaaacccgat tgatctggtg cgttatcagt
    12781 tagctaactg ctatatgggc cgggctgggt tgataaactc cggcggtgct gcaggcggtg
    12841 aaactgacct cagcgatgca gtgcgtactg cggttatcaa caaacgcgca ggcggaatgg
    12901 ggctgattct tggacgtaag gcgttcaaga aatcgatggc tgacggcgtg aaactgatta
    12961 atgccgtgca ggacgtttat ctcgatagca aaattactat tgcctgatac gcctcatacg
    13021 aacagcacca ccccgtagga cggataagac gtttacactg catccggcaa cctatgcctg
    13081 atgcgacgcg gaagcgtctt atcatgccta catagcactg ccacgtatgt ttacaccgca
    13141 tccggcataa aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc
    13201 ctttcactcc tcgaacaatt ttcatattgt tgtttttgcg atcattatct cgacatttaa
    13261 aagcaaataa tttcattata ttttgaaatc gaaagcaaac gacaagatat gaaaatgtac
    13321 gtggtatcga caaagcagat gctgaacaac gcacagcgcg gcggttatgc ggttccggca
    13381 ttcaatattc acaatctcga aacgatgcaa gtggtggtgg aaaccgctgc caacctgcat
    13441 gcgccggtca tcatcgccgg aacgcctggc acatttactc atgctggtac agaaaatctg
    13501 ttggcgctgg tcagcgcgat ggcgaagcaa tatcaccatc cactggcaat tcatctcgac
    13561 catcacacga aatttgacga tatcgctcag aaggttcgtt ctggcgtgcg ctcagtcatg
    13621 attgacgcct cgcatttgcc ttttgcgcaa aatatttcac gggtcaaaga ggtggtggat
    13681 ttttgccatc gctttgatgt cagcgtcgag gcggagctgg ggcaacttgg cggccaggaa
    13741 gatgatgtgc aagtcaatga agtcgatgcg ttgtacacca accccgcaca ggcgcgtgaa
    13801 tttgccgagg caaccgggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg
    13861 tatgccagcg caccggtgct tgatttttct cgactggaga acattcgcca gtgggtgaac
    13921 ttaccgctgg tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc
    13981 aaactgggga tatgcaaaat caacgttgca acggagctga aaaatgcctt cttgcagtcg
    14041 ttaaaaaatt acctgaccga gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg
    14101 gctaaatccg caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc
    14161 agggcataac gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca
    14221 taaagctggt gaacatatcg gcatatgttc agtctgttct gcccatccgt tggttatcga
    14281 agcggcgctg gcatttgatc gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa
    14341 ccaggtcaat caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt
    14401 ttttacgatt gccgataaag ttgggtttgc acgcgaacgc attattctcg gcggcgatca
    14461 tctgggacca aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga
    14521 gctggtaaaa gcatatgttc gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc
    14581 ctgcgcgggg gataccatac cgttagcacc agaaacggtt gcggaacgag ctgctgtgct
    14641 ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat
    14701 tggcactgaa gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac
    14761 ccatgttgaa gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg
    14821 gctgacagag gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga
    14881 tcacagcaat attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga
    14941 aaacacccga atggtttatg aagcacattc taccgattac cagacccgga cggcttattg
    15001 ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa cctttgcttt
    15061 acgcgaggcg atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg
    15121 cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa
    15181 atattatcgc acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg
    15241 tattcgttat tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa
    15301 tctgcaaggc gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga
    15361 acgcattcag tccggggaat tatcagcaat gccgcatcag cttattatga ataaaattta
    15421 tgatgttttg cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct
    15481 gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa
    15541 tgagatgctc gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga
    15601 agcagaattc cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga
    15661 ggcgattcat gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt
    15721 tcagcaagcg gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga
    15781 aaatccgcag cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga
    15841 tatcgtcgag acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta
    15901 tgttttagat tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg
    15961 caagattatt gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat
    16021 taaagagttg tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga
    16081 aatagaaacc tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag
    16141 ttttggcgat attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc
    16201 attacaaaat aaaattctga ctatcttaca gggatgacct atgttttcag aagtcatgcg
    16261 ttatattctc gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa
    16321 aatattaggc atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt
    16381 tgttggcatt ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc
    16441 gatggcggaa aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc
    16501 accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa
    16561 cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg
    16621 gcatatgacc ttcaccggcg cgttgctgca cctggcaacc ggttcatgga tgatagggat
    16681 ggcgggtgtg gtaattcacg cggcgtttgt ttataagctc ggcgactggt ttgcccgcga
    16741 tacccgaaat ttctttgagc tggaaggtat tgctattccg cacggtacgt cggcgtatat
    16801 ggggccgatt gcggtgctgg tcgatgctat catcgagaaa atcccaggcg ttaaccgaat
    16861 taaatttagc gccgacgata ttcagcgcaa atttggccct tttggcgaac ctgtcaccgt
    16921 gggttttgtg atggggctga ttatcggcat cctcgcgggt tacgatgtca aaggtgtatt
    16981 gcagctggcg gtaaaaacgg cggcagttat gctgctgatg ccacggatga ttaaacccat
    17041 catggatggt ttaacgccca tcgctaagca ggcccgtagt cgtttacagg cgaagttcgg
    17101 cggtcaggag ttcctgattg gcctggatcc cgcattactg ctggggcata cggcggtggt
    17161 atcggcaagc ctgattttta ttccgctcac cattttaatt gctgtttgtg ttccgggtaa
    17221 tcaggtactg ccgtttggcg atcttgccac tatcggcttc ttcgtggcga tggcggtcgc
    17281 ggtgcatcgt ggaaatctgt tccgcacctt aatctcgggt gtcatcatta tgagcatcac
    17341 cctgt
//
LOCUS       DAJTYL010000086        17003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYL010000086 DAJTYL010000000
VERSION     DAJTYL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.086"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..480)
                     /gene="chbR"
                     /locus_tag="OO572_002782"
     CDS             complement(<1..480)
                     /gene="chbR"
                     /locus_tag="OO572_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6661722.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     "
     gene            complement(488..838)
                     /gene="chbA"
                     /locus_tag="OO572_002783"
     CDS             complement(488..838)
                     /gene="chbA"
                     /locus_tag="OO572_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6661723.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(897..2245)
                     /gene="chbC"
                     /locus_tag="OO572_002784"
                     /pseudo
     CDS             complement(897..2245)
                     /gene="chbC"
                     /locus_tag="OO572_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2330..2650)
                     /gene="chbB"
                     /locus_tag="OO572_002785"
     CDS             complement(2330..2650)
                     /gene="chbB"
                     /locus_tag="OO572_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6661724.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2949..3287)
                     /gene="osmE"
                     /locus_tag="OO572_002786"
     CDS             complement(2949..3287)
                     /gene="osmE"
                     /locus_tag="OO572_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6661725.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3489..4316
                     /gene="nadE"
                     /locus_tag="OO572_002787"
     CDS             3489..4316
                     /gene="nadE"
                     /locus_tag="OO572_002787"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6661726.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4546..5433
                     /gene="cho"
                     /locus_tag="OO572_002788"
     CDS             4546..5433
                     /gene="cho"
                     /locus_tag="OO572_002788"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6661727.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5393..5968)
                     /gene="ves"
                     /locus_tag="OO572_002789"
     CDS             complement(5393..5968)
                     /gene="ves"
                     /locus_tag="OO572_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6661728.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6171..6656)
                     /gene="spy"
                     /locus_tag="OO572_002790"
     CDS             complement(6171..6656)
                     /gene="spy"
                     /locus_tag="OO572_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6661729.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6986..7954)
                     /gene="astE"
                     /locus_tag="OO572_002791"
     CDS             complement(6986..7954)
                     /gene="astE"
                     /locus_tag="OO572_002791"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6661730.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7947..9280)
                     /gene="astB"
                     /locus_tag="OO572_002792"
                     /pseudo
     CDS             complement(7947..9280)
                     /gene="astB"
                     /locus_tag="OO572_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9277..10755)
                     /gene="astD"
                     /locus_tag="OO572_002793"
     CDS             complement(9277..10755)
                     /gene="astD"
                     /locus_tag="OO572_002793"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6661731.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10752..11786)
                     /gene="astA"
                     /locus_tag="OO572_002794"
     CDS             complement(10752..11786)
                     /gene="astA"
                     /locus_tag="OO572_002794"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6661732.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11783..13003)
                     /gene="astC"
                     /locus_tag="OO572_002795"
                     /pseudo
     CDS             complement(11783..13003)
                     /gene="astC"
                     /locus_tag="OO572_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13449..14255
                     /gene="xthA"
                     /locus_tag="OO572_002796"
     CDS             13449..14255
                     /gene="xthA"
                     /locus_tag="OO572_002796"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6661733.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14422..15132
                     /locus_tag="OO572_002797"
     CDS             14422..15132
                     /locus_tag="OO572_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6661734.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLHFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15137..15814
                     /gene="ydjY"
                     /locus_tag="OO572_002798"
     CDS             15137..15814
                     /gene="ydjY"
                     /locus_tag="OO572_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6661735.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15829..16536
                     /locus_tag="OO572_002799"
     CDS             15829..16536
                     /locus_tag="OO572_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6661736.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16536..>17003
                     /locus_tag="OO572_002800"
     CDS             16536..>17003
                     /locus_tag="OO572_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6661737.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGL"
BASE COUNT         4213 a         4529 c         4148 g         4113 t
ORIGIN      
        1 atgacgtaaa cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt
       61 ttcgcggaaa tttaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt
      121 ccggtatacc tgcgaagcaa caaagcaata aggcaacaac ggcaggtaat gctgctcaaa
      181 aaagcgttta ctgatcccaa cgttcaatat gcgcgtggca ccaaactcat aaaaactttg
      241 atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc
      301 gttaatctcc tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg
      361 atgcagtccg ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt
      421 aaacaactgc tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat
      481 cgactcctta tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg
      541 caagcatgga cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct
      601 taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg
      661 ccatccgtga ctgatccatc atggctttcg ctgcggcaaa atcgccctgc ttcgcctgtt
      721 tcaatgcggc atacgccagg ctacgcgctt gtccagagtt gatgataagc cccatcacca
      781 cttcttccag ctcttcagct tctgtttgcg tatcaggaat attatcgaga tccatcatac
      841 atcgttcctc tctcttctta ccggcaagat tacccgtacc ggcatcgatt aaaatttcag
      901 gggttagcga tatcttcttc gctctcttct ttatcaatcg cattttgtgc tttgttagcc
      961 accacaacaa agggcagata aattaacgtt gcgatgccaa ggttgaagag tgcgaccagc
     1021 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
     1081 ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc
     1141 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
     1201 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
     1261 acctgacgat aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc
     1321 gcaccactgc ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
     1381 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
     1441 tccagcgccc acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
     1501 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc
     1561 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
     1621 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat
     1681 gccgagaaag aacgcgatac tgaagcaggt acgctgtcgg gcagtttaat gacccaattg
     1741 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
     1801 atattcgccc cacctaacca gtttgcgcca accgcatagg cctcaccgac actatatggg
     1861 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
     1921 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat tcctaatgtt
     1981 ccgttatata cgttgccgcc aatacctttc agaccattaa gtgtttcaat ggttgaggcg
     2041 tcgaggcgaa tacctaagga ataaaaaacg acccctcccc aaagcttaga aaaacgttgt
     2101 taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata aagccatttt
     2161 tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt
     2221 caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt
     2281 acggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat taatttgctg
     2341 cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg actttgccat
     2401 aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg atttcgggca
     2461 acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct ttttcaccag
     2521 ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct gcctgtgcgc
     2581 gcatttttga taccagtaaa gagatagaca tgcccgcaga acaaaacaga taaatgtgtt
     2641 tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact ccgcaagcct
     2701 tgacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa
     2761 aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa ttgtttctcc
     2821 ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa gataattaag
     2881 aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agctttcaga
     2941 cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg
     3001 gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca
     3061 cgttgaccca ggatgtaggt ctggcaagta ccgcgagcat ggatcatgct cacttcagac
     3121 gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc
     3181 accacaggct gtacaaactg gtctttggta cgatcataag ctgtacaacc cgccagcatg
     3241 gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctctt
     3301 gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag cccgtgaacg
     3361 aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt
     3421 cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt tctgtctgga
     3481 ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta
     3541 atgctgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg caaacttatc
     3601 cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggaa
     3661 agctgtgcca gatggcgatt aatgagctgc gccaggaaac tcgcaacgaa tcactgcaat
     3721 ttattgccgt acgcctgccc tatggtgttc aggctgacga acaagattgc caggatgcca
     3781 ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcta
     3841 gcgagcaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt ggcaatgaaa
     3901 aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg
     3961 tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg
     4021 gtacggatat taatccgctg tatcgtctca acaaacgcca gggtaaacag ttactgacgg
     4081 cattaggttg cccggaacac ctttataaga aagccccaac tgccgatctt gaagatgatc
     4141 gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc
     4201 tggaagggaa aaacctacct gaacaggtcg ccagaacaat agagaactgg tatctgaaaa
     4261 ccgaacataa acgccgtccg ccaattaacg ttttcgatga tttctggaaa aagtaataat
     4321 ttgcacctat tggattgtgc gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg
     4381 ccttattcta cctacaaaac tatgcaaatt caatacattg caggagtcga ataggcctga
     4441 caggcgtagc acgtcagacg gtgtaacctt tgtcatcaac ccgcctcttt tttaatcgcc
     4501 tcccgcctgt tacactggat agataaccag cattcggagt caacagtggt acggcgttta
     4561 acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacat
     4621 tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac
     4681 accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta
     4741 cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg gatctgcacc
     4801 gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg
     4861 tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc
     4921 gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct gtttggcctg
     4981 tttgccaata ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga acaaaaactt
     5041 tgttatggcc tgttgggact ggaaccgtta agtcgcggtc gtgcatgttt tcgttcagcg
     5101 ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc
     5161 ttgcgccagt ctctggagcg tttgcgagtg gtgtgttggc cttggcaagg tgcggtggca
     5221 ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg
     5281 ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc cgggtttgat
     5341 cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa
     5401 cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg
     5461 gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cacgccctgg tcggtagtga
     5521 gcagtttgtc gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc
     5581 caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgcg
     5641 tcataatatt gaaatccatc gacatctgcc ctgccgtcag tttcgctttt accacctggt
     5701 ccgccgtaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagcgg tctgcgcttt
     5761 caaggagcat ctcgccgcct tccagcaacg tcactatcct ttccatgccg ggaaacaaag
     5821 aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg
     5881 ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac agattcaccg
     5941 acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat
     6001 cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag
     6061 gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta
     6121 ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca
     6181 gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc aaaattagca
     6241 ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg
     6301 tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc ttcagctttt
     6361 gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc
     6421 ggacgtttca tctggtcacg ctggcctttc atgatttcgt ggatctgctg tttctgcgcg
     6481 tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg
     6541 tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg
     6601 ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc
     6661 tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag
     6721 attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta
     6781 aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagag
     6841 aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
     6901 aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa cttaataccc
     6961 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
     7021 atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa
     7081 aacgttcctc tccgtcctgc gccagcaatg ttcctttctc aaacggcata aaattcagcg
     7141 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
     7201 gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
     7261 ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc
     7321 caagttccaa cgtacaggcc agcgcgccaa aatgtctgac gctgaaatgg gtaaacgtac
     7381 caccaggctc ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagaa
     7441 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc
     7501 cacgaattgc ggtatgtaga tcaaggtgcc aacgcacaga ttctttgccc tggtcataaa
     7561 aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt tcagcaaata
     7621 gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct
     7681 tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg atctcgccgt
     7741 gagatatcgc gccaagcaac gcgtccagca tttccacagg tgccgtctca ttaccgtgga
     7801 tccccgcaga aatcaccagc gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
     7861 catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata accggttttt
     7921 tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
     7981 taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc
     8041 agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc
     8101 cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg
     8161 cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc gccattcgcc
     8221 atgctttcac gtaagtcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt
     8281 tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc
     8341 atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga caccgcatcc
     8401 gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgtgcca
     8461 gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg cggttactca
     8521 cggcaatcac gtcattatga aaaacgctct ggtcgataac gtccgggttt tgctgggcga
     8581 aaatcacctg ttggggattc acctgattaa gccttgccac cgcctcgctg gcttcgcgag
     8641 tctgtcgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc ccgtagacaa
     8701 aaagtagcat acccggttca ccgtaatgac cgccgagacg attgtggttt gccgccccct
     8761 catcaccgag caacgctacc tgcggcaacg ccgaatggac gctaaatttc tcttcgtcct
     8821 taaaaatcgc ttttaacagc gattcagtga cgggcgcttc cagcgaacgg tgaaatttat
     8881 tgttcaggtt ggcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga
     8941 tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa agccagtgcg
     9001 gtgcctggcg tgcaactttt tccagtacct gctcatcgct accactgaat cccaactgac
     9061 gcagcaccgg aataaacgga cgctcgtgcg gcggaatcac ggcctggggg aatcccgcat
     9121 cggcaagggc tttcattttc agtaagccct gttttgccgc cagtcgcggg ttagacacct
     9181 gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa tgatgcgtca
     9241 gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct catcggaaaa
     9301 atccagcccg gggttaagcg tggcgggcaa tgttaacgag tccgactcca ggctcgccat
     9361 aggccatgcg cagtaatctg cggcatacca ggcgctgggg cgatggttac cggaagcacc
     9421 aatgccgccg aatggggcgg tactggcagc accagtaagc ggtttgttcc agttaacaat
     9481 ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc
     9541 gcaaaagagt ccgaagcgag tgttgttcgc catccgaatc gcctcatcaa aagtatcata
     9601 acgccagacg cgcaaaaacg gcccgaacac ctcttcatcc ggcacaccgc caacgcccgt
     9661 catttcaatg atccctggtg tcagcaacga tgtccctgct tgtaataagc gcggcgcaag
     9721 cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct gctgtgcggc
     9781 ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt tgcccggcgt
     9841 taatcgctgg ctgacggcaa ccagactagc aagaaacgca tcgccctgcg ctccgctttt
     9901 gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg ccgactgaat
     9961 ggtcagatgg acagccgcgt cgatatccgc cacctcatcg ataattagcg gattattacc
    10021 gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat gcagctggta
    10081 gccggtattg gcgctaccgg taaacagcaa accgtcgaga tcctccagcg cactcagcgc
    10141 ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca agccagcctg
    10201 ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg gtttaaagat
    10261 aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat gaccagggaa
    10321 attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcacc
    10381 gtccggcatt tcactacgct gctcgccggt acgaacgtga tacgccttaa ttgatatcgc
    10441 gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct tacccgtttc
    10501 tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg caaagcgttc
    10561 gacaacgacc tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg ccgcacgggc
    10621 tgcccgacaa gcctgttcga cctgagcggc atcggcatca ttgccttgcc ataacacctc
    10681 gcccgatacc ggattacgct tcacacgcga tgcgccctgg cccgttatcc agtcaccgtt
    10741 aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc
    10801 cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggttgcc
    10861 ggatcggtac gcgccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag
    10921 gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg acttttacgg
    10981 atggcgcgca cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa gatgtcgata
    11041 tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga
    11101 tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgggtata gatcggatgt
    11161 ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcggcgcgg
    11221 ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt
    11281 tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc
    11341 gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccagtcc
    11401 gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact aagaaacagc
    11461 gtcggcaatg cgttatagac attcagctct tttgaggcgt gaaccaacgt gccgacgcga
    11521 tagttgtacc agggatcgtt cagcccaacc gccacctcaa tagcgcaaat ccccgccacg
    11581 gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcgctttt gggcagttca
    11641 ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc attggcggga
    11701 agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga gacatctgag
    11761 cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcag
    11821 ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa
    11881 aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc gcttccagag
    11941 agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc aacaaaccta
    12001 agccgcgaac ttcactgaac aaaccatagc ggtgattaat agtattaaga cgctcaacaa
    12061 accagtcgtg acgctgttta acgccattaa gcatctctgg tgtgttgatg agctccagca
    12121 ctttgcctgc taccgccgag gccagcggat taccgccata ggtggtgcca tgagtgccaa
    12181 cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc accgaccggg aaaccgccgc
    12241 ccagcgcttt ggcggtggtt aacagatcgg gcgtcacgcc gtagtgcata taggcataca
    12301 gctcaccagt gcgcccgacg ccggtttgta cctcatcaaa aatcagcaag gcattgtggc
    12361 ggtcacacaa ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc acaccgcctt
    12421 cgccctagat gggctcgaca atcaccgcac aggtggcgtc gtcaatcagc gcgctggcag
    12481 agttaatatc gttatatgca gcatgacgaa tatccgccgg cagtggcgca aaatcctgtg
    12541 aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga aacgcatttt
    12601 tgaacgccac gatgccactc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca
    12661 gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg cgatcggcaa
    12721 acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc
    12781 cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc ggatgcgcat
    12841 ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat
    12901 cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg taaacaggta
    12961 tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa
    13021 ataattcgtt atttgtatgt taacaataag taatgtttgc gctgtaaatg tagattgcag
    13081 ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggctaactgg aaatcaagga
    13141 gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt gcgatgtggg
    13201 tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt ctagaattgt
    13261 gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc gcaccaaaag
    13321 cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat cacttaacaa
    13381 caggcggtaa gcaacgcgaa attctgctac catccacgca ctctttatct gaataaatgg
    13441 cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga cctcaccagc
    13501 ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag acaaaagttc
    13561 atgacgatat gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg
    13621 ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc
    13681 gcggctttcc cggtgacgac gaagaggcgc agcggcggat tattatggcg gaaatcccct
    13741 cactgctggg taatgtcacc gtgatcaacg gttacttccc gcagggtgaa agccgcgacc
    13801 atccgataaa attcccggca aaagcgcagt tttatcagaa tctgcaaaac tacctggaaa
    13861 ccgaactcaa acgtgataat ccggtactga ttatgggcga tatgaatatc agccctacag
    13921 atctggatat cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc ggtaaatgct
    13981 ctttcctgcc ggaagagcgc gaatggatgg acaggctgat gagctggggg gtggtcgata
    14041 ccttccgcca tgctaatccg caaacagcag atcgtttctc atggtttgat taccgctcaa
    14101 aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc caaccgctgg
    14161 cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa aaaccgtccg
    14221 atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct ggctcaaact
    14281 gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg
    14341 ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc tattatcaag
    14401 tttgatatag gaaactccac gatgaacgct gagcgtaact ttctgtttgc ctgtcttatt
    14461 tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct caccgattta
    14521 ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct ctatattctg
    14581 ttattcatca atgcccccct ctttctgtta ccaggaagca tactggtgat cgctggagga
    14641 atagtttttg gcccgctctt agggacacaa ctctcattaa ttgccgcaac gctggcctcc
    14701 tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa atacgtgggc
    14761 catagccata ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat tgattttctt
    14821 attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc ttacggattc
    14881 accaccatcg ccttctggcc ttataccctt atttcggcac tcacgaccct gcccggtatt
    14941 gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct gcactttatt
    15001 ttacaactct gtctggcggg cctggcgctg tttattctca tccagctcgc aaaactctac
    15061 gcccgacaca aacatgtgga tctgtctgct tcgcgccgca gcccacttac tcacccaaaa
    15121 aatgaaggat agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag gactggctgc
    15181 actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga atgcggcggc
    15241 aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg ccaataacca
    15301 tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca ctcgtcacgg
    15361 tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatggctt atgcgccccc
    15421 gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa acatgacgat
    15481 ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg tcaactggca
    15541 aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg gcaagaaact
    15601 ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg gttgcctggt
    15661 gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt atggtgcggt
    15721 ggaaaaacac ggcgaagtga aattcaaagg caatgcctca gttctcccgg cggataacac
    15781 gctggcaacg gttaccttta aaatcaccga ataaagccaa gataaaggat gatgaagata
    15841 caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt cgctatgctg
    15901 ctcgcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt tgcggcgttt
    15961 gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc actggcggca
    16021 gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc tgcgtttttg
    16081 atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct gtcgtggacc
    16141 agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg ccgcgaagtg
    16201 gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg acggcttttt cactcgctac
    16261 ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga tccaatcagc
    16321 tatgctgccg gtctgacttc aatacgtttt cgctcgtttt ttatcgccac cgggcttggt
    16381 caattaccgg cgactattgt ttattcctgg gcaggcagca tgttaacagg cggtactttc
    16441 tggtttgtca ccgaactgtt tattctgttt gccctgaccg tggtgatttt tatggcgaag
    16501 aaaatatggc ttgagcgcca gaagaggaat gactgatggg tttaccgccg cttagcaaaa
    16561 ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc gaggtgctaa
    16621 gcccgattcg ctggtggggg cggatcccgt ttatctttta tttggtgtca atgtttgttg
    16681 ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt gtcagcgcgc
    16741 gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat caccagtatg aaagtcgccg
    16801 agcgtaccgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa agcccgctct
    16861 ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc acaccgccaa
    16921 cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag gcgctcaccg
    16981 aactgacggc attgatcggc ctg
//
LOCUS       DAJTYL010000087        16759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYL010000087 DAJTYL010000000
VERSION     DAJTYL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.087"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            129..1262
                     /gene="fepE"
                     /locus_tag="OO572_002801"
                     /pseudo
     CDS             129..1262
                     /gene="fepE"
                     /locus_tag="OO572_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1259..2074)
                     /gene="fepC"
                     /locus_tag="OO572_002802"
     CDS             complement(1259..2074)
                     /gene="fepC"
                     /locus_tag="OO572_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6661738.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(2071..3063)
                     /gene="fepG"
                     /locus_tag="OO572_002803"
     CDS             complement(2071..3063)
                     /gene="fepG"
                     /locus_tag="OO572_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6661739.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3060..4064)
                     /gene="fepD"
                     /locus_tag="OO572_002804"
     CDS             complement(3060..4064)
                     /gene="fepD"
                     /locus_tag="OO572_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6661740.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4175..5407
                     /gene="entS"
                     /locus_tag="OO572_002805"
     CDS             4175..5407
                     /gene="entS"
                     /locus_tag="OO572_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6661741.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5411..6367)
                     /gene="fepB"
                     /locus_tag="OO572_002806"
     CDS             complement(5411..6367)
                     /gene="fepB"
                     /locus_tag="OO572_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6661742.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6659..7834
                     /gene="entC"
                     /locus_tag="OO572_002807"
                     /pseudo
     CDS             6659..7834
                     /gene="entC"
                     /locus_tag="OO572_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7844..9454
                     /gene="entE"
                     /locus_tag="OO572_002808"
     CDS             7844..9454
                     /gene="entE"
                     /locus_tag="OO572_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6661743.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9468..10325
                     /gene="entB"
                     /locus_tag="OO572_002809"
     CDS             9468..10325
                     /gene="entB"
                     /locus_tag="OO572_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6661744.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10325..11071
                     /gene="entA"
                     /locus_tag="OO572_002810"
     CDS             10325..11071
                     /gene="entA"
                     /locus_tag="OO572_002810"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6661745.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11074..11487
                     /gene="entH"
                     /locus_tag="OO572_002811"
     CDS             11074..11487
                     /gene="entH"
                     /locus_tag="OO572_002811"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6661746.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11668..13773
                     /gene="cstA"
                     /locus_tag="OO572_002812"
     CDS             11668..13773
                     /gene="cstA"
                     /locus_tag="OO572_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6661747.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13955..14152
                     /locus_tag="OO572_002813"
     CDS             13955..14152
                     /locus_tag="OO572_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6661748.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14162..15250)
                     /gene="hcxA"
                     /locus_tag="OO572_002814"
     CDS             complement(14162..15250)
                     /gene="hcxA"
                     /locus_tag="OO572_002814"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6661749.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15359..16519
                     /gene="ybdL"
                     /locus_tag="OO572_002815"
     CDS             15359..16519
                     /gene="ybdL"
                     /locus_tag="OO572_002815"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6661750.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16520..>16759)
                     /locus_tag="OO572_002816"
     CDS             complement(16520..>16759)
                     /locus_tag="OO572_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6661751.1"
                     /translation="EGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIG
                     KELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
BASE COUNT         3704 a         4386 c         4795 g         3874 t
ORIGIN      
        1 taaatagagt gaatgttgca ttcagtggaa ggaatttaca aacttcagct tcggataatt
       61 ctcaccaact ggtaaagtga gcgccgtgga gattttcccc tattggactc attttcaggt
      121 tataccccat gtcatcattg aatattaaac agggaagtga cgctcatttt tctgagtatc
      181 ctctggcgtc gcccagtaat aatgaaattg atttacttaa tctaatcgaa gttttatggc
      241 gggccaaaaa aacggtcatg gcggtcgttt ttgcatttgc ctgcgcaggt ttactgatct
      301 ctttcatcct gccgcaaaaa tggaccagct cagccgttat tactcccgcc gaagccattc
      361 agtggcagga cctggagaaa acctttacca aactccgcgt tcttgatctc gatgttaata
      421 tcgaccgtgg cggtgcattt aacctgttta tcaagaagtt tcagtctgtt agcttgctgg
      481 aagagtacct gcgttcatca ccttatgtga tggactaatt aaaagaggcg aaaatcgatg
      541 aactggattt gcatcgcgct attgttgcat tgagcgaaaa aatgaaagcg gttgatgaca
      601 atgctagtaa gaaaaaagat gaaccgtcac tgtatacatc ctggacgcta agttttaccg
      661 cgccaaccag taaagaggcg cagaccgttt tgagcgggta tatcgattat atctctgcgt
      721 tggtggtgaa agagtcgata gaaaacgtcc gtaataaact ggagatcaaa acccagtttg
      781 aaaaagaaaa actggctcag gatcgcatta aaacgaaaaa tcaacttgat gcaaacattc
      841 agcgcctcaa ttattcactc gacattgcca acgcggcagg aattaaaaag cccgtataca
      901 gtaatggtca ggccgttaaa gatgaccccg atttctctat ttccctcggc gcagacggta
      961 ttgaacgcaa actggaaatt gaaaaagcgg ttactgacgt tgcggaactg aacggtgaat
     1021 tacgtaatcg gcagtatctt gtcgagcaat taacaaaagc aaatgtcaac gatgtgaatt
     1081 ttacgccgtt taaatatcag ttaagcccgt cattgccagt gaaaaaagac ggtccgggta
     1141 aggcgattat tgtgatcctt tccgcgctga tcggcgggat ggtggcttgt ggtagcgtgt
     1201 tattgcgcta tgcgatggca tccagaaaac aggatgccat gatggcagac cacttagttt
     1261 aactatttgc ggttgaaggt gccgttcgtc cgagcggcac cacaagcggc attccggcta
     1321 ccggatcgtc aatgatcatg catcgcagac cataaatgcg ctcaatcagt tcagcagtga
     1381 caatctcctt cggcgctccc tgagcaacaa ttttcccttc ccgcaatgca atcaaatggc
     1441 tggcgtaacg acaggcctga ttaagatcgt gcagcaccgc cgccagggta tagcctttct
     1501 cgcggttcag ttcgcttaac agttccagta gatcaatctg atgactgata tccagccagg
     1561 tcgtgggttc gtcgagcagc ataattgccg tttcctgggc cagcaccatc gcgatccacg
     1621 ctcgctggcg ttgtccgcca gaaagggtat ccacgctttg atctgccaga tgagttattc
     1681 ccgtggcctg catcgctttc gttaccgctt cttcatcctc tttgcgccag cgggtaaaca
     1741 gcggttgatg cggataacgt ccacgcgcca ccagctcctg cacggtgata tcgcccggcg
     1801 tggtagcatt ttgcgccaac agaccaatcc ggcgtgcaac ttctttactg gcgtaatgtt
     1861 gaatgtgctc gccatccagc cagacatgcc cgtgagcagg cgtcatcagg cggcttaagg
     1921 tacgcagtaa cgtggattta ccacagccat ttggtccgat aattgccgtg aagtgaccat
     1981 caggaatttc tacagtcaga ttttccgcaa cggtatattt gccatatccc agggttaact
     2041 gttcgccgcg caaacgggct actgattcgg tcattttttg cgagactcct gaattaacaa
     2101 gacgataagg taaataccgc cgaggctgac ggtaacgacg ccaaccggaa gttgatacgg
     2161 cataaacagt tgttgggcgc agagaacggc cgccagcagt aacagcgctc cgcacagcgc
     2221 cgcctgggtc agcccccagc gagcggtgcc gctaatgcgt cgggcaatgt gcggcgcgac
     2281 taaagcaata aaggaaatcg gcccggcaag cgccgttgcc gcagcggtaa gcaccactgc
     2341 aaccagcatc attaacagac gcgaacgctc gacgctgacg cccagcgcgc aggcggtatc
     2401 atcgcccatc tccagcaagc gcattcgccg caccagcaag gtggcggcaa tgagcatcaa
     2461 tataatgatg ggtgcggaag gcgaggtttt tgcccacgtc aggccgttga gcgatccggc
     2521 attccacaaa cctgctgtta gcgccgtttc taaagacgct ttcagcaaca gccaggtatt
     2581 aaaggccacc agcatggcgc gaacgccgat accgataata atcaaccgaa aggtgtcgat
     2641 accgttacgc caggcgagca gccagaccaa cagcgaagtg ataatgccgc ccaccattgc
     2701 tgccagcgcg atagccgtca gatcctgacc aaacagcacc atcgccacca gcacgccgct
     2761 ccacgccccg gtgttaaagc ccattacgtc agggctgccg agcgggttac gcatcagcga
     2821 ctgaaaaatc gcgccgctga cgcccagcgc cgcgccaatc aacagcgcca tcagcacgcg
     2881 tggtaaacgc cattcggtga ccaccatcgt catactgcgc ggcgcatcgc ccatcaacgc
     2941 ggcgaatacc tgcgagattt ccagcgtgac ggcaccgctg cgtaatcccc agatacctgc
     3001 aaccacacag gcggaaatca gcagcaaaca ggtgatgagt aatcggcgag agacgtaaat
     3061 catgcaccac ctcgcgtttt acgtcgcacg aggaagatca gcaccggtgc accaataaac
     3121 gcactgacca cagaaacgcg cagttcgccg ggcacaatca cccgcccgat gatatcggca
     3181 aacagcagca gggcaggggt agcaagtagc gtgacgggca gcgaccagcg atgatcggca
     3241 cctaccagcc agcgcgccat gtgcggcatc atcaggccga taaaggcaat cgggccaact
     3301 accgccgtcg cactaccaca aagcacggta atcgccagca gaccaatcaa ctgtgtgcgc
     3361 gccacgcgac tgcccagcgc cgtcgcggtg tcactgccga ggctcaaact gttcagcgcg
     3421 cgactcagta atagcgcagt tgctccggcg atcagaaccg ggatcagcac cacttttaag
     3481 gtatgtagat tgcgaatatc cagcgaaccg gcttgccaga aacgcagttg atcgtagacg
     3541 tcaggattaa gcagggcgat gccgctggtc agtccttcca gcaccgccgc cagcgccacg
     3601 cccgccaggg ttaaacgcac cggacttaac tgccctccac cctgactgcc ggtaaaggca
     3661 acaatcaatg aggccaccag cgccccggcg aaggccatcg ccagttgttc ctgtgcggat
     3721 gagtaaccaa acagcgccgc acccagcaca atggcaaagc tggctccggc gttcacgcca
     3781 agcaagccgg ggtcggcaag tgggtttcgg gtgagggttt gcattaacgc cccggcaagg
     3841 ccaagcgcgc cgcctgccag taaaccggca agggtacgcg gcagccgcgc gtcgagcacg
     3901 atggtgcagt cggcgctctg gcaggtgccg gagaaggcct ccagcactac ggaagcgggg
     3961 agtgattttg ccccaatgag caggcttaat gccgtcgcga taatcagtaa taacagcaat
     4021 ccgggcacgg caatggcgcg tgtcacggca acagaaccag atatacgaac ttccatgata
     4081 atgaaattaa ttatcgttat cgatcttatt tggatatgtt aacatgtgca gcctaagaat
     4141 aggtatttaa aatatttgat ggcaaggcat tgtaatgaat aaacaatcct ggctgcttaa
     4201 cctcagcctg ttgaaaacgc acccggcgtt tcgcgcagta ttcctcgctc gttttatctc
     4261 aattgtgtct ctgggtttgc tcggcgtcgc ggtgccggtg cagatccaga tgatgaccca
     4321 ttctacctgg caggtggggc tttcggtgac gctgaccggc ggcgcgatgt ttgttggcct
     4381 gatggttggc ggtgtgctgg cggatcgcta tgaacgcaaa aaagtgattt tgctggcgcg
     4441 cggcacctgt ggcattggct tcattggact gtgccttaat gcactgctgc cggagccgtc
     4501 attgctggca atctatttac ttggtttatg ggatggtttt ttcgcatcac ttggtgttac
     4561 ggcgctactg gcggcaacat ctgcactggt agggcgtgaa aacttaatgc aggccggggc
     4621 gatcaccatg ttgaccgtgc gcctggggtc ggtgatttcg cccatgattg gcggtttatt
     4681 gctggcgacc ggcggcgtag cctggaacta cgggctggcg gcggcgggca cgtttattac
     4741 cttgttaccg ttattaagcc ttccggagtt gccaccgcca ccgcagccgc ttgagcatcc
     4801 gttgaaatca ttactggcag gatttcgttt tctgcttgcc agcccgctgc tgggtggttt
     4861 attgacgatg gcgagcgcgg tgctggtact gtatccggcg ctggctgaca actggcagat
     4921 gtcagcggca cagattggtt ttctctacgc ggcgatcccg ctcggcgcgg ctattggtgc
     4981 gttaaccagc gggaagctgg cacatagtgc gcgaccaggg ttattgatgc tgctctccac
     5041 gctgggatcg ttcctcgcca ttggtctgtt tggcctgatg ccgatgtgga ttttaggcgt
     5101 ggtttgtctg gcgctgttcg gctggctgag cgcggttagt tcgttgctgc aatatacaat
     5161 gctgcaaacg caaaccccgg aagcgatgtt agggcggatt aacggtttgt ggacggcgca
     5221 gaacgtgacg ggcgatgcca taggcgcggc gctgctgggt ggtttgggcg cgatgatgac
     5281 accggttgct tccgcaagcg cgagcggttt tggtttgttg attatcggcg tgttgttatt
     5341 gctggtgctg gtggagttgc gacgttttcg ccagacgccg ccgcaggtga cagcgtccga
     5401 cagttaatgc ttaaaacagc gccttaagcc gttccagcac ttgcatggcg ctgtagtaat
     5461 ccaggcggaa cgtctcggtt cccagcgcat aaacctgctt gttttgtacc gcaggcaggt
     5521 gcgcgagcag tggattggca taaatcgcat cggcatcttt ctgatcacca gcgaacagga
     5581 atagcgattc accgtttaac cccgcagcca gattttcccc accaagctga atgatgtcat
     5641 ggcgtttgcc ctggctttgg ctggcgttta accctgcggg caatttcgcc agcgtaaagc
     5701 cgagttgttc cagcatctgc ccttgtgctg attccggcgt ccagagattg gcactgtgtg
     5761 cagcggcggt atagacaatg gcagtgaccg gctgcggcgg taatttgatt tgctctttcg
     5821 ccgctgccag ttgcttatca aactgtgcaa tccgctctgc cgcttgtttc tcatgcccgg
     5881 taatttcgcc aagctgcgtt aacagcgact gccagctttt gtcgtcgtaa ttgatgatta
     5941 atgtcggggc gatggtggaa agctgatcat acagcgccag cgccgaatcc ccgccggttg
     6001 cgctaattaa aatcagatcc ggcatttgcg cggcaacggc ttcggcgctc ggttcgccga
     6061 tatagagccg ttgcagtttg cgttctttcg ccaccttgct ccactggcgt aaaaagccct
     6121 ggtcatctgc gacgcggtta ttcggcgtgg tcgcgccgct ggcgatcacc ggagcatcaa
     6181 tcgccagcag tgatccggtc agggtgacgc tggtggaaac aatacgctgc ggctggcttt
     6241 ccagggtatg agtgccccgg ctgtcagtaa tctgacgcgg ccagtcggcg gcctgaacta
     6301 cggctattcc tgaaagcaaa agtcctgtta atagaagggc gttgcggtag agcggggcga
     6361 gtctcacaag tcagcttcct gttattaata aggttaaggg cgtaatgaca aattcgacaa
     6421 agcgcacaat ccgtcccttt ggggagaggg ggcaaaacgg cgcaggacat cacattgcgc
     6481 ttatgcgaat tcatcaataa tgcttctcat tttcattgta accacaacta gatgcaaccc
     6541 cgagttgcag attgcgttac ctcaagagtt gacatagtgc gcgtttgctt ttaggttagc
     6601 ggccgaaaat ataagtgata atcattatta aagcctttat cattttgtgg aggatgatat
     6661 ggatacgtca ctggctgagg aagtacagca gaccatggca acacttgcgc ccaatcgctt
     6721 tttctttatg tcgccgtacc gcagttttac gacgtcagga tgtttcgccc gtttcgatga
     6781 accggctgtg aacggggatt cgcccgacag tcccttccag caaaaactcg ccgcgctgtt
     6841 tgccgatgcc aaagcgcagg gcatcaaaaa tccggtgatg gtcggggcga ttcccttcga
     6901 tccacgtcag ccttcgtcgc tgtatattcc cgaatcctag cagtcgttct cccgtcagga
     6961 aaaacagacc tcagcccgct gttttacccg cagccagtcg ctgaacgtgg tggaacgcta
     7021 ggcaattcct gaacaaacca cgtttgaaca gatgggtgcc cgcgccgccg cacttaccgc
     7081 cacgccgcag gtcgacaaag tggtgttgtc acgattgatt gatatcacca ctgacgccgc
     7141 cattgatagt ggcgtattgc tggaacggtt gattgcgcaa aacccggtta gttacaactt
     7201 ccatgtcccg ctggctgatg gtggcgtcct gctgggggcc agcccggaac tgctgctacg
     7261 taaagacggc gagcgtttta gctccattcc gttagccggt tccgcgcgtc gtcagccgga
     7321 tgaagtgctc gatcgcgaag cgggtaatcg tctgctggcg tcagaaaaag atcgccatga
     7381 acatgaactg gggactcagg cgatgaaaga ggtactgcgc gaacgcagta gtgagttaca
     7441 cgttccctcc tctccacaat tgattaccac gccgacgctg tggcatctcg caactccctt
     7501 tgaaggtaaa gcgaattcgc aagaaaacgc actgactctg gcctgtctgc tgcatccgac
     7561 ccccgcgctg agcggtttcc cgcatcaggc cgcgacccag gttattgctg aactggagcc
     7621 gttcgaccgc gaactgtttg gcggcattgt gggttggtgt gacagcgaag gtaacggcga
     7681 atgggtggtg accatccgct gcgcgaagct gcgggaaaat caggtgcgtc tgtttgccgg
     7741 agcggggatt gtgcctgcgt cgtcaccgtt gggtgagtgg cgcgaaacag gcgtcaaact
     7801 ttctaccatg ttgaacgttt ttggattgca ttaaggagcg aggatgagca ttccattcac
     7861 ccgctggccg gaagagtttg cccgtcgcta tcgggaaaaa ggctactggc aggatttgcc
     7921 actgaccgac attctgactc gccacgctgc gagtgacagc atcgcggtta tcgacggcga
     7981 gcggcagttg agttatcggg agctgaatca ggcggcggat aacctcgcct gtagtttgcg
     8041 ccgtcagggc attaaacctg gtgaaaccgc gctggtacaa ctgggtaacg tcgctgaact
     8101 ttacattacc tttttcgcgc tgctgaaact gggcgtcgcg ccggtgctgg cgctgttcag
     8161 ccatcagcgt agtgaactga acgcctacgc cagccagatt gaaccggcgt tactgattgc
     8221 cgatcgccag catgcgctat ttagcgggga tgatttcctc aacacatttg ttgcagagca
     8281 ttcttccatt cgcgtggtgc aactgcacaa cgacagcggt gagcataact tgcaggatgc
     8341 gattaaccat ccggcagagg gttttactgc cacgccatca cctgctgatg aagtggccta
     8401 tttccagctt tccggcggca ccaccggcac accgaaactg atcccgcgca ctcataacga
     8461 ctattactac agcgtgcgtc gtagcgccga gatttgtcag ttcacacaac agacgcgcta
     8521 cctgtgctcg atcccggcgg ctcataacta cactatgagt tcgccaggat cgctgggcgt
     8581 ctttcttgcc ggaggaacgg ttgttctggc ggccgatccc agcgccacgc tttgcttccc
     8641 gttgattgca aaacatcagg ttaacgttac cgcgctggtg ccgccggcag tcagcctgtg
     8701 gttgcaggcg ctggctgaag gcgaaagccg ggcgcagctt gcctcgctga aactgttaca
     8761 ggtcggcggc gcacgtcttt ctgccacgct tgcagcgcgt attcccgctg agattggctg
     8821 ccagttgcag caggtgtttg gcatggcgga agggctggtg aactacaccc gtcttgatga
     8881 tagcgcggag aaaattatcc atacccaggg ttacccaatg tgtccggacg atgaagtgtg
     8941 ggttgccgat gccgaaggaa atccactgcc gcaaggggaa gttggacgcc tgatgacgcg
     9001 cgggccgtac accttccgtg gctattacaa aagtccgcag cacaatgcca gtgcctttga
     9061 tgccaacggt ttttactgtt ccggcgatct gatctctatt gatccagagg gttacatcac
     9121 cgtgcagggg cgcgagaaag atcagatcaa ccgtggcggc gagaagatcg ctgccgaaga
     9181 tatcgaaaac ctgctgctga gccatccggc agtgatctat gccgcactgg tgagcatgga
     9241 aaatgagctg atgggcgaaa aaagctgtgc ttatctggtg gtaaaagagc cgctgcgcgc
     9301 ggtgcaggtg cgtcgtttcc tgcgtgaaca gggtattgcc gaatttaaat taccggatcg
     9361 cgtggagtgt gtggattcac ttccgctgac ggcggtcggg aaagtcgata aaaaacaatt
     9421 acgtcagtgg ctggcgtcac gcgcatcagc ctgaaggaga gaacacgatg gctattccaa
     9481 aattacaggc ttacgcactg ccggagtctc acgatattcc gcagaataaa gttgactggg
     9541 cctttgaacc gcaacgtgcc gcgttgttaa tccatgatat gcaggactat tttgtcagct
     9601 tctggggcga gaactgcccg atgatggagc aggtgatcgc gaatattgct gcgctgcgcg
     9661 actactgcaa acagcacaat atcccggttt attacaccgc ccagccgaaa gagcagagcg
     9721 atgaagatcg ggcgctgttg aatgatatgt gggggccggg cctgacccgc tcgccggaac
     9781 agcaaaaggt ggtggatcgc ctgacgccag atgccgacga cacggtgctg gtgaagtggc
     9841 gctacagcgc gtttcatcgt tctccgctgg agcaaatgct caaagagagt ggacgtaacc
     9901 agctgattat tacaggagta tatgcccaca ttggctgtat gaccaccgca accgacgcat
     9961 ttatgcgcga tattaaaccg tttatggtgg cggatgcgct ggctgatttc agccgtgacg
    10021 agcatttgat gtcgctgaaa tatgtggccg gacgttctgg ccgggtggtg atgaccgaag
    10081 aattactgcc agcacctatc cccgccagca aagcggcgct gcgtgaggtg atcctgccgt
    10141 tgctggacga gtccgatgaa ccgttcgatg acgacaacct gatcgactac ggtctggatt
    10201 cggtgcgcat gatggcgctg gcggcgcgct ggcgcaaagt gcatggtgat atcgactttg
    10261 tcatgctggc gaaaaacccg accatcgacg cctggtggaa gctactctcc cgcgaggtga
    10321 aataatggat ttcagcggta aaaatgtctg ggtaaccggc gcaggtaaag gtatcggcta
    10381 cgccacggcg ctgacgtttg ttgaggcggg agcgaaagtt acaggttttg atcaagcgtt
    10441 cactcaggag caatatccct ttgcgaccga agtaatggat gttgccgacg ccgggcaggt
    10501 ggcgcaggta tgtcagcgac tgttagctga aacggagcga ctggacgtgc tgatcaatgc
    10561 ggcgggaatt ttgcgcatgg gcgcgaccga tcagctcagt aaagaggact ggcagcagac
    10621 ttttgcggtt aacgtcggcg gtgcgtttaa cctgttccag caaaccatga accagtttcg
    10681 ccgtcagcgg ggcggggcga ttgtcactgt ggcgtccgac gccgcacaca cggcgcgtat
    10741 tggtatgagt gcttatggcg catcgaaagc ggcgctgaaa agcctggcgt tgagcgtcgg
    10801 gctggaactg gcgggtagcg gcgtgcgctg taatgtggtt tcgcctggct ccaccgacac
    10861 cgatatgcaa cgcacgctgt gggtgagcga tgacgccgaa gaacagcgta ttcgcggctt
    10921 tggcgagcag tttaaactcg gcattccgct ggggaaaatc gcccgtccac aagagatcgc
    10981 caacacgatt ttgttcctcg cctctgacct cgccagccat atcaccctac aggatattgt
    11041 ggtcgatggc ggctcaacgc tgggggcata agcatgatct ggaaacgcca tttaacgctc
    11101 gacgaactga acgccaccag cgataacaca atggtggcgc atctgggaat tgtgtatacc
    11161 cttctgggag atgatgtgct ggaagccgaa atgccggttg atacccgtac tcatcagccg
    11221 tttggcctgc tgcatggcgg cgcgtcggcg gcgctggctg aaacgctggg atcgatggcc
    11281 ggatttatga tgacccgtga cgggcagtgt gtggtgggca cggaacttaa cgccacccat
    11341 catcgcccgg tgtctgaagg caaggtacgc ggcgtctgcc agccgctgca tcttgggagg
    11401 caaaatcaga gttgggaaat cgtcgttttc gatgaacagg ggcggcgttg ctgcacttgt
    11461 cggctgggta cggcagtttt gggatgagcc cgacagaatt aaatgagatt gcaggaaaac
    11521 tcggttaacg gagtgatcga gttaacattg ttaagttaaa tattggtttc aactccgatt
    11581 tacatggtta ccgtgttgtt aaatcgtaca aagatgttat agaaacaaaa tgtaacatct
    11641 ctatggacac gcacacggat aacaactatg aacaaatcag ggaaatacct cgtctggaca
    11701 gtgctctctg taatgggagc atttgctctg ggatacattg ctttaaatcg tggggaacag
    11761 atcaacgcgc tgtggattgt ggtggcgtcg gtctgtatct atctgatcgc ttaccgtttt
    11821 tatgggctgt atatcgccaa aaatgtgctg gcggttgacc cgacgcgtat gacgccagcg
    11881 gtgcgccata acgacgggct ggactatgtg ccgacggaca agaaagtgct gttcggtcac
    11941 cattttgcgg ccattgccgg agcagggccg ctggtggggc cggtgctggc agcgcaaatg
    12001 ggctacctgc cggggatgat ctggctgctc gccggggtgg ttctcgccgg tgcggtgcag
    12061 gatttcatgg tgctgtttgt ttctacgcgc cgtgacggtc gctcgctggg tgagctggtc
    12121 aaagaagaga tggggccaac tgccggggtg attgcgctgg tagcctgctt tatgatcatg
    12181 gtcattatcc ttgcagtact ggcgatgatc gtggtgaaag ccctgactca tagcccgtgg
    12241 ggaacgtata ccgttgcgtt caccattccg ctggcgattt ttatgggaat ctacctgcgc
    12301 tatctgcgtc cgggccgcat tggcgaagtg tcagtgattg gcctggtgtt cctgattttc
    12361 gccattatct ctggcggctg ggtggcagaa agtccgacct gggcaccgta ctttgacttt
    12421 accggcgtgc agctgacctg gatgctggtg ggctacggtt ttgtggcggc ggtgctgccg
    12481 gtgtggttgc tgctggcccc gcgtgactat ctctcgacct tcctgaaaat cgggactatc
    12541 gtcggtctgg cggtaggcat tttgattatg cgcccgacgc tgaccatgcc tgcgctgacc
    12601 aaatttgtcg atggcactgg cccggtatgg accggtaacc tgttcccgtt cctgtttatc
    12661 accatcgcct gtggtgcggt gtctggcttc catgcgctga tctcttccgg caccacgccg
    12721 aagatgctgg cgaacgaagg gcaggcgtgc tttatcggct acggtgggat gttgatggaa
    12781 tccttcgtgg cgattatggc gctagtttct gcctgtatca tcgatccggg cgtgtatttt
    12841 gccatgaaca gcccaatggc ggtgctggct ccggcaggga cggcggatgt agtcgcttct
    12901 gccgcgcagg tggtgagtag ctggggcttt agcattacac cagatacgct caaccagatt
    12961 gccagcgaag tgggtgaaca gtcgatcatt tcccgtgcgg gcggtgcgcc gacgttggcg
    13021 gtggggatgg cttacatcct gcacggtgcg ctgggtggta tgatggatgt ggcgttctgg
    13081 tatcacttcg ccattctgtt tgaagctctg tttattctga cggcggtaga tgcgggtacg
    13141 cgtgctgcgc gctttatgtt gcaggatctg ctgggcgtgg tgtctcctgg cctgaagcgg
    13201 accgattcac tgcctgctaa cctgctggca acagcgctgt gcgtactggc gtggggctac
    13261 ttcctccatc agagcgtggt cgatccgctg ggcggtatta acactctgtg gccgctgttt
    13321 ggtattgcca accagatgct ggcagggatg gcgctgatgc tctgtgccgt ggtgttgttc
    13381 aagatgaaac gtcaacgtta cgcctgggtg gcgctggtac caacggcctg gctgctgatt
    13441 tgtaccctga ccgctgactg gctgaaagcg tttagcccgg atgcgaaagt cggcttcctg
    13501 gtcattgcta acaagttcca ggcaatgatc gacagcggca atattccgtc gcagtatact
    13561 gagtcacagc tggcgcaact ggtgttcaac aaccgtctgg atgccgggtt aaccatcttc
    13621 tttatggtgg tcgtggtggt cctggcactg ttctcgatta agacggcact tgcggcattg
    13681 aaagatccga agccaacggc gaaagaaacg ccgtatgagc cgatgccgga aaatgtcgag
    13741 gagatcgtag cgcaggcgaa aggcgcgcac taaagtcaga gtgagggggg atgttggcga
    13801 atgttggcat agtgccgagg gttccccctc accctaaccc tctccccgat ggggcgaggg
    13861 gactgaccga gtgcgttgat agcatttgta ggccggataa agcgttcacg ccgcatccgg
    13921 cactctttca gcaacatggt tagcggaggc caagatgttt gattcactgg caaaagccgg
    13981 aaaatattta ggtcaggcgg cgaagctgat gattggtatg cctgattacg acaactatgt
    14041 cgaacatatg cgggttaatc atcccgatca aacgccgatg acctacgagg agtttttccg
    14101 tgagcggcag gacgcgcgct acggtggaaa aggcggcgcg cgctgctgct aaatttgcac
    14161 gtcaggcttt aaacgattcc actttttcga acgctgcacg caacgtatct ggtgtcagcg
    14221 tgactggcag gtaatgaatg gactccaccg ggcgcaaggt gtgggcaatc actttgtcga
    14281 tctccgcctg attattgata tccacctcca gctccgccag cgtagtcggc agatgaaaac
    14341 gctgatacgc cccggttaat tgcgccagca catcatcctg acccagcaag gcgctttgca
    14401 ccagaattcc gtaagcgact ttggtgccgt gaagaaactt ctcggtttgc ggcaggacag
    14461 tcagaccgtt atgcacggca tgagccgccg ccacacgcgt aaaacgatcg cccagaccac
    14521 cgaccatccc accaccagca ataatagcat ccaccacatc gcaaaatgat tgcgttaact
    14581 gctgattttg ctgatcggca agtgcctgtt cgctactgtt taacaagacg tcgcgaatgg
    14641 cctgcgcatt attgatcccc agccgcacgg ttagcggcaa cgtttctggt tgcggagcca
    14701 gcaccaccgc ttcataccat ttcgccagcg tgtcaccgat ccccgccagc agatattgtt
    14761 gcggtgcatt gaggataatc tccggttcca ccagcaccat aaaattggcg tcgtcgaaaa
    14821 tctcataatg cagcgcctgt ccggcatcgt tataccagac ggagagcggt gtccaggctg
    14881 cgcaggtggc ggcgatcgtc ggagcggcaa caaatggcag accgagacgg cgggcgaggg
    14941 ctttcgcggt gtcgagcagt gcgccgccgc cgacgccaat caccacgctg cgatcgtcac
    15001 cggactcagc cgccagttgc tgtacatcgc tttcgctgca atgaccacga aacaaaatat
    15061 gctttgcccc tggaagtcca aacgctggcg gaagtttggt ttgcgccgca gcaatggcgc
    15121 gtttgccgta gatccacacc gcgcgagaaa gttgttcatc agtgaaaaaa ttgtgcaggt
    15181 gattgaaact tcctggatgt gaaaagtagt tagccgggcc gacgaccacg cggataggat
    15241 tgtgaggcat gttgtgttgt ccttattgtt ttatttagac atctaaacgt cttgattgcc
    15301 aaatactggc atcgtgttat agtgccttca acacgcaact tcgtcaggta caataaaaat
    15361 gacaaataac cctctgattc cacaaagcaa acttccacaa cttggcacca ctattttcac
    15421 ccagatgagc gcgctggcgc agcaacacca ggcgattaac ctgtcgcaag gctttcctga
    15481 ttttgatggt ccgcgctatt tacaggagcg gctggcgtac cacgttgccc agggggcaaa
    15541 ccaatacgcg cccatgaccg gcgtgcaggc cttgcgcgag gcgattgctc agaaaacgga
    15601 acgtttgtat ggctatcaac cagatgccga tagcgatatc accgtaacgg caggggcgac
    15661 ggaagcgtta tacgcggcga ttaccgcact ggtgcgcaat ggcgatgaag tgatttgttt
    15721 tgatcccagc tatgacagtt acgcccccgc catcgcgctt tctgggggaa tagtgaaacg
    15781 tatggcactg caaccaccgc attttcgcgt tgactggcag gaatttgccg cattattaag
    15841 cgagcgcacc agactggtga tcctcaacac tccgcataac cccagtgcaa ctgtctggca
    15901 gcaggctgat ttcgccgctt tgtggcaggc gatcgccggg cacgagattt ttgtcattag
    15961 cgatgaagtc tacgagcaca tcaacttttc acaacagggc catgccagtg tgctggcgca
    16021 tccgcagctg cgtgagcggg cagtggcggt ttcttcattt ggcaagacct atcatatgac
    16081 cggctggaaa gtgggttatt gtgttgcgcc agcgcccatc agcgccgaaa ttcgcaaggt
    16141 acatcagtat ctgacctttt cggtgaatac cccggcacag ctggcgcttg ctgatatgct
    16201 acgtgcagaa cctgagcatt atcttgcgtt accggacttt tatcgccaga agcgcgatat
    16261 tctggtgaat gctttaaatg aaagccggct ggagatttta ccgtgtgaag gtacatactt
    16321 tttgctggtg gattacagcg cggtttctac cctggatgat gttgagtttt gccagtggct
    16381 gacgcaggag cacggcgtag cggcgattcc gctgtcggtg ttttgcgccg atcccttccc
    16441 acataaactg attcgtctct gttttgccaa gaaggaatcg acgttgctgg cagcagctga
    16501 acgcctgcgc cagctttagc tatttaaccg tccaggcgcg ggaatattga cggtcggcaa
    16561 acaactcttg caggccgtta atttgcttca gtcgcagcac ttcgtcgctg tccattccca
    16621 gctctttgcc aattttatta tcatcccatc ccaactggct aagttcacgg acgatttccg
    16681 acatggcggt gatttgatgg cgtccacgag cgcgattatg acgaatagtc gcggcgatgc
    16741 gttgattacg cgtaccttc
//
LOCUS       DAJTYL010000088        16484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYL010000088 DAJTYL010000000
VERSION     DAJTYL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.088"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..702
                     /gene="ybhA"
                     /locus_tag="OO572_002817"
     CDS             <1..702
                     /gene="ybhA"
                     /locus_tag="OO572_002817"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6661752.1"
                     /translation="IIVTGRHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEAD
                     PMPVNKALQLIEMLNEHHIHGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQ
                     VASLAETTQQVNAVWKFALTHDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNS
                     KGKRLTKWVEAQGWSMENVVAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGD
                     NTTDSIAQFIYSHLI"
     gene            complement(703..1761)
                     /gene="modC"
                     /locus_tag="OO572_002818"
     CDS             complement(703..1761)
                     /gene="modC"
                     /locus_tag="OO572_002818"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6661753.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1764..2453)
                     /gene="modB"
                     /locus_tag="OO572_002819"
     CDS             complement(1764..2453)
                     /gene="modB"
                     /locus_tag="OO572_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6661754.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2453..3226)
                     /gene="modA"
                     /locus_tag="OO572_002820"
     CDS             complement(2453..3226)
                     /gene="modA"
                     /locus_tag="OO572_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6661755.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3393..3542)
                     /gene="acrZ"
                     /locus_tag="OO572_002821"
     CDS             complement(3393..3542)
                     /gene="acrZ"
                     /locus_tag="OO572_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6661756.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3671..4459
                     /gene="modE"
                     /locus_tag="OO572_002822"
     CDS             3671..4459
                     /gene="modE"
                     /locus_tag="OO572_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6661757.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4527..5999
                     /gene="modF"
                     /locus_tag="OO572_002823"
     CDS             4527..5999
                     /gene="modF"
                     /locus_tag="OO572_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6661758.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6260..7276
                     /gene="galE"
                     /locus_tag="OO572_002824"
     CDS             6260..7276
                     /gene="galE"
                     /locus_tag="OO572_002824"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6661759.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7286..8332
                     /gene="galT"
                     /locus_tag="OO572_002825"
     CDS             7286..8332
                     /gene="galT"
                     /locus_tag="OO572_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6661760.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8336..9484
                     /gene="galK"
                     /locus_tag="OO572_002826"
     CDS             8336..9484
                     /gene="galK"
                     /locus_tag="OO572_002826"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6661761.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9478..10518
                     /gene="galM"
                     /locus_tag="OO572_002827"
     CDS             9478..10518
                     /gene="galM"
                     /locus_tag="OO572_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6661762.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10721..11473
                     /gene="gpmA"
                     /locus_tag="OO572_002828"
     CDS             10721..11473
                     /gene="gpmA"
                     /locus_tag="OO572_002828"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6661763.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11640..12692)
                     /gene="aroG"
                     /locus_tag="OO572_002829"
     CDS             complement(11640..12692)
                     /gene="aroG"
                     /locus_tag="OO572_002829"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6661764.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13008..13388
                     /locus_tag="OO572_002830"
     CDS             13008..13388
                     /locus_tag="OO572_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6661765.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13502..14443
                     /gene="zitB"
                     /locus_tag="OO572_002831"
     CDS             13502..14443
                     /gene="zitB"
                     /locus_tag="OO572_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6661766.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14440..15159)
                     /gene="pnuC"
                     /locus_tag="OO572_002832"
     CDS             complement(14440..15159)
                     /gene="pnuC"
                     /locus_tag="OO572_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6661767.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15197..16240)
                     /gene="nadA"
                     /locus_tag="OO572_002833"
     CDS             complement(15197..16240)
                     /gene="nadA"
                     /locus_tag="OO572_002833"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6661768.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4148 a         4343 c         4110 g         3883 t
ORIGIN      
        1 atcatcgtca caggtcgcca tcacgtcgct attcatcctt tttatcaggc gctggcgctg
       61 gatacacctg ctatttgctg taatggcacc tatttgtatg attatcatgc aaaaaccgtg
      121 ctggaagcgg acccaatgcc cgttaataaa gccctgcaac tcattgagat gctgaatgaa
      181 caccacattc acggtctgat gtatgtcgat gatgcaatgg tctatgagca cccgaccggg
      241 catgtcattc gcacgtctaa ctgggcgcaa accctgccgc cggaacagcg tccgactttc
      301 acacaagtcg cttctctggc tgaaacgacg caacaagtta acgccgtatg gaagttcgcc
      361 ctgacgcacg atgacctgcc gcaattgcag cattttggta agcatgtcga acatgaactg
      421 ggactggagt gtgaatggtc ctggcacgat caggttgata ttgcacacgg cggcaacagc
      481 aaaggtaaac gtttgacgaa atgggttgag gcgcaaggtt ggtcgatgga aaacgtcgtg
      541 gcattcggcg ataactttaa tgatatcagt atgctggaag ccgctggtac aggcgtggcg
      601 atgggcaacg ccgatgacgc ggtaaaagcg cgcgccaaca ttgtgattgg tgataacacc
      661 accgacagca ttgcccagtt catttatagc cacctgattt aatcaggcgg ttatcgacac
      721 acttttaatt tgcgcgtaca gccacaggcc aggtttgatc gccagttcat ccctggccca
      781 cgggctgata cgcgcccaca gcgttttacc gccgacttcc agctccactt ccacctggcc
      841 gttgtcgtca taactattaa caacttttgc ccgcaatacg ttacgaatgc tggtttgctg
      901 cggcggttgt aaaaccagag aaacatcgga agcctgaatg cggatacgta gcgcagcttg
      961 cagcggttcg cccagcttat tgacccacaa atgctgatcg cccagcgcca gcgcggtcat
     1021 cgcgtaatgc ggatgatgct caagcaccgt cactttcaga atgctacttt gttgctcttt
     1081 cggcagccac ggattcatca cgctactgcc ccacacttcc tccagcgcgc caaaggcttt
     1141 cacctgaccg ttttccagta ccatcactct gtctgccaga tggaggatct catccagcga
     1201 atggctgaca tacaacatcg gaatgttgat ttcccgtgtc agccgttgca gataaggcaa
     1261 cagttcgcgt ttacgcggaa tatccagtga cgccagcggt tcatccagca acagcaattc
     1321 cggtgctgtc agcaaagccc gaccaatcgc cacgcgctgt ttttcgcctc cggacaggct
     1381 gcctggtaaa cggtcaagca acggttcaat gcctaaaagc gccaccagct tatcgaactg
     1441 atcgaccata cttttcgaca tgccgtagcg cagattgcca cgcactttgt aatgcgggaa
     1501 caaccgcgca tcctgaaaaa catagccaac acgacgcttt tccggcgtca ggcagatacc
     1561 tttttcggca tcatttagta cccgcccatt gaggacaatc cgcccttttt gcgggcgcgt
     1621 cagtccactg atggcgttaa tcagcgaagt ttttccggca ccggagacgc caaagatagc
     1681 agtgatgcca ttggcgggca gcgtttcatt aatggtcagg caatggttgc ccaacgtctg
     1741 ggaaaaattc agctccagca tgattagcgc cccgcccgtt cacggctgat tctggccagc
     1801 cattctgaaa tcaacaggga gatcatcgcc agcgcaatag aaataatgca cagtctcgcc
     1861 gcgccacttt ccccgccggg ggtctggatc agggtataca tggcagaagg aatggttcgc
     1921 gtttcgctag gaatgttcga cacaaaggtg atggttgcac caaactcacc gagagaacga
     1981 gcaaaagcca gtaccgtacc aacaataatt cccggtaagg tcagcggtaa cgtgatagta
     2041 aagaaaacgc gccagcgccc ggcacctagt gttcttgcgg cctgttccag tttgacatca
     2101 accccttcca gcgccagacg aattgcccgc accatcagcg gaaacgacat gacggcggca
     2161 gcgagaaccg cgccgcgcca gctaaaggcg aaggtaatac caaaccagtc atacagacgt
     2221 tcaccgataa atccgcgccg tcccatcgaa actaataata agtaaccgac gaccacgggc
     2281 ggtaacacca gcggtagatg cagtacgctg tcgagcagag ctttgcccgg aaacgtgcaa
     2341 cgcaccagta accaggcaaa aaagatccca aacggcaggc taaacagcac agccagggaa
     2401 gaaactttca ggcttaataa aactgcctgc cattctggat cggtcagtat cattacttgg
     2461 ttgtaaatcc gtaacgttta aagatttccg ctgcctgcgg tcccttcaga taatcataaa
     2521 atgctttcac tgtcgcattg ttatgccctt ccacaacagc aaccggatat tccacttttt
     2581 tatgtgaatc ttccgggaag gtggcaacca cttttacccc tttgctggca actgcgtcag
     2641 aaccgtagac aatgcccaga ggcgcttcgt tacgttcgac cagcgccagc gccccacgaa
     2701 catcttccgc tggggccagt ttcggagaga gcgtatccca tgcgcccagt ttttgcagtg
     2761 cttcttttgc ataaatgcca gcgggaacat gttccggatc gccaaccgcc aggcgaccgc
     2821 cattcagcag cgaagtccag ttagttttgc tgtcgatggt gaaatctttc tgctcgctgg
     2881 ctttcggtgc tacaacgacc aggctattgc cgagcagtgt ctgacgcgta gcagtatcga
     2941 tcgctttttt atcaaccgca taatccatcc atttctgatc ggcagaaata aacagatccg
     3001 caggggcacc cgcttcaatc tgacgggcga gagtagatga cgaagcgaaa gaagaaacca
     3061 catccacgcc tttctctttt ttatactgcg tagcaatgtc ctgcattgcg ttagtcagtg
     3121 atgcggcggc gaacaccgtg attttccctt catctgccag tgcattgcca gcaactgcga
     3181 aagagagtgc cgccccggca aacaagttca accatttacg agccattggt aaccccttaa
     3241 tgtaacgtta tgtaggcgac aatataacga ctaactcgga attttcccag cagttattgc
     3301 taacctttgt gaggtagata agaaaaaata tcggcaggaa aagcaggaag ttgagaaaaa
     3361 gaaaatgccc gactaagcgg gcactcaggg aatcaatgat tttgtccggg ctggtctttt
     3421 ttaccaacac cagaaaagat gttgaatact tcaccaagac cgtaaatcag acccaggatg
     3481 atggccatca cgacaggtac catgattacg gcgaatacca gactttttaa taactctaac
     3541 atggtcaact ccagatatag tcatgagact attctaaccg ctaagcacag aaaagcactc
     3601 cccttttgtg cggtcagctt tgcgtggcgt tcgttttccg tcacaataag acttttgcca
     3661 ggacattgtt atgcaggccg aaatccttct cacccttaag ctccaacaaa aattattcgc
     3721 cgacccgcgc cgcatttcgc tactaaaaca cattgcgctt tccggttcca ttagccaggg
     3781 agcgaaagat gccggtatca gctataaaag cgcctgggat gccattaacg agatgaatca
     3841 gttaagtgag catattctgg tcgagcgcgc aacaggcggt aaaggtggcg gaggcgcagt
     3901 actgacccgc tatggtcagc gactgattca gctctatgac ttactggcgc aaatccagca
     3961 aaaagccttt gatgtgttaa gtgacgatga cgccctgccg ctgaacagcc tgctggccgc
     4021 gatctcacgt ttttcactgc aaaccagcgc ccgtaaccag tggttcggta ccatcaccgc
     4081 ccgcgatcat gatgacgttc aacagcatgt tgatgtctta ctggctgacg gaaaaacacg
     4141 cctgaaagtc gcaattaccg cacaaagcgg cgcacgtctg gggctggatg aaggcaaaga
     4201 agtgttgata ttgctaaaag cgccgtgggt aggcattact caggacgaag cggtcgcaca
     4261 aaacgccgac aaccaattac cgggtattat tagtcatatt gagcgcggcg cagagcagtg
     4321 cgaagtatta atggcgctac ccgacgggca aacactgtgc gccacagtgc cggtaaatga
     4381 agcgacttct cttcagcaag ggcagaatgt cacggcctac tttaatgccg acagcgtgat
     4441 tatcgccacg ctgtgctaag cgtgttgaca atttgttatg aaacacgtat ccctgtcagt
     4501 aatcgctgca caaaatggga tataaaatgt catcgttgca aattttgcaa ggcacgtttc
     4561 gtcttagcga cacaaaaacg ctgcaattgc ctcagctaac gttaaacgcg ggtgatagtt
     4621 gggcgtttgt cggttcgaat ggaagtggaa agtcggcgct ggcccgcgcg ctggcggggg
     4681 aacttccgct tttgaaaggt gaacggcaaa gccagttttc ccacatcact cgtctctcct
     4741 tcgagcaatt gcaaaagctc gtcagcgacg aatggcaacg gaataacacc gatatgctcg
     4801 gccctagcga agattacacc ggacgcacta cggctgagat tattcaggat gaagtaaagg
     4861 atgcaccgcg ttgcatgcaa ctggcgcagc agttcggtat taccgccctc ctcgaccgac
     4921 gctttaaata cctttccact ggcgagacgc gaaaaaccct gctgtgtcag gcgctgatgt
     4981 cggagcctga cttgttgatt cttgatgagc cgttcgatgg cctggatgtt gcctcacgtc
     5041 agcagctggc tgagcgactc gcctcgttac atcagtccgg tattactttg gtactggtgc
     5101 tcaatcgctt cgataagatc ccggaatttg tccagtttgc tggcgtgctg gcggattgta
     5161 cgttagcgga aactggcgct aaagaggaac tgctccagca agcactcgtc gcgcaacttg
     5221 cgcatagtga acagcttgaa ggtgtgcaac tgccggagcc ggatgaacct tcagcacgtc
     5281 acgccttacc cgccaacgaa tcgcgcattg tgctgaacaa tggcgtggtt tcttataatg
     5341 atcgccccat tcttaataac cttagctggc aggtgaatcc aggcgaacac tggcaaattg
     5401 tcgggccaaa tggcgcggga aaatcgacgt tattaagcct gattactggc gatcatccgc
     5461 aaggttacag caacgatttg acgcttttcg gccgacgtcg cggcagcagc gaaaccatct
     5521 gggacatcaa aaagcatatc ggttacgtca gcagtagttt gcatctggat taccgggtca
     5581 gcactaccgt gcgtaatgtg attctttctg gctattttga ttcgattggc atttatcagg
     5641 tcgtttcgga tcgccagcaa aaactggtgc agcagtggct ggatattctc ggcattgata
     5701 aacacacggc tgacgctccg ttccatagtc tttcctgggg acagcagcgt ttggcgctga
     5761 tcgtccgcgc actggtgaaa catccgacgt tgcttattct cgatgaacca ctacaggggc
     5821 ttgatccgct caatcgccaa cttatccgcc gttttgttga tgtgctgatt agcgaaggtg
     5881 aaacgcaatt gttgtttgtt tcgcaccacg ctgaagatgc gcctgcctgt attacccatc
     5941 gccttgagtt cgtgccggac ggtggactct atcgctatgt gctgacaaaa atatattgag
     6001 tcggtagtgc tgaccttgcc ggaggcggcc taagcgcact ctccggccaa cggttcgacg
     6061 catgcaggca tgaaaccgct tcttttttca gataaaaagc gcaatcattc ataaaccctc
     6121 tgttttataa tcacttaatc gcgcataaaa aacggctaaa ttcttgtgta aacgattcca
     6181 ctaatttatt ccatgtcaca cttttcgcat ctttgttatg ctatggttat ttcataccat
     6241 aagcctaatg gagcgaatta tgagagttct ggttaccggt ggtagcggtt acattggaag
     6301 tcatacctgc gtgcaattac tgcaaaacgg tcatgatgtc atcattcttg ataacctctg
     6361 taacagtaag cgcagcgtac tgcctgttat cgagcgttta ggcggcaaac atccaacgtt
     6421 tgttgaaggc gatattcgta acgaagcgtt gatgaccgag atcctgcacg atcacgctat
     6481 cgacaccgtg atccacttcg ccgggctgaa agccgtcgcc gaatcggtac aaaaaccgct
     6541 ggaatattac gacaacaatg tcaacggcac tctgcgcctg attagcgcca tgcgcgccgc
     6601 taacgtcaaa aactttattt ttagctcctc cgccaccgtt tatggcgatc agccccaaat
     6661 tccatacgtt gaaagcttcc cgaccggcac accgcaaagc ccttatggca aaagcaaatt
     6721 gatggtggaa cagatcctca ccgacctgca aaaagcccag ccggactgga gcattgccct
     6781 gctgcgctac ttcaacccgg ttggcgcgca tccgtcgggc gatatgggcg aagatccgca
     6841 aggcattccg aataacctga tgccatacat cgcccaagtt gctgtaggcc gtcgcgactc
     6901 gctggcgatt tttggtaacg attatccgac cgaagatggt actggcgtac gcgattacat
     6961 ccacgtaatg gatctggcgg acggtcacgt cgtggcgatg gaaaaactgg cgaacaagcc
     7021 aggcgtacac atctacaacc tcggtgctgg cgtaggcagc agcgtgctgg acgtggttaa
     7081 tgccttcagc aaagcctgcg gcaaaccggt taactatcat tttgcaccgc gtcgcgaagg
     7141 cgaccttccg gcctactggg cggacgccag caaagccgac cgtgaactga actggcgcgt
     7201 aacgcgcaca ctcgatgaaa tggcgcagga cacctggcac tggcagtcac gccatccaca
     7261 gggatatccc gattaaggaa cgaccatgac gcaatttaat cccgttgatc atccacatcg
     7321 ccgctacaac ccgctcaccg ggcaatggat tctggtttca ccgcaccgcg ctaagcgccc
     7381 ctggcagggg gcgcaggaaa cgccagccaa acaggtgtta cctgcgcacg atccagattg
     7441 cttcctctgc gcaggtaatg tgcgggtgac aggcgataaa aaccccgatt acaccgggac
     7501 ttacgttttc actaatgact ttgcggcctt gatgtctgac acgccagatg cgccagaaag
     7561 caacgatcct ttgatgcgtt gccagagcgc gcgcggtacc agccgggtga tctgcttttc
     7621 accggatcac agtaaaacgt tgccagaact gagcgttgcg gcattgacgg aaatcgtcaa
     7681 aacctggcag gagcaaaccg cagagctggg aaaaacgtac ccgtgggtgc aggtctttga
     7741 aaacaaaggt gcggcgatgg gctgctctaa cccgcatccg cacggacaga tttgggcaaa
     7801 tagcttcctg cctaacgaag ctgagtgcga agaccgcctg caaaaagaat attttgccga
     7861 acagaaatca ccaatgctgg tggattatgt tcagcgcgag ctggcagacg gtagccgtac
     7921 cgttgtcgaa accgaacact ggttagccgt tgtgccttac tgggctgcct ggccgttcga
     7981 aacgctactg ctgcccaaag cccacgtttt gcggatcacc gatttgaccg acgcccagcg
     8041 cagcgatttg gcgctggcgt tgaaaaagct gaccagtcgt tatgacaacc tcttccagtg
     8101 ctccttcccc tactctatgg gctggcacgg cgcaccattt aatggcgaag agaatcaaca
     8161 ctggcagctg cacgcgcact tttatccgcc tctgctgcgc tccgccaccg tacgtaaatt
     8221 tatggttggt tatgaaatgc tggcagagac ccagcgagac ctgaccgcag aacaggcagc
     8281 agagcgtttg cgcgcagtca gcgatatcca ttttcgcgaa tccggagtgt aagaaatgag
     8341 tctgaaagaa aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcacac
     8401 cattcaggcg cctggccgcg tgaatttgat tggtgaacac accgactaca acgacggttt
     8461 cgttctgccc tgcgcgattg attatcaaac cgtgatcagc tgtgcaccac gcgatgaccg
     8521 taaagttcgc gtgatggcag ccgattatga aaatcagctc gacgagtttt ccctcaatgc
     8581 gcccattgtc gcgcatgaaa actatcaatg ggcgaactac gttcgtggcg tggtgaaaca
     8641 tctgcaactg cgtaacaaca gcttcggcgg tgtggacatg gtgatcagcg gcaatgtgcc
     8701 gcagggtgcc gggttaagtt cttccgcttc actggaagtc gcggtcggaa ccgtattgca
     8761 gcagctttat catctgccgc tggacggcgc acaaatcgcg cttaacggtc aggaagcaga
     8821 aaaccagttt gttggctgta actgcgggat catggatcag ctaatttccg cactcggcaa
     8881 gaaagatcat gccttgctga ttgactgccg ctcactgggg actaaagcag tttccatgcc
     8941 gaaaggtgtg gctgtcgtca tcatcaacag taacttcaaa cgtaccctgg ttggcagcga
     9001 atacaacacc cgtcgtgaac agtgcgaaac cggtgcgcgt ttcttccagc agccagctct
     9061 gcgcgatgtc accattgaag agttcaacgc tgttgcacat gagctggacc caatcgtggc
     9121 gaaacgcgtg cgtcatatcc tgactgaaaa cgcccgcacc gttgaagctg ccagcgcgct
     9181 ggagcaaggc gacctgaaac gtatgggcga gttgatggcc gagtctcatg cctctatgcg
     9241 cgatgatttc gaaatcaccg tgccgcaaat tgacactctg gtagaaatcg tcaaagctgt
     9301 gattggcgac aaaggtggcg tacgcatgac cggcggcgga tttggcggct gtatcgtcgc
     9361 gctgatcccg gaagagctgg tgcctgccgt acagcaagct gtcgctgaac aatatgaagc
     9421 aaaaacaggt attaaagaga ctttttacgt ttgtaaacca tcacaaggag caggacagtg
     9481 ctgaacgaaa ctcccgcact ggcacccgat ggtcagccgt accgactgtt aactttgcgt
     9541 aacaacgcag ggatggtagt cacgctgatg gactggggtg cgactttact ttccgcccgt
     9601 attccgcttt ccgatggcag cgtccgcgag gcgctgctcg gctgtgccag tccggaatgc
     9661 tatcaggatc aggctgcgtt tctgggggcc tctattggtc gttatgccaa ccgcatcgcc
     9721 aatagtcgct atacctttga cggtgaaacc gtgacgcttt cgccaagcca gggcgttaac
     9781 cagctgcacg gcggcccgga agggttcgac aaacgtcgct ggcagattgt gaaccagaac
     9841 gatcgtcagg tgctgtttgc cctgagttca gatgatggcg atcagggctt cccgggtaat
     9901 ctcggcgcga cggtgcaata tcgtctgacc gacgataacc gtatctccat tacttatcgc
     9961 gccacagttg ataaaccttg cccggtgaat atgactaatc acgtctattt caatcttgac
    10021 ggcgagcagt ctgacgtgcg caatcacaag ttgcagattc tggcggacga atatcttccg
    10081 gttgatgaag gcggcattcc gcacgacggc ttgaaatctg tcgccggaac gtcttttgat
    10141 ttccgcagcg ccaaaatcat cgccagtgag tttcttgccg acgacgatca gcgcaaagtg
    10201 aaaggttacg atcacgcatt cttgttacag gccaaaggcg atggcaagaa agtggcggcg
    10261 catgtctggt cagcagatga aaaattgcag ctgaaggtct acaccaccgc tccggctctt
    10321 caattctact ccggcaactt cctcggcggc acaccgtcgc ggggaaccga accttacgcc
    10381 gactggcaag ggctggcgtt ggaaagcgag tttctgccgg acagcccgaa ccaccctgaa
    10441 tggccgcaac cggactgctt cctgcgtcct ggcgaagagt attccagcct gacggaatat
    10501 cagtttattg ctgagtaatg ttaagccctc gtcatgaggg ctttatcttc atattgttca
    10561 aatcaccagc aaacaccgac atatttgcaa ctcaatattc acaacaacct tacactgcgc
    10621 cactattttc gctatggtta tgcgtaagca ttgctgttgc ttcgtcacgg caatataatg
    10681 agaattatta tcattaaaag atgatttgag gagtaagcat atggctgtaa ctaagctggt
    10741 tctggttcgt catggcgaaa gtcagtggaa caaagaaaac cgtttcaccg gttggtacga
    10801 cgtggatctg tctgagaaag gcgtaagcga agcaaaagca gcaggtaagc tgctgaaaga
    10861 ggaaggttac agctttgact ttgcttacac ttctgtgctg aaacgcgcta tccataccct
    10921 gtggaatgtg ctggacgaac tggatcaggc atggctgccc gttgagaaat cctggaaact
    10981 gaacgaacgt cactacggtg cgttgcaggg tctgaacaaa gcggaaactg ctgaaaagta
    11041 tggcgacgag caggtgaaac agtggcgtcg tggttttgca gtgactccgc cggaactgac
    11101 taaagatgat gagcgttatc cgggtcacga tccgcgttac gcgaaactga gcgagaaaga
    11161 attgccgctg acggaaagcc tggcgctgac cattgaccgc gtgatccctt actggaatga
    11221 aaccattctg ccgcgtatga agagcggtga gcgcgtaatc atcgccgcac acggtaactc
    11281 cctgcgtgcg ctggtgaaat atcttgataa catgagcgaa gaagagattc ttgagctgaa
    11341 tatcccgact ggcgtgccgc tggtgtatga gttcgacgag aatttcaaac cgctgaaacg
    11401 ctattatctg ggtaatgctg acgagatcgc ggcgaaagca gcagccgttg cgaaccaggg
    11461 taaagcgaag taaacgtcat tcgtttaaaa aatgagaaag ccgactgcaa gtgagtcggc
    11521 ttttttgttt gtcgttgcta acaatggagc acattgcctg atgcgacgct gcgcgtctta
    11581 tcaggcctgt ggtgattcat cggatacgcc actctgacgg cgcatccgac aattaaacct
    11641 tacccgcgac gcgcttttac tgcattcacc agttgacgta acagagcatc ggtatcttcc
    11701 cagccaatgc aggcatcggt gatgctctta ccgtaggcca gcggctcccc gctatcgagg
    11761 ctctgattgc cttccaccag atggctttcc accatcacgc caataatggc cttttcgcca
    11821 ccggcaatct gctggcaaac gtcagcacaa acatccatct gctttttgaa ttgtttggac
    11881 gaattagcat ggctgaaatc gatcatcacc tgagctggca gacctgcttt gttcagccct
    11941 tctttcactt cagcaacgtg cttcgcgctg tagttaggct ctttaccgcc gcgcagaatg
    12001 atatggcaat cgccgttacc actggtattc acaatcgccg aatgccccca tttagttacg
    12061 gacaggaagc agtgcggcgc accggcggca ttaatggcat cgatagccac tttaatcgta
    12121 ccgtctgtgc catttttaaa acctaccgga caagaaagac cagacgccag ttcgcggtgc
    12181 acctgcgatt ccgtggtacg tgcaccaatt gcgccccagc tcatcaggtc agcgagatac
    12241 tgaggagtga tcatatcgag aaactcaccc gccgctggca gaccgctgtc gttaatatca
    12301 agcagcaatt tacgggctat acgcagaccg tcgttgatct ggaagctgtt atccatatgc
    12361 ggatcgttaa tcaacccttt ccagcccacc gtggtacgcg gcttttcaaa atagacgcgc
    12421 attacgattt ccagctcatc tttcagctct tcacgcagcg ccagcaagcg agtggcatac
    12481 tctttagccg cgacaggatc atgaattgag cacgggccaa tcaccaccaa caggcgatca
    12541 tcattacctt tcaggatctt atggatcgct tttcgggcat gggcaaccgt attcgcggca
    12601 ttttcagttg cggggaattt ttccagcaat gcgacaggag gaagtaactc tttgatttct
    12661 ttgatgcgta aatcgtcgtt ctgataattc atgtctgttc cagtgttgcc atacttatct
    12721 tagtgaatgc aatacttcca atctatatct tccgtcagaa tgtgtaaacg gggttttaca
    12781 ctatgaacgg attaatcctg gaattagcaa gaaaaacgcc agattgtcgc gaaaaacgag
    12841 atctctccta caatttctaa ctgtaactcc tttgtttgtt aattatttca agattctctg
    12901 ctgcgtttca taaactggct gaaatcttaa accaatgcct tatattcacc tgcaaatgca
    12961 ctgttggaag aggttatccg acataacgac cataacagga gcatcctatg aaaatgacaa
    13021 aactggccac attatttctg actgccactc taagccttgc cagcggtgcc gcactggctg
    13081 ccgatagcgg agcgcaaact aataacggcc aggcaaacgc cgcagctgat gcgggccagg
    13141 tagcccctga cgcccgtgaa aatgtcgcgc caaataacgt cgacaataac ggggtaaata
    13201 ccggttctgg cggcacaatg ctgcattcgg atggttcttc aatgaacaat gacggaatga
    13261 ccaaagatga agagcacaag aacaccatgt gcaaagatgg tcgatgtccg gatatcaata
    13321 aaaaggtaca aaccggtgat ggcatcaata atgatgtcga taccaaaacc gacggtacca
    13381 cgcagtaacc ctgcaaaata gccaataatg ggagagcgga gtctctccct tttaattttt
    13441 cacttctaaa agcagtatga tgtcataaag ttaagtgaga ataattaagg gatgactcgt
    13501 catggcgcac tcacactcac acacgtcctc acacctgcca gaagataata atgctcgtcg
    13561 cttgttgtat gctttcggcg tgactgccgg gtttatgctg gtagaagtcg ttggtggttt
    13621 tctttctggt tctctggcat tgctggccga tgcgggtcat atgttgaccg atactgccgc
    13681 cctgcttttc gccctactcg ccgtgcaatt ttcacgtcgt cctcccacca ttcgccacac
    13741 tttcggttgg ctaagactga ccacgctcgc ggcgtttgtg aatgccatcg ccctcgtggt
    13801 aattaccatt ttgattgtct gggaggcgat agaacgcttc cgcacgccgc gtccggtcga
    13861 gggaggcatg atgatggcaa ttgccgtggc tgggttgctg gcaaatatac tttctttctg
    13921 gttacttcat cacggcagcg aagagaaaaa cctcaacgta cgagcagcgg cactgcatgt
    13981 gctcggggac ctgctgggtt cggttggggc tattattgcc gcgttgatta ttatctggac
    14041 tggctggaca cctgctgacc ccattctctc gatactggtg tcgcttctgg ttctgcgtag
    14101 cgcgtggcga ctgttgaaag atagcgtgaa tgaattactt gaaggtgcac cggtatcgct
    14161 ggatatcgct gaactgaagc gtcgtatgtg ccgggaaatc ccggaagtcc gcaatgtgca
    14221 ccatgtacat gtatggatgg taggcgagaa gccggtgatg acgttgcatg tgcaggtgat
    14281 cccgccacac gatcacgatg ccttgttgga tcagatccaa cactacctga tggatcacta
    14341 tcagattgag cacaccacca ttcagatgga atatcaacct tgtcataggc cagactgcca
    14401 cctgaatgaa ggggtgtctg gccattcaca tcatcaccat taatgggaca gcgcgcgtga
    14461 gcctctttca cgtgcgctgt tgatccacat ccggctgccg ttgagcgcaa taaaggtcag
    14521 gatgatgtac tccagagaca tggcgtaaac gccctgaagt gcaaaaataa cgacgctaat
    14581 cacgttaata atcacccaca acagccagtt ttccacatac ttacgcgtca tcagaatcat
    14641 tgccacgata gataacacca tcatgcatga atcccagaac gggaaagcgt ccggttgcag
    14701 ttcaggcatc acaacctgta atcctaatgc ttgcatgatc atgactgcca cgcgggtcaa
    14761 aaatgcaaac accggattga taaataccgt catcagacca atcgaaacaa cgcaaaccgc
    14821 caaccagctg agtgccttcg gcaatggcaa ccagcgaatt ttcaactccg cctcgttctg
    14881 actggtttgt cgcgaccacg cataccagcc gtaaatattc gcggcaaaga aaaacacctg
    14941 taatagcagg ctggcataca gctgaatctg aaagaaaata atgccaaaca aggtaacgtt
    15001 gatcaggcca aagaagtagt tgctgatctt ctccagactg gcaaggccaa tacacagcaa
    15061 cccggcgatc gtgcctaccg cttcgatcca tgagagatca taaccgcccg cccctattgg
    15121 tatatgtacc aggatattct gcacactaaa aaaatccatc tttcccccaa agcgtaacgt
    15181 tacgccttat tattcgttat ccacgtagtg tagccgcaaa atccagcata cgattgagcg
    15241 gcaccagcgc cctctctcgc agcctctcat cgacatgaac ctcgtgatta cttccttcct
    15301 gttctaatgc ctctgcgata gcctgaaggt cattcatggc catccacgga caatgcgcgc
    15361 agctgcggca ggttgcgccc tcacccgcgg ttggtgcttc cagtaactct ttatctggca
    15421 ccgcctgctg cattttgtag aaaatacccc gatcggttgc cacaataagc ctctgatgag
    15481 gcaatgcttt tgcagcagcg atcagttgac tggtggaacc gaccgcatcc gccatatcga
    15541 caatagcttg tggtgattct ggatgcacca gtatggcagc atccgggtat tcttcttgca
    15601 agcgggttaa cgcctgagtc ttaaattcat catgcacaat gcaggcaccc tgccagcata
    15661 aaatgtctcc acccgtctgt ttttgcacgt aacgccccag atgtttgtcg ggtgcccaga
    15721 tgattttttc acccaaacta tcaagatgat caataagttc gacggcaatg cttgaagtta
    15781 ccacccaatc tgcgcgcgct tttaccgcag cagaagtgtt ggcgtagacg acgacagtac
    15841 gatcgggatg ggcatcgtaa aatgcgttaa attcttcaac agggcagccg agatccagtg
    15901 aacactcaac ctgaagtgtc ggcatcagaa ttgttttttc cggactgaga attttggcgg
    15961 tttctcccat aaatctcacc ccagcgacta acaaagtaga agcgggatgc tttgcaccga
    16021 agcgcgccat ttccagagaa tcagaaatac agccaccggt ttcttctgcc agttgttgaa
    16081 tttcgggatc ggtatagtag tgggcaacca tcaccgcatt acgttctttt agcagacgtt
    16141 ttatcttctc gcggtaatac gctttttcat caatgcttaa cggcgtcggc ttcgggggga
    16201 aaggataaat cgccgtgtct ggatcaaaca ttacgctcat cttaccatct cgttttacag
    16261 gcttaacgtt aaaaccgaca ttagcgtaac attcgctgtt atcggtgtaa tttgttttat
    16321 atgctaaaca agatagtcga atggtgggga aaagtcactc gaattacgcc taattccacc
    16381 gacgtagagt tgcttgcaca gtaagctctg acgatgtcat caggtttaca aagtgaaatt
    16441 gaggatgcct gaatagcaaa aaagcgcctt tagggcgctt tttt
//
LOCUS       DAJTYL010000089        16469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYL010000089 DAJTYL010000000
VERSION     DAJTYL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.089"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(36..2477)
                     /gene="rnr"
                     /locus_tag="OO572_002834"
     CDS             complement(36..2477)
                     /gene="rnr"
                     /locus_tag="OO572_002834"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6661769.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2516..2941)
                     /gene="nsrR"
                     /locus_tag="OO572_002835"
     CDS             complement(2516..2941)
                     /gene="nsrR"
                     /locus_tag="OO572_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6661770.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3146..4444)
                     /gene="purA"
                     /locus_tag="OO572_002836"
     CDS             complement(3146..4444)
                     /gene="purA"
                     /locus_tag="OO572_002836"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6661771.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4548..4745)
                     /locus_tag="OO572_002837"
     CDS             complement(4548..4745)
                     /locus_tag="OO572_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6661772.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4827..5831)
                     /gene="hflC"
                     /locus_tag="OO572_002838"
     CDS             complement(4827..5831)
                     /gene="hflC"
                     /locus_tag="OO572_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6661773.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5834..7093)
                     /gene="hflK"
                     /locus_tag="OO572_002839"
     CDS             complement(5834..7093)
                     /gene="hflK"
                     /locus_tag="OO572_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6661774.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7179..8459)
                     /gene="hflX"
                     /locus_tag="OO572_002840"
     CDS             complement(7179..8459)
                     /gene="hflX"
                     /locus_tag="OO572_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6661775.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8535..8843)
                     /gene="hfq"
                     /locus_tag="OO572_002841"
     CDS             complement(8535..8843)
                     /gene="hfq"
                     /locus_tag="OO572_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6661776.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8929..9879)
                     /gene="miaA"
                     /locus_tag="OO572_002842"
     CDS             complement(8929..9879)
                     /gene="miaA"
                     /locus_tag="OO572_002842"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6661777.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9872..11719)
                     /gene="mutL"
                     /locus_tag="OO572_002843"
     CDS             complement(9872..11719)
                     /gene="mutL"
                     /locus_tag="OO572_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6661778.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11729..13066)
                     /gene="amiB"
                     /locus_tag="OO572_002844"
     CDS             complement(11729..13066)
                     /gene="amiB"
                     /locus_tag="OO572_002844"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6661779.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13085..13546)
                     /gene="tsaE"
                     /locus_tag="OO572_002845"
     CDS             complement(13085..13546)
                     /gene="tsaE"
                     /locus_tag="OO572_002845"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6661780.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13518..15065)
                     /gene="nnr"
                     /locus_tag="OO572_002846"
     CDS             complement(13518..15065)
                     /gene="nnr"
                     /locus_tag="OO572_002846"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6661781.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15064..16203
                     /gene="queG"
                     /locus_tag="OO572_002847"
     CDS             15064..16203
                     /gene="queG"
                     /locus_tag="OO572_002847"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6661782.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16186..16239)
                     /locus_tag="OO572_002848"
     CDS             complement(16186..16239)
                     /locus_tag="OO572_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661783.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3738 a         4703 c         4134 g         3894 t
ORIGIN      
        1 gcaatctaac cctcttcttt taaagagggt attgatcact ctgccacttt tttcttcgcc
       61 gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct
      121 tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg
      181 tctggctcaa agtttacctt tttaccgacc tgacaacgct tgccgccttt tttgcctgca
      241 tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg
      301 gagatcagac taaagtcgat tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc
      361 acgcgatcgc ccaggcgata agtctggccg ctggattccc ccatcaggcg ttgccctacc
      421 tggtcaaagc gatagtagtc attgtccagc gaagagacat ggaccagacc atcaatgaac
      481 aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc tggaaattac gcctttaaag
      541 acgttaccta cctggtcgag catgaagtca cacttcagcc agtcagcgac atcgcgcgtt
      601 gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa catctcttcc
      661 atcgaataat ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc
      721 agcagatatt taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta
      781 aagtgcgcat aggactgcaa tgccagaccg aagtgaccac ggttttctgg atcgtaaatc
      841 gcctgtttca tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg acggtcggca
      901 accgactcca gcagttccgc gtagtcacgc ggttccggct tgttaccacc cggcagctcc
      961 agccccagct ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg
     1021 tgaatacgga acagtgccgg ttctttcgct ttctcaacga aacgcgccgc cgagatattc
     1081 gccagaatca tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata
     1141 cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc aaatgagatc
     1201 ccaccgcgtt cttcacgggc tttatccagc actttataga ggttatgcaa ctcttcgaga
     1261 tgcttaacca gcggggcgta ctgctcgcgc agatcctgat cgccctgcag aatatgccag
     1321 actttggtgt aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc
     1381 gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca tacacaggcg gtctacctgc
     1441 gggttgagcg aacacaggcc gttagagagc acttccggca gcatcgggat aacctgcgaa
     1501 gggaagtaca ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt tggcggacgc
     1561 acatagtagc tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc gcgttttttc
     1621 tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt
     1681 aaatcgcgca aatcgacacg gcccgctttt gcttcttccg gcacttcttc tttcagcccc
     1741 gcaacctgtt gctcaacagc ctgcggccag atatacggaa tttcatgggt acgcagagcg
     1801 atatcaaccg ccatgccggt gcccatattg tcgcccagca cttcgacgat tttacccacc
     1861 gctttggtgc ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa gcccatccgc
     1921 gcgcccatga tctgatcggg cgggattaag atatcgaagc tcagacggct atcgtcagga
     1981 accacaaagc cgacgcccgc ttcggtaaag tagcgaccaa caatctggct ggtttttggc
     2041 accagtacgc ggacaatacg cgcttcacga cgacctttac ggtcagcacc cagcggctgc
     2101 gccagcacct gatcgccatg aatgcaggtt ttcatctgct cgctggagag atacaaatca
     2161 tctttacgcc cttcaacccg cagaaagccg tagccatcac ggtgaccaat aacggtacct
     2221 ttcaccaggt cgaggcgttc cggcagcgca tagcactgac gacgagtgaa gaccagttga
     2281 ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt caagctgctc ttcgccttca
     2341 atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt
     2401 tcgaggataa attcccgact agggatggga ttcgcgtatt tttcagcttc gcgttcctgg
     2461 aaaggatctt gtgacatctc ggttcctccg ttgtcatctc tgatgaagat tttcgtcact
     2521 ccaccagcaa taatttataa agcggttgat tctcttcaac caaatcggca agcgtgtagt
     2581 tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg
     2641 caggtgtaat gtggcaaaac tcactgctgc aattcaccag cgataagggc tccagctcgc
     2701 gcaccacatc accaatacgt atcgcactcg ccggtttacc caggcgaatg ccgccatttt
     2761 ttccacgaac agcagtcacg tagccggcac gactaagttg attgattatt ttgaccatat
     2821 gattacggga gacgccgtag acgtcagtca cttcagaaat actggtcatc cgcccttctg
     2881 gcaatgacgc catgtagatc agcgcacgta atccgtaatc agtgaaactc gttaactgca
     2941 catcaacctc aaaagggaaa tcgggaaaaa atacatttat attgatgata aaccagccac
     3001 aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt
     3061 gtcggatagc gtaatggctt aaccgacaca gcaatgtatc acactgttag cccggcaggc
     3121 aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg gtcgcgcaga atcatggttt
     3181 cagtacgatc cggaccggta gagatgatat cgatcggcac accggtcagc tcttcaatac
     3241 gcttgatgta gttcagcgcc gcctgcggca ggccgctacg atctttcacg ccgaaagtgg
     3301 attcagacca gcccggcatg gtttcgtaaa tcggctctac acctttccag tcgtcagctg
     3361 ccagcggagt ggtagtcact tcgcgaccat ccggcatacg gtaagccacg cagagtttaa
     3421 cctctttcag gccatccaga acgtccagtt tagtcaggca gaagccagac agggagttca
     3481 gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc ggtacgacga cgacgacccg
     3541 tagttgcacc gaattcgtta ccctgcttgc agaggaactc gccagtttca tcaaacagtt
     3601 cggccgggaa aggacctgca cctacacgag ttgagtaagc tttgaggata cccagaacgt
     3661 aatcaacata acgcgggccc aggccggaac cggtcgccac gccaccagca gtggtgttgg
     3721 aagaagttac gtacggataa gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt
     3781 cgaacatgac gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa acgtcaacca
     3841 ccatagaagt caggatgtcg gcaacagcca tcgtatcatc cagaactttc tggtaatcaa
     3901 ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg atattccatc acttctttca
     3961 gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcca
     4021 ctttatcttc ataagcaggc ccgataccac gaccggtggt gccgatcgct ttcgcgccac
     4081 gcgctttctc acgcgcgtta tccagagcaa cgtgataatc aaggatcagc ggacatgctt
     4141 cagacagcag cagacgctca cgaacgggga tgccacggtc ttccagttct ttcatctctt
     4201 tcatcagcgc ggccggagac agcacaacac cgttaccgat gatgctggtt acattctcgc
     4261 ggagaatacc tgatggaata agatggagaa cggttttttc accgttgatt acgagagtat
     4321 ggcctgcgtt gtgaccgccc tggtagcgta caacatattt agcccgttca gtcagaagat
     4381 cgacgatctt acctttacct tcgtcacccc attgggtgcc cagtacgacg acgttgttac
     4441 ccatttttca aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt ttcagagtta
     4501 cagcactttt tgcactcaaa aatgacctcg atcagtctgt tttttgttca gccaatcgtt
     4561 ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt
     4621 aaaatattat cgggcaaatt ggtcatcgca gagatcatct tcttccatgc cttcgggtaa
     4681 agcatcggcc ctaaaccttc cagtaccaaa accagggcaa gcgccagcca gattgtcgaa
     4741 ttcattcatt atccttatag aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt
     4801 tattgacctg taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag
     4861 cggaagaaat cgctatccgg gctcatgacc atcacgtcct gattgccaga gaagctgttc
     4921 tcataagcac gcaggctacg gatgaatgcg tagaagtccg gatctttact gaatgcatca
     4981 gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca taatgcggcc ctgacgctca
     5041 gcttctgcca gcgttctggt cacttcatag tcggcagtcg cgcgcagttt ttccgcttct
     5101 tcctgacctt gtgaacggtg acgacgcgct accgcttcac gctcggcgcg catacggttg
     5161 tagatcgctt cagacacttc ggtcggcagg ttgatctgct tgatacgcac atcgacaact
     5221 tcaataccca gcgccgccat actgttcggg ttgatgaccg gaactttgcc cttcgtctca
     5281 gccgttacgc gctctgccgc ttcggcaatg gcgttatctg ctgccggggt agtaacttca
     5341 tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt cagacgacca
     5401 cgggaatcgg tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg cagacggtca
     5461 gagaacttac gtttcaacag cacttccgct tgcgaaatgt cgccaccacc cgttgccagg
     5521 tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag agtcgacgat caggtctttc
     5581 ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc
     5641 attttcaccg tttcaatgaa cggtatcttg aaatgcagac caggctcata aaccaaaggt
     5701 ttgttgtcat cgtcacgcag taccttacca aaacgcagcg taataccgcg ctcaccttct
     5761 ttgacgacaa agacagacat gtaaagcact accagcacga tgatgataat cgcgataact
     5821 gacttacgca tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc
     5881 gttggtccat aatatcgccc tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg
     5941 aggaagaggc tggcggcaga cgcagcagat tgctggcacc gttatcgctc ttcgccgcag
     6001 gggcgttacc acctttcagc atctggtcta acggcagaac catcaggttg ccacctttat
     6061 cgttaaccag cactttgcgg gtgttaccca acactttttc catcgtctcg atatacagac
     6121 gctcgcgagt aatttccggc gcggctttat attccggcag aagtttagca aagcgcgcca
     6181 cttcaccctg agcttccagg atggtctggg ccttgtacgc acgcgcctct tcgaggatac
     6241 gttgcgcctg accgttcgca cgcggctgaa cttcgttgga atacgcttct gcttcacgaa
     6301 tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt
     6361 ccggcggacg agcagcctgg aagttgacgt ccagcagcgt gatacccatg tcatacggac
     6421 gaatcgtctc ttccagttcg cgctgagtat cgctacgaat cacggtacga ccttccgtca
     6481 gaatgcggtc catggtgtat ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac
     6541 gcaggctgtc atccgggctg gtcacgctat acagatattt ttccggattg gtgacgcggt
     6601 actgcacgtt catctcaacg cgcactacgt tctcgtccga cgtcagcatc acaccagaag
     6661 cggccagttc acgcacggct tccacgttca ccggtttgac ttcgtcgata aacgtcggtt
     6721 tccagttcag acccggctca accagatggc tgaatttacc aaagcgtgtt accacgccgc
     6781 gttcggcttc tttaatggta tagaaaccac tggccgccca gataatgaca atcgctgccg
     6841 ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg gccttgcgat gaactgccac
     6901 cgccagatcc ggtgccttta ccgccgccca gaccaccgag ctttttgctc agtttgcgga
     6961 agatatcatc taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca tttccctcag
     7021 agttgccgcc aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct
     7081 gattccacgc catgtttgtg ctccatattt gttatgcggt gatccctgtt gtctttcgac
     7141 gccagaggat atgactccac gcttcagacg ctacgccgtt agatcaggta atcgatcaac
     7201 gccggttctt gtttacagag gcgacgccag tcaacgatcg gcatacgaac ttgcagactt
     7261 acgctgccgt cctcctccat ccactctttt tctattgcct gaagctgata aaaacgactc
     7321 ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct gcgccacctc gccggaaagc
     7381 cgctccgtca aagcctgaaa aagctgtggt atccccgctc cggtctgtgc ggaaagccag
     7441 acacggatcg gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata
     7501 tcgatcttgt tcatcaccag cagggttggg atctcgtgag cgtcgatctc ttcaagaacc
     7561 gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc
     7621 agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa atgccgccac cagatcgtgc
     7681 ggcaggtggc gaataaaccc tacggtatct gcaagtacgg tttcaccgac atctgcaacg
     7741 tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc
     7801 gcttcggtga tgcgattgaa aagggtagat ttaccggcgt tggtatatcc caccagcgaa
     7861 acagtaggaa cgtcggcttt gatacgcgat tgccgcccct gctcacgctg cttttcaact
     7921 ctttccaggc gcgactgtat ctgcacgatg cgattacgca acaaacgacg gtcggtttcg
     7981 agctgggttt cacccggacc acgcaaacct atcccgcctt tctgtctttc aaggtgggtc
     8041 cagccacgca ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac ctgcaactta
     8101 ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt gcggtcgata
     8161 acacgacact cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag ggcatggtca
     8221 aaaagaacga ccgaagcacc catcgctttg acagcttccg caatttcaac tgctttacct
     8281 tcacctacaa aatactttgg gtgcggcgct ttacggctac cggtaatcac ctgcaatgct
     8341 tcgacaccgg cggaagagac cagagattca aactcctgga ggtcttccat atctttgtct
     8401 tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag catcataacg gtcaaacaag
     8461 cgtataaccc tctaaataga tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa
     8521 aacagcccga aaccttattc ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg
     8581 ctgctaccat gatggtagtt actgctggta ccgccaccgg cgttgttact gtgatgagaa
     8641 accgggcgag atgggacaac agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg
     8701 tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt gcccttgcag cttaatacca
     8761 ttcaccaaat aaatagaaac tggaacacgt tcccgacgca gtgcgttcag gaacggatct
     8821 tgtaaagatt gccccttagc cattctctct tttccttata tgcttatttg tactttgaac
     8881 ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt gtacacattc agcctgcgat
     8941 agcaccaaca acctgtaata cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca
     9001 gtgaacccct tcccaaccac gcagccaggt tatctgccgc ttcgccaact gtctcgtggc
     9061 gcaaacacct cgataaacca tttcatcgta tgagatttcg ccttcaaggt aagaccacat
     9121 ctggcgataa cccacgcaac gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa
     9181 aagcgcccgg acttctgctt caaaacctga agccaacatc tgatgaaaac gctgctcaat
     9241 gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg aactgatgca cctgatacgg
     9301 tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac
     9361 ttccagtgcc cgggaaagcc tttgtggatc atttggatga atccttgctg ccgcaaccgg
     9421 atctacctcc tgaagttgac gatgcaatga ctcccaacct tgctctgccg cctgttgctc
     9481 aattctggcc cgcacttccg ggtctgccga cggtagcggc gacaaccctt ccagcaatgc
     9541 cttgaaatac aacatcgtac cgcccactaa cagtgggatc cgccccgccg cggtgatatc
     9601 ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc
     9661 gcgaatatcc agcaatcggt gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt
     9721 cccgatatcc atccctttgt aaataaggac agaatcaacg cttatcaact ctactggtaa
     9781 aattttacgc agctcaatgg ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa
     9841 aatcgcctta ggcaggctcg ccttactgat atcactcatc tttcagggct tttatcgccg
     9901 gatgtaaatc aacagattgt aacagaccac ccggcggcgt tttcacaagt tgcggacata
     9961 accgttccac gtccgccagc agggttatgg cctgtgccat tgaccactgc gcatgttcgc
    10021 tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg ttcgaatacg gactgcttcg
    10081 ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag
    10141 gcacggccct gatggtcaca tgctgtgcat ctgactggaa atcaataccc aattccgcta
    10201 gggcagactg cgctttttct aatgccgatt tttcttcgcc agaaactttt agccgcaacg
    10261 gaatcagcag cggctgggcg caaacgggcg cttcacccgg cgtcaattgt gcctgacgca
    10321 gccaacgttc tgccactggc aaggataaaa gtgaaatgtt gccatcgcgc tccagcaacg
    10381 cacagtcgga atggacgata gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg
    10441 caggttcctg cggttccggc gcttttggtt tttgcatcgg cgcgggcgtt tgcaaaagct
    10501 ggcgatacac ttcaccttgc tgtttctggt agcctggctg cgcattcggc cagggggcag
    10561 ccggacgact gcctgaggcg ggcgcaggag agtagcgcgg agctaccggc tcacgagctg
    10621 ccggttctgc aaagtgattg cgccccgccg ccacgcggtt ttccgggatg gcacgcggtg
    10681 cgggttgtgg ttcatcgtcc agcggtagcg gcgtttccag ttgctgttgt agcacgctca
    10741 gcacgccctg atagataaaa tcatgcacca gacgcgactg atggaaacgc acttcgtgtt
    10801 tggcggggtg tacgttgacg tccacctgat gcgggtcgat ctccagatac aacacaaatg
    10861 ccggttgctg atcggccccc agtttgtctt cgcaggcctg gcggatcgca tgattgatca
    10921 ggcgatcgcg catcatgcga ccattcacgt agcaatactg aatttccgcc agtgcgggcg
    10981 tagtgtgatt tggatcggcc acccagccgc gcagggtgag atcgccgtgt tgccattcaa
    11041 tcgccagcgc ttgttcgaga aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt
    11101 gcccgccttc cggcactgcg cggtactgac gcacaatttt accgttatgc gacaggttga
    11161 tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat ttcatcaata tggctaaatt
    11221 cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt gttgtagaac agatccagca
    11281 cctccagcgt cgttcccacc ggatgcgctg ccggtttaac cgtcacgtcc atgtcgcgtc
    11341 cttcggcata ggcctgccag gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc
    11401 gggaaaccga actgatactc gccagcgcct caccgcgaaa gcccaggctg ataatggctt
    11461 cgagatcgtc cagagaggcg attttactgg tggcatgacg agccagcgcc agcgccagct
    11521 catctttttt gataccgcag ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt
    11581 cgatatcaat atcgatacgc gtcgcacctg catcgaggct gttttccact agttctttga
    11641 ctaccgacgc aggtcgctcg accacctcac ctgcggcaat ctggttcgcc agttgtggcg
    11701 gtaagacctg aattggcatc aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca
    11761 ccgtactggc agtttgtgcc gttgcaccct gcggcgcaga ttgcatcgga tgcgcaagga
    11821 agtaattgcg caggcctttg taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg
    11881 ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt ttcgaccagt actgatggga
    11941 tatccggcga gcgcagaacg ccaaggctgg cgtgttctgg tcgacgttta tgtatttcgc
    12001 caatgcgttg caactgactg atcatactgg tcgctacatc ataccctacc cgctgggaat
    12061 gaccgaactg taaatccagc accgcctggc ttaaataggg gtcagactga ctgttcgcca
    12121 gcacatcacc cgccccaccc agcagctccg acagtttctc gtgctgctcc agccagctgg
    12181 ccatttcact gttggcgcga cggttagaga gcacccatac ggaagcgcca gtcgcactac
    12241 ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt ggcgttttgc ttatgtgcca
    12301 catcactgcg ccccatcacc gagataaagt aatccccgtc acgggttaaa acgcctttaa
    12361 acatcggatc gtcattgagc aaagtacgca atttacgcgc gatggcgatg gtgacatttt
    12421 tctcccgcgt accaccgggg ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa
    12481 tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg
    12541 tattactgct gataacaccc gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg
    12601 gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac gcgtttcgca accacgggcg
    12661 gcggaggagg cggtggcggc acatcggcgt taatcgtaaa gacgacagtg taattgctgc
    12721 cattctgccg cttcaccgct tcggttttac cgttttcggt aagatcgacc accagccgta
    12781 gcgtttgtgc atctttaggc gttccagagc gaatcgcctt caccagatta ttgccgctga
    12841 acaacaacgg cagtccctga atcatgcccg tttgtttgat atcgagcgcc acggtgcgtt
    12901 tgctttgatg gctaaacgca taatcaggat cgccaataaa actcaacgtt atccgcgcct
    12961 gttgattacc gttagaaacc tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca
    13021 gcagcagcag cgtcgctacc aaccaatttc tgatgcgata catcatcccg ccacctttca
    13081 aaggttaacc ggctaaacgc gccagcaaca attcacccgc agaggaaacc gcactcacgc
    13141 gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc gacatccggg tcaggaagaa
    13201 cacctgtacc ttgttgtggc cactccacca ggcagatggc atcgttggca aaataatcgc
    13261 ggatccccat aaactccagc tcctcgggat cggcaaggcg gtacaaatca aagtgataga
    13321 ccattaagtt gtcgagcgta tagggttcga ccagcgtata agtggggctt ttgacattac
    13381 cctgatgacc cagagcctgt aaaaagcccc ggctaaaggt ggttttacct gcgcctaaat
    13441 cgccatacag atagattacg gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt
    13501 ctaatgttgc ctgctcatca gggagcggaa ttactcgatt catcatggtt tttatcagtc
    13561 acttccgggt taacaatacg ctgtagcgtg gaaaagagat cggttgccag catcccgcgc
    13621 gttccaaaac gcgccgccag tacgtcagct gccgcaccgt gcgcgacaca gcctgcacag
    13681 gctgcatcat acggcgacag tttttgccca agcaatgcgc caataatacc agagagcaca
    13741 tcgcccatgc cgccgctcgc catgcctgca tttccggcat caataatgcc taaagcgtca
    13801 ggatgggcgg cgaccacggt tccggcacct ttcagcaccg ctacgccgcc ataacgttgt
    13861 accagacgtt tggcgcaatg taagcggtca ctttcaattt cagcgacgga acagcctaac
    13921 aaccgtgcgg cctcgctagg atgcggcgtg atcacgcgat tgtgacgctt atcgggattg
    13981 attgccagca ggttcaatgc atcggcatcc cacaacatcg gtttgcgaaa attctcaact
    14041 ttttgcaggg cttttttccc ccactcttgc tggcccagac cgggaccaat caccaccaca
    14101 tcggcccatt ccaggctttc ggtaagagag tccatagtca gttcatgcac catcaattct
    14161 ggtcgtgcag tcagcagcgg cgcaatgttc tcactgcggg tcagtactcg gactaaacca
    14221 gcaccagcac gtagcgccgc ttcccccgtc atacgaatag cccccgccgt gccgtgatcg
    14281 ccaccgataa tcaccagccg cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc
    14341 agccagtgag aaagttgttc tgccgaaaac cgctgaattt tcgtctcctg acctgccagc
    14401 cagctatcca gccccagtga gtcaaaatgc agttgtcctg taacatcccg cgcttttcca
    14461 gtgagcaagc ctagtttcag cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg
    14521 cctggcgtag cgccggtttc agccagcagg ccggaaggga tatcaaccgc cacaatcggc
    14581 gcaggatggg aattagcgtg gtcgattaac tggctaatgg attcgcgggg cgcttgctgc
    14641 aagccggtac cgagcagcgc atcaacaatc agatctaccg attcgggcca gacaatattc
    14701 gaagcatgga tctcgccacc cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc
    14761 ggcaacggtt tgtcgctctc ctgggccaac aacgtgacct caatgccgac cgctttggcc
    14821 agtcgcgcga ccacgtagcc atcgccgccg ttattaccat gaccgcacag caccagccag
    14881 tggcgggcgt caggatacgc cgaacgacac acctggaatg cggcctcgcc agcgcgaagc
    14941 atcagctcat agagtgtgag ccccagcaca tctgccgcct cgcgttctcc gcggcggata
    15001 tcgtcggcgt accagacggt gtgtggtata cttacggggt ttttcttcat tgtatggtcc
    15061 gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg
    15121 gaactgggct ttcagcaggt aggtattacc gataccgatc tcagcgagtc cgagcccaaa
    15181 ctgcaagcat ggctggacaa acaataccac ggcgaaatgg actggatggc acgtcacggt
    15241 atgctgcgcg ctcgccccca tgagttatta cccggtacgc tgcgtgtaat cagcgtgcgg
    15301 atgaattacc ttcctgctaa cgccgcattt gccagcacgc tgaaaaaccc caaactcggc
    15361 tatgttagcc gttatgcgct gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa
    15421 aagctgggcg agatgattca gcaacattgt gtttcgctga attttagacc gtttgtcgat
    15481 tctgcgccta ttctcgagcg cccgttagct gaaaaagctg ggctcggctg gacaggtaag
    15541 cactcactta tcctcaatcg cgaggccggt tcgttcttct ttttaggcga attgctggtc
    15601 gatattccgc tgcccgtgga tcaaccagtc gaggaaggat gcggcaaatg cgtggcctgt
    15661 atgacgattt gcccgaccgg tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt
    15721 atctcttatc tcaccatcga acttgaaggg gcgctcccgg aagagttgcg accgttaatg
    15781 ggaaaccgta tttacggttg cgatgactgc cagcttatct gcccgtggaa tcgctattcg
    15841 caactcacta cagaagacga tttcagcccg cgtaagccgc tacacgcacc ggaactcatt
    15901 gagttatttg cctggagcga agagaagttt ttaaaagtca cggaaggatc ggcgattcgc
    15961 cgtataggtc acctgcgctg gctgcgtaat atcgccgtgg cattaggcaa tgccccttgg
    16021 gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg agcacccact tctcgatgag
    16081 cacatagcgt gggcgattgc gcagcaaata gagagacgaa atgcgtgcat agtcgaagtg
    16141 caattgccga aaaaacagcg tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc
    16201 tgattcatcc acagcttgtg aataaaaata aaaacgcatt gcaattcaag acgaataaaa
    16261 tcgtcaagtg atcgactcga caaaacggaa tcttgatttt taacttattt atcaataggt
    16321 taaaaaaata ccattcaccg tgcgaagttt ctttgaaatt aagattacag cctgaggctg
    16381 tggataactc tgtttacaag agtttgtcag agacaacaaa aaccagcaat aacgcatcgc
    16441 gtcgctgtag atattttatt gagagaaga
//
LOCUS       DAJTYL010000090        16454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYL010000090 DAJTYL010000000
VERSION     DAJTYL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.090"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            98..664
                     /gene="mntP"
                     /locus_tag="OO572_002849"
     CDS             98..664
                     /gene="mntP"
                     /locus_tag="OO572_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6661784.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(661..1470)
                     /gene="rlmA"
                     /locus_tag="OO572_002850"
     CDS             complement(661..1470)
                     /gene="rlmA"
                     /locus_tag="OO572_002850"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6661785.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1636..1845)
                     /gene="cspE"
                     /locus_tag="OO572_002851"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1636..1845)
                     /gene="cspE"
                     /locus_tag="OO572_002851"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6661786.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(1858..2001)
                     /locus_tag="OO572_002852"
     CDS             complement(1858..2001)
                     /locus_tag="OO572_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6661787.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2672..2959)
                     /locus_tag="OO572_002853"
     CDS             complement(2672..2959)
                     /locus_tag="OO572_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6661788.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3034..3177)
                     /gene="mgrB"
                     /locus_tag="OO572_002854"
     CDS             complement(3034..3177)
                     /gene="mgrB"
                     /locus_tag="OO572_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6661789.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3336..3575
                     /locus_tag="OO572_002855"
     CDS             3336..3575
                     /locus_tag="OO572_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661790.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3718..4509)
                     /gene="kdgR"
                     /locus_tag="OO572_002856"
     CDS             complement(3718..4509)
                     /gene="kdgR"
                     /locus_tag="OO572_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6661791.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4686..6059
                     /locus_tag="OO572_002857"
     CDS             4686..6059
                     /locus_tag="OO572_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661792.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6105..6986)
                     /gene="htpX"
                     /locus_tag="OO572_002858"
     CDS             complement(6105..6986)
                     /gene="htpX"
                     /locus_tag="OO572_002858"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6661793.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7178..9226)
                     /gene="prc"
                     /locus_tag="OO572_002859"
     CDS             complement(7178..9226)
                     /gene="prc"
                     /locus_tag="OO572_002859"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6661794.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9246..9944)
                     /gene="proQ"
                     /locus_tag="OO572_002860"
     CDS             complement(9246..9944)
                     /gene="proQ"
                     /locus_tag="OO572_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6661795.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10041..10538)
                     /gene="msrC"
                     /locus_tag="OO572_002861"
     CDS             complement(10041..10538)
                     /gene="msrC"
                     /locus_tag="OO572_002861"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6661796.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10668..11951
                     /gene="yebS"
                     /locus_tag="OO572_002862"
     CDS             10668..11951
                     /gene="yebS"
                     /locus_tag="OO572_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6661797.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            11920..14540
                     /gene="yebT"
                     /locus_tag="OO572_002863"
                     /pseudo
     CDS             11920..14540
                     /gene="yebT"
                     /locus_tag="OO572_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14620..16059
                     /gene="rsmF"
                     /locus_tag="OO572_002864"
                     /pseudo
     CDS             14620..16059
                     /gene="rsmF"
                     /locus_tag="OO572_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16177..16413
                     /locus_tag="OO572_002865"
     CDS             16177..16413
                     /locus_tag="OO572_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6661798.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
BASE COUNT         3959 a         4183 c         4000 g         4312 t
ORIGIN      
        1 gcatgaacgc gaaaacccat cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga
       61 tgatgtgttt tatggatacc ccggtcagga cgttgtcatg aatatcactg ctactgttct
      121 tcttgcgttt ggtatgtcga tggatgcatt tgctgcatca atcggtaaag gtgcccccct
      181 ccataaaccg aaattttctg aagcattgcg aaccggcctt atttttggtg ccgtcgaaac
      241 cctgacgccg ctgatcggct ggggaatggg catgttagcc agccggtttg tccttgaatg
      301 gaatcactgg attgcgtttg tgctgctgat attcctcggc gggcgaatga ttattgaggg
      361 ttttcgtggc gcagatgatg aagatgaaga gccgcgccgt cggcacggtt tctggctact
      421 ggtaaccacc gcgattgcca ccagcctgga tgccatggct gtgggtgttg gtcttgcttt
      481 ccttcaggtc aacattatcg cgaccgcatt ggccattggt tgtgcaacct tgattatgtc
      541 gacattaggg atgatggttg gtcgctttat cggctcaatt attgggaaaa aagcggaaat
      601 tctcggcggg ctggtgctga tcggcatcgg cgtccagatc ctctggacgc acttccacgg
      661 ttaataagaa cgctgccaga ggtgaatatt aaagtccgtc tggcagtcga acacttcttt
      721 tgctgccagt gtttgccaga cttctggctt cgcacgccag gcaaacggcg tcatctgcaa
      781 taatgcgacg gcttcatcac cgcgaagacg catcggataa cacaactccg cactctgctg
      841 taatgtaaaa ccttccagtt gttctgcatg aggtgcatga agatgtactt cattgtaaat
      901 cagccccttc agctccatca aatgtcgcgg tcccggcgtg gcagtaatga cccagccgcc
      961 gggcttcact actcgtgcta attcttctgc tttacacggc gcgtaaatac gtattatggc
     1021 gtccatactg gtatcggaaa acggcaaacg gtggctggaa gcgacacaaa gagtgacctg
     1081 cggatagcgt ttcgccgccg cttttatcgc taccttcgag acatccaggc caaacgtggt
     1141 gatttcgggc aacgcatcgg caaatgcgtg tgtgtaatac ccttcaccac agccaatatc
     1201 cagcaccgcc gtggcctttt catcaagccg ttcccgcagt tgggcgacaa ttgcatcacg
     1261 cagcggctga taatgtccgg catctaagaa tgcgcggcgt gcttgcatca tttccgcgct
     1321 gtcgcccgga tcacgagacc gtttatgctg aacgggcagc agattgacat acccttcttt
     1381 cgccatatca aactgatgtc gttggggaca gatatagctg tttttttcac gcgaaagagg
     1441 ctgatggcaa agtggacagg aaaacgacat gacaactccg gcaggatact aaaggccgca
     1501 agtgtaacgc gaatcacgcg gcgggcaaat atccgaacgc caggagcgaa gataaagcat
     1561 agtaaaaagc ctcgcattcg acgaggcttt atagcgagat tgaagcgtat tcacacttca
     1621 gatcagtgga ttcgatcaga tagctgttac gttaacagct gccggacctt tctggccgtc
     1681 ctgaatttcg aactcaacgt tctgaccttc agccagagtt ttgaagccat taccctggat
     1741 agcggagaag tgtacgaaca catctttgct gccatcagcc ggagtaatga agccaaaacc
     1801 tttagactcg ttgaaccact taacctgacc tttaatcttt gccatttgaa aaattcctta
     1861 gattgttttc ttcgcccgca ggcataacat agataaaact gacacattac tgcatgaggc
     1921 accaatataa ggctcggcag agaagcggta ttcaacgtca acgtgtttac tcaggacttc
     1981 tttactgaaa atgccacaca taaacagaac tgtacctcgt ttaacccgaa atcttttatc
     2041 acatacaacg ttaattatgg caagccattt ttaaacatat ctcgatcaga cgcacaaatc
     2101 tcgatacgtc ttccactttt tttgcacact tatgcaacgg aatacgcgcc gatatatcat
     2161 tgtgcttaac cttgccagtt caggcagata cttaaactgg cgtagtttct aacatagttc
     2221 aataagttca gttgttccag gccagaggga gaagttatta catagtgcgt gcaatatcac
     2281 attttctgct atgcaatgaa taaaaagtta tatcactttt tctcataaaa cagtcagtta
     2341 acggctatca attaccctaa agagaagtca atccccaaag ggattgtaaa tttaaaataa
     2401 gaaaaattga tgagtgagca aaaaaaatca agagagaaac gtttcgctaa taaataatac
     2461 cacgcttaat cacaaatctg atgtaaacat cctaataaat taatgaggaa aatatctttt
     2521 tctgatatcc cgttacgcaa ataaataata tgataattat ttgaagtcag accagagttt
     2581 taatttgaag cagttgcacc atgacagtgc gtataatcag gatgaattga gtaacccttc
     2641 aacgacgggg ttactcaaga ttgaggaagg attatcgttc ggcgaccagg cgaataatat
     2701 cttcgtcatc aaccgatttt tgactctcaa tggtcttttt atctgctttc tctggttcgt
     2761 ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc agcaacgcct
     2821 caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa aaccacaaga
     2881 tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
     2941 aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg
     3001 agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
     3061 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
     3121 aagcagcaag caagccaaca ccacgacaac cagaacgacc catcgaaact ttttcactcc
     3181 aatctccgct tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
     3241 actatgtcga tattcgtgct ttttcggaat gagtagcttg cttcagcgca tattgccgct
     3301 acgattaagc gaacataaaa agagaagaga ttgtaatgcg attcatcatt cgcacagtta
     3361 tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
     3421 gcaaagagaa tgccgcagga ttaggtttgc aatgtaccta tctgactgcc agaggcacca
     3481 gcacggtaca atacttgcac actaaaagtg gtttcttgga gataacagat tgcccactgt
     3541 tacgtaaaag caatatcgtg gtcgataatg gttaagggta ataaataacc agattctcac
     3601 tcatgagtaa aatattctta tagcaagcgc gtttaatata acgcaccttc aactttattt
     3661 ttctcatgct aaataaagaa cctgtctgtt tgatattccg acaggttctt actctcatca
     3721 gaacggatag tcgtgataac ccatttgggc agaaattttg cgcgctgcgg tatgcaacat
     3781 tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg agatgctcaa
     3841 accggcaatg accacgccaa agcgatcaaa taccggtacc gcaatgcatt gcagcccttc
     3901 ttcctgctct tcgttatctt ctccataccc ctgctcgcgc acctggtcca gaacgggtaa
     3961 taacgcttct gtactggtga tggtccgctc ggtactgcgt ttatactcca cgccctcaag
     4021 aatttgcttc acttcatcgc gatcgcgcca tgccagcagt accttaccaa tcgcggtgct
     4081 gtacagcgga ttacgacgcc caatccgtga atacatgcgc aaattgtaca tagagtcaat
     4141 tttgtgaatg taaacaatac tgtcttcgtc cagtgcgccg aggtggatag tttctttggt
     4201 caggcgggag atctcacgca tctggatatc tgcgctacga attaaatcga cgttttgtaa
     4261 cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa tatttctccg actccccttc
     4321 ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag cgataaacgg tgctttttga
     4381 catcatgacg cgctgcgaca gctcggttat ccctatttcg cgctcttcac ccagcgcctg
     4441 caaaatgcca aaaactttta gcacggaaga tacagaatca ggctgtttat ccagatctgc
     4501 gttagccatt tatcacctca ttgcgagtgt tttataaaaa tcagaactgt tttttattat
     4561 aatttcgcac cagggtggtc gcaatccatc ttttgccggt tagttacaat tctgcgacat
     4621 ccaccgtgaa tatcagtgct agaatcatac ccctgttgat tattcaccaa agatataaaa
     4681 ttcctatgcc aaaagttcag gccgacggcc tgccattgcc ccagcgatac ggtgcgatat
     4741 taaccattgt gattggtatt tcgatggctg tccttgacgg cgcaatcgcc aacgtcgccc
     4801 tgccaacaat tgccacggac cttcatgcca caccagccag ttccatctgg gtagtgaacg
     4861 cctatcaaat cgccattgtc atctccctgc tctcgttttc gtttctgggc gatatgtttg
     4921 gctatcgacg tatttataaa tgcggtctgg tcgtttttct gttgtcttca ctgttctgcg
     4981 ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt ttcggcggtg
     5041 cagcgttgat gagcgttaat accgcactta tccgcctgat ctatccacaa cgttttctgg
     5101 ggagagggat gggcataaac tcgtttattg ttgccgtctc ttctgctgcc gggccgacaa
     5161 ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt atttttaatc aacgtaccgt
     5221 taggtattat cgccctgctt ctggcgatgc gttttctgcc acccaatggt tctcgcgcca
     5281 gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc gttaaccttc ggcctgctta
     5341 tcactgcgtt gagtggtttc gctcaggggc aatcgctgac gttaattgct gcggaactgg
     5401 tggtaatggt tgttgttggt attttcttta ttcgccgcca gctttctctt cccgtaccgc
     5461 tgctaccggt ggatttactg cgtatcccgc tgttttcact ttctatttgc acatctgttt
     5521 gctctttctg cgcacaaatg ctggcaatgg tttccctgcc cttttacctg caaaccgtgc
     5581 tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc gtggccgtta gcaacgatgg
     5641 tgatggctcc tctggcaggc tatttgattg aacgcgtaca tgcaggattg ctgggtgctt
     5701 tagggttgtt catcatggct acggggcttt tttccctggt tctgctgccc gcgtcacctg
     5761 cggatatcaa tattatctgg ccgatgatct tatgtggcgc tggatttggc ttattccagt
     5821 cacccaataa ccacaccatt attacctccg ctccgcgcga acgtagcggt ggggccagtg
     5881 gcatgttagg aacggctcgt ctactgggtc agagtagtgg cgcggcgctg gtggcgctga
     5941 tgctaaatca gtttggtgat aatggtacgc acgtctcgct gatggctgcg gctattctgg
     6001 cagtgattgc tgcctgtgtc agtggtttac gtatcactca gccacgagcc agggcataaa
     6061 aaaagcgcgt cgatcaggac gcgcttttta gtatttactt catattactt caggtattca
     6121 cccgtacgca gagcttcaat acgtttatcc agcggcgggt gagtcatgaa caactcactg
     6181 agcgatttcg acttaccgtt aatgcagaaa gccatcatgc tggttgcttc ttgcggttca
     6241 tagctggttt tcaggcgctg cagcgcggca atcattttct cgcgaccaac cagttttgcc
     6301 gaaccggcat ctgcatggaa ttcacgatga cgcgagaacc acatggtgat aatgctcgcc
     6361 agaataccaa acaccagttc cagaaccgtt gcaaccgcaa agtagatcag cgggttgccg
     6421 ttgctctctt caccttcatc acgatttccg cccataaaac ccgcggcaag ctgcgccaga
     6481 atacgggaaa taaagataac gaaggtgttc accacgccct gaatcagcgt catggtgacc
     6541 atatcaccat tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc ttcatccggg
     6601 ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg gcgcgcaccg
     6661 gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca cttgcggcat agcgatcccc
     6721 gcctgacgag cctgggttgc tacagtattg accagccaac gttccctttc gttacgcggt
     6781 tgctcgatca cttccccgcc aacagatcgt aatgccatcc atttggacat cagaagcgaa
     6841 acgaaggaac caccaaaacc gaacagcaag gccatgatca tcagcccctg aacgctgctc
     6901 gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga ccattacggc caggttcgtt
     6961 agcaggaaga gcgcgattcg catcataatt ttctttttac ctcagtttaa caaaacgcaa
     7021 tatgcgatac ccacatcgta tgggttacgc gactattttc aagtctggat agtgcgtaag
     7081 tcaccagaaa gacacaactt tacattttgt agcatctgat ttacggcatc ttgccgctgt
     7141 taaaaaatca ggcacaattt cttgtgcctg attgatatta cttgacggga gcgggttgtt
     7201 ccgcgggtct ggctttttca agcttcgcca gatcgagtgc gatattcacc gtctcatcca
     7261 gataaggatc cggctcctgg taatctttcg atagatcatc cagcttcttc aactccggtt
     7321 taccttctcg tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca tcttcattat
     7381 tctctttctc acgcacagcg tagttcagag aaacgatatt gcgcttgtcc ttcatagcgt
     7441 tgaagcgcgc gatatccttc atgatgttct ggaactcagg atctttcgcg atacgcgcat
     7501 tatgttcctt cagcagctcc ggttcaaagg cagttaaatc tcctgatttc acataagtcg
     7561 cggcatcgat gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc gtttccgttt
     7621 cttcattacc cgtcggcatg atgatgtctg gtgttacgcc tttacgttgc gtactgccgc
     7681 cgttaacgcg atagaatttc tggatcgtgt actgcacaga acccagcgct ggccattcag
     7741 gacgtaacat ctgatcgtaa atacggttca atgaacggta ttgctgaacg gtgcctttac
     7801 caaacgtcgg ttcacccaca accagcgcac gaccgtaatc ctgcattgcc gcggcaaaga
     7861 tttctgaagc cgaagcactg aaacggtcaa ccagtaccac cagcgggcct ttatagaaca
     7921 cctgcccgtc ggtatcgctg tcttcacgaa ccttgccgtt gttatcgcgg acctgaacaa
     7981 tgggacccgc aggaataaac agacctgaga gcgataccgc ttcggtcaac gccccaccgc
     8041 cattgctacg caggtcgatg atcacactgc tgacattctg tttttccagt ttctgcagtt
     8101 gaactttgac atcgtctgtc aaacccacat agaagcccgg aatatccagc acgccgactt
     8161 tctctttacc gacggtcttc accgacattt taaccgcgcg gtcttcgaga cgaatacgtt
     8221 cacgggtcaa cgttacagta cgggtcttgg tccctttacc agcaggtaaa atttccagac
     8281 gaactttact gcccttcggc cctttaatta aggcaaccac atcatcaaga cgccagccaa
     8341 tcacgtcaac catcggcttg cctgtttgac caacaccgac aattttgtca ccaacgctga
     8401 tagctttact cttcgctgcc ggaccacctg ccaccatcga attgataacg gtgtagtcat
     8461 catccatttg cagcactgcg ccaatacctt ccagtgacaa actcatttca gtgttgaact
     8521 gttcggtatt acgcggggaa agatagttgg tatgcggatc gatttcacgc gcaaacgccg
     8581 tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga atggcaaatt
     8641 tgtagcggcg tgtcagggtt tcacgaattt ctttatccgt ttttcctgtc agcttcaggc
     8701 ttaactcgtc gaatttgact ttactgtccc actgcgcgtt caactcagcc tcgtttttcg
     8761 gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa tccatcggct
     8821 tttccagtac cgacaaagcg tactggtaac gttcaaaacg gcgcttttgc gccagattgt
     8881 agagatcgta gaaaacgtcg agtttgcctg aacgcagttc atcgcctaac tcggttttct
     8941 ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacatt gtggctgtaa tcgagcagat
     9001 tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac tggcgataat
     9061 gagaacgggt gaagcgcgac gttacgcgct cacttaccgt cgcatgctgc gtctcttcct
     9121 ttaataccgg aatttgatca gcacgcgtga tatcttctac agcgaaggtc tggcctgcta
     9181 ttgcaagcag gccagctaac gcggtaagcc taaaaaacat gttcatgcct ggcccggcct
     9241 ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag acatacccga attcagctgg
     9301 acgcggacgc cgtctttggt gatttctaat acggtggcat ccatcgcgtt ttgacccgct
     9361 ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt cagaaaccgg ggtgtgctgt
     9421 tcttcgcgag gtgcttttac tgtttttggc gctttctcta ccggcttttg cgcacgaggt
     9481 ttacgttcag cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg ttcgcggcgc
     9541 ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc gttttttcgc ttgctgttca
     9601 gcacgctgtg cctgaacacg cgctttcgct tcttcaagct gcttgcgagc atgctctaca
     9661 tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat cgacacgcgt tgcgccgggt
     9721 ttaacaccgt aaagataacg ccagctcgaa gtgtagagac gtaaagcgga tcgtaattgc
     9781 gttttgctca ggttcatttc cccggcaaca cgatcgacca aatcctgaaa aataccaatt
     9841 ttcagcggac gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc ggccagaaac
     9901 gcgattactt ctttactgct attcaactta ggttgatttt ccatgaaatt tcctgattac
     9961 aacggacgta gccaacaagc gcaggcatga acaggcgtca ttataatgac gctaccagta
    10021 aatgctacgt tatccgttga ttatcctgcg acgctcgcaa agaatttttt gtaatccgtc
    10081 gttgcaagca ctttttcaag ctgtgccaca agctgacgta agccttgctc gtcctcgtct
    10141 gtaaagcgag cgaagacagt actatcgatg tcgagaacac caataatctg atttttcacc
    10201 accagcggca gaacaatttc agaattactc gccgcatcac aggcaatatg cccgtcaaac
    10261 gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa ccgctgtgcc gcacaccccg
    10321 cgcccgacgg gtatccggac acaggcaatt ttgccctgaa atggtccgag taccagtgta
    10381 tcgtcctcaa gcaaataaaa acctgcccag ttaacgtcag tgagacgctc gtataacaac
    10441 gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc ccgccatcag cgcgttaaag
    10501 tcgcgattta aatccgcgta aaattctgtt ttgttcatta cttaatcact tggttgtctt
    10561 acctggaact gccagcctat taaaataagc attaaatgcg ttaatgctca agatcattcc
    10621 catcatgggt taagattaat gttaattctt attacatttg gcacgtcatg gctcttaaca
    10681 caccacaaat tacgccgaca aaaaagataa cagtgagggc aatcggcgag gaactgccgc
    10741 gtggtgatta ccaacgttgc ccgcaatgtg acatgctgtt tagcctgccc gagataaatt
    10801 ctcatcaaag tgcctattgt ccgcgctgtc aggcaaaaat tcgcgacggg cgcgactggt
    10861 cgctaacgcg cctggcggca atggccttca ccatgctgtt gcttatgccg tttgcctggg
    10921 gcgaaccgct gttgcatatc tggctgttag gcattcgtat tgacgccaac gtcatgcaag
    10981 gcatctggca aatgaccaaa cagggcgatg cgataacggg atcaatggtc tttttctgcg
    11041 ttattggtgc ccccctcatt ctggtgtcct ccatagcata tttatggttt ggtaaccgac
    11101 tgggaatgaa tttacgtccg gtactgctga tgcttgagcg acttaaagag tgggtaatgc
    11161 tggatatcta cctggtcggc attggcgttg cttctataaa ggtacaggat tatgcccata
    11221 tccaggcggg tgtcggcttg ttctcttttg tggcgttggt gattttaacg acggtgacgt
    11281 tgtcacatct taatgttgag gagctgtggg agcgatatta tccgcagcgc cccgctacgc
    11341 gtagggacga gaaacttcgt gtctgtcttg ggtgccattt taccggctat cctgatcagc
    11401 gtggtctctg cccgcgttgc catatcccgc tacgcctgcg tcgtcgtcat agtttgcaaa
    11461 aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt accagccaac ctattgccta
    11521 tttctatcat ttatctgaat ggtggccggc aggaagatac gattctttcc ggaattatgt
    11581 cgctggcaag tagcaacatt gcggttgcgg gaatcgtgtt tatcgccagt attctggtac
    11641 cgtttactaa agtgatcgtc atgttcactt tactgttgag cattcatttt aaatgccagc
    11701 aaggtttacg cacacgcatt ctgttactgc ggatggtgac ctggattgga cgctggtcga
    11761 tactcgacct gtttgtcata tctttaacca tgtcgctgat taatcgcgat cagatcctcg
    11821 cttttactat gggaccggct gcgttttatt ttggcgctgc cgtaattttg actattcttg
    11881 ctgtggaatg gctggacagc cgcttacttt gggatgcaca tgagtcagga aacgcccgct
    11941 tcgacgactg aagcgcagat taaaaataaa cgccgtatct cacctttctg gctgctgcct
    12001 ttcatcgcgc taatgattgc cggttggctg atttgggaca gttatcagga ccggggtaat
    12061 accgtcacca tcgactttat gtcggcggat ggtattgttc cgggccgtac gcctgttcgt
    12121 tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc tcagcgacga tcttcgtaag
    12181 attgaagtca aggtcagcat caagtccgat atgaaagatg cgctgcgcga agagactcag
    12241 ttctggctga tgacgccaaa agcatcgttg gcaggtgtct ccgggctgga cgccctcgtc
    12301 ggtgggaact atatcggcat gatgccgggt aaaggtaaag agcaggatca ctttgtcgcg
    12361 ctcgataccc aaccgaaata tcggctggac aatggcgatc tgatgatcca cctgcaagcc
    12421 cccgctctcg gttcgctgaa cagcggttca ttggtctatt tccgcaagat cccggtggga
    12481 aaagtctacg actatgccat caatcccaac aagcaaggcg tggtgattga tgtcctgatc
    12541 gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt tctgaaacgt ttccggcgtt
    12601 gatgccaacg tcagtatcag tggcgcgaag gtgaaactgg aaagtctggc ggcactggtt
    12661 aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac ctgccgaggc ggaagatacc
    12721 tttggtctgt atgaagatct ggcccacagc cagcgtggag taataataaa actggaactg
    12781 ccgagtggag caggattaac cgccgactcg acgccgttaa tgtatcaggg gctggaagtc
    12841 ggacagctga ctaaactgga tttaaatcct ggtggtaaag tcactgggga aatgaccgtt
    12901 gatccctgct tcgtgaaaat acccgcatcg aattacgcaa cccgaaatta tcccttagcg
    12961 atgctaatct cagcgccctg ctgaccggaa aaacctttga gttggtgccc ggcgatggcg
    13021 agccacgcaa agagttcgtt gttgtgccgg gcgaaaaagc cctgctgcaa gagccggacg
    13081 ttctgacact gaccctgaca gcaccggaaa gttacggtat tgatgcgggt cagccgctca
    13141 ttcttcacgg cgtgcaggta ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca
    13201 cctttaccgt cgccatcgag cctcagcatc gagaactggt aaaaggcgat agcaaatttg
    13261 tcgtcaacag ccgtgtcgac gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca
    13321 gcgcctcaga atggattaac ggcgggatac gtattctgcc gggcgataaa ggcgagatga
    13381 aagccagcta tccactgtat gccaatctgg aaaaagcgct ggagaacagc cttagcgatt
    13441 tacccaccac aaccatgagt ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg
    13501 tagtgctgta ccgtaaattt gaagttggtg aagttattac cgtgcgtccg cgagctaacg
    13561 cgttcgatat cgatctgcac attaaaccgg aatatcgcaa ccttttgacc agcaacagcg
    13621 tgttctgggc agaaggcggg gcgaaagttc agctgaatgg tagtggcctg accgtacagg
    13681 catccccgct ctccagagca ttaaaggggg ccattagctt cgataacctc agtggtgcca
    13741 gcgccagtca gcgtaaaggc gacaagcgaa ttctgtatgc ttccgaaaca gcggcccgtg
    13801 cggttggcag gcagattacg cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc
    13861 caattcgcta tctcggtatt gatatcgggc aaatccagac gctggatctg attaccgcac
    13921 gcaatgaagt acaggcaaag gcggtgctct atccggaata tgtccagacc tttgctcgcg
    13981 gtggtacgcg cttctcagtg gtcacaccgc aaatctcggc agctggcgtt gagcatctag
    14041 atactatcct ccagccgtat atcaacgtcg aaccaggccg tggcaatcct cgtcgcgact
    14101 ttgaattaga agaggccacc attactgatt cgcgttacct agatggttta agcattattg
    14161 ttgaagcgcc ggaagccggt tcgttaggca tcggtacgcc tgtgctgttc cgtggtctgg
    14221 aagtaggtac ggttaccgga atgacgctgg ggacattgtc agatcgcgtg atgattgcga
    14281 tgcgcatcag taaacgctat caacacctgg tgcgtaacaa ttccgtcttc tggttggcat
    14341 cgggttacag tctggacttt ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc
    14401 aatttatccg tggcggcatc gccttcgcca cgcctccggg tacgccactt gcaccgaaag
    14461 cccaagaagg caaacacttc ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg
    14521 gaactgcgct tcccaaataa tgcccactgc tccggcgtgc ctgcgccgga gcgcttatgc
    14581 taaactgcgc gcctgttttt ttgccagtgg tacatgctcg tggcccaaca caccgtttat
    14641 ttcccggacg cgtttctgac acaaatgcgc gaagctatgc cttcgacgct ctcatttgat
    14701 gattttcttg ccgcctgtca gcgcccgttg cgccgcagca ttcgcgttaa tacgctgaaa
    14761 atctccgttg ctgatttcct gcaattaacc gctccttatg gctggacgct tacgccaatt
    14821 ccgtggtgtg aagaaggttt ctggattgaa cgcgacaatg aagatgcatt gccattgggt
    14881 agtaccaccg agcatttaag tggcctgttt tatattcagg aagccagttc aatgttgccc
    14941 gtcgccgcct tgtttgctga cggtaatgca ccacagcggg tgatggatgt cgctgctgcg
    15001 ccaggctcaa aaacgacgca aattgccgcg cggatgaata acgaaggggc aatccttgcc
    15061 aatgagtttt ccgccagttg ggtaaaagtg ttacatgcca atatcagccg ctgtggcatc
    15121 agtaatgttg cgctcacaca ttttggtggc cgcgtgtttg gtgcggcagt gccagaaatg
    15181 tttgatgcca ttttgctgga cgctccctgc tccggcgaag gcgtggtgcg taaagatccc
    15241 gatgcgctaa aaaactggtc acccgaaagc aatcaggaaa tcgcagctac ccaacgggaa
    15301 ctgattgaca gcgcctttca tgcattacgt cctggcggta cgctggttta ctcgacctat
    15361 accttaaacc gggaagaaaa cgaagccgtt tgccggtggc tgaaagagac ttaccccgac
    15421 gaagtagagt ttttaccact tggcgatctc ttccctggtg caaacaaggc gctgaccgaa
    15481 gaaggttttt tgcatgtttt cccacaaatt tacgactgcg aaggcttctt cgttgctcgt
    15541 ctgcgtaaaa ctcaggcgat ccccgcctta cccgccccaa aatacaaagt gggcaatttc
    15601 ccgttcagcc cggtgaaaga tcgcgaagcc ggacaaattc gtcaggcggc tgcaagtgtt
    15661 ggcttaaact gggatggaaa cctgcgactc tggcaacgcg acaaagaact gtggttgttc
    15721 ccggtaggca ttgaagccct gatcggtaaa gtccgatttt ctcgcttagg gattaaactt
    15781 gccgaaacgc ataacaaagg ttatcgctgg cagcatgaag cggttattgc ccatgcctcc
    15841 cccgacaatg taaacgcttt tgaactgaca ccgcaggaag cgtaggagtg gtatcgcggg
    15901 cgcgatgttt acccgcaagc cgcgccagtg gcggatgacg tgttggttac tttccagcat
    15961 cagccgattg gtttagccaa acggattggt tcgcgactga aaaacagcta cccgcgtgaa
    16021 ctggtgcggg acgggaaact ttttaccagt aacgcatgac agcgcacaaa aaaagcgcac
    16081 tttttgactg gcacattcgc cggcctcaac taggctgaaa aattgtgcga tcggactggt
    16141 cgtactacaa tcagcagcta aatggagagc acgaagatga aaaccagtgt gcgcataggc
    16201 gcttttgaaa tcgacgacgg cgaattacac ggtgaatcgc cgggtgatcg aacgttaacc
    16261 attccttgta aatctgaccc cgatttatgt atgcaactgg atgcctggga tgctgaaacc
    16321 agtatccctg ccctgctaaa tggcgaacac tctgtccttt accgtacccg ttacgatcaa
    16381 caatctgatg cctggattat gcgtcttgcc tgatccaaaa agaacccgtc ggcatggcgg
    16441 gttatttgtc ctgg
//
LOCUS       DAJTYL010000091        15726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYL010000091 DAJTYL010000000
VERSION     DAJTYL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.091"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO572_002866"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO572_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6661799.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO572_002867"
     CDS             complement(1614..1937)
                     /locus_tag="OO572_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6661800.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO572_002868"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO572_002868"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6661801.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO572_002869"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO572_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6661802.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO572_002870"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO572_002870"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6661803.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO572_002871"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO572_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6661804.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO572_002872"
     CDS             7537..8574
                     /locus_tag="OO572_002872"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6661805.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO572_002873"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO572_002873"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6661806.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO572_002874"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO572_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6661807.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO572_002875"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO572_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6661808.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO572_002876"
     CDS             10745..11626
                     /locus_tag="OO572_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6661809.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO572_002877"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO572_002877"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6661810.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO572_002878"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO572_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6661811.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO572_002879"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO572_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
BASE COUNT         3799 a         3973 c         4108 g         3846 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagata
//
LOCUS       DAJTYL010000092        15410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYL010000092 DAJTYL010000000
VERSION     DAJTYL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.092"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1175)
                     /locus_tag="OO572_002880"
     CDS             complement(<1..1175)
                     /locus_tag="OO572_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6661812.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO572_002881"
     tRNA            complement(1374..1458)
                     /locus_tag="OO572_002881"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO572_002882"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO572_002882"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6661813.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO572_002883"
     CDS             2802..4304
                     /locus_tag="OO572_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6661814.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO572_002884"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO572_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6661815.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO572_002885"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO572_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6661816.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO572_002886"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO572_002886"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6661817.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO572_002887"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO572_002887"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6661818.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO572_002888"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO572_002888"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6661819.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO572_002889"
     CDS             complement(11869..13065)
                     /locus_tag="OO572_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6661820.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO572_002890"
     CDS             13258..13761
                     /locus_tag="OO572_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6661821.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO572_002891"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO572_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6661822.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OO572_002892"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OO572_002892"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6661823.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVEALAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3932 c         3969 g         3533 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgagttt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaag cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTYL010000093        15101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYL010000093 DAJTYL010000000
VERSION     DAJTYL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.093"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1302)
                     /gene="speA"
                     /locus_tag="OO572_002893"
     CDS             complement(<1..1302)
                     /gene="speA"
                     /locus_tag="OO572_002893"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6661824.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIG"
     gene            complement(1311..1442)
                     /gene="yqgB"
                     /locus_tag="OO572_002894"
     CDS             complement(1311..1442)
                     /gene="yqgB"
                     /locus_tag="OO572_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6661825.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1578..1793
                     /locus_tag="OO572_002895"
     CDS             1578..1793
                     /locus_tag="OO572_002895"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661826.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2097..3251
                     /gene="metK"
                     /locus_tag="OO572_002896"
     CDS             2097..3251
                     /gene="metK"
                     /locus_tag="OO572_002896"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6661827.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            3688..5082
                     /gene="galP"
                     /locus_tag="OO572_002897"
     CDS             3688..5082
                     /gene="galP"
                     /locus_tag="OO572_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6661828.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5159..5656
                     /locus_tag="OO572_002898"
     CDS             5159..5656
                     /locus_tag="OO572_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6661829.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            5751..6458
                     /gene="endA"
                     /locus_tag="OO572_002899"
     CDS             5751..6458
                     /gene="endA"
                     /locus_tag="OO572_002899"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6661830.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6538..7269
                     /gene="rsmE"
                     /locus_tag="OO572_002900"
     CDS             6538..7269
                     /gene="rsmE"
                     /locus_tag="OO572_002900"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6661831.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7282..8232
                     /gene="gshB"
                     /locus_tag="OO572_002901"
     CDS             7282..8232
                     /gene="gshB"
                     /locus_tag="OO572_002901"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6661832.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8341..8904
                     /locus_tag="OO572_002902"
     CDS             8341..8904
                     /locus_tag="OO572_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6661833.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            8904..9320
                     /gene="ruvX"
                     /locus_tag="OO572_002903"
     CDS             8904..9320
                     /gene="ruvX"
                     /locus_tag="OO572_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6661834.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARFGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9496..10476)
                     /locus_tag="OO572_002904"
     CDS             complement(9496..10476)
                     /locus_tag="OO572_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6661835.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            10494..11198
                     /gene="yggS"
                     /locus_tag="OO572_002905"
     CDS             10494..11198
                     /gene="yggS"
                     /locus_tag="OO572_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6661836.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11216..11782
                     /gene="yggT"
                     /locus_tag="OO572_002906"
     CDS             11216..11782
                     /gene="yggT"
                     /locus_tag="OO572_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6661837.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            11779..12069
                     /locus_tag="OO572_002907"
     CDS             11779..12069
                     /locus_tag="OO572_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6661838.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12077..12670
                     /locus_tag="OO572_002908"
     CDS             12077..12670
                     /locus_tag="OO572_002908"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6661839.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            12663..13799
                     /gene="hemW"
                     /locus_tag="OO572_002909"
     CDS             12663..13799
                     /gene="hemW"
                     /locus_tag="OO572_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6661840.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14113..15076
                     /locus_tag="OO572_002910"
                     /pseudo
     CDS             14113..15076
                     /locus_tag="OO572_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT         3728 a         3912 c         3832 g         3629 t
ORIGIN      
        1 gccgatatga atgtcgtgca gatccatctg gctgtcgtgt aaccattcac gcaaagaacg
       61 gcgagttccc ggttcgtgca tttcctgcca ggtttcccac atgctttgca gcgcacgcgg
      121 cgcatcttct acgggcgcgg tcggcaccgt atattcgtta cgttccacgc cgatgatatt
      181 cgacaccagc acggtatggt gcgcagtcac cgcacggccc gattcggtga ttaccgtcgg
      241 atgcggcaga ccattttctt cacacgcatc gccaatcgcc cagatgatgt tgttggcgta
      301 ttcattgagg ccatagttta cggagcagtc agactgcgaa cgggtgcctt cataatccac
      361 gcccagaccg ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac
      421 atagaaacgc gcggattcac gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga
      481 accgaggtgg aagtgcagta gttgcaggct gtcgagacgc ccggcttcac gcagggtttc
      541 aaccagttgc agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggagga
      601 ctgccattta cccgagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac
      661 attcagacgt tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag
      721 atagaccttg tgccccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt
      781 ataaccgttg cagacgatga cgctacgagt catgccagca tgtgccagca ctgccatcaa
      841 ctcggctttg gaaccggctt ccagacccag cggttcaccc gaatgaatca gggactcgat
      901 cacgcggcgg tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc
      961 gtaggattcc ctcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat
     1021 ctgtgggaaa cagaacagtg caggcagacg ctgaccctgt gcttcacgag ttttcactaa
     1081 ctgcgcgaga tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc
     1141 cagctcgtta acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat
     1201 cttgctggct tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc
     1261 gcccgctgac gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt
     1321 ttattaagtg taaaacagtt aacgactgtc gcagcctgga actgcgataa caacccataa
     1381 gcacatggat tttccagcag tgtatgctga cgctccgact gcgcaaccgg tttctttttc
     1441 ataacattat taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa
     1501 actcttgttt ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa
     1561 tcctgagaag cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt
     1621 gcggggaacg agtcttcacc agaacggtga gacaggttaa gcagcagaca acggttcatt
     1681 atttcgtatc acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt
     1741 caacccggct ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc
     1801 gccgcgtttt ataccccaca atggcagaaa attgcaaaag ataaatacgc agaatgccgg
     1861 cattgtcagg aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa
     1921 gtggaaatcg ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc
     1981 gattaacact cagactgcca gtgtttttaa cctgcagagt cgtggtagga tccgctacca
     2041 cagaaaatcc acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg
     2101 caaaacacct ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc
     2161 aaatctctga tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt
     2221 gcgaaaccta cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct
     2281 gggtagacat cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg
     2341 acatgggctt tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg
     2401 acatcaacca aggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc
     2461 tgatgtttgg ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg
     2521 cacaccgtct ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc
     2581 gcccggacgc gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtattg
     2641 atgctgtcgt gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag
     2701 cggtaatgga agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca
     2761 aattcttcat caacccgacc ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc
     2821 tgactggccg taaaattatc gttgatacct acggcggcat ggcgcgtcac ggtggcggtg
     2881 cattctctgg taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg
     2941 tcgcgaaaaa catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg
     3001 caatcggcgt ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc
     3061 cttctgaaca actgactctg ctggtacgtg agttcttcga cctgcgccca tacggactga
     3121 ttcagatgct ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg
     3181 gtcgtgaaca tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg
     3241 gtctgaagta atctttcttc acctgcgttc aaaggccagc ctcgcgctgg cctttttctt
     3301 ttacctcacc gcggtggata ggcgttcacg ccgcatccgg caaaaaaact gcccgcacaa
     3361 taacatcatt cttcctgatc acgtttcacg gcaaattacc atcacaactg aaaccgatta
     3421 caccaaccac accagcaaaa tatttgtaat attttcatat tattattcag ctttcacagt
     3481 tgttacattt cttttcagta aagtcttaat tgcagatgac agcgtttaat ctatgatgat
     3541 ataactcaat tattttcatg cccttaaatc ataactaaga taaatgttag tgtaagcgat
     3601 tacactgatg tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa
     3661 taaaaaataa ctatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac
     3721 aaggcaatga cgtttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg
     3781 gatatcggtg taattgctgg cgcactgccg tttattgcag atgaattcca gataacttcg
     3841 cacacgcaag aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc
     3901 atcggctggc tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg
     3961 tttgttgccg gttcactgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcg
     4021 cgcgttctgc tggggctggc ggtgggtgtg gcctcttata ccgcaccact gtacctgtca
     4081 gaaattgcgc cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt gatgatcact
     4141 atcgggatcc tcggtgctta tctttctgat accgccttca gctacaccgg tgcatggcgc
     4201 tggatgctgg gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg
     4261 ccagacagcc cacgttggtt tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg
     4321 ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt
     4381 ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga atagcaactt ccgccgcgcg
     4441 gtgttccttg gcgtactgtt acaggtaatg cagcaattca ccgggatgaa cgtcatcatg
     4501 tattacgcgc cgaaaatctt cgaactggcg ggttatacca acaccaccga gcaaatgtgg
     4561 gggacagtga ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt
     4621 gaccgctggg gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggcatg
     4681 ggcgtactcg gtacaatgat gcatatcggt atccactctc cgtcggcgca gtatttcgcc
     4741 atcgccatgc tgctgatgtt tattgtcggt tttgccatga gtgccggtcc gctgatttgg
     4801 gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc
     4861 gccaccaact ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg
     4921 ctgggtaacg ccaacacctt ctgggtgtat gcggctctga acgtactgtt tatcctgctg
     4981 acattgtggc tggtaccgga aaccaaacac gtttcgctgg aacatattga acgtaatctg
     5041 atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt aatctcctca ggcttcctcc
     5101 cgtcgcggag gaagccgcct cttgcagtca tcttttcttc gctctatcct ctgccgctat
     5161 gaaaacatcc cgtctcccta tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa
     5221 actcgcccag gccaacctga agctagggcg taactacccg gagccaaaac tctcttacac
     5281 ccagcgcgga acctccgccg gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc
     5341 cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc acgaactggc
     5401 acatttgctg gtatggaaac atttcggccg cgtagcgcca catggcaaag agtggaagtg
     5461 gatgatggaa agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc
     5521 cgtgcgtcgc aacaccttcc cctaccgctg taagtgccag gaacatcagc ttaccgtacg
     5581 ccgccataat cgcgtagttc gtggcgaggc catctatcgc tgtgttcact gcggtgaaca
     5641 gctggttgcg aaataaccac ctgaactatc aggaactttc ctgatctggc tgattgcata
     5701 ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt
     5761 atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccgacg ttggccgaag
     5821 gtatcaatag tttttctcag gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg
     5881 gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc
     5941 aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac
     6001 acgtcgttcc cgcctggcaa ttcggtcacc agcgccagtg ctggcaggac ggtggacgta
     6061 aaaactgcgc taaagatccg gtctatcgca agatggaaag cgatatgcat aacctgcagc
     6121 cgtcagtcgg cgaggtgaat ggcgatcgcg gcaattttat gtacagccag tggaatggcg
     6181 gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gctgccgaac
     6241 caccagcgcg tgcacgcggt gccattgcgc gcacctactt ctatatgcgc gaccaataca
     6301 acctgacact ctctcgccag caaacgcagc tgttcaacgc atggaacaag atgtatccgg
     6361 ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc
     6421 cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggattctttt
     6481 tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg
     6541 cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc
     6601 gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa
     6661 ttgtttgacg gtagcaacca ggtctttgac gccgaaatta ccagcgccag caaaaaaagc
     6721 gtggaagtga aggtgctgga aggccagatc gacgatcgcg aatctccgct gcatattcac
     6781 ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta ctatccagaa atcgatcgaa
     6841 ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt
     6901 gaacgtctga acaagaagct tcagcagtgg cagaagattg caattgctgc ctgtgagcag
     6961 tgtggtcgta accgggtgcc ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca
     7021 gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg
     7081 ttgcctttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca
     7141 gatgaaattg ccatgactgc ccgctatcaa tttactgata tcctgttggg acctcgcgtt
     7201 ttgcgtacag agacaactgc gctcaccgcc attaccgcgc tacaagtacg atttggcgat
     7261 ttgggctaac ggagaagaat aatgatcaag ctcggcatcg tgatggaccc catcgcaaac
     7321 atcaacatca agaaagattc cagtttcgct atgttgctgg aagcacagcg tcgtggttac
     7381 gaacttcact atatggagat gggcgatctg tatctgatca atggcgaagc ccgcgcccat
     7441 acccgcacgc tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt cggtgaacag
     7501 gatctgccgc tggccgatct cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc
     7561 gagtttatct acgcgaccta tattctggaa cgtgccgaag agaaagggac gctgatcgtt
     7621 aacaagccgc agagcctgcg cgactgtaac gagaaactgt ttaccgcctg gttctctgac
     7681 ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa
     7741 cacagcgaca tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg
     7801 aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc tgactgagca tggcacttgc
     7861 tactgcatgg cgcaaaatta cctgccagcc attaaagatg gcgacaaacg cgtgctggtg
     7921 gtggatggcg agccggtacc gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt
     7981 ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa
     8041 atcgcccgtc agatcgggcc gacgctgaaa gaaaaagggc tgatttttgt tggtctggat
     8101 atcatcggcg accgtctgac tgaaattaac gtcaccagcc caacctgtat tcgtgagatt
     8161 gaagcagagt ttccggtgtc gatcaccgga atgttaatgg atgccatcga agcacgttta
     8221 cagcagcagt aacccacctt agcgagaagg atctcgttga gactctgagt gacagcgccc
     8281 ttctttccac gcatactggg cgctgttgct tttttgaacc aggaaacaga acctctgaca
     8341 atgaatttac agcatcactt tcttattgcc atgcctgctc tccaggatcc gattttccgt
     8401 cgttccgtgg tctacatttg cgaacataat accaatggtg caatggggat catcgtcaac
     8461 aagccgctgg aaaatctcaa aattgaaggg attctggaaa agctgaagat cacgccggag
     8521 ccgcgtgatg aatcaatccg tctggataaa ccggttatgc tcggcggccc actggctgaa
     8581 gatcgcgggt ttattttgca tactccgccc tccaattttg cttccagcat tcgcatttca
     8641 gacaacacgg tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa
     8701 ccgtctgacg tattggtggc tctgggctat gcctcctggg aaaaaggtca actggaacaa
     8761 gaaattctcg ataacgcgtg gctaacggcc ccggcggatc tgaatattct gttcaaaacg
     8821 ccgattgccg accgctggcg cgaggcggca aaactgattg gtgtggatat tctcaccatg
     8881 cctggtgtgg caggacacgc ctgatgagtg gaaccttact cgccttcgac ttcggcacca
     8941 aaagcattgg cgtagcggtc ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa
     9001 ttaaagcgca ggacggtacg ccggactgga acctcatcga acgtttactg aaagagtggc
     9061 agcctgacga aatcatcgtc ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa
     9121 ccgccagagc gcgcaaattt gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc
     9181 tccatgacga gcgtcttagc actgtggaag cccgtttcgg tctttttgaa cagggcggct
     9241 atcgggcgct caacaaaggc aaagttgact ctgcctctgc ggttattatt ctcgaaagct
     9301 atttcgagca gggatattaa ggcgatctaa aagcctggcg gatagtcgtt accgccatct
     9361 tgccggatgc ggcgtgaacg ccttatccag cacacatctg gcagcggctg taggtctgat
     9421 aagacgcgtt agcgtcgcat cagacattta ttgcctttat aggccctata aacttgcgca
     9481 tcgggcatgg caacgtcaca aacgcccttc ccccacccgc tgctgataac tctgctgaaa
     9541 cgttaacatc cccaccttct gcccggtttg aataacatgc ggtaactggt gggttttccc
     9601 ttcgcgaatc aaattcccca ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg
     9661 tccttcctgt ttatccactt ccagcttttg tgacagcact gcccgtaaac tacccgcaag
     9721 ttgattacgt acgggatctt tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc
     9781 ctgcgcggca ccacgtgtat gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt
     9841 cagcgccaga cggattgtct cactgtcacg tagctctccg agcaaaatca catcaggatc
     9901 ttcccgcaat gcagcccgca accccgatgc gaacgtcata cagtgcaaac caatttcccg
     9961 ctgctggatc aaacatcgct ggctggcata gagatattcc acaggatctt ccagcgtcag
    10021 aatatgcgca tcggcatgtt gattaagata gccaaccatc gccgccagcg tggtagattt
    10081 gccactcccc gtagcccccg tcaccagaat caggccattc tcgctcttga gtaattccgg
    10141 caataccggt ggtgcaccaa gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa
    10201 tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc
    10261 cagcgacacg gcaaaatcca gctggccatt ttccagcaat attgcccgct gatcgtcatc
    10321 cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag cttccattct
    10381 gccacgaatg cgccatcgtg cgggccaggc gctgcacagg tgtagatccg agacgttatg
    10441 ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg aaaatgaacg
    10501 atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca acgcgttgcg
    10561 gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca
    10621 tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt caggaagggg
    10681 tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg cattttattg
    10741 gcccgttgca gtctaataaa agccgcctag tggcagagca tttcgactgg tgtcatacca
    10801 tcgaccgttt gcgcattgct acccgcctca acgagcagcg cccggcagaa cttccccctc
    10861 ttaacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg attcaactgg
    10921 cagaactgga cgagctggca gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc
    10981 tgatggcaat ccctgcgctt gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa
    11041 tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg ctctctctgg
    11101 gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt cgtatcggca
    11161 ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga acgccatgaa
    11221 tacgttgact ttcctgcttt caacggtcat tgagctgtat acaatggtgc tgttattacg
    11281 catctggatg cagtgtgctc attgtgattt ttacaccccc ttctcacagt ttgtagtgaa
    11341 ggtagcgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg gtcccattga
    11401 cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccattg tgctgtttga
    11461 agtggtgacc ttcctgccaa tcatctggat tgccggttta ctgattctgc tgaaaaccat
    11521 cggcttgctg attttctggg tcctgctggt gatggcgatt atgagctggg taagccaggg
    11581 tcgtagcccg attgaatacg tgctgattca gctggccgat ccgctgctgc gcccgattcg
    11641 ccgcttgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg ttctgctgct
    11701 gtatgccatc aatatgggtg tcgcagaagt attacaggca accggaaaca tgctgctgcc
    11761 ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct ggttttacgg
    11821 ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg cgacgaagtt
    11881 aaagtcgcca ttaccgcgcc gccggttgac ggccaggcca acagtcatct ggtgaagttt
    11941 ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc
    12001 cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat cgcggcgtta
    12061 attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg
    12121 tgagctggcg tcgctgctta gcgactttgg tcttgatatc gtggcccaaa cagacctcgg
    12181 cgttgattcc gctgaagaaa ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg
    12241 ccatgcggca aaagtgaccg gtttaccggc aattgccgac gactctggtc tggcggtaga
    12301 tgcgctgggt ggcgcgccgg gtatttactc cgcgcgttat tccggtgaag acgcgaccga
    12361 tcaaaagaat ctgcaaaaac tgttggaaac actgaaagac gtaccggacg accaacgtca
    12421 ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg gaagatccga ctccgctggt
    12481 atgccacggc agctggccgg gcgtgattac tcgcgaacca gcgggcactg gtggctttgg
    12541 ttatgatcca atcttcttcg taccttccga agggaaaacc gccgccgaac tgacccgcga
    12601 agaaaagagc gccatttccc accgtggtca ggcgttgaaa ctgctgctgg acgctttacg
    12661 taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc gtgcagaaat
    12721 gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac gacgattatg
    12781 ttcagcatct gctttgcgat ctggacaatg atgtggctta cgctcagggc cgtgaagtaa
    12841 agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg atgcaaacgc
    12901 tgctagacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt actatggaag
    12961 cgaacccagg cacggtagaa gcggatcgct ttgtcgatta tcagcgtgct ggtgtaaacc
    13021 gcatctctat tggtgtgcag agttttagcg aagaaaagct gaaacgactt gggcgtattc
    13081 atggcccgca agaagcgaaa cgagcggcaa agctggcgag cggtttaggg ttacgtagct
    13141 ttaaccttga tttgatgcat ggactaccgg atcaatcact ggaagaggcg cttggcgatc
    13201 tacgccaggc cattgaactg aatccgccgc atctttcctg gtatcaactg actatcgaac
    13261 ctaatacgct gtttggctcg cgaccgccgg tgctgccgga cgatgacgcg ctgtgggata
    13321 ttttcgaaca ggggcatcag ttattaaccg cagcgggtta tcagcaatat gaaacttccg
    13381 cttacgccaa acccggttat cagtgccagc acaatctcaa ctactggcgc tttggtgact
    13441 acatcggtat tggctgcggc gcgcacggca aagtgacctt cccggatggg cgcattctgc
    13501 gtaccactaa aacgcgtcat cctcgtggtt ttatgcaagg aaggtatctg gaaagccagc
    13561 gtgatgtcga agccgcagat aagccgtttg agttctttat gaatcgcttc cgtctgctgg
    13621 aggccgcgcc gcgcgtggag tttattgcgt ataccgggct ttgcgaagat gtgattcgcc
    13681 cacagttaga cgaggcgatt gcccagggtt atctcaccga atgtgcggat tactggcaga
    13741 taacggaaca tggtaaatta ttcttaaact cacttcttga attattcctc gctgagtaat
    13801 cattaatgcg cctgcgggcg cattaatcac ttcttttatc cccccgttaa ttaacctcgg
    13861 cagaaatata tttcttactg aataaatatc gacgacattt attatatata tttagtgaat
    13921 atttactaat cattcattga ttataaatat gtacaacctg gcgcacaaat ttaatattta
    13981 atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagaca ggttaaccaa
    14041 tttgtcgcac aaatcactga taagcacaat tagatatcaa ccaatttcca tccttcaaca
    14101 gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc atccttgctg
    14161 gctctggtta gcactggcgt aaacgccgca gaaaaagtgg cgttaaagtt agcatacaac
    14221 ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga ggtaaaacag
    14281 ctgtcaaaag gtaatatggt gatccgtatt tatcccagca gccagatggg taatgcgcga
    14341 gaaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc cgccagtgat
    14401 ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt aagttgtttt
    14461 tggtgaagtt ggtaaagaaa ttatggactc aacaaaagac aaaggctttt ttgctttatc
    14521 agcttatgta gcgggtactc gcagtttcta tgcgaaaaaa ccgattacaa agccagaaga
    14581 tcttaaagga ctgaaaattc gtgttcagcc aagtccgacc accatcaaaa tgattgaact
    14641 gatgggtggt tcacccactc caatttcatt tggcgaagtt tatactgcga tgcagcaagg
    14701 tgtagtagat ggcgcagaaa ataacgtgcc ttcctggatg cagactcgac atattgaaat
    14761 tgccaaagtt ttttctgaag atgaacacgc ctcaattcct gactttttgg ttatctccac
    14821 caaaacatgg aataaattaa ccccagaaca gcaacaaatc ctcgaaacag cggcgaagaa
    14881 atccgaagct tatcaacaaa aattgtggga aaaaattgac gctgacacac gtgctcaggc
    14941 gaaagctatg gggggtgaaa ttgtaaaagt ggataaagcg ccattccgta aagccgtgca
    15001 acctctgttt gacgacttca aaaaagatcc aaaacaagct gctttgttag agaaattcga
    15061 caacgctgct caataatatt ccgggccagc ctctggccct g
//
LOCUS       DAJTYL010000094        15025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYL010000094 DAJTYL010000000
VERSION     DAJTYL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.094"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO572_002911"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO572_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6661841.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO572_002912"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO572_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6661842.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO572_002913"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO572_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO572_002914"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO572_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6661843.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO572_002915"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO572_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6661844.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO572_002916"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO572_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6661845.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO572_002917"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO572_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6661846.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO572_002918"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO572_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6661847.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO572_002919"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO572_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6661848.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO572_002920"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO572_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6661849.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO572_002921"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO572_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6661850.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO572_002922"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO572_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6661851.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO572_002923"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO572_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6661852.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO572_002924"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO572_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6661853.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO572_002925"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO572_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6661854.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO572_002926"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO572_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6661855.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO572_002927"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO572_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6661856.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO572_002928"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO572_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6661857.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO572_002929"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO572_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6661858.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO572_002930"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO572_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6661859.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO572_002931"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO572_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6661860.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO572_002932"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO572_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6661861.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO572_002933"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO572_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6661862.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO572_002934"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO572_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6661863.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO572_002935"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO572_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6661864.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO572_002936"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO572_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6661865.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO572_002937"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO572_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6661866.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO572_002938"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO572_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6661867.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO572_002939"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO572_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6661868.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO572_002940"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO572_002940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6661869.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
BASE COUNT         3884 a         3518 c         4001 g         3622 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcggcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatca
//
LOCUS       DAJTYL010000095        14883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYL010000095 DAJTYL010000000
VERSION     DAJTYL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.095"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..486
                     /locus_tag="OO572_002941"
     CDS             <1..486
                     /locus_tag="OO572_002941"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6661870.1"
                     /translation="RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHC
                     YTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVE
                     IERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTP
                     A"
     gene            501..797
                     /gene="citD"
                     /locus_tag="OO572_002942"
     CDS             501..797
                     /gene="citD"
                     /locus_tag="OO572_002942"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6661871.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            794..1702
                     /gene="citE"
                     /locus_tag="OO572_002943"
     CDS             794..1702
                     /gene="citE"
                     /locus_tag="OO572_002943"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6661872.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            1713..3245
                     /gene="citF"
                     /locus_tag="OO572_002944"
     CDS             1713..3245
                     /gene="citF"
                     /locus_tag="OO572_002944"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6661873.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3249..3799
                     /gene="citX"
                     /locus_tag="OO572_002945"
                     /pseudo
     CDS             3249..3799
                     /gene="citX"
                     /locus_tag="OO572_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            3774..4652
                     /gene="citG"
                     /locus_tag="OO572_002946"
     CDS             3774..4652
                     /gene="citG"
                     /locus_tag="OO572_002946"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6661874.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            4703..6165
                     /gene="citT"
                     /locus_tag="OO572_002947"
                     /pseudo
     CDS             4703..6165
                     /gene="citT"
                     /locus_tag="OO572_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            6279..7085
                     /gene="rna"
                     /locus_tag="OO572_002948"
     CDS             6279..7085
                     /gene="rna"
                     /locus_tag="OO572_002948"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6661875.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7314..7724
                     /gene="rnk"
                     /locus_tag="OO572_002949"
     CDS             7314..7724
                     /gene="rnk"
                     /locus_tag="OO572_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6661876.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(7955..9164)
                     /locus_tag="OO572_002950"
                     /pseudo
     CDS             complement(7955..9164)
                     /locus_tag="OO572_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            9385..9813
                     /gene="uspG"
                     /locus_tag="OO572_002951"
     CDS             9385..9813
                     /gene="uspG"
                     /locus_tag="OO572_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6661877.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9934..11499)
                     /gene="ahpF"
                     /locus_tag="OO572_002952"
     CDS             complement(9934..11499)
                     /gene="ahpF"
                     /locus_tag="OO572_002952"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6661878.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11744..12307)
                     /gene="ahpC"
                     /locus_tag="OO572_002953"
     CDS             complement(11744..12307)
                     /gene="ahpC"
                     /locus_tag="OO572_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6661879.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12679..13425
                     /gene="dsbG"
                     /locus_tag="OO572_002954"
     CDS             12679..13425
                     /gene="dsbG"
                     /locus_tag="OO572_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6661880.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            13635..14537
                     /locus_tag="OO572_002955"
     CDS             13635..14537
                     /locus_tag="OO572_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6661881.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            14684..14883
                     /locus_tag="OO572_002956"
                     /pseudo
     CDS             14684..14883
                     /locus_tag="OO572_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3622 a         3885 c         3651 g         3725 t
ORIGIN      
        1 cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc
       61 tcagaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc
      121 attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg
      181 cttggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac
      241 aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcagcgcc catcgaactg
      301 gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa
      361 ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat
      421 tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc
      481 gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt
      541 ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca
      601 atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg
      661 cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca
      721 ttttacatgc acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat
      781 gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag
      841 catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca tctacccggc
      901 tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg
      961 ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg
     1021 tgtcaacgcg ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg
     1081 tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atattgaaaa
     1141 agagatcctg cgtatagaaa aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc
     1201 ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg cttccgagcg
     1261 tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc
     1321 cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc
     1381 gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca
     1441 agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca
     1501 gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccaccg
     1561 cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg
     1621 caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact
     1681 ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac
     1741 gacaagaacg ggtagcggcc tggaatcgtc gcactgaatg cgatcttgcc gctttccaga
     1801 actcaccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc
     1861 tggaagaagc gattcgtcgc tcaggtttac aggacggcat gacggtttcc ttccatcacg
     1921 ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct
     1981 ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg ctggtagaac
     2041 acattcgcca gggcgtggtt acccgcattt atacctctgg cctacgcggt ccactggcgg
     2101 aagagatctc ccgtggtctg ctggcggaac cggtgcagat ccactctcac ggcggtcgtg
     2161 tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc gtcccgtcct
     2221 gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct
     2281 atgcaatggt tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc
     2341 cttatccgca taatccggca agcattgagc aagatcaggt tgatttgatc gtcaaagtta
     2401 atcgcgttgg cgatgctgca aaaatcggcg ctggtactac ccgtatgacc actaacccgc
     2461 gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactctggc tacttcaaag
     2521 aaggtttctc catgcaaacc ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg
     2581 aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcga
     2641 cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg
     2701 acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc
     2761 agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga
     2821 gcgcgctgga aattgacagg cagttcaacg ttaacgtgct gaccggttct gacggcgtac
     2881 tgcgtggcgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg
     2941 tcgcgccact ggtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca
     3001 ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac
     3061 gtccggaact agcagaacgt ctgcaggaag cgggcattaa agtggtttcc attgagtggc
     3121 tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg
     3181 tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg
     3241 aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc
     3301 tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag caccatcctg
     3361 ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac
     3421 gccgaatttt taatcatcgc gtgacagcct tgcgtgcctt agccacaaag cagggctggc
     3481 aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgagcattg
     3541 ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg
     3601 ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact
     3661 attcactgcg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     3721 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     3781 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     3841 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     3901 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     3961 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     4021 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     4081 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     4141 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     4201 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     4261 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     4321 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     4381 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     4441 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     4501 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     4561 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     4621 tgatccttac ctgtttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     4681 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     4741 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     4801 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     4861 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     4921 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     4981 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     5041 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     5101 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     5161 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     5221 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     5281 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     5341 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     5401 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     5461 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     5521 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     5581 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     5641 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     5701 cgctataaca gcgcatggaa cacgctggtc aacctggcag ctctggttgt gatggctaac
     5761 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacctggaa
     5821 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     5881 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     5941 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     6001 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     6061 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     6121 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     6181 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     6241 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     6301 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     6361 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     6421 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     6481 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     6541 ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     6601 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     6661 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     6721 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     6781 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     6841 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     6901 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     6961 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     7021 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     7081 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     7141 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     7201 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     7261 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     7321 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     7381 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     7441 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     7501 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     7561 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     7621 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     7681 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     7741 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     7801 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     7861 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     7921 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     7981 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     8041 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     8101 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     8161 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt
     8221 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     8281 taacaatgcc gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt
     8341 tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg
     8401 tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc
     8461 gggatcgcgc cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca
     8521 aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cacaggacca
     8581 gcaccataga ctgcaacgct tgaaccttgt tggatctgca cattttttgc tgcctgccat
     8641 gccgttggca gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact
     8701 ttaaacggcc ccacattccc tttcgggacg cggacatatt ccgcctgccc gccaggaacg
     8761 ccaccataca ggtgactata accaaacaat gccgcgggag caggtatctg ttttttattg
     8821 agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg taatcgacag
     8881 aaaaaacagt cgccacaagc aatgacgaac ggaattacca cccggtcgcc tttttgcaaa
     8941 ttttttacgt ccttcccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg
     9001 ccatgtttaa cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc
     9061 gttaccgtaa tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt
     9121 tctacctgaa cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca
     9181 gttcaaaaaa gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc
     9241 aggttttggg agaattccta gtgttcagtg agaatatatt aatcacagaa accgttcgta
     9301 aggaaaaaga accagacaaa tatcagggat aaatattata atcatggaga atgatttgtt
     9361 catgattaac agggagaaag ggttatgtat aaggcaatca ttatgccagt tgatgtattt
     9421 gaaatggaat tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga
     9481 gttattcatc tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc
     9541 gctgatgtgc gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg
     9601 atggtcagcc acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc
     9661 gtgcgggatg aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttatcggt
     9721 tctcgcaacc catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc
     9781 cacgccaatc tgcctgtact ggtcgttcgc taacagataa aagtatttat gccgccctgc
     9841 ctggaacatc ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc
     9901 ggctaagcaa ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta
     9961 gtcaaaagca ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta
    10021 cggaaccgtc gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cacatttcgc
    10081 atcaatgata atctcgccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt
    10141 cggcagcaga ccaatctgga caaaaatacc ggccagttcg atgttgtgaa tattgccgct
    10201 gacacgatct cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt
    10261 ttgcgcattc agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg
    10321 gtcggctttc atttctggcg caaattccag cagcgttacg tgctcaacga tacccgccag
    10381 gtcgattgcc gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt
    10441 aaacagcggg ccgtcgcagt gcgggcagta ggtcacgcct ttagtgcgat actgatcttc
    10501 gcccggaacg ttcatgttgc gccattttgc acccgtcgcc acgataatgc tgcgtgcttt
    10561 cagtaccgcg ccagaagctg tttcaatctg gtgtagacca ccttcaaccg ctgccgggat
    10621 cagtttgctg gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt
    10681 cagtgcgcct gccaacttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc
    10741 aacggtatcg aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt
    10801 acgtgcggag taaattgctg ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc
    10861 ataagcatca cgcttgttca gctcttctgc cgcacgattt tccgcgccag tatcaatttt
    10921 ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac
    10981 tgccggaacg cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat
    11041 tgcagtgtgc ttgatgcgcg ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc
    11101 cgggcagtta tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg
    11161 gcgaatctgc tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagcaa
    11221 cgccagtacc agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggtccctg
    11281 gttggaacct gggttggtga tcaggaaaga cggcttacgc actggcaagc tgttatcttc
    11341 tttaaaggtg actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc
    11401 cgatttagcg ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag
    11461 gtaagccttg agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa
    11521 catcatcatg caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga
    11581 agaaaatcgg gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca
    11641 gagcaaggcg actagcctgt gaatccccgg gagcttacac aagtaagtga ccggggtgag
    11701 cgggcgacgc caacgccgct atggcgtgaa agacgaagga aatttagatt ttaccaacca
    11761 ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg caaacttcac
    11821 ctgggtgaga agctacgtac tgagctgctt tgattttacg cagcagatca gacgcgtcac
    11881 ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg tcaacaacga
    11941 aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg
    12001 cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca gagctgctgt
    12061 gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct acgcccagtt
    12121 tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa
    12181 agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt tcggtgattt
    12241 cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg gtgttaatca
    12301 aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat
    12361 tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct tcgtaagaca
    12421 actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc tttccaggtg
    12481 accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg
    12541 cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag ggcaatcacc
    12601 tgatctaagc tcttacctat gacagtgata ggttatgcct tttactcgac ttttgcactg
    12661 actgaaaaga acaaattaat gttaaaaaag atacttttac tggctctgct tcctgcaatc
    12721 gccttcgcag aggaacttcc ttccccagta aaagcgattg aaaaacaggg cattacaatc
    12781 atcaaaacgt tcgatgcccc cggaggaatg aaaggttatc tcggaaagta tcaggatatg
    12841 ggcgtcacca tctacctgac tcccgatggt aagcacgcta tctctggtta catgtacaac
    12901 gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc accagccgga
    12961 cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg
    13021 ccggtcattg tctacgtctt ctccgatccg ttctgcccat attgtaaaca gttctggcag
    13081 caggcgcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt
    13141 atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa
    13201 acctggcaac aatatgaagc ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta
    13261 agtacagagc aaatgaaagt gttaagtgac aatgagaaac tgatggacga tctgggggca
    13321 aatgtcacgc cggctatcta ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg
    13381 ttgcccgatc agaaaacgct taatatcatt atgggaaata aataaaaggt aattcatgga
    13441 tggttgaact atatccctgc ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac
    13501 cctaagtggc tgtgctattt tagaaaaact taatcctgag ttaagcaaaa aaacataatc
    13561 aataaaaata catacgcaaa aaacattgat taagtgaata taccatggaa gaaaaatata
    13621 aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta aatcttcttg
    13681 tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa tcactatata
    13741 tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt aatgaccctt
    13801 tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc
    13861 atcttgagaa aaacctcaaa ggccttgaac aaacaattaa tatagtcaat aagtccgaac
    13921 taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat aatagtatgc
    13981 tgatccgctg cctacggcaa gatgcctctg tcgagattga gtgccatgat atcctcatgt
    14041 cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg atcacccaaa
    14101 tgccagtgat cagccgttcc gtcatttgca tgccattgca tacaattcgc aatactctaa
    14161 tttgtagtaa taagcatccg cgaattactg acaatagcac ttatgaacaa attatggcag
    14221 aagagttcac ccaattaata gccaaatcag caggagttga tgatattcaa atggaaatag
    14281 acgagaagtt tatgaatcgc aaaatttcat ttcgtggttc ctctttgctg acaataatta
    14341 atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat aactaccacc
    14401 gtgactttct taatttaaaa gagataaaac tagaacatcc gttaccttca ataaagcttt
    14461 acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt attgaccgtc
    14521 taaatgagag tttttgatat ataactatag acagctacta taactttcat ctatttattc
    14581 acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata attaacagtc
    14641 ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat
    14701 tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctaga cactcaacac
    14761 cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct
    14821 gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcaactggga
    14881 ggc
//
LOCUS       DAJTYL010000096        14784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYL010000096 DAJTYL010000000
VERSION     DAJTYL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.096"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            157..840
                     /locus_tag="OO572_002957"
     CDS             157..840
                     /locus_tag="OO572_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6661882.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            884..5546
                     /locus_tag="OO572_002958"
                     /pseudo
     CDS             884..5546
                     /locus_tag="OO572_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5914..7569
                     /gene="lldP"
                     /locus_tag="OO572_002959"
     CDS             5914..7569
                     /gene="lldP"
                     /locus_tag="OO572_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6661883.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7569..8345
                     /gene="lldR"
                     /locus_tag="OO572_002960"
     CDS             7569..8345
                     /gene="lldR"
                     /locus_tag="OO572_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6661884.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8342..9532
                     /gene="lldD"
                     /locus_tag="OO572_002961"
     CDS             8342..9532
                     /gene="lldD"
                     /locus_tag="OO572_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6661885.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9728..10201
                     /gene="trmL"
                     /locus_tag="OO572_002962"
     CDS             9728..10201
                     /gene="trmL"
                     /locus_tag="OO572_002962"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6661886.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10254..11075)
                     /gene="cysE"
                     /locus_tag="OO572_002963"
     CDS             complement(10254..11075)
                     /gene="cysE"
                     /locus_tag="OO572_002963"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6661887.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11155..12174)
                     /gene="gpsA"
                     /locus_tag="OO572_002964"
     CDS             complement(11155..12174)
                     /gene="gpsA"
                     /locus_tag="OO572_002964"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6661888.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12174..12641)
                     /gene="secB"
                     /locus_tag="OO572_002965"
     CDS             complement(12174..12641)
                     /gene="secB"
                     /locus_tag="OO572_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6661889.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12704..12955)
                     /gene="grxC"
                     /locus_tag="OO572_002966"
     CDS             complement(12704..12955)
                     /gene="grxC"
                     /locus_tag="OO572_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6661890.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13097..13528)
                     /locus_tag="OO572_002967"
     CDS             complement(13097..13528)
                     /locus_tag="OO572_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6661891.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13773..>14784
                     /gene="gpmI"
                     /locus_tag="OO572_002968"
     CDS             13773..>14784
                     /gene="gpmI"
                     /locus_tag="OO572_002968"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HCR6661892.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKY"
BASE COUNT         3680 a         3824 c         3816 g         3464 t
ORIGIN      
        1 tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca
       61 tcactgtcat taatgtaaag caacatagct caccaataat acaatggaca actctaccgg
      121 cattgagcgc tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc
      181 agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc
      241 aagccagaaa gtagtttact gaaacaacaa gaagaacata ataataaaat tgtgctactt
      301 gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc
      361 ataataaaca gtacaaagac tttaactcac gtaattaaat ccgttaaaga ccaacaaaat
      421 acttttattt ttaccgagtt taacccagca aaaacgaagt atttcatcct gaacaacggt
      481 tcggtggctt tagcgggtcg agtgttatcc attgacgcca cagagaatgg tagtgttatt
      541 catatctcac tggtcaactt attaagtaca cctatctcaa acattggttt taatgcgaca
      601 tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca attacttttc
      661 aacacatcaa tgaaatccac attgaaatta ttaccaggtc aatggcaaga cattaatttg
      721 accctgaagg gtgtatcgcc caataacctg ggatatctga agttagccat caacatggaa
      781 aatattcagt tcgacaatct tccctctgct gaaaatcggc agaaaagaag caaaaaataa
      841 atgcagttag tacgcttata acaaggaata gcgagcatca ttaatgaaca aaatatttaa
      901 agttatctgg aaccctgcga cagggaatta tactgttacc agcgaaacgg caaaaagccg
      961 tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt
     1021 gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg
     1081 tagtggatca gctggcgacg gctgggttgc tataggcaaa ggggcgaaag caaatacttt
     1141 tatgaacacc agtggttcca gtactgctgt gggttatgac gctatagctg aaggccaata
     1201 tagctctgcc atcgggtcaa aaacccatgc gattggtggt gcatcaatgg cctttggggt
     1261 tagtgcaata tcagaaggcg atagaagtat agcactgggt gcctcttcgt attcattggg
     1321 ccaatactca atggccctcg gccgttattc aaaagcattg ggtaaattgt ctattgctat
     1381 gggggactct tccaaagcgg aaggagcaaa cgccattgcc ctggggcgta gcagtgtagc
     1441 tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag cgaacgctat
     1501 tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca ataatgcgaa
     1561 ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata aagtcaatac
     1621 tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca taggccaggg
     1681 caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt cgagaggctt
     1741 aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc ttgcgcttgg
     1801 tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt ccattgcgga
     1861 tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg ttaatgtgaa
     1921 aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc tttatgccat
     1981 tagcgactcg gtagcaaaaa aggcttggag gaggggctgc agtagatgtt gatgacggta
     2041 ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc
     2101 tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg
     2161 ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt
     2221 cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg
     2281 aagccaacac caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg
     2341 ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca
     2401 gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg
     2461 gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata
     2521 acacttccaa tattgccact aacaccacca acatctctaa cctgactgag acggtgacta
     2581 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg
     2641 gcaccgagac caccagcaaa ataaccaacg ttaaagatgg cgacctgacg actggcagca
     2701 ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca
     2761 ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2821 gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca
     2881 ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg
     2941 ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca
     3001 atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa
     3061 attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg
     3121 gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg
     3181 gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg
     3241 cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg
     3301 gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg
     3361 attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcctgac gacgctctgc
     3421 tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa
     3481 ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga
     3541 ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg
     3601 cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt
     3661 acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg tgggataaga
     3721 ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca
     3781 ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt
     3841 tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta
     3901 acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt cgtactaacg
     3961 acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca gctgtaggtt
     4021 ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc agcagcaccg
     4081 ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt gccaaaggtt
     4141 ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc actgacggcg
     4201 aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc atcaacgtag
     4261 ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac gctattggag
     4321 cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa gactcactgg
     4381 ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt aatgcaggta
     4441 ttggtatcgg tttgaacact ctggttctgg ctgatgcgat caacggcatt gctatcggca
     4501 gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct cagaccaccc
     4561 gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac tctgtaggtg
     4621 agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca gctggttcag
     4681 cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc gtagcgcaaa
     4741 atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta
     4801 atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac ttcaagacca
     4861 acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt
     4921 ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa
     4981 atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccagtg
     5041 ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggagtgc
     5101 gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca
     5161 acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga
     5221 actacgcgca gttgaagcaa agcgcgcagg aaacgaagca atacaccgat cagcggatgg
     5281 ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg
     5341 caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg
     5401 gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca
     5461 atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg
     5521 cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg
     5581 ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg tcaacaatct
     5641 aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc cccacaagaa
     5701 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5761 acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta tccctacaca
     5821 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
     5881 tacacgatgt gcgtggactc taggagacct gcaatgaatc tctggcaaca aaactacgat
     5941 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
     6001 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtagcaatc
     6061 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     6121 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
     6181 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6241 ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6301 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggcctg
     6361 ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca
     6421 tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat cgacagcttt
     6481 gagattggcc agatggtggg gcgtcagcta ccgtttatga ccattatcgt gctgttctgg
     6541 atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc ggtcgtggtt
     6601 gcaggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
     6661 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
     6721 caaccagttc gcgtattccg cttcggtgac ttgggggcgt cacaggttga tatgacgctg
     6781 gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg
     6841 accgctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt
     6901 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcaataaact ggttgcccgt
     6961 atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
     7021 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
     7081 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctacccatc
     7141 tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg actgtcatca
     7201 acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7261 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
     7321 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
     7381 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
     7441 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7501 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
     7561 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7621 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg
     7681 cgatgcaact cggcgtatca cgtaattcac tacgcgaggc gctggcaaaa ctggtgagcg
     7741 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
     7801 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
     7861 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
     7921 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
     7981 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     8041 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
     8101 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
     8161 aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa
     8221 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8281 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
     8341 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8401 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
     8461 agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg acttaagcct
     8521 ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac cggtgggttt
     8581 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
     8641 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
     8701 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
     8761 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
     8821 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg
     8881 ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     8941 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
     9001 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     9061 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
     9121 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9181 ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga
     9241 tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
     9301 gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg
     9361 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9421 gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc aggggctggg
     9481 taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc
     9541 gccccctcac cctaaccctc tccctcaggg agaggggacc gttcggcgct gtatgtactc
     9601 cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg ggagggccgg
     9661 ggtgagggga aaaggccgca cggttcacca attttctgct atcctgcccc cgcactaagg
     9721 gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata ctggcaacat
     9781 catccgtctt tgcgctaata ccggctttcg tctacatatc atcgaaccga tgggatttgc
     9841 ctgggacgac aagcgcctgc gccgcgcggg gctggactat cacgagttta ccgccgttac
     9901 gcgtcatcat gactatcgcg cgttcctcga agcagaaaat ccccagcgcc tgtttgccct
     9961 caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg attatctgat
    10021 gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc ccgctgaaca
    10081 aaaaattcgc attccgatgg tgccggacag ccgcagcatg aatctgtcca atgcggtgtc
    10141 ggtagtggtg tatgaagcct ggcggcagct gggatatccg ggagcgttgt tgagagatta
    10201 gttactgtat gccggataga tgattacatc gcatccggca cgatcacagg acattagatc
    10261 ccatccccat actcaaatgt atggttaata ccgttgaaat gctggtccat atccattgat
    10321 ggcttatcgc tgtctggttt accgacaata cgagccggaa cgccagcggc ggttgtatgc
    10381 ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg accaacttca
    10441 atattgccga ggattttcgc gcccgcgcca atcatcacac cttcacgaat tttcgggtga
    10501 cggtcaccac cagatttacc cgtaccgcca agcgtcacag attgcagaat cgatacgtcg
    10561 ttttcaatta ccgccgtttc acccacaacg atgcccgtgg cgtggtcgag cataatgcca
    10621 cggccaattt ttgctgccgg gtgaatatcg acctggaacg tcacagaaac ctggttttgc
    10681 agaaagattg ccaatgcgcg acgcccctga ttccacaacc agtgaccgat gcgataggcc
    10741 tgcaaggcat gaaaaccctt caggtataac aacggggttg agtatttatc gactgccggg
    10801 tcgcgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc cgggtcagcg
    10861 gcgtaggctt cttcaaccac ttcacggata gcaatagcag gcataattgg cgatgacagc
    10921 ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt cgtgcttgag tagcgtcgcg
    10981 tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc ggctttaata
    11041 ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg atgagataat
    11101 gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca aaggttccct ggggttagtg
    11161 actgctgcgc tcgtccttgc gtgcacgacc taataatgtc aatgctgcct cgcgcgcgtt
    11221 ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa cgccgaagcg
    11281 atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct gaccaatctt
    11341 ctcctgcgcg ctttgtacat ccatgccctg accgagcatc ataccaaaac ggcggttacg
    11401 cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc ccataaaggt
    11461 ggcaggatcg gcacccagcg ccgcaccaag acgcgacatt tcagccagcc cacgggtgat
    11521 cagcgccgta cgcgcattcg caccaaaacc gataccgtca gacatccccg cgccaatggc
    11581 aatgacgttt ttcaccgcgc cgccaagctg cacgccaatg aaatccgggt tgctgtaaac
    11641 gcggaaactt ttgccgcagt gcagcagctg ctggagatca tcggcaaagg tctgatcggt
    11701 cgaggccagc gaaatagctg tcggtaaacc tgccgccagt tctttcgcaa acgttgggcc
    11761 agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt cctgtaacag
    11821 acgtccggtt tccgcttcca gccctttggt cgcccacacc agacgcgcat caggacgcat
    11881 cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga cgacgagaat
    11941 attacgactg gctgccagcg cagtggcgag atcgctttca agatggagcg tatcgggaaa
    12001 aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg cgatatgttc
    12061 agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtaa tggcaagagc
    12121 ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt tcatcaggca
    12181 tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat gaacagcgca
    12241 tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac catgctggtg
    12301 atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag gcaatgtgcc
    12361 atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac ttcacacagg
    12421 aacgcggttt cttcgcccaa ggaggccgtt acggtaacac gcagtaccac ttcgtatacg
    12481 tcatctgcca gttgggaaga tgccgtatcc agatcaagtt taacttctgg ttgccaatct
    12541 ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga tatccttggt ataaatacgt
    12601 tggatctgga aagtcatttc agtgttgttt tgttctgaca tgtgtagaaa acccttaagt
    12661 gttgtcctta aatactgcgc aatgtaatac caacgttcac acgttatttc agcaggggat
    12721 ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg tgctgtgcgt
    12781 caataaaaat ctggggaacc gtggtgcaac cgctgcgttt gatcatctct tcacgcttgg
    12841 cggcattgcc atcgatcggc aactcctgga aactcacgcc cttgctgctc agcagtgctt
    12901 ttgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg gccataattt
    12961 agctcctgtt tttttaccct gaaatttcat gttgcagggc ggctgcaaaa tggagtgtcc
    13021 ccagaagctt ataacaataa gtcgctgggg gcctaattgc agctaacggc ctgcatcatg
    13081 aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa gttttcgcca gcccagccag
    13141 caacgccctc tttcagcacg aatacttgcg caaaaccagc tttcgtcagc gcgtttgcag
    13201 gctcctggca ctgcatgcca gaaccgtcta ccacgataac cgatttgtct ttgtgcttct
    13261 caagctcacc aacattgttg gctttgattt cgctcggcaa cagattgata gaacctgcga
    13321 tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg tcttctttgt
    13381 tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa
    13441 acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt atgggatgac
    13501 ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa
    13561 aaaaccagga aaggagtata cctgcgcggt gcggcaaata cagccagcgc gttaactgga
    13621 atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc tggcatcggc ttgccgcgag
    13681 cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc
    13741 aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac
    13801 tggtgattct ggatggctat ggctatcgtg aagaacagca ggataacgcc atttttagtg
    13861 ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt
    13921 ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta
    13981 acctgggtgc cggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaag
    14041 atcgcgcttt ctttgctaat ccagtgctgg ctggcgcggt acataaagcg aaaaacgcag
    14101 gtaaagcagt acacattatg ggtctgctct ccgcaggcgg cgtacacagc cacgaagatc
    14161 acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg
    14221 cattccttga cggtcgcgac accccgccgc gcagtgctga atcctcgctg aaaaaatttg
    14281 aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc catcattggt cgttactacg
    14341 cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ctatgacctg ctgactctgg
    14401 cgcagggtga gttccaggcc gatactgccg ttgctggttt gcaggcagct tatgctcgcg
    14461 acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg
    14521 caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc gcgcgtgaaa
    14581 tcactcgtgc attcgtgaac gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg
    14641 tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa aactgcggtt gcttacccac
    14701 ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc
    14761 gtatttccga aaccgaaaaa tatg
//
LOCUS       DAJTYL010000097        14702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYL010000097 DAJTYL010000000
VERSION     DAJTYL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.097"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..709)
                     /gene="pdeF"
                     /locus_tag="OO572_002969"
     CDS             complement(<1..709)
                     /gene="pdeF"
                     /locus_tag="OO572_002969"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6661893.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNE"
     gene            complement(891..1043)
                     /locus_tag="OO572_002970"
     CDS             complement(891..1043)
                     /locus_tag="OO572_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661894.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1061..1252
                     /locus_tag="OO572_002971"
     CDS             1061..1252
                     /locus_tag="OO572_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6661895.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            1563..2081
                     /locus_tag="OO572_002972"
     CDS             1563..2081
                     /locus_tag="OO572_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6661896.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSVVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            2097..2636
                     /locus_tag="OO572_002973"
     CDS             2097..2636
                     /locus_tag="OO572_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6661897.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(2731..4308)
                     /gene="guaA"
                     /locus_tag="OO572_002974"
     CDS             complement(2731..4308)
                     /gene="guaA"
                     /locus_tag="OO572_002974"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6661898.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(4377..5843)
                     /gene="guaB"
                     /locus_tag="OO572_002975"
     CDS             complement(4377..5843)
                     /gene="guaB"
                     /locus_tag="OO572_002975"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6661899.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            6005..7375
                     /gene="xseA"
                     /locus_tag="OO572_002976"
     CDS             6005..7375
                     /gene="xseA"
                     /locus_tag="OO572_002976"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6661900.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(7372..7587)
                     /locus_tag="OO572_002977"
     CDS             complement(7372..7587)
                     /locus_tag="OO572_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6661901.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(7657..9129)
                     /gene="der"
                     /locus_tag="OO572_002978"
     CDS             complement(7657..9129)
                     /gene="der"
                     /locus_tag="OO572_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6661902.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(9247..10425)
                     /gene="bamB"
                     /locus_tag="OO572_002979"
     CDS             complement(9247..10425)
                     /gene="bamB"
                     /locus_tag="OO572_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6661903.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(10436..11056)
                     /gene="yfgM"
                     /locus_tag="OO572_002980"
     CDS             complement(10436..11056)
                     /gene="yfgM"
                     /locus_tag="OO572_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6661904.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(11074..12348)
                     /gene="hisS"
                     /locus_tag="OO572_002981"
     CDS             complement(11074..12348)
                     /gene="hisS"
                     /locus_tag="OO572_002981"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6661905.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(12451..13578)
                     /gene="ispG"
                     /locus_tag="OO572_002982"
                     /gene_synonym="gcpE"
     CDS             complement(12451..13578)
                     /gene="ispG"
                     /locus_tag="OO572_002982"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6661906.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            complement(13605..14618)
                     /gene="rodZ"
                     /locus_tag="OO572_002983"
     CDS             complement(13605..14618)
                     /gene="rodZ"
                     /locus_tag="OO572_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6661907.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
BASE COUNT         3700 a         3973 c         3530 g         3499 t
ORIGIN      
        1 tttcatttaa cggcatgcat aacagcaaca ataaagcacc taatgccagt agccagagcg
       61 cgaactcttt tttggtgact ttggcatcaa cctgctgttt taattgcgaa taatagctac
      121 gtaaataaaa tggatttcgc actacccgaa tgatgaagta gcacagcggg acaccgatca
      181 gattacccac cagtaaggcc tgataattga ttaaagtccc gaggttaaaa ggcatgacac
      241 cgaccagatt ttctctgctc gccagtaatc ctacaaatgc agcaaactgg aaaagtatca
      301 gaaacagcgt tgcaggaaac actatctgcc agaatatacg ctgggaaatt aaacgggtat
      361 caccatgtga aacgttgtta cgccggggag taaagaccct gtaaccgccc caacagagaa
      421 tgataatgat aataaaatta gccgttaatg agagcgtttc gtaaaaaccc agctctgcat
      481 atttgcggac aaaaatccct aaggtgatcc ctggcaatgc cgcccagctg aaaaacatca
      541 tcatgctgat catcagtgcc aaaggcagat aaaagagaaa aacctcaccg gaagaaatat
      601 gcgcgaaagt attaatgtgg gcgaaaatgg gcaagattaa agaaggcagg aacagcggaa
      661 gcccccacca tttatcacgt atttttatat aagttgcatt cagtttcata gatgctcagc
      721 agaatccccc acatcctgaa ggaggtgtat tcagacaggc atcccacctg acttcgaatg
      781 atgattattc atcactatag agagcattga ttctaagtgt catatgaaag ttccaattga
      841 tatatatcaa acaaaataac cctgattaat gaattattac gtttatcatg ttaattcatc
      901 attattacat catcattgta aataattaaa ttaacttcca taacattaaa atatgtatcc
      961 actgacgctt ttttacataa cgaagaattg accattttgt cctgttgtgc cttaatgtaa
     1021 gtaccgtcca cagcgtggga catacttcaa ggaacctttt gtgagtcagg caactagtat
     1081 gcgaaaacga caccgattta acagtcgcat gacccgtatc gtactgctca tcagctttat
     1141 cttcttcttt ggccgtttta tctactcgtc cgtcggtgcc tggcagcacc atcagagcaa
     1201 aaaagaagct cagcaatcca cactctccgt cgaatcaccg gtacaacgtt agcggttacc
     1261 ttctccactt tcacagaaca taacggcact tcgctgtcgg atgcttttgc tctttgggat
     1321 tatcaaagcg gcagatattc tttcatctta aattttacgt ctttgtcctg actgatgttt
     1381 atcctgtttg gctgcgaaat aaatataaaa ttaatatata tgttgtaatg atatattttt
     1441 ataaattatt ccctgcgtga attttaataa atttaatcta tccctttata cgcaatacat
     1501 ttactttcct cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa
     1561 tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg
     1621 caggatgcca gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga
     1681 ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg
     1741 tagacaactc ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg
     1801 ctggcggtgt tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg
     1861 cagttggcgg cggagctgta ggcgcggcag cgggttctgt ggtgaatgat aaaaccttag
     1921 tggaaggtgt ttctttaaca tataaggaag gcaccaaagt gtatacctcc acccaggagg
     1981 gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa
     2041 cgcgtattca gccaaatacc aaatgtcctg aaaagagcta ataatcagga ggagtcatga
     2101 agaaagtttt tctttgcgcc atcttagcct ccttaagcta tccggctata gcctcatcat
     2161 tgcaggatca actctctgct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa
     2221 ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc
     2281 gcgcaaatgc ccaggccgcc gcgaacaaaa gagcggcaac ggcagcggca aataagaaag
     2341 ctcgtcagga taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg
     2401 aagatgagct acgcagctta gagattcaga aacaaaaact ggcgctggcg aaagaagaag
     2461 cccgcgttaa gcgcgaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa
     2521 ccgatgtggt gcaatctgaa gctgacgcaa acagaaatat gactgaaggc ggtcgcgatc
     2581 tgatgaaaag cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga
     2641 tgttagtaac ttcaagccta tgattcttga agataaaaaa ccctctgtag taacggaggg
     2701 ttttgttcat tcatagtgca gggatcaaaa tcattcccac tcaatggtag ctggcggctt
     2761 gccgctgatg tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga
     2821 aacgcgaccg aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt
     2881 ttcgacagca cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac
     2941 ggaacgtact ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca ggtccgcttt
     3001 acgcagttct tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt
     3061 cacttcaccc agaacacgaa cgccaaggcc tggtcccggg aacggatgac ggtacagcat
     3121 gtcgtacggc aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt
     3181 cagcggttca accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga
     3241 tttgatgacg tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat
     3301 ggtgccctgc gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc
     3361 aacgaaaacg cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc
     3421 tgacaggaag cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa
     3481 catatccaga acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca
     3541 agtcaggttt ttaccgatag cacggtgcag cagcattgcg gttacggagg aatccacacc
     3601 accagagagg ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc
     3661 atcgtcgata attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa
     3721 acgctccagc atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc
     3781 atagaagcgt ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac
     3841 ggtgacgaag tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag
     3901 cagcggttta ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt
     3961 tacgacttct acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc
     4021 caactgcatt gccatggtct gcatgccata gcaaacgccg aatatcggta cgcctgcttc
     4081 aaagacatac tgcggcgcac gcggactgtt ttcttcagta gtactttccg ggccgccgga
     4141 aagaataatt ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca
     4201 cagttcgcag taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga
     4261 accgaagtcc agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc
     4321 cgaggcaagt gaaacagata atataaatcg cccgacatga aatcgggcga agagaatcag
     4381 gagcccagac ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc
     4441 tgaatgcccg caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta
     4501 ccacagccgg tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct
     4561 ttcaggcgac ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca
     4621 gcgttatcgc tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc
     4681 ataccacggt aagatttata agaacggccc tggtagagtt cgatttcacc cggagattct
     4741 tcagtacccg ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg
     4801 gcgatgtcgc cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc
     4861 agggcttcaa ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata
     4921 cgagtcgtac agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct
     4981 gccaaagcgc gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga
     5041 tatttagcac gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag
     5101 tcgatcagca gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc
     5161 gcacctgcgc caactgcagc accaacacgc agacggcctt gctcgtcttt acaagcgttc
     5221 ggtttacgtt ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg
     5281 tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca
     5341 cgggcttcac cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc
     5401 ggctggttca ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg
     5461 ttttcttcgg taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact
     5521 tcgcgcagcg tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt
     5581 tttttcacac ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag
     5641 ccgataccgc cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt
     5701 gctgcggaaa gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca
     5761 gcagtattcg gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga
     5821 gcttctttag cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc
     5881 cgcggcatta tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct
     5941 atccccgaag ggcgggttac tatagactga ataacctgct gatttagaat ttgatctcgc
     6001 tcacatgtta ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt
     6061 tcgtctgctg cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt
     6121 cacacaaccg gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg
     6181 ctgcgcgatg ttccgcaaca gcaaccgccg ggtcaccttc cgtccgcagc atgggcaaca
     6241 agttttagtt cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt
     6301 tgagagtatg cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc
     6361 gaagttgcag gctgaatgtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc
     6421 gcattgcgtt ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt
     6481 gttaaaacgt cgcgatcctt ctctaccggt gatcatctac cccacctccg ttcagggcga
     6541 tgacgcgccg gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga
     6601 cgtgttgatc gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga
     6661 acgcgtagcg cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga
     6721 gacggatgtg accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc
     6781 cgctgaagta gtgagccgta atcagcaaga gttactgcgc caggtgcaat cggcccgtca
     6841 acggctggag atggcgatgg attattatct cgccaaccgt acacgtcgct ttacgcagat
     6901 tcatcatcgc ttgcagcagc agcatccgca gctccggctg gcacgccagc aaaccatgct
     6961 tgaacgcctg caaaaacgga tgagctttgc gctggaaaat cagcttaagc gtgccgggca
     7021 acagcagcag cgattaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg
     7081 cacgcaaacg cgcattcagc aactggaata tcgtttagca gaaatcctgc gcgcacagct
     7141 tagcgccacg cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact
     7201 gtcaacgctg gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa
     7261 agtgaagcaa gttaaaacgg gtgaaatgct aaccacacgt ctggaagacg gctggataga
     7321 aagtgaagta aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggttc attaagccag
     7381 cacgaattca acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac
     7441 tgcaccgcag gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat
     7501 ttcaataaat tccccgcagg atgggcaacg cgcatggccg ttatcctgat caagcacatg
     7561 ctgacatttt ggacaatgca gttccatatg aattcctccg gtagccattc cctctacatt
     7621 catagaggga atggcagata aaatacttac ggataattat ttatttttct tgatgtgctt
     7681 catcagacgc ttacgtttac gcatctgggt tggcgtcagg gtgttacgct tgttcgcata
     7741 cgggttttcc ccttctttga actgaatacg aatcggcgat cccattacgt ccagcgattt
     7801 gcggaagtag ttcatcaagt agcgcttgta ggaatcaggc aggtctttca cctgattacc
     7861 gtgaatcacc acaatcggcg ggttataacc accagcgtgg gcatatttca gcttcacacg
     7921 acgaccgcgt accagcggcg gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag
     7981 catagaggtc cccacacgac gggtggagct gtcatacgct tcacgtactg attcaaacaa
     8041 gttaccaaca ccactgccgt gcaaggcaga gataaagtgc acacgagcaa aatcgataaa
     8101 gcccagacgg aagtccagcg tttctttcac ctgctctttc acttcctggc tcaggccatc
     8161 ccacttattc accacaatga caagtgagcg cccactattg agaataaagc ccagcagcga
     8221 gagatcctga tcggaaatac cttcgcgcgc atcaatcact aacatcacca cgttggcgtc
     8281 ttcaatggcc tgcaacgttt tgattacgga gaatttctct acagcatcgg tgattttgcc
     8341 gcgtttacgt acgccagcgg tgtcaatgag cacatactca cgtccatcgc gttccattgg
     8401 gatgtagatg ctgtcacgcg tcgtgccagg catgtcgtaa acaacaacgc gctcttctcc
     8461 aagaatacgg ttagtgagtg tagacttacc tacgttcgga cgacccacaa tcgccagttt
     8521 gatcggcaga ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc
     8581 ttcaaattgc gcccagtatt cagcgtcttc gtcgacttcc tcttgcggtg cgagatcttc
     8641 catccacggc agcagcacat gctccagcag acttaatacg ccacgaccgt gagacgcggc
     8701 gatcgggtag atttcaccta aaccaagcga gtagaaatca accactgcct gatcgggatc
     8761 cagaccgtca gttttgttta ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt
     8821 ggcaatcgct tcatctgccg gcatcaggcc cgcgcgcgca tccaccataa acagtacgac
     8881 gtccgcttct tcaatcgcca gcagcgactg ttccgccatg cgggtttcta caccgtcttc
     8941 tgtgccatca atcccgccgg tatcaataca gataaactca cggccttcaa tttccgcacg
     9001 accgtactta cggtcacgag tcagacccgg gaaatccgca accagcgcat ctcgggtgcg
     9061 agttagacgg ttaaataacg tggattttcc tacgttaggg cgcccgacaa gcgcgaccac
     9121 aggtaccatg tttaaagcct catttttata aatcatcaga caacgcacgc tatattcgcg
     9181 tcgttgttaa aaacaggaaa acggcccctg tccaggagcc gttttcaaag tgaacgacag
     9241 agacgattaa cgtgtaatag agtacacggt tccgtctttt gcctggatca gcagtttgcc
     9301 gtcagcggca accggttcag tctggaaacc ggaactatca actttttgct gggcaacgaa
     9361 acgaccatct tcgacgttaa tccagtgcag ataaccttca ctgtcaccga ccaccaggtt
     9421 gccattatac agcaccggag aagtcagcag gcgatgcagc agatcgcttt gtgcccacag
     9481 cgtaacgccg ccatcaatgg tcaacgccat cacccggtca ttttgatcga ccagatagat
     9541 gcgattgcca tcgacgatga aatcattcac cgaacccagt tcgcgtttcc acataatctg
     9601 accactgcgc agatcaagcg ccgtcaggtt accattatag gccagcgcga aaacaacgcc
     9661 gttaacaacg acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt
     9721 cgcctgggaa atacgctgct gccaaatcat ctggccctgt tccatcagca ctgcgctgac
     9781 gcgaccatta tcgccaccca cgacggccgc accaaaagcc gttgccggcg cagactcgcc
     9841 acgcaaagag agcgaaggca tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc
     9901 gttcagcgct tgtaactgac cgttactggt gtggattaac accagaccgt cgctgaccac
     9961 cgggcgcgaa agtgcttcac ccgcgacttt agtttgccat gccacagtac catcgctggt
    10021 attcagcgcg taaacctgcg ccttttcgct gccaatgtag acatgcccac cagacacggt
    10081 cacaccgcca gaaagtaatg caggctcttt agagaaccag ccatctttct cggccaggct
    10141 gacagaccag atttctttgc catcatccgc attcagcgct tttaccaaac cagcgcggtc
    10201 cgctgcatag acaacgttgt ccgccagtgc cggatgaaga ttggaataga agttgccaat
    10261 gccgctacca acggaagtgc tccacgccgt ggtcggcgta aactggtttt caacggttgg
    10321 caatggggac atctttacca catcttcttc gctgttaaac agcgaacagc cgcttaaaag
    10381 ggtaacggaa agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga
    10441 tggacaaatt attaattttc atctgcatca tttcgctcag tgccggagta acatcgcttt
    10501 tcacgcctgc ttcccatgca ctacgcgcac cttgcttatc acctttgctc agcaatgctt
    10561 caccacgcaa gtcggcaaca atggcagccc acccttcacc tttgatggta tcaagggttt
    10621 tcagcgcggc atcagcctgc ttgagctgta cctgaacgcg agcaagacgc agatttatca
    10681 cggctttgag attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt
    10741 tctctagttc atttttgtca acaaattgct gcgcaagttc caaagaagcc agcgcaccat
    10801 aagtattttt attttcagca gcaaattttt ccgccgccgg gatgctatcc ggtttgcctt
    10861 cgctcactgc ggtaaccgca ttttgatagg caagagaagc ggagcgtgca gaatcaacct
    10921 gatggctgtt ccagtagcgc cagccagtca gtgcgccaac gcccaaaatc cccccaacag
    10981 ccagtgcttt gccattttca gcaaaaaagc gtttaaccgc ttctacctgg tcgttttcgt
    11041 tctcgtaaat ttccacgctg tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc
    11101 ggctacgcta tcctgcgcaa ctgccgtttg ctcaccagag cgcaaatcct tcactactgc
    11161 tgtgccgtta gccacttcag actcacccag caccacagca acgcgggcac cccatttatc
    11221 agcacgggca aactgtttct taaagttgcc gccgccgtgg ttggtcatca atttcacgcc
    11281 cagtaattca tcacgcagac gctcagctaa tgccatagcc gcagattgtg tatcagcacc
    11341 tgaagccacc aggtatatat cgacaacagg atcggcttta aattccggat taacggcctg
    11401 tactaacaat acaagacgtt cgaggcccat cgcaaaaccg acagccggtg ttgcacgacc
    11461 gcccagttgt tccacaagac cgtcataacg accgcctgca cacacggtgc cctgggagcc
    11521 gagactgtta gtcacccact cgaaaacggt acggttatag taatccagac cacgcaccag
    11581 acgctggttt acggtgtaag cgatccccgc gctctccagc agtttgcaca gaccggcaaa
    11641 atgctcacga gattcctcgt ccagataatc acctaatgcc ggagcgtcgt tgagaagcgc
    11701 ctgcacttcc ggattttttg aatccagcac gcgcagcggg ttagtgtaca tgcggcgttt
    11761 gcagtcttcg tccagctttt ctttatgctg ctcaaggaat gccaccagcg catcgcggta
    11821 attggcgcgt gcttccagcg aaccgataga attcagctca agagttacgt gttcgaagat
    11881 acccagcgca cgccaccagc gggcagtgag cataatcagt tcagcgtcga tatccggacc
    11941 ttgcagaccg aaaacttcgc agcccaactg atggaactga cgataacgcc ctttctgcgg
    12001 acgctcgtga cggaacatcg gcccgatata ccacagacgc tgttcctgat tgtacagaag
    12061 accatgctcg atgccggcgc gtacacagcc cgccgtccct tcagggcgca gagtcaggct
    12121 gtcgccattg cgatcctcaa aggtgtacat ctctttttca accacgtcgg tgacttcacc
    12181 aatcgcacgt ttgaatagcg gggtctgctc tacaatcggc aagcggattt cactgtaacc
    12241 gtagctgccg agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc
    12301 aggcaggtaa tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt ttattctctt
    12361 tctgaatata aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc
    12421 aatcatacac gggaagcgag gcgcttccca tcacgttatt atttttccaa cctgctgaac
    12481 gtcaattcga cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cttccagctg
    12541 gtcgatcata tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agaggccgct
    12601 tttcttgttg ccgccggtga cgccgagtgt agaaaccagc gcctcacctg ggccattcac
    12661 cacgcagccg ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag
    12721 cgcgttaacc gtaccgataa catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa
    12781 gttaatccct cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga ctttgatctc
    12841 ttcgaccgga tcggccgcca gcgatacacg cagcgtgtcg ccgatgcctt ccgacagcag
    12901 cagacctaaa ccaatggcgg attttactgc cccgctgcgt gcaccaccgg cttcggtgat
    12961 ccccagatgc agcggctgat cgatctgttt tgccagcaaa cgataagact caacagcgag
    13021 gaagacgtca gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg
    13081 atcaacatga cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc
    13141 ttgcagatct ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc
    13201 gcgcgcacag tcaaccacca tgcgaatacg ctcttcatta ccgatattac cagggttaat
    13261 acgcagacaa tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat
    13321 gtcagccacc agcggcacgt taacccgctg tttgatgagt ttgaacgctt ctgccgcgtc
    13381 catcgtcggt acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg
    13441 attgaccgtt gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg
    13501 agcaccatcg ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat
    13561 tggagcctgg ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat
    13621 tgttcggcat tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg
    13681 acaggtttcc cttgatactg gatctgtact gcggctggcg caccaatttt cagtttatac
    13741 ggtgcctggc cggttaaatt caaattaccg tctttacgct gcatcccgct aaacaatttt
    13801 ttaccggtag catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca
    13861 ttcggatcag ccgccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca
    13921 tctggcgttg ttgctgccgt tggtaccggg gtcgcggcgg tatcaacatt tgcctgcgaa
    13981 ggcgaaacaa ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt
    14041 gtttgcgtgt tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg
    14101 tctgtagttg tcgacgtatt taacggaaca ctctgcccct gctcgctatt actgctcagt
    14161 tccgccgaag attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc
    14221 caccaccagg caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatt
    14281 agccagccgt cgcgtttttt gcggcgttta ccgagggaaa aactctgcat tggcgcaact
    14341 tttgcagccc gaagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata
    14401 tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg
    14461 ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca
    14521 acggcctgct gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta
    14581 agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg
    14641 agaattgaga tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta
    14701 ac
//
LOCUS       DAJTYL010000098        14602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYL010000098 DAJTYL010000000
VERSION     DAJTYL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.098"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..925)
                     /gene="tdh"
                     /locus_tag="OO572_002984"
     CDS             complement(<1..925)
                     /gene="tdh"
                     /locus_tag="OO572_002984"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6661908.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDL"
     gene            complement(935..2131)
                     /gene="kbl"
                     /locus_tag="OO572_002985"
     CDS             complement(935..2131)
                     /gene="kbl"
                     /locus_tag="OO572_002985"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6661909.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2406..3263)
                     /gene="yibB"
                     /locus_tag="OO572_002986"
     CDS             complement(2406..3263)
                     /gene="yibB"
                     /locus_tag="OO572_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6661910.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3567..4499
                     /gene="rfaD"
                     /locus_tag="OO572_002987"
     CDS             3567..4499
                     /gene="rfaD"
                     /locus_tag="OO572_002987"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6661911.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4509..5555
                     /gene="rfaF"
                     /locus_tag="OO572_002988"
     CDS             4509..5555
                     /gene="rfaF"
                     /locus_tag="OO572_002988"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6661912.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5559..6551
                     /gene="rfaC"
                     /locus_tag="OO572_002989"
     CDS             5559..6551
                     /gene="rfaC"
                     /locus_tag="OO572_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6661913.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6548..7756
                     /gene="rfaL"
                     /locus_tag="OO572_002990"
     CDS             6548..7756
                     /gene="rfaL"
                     /locus_tag="OO572_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6661914.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7792..8934)
                     /locus_tag="OO572_002991"
     CDS             complement(7792..8934)
                     /locus_tag="OO572_002991"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6661915.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(8943..9956)
                     /locus_tag="OO572_002992"
     CDS             complement(8943..9956)
                     /locus_tag="OO572_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6661916.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(9981..10688)
                     /gene="rfaY"
                     /locus_tag="OO572_002993"
     CDS             complement(9981..10688)
                     /gene="rfaY"
                     /locus_tag="OO572_002993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6661917.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10714..11721)
                     /gene="waaO"
                     /locus_tag="OO572_002994"
                     /gene_synonym="rfaI"
     CDS             complement(10714..11721)
                     /gene="waaO"
                     /locus_tag="OO572_002994"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6661918.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11764..12561)
                     /gene="rfaP"
                     /locus_tag="OO572_002995"
     CDS             complement(11764..12561)
                     /gene="rfaP"
                     /locus_tag="OO572_002995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6661919.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12554..13678)
                     /locus_tag="OO572_002996"
     CDS             complement(12554..13678)
                     /locus_tag="OO572_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6661920.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13675..>14602)
                     /gene="rfaQ"
                     /locus_tag="OO572_002997"
     CDS             complement(13675..>14602)
                     /gene="rfaQ"
                     /locus_tag="OO572_002997"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6661921.1"
                     /translation="DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKY
                     DLIVNLTDQWMVAILVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLS
                     VLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKF
                     SAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDH
                     AQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQR
                     DRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL"
BASE COUNT         4131 a         3296 c         3004 g         4171 t
ORIGIN      
        1 agagatcgag gccagactga atcagcgccg ccatcttgta ccaggtttca aacatctcac
       61 gaccgtaaat gcctttaatg aacaagcctt taaagatcac tttggtccag tcgatagaca
      121 tatcagacgg tggaataccc agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca
      181 tggtacgaaa cgctggcggc gcgccggaca tttccagacc gacatcaaag ccttcggtca
      241 tgcccagttc agccatcaca tcattaaggt tttctttcgc gacgttaacc gcacgggtga
      301 tacccatttt gcgcgccagc tcaaggcggt attcgttaac atcggtgatc accacattgc
      361 gtgcaccaac gtgtttcgcc accgccgcag ccataatacc tatcggacct gcaccagaaa
      421 ccagcacatc ttcgcccacc agatcgaacg acagcgcggt atgcacggcg ttaccgaagg
      481 gatcaaaaat ggaagccagg tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg
      541 ccggaatcac cagatattcg gcgaagcagc ccggacggtt aacaccgaca ccaatggtgt
      601 tgcggcacag atgggtacga ccgccacgac agttgcgaca gtgaccgcag gtgatatggc
      661 cttcgccaga aacgcgatcg ccgattttga agcctttcac ttcctgacca atacctacca
      721 cttcaccgac atattcatgg cccacgacca tcggcaccgg gatggttttt tgcgaccact
      781 catcccagtt atagatgtga acgtcagtcc cgcagatggc tgttttacgg attttaatca
      841 gcagatcgtt atgcccgagt tccggtacag ggacgtcggt catccagatg ccctcttccg
      901 ctttcagttt ggataacgct ttcatctcac atcctcaggc gataacgccc agttgtttac
      961 caatacgcgt gaatgcttct actgcacgcg taatttgctc aggggtatgc gccgcagaca
     1021 tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt
     1081 aaatgccctc tttttgcagc tctcgggcaa atttctgcgc cactaccgca tcaccaagca
     1141 tgaccggaat aatggcgtga tcggctcccg ccagggtaaa gcccgccgcc gacatttgct
     1201 cacggaactg acgcgcgttc gcccacagac ggtcacgcaa ctcgctaccc gcttcaacca
     1261 tctccagcac tttgatggaa gcggcgacaa ttgctggtgc cagcgagttg gagaacaggt
     1321 acggacgaga acgctggcgc agccactcaa ccacttcttt gcgcgccgcg gtataaccac
     1381 cagaagcccc gcccagcgct ttaccgagcg tgccggtgat gatgtcgaca cgccccatca
     1441 catcgcagta ttcatgggaa ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt
     1501 cgtctaccat caccagggca tcgtatttat ctgccagatc gcaaacgccc ttcagattgg
     1561 caatcacgcc gtccatcgag aacacaccat cggtggcaat cagcacatga cgcgcaccgg
     1621 cttcacgcgc ttctttcaga cgagcttcaa gctcctgcat atcgttgttg gcatagcgat
     1681 agcgtttagc tttacacaga cgcacaccat caataataga cgcgtggttc agtgcgtcgg
     1741 agataatggc gtcttccgcg cccagcagcg tttcaaacag gccaccgtta gcatcaaagc
     1801 aggaagagta gagaatcgca tcttccatcc ccaggaatgc cgccagtttt tgttcaagct
     1861 ctttatggct gtcctgagtg ccgcaaataa aacgcaccga agccatgccg aaaccgtgag
     1921 aatccattcc cgcctttgct gccgcaatca gatccggatg attcgccagc ccgagatagt
     1981 tgttggcaca aaagttaatg acgtggcttc catcagccac ggtgatatct gcttgctgcg
     2041 cagacgtaat aatgcgctct tctttaaaca acccttccgc ccgtgcggtt tccagatcgt
     2101 tggttaactg ctgataaaat tctccacgca ttgcgattct ccagacaggg caaattccag
     2161 cacatattac ccaaacttat aggtcgcgac gagatgatgc gttaacactt ctgcaaaatt
     2221 caggataaat aacgtgtatc ttgatgtctc tcaacaccta cagatagtag aaaaactgta
     2281 cagttttggc tgagcataat gatgagtgat tttactgcat tgatataaaa agaaaaaatg
     2341 atgccaaagc atcatttttc ctatctcagt tggcatcaaa acgctaccca ctaatggagg
     2401 gaaacctatt ttcttgaaag gaaaattctc agcgcctgca tttttgcccc taaagtgtta
     2461 ctcctgaagc agcgaaacaa atcgaaccat tttcctctcc ccagatagtt taggttgaag
     2521 agatcggaac gtttgtaata gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta
     2581 ttgtttagtg ttattttttg actttccaga actaatttat agaattcttt ccatttatgc
     2641 tgtgaaccaa caattgcgcc cccaataata tatacatgat tgccaatcat gaaatcaaaa
     2701 gcctgttgtt gagatgtaac agtaagccct ttttttatag tgaaaagatg catcttattt
     2761 tcatcaaatg ggaaatccca aattttcaat cctcgagtta catttggctt acggcaatag
     2821 ccaaaatcta tccacgcaac aagaggcgtt ttcactaaac ccatgttgat agctttattt
     2881 acaaaatagg ccttaaggtt acataccaaa acatactctg gtgaccagta ctctgggttt
     2941 tttaattgtc gaggctcaag tctatttgta aatgactcat ctttttgaat tttttcgata
     3001 cgacttctga tatatctaaa tttctttttt atatcgataa cgataaccgt tgttggtttt
     3061 ccgttgcgaa tcgcttcaat ccttggtttc aaatcgggag aagtgaaaat aatcatctca
     3121 ttttcaagtg cggccagacg ctcaaagtaa ctaaaataaa catcaactga acgagctagt
     3181 ttttcacgga accctttatt agcagtccaa tcccctctac caatgtcaaa atatgcagtg
     3241 ataattgtgg tggatgattt cattactaat aagaccatta atataaaaaa agaattatat
     3301 cataaatatt aacagaatgt atactgtata ttatccattc aaatgattcc gttatttatc
     3361 atacatcgga atattgatac taaagcacta ttcacatgca aaaccaacat ccgccatgaa
     3421 ggactagcta aaacccaaac tagtttgttg caattagcat ccttgcacct ctatgtaaag
     3481 ggctgaaggg attcggatgt gatggtatga ttacagacat tcgtgtctga gattgtctct
     3541 gactccataa ttcgaaggtt acagttatga tcatcgttac cggcggcgcg ggctttatcg
     3601 gcagcaacat cgttaaggcc ctgaatgata aaggcatcac cgatattctg gtggtggaca
     3661 acctgaaaga cggcaccaag tttgtgaacc tggtggatct ggatatcgcg gactatatgg
     3721 ataaggaaga cttcctgatc cagattatgg ctggcgaaga gttcggtgat gtcgaagcga
     3781 ttttccacga aggcgcatgc tcttccacca ccgagtggga tggcaagtat atgatggata
     3841 acaactatca atactccaaa gagctgctgc actactgtct ggagcgcgaa atcccgttcc
     3901 tgtatgcctc ttccgcagcc acctacggcg gacgcacctc cgactttatt gaatcccgcg
     3961 agtacgaaaa accgttgaat gtctacggtt actcaaaatt cctgtttgat gaatatgttc
     4021 gtcaaatcct gccagaagcg aactcgcaga ttgttggctt ccgctatttc aacgtttatg
     4081 gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc tttccatctc aacacccagc
     4141 ttaataacgg tgaatcaccg aagctgttcg aaggtagcga gaacttcaaa cgtgacttcg
     4201 tctatgtggg cgacgtggca gatgtaaacc tgtggttcct ggaaaatggc gtttccggta
     4261 tcttcaacct cggcaccggt cgtgcagaat ccttccaggc agtagcagat gctacgctgg
     4321 cttatcacaa gaaaggccag atcgaataca ttccgttccc ggataagctg aaaggccgct
     4381 accaggcgtt cactcaggca gatctgacaa atctgcgcgc ggcgggttac gacaaaccgt
     4441 tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg gctgaatcgc gacgcataag
     4501 agctctgcat gaaaatactg gtgatcggcc cgtcttgggt tggcgacatg atgatgtcgc
     4561 aaagtctcta tcgcacgctc caggcgcgct atccccaggc gataatcgat gtgatggcac
     4621 cggcatggtg ccgtccatta ttatcgcgga tgccggaagt taacgaagct atccctatgc
     4681 ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa actgggtcat agcctgcgtg
     4741 aaaagcgcta cgaccgcgcc tacgtcttac caaactcctt caaatctgca ttagtgcctt
     4801 tcttcgcggg tattcctcat cgcaccggct ggcgcggcga gatgcgctac ggtttactca
     4861 acgatgtacg cgtgctcgat aaagaagcct ggccgctaat ggtggaacgc tatgtcgcgc
     4921 tggcctatga caaaggcatt atgcgtaccg cacaagatct gccgcagcca ttgttatggc
     4981 cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg taatcaattt tcgctttcat
     5041 cagaacgtcc gatgattggc ttttgcccgg gtgcggagtt tggtccggca aaacgctggc
     5101 cacactacca ctatgcggaa ctggcaaagc agctgattga tgaaggttat caggtggttc
     5161 tgtttggctc tgcgaaagat catgaagcgg gcaatgagat tcttgccgct ttgaataccg
     5221 agcagcaggc atggtgtcgg aacctggcgg gggaaacaca gcttgatcaa gcggttatct
     5281 tgattgcagc ctgtaaagcc attgtcacta acgattctgg cctgatgcac gttgcggcgg
     5341 cgctcaatcg tccgctggtt gccctgtatg gtccaagtag cccggacttc acaccgccgc
     5401 tatcccataa agcgcgcgtg atccgtctga ttaccggcta tcacaaagtg cgtaaaggtg
     5461 acgctgcgga gggttatcac cagagcttga tcgacattac tccccagcgc gtactggaag
     5521 aactcaacgc gctattgtta caagaggaag cctgacggat gcgggttttg atcgttaaaa
     5581 catcgtcgat gggcgatgtt ctccatacgt tgcccgcact cactgatgcc cagcaggcaa
     5641 tcccagggat taagtttgac tgggtggtgg aagaagggtt cgcacagatt ccttcctggc
     5701 acgctgccgt tgagcgagtt attcctgtgg caatacgtcg ctggcgtaaa gcctggttct
     5761 cggcccccat aaaagctgaa cgcaaagcgt ttcgtgaagc gctacaagca gagaactatg
     5821 acgcagttat cgacgctcag gggctggtaa aaagcgcggc actggtgaca cgtctggcgc
     5881 atggcgtaaa gcatggattg gactggcaaa ccgctcgcga acctttagcc agcctgtttt
     5941 acaattgtaa gcatcatatt gcaaaacagc agcacgccgt agaacgcacc cgcgaactgt
     6001 ttgccaaaag tttgggctat agcaaaccgc aaacccaggg cgattatgct atcgcacagc
     6061 attttctgac gaacctgcct acagatgctg gcgaatatgc cgtatttctt catgcgacga
     6121 cccgtgatga taaacactgg ccggaagaac actggcgaga attgattggt ttactggctg
     6181 attcaggaat acggattaaa cttccgtggg gcgcgccgca tgaggaagaa cgggcgaaac
     6241 gactggcgga aggatttgct tatgttgaag tattgccgaa gatgagtctg gaaggcgttg
     6301 cccgcgtgct ggccggggct aaatttgtag tgtcggtgga tacggggtta agccatttaa
     6361 cggcggcact ggatagaccc aatatcacgg tttatggacc aaccgatccg ggattaattg
     6421 gtgggtatgg gaagaatcaa gtagagtgtc gttctacaag tatgtctctt gcagatttgc
     6481 cagctcaaac cgtttttcaa aatttaaatc ttgaaataat aaccaataag ttgacatcgg
     6541 agataagatg acctcaacat tatttttctc tctcgagaaa aaaaactgga tagcgtactg
     6601 gaacagagct ctcgtattct tattcattac cacctatttt ttgggtggga ttacaaggta
     6661 taaacatctt attgttattc ttatgacaat aacgacaatc gtctatctct gcaaacggcc
     6721 aaaacactat ctctcactat ttaaaacatt tctttttggt agtgttgcca tattaactat
     6781 tgctgcattg ctgtcacttc ttcaatcccc tgatgcaggt gctagcatga aggaagtttt
     6841 caaagctatt attgagaata ctttactatg cacaatagca ataccggtca tattgagaga
     6901 cgagaaaaga gaagatgtcg aaaaaatcgt tttcttctca tttattagtg cgttgggctt
     6961 acgctgtttt tctgaattga ttacctatta taaggactat caacaaggga taatgccatt
     7021 cgcagattat agacaccgta gcatttctga ctcgatggtc tttttattcc ctgcattgtt
     7081 aaatctctgg cttatcaaat cagcaaaata ccgcatttct tttgtggttc taagcgttat
     7141 ttttattttt ctgatattag gaactttatc cagaggggcc tggctttccg tgttagtcat
     7201 tggattaata tggattctga tgtttaaaca atggaagtta ctattagtag gagtaatggt
     7261 ttccatcatt gcattgtcgg ttattttcac acataaggag atgaccgcaa agctaacgta
     7321 taaacttcaa caaactaata gttcttatcg ctatgcaaat ggtactcaag gcagcgcact
     7381 cgatctaata ttagaaaatc ctgttattgg ttatggttac ggtaacgttg catataaaga
     7441 tgtctataat aaacgtgtca ttgattatcc agaatggacc tttagacaat caatagggcc
     7501 acataatttt gcgctattca tctggtttgg cactggttta ttagggctgg taagtcttat
     7561 gatgctatac tgtgcaatat tgaaagagtg tataaaaaat ggcgtcaaga ataaatatcg
     7621 ctcaccatat aatgcatatt atataatctt actatctttt ataggttatt ttgttatccg
     7681 tggaaacgta gaacaaattg aaccaaattt attaggcgtt tacgccggct tattattagc
     7741 gatgaaaaac aagtaaaaaa taaaaaaggc tgcataatgc ggccttttta tttattcaaa
     7801 ccaattatga ataacctctt cgaatctttg agttacgcca tcccaactgt agtgatcaaa
     7861 cacaaagtcc tgaccttgct ttgcaactgc tgttaattca ggattcgcta acgtttttaa
     7921 aatatcgctg cttatggagt ctgcagtcat aggttctttt aaatggaatc ccgtagtatt
     7981 ttctttgaca aattccgtca tccccccacg cgtactcact aaaacaggct taccagcacc
     8041 catagcttca attgccacca tgcaaaatgg ttcctggaac tgggaaggaa tgacgactaa
     8101 atctgccagt gggtaataac aatacatctt ttcaggcggt atgcttccta acataatgca
     8161 acgatccttc aaacgttttg caatttccct gacattgcgt tgatatgcac ctttgtcact
     8221 tttactcatt tctgtataat caccaatcac aactaatttt aaatttttgt gagcagcggc
     8281 caatttttcg aatgattgca tcaacagcaa tatgcctttg tcagggacga tacgcccagc
     8341 ataaaaaata attttctctt caggagagat attaagttcg cttctagtga tcggctgaaa
     8401 attagattga taggtttcta aatcaattcc atttggtaca atctcaatat ctgcatttgc
     8461 taaataagat tggtaatact ttttcaagta taaactaggg actatcattt taacattttg
     8521 ctcaagcaat tttggttcaa atgcattatg catatgtata accactcttg cctgaggagc
     8581 tagcttacga atttgagtgt atagcttcat gctattatga actacaataa cactttcttt
     8641 cgttatagga aaatcatgtg caatattcaa tattctctgt gaatatggaa gaggatcaag
     8701 tcttgtccat ttttgaaata accgcttata tatgcgactg aatccaaccc tatgaatact
     8761 acaattatca ttaacaaagg tgtaatttga gtatcctgga tttttgatac aaacaatacg
     8821 atttggaaaa ttggtacgct gagcaacctg atacatccac gtttcaacag cagccgctcc
     8881 gcgaggagga attgaaaaaa taggagtaac cgtaaatatt attttatcaa ccatctcgta
     8941 tattaacctt tcataacatt atatttaatt gctgtaaaaa caccatcgag aaattttttc
     9001 tggtaaagca agtgtttata tttttctttg tgctcatgtt ttttaacagc ttttttataa
     9061 ggtatatttc tccatggtga tatattataa atattacgaa aataatctgc agaggcgtag
     9121 ttcgcccaat catgccatgg cttagttata ccagtataat gtatgaagac agtgtcatca
     9181 ttaataaacc gggtgtaata ttcactattt ttttcttcaa attctgactt tatagtataa
     9241 atacaattat attttctcgg taatatttta gcatgatgta gaaacattaa gtttaaaata
     9301 tcttgatccg ggtactttaa tttttttata attgactcat ctgataatag atcaaagaat
     9361 ttttccgtta gtctttgttt taaccattcc cgtaaattaa tatacattac accagagtta
     9421 aaatagctac cgttaaaatc ctcattacac aaacgctcag cgcttttact ttgcatagca
     9481 tctacatcaa ttaccactgc cccatattca tctttaaact ctaatgctat taactcgttc
     9541 aatgaaccct tacagacgat atcagcatca agatataata atgaatccaa tcgcgcagaa
     9601 aaataatcaa atgaaagcaa acgataataa atagatactg gccagatatt tgattgaggt
     9661 agcgccttca atggctcaga atcaatatga tataatttaa ttactgtttg atacttcgtt
     9721 gctaattgtg ataataattc aatatactct ttgtctaagt aatcagcgat tatgtgaaag
     9781 gtaaatttgt tttgtttatt cttttcaaga attgaagcaa ttgaaatcgc agccccaacc
     9841 tggtagttct catcaatacc ccatgagaca ttaaacgttt cccgttcatc aagtttaacg
     9901 gggcgcttgt ccagttcaat tatatcttta aattgagtaa gatgtttaaa atccaattta
     9961 attacccttc atcataatca ttatttgctc ttcaatccta gtttgacttt tacatccctg
    10021 attacttttt taattttctt tttgaatata agatatgtat agccaaagtc tttcaattca
    10081 ttttttatat tatagtgccg ttcaagatca atgcgatctt ttgcttttaa aacagcggtt
    10141 gtttttttac ctgaaaggtc aattagtctt aaaccttttt cggatactat aaaatttcct
    10201 ttatgaggat cgccagacac cattccatgc tgatgcaact cttttatcga ttctgataat
    10261 tgatccttca gatcttcaga aatttccaaa tactcattta acccgacacc ttcaatatac
    10321 tcaatgagca taatataata atgagcgaaa tttaatgttt tacgttcagc tagcaaaaaa
    10381 tagtcattaa ttgattggat gccttcacca cgtacccgat cggtttgata aattaaattt
    10441 tcataataat ctttttttat gcaagatttt aagaaacgtt cggtcatttt atgctttggc
    10501 gcataaactt taagtacaag aggtccacga gcagtgtcaa taagaatgac tttagtatca
    10561 tcaatagaac gaaaaacttt taatgtttta accctgcaag ttaaaaagtc atttagaact
    10621 tgctcataaa agggatcatt atcttttatg aaaactttca acccatttat cgttttatta
    10681 tatatcataa tgaattattt taaccttaaa tctttagaag catttttctt tataatactt
    10741 taaataatta aaaatgcctg ccatataatg cttttgttta aatttatgtt ttgcacaata
    10801 ccgatactga ttgctattta caggtttaag taaatcttct ttactccacg gagaagctgc
    10861 tttggcgatc aaaaaactac gtgaaaccgg ataatcagcc cactcatgcc agggttttgt
    10921 aggtcccaca tagtgtataa acacggtgtc atcattgact ggattatcaa ctttgtcttt
    10981 taattcatag ttaatactat atcgggtatt atatttctcc gaaataaact tcactttacc
    11041 attcaataat acattcagta catcttgatc aaggtgggtg attttactta cccaatctgg
    11101 gtcacgcaac atttcaatag cttttgacga aatgttattt agattccact catcaatatt
    11161 tatcagtaaa aaaccagcat tgaaatatcc agaagctaac tgtggtgtag ttaataccga
    11221 ggctcgattt tgccaccatt caatatctct ttcagctaca actgcggcaa tttcattagt
    11281 agaaaattga taatctaaga gttctttaat actacccttg caagcaatat ctgcatcaag
    11341 atatagtatt ttttcatgtt tatgatagaa ataatctgca attataaatc gaaaatatgt
    11401 tgcgtaagtc cagtttttcg tgcttggtaa tgacttcagc ttatcacaat tgataacata
    11461 aatattaatt cttgaattat attgttttgc taaatcagaa aaatataatt tgtctttatc
    11521 actgatataa tctgtgaaaa tatgaaattc acaagagatt tctctattgt ttaatagaat
    11581 agatgcgatg gctacaccac atccaaaaag aaaatttctg tcaataccaa atgctatatc
    11641 aagagtattt ttagatctta tattttgata atgaaaatta tactcaaaaa ggatgatgtc
    11701 actatcattg agttgagaca tatttattct gtatccaaaa ttttggcaaa aataagttac
    11761 gggttacaac gattttcgaa tcgttctttc cctgattttt gtggcttttg cttctgcttg
    11821 cgacagcagt ccttgttcct gcttgagaat gtctttaagc ggggcggcaa aatacacctt
    11881 cataaagcgc cagatatccc gctgagtcag gccaatattc attgaagaaa aataaagccc
    11941 tataagatct ttatcgcgcc aacgacctgg aacgcgcgtg cgaagctgcg cccggtgtag
    12001 gtcaattacc gaaattttta actcctcttc cttaccggaa aaaggcaagt gcagcaggaa
    12061 atgacagata taacagtcac gatggttaat gcccgcagca tgcatatcgc gcaccatcgt
    12121 cgcgacacgc ttaataagca tacgctttac gcgaacatct ggtgggttgg tcgcccagtc
    12181 agcacagtaa tcttccagac tgatggttgg tgtcagatct tcggtaataa taaacgaggt
    12241 tctggtcagc ggattcatgc ctttttcgcc gaatgccacc ccatacatag tatcaacgcc
    12301 gacatcccgc agtcgatgaa tcgcattcca ttcgcggtct gcgcctaata ctggcatccg
    12361 caatgagagt aaatttttga ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa
    12421 atagcttttt cccgccattt caaagcgcag agtacggcga gtttccagtt cacgaaatac
    12481 ctcaccctgc aaggttttaa cttcctcaaa aggatctttg ccgcgccata acgtggcaaa
    12541 cggctcttta agttcaacca tccagaccac ccgttatgat atccgccgct ttctctggca
    12601 gactgtataa atcttgtgta tcagcataat gtcgcgcatt ttccgcccaa gcctggcgca
    12661 atgaagattg cgttaacgct ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct
    12721 cagcaatagc ctcgccgcaa ttagcgtcga caatataatg cgcatagcca caaacggcag
    12781 ttgttagtac cggtaatcct gcagtaatcg cttccagcag cacaattccc gccgcttcct
    12841 ggtaggcagg atgcagtaat aaatccgccg ccgccattaa ttccgagaca tcgttgcgcc
    12901 ccgagaagaa gtgaacatta ctgcgcacgc cgcgtttttc tgccagtgcc tcaaattttc
    12961 gcggtttatc ctgcccaaca acatatagca atgtgttgtg gcgcagcgaa tccggtaacg
    13021 aagcaagtgc ttcaatggaa cgatcggcac ctttacgcgt gaagtctgaa ccgacctgca
    13081 acaataaata ttgttgttcg gttattccat tcttcttacg gaagatttca cggctattgg
    13141 ctggctgctg gctatattta cgatcaggat aaatccccgg tggcagaata tgaaaacgct
    13201 ccgcttcagt ctgataatgt ttctggaaat cggcgatttg cttatctgtc agcatcagca
    13261 gctgtgtcgg cttgccctgt tcgaaggttg cccgctcaaa ggcggcataa tggcgataac
    13321 gtgacgtcag gcgatagaaa aagccttttt cctgcgctac tttctcggca taacaaacat
    13381 cagcggcata ataaacgtcc agccccggca ttttgttgaa tccaacgact ttatcgacgg
    13441 gatgttcgcg taaatgtttt tgcacccagg caaaatactc cgcattgcgc ccgtgattgg
    13501 tatgcgattt aaccggcact ttgatcactt caaatacatc agggcattcg ccttcccacg
    13561 actgggtata aacccgaaca tgatgacctc gggcggcgac tgtctgagca atacgcataa
    13621 aatcgcgctg caaaccgcca aagggaaaat atttatataa acaaaaagca acgatcataa
    13681 cgacgtacct gtcgtggagg agggcagtaa tttatcgaca gcagcaatga catctgccgc
    13741 cggaataacc gaaagataca tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg
    13801 gtaatctccc gcccagaatt gaatcatgtt atttgaccag ggacgccaga aaatatgatc
    13861 tgttgcgcca aacagcgata tcagcggcgt attaactgca gcggcaatat gcgccggtgc
    13921 ggaatcaacg ccaataaaca gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt
    13981 cacctttcca gccagcgccg ttactggtgg cgtctggcat ccctgcgcaa tttcattgac
    14041 gcaggccaga tcgtctttat ctgggccgga cgtcagaacg acttcataac cacgagcatg
    14101 taaggcatca atcacagcgg aaaacttggc gttgtcccag catttgaaga tttgccgcgc
    14161 cgtaggttgg ataaccacat aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat
    14221 acgtttccag cttgcaggcg ggtaactcat ggttgtctgc ttcaccaacg aatcaagtcc
    14281 caatggtgtc agcacggata agttactttc caccacattt ccaccctgca acggcaccaa
    14341 atgggtgaaa cttttacgcc aaaaagcaga ctgccgatga tgataatcct gggaaatttt
    14401 cacacgagca tttaataagc gaaccagtat agcaaccatc cattgatcgg taagattgac
    14461 gataaggtca tacttattgg cacgtaatac cttgatgaga tgaaaaaagt tggcaatttt
    14521 ttctgaggct tttgcttttt tattttttat gccgtagagc gcgttaatct ctggattttc
    14581 agacaggatc gggatggtgt cc
//
LOCUS       DAJTYL010000099        14010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYL010000099 DAJTYL010000000
VERSION     DAJTYL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.099"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(266..1804)
                     /gene="aldB"
                     /locus_tag="OO572_002998"
     CDS             complement(266..1804)
                     /gene="aldB"
                     /locus_tag="OO572_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6661922.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(1969..3120)
                     /gene="yiaY"
                     /locus_tag="OO572_002999"
     CDS             complement(1969..3120)
                     /gene="yiaY"
                     /locus_tag="OO572_002999"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6661923.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3310..5154)
                     /gene="selB"
                     /locus_tag="OO572_003000"
     CDS             complement(3310..5154)
                     /gene="selB"
                     /locus_tag="OO572_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6661924.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5151..6542)
                     /gene="selA"
                     /locus_tag="OO572_003001"
     CDS             complement(5151..6542)
                     /gene="selA"
                     /locus_tag="OO572_003001"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6661925.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6640..7248)
                     /locus_tag="OO572_003002"
     CDS             complement(6640..7248)
                     /locus_tag="OO572_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6661926.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7319..8404)
                     /locus_tag="OO572_003003"
     CDS             complement(7319..8404)
                     /locus_tag="OO572_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6661927.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8407..8769)
                     /locus_tag="OO572_003004"
     CDS             complement(8407..8769)
                     /locus_tag="OO572_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6661928.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9306..11219
                     /gene="mtlA"
                     /locus_tag="OO572_003005"
     CDS             9306..11219
                     /gene="mtlA"
                     /locus_tag="OO572_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6661929.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11314..12462
                     /gene="mtlD"
                     /locus_tag="OO572_003006"
     CDS             11314..12462
                     /gene="mtlD"
                     /locus_tag="OO572_003006"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6661930.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12462..13049
                     /gene="mtlR"
                     /locus_tag="OO572_003007"
     CDS             12462..13049
                     /gene="mtlR"
                     /locus_tag="OO572_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6661931.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13061..13270)
                     /locus_tag="OO572_003008"
     CDS             complement(13061..13270)
                     /locus_tag="OO572_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661932.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13554..13916
                     /locus_tag="OO572_003009"
     CDS             13554..13916
                     /locus_tag="OO572_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6661933.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3376 a         3770 c         3555 g         3309 t
ORIGIN      
        1 tgatttacgg cctggttcgc ccgctcacca tcgaaacgcg agattaaact cctgacaaaa
       61 cacgccccag aaacgcactt ctgttggcgt agtctgggtt attgcgcacg taggtttaaa
      121 acgtaaattg taacaacgtg cgctttgttt atgccggatg cggcgtgtac gccttatccg
      181 gcctacggtc aggttcccgt aggcatgata agacgcgtaa gcgtcgcatc aggcaatgaa
      241 tacccaatgc gaccagcttc ttatatcaga acagccccaa cggtttatcc gagtagctca
      301 ccagcaggca cttggtttgc tggtaatgct ccagcatcat cttgtgggtt tcgcgaccga
      361 tacctgattg tttgtagcca ccaaacgcag catgtgccgg gtaagcgtga taacagttgg
      421 tccacacgcg cccagcctgt atgccgcgcc ccatcttata ggccagatta ccgttgcggc
      481 tccagacgcc cgcgcccagg ccatattgcg tatcgttcgc cagctccagc gcttcttcca
      541 tcgttttgaa ggtggtcacc gccagcaccg ggccaaaaat ctcctcctgg aacacccgca
      601 tattgttctg accaaacaga atcgtcggtt cgaggtagta gccgtctttc agttcacctt
      661 ccagcagctt gcgccgcccg cctgtgagca cgtcagcgcc ctctttttta ccgatatcaa
      721 tgtagttgag gatggtttcc agttgcccgt gagaaacctg cgcgcccatt tgcgtcacgc
      781 tgtcgagcgg gttaccgctg cgaatgcttt cgacacggcg gatggcgcgt tccataaagc
      841 gttcgtagat tgactcctgc actaaggcac gactcggaca ggtgcaaact tcgccctggt
      901 taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa aaaggcatct tcttcatcca
      961 tcacatcagc aaagaagata tttggcgact taccgcccaa ctccagcgtc accggaataa
     1021 tgttttgcgt tgcgtattgc ataatttgtt ggcccacttc cgttgagccg gtaaacgcca
     1081 ctttggcgat gcgtttcgag gtcgccagat attcgccaat ttccccacct gcgccattga
     1141 ccacgttcac cacgcccggc ggcagtaaat caccgacaat ttccattagc agcagtacag
     1201 aaagcggggt aagacgtgcg ggtttcagca ccacacagtt gcccgccgcc agcgcgggag
     1261 ccattttcca gctcgccatc agcagcggga agttccacgg gataatctgc cccaccacgc
     1321 ctaacggttc atggaaatga taggccacgg tttcgctatc aacttcactg atcccacctt
     1381 cctgcgcccg aatacacgag gcgaaatagc ggaaatggtc aatcgccagc ggtacatccg
     1441 cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt ttcagctgtc gctaacagct
     1501 cgaggttttg ttccattcga tcggcaatct taaacagaat cgccgcgcga tcctgcaccg
     1561 aggtgtgcgc ccatttatct ttcactttgt gcgcagcatc cagcgccaga tcgatgtctc
     1621 gtttgcccga agacgccact tcgcacagca gctgcccggt caccggcgtc agattctggt
     1681 aatactcacc atcggcaggg gctacccatt caccgccaat aaagttgtcg tagcgggctt
     1741 ttaacttgat ggggaaacca tactcgccgg gcttaatctg tgctgaaggg ggattattgg
     1801 tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta gacgtgactg gcgaaatctt
     1861 cgccagtcgg taacagcttt acgacagcta tcacgaattt acgggcaagg attacagaga
     1921 tagttgaaaa agcggctgac aatttgccag ccgttgttgg aatgatgatt acattgcggc
     1981 gcgataaatc accacaattt cttcgtgagt tgcctggatc gggttagtaa agccacaggc
     2041 atctttcagg gcattagtcg ccagaaccgc gaaatcttct tctttcacgt tcaggtcgcg
     2101 taggcctgcc gggatatcca ctttcttcgc cagttcacgg atggcgttaa tgcaggcttc
     2161 agcaccttcc gcgtcgtttt tacctgtcac gttcacgccc attgcagcgg cacagtcacg
     2221 cagacgtgca gcggcgactt tgctgttgaa tacctgaaca tgcggcagca gaacggcgtt
     2281 acatacaccg tgtggcaggt tgtagaaacc gcccagctgg tgcgccatcg catgaacata
     2341 acccagagaa gcattattga acgccatacc ggcaaggaac tgggcataag ccattgcttc
     2401 acgcgctttc gcattactgc catcttcaac ggctaacggc aggttttcgg caatcatggt
     2461 cacggctttc agtgcacaag cgtcagtgat cggcgtggcg gcaatagaaa catacgcttc
     2521 gatagcgtgc gttaaggcat ccatacccgt tgcggcggtc agtgacttcg gcataccaat
     2581 catcagagag gagtcattga cagaaagcag cggagtcacg tgcttatcaa caatcgccat
     2641 tttgttatga cgcgcttcgt cagtgatgat gcagaaacgg gtcatttcag aggccgtacc
     2701 cgccgtggta ttgatggcga tcatcggcag ctgcggtttt gcagagcggt caacgccttc
     2761 gtaatcgcga atatcgccgc cattggctgc caccagcgca ataccttttg cgcagtcgtg
     2821 tggagaacca ccgcctaagg agatcacgct atcgcaatta ttttctttaa gtaatttcaa
     2881 acctgcggcg acgttttccg tggtggggtt aggttgggtg ccatcataaa taacgctaaa
     2941 aatattgcgt tcttccagtg ctttttgcac atcacccgcc atacctaatt tcgttaacat
     3001 attgtcagtg acaattaagg tacgggtaaa tccataatct gccatcatat tcattgcatc
     3061 agtcaatgaa tcagcgccga tgacattcac agaaggaata aagaaagttg aagatgccat
     3121 aacacactcc tgaaagtgta aaaaagggtg ggcagcttac tgaggatttt cattcgaaaa
     3181 catgatcatt attattaatt atcaaaatta atctaataaa aagcacattg tattcattaa
     3241 acaatgtgct ttatttaaaa tcataagaaa tttgtattaa tttattttaa aaattaatta
     3301 atcatttcct tatttttccg gaaataataa tgcgtcgcgc aataaatgat catttccacg
     3361 acgacgcgta aagccaatgc ggtcaaaata ttccagaatc tgaattgcca gctttcggcc
     3421 tacgcctaag cgatcgcgga aatccgccgc gcaggttgaa ccacactcct gatcgagatc
     3481 gcggatcata ttggcaaatt cgacaatccg atcgttacgg taataacgat ctttgacgat
     3541 cgcggtaatt attccttgct gcgccgcctg gcgtagcgtc aggcgcattg cctgctcgtc
     3601 ggtacccgtc tcttttgcca gatcacgcac ccaccacggc tcgtcaccaa acagcggctc
     3661 tgctttttgc cagatggcct gctgctcttc gctgaagccc gctttgtgat ccggcagatg
     3721 cagccaaccg tgatgactga ggatgtcgcc gctttcgcgc atcttttcaa tcagcaacag
     3781 tactagcgct tcatcttcca ttggcaacgc catacgtcgc agacgttcgc gcccaggccc
     3841 aggttcatcg cgatgttgct catgataagt cgctaatgtg tcgagaattt tccgttgcca
     3901 gcgagcggca accggcgcat tcaacaagct ataaccagcc tgaatatagc caggctgttg
     3961 cagcagttcg cgcatccctt cgccgttgag ctggcgcgcc caagcgaaat caggaagatt
     4021 aaccgcgccg cgttgcaggt gaacggctaa cgcttcgcta tcattctgtg ccctggcgag
     4081 ggaagccagc cattgcagat actctggttt acgtttaccg cgacgcggcg ggttaagcat
     4141 cacgacgcgc gctccggcca gcgtgttgcg ggcagagata tcgcgcaata ccaggcggtc
     4201 gttatctgcc agccataacg gggtgtcgaa gaccagttca gcaaggttat cttccagcag
     4261 tgaaacgcgt cccgtgacgt ggctggcggc gtggtgaata tgcagcggct gccactgggt
     4321 cagcggtgca tgggtttgaa gctcgacaat cacccgtgtg aacggctctg gcggcacatc
     4381 ggcaagcagc cagtcgccac ggttaatctg ctctttttcc gcatcacccg cgatgtcaag
     4441 cgcgatacgc tgcccggcat tggcggtttc tgttggctgg ttttgcgcat gcagcgcacg
     4501 tacacgcatc ggtttattta caccagtcag ccagagtgaa tcgcctacct tcacttcccc
     4561 gcttaacgcc gtaccggtga cgaccagccc ggcacctttt acggtaaatg cgcggtcaat
     4621 cgcgaggcgg aaactatgtt ggctggcgtg ctcgcgttcc ggcaactgaa gcagatgctc
     4681 gcgcagggca tcaattcccc gaccttcggt tgctgcggtg ataaacagtt ttgcctcagc
     4741 aaaaccgtat tcccgcagaa cctcctttac ctggcgttca acctcatcaa cacgcgcttc
     4801 gtccacgcga tcggctttgg tcagcgccac tgtcagcatc gggttaccgg tcagctgcaa
     4861 aatcgccaga tgctcacggg tctgtgccat cacgccgtca tcgcacgcca ccaccaacag
     4921 cgcgtgatcg ataccaccaa cgcccgccag catgttggaa agaaactttt catgaccggg
     4981 aacgtcgata aaaccaggca cgcgaccatc cggctgcggc cagtaggcat agccgagatc
     5041 gatggtcatg ccgcgctttt tttcttccgg cagacggtca gcatttacgc cagtaatcgc
     5101 ctgcaataag gttgttttgc cgtggtcaac gtgtccggca gtcgcaataa tcatttcaac
     5161 aacatctcca aaaaccgttg ctcatcttca aggcagcgta aatccagcca caatcgtccg
     5221 tcataaatac gaccaatcac cggcactggc aattcacgcc agcgggcggc taatgactca
     5281 aggtggctac cgcgtccatc atggggtgta aacgttaatg ccgcgctcgg caggcgatca
     5341 accggcagcg aaccactgcc aatctgcgaa agacatggca taacctgtac cgcaaactcc
     5401 gcgccgtaat gtgcgacaag gggagcctgt aaacgttgtg cctggatttg aatgacctct
     5461 gcgctgcggg taagcaggcg cagggtcggt aatttttcac tcagagcttc agggtgtaaa
     5521 taaagacgca acgtggcttc cagcgccgcg agggtcattt tatccgcgcg taatgcacgc
     5581 ttcagcgggt ggctttgcag gcgggcgatc atctcttttt taccaacgat aatcccggcc
     5641 tgcggcccgc ctaacaactt gtcgccggag aagctcacca gactgacgcc cgccgcaatc
     5701 aactcctgcg gcattggctc tttcggcaaa ccgtactggc taagatcgac cagcgagcca
     5761 ctgcctaaat cagtcaccac tggaacatcc agctctttgc cgagcgccac cagttcagct
     5821 tcgtctatcg ctttggtgaa cccctgaatg ctgtagttac tggtatgtac tttcatcaac
     5881 agtgcggtat tttcattcac cgcctgacga taatcattcg cgtgcgtgcg gttggtggtc
     5941 cctacttcgt gtagggtgca gcctgcctga cgcataacat cgggaatacg aaacgcgccg
     6001 ccaatctcca ccagttcgcc gcgagatacc accacctctt tcccgctggc agtggccgcc
     6061 aacatcaata acaccgccgc cgcattgtta ttgacgatac aggcatcttc cgcccccgta
     6121 atacggcaca gcagctgcgc cagcgcccga tcgcgatgtc cgcgtccggc gtcgtccaga
     6181 tcgtactcga gggtcactgg cgaacgcata gcctgcgcaa cggcttccac cgcggcttcc
     6241 gcctgtaaag ctcgcccaag gttggtatgc agcacggttc ccgtcaggtt gatcaccgga
     6301 cgcagcgcgc tctgcgcctc tttcgtcaac cgggcatcga cttcttgcgc ccagttttca
     6361 caccacgcag gcagcgtctg gctgccacga atcacttctc gcgcttcgtc gagcatctga
     6421 cgcaacaatt ccaccacgcg ggtgtgacca taagtatcac gcaaagaaag gaaggagcta
     6481 tcgcgcaata agcgatcaat agccggaagt tgactataga gggaacgcgt ttcggttgtc
     6541 ataggaaacc tggctgatca aggccctctc acacggagaa gggcgtttaa cataaccacg
     6601 gattgtaacg tgagatgggt caggaggaca tatcgcgcat caagcctttg gcggttcggt
     6661 gcgagcaaaa ctttcgcggc taaacaggtt ttcgaccagt ttgactaagt gtgggcggtc
     6721 gacacaccaa cctggcgcaa cgcggcggaa attgagatat ccgacagcac aggcaatggc
     6781 gatagtcgcc agattgaccg tatcggtttt gagtgtgcca tcgaccagat atccttccag
     6841 cacatccaga ctgcggttga ttttctcccg ctggcgtaac aattcatctt cagactgctg
     6901 cgccgccgga cgcgcctgtt cacgcaccga taccagcccg gcatccataa tgccatccgc
     6961 cagcgcctca attttgcgca cccgcaacga ctccagcgga tcgcgcggca acatcgccgg
     7021 agcgacattc attaactcaa tatattcagc gatgatcggc gaatcaaacc agcattcgcc
     7081 ctcttcggtc accagcaccg gcactttccc taacgggtta aattgcgcca cgccgttatc
     7141 cgcgttatag ggcagttcat taataaattc gaaagttatg cccttttcta acaacagaat
     7201 aaaaagtttg cgtacaaacg ggctggtgta gctaccgacg agtttcatgc cgagtccttt
     7261 gtgcgaggaa aaatatcagt atggctcagg ctcacgcaaa acacaggtga tataagtctc
     7321 aatgatccaa ataaagataa tgcatccaac tggtcattct tagcagcact ttccgcatca
     7381 ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg ggcgtagatg ccgtcgggta
     7441 aggcatcgat atcatcgtta gggtccagtt caatggttcc cagcacaccg tccgtgccgg
     7501 gaacgaccgt taatgattgc aataccccct gcgcctgata agaaccgcct ggcacgacag
     7561 gtaaaatact agtcaatttg ccgtggaaca cctgcccagg tagcgcgtta aacaccactt
     7621 ccgcatcatc gccaggtttc agacgtaaca gcgagttttg ccgaaattgg gcgacaattt
     7681 gccgtttttg ctcggggatg aagaccatca ccggacgcag cggcaaggca gctgcgtatg
     7741 tacctgggcg gatcagtacc tgagtgacgt agccattgct tggtgcgcga atgacagtct
     7801 gctcaagatt atattttgct tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct
     7861 cgccgttgac catactgtcg agctgactct ggatctgcgc ctgctccgcc accgagcctt
     7921 tcactaacgc atcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga
     7981 acggattcac cgccgcctgg ctgcctttca ggtaacgttg ataattttta aacagacggt
     8041 cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc gcacgcagcg
     8101 tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt
     8161 aacgaaccgg gtcgagctta aaaagcacct cgcccttttg aataagctga ttattcttgt
     8221 cagtgacttc agtaacaatt cccgtcacct gtggcgtgat agggatcgct atcactgcct
     8281 tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa caaaatcaaa ccactcacca
     8341 gaaacacgcc tcccagcgcc gccgtcgcca gcgccacgta agttaaaaca atcaatagat
     8401 ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg cgagttgatg
     8461 ctccagccgg gaaattcggt cagaaagtgc ggcgatttcc ggttcagtcg ctttctcctg
     8521 cgacgcaaca tgtgactgca taccccagcc acgctccggt tgatagagcg tcgcccagat
     8581 ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg ccgtatgaat
     8641 agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt catgtatcgc
     8701 gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg cgaaatagtt
     8761 tagaaacata tctgcctcgg attcacgttt atcagtgttg ttttttggct ggcagccaga
     8821 agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc ataacaccaa
     8881 agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg acgaaggcat
     8941 aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac
     9001 aaagattcaa caaaccatca aaacaaaaat gtgacattac tcacatttaa atgccatttt
     9061 tagcgaaaat caccgccttg tttctttttt atacaggcgt tttgtgatga gcgtcacgtc
     9121 aattacctct ctacccccta tatttatgtg actgatatca cacaaaagcc cgtcgactgg
     9181 acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct acctctgccg
     9241 ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata agaaggggtg
     9301 tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca
     9361 tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg ttatttattc
     9421 caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg atcacttatc
     9481 tcctaccgct gctgatcggt tataccggtg gtaagctggt aggcggcaaa cgtggtggcg
     9541 tagtcggtgc cattaccacc atgggcgtta tcgtcggcgc agacatgccg atgttcctcg
     9601 gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac cgctgggtag
     9661 acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca ggcatcatcg
     9721 ggatgatcct cgctattctg gcattcctcg gcattggccc gattgttgaa gccctgtcca
     9781 aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg ctggcgtcta
     9841 tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac ggtatcttct
     9901 cgccgctggg tattcagcag tcccatgaac tgggtaaatc aatcttcttc ctgattgaag
     9961 ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg
    10021 ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggtggtatc cacgaaatct
    10081 acttcccgta tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc ggcggtatga
    10141 ctggcgtgtt cacgctgact atcctgggcg gtggtctggt ttctccggca tctccgggtt
    10201 ctatccttgc tgtactggcg atgacaccaa aaggtgctta tttcgctaac atcgcgggtg
    10261 tgtgtgcggc gatggctgtc tccttcgttg tctctgctat tttgctgaaa accagcaaag
    10321 tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt
    10381 ctaaaggcac atctccgcta tctgcgggcg atgtgactaa cgacctgagc cacgtacgta
    10441 aaatcatcgt tgcctgtgac gccggtatgg gttcaagtgc gatgggcgca ggcgtgctgc
    10501 gtaagaaaat tcaggatgca ggtctgtcgc agatttcggt cactaacagc gcgatcaaca
    10561 acctgccgcc agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa cgcgctatgc
    10621 gccaggttcc gcaggcacag catatttcgc tgaccaactt cctcgacagc ggcctgtaca
    10681 ccagcctaac cgaacgtctc gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga
    10741 aagacagtct gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg
    10801 agaacatctt cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt tttgctggcg
    10861 agcagctggt gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg ctggatcgtg
    10921 aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt acggttgaag
    10981 cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct
    11041 tcggtgaaga agaagatgac attgcccgtc tggtgattgg tattgcagcc cgtaacaacg
    11101 agcacattca ggttatcacc agcctgacca atgcactgga tgatgagtcc gtcatcgagc
    11161 gtctggcaca caccaccagc gtggatgaag tgctggaact gctggcaggt cgtaagtaat
    11221 ccaatcctac cctctcctca cggagagggt ttgggtgagg gaaaagcctc accccagccc
    11281 tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt tggcgcaggt
    11341 aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca actgacgttt
    11401 gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta tcaggttcat
    11461 gtggtcggtg aaaccgagca ggtagatacc gtttccggcg tcaatgctgt cagcagcatt
    11521 ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc cgttggcccg
    11581 gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg caaagaacaa
    11641 ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg caccacgcag
    11701 ctgaaaggtc atgtgatgaa cgccctgccg gaagacgcca aagcgtgggt agaagagcac
    11761 gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc ggcaactaac
    11821 gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa aacgcagttc
    11881 aaaggcgcac tgccgaacat cccaggcatg gagttaaccg acaacctgat ggcatttgtc
    11941 gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct cggaaaactg
    12001 gccggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc ggtggtaaaa
    12061 ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga cgcagacaaa
    12121 catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct gaaagatgat
    12181 gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg tctgatcaag
    12241 ccactgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca ggggattgct
    12301 ggtgcaatgc acttccgcag tgaagatgat ccacaggctc aggaactggc agcactgatc
    12361 gctgacaaag gtccgcaggc ggcgctggca cagatttccg gtcttgatgc caacagcgag
    12421 gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac caggcgcagg
    12481 acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa caaacccagg
    12541 ccgttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga agctttctga
    12601 tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag gtattccgca
    12661 aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac ggtccgctgg
    12721 gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac cgccaggaat
    12781 acgaagatgc ggaactgctg atggcattgc gtgaagagct aaatcacgac ggcaacgagt
    12841 acgcctttac cgacgacgaa atccttggac cctttggtga actgcactgc gtggcggcgt
    12901 taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg caaattcagc
    12961 gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg atttccaaaa
    13021 tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc tctacctgct
    13081 tcggccgata aagccgacga taatactcca gacgttgtag atataacggc acgctttcat
    13141 cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac tcccggaacg
    13201 cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta
    13261 atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt
    13321 agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc aataatcatc
    13381 cgctgctgtt tattatcttt ttctatagat ttcttaatta atcaacgaat tgcgtagttt
    13441 ttgagcatgt taatgattac gttgcagaac tattacaatg tcatcagttg tgaacaaagc
    13501 acctggtcgc gcatactagg ggctataaat ttatctttat cagaagccat cacatgaaag
    13561 aagtcgaaaa aaacgaaatc aagcgtctca gcgatcgcct ggacgccatc cgccaccagc
    13621 aggccgatct gtcgctggtt gaagccgcag acaaatatgc cgagctggaa aaagagaaag
    13681 ccacgctgga agcagaaatt gctcgcctgc gtgaggttca tagccaaaaa ctgagtaaag
    13741 aagcacagaa gctgatgaag atgccgttcc agcgcgcaat taccaaaaaa gagcaggctg
    13801 atatgggcaa gctgaagaaa agtgttcgcg gactggtggt tgtgcaccca atgaccgcgc
    13861 tggggcgcga aatgggcctg gaagagatga ctggattttc taagactacg ttttaagccc
    13921 ctcagacgac tggttcgatc gtatcaaccc gcatctggct tgccttagct ggtcaatgcg
    13981 gggactctcc tgtctactcg cctaattacc
//
LOCUS       DAJTYL010000100        13947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYL010000100 DAJTYL010000000
VERSION     DAJTYL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.100"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..297)
                     /gene="csgB"
                     /locus_tag="OO572_003010"
     CDS             complement(<1..297)
                     /gene="csgB"
                     /locus_tag="OO572_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6661934.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSA"
     gene            1051..1701
                     /gene="csgD"
                     /locus_tag="OO572_003011"
     CDS             1051..1701
                     /gene="csgD"
                     /locus_tag="OO572_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6661935.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1706..2095
                     /gene="csgE"
                     /locus_tag="OO572_003012"
     CDS             1706..2095
                     /gene="csgE"
                     /locus_tag="OO572_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6661936.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2120..2536
                     /gene="csgF"
                     /locus_tag="OO572_003013"
     CDS             2120..2536
                     /gene="csgF"
                     /locus_tag="OO572_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6661937.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2563..3269
                     /locus_tag="OO572_003014"
                     /pseudo
     CDS             2563..3269
                     /locus_tag="OO572_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3402..3893)
                     /locus_tag="OO572_003015"
     CDS             complement(3402..3893)
                     /locus_tag="OO572_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6661938.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3995..4549)
                     /gene="ycdY"
                     /locus_tag="OO572_003016"
     CDS             complement(3995..4549)
                     /gene="ycdY"
                     /locus_tag="OO572_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6661939.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4573..5310)
                     /gene="ycdX"
                     /locus_tag="OO572_003017"
     CDS             complement(4573..5310)
                     /gene="ycdX"
                     /locus_tag="OO572_003017"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6661940.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5365..6303)
                     /gene="ghrA"
                     /locus_tag="OO572_003018"
                     /pseudo
     CDS             complement(5365..6303)
                     /gene="ghrA"
                     /locus_tag="OO572_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6538..6625
                     /locus_tag="OO572_003019"
     tRNA            6538..6625
                     /locus_tag="OO572_003019"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6572..6574,aa:Ser,seq:gga)
     gene            complement(6775..7056)
                     /locus_tag="OO572_003020"
                     /pseudo
     CDS             complement(6775..7056)
                     /locus_tag="OO572_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7338..8342)
                     /locus_tag="OO572_003021"
     CDS             complement(7338..8342)
                     /locus_tag="OO572_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6661941.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8414..8974)
                     /locus_tag="OO572_003022"
                     /pseudo
     CDS             complement(8414..8974)
                     /locus_tag="OO572_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9059..9256)
                     /locus_tag="OO572_003023"
     CDS             complement(9059..9256)
                     /locus_tag="OO572_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661942.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9268..9759)
                     /locus_tag="OO572_003024"
     CDS             complement(9268..9759)
                     /locus_tag="OO572_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661943.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9756..10130)
                     /locus_tag="OO572_003025"
     CDS             complement(9756..10130)
                     /locus_tag="OO572_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6661944.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10177..10551)
                     /locus_tag="OO572_003026"
     CDS             complement(10177..10551)
                     /locus_tag="OO572_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6661945.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10714..10935)
                     /locus_tag="OO572_003027"
     CDS             complement(10714..10935)
                     /locus_tag="OO572_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6661946.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10998..11474)
                     /locus_tag="OO572_003028"
     CDS             complement(10998..11474)
                     /locus_tag="OO572_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6661947.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11490..11963)
                     /locus_tag="OO572_003029"
     CDS             complement(11490..11963)
                     /locus_tag="OO572_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6661948.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12305..13126)
                     /locus_tag="OO572_003030"
     CDS             complement(12305..13126)
                     /locus_tag="OO572_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6661949.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13226..13399)
                     /locus_tag="OO572_003031"
     CDS             complement(13226..13399)
                     /locus_tag="OO572_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6661950.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13519..>13947)
                     /locus_tag="OO572_003032"
     CDS             complement(13519..>13947)
                     /locus_tag="OO572_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661951.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3562 a         3416 c         3352 g         3617 t
ORIGIN      
        1 ggcactgccc gcctgatcaa tatatgcaag gttataatct cctgtctggt caatctttgc
       61 ccgattgcta ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa
      121 ctgagcacta ttattagtcc cagcctgacc aattatggct gcctgattaa atgaagactt
      181 actcaattca tttaccgcga agttatattc tgaattagct aaatcataac ctgctgcggc
      241 tgcaatccca ggcgcaccca gtattgttaa catcataaat aacaatttgt ttttcatgtt
      301 gtcaccctgg acctggtcgt acatttaaga aattaaatca tttcaacttg gttgttaacg
      361 caacctgtat tttgttaacg ctgcgttacg atggaaagta tgtctgcgga aatattttta
      421 ataactcacc cgcgcgttgt atttttcttt ttttgttcat accaaagtat taaaacccgc
      481 gtaaatacgc tgttatctac gcaaaaaata ttttgttttc tcttaaatct ccgttttccg
      541 ctatcaaaaa tcaccagaca gtcattcttc ttgcccgtcg ctgattgctg cgatatgtct
      601 tgcgcacaag ccgtgacaaa cctcgtcttt ttgcagtaaa aaaattgtcc acggaggtgt
      661 ggagaaaaaa caaaaacgtt ttacatgacg aaaggactac accgaaatat tttttatatg
      721 tattattagt aagttagcac catttgtatg attttttaaa attgtgcaat aaaaaccaaa
      781 tgtacaactt ttctatcatt tctaaactta ataaaacctt aatgttaaca ttttaatata
      841 atgagttaca ttttgttaca tgtttaacac ttgatttaag atttgtaatg gctagattga
      901 aatcagatgt aatccattag ttttatattt tacccattta gggctgattt attactacac
      961 acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca gctgtcaggt
     1021 gtgcgatcaa taaaaaagtg gggtttcatc atgtttaatg aagtccatag tattcatggt
     1081 catacattat tgttgatcac taaaccttct ttgcaggcga cagctctctt gcagcacctt
     1141 aaacaatcgc tagcaattac aggaaaatta cataatattc aacgttctct ggacgatatc
     1201 tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct tatccattat
     1261 tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct aaatacgcct
     1321 gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt tttttatgcc
     1381 atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgtgg cgaatgctac
     1441 tttacgcaaa agcttgccag ttacctgatt acacattctg gtaactatcg ttataacagc
     1501 acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataagct gcgtatcggc
     1561 gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt taaaacgcat
     1621 ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt ttcctgggca
     1681 aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg tggcggcaga
     1741 atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg gattgttaac
     1801 tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg ataaatggga
     1861 aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat ggggaagctg
     1921 gatcacaata acggtcaatc aggatgttat tttccagact tttttattcc cgttgaaaag
     1981 agactccgag aaaactgtcg tctttgcact gattcaaact gaagaagcac taaatcgtcg
     2041 ccagataaat caggcgttat taagtacgga cgatttggcg catgatgaat tctaaataaa
     2101 aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca tgcttatttc
     2161 gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact ttggtggtaa
     2221 cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt ataaagatcc
     2281 gagctataac gatgactttg gtattgaaac accctcagcg ttagataact ttactcaggc
     2341 catccagtca caaattttag gtgggctact gtcgaatatt aataccggta aaccgggccg
     2401 catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat tgcagttgaa
     2461 cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt tacaaaataa
     2521 ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg cttatttctt
     2581 ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga agccgccaga
     2641 ccgacattaa tgcctcgtgc tcagagctac aaagatttga cccatctgcc agcgccgacg
     2701 ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt taaaccctac
     2761 ccggcaagta acttctccac tgctgttccg caaagcgcca gggcttacaa aacctgctta
     2821 acgagcgcaa ggttattcgt gcggcacaag aaaacggcac ggttgccatt aataaccgaa
     2881 tcccgctgca atctttaacg gcggcaaata ttatggttga aggttcgatt atcggttatg
     2941 aaagcaacgt taaatcgggc ggtgttgggg caagatattt tgggatcggt gcagacacgc
     3001 aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt caatgtgagt accggtgaga
     3061 tcctctcctc ggtgaacacc agtaagacga tactttccta tgaagttcag gccggggttt
     3121 tccgctttat tgactaccag cgcttgctgg aaggggaagt gggttacacc tcaaacgaac
     3181 ccgttatgct gtgtctgatg tcggctatcg aaacaggggt cattttcctg attaatgatg
     3241 gtatcgaccg tggtctgtgg gatttgtaaa ataaagcaga acggcagaat gacattctgg
     3301 tgaaataccg ccatatgtcg gttccaccgg aatcctgaca gataaaaatg ccggaattaa
     3361 taatccggca tttactgttt aagcgaaagc gtgagtgccc ctcacgcttt atttttgatt
     3421 tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca gccacaggcc gctgtttttc
     3481 attgcgtaac caaataccag cccaagcgcc agcgaaggta acaccagttt ccagtcaccc
     3541 tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg gaacaaatga aagcagcagc
     3601 tgtttggcct gaatacacat cagaaacgcg acaataccgg ttatgacata accgagaatt
     3661 tccagatgtg gtgccagtgc actaccgtaa ataatgacca tcgcccacac cacgccacta
     3721 agcagggttg cagcggagat cgccagccct ttcagcccac cttgcgggca agcaaagtag
     3781 gccgtacagc ccaggaagcc cgcccagctt agtaagccca gggaaacagc cacccatccc
     3841 cagataccgg agagaatgcc tgttgtgatt gcaatagaga gaagtatgtt catgccgcgc
     3901 atgatagcag aaaacgcggc catgaatgag atcgagcaca cattttatgc aacaacaaat
     3961 tatctgttgc agttatgagt gacgatgatc acatttactc ttcagaatct tcttccagct
     4021 cgtcccacat tgcactaatg gcatcacggg ttagcggtgc catggtgcgc cagaaaggcg
     4081 tggttgcatg ggcctccact ttgccgagga acgcaccaca ccagggtaac agatactcac
     4141 tgaacagtgt ttccagtgct tcgctctcat cttccgttga ctgatcttcc agccaggaag
     4201 ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc cgccagtagc atccctcgtt
     4261 cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc aacccatgcg ctacgatatg
     4321 gcggcacggc acattcatcg ccgataaaca acgcattgta atcggcagag acttgcgtca
     4381 tatcacagct tttctgtaaa cgcgtcagta actcatcctg ctccagtggc cagttcgcag
     4441 ccagtttccc ctcacgaatc agagtaaaca gcggcaccag taaaggatct tgcggttggc
     4501 ggtaatagag cgaacccagc acacgacaga ggatagaaaa ctcgttcatt aatatatttc
     4561 cattactagt aattaaaggt ctgcaaattc cgcaatcggt gccataccgc gagattcaag
     4621 gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc tctagcggaa aatctaccgc
     4681 gtcgaggatt ttaagacact cttcaaattc ccccatggta aacgcggtgt gagaatccga
     4741 gcctaatgcc acccaacctc cagcatcacg taccgctgca gctaccgcac gacagttgtc
     4801 ttcactgccc ttacgtgagt gtaaaaatga ggaattattg atttccagcg ccacctgatg
     4861 tttcgcggct gcttcagcaa cggctttaac atctatttca tatttcggat ttccgggatg
     4921 gcttattata tgcacattgc cgctggcgat agtggagatc atcgcttgtg tattggtcgc
     4981 tttgtcatgt ggcgcaaaaa ccggctcatg aaaaccggca ataatcagat ccagcgagtc
     5041 aaacatttta ccgctgcagt caatttcacc atcaacattt ttaatgttag cttcaatgcc
     5101 gcgcaggatc cctaccccat caaccactcg cggccagata cgcatgttaa tgaagtgcca
     5161 gtgatgcggc gcatcttcca tatccgggcc atgatcggtg atcgcaaaaa gtttaatgcc
     5221 cttttgtttg gcctgggcaa tgtaatcact caatgtgcta tatgcatgtg tgctggcaac
     5281 ggtatgcata tgaaggtcga cgggatacat aactctctcc tgtattcaat tcccgccaag
     5341 gatagcagga atcctgatgc tttattagta gccacgtgcg cggtcgactt gcccgcagac
     5401 cctctcccct ttttcgagct gggcaatagt gcgagaaatg tactccacag cttcagcggg
     5461 acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt ggatgttgcc agagcggact
     5521 ttcaggcggt aagggttcac gattaaaaac atccagcatt gcgcctttaa ctttcccgct
     5581 atccagcgcc gcgagcaggt catcttccac aacatgaaca ccacgcgcca ggttgaggag
     5641 atacgcgcca tcaggtaatt tttcgagtaa ttgttgatta ataatgccga cggtttcagg
     5701 ggtattcggt aacaaattaa tcaatacccg acattggctc agaaatgcag acagttcttc
     5761 ccgtccggca aagctttgca cgccaggcca cgatttacgg gttcgactcc agcaacgcag
     5821 cggaaagcgc taggtttgca gactctgagc aactttacag cccagtacgc ctgcgcccaa
     5881 aatgccgatg gtaaaatctt cccgatgata ttcaggcagc ggttgccaat gcgaactatt
     5941 ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc agcacctgac tgacagcata
     6001 ttcctgcatt tgctcgccca taccggtatc ttccaggcga aaaagtggaa cagaagggtt
     6061 cagcatttca gggtgtgcct gtagcttgct caaaatagaa tcaacaccgg ccccgagtgc
     6121 gaacaccgct ttaagatcgc gccctgccag catttcaaca ggaggatgcc agactaaaac
     6181 ataatcagca gagtcattat ctccgctttt ccatgccctg actcttgcct gaggaatagc
     6241 tttgcgcagt gcctcaatcc accattgggt atcgaacgtt gggtgataaa agatgatatc
     6301 catactgact cccgaaaagc gttgtgcgaa atttattcgc acttatcgtt atgatctaca
     6361 aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa aagcagattt cgcttattaa
     6421 aaccacacat tgattgaatg tttgaataaa cgcgcgattt tttcaaaaag tttgttgacc
     6481 tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaacg acaatttggt
     6541 gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt gtatacggca acgtatcggg
     6601 ggttcgaatc cccccctcac cgccagatta taagtgcatg ttattgttta acatgcgctt
     6661 ttttattttt ccttccagac agtatgccat cgggtatgcc atctgaaacc ggtttatatg
     6721 ccccgcgatc atatcttatc ggacaatttc cgatctttga gcatactggc aaagctatac
     6781 tgccagcata tcaggatagt gttttgcgat aatatcattc agtctgtcaa ccagttctgg
     6841 ttttctgaac gtgatatgcg cggagccctt cttaaagtat ctgatgctga acatctcatc
     6901 ttcatagctc tctttaccct gaacagactg aatgtgatca tccagacgaa gggtgatgtt
     6961 tccccggttg tcggggattg gtttaccgct gaacaggtgc aacattcttt ccagatcggc
     7021 gagtcggtca gcctgttgtc cggtgataag atgaaatcta tggtcacccc cgtttttgca
     7081 acactgattt ttgattaatg gttggcttgc ttaaatctat ccggcgtctc tgtgggcaag
     7141 gatgcccgcg ccccgatgag ttccgcacct gataatccta aaaatcccct cggcttcagt
     7201 gagccatttc atctgttagg tttttaatca ggtcgatttg ccgttcatgt catcaataat
     7261 cagtcttcgc aaaaccagaa aggtaacttg tatcttattg tttaaaataa actaattgag
     7321 ttatatcagc atctatatca ggcctgatat tcttttttct gagtagtgat tgcccaggca
     7381 attcgagcca gtttatttgc cagagcgcag gtaaccacat tcgaatgctt cttgttgagt
     7441 tgctcccgaa cccactcagc aagttttcca tgcttgcgtt caagtcgcat tatgaatgag
     7501 cgggcacact gaaccagtaa ccggcgtaaa tttttatcac ctcgcttact gatacccaac
     7561 agagtcgatt ttcctccagt gctatactgc cggggaacaa gtcctgtgga agctgcaaaa
     7621 tccctgctgc atgagaactg tttcccgtca ccaagttgcg acgatagtaa gctggcagta
     7681 atcggaccaa caccaggaat tgtcataatg cgctgagcgg tatcatcagc gttgattgac
     7741 tgacttaatt ctgattccag ctctgctatt tgctcaacaa ggtaaaagta gtgggtatgc
     7801 aacctcacca aaaggcggct cagatattcg gggatttcgt gttcagccaa cacgagagac
     7861 aaccgtttta ttacagcatc acctatcggc aggctgattc caaattccag caagaaaccg
     7921 tggatctggt tggttgtttt caccttatct ctgatgagcg attctctgac ccggtgtaac
     7981 gccctcatgg cctgctgggt ctcagttctt ggctggacaa aacgcattga tggtcgtgaa
     8041 gccgcttcgc atatggcttc tgcatcaaca aaatcattct tattactttt gacaaacggg
     8101 cgaacgaact gtggagaaat cagcttagtt tcatgcccaa aatcagagat acagcgagcc
     8161 ataaagtgtg cacccgcaca ggcttccatt acaacaactg agggcggaca tgtcgccaga
     8221 aactgcatca gtttagtacg ggtaaatttt ttacgcagaa gagccttacc agacttgtct
     8281 tgacaatgaa tgtggaagga gtgctttccg agatcgatac caataagtgt gatgttctgc
     8341 atgatggtct ctccgttaaa gggatttacc ttaacagcgt actgctgtta aggtgggggt
     8401 gaccatatca ttatccccac cggtcccatc tcaccagatt gttgacgata attttactgc
     8461 caaatttaca ggggcaattg gttttgtaat tccagttcag ccctctgaag acgttgatca
     8521 cccctcgctc aaacacctca tccttattct ggtgcagttg ttcaaatgtg ctcagaatgt
     8581 tcgcttcgct gatttccgga aaattatcgt actccagtga ccggtaccat gtatcacggg
     8641 cctgcgtatc cattaatgac agcatcccgg aacgttgcat caggtcacgc cagatttcgc
     8701 gatcaagatt ccgggtaatg gctttcattg ctgtttcggg tttctccatc agccagcagc
     8761 cgcagcgaaa atcctgtttc atggcccagt ccggggcagt tttacctccg atgctttccg
     8821 tcagcaccga gatttcagcc agttgtcgga taagtgtgtc gatttgcaac agtgtactct
     8881 ttcgtacagc cagaatgtgg cgaatgctgg ttgagcagat aacgtccgta tgcccgttga
     8941 ccacttccgg acgggagatt gtgatatctg acataatcga tattcctttt tgcagataaa
     9001 tgaaaacgcc agccctgtgc aggctggcgt cagaggaatg aagtctggtg agtgagcttc
     9061 accggttctg gtgcagaagc tgcagatgac gcagggtacg gacatcctcc cgggcctgtt
     9121 cgatacaggg caacagtgct gctgaatccg ttttatcctc atcgttgtca aagataattc
     9181 ccgatccgca gtcgatattg tcctgcagcc acgcaatcag aatatccagc gctgtttccg
     9241 tggttaatga tttcatgttg ttcctggtta ccagtcgaaa gtgggtaaac ccggcaggca
     9301 ttcagcgctg gcatccagat gaatgagact gacgccataa cgccggatga gtgtgacgac
     9361 cagacgacgg aacgtttttg acagccccag gcgtttaagc cgcagaaccg ggtatgacca
     9421 cgcatcggta cgtaacagat atccgctacc tgtaaaatga atccattctg attcaccgaa
     9481 gtcactggtc tggtgtgaca gcgagtacaa ccaggcgttg tccgtttccg tgatatgtgc
     9541 ggtactgcag cgtatgccgg taagggtagc aaatggtggc gtgcattctg ctgaggtctg
     9601 gtcagattca tcaacaacac gaagggagta gccgttatcg cagaccatgt taatcagttc
     9661 agcgagctca ataccatcga cgtcaacgac aatgcgccct atgttcagtg cctgtacgtt
     9721 aacgctgtcg gcttccagtg tcagggccag tttcatcatt tcgcctccgg atacttaccc
     9781 agggtaatgt tatttaccgt tctgtaattg tcgcgggtca tcaggccggt cgcccggcga
     9841 gcccggagga tatcaatact gtttattaac tgagagcggg gacaggtgct gaatcccggc
     9901 tggtcggtac gcaccagcgc gtatttttcc acgagaaaat taactgcatc acacagtgaa
     9961 atgcctgcct caatatgctg ctcaatcaca cgttcatcga caaacggtgt gtcattcagt
    10021 gtgaggccat aatgctggtc cagcagtcgg atgagcagtg tctgccagat ggtgacggga
    10081 gacgggcagc gagatacctc ccgtacatgt gtgtcggata atgttttcat cttaagttct
    10141 ctgtaactgg tagtgctaaa ggtgagagct tcggggttat gaggtggttg cgggtgctgg
    10201 tgtcggataa acagccagat aaacgtaacc acaggagccg agggtgtcgg cttcgcaggt
    10261 cagccctttt gcatacagcg tgacggtatg ctgatatcgg ggattcagtt caccgctggt
    10321 gagcatgagt tccagttgtt tcatcagcag cggaaaggcc tggtccagat ggtatgcatc
    10381 tgcgtcgctg aagcggcctc tgataccggc gcggtcggca aggtaataca ggcggttacc
    10441 ctcttgcacc agacgagcgc caaaacaggg cctcactgtg cagggaagcc cccaccaggg
    10501 gcggtcgtga ttgtcatcgg gatgagttat cccggagagt ttgtctgaca cgataaaatc
    10561 ctctctgaaa agtggttaag aatggtttaa cgatgacagg tcagccggcg cgctgaccgg
    10621 tttcggtgaa gatgtatgca ttgatgagaa tggcctcatc cacagcggcg tctgaggtca
    10681 tctggtcgta ctcgtttccc gggtgacgga acatcagttc agggtgacac tcatcagccg
    10741 gacatacggg ccaaaactgt ccttacggcg ttctgcaaac acggcaagaa cgccgggaat
    10801 atcctgcact tcacgaccag tatacgcttc agcactgccg tgccagcgat acttaccggt
    10861 acagaacgga aaaagacggg atgcaggatg ctgttggtga atacgcatgg cttcaccacg
    10921 ggtgatgatt ctcatgatgt gatacctctg aagacagaag ataaaagtga aaacaggtgt
    10981 gatgtggttg tgacgggtta aagcagacca tgttcggcaa aggagaaaac ctggttgcca
    11041 ccgactatca gatggtccgg cacccggata tccaccaggc ccagtgcctg taccagacgt
    11101 tcggtgataa gccggtctgc cttactgggt gtgacttcac cggacgggtg attgtgcgcc
    11161 agcaccacgg cagcggcatt gtggtacagg gcgcgtttaa tcacttcccg gggatgaact
    11221 tccgtacggt tgatggtgcc ggtgaagagg gtttcaccgg caatcagctg attctggttg
    11281 ttcagataca gcacccggaa ctcttcccgc tccagtcccg ccatgttcag aatcagccat
    11341 tcccgtgccg cacgggtgga ggtgaaggcc acgccgggtt catgaagatg gcggtccagg
    11401 gtttgcaggg cccgcagaat gagactgcgc tcgccgggcg tcatgtttcc gggcagaaag
    11461 gaaagctgct gcattgtccc tgcctccatt cagtcgatga tgcgcataat ggcgctgcat
    11521 tccggatgct gcagggcgta atcccgcagc cggtaataat ggaccgtcat ggcataacac
    11581 tccgtacgac aggcatgatg actgtacgtc atcagacagg cagcaatgcc ggcggcttcc
    11641 gggctcattt cagcgcggtt accgttcatg gcattgaaca gtacccatgt ttcgtcatca
    11701 tcgtcatccg gttcgggtgc cataaatgcc ccgccgttgt tcagggtgta cagattccag
    11761 ataccaccgc agtagtcttc acacagacgg tccatccagc cgaagacacg gggttccagg
    11821 gtgacccact gtggaatgag gccaaaatgc tgcggccaga agctgatgcg ctgttcatca
    11881 gggacgggag tggcaaccag ttgttgcagg ccagtctccg gtgcacaggc cgaagcggtg
    11941 gtgttctgag acaatgtttt catgtatgaa atccttgtaa tgtgaataaa tgagagggag
    12001 taaaggtcgg gagctggcaa catcacgttc atcactgacc gtgatgaccc ggtaatatca
    12061 gggatgaatg atattcggct gaaattccgg acagccgtac gcgtaaccgt tccggtcaca
    12121 gacggatggc cagatgccat tgcctgtata ctcagtctgc acagactgat ttctccggga
    12181 cggtaaagca gacgggtaca ccaggcgcac aggtcatcat cagaaatgga acgacacccc
    12241 ggcagcgcct ccggggcgtc gggtgattct ggtgcctgaa tcacgccttc agggacatgc
    12301 tgcctcagcg caggctctcc agcagcgttt ctgccattac ccacaatgcg cggttgagct
    12361 taatatcggt gtcgatgttg tgaatggcac gggtgtggat acgttttcct tttgcactgc
    12421 ggccggaaat cccgcctttc agcatattct cctggatggt ctgatatgcg ctccacaggt
    12481 ctttaccgta atcctccctg cgtcgtggtg tcagaatgtc agcggtggtg actggctgat
    12541 gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag cgcctggcgt gccggtggcg
    12601 gcaggaccag cgactgcatg gcatcacgct tctcctctat ccggtcaaac acgcccacca
    12661 cctcgtaagc cccttcaata actttctcca ccacatttcc ccggtgtgga acacgcaatt
    12721 ctcccagaga ctgaccgcag accagtccat tggtacaaat ggcacgaaaa tatccgggta
    12781 acatctggta gctggatgaa ccgtcatgag agttgagcag aataatttca gggacatgct
    12841 gaccggttat ctgtccggcc cgccgcagac gcagcatgtg ttttgtgtat tcccggcgac
    12901 ccgggtcacg cacacgtgtc tggcaggcga agaatggctg aaagccttcc cgctgcaggc
    12961 tttccagtac ggtgatggtg ggagtgtacg catagcgttt actgcgggaa gtatgccggt
    13021 cttcaccgaa aatactggga acatagtgca tcagttcttc gtgtgtcagc ggacggtcac
    13081 ggcgtatctg gtttgcagca taaccaaaac gactggctag tcgcataatt tgctccttat
    13141 cggtggttaa gatttactgg tgtaataaat gaaaaagcca cgtctcccgg agaagacgcg
    13201 gcctgatgga cgaaattaat gaggtttatt gtctgagaag accgtcactg gcgaaccttc
    13261 cggcgtcagt ctctgcgttg cggataaccc gacggaaatg agttccctgg tcatcgtcga
    13321 taaacacatt gcggtaagct gttgtgatga caacagcctg ctcgcgtgag aaagggactt
    13381 cgggcaataa acgtgacata caaccagacc tgtatttggt tccctgaaaa tataagtccc
    13441 tgtcatcatg gcagagccgt atttactttt gatatcaata aattaataac gcgaagatga
    13501 accgcataac ttacgccttt aatttgtttt gtttacgtag tccctgacca ggtacgcgaa
    13561 ttcgtgaacg ccagtttcac caaaccaaat caggtaacca gggtcgatgt tgtaatagta
    13621 accaacgact ttatgcgttt ttgggttata gagtgttacg atatagtccc agccgcaggc
    13681 gtgggcttta caaccgccac cttctaccca ggtcgttccg ttaatgttta caggatacgc
    13741 accaccatca ggaccatcca tatagccgtc ttccccaaac attccgttta acccaacagg
    13801 gtttaatgct ttgtcgatag cagctctgta ggtagcgtca ccttcgtata actcgtacgt
    13861 tgttggacat gcaccaggct ggtcagcccg ggcttttgag tcgcatttaa gtgtctgatt
    13921 tacagtggcg gcgtttgtgt caggact
//
LOCUS       DAJTYL010000101        13850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYL010000101 DAJTYL010000000
VERSION     DAJTYL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.101"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1273)
                     /locus_tag="OO572_003033"
     CDS             complement(<1..1273)
                     /locus_tag="OO572_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6661952.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAI"
     gene            complement(1316..2690)
                     /locus_tag="OO572_003034"
                     /pseudo
     CDS             complement(1316..2690)
                     /locus_tag="OO572_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2736..4772)
                     /gene="yihQ"
                     /locus_tag="OO572_003035"
     CDS             complement(2736..4772)
                     /gene="yihQ"
                     /locus_tag="OO572_003035"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6661953.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            complement(4971..5873)
                     /locus_tag="OO572_003036"
     CDS             complement(4971..5873)
                     /locus_tag="OO572_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6661954.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(6011..7252)
                     /gene="yihS"
                     /locus_tag="OO572_003037"
     CDS             complement(6011..7252)
                     /gene="yihS"
                     /locus_tag="OO572_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6661955.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(7269..8147)
                     /gene="yihT"
                     /locus_tag="OO572_003038"
     CDS             complement(7269..8147)
                     /gene="yihT"
                     /locus_tag="OO572_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6661956.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(8171..9067)
                     /gene="yihU"
                     /locus_tag="OO572_003039"
     CDS             complement(8171..9067)
                     /gene="yihU"
                     /locus_tag="OO572_003039"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6661957.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            9235..10131
                     /gene="yihV"
                     /locus_tag="OO572_003040"
     CDS             9235..10131
                     /gene="yihV"
                     /locus_tag="OO572_003040"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6661958.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            10165..10950
                     /locus_tag="OO572_003041"
     CDS             10165..10950
                     /locus_tag="OO572_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6661959.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            11049..11648
                     /gene="yihX"
                     /locus_tag="OO572_003042"
     CDS             11049..11648
                     /gene="yihX"
                     /locus_tag="OO572_003042"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6661960.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11642..12514
                     /locus_tag="OO572_003043"
     CDS             11642..12514
                     /locus_tag="OO572_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6661961.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12511..12948
                     /gene="dtd"
                     /locus_tag="OO572_003044"
     CDS             12511..12948
                     /gene="dtd"
                     /locus_tag="OO572_003044"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6661962.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            12993..>13850
                     /gene="fabY"
                     /locus_tag="OO572_003045"
                     /gene_synonym="yiiD"
     CDS             12993..>13850
                     /gene="fabY"
                     /locus_tag="OO572_003045"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6661963.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETP"
BASE COUNT         3389 a         3516 c         3636 g         3309 t
ORIGIN      
        1 taattgccag tgcacaaggc cagataaaaa tgagctgacg caaaccctgt aatgtcgcat
       61 cgctttgcgc gatgttggga acataaccaa tttgcgtcag cataatgcct ggcaagaagc
      121 cagcaagtgc ggcagaaatt ttacgaaaga aggtataacc ggtataaaca gaaccttcgg
      181 cacgaatacc ggttttccat tcaccgtaat ctacggtatc tggtaccagt gcccagttca
      241 ggctgtttac aaaggccgtg ccaaaaaagg cgacacaaga gaacatgacg aaagtgaaag
      301 agttacttcc ccagaaataa ttcagtatat cacccaccgc ccatagcacc atgccagcta
      361 aataaacctg ttttttacca aaacatttta cagttactgg caccagtaat acgccgacga
      421 ggatgcatcc catactgaag aatcccatcc acgacaataa attaatatca ttcagcacat
      481 actgggtgta atagacctga atcgccagtt tgatattaaa tgccgccaga gtacacaggt
      541 tagcaatgca taaaaccagc aatggtggat tacgaaatat cgcgcaaaaa gatttgagaa
      601 tactggcttt atgtccggtt ggcgctgtgt ccacataatg ctctttgacg cctctgtagc
      661 acagcatcat aaaaataaag ccgccaatgg agaacataag tgccgcacag gcataaccga
      721 cggttgagtc agaaaaaagc gactgcaacg gaataaacgc cacggtacaa atcaatagcc
      781 ctatggttgc accaccctgg cggtaagccg cgagctgcgc gcgctcattc gggtttttgg
      841 taattgccgg gatcatcgca ccatacgagc agttcatcag actgtatgaa agtccaaaca
      901 tcataaacag cgtggtggca atcgtcgttt taaccggcaa acaaaaggtg gtggcgataa
      961 actggagcgt tgcaattaac gccgccggaa ctgctgcata taaaataaag ggacgaaatt
     1021 tgcctttagg cccaatattt ttccgcgagt cgagtaaaaa tccggtgagc atatcggtaa
     1081 acgcggtaaa aaacttcgcg accagaaaga taatcccgcc atagtaagca ggcatcccta
     1141 attcatcggt ataaagtttg aggagataca gagtaccgat gcagagcatt aaattcgaac
     1201 cgacgtcgcc cataccatag gcgatttttt cccgcagatt cagttttaat gtcagtggat
     1261 tatggtcaga cataatcact ccttataacg ccccgcaggg cgtcgtttat tattattacg
     1321 ccgtgcgttt acgggcttct atctcttcca caatgcggac atacatcttc tcgttcaggc
     1381 tgtagaagca acccatcgcc acaatggtga ctaccgccag tgcgcttggg tagatgaaga
     1441 tcaactggcg taacccttca atggtgtggt cagcctgtgc gacgttcggc acatagccaa
     1501 tttgcgtcag catccagccg gggaagaaac cagccagcgc ctgagacact ttgcgaaaga
     1561 aggtgaagcc ggtgtagacc gtgccttccg aacgcacgcc ggtgcgccac tcgccgtact
     1621 cgacggtgtc ggaaaccagc gcccagttca ggcgctgagc cgaagaacgc caggcaggag
     1681 aacgccacaa agctgaccga gccgccgccg aagaaatagt tgagcagatc gcccagcacc
     1741 caaatcagca ggccgccgat atagaccttc ttcttaccaa aacgtctgac tgcgccaggc
     1801 atcaggaaca cgccgatgaa aatacagccc atgctgaaaa atcccatata cgacaacagg
     1861 atgggatcgt taagtacgta ctgggtgtaa tagacctgaa tggcgagctt gacgttaaac
     1921 gcccctaagg tgcagaggtt ggcaatacac agaatgaaca gtgggcggtt accggcaatt
     1981 gcgcggaaag attgcaacag gccaggcttt tgcgccggat tggttggttg ggtttcgaca
     2041 taacgctctt tcacgcctga gtagcagatc cacataaaca gcaggccgaa cagtgaaaac
     2101 agcgtggcgg cgaagatata gccaagtttc tgattacctt cgataagatt cataactggc
     2161 acgaatccca ccgtgcacag cagcaggccc agcgtagcgc cgccctgacg ccatgccgcc
     2221 agtgaggcgc gttcgttggg gtttttggtg attgccggga ccattgcgcc gtaggagcag
     2281 ttcatcatgc tgaaaaacag tccgtaaagc ataaacagaa tagtggccat caccgtttta
     2341 ccggtgacat caaacggtgt gccgacaaag ttggcgatcg ccagtaaggt gaccgggaat
     2401 gacgcataca gaataaaagg acggaactta ccttttggac cgatcttgcg tcgggaatcc
     2461 aacataatgc cggtacccat atcggtaaac gcagtaaaga attttgaaat caaaaagata
     2521 atgccgccat aggtgcctgg cagccccaga acgtcggtat aaaacttcaa aagataaagc
     2581 gtaccgatat ccagcaggat gttagaggcc aggtcgccaa taccgtaaga gagtttctct
     2641 ttaaagggca ggcgtaaagt tgctggatct tccgttgtga tgtgactcat cgatgttctc
     2701 cctttgccca tgagtttccc cacgggcgga tgtgattaga tgctgtttaa cgacgcgaac
     2761 agtgccgccc attcgctatc ggcgcgataa aagaccggcg gcttgccgat gggcgcatta
     2821 acggtaactt ccccgccccg gaacgcttca cccgtccagg catgtaccca gttatcctcc
     2881 ggcagataga gcgtccagtc gctgcgacct tcttcgtgta ccggagcgac cagaatgtcg
     2941 cggcctaaca ggtactgata tttcagggaa taggtatgtg catcgtcttc gtaatgcagg
     3001 aacagcgggc gcatgaccgg cagaccggac ttcgcattca gcgcgacggc ctctttcaga
     3061 taaggtttca gggtggtgaa aacggtagtc atacgggcaa aatgggcgat agtttccgca
     3121 tcgccgtcaa actgccagtt gtcgccagga cggttacctt cgtgggtgcg catcatcggc
     3181 gtgaaggcgc tgaaatcgca ccagcgcagc agcagctctt tgctgcgctt catctcaaac
     3241 agggtggtgt aaccgccaat gtcgctgtga tgcagaccat gtccggtcat tgccagcgac
     3301 agcgccgccg ggactacgga cgccaggcca tcgtcgagac tccagtccac gttctggtcg
     3361 cccgcccaca tcatggtgga gtatttctgg ctaccggtag aaccggcgcg cataaagaag
     3421 aggatctcgc cgagcttgcc cgtttcttca agggcttcgt agttacactt cgcccacagc
     3481 gcaggccagg cgttatgcat aatttcggca ctgacgccgt tatgcaggta cgtgtcggtg
     3541 ggcagatact cgccgaagtc agccatccag ccgccgcagc cgagttcaat catgttcttt
     3601 ttgatcactt ccttgaacca ggcgtaggct tctggattag tgagatcgac aacgccgccg
     3661 taaaactcgc caaactccac cagatagtca ccgccagagg catcttttgc tagatagccg
     3721 tgttgtgccg cttcttcgca gagatcttta tcgctggcaa catacgggtt gatataggcc
     3781 aggaactgca cgccctcctg ataccactgc ttaatgcgtg aatccagttg cgggtagttt
     3841 tcgctgttcc acttccagtt ccacatcacg cgcttgccaa aagaggttat acggatccca
     3901 gaccagtcct gcgcccagat gccgttaacc ttcacgcccg cgttacgcat ggtgtccagt
     3961 ttcttctggc acacttccgt cccgccctga atgccgagcg ttaccccgtc ataaatccag
     4021 tcgggcagtt ctggctggcg tcccagcagg gcagttaatt tttccagcag ggagatgtat
     4081 gtgtcagcac attcaaaacg tagcgtagct ttgtcttccc acagcgccag ttcatggtat
     4141 tccggggcgc taaagtcgaa gttcatgtag caactgttat caacatggca gtaatacttc
     4201 tgcgtgctga caaacgtagg ctgtgggaag aaagtccagt aatagtcgcc gcccgcattt
     4261 tctttgcagt cggcctgcca ggtgacatag gtttgtttgt tgcgaccaac gccttgttca
     4321 ctggtccata gcgggaacgg tttgccgcgc agatcgaagt aggaaaactg ttcgccgcag
     4381 ccgtagatat ggtcctctgg ttgagcggca aggcgcagcc agatacggtt gtggttaagg
     4441 ttgtcgtttt gtagttccag caataaacgg ccctgatcgt cggcagagat attcagcgtg
     4501 gcgctaatgt cagaaccacg gctgaaatga attaaccaac catccggtga ctggctgacg
     4561 atggcgtcgg taagcgcaat tttctcctgt agtttatctt taatgctgaa attaccgcgg
     4621 aacatatcga tatccgctat acctgagcca atccataaac aaggattatc tttgttatgg
     4681 gttaaaataa gacgttgttg aaaatgtagg gtaaaactgt cgttattctg atgaaattga
     4741 aaatctaata actgtggacg tggcgtatcc ataactaatc tccatagagt tgagaacgag
     4801 attgttgcct tcctgttatt acaggcttat gtaaaatgat tctcataaaa ctgatatata
     4861 aaaactttac agaagctgag ctgtttttaa cttaataaat tgaagataca taatgttgcc
     4921 tttagcgagt gaaatgttct tcaatgattt tgactgctaa tcaatacaag ttattttctc
     4981 tgtccgtaaa tattaaaaaa caggcggtgc ggttttcctg gttccagtaa taataagtcg
     5041 atacctgaat taaaggcgtt aggcgggcaa ctcataggtt caactgccag tccctgacgc
     5101 tgtaattttt caccgctata aacctgcacc cagggttgat cggaacaaag gcttaccgaa
     5161 agcgcctgct gtggatgggt aatggtcatt tcccacaaat cattagcggt tttaaaggtg
     5221 tggtcgattt ttgtcgcggc tatttttttc gcctgcgtga aatttaaatc cagctcatca
     5281 acgtggtgaa gcgttgtcgg atttacgtgt tcatcgacgg cataaacctg gtttgccgga
     5341 agctggaaca gatattcatc aacagatgtt aagttgcagg ttaaataagg atgaataccc
     5401 acgccataag gcgctgcgac agtgccgata ttctggcttg cgatctcaac ggaaagaccc
     5461 gtgtgtgcgt tgagcgaata aacaacttgt gaagccagca taaacggata gccgtaactg
     5521 ggcggcagaa aagctgtcag ggtaacgctg gtggtggtta attcactaat ttgccagtct
     5581 cgccaggcaa gcagcccgtg aatagcggct tttgaactat gttcattaat gggtaactga
     5641 tattcctgcc cctgataacg gtaacagccg ttggcgatcc ggttcggcca cggaataagc
     5701 actttaccca gatgcgccag cggcatctct tcgggtttgt gcgggatgac taaatgacaa
     5761 ccctgaaagg ttaattcagc cagaccagca ccgacggtca caatggtcgc gtgataatcc
     5821 cccgcggcaa gtgaaacggt ctgtccactg caatgcatat tggtgatttg catacaaggc
     5881 accttaatta acgacataca gcctcccgtg aggctgatgt caaacccaaa aactgcaccg
     5941 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaaaaccc gcaccctatt
     6001 ttcagatcag ttatttcgca ttaatatcca gcagacccgc tgcaaccgct ggagccatgc
     6061 cgggcgctaa cgggatacgc gggatcacca ggcaatgcag caggtgataa atatcctgtt
     6121 tgccgtccca gactttggtg atgaccttat tgtccgcatc cagctcctgc caccaggaac
     6181 cattttcata gtccatcagg tacttaatgc agtactccca ccatgtttga taccaggttt
     6241 catactggcg atcgccagtg acggtgtaga gcgcgtagac cgtacccatt gcttcgacga
     6301 taggccaacg tacacgttcg cggaccaccg gttttccttc ccagtcaacg gtataaacaa
     6361 tcccgtccgc tccatcgggt gcccaggcat cgcgcacggt ggcgttaaac agacctttgg
     6421 catcttctag cagccatgct ggtggttgtt cgcaacgggc ttccagggca gcatggatgt
     6481 gcagcattaa acggccccat tcgatccagt ggcctggtgt acctccgaac gcgcggaagc
     6541 gatgcgccgg gttatctttg ttgtaatccg gcagcggatt ccactgggta tcgaagtgtt
     6601 cgttaacgcg ataatgatta tttctggcga cgtcgtggat aatcacggaa gccacgcgaa
     6661 tcgcgcgatc cagccatttt ttgtcgtgag tgacgtcata aacaatcaag aaagcttcca
     6721 ccgcgtgcat attggcattg ccgccgcggt actcttcggt tttgctgaag gcttcgtccc
     6781 aggactccag gcacatctgc tcttcttcgc tccagaaata tttctcgata atctcaatgg
     6841 tgtaatcgag cagcttgcgc gcttccgggt gacccgttgt gacggcgctg gcggcaccca
     6901 gcagagcaaa gaagtgttga tagccctgtt tggaggcatc caccacgccc tcgtcattca
     6961 cgcaggcata ccagccgcca tattttttat cacgcagtgc gccgttcatg gctttaatac
     7021 cgtgatcaac caacgcgtaa gcgccaggtc gacccatcgc cgcagcaacg gaataaacgt
     7081 gcaacatacg agctgtgatc cacagatggg tgcccatctc ttctttgatt tgccctttat
     7141 tgcctaacca gccaaaacca gtcggcacta cggaattttt accaaaatca aagatgcggt
     7201 cggtttcctg ttccagccaa cggttgtggc ttagggtgtt aaaccatttc attcctttta
     7261 tcctcatctt agcggcgttt agccatcatt tcgtcgacga tatcgccaag ttgttgtaat
     7321 ttcggtgcgc aaacgtcacg gagcatcagc tcgttgtctg gtaaaccgac gaccgatgcc
     7381 cagactgcac ggcctgccag gaatcccgat gcgcccgccg tcattgccac gcgtacggca
     7441 cgcgggaaca gtttttcgtc gacactggaa gagaggatca cccatggcat attgatatgg
     7501 tcattcagac gttgtgaagc acagagaagc tcttgttgcg gacctttgcc ataaagcggc
     7561 atttcaactt tgtagaggtc agcgccactg tcgcccagct ctttggcggc atcgatgatc
     7621 gcttgttcgc gatcgaattt atcgccacga cgcggtgggc ggacgactgg ctcaatgatg
     7681 cttaccagac cgtgtgagtg gcacagttcg ttgaactctt tcaccatatc cagacgttgc
     7741 tgcgcatctt catcactacg ccacagcacc agcagtttta aggctttgcc accgtcttgt
     7801 ttgatctgta gcggattgat tttgcggtca ataaccacgc tatcgaccgg aataccgttg
     7861 ccaggaatga actcatcggc ggcgacaatc atggcgcaac ttttggcaat cgcgttttgc
     7921 tcaaccacct ggcggtagca gaattgttga tctaccagaa tcgccgaggc ataaggcgag
     7981 agggcctttg cagcgttaac tttgaaatca gttaaaacgc tatcggctac cggagcgggt
     8041 gccccagccg cggcaaacat catgcgcatg gcttcgcgct gatcgaccgc cagcatggca
     8101 aaaccgcccg atgcgcgcgt aatgtcgttg atggtgtact tattcattca tttattcctt
     8161 tatccagtcg ttacattttt actttggcag tcatcccggc actgacacgg acctgttcca
     8221 gaatggcgga ccagtcctgg cgaccgcgac ccgctgcgcg cgcctggcta taaacctccc
     8281 gtgaggcggc ccccagcggc attggcacat gcagctggtt ggcgacatca agggcgatgc
     8341 caagatcctt atgggcaaga tcgatcatga aggcgggaga aagatctccg ctgaggactt
     8401 tgtttggcca ggaagtggtg aagtgacctt taccggcggc ggtaccgctc atcactttga
     8461 cggcaacatc gaagggaaga ttcagggctt cgcacaaaac ggcggcttct gccgaaagcg
     8521 cattgagcgc gatgctcatg tagttgttga tgagcttaac gcggatcccc atgcccggac
     8581 cgccagcgtt gatcaactca ctgcccatcg ccatcaggat cggtgtggca cgttcaactt
     8641 gttcagcggt gccgccagcc agcagtaaca gagtaccggt aatggcattt gcagaagtac
     8701 ggcctaccgg aacatccatc atgctgaagc ctttggcttg catatcggca atcaatttat
     8761 cggtttgcag cggatggatg gtggacatat caatgaccag cgcatcggta gataagcctt
     8821 cgcaaacgcc gttttcaccg aacaacacgt tgcgcaccag atcgccattc ggcaacatgg
     8881 taatgataaa ttcggcatct ttagcggcct gcgccgggtt ggcggcggga gtcgcacctt
     8941 tgtctaccag atgccgcaca gcttcggcgt tcacatcaaa gacgcgaagt tgatgccctt
     9001 gctgcaataa attgctcgcc attggcgaac ccatttgtcc taaaccgata aacgcgattg
     9061 ctgccataac cctctcctga atacagtgat gtcacttttt gtcatttatg acatgctttg
     9121 cttgtctgtt tttgatcgaa tttgtaattt atcgtcaaaa aattgacagc cgtcactttt
     9181 taaacaattg gtgaaattaa aatgaacgca tcccaaaatg tttaaggaat gaccatgatt
     9241 cgtgttgctt gtgtaggtat aaccgtgatg gatcgcatct attacgtgga agggttaccg
     9301 acggagagcg gtaaatacgt ggcgagaaat tatacggaag ttggtggcgg gccagcggcg
     9361 acggcagcgg ttgcggcggc aaggctgggg gcgcaggtcg attttattgg tcgcgtaggt
     9421 gatgacgacg cgggcaacag cctgctggca gagctggaat cctggggcgt taacacccgt
     9481 tacaccaaac ggtataacca ggcgaaatct tcgcaatccg ccatcatggt ggataccaaa
     9541 ggcgagcgga taatcattaa ctaccccagc ccggatctgc tgcctgacgc agagtggttg
     9601 gaggaaattg atttctctca gtgggatgtt gtgctggcag atgtacgctg gcacgacggc
     9661 gctaaaaaag ccttcaccct ggcccgtcag gcgggtgtga tgaccgttct ggacggggac
     9721 attacgccgc aggatatcag tgagctggtg gcattaagcg atcacgcggc cttttcagaa
     9781 ccgggtctgg cgcgcttaac gggcgtgaaa gagatggcca gtgcgctaaa acaggcacaa
     9841 acgctcacaa atggtcatgt ctatgtgacc cagggtagcg caggctgcga ctggctggaa
     9901 aatggtgggc gtcagcatca accggccttc aaagttgatg tggtagatac cacaggtgcg
     9961 ggagatgttt ttcacggcgc tttggcggtg gcgctggcaa caagtgggga tttagcggag
    10021 tcagtccgct tcgccagcgg tgtagcggcg ttaaaatgca cacgtcccgg tggacgagcc
    10081 gggatccctg actgtgatca aacccgatct tttttgtcac tttttgtata aaatgccagg
    10141 gtgatggttt ttcgaggaat tttcatgagc cttaccgaac taaccggtaa cccgcggcac
    10201 gaccaactcc tcatgctgat cgccgagcgt gggtatatga atattgatga gctggcaaat
    10261 ctgctggatg tctccacgca gacggtccgc cgggatattc gtaaattaag cgagcaaggc
    10321 ctgattacgc gccatcacgg tggcgcgggt cgggcttcca gcgtcgttaa tacggcgttc
    10381 gagcagcgtg aggtttcgca aaccgagaaa aaaaaagcga ttgccgaagc ggtggcagac
    10441 tatattcctg atggttcaac aatatttatc accattggta cgactgttga gcatgttgcc
    10501 cgggcgttac ttaaccataa tcatttgcgg ataatcacca acagcctgcg tgtggcgcat
    10561 attctttacc acaacccgcg ctttgaagtg atggtgcccg gcggtacgtt gcgctctcat
    10621 aatagcggga tcattggccc ttcagcggcg tcctttgtgg ctgattttcg tgctgattat
    10681 ctggtaacaa gcgttggggc gattgagagc gatggcgcgt tgatggagtt tgatgtaaac
    10741 gaagctaacg tggtgaaaac gatgatggcg cacgcgagaa atattctgct ggtcgccgat
    10801 cacactaagt atcatgcttc ggcggcggtt gaaattggta acgtggcaca ggtcactgcg
    10861 ctctttaccg acgagctgcc gcccgctgcg ctaaaatcac gcttacaaga cagccaaatt
    10921 gaaatcattc ttccccagga agacgcgtag atttttgtga ccgttaacct ggcttcatac
    10981 cttgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg gcgcgaaagg
    11041 aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc gactttaacc
    11101 gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt aagaagagtt
    11161 ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac gaagcgttcg
    11221 cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc tctcacggct
    11281 ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat aaactgcgtg
    11341 agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc accttctggc
    11401 cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg caagatctgg
    11461 ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa ggtttttcac
    11521 ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagct aatcagctag
    11581 gcattaccag tattctggtg aaagataaaa ccaccatccc ggactatttc gcgaaggtgt
    11641 tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat gggcctggct
    11701 aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag gtaaccttgc
    11761 ctatgtgtcg ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc ttttcgccgc
    11821 ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg ccaactttct
    11881 gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcta attccaacaa
    11941 gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga tgtactccat
    12001 cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa tttactcgtt
    12061 cgccgtgtac tggatgattt taacgctggg gccgctgctg gcaggggcca gtctggcgat
    12121 cagttcctat ttgctctccc tgcgctgggc gagcgatctc aatactgtca tcgacaacgt
    12181 gctgcgtatt tttccgttgc tgttgtcgtg gatctccttc tggttgctgt acagcattgt
    12241 tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg ccgcactcct
    12301 gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt catatcagct
    12361 catttacggt gtgctggcgg tgatccccat tctctttgtt tgggtctact ggacatggtg
    12421 tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca aactaaaaca
    12481 agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg cgtaacccgt
    12541 gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact tttggtgtta
    12601 ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga gcgtgtgctc
    12661 ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca acaggcgggc
    12721 ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg ggggatgcgc
    12781 ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga ctatttcgtc
    12841 gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc cgatatgcag
    12901 gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc cagcttccag
    12961 ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcaggtt ccacaaacag
    13021 aagaagagtt agagcgttac tatcactttc gctgggaaat gttgcgtaag cccctgcatc
    13081 aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag atggtcgtcg
    13141 acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac aatgaagcgt
    13201 ccattcgctt tatggtcgtt cacccggacg tgcaggacaa agggttagga acgctgatgg
    13261 cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc tgtagcgccc
    13321 gtgaagacgc ggtggagttt ttcgccaagc tggggtttgt taatcaggga gaaatcacca
    13381 cgccaaccac cacgccgatt cgccattttt tgatgattaa gcccgtcgcc actctggatg
    13441 atattttgca tcgcggcgac tggtgcgcgc agctgcaaca ggcgtggtac gaacatatcc
    13501 cgcttagtga aaaaatgggc gtgcgtattc aacaatatac cgggcaaaaa tttatcacca
    13561 ccatgccgga aaccggcaat cagaatccgc accatacgct gtttgccggg agtttattct
    13621 cgctggcgac gctcaccggt tgggggctta tctggctgat gctgcgtgaa cgccacctcg
    13681 gcggaacgat tattctggcg gatgcgcata tccgctacag caaaccgatt agcggtaaac
    13741 cccatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg gcgcgcggac
    13801 gaaaagcacg ggtgcagatg caggtcgaaa tctttggcga cgagacgccg
//
LOCUS       DAJTYL010000102        13474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYL010000102 DAJTYL010000000
VERSION     DAJTYL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.102"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            36..3560
                     /gene="nifJ"
                     /locus_tag="OO572_003046"
     CDS             36..3560
                     /gene="nifJ"
                     /locus_tag="OO572_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6661964.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            3927..5056
                     /gene="ompN"
                     /locus_tag="OO572_003047"
                     /pseudo
     CDS             3927..5056
                     /gene="ompN"
                     /locus_tag="OO572_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5197..5631
                     /gene="uspF"
                     /locus_tag="OO572_003048"
     CDS             5197..5631
                     /gene="uspF"
                     /locus_tag="OO572_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6661965.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            5807..6742
                     /gene="ttcA"
                     /locus_tag="OO572_003049"
     CDS             5807..6742
                     /gene="ttcA"
                     /locus_tag="OO572_003049"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6661966.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(6871..8238)
                     /gene="dbpA"
                     /locus_tag="OO572_003050"
     CDS             complement(6871..8238)
                     /gene="dbpA"
                     /locus_tag="OO572_003050"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6661967.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8716..9699)
                     /gene="zntB"
                     /locus_tag="OO572_003051"
     CDS             complement(8716..9699)
                     /gene="zntB"
                     /locus_tag="OO572_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6661968.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            9954..11186
                     /gene="dgcM"
                     /locus_tag="OO572_003052"
                     /pseudo
     CDS             9954..11186
                     /gene="dgcM"
                     /locus_tag="OO572_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11207..11770)
                     /gene="smrA"
                     /locus_tag="OO572_003053"
                     /pseudo
     CDS             complement(11207..11770)
                     /gene="smrA"
                     /locus_tag="OO572_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12024..>13474)
                     /locus_tag="OO572_003054"
     CDS             complement(12024..>13474)
                     /locus_tag="OO572_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6661969.1"
                     /translation="SMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDEMA
                     WLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILFRA
                     LQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLDWR
                     WLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEGAR
                     WGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGGVG
                     IFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGL
                     GGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASG
                     VFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRSR
                     SDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
BASE COUNT         3600 a         3377 c         3196 g         3301 t
ORIGIN      
        1 gtgcgcccct catttgcgca atgtaagggt gtcatatgat tactattgac ggtaatggcg
       61 cggttgcttc ggtgacgttt cgcatcagtg aagttatcgc catctaccct attaccccca
      121 gttccacgat ggcagaacag gctgatgcct gggccggaaa cggcttgaag aacgtttggg
      181 gagacacacc acgcgtggtt gaaatgcagt cggaagcggg tgctatcgct accgtgcatg
      241 gcgctttgca gacgggtgcc ctttcaacat cgtttacgtc atcgcagggt ttgctgctga
      301 tgatcccgac gctgtacaaa ctggcaggcg aactgacgcc atttgtcctg catgtagcgg
      361 cacgtaccgt tgccacacat gcactttcta tttttggcga tcattccgac gttatggcgg
      421 tgcgccagac gggttgcgcg atgttgtgtg cggctaacgt ccaggaagcg caagactttg
      481 ctctcatttc gcacatcgca acgctgaaga gccgcgtgcc atttattcat ttctttgatg
      541 gttttcgcac gtcccacgaa atcaataaaa ttgtcccgct ggccgatgac actattcttg
      601 atctcatgcc gcaggccgaa attgatgctc atcgcgcccg ggcgctcaac ccggaacatc
      661 cggtgatccg cggtacgtcc gccaatcctg acacttattt ccagtctcgc gaagccacca
      721 atccctggta caacgcggtc tatgaccatg ttgaacaggc gatgaatgat ttcgctgccg
      781 cgacaggtcg tcagtatcag ccgtttgagt attacgggca tccgcaagcg gaacgggtga
      841 ttatcctgat gggctctgcc attggcacct gtgaagaagt ggttgatgaa ttgctaaccc
      901 gtggcgaaaa agttggcgtg ctgaaagttc gtctgtaccg tcccttctcc gccaaacact
      961 tgctgcaagc tctgccggga tccgtacgca gcgtggcggt actggacaga accaaagaac
     1021 ccggtgccca ggcagaaccg ctctatctgg atgtgatgac cgcactggca gaagccttta
     1081 ataatggtga gcgcgaaacc ctgccccgtg tcattggtgg gcgctatggt ctttcatcca
     1141 aagaatttgg cccggactgt gtactggcgg tatttgccga gctcaacgcg gctaaaccta
     1201 aagcacgctt tacggttggt atttacgatg atgtgaccaa tctgtcactg ccgttgccgg
     1261 aaaacactct gccaaactcg gcgaaacttg aagccttgtt ttatggcctt ggtagtgatg
     1321 gcagcgtttc cgcaaccaaa aacaatatca agattatcgg taattccacg ccgtggtacg
     1381 cacagggcta ttttgtttac gactccaaaa aagcgggcgg cctgacggtt tctcaccttc
     1441 gcgtgagcga acagccgatt cgttccgctt atctcatttc ccaggctgat tttgttggct
     1501 gccaccagtt gcagtttatc gataaatatc agatggctga gcgtttaaaa cctagcggca
     1561 ttttcctgct taatacgccg tacagcgcag atgaagtgtg gtcgcgcttg ccgcaggaag
     1621 ttcaggccgt gttaaaccag aaaaaagcgc gcttctatgt gattaacgcg gcgaaaatcg
     1681 cccgcgaatg tggcctggcg gcccgtatta ataccgtcat gcagatggct tttttccatc
     1741 tgacgcaaat tctgcctggc gatagcgccc tcgcagaatt gcagggtgcg attgccaaaa
     1801 gttacagtag caaaggtcag gatctggtgg agcgcaactg gcaggctctg gcgctggcgc
     1861 gtgaatccgt agaagaagtt ccgttgcaac cggtaaatcc gcacagcgcc aatcgaccgc
     1921 cagtggtttc cgatgccgcc cctgatttcg tgaaaaccgt aaccgcggcg atgctcgccg
     1981 ggcttggtga tgccctcccc gtttcggcgc tgccgccaga cggcacctgg ccgatgggca
     2041 ccactcgctg ggaaaaacgc aatatcgccg aagagatccc catctggaaa gaggaactct
     2101 gtacccaatg taaccactgc gtcgccgctt gcccacactc agctattcgc gcaaaagtgg
     2161 taccgcctga agcgatggaa aacgcccctg ccagcctgca ttcgctagat gtgaaatcgc
     2221 gtgatatgcg cgggcagaaa tatgtcttgc aggtggcacc ggaagattgc accggttgta
     2281 acctgtgcgt cgaagtttgc ccggcgaaag accgtcagaa tccagagatt aaagccatca
     2341 atatgatgtc tcgccaggaa catgtcgaag aagagaaaat caattacgat ttcttcctca
     2401 acctgccaga aatcgaccgt agcaaactgg aacgtattga tattcgtaca tcgcagctga
     2461 ttacaccgct gtttgaatat tcaggtgctt gccccggttg tggcgagacg ccgtatatta
     2521 aattgctgac tcagctctat ggtgaccgga tgttgatcgc taacgccacc ggctgttctt
     2581 caatttatgg cggtaacctg ccctctacac cgtataccac cgatgccaac ggtcgtgggc
     2641 cggcatgggc gaactctcta tttgaagata atgccgaatt tggccttggt ttccgcctga
     2701 cggtcgatca acaccgtgtc cgcgtgctgc gtctgctgga tcaatttgcc gataaaatcc
     2761 cggcggaatt actgacggcg ttgaaatcag acgctacgcc agaggttcgt cgagaacagg
     2821 tcgcagcttt acgccagcaa ctcaacgatg ttgccgaagc acatgaactt ctgcgtgacg
     2881 cagatgcact ggtggaaaaa tcaatctggc tgattggtgg cgacggctgg gcttacgata
     2941 tcggctttgg cggtctggat catgtattga gtttgacgga aaacgtcaac attctggtgc
     3001 tggatacgca atgctattcc aacaccggtg gtcaggcgtc gaaagcgaca ccgctgggtg
     3061 cagtaactaa gttcggcgag cacggcaaac gtaaagcgcg taaagatctt ggcgtcagta
     3121 tgatgatgta cggtcatgtt tatgtggcgc agatttctct cggcgcgcag ctgaaccaga
     3181 cggtgaaagc gattcaggaa gcggaagcgt atccgggacc atcgctgatc attgcttata
     3241 gcccgtgtga agagcatggt tacgatctgg ctctcagcca cgaccagatg cgccaactca
     3301 ctgctaccgg cttctggccg ctatatcgct ttgatccgcg tcgtgccgat gaaggcaaac
     3361 tgccgctggc gctggattca cgcccgccgt cagaagcact ggaagaaacg ttacttcacg
     3421 agcagcgttt ccgtcgtctg aattcgcagc agccagaagt ggcagaacag ttatggaaag
     3481 atgctgcagc taatttgcaa aaacgctatg acttcctagc acaaatggcc ggaaaagcgg
     3541 aaaaaagcaa caccgattaa atgctctgga taaggattat ccaattctaa aaaaaaagcc
     3601 cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc
     3661 caacagaaaa taaaatctgt gacaataaag gcatataacc cgcgcagaat aacgtatagg
     3721 aaattaaaag tataattttt attttgtatg tattatcttc gatacattca ccagacttat
     3781 tatcatttca gaggaattat ttgattaagg ttttacttaa ggcgtaacaa atgatattta
     3841 tcggctaact gaacttctcc tttaggatgt tttcactccc cgaaatgggg ataaaaggca
     3901 aataaaataa ctaaaggatt tattcaatga aaagaaaagt actggcactt ttaattcctg
     3961 ccctgctcgc cgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat
     4021 tagatctgta tggcaaagtt gatggcctgc attatttttc tgataattca gcgaaagatg
     4081 gcgaccagag ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactta
     4141 ctggctacgg tcaatgggaa tacaatattc aggcaaacaa cactgaatct tcaaaaaacc
     4201 agtcatggac ccgtctggca tttgccggac tgaaatttgc agattacggt tctttcgatt
     4261 acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat
     4321 ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgagcc aatggcgtcg
     4381 cgacttatcg taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt
     4441 atcaaggtaa caacgaagga gccagtaatg gtcaggaagg caccaataac gaacgcgatg
     4501 ttcgtcatga aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct
     4561 ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgaccaggtt aaccatactg
     4621 cggcgggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata
     4681 tttacctagc aaccatgtat tcagaaacgc gtaatatgac cccgtttggc gacagcgatt
     4741 atgcagttgc gaacaaaacc cagaactttg aagttactag taccagtttg atttttgtct
     4801 gcgtccggca gtctctttcc tgatgtctaa aggccgtgac cttcacgctg ctggtggtgc
     4861 agacaacccg gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac
     4921 ttactatttc aataaaaaca tgtccaccta tgttgactat aaaatcaacc tgttggatga
     4981 agatgacagt ttctacgctg ccaacggcat ctctaccgat gatattgtcg ctttaggtct
     5041 ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg ggctttttct gcttattctt
     5101 cctcctttga tctaaattaa aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc
     5161 gttacacttt atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct
     5221 atcgatattt cagattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca
     5281 aagattgatg atgcagaggt tcatttcctg acggtaatac cttcactgcc ctactatgcc
     5341 tctctgggtt tagcgtattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc
     5401 aaatcgcaac tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc
     5461 catgttgagg aaggctcgcc caaagaccgc attctggaat tggcgaagaa gatccccgct
     5521 catatgatca tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttccaac
     5581 gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc
     5641 ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta
     5701 atccgtacca tacacgccac agtttttata tcagatttct tatgctgagc gttccggcat
     5761 gacgcatact cttctgatgc catataacga attgagtcgc ttttaaatgt cgcaaaatca
     5821 agaaattagt aagaaagaac aatacaacct gaacaaatta caaaaacgtc tgcgtcgtaa
     5881 cgtgggcgaa gccattgctg acttcaatat gattgaagaa ggcgatcgca tcatggtttg
     5941 cctctccggg ggtaaagaca gctataccat gctggagatt ctgcgcaatt tgcagcaaag
     6001 cgcgccaatc aatttttcgc tggtggctgt taacctcgat caaaagcaac cgggcttccc
     6061 ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt gagtacaaga ttgttgaaga
     6121 gaacacttac ggtatcgtga aagagaagat tccagagggc aaaaccactt gctcactgtg
     6181 ttctcggcta cgtcgcggta tcctttatcg caccgcaacg gaactgggga cgacgaagat
     6241 cgcgttgggt caccatcgtg acgatatcct gcaaacgttg ttcttaaata tgttctacgg
     6301 cggcaagatg aaaggtatgc ctccgaaact gatgagcgat gatggcaaac atatcgttat
     6361 tcgtccactg gcctactgcc gcgagaaaga tattcagcga tttgccgatg caaaagcgtt
     6421 cccgattatt ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc
     6481 tgacatgttg cgtgactggg ataaacgtta tccagggcgt atcgagacga tgttcagcgc
     6541 gatgcagaat gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt tcaaaggcat
     6601 tacccacggt tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg aagagatccc
     6661 actacaaccg gcgggctggc agccagaaga agatgaaaat cagttggatg agttacggct
     6721 gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta tcgggctact
     6781 cttatggagg ccggataaga cgcggccagc gtcgcatctg gcaatcccga ataagatgtt
     6841 tactcttgca cccggcaatt caacatttca ttattttaat aaccgcaccc ggcacgtttt
     6901 tcctttaatc ttcccgccct gtaactgttt ccatgcttta tgagcaacag cctgacggac
     6961 cgcgacatag acatgcgccg gatgcacggc gattttgcca atatctgcgc catcaagccc
     7021 gatatctcct gtcagtgcac ctaatacatc acccgggcgc attttggctt ttttcccgcc
     7081 atcgatacac aacgttgcca tttctgcttc cagcgtcaca atggaactat tagctggcgg
     7141 cgtttgccag ttaagtttta tctgcaacat gtcagaaatg atattggccc gctgtgcttc
     7201 ttccggagca cagaaactga tcgccagacc gctatttcct gcacgagctg tacgaccgat
     7261 gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag ttcaccacca gctcaagcga
     7321 tttaatatcc agaccacgcg cagcaacatc agtcgcaacc agtacacggg cgctaccgtt
     7381 agcaaaacgt accagggtct gatcgcgttg ctccagatcg ccgtgtaacg acaatgcact
     7441 ttgctctact tcattcagcg cgtcgcagac agcctggcaa tcttttttgg tattgcaaaa
     7501 caccacacaa gaggatggct gatgcaagct taataaccgt tgcaacagag gaattttgcc
     7561 tttactggat gtctcataaa attgttgttc aatgggtggc aaagcatctg ttgagtcaat
     7621 ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt gcggcgatgg cttccggcca
     7681 ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca aaacggatga catcatcaat
     7741 ggcatcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca aagtattcag
     7801 cgcatccagt gataccgtgc ctttttgcaa gtgatccagc aaacgacccg gcgttgccac
     7861 gataatatgc ggcgcatgtt gcagcgaatc acgctgcata ccgaacggtt gaccaccgca
     7921 caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt cacctgccac
     7981 ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa atagcgacgc
     8041 atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc
     8101 ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct gcaccggcgt
     8161 catggttaaa taacccaact cattaaggtt cgtgagttgg gcgggaggca aaacattcag
     8221 ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtta gcaggcgcgt
     8281 atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc gggtggtggg
     8341 tcaggcatgg gttgcgggcg agggatcgga tcgggcactg gaacaggatc gccaggaatc
     8401 ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc cggtcattgg
     8461 gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg actcatcaaa
     8521 gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag taagacaata
     8581 tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagggagata ttgccctgaa
     8641 tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata cagacagccg
     8701 gaaaaattgc ttttgttaca accatttact acggtgcaac cataaagcaa caccaccaat
     8761 aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc cgccagggat
     8821 cccaccaagg ttgacgccaa ataacccggt cagaaaggta ctgggtaaaa agaccattgc
     8881 catcaacgac attgtatagg tacgacgagc taaattttcc tgcatcacct gagcgatttc
     8941 atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaaggc cgcgcccaag
     9001 gcgatcggca atatcctgca tccgacggcg ttggtcatcg ctcatccacg gcaaacgttc
     9061 actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca tcacaattaa
     9121 ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa ggagattatc
     9181 ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcactggaat gatcggtcaa
     9241 cgcatcgcac acatccacca gccatccccc gcaatcggtc ggacccgtgc cctcttccag
     9301 atcgctcacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa caattaaccg
     9361 cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg tgctgccgtt
     9421 tatacagcgc aatgtaatca gcgtgccttc accgaaacgg ctgactcggg gccgtgtgct
     9481 ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg ccagccattg
     9541 ggcgctatca tggtgtacat aattaaggtg gagccagcag ggatgcgctt catcaatcac
     9601 atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg caaatactgc
     9661 atccgggaca ttaacgtccg atcccttaat cgcttccact gtgcctccat catcaacgca
     9721 ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc cattaccctg
     9781 aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt ttccgtatac
     9841 aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat catatgacgt
     9901 ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taacgggatc tcaatgatta
     9961 cgcacaactt caataccctg gacttactca ccagtcctgt ctggattgtt tcgccctttg
    10021 aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt gatgcaagac ctcacgttta
    10081 gtcagctacg aaccggaccc tattccgtct cctcacaaaa agaactgccg aaatacctct
    10141 ccgatctgca aaaccaacac gatattatcg aaatcctcac tgttcagcgt aaagaagagg
    10201 aaacagcatt aagctgtcgg cttgttttgc gagagctgac agaaacagaa ccggtgatta
    10261 ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa agccagtcgc tcggcaaatt
    10321 atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct gcaccaatgt
    10381 tgttgattga cccgtcacga gatggacaaa tcgtcgatgc taacctcgcc gcgctcaatt
    10441 tctatggtta taaccatgaa acgatgtgcc agaaacatac ctaggaaata aatatgcttg
    10501 ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt acccggcggt cataaccctt
    10561 tgaattttgt tcataaactg gcggatggat ctactcgtca tgtgcagacc tatgccggac
    10621 cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt actgagcaaa
    10681 aacggctgga ggagcagctg gaacatgctg ctcaccatga cgcgatgacc ggattactga
    10741 atcggcgaca gttttatcac attacggaac caggccaaat gcagcatctc gccatcgctc
    10801 aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac gatctctatg
    10861 ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg ccccctcgaa agttgcgctc
    10921 gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg ctaccaagaa
    10981 ccccactgga taccgcgctt tcgctggctg aaactatccg cgtaagcgtg gcaaaagtga
    11041 gtatttcggg cttaccacgc tttaccgtca gcattggtgt ggcgcatcac gaaggaaatg
    11101 aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgggcg aaaaatgatg
    11161 gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc tcgagatcaa cgactgcgct
    11221 tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt tttacgtagt gcgacgtaac
    11281 acgccccgct gccgccatga tgcggtagcg cggtgcaata tgcctgaaca tcatcaaatt
    11341 cggtcagcca gcgcgccaca tagctgcgga caatattggc atgtgattta tcttcccgcc
    11401 ctttaccatg aataatcagc acattacgca aaccatccgc cagggcttgt tgaatgaaac
    11461 tgaacaccat tttgcggcac tcttccaccg gctggcgcaa aagattcagg ctcgcctgtt
    11521 gcggatattt accactgcgc agcttatcca gcaccccatg ctacaaccct tcctgccgaa
    11581 actccagcgg ctgacttagc gggatgatgt cgagaaatcc ggtagtgagg aaattatcaa
    11641 gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc cagtggacat
    11701 ctgtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc gatttgtcgt
    11761 caaggttcat gtaacctcct atgcaccttc acacgtcgtg attcagacgg gttaattgtt
    11821 catacttact gcatcgttcc cttcacagat tgaaacaata ctctacttca tacctgtgag
    11881 tatctgcgca ggcggggcat tttatactag cctgttagca gtatcgaaca gtttagcccg
    11941 agtttaattc agcacaggct ccgatcagga tgatttattc atagcaggaa ccaacaaaaa
    12001 tgcgataaat aacagaccac tgattagcag aaaggtaccg aaaatgccat agtttgcgcc
    12061 tccctcagca tcaattgact caataatttg gttgaagcca gtatttccga tgtctggtca
    12121 cttctactgc gattaaaaaa taaagcactt ctggaaatga agtcattcac cgtatgggga
    12181 acagaaacca ttttttcacc cattctcgaa aaatgaaaat tagttcgatt attgagaata
    12241 gagccacaca gggcaatacc aatcgcgccc ccaagattac gtgttaaatt aaatacacca
    12301 gaggctaact tcagccgttc ttttggaatt ccccccagcg ttaatgttac gatgggggcc
    12361 atcgcaaact gctgcgaaat ccctctaatt gcctgtggaa acagtaattc ctgccatccc
    12421 cattcgtggg ttataggggt aaaaagatac attgaaaaca caaatccgcc cagccccgcc
    12481 atcaacagcc atcgcagatt tattttttta cttaaccaga agtaaaaggg aacagaaaat
    12541 aactggaaaa tacctgttgt gcaaacggca aaacctatct cttcggcatt taatccgcga
    12601 atctgcccca aaaacacagg tattaaatat acagtagaga atatcccaac ccctccagaa
    12661 aaagaaaaaa aacatcccag agtgaaattt ttatccttaa aagcatgaag atccattatc
    12721 ggattggata ttgtaagtgt acgcgcagca aataaaataa aactcaccaa tgccagaact
    12781 gatgtcagca aaatagtatt atcatccagc catccccagc gcgctccttc ttcaagggta
    12841 tattccagac atcccagagt catggccaac aggataatac tcggataatc agcaactttc
    12901 agtaaagaga gatcaggttt atcaaaatta accagaaaag gaatactcaa aactaaatat
    12961 atgccaggta atatattgat ataaaatagc catcgccagt cgaggttatc agtgagccag
    13021 ccccccagag ttggccctag cgtcggtgac agtgaggcca gcgcactgac taccgcagca
    13081 gccaacccca actctttacc ctggtaataa ataaacgcca tcgtgaaaac taaagggatc
    13141 atggatgcgc cagcagctcc ctgtaaagca cgaaacaaaa tcatgatctg aatattccat
    13201 gcaagtccac acgcgatact cattaaagta aatattcccg cggatagagt aaaaagccaa
    13261 cgagtggaaa atacccgtga cagccaccca gagagcggaa taacaataat ctcagcaatt
    13321 aaatagctgg tctgaagcca ggccatttcg tctttacctg cggacagacc accacctatt
    13381 tcatttaggg atgacgatac aatttgaata tcaagctgag caataaataa tcccatacac
    13441 atactggcga aagcaatatg tttacgcata gaca
//
LOCUS       DAJTYL010000103        13296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYL010000103 DAJTYL010000000
VERSION     DAJTYL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.103"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..2636
                     /locus_tag="OO572_003055"
                     /pseudo
     CDS             1..2636
                     /locus_tag="OO572_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2646..6292
                     /locus_tag="OO572_003056"
                     /pseudo
     CDS             2646..6292
                     /locus_tag="OO572_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6353..6760
                     /locus_tag="OO572_003057"
     CDS             6353..6760
                     /locus_tag="OO572_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661970.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7302..8390
                     /locus_tag="OO572_003058"
     CDS             7302..8390
                     /locus_tag="OO572_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6661971.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8401..10680
                     /locus_tag="OO572_003059"
     CDS             8401..10680
                     /locus_tag="OO572_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661972.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10673..11809
                     /locus_tag="OO572_003060"
     CDS             10673..11809
                     /locus_tag="OO572_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6661973.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11806..>13296
                     /locus_tag="OO572_003061"
     CDS             11806..>13296
                     /locus_tag="OO572_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6661974.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISG"
BASE COUNT         3281 a         3515 c         3414 g         3086 t
ORIGIN      
        1 gccccgattt gctccctatg ccgcaagtga ctactgcgcc gatgtgttgc gtcatatcaa
       61 tcatccgttc gcattaacac tgcttatacg tgtagccggg catactaaac gctgtcacga
      121 tcgaatgacg aaagcctgcg ctgcgttccc acacgcagca ctggcagcac tggcggaact
      181 tctagtgcaa aaagaagaga atagttggcg cattatgcta atgacgatgt ttatctcgca
      241 accagcactg gcagaacagg tcattccctg gctttcgaca cccgcagttg cagtgctgaa
      301 atcatgccag caccaactga cacagccctc aaaccatgcc agcgccgatc tactgccagc
      361 catagtagtc tccccaccat ggatttcgaa aaagaaaaaa tcgccgattc cggtgctgga
      421 tttagcgcca ttaggcatcg agccaatctg ttatctgaca gaagaaatca gtaatcaact
      481 tttggcgaaa tatatctggt attcaaaaca catcacggtt agccatgaag aaagtactac
      541 caacctgttg gcaaggatgg gttttcaacg acggatcgct ggtacatata ttaaagctcc
      601 cgaagcggta gttgaggcat ggctaaatga agattattca accttactaa gtgaatttaa
      661 ggtgtttcat tcacctaccg ggcattattg gcagttgggg attttgacaa cattgccgct
      721 ggaggctgga gaaagcagta aaagcatgga atgcccttac tctatctcca cataccgata
      781 ccgaatacgc catgttacat tttggactca aaggtttacc tggtttagtt aactcaatta
      841 cacgctatcc acaggaagcc ttgcccatca cgaattactt cgcagcgagt gagctggctc
      901 ctgccgtcgc ccgtgccttc aacaaactca aaacgctacg cgaaaacgcc cgcacctggc
      961 tgttgaaata cccggaacat gcccttaccg gcctgctgcc tgcggcgctc ggcaaagccg
     1021 gtgaagcaca ggataacgcc cgcgctgcct tgcgtatgct taccgaaaac ggtcatcagc
     1081 cattactgca agaaatcgcc cggcgttata accagtcgga agtaacagac gcggtgaacg
     1141 ctctgcttgc gctcgatccc ttagataatc acccgacaaa aatccccact cttccgacct
     1201 tttatcagcc atcgatctgg acgcgcccgg tattaaaagc aaacgcccaa tcactgccag
     1261 ataacgccct cctccacctc ggcgaaatgc tccgcttccc tcaggaagag gctctgtatc
     1321 cgggattatt gcaggtgaaa gacgcctgta ccgctgactc tctggctgaa tttgcctggg
     1381 atctgtttac cgcctggcag accgctggcg cgccgtcgaa agagagttgg gcgttcactg
     1441 cgttaggcgt tctcggtaac gatgacaccg cccgcaaact gacgccatta atacgcgcct
     1501 ggcctggtga atcccagcat aaacgcgcca ccgttgggtt ggatattctc gccgctatcg
     1561 gtagtgatat cgccctgatg cagcttaacg gcattgctca gaaactgaaa ttcaaagcat
     1621 tacaggagcg ggcaaaagaa aagattgccg acattgccga gagccgcgaa ctcacggtag
     1681 cggagcttga agatcggtta gcaccggatc tcggtctgga tgataacggt tcgttgctgc
     1741 tggattttgg cccacgccag ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc
     1801 gtgatgtttc cggcagccgc ctgaaagacc tgccaaaacc aaacaaaagc gattatgagt
     1861 cacaggcgaa cgatgctgtt aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg
     1921 cggcccagca ggtagcaagg cttgaatccg ccatgtgcct gcgtcgccgc tggtcgccgg
     1981 aaaacttcca actcttcctg gttgtgcatc cgctggttcg ccacttaacc cgccgtctga
     2041 tttggggcgt ttatagcgcc gacaaccagc tactggcctg tttccgggta gcggaagata
     2101 acagctacag caccgctgac gatgatcttt tcaccctgcc ggaaggcgat atctctatcg
     2161 gcattcctca cgttctggaa atatcaccga cggatgctgt cgcctttggt cagctttttg
     2221 ccgactacga actgctacca ccgttccgtc agcttgaccg taacagttac gccctgacag
     2281 aagccgagcg caacgccagc gaactgaccc gctgggcagg cagaaaatgc ccgagcggcc
     2341 gggtcatggg gctggcaaat aaaggctgga taaagggcga accacaggat ggaggctgga
     2401 tgatcaaacc tctcggtcgc tggtcgttaa tcatggaaat cgacgaaggt tttgcggtgg
     2461 gtatatcacc agccgaactt agcgctgagc agctcttaag caagctgtgg ctatgggaag
     2521 gcaaagcgga aagctatggc tgggggagta attcaacaca ggaagcgcag ttctccgtac
     2581 tcgatgccat caccgccagc gagctaatta acgatattga agccctgttt gaataaggaa
     2641 agagcatgga caaggaatta ccgtggctgg cggataacgc ccaactggaa ctgaaatata
     2701 aaaaaggcaa aacaccactc agtcatcgca attggccggg cgaaccagtg cctgttatca
     2761 ctgaaagcat cattcagaca ctgggtgatg aattgctaca aaaagctgag aagaaaaaaa
     2821 acattgtctg gcgttatgaa aatttttcac cggagtggca gtccgccatt acgcaggcca
     2881 tcaacttgat cggcgaacac aaaccctcag tcccggcccg cacaatggcg gcactagcct
     2941 ttatcgcgca aaatgacaac caacagttgc tcgacgaaat cgtccaacaa gaggggctgg
     3001 aatatgcgac tgaggtggtg attgcacgcc agtttattgc acggtgttat gagagtgatc
     3061 ctctggtagt cacattgcag tatcagaacg aggactatgg ctatggttat cgctcagaaa
     3121 cctataacga attcgatctc cgactgcgta aacacctctc tctggcagag gaaagctgct
     3181 ggcagcgttg cgccgacaaa ctcattgccg cactgccagg aataaccaaa gttcgccgcc
     3241 cttttattgc gctgatcctc cccgaaaaac cagaaatcgc caatgagttg gtaagccttg
     3301 aatgcccgcg gactcatttt cattctaagg agtggttaaa agttgttgct actgacccca
     3361 aagcggtgag aaaactcgaa cgctactgga gccaggatat atttagcgat cgagaagcca
     3421 gctacatgtc gcatgaaaac cacttcggct acgcggcctg cgctgccctt ttgcgcgaac
     3481 aaggactggc agtcgttccg cgcctcgcga tatatgccca taaagaagat tgcggcagtc
     3541 tgctggtaca aattaaccat ccgcaagtca tccgcacctt gctgttagtt gctgataaaa
     3601 acaaacccag cctgcaacgt gtggctaaat acagtaaaaa cttcccccat gcgacactcg
     3661 ccgcactggc agaactgctg gcgttaaaag aaccaccagc ccgcccaggt tatccaatca
     3721 tcgaagacaa aaagctgcct gcacagcaaa aagctcgaga tgaatactgg cgtacgctgt
     3781 tacaaacgct gatggcatcg cagccacaac tggcagcaga agtgatgccg tggttaagta
     3841 ctcaagcccg ggcagtgctg aatagttatt tatcggcacc gcccaaacca gttattgata
     3901 gcaccgataa cagccaaatg cctgaaatcc tcgtttcacc accatggcgt gggagaaaga
     3961 aaaagacttc catacgtcac ttttcgctgc ctgatcttac gcttgcaccg caaacgtaca
     4021 ggtcgccgaa taaacactgg acgccggaac agcaaactgc aattgaccat tttagcgcca
     4081 tgtcttttga cgaacgtctt gctacgagag gagcggatat cttcttgcgg gaattaggtt
     4141 ttgacgttca tagcctggaa tttaataagc gtcttcttaa tggacagttg gatgcctttg
     4201 atgctcagca actgcaagaa tacacatcaa taggactatt gcgtacttat cacgcgcatc
     4261 aggccaaatc taaacagtac cgggaagacg ccaccaatgc attactggca caagatagca
     4321 ttgcgttgat gggagggtgg cgaacttttc agcacggatt ttactatggt aatgaacagc
     4381 gttgggggat ttggaatctg taccttatcg cgcagatgcc gcgcgaaatg gcggtatcgt
     4441 gctggcaaca aattgtcgag gctgattttc attacaccgg ggtggaatat ttgttaagtg
     4501 tgctgggtac cgatgctttg cctggattaa acaccgcctt tgcccgacat ccaaaagaga
     4561 tatttccgct gctgattcat ttcggcgcaa cagaactggc cctgcccatt gcccgcgtct
     4621 ggcacctttt tgcgggccag cgtaatctgg ctcgccagtg gattttacaa tggccggaac
     4681 atacggctac tgcacttatc cctcttgtct tcaccaaatc tagcgataaa agcgaagctg
     4741 cattacttgc cctgcgttta ctttacgaac atgggcatgg cgaatggcta caaactgtag
     4801 ccaatcgttg gcagcgtaca gatctatggc ctgccctgga acaattactt aaacagagtc
     4861 caatagagat ttacccggca cgcattccaa aagcccctga tttctggcat ccgcaaatgt
     4921 ggtccaggcc gcgccttatc actaataatc aacctgttac cgatgacgct ctggaaatta
     4981 tcggcgaaat gctgcgcttt acccaggggg gacgttttta tagcgggctg gaacaactga
     5041 aaacgttctg ccagccacaa acgctggcag cttttgcctg ggatctcttc actgcgtggc
     5101 aacaagctgg tgcccccgca aaagacaact gggcatttct ggcgttaagt ctctttggtg
     5161 acgaaagcac ggcacgggat ctaacgacac atatcctcgc ctggccacaa gaaggcaaat
     5221 ctgcccgtgc ggtcagtggc atgaacatcc ttaccctgat gaataatgat atggcgctga
     5281 tgcagctgca tcatatatcg caacgggcga aatcccgccc cttacgtgat aacgcggcgg
     5341 aatttcttca ggtagtcgca gaaaatcgcg ggctaagcca ggaagagtta gcggacagat
     5401 tagtcccaac actgggcctt aatgatccgc aggcgttgag ttttgatttt ggtccccggc
     5461 agtttaccgt tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc
     5521 gccagaaaag tgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac
     5581 tggcccgact aaaagggtta aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc
     5641 gtcttgaagc tgccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt
     5701 ttgttaatca tccctttacc cgtctggtta cccagcgatt aatatggggg gttatccggc
     5761 aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa
     5821 tgcgcaagat gagccaattg acctgcctgc ggacgctctg attggcattg cccacccgtt
     5881 agaaatgaca gcagaaatgc gcagtgaatt tgcacagctt tttgccgatt acgaaattat
     5941 gccgcctttt cgccagttgt cgcgccgcac ggtgctgctc acacctgacg agtcaaccag
     6001 taacagcctg actcgctggg aaggtaaatc cgctaccgtt gggcaactta tgggaatgcg
     6061 atacaaaggc tgggagtcag gttatgagga cgcatttgtc tatgacctgg gtgagtaccg
     6121 gctggtcctt aagttttcac ccggttttaa ccactacaat gttgatagca aagcgctaat
     6181 gagcttccgt tctcttcgag tgtaccgtga caataaatcc gtcacttttg ccgaacttga
     6241 tgtgtttgat ttgagtgagg cgttaagcgc acctgacgtc attttccatt aaaacaaagc
     6301 gggcagtgat gcgactgccc gttatctaca cgacttacca gcggggaaag cgatgatcgt
     6361 gcaaaaagag ctggttgcta tttacgatta tgaggtccct gtacctgaag atccgttttc
     6421 cttcagactt gagatccata aatgctctga attatttaca ggttccgtct accgactgga
     6481 acggttccgg ctacgtccaa catttcatca acgtgatcga gaggatgttg acccgctaat
     6541 aaatgatgcg ttgatttata taagagatga gtttattgat gagcaaaaat tacgaggcga
     6601 atcacctgaa cctgtaatag taatttttaa ttgtaaacta cagaatatat tcaaccaaca
     6661 aatagaatta tatactctag ataattcaag tcgcagcaca tgcaacttga agtatgacga
     6721 gtatagcttt attaaagggg ggaacggtat tcccccttag atgcgtttca atttgcccgt
     6781 tacttcaaca aatcaatcaa ccgtaacaga gccactaata gctccagtac agcaatgagc
     6841 acggttaact tttgtgtcag cgtgtccatc cgctctcctt attaaggagc acaacaccag
     6901 caccttaccc ttacactgcc tgtcgatact ggtttacgtt tgttgagtcg ccatgttgca
     6961 ctccggatca gagcactacc tgcggcacca cccgtccggt agccaggact tgaaataggt
     7021 tcgatccggc gcaacttccc aacagtcagg attataaatc tcattatctt aattgcaaca
     7081 ctgtccagag gtggttataa ttcgttcgtt ggaacacgat gttgcacagg cagtggtgtt
     7141 aggcctgaaa atagtaaata aaagaacctg cctcaccagc agtttttttt atttactgtg
     7201 atctgctttc cagatatttt tcgctcaaac aactaatgcg ccaaacattt attgcgcgta
     7261 aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg cagaataatc
     7321 atctgcagcg tccgcctgct gctgtgttat acgccgatga actggcaaaa ttaaaacaaa
     7381 atgataacgc accttgcccg cccggttggc agttaagttt gcctgcggcc cgtgctttta
     7441 tccttggcga tagcgcgcaa aatattagcc gtaaagtggt gattagtccc tccgctgtcg
     7501 aacgtatgtt agtcactctt gccaccggac gcggtttgat gttggtgggg gaacctggca
     7561 ccgcaaaatc tctcctgtct gaattactgg caacctctat tagcggcgat gccgggttaa
     7621 ccatccaggg cggcgcatct actaccgaag atcaaatcaa gtacggctgg aactacgccc
     7681 tgcttatcaa tcatggtcca tcaacggaag cgctggtccc tgccccgctt taccagggaa
     7741 tgcgcgacgg caaaatcgtc cgttttgaag agataacgcg tacaccgctg gaagtgcaag
     7801 actgtctgtt aggtatgctt tctgacaggg tgatgacggt gccggaactc actggtgaag
     7861 ccagtcagct ctatgcgcga gaaggcttta atattattgc cactgccaat acccgcgatc
     7921 gcggcgtcaa tgaaatgagt gctgcgctaa agcgtcgctt cgattttgaa actgtctttc
     7981 cgattatgga tttcgcccag gagctggagc ttgtcgccag tgcttcggcg cgtttgctgg
     8041 cccatagcgg tattccgcat aaagtgcctg acgcggtact ggagttactg gtccgcacct
     8101 tccgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat ggatacacta acagcgatta
     8161 tgtccaccgc tgaagccgtg aacgtggccc acgccgtagg cgtccgcgcc tggtttttag
     8221 cgaatcgcgc aggtgagcca gccgacctgg tggactgcat tgccggaacc atcgtcaaag
     8281 ataacgaaga agatcgcgct cgtctgcgcc gttacttcga acagcgtgtc gccacacata
     8341 aagaagctca ctggcaggct tattatcaag cccgccaccg cctgccgtga ggaaagatgc
     8401 atgagcgagc cgttaattgt cggcatccgg catcatagtc cggcctgcgc ccggctggtg
     8461 aaatcgttaa tcgaaagcca gcggccacga tacgtgttga ttgaaggccc tgctgatttt
     8521 aatgaccggg tagacgaact gtttttagcc caccagcttc cggtagctat ttacagttat
     8581 tgccagtatc aggacggtgc agcccccggg cgtggtgcct ggacgccatt tgctgaattt
     8641 tcgccggagt ggcaggcgct acaagccgat cgtcgtattc aggcacaaac ttacttcatc
     8701 gatttgcctt gctgggcgca gagtgaagaa gaggacgatt cgcctgatac gcaagaggaa
     8761 agccagacct tgctgctgcg tgccacccgc atggataaca gcgacaccct gtgggatcac
     8821 ttgttcgaag atgaaagcca gcaaactgca ttaccctctg cactggcgca ctattttgcc
     8881 caactgcggg gcgactcccc cggcgatgct ctaaatcgtc agcgcgaagc ctttatggcc
     8941 cgctggattg gatgggcgat gcagcaaaat aatggcgacg tgttagttgt ctgcggtggc
     9001 tggcacgctc cggcactggc aaaaatgtgg cgcgaatgtc cgcaggtaat taacacgcca
     9061 gaattgccgt cgctggcaga tgccgttaca ggttgttatc tcacccccta cagtgaaaag
     9121 cgccttgatg tgctggcagg atacctttca ggaatgcctg ccccggtctg gcaaaactgg
     9181 tgctggcagt ggggcttaca gcaggcggga gaacaactgc taaaaacggt tctcacccgt
     9241 ttgcgccagc acaagttgcc tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg
     9301 atggcactgg cacagttgcg cggtcataca ctaccgttac gcactgactg gctggatgcc
     9361 atagcaggat cgctgattaa agaagccctg aatgcgccgt tgccgtggag ctatcgcggc
     9421 gttattcatc ccgataccga tccgattctg ctaacgttga tagacacatt agcgggtgac
     9481 ggattcggta aacttgcccc ttctacgcca caaccgcctc tgccaaaaga tgtcacttgc
     9541 gaactggaac gtaccgcaat ctctcttccg gcggagctta ccttaaatcg ctttaacccc
     9601 aatggactag cgcaaagtca ggtgttacat cggctggcaa tactggagat cccagggatt
     9661 gtacgccagc agggaagcac actgacactt gcaggcaacg gtgaagaacg ctggaaatta
     9721 acccgcccgc ttagccagca tgcggcattg attgaggccg cctgctttgg tgccacactc
     9781 ctggaagccg cacgccataa attagaagcc gatatgctgg acgcgggtgg aatcggcagc
     9841 attaccacat gtcttagcca agcggcgtca gcgggtctgg cgtccttcag tcaacaatta
     9901 ctggagcaac tcacattatt aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg
     9961 ctggaagtgc tatatgcctt atggcggctg gatgaaatta gcggtatgca aggcgcgcag
    10021 atattacaaa cgacgttatg cgcggctatc gatcgcacgc tgtggctgtg tgaatccaac
    10081 ggcagaccgg atgaaaagga gtttcacgct cacttgcata gctggcaagc gttgtgtcat
    10141 atcctgcgcg atctacatag cggtgtgcaa ctccccggcg tttcgctttc tgcggcggta
    10201 gccttgctgg agcgacacag tcaggcgatt catgtcctgg cactggatcg cggcgcgact
    10261 cttggtgcgc tgatgcgtct ggaacatccc aacgctagtg ccgaagcggc gctgacgatg
    10321 ctggcgcagt tatccccggc acaatccggc gaggcgctgc acggtttgct ggcattagcc
    10381 cgtcatcaac tggcctgtca gccggcattt atcgccggtt tcagcagtca tttaaatcaa
    10441 ctaagtgatg ccgattttac caatgccctg cccgatttac gcgcggcaat ggcctggcta
    10501 ccgccacgag aacgcgggac gctggcgcat caggtgcttg agcattatca actggcgcaa
    10561 cttcccgttt cggcactgca aatgccgttg cattgtccac cgcaagccat tgcacatcat
    10621 caacaactcg aacagcaggc actggcatcg ctgcaacact ggggagtttt ccatgtctga
    10681 actgaacgat cttctgacca cccgtgagct acaacgctgg cgattaattc ttggcgaagc
    10741 ggcagaaacg acgctttgtg ggctggatga caacgcccgg cagatagatc acgcgctgga
    10801 gtggctgtat gggcgcgatc ctgaacggct ccagcgtggt gaacgctcta gtggattagg
    10861 tggctcaaat ctcaccactc ctgagtggat caacagtatt cacacgctgt ttccgcaaca
    10921 ggtgattgag cggctggaaa gcgatgccgt actgcgctac ggcattgaag atgtggtgac
    10981 aaatctcgac gtgctggaac gtatgcagcc ttccgaaagc ctgctacgcg ccgttttgca
    11041 caccaaacat ctgatgaacc ccgaagtact ggctgccgcc cgcaggattg tgcgccaggt
    11101 tgtggaagaa attatggcgc gactggcaaa ggaagttcgt caggcttttt ctggtgtccg
    11161 cgatcgtcgt cgccgctcat ttattccact ggcgcgaaac tttgatttca aaagtactct
    11221 gcgcgccaac ctgcaacact ggcacccgca acacggcaag ttgtatatcg aattcccccg
    11281 ctttaacagc cgcattaaac gccaaagcga gcaatggcaa ctggtcttac tggttgatca
    11341 aagcggatcg atggtcgatt cggtgatcca ctctgcggtg atggcggcct gtttatggca
    11401 gttacccggc attcgtaccc atctggtggc gtttgacaca agcgtcgttg atctcacggc
    11461 agacgttgcc gatccggtag agttattaat gaaagtgcaa ttaggcggcg gaaccaatat
    11521 cgccagtgcc atggagtatg gtcggcaact tattgaacaa cctgcaaaaa gcgtcattat
    11581 cctcgtgagc gatttttatg aagggggttc atcatcattg ctgacgcacc aagtgaaaaa
    11641 gtgtgtccag agcggcatca aagtgctggg actggcagcg ctcgatagca cagcaacacc
    11701 ttgctatgac cgcgatacgg cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat
    11761 gacaccgggc gagctggcat catggcttgc ggagaatctt cagtcatgaa ttcactacgt
    11821 ccggaattat tagaactgac accgcaggca ctgacggcgt taagcaatgc cggttttgtt
    11881 aagcgcagcc ttaaggaact ggaaaatggc aacgtcccgg aaatcagcca tgagaacagc
    11941 gctttaatcg ccaccttcag tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg
    12001 aaagaggctc agtgcaccta tggggccagc ggcatgtgcc gtcatcgcgt gatgctggtg
    12061 ttaagttatc aacgactttg tgctaccgcc cagcctacgg gaaaagaaga ggaatgggat
    12121 ccggcaatct ggctggaaga actggctacc cttcccgatg ccacccgcaa acgcgcacag
    12181 gcgctggtcg ctaaaagcat caccattgag ttgttctgtg cgccgggtga aattccctct
    12241 gcccgcttac cgatgagcga tgtgcgtttt tattcccgca gcagtattcg tttcgcccgt
    12301 tgtgattgta ttgaaggcac actttgcgaa catgtcgtac tggcagtaca ggcctttgtc
    12361 caggcaaaag cgcagcaagc ggaattgact catttaatct ggcagatgcg cagcgaacac
    12421 gtcacatcat ctgacgatcc gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt
    12481 caacaactaa gccaggcatt atggctgagc ggcatcagcc agccgcttat tcactacgag
    12541 gccgctttca gccgcgcgca gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa
    12601 tcgctacggc aactgcgcgc cagcgttgat gccttccacg cccgcgccag ccactatcat
    12661 gccggagaat gcttacgtca gcttgcggca ttaaacagtc gattaaattg cgcacaagag
    12721 atggcccgga gcgacagtgt tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc
    12781 tctggcattg ccggagaagc aaagcttgat catctgcggc tggtgtcttt aggtatgcgt
    12841 tgctggcagg atattgagca ttatggttta cgcatctggt ttaccgatcc cgacaccggc
    12901 agtattttgc acctttcgcg cagttggccg cgaagtgaac aggaagactc accggcagct
    12961 acgcgtcggc tgtttagttt tcaggctggc gcactggcgg gtgggcaaat tgtttcacaa
    13021 gcagcaaaac gcagtgccga tggcgagctg ctgttagcta cccgcaaccg cttaagcagc
    13081 gttgtaccgc tgtcgcctga tgcctggcaa atgttgagcg cgccgttacg ccagccgggc
    13141 attgtggctt tgcgggaata tttacgccag cgtccccccg cctgcatacg gcctcttaat
    13201 caggtcgata acttatttat tctgccggtc gctgagtgta tttcgctcgg ttgggatagc
    13261 agccgccaga cgctggatgc gcaggtaatc agcggc
//
LOCUS       DAJTYL010000104        12777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYL010000104 DAJTYL010000000
VERSION     DAJTYL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.104"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1449)
                     /locus_tag="OO572_003062"
     CDS             complement(<1..1449)
                     /locus_tag="OO572_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6661975.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1676..3094)
                     /locus_tag="OO572_003063"
     CDS             complement(1676..3094)
                     /locus_tag="OO572_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6661976.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3248..3592)
                     /locus_tag="OO572_003064"
     CDS             complement(3248..3592)
                     /locus_tag="OO572_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6661977.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3592..4518)
                     /gene="glsB"
                     /locus_tag="OO572_003065"
     CDS             complement(3592..4518)
                     /gene="glsB"
                     /locus_tag="OO572_003065"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6661978.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4582..5517)
                     /locus_tag="OO572_003066"
     CDS             complement(4582..5517)
                     /locus_tag="OO572_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6661979.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5597..6712
                     /gene="yneK"
                     /locus_tag="OO572_003067"
     CDS             5597..6712
                     /gene="yneK"
                     /locus_tag="OO572_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6661980.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6862..8052
                     /gene="ydeA"
                     /locus_tag="OO572_003068"
     CDS             6862..8052
                     /gene="ydeA"
                     /locus_tag="OO572_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6661981.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8077..8742)
                     /gene="marC"
                     /locus_tag="OO572_003069"
     CDS             complement(8077..8742)
                     /gene="marC"
                     /locus_tag="OO572_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6661982.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8954..9388
                     /gene="marR"
                     /locus_tag="OO572_003070"
     CDS             8954..9388
                     /gene="marR"
                     /locus_tag="OO572_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6661983.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9408..9791
                     /gene="marA"
                     /locus_tag="OO572_003071"
     CDS             9408..9791
                     /gene="marA"
                     /locus_tag="OO572_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6661984.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9823..10041
                     /gene="marB"
                     /locus_tag="OO572_003072"
     CDS             9823..10041
                     /gene="marB"
                     /locus_tag="OO572_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6661985.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10072..10971)
                     /gene="eamA"
                     /locus_tag="OO572_003073"
     CDS             complement(10072..10971)
                     /gene="eamA"
                     /locus_tag="OO572_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6661986.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11235..12353
                     /gene="ydeE"
                     /locus_tag="OO572_003074"
     CDS             11235..12353
                     /gene="ydeE"
                     /locus_tag="OO572_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6661987.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12480..12575
                     /gene="mgtS"
                     /locus_tag="OO572_003075"
     CDS             12480..12575
                     /gene="mgtS"
                     /locus_tag="OO572_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6661988.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3353 a         3278 c         2920 g         3226 t
ORIGIN      
        1 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
       61 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      121 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      181 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      241 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      301 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      361 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      421 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      481 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      541 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      601 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      661 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      721 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      781 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      841 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
      901 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
      961 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1021 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1081 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1141 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1201 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1261 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1321 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1381 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1441 tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt ggtgcgacag
     1501 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1561 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1621 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1681 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1741 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
     1801 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
     1861 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
     1921 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
     1981 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
     2041 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
     2101 gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt
     2161 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga
     2221 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
     2281 gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat
     2341 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
     2401 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
     2461 cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc atcatctgtt
     2521 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
     2581 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
     2641 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
     2701 agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc agacgcttat
     2761 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
     2821 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
     2881 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
     2941 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
     3001 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
     3061 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
     3121 tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt cgctaacagc
     3181 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
     3241 gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
     3301 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
     3361 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
     3421 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
     3481 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
     3541 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
     3601 cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc
     3661 tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac
     3721 aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc
     3781 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
     3841 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
     3901 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
     3961 tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
     4021 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
     4081 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa
     4141 catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa taccctgttc
     4201 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
     4261 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
     4321 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
     4381 acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
     4441 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
     4501 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
     4561 atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa
     4621 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
     4681 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
     4741 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
     4801 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc
     4861 aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
     4921 cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta acaaacgcgc
     4981 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
     5041 cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
     5101 tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc
     5161 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
     5221 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
     5281 ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     5341 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     5401 cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca tcacattcgg
     5461 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     5521 ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg atgacttatg
     5581 actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat ctcttcctga
     5641 tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg attttattga
     5701 tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca ccaactcagc
     5761 cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga cttcttttcg
     5821 gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc ctgtcaagaa
     5881 tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc ctatttattt
     5941 agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc caaagcctgt
     6001 cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt ttatgaatga
     6061 ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag cgcatacacc
     6121 acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac gccagtttga
     6181 tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc gattatggaa
     6241 taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca ccgccattct
     6301 cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg attcattagg
     6361 ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt tagcagaaca
     6421 agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat tgcactatca
     6481 ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag catcattaag
     6541 tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg taaaaaatga
     6601 aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct cccctgtcag
     6661 accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat
     6721 gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa cgcgatccag
     6781 atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg
     6841 caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta
     6901 cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt
     6961 ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg
     7021 ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt aatgaccagt
     7081 caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta
     7141 ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt
     7201 gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc tccggccggg
     7261 aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat ggtcttaggt
     7321 ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt cttcgcgatt
     7381 ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt actgcctagt
     7441 gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc
     7501 atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag
     7561 ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta
     7621 ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg
     7681 tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct
     7741 gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg
     7801 atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc taccgacgtc
     7861 gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc gttggtaggt
     7921 aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct
     7981 tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa
     8041 cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt taatgatttc
     8101 caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg
     8161 ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg agctgcgtag
     8221 gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac
     8281 ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat
     8341 ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat tggcactggg
     8401 ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg
     8461 cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc
     8521 gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg ccaccatcat
     8581 gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat
     8641 gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta acggcagcaa
     8701 caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt
     8761 gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag
     8821 ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca
     8881 attcattcat ttgacttata cttgcctggg caatattacc ccctacaact aattacttgc
     8941 caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg
     9001 cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct
     9061 ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac
     9121 tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga
     9181 tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata agcgcggcgt
     9241 actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc aattagttgg
     9301 ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg caacacttga
     9361 gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg tccagacgca
     9421 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
     9481 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
     9541 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
     9601 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
     9661 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc
     9721 ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca ttaaatcatt
     9781 acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc actttcatcc
     9841 gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca aaccacgcag
     9901 ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga acaaccaccg
     9961 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    10021 tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt attaacttcc
    10081 cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10141 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10201 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10261 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10321 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10381 atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10441 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10501 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10561 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10621 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10681 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    10741 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    10801 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    10861 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    10921 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    10981 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11041 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11101 tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct ggtagagcgt
    11161 cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt attttagaga
    11221 tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11281 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    11341 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11401 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    11461 ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11521 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11581 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11641 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    11701 aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    11761 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    11821 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    11881 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    11941 cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg ctgatgacag
    12001 caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc aacaacctgc
    12061 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12121 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12181 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12241 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12301 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    12361 cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa tatctttctc
    12421 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    12481 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    12541 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    12601 ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaccg
    12661 ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt tacgctaaca
    12721 ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt ctcaatg
//
LOCUS       DAJTYL010000105        12357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYL010000105 DAJTYL010000000
VERSION     DAJTYL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.105"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..64)
                     /locus_tag="OO572_003076"
     CDS             complement(<1..64)
                     /locus_tag="OO572_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6661989.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEV"
     gene            307..383
                     /locus_tag="OO572_003077"
     tRNA            307..383
                     /locus_tag="OO572_003077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Arg,seq:tct)
     gene            complement(429..1190)
                     /gene="envY"
                     /locus_tag="OO572_003078"
     CDS             complement(429..1190)
                     /gene="envY"
                     /locus_tag="OO572_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6661990.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1373..2263)
                     /locus_tag="OO572_003079"
     CDS             complement(1373..2263)
                     /locus_tag="OO572_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6661991.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2264..5232)
                     /gene="nfrA"
                     /locus_tag="OO572_003080"
                     /pseudo
     CDS             complement(2264..5232)
                     /gene="nfrA"
                     /locus_tag="OO572_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(5219..7456)
                     /gene="nrfB"
                     /locus_tag="OO572_003081"
     CDS             complement(5219..7456)
                     /gene="nrfB"
                     /locus_tag="OO572_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6661992.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7606..9048)
                     /gene="cusS"
                     /locus_tag="OO572_003082"
     CDS             complement(7606..9048)
                     /gene="cusS"
                     /locus_tag="OO572_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6661993.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(9038..9721)
                     /gene="cusR"
                     /locus_tag="OO572_003083"
     CDS             complement(9038..9721)
                     /gene="cusR"
                     /locus_tag="OO572_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6661994.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9878..11260
                     /gene="cusC"
                     /locus_tag="OO572_003084"
     CDS             9878..11260
                     /gene="cusC"
                     /locus_tag="OO572_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6661995.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            11284..11616
                     /gene="cusF"
                     /locus_tag="OO572_003085"
     CDS             11284..11616
                     /gene="cusF"
                     /locus_tag="OO572_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6661996.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11632..>12357
                     /locus_tag="OO572_003086"
     CDS             11632..>12357
                     /locus_tag="OO572_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6661997.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPTNVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKEEGRL"
BASE COUNT         2976 a         3158 c         3234 g         2989 t
ORIGIN      
        1 gaacttcaat agacattctg atgataggat gagtatccat aatgatcacc gacgttggtt
       61 tcatagttac cagtctcata ggagcggaca attttccgtt agggcggtaa attgtacttt
      121 gatacatgaa aatacgggtt ttcttgattc agacgcgcag cggtgtgcgt ttgtttgccg
      181 ctatagcgaa ataaatcaga aaatcagacg cggtcgttca cttgttcagc aaccagatca
      241 aaagccattg actcagcaag ggttgaccgt ataattcacg cgattacacc gcattgcggt
      301 atcaacgcgc ccttagctca gttggataga gcaacgacct tctaagtcgt gggccgcagg
      361 ttcgaatcct gcagggcgcg ccatttcttc ttcatttatg cccgtcttat ccgtttccgc
      421 tttgcccttc accacatcac tttttgtcgc tgtttggcga gataattcaa cggtgtcagg
      481 ctgtaaaacg ccttaaaaac agagataaag tacgacgtgc tgctatagcc acataattgc
      541 gccacctgag tgatattttt gttatccatc aataacatct gtacggcgta acgcatacga
      601 cactctgtga caatctggct atagctggta ttttcgtttt ttaatttctt tttgagcagg
      661 ctggggctta aacatagcga actggcgaca attcgcagat tccagtaatg ctgaatatcg
      721 ctttgaataa tgcggcagac gctgtcgcgg acgctgctgc gtaagatata catcagtagg
      781 gcaataaacc gcgattgctc aagaaagtta gacaatacgg taaaaagtaa tgcgcgcgtc
      841 aactccgctt cgcagggttt gctggattgc tgcttgctgt ggttggtggc tagccggaac
      901 acttccggtg tacggctatg acaagcgata accggggttg ctagccgcga ccagggcttt
      961 acacaggtta atttcttatt taaaaacaac agataatcgt tgatgatgtt acggttgatg
     1021 tgggtgatta atacgtgatt taacgttgag agttcaatga cgttattgtt gcacgcgaag
     1081 gcggccaggt agtttttcgg aagggtaaac gtagcatggt tattgacgct tacctctacc
     1141 tctttttcgg tcaggatcac cacgcaaggt tcactgctgc tcaattgcat ttcgcactcc
     1201 tcagatatca gaaactccgc tcaaaggatc tatgcttcct gcatgagtga tcggcccgtt
     1261 cgccgataac gatcttcttt ctttagcatg ctttttagca attaatcttg atggaattct
     1321 gacgagagcg aaagaggtaa gccaggtcgt acccgactta cctggagggg atttaatact
     1381 cgagaatgcc gtgcgcgacg ggcaaatagc gcagagagaa atagagcatg tctttaccgc
     1441 aagaggaacg tttcgctaac agcgaggcaa tttctgcgtc cggtttcggt ttcgctgtaa
     1501 aggttttgcc tttgccggcg acaaacagtt cataaacaat gccgcttgct ggggcggagc
     1561 tttggcaatc ggcgctggcc tgtagataac gttcacgctg ttcagcggta agtttatcca
     1621 cgccgctgcc atcctgtacc cagacattaa ctccggttgc tttaacttgt tccagcagtt
     1681 ggcgatagcc atcgggcgac atgtttccgg cgaaaaaact actgatataa accggttttg
     1741 ctgaaacatc gctaatcagc cgctgcgcgt tgtttaacca tgttagcaaa ggttgacggg
     1801 cggcttcgct gcgccagttc aggtcgtcaa tttccgcgct gatgtaccag ccatccggcg
     1861 ttatgccagg cgcggcgctc cataatctgg cttgctggag atcggcagcc agcaggcgat
     1921 taagatagct ttccagcgct gcagacgact gtttctggtg cataaaaaat tccggatcgg
     1981 cgttcagccc gacaatcagc ttcaggccag tctgttgcgc agctgcggca cgcttaaaca
     2041 ataacgtgcg ctgttctggc tgggtaaatg catcgccgta acgggtccat tgcaaaacca
     2101 gggtatcgaa gccttgaaaa cgtaactgac tcatcagccc ctgccactgg gtatcggtaa
     2161 cctgactatc tcggttttgt ggttgccaga taataccttt catcgcaaag gaaaaagggc
     2221 tgaccaaaag cagtgtcagc aatacgaaaa tgaacttacg catttaccag tgcactccaa
     2281 tggtgagaaa cgcgttgttg cgctctccgt tacgttgatt aatcgcctta aaggtatgtt
     2341 gatactcgac gccgagactg actttgtgcg gccaggcgtc gtagtgcgtc tcgccggtcc
     2401 aaatattcca gcggaccccg acaccgccaa gctgcgcgcc ctgagtgcct ttatcacgat
     2461 agccgttgtc ctgaacgtga gcgtaaggct caatagtctg tccgttagct accttctgat
     2521 gccagctgac gcgataatct gccgtccacg cctgaatatc ctggcggata tattgcgccg
     2581 catcgaggta caggttttgg gcaaaccagc ctgaaccgtt cgggtgccat tcgtcgctgt
     2641 atttgccgcc attaaagaat gaggcgctgg cgcgcagcat ggtatcggat gcgccatttt
     2701 gcccgttcag cggcaactgc tgttcgacgg cgaggaaaaa gatctgatcg cgcagcggct
     2761 tccagcgcag accggtgccg gacatcggat ttttcaccgg catcatcacc ccgttttctc
     2821 cggtatcggc aaagacgcgg ctataaactg agagcaggtc gccttccagc agcatattgc
     2881 gtccgatgcg gtactcggct tccagttgtc cgtagctacg atagcttttc ccaggcgctg
     2941 caccgccgac attattgtta gcggtactca ttgcgccgga acgcaagccg atggaagaat
     3001 cgaaactgaa cgtccagcgg cgaccgacct cctcatgcaa acggcggaaa ttgaagcgtt
     3061 gctgattttg ctctggggtc agtggggtta tgcctgatta tcaatgtcat caatcacgag
     3121 ccgggcgtag tgctgcgtcg caggcatgtc atccagacgc tggttcacgt aggccagttg
     3181 tcggatcagt gccggatcgt ccggaagccc tttatgcgcc tgttcgagca tttcccgcga
     3241 ctgtgcgata tcaccgctat cccacaaggc gtaaccaagc gctgcctggg tgttgctatt
     3301 atttggttcc agttccagcg cggcgcgcaa atcactcacc gcggccggaa cattatgacg
     3361 ttggcgataa attgtcgccc gcgcaacgta agagttggca gaaggggcaa tattgattga
     3421 gcgcgtgaga tcgttcagtg cgagttctgg ctgagctggg atataacgtc gcgcatgcag
     3481 ccaccagtat tgagcattgt ttcccagctc acgttgttct gcctgttgaa gccagcgatc
     3541 gcgagctgcg ccatttcccg ctgcctgagc ggtgttggcg gcggcgagca gatcatcatt
     3601 gctcatgtcg tgaagactga ttttctgcca ggccgccagt gcggtggcgt agtcctcaac
     3661 ctgatacgcc tgataggcta ccgcacgatg ttgccaggcg ctcggttgtc gttgttcggc
     3721 ctgaagccat gcatacaacg ccacaccggg tagcgtgtcc cgataacact ttgccagacg
     3781 gttccaggcg gcagcatcgt aggaaggcga catatcgccc agcaagcgaa ctattgccgg
     3841 gcaattatct gcaatacccg gcaactgact ttgccactga cgttgctccg ccagcggtaa
     3901 gggtttcgat aaaatcgcca ccttcgccgg cgttgctagg taaggatgac tttccagcag
     3961 agacgccagt cgcgccatta aagtctggct gacacgcgca tcgccctgga aaggatagcg
     4021 ttgcagcaat aaatcggcag cttcgcgtga ctgctcgttc tgcatcagtt gccaggtcag
     4081 ttgatccagg cggggaagat ttgtcggttc ttgctgatac agcaatcgtg ccagacgcag
     4141 agattcagcc ttgttacggg tcgccacgct gacagcataa cgctcctcaa gcatttcatt
     4201 ggcggggagg gtggcgagca gtttttgcgc tgcgtcgtac tgaccttctt ttaacagcac
     4261 cggtagcgtc gcgccaacaa catactggcg gttatcggca aactgcaccg tataattcgc
     4321 caatgcctga acggggttgg cgctgtattt agataacaga tagagccaac ttttctcttg
     4381 tgcgcccgtg gtaaacagcg gcttattttc aatgagataa tgctggaggc gtgctttttc
     4441 gccacgataa gccagcgcgg tcgcgtaagt aatatatgac tgaggatcgg tgaagatccc
     4501 ctgtgattgc agtgccagga tccgatcgtc cagctgcccg gcaagaagca cgtcaaacca
     4561 ctgacggtgt tctgccgcgc ttaatgtgtt ctgctggcgt gcttcattgt atagcgtatc
     4621 tgcctgggat cattgtttca ggtagattgc ccgttgcagc agatcggttc gcagcgtttt
     4681 tccttccggc gatgcagcaa acgtcgcatc gttcagttgc gctctggcga caggtaattg
     4741 tgccagccgc agggcattct gcccgacttc actgcgacaa cgcagggtcg gcgcagcgtc
     4801 gcccgctttt tgctgggcaa gcagttcttc aacagtcgta acgcttttca cttcaactgg
     4861 aatagccgcc agactgcgct caagtcgggc atctcctggg tgacgtttca gttgatcctc
     4921 aagcaacagc cgcgcccggt catcatgacc aaaatgacga taggcttccg caaggtataa
     4981 agtcagcgga atattatccg gcacctgttg gtgtatatat tcaaattcgc ggatggcggt
     5041 tgcttcgtcg ttatttttct gtgccttcag cgccttatcg agacggggat aaataacaaa
     5101 atggcgataa tcgctcagcc ccagctcttc tgcgctggtg ccgatattgt ctgcgagtgc
     5161 gctggtactc aataaagacg tcagcagtaa accagaccat ccgatgacgc gattaaggtt
     5221 attctccttc attttcggac tccagttgcg caacctgttc tgtgtttaaa cctgctttga
     5281 gtaatagtga ttgcatcgaa acttgtaatt cgcgttgaat tgtcaggacg cgatccaacg
     5341 tttcctggct gataacgcct tcggtgacca aaaacttgcc gagcggcaga gaactgcgtt
     5401 catggcgcaa taacaacacg ttaattgctg aacgattaat atgaccgagc gtggtcagta
     5461 tttcggcgaa caggaactga tgcggcacat attgccgcca gatttcaccg gtctgctgtt
     5521 ccgtgagcca ctgatgctga accgcattgt acaacattgc ccgcggatcg tgaccgcgtc
     5581 ggcgtgcata ccagtggcgt aaccctgtga caatttgtcc ccgcagaaca atgacgtaac
     5641 gcactttgcg tccgacttta cgcgtcaggg ccgccagcga aaccgggtca ataccatctt
     5701 cactgccgac aattaactca tcattttcca gacgcagcgg cagtactgca taatgcagcg
     5761 ctacggaggc tggcatttcg gcaatcagcg aggacgggat ctgccaggca tcgatggatt
     5821 cccacgccac gccgttttgc tctgccagcg cctgtgccag ctgctcggcg ctaatcagcc
     5881 cctgcatcag cattgaaccg cccaggcgta gaccttcgac gcgattacgc agtgctgtat
     5941 cgagttgttc ttcagtgatg acctgatttt ccagcagaat ttgacctaac gggcgcaacg
     6001 agcgggtatc gccagtgacg ctggggaagt catgcgttgt tttatcccac gcgacgcgac
     6061 gcggatcgcc gtgctgaaga acctgtttca gcacgcgcca gttagccata aagttaatca
     6121 ggttgcccca gaaaagacgc aggacggaaa gcaggccctg cgtgaggccg tagtagccag
     6181 tgacgaaaat cactcgctgc acgatgcggt taaccatcaa gccaaagttt agccacagca
     6241 gggtcattaa ccatgcgctg ccgctaaaaa tagaaaggaa atgccaggca tcgggccaca
     6301 aactttcata cgccagcaac agcaaaagct ggatcatcac cagcatcgcg aggaagctga
     6361 caaagttact gattgcccct ttgcggtcgc gccagagaaa gtagttcagc gtcaggctgg
     6421 aggtccattt atgggtttta aagccctgga aaacaatgcc gatgatccag cgagattttt
     6481 gtcgaaccgc agtcgaaaag gtatcgggga aatattcgcg cacgcagatc atgtttgacg
     6541 tccgtgcgtg ctgtaaaaat ttacgctgct cgcgttcttt ggcttcgtcc accaccggaa
     6601 aacggacaaa aatttccgtc ataccttttt ctttcaggcg gaagccaatg tcgtaatctt
     6661 cagtaagact ctgcacgtcg aaagcaatac cgttaccgtc agctaacagt gcggtcacgg
     6721 cgcggcggct gaaacaggtg ccgacgcctg cgctgggcac ttgtccggcg agggcttcac
     6781 gcaccggaac atctttgcca tgcagttctg aaaactcatc aatgtaagtc atgctggtga
     6841 agtgcgtcca ttcgcgttca aacggataca ctgggatctg aatcagatct ttacgctcga
     6901 ccagatagtt gaacagacgc aattccatcg gtgaaatcac atcttcggcg tcatgcagaa
     6961 taaaaccagc aaaagcgaaa ttggcgctac gttcaaactg ggtgatggcg tccagcacgt
     7021 tgttcaggca gtcggctttg ctggtgggac caggacgcgc gcagactacc ttatgcacgt
     7081 tcggaaagcg agcgcacact tcgtcaacat cacgttgagt atcggggtcg ttggggtagg
     7141 tgccaacaaa gatatgatag ttttcgtagt cgagcgtggt cgccgccagc tcggccatat
     7201 tgccgatgac gcccgtttca ttccacgccg gaaccataat cgctaacggt ttttcatctg
     7261 gtttatacag ttcgcggtaa ctcattcgcg ggtagcggcg ataaacactc aacttgcgtt
     7321 taatgcggcg tacccagtag acgacatcaa taaaaaaatc gtccagcccg ctgatgaaca
     7381 tgatgaccgc taacgttatc gcgattactt ttaagccgta gagccaggta gcaaaaacat
     7441 caagaagcca gtccacacaa aaaccttaca ttaacgctgg ttatgtttag ggtggcgtat
     7501 attaaggttt tttatgaatt gtgacagctt tttaccatta atatgtatga ctattgcggc
     7561 acgttatttt tacactggtt ataaaagttg ccgtttgctg aataattaag cgggtaatgt
     7621 gataacaaac cttgtccccc gcgcatctga cgttaccgca accgtgcctt tatgcgcgac
     7681 aacaatcgat ttcactatcg ccagcccaat accgctacct tcaccttttc gctggcggga
     7741 ggggtcaacg cgatagaaac ggtcaaacaa tcgcggtaag tgctcgggcg caatgggcgt
     7801 accggggttt tcgacggaaa cttgcacctg gtgatcgacc gtctgacagc gtactacaat
     7861 tgtctctctg gttggcgtat aacgaagggc attagaaagc aggttgctta gcgcccgacg
     7921 cagcatcaac ggatcgcccg caacctgacg tttgtcgcca acaaaccgca actccacgcc
     7981 gcgatcttcc gctagcgcct cgaaaaaatc gaacactttg ccgacttcat ccgccaggtt
     8041 gagcattttc ttttcgggga ttagctggtt gttatcggcc tgagcgagaa acagcatatc
     8101 gctgaccatt ttcgccattc gcgtcagctc ttcgagatta gagtagagca catcttccag
     8161 ctccttctgg ctgcgcgact ggctgagggc gatttccgtt tgcgtaatga gattagtaat
     8221 tggtgtgcga atttcgtggg cgatatccgc tgagaaattg gactggcgag taaagacatc
     8281 ctcaatacgc tcgatcatat ggttgaacga cagtaccagc tgttccagct caatcggcac
     8341 ggtctgcggg tcaaggcgaa cgtcgagatc tttcgaggtg atattctgga tttgacggct
     8401 gacgctgcgg atcggcgcgt gacctttatg taccgccaac agcacgataa agacgatcag
     8461 gatgctgatt accgatgcgg tcataataag tttattcatc aaatcattta tgtaatgaag
     8521 atgaaaatcg atcgacagcg cgatgtagag cgtataaatc ggtttgccgt ccaccaacgg
     8581 gccaaccggc aagttaatca tccgccagtt gctgtgttcc atatgcccgt gaccgtggcc
     8641 tggcatcata atcgtcgggc cggaaaggag atacacctcg ccaccccgcg cgtctttatc
     8701 gggtatggca tcacgtgcaa actcgcggat atcaggcgca ccgggggagt gatacaccgt
     8761 tttgccgtgg ctatctgcca gggaaatcaa cacgttggaa taaccactga cgatatcttc
     8821 cagcgtcatt aagcgtcggg cttgcgtttc gtcagggtga tttagtaccc gttcaagggt
     8881 ggcgctaatc tcttttaaat cattaatgtc ctgctcggca aaatgcactt ttactgagtg
     8941 gattatgatc catgcaaagg cgaaaaacgc cgcgatggtg gcctggctga taaaaaaggt
     9001 caggcgggtt gccagcgaaa acgggcgctg aaatggctta ctgaccatcc ggcacctcaa
     9061 gcatgtaacc cacgccgcgc acggtttgaa tcagcttcgg ctcaaagtcg ttgtcgattt
     9121 tgccgcgtag cagcttcacc gccacatcaa tggcgttagt gtcgctgtcg aaattcatgt
     9181 cccatacctg cgaggcgata agcgagcggg gcagcacttc gccctgatgg cggagaaaaa
     9241 actccagcag agtaaattct ttactggtca gagtgatgcg cgtgccgctg cgggtgactt
     9301 tgcggctgac gagatcgacc atcagatcgg caacctgaaa ctgactttcg ataatcaccg
     9361 ccgccccgcg ccgcaggaga gtgcgcaccc gcgccagcag ttcagcaaaa gcgaacggtt
     9421 tcaccaggta gtcatctgcc cccaactcca gccccttgac gcgatgttca atggtgccaa
     9481 gcgcggtaag caacagaatc ggcatccctt tattggcgga gcgcaacatg cgcacgatat
     9541 cccagccgtt cacgtctggc agcataatat cgaggattat caggtcataa tcaccggtca
     9601 tcgccagatg gtagccattc agcccgttgt cggccaaatc gaccacaaaa ccggcttcgg
     9661 ttaacccttt ggtcaagtat tctccggttt tcttttcatc ttcgacaatc aacagtttca
     9721 tatttcctcc gcatgttgcc cgggcaattc tagagtagcg ggatcagatg gcaatcgctt
     9781 attggcaaaa tgacaatttt gtcattttcc tgtcaccgga aaatcagagc ctggcgagta
     9841 aagttggcga cataaaatca ccagaaatta tgagcctatg tctccttgta aacttctgcc
     9901 attttgtgtg gcccttgcgc taactggttg ttcactggca ccggattatc agcgtccggt
     9961 aatgcccgta ccgcagcagt tctcactcag ccagaacggc ctggttaacg cagcggataa
    10021 ctaccagaac gtgggctggc gcaccttttt tgttgataat caggtgaaga cgctgattag
    10081 cgaggcgctg gtgaataacc gggatttgcg catggcgacg ctgaaagtgc aggaggcacg
    10141 ggcgcaatat cgtctgaccg atgccgaccg ctacccacag ctcaatggcg agggcagcgg
    10201 cagctggagc ggcaatctta aaggcgattc agccacgacg cgggagttct cgactggcct
    10261 taacgccagc tttgatctcg attttttcgg tcgcttaaag aacatgagcg aagccgagcg
    10321 ccaaaattat ttagccactg aggaagccca gcgcgcggtg catattttgc tggtttctaa
    10381 tgtcgcgcaa agctatttca atcagcagct ggcgtatgcg caattgcaaa tagccgaaga
    10441 aacgctgcgt aattatcagc agtcatatgc gtttgtcgaa aaacaactgt tgaccggtag
    10501 cagcaatgtt ctggcgctgg aacaggctcg cggggtgata gaaagtaccc gcagtgacat
    10561 cgctaaacgt cagggggaac tggcgcaggc gaataatgca ttgcaactgt tattgggaag
    10621 ctacggcaag ctaccgcaag cgcagacagt aaacagcggc agcctgcaaa gcgttaaatt
    10681 accggcgggc ttgtcgtcgc aaatcttatt gcagcgcccg gatattatgg aagctgaaca
    10741 cgcgttaatg gcggctaatg ctaatattgg tgctgcgcgt gcggcatttt tcccgtctat
    10801 cagcctgacc agcggaatat caaccgccag cagcgatctg tcgtcattat ttaacgctag
    10861 cagcgggatg tggaatttta ttcctaaaat tgagatcccc atttttaatg ccggacgcaa
    10921 ccaggccaat ctggatatcg ccgaaattcg ccagcagcag tcggtggtga attatgaaca
    10981 gaaaatccag aacgccttta aagaggtggc agatgcgctg gcattacgtc aaagcctgga
    11041 cgatcaaatc agcgcccagc agcgttatct ggcgtcgctg caaattaccc tgcaacgggc
    11101 gcgggcatta tatcagcacg gcgcggtaag ttatctggaa gtgctggatg ccgagcgttc
    11161 tttatttgca acccgacaaa ctttacttga cctgaattat gcccgtcagg ttaacgaaat
    11221 ttatttgtat accgccttag ggggcggttg gcagcaataa tttttaactc caggagagaa
    11281 taaatgaaaa aagcactgca agtcgcaatg ttcagtctgt ttaccgttat tggctttaat
    11341 gcccaggcta acgaacatca tcatgaaacc atgagcgaag cacaaccaca ggtcattagt
    11401 gccaccggtg tggtaaaagg tgttgatctg gaaagcaaaa aaatcaccat ccatcacgat
    11461 ccgattgctg ccgtcaactg gccggagatg accatgcgct ttaccatcac cccgcagacg
    11521 aaaatgagtg aaattaaaac cggcgacaaa gtggcgttta attttgtcca gcagggcaac
    11581 ctttctttat tacaggatat taaagtcagc cagtaaccca ggtttaatga gatgaaaaaa
    11641 atcgcgctta ttatcggcag catgatcgcg ggcggtatta tttcagcggc aggttttacc
    11701 tggtttgacc aggcggaacc gcacgcagaa aaaatgccga ccgcagaacg taaaatctta
    11761 ttctggtacg acccgatgta tcccaatacg cggttcgata aaccaggtaa atcgccgttt
    11821 atggatatgg atctggtgcc gaaatatgcc gatgaagaga gttctgcgtc tggtgtgcgc
    11881 attgacccga ctcagacgca gaatctgggg gtgaaaacgg ctaccgtcac gcgcgggccg
    11941 ctgacttttg cccagagttt cccgacgaat gtcagttaca acgagtatca gtatgccatt
    12001 gtgcaggctc gcgctgccgg gtttatcgac aaggtgtatc cgcttaccgt gggcgataaa
    12061 gtacaaaagg gcacaccgct tctcgacctg accattcccg actgggtgga agcgcagagt
    12121 gagtatttac tgttgcgcga aaccggcggt acggcgaccc agactgaagg cattcttgag
    12181 cggctgcgac tggcgggaat gccggaggcg gatattcgcc acctgatcgc cacgcaaaaa
    12241 atccagactc gctttacgct caaagcgccc attgatggcg tgatcaccgc gtttgatctg
    12301 cgcgcgggaa tgaatatcgc caaagataac gtggtcgcga aagaggaagg taggttg
//
LOCUS       DAJTYL010000106        12267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYL010000106 DAJTYL010000000
VERSION     DAJTYL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.106"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..98
                     /locus_tag="OO572_003087"
     CDS             <1..98
                     /locus_tag="OO572_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6661998.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="OO572_003088"
     CDS             95..496
                     /locus_tag="OO572_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6661999.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="OO572_003089"
     CDS             507..1247
                     /locus_tag="OO572_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6662000.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="OO572_003090"
     CDS             1306..1692
                     /locus_tag="OO572_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6662001.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="OO572_003091"
     CDS             1701..2030
                     /locus_tag="OO572_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6662002.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="OO572_003092"
     CDS             2002..5043
                     /locus_tag="OO572_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6662003.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="OO572_003093"
     CDS             5043..5372
                     /locus_tag="OO572_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662004.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="OO572_003094"
     CDS             5372..6070
                     /locus_tag="OO572_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6662005.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="OO572_003095"
     CDS             6075..6818
                     /locus_tag="OO572_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662006.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6755..7357
                     /locus_tag="OO572_003096"
     CDS             6755..7357
                     /locus_tag="OO572_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6662007.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="OO572_003097"
     CDS             7418..10897
                     /locus_tag="OO572_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6662008.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="OO572_003098"
     CDS             10965..11564
                     /locus_tag="OO572_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6662009.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12267
                     /locus_tag="OO572_003099"
     CDS             11628..>12267
                     /locus_tag="OO572_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6662010.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT         2890 a         2913 c         3927 g         2537 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc cgccgtggcg gtgtggctga gtgatgcgca
      241 gtacaccgga aaaatgacgg atgaggacga ctggcaggct gttctgcata ttgccgtttt
      301 cgtccgtgca caggcgccgg attctgagct ggatacgtgg atggagaaca ccatatttcc
      361 ggccatgaag gatattccgg tgctttccgg actcatcgac accatgatcc cactcggttt
      421 taactatcaa cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgagg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccgggggac gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcggtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tcagaaa
//
LOCUS       DAJTYL010000107        12238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYL010000107 DAJTYL010000000
VERSION     DAJTYL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.107"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            104..1801
                     /gene="maeA"
                     /locus_tag="OO572_003100"
     CDS             104..1801
                     /gene="maeA"
                     /locus_tag="OO572_003100"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6662011.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1935..2945
                     /gene="adhP"
                     /locus_tag="OO572_003101"
     CDS             1935..2945
                     /gene="adhP"
                     /locus_tag="OO572_003101"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6662012.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3091..3375
                     /locus_tag="OO572_003102"
     CDS             3091..3375
                     /locus_tag="OO572_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6662013.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3603..4256)
                     /gene="fdnI"
                     /locus_tag="OO572_003103"
     CDS             complement(3603..4256)
                     /gene="fdnI"
                     /locus_tag="OO572_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6662014.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4249..5133)
                     /gene="fdnH"
                     /locus_tag="OO572_003104"
     CDS             complement(4249..5133)
                     /gene="fdnH"
                     /locus_tag="OO572_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6662015.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5146..8193)
                     /gene="fdnG"
                     /locus_tag="OO572_003105"
     CDS             complement(5146..8193)
                     /gene="fdnG"
                     /locus_tag="OO572_003105"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7606..7608),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6662016.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8425..9306
                     /gene="yddG"
                     /locus_tag="OO572_003106"
     CDS             8425..9306
                     /gene="yddG"
                     /locus_tag="OO572_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6662017.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9561..10661
                     /gene="ompC"
                     /locus_tag="OO572_003107"
     CDS             9561..10661
                     /gene="ompC"
                     /locus_tag="OO572_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6662018.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10825..11310
                     /locus_tag="OO572_003108"
     CDS             10825..11310
                     /locus_tag="OO572_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6662019.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11420..>12238
                     /locus_tag="OO572_003109"
     CDS             11420..>12238
                     /locus_tag="OO572_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6662020.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKY"
BASE COUNT         3186 a         3045 c         2883 g         3124 t
ORIGIN      
        1 aatggtgttt ttatctgctt tatacttggg gacgacgccc tggcggtaaa gcaaagacga
       61 taaaagcgtg ccagggatgg atattcaaaa aagagtgagt gacatggaac caaaaacaaa
      121 aaaacagcgt tcgctttata tcccttacgc tggccctgta ctgctggaat ttccgttgtt
      181 gaataaaggc agtgccttca gcatggaaga acgccgtaac ttcaacctgc tggggttact
      241 gccggaagtg gtcgaaacca tcgaagaaca agcggaacga gcatggatcc agtatcaggg
      301 attcaaaacc gaaatcgaca aacacatcta cctgcgtaac atccaggaca ccaacgaaac
      361 cctcttctac cgtctggtaa acaatcatct tgatgagatg atgcctgtta tttatacccc
      421 aaccgtcggc gcagcctgtg agcgtttttc tgagatctac cgccgttcac gcggcgtgtt
      481 tatctcttac cagaaccgcc acaatatgga cgatattctg caaaacgtgc cgaaccataa
      541 tattaaagtg attgtggtga ctgacggtga acgtattctg gggcttggtg accagggcat
      601 cggcgggatg ggcattccga tcggtaaact gtcgctctat accgcctgtg gcggcatcag
      661 cccggcgtat acccttccgg tggtgctgga tgtcggaacg aacaaccaac agctgcttaa
      721 cgatccgctg tatatgggct ggcgtaatcc gcgtatcact gacgatgaat actatgaatt
      781 cgttgatgaa tttatccagg ctgtgaaaca acgctggccg gacgtgctgt tgcagtttga
      841 agactttgct caaaaaaatg cgatgccgtt acttaaccgc tatcgcaatg aaatttgttc
      901 ttttaacgat gacattcagg gcactgcggc ggtaacagtc ggcacactga tcgcagccag
      961 ccgcgcggca ggtggtcagt taagcgagaa aaaaatcgtc ttccttggcg caggttcagc
     1021 gggatgcggc attgccgaaa tgatcatcgc ccagacccag cgcgaaggat taagcgagga
     1081 agcagcgcgg cagaaagtct ttatggtcga tcgctttggc ctgctgacgg acaagatgcc
     1141 gaacctgctg cctttccaga ccaaactggt gcagaagcgc gaaaacctca gtgactggga
     1201 taccgacagc gatgtgttgt cactgctgga tgtggtgcgc aatgtaaaac cagatattct
     1261 gattggcgtc tcaggacaga ccgggctgtt tacggaagag atcatccgtg agatgcataa
     1321 acactgtccg cgtccgatcg tgatgccgct gtctaacccg acgtctcgcg tggaagccac
     1381 accgcaggac attatcgcct ggaccgaagg taacgcgctg gtcgccactg gcagcccgtt
     1441 taatcctgtg gtatggaaag ataaaatcta ccctatcgcc cagtgtaaca acgcctttat
     1501 tttcccgggc atcggcctgg gtgttattgc ttccggcgcg tcacgtatca ccgatgagat
     1561 gctgatgtcg gcaagtgaaa cgcttgctca gtattcgccg ctggtcctga acggcgaagg
     1621 tctggtacta ccggaactga aagatattca gaaagtctcc cgcgcaattg cgtttgcggt
     1681 tggcaaaatg gcgcagcagc aaggcgtggc ggtcaaaacg tctgctgaag ctttgcaaca
     1741 agccattgac gataatttct ggcaagccga ataccgcgac taccgccgta cctccatcta
     1801 agcctgcacc cggtagtgaa ggctaccggg ctatttccct ctcccttttt cagatctcat
     1861 ccatactggg tagtggcgaa taaatctcat ttgcctcacc tgctatgcag aacatcatcc
     1921 gaaaaggagg aactatgaag actgcagttg ttacgaagga tcatcatgtt gacgttacgg
     1981 ataaaacact gcgctcactg aaacatggcg aagccctgct gaaaatggag tgttgtggtg
     2041 tatgtcatac cgatcttcat gttaagaatg gcgattttgg tgacaaaacc ggcgtaattc
     2101 tgggccatga aggcatcggt gtggtggcag aagttggccc aggtgtcacc tcattaaaac
     2161 caggcgatcg tgccagcgtg gcgtggtttt acgaaggatg cggtcattgc gaatactgta
     2221 acagtggtaa cgaaacgctc tgccgttcag ttaaaaatgc cggatacagc gttgatggcg
     2281 ggatggagga agagtgcatc gtggtcgccg attacgcggt aaaagtgcca gatggtctgg
     2341 actcggcggc gcccagcagc attacctgtg cgggggtcac cacctacaaa gccgttaaac
     2401 tgtcaaaaat tcgtcccggt cagtggattg ctatctacgg tcttggcggt ctgggtaacc
     2461 tcgccctgca atacgcaaag aatgtcttta acgccaaagt gatcgccatt gatgtcaatg
     2521 atgagcagtt aaaactggca accgaaatgg atgcagattt agcgattaac tcacgcaccg
     2581 aagacgccgc caaaattgtg caggagaaaa ccggtggcgc tcacgctgcg gtggtaacag
     2641 cagtagctaa agctgcgttt aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg
     2701 tggctgtcgg tctgccgccg gagtctatga gcctggatat cccacgtctt gtgctggatg
     2761 gcattgaagt ggtcggttcg ctggtcggca cgcgccagga tctaaccgaa gccttccagt
     2821 ttgccgccga aggcaaagtg gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca
     2881 ccatctttac cgagatggaa gaaggcaaaa tccgtggccg tatggtgatt gatttccgtc
     2941 actaagaggt ctttgctgcg actcccatgt tcgggtcgca gcatcgcaca ctctccaaca
     3001 tgaaatggct aaatggatta ttgttgcgta ggaaggaaca ataatgatag tgttcccaga
     3061 gaagtaacgc tactgagagt aaagacacta atgaaaatgg ccaatcatcc ccgcccgggg
     3121 gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca
     3181 atggaaattg cgccctcaac ggcaagccga ttgctgaccg gaaaagcagc tttgacgcca
     3241 gaaatggcaa ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg
     3301 caaaacgcct ggagtctggc agaggcagaa aaaacggtgg ttgtgtcgcg attacgccgt
     3361 ctggttacgc aataaaaaat ggcaccctat aaagcgccat ttgaaactga tgacaaacgc
     3421 aacattgcct gatgcgctac gcttatcagg cctacacgtt cacttcaatc tattgtattt
     3481 gaagggtttt catgccggat aaggcattca cgacgcatcc gggatgaaca aagcccacgt
     3541 tgtctacaat atgaaaatgg cgccttttac agcgccattt tcagcttaaa gttcaaaaga
     3601 gattatatcc cttcttcact ctcttttttc gcttctgcct tctcgatttc acgataccag
     3661 cgcggatggt gtttcttcgc ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc
     3721 gaccctttca cccagaatgc catatacata tggatcagga tggcgtggat caggatgata
     3781 cccgcagccg cgtggatcag caggctgtag cgaacaacct gcatcgggaa gtactgcgca
     3841 aagtacggac gccagataat caccccggtc accagcagca cgaaaatcat gctcatgatc
     3901 gaccagaaca tcatcttttg cccggcgttg tacttaccaa catccgccac tttgtgctca
     3961 ttgcctttta atacttcgac aatgttcaac agccacggaa tatctttctt atccgggatg
     4021 ttgtgatgca caaaacgcac aaacataaac atcagtgcga cgaaaatcgc aatgccgaag
     4081 aaggggtgca aaatgcgtcc catctgcggc gtaccgaagg tttgcgtcag ccattgcagc
     4141 gtcgggaaga agaacgaaat cccggacagc gccaccagga agaagcaaat caccacggtc
     4201 cagtgacagg cgcgatcaat aaatttggtg cgcacaatca ttttcgactt actcatgatg
     4261 atcctcctcg tcatcgtcca cttccttatt cgggccaata ccgatgtagt ggaaaatcaa
     4321 cccggcaaaa gtggcaataa agccagccgc tgccagcggt ttcaacgcgc ctttccacag
     4381 gcttaccgag gtgtcgatct tcggatcttt cggcagaccg tgatacagct ccggctgatc
     4441 ggcgtgatgc agcacgtaca taacgtgcgt accaccgacc ccttccgggt tgtagacgcc
     4501 agcatgttcg taaccacgcg ctttcagttt cgccacgcgc tgttccgcca gctccagcat
     4561 ctccttcttg gtgccgaagt ggatagcccc ggtcggacag gttttcacac aagccggttc
     4621 ctggccgacg ctgacgcgat cgacgcagag cgtgcattta tatacccggt tatcctcttt
     4681 gttgaggcgc ggaatattaa acggacaccc ggcaatgcag taaccacagc cgatgcagtt
     4741 ttccgactgg aaatcgacaa tcccgttagc gtactgaatg attgcaccag cagacgggca
     4801 cgccttcagg cagcccggat cttcacagtg catacagccg tctttacgga tcagccactc
     4861 cagcttgcca ttctgttcgg tttcgctaaa gcgcatcacc gtccaggact tggcgctcag
     4921 atcggcgggg ttatcgtaaa ccccgacgca gtgccccact tcatcacgga tgtcgttcca
     4981 ctccgaacac gccacctgac aggctttaca gccgatacag gtggaaacgt cgataagttt
     5041 cgcgacttct gctttgtaat cacgcacctg agaaggcggc gtgatggagt tagttgcgga
     5101 ccttttgata atatcctgcg tttccatagc catctgttcg cccccttacg ccttctcgat
     5161 gttgactaag aacgctttat attccggcgt ttgcgagttt gcatcaccga cattcggcgt
     5221 cagagtgtta gcgatataac ctttacgcgc gacaccctca aagccccagt ggattggaat
     5281 acccaccgtt tcaacctgct gaccgtttac attcagcggt ttcagacggc gcgttaccac
     5341 tgccaccgcg cggataaagc ctcgcttgct ggagacagtg acacgatcgc cattattaat
     5401 gcctttcgcc gccgcctgcg tttcgctgat ttccacaaac tgttccggct gagcaattgc
     5461 gttgagcaac gcgtgcttgg tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc
     5521 cacatacggg aactgctctt ttttacccat ccgcagcgcg tcttgttcat acagacgaac
     5581 aaccgggtta gacaccacgt tcggatgcag cgggttagtg cccagcggcg tttcaatcgg
     5641 ctcgtagtgt tccgggaacg gaccttccgc cattttgttg atggcaaaca ggcgtcccat
     5701 cccttccggc tgcataataa acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc
     5761 cggaatatcg ttacccgtcc acttgctgcc gttccactgg atcagcatcc gtttcggatc
     5821 ccacggttta ccgttgatat ccgccgaagc acggttgtag agtacgcgac ggttgagcgg
     5881 ccacgcccag gcccatccca gcgtattccc cagaccggac gggtcggagt tatcgcggtt
     5941 tgccatctgg ttaccctgct ctgtccagct accggtgtag atccagcaag aagatgcggt
     6001 tgtaccgtca tcacgcagat gcgcaaagct actcagcaac tgacctttct tcgcaatcag
     6061 cacgccatta gcatcataga gatcttccaa cgcatagccg ttgttctctt tagctacttc
     6121 gtcagattgc ggttcgtgcg gctgcttgta gttccagctc atcttcatca gcggttctac
     6181 gcctttacca ccttcggcct ggtacaactc gcgcagatga tggtagatac ccgccagaat
     6241 ttcgccgtcg ttacgcgctt cgcccggcgc gtcctgacct ttccagtgcc actgcaacca
     6301 gcgaccggag ttagcgatag aaccatcctc ttcagcaaag caggtcgaag gcagacggaa
     6361 tacttcagtc tgaatagacg ccggatcgac atcgttcgac tcgccgtggt tctgccagaa
     6421 ggtagaggtt tcagtcacca gcggatcgat aaccaccatg tacttcagtt tgctcaggca
     6481 gctcaccact ttgtttttat ccgggaagga cgcaaccggg ttaaagccct ggcagaaata
     6541 accggtaact ttgccttcat ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc
     6601 ccacttcggc agccagtcat agccccagtt gttctctttc tgcgcggcat cgccatagaa
     6661 agatttcatc aggctaacga agaacttcgg atagttgctc cagtagttca cctgatcagc
     6721 cagcgtcgct ttcggcgtgt tcgcttccag atacgactgc aaatcaacct gtttttctga
     6781 cggcagcgtc agataacctg gcaggctggt agagagcaga cctaagtcag tcagcccctg
     6841 aatgttggag tgaccacgca atgcgttcac gccgccaccg gccataccca tgttaccgag
     6901 cagcgactgg atcatcgcca tagtacggat gttctgcgca cccacggtgt gctgcgtcca
     6961 gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg ctggtggagg ccagcacttc
     7021 acacactttc aggaagtcgg cttttggcgt accacagatg ttttcaacaa cgtccggcgt
     7081 gtagcgggaa acgtgcgctt tcagcaggtt ccacacacag cgcggatgag tcagtgtttc
     7141 atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc caggacgatt tgtcgtactg
     7201 gcgtttttca gcgtcataac cgctgaacag gccatcttcg aaagcaaaat catcacgcac
     7261 cagtaggctg gcgttggtgt aatgtttaac gtattcggcg ttgattttgt tgttttcgat
     7321 caggtagcgc aaaatgccag acaggaacgt aatgtccgta ccggaacgaa taggtgcgta
     7381 aatatccgcc acagaagcgg tacgcgtaaa acggggatcg acaacgatca aggttgcatc
     7441 gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt
     7501 accgcccatt accattacga cgttagcgtt tttgatatcc acccagtggt tggtcatagc
     7561 accgcgacca aatgttggag caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg
     7621 gttgtctacc gccagcatcc cgagggagcg ggcaaatttc tgggtcagca tcccggtttc
     7681 gttgctggca ccggaggcac acagcatacc ggtagaaagc caacggttta ccgttacgcc
     7741 ctgctcgttc ttttcaataa agttagcgtc acggtcagct ttcatcagct tcgcaatacg
     7801 ggagaatgct tcttcccagc taatgcgctg ccatttgtca gaacctggcg cacgatattt
     7861 cgggtagcgc agacggtttt cactgttgac gtaatccagc aaaccggccc ctttcgggca
     7921 cagcgcacca cggcttaccg gatgatccgg gtcaccttca atgtgataaa tcgcttctct
     7981 ggcgtttttt gcgccatcac ccaggctata catcaatagc ccgcaaccta cggaacagta
     8041 tgtgcaggtg ttacggatct ctttagcgcg taataatttg tagtttcgcg cctgagccag
     8101 tgcttgcttc ggggcaaagc ccaatgccgc taccgttgtt ccagccatac cgcccgcgca
     8161 gattttaaaa aattgtctgc gactgacgtc cattgctttc ctcttttttc aggggtatta
     8221 ctgcgcgagg aaactaacag taaaattctg aatctttttg cgcgaaatca aaaagaacac
     8281 gtgtttgcca caaaataatc cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa
     8341 cgcaccactg cctgacaggc cagttaaaaa aatgctataa aattcagctt aatttttaac
     8401 ggcaagagag acaaaacagc gagcatgaca cgacaaaaag caacgctcat agggctaata
     8461 gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagcga ggggctcggc
     8521 ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat cttcacggtt
     8581 ggatttccgc gtattcggca gattccgaaa ggctatttac tcgccgggag tctgttattc
     8641 gtcagctatg aaatctatct ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg
     8701 attgaagtgg gtatggtgaa ctatctgtgg cccagcctga caattctctt tgccattctg
     8761 tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc cctggtcggc
     8821 gtctgctggg tgttaggcgg tgagaatggg ttacattatg atgaaatcat caataatatc
     8881 accaccagcc cattgagtta tttcctggcg tttattggtg cgtttatctg ggcagcctat
     8941 tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta
     9001 acgggagcat gtctgtggat ttactatttt ctcacgccac aaccagaaat ggtatttagc
     9061 acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc ttatactgca
     9121 tggaatgtcg gtatattgca tggcaatgtc accataatgg cggtaggctc gtattttacg
     9181 cctgtacttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc
     9241 tggcaaggcg cactaatggt ctgcggcggt tccctgctct gctggctggc gacacgtcgt
     9301 ggttaaaata attgccgggt ctacggcccg gcagttaatc agataaattc cgacaatatt
     9361 aaaaaataaa tttcaaatac cagtaattca ccttatatct catttcgact tcgatcacat
     9421 atcgataaat aatattattc tatttttctc attgttttta atcaccattc tattttaaaa
     9481 acagtatgtc atttataaat gacgccgttt aaaattcgtt tagaaaatac gtttaacgat
     9541 aattaaaagg attctgttaa atgaaattaa aaatagttgc ggtggttgta actggtttgt
     9601 tagctgcgaa cgtagcacac gctgccgaag tctataacaa ggatggtaat aaactcgacc
     9661 tttatggcaa ggttaccgct ctacgttatt ttactgatga taagcgtgac gatggtgata
     9721 aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat caatgatcaa atgattggtt
     9781 ttggtcactg ggaatatgat tttaaaggct ataacgatga agccaacggc tcgcgcggca
     9841 acaagacccg tctggcctat gctggtttaa aaattagtga atttggctct ctggactatg
     9901 gtcgtaacta cggtgtcggc tacgacattg gttcatggac cgatatgttg ccagaatttg
     9961 gtggcgatac ctggagtcag aaagatgtct tcatgacata ccgtaccacc ggtctagcaa
    10021 cctatcgcaa ctacgatttc tttggcttaa ttgaagggct gaactttgcc gcgcaatatc
    10081 aaggcaaaaa taaacgtact gacaacagtc atctttatgg tgctgacgac acgcgtgcca
    10141 acggtgacgg tttcggtatc tcctcaactt atgtttatga tggctttggt atcggtgcgg
    10201 tatataccaa atccgatcgg acaaatgcgc aggaaagagc cgcagctaat cctctcaatg
    10261 cctccggtaa gaatgcagaa ctatgggcca caggtataaa gtatgatgcc aacaacatct
    10321 actttgcagc taattacgat gaaacattaa acatgaccac ctatggggat ggttatatct
    10381 ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca attcgacttc ggcttgcgcc
    10441 catcactcgc ttacctgaaa tcgaaaggca gagatctggg ccgctacgcc gaccaggaca
    10501 tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa caaaaatatg tcgacctatg
    10561 ttgattataa aatcaacctg attgatgaaa gcgactttac ccgtgccgta gatattcgca
    10621 ccgataacat cgtcgctacg ggcattacct atcagttcta acgtcctctc ctgaccagcc
    10681 cttctctggg ctggtttttg atttaagtgc tgctgtatga ctccttaagt aaaaatgagc
    10741 aattcttgat atttctggtc attcacctca attaatatta ttccatatcc tccggggaca
    10801 tcttactata atatttattg atgagtgatt atgaccaata ttaatacagc ttgtgtaaaa
    10861 aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat ctccagcaat
    10921 ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga agaaagagca
    10981 attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct tgatttgtca
    11041 tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt aaatctggaa
    11101 aacaattgtc taacttgtct tgacttagct gacaatgcca gtctcgttaa tatcaacctc
    11161 agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga aaacatttat
    11221 attaatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt ggttaatctg
    11281 gaagcgcaaa ataatagcct aaaaaaatga tatttctgat agttataaac tgaaatttct
    11341 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
    11401 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa
    11461 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
    11521 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
    11581 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
    11641 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
    11701 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca cctctatcaa
    11761 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
    11821 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat
    11881 tacacatata tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc
    11941 agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag aatcttgcga
    12001 atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg caggtaataa
    12061 aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac acaatgtctt
    12121 atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa ttaaaatatt
    12181 ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaatat
//
LOCUS       DAJTYL010000108        12231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYL010000108 DAJTYL010000000
VERSION     DAJTYL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.108"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            368..451
                     /locus_tag="OO572_003110"
     CDS             368..451
                     /locus_tag="OO572_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6662021.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(505..1857)
                     /gene="gorA"
                     /locus_tag="OO572_003111"
     CDS             complement(505..1857)
                     /gene="gorA"
                     /locus_tag="OO572_003111"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6662022.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(1929..2771)
                     /gene="rlmJ"
                     /locus_tag="OO572_003112"
     CDS             complement(1929..2771)
                     /gene="rlmJ"
                     /locus_tag="OO572_003112"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6662023.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            2974..5016
                     /gene="prlC"
                     /locus_tag="OO572_003113"
     CDS             2974..5016
                     /gene="prlC"
                     /locus_tag="OO572_003113"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6662024.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5024..5776
                     /gene="rsmJ"
                     /locus_tag="OO572_003114"
     CDS             5024..5776
                     /gene="rsmJ"
                     /locus_tag="OO572_003114"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6662025.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5825..7294)
                     /gene="dtpB"
                     /locus_tag="OO572_003115"
                     /pseudo
     CDS             complement(5825..7294)
                     /gene="dtpB"
                     /locus_tag="OO572_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7612..8046)
                     /gene="uspA"
                     /locus_tag="OO572_003116"
     CDS             complement(7612..8046)
                     /gene="uspA"
                     /locus_tag="OO572_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6662026.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8440..8775
                     /gene="uspB"
                     /locus_tag="OO572_003117"
     CDS             8440..8775
                     /gene="uspB"
                     /locus_tag="OO572_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6662027.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8846..10345)
                     /gene="pitA"
                     /locus_tag="OO572_003118"
     CDS             complement(8846..10345)
                     /gene="pitA"
                     /locus_tag="OO572_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6662028.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10577..11776
                     /locus_tag="OO572_003119"
     CDS             10577..11776
                     /locus_tag="OO572_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6662029.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3000 a         3098 c         3255 g         2878 t
ORIGIN      
        1 atccggacgt aaaaaaaccg acttcgcgtc ggttttttta ctcttcagcc cagagttggt
       61 ggctctggct ggagtgagaa tgccatcatc taacgcatca atgctaatac catactgaaa
      121 ctattgcaag gttatgctgg ttatttagcc taattacact gtatgattat ccagttagct
      181 ctgaggcatt ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac
      241 gggtggtgcc gttaactgat gctctggccg gagtgagatt gcccacatct aacaatgagg
      301 catgcgccgt gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag
      361 aaaacgggtg attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact
      421 gatccgcttt ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc
      481 acactactct tagcccttta acatttaacg catggtcacg aactcttctg ccgccgttgg
      541 gtgaatggcg acggtattgt cgaagtcttt tttggttgcg cccatcttca gcgccaccgc
      601 gaagccctgc aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc
      661 ttctggccca acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt
      721 atacatcgcg gtgaaagagg atttatacac tttcacctga tcgtcgccgt actgctcgcg
      781 cgcctgcggt tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg
      841 aatgttgctg taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg
      901 gcgacccgct gcaactgcca ccggtgtcag ttccactgca cccgtgttat cgcccaccgc
      961 gtaaatacct tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt
     1021 ggttttaacg ccagcggctt cgaggttgat gttgtcattg gcaggctcgc gaccaatcgc
     1081 ccaaatcagg caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc
     1141 atcggcattt ttcactaccg ctttcgggat ggcgttggta tgcaactgtg ggccttcggc
     1201 gttcatcact tcgaccagcg tttcagagat catcgggtcg aagctgcgca gcggcgcatg
     1261 tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta atcacgccag ccagctcaac
     1321 ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa
     1381 gccatcggaa tcaataccgt attccacgcc tggaatatcc gggtggctcg gacgaccgcc
     1441 ggtggcgatc agaatatgat cggccgtgat ggtttcgccg ttaacttcca gcgttttggc
     1501 atcaacgaag cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc
     1561 ataggaagta tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt
     1621 gaacttgttg atggtggtat cgaaaccata atcagggccg tacatatgga tcgcttcacg
     1681 gatttgcgcc gcgtgccaca tcactttttt cggcacgcag ccgacgttca cgcaggtgcc
     1741 gcccagctct ttggcttcaa tcagtgcgca tttctggccg tacatagccg cgcggttgat
     1801 ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac
     1861 atattgtcct tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat
     1921 ggctgcaatt actccggcac gatccagctt acggtggcgt gcccggtacc tgccggaacc
     1981 agtttgctgt gcagccacga cagcacgtta ttcatctgct gttccagctt ccacggcggg
     2041 ttaatcacaa tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagcaccgcc
     2101 agttcaattt gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat catgcgctta
     2161 atttgctggc gcagcaccac cggataccac aatgcgtaag tgccagtggc gaaacgtttg
     2221 taaccttctg ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg
     2281 tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt tggccttaag ttgctggaaa
     2341 ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa atccagaacg cagcaacggg
     2401 taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga
     2461 gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc tacggttgaa gtgttttacc
     2521 acattgatgt acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgata
     2581 ccttcgagat attcgccggt acgctcggca tgttcgctac ctaactgata acgcccggcc
     2641 cctgcgtggg tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc
     2701 aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga
     2761 taactgagca tgggtaaagg tgttccggta agtaaaaatt ggcccgtatt cggggcgcac
     2821 aaaagcctat caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct
     2881 cattgaaatt cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc
     2941 gcttccttaa cctctttaac caggactgcg ctaatgacga atccgttact gactcccttt
     3001 gaattgcctc cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggca
     3061 ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc gtacacctgg
     3121 gaaaatctct gccagccgtt ggcggaagtg gacgatgttt tggggcgtat cttctccccg
     3181 gtcagccacc tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc
     3241 ctgccgctgc tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg
     3301 taccgcgacc tgcgcgatgg cgatcattac gccacgctga acacggcgca gaagaaagcg
     3361 gttgataacg ccctgcgtga ttttgaactc tctggcatag gtctgccgaa agagaaacag
     3421 cagcgttacg gcgaaattgc tacccgtctt tctgaactgg gcaaccagta cagcaacaac
     3481 gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg acgaagcggg gctggcgggg
     3541 atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc
     3601 tacctgctga cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag
     3661 gctctgcgcg aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaat
     3721 gccggtaagt gggataacag caaggtgatg gaagagatcc tcgcgctgcg tcacgaactg
     3781 gcgcaactgc tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa
     3841 aacccgcagc aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc
     3901 gaaaaagagc tggagcaact gcgtgccttc gctaaagccg aatttggtgt cgatgagttg
     3961 cagccgtggg atatcgctta ctacagcgaa aaacagaaac agcacctcta cagcatcagc
     4021 gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct gtttgaagtg
     4081 gtgaaacgta tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg
     4141 gatgtacgtt tcttcgaact gtatgacgaa aataacgaac tgcgcggcag cttctacctc
     4201 gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga tggatggctg cgtaggccag
     4261 atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt atttgacttg taacttcaac
     4321 cgcccggtaa atggtaaacc ggcgctgttc actcacgacg aagtgatcac cctgttccac
     4381 gagttcggtc acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt
     4441 atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc
     4501 tgggagccgg aggcgctggc gtttatctct ggtcactatg aaaccggcga accgctgccg
     4561 aaagagttgc tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg
     4621 cgtcagctgg agttcggcct gtttgatttc cgccttcatg ccgagttccg cccggatcag
     4681 ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac tggttgccgt ggtgccatct
     4741 ccgtcctggg gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgcggca
     4801 ggttactaca gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag
     4861 gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt
     4921 ggcggttcag aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg
     4981 gatgcgatgc tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat
     5041 tgatgaaaca ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga
     5101 gcacgatgaa gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa
     5161 gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca
     5221 ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg
     5281 cgattatttg ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtact
     5341 ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat ccagtggttg ccgcgctact
     5401 cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg
     5461 gttgcagtta attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca
     5521 ggtggtctat ctcgatccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga
     5581 gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat gccgatggat tactggagcc
     5641 tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct
     5701 ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc
     5761 aggtacgcca gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga
     5821 ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca
     5881 tcactactgc aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg
     5941 gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc
     6001 cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca
     6061 aatgctgcgg caccagggca gcaatcatcg ccaggccaag ggcgctaata aacaattcac
     6121 ccaggctcta gaataaatac accagcacga taaaccatgg cgatgtcagc ccctggtcat
     6181 ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc cagtgagcac ataaacatgc
     6241 tgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa
     6301 tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga
     6361 ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca
     6421 gcgatgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa
     6481 aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga
     6541 tgatagtgac gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca
     6601 tcagccatgc acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc
     6661 tgaagctcat cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg
     6721 cgatgtaaac cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat
     6781 aaccgaatct atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga
     6841 tcgacatata gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact
     6901 tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca
     6961 gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg
     7021 ccagcacaag tgctccaaga acaatggtgc gtttggtccc caacaggtgg tcgccgacat
     7081 agccgccaat ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa gtgacaaaag
     7141 cctgctcttg cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc
     7201 cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt
     7261 gctgcagcat ccccatgggt gttgttgtat taatatttat taaccttcca ataccatcct
     7321 gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt
     7381 aatattgatg cggtgaatag attaatacac tattacaaca gaaaataacc agctgatcac
     7441 actaatgatg gaaaatatga tggtgttaaa ttttgtattg gcaggagatt ttgattgcgt
     7501 acgcagatcg taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa
     7561 agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggagggatg attattcttc
     7621 ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc
     7681 ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc
     7741 gtatttcttg attgcatcga ccagaacctg gcctaggtcg ccgctgccgc tcagggtttc
     7801 agtgattgga tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga
     7861 gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta
     7921 gtttacatct acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc
     7981 tttctctacc agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata
     8041 agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg
     8101 ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt
     8161 cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt
     8221 acccgttaga taaacaaatt ttcgacatga atattatatt gatagtggtt aaccttctgg
     8281 aaaaaaaaca acctgatctc ctacactatc tatagagccg ctcggatgtt ggtcaccaca
     8341 aaagcggttt ctccaggacc tggtgttaac tgattggcag cgtatcggga gcggtagccc
     8401 cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta tgataagcac cgtcgcatta
     8461 ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc atcactacgc
     8521 gcgttgttag tggtactgcg taactgcgat ccattgctct atcaatatgt tgatggaggg
     8581 ggctttttta cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg gtatatctat
     8641 gcccaacgtt atcgcgatca tcacgatgat gagtttattc gccgctgtga gcgggtgcgt
     8701 cggcagttta ttctgactag cgcattgtgt ggtctggtgg tggtcagcct gattgcattg
     8761 atgatttggc attaagcact tcagcaataa taacgtataa aaaaagcggg ccagtaaact
     8821 gacccgctcg ttttggtgcg tacgattaca ggaactgcaa ggagaaccag tacagcccgc
     8881 cggaaagtag taccgcagcc ggaagggtaa acacccaggc catcagaata ctggttacgg
     8941 ttttacgctg taagccgccg ccatctacca ccatcgtccc cgcgacagaa gaggagagta
     9001 cgtgagtggt ggaaaccggc atcccggtat aactcgccag gccgattgac actgccgccg
     9061 tcatctgggc agacatcccc tgagcgtagg tcatgccttt cttaccgatt ttctcaccga
     9121 tagtcgttgc cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg
     9181 ccatgatgat ccacaccggc gcatactcga tggtgctaag catgtcggac ttcagtttct
     9241 tcaacaggcg ctgatcgtca gcactcacac caggcatctt caccactttg tcgatagtgt
     9301 cagaaacgca cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt
     9361 agctttccac atcggtggtc aacatacctt tcaggcggtt gagcgcgtta atggtattcg
     9421 acggatggca gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact
     9481 gatcagcacc ggtagcctgt ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt
     9541 tgttgatggc atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga
     9601 agcctgctgg cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat
     9661 cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg atacgcgtcc
     9721 agaacggcgg ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac
     9781 gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa
     9841 acaccaggcc gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggga
     9901 tattgagtgc atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga
     9961 tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa accaaagtac caggtaccca
    10021 ggttccagat aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc
    10081 ccatattaag cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac
    10141 cacccagcaa aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac
    10201 gcatcgcgcg ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga
    10261 tggcttcgta gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat
    10321 ccaggccagc aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc
    10381 gcattatcag tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc
    10441 tcctgtatgg atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt
    10501 ttagtaatat tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt
    10561 ctaagaggag cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg
    10621 tatgttctgt tctgcgctgg caggtcaggt aggacgccgg gttctgctga tcgataatgg
    10681 taaaatacta gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct
    10741 ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc
    10801 acgttttacc cagtgggatt tcattgatct ggtcaataaa cacggtatcg cctggcacga
    10861 gaaaacgtta gggcaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt
    10921 ggatgagtgc gagaagggca atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc
    10981 gaaggatgaa acaggcttca cgcttgatct gaacggcatg actgtcggtt gcgaaaagct
    11041 ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa
    11101 gattgccgaa caatttggcc tcaacgtgct gccgacccgc gcgggtctgg tgccattcac
    11161 tctgcataaa ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt
    11221 gattaccgct gaaaacggca ccgttttccg tgagaactta ctcttcaccc accgcggctt
    11281 atctggaccg gcggtgttgc agatttcaag ctactggcaa ccgggtgaat ttgtcagtat
    11341 caatctgcta ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc
    11401 gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt
    11461 acagcaactc gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgt gtgaccaaca
    11521 ggcactgatt agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg
    11581 cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa ctttcttcac ggacgatgga
    11641 agcgcgcaaa gtgcctgggc tgtacttcat cggtgaagtg atggacgtta ccggctggct
    11701 ggggggctat aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc aggatttggc
    11761 gtcaattgtg aaataaccat gacgttttcc cgtcagccgt ttgtatatgt cgttattaag
    11821 tcattttggt acggttctgc atccgacatt ctggtgttaa ctcgctgttt tgttagcgaa
    11881 ttaacgctat tgccgttgtg gttattttat agtgtattta ttctcagaga atatatatta
    11941 ctgaaataaa aaacacaaag aataaccaaa ggaaattatt tgaaacgtaa aaggtatata
    12001 tttttaatct gtaaaattta aaatcacctc ctctggattg aatcaacttg ctctatttaa
    12061 gacgattgtt tttacatttt caccacctgt atcaggtggt gtttttttat tggcggcaat
    12121 gcctttatct tatttttatt acgttgtaaa tatggctatt cctaaatgaa atttgatttg
    12181 ctcagtttat ttgtgatgtc aggggagtgc ttgccatgag agtcgtgata t
//
LOCUS       DAJTYL010000109        12065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYL010000109 DAJTYL010000000
VERSION     DAJTYL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.109"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..314)
                     /locus_tag="OO572_003120"
     CDS             complement(<1..314)
                     /locus_tag="OO572_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR6662030.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVS"
     gene            602..1693
                     /gene="rutA"
                     /locus_tag="OO572_003121"
     CDS             602..1693
                     /gene="rutA"
                     /locus_tag="OO572_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6662031.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMEFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            1693..2385
                     /gene="rutB"
                     /locus_tag="OO572_003122"
     CDS             1693..2385
                     /gene="rutB"
                     /locus_tag="OO572_003122"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6662032.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2397..2783
                     /gene="rutC"
                     /locus_tag="OO572_003123"
     CDS             2397..2783
                     /gene="rutC"
                     /locus_tag="OO572_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6662033.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            2791..3591
                     /gene="rutD"
                     /locus_tag="OO572_003124"
     CDS             2791..3591
                     /gene="rutD"
                     /locus_tag="OO572_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6662034.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3601..4191
                     /locus_tag="OO572_003125"
     CDS             3601..4191
                     /locus_tag="OO572_003125"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6662035.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4202..4696
                     /gene="rutF"
                     /locus_tag="OO572_003126"
     CDS             4202..4696
                     /gene="rutF"
                     /locus_tag="OO572_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6662036.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            4717..6045
                     /gene="rutG"
                     /locus_tag="OO572_003127"
     CDS             4717..6045
                     /gene="rutG"
                     /locus_tag="OO572_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6662037.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6127..6234)
                     /locus_tag="OO572_003128"
     CDS             complement(6127..6234)
                     /locus_tag="OO572_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662038.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6607..7203
                     /gene="wrbA"
                     /locus_tag="OO572_003129"
     CDS             6607..7203
                     /gene="wrbA"
                     /locus_tag="OO572_003129"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6662039.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7224..7451
                     /locus_tag="OO572_003130"
     CDS             7224..7451
                     /locus_tag="OO572_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6662040.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7489..8730)
                     /gene="agp"
                     /locus_tag="OO572_003131"
     CDS             complement(7489..8730)
                     /gene="agp"
                     /locus_tag="OO572_003131"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6662041.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9023..10279)
                     /locus_tag="OO572_003132"
     CDS             complement(9023..10279)
                     /locus_tag="OO572_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6662042.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     EPFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10540..11460
                     /gene="cbpA"
                     /locus_tag="OO572_003133"
     CDS             10540..11460
                     /gene="cbpA"
                     /locus_tag="OO572_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6662043.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11460..11765
                     /gene="cbpM"
                     /locus_tag="OO572_003134"
     CDS             11460..11765
                     /gene="cbpM"
                     /locus_tag="OO572_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6662044.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11858..>12065)
                     /gene="torD"
                     /locus_tag="OO572_003135"
     CDS             complement(11858..>12065)
                     /gene="torD"
                     /locus_tag="OO572_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6662045.1"
                     /translation="LLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQY
                     DSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         2881 a         3058 c         3051 g         3075 t
ORIGIN      
        1 gagacttcca gcttcagacg gatgtactct tttatcgccg ccagcggggc gaaatcttca
       61 cgaaacgctt ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac
      121 agcgcctctt ttgacgggaa gtaatacagc agattggttt ttgaaacacc cgccaactct
      181 gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg
      241 ctaagaatcg cttttttctt cgcgcttact gcgcgcgaac gtttacccgt tgttttcact
      301 gcgccttgcg tcatgcgctc tcccctcttg tagattggta acaggatagc aaaacccccg
      361 cccggcctcg accttctgca ctctcatcgc gcacggatgc atgttttatg tgcaactgtt
      421 ttgaccgttt agtccacttt ttaccagata ttttagataa cacaatgcta acacattaat
      481 aatattcact ttttaaaaac tggcatccgc tttgcgaaca agccaatacg cggctgaaaa
      541 ggatatgcag gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat
      601 gatgaaaatt ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc
      661 gccgcagtac atgccgacct ttgaactgaa taaagccatt gcgcaaaaag cggagcatta
      721 ccatttcgat ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt
      781 ctgggatcac aaccttgagt cgttcacctt gatggcgggt ctggcagccg tgacctcgcg
      841 cattcagatt tacgccactg ctgccacctt aacgttacct ccggcaatcg tcgcccgtat
      901 ggccgcaacc atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg
      961 gcaaaagccc gagtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg
     1021 ttacgactat ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cggggaaaag
     1081 cgattttaaa ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc aaccgagtat
     1141 ccccatgaaa gtgatctgcg ccgggcaaag cgacgctggc atggagttct ccgcccagta
     1201 tgccgatttc aacttctgtt tcggcaaagg cgtaaataca cccacggctt tcgccccgac
     1261 cgctgcgcgg atgaaacagg ccacagagca aaccggacgc gacgttggct cttatgtgtt
     1321 gtttatggtg attgccgatg aaaccgacga tgccactcgc gccaaatggg aacactacaa
     1381 agcgggcgcg gatgaagagg cgttaagctg gctaaccgaa caaagtcaga aagatacccg
     1441 ctccggtact gacaccaacg ttcgtcagat ggccgatccc acttcggcgg taaacatcaa
     1501 tatggggacg ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag
     1561 cgtgcctggt gcagaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac
     1621 cttcggcgag cgcattcaac cactgatgca gtgccgcgcc catctctctg cgctgactca
     1681 ggaggtggca tgatgacaac cttaaccgct cgaccggaag ccattacctt cgatccgcag
     1741 caaagtgcgc agatcgtggt ggatatgcaa aacgcttatg ccacgccagg cggctactta
     1801 gatctcgccg ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgct
     1861 gtgaccgcag cgcgagcggc agggatgttg atcatctggt ttcaaaatgg ctgggatgaa
     1921 cagtatgtcg aggctggcgg ccccggctca ccgaattttc ataaatcgaa cgccctgaaa
     1981 accatgcgta agcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat
     2041 caactggtgg atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac
     2101 agcggtttct tcaatacgcc gctggacagc attttacgca gccgtggaat acgccatctg
     2161 gttttcaccg gtatcgctac caacgtctgc gtcgaatcga cgctacgcga tggctttttt
     2221 ctggagcatt tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca
     2281 cagaaagccg cgttgttcaa tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg
     2341 ttctgcgacg cgctttctcc cacgtccttt gctcgtatcg cttaaggagt ttagcgatgc
     2401 caaaatccgt aattattccc gctggcagca gcgcaccgct ggcccccttc gttcccggca
     2461 cgctggctga tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataataacg
     2521 tgctgtttgc cgatgaccca aaggcgcaaa cccgccacgt tctggaaact atccgcaagg
     2581 tgatcgagac ggcgggtggc acgatggcgg atgtgacctt caacagcatc tttattaccg
     2641 actggaaaaa ttacgccgcg attaacgaaa tctacgccga gttttttccg ggtgataaac
     2701 cggcgcgatt ctacattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca
     2761 caattgcgca tatcgccaag tgaggccgcg atgaaacttt cactctcatc tcccccttat
     2821 gctgatgcac ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta
     2881 ccgcaactgg cggtgctgga gcaggagtat caggtagtct gttacgacca gcgcggcacc
     2941 ggcaataatc ccgacacact ggcagaagat tacagtatcg cccagatggc agcggaactg
     3001 catcaggcgc tggtagccgc agggattgag cgttacgcgg tggtcggcca tgcgctcggt
     3061 gcgctggtgg ggatgcagct ggcgctggat tatcccgcgt cggtaactat gctggtcagc
     3121 gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacga
     3181 ttactgtata gcggcggcgc gcaagcatgg gtggaagcgc agccgttgtt cctctatccc
     3241 gccgactgga tggcggcccg cgcacctcgc ctggaggcag aagacgcgct ggcactggcg
     3301 cattttcagg gcaaaaataa tttactacgt cgacttaacg ccctcaaacg cgctgacttt
     3361 agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg
     3421 ctggtgccaa cagcatgttc cagtgaactt catgccgccc tgcccgatag ccagaaaatg
     3481 gtgatgccct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg
     3541 ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta aggaattgct
     3601 atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac
     3661 aacggctggc gggagacacc cgtcagcgat gagacgttac aggagcttta tgccctgatg
     3721 aaatgggggc cgacatcagc taactgttct ccggcacgga tcgtgtttat ccgcactgca
     3781 gaaggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa aaccctgacc
     3841 gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt accactactg
     3901 tttccccacg gtgatgcccg cagttggttt acctccagcc cacaacttgc cgaagaaaca
     3961 gcgtttcgca acagttccat gcaggcggct tatctgatcg tcgcttgccg tgcgctggga
     4021 ctggataccg gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc cttttttgcg
     4081 ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag cagcaaactt
     4141 tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac
     4201 tatgaacata gttgatcaac aaacctttcg cgatgcgatg tcctgtatgg gcgcggcggt
     4261 caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg
     4321 cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg
     4381 gccggtattc aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg ggcaggagcc
     4441 gctttcaaat ctttttggcg gcaaaacgcc catggaacac cgctttgccg ccgcccgctg
     4501 gcagacgggt gtgaccggat gcccgcaact ggaagaggct ctggtttcgt ttgactgccg
     4561 tatcagccag gtggtgagcg tcggcaccca cgacattctg ttttgcgcca tcgaagcgat
     4621 tcatcgtcac accacaccct acgggctggt gtggttcgat cgcagttatc acgcgctgat
     4681 gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg ttttcctcac
     4741 tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga acgattgccg
     4801 tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg
     4861 ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc ggggatcggc
     4921 acgttactgt ttttctttat taccggcggg cgcgtcccca gttatctcgg ctccagcgcg
     4981 gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat taacccgaat
     5041 atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt cattggcctg
     5101 gtggtgatga aaattggtac acgctggatt gaacgactaa tgccgccggt ggtgacgggt
     5161 gcggtggtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaag tgtatcggct
     5221 tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct ggtggcggtg
     5281 tttacccgtg gcatgatcca gcggttattg attctcgtag gattaattgt cgcctgtctg
     5341 ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc
     5401 agctatgctg cctggttcgg tctgccgcat ttttccactc ctggttttaa tggccaggcg
     5461 atgatgttga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag
     5521 gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc attcgtcggt
     5581 gatggtctgg caacgatgct ttccggctct gtcggcggta gcggcgtcac cacctatgct
     5641 gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt tgttgcagct
     5701 gccgtcatcg ctatgctgtt gggcttttcg ccgaaatttg gcgcgctgat ccataccatt
     5761 ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc
     5821 gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acgctaattt gattatggtc
     5881 gcagtaacct tcgtgctggg cgcgggtgat tttgctctca cgcttggcgg ttttacgttg
     5941 ggagggatcg gtacagcaac ctttggcgcg attttactca atgcgttatt aagcagaagg
     6001 ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga aaaccgccgg
     6061 gttaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgcattcg tcattgatta
     6121 gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac ctgcttctga
     6181 tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga
     6241 cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca
     6301 tattttcgct acgtgggact aagcttagtg aactgacgcg gacagacctg cgtttggtaa
     6361 tatcctgcaa cagcttctta gcgagaatag atatcatctc caataattat ccataagccg
     6421 ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt
     6481 gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac
     6541 gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg
     6601 ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg
     6661 gcacgcgcag tcgctgaggg tgcaagcaaa gtggatggcg cagaagttgt cgttaagcgt
     6721 gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca
     6781 ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc
     6841 cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg
     6901 gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc
     6961 ggtcaggaac aaaccatcac atccacctgg acgacccttg cgcatcacgg catggtgatt
     7021 gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg
     7081 ccgtacggcg caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa
     7141 ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc
     7201 taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg
     7261 aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg
     7321 ggtacgacga aaaaatggca gaaaagattt gggaagaagg tagtgatgaa gtgttagtca
     7381 aagcctttgc taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta
     7441 aaaacgttta accagcgact cccccgcttc tcgcggggga gttttctgtt atttcaccgc
     7501 ctcattcaac acgctatcaa acttatccat cgggcagaaa ccgttggcat ctatcgggca
     7561 accgcttaat tccagcgtca cgcgctgcgc aggtgcctgc agggttaacg catcggcatt
     7621 acgtaactgt tccgcactct ggtacacata ttcaattttc atcaaatcgc gattggcttt
     7681 gctgtcatgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc
     7741 atgcaactga tacggtttga aatccagcgc cgttaacaga gaggctatgt tggagtcgtg
     7801 cccaactaac accgtgattt tcggagcact ggcgcgatcg gtgaccaggg ctttgtcgat
     7861 ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acagactgtc
     7921 ctggtaaccg tttttcagct tcgacaatac cttccactgc tggtcagatt tgatttctcc
     7981 ccaggccacc tgatccatcg gaaaaccttc gtagtattgc aaagtaaacg catctaccag
     8041 cgagttgccg actttcagcg gcccggaaac acctggttct tgttgatact tggcgctaaa
     8101 ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt
     8161 atagttaacg attttttcca gtagctggta gctgtcggta agctggagtt tgctgagctc
     8221 tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcatcgg
     8281 gttaaaggtt gggtccatag tgcccatttt ttcctggtga tgcacaggaa tatcacaccc
     8341 agggaatgca ccagtaataa agaactgggc ggtagcgacg gtacgttgca gactattggc
     8401 ataggcataa acggtgtccg gcggcgggca ttcccccgat ttcactatcc cctgctctgc
     8461 cagccattca cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag
     8521 ttgcccaccg gggacgtccc attcgggcca tttattcggc gtcgactgct ccagcacact
     8581 gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca cttgctgtag
     8641 ctgatagcct tccggtacgg tttgtgcctg agcatttgaa gcgagtaaaa ctatccctgc
     8701 cacagttgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcatta
     8761 aagagtgtga cagaaatatg acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa
     8821 cgcctgtttt tctatataca tcttcaaaaa caggcaaggt ttgacgcaaa ctatgcctga
     8881 ttagcccctg cctcgcgtac gccgctgata attgcacctg tggctaaaca gaatatctgc
     8941 ggcgttacca atacagagac aatatttacc aataaaaaag tgtgagacta ataacaatgg
     9001 tttttagcat atgcctttgc cattaagttg cataacgatt taaatagtca agttgcgcat
     9061 tcatttcctt attttttaac ttataagcct gagaaaaagg ttcaggaggg aggtaaacat
     9121 cataatgatt gccagtgttt gttaaatatc catactcaag atcttcatct gttgaaaagg
     9181 attcatttcc caaggagatt gatgccacat tactattaac aataaataat gagcaaatgc
     9241 tatctgaaag aacagcaaga aaattcttca ccgctttaat cctatccgac gtgtcggtat
     9301 ctaaagcaag caaattcttg ttatcattaa atataaactc gggtaaatgc agtatgcatt
     9361 ttttgtcatt caagcaacta tcaatgaatt gttttttaaa aaacagaaag ctccatgaca
     9421 taaaactacc cacaccctga attttgttgt cagattgcag atcaacgcca aataatgtct
     9481 ttacatcttc cctgagatta tagaatgccg tatcaacaag agcctcgatc gtttgtttat
     9541 caacaatttt aataaactct tcgtatactt gcttttgcgt cttatggctg cccagcgccc
     9601 atgaatactc atgttctttt aaaaccagta tggctcttaa cagacaatat ccatcgccat
     9661 ctactggagc gtattttaat ccatatgttt tattaatttc actctgaata aaagatatgc
     9721 cagacaaatc aataccagta tctaaatcag atattcccgg catatttatc tgttctggct
     9781 ggatactcat attttcatta tctaaacaat actcagaaaa aacatcatca cacgcattaa
     9841 tgttttcaat atattccata aagccatacc aataataacc tttaaaatct gtgatagact
     9901 ctgaacctct gcccggcaag gacactctaa caatatcaac atcttgaaca tattgtatag
     9961 tatatgtaat acctcctgaa acccctgaat aaatttgtgt atcattagta cgcaatgttc
    10021 tcataaataa tgatagatca ttatcattct gcatttcctc aaattctttt tctagtgatt
    10081 cacattccag acatggagta tcctcttcat ttactttacg tctgagttca ttgtacaacc
    10141 gcataaaaca caccagtgtg ttttcaaaaa gcgtgattga atatgtttga tttttttcgt
    10201 actcattttt tatatcgttc caggcctgtt tcaaataatt atttgcagtg caactaattc
    10261 catgtatatt actacccata tatagcgtct ataaaattta ataaataatg acgccctagt
    10321 taaacttaaa gtgcctggtt caactatcaa aaatcgctca ccctttttca cctgtttaaa
    10381 atatgttcag caacccatct tgatggcgac ctcctctccg cgatgatttc aataacatat
    10441 tctgtgttgg catatgaaat tttgaggatt accctacact tataggagtt accttacagg
    10501 ggttccttca atttgtgttg atttacgcga gataacgcta tggaattaaa ggattattac
    10561 gccatcatgg gcgtgaaacc gacggacgat ctcaagacaa tcaagaccgc ctatcgtcga
    10621 ctggcccgta aataccatcc tgatgtcagc aaagaaccgg atgccgaagc ccgcttcaaa
    10681 gaggtcgctg aagcctggga agtgttaagt gatgaacaac gtcgcgctga gtatgatcag
    10741 atgtggcaac atcgcaacga tccgcaattt aaccgtcagt tccatcatgg cgacggtcag
    10801 agttttaatg ccgaagattt tgacgatatc ttctcgtcaa ttttcggtca gcatgcccgc
    10861 cagagccgtc aacgccccgc cgcacgtggc cacgatattg aaatcgaagt ggcggtattc
    10921 ctcgaagaaa cgcttactga gcataagcgt accatcagct ataacctgcc ggtttataac
    10981 gcctttggca tgatcgaaca ggaaatcccg aaaacgctga atgtgaagat cccggcaggc
    11041 gtcggcaatg gtcaacgcat ccgcctgaaa ggccagggga cgccgggcga aaacggcggt
    11101 ccaaatggcg atttgtggct ggtgattcat attgcgccac atccgctgtt cgatattgtc
    11161 ggccaggatc tggaaattgt ggtgccggtt agcccgtggg aagcggcgct gggtactaaa
    11221 gtcaccgttc caacactgaa agaaagcatt ttgctgacta tcccgccagg cagccaggcc
    11281 gggcaacgat tgcgcgttaa aggcaaaggt ctggtgagca aaaaacagac cggcgatctg
    11341 tatgcggtac tgaaaatcgt gatgccgccg aaaccggatg aaaacactgc cgcgctgtgg
    11401 cagcaactgg cagacgccca gtcgtctttt gatccacgta aagattgggg gaaagcataa
    11461 tggctaatgt tacggtgact tttactatta ccgaattttg cctgcatacc ggcatctctg
    11521 aagaggagtt gaatgaaatt gtcggtttgg gggtggttga accgcgtgag atccaggaaa
    11581 caacctgggt atttgacgac catgccgcca ttgtggtgca acgcgcggta cgcctgcgtc
    11641 atgaactggc tctggactgg ccggggatcg cggtggcgct gacgttaatg gatgatattg
    11701 cgcacctgaa gcaggaaaac cgcctgctac gccagcggct ttcccggttt gtagctcatc
    11761 cgtgatgtgt gtttgccgga tgtgatgacg catccggcaa ctgcgccata atgcctgatg
    11821 cgacgctaaa cgcgtctttt caggcgcata aacgacgtta tctgttttgg tggtcgcact
    11881 ccactaacac cagcaataac tggcttagtg ccgcgtaaaa accaaagctg tcatactgtc
    11941 gacaacgcgc agcaaactct ggtaaccatt gccgcagcgc cgtcagtgtt ttttgccgca
    12001 aactgtcgat tcttcgcgca ggaacggtcc cctctcccag cgaaaaatgc agataactca
    12061 acaac
//
LOCUS       DAJTYL010000110        12042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYL010000110 DAJTYL010000000
VERSION     DAJTYL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.110"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..460)
                     /locus_tag="OO572_003136"
     CDS             complement(<1..460)
                     /locus_tag="OO572_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6662046.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNI"
     gene            complement(491..1183)
                     /gene="fimC"
                     /locus_tag="OO572_003137"
     CDS             complement(491..1183)
                     /gene="fimC"
                     /locus_tag="OO572_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6662047.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1403..1945)
                     /gene="fimA"
                     /locus_tag="OO572_003138"
     CDS             complement(1403..1945)
                     /gene="fimA"
                     /locus_tag="OO572_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6662048.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2426..3292
                     /gene="folD"
                     /locus_tag="OO572_003139"
     CDS             2426..3292
                     /gene="folD"
                     /locus_tag="OO572_003139"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6662049.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3294..3506
                     /gene="ybcJ"
                     /locus_tag="OO572_003140"
     CDS             3294..3506
                     /gene="ybcJ"
                     /locus_tag="OO572_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6662050.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3614..4123
                     /locus_tag="OO572_003141"
     CDS             3614..4123
                     /locus_tag="OO572_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6662051.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4159..5544)
                     /gene="cysS"
                     /locus_tag="OO572_003142"
     CDS             complement(4159..5544)
                     /gene="cysS"
                     /locus_tag="OO572_003142"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6662052.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5718..6212
                     /gene="ppiB"
                     /locus_tag="OO572_003143"
     CDS             5718..6212
                     /gene="ppiB"
                     /locus_tag="OO572_003143"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6662053.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6215..6937
                     /gene="lpxH"
                     /locus_tag="OO572_003144"
     CDS             6215..6937
                     /gene="lpxH"
                     /locus_tag="OO572_003144"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6662054.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7055..7564
                     /gene="purE"
                     /locus_tag="OO572_003145"
     CDS             7055..7564
                     /gene="purE"
                     /locus_tag="OO572_003145"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6662055.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7561..8628
                     /gene="purK"
                     /locus_tag="OO572_003146"
     CDS             7561..8628
                     /gene="purK"
                     /locus_tag="OO572_003146"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6662056.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8765..9658)
                     /locus_tag="OO572_003147"
     CDS             complement(8765..9658)
                     /locus_tag="OO572_003147"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6662057.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9655..10302)
                     /locus_tag="OO572_003148"
                     /pseudo
     CDS             complement(9655..10302)
                     /locus_tag="OO572_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10305..11537
                     /gene="allC"
                     /locus_tag="OO572_003149"
     CDS             10305..11537
                     /gene="allC"
                     /locus_tag="OO572_003149"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6662058.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11548..>12042
                     /locus_tag="OO572_003150"
     CDS             11548..>12042
                     /locus_tag="OO572_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR6662059.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDV"
BASE COUNT         2921 a         3117 c         3078 g         2926 t
ORIGIN      
        1 tgatgttcag gcgcatcgct gcaaaatcaa agttaattgt ggcatcaggt acagctttca
       61 gtaaattgat gcatttattg ttttgctgct gggctaactc gggaaatgca ctgctattaa
      121 ggccaattcg ttcaaggagt acctggttaa aacagggcat taacccacct gatttatcac
      181 ctgtattttc tgttgtcgat tcaaatacga tatcctgtga accaatgaac tcatcattac
      241 gccagagatc cacccgatac acgcccgcag gttgatgatt acctctctca aagcgcgata
      301 aatcagcaac ggatgtgcca ttctctaata aaaatgccgg gttgaaataa ctttccgcat
      361 agcttaaaaa ggagggtaaa atggcaagag atgaataaca aattccggcc agacaccagg
      421 tatacctctg cggaatatcc gtagtagtgg gtattttcat aattagctct gatattgtca
      481 gatgattcgg ttagtttaag ttcacttctc tgaccggggt tactgaacca taatcattaa
      541 cggtctgaaa tttcagcttt ccctgcactc ctgagggtaa gggaatttgt gaactaactc
      601 tgggtgccgc catagcattc cccaactttt ggctgcccac ttgcaggtta acgagggtga
      661 cataaaaagg tgatggatta tgaacattga tatagttacc ggatcgcgaa aacttgagtg
      721 tgtccggcgc ttctgcgggt aattcttgta attgaattgg acggagaaat aatttcatgc
      781 gcgataaaat cgccagttgc aaaacattcc tgccgttcaa tgcattttta tctaccgaag
      841 ggatcgtctt aacattcatc cagaacagag actcacgatc tgccgccagc ggtggaccgg
      901 tgtaaataat acgcaaagta ttttcgcttt tgggttcact aacaaatagt ggcggtgtaa
      961 tgatgaatga cttttctttt acaccgctgc tgttttcaat ccacgaattg accagaaaga
     1021 gctcattggt atggctattt ttaatccata ccgcagtctg tttagcatca gcgggataaa
     1081 taatacgcgt ggcacctaat gcgatccctc cggcagcatg ggcgctggcc gaaatgatta
     1141 aataaaatat catgagcatc aataacttta ttttagtcat catagttaat acgccttgtg
     1201 tattcctgac ggcaaaaata aggagtataa agaaatgatt gctttggctt cttacaacac
     1261 atcattccct tttcatttaa taaagcggca tgatacaaag tgaacgtggt ttcgatttac
     1321 attgccacca taccgcttca gacaaccggt acattacttt gttataagac gtgatatata
     1381 attcaaaaca acgtggtttt gattattcgt atctcataat aaaagtcgcg tcagcatttg
     1441 cttgccctgc tgatgcactt gtaccggtgc ctttataacg tgcagaaaaa tgaagaacgt
     1501 tggtcccggg gatcaagttc tggttggttg agaagctatt cccatcaggt ttgagaattg
     1561 cggatgtatt atcaagtatt tcaataccga cacccgttgc tgttgttgta tttgtactgg
     1621 atacaatggc caataaatta tcattgattg catcagcctg accagaaaat gcaacggcag
     1681 cattagcggc aacaacagga tcgcagtcgt tcaactgaat ggtgaatgga attaatgcag
     1741 aggtattacc aacagcattg aaaatatcgg tacgatattg accgagtgtg acaacctggt
     1801 ctgccgagtc agtattcact gaacaggcag cattcaccag ttcgccttca aagtgaattg
     1861 taccgccatc tacaacggca gcataactac ccgtagcggc gaatagtgcg gcagccagcg
     1921 cagacgaaat aaatcttaat ttcatatata ttccttcaat ctcatttatc gactccacat
     1981 ccgtatataa ccgattactt tatttaagac actgatagta gtaaaattcc tttttatcct
     2041 ctaagaatgt cttaattgaa aatatgcact ctattctaaa aaatagagag ccccgttaga
     2101 tgaatacttc cgcgcaaaat atattcacca caaatataga cctgaagcgg taaattacca
     2161 ggctgaaaat tctttttata ttgtcaggta tttcttaaat tatcttaatc cttaaacaag
     2221 gaaataaatc agttccagat ttacaacgcc atcatgtacg aaaaatgaag ctttcaatct
     2281 cagcgacctc agcgacggtg cgcctaacct tcgcaagagg tcgcttcacg cgataaatct
     2341 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata gacgcatccc cttcgaccta
     2401 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
     2461 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggct cgtattgcag ccggactgcg
     2521 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
     2581 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
     2641 aaccaccagc gaagcggagc tgctggagct tatcgatgcg ctgaatgccg acaacaccat
     2701 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
     2761 acgtattcat ccggacaaag acgtggacgg tttccatcct tacaacgtcg gtcgtctgtg
     2821 ccagcgcgcg ccgcgtctgc gtccctgcac cccgcgcggt atcgtcacgc tgcttgagcg
     2881 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
     2941 tcgcccaatg agcatggaac tgttgctcgc aggttgcacc accaccgtga ctcaccgctt
     3001 cactaaaaat ctgcgtcatc acgtagaaaa tgccgatctg ttgatcgttg ccgttggcaa
     3061 gccaggcttt attcccggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
     3121 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
     3181 cgcctcatac attacgcctg ttcccggcgg cgttggcccg atgacggttg ccacgctgat
     3241 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     3301 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     3361 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagtcgacg
     3421 gtgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     3481 gacacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     3541 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     3601 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttccccttt gcattggttt
     3661 agggttgggt tcgaaaggtc tgttatttgc cggaatcatc ctggcgatgc tgcccgatgc
     3721 cgacgtattg tcgtttaaat ttggcgttgc ttacggcaat gtttttggtc atcgcggttt
     3781 tacccattcg ctggtgcttg cgtttgttgt cccgctctta tgtgtgctga ttggacgtcg
     3841 atggttcagg gctgggctga ttcgctgctg gctgttttta accgtctcgt tgctatcgca
     3901 cagcttactg gattcggtaa ccactggcgg taagggcgtt ggctggctgt ggccgtggtc
     3961 agatgaacgc tttttcgctc cctggcaggt gattaaagtc gcgccgtttg cattgtctcg
     4021 ttacaccaca gcgtacgggc atcaggtgat tatttccgaa ttgatgtggg tgtggttgcc
     4081 ggggatgcta ctgatgggaa tgttgtggtg gcgcagacga taaccggatg cgaaaactcg
     4141 catccggcaa ttgcgcaatt acttacgacg ccaggtggtc ccttgcgggc catcttccag
     4201 cacgatcccc atctcgttaa gacggtcacg tgccgcatct gccgccgccc agtctttcgc
     4261 tttacgggca tccagacgct gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc
     4321 tgcctgcgcg ccgctttgca ggaacgcttc cggttcttgc tccagcaggc ccaatacagc
     4381 ggaaagttta cgcaggtgag atgccattgc attcgccgct gccatatctt ctgctttcag
     4441 acggttcact tcacgcgcca tatcaaacag cacggaatag gcttccgggg tgttgaaatc
     4501 gtcgtccatc gcttcaataa agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt
     4561 tttatctgtg ccgcgcagcg cagtgtagag gcgctccagc gccgcacgcg cctgcttcag
     4621 gttctcttcg ctgtagttca gctggctgcg atagtggccc gacatcagga agtaacgcac
     4681 ggtttccgcg tcgtagtatt tcagcacatc gcgcacggta aagaagttac ccagcgattt
     4741 ggacatcttc tcgcggtcaa ccatcaccat tccggagtgc atccagtagt tcacatactg
     4801 accatcatgg gcacaggtgg actgcgcgat ttcgttctcg tggtgcggga acatcaggtc
     4861 tgaaccgccg ccgtggatat caaagtggtt acccagctgc ttgcagttca tggccgaaca
     4921 ttcaatgtgc cagccaggac gacccgcgcc ccacggagac ggccagctcg gttcgccctc
     4981 tttcgacatc ttccacagaa cgaagtccat ccggttgcgt ttgtcatcaa ccacgtcaac
     5041 gcgcgcgcca gcctgcagct ggtcgagatc ctgacgcgac agcacgccat aagttggatc
     5101 ggtcgggacg tcgaacatca cgtcgctgtt gtccgccaca taagcgtgac ctttggcgat
     5161 cagttgttca gtgagttcaa taatttctgc gatatggtgc gtcgcgcgcg gctccatatc
     5221 cgggcgcaga atgttcaaag catcaaaatc tttgtgcatt tcggcgatca tgcgatccac
     5281 cagcgccaca aagctttcgc cattttcatt ggcgcgtttg atgattttgt cgtcgatatc
     5341 ggtaatgttg cgcacatact tcagtttata gccgaggaaa cgcagatagc gcgcaaccac
     5401 gtcaaaagca acaaaggtac gcccgtgacc gatatgacag agatcgtaaa cggtgattcc
     5461 acacacgtac atgccgactt ccccggcgtg aataggctta aattcctctt tttggcgtgt
     5521 cagagtattg aagattttta gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt
     5581 tgcgtataat acccatattt cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt
     5641 gcggttatgc tataacacca ccctatatat gacccgaact gggttgaagc accaatcaaa
     5701 cggaacagga tgcaaaaatg gttactttcc acaccaatca cggcgatatt gtcatcaaaa
     5761 cttttgacga taaagcacct gaaacagtta aaaacttcct ggactactgc cgcgaaggtt
     5821 tttacaacaa caccattttc caccgtgtta tcaacggctt tatgattcag ggcggcggtt
     5881 ttgaaccggg catgaaacaa aaagccacca aagaaccgat caaaaacgaa gccaacaacg
     5941 gcctgaaaaa tacccgtggt acgctggcaa tggcacgtac tcaggctccg cactctgcaa
     6001 ctgcacagtt cttcatcaac gtggttgata acgacttcct gaacttctct ggcgaaagcc
     6061 tgcaaggttg gggctactgc gtgtttgctg aagtggttga cggcatggac gtggtagaca
     6121 aaatcaaagg tgttgcaacc ggtcgtaacg gtatgcacca ggacgtgcca aaagaagacg
     6181 ttatcattga aagcgtgacc gttagcgagt aatcgtggcg acactcttta ttgcagatct
     6241 tcatctctgc gtggaagaac cggcgatcac cgccggtttt ctgcgttttt tagcggggga
     6301 agcccgcaag gccgacgcgc tgtatattct tggcgatctg tttgaagcat ggattggcga
     6361 cgacgatccc aacccactcc atcgccagat ggcggcagcg atcaaagcgg tgtccgattc
     6421 cggcgttccc tgttatttca ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc
     6481 ccgtgaaagc ggcatgacat tattgccgga agaaaaggtg ctcgaacttt atggtcgccg
     6541 ggtgttgatt atgcatggcg acacgctgtg caccgatgac gcgggttatc aggcttttcg
     6601 cgccaaagtc cacaaaccct ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg
     6661 caaacgcatt gccgcgcgaa tgcgcgcgaa cagcaaagaa gccaacagca gtaaatcgct
     6721 ggcgatcatg gacgttaacc aaaacgctgt ggtcagtgcg atggaaaaac atcaggtgca
     6781 atggctaatc cacgggcata cccatcgccc ggcggtgcat gaacttatcg ccaaccagca
     6841 acctgctttt cgcgtggtac tgggtgcctg gcatacggaa ggttcgatgg tgaaagtcac
     6901 ggctgatgac gttgagctga ttcattttcc gttttaaaaa acccacaatt ttgctgattt
     6961 cacagccacg caaccgtttt ccttgctctc tttccgtgct attctctgtg ccctctaaag
     7021 ccgagagttg tgcaccacag gagttttaag acgcatgtct tcccgcaata atccggcgcg
     7081 tgtcgccatc gtgatggggt ccaaaagcga ctgggctacc atgcagttcg ccgtcgaaat
     7141 cttcgaaatc ctgaatgtcc cgcaccacgt tgaagtggtt tctgctcacc gcacccccga
     7201 taaactgttc agcttcgccg aaagcgccga agagaacggt tatcaggtga ttattgcggg
     7261 cgcaggcggc gcagcgcacc tgccaggcat gattgccgcc aaaacgctgg tgccggtgct
     7321 gggcgtgcca gtacagagcg ccgcactgag cggtgtcgat agcctctact ccatcgtaca
     7381 aatgccgcgc ggcattccgg tgggtacgct ggcgattggt aaagctggcg cggcaaacgc
     7441 ggcattactg gcagcacaaa ttctcgcgac tcacgataaa gagctacacc agcgtctgaa
     7501 tggctggcgc aaagcccaga ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc
     7561 atgaaacagg tttgcgtcct cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc
     7621 gaaccgttag gcattgctgt ctggccggtc gggctggacg ctgaaccggc ggcggtgcct
     7681 tttcaacaaa gcgtgattac cgctgagatc gaacgctggc cggaaaccgc attaacccgc
     7741 gagctggcgc gtcatccggc ctttgtgaac cgcgatgtgt tcccgattat tgctgaccgt
     7801 ctgactcaga aacagctttt cgataagctc cacctgccga ccgcgccgtg gcagttactt
     7861 gccgatcgca gcgagtggcc tgcggtgttt gatcgtttag gtgaactggc gattgttaag
     7921 cgtcgcactg gtggctatga cggtcgcggt caatggagtt tacgcgccaa tgaaaccgaa
     7981 cagttaccgg cagagtgtta cggtgaatgt attgtcgagc agggcattaa cttctctggt
     8041 gaagtatcgc tggttggcgc gcgcggcttt gatggcagca ccgtgtttta tccgctgacg
     8101 cataacctgc atcaggacgg tattttgcgc accagcgtcg cttttccgca ggccaacgca
     8161 cagcagcagg cgcaagccga agagatgctg tcggcgatta tgcaggagct gggctatgtg
     8221 ggcgtgatgg cgatggagtg ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggct
     8281 ccgcgtgtgc ataacagcgg tcactggaca caaaacggtg ccagcatcag ccagtttgag
     8341 ctgcatctgc gggcgattac cgatctgccg ttaccgcaac cagtagtgaa taatccgtcg
     8401 gtgatgatca atctgattgg tagcgatgtg aattatgact ggctgaaatt gccgctggtg
     8461 catctgcact ggtacgacaa agaagtccgt ccggggcgta aagtggggca tctgaatttg
     8521 accgacagcg acacatcgcg tctgactgcg acgctggaag ccttaatccc gctgctgccg
     8581 ccagagtatg ccagcggcgt gatttgggcg cagagtaagt tcagttaagc aggtgctcat
     8641 ttgattgccg gatgcggcgt aaacgcctta tccggcttac cgctccggta cccattgtag
     8701 gcctgataag acgcgtcaag cgtcgcatca ggcatggtgc accgattgcc ggatgctctc
     8761 atcactacag cgaaatacag gtccccgctt cgcccgccag cgtctcttca attcgcgata
     8821 acgccccaat ccacgcgggt ttactacggc ttctgacata accactcacc gccgttacct
     8881 tcggccccat cgaaccatcg gctttggcaa atggcgctaa ctcatccggt gtggcatggc
     8941 gaatggcacg ttgctgcggc gttccccagt tttcatatac cgcatcagca tcggtgagga
     9001 tcaccagtcc atcggcatta atctgctcgg cgagcaacgc agcggcgaga tctttatcaa
     9061 tcaccgcttc actccctgcc ccatcttccg tcacaggcac accgccgccg ccactgcaaa
     9121 tcaccacatg cccctctttg agcaacaact cgatggcttc gctgtcgaga attttacgcg
     9181 gttgcggaga cgccaccacc cggcgcaaat atttaccatc acgtttcatc tgccagccgt
     9241 aagccgcttc cagtgcctct tgttcttctg gctgataaac cggaccaata aatttctctg
     9301 gctgcaaaaa cgccggatca tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtaacag
     9361 gcggcatctg cggctgtgcg ctcaagctct gcgccagcat atagccaatc atcccctggc
     9421 tttccgccac cagtacatcc agcggatacg gttctacctc tttccacgcc agattctgta
     9481 acgccagcag ccccacctgc ggcccgttgc cgtgaacaat cgccagccga taagaacggg
     9541 ccaggcgtgc cagcgcgggt acagcactgg cgatattgcg atattgattt tccgccgtca
     9601 gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc tacaaccagt gttttcatgg
     9661 ttttccttgt aataattgtt ggccaagcca gaaccccagc agcgtatctg cccctgacgt
     9721 atgccccagc gccagcagcg aatcaatcgc aacagcggta cgtttcgggc aactcagagc
     9781 atggacaaag tgcaggagtg gcgaggcgaa atatccttgc gcggcataac gtaaataact
     9841 gacgctcacc gctgtggtaa cgagttgaag attgtcggaa caggcaaaaa acgggcgacc
     9901 ggcgcgcgca tctaaagcgc cataatacca ggccgccagc agcattccgc tcaacgtgtc
     9961 atcatgactt ggcgttaatc cggggccttt acccagccag tgccgccagt cggtcttaac
    10021 gccattgagc gcggcctgaa aacagtgacg aaactggcgt aattcagcgg gcagcggatc
    10081 gcttgccgcc atcgccagtg gcccgaaaag tccggtttcc tccgcgcgtt gcatccatgc
    10141 agctgcaagc ggttgaggat gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg
    10201 ctgtttaacc gtgaaacgcc cgaggcgaat cccttgagcc acaacctgtg gtcgttcatt
    10261 gccgcataat ccaccgcata acgcatcgaa ttgcgtgcgg cgaaattaca cattcccgtc
    10321 aagctataga agaaacgctg ccctggcttt cctcttttgg cgctgaccca acgggtggga
    10381 tgacccgttt actttattcg ccggaatggc tggaaaccca gcagcaattt aaaaaaagaa
    10441 tggcagcaag tgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga
    10501 gtggcaccga atatccacag gaagtggttc tgagcggttc gcatatcgat accgtggtta
    10561 acggcggtaa cctcgacggg caattcggcg cgctggcggc gtggctggca attgactggt
    10621 tgaaaacgca atacggcgca ccgttacgta cggtcgaagt ggtggcgatg gcagaagaag
    10681 aaggcagccg cttcccgtat gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc
    10741 ctgacgacgt gcggaatatc tgtgatgcca aaggaaatag ttttgccgat gcgatgaagg
    10801 cttgcggatt tactctgccg aacgccccac taactccgcg tcaggctata aaagcctttg
    10861 ttgaactgca tattgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg
    10921 tgaatgcaat tgtcgggcag cgtcgctata cggtgacgct gaacggcgaa tcaaaccatg
    10981 caggcaccac gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc
    11041 atcagtcgat cgaaaaagcg aaaaagatgg gcgatccgct ggttctgacc tttggcaaag
    11101 tagagccgcg cccgaatacg gtaaatgtgg tgccgggtaa aaccacgttc accattgatt
    11161 gtcgtcatac cgacgctgcc gtgctgcgcg atttcaccca acagttagaa aacgacatgc
    11221 gggcgatttg cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac
    11281 ccgtgccgat gaataaggag ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac
    11341 tgaattaccg ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg
    11401 taccaacctg catgattttt atccccagca tcaacgggat cagccataac ccggcggaac
    11461 gcaccaatat tactgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac
    11521 ttgcctggca ggaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc
    11581 gaagatttac tggctaaccg tgcgattgtt aaacacggta atttcgcact gttaaccccg
    11641 gatggtctga taaaaaatat tattccggac tttgaaaatt gtgacgcgac aatcctctcc
    11701 acgccaaagc tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggtggc
    11761 aaccaacagg gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat
    11821 atcactgcca aagccgaagg caaaacattt gccttaagcg aaggtggcta tctttattgc
    11881 ccaccaggct ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta
    11941 tataagcgcc gctatgttcc ggtagaaggc catgcaccgt ggctgttttc tggcaatgcc
    12001 agcgaactgg aacgcattca ctatgaaggt atggatgatg tg
//
LOCUS       DAJTYL010000111        11847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYL010000111 DAJTYL010000000
VERSION     DAJTYL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.111"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..765)
                     /gene="kdgT"
                     /locus_tag="OO572_003151"
                     /pseudo
     CDS             complement(1..765)
                     /gene="kdgT"
                     /locus_tag="OO572_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1025..1645)
                     /gene="sodA"
                     /locus_tag="OO572_003152"
     CDS             complement(1025..1645)
                     /gene="sodA"
                     /locus_tag="OO572_003152"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6662060.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1930..2964
                     /gene="rhaT"
                     /locus_tag="OO572_003153"
                     /pseudo
     CDS             1930..2964
                     /gene="rhaT"
                     /locus_tag="OO572_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2961..3827)
                     /gene="rhaR"
                     /locus_tag="OO572_003154"
     CDS             complement(2961..3827)
                     /gene="rhaR"
                     /locus_tag="OO572_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6662061.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3883..4719)
                     /gene="rhaS"
                     /locus_tag="OO572_003155"
     CDS             complement(3883..4719)
                     /gene="rhaS"
                     /locus_tag="OO572_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6662062.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5007..6476
                     /gene="rhaB"
                     /locus_tag="OO572_003156"
     CDS             5007..6476
                     /gene="rhaB"
                     /locus_tag="OO572_003156"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6662063.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6473..7732
                     /gene="rhaA"
                     /locus_tag="OO572_003157"
     CDS             6473..7732
                     /gene="rhaA"
                     /locus_tag="OO572_003157"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6662064.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7974..8798
                     /gene="rhaD"
                     /locus_tag="OO572_003158"
                     /pseudo
     CDS             7974..8798
                     /gene="rhaD"
                     /locus_tag="OO572_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8808..9122
                     /gene="rhaM"
                     /locus_tag="OO572_003159"
     CDS             8808..9122
                     /gene="rhaM"
                     /locus_tag="OO572_003159"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6662065.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9423..9869
                     /locus_tag="OO572_003160"
     CDS             9423..9869
                     /locus_tag="OO572_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6662066.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9880..11331
                     /locus_tag="OO572_003161"
     CDS             9880..11331
                     /locus_tag="OO572_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6662067.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11321..>11847
                     /locus_tag="OO572_003162"
     CDS             11321..>11847
                     /locus_tag="OO572_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6662068.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALD"
BASE COUNT         2825 a         3082 c         3118 g         2822 t
ORIGIN      
        1 cggcagtgcc atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca
       61 cgataattac tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca
      121 aatcaatggt attgcccagc gcgaaggcaa agaatggaat cagcgtttgc accgctttgc
      181 tgaaaaattc tcgtaattca gggtcaagat tccccagggc aaagcccacc aggaacggta
      241 atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat cccggcagtg cccagaataa
      301 tcatcgtcat gagcggcccg gactccaacg acatcaacac aaatgcccag cttcttcttt
      361 cgtaccgtac tgctgcatga tggaagcgta aagtccgccg ttggtcatat ccatcgccgc
      421 caccagcgcc agcgttgaaa gtccggcaaa gaatccaact tcaacaccat gttccggaat
      481 aatgcgcgag gcaattgccg caaccaccca cgcgacggca attttagtta ccaccagcgt
      541 accggattta cgcagtaccg ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa
      601 aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc ccgttggtga atgatccaaa
      661 atatttcccc gcccccggcg agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag
      721 catcatcccc cccgggattt tctcaatcga gcgttttatc tgcatttcca tcacctgtcg
      781 gcatattttt aagggctgat gcgtaattcc tcaatggagc atcagtgttg atggaaacga
      841 agatagacat tcatttaatc aataaaagtg atcaaaatca catcaattga aacgctgttt
      901 tatttttata ggaccattgt ttttattttg gaattgatca caaaaaaacc accgccgttg
      961 gcgatggttc attttttaag ctgatatgcg gcctatacgc ctcattgcag caggcggcaa
     1021 ataattattt tttcgccgca aaacgtgccg ctgcttcgtc ccagttcacc acgttccaga
     1081 actctttgat gtagtccgga cggcggttct ggaatttcag gtagtaagca tgttcccaca
     1141 catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc atcagcggag
     1201 aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc agccatgccc
     1261 agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag ttatcaacgg
     1321 agccgaagtc acgttcgata gccgctttca ggtcaccctg cagggtggtg ccttttttca
     1381 gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc agtacggttt
     1441 tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc aggttggcaa
     1501 attctggcag gctttccagc gccgcgttgg cgttgtttac gtaggtctga tggtgtttgg
     1561 tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg taagcatacg
     1621 gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg ccgattgtta
     1681 atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa tgattatcaa
     1741 tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt gaagcaaatc
     1801 acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa
     1861 tcgacagcac gatttttaca ctcatctcgt cggagatgtg aagcgacgaa aaatgatgag
     1921 gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat cggcgcggcc
     1981 agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg
     2041 tggtcagtcg gtgggattgt ttcgtggatt attctgccgt gggccatcag cgccctgtta
     2101 ctaccggatt tctgggcgta ttacagctcg tttagtctct ctacgctgct gcctgttttt
     2161 ctgttcggcg ctatgtgggg gatcggtaat atcaactatg gcctgaccat gcgttatctc
     2221 ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg tacgctgatg
     2281 acgccaatta tcaacggcaa tttcgatgtg ctgattaaca ccgaaggcgg acgcatgacg
     2341 ttactcggcg ttctggtggc gctgattggc gtagggattg taactcgagc cgggcagttg
     2401 aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg gctggtgctg
     2461 gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc cgcaaaaccg
     2521 atgcatgaag ccgctgccgc acttggcgtc gatccactgt atgttgctct gccaagctat
     2581 gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgttttat tcgtctggca
     2641 aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct gatcattcac
     2701 aatgtgttac tctcggcact gggcgggttg atgtggtatc tgcaattctt tttctatgcc
     2761 tagggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct gcatatgagt
     2821 ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa caatacagga
     2881 cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc cgctaacatc
     2941 gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca ctggctgggc
     3001 gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc aaagccacat
     3061 tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg cgcatgacac
     3121 actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg aaattgctga
     3181 cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc aaagggactt
     3241 ttcaggctag ccgccagccg ggtaatcagc ttatccagca acgtttcgct ggatgttggc
     3301 ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg cccgaacagc
     3361 aactcagcca tttcgttagc aaacggcaca tgctgactac tttcatgctc aagctgaccg
     3421 ataacctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg ttgccctgcg
     3481 ctggcgctaa atcccggaat cgctccctgc cagtcaagat tcagcttcag ccgctccggg
     3541 caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg tttatcgtca
     3601 gcatgaatat aaaagagatc gccacgggta atgcgataag gacgatcgtt gagtacatgc
     3661 agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg ttcggcaaag
     3721 acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc aaaaaaatca
     3781 tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga acgaagttga
     3841 ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca gaaagccatc
     3901 ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa
     3961 gtggttactg tcgctgaatc cacagcgata ggcgatgtca gtgacgctgg cctcgctgtg
     4021 gcgtagtaga tgtcgggctt tcatcagtcg caggcggttc aggtatcgct gaggcgtcag
     4081 tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa attgatccgc
     4141 cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca gaagcaagtt
     4201 gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca gcaagagcag
     4261 taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt ccccttcctg
     4321 ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca
     4381 gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc cggcgagaaa
     4441 ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat cggtatgttc
     4501 atacagatgc cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga tggtataggg
     4561 ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt catgaaaatc
     4621 atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt
     4681 acaagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct gatgtcgtca
     4741 acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca
     4801 cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc
     4861 acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
     4921 tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
     4981 gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg
     5041 gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc agcctgacgc
     5101 tgcgcgaaat ccatcgtttt aacaatgggc tgcatagcca aaacggttat gtcacctggg
     5161 atgtggatag cctggaaagt gccattcgcc ttggattaaa caaggtgtgc gaggaaggga
     5221 ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg ctcgaccaac
     5281 agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgcaccaat ggcctgatgc
     5341 cgcaggcaca acagcaactc ggcaaacgcg atatttatca gcgtagcggc atccagtttc
     5401 tacccttcaa tacgctttat cagttgcgtg cgctgacgga gcaacaacct gaacttattc
     5461 cacacattgc tcacgctctg ctgatgccgg attacttcag ttatcgcctg accggcaaga
     5521 tgaactggga atataccaat gccaccacca cacaactggt caatatcaat agcgacgact
     5581 gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc
     5641 atccgggtaa tgtcattggg cactggattt gcccgcaggg taatgagatt ccggtggtcg
     5701 ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg
     5761 ccgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag acgccattta
     5821 ccaatgacac ggcgctggca gccaacatca ccaatgaagg cggggcggaa ggtcgctatc
     5881 gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag gaacggcaaa
     5941 tcaacgatct cccggcgctt atcgccgcga cacaggcact tccggcctgc cgcttcatca
     6001 tcaatcccaa tgacgatcgc tttattaatc ctgacgagat gtgcagcgaa attcaggctg
     6061 cgtgtcggga aacggcgcaa ccgatcccgg gaagtgatgc tgaactggcg cgctgtattt
     6121 tcgacagtct ggcgctgctg tatgccgatg tgttgcatga gctggcgcag ctgcgcggtg
     6181 aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacacgctg ctcaaccagc
     6241 tatgcgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc tcaacgctcg
     6301 gcaatatcgg catcgagtta atgacgctgg atgaactcaa caatgtggat gatttccgtc
     6361 aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac agtgaaattg
     6421 cccactatgt ggcgcagatt cactctacac gacagacaaa ggagctttgc gcatgaccac
     6481 tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcggtgg ggattgatgt
     6541 cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct ggcagggcga
     6601 tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggggggattc aggccacagg
     6661 caattatccg ggcaaagcgc gtaatgccag tgagctacgt gccgatctgg aacaggctat
     6721 gcggctgatt ccggggccga aacggcttaa tttacatgcc atctatctgg aatcagatac
     6781 gccagtctcg cgcgaccaga tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa
     6841 agccaatcag ctcggtctgg attttaaccc ctcctgcttt tcgcatccgc taagcgccga
     6901 tggctttacg ctctcgcatc ccgacgacag cattcgccag ttctggattg atcactgcaa
     6961 agccagccgt cgcgtttcgg cctattttgg cgagcaactc ggcacaccat cggtgatgaa
     7021 catctggatc ccggatggta tgaaagatat caccgttgac cgtctcgccc cgcgtcagcg
     7081 tctgctggca gcactggatg aggtgatcag cgagaagcta aaccctgcgc accatatcga
     7141 cgccgttgag agcaaattgt ttggcattgg cgcagagagc tacacggttg gctccaatga
     7201 gttttacatg gggtatgcca ccagccgcca gactgcgctg tgcctggacg ccgggcactt
     7261 ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg taccgcagtt
     7321 gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtagtgc tgctggatga
     7381 tgaaacccag gcaattgcca gtgagattgt gcgtcacgat ctgtttgacc gggtgcatat
     7441 cggccttgac ttcttcgatg cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg
     7501 caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct accgctgagc tgcgcaagct
     7561 ggaagcggcg ggcgattaca ctgcgcgtct ggcactgctg gaagagcaga aatcgttgcc
     7621 gcggcaggcg gtctgggaaa tgtattgcca acatcacgat acgccagcag gtagcgaatg
     7681 gctggagagc gtgcgggctt atgagaaagc gattttgagc cagcgtgggt aaacactgcc
     7741 ggatgcggca cgagcgcctt atccggccta cgggtcgaat gtccatgcgg gctgtcaccc
     7801 tcaccccggc cctctccctg aaagggcgag ggagaaaaac atagcctcgt gcggcaacgg
     7861 agctgaacac cgctccggac agtcccctcg cccctacggg gagagggtta gggtgagggg
     7921 aacaagccgc accatgtcta aacaaggcaa cctaatttac aggaacacag aacatgcaaa
     7981 acattaccca gtcctggttt gtccagggaa tgatcaaagc caccaccgat gcctagctga
     8041 aaggctggga cgagcgcaac ggcggcaacc tgacgctgcg cctggatgac gccgatatcg
     8101 cgctttatca cgacaacttc cacccacaac cgcgctatat tccgctcagc caacccatgc
     8161 ctttactggc aaatacgccg tttattgtca ccggctccgg caaattcttc cgtaacgtcc
     8221 agctcgatcc tgcagctaac ttaggcgtcg taaaagtcga cagcgatggc gcgggctacc
     8281 atattctctg ggggttaacc aacgaagccg ttccaacttc tgaacttccg gctcactttc
     8341 tctcccactg cgagcgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc
     8401 acgccaccaa cctgatcgcc ctcacctatg tactggaaaa cgacaccgcg gtctttactc
     8461 gccaactgtg ggaaggcagc accgaatgtc tggtagtatt cccggatggc gttggcattc
     8521 tgccgtggat ggtgcccggc acggacgaaa tcggccaggc gaccgcacaa gagatgcaaa
     8581 aacattcgct ggtgttgtgg cccttccacg gtgtgttcgg ttccggaccg acgctggatg
     8641 aagcctttgg tttaatcgac accgcagaaa aatcagcaca agtattagtg aaggtttatt
     8701 cgatgggcgg catgaaacag accatcagcc gtgaagagtt gatagcgctc ggcaagcgct
     8761 tcggcgttac accactagcc agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag
     8821 cctttgtcat gcaggtaaac cccgacgccc acgaagagta tcagcgtcgg cataatccca
     8881 tctggccgga actggaagca gtgttgaaat ctcacggtgt gcataactac gccatttacc
     8941 tcgacaaagc gcgtaatctg ctgtttgcca tggtagagat tgaatctgaa gaacggtgga
     9001 atgcggtagc cagtactgat gtttgccaac gttggtggaa atatatgacc gatgttatgc
     9061 ccgctaaccc agataacagc ccggtgagta gcgagctgca agaagtgttt tacctgccgt
     9121 aattgcccaa tgtgccggag atgcctccgg cacaatattt taaatttcga tttgaaaaat
     9181 taaacatatt acatctcccg gtaagttata tttccctgat acattgtgag taaatcacaa
     9241 aaataatgaa taacccatta atgattcatg tggtttattt aaataaccat tatgtgcatt
     9301 actccgcaaa tctgaccttt cactctgtct caaattgtca ttgtttaccg acatatcgcc
     9361 tctcatcact taatcgctcc accattacaa ttgattaaaa tatattttat ggagtccgat
     9421 taatggcagc tcttactgca agctgtattg acctgaatat tcagggcaat ggcgcttatt
     9481 ccgttctgaa gcagttggcg acaatagcgt tacaaaacgg ttttatcacc gactcacacc
     9541 agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc tactggattt ggttccggtg
     9601 tcgcagtgcc gcacggtaaa agcgcctgcg ttaaacaacc gttcgtatta ttcgcccgca
     9661 aagcgcaggc tattgactgg aaagccagcg atggcgaaga cgtcaattgc tggatctgcc
     9721 tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat catcggcaca ctgtgtcgca
     9781 aaattattca ccaggaattt attcatcaac tgcaacaggg cgataccgac caggtgctgg
     9841 ccttgttaaa tcaaaccctc agctcataag gaagtggcga tggagtcatc cttacgtatt
     9901 gtcgcgatca ccaactgccc cgccgggatc gctcacacct acatggtggc ggaagccctg
     9961 gaacagaaag cccgttctct cggccatacc ataaaagtgg aaactcaagg gtccagtggc
    10021 gttgaaaacc gcttatccag cgaagagatt gccgctgccg attacgtcat tcttgctacc
    10081 gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg ggaagaaagt ttatgagatt
    10141 gccatctccc tggcgttgaa aaatatcgac cagattttca gcgaattacc gacaaactcg
    10201 cagctttttg ccgcagatag cggcgtgaag cttggcaaac aggaagtgca aagcggcagc
    10261 gtgatgagcc acctgatggc gggcgtctct gcggcgctgc cgtttgtcat cggcggtggt
    10321 atcctggtgg cgcttgccaa catgctggtg cagttcggtt tgccgtatac cgatatgtcg
    10381 aaaggcgcgc cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg cttcaccttt
    10441 atgatcccca tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc ggctttcgct
    10501 ccggcgtttc tggtttgcta tctggcgaac gacaaagcgc tgctcggcac ccagtcgggt
    10561 gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc
    10621 cgcaaagtgc gccttggcaa agcattacaa ccgctgctcg gttcaatgct gatcccgttc
    10681 gttactctgc tggttttcgg cgtgctcact tactacgtta tcgggccggt gatgtccgac
    10741 ctcatgggcg ggctgctcca cttcctgaat accattcctc catcaatgaa gtttgcggca
    10801 gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc
    10861 tggttcttct gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc
    10921 gttgtcgcgt tgatgccgcc cgttgcggcg ggtcttgcga cgttcattgc gcccaaactg
    10981 tttacccgac aggaaaaaga ggctgccagt agtgccattg tggtcggtgc cacagtcgca
    11041 accgaaccgg ctatacctta cgctctggca gctccgttgc cgatgattac cgccaatacg
    11101 ctggcgggcg gcattaccgg cgtgctggtg atcgctttcg gaatcaaacg tctggcacct
    11161 ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc cggtaggttc gttttatctg
    11221 gtgctggcga ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa aggattgtgg
    11281 ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg agttactgca
    11341 acagttgtgc gaagccagcg ccgttagcgg cgatgaacag gaagttcgcg acatcctgat
    11401 aaacacgctg gaaccttgcg ttaatgaaat cacctttgat ggtctgggca gctttgttgc
    11461 ccgtaaggga aataaaggtc caaaagttgc cgttgtcggg catatggatg aagtcggctt
    11521 tatggtcacc cacatcgacg agagcggttt tctgcgtttc accaccattg gtggctggtg
    11581 gaatcagtcg atgctcaacc atcgggtaac catacgcaca cacaagggag tgaaaatccc
    11641 tggtgtgatt ggtttcgtcg cgcctcatgc gttaacggaa aagcaaaggc aacagccgct
    11701 gtcatttgat gagatgttca ttgatattgg cgcaaacagt cgcgatgagg tggaaaaacg
    11761 cggtgtggcg atgggcgatt ttattagccc ggaagccaat tttgcctgct ggggcgaaga
    11821 taaagtggtc ggcaaggcgc tggataa
//
LOCUS       DAJTYL010000112        11521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYL010000112 DAJTYL010000000
VERSION     DAJTYL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.112"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..240)
                     /locus_tag="OO572_003163"
     CDS             complement(<1..240)
                     /locus_tag="OO572_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6662069.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA"
     gene            481..1281
                     /locus_tag="OO572_003164"
     CDS             481..1281
                     /locus_tag="OO572_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6662070.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1359..2129
                     /locus_tag="OO572_003165"
     CDS             1359..2129
                     /locus_tag="OO572_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6662071.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2177..3523)
                     /gene="mltD"
                     /locus_tag="OO572_003166"
     CDS             complement(2177..3523)
                     /gene="mltD"
                     /locus_tag="OO572_003166"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6662072.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(3595..4350)
                     /gene="gloB"
                     /locus_tag="OO572_003167"
     CDS             complement(3595..4350)
                     /gene="gloB"
                     /locus_tag="OO572_003167"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6662073.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4384..5106
                     /locus_tag="OO572_003168"
     CDS             4384..5106
                     /locus_tag="OO572_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6662074.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5103..5570)
                     /gene="rnhA"
                     /locus_tag="OO572_003169"
     CDS             complement(5103..5570)
                     /gene="rnhA"
                     /locus_tag="OO572_003169"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6662075.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5635..6366
                     /gene="dnaQ"
                     /locus_tag="OO572_003170"
     CDS             5635..6366
                     /gene="dnaQ"
                     /locus_tag="OO572_003170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6662076.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6499..6575
                     /locus_tag="OO572_003171"
     tRNA            6499..6575
                     /locus_tag="OO572_003171"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6533..6535,aa:Asp,seq:gtc)
     gene            complement(6706..7752)
                     /locus_tag="OO572_003172"
     CDS             complement(6706..7752)
                     /locus_tag="OO572_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6662077.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(7766..8325)
                     /gene="tssG"
                     /locus_tag="OO572_003173"
                     /pseudo
     CDS             complement(7766..8325)
                     /gene="tssG"
                     /locus_tag="OO572_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8322..10205)
                     /gene="tssF"
                     /locus_tag="OO572_003174"
     CDS             complement(8322..10205)
                     /gene="tssF"
                     /locus_tag="OO572_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6662078.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10221..10715)
                     /gene="tssE"
                     /locus_tag="OO572_003175"
     CDS             complement(10221..10715)
                     /gene="tssE"
                     /locus_tag="OO572_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6662079.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(10712..11518)
                     /locus_tag="OO572_003176"
                     /pseudo
     CDS             complement(10712..11518)
                     /locus_tag="OO572_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
BASE COUNT         2625 a         3030 c         3096 g         2770 t
ORIGIN      
        1 tgccgctgcc atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc
       61 cgtcaggcta agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag
      121 ctttttcacc gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct
      181 aaagctgccg ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat
      241 ttttgctcct gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag
      301 gatgcgtgat atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt
      361 aagcacttgc ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta
      421 taacagtaga accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa
      481 gtgcgaaaaa acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta
      541 ccgccggggt cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc
      601 gaagagcgta ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg
      661 tcggtattaa agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca
      721 acgccagagt tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct
      781 ttcgtgctgc cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat
      841 tgctgcccgt tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg
      901 gtctatccat tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc
      961 agtctgggcg tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac
     1021 cacagcggcc cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac
     1081 gcgttatatc gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag
     1141 cgccgtcggg cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc
     1201 gaagcttctg tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt
     1261 cccggcaagc ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc
     1321 gctgtccttt tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg
     1381 accacgtaga aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg
     1441 catccggacg agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg
     1501 cacttgatat gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg
     1561 cggtggtggc gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag
     1621 cccggcaact gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg
     1681 ataacgcatt tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg
     1741 cagcactgcg tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg
     1801 taatgtctcc gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg
     1861 atacctctca cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca
     1921 atctgatagt tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg
     1981 cgagaatgcg tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat
     2041 cgacagaggt gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca
     2101 tcatggtaga agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt
     2161 tttattatct ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct
     2221 tgtcgcctgg ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca
     2281 cgccgtggcg tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc
     2341 tatcgctgtt gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct
     2401 tagatccgcg cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag
     2461 cgatgcttga aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat
     2521 tgttggcaac cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca
     2581 gttgatctgc atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg
     2641 agcctttcac gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg
     2701 caaccttcgc catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat
     2761 cggtcgttgg cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca
     2821 gcattttagg cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg
     2881 atttcccacg cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat
     2941 aagccgctac ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat
     3001 tcagcgcggc agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt
     3061 tcaaaccata attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc
     3121 cagacgttgc gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag
     3181 gcatgttacg ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag
     3241 ttacatcgtg gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt
     3301 cattttccgg aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg
     3361 gcgcgataga cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc
     3421 cttgaccagc tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac
     3481 ccacgagcag gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg
     3541 cttaaaagac gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa
     3601 cctatctttc tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc
     3661 ttcattaatt ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg
     3721 ctcatttttc agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt
     3781 acgataataa tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt
     3841 tgataacgta tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt
     3901 tttaagtgat tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga
     3961 aaacagcgtg tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt
     4021 gtgacccggt gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc
     4081 tttgactacc tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg
     4141 cggaaacttt tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag
     4201 aaatatggcc tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc
     4261 tccgggatcg acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc
     4321 atcaaaggcg ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt
     4381 gtgatgaaac cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg
     4441 ccctggggag agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa
     4501 atgtatggtt ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg
     4561 tgcgcggttt ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg
     4621 gatccacttc atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc
     4681 ttgccgtggt gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat
     4741 gatggctggc tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt
     4801 gtgccggtat tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa
     4861 ctggactggc tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc
     4921 ccgtggaaca aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta
     4981 caacttattg ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt
     5041 aaaaacaagc cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag
     5101 gcttaaactt cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga
     5161 gccagttcat cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat
     5221 ttgatttgat gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact
     5281 ggttttttgt ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata
     5341 ccctggcgga catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc
     5401 tccagcgcga caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct
     5461 gcgctaaagg ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca
     5521 ggattaccca gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga
     5581 cttcctgtaa ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc
     5641 actgcaatta cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt
     5701 gcacactatg aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc
     5761 ctgacgggca ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc
     5821 tttggcgtac atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta
     5881 gccgatgagt ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc
     5941 gatataggct ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat
     6001 accttctgta aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc
     6061 aacagcctcg atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac
     6121 ggggcattac tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa
     6181 acgtcgatgg cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt
     6241 cagcgcattg tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg
     6301 gcagcacatg aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg
     6361 gcataaatac atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga
     6421 ttcaaaagat gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt
     6481 cccaacggaa cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca
     6541 gggggtcgcg ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa
     6601 tgattcgggc tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat
     6661 atgatttaaa aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc
     6721 ccatttctcc agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac
     6781 caggcgttct gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa
     6841 ccagcgacgg atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg
     6901 ttctgcgccg gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc
     6961 tgcctgcatc tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg
     7021 tggcaggatc tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt
     7081 cagacgggag aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg
     7141 ctcagtggtc tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag
     7201 gtctgccagt tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga
     7261 tagcgaacgc tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat
     7321 gccacgaata tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg
     7381 tgccaccgca gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc
     7441 gtccgggtac gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc
     7501 accggcctgc tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg
     7561 catcagcaga cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact
     7621 gacaaaatcg ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta
     7681 ttccggatcg tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag
     7741 gaatatttcc atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc
     7801 tggactcacg caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt
     7861 cagcgctctc ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat
     7921 gttgcggttt atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact
     7981 gcgtcagcca gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg
     8041 gttacgcgcc agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag
     8101 gctgtacaga ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg
     8161 cgcaaaggtc agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt
     8221 gttgcccgcc ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc
     8281 gtaacgccag gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt
     8341 cccgccctga caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc
     8401 gagaaggtat taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga
     8461 tacggactga tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc
     8521 ccatagacca gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca
     8581 atcaggctgc gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc
     8641 agatctcgca acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta
     8701 aaagataact ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc
     8761 gagcgtggcg ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga
     8821 tcgtcccggc cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg
     8881 atggtgatgt ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg
     8941 aacacttccg agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag
     9001 cggcggggct cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg
     9061 gtgtggtaga gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact
     9121 tcctggaccg agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt
     9181 tccgtcgtgc tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc
     9241 ggggtacaga acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc
     9301 aggcggttca gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca
     9361 gacaggcctg tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc
     9421 aggttatgac cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg
     9481 ccttcgtaca taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga
     9541 atgccagcca ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta
     9601 cgctcctcgc cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt
     9661 agttcaccaa aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc
     9721 aggtagcggt gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg
     9781 gcgagcggcc acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct
     9841 tccggaatgg cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg
     9901 ccttcttccg tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa
     9961 ttggggctga ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt
    10021 agctgcgtgc gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg
    10081 gcaatatccg ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat
    10141 tcggctcccg tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga
    10201 tccatcggtc tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg
    10261 ctacgtaccc ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac
    10321 tgtaatgtgt tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg
    10381 gacgctttca gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg
    10441 tcccacttgt gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg
    10501 gacgctgccg cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg
    10561 agcagaaatg acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata
    10621 ccgtggtcgc gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc
    10681 aggtgattgc ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa
    10741 aggtacaggt ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct
    10801 gacccagcgc ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca
    10861 gacgcaggga gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt
    10921 gtgtatcgcc attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg
    10981 caggccgcgg cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc
    11041 ccgcgatcag ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg
    11101 cgccgccatt ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag
    11161 catcagcttc ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca
    11221 tccaggcggg cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc
    11281 ggtatatctc acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt
    11341 ccatccgggc gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca
    11401 ataactgaaa caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct
    11461 ctgccatctg aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg
    11521 g
//
LOCUS       DAJTYL010000113        11451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYL010000113 DAJTYL010000000
VERSION     DAJTYL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.113"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..732
                     /locus_tag="OO572_003177"
     CDS             <1..732
                     /locus_tag="OO572_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662080.1"
                     /translation="LIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDE
                     LKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNA
                     EMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDES
                     GKEQFSGAVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPL
                     TQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(935..1288)
                     /locus_tag="OO572_003178"
     CDS             complement(935..1288)
                     /locus_tag="OO572_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6662081.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1426..3072)
                     /gene="groL"
                     /locus_tag="OO572_003179"
     CDS             complement(1426..3072)
                     /gene="groL"
                     /locus_tag="OO572_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6662082.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3116..3409)
                     /locus_tag="OO572_003180"
     CDS             complement(3116..3409)
                     /locus_tag="OO572_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6662083.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3685..4941
                     /gene="yjeH"
                     /locus_tag="OO572_003181"
     CDS             3685..4941
                     /gene="yjeH"
                     /locus_tag="OO572_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6662084.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4957..5433)
                     /gene="fxsA"
                     /locus_tag="OO572_003182"
     CDS             complement(4957..5433)
                     /gene="fxsA"
                     /locus_tag="OO572_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6662085.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5770..7206
                     /gene="aspA"
                     /locus_tag="OO572_003183"
     CDS             5770..7206
                     /gene="aspA"
                     /locus_tag="OO572_003183"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6662086.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7324..8625
                     /gene="dcuA"
                     /locus_tag="OO572_003184"
     CDS             7324..8625
                     /gene="dcuA"
                     /locus_tag="OO572_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6662087.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8741..9079
                     /gene="cutA"
                     /locus_tag="OO572_003185"
     CDS             8741..9079
                     /gene="cutA"
                     /locus_tag="OO572_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6662088.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9055..10752
                     /gene="dsbD"
                     /locus_tag="OO572_003186"
     CDS             9055..10752
                     /gene="dsbD"
                     /locus_tag="OO572_003186"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6662089.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10789..11364
                     /locus_tag="OO572_003187"
     CDS             10789..11364
                     /locus_tag="OO572_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6662090.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2731 a         2981 c         2831 g         2908 t
ORIGIN      
        1 ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt
       61 cagtatgagc aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt
      121 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
      181 gataatcaag gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag
      241 ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
      301 aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta
      361 tacgacgttg actgccagga caatggcaat ctggaatatg acacctattc ccaaccggaa
      421 tggaaacata acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt
      481 ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa
      541 atagaggcga tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt
      601 gtgccatgtc aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag
      661 gatcaatgct tacgcgcgct gcatcactta cgtgtacaat ccaccagcaa aactgcacca
      721 caggcgaaat aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact
      781 gataacgtga tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt
      841 ctcaagcatt caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc
      901 aataatacgt ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac
      961 cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga
     1021 tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc
     1081 cgcgcataga accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg
     1141 tcgcagtacc aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct
     1201 gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga
     1261 caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca
     1321 ctcgcgtcat ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga
     1381 catttctgcc cgggggtttg tttatttctg cgaagtgcag ggcaattaca tcatgccgcc
     1441 catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg
     1501 caggtcggta accatgcatt cggtggtgat catcaggcca gccacagaag ctgcgtactg
     1561 cagagcagaa cgggttactt tggttgggtc caggataccc atgtcgatca tgttgccgta
     1621 ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac
     1681 aacagacggt tcttcgccgc agttcaggac gatctgacgc agcggagctt ccattgcacg
     1741 cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt
     1801 agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc
     1861 acgggtcgcg tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt
     1921 agcagcacct actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag
     1981 tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg
     2041 gccctggatt gcagcttctt cacccacgcc atcgatgata gtggtggtgt ctttgttgat
     2101 cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat
     2161 ctcttcagag attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg
     2221 acgatcgccg aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt
     2281 aacaaccaga gttgctagcg cttcgccttc aacatcttca gcgatgatca gcagcggttt
     2341 gcctgctttg gcaacagctt ccagaaccgg cagcatttcg cggatgttgg agattttctt
     2401 gtcagccagc aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat
     2461 gaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc
     2521 ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc
     2581 ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc
     2641 aatcgcttta gaatcagagc acggtacgga cagcgctttc agttcttcaa ctgcagcggt
     2701 aaccgctttg tcgataccac gtttcaggtc catcgggttc atgcccgcag caacagcttt
     2761 cagaccttca gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc
     2821 agcgtcgttc gctttggagg caacttcttt caccatctgc gcacccatat tttcgaactt
     2881 gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc
     2941 gaaagattta tccagaacta cgttacggcc ttttggaccg agggtaactt tcactgcatc
     3001 tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc
     3061 tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg
     3121 cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct
     3181 tctcggattt cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacgtcca
     3241 gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc
     3301 gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa
     3361 cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc
     3421 ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg
     3481 tgaccagaga aatggggatg aggcttcgcc ccttcaaggg ggaaaaagaa aaaaaattct
     3541 gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca
     3601 tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat
     3661 ttgggcgcaa tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag
     3721 ggcattggcc tgctatcgac gtcattatta ggcactggcg tgtttgccgt tcctgcgtta
     3781 gctgcactgg tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg
     3841 ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc aggcggcgtc
     3901 gcgcacttcg tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggctgttt
     3961 ttatcggtca ttcccgtggg tttgcctgcc gcactacaaa ttgccgccgg attcggccaa
     4021 gcaatgtttg gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgttg
     4081 gtgtggtata tcggtactct cggtgccagt tccagtgcta atctacaaac cgttattgcc
     4141 ggacttatcg tcgcactgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgagt
     4201 atccccttcc ccgcgccagg aaatatcgaa cttaccgggt tatttgctgc gttatcagtg
     4261 atgttctggt gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat
     4321 ccagagcgtg attttcctcg tgctttgatg attggtctgc tgctggcagg attagtctac
     4381 tggggctgta cggtagtcgt cttacacttc gacgcctatg gtgaacaaat ggcggcggca
     4441 gcatcgcttc caaaaattgt agtacagttg ttcggtgtag gagcgttatg gattgcctgc
     4501 gtaattggct atctggcctg ctttgccagt cttaacattt atatacagag cttcgcccgc
     4561 ctggtctggt cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc
     4621 catatcccga ataatgccct caatgcggtg cttggctgct gcgtggtgag cacgttggta
     4681 attcatgcct tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt
     4741 atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggtcg ttatcgacta
     4801 ctggcggtgg ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt
     4861 ctctatgcgc tgatcatgct ggtggggtta tggctgtttc tgccaaaacg aaaaacgccg
     4921 gaaaatggca taaccacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg
     4981 atcaaggcgg tcgcggtcat catcctttcg ctgatactca ccatcaaaag tattaccgcc
     5041 accggtcccg gcgctaaaac cgccgcctgg catgcgagaa aagcgcaaat gtggcatcaa
     5101 cttcactgtc agatgctttt gcaccggcgg caataaaagc agaagaccga ggaagtcggt
     5161 gaaaaagccc ggtaataaaa gcagcaaacc ggcaataatc agcgaaacac ttttaatcat
     5221 ctccgccgca gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa
     5281 gccctggtta cgtaccagtg acataccgat aactgacgtg aatataacca gcacgagggt
     5341 cagcaatacc cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata
     5401 aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggtt
     5461 aaacccggct acctgaatgg gttgcgaatc gccttcagct tatattgtgg tcattagcaa
     5521 aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca
     5581 caaattaata attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat
     5641 cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa
     5701 aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag
     5761 aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt
     5821 ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc
     5881 aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc
     5941 gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt
     6001 gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc
     6061 taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc
     6121 ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat
     6181 gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac
     6241 tcttccctga ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa
     6301 gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg
     6361 atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac
     6421 atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact
     6481 ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact
     6541 ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat
     6601 gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac
     6661 ctgcgcttgc tctcttctgg tccacgtgcc ggcctgaacg agatcaacct gccggaactg
     6721 caggcgggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac
     6781 caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt
     6841 cagctgcagt tgaacgttat ggaaccggtc attggccagg ccatgttcga atccgttcac
     6901 attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac
     6961 aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg
     7021 ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt
     7081 gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc
     7141 tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa
     7201 cagtaatcgt acagggtagt acaaataaaa aaggcacgtc agatgacgtg ccttttttct
     7261 tgtgagcagt aacttaaaaa taacaatcta atatcaactt gttaaaaaac aaggaaggct
     7321 aatatgctag ttgtagagct catcatagtt ttgctggcga tcttcttggg cgccagattg
     7381 gggggaatag gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc
     7441 gttaaacccg gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct
     7501 atttctgcca tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg
     7561 ctgcgccgta acccgaaata catcacgatc ctcgcaccga tcgtgaccta tttcctgact
     7621 atctttgctg gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg
     7681 aaggaacaag gcgttaaacc ttgccgtccg ctctctactg cagtggtatc cgcgcagatt
     7741 gcgatcaccg catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt
     7801 catggcatca gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt
     7861 ctggtgatgt ccttcctggt cactatgctg ttcaactcca aactctctga cgatccgatt
     7921 tatcgcaagc gtctggaaga gggcttggtt gaactgcgcg gtgaaaagca gattgaaatc
     7981 aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc
     8041 tatgcaatca tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc
     8101 aacgcaatcc tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc
     8161 gataccgaca acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt
     8221 atcctgggtg ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa
     8281 gataccgctg gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt
     8341 gcttctgctc tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg
     8401 gcactgaacg tttcaccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc
     8461 attctgccga cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc
     8521 cgtatcggta aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc
     8581 ctggccgttt gcttcggctt cgtgctgggt agcttcatgc tgtaatgatc aatcgcgggg
     8641 cgttcacgcc ccgctttctt tcccgccgac taacatcctt cccccgtccg ttgtatagtg
     8701 acctctctct tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc
     8761 gaataccacg tctgtcgtgg tgctatgtac cgcaccggat gaagcgacag cccaggattt
     8821 agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac
     8881 ctctctctat tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa
     8941 aactaccgta tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca
     9001 aaccccggaa cttctggttt tacctgttac acacggagac acagattacc tctcatggct
     9061 caacgcatct ttacgctgat tctgctactt tgcagcactt ccgtttttgc cggattattc
     9121 gacgcgccgg gacgttcaca atttgtcccc gcggatcaag cctttgcttt tgattttcag
     9181 caaaaccaac atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt
     9241 aaacagatcc gcattacgcc ggaacacgcg aaaattgccg acgagcagct gccgcaaggc
     9301 gtctggcatg aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt
     9361 cccgtaacca tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt
     9421 gctgatgccg gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc
     9481 gccaacaacg cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca caccgcgcaa
     9541 ttgccctttt ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc
     9601 gtgctgccaa tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc
     9661 actgccagag cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg
     9721 gcgctgggtc tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca
     9781 tacgtgctca ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg
     9841 tttactctac aactcccctc ttcgctgcaa acacgtctca cgttgatgag caatcgccaa
     9901 cagggcggct cacctagcgg tgtgtttgtt atgggggcga ttgccggact gatctgttca
     9961 ccatgcacca ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg
    10021 tggccgggcg gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta
    10081 attaccgtct ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa
    10141 accgcgtttg gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt
    10201 gatgtatggg gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt
    10261 atcaccagcc tacaggctaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca
    10321 gcggcattgg ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg
    10381 caaactcaaa cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg
    10441 ctcgttgaag ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc
    10501 tgtaaagagt ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac
    10561 acggtcttac ttcaggccaa cgtcacggcc aacgacgcac aagatgtggc gctgttaaag
    10621 catcttaatg tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat
    10681 ccacaagcac gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat
    10741 cgccaaccgt gaacgacact tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa
    10801 gatgtactgg gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg
    10861 gagatggttg ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac
    10921 aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt
    10981 cgtcagctga tgctggacga aacgcaaacc gccgaacaaa agctgctggc acgttatcag
    11041 gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt
    11101 acgttttatc ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgct
    11161 gcctacgatt tcacccacga actgttaacc acgctggaag ttgacgatcc ggcgatggta
    11221 gcaaagcaga tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc
    11281 caggcggatg tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc
    11341 cgccagggtg gtgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt
    11401 aagcagttga atcgctttta ctgaaattag gttgacgaga tgtgcagatt a
//
LOCUS       DAJTYL010000114        11390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYL010000114 DAJTYL010000000
VERSION     DAJTYL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.114"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(225..500)
                     /gene="yjfN"
                     /locus_tag="OO572_003188"
     CDS             complement(225..500)
                     /gene="yjfN"
                     /locus_tag="OO572_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6662091.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(649..978)
                     /gene="bsmA"
                     /locus_tag="OO572_003189"
     CDS             complement(649..978)
                     /gene="bsmA"
                     /locus_tag="OO572_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6662092.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1160..1909
                     /gene="yjfP"
                     /locus_tag="OO572_003190"
     CDS             1160..1909
                     /gene="yjfP"
                     /locus_tag="OO572_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6662093.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(1906..2661)
                     /gene="ulaR"
                     /locus_tag="OO572_003191"
     CDS             complement(1906..2661)
                     /gene="ulaR"
                     /locus_tag="OO572_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6662094.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2769..3833)
                     /gene="ulaG"
                     /locus_tag="OO572_003192"
     CDS             complement(2769..3833)
                     /gene="ulaG"
                     /locus_tag="OO572_003192"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6662095.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4188..5585
                     /gene="ulaA"
                     /locus_tag="OO572_003193"
     CDS             4188..5585
                     /gene="ulaA"
                     /locus_tag="OO572_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6662096.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5601..5906
                     /gene="ulaB"
                     /locus_tag="OO572_003194"
     CDS             5601..5906
                     /gene="ulaB"
                     /locus_tag="OO572_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6662097.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            5916..6380
                     /gene="ulaC"
                     /locus_tag="OO572_003195"
     CDS             5916..6380
                     /gene="ulaC"
                     /locus_tag="OO572_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6662098.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6394..7044
                     /gene="ulaD"
                     /locus_tag="OO572_003196"
     CDS             6394..7044
                     /gene="ulaD"
                     /locus_tag="OO572_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6662099.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7054..7908
                     /gene="ulaE"
                     /locus_tag="OO572_003197"
     CDS             7054..7908
                     /gene="ulaE"
                     /locus_tag="OO572_003197"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6662100.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            7908..8594
                     /gene="ulaF"
                     /locus_tag="OO572_003198"
     CDS             7908..8594
                     /gene="ulaF"
                     /locus_tag="OO572_003198"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6662101.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8723..8998)
                     /locus_tag="OO572_003199"
     CDS             complement(8723..8998)
                     /locus_tag="OO572_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6662102.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9325..9720
                     /gene="rpsF"
                     /locus_tag="OO572_003200"
     CDS             9325..9720
                     /gene="rpsF"
                     /locus_tag="OO572_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6662103.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9727..10041
                     /gene="priB"
                     /locus_tag="OO572_003201"
     CDS             9727..10041
                     /gene="priB"
                     /locus_tag="OO572_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6662104.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10046..10273
                     /gene="rpsR"
                     /locus_tag="OO572_003202"
     CDS             10046..10273
                     /gene="rpsR"
                     /locus_tag="OO572_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6662105.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10315..10764
                     /gene="rplI"
                     /locus_tag="OO572_003203"
     CDS             10315..10764
                     /gene="rplI"
                     /locus_tag="OO572_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6662106.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(10835..>11390)
                     /locus_tag="OO572_003204"
     CDS             complement(10835..>11390)
                     /locus_tag="OO572_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6662107.1"
                     /translation="NGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVES
                     FEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQLHT
                     VTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSDR
                     MHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2697 a         2809 c         3004 g         2880 t
ORIGIN      
        1 gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag
       61 atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact
      121 gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg
      181 cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc
      241 gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat
      301 gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct
      361 gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg
      421 gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt
      481 gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca
      541 ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac
      601 gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta
      661 caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac
      721 gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg
      781 agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg
      841 gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc
      901 gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt
      961 acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg
     1021 aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc
     1081 aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc
     1141 agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt
     1201 tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca
     1261 cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg
     1321 tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc
     1381 gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt
     1441 acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc
     1501 gtcgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac
     1561 cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct
     1621 gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga
     1681 gtgggaagcg acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg
     1741 cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga
     1801 gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac
     1861 gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat
     1921 gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat
     1981 cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact
     2041 gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc
     2101 tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat
     2161 ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact
     2221 tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt
     2281 tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag
     2341 cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga
     2401 ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg
     2461 cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt
     2521 gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg
     2581 ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg
     2641 atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt
     2701 tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa
     2761 acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg
     2821 aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg
     2881 ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac
     2941 agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg
     3001 ataaccactt tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg
     3061 tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg
     3121 ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg
     3181 ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca
     3241 gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca
     3301 tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct
     3361 ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg
     3421 tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga
     3481 tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg
     3541 gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc
     3601 tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa
     3661 acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca
     3721 aatgtgccag gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc
     3781 gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt
     3841 taccttcatg atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact
     3901 attccttcag gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt
     3961 ttgagtgggg aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa
     4021 taattgtttc gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca
     4081 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
     4141 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
     4201 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
     4261 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
     4321 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
     4381 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg
     4441 atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag
     4501 gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta
     4561 ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg
     4621 ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc
     4681 tggtttcgct ctactggggc attacctcca acatgatgta caagccgact caggaagtga
     4741 cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata
     4801 aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct
     4861 ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg
     4921 gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact
     4981 ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc
     5041 agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc
     5101 tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg
     5161 ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg
     5221 tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg
     5281 ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc
     5341 tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga
     5401 gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct
     5461 tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct
     5521 tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg
     5581 cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag
     5641 gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc
     5701 acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca
     5761 tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg
     5821 gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag
     5881 cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg
     5941 agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg
     6001 gtgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg
     6061 atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg
     6121 gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc
     6181 tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg
     6241 atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag
     6301 aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc
     6361 gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg
     6421 ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc
     6481 gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat
     6541 ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc
     6601 aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt
     6661 gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg
     6721 cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt
     6781 attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt
     6841 gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc
     6901 ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc
     6961 gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt
     7021 tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc
     7081 atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa
     7141 acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc
     7201 cttgactgga gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg
     7261 cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac
     7321 gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt
     7381 ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa
     7441 acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag
     7501 gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag caaggcgctg
     7561 ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg
     7621 tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg
     7681 gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg
     7741 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
     7801 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat
     7861 tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa
     7921 aacagcaggt gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt
     7981 ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg
     8041 ttgcctacga aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg
     8101 tggaagggga gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt
     8161 acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg
     8221 cggggctggc gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc
     8281 cgtgtacgcg tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca
     8341 aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt
     8401 atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag
     8461 tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc
     8521 atatcgacag ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt
     8581 acgggcagaa gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa
     8641 ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat
     8701 gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt
     8761 tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt
     8821 cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca
     8881 cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg
     8941 cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag
     9001 atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt
     9061 taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact
     9121 gtgacggaat gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat
     9181 ttatttctgc tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt
     9241 cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct
     9301 gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct
     9361 gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca
     9421 gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac
     9481 aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat
     9541 gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc
     9601 aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt
     9661 cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa
     9721 tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag
     9781 gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag
     9841 gaagccggct ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa
     9901 aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt
     9961 tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa
    10021 ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg
    10081 tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta
    10141 catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca
    10201 gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga
    10261 tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa
    10321 gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa
    10381 gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa
    10441 aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg
    10501 gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct
    10561 aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct
    10621 gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg
    10681 cgtaccactg gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg
    10741 atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt
    10801 ggtcggcgtt ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac
    10861 cattctcttg tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc
    10921 ttatgtctcg gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat
    10981 ttgataagga acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca
    11041 atgcagcaat gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga
    11101 tataaagatc gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg
    11161 ttacaacgta tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa
    11221 caacctcgtg tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat
    11281 atttcagttc tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa
    11341 tattatctaa tgtagcttca tggttccatc caaaagaatt atcaccattc
//
LOCUS       DAJTYL010000115        11155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYL010000115 DAJTYL010000000
VERSION     DAJTYL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.115"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(63..1208)
                     /gene="garK"
                     /locus_tag="OO572_003205"
     CDS             complement(63..1208)
                     /gene="garK"
                     /locus_tag="OO572_003205"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6662108.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1305..2195)
                     /gene="garR"
                     /locus_tag="OO572_003206"
     CDS             complement(1305..2195)
                     /gene="garR"
                     /locus_tag="OO572_003206"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6662109.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2225..2995)
                     /gene="garL"
                     /locus_tag="OO572_003207"
                     /pseudo
     CDS             complement(2225..2995)
                     /gene="garL"
                     /locus_tag="OO572_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3011..4345)
                     /gene="garP"
                     /locus_tag="OO572_003208"
     CDS             complement(3011..4345)
                     /gene="garP"
                     /locus_tag="OO572_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6662110.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            4720..6291
                     /gene="garD"
                     /locus_tag="OO572_003209"
     CDS             4720..6291
                     /gene="garD"
                     /locus_tag="OO572_003209"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6662111.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6343..7167)
                     /gene="agaR"
                     /locus_tag="OO572_003210"
     CDS             complement(6343..7167)
                     /gene="agaR"
                     /locus_tag="OO572_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6662112.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7416..8696
                     /gene="kbaZ"
                     /locus_tag="OO572_003211"
     CDS             7416..8696
                     /gene="kbaZ"
                     /locus_tag="OO572_003211"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6662113.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            8719..9192
                     /gene="agaV"
                     /locus_tag="OO572_003212"
     CDS             8719..9192
                     /gene="agaV"
                     /locus_tag="OO572_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6662114.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9203..9982
                     /locus_tag="OO572_003213"
     CDS             9203..9982
                     /locus_tag="OO572_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6662115.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9972..10781
                     /locus_tag="OO572_003214"
     CDS             9972..10781
                     /locus_tag="OO572_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6662116.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10868..>11155
                     /locus_tag="OO572_003215"
     CDS             10868..>11155
                     /locus_tag="OO572_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6662117.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQL"
BASE COUNT         2698 a         2988 c         2804 g         2665 t
ORIGIN      
        1 cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct
       61 tgtcaccccg tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg
      121 cagatattgt cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg
      181 ctgaagaccg catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc
      241 gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc
      301 ttgccgtgaa tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg
      361 tgaatatgtt cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc
      421 gcaccaagaa acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca
      481 tcttttacat caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg
      541 tccagctcaa caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg
      601 ccgttatcgc ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt
      661 aagcgcggat cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca
      721 aaaccaattt cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct
      781 gcgccgccat catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc
      841 tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt
      901 ttttccgcag gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg
      961 ccatcgccgg agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag
     1021 gcgtgacgtt cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg
     1081 tcggcaaccg gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct
     1141 atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg
     1201 attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg
     1261 gattccaggc ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg
     1321 actttcgcca gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca
     1381 tctgctcgca gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg
     1441 acgccgtggg aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag
     1501 cccggcttga agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc
     1561 gccagtccac cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc
     1621 agcgttaacg cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt
     1681 ttggtgacgt tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc
     1741 atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta
     1801 ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc
     1861 gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc
     1921 aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc
     1981 acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta
     2041 gccgtagagg ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg
     2101 tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata
     2161 atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg
     2221 gtggttattt tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat
     2281 cgctgccgac agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga
     2341 ccggcgcgag gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa
     2401 ttgctttttg tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat
     2461 cgctggggcc gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat
     2521 ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga
     2581 aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc
     2641 cttccggtgg gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa
     2701 aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt
     2761 tggtcggcac tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa
     2821 taaacgtgga gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag
     2881 ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta
     2941 cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt
     3001 atacctttgc ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc
     3061 agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca
     3121 gttcacttac caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga
     3181 cgttaaagac gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg
     3241 gccagcccag agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca
     3301 gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca
     3361 caatcggtag cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga
     3421 agacacctcc cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac
     3481 ccactttcag aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga
     3541 agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt
     3601 tagagagcaa ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct
     3661 ttttgtggtc catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag
     3721 acatacgtgg gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa
     3781 aacaaatcac ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc
     3841 caagcagagg cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg
     3901 cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg
     3961 atggagcttc cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag
     4021 ccagcgggaa catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc
     4081 tgtaggtgta aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca
     4141 tcagcaagta ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact
     4201 gcaactcttt tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa
     4261 cggcggtaac aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt
     4321 tttcgtcaac ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt
     4381 attatgggga aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga
     4441 attagtataa gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt
     4501 tttaaatatt attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct
     4561 ttaccttgcc gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt
     4621 ttaaactcct gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg
     4681 tcctcatttt aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga
     4741 caagaaacgc caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt
     4801 gttaatgata atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa
     4861 catattcccc aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt
     4921 cgttatggcg aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac
     4981 gaatcaatgg ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa
     5041 gtcccggaac ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat
     5101 ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc
     5161 gtggtggact atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc
     5221 gatggcgtgg tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca
     5281 gcagttgtac ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa
     5341 gtaatggtga ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg
     5401 gatgatgtgc aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag
     5461 catgtcggtt ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa
     5521 aaactgaatc aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat
     5581 tgcggtggca gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct
     5641 gatctactgg tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac
     5701 gcgatccatc tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag
     5761 gagatggagt ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct
     5821 tcgccgggca acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt
     5881 gctaaatcgg gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa
     5941 cgcggattaa tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg
     6001 gcttcgggta tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg
     6061 gcggtacccg tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta
     6121 atggatatta atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg
     6181 aagttgttcc actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa
     6241 tggggactgc ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat
     6301 tcgtattatg cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg
     6361 atccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg
     6421 aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact
     6481 ggaacgattg aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat
     6541 ccgacggctt aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat
     6601 cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg
     6661 atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac
     6721 gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc
     6781 agtgtacttg cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac
     6841 ccgatgacct ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt
     6901 gttcagtgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat
     6961 caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt
     7021 gcggatcgtc acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc
     7081 ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt
     7141 ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt
     7201 cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc
     7261 aagctactct cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact
     7321 attatctttc gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa
     7381 gatcaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga
     7441 gacagcacaa agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg
     7501 tgctggaggc tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa
     7561 cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg
     7621 gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg
     7681 gtgaccatct ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg
     7741 ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca
     7801 gcatgtcctg tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg
     7861 cccgtctggc gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg
     7921 agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc
     7981 tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg
     8041 aaaagcaagg tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg
     8101 tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc
     8161 agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc
     8221 cgcaatctct gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc
     8281 tgaccttcgc cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac
     8341 cagcgaaagc ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg
     8401 aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca
     8461 gctactcaga tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc
     8521 atctggttcg taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc
     8581 cgctgcagta cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta
     8641 taaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa
     8701 aacaaagagg aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt
     8761 cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc
     8821 aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa
     8881 gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc
     8941 gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa
     9001 ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa
     9061 caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa
     9121 gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt
     9181 aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat
     9241 tatcgccttt atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt
     9301 ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg
     9361 cgatacgctg gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa
     9421 cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga
     9481 tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct
     9541 gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac
     9601 tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct
     9661 ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct
     9721 gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt
     9781 tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt
     9841 tgttgccgca acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat
     9901 ggcgctgatc gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga
     9961 ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa
    10021 cactgctgac tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga
    10081 cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact
    10141 acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc
    10201 acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc
    10261 atccgtttct ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg
    10321 acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg
    10381 atgcgatgtt ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc
    10441 tacaaggctc tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg
    10501 ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta
    10561 ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg
    10621 gcgcactggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg
    10681 cagtagttaa gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg
    10741 tctacaccct gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg
    10801 cggttaccgt ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag
    10861 gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa
    10921 agcaatgaag caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac
    10981 ctcaagcgcc gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga
    11041 agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac
    11101 gctggcgatg caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactg
//
LOCUS       DAJTYL010000116        11029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYL010000116 DAJTYL010000000
VERSION     DAJTYL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.116"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1036)
                     /locus_tag="OO572_003216"
     CDS             complement(<1..1036)
                     /locus_tag="OO572_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6662118.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEEN"
     gene            1527..1658
                     /locus_tag="OO572_003217"
     CDS             1527..1658
                     /locus_tag="OO572_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6662119.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1918..2913
                     /gene="pgl"
                     /locus_tag="OO572_003218"
     CDS             1918..2913
                     /gene="pgl"
                     /locus_tag="OO572_003218"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6662120.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2954..3907)
                     /locus_tag="OO572_003219"
                     /pseudo
     CDS             complement(2954..3907)
                     /locus_tag="OO572_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4091..5143
                     /locus_tag="OO572_003220"
     CDS             4091..5143
                     /locus_tag="OO572_003220"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6662121.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            5219..6652
                     /locus_tag="OO572_003221"
     CDS             5219..6652
                     /locus_tag="OO572_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6662122.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6835..9015
                     /locus_tag="OO572_003222"
     CDS             6835..9015
                     /locus_tag="OO572_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6662123.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(9156..10439)
                     /locus_tag="OO572_003223"
     CDS             complement(9156..10439)
                     /locus_tag="OO572_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6662124.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(10574..>11029)
                     /locus_tag="OO572_003224"
     CDS             complement(10574..>11029)
                     /locus_tag="OO572_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662125.1"
                     /translation="AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVD
                     ALGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTF
                     HELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2778 a         2474 c         2745 g         3032 t
ORIGIN      
        1 aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga tctgcttcag
       61 gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc cccccttcag
      121 tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt aaaaacagtc
      181 tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga accgaagttt
      241 gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc tcaatagtta
      301 tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg ttttcaaaat
      361 aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt aatctattgc
      421 tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta agatggttgt
      481 gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc agttcgttgt
      541 tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat aacctattat
      601 actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct aattgatttt
      661 cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt aagaaattat
      721 ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt aatttattac
      781 agctcacatc aagcttttta atgcaagcag ggagtacagg tagcgaggaa agcgccaagg
      841 ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt cttaccgcct
      901 cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg gacaaataat
      961 ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaatg tttctgagca
     1021 tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat ttaatgtatc
     1081 tctcctaatg aggagggtgt ttcagaatat ttgccataag ggaatgagtg ttatatcatg
     1141 ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct ttttttgatg
     1201 ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg agtaagatac
     1261 tacaacatct ccaactattt tagccagcct gactacttga cttggtaata tttgttgcta
     1321 ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat ttagtaatga
     1381 tcaaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat tctattttat
     1441 aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac ttttgacgca
     1501 gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta tcaacaaatg
     1561 accagaatac cgatctacaa cgtaatgcgt ttatttgtgc cggaagtgag ttgattgttg
     1621 cgcgaatggg aagttggcca gaagttgtta tggcatagtg tttacggtcg ttgatacagg
     1681 taaacaaaag cggggagagt catactccct gaatagtcgc gaaaatatct gtggcattgt
     1741 caaattcgtg tcggtgacta atattttagt aatatgccat tcgaaatgga gtgctaactt
     1801 attgatttaa ttgatgtgcg atattgtatt ttatcgtgtt ttgttgtctg ttttttatcc
     1861 agtaagtgtt gttatatgta tgaaagataa gggatttatc atttttgagg tgtgttcatg
     1921 aagcaaacag tttatatcgc cagccctgag agccagcaaa ttcacgtctg gaacctgaat
     1981 catgaaggcg cactgacgct gacacaggtt gtcgatgtgc cggggcaggt gcagccgatg
     2041 gtggtcagcc cggacaaacg ttatctctat gttggtgttc gccctgagtt tcgcgtcctg
     2101 gcgtatcgta ttgccccgga cgatggcgca ctgacctttg ccgcagagtc tgtgctgccg
     2161 ggtagtccga cgcatatttc caccgatcac caggggcagt ttgtctttgt aggttcttac
     2221 aatgcgggta acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg cgtcgtcgat
     2281 gtagtcgagg ggctggacgg ttgccattcc gccaatatct caccggacaa ccgtacgctg
     2341 tgggttccgg cattaaagca ggatcgcatt tgcctgttta cggtcagcga tgatggtcat
     2401 ctggtggcgc aggaccctgc ggaagtgact accgttgaag gggccggccc gcgtcatatg
     2461 gtattccatc caaacgaaca atatgcgtat tgcgtcaatg agttaaacag ctcagtggat
     2521 gtctgggaac tgaaagatcc gcacggcaat atcgaatgtg tccagacgct ggatatgatg
     2581 ccggaaaact tctccgacac ccgttgggcg gctgatattc atatcacccc ggatggtcgc
     2641 catttatacg cctgcgaccg taccgccagt ctgattaccg ttttcagcgt ttcggaagat
     2701 ggcagcgtgt tgagtaaaga aggcttccag ccaacggaaa cccagccgcg cggcttcaat
     2761 gttgattaca gcggcaagta tctgattgcc gctgggcaaa aatctcacca catctcggta
     2821 tacgaaattg ttggcgagca ggggctactg catgaaaaag gccgctatgc ggtcgggcag
     2881 ggaccaatgt gggtggtggt taacgcacac taaccgctga tttaaccggc gcagtctctc
     2941 ctgcgccggt gtattaactt atctcctgta acgcatgtct ctggcgttcg acgatattgg
     3001 tccacaaatt gtctttatcg tcagtccaca aattaatcag caaggaaaaa aagcgttcag
     3061 ctgccggaga aagcacggca tctttgcggc gaataatccc caatgtccga cgtataacgg
     3121 gttccaccag tgggatacca ataatcgaag aatagggcgc atggggcatt gccagaccag
     3181 gaagcgccga aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa agatgacgca
     3241 cttcgtaaaa ccagtccagc ttccagggct tgtcggctag ctgttgttct atcaacaatc
     3301 ggttgccgct ggaggaacgt acgccaatca ttttgtaacc cactaactcc tgccattcta
     3361 caagctactt tttggccaat ggatggtcac gccgacaggc cagcacgaat ggctcgttga
     3421 ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt catgtttatg ccaaagtctg
     3481 attcgttaca cagcaccgat tccatgcaat tattcgtgcc ttgttccaga atccgcactt
     3541 taatattggg atacagctca ttaaatttac cgattgccag cggtaaaaaa taaaatactg
     3601 cggtcggaat acacgctaat gtcaccatac cacgatgata agcattcata tcacgaatat
     3661 taaaaagcgt ttcatcgaat ttctttatta attcccgcgc ctccgggagc aacctttttc
     3721 ctgcttttgt taacgtaacc ttgcgagttg tacgctcaaa tagttgaata tgtaaatctt
     3781 cctccatctt ttttattctt cgcgttaatg caggttgcgt aatattgagt aatttagcgg
     3841 cattgttaaa tgaactggac tctgccagta tgacaaatgc cttcatactt gataattcat
     3901 gcttcattat tactccggaa aatggaagcg acgattttgg ggggctggtc gttaaaaatt
     3961 ttaactgcat ttagccaact taaattaatg aaaaaatgtt attaatcgtt gagctaaagt
     4021 cattagagat gctttgtcct taatgtaacc atatcgcaat aagttatgtt tttaaattga
     4081 gggcattatt atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct cgaggggcgc
     4141 gttcctgtta gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa tattgatggc
     4201 aattatgggt tccggtaacg atctggaaat tgacggtatt ggcggcggta atccactgac
     4261 cagtaaagtc gccattatta gccgttccag cgatccgcgt gctgatgtcg attatctgtt
     4321 tgctcaagtt atcgtccatg agcaacgtgt cgataccacg cctaactgcg gcaatatgct
     4381 gtctggcgta ggggcattcg ccattgaaaa tggtttgatt gcagcgactt cgccagttac
     4441 ccgtgtacgt atccgcaacg tcaatacggg tacgttcatc gaagctgatg tgcaaacgcc
     4501 aaatggtgtt gtcgagtacg agggtagcgc cagaattgac ggcgtaccgg gtactgccgc
     4561 accggttgcg ctcactttcc tgaatgccgc tggaacgaaa actggaaaag ttttcccgac
     4621 tgataatcag attgattatt ttgacgatgt cccggtgacc tgtatcgata tggcgatgcc
     4681 agtcgtcatt attccggctg aatatctggg aaaaacaggt tatgaattac cggcggaact
     4741 ggatgccgac aaagcattat tagcccgcat tgaatctatc cggctacaag cgggtaaagc
     4801 aatgggctta ggtgatgtca gtaatatggt tatccctaaa cctgtgctta tttctccagc
     4861 gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg cattcttgcc atcgcgcgct
     4921 ggcgataacc ggtgctattg ctatttccag tagttgtgca ttggaaggca ccgtcacccg
     4981 acaaatcgtc ccttctgtag gatatggcaa tatcaatatt gaacacccca gtggtgcgct
     5041 cgacgttcat ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat ctgttattcg
     5101 gacaaccaga aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt cgttgtcagg
     5161 ataaggacaa tcaataaagg acttctgtat gagtcataca gaaagaacag gattttaaat
     5221 gaataagaaa tcgttatgga agctaattct gatattagcg atcccatgta ttattggttt
     5281 tatgccagct ccggcaggat taagcgaact ggcgtgggtg ctttttggta tttacctggc
     5341 ggccattgtg ggtctggtta tcaagccttt cccggaacct gtcgtactgt taattgccgt
     5401 tgctgcctca atggtggtgg tcggtaactt atccgacggt gcgtttaaga ccaccgccgt
     5461 attaagcggt tactcttcag gtactacctg gctggtgttc tcggcgttta ccttaagcgc
     5521 cgcatttgtg accaccggtt taggtaaact tattgcctat ctgctgattg gtaaaatcgg
     5581 taacaccatg ctgggtctgg gttacgttac ggtattcctc gatctggtac tggctccggc
     5641 aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca acagcgtggc
     5701 ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt acctgatgat
     5761 gtccatttac atggtcacca aaaccaccag ctatatgttc tttaccgcaa tggcggggaa
     5821 cattctggcg ctgaaaatga tcaacgacat tctgcacctg caaattagct ggggtggatg
     5881 ggcgctggcc gccggattgc cgggcatcat tatgctgctg gtcaccccgc tggtaattta
     5941 caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta aagcgggcct
     6001 tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct ttgtgctggc
     6061 gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg tggcaatcgt
     6121 tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa
     6181 aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct ccttattatc
     6241 gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg catttgatgg
     6301 tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc gttatttctt
     6361 cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc tggcgaacgt
     6421 ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact cctatggcgg
     6481 catggttact cactatggcg gcgcggcagg tccggtcatc tttggcgtgg gttacaacga
     6541 tattaaatcc tggtggttgg tcggtgcggt actgacgata ttaaccttct tggtgcatat
     6601 caccctcggc gtgtggtggt ggaatatgct gatcggctgg aacatgctgt aaatatactc
     6661 gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca cttacttatg
     6721 taagctcctg gggattcact cacttgtcgc cttcctgcaa ctcgaattat ttagagtata
     6781 tccatttatt atctttctgc gcacttcacg gtgcgcagat atctggagca tttgatgatc
     6841 aagttatctg aaaaaggcgt gtttctcgcc agtaataacg aaataattgc cgaagaacat
     6901 ttcaccggcg aaattaaaaa agaagaagca aaaaaaggca ctattgcctg gtctattctc
     6961 tcttctcata atacgtccgg aaatatggat aaacttaaaa ttaagtttga ttcattagcc
     7021 tctcacgata ttacctttgt tggtattgta cagaccgcta aagcgtccgg aatggaacgt
     7081 ttcccgctgc cgtatgtgct gaccaactgc cataactcac tctgcgccgt cggcggcacc
     7141 attaacggtg atgaccatgt ttttggttta tcggcagctc agcgttatgg cggtattttt
     7201 gtgcctccgc atattgcggt catccatcaa tatatgcgtg agatgatggc tggcgggggc
     7261 aaaatgatcc tcgggtcaga cagccacacc cgttacggtg cattagggac aatggcagtc
     7321 ggtgagggtg gcggtgagtt ggtaaaacag ctgcttaatg acacctggga tatcgactat
     7381 ccgggcgtgg ttgcggtgca tctgaccgga aaacctgcgc cgtatgtgga gccacaggat
     7441 gtggcgctgg ctatcattgg cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg
     7501 gagttcgttg gaccgggcgt tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg
     7561 atgaccacgg aaaccacctg tttaagctcc gtctggcaaa ctgatgaaga agtgcataac
     7621 tggctggcgc tgcacggtcg cggtcaggat tactgccagc ttaaccctca accgatggcg
     7681 tactacgatg gctgcatcag cgttgattta agcgccatca aaccaatgat tgcgctgccg
     7741 ttccacccga gcaacgtgta tgaaatcgac acactgaacc agaacctgac cgacattctg
     7801 cgtgagattg aaattgagtc cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg
     7861 gataaagtgg aaaatggtcg cctgaaagtg cagcagggga ttatcgcggg ctgttctggc
     7921 ggtaactacg aaaacgtcat cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat
     7981 gacaccttct cgctggcggt ttacccgtca tcacagccgg tgtttatgga tctcgccaaa
     8041 aaaggtgtgg tagcagattt gattggcgca ggcgcaatca tcagaaccgc attctgtggc
     8101 ccatgctttg gcgcgggcga tacgccaatc aacaacggtt tgagtattcg ccacaccacg
     8161 cgtaacttcc cgaaccgcga aggctctaag ccagccaatg ggcagatgtc agcggtggcg
     8221 ttgatggacg ctcgttctat cgctgcgact gcggcaaacg gtggctattt aacctctgcc
     8281 agcgaacttg attgctggga caacgtgccg gagtacgcct tcgatgtaac gccgtataaa
     8341 aaccgtgttt atcagggctt tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg
     8401 aacattaaag actggccgga attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc
     8461 tcgaagatcc tcgacgaagt caccaccacc gacgaactga ttccttccgg tgaaacctct
     8521 tcttatcgtt caaatccgat tggtctggcg gggaatgtca gcgagctgac agaggtgttt
     8581 gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggt
     8641 agcatggtct atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc
     8701 cagcgtgtga ttggcggtct ggcgaatatt gccgaggagt acgcgactaa acgctaccgt
     8761 tctaacgtca tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc aacctttgaa
     8821 gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc gggtacgacg
     8881 tttaaaggtt atgtgatcca tgaagatgcg ccggtaacgg aaattacgct ttacatggaa
     8941 agcctgacgg ctgaagagcg cgagattatc aaggcgggta gtttgattaa cttcaataaa
     9001 aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact tattgtcgga
     9061 tgcgatgctg gcgcatctta tccgacctac aaatcgcatc gaaccgtagg ccggataagg
     9121 cgtttacgcc gcatccggca aatagttaat agctcttact tcttctcctc tgcaaccact
     9181 ttgctgccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
     9241 ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa cggacgatta
     9301 gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat ggcgctatca
     9361 cggatcacca cctgaccgtt ggtattagca tcaacatcca gcgagcggcc cagttgcgcc
     9421 acgccatcac cggaagcatt gaaacggctg tttacggcga ggaaaccgta atagatgtta
     9481 gacagcgtag ccggtgcaaa cacatatgct tcttgctggg tacgtgagtt caccacgcgg
     9541 aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
     9601 atatagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg gttctgcaca
     9661 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt
     9721 acttggtcac cgtcagtacg cagtgctacc gccggatggt tacctgcatc tacgctatcg
     9781 cccagcgtgt tttcgatggt cagattttgc agttgcagtc cattgttttg tgaccagaag
     9841 accgcagaac acagaacacc gatactgtcg ctgcgtttgc tctggcagct atcgtacata
     9901 taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
     9961 gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
    10021 gtaccgtaca gagtgattcc acctggagca gcagggacat acaccgttcc ctgatactca
    10081 ccaggcatca cggcaatata ctggcgcttg ttggtgcgct tgataattgc cgcatctacc
    10141 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
    10201 ggttgcgcag gcagggttat cggggacgga ttccacgctg cagcacctgg tgtcagggat
    10261 gcaaaatagt gttgcgcatc gaaattcagc gcttcttttg ccgacagaat cgggcgagaa
    10321 gaggtaccag gcgcagtttg atcagaagga cgttgatcgg gtggtgttga gctacaggcg
    10381 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
    10441 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
    10501 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cattatcagt
    10561 caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat
    10621 actgtgatgc catggtgtcc gacttgtgcc cgagaagatg ttgagcaaac ttatcgctta
    10681 tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggtggatccc
    10741 cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg
    10801 atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc
    10861 atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga
    10921 tggcaatttt tacgcctgtt ttgctttgct cgacataaag atatccatct acgatatcag
    10981 accacttcat ttcgcataaa tcaccaactc gttgcccggt aacaacagc
//
LOCUS       DAJTYL010000117        11002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYL010000117 DAJTYL010000000
VERSION     DAJTYL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.117"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            439..2691
                     /locus_tag="OO572_003225"
     CDS             439..2691
                     /locus_tag="OO572_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662126.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3027..3959
                     /gene="csiD"
                     /locus_tag="OO572_003226"
     CDS             3027..3959
                     /gene="csiD"
                     /locus_tag="OO572_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6662127.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3979..5247
                     /gene="lhgO"
                     /locus_tag="OO572_003227"
     CDS             3979..5247
                     /gene="lhgO"
                     /locus_tag="OO572_003227"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6662128.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5270..6718
                     /gene="gabD"
                     /locus_tag="OO572_003228"
                     /pseudo
     CDS             5270..6718
                     /gene="gabD"
                     /locus_tag="OO572_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6726..8012
                     /gene="gabT"
                     /locus_tag="OO572_003229"
                     /pseudo
     CDS             6726..8012
                     /gene="gabT"
                     /locus_tag="OO572_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8250..9650
                     /gene="gabP"
                     /locus_tag="OO572_003230"
     CDS             8250..9650
                     /gene="gabP"
                     /locus_tag="OO572_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6662129.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9671..10333
                     /gene="csiR"
                     /locus_tag="OO572_003231"
     CDS             9671..10333
                     /gene="csiR"
                     /locus_tag="OO572_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6662130.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10334..10783)
                     /gene="kbp"
                     /locus_tag="OO572_003232"
     CDS             complement(10334..10783)
                     /gene="kbp"
                     /locus_tag="OO572_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6662131.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10867..>11002)
                     /locus_tag="OO572_003233"
     CDS             complement(10867..>11002)
                     /locus_tag="OO572_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6662132.1"
                     /translation="TIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQTRD
                     "
BASE COUNT         2808 a         2706 c         2779 g         2709 t
ORIGIN      
        1 ccgacaccgc ctccattcgc aaagtcgata ctcgcagtca acaagcaaat gttaataatt
       61 agcactatct atagttatca tcgattcaat gatactttgt aatgattttg tatctaataa
      121 tataacttta ttacattagc tgaagagttt tcgcatcatt atgatatctg ttacttttca
      181 ctccataaaa ataaacttca tatagcaata tattctttca tagatcttat tctgctaatc
      241 attagtttcg tatgcgcgat ttttgacagt tgcctctcca gaccacatcg ataattaaaa
      301 aacagattta aacattatcc ttttccatat agatattgga taaaaatagg acatctgttt
      361 gcaattactt tcacaacaat taaacatttt tatgttttcg tatacatcat attactctac
      421 cattagagga acgttattat gttttctata aaaccaggac ctggaaattt acctatcgac
      481 aaccccacat tgttatcatg gaacattact gatggggatc taaattccaa atttaataca
      541 ttagaatatc taaactgtat aacaaatatt attaatgctt gtggagttta tcctcaggat
      601 ttaaaagaca gagaaattat atcaactttt cacgcagaaa aagtcattaa tgatctgtta
      661 aaaaacgatt ataaaatttc cctttctcca gatacaactt accgagagct gaataaagct
      721 gcacagagta gcattacagc gccagacagg ataggagaag ggaaaacatg ggtttatcaa
      781 cgagatacaa tggttgaaag aggtgataac agtggtgttc atcagtatgg tccagctgaa
      841 catttcactc acattatatc tgacaaaccg tccccaaaag ataagtatgt tgcatatgct
      901 attaacattc ctgactatga gctagcagcc gatgtatata atattaacgt gacgtcacct
      961 tccggacagc aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt
     1021 gagcgtaaat ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa
     1081 cctggcgaag cgattcttca tgcttttaat gccacctacc agcaaatcag ggaaaatatg
     1141 tctgagtttg cacgttccca ttatgggtat atacaaatcc ctccagtgac aactttccgc
     1201 gccgacggac cagaaactcc cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc
     1261 gaagatcttt gtaccatcca taatccaatg ggggatttgc aggattttat cgcattggtt
     1321 aaagatgcta aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga
     1381 atcgggggaa gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc
     1441 agtaaagata tagaaagtgg tatccctggc tattggcaag gtcaggtttt gattccattc
     1501 attaaagatc cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact
     1561 gcaaaagact tcgaagcaag taaagataac atctctaagg atgaatggaa aaacctccat
     1621 gcattaaaag aaaagagttt aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt
     1681 attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt
     1741 tatgatgcag caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt
     1801 aaaaattata atgagcgatt acacaatact aacttattta acccaaaata tcatgaaaaa
     1861 accgttatga attacatgga atatctggta acttgtcagt tgaatgaaga acaaatgtca
     1921 tcgctgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct catgaagacg
     1981 ataaaagcct tttcattcgg gggagatctc ctaacccttg catcaaaacc gggttccaca
     2041 atctcaagca tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac
     2101 aatggcaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg
     2161 gcatatatgg ccgctctccc gttcagcagg cctttagttt actgggatgg ccaggtatta
     2221 aaatcaacga ctgaaattaa aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg
     2281 cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata
     2341 gataaagcag gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc
     2401 tttagtcgtg gggattctgt gaacattaat cactctcctc atgatggact agttataata
     2461 aataaaggaa acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatatat
     2521 aaaaacatgg ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc
     2581 ccttgtaaag tatttacgct tagaggcgga tctctaaata tcaatattcc taggcgtagc
     2641 gctcttcttt tagggaaaac aggagaaccg ccgaactatc tctatttata atttaatgtt
     2701 atatctgccc cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgta
     2761 gtgaagccat ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc
     2821 taacctgttg ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa
     2881 tgttactaat ttgttgcttt tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat
     2941 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc
     3001 tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg
     3061 tcgattcagg ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc
     3121 tgctggaact caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat
     3181 ggcccgtgca ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg
     3241 acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg
     3301 aaggtgcgct gttgatcaat gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg
     3361 tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg
     3421 gtcagtatta cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc
     3481 agccgcaccg cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt
     3541 acgtgctgat gatgaaaatc gatgactggg aacatctgga ccactatttc cgccatccac
     3601 tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt
     3661 tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt
     3721 tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg
     3781 aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca
     3841 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     3901 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     3961 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     4021 tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa
     4081 gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc
     4141 tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa
     4201 gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacg
     4261 tctcagctcg aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc
     4321 gagcgcgagt ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat caccgggctt
     4381 ggtggcattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg
     4441 gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc
     4501 agtgagcata aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg
     4561 acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa
     4621 ccgggcttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac
     4681 cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg
     4741 catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc
     4801 aaacgcgaag gctatcgcaa gcgcgacttc tcatttagtg acacgctgga gattttgagc
     4861 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     4921 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     4981 ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcagtatc gccggacggc
     5041 aagctgattg acgattttct gtttgtcacc accccacgca cgatccacac ctgcaatgcg
     5101 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     5161 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat
     5221 gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa
     5281 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     5341 tggtgaagtc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     5401 aatgggcgca gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg
     5461 gcgtgcgctc accgccaaag aacgcgccac cattctgcgc aactggttca atttgatgat
     5521 gcagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga
     5581 agcgaaaggc gaaatcagct acgccgcctc cttcattgag tggtttgctg aagaaggcaa
     5641 acgtatttat ggcgacacca ttcctggtca tcaggccgat aagcgcctga ttgttatcaa
     5701 gcagccgatt ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg cgatgattac
     5761 ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca
     5821 gacgccgttc tctgcgctgg cgctggcgga actggcgatt cgcgcgggca ttccggctgg
     5881 cgtgtttaac gtggtcaccg gttcggcagg cgtggtcggt aatgaactga ccagcaaccc
     5941 gctggtacgc aaactgtcgt ttaccggtta gaccgagatt ggccgccagt taatggaaca
     6001 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt
     6061 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa
     6121 cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg
     6181 ctttgccgaa aaattacagc aggcggggag caaactgcac atcggcgacg ggctggagaa
     6241 aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat
     6301 tgccgatgcg ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg
     6361 cggcaacttc ttccagccaa ccattctggt ggacgttccg gccaacgcca aagtgtcgaa
     6421 agaagagacg ttcggccccc tcgccccact gttccgtttt aaagatgaag ccgatgtgat
     6481 tgcacaagcc aatgacaccg agtttggtct tgccgcctat ttctacgccc gtgatttaag
     6541 ccgcgtcttc cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca ataccggcat
     6601 tatttccaat gaagtggccc cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg
     6661 ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact
     6721 ggagaatgcg aaggaacagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc
     6781 gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg
     6841 acgtttaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc
     6901 acctgcatcc gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct
     6961 gcttccaggt gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg
     7021 tgccgggcga tttcgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa
     7081 acgcggtaaa aatcgcccgc gccgccacca aacgtagcgg caccatcgct tttagcggcg
     7141 agtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg
     7201 cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg
     7261 gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat gatgccgcgc
     7321 cggaagatat cgccgccatc atgattgagc cggttcaggg cgaaggcggt ttctacgccg
     7381 cgtcgccagc ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg atcatgctga
     7441 ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga
     7501 tgggcgtggc gccggatctt accacctttg cgaaatcgat cgcaggcggt ttcccgctgg
     7561 cgggcgtcac cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt ctgggcggca
     7621 cctatgccgg taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc
     7681 aggaaaatct gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac ggattgttgg
     7741 cgatagccga aaaacacccg gagatcggcg acgtacgcgg gctgggatcg atgattgcca
     7801 tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg
     7861 tggcacgcgc ccgcgataag ggcctgattc ttctctcctg cggcccgtat tacaacgtgc
     7921 tgcgcatcct tgtaccactc accattgaag acgctcagat ccgtcagggt ctggagatta
     7981 tcggtcagtg ttttgatgag gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg
     8041 cgcgtcttgt ccggcctacg agaatccaag tcggataagg cgtttacgcc gcatccgaca
     8101 atctgtacgt gaacaggaat aaatctatgt cggccggata aggcggagcc gctctccggc
     8161 aaaaagaatc aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc
     8221 caagtgacac actttcgaga ggattcagga tggggcaatc atcgcaacca cacgagttag
     8281 gcggcgggct gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa
     8341 gtctgtttgt cggttccagc gtcgccatcg ccgaagcgga cccggcggta ttactggcct
     8401 atctgttcgc cggattactg gtggttatga ttatgcggat gctggcggaa atggcagttg
     8461 ccacgcccga taccggttcg ttttccacct atgccgataa agccattggc cgctgggcgg
     8521 gctataccat cggctggttg tactggtggt tttgggtact ggttatccca ctggaagcca
     8581 acatcgccgc catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc
     8641 tcgtcattac cctcgcctta actggcagta atttattaag cgttaaaaac tacggcgaat
     8701 ttgagttctg gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg
     8761 cagtcgcaat tagcggtttt tacccttatg ccgaagtgag cgggatctca agattgtggg
     8821 atagcggcgg ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca
     8881 tgttctcgtt tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacatgccgg
     8941 aaaaacatat tgtccgcgcc accaactcgg ttatctggcg tatttctatc ttctatttgt
     9001 gctctatttt tgtcgtcgtg gcattaatac cgtggaatat gccggggctg aaagccgttg
     9061 gatcttatcg ctcggttctg gaattgctca atattcccca tgcgaaatta atcatggact
     9121 gtgtgatatt actttccgta accagttgcc tgaactcggc cctgtatacc gcctcaagga
     9181 tgctctactc cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca
     9241 gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc agcattctta acggtggtgg
     9301 tgaactatta cgcgcctgcg aaggtattta aatttctgat cgacagctcc ggcgctatcg
     9361 ccctgctggt ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa attctgcgag
     9421 cagaaggaag cgaaattcgc ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca
     9481 tcggctttat tacctttgtg ttggtagtga tgctattccg tccggcgcaa cagttagaag
     9541 tgatctccac cggcttatta gcgataggga ttatctgtac cgtgccaatt atggctcgct
     9601 ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag
     9661 gagtcacacc atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat
     9721 tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt ttactgacat cgcgttatgc
     9781 acttggcgtt gggccgttgc aggaagcact ttctcaactg gtggcggaac ggctggtcac
     9841 ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat
     9901 tttcgacgcc cgcgccaata tggaagcgat gctggtcagt ctggcgattg cccgcggtgg
     9961 cgatgaatgg gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg
    10021 tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc
    10081 aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct
    10141 ggcggcgcgt tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga
    10201 ggataaacac gatcagcacc agaccctgac tgcggcggta ctgccgcgag atacagcgcg
    10261 cgccagtgag ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat
    10321 ggctggcaat taactactct tccggaatac gcaacacttg ccccggataa attttatccg
    10381 ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt
    10441 agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg
    10501 cagtggctgg tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc
    10561 ccaccgcaac aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg
    10621 ctttgccgtc ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca
    10681 gatgctcctg caccttcttc gcctgatcgt ctttatcgtg ctgacctgta accgcatccc
    10741 agagtttttc tccggcatct ttaacaaaat tgaacagacc catagctacc tcattcgtta
    10801 acggaaacac tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc gggggtgagc
    10861 acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag
    10921 cagtgtcagc agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc
    10981 gatcggcggc agaatgatgg tg
//
LOCUS       DAJTYL010000118        10968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYL010000118 DAJTYL010000000
VERSION     DAJTYL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.118"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..393)
                     /locus_tag="OO572_003234"
     CDS             complement(<1..393)
                     /locus_tag="OO572_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6662133.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAP"
     gene            complement(505..1533)
                     /gene="malI"
                     /locus_tag="OO572_003235"
     CDS             complement(505..1533)
                     /gene="malI"
                     /locus_tag="OO572_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6662134.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1708..3300
                     /gene="malX"
                     /locus_tag="OO572_003236"
     CDS             1708..3300
                     /gene="malX"
                     /locus_tag="OO572_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6662135.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3310..4482
                     /gene="malY"
                     /locus_tag="OO572_003237"
     CDS             3310..4482
                     /gene="malY"
                     /locus_tag="OO572_003237"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6662136.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4586..5587
                     /gene="add"
                     /locus_tag="OO572_003238"
     CDS             4586..5587
                     /gene="add"
                     /locus_tag="OO572_003238"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6662137.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5623..6663)
                     /locus_tag="OO572_003239"
     CDS             complement(5623..6663)
                     /locus_tag="OO572_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6662138.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6906..7031
                     /gene="blr"
                     /locus_tag="OO572_003240"
     CDS             6906..7031
                     /gene="blr"
                     /locus_tag="OO572_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6662139.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7304..7519
                     /gene="ydgT"
                     /locus_tag="OO572_003241"
     CDS             7304..7519
                     /gene="ydgT"
                     /locus_tag="OO572_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6662140.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7605..8045
                     /locus_tag="OO572_003242"
     CDS             7605..8045
                     /locus_tag="OO572_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6662141.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8122..8703
                     /gene="rsxA"
                     /locus_tag="OO572_003243"
     CDS             8122..8703
                     /gene="rsxA"
                     /locus_tag="OO572_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6662142.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8703..9281
                     /gene="rsxB"
                     /locus_tag="OO572_003244"
     CDS             8703..9281
                     /gene="rsxB"
                     /locus_tag="OO572_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6662143.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9274..>10968
                     /gene="rsxC"
                     /locus_tag="OO572_003245"
     CDS             9274..>10968
                     /gene="rsxC"
                     /locus_tag="OO572_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6662144.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2545 a         2828 c         2775 g         2820 t
ORIGIN      
        1 tggcgcaaca agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg
       61 aaaatctgcc attggcatat caaacggttt aggaccaccg ccaccggcgt tgttaaccag
      121 gatatcaact ttacccagat tactgacggc aaagtctgcc agtgcagaga gttcctgttc
      181 ggaagtaata tcacaacgcc aggcaaatgc ctgaccatcc agttgttgaa tttcgtctac
      241 aacatggtta gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc
      301 gaatgtaatg gcgatttctt taccaatacc tgcacccgca cctgtgatga tggcgcattt
      361 tccgtcgagt ctcaggttgt cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa
      421 ataaaaggca ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac
      481 caagtatagc agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag
      541 attgcgtgaa tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtgat
      601 accaagttcc cgcgctggcg tgctggccca ggtaacaggt atatcatcaa gtgtggttgg
      661 tgtcgcatcg gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct
      721 ttccccgctt tgccgccctg ctttcagcaa accaaaccat gcccccatcg caatagtttc
      781 gttatagcaa accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc
      841 cgcggcttgc ttctggctgg aagtgcactc caacacccaa tcgctgtgaa acggcaggcc
      901 aaattttagt agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact
      961 ttgccctccc agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa
     1021 ctgtgcagcc tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg
     1081 ggaagcgaaa atcaccggga tagctttttc ttctgccatc cgccgcaggt catcgctgct
     1141 tcctgcagcc ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg
     1201 ctgtgccagc tgctcgccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgtgc
     1261 ttccagagct tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg
     1321 gacgatcaaa ccaatgacgc cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg
     1381 cacaaatccc agctcttcaa tggcggcgtt aacgcgttct cctgtggcgg tagagattcg
     1441 ccctttgcca ttaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac
     1501 atcatgaatg gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg
     1561 acagataaaa cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg
     1621 atttatgact cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc
     1681 agattgttct ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg
     1741 tgggagttct tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc
     1801 tgcggcatta tgctcggcat tggtagttct cttagcagcc atgatgtcat aaccctgatc
     1861 ccggtcctgg gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg
     1921 tttgctttta gtttcctgcc tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc
     1981 gaaaataaag gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg
     2041 gtaaacttct ggttgaccaa taaaggcatt ctgccaacca cagatgccgc cgttctgaaa
     2101 gccaataaca tccagagcat tcttgggatc cagtcgatcg ataccgggat cctcggtgcg
     2161 gtgatcgccg gtattatcgt ctggatgctg catgagcgtt tccataatat ccgcctgccg
     2221 gatgcgctgg cattcttcgg cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg
     2281 ggccttgtcg gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc
     2341 ttgggccata tgataaacag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa
     2401 cgtctgctgt tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac
     2461 gcaggcggca cgcaggaggt ctgcggtcaa accgtcagcg gcgcactgac catcttccag
     2521 gcgcaattga gttgcccgac cactcacggt ttttctgaaa gcgtcacgcg tttcctttcg
     2581 caaggtaaaa tgcctgcgtt tctcggcggt ctaccaggtg cagcgttagc tatgtatcac
     2641 tgcgcgcgcc cggaaaatcg ccataaaatt aaaggtctgc tgatttctgg cctgatcgcc
     2701 tgcgtcgttg gcggcactac cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt
     2761 ctgtatgtca tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc
     2821 gtcaccatcg gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat
     2881 ggtctgtcaa ccaagtggta catggtgcca gtggtggcgg caatctggtt tgtcgtttac
     2941 tacgtcatct tccgtttcgc tatcacccgc ttcaatctga aaaccccggg gcgcgatagc
     3001 gaagttgcca gctcaatcga aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac
     3061 gttcctgcaa tcctcgaagc attaggcggt gccgacaata ttgtcagcct cgataactgc
     3121 attacccgtc tgcgtttgtc tgtggaagat atgtcgcttg ttaatgtgca ggcactgaag
     3181 gacaatcggg caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg
     3241 ccacaagttc agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa
     3301 ggataagata tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag
     3361 tgggattatg tcgctgaccg tttcggcact gctgacctgt taccgttcac gatttcagac
     3421 atggattttg ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc
     3481 gtatttggct acagccgctg gaaaaacgat gagtttctcg cggctattgc ccactggttt
     3541 tccacccagc attacaccgc aattgatacg cagacggtgg tgtatggccc ttctgtcatc
     3601 tatatggttt cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac
     3661 acgcccgcct atgacgcatt ttacaaggcc attgaaggta accagcgcac agtaatgccc
     3721 gttgctttag agaagcaggc tgacggctgg ttttgcgata tgggcaagct ggaagccgtt
     3781 ttggcgaaac cagaatgtaa aattatgctc ctgtgtaacc cacagaatcc taccgggaaa
     3841 gtgtggacgt gcgatgagct ggagatcatg gctgacctgt gcgagcgtca tgatgtgcgg
     3901 gttatttccg atgaaatcca tatggatatg gtctggggcg agcagccgca tattccctgg
     3961 agtaatgtgg cgcggggaga ctgggcgttg ctgacatcgg gatcgaaaag tttcaatatt
     4021 cccgcgctga ccggtgctta cgggattata gaaaatagca gcagccgtga tgcctattta
     4081 tcggcactga aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat
     4141 atcgccgcct atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat
     4201 aacctgacgt atatctcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc
     4261 ccgcaatcca cttatctggc ctggcttgat ctacgtccgt tgaatattga cgacaacgcg
     4321 ttgcaaaaag cgcttatcga gcaagaaaaa gtcgcgatca tgccggggta tacctatggt
     4381 gaagaaggtc gtggttttgt ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa
     4441 ggtgtggctg gattaataaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta
     4501 aaaaatcgtt gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt
     4561 ttatatccag aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca
     4621 tcgccacctt gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa
     4681 tatctcgctt cctgcacaat cccttgaaac actgattccc cacgttcagg tcattgccaa
     4741 cgaacccgat ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc
     4801 tcttgatgcc tgccgccgcg tggcatttga aaacattgaa gatgcagccc gtaacggcct
     4861 gcgctatgtc gagctgcgtt tttcaccagg ctacatggca atggcacata agctgcctgt
     4921 agcgggtgtt gtcgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt
     4981 gcaggcgaag cttatcggca ttatgagccg aaccttcggc gaagccgcct gtcagcaaga
     5041 gctggaggcc tttttagccc accgtgacca gattaccgca cttgatttag ctggtgatga
     5101 acttggattc ccgggaagtc tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg
     5161 gcatattacc gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg
     5221 tgaactgggt gcggagcgta ttggacatgg cgtaaaagcc attgaagatc gggcgctgat
     5281 ggattttctc gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac
     5341 cagcaccgta gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg
     5401 tgccagcatt aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac
     5461 cgttgccgcg ccagctgctg ggttatcccg tgagcaaatc cgccaggcac agattaatgg
     5521 tctggaaatg gctttcctca gcgcagagga aaaacgcgca ctgcgagaaa aagtcgccgc
     5581 gaagtaacaa aaatggatgg tgcaaattgc accatccatt tttcacgcaa ggcacaacgt
     5641 cgcgcgatgt ttggcggatt cgatgcccag ctcaatcaac tccattacct ggattgcctg
     5701 gcttgccgga accggatttt caccatcgcc atttaacgca tcacgaatcg ccgcatagta
     5761 agccggataa ttcccaggca ccgtcaacag cgtttcttcg acacgttcct caccttccac
     5821 gcgggtaagt acgccatcac gcatatcgta gccccagtct tcctgcggca gacgctcgcc
     5881 atttttcaga cgttcttcct gtggatcgag gccatatttc acataactgc ctcgggatcc
     5941 atgcacgata taccgtgctg actcagcggc tgccagcatg gtaccgtgta aaatgactcg
     6001 ccgctgtgga taggacaaga tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa
     6061 ctgtgccaaa tctaccgtca tgctgaccgg taaaccaaat agcgtaatgg cctgatcaag
     6121 aagatgcggt gctaaatcgt accagatacc gctgcctggg ccgccctgtt cacgccaacg
     6181 atcgcgcacc tgcggacgga agcggtcaaa atgagactca aagtaagcaa cttcacccag
     6241 cacgccttcc gcgagcaaac cttttagcgt caagaaatcg ctatcccaac ggcggttatg
     6301 gaatacagac agcacacgcc ccaggctttt tgccagcgca tccagctctc gcgcttgtga
     6361 cagtgtcacg gtaaagggtt tatcaacgac cacatgttta cccgcctcaa gcgccgcttt
     6421 ggctaacggg aaatgggtat cgttgggtgt aggaatgaca atcaggtcta tgttgggatc
     6481 gttaaacaga tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg cttttacttt
     6541 tgtttcatca ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg caatcagggg
     6601 cgcatggaag gttttgctcg cataaccata cccaatcaac ccaacacgga tgttgtcgct
     6661 catgtcattg cctctcattg aagtatgatg gctatttgac actatccttt acccacgctc
     6721 aacagtttaa taacctgcca gcaataaggg atgttgttta acttaagtca aaaaaatagc
     6781 gaattttcca acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc
     6841 tattattgct aagtaacatt taccccctga agttaatgga tcaatcaaga gagatgtggg
     6901 ctgtaatgaa tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt tcagtcattc
     6961 ttcttggcgt ggcgagtcac attgacaact atcagccacc tgaacagagt gcttcggtac
     7021 aacacaagta agctctgcac ttgtggagcg acatgctgcc cgtccgggtg catgttttca
     7081 cttgtcggat attaaaccag gaatttatta tcttgttcga tgttgttggt gattgtcagg
     7141 gatagtaaag cgcgttactc ttttcagagc agtttttcat ccctgaatat ttgccatcag
     7201 cgcattctta ttattgtaat aacacaagcg ttcacttttg gtgaaatttt atcatcacta
     7261 ataataccta cccctttggt attaattatt ttatggacct tttatgactg ttcaggacta
     7321 cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct
     7381 taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg
     7441 tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt
     7501 ctggcactat gtccaataaa gaaagtagcg atcggttata aaagccttat aataacgccc
     7561 ctaaaattag atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc
     7621 cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttga
     7681 gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa
     7741 cgctcggcgg acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta
     7801 tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg
     7861 ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcagta aaagcattcg
     7921 ctttttcacc cgtggaagtt ggcatagccg ttcgccagtt gctgtttact ttgttggcaa
     7981 ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc
     8041 cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat aataggcggc
     8101 ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg
     8161 gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa
     8221 aaactggaaa ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctgacgtct
     8281 atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc
     8341 accctggcat ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc
     8401 aaaaccagcc cggtgcttta ccgtctgctg gggatttttt tgccgcttat caccaccaac
     8461 tgtgcagtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg
     8521 gcgctgtacg gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc
     8581 atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg
     8641 ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg
     8701 taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg
     8761 ccattctggg ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa
     8821 ttgacgaaat cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct
     8881 acgcggaagc catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag
     8941 ctgtgatgct aaaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag
     9001 cgcaagagct aacgcctgcg cggatggtgg cggttattga tgaaaataac tgtattggct
     9061 gcactaaatg tattcaggct tgtccggtag acgccatcgt tggcgctacc cgtgtcatgc
     9121 atacggtaat gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc
     9181 actgcatctc gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca
     9241 ccattcccgt gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga
     9301 aaaaataaaa tctgggattt caacggcggc atccatccac cggagatgaa aacccagacc
     9361 aacggtaccc ccctgcgcca ggtactactg gcgcagcatt ttgttattcc actgaaacag
     9421 catattggcg ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag
     9481 ccgcttaccc gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt
     9541 acggctattg cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt
     9601 attgatgccg atggtgaaga ctgctggatc ccgcgcgacg gctgggtcga ttatcgctct
     9661 cgcagtcgcg aagagttaat cgagcgcata catcagtttg gtgttgccgg gctgggcggt
     9721 gccggattcc cgacaggcgt taaattgcag ggtggtggag ataagattga aacgttgatt
     9781 atcaacgctg ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgc
     9841 gcggctcagg tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt
     9901 cttatcggca ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg
     9961 gactctaacg atatttctct gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa
    10021 caattaacct acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc
    10081 ggcgtattaa tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc
    10141 gagccgatta ccgagcgtgt tgtaaccctg actggcgaag caatcgctcg cccgggcaac
    10201 gtctgggcac ggctggggac gccagtgcgt catttattga atgatgccgg attctgcccc
    10261 tctgccgatc aaatggtgat tatgggtggc ccgctaatgg gctttacctt gccatggctg
    10321 gatgtcccgg tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgaacttggc
    10381 gaaccacagg aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgtg
    10441 gatcttttgc cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc
    10501 acgcataaca ttgccgactg cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat
    10561 attcccttgg tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa
    10621 gaaaagcgcg ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc
    10681 gaaaaagcga ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat
    10741 aaagatgcga ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag
    10801 cctattgtga ttaaagcggg cgaacgcccg gataacagtg caattattgc agaacgggaa
    10861 gcccgtaaag cgcaagccag agcgaaacag gcagaactgg agcaaactaa cgacgcagca
    10921 accgttgctg aaccacgtaa aactgccgtt gaagcagcta tcgcccgc
//
LOCUS       DAJTYL010000119        10731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYL010000119 DAJTYL010000000
VERSION     DAJTYL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.119"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO572_003246"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO572_003246"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6662145.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO572_003247"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO572_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6662146.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="OO572_003248"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="OO572_003248"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6662147.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="OO572_003249"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="OO572_003249"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6662148.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="OO572_003250"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="OO572_003250"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6662149.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="OO572_003251"
                     /pseudo
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="OO572_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="OO572_003252"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="OO572_003252"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6662150.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         2677 a         2770 c         2710 g         2574 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa g
//
LOCUS       DAJTYL010000120        10601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYL010000120 DAJTYL010000000
VERSION     DAJTYL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.120"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1543
                     /gene="trg"
                     /locus_tag="OO572_003253"
     CDS             <1..1543
                     /gene="trg"
                     /locus_tag="OO572_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6662151.1"
                     /translation="LSSALAGYFLWQADRDQRDVTAEIEIRTGLANSSDFLRSARINM
                     IQAGAASRIAEMEAMKRNIAQAESEIKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQ
                     AYITGMQPMLKYAKNGMFEAIINHESEQIRPLDNAYTDILNKAVKIRSTRANQLAELA
                     HQRTRLGGMFMIGAFVLALVMTLITFMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEP
                     AGRNEIGRLSRHLQQMQHSLGMTVGTVRQGAEEIYRGTSEISAGNADLSSRTEEQAAA
                     IEQTAASMEQLTATVKQNADNAHHASKLAQEASIKASDGGQTVSGVVKTMGAISTSSK
                     KISEITAVINSIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLASRSAQAAKEI
                     EGLISESVRLIDLGSDEVATAGKTMSTIVDAVASVTHIMQEIAAASDEQSRGITQVSQ
                     AISEMDKVTQQNASLVEEASAAAVSLEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPV
                     SFATV"
     gene            complement(1581..2504)
                     /locus_tag="OO572_003254"
     CDS             complement(1581..2504)
                     /locus_tag="OO572_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6662152.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            2721..4064
                     /locus_tag="OO572_003255"
     CDS             2721..4064
                     /locus_tag="OO572_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6662153.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4289..5944
                     /gene="opgD"
                     /locus_tag="OO572_003256"
                     /gene_synonym="mdoD"
     CDS             4289..5944
                     /gene="opgD"
                     /locus_tag="OO572_003256"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6662154.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6084..6308
                     /locus_tag="OO572_003257"
     CDS             6084..6308
                     /locus_tag="OO572_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6662155.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6371..6907
                     /gene="rimL"
                     /locus_tag="OO572_003258"
     CDS             6371..6907
                     /gene="rimL"
                     /locus_tag="OO572_003258"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6662156.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(6902..7882)
                     /locus_tag="OO572_003259"
     CDS             complement(6902..7882)
                     /locus_tag="OO572_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662157.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8006..8998
                     /gene="tehA"
                     /locus_tag="OO572_003260"
     CDS             8006..8998
                     /gene="tehA"
                     /locus_tag="OO572_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6662158.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            8995..9588
                     /gene="tehB"
                     /locus_tag="OO572_003261"
     CDS             8995..9588
                     /gene="tehB"
                     /locus_tag="OO572_003261"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6662159.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            9891..10547
                     /locus_tag="OO572_003262"
     CDS             9891..10547
                     /locus_tag="OO572_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6662160.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
BASE COUNT         2743 a         2485 c         2684 g         2689 t
ORIGIN      
        1 attaagttct gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt
       61 tactgcggag attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc
      121 ccggatcaat atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa
      181 gcgaaatatt gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta
      241 tcagaatcga ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg
      301 ctttcaggct tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt
      361 tgaagcgatt atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga
      421 tattttgaac aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc
      481 ccatcagcgc acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt
      541 catgacgctg ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc
      601 cgcacaacgg attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg
      661 tcgtaatgaa atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat
      721 gacagtaggg actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc
      781 agctggcaat gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac
      841 tgccgccagc atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca
      901 tgccagcaaa ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc
      961 cggtgtagta aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac
     1021 cgccgtcatc aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga
     1081 agccgcgcga gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac
     1141 actcgcaagc cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt
     1201 caggttaatt gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat
     1261 tgttgatgcc gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga
     1321 acaaagtaga ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca
     1381 acagaatgct tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc
     1441 tgcacgatta actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga
     1501 acctcgcgga gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga
     1561 tcgacagatc ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac
     1621 tggcaaccag aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca
     1681 ccatgtcccg gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg
     1741 ccagaaggga caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta
     1801 gacagcgttt cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct
     1861 gaatgctggc gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg
     1921 cttagcgtta cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat
     1981 tcaagaaaca gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca
     2041 atgccgatat cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc
     2101 gcaacttgca aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat
     2161 atccccagtg ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct
     2221 ttacgatgaa gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt
     2281 aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga
     2341 gcgcccgtca gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta
     2401 agggtttcag ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg
     2461 tggcgcaaac ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac
     2521 gaagcggtgt cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta
     2581 ttaacaatta aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag
     2641 atcacagttt gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact
     2701 gaccactgga ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt
     2761 tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg
     2821 aactggtagc tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa
     2881 tggtaaatgc agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg
     2941 ggactgcaca agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg
     3001 tgagctatta cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca
     3061 ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg
     3121 agcttatcga gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata
     3181 tcttcacccc acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg
     3241 aaacacaggc acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag
     3301 caacggtaga tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg
     3361 tggtctgttt tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc
     3421 gggtgcagtc gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc
     3481 cgcgccgcga ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg
     3541 tgttgtttgc ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc
     3601 gtggcgtggc attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg
     3661 gaaccgggca ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc
     3721 ctgacagtga gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc
     3781 cttcgggtga ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg
     3841 aacgtggttg ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg
     3901 cggggatctt ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca
     3961 gtcgcgaagc atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc
     4021 aggaagcgga agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc
     4081 tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa
     4141 atgccgattt atgcatattc tctcagttca acaattggat tattaataaa tattgtctag
     4201 agtgagcggt cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc
     4261 acaacaccag aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat
     4321 ggctatggcc gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt
     4381 cgcggcagat tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca
     4441 gtcaatggcg cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga
     4501 cacgctggcg acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc
     4561 gctctggcat aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg
     4621 attccgtcgc cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat
     4681 tcactttcgc ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga
     4741 agggcaaagc gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg
     4801 ccgtgatgta gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca
     4861 atacggtttg tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt
     4921 ccccgacttt accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt
     4981 ttatgcgttg ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga
     5041 gaaaagtcag gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca
     5101 gctgggcatt tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg
     5161 cgatacaatt catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg
     5221 cgagtggatt tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga
     5281 caacaacccg aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga
     5341 cattatgggc tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg
     5401 taagggcacc atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt
     5461 ctgcttctgg cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg
     5521 tctgtactgg agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg
     5581 taccggcatg ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg
     5641 ggcgcgtcgt tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg
     5701 cattgagccg gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat
     5761 tgaacccatc gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga
     5821 tccggtcgat atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct
     5881 gtatcagtat ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag
     5941 ttaatcgttt tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg
     6001 ctcgcatttt tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg
     6061 tcagccacta acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga
     6121 aaaacgaaaa tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa
     6181 ttgccagaaa ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa
     6241 aacaaaagct acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag
     6301 aggtgtaaac tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac
     6361 tggagataag atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc
     6421 ggaaagtcac gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca
     6481 gtcgctaaac tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg
     6541 taatgtgatg ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga
     6601 acttatcggc gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg
     6661 ctactggctg gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt
     6721 gattcatcat tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga
     6781 caatccgcaa agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa
     6841 acaggctgag ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga
     6901 ttcataataa ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg
     6961 gaccacggac atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact
     7021 ccacatggtt ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt
     7081 gctcgtcgag cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat
     7141 gttttaacac acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa
     7201 tcgtcggaat ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag
     7261 cgcaatcgca tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa
     7321 cttcggcgcg atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct
     7381 ggtggacaat acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat
     7441 ggtcgggcgt taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac
     7501 caaatattct acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat
     7561 atgcaccctg gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc
     7621 cccacagcac gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg
     7681 caatagcatt ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat
     7741 cgatccagcc gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct
     7801 gccgtaataa cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt
     7861 cacttaggcg atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa
     7921 attctgcggc tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta
     7981 taaattaaac aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg
     8041 ctactttggt attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca
     8101 ggtttggcag gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg
     8161 gggattattg actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc
     8221 ggaagttcgc catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct
     8281 ggtggcgatt ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg
     8341 tgtcgtggtt cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca
     8401 ccctgaagaa gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag
     8461 cgcaatggcc tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg
     8521 cgttttctca tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga
     8581 attacccacg gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg
     8641 tagtgcctgg ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg
     8701 ttatggactg ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc
     8761 atttaatgct tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt
     8821 gcatctgggg agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat
     8881 ctttaccaat tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg
     8941 aaaattgtta gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc
     9001 attcgtgacg aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta
     9061 ctggaagcgg tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt
     9121 cgtaacagtc tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc
     9181 atgagtatcg ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc
     9241 cgggtcgttg atctgaataa cctcacattt gatggacagt acgattttat tctttcgact
     9301 gtggtgctga tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt
     9361 tgcactaaac ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca
     9421 tgtaccgtcg gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc
     9481 tgggagaggg tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt
     9541 aatcgtatta aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac
     9601 aaaacgcgtg aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata
     9661 ttttttatcg aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact
     9721 tactgaaatt taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc
     9781 tgaaaagggg ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc
     9841 gctttttgct ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca
     9901 catcatttgc gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta
     9961 aaatcaccca gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa
    10021 caggtaaacc tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca
    10081 tcgtctggaa cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga
    10141 aagttctgga taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc
    10201 gtaaaccagt ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg
    10261 gtgtagatac cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg
    10321 ttgcggggac gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag
    10381 gtgagctgat tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag
    10441 gtaaagacct gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg
    10501 atgacatggc gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc
    10561 atcgttagat ggcgcttttt atccggtgcg ccgtaaaacc c
//
LOCUS       DAJTYL010000121        10475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYL010000121 DAJTYL010000000
VERSION     DAJTYL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.121"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            21..371
                     /locus_tag="OO572_003263"
     CDS             21..371
                     /locus_tag="OO572_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6662161.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            424..819
                     /locus_tag="OO572_003264"
     CDS             424..819
                     /locus_tag="OO572_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6662162.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            860..1603
                     /gene="cmoA"
                     /locus_tag="OO572_003265"
     CDS             860..1603
                     /gene="cmoA"
                     /locus_tag="OO572_003265"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6662163.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1600..2571
                     /gene="cmoB"
                     /locus_tag="OO572_003266"
     CDS             1600..2571
                     /gene="cmoB"
                     /locus_tag="OO572_003266"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6662164.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2736..5165)
                     /gene="torZ"
                     /locus_tag="OO572_003267"
     CDS             complement(2736..5165)
                     /gene="torZ"
                     /locus_tag="OO572_003267"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6662165.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5190..6290)
                     /locus_tag="OO572_003268"
     CDS             complement(5190..6290)
                     /locus_tag="OO572_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6662166.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6678..7424)
                     /gene="cutC"
                     /locus_tag="OO572_003269"
     CDS             complement(6678..7424)
                     /gene="cutC"
                     /locus_tag="OO572_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6662167.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7438..8004)
                     /locus_tag="OO572_003270"
     CDS             complement(7438..8004)
                     /locus_tag="OO572_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6662168.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8220..9953
                     /gene="argS"
                     /locus_tag="OO572_003271"
     CDS             8220..9953
                     /gene="argS"
                     /locus_tag="OO572_003271"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6662169.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10130..>10475
                     /locus_tag="OO572_003272"
     CDS             10130..>10475
                     /locus_tag="OO572_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6662170.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSN"
BASE COUNT         2540 a         2685 c         2532 g         2718 t
ORIGIN      
        1 acgggaggtg tatttagacc ttgctaatct tagacagccg cccggttcat gcatcacgtc
       61 cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg
      121 tactgacggc gacaaatccg ctgatccgtt ttatcggtag tgatgatatg acgcaaaacc
      181 gggaattatt tcaggtctgg ttacaaaaat tagcgcagtg gcatcagacc actacgcctt
      241 atcttttttt acatacgcca gatattgccc aggccccgga actggtacat accctgtggg
      301 aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt
      361 ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt
      421 tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt
      481 gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa
      541 ctgcaaagcg ctatttgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg
      601 ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc
      661 gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg
      721 cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt
      781 tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt
      841 tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag
      901 actgggcgac tggacctttg atgaacgggt cgctgaagtc ttcccggata tgatccagcg
      961 ttccgttccc ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt
     1021 tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt
     1081 gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat
     1141 gattgaacgc tgccgtcgtc atattgacgc ctataaagcc cctacgccag tagacgtcat
     1201 tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac
     1261 cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact
     1321 gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg
     1381 tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga
     1441 gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca
     1501 taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa
     1561 ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt
     1621 tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt
     1681 gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cacggtggaa
     1741 tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc
     1801 gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg
     1861 ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc
     1921 gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg
     1981 gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc
     2041 gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta
     2101 ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg
     2161 aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag
     2221 catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg
     2281 gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt
     2341 aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt
     2401 gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa
     2461 tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt
     2521 gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt
     2581 attactgccg gatgcggcgt gaacgcctta tccggcctac aaacccttgc taattcaatc
     2641 tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat
     2701 aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca
     2761 aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta
     2821 ccggcacagg catttgcccg ctgcgagctg gggatatccg ccgttaacac gttcgcactg
     2881 ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg
     2941 ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag
     3001 acacgcacca gatcgccatt cgcaatacca aatcgagcag catcttcggt gtgaatagtg
     3061 atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     3121 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     3181 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     3241 tccagcgttt tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc
     3301 ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt
     3361 tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac gcgttgcgca
     3421 cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     3481 tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc aaacacgtca
     3541 aaatcgttac gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg
     3601 ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat
     3661 acgatatctg cgtgtttggc tgctgcggtc cagtagcatt cagaaacgac gatcatctcc
     3721 ggtttctgcc aggctttgat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     3781 cccgcccacc agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat
     3841 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg
     3901 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg
     3961 ccgccaacac gcgtcgggtt gccaccattg gagtaatgat aagagaaacc aaaaccgccg
     4021 cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt
     4081 tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg attcgccgcc
     4141 atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgtccag
     4201 accgcgctct tcggcgtatt atcgctctta cctgtcagat actcttcaaa ctgcggatac
     4261 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     4321 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta
     4381 tcgccaaaga attcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     4441 gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt
     4501 tttaaggtat tcagcgggtt cattccccac agcaccacaa cctggctgtt ttccagaatc
     4561 agcggccagg aggcctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc
     4621 acctgtgctg ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
     4681 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtaa
     4741 gatccggcaa aaatggccga tggtccgtta gctttacgaa tacgctcatg ttgctcatga
     4801 atcagtttta atgcctgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
     4861 gccggttgca gtggattgtc gagatagctt tttctcacca tcggatgctg aatacgcgcc
     4921 gtggtgtgta cctgatccgc cgccgtagac tgtaaggagt tcggtatggt tttcgccagc
     4981 gcgcctgtcg aagaaacaat cttgccgtcc ttcacttcta cgctcatcgc tccccaacgt
     5041 cccgcggtga ggattttacc gcccttctct tctgcccatg cggggagcgg tgctgccgat
     5101 gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg tcttgttaat
     5161 gccataactt cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg
     5221 tactggaaat accgcgttaa aatgtccggt tcgttttcgc tcatgctggt tcgtgccccc
     5281 attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc aatagaggca
     5341 tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag cggtttacgg
     5401 tccgctaatg cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca ttgattgccg
     5461 tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc ttcacttaat
     5521 gtggctagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt
     5581 tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac ggtgagatcg
     5641 gtaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg ataaatatgt
     5701 gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc
     5761 atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca ggtttcgccg
     5821 cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc aatatccatg
     5881 gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa ttctttccat
     5941 actgtttcgg ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc tattttgccg
     6001 ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa ataatccatc
     6061 cctgactttg gaatatggca atcggcacat tccgcacgta tccctttctg gttcgaaaag
     6121 tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat
     6181 gctgtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc gccaccaacc
     6241 acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgcat agattcctct
     6301 aaataaattc cagaacaccg aaatcattgt ataattaaat acacaacgaa ttaaccgctt
     6361 gcataactcg gcacaacact gcctgaaata atcgataaag aatatgattt attacaatgt
     6421 aatcattaat tgctaaggaa taacccagtt gctatttaga atatatattt atcagtttta
     6481 gcaatttaaa tcccataatc aatgtgaata taaaaaatat ttttagcaca acgctatgcg
     6541 cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa
     6601 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
     6661 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
     6721 accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg
     6781 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
     6841 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctcca
     6901 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
     6961 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
     7021 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
     7081 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg
     7141 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
     7201 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
     7261 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag
     7321 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
     7381 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
     7441 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
     7501 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
     7561 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     7621 acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt cccaaccttc
     7681 gtgtgggtaa cgtttttccc cgggccacgg tagctccaca ttggaaaact gccagtgcgc
     7741 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcattttttc
     7801 tgacaaaagc tcgccacact gttcaaaacc gcgacgccag cgctcagcgg tgacgttttg
     7861 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atatccaacc ccagacggcg
     7921 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     7981 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     8041 ccctgttgcc ctgtgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     8101 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     8161 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     8221 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     8281 ctgcggattg cgaaccgcag attcgtcagt cagcaaaagt tcagttcggc gactatcagg
     8341 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     8401 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     8461 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     8521 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
     8581 cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt attggtgaca
     8641 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     8701 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     8761 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     8821 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     8881 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     8941 tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc gaaagcctct
     9001 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     9061 gcgaaggggc gaccgtcgta ttccttgatg tgtttaaaaa caaggaaggc gaaccgatgg
     9121 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     9181 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     9241 agcatcaaca cctgatgcag gcatgggcga tcgtccgtaa agcaggctat gtaccggaat
     9301 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     9361 aaacccgcgc gggtggtacc gtgaaactgg cagacctgct ggatgaagcc ctggagcgtg
     9421 cacgtcgtct ggtggcagaa aagaacccgg atatgccagc tgacgagctg gaaaaactgg
     9481 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     9541 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     9601 agtatgctta cacgcgtgta ttgtccgtgt tccgtaaagc agaaattaac gaagagcaac
     9661 tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     9721 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
     9781 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     9841 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     9901 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
     9961 ttcgtgagag tgaagcctga tcagggttag ccgatcaggc ttttttattg ccatctaaat
    10021 gtattctgaa aacggacatg ccattgtttt ctcactgttg agtaagaggc cagaagcgta
    10081 atatccaggc ccaggaaaac gataacggtt caatttaagg aataccgaag tgtttaaatt
    10141 tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata cggttatagt
    10201 aaacgaccat gacacttcag ccatcaaaga ttgttggcaa aaaaattcag atgatgatac
    10261 tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg atgcgcaatt
    10321 aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
    10381 aaaacttgat actgaacaac ttaacttgct taaaaaatca cagcgagcct ggctggattt
    10441 tagggacaaa gaatgtgaat taatcctttc aaatg
//
LOCUS       DAJTYL010000122        10435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYL010000122 DAJTYL010000000
VERSION     DAJTYL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.122"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1069)
                     /gene="rspA"
                     /locus_tag="OO572_003273"
     CDS             complement(<1..1069)
                     /gene="rspA"
                     /locus_tag="OO572_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6662171.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHN"
     gene            complement(1275..1601)
                     /locus_tag="OO572_003274"
     CDS             complement(1275..1601)
                     /locus_tag="OO572_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6662172.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1736..2077
                     /locus_tag="OO572_003275"
     CDS             1736..2077
                     /locus_tag="OO572_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6662173.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2143..2672
                     /gene="speG"
                     /locus_tag="OO572_003276"
                     /pseudo
     CDS             2143..2672
                     /gene="speG"
                     /locus_tag="OO572_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2675..3385)
                     /locus_tag="OO572_003277"
     CDS             complement(2675..3385)
                     /locus_tag="OO572_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6662174.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3493..3798
                     /locus_tag="OO572_003278"
     CDS             3493..3798
                     /locus_tag="OO572_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6662175.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3997..4317
                     /locus_tag="OO572_003279"
     CDS             3997..4317
                     /locus_tag="OO572_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6662176.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            4877..5455
                     /locus_tag="OO572_003280"
                     /pseudo
     CDS             4877..5455
                     /locus_tag="OO572_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6141..7130)
                     /locus_tag="OO572_003281"
     CDS             complement(6141..7130)
                     /locus_tag="OO572_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662177.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7215..7706)
                     /locus_tag="OO572_003282"
     CDS             complement(7215..7706)
                     /locus_tag="OO572_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662178.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8027..8527)
                     /locus_tag="OO572_003283"
     CDS             complement(8027..8527)
                     /locus_tag="OO572_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662179.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8517..9032)
                     /locus_tag="OO572_003284"
     CDS             complement(8517..9032)
                     /locus_tag="OO572_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662180.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9463..9831)
                     /locus_tag="OO572_003285"
     CDS             complement(9463..9831)
                     /locus_tag="OO572_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662181.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10243..>10435)
                     /locus_tag="OO572_003286"
     CDS             complement(10243..>10435)
                     /locus_tag="OO572_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662182.1"
                     /translation="GDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDL
                     YGKGHAVGRLADVLKTALL"
BASE COUNT         2686 a         2638 c         2580 g         2531 t
ORIGIN      
        1 agttgtgcgg gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga
       61 aattggggac ccacagatca aagtgcagcg ccgcagccat gcagactggt gacaaatcgg
      121 aaggaccgtg tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca
      181 taccggtaat tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt
      241 caatcagttg tttgcagtcc cagatgctgt tgaagacttc acccactgcg atgggtgtga
      301 cggtatgttg gcgaatgaga cggaagcatt cttggttttc cgctggcgtc gggtcttcca
      361 tccagaacat gcgataatct tcaatgcttt taccaaagcg cgccgcttca ataggcgtta
      421 agcgatggtg catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt
      481 caaacaattt cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc
      541 actgtccttt ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg
      601 ttttcatacc agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac
      661 gggcataatc atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca
      721 ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg catgttggca gctttggctt
      781 taatatccca cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac
      841 gccagtaagc acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac
      901 caataagccg cggacaaaga tgatcctgca aataagaggc cacggaaagc tcacgtccat
      961 tgagggtggc atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga
     1021 aattacgccc cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct
     1081 tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag ctactgccat acaagtatga
     1141 agatcgaaaa aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg
     1201 acaaaagatg aaatagaata ccttttgtca gctgacactt cctcgtatct tattgataaa
     1261 atggatttat gttcctacgt gcgcccccag cccgcaacaa tgatcaacat tccgcaaagc
     1321 gcaatcaacg cacccgtcca gtcataaaga cttagtttca cgccatccac aacgcgcagc
     1381 cacatcaatg ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc
     1441 gcaggatgca acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac
     1501 agccagatac tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca
     1561 cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat
     1621 catgtatagt tatagggcga cataatatca tcaatataaa catcctcctg gtacgttttg
     1681 cgttcgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
     1741 aatcactctc agcaaacgaa tcggcctgct cgcttttctg ctgccttgcg cactggcatt
     1801 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
     1861 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
     1921 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
     1981 cgataaatgt gagcaaagtg ccaacatcaa tgcctactgg gagcccaata ctttgcgctg
     2041 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
     2101 aaggacacgt tatgccaagc gcccacagtt aagctacgcc cgctggagcg tgaagattta
     2161 cgctatgtac atcaactcga caataacgcc agtgtgatgc gttactggtt tgaggaaccc
     2221 tacgaagcct ttgttgaact ctctgatctg tatgataagc gcatattcac gatcagagcg
     2281 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
     2341 ttaaccatgt tcatcgccgg gcagaatttc agataattat ctccccggag tatcagggga
     2401 aaggtctggc aacccgtgcc gccaaattag caatggacta tggcttcacc gttctcaatc
     2461 tctataagct gtatctgatc gtcgataaag agaatgaaaa agcgattcac atttaccgca
     2521 agcttggctt tacggttgaa ggtgaattga tgcatgagtt ttttattaat ggtcaatatc
     2581 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
     2641 gtcagactct cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga
     2701 tggtgtaaac ccgttcgacg gcgggtttta ctccttcatc aacaatgatc agctgacagt
     2761 ccactggatt ggcgtgactg tcatattcac agctctgttt tacattacca actcgttgat
     2821 tatttagtaa agtaaccgct gtgtaatcta attttttgat cggatccgtt gatggcgtgg
     2881 cgctgacaga taatgttttg tcgttacttt tcgtggtttt acccagcgga tacccctgat
     2941 catcatagcg atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat
     3001 catcggtttc ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta
     3061 aacgtactct cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag
     3121 cgaccacggt attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg
     3181 acaaagtccc agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga
     3241 aatcttttac cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct
     3301 caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta
     3361 agcatggtag taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca
     3421 tagtagcaaa tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt
     3481 aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca
     3541 cccgcggtac tcgccgcgcc gggatcttgt gagcgcatac agagcgatat atcacagcgc
     3601 attatcaata atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag
     3661 gttgatcagc ctgattccca ggtcgtcggg cattgcgcta atgatacgca taaaattctc
     3721 tacaaccgca ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg
     3781 cctgccgaac cgcagtaaca cattatcgac tgaacgccgg atatgacaca tccggcgatt
     3841 tgaacataca acataatctc accttattac tcataccctt ctattgatgt ggattaataa
     3901 ttcttaaccc aaaatgggta gactccctct attgttagcg cgctaaatat tcaatgtata
     3961 aacatttata taacaataaa gaacagggag tgagttatgt ccaaaaatga ccgaatggtg
     4021 ggcatcagcc gcagaacact cgttaaatca accgcgacag gttctctggc gctggctgca
     4081 ggcggttttt ctttaccgtt caccctgcgc agtgcagcgg cagcggtaca acaggccagc
     4141 gaaaaagtga tctggggttc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta
     4201 catgttaaag ataatgaagt gacctgggtg gaaactgaca ataccggcag cgatgagtac
     4261 ggcaaccatc agggtacgcg cctgtttgcg cggtcgctcc atccgccggc gtattaatca
     4321 tcccgatcgc ttgaatgacc caatgaaacg cgtgggtaaa cgcggtgaag gcaaattcga
     4381 acggattagc tgggatgaag ccctggatac tatcgccagt agcctgaaaa aaaccgtcga
     4441 acaatatggc aatgaagctg tatatattca gtactcttcg gggattgttg gcggcaatat
     4501 gacccgctct tcgccatcag cctcggcggt caaacgcctg atgaactgct acggcggttc
     4561 actcaatcag tatggctcct acagcaccgc gcaaatttcc tgtgccatgc cctacaccta
     4621 cggcagtaat gatggcaaca gcaccacgga tattgaaaac agcaagctgg tcgtgatgtt
     4681 tggtaacaac ccggcagaaa cccgcatgag cggtggtggc atcacttatc ttcttgaaaa
     4741 agcgcgcgag aaatcgaacg ccaaaatgat tgttatcgat ccgcgatata ccgatacggc
     4801 tgccggtcgt gaagaggaat ggctccctat tcgcccgggc accgatgccg cgctggttgc
     4861 gggtattgcc tgggtattga ttaacgaaaa tctcgttgat caaccttttc tcgataaata
     4921 ctgcgtcggc tatgacgaaa aaaccttacc cgcagatgca cccaaaaatg gtcactataa
     4981 agcctatatt cttggtgaag gtgacgataa aacagcaaaa acgccgcagt gggcttcgca
     5041 aattaccggt atcccggtag accgtatcat caaactggcg cgtgaaattg gcacaacaaa
     5101 acccgcttat atctgctagg gctgggggcc gcaacgccag gcaaacggcg agctgactgc
     5161 acgcgctatt gctatgttac ctattttgac gggcaatgtc ggcatcagcg gcggaaatag
     5221 tggcgcgcgt gaatcgacct ataccattac gatagaacgc ctaccggtgc tggataatcc
     5281 ggtcaaaacg tcaatctcct gcttcagctg gacggatgct atcgatcatg gcccgcaaat
     5341 gacggcactc cgcgacggtg tccggggcaa agataaactg gatgtgccca ttaagttcat
     5401 ctggaactac aaggacttga agcggaccct tagtaggcct gaggtcctgc attgagtcaa
     5461 gaccttgtgt cattctttgt gccagacttg tgtcattaga cgggatgtct agacggctgg
     5521 tcaggaccct ccggtggcct cggtctggac ttgaggcggg ctatggggga agtctgctgg
     5581 cttaatgtat tagatggtca ttcacatttt tctgtcagaa accatcggtc ccactgtgag
     5641 tacaaaccta agcccccaga agcccaccca cagccttaga cagagtgtcg aatacattca
     5701 caccgagtcg accggagagg accatataga ggcccacaga gaggaacacg agtttataga
     5761 ccagagtcag gagggacctt aggtcttcgg aagtcatgga cataaagccc ccccctatct
     5821 tctattgcac taccgcacta gacaactagt acaaaacaaa ggcccagacc attggtccag
     5881 accgtagtta gttaatcaca gtgacaagcc accacctgaa gtcctgctga tattcagtcc
     5941 agacttacct ctgctcatgg tcttacctcc ctcggtctcc tgaccttggg tatcgactac
     6001 agcttgctga gcgtcatctg gaggagctac aggaggagct acaggaggcg ggaccgaagc
     6061 cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga agcctttact
     6121 aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg tttgactaca
     6181 ggcaggacgg cagtagctga gccagtgccc atcttgatgg tcgtagcagc ttcagcgaca
     6241 acgaacactt taccgaggat ttccaagtcg tgggactgag agtcgccatc acgtgaaagg
     6301 acgaccgtaa tattctcacc gtcgtgacgg aagcagccga agctcagggc cgtgaagcgt
     6361 ttagccagca tgaatacacc cttgtcttca ccgttgtaat aggcgatgtc tgtccccagc
     6421 atatcgacga tagcgtcttc accggagaag ccttgcttag cagccagacg ggacagtgct
     6481 ttcagttggc tctcatgagc gataataccg tagtcagaca cctcagaacc gaaagaagac
     6541 tctgtgttct catacagagc gtccatgata gcgtctgcct tagcggtcag gctaccagtc
     6601 aggacaggaa gctctacggt cttcagcagc ttagaggatt ccagaacgtc gaccatgcga
     6661 gcgttgatgg tacggaccac attctcacag atgttcatag cgtcctgctg aagtgtgccc
     6721 tgagtgaatc cggtggatga cttggcgtcc cagagcttgg tctgctcaga gatgagaata
     6781 gcctgctcga ttggttcgac cacgatttct tgtccagacg gaacggtcac gacttcaggt
     6841 cgcttgccac cacggacagg aacgaccaca gagcggtcag ccagagtgtg agcaatgatt
     6901 ggggtcggta agctgttacg ggtcggcagg acttctacag ggaacgggat gttctcttga
     6961 gcgtctttaa gtacaccgtt cagcatagcc ttaaccgtct tagctaactg gagggcatag
     7021 acctgcgggt cagcgttagt cggcagacct tcattgacct gaatgttgtc ggtatcggtg
     7081 atgcgtactg gctcaatgcc aaactggaaa gcgtctttaa tcgtagtcat aataatcaat
     7141 ctcctaaatt agtttaaata ctgtgagtcc tcaaaactga ggagtcccta acatcgggtc
     7201 agggactgtg tgttttacaa gctgtccatc cagttaccgc ctgactcaag gtcagcgaca
     7261 atagcggcac gggagcgacc agcggaatgg gtagcactgc ggaggctctc accataggtc
     7321 cggcgttcag ctttgacctg ttcgcggcgc tgtagctctt tctgccactc tgcatcggtc
     7381 atgttctctt cacgcatacg ctcagcctga agtcgttgct ctttagcgat acgctcagct
     7441 tcagctcgtt tgttcatctg gcggcgacca taggcggtca tcaagtcatt actgaagaag
     7501 ttgccaatgg tcacgccagc ttcagccaga gcttccagta actccattgc ctcgcggctg
     7561 gtagacggca ggcctgacag gtagcccgga atgagggtct tgatagcgag cgacagcgga
     7621 tagggctgac cgtccagact cacagtgttt tcataaaggt tgatgtcggt gactgacagc
     7681 ttccagttgt tgttgttgaa catcataggt aattctcctc ttgagagttg agactatcgg
     7741 tatcgcagac gcagtagcgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa
     7801 attaggtttg gtatggtcag gactgcttag caaaggctcg gcgtaactga ttgtctaaat
     7861 cacggtccac cagcatagct atcagctcgt ctcgactgta gtaagggcgc tcagggtctt
     7921 cgactccaag tctcagcaga gacagcagat gacgtgtggg caggccacgg taacgaaggg
     7981 tcgctttgcc gataggtgta cgagggtcaa tcatagccat gattacttac ctccactcac
     8041 agcgaccagc ggtcacagct tgatagtctg aatactcagc ctgtgagact tcacgtccgg
     8101 cgatgtagag gcctgtgcca tgacgctgga tgaattcctt ggtctccggc ttgatgtcct
     8161 cagcagacgc tacagcagac tgcacggtgg cgctggaggt catagacacg aaggctcggg
     8221 ttactgccag tccgtaccca aggtccaacc accgaccgaa ctctatagcg gctactgggt
     8281 cagccagcca cgaggtccct aagtgaccca tgttaatctt tttaacaaac ccgttttctg
     8341 ggttggttag acttctacca gccttggtcc tcatgaagcg agcaacctcg gtactacggt
     8401 tatcttctac cttaccaatc tgaatgccta cccggcgtat ctcatccagt cgaaacaatc
     8461 cgtcctgact ggtgatgtcc tgaccgaaga tatgaatggt gtattcctgc ttattgtcag
     8521 aagtcataag aggcacctcc cgccagagct tcggcaaagg acttgaagcg ctgttcttgg
     8581 tcagctttcg cctgaaggcg ttcggcatca ggtgggacca gcttatcaaa ggtgccgttt
     8641 tcgtaatctt cagttaggac ctgaaggcgg cgggaccatt tgctctccac ctgcatcatc
     8701 gcgttagaga tgtgcttcaa gtcggcatca gagcgtgacg ctatccaatc catctcttct
     8761 gagtcaccct cacaggtgta aggattgttg aaatagaaga tgtcctgcag gccacgcttc
     8821 agctcttcct cggtgtgtgt ctggaggtat gactggatag gctccgcgta catctcccgg
     8881 attttctcat agtcccagtc ttccgcctgg gcataccatc gggataactt cagcaggtct
     8941 aagttatagc gacagcaatc gacactggac tctgcattgg tcagcaggat gattaagaac
     9001 tggcggttag ttactggtaa ttcggtattc atacaaagat gctcctttgg taaagcactt
     9061 tctgtctcca catggagtcc gcaccattca gacgcatcct aaacggaccc atcagaaagt
     9121 gcttaacgaa agtgaatggt taaatgttga cggtctggag tgaccatgtt ctccatgtac
     9181 gtataaaggt gcgaccgcat gtcaggacga gctgtaagga ctccagatgg tgagcggtga
     9241 tagacttaag ccaatgtagt gagtagacga gctactaacc gagacgacta aagaacgcta
     9301 acgagctgga ggtcgataca ggaggtctgt cagaggcggt cacgggcctt gattacttag
     9361 gagagatact taatgtcggt tgaccagatg ctcgcatgtt ctgacgcatc agactcagag
     9421 gcggaggctg gtctccctat agtgatagtt tagggtccgt aatcatgaac cagctttact
     9481 ggcgtggtac tggacggatt tactggcgct gacgacggta gcatagacgt agcgagcgta
     9541 gcgagttgaa accataggtt cacccaaggc aacacgctta cggctcaggg actcctgcac
     9601 agcgcttaca gttaatgtgt cgtgcgttag tagttgggtc agaatcaagt atgggctgac
     9661 aggcttggta ttgcttgcca ttgattcgtt caggcgacca gcaccagtaa ggacatcatc
     9721 cagccaacca ctcaggtcgc cctgaccatc gaatgacagc aggtccccgc agtgctgctg
     9781 caagtaggct ttggtgaacg tgttgtcgtg agttgcgatt ggtcgtgcca tagagtatct
     9841 cctgtagttg aattagttgg ttcccctata gtgataatca agtccatcac tggtgatgca
     9901 tacagtagcg cgacaggatg tgactcacag gttgaaccga atgtcaagga ctaaagacgc
     9961 ctcaggaatt aaggaagtat atataaagag agaccagagg cgactcacag ttaaacagga
    10021 atgtcaagag gagaccatag gaatttggag ggactggagt ttaggacgca gtttaaataa
    10081 gaccacagac agacttagag tcggacagcc gaaggcggac cagtgggcct atagtcagga
    10141 gggattatga ttattatcac cctccctaac ttcagtcaga cttcagtgac ccaaggtctc
    10201 aaccatcaag ccgtatatgg tctccacctt gggtctccct acttaaagta aagctgtctt
    10261 aagcacatca gccagcctac ccacagcgtg tcctttcccg tacaggtcat acgtaattga
    10321 ctgactgctg tgacctaaga tgccttgagc gtccaccaga ttgactccac gctctttcat
    10381 tcgggtcgcc agccaatgtc tcagactgtg gagagagaca tcctgagagt ctcct
//
LOCUS       DAJTYL010000123        10268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYL010000123 DAJTYL010000000
VERSION     DAJTYL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.123"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            311..508
                     /locus_tag="OO572_003287"
     CDS             311..508
                     /locus_tag="OO572_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662183.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(458..1651)
                     /locus_tag="OO572_003288"
     CDS             complement(458..1651)
                     /locus_tag="OO572_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6662184.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1787..3511
                     /gene="iucA"
                     /locus_tag="OO572_003289"
     CDS             1787..3511
                     /gene="iucA"
                     /locus_tag="OO572_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6662185.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3512..4459
                     /gene="iucB"
                     /locus_tag="OO572_003290"
     CDS             3512..4459
                     /gene="iucB"
                     /locus_tag="OO572_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6662186.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4459..6201
                     /gene="iucC"
                     /locus_tag="OO572_003291"
     CDS             4459..6201
                     /gene="iucC"
                     /locus_tag="OO572_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6662187.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6198..7535
                     /gene="iucD"
                     /locus_tag="OO572_003292"
     CDS             6198..7535
                     /gene="iucD"
                     /locus_tag="OO572_003292"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6662188.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7541..9736
                     /gene="iutA"
                     /locus_tag="OO572_003293"
     CDS             7541..9736
                     /gene="iutA"
                     /locus_tag="OO572_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6662189.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2485 a         2631 c         2726 g         2426 t
ORIGIN      
        1 tgaattaaaa accagtaaaa atacccgaca agaaatctgc aatgatttac ttcataaaaa
       61 acatgtcagt gaaaaagaaa cctggcgttg ggttgaagga tataataaag caacagaact
      121 ggctcgccaa tgccctgata cccatgtatt gtcaatttct gatcgtgaag gcgattttta
      181 tgacctgttt gaacgcgcag cgcagactcc cggaataaaa gctgactggc ttgtcagaat
      241 gaaatttaat aacagagcaa cgctgaatat taatggtaaa cgggatcata ggttgttgca
      301 cgaaagaatc atggagatta caccgcagca actggttgaa ttcactatac ctgatgggcg
      361 ggggcagcaa gccagaacac tgcctgacat gctttcatca gacgcaggtc ctctctcata
      421 tcctctgatt tactccagta aggtgatgat catatcttta tttgatagta cggtaatgcc
      481 agataaatac agacacaatg gtaaataata tagttgcaaa taatgctgcc ataatcagcg
      541 cgattgcgac acctgttttc gataccagcg gcaccagcaa agcgcacagt ccatagccaa
      601 gtgtgtgact ggtagcgagc cagcccgcgc ctgcgccatc agccagctta tcattcagta
      661 gaagttgata agcgggtgtt gccagagctg ctccaaatga cagcactgca cagcccgggt
      721 aaaacaacca tagctgttcg gagagcatga tagccagacc accactcatc aacactccgg
      781 cactcaggag cagggccacc ggagtcaggc gctgcggacg gagtaccccg aactgcgcga
      841 taagcgcagc tactgcggac agacccaaca gccaggccac ctgttggcta atggcggtgg
      901 tatccgtagc gaactggcga gtaagggcag gcgaaagtcc aagctgcatc atgctcaccg
      961 ccgcagccag taatattgcg caaagcagat aaggcagaca atcccgcttc agactgacgc
     1021 tcttgcactc cggtgtgggc tgtggtggtg tgccgggcaa ccgcagcagc atcagcagcg
     1081 ccagcactgg ggctgccatc agcagcccca gtggcgccag aggatgaatg gccagcattg
     1141 ctgccgcgca cagcggcccg aatagcctgc cgcaactcag gccggagctg atggttgcca
     1201 gagcggccat gcgattccct tctccagctc tctgcaatgc ccagacctga caggctggca
     1261 ccattgccga cacggtcagc ccgtaggcga tccgggcgat gattaatatt cccagtccca
     1321 ccgctgttgt tatggcgctg gtagccatca gcacgcttcc cagccctagc agggtaaagc
     1381 ttatgccata tcctcccaga gcccacaaca ccacaggttt actgccagta cgggaaattt
     1441 gctttcccca ccacggggac gatggcagaa acagcattga gccaagcatc agcagggcag
     1501 cccatacaga cagactcaga tttgtctgta tgaccagaac agggagcaca accagcagac
     1561 cgttctgtcc gataccgaga agcccggcac tgaacgcaag tggccagcag gacagtggtt
     1621 tttggggcgt atcttcgatc cctggtgaca ttataatgtt tcaactccat gtattaattg
     1681 tgtttatttg taaaattaat ttatctgaca ataacattta ttgttgataa tgagaatcat
     1741 tattgacata attgttatta ttttactgtg tgggagctgt ttgactatga ccctgccctc
     1801 tgaaaaacca gccacagatg tggctgcgca gtgcttcctg aatgcactga ttcgtgaaac
     1861 cacagactgg aaactgacag aatacccgcc agacgaattg ctcatcccgc tggatgagca
     1921 gaaatcgctc catttcagag tggcttattt ctccccaacc cagcatcacc gctttgcatt
     1981 tcctgcacgt ctggtcacgg catcaggcag ttatcctgtc gactttacca ctctctcccg
     2041 gcttattatt gataagctac gccatcaact gtttctgccc gttcccctct gcgaaacttt
     2101 ccaccagcgc gtgctggaaa gccatgtcca tacgcaacag gcaattgatg cccgtcatga
     2161 ctgggccgct ctgcgtgaaa aagcgttgaa ttttggcgag gctgagcagg cgctgctgac
     2221 aggacacgct ttccaccctg cgcctaagtc tcatgaaccg tttaaccggc gggaggctga
     2281 acgctacctg cctgatatgg caccccactt cccactgcgg tggttttcgg tggataaaac
     2341 gcaaatcgcc ggtgaaagtt tgcatctcaa ccttcaacag cggctgacgc gatttgccgc
     2401 agagaatgcg cctcagttac tcaacgaatt aagtgacaac caatggctgt tcccgttgca
     2461 cccgtggcag ggggaatatc ttttgcagca ggggtggtgc caggcacttg ttgctaaagg
     2521 gctgattaaa gacttaggtg aggccggcac gtcgtggctg ccgaccacct cttcccgttc
     2581 cctctactgt gccaccagcc gcgatatgat caagttctcc ctgagcgtac ggctgaccaa
     2641 ctccatccgt actctgtccg tgaaagaagt gaagcgtggg atgcgcctgg cacgcctggc
     2701 tcaaaccgac ggctggcaga tgctacaggt ccgcttcccg actttccggg taatgcagga
     2761 ggatggctgg gccgggctgc tcgatcttaa cggcaacatc atgcaggaaa gtctgtttgc
     2821 cctgcgtgaa aatctgctgg tggatcagcc gaaaagccag actaacgtac tggtctccct
     2881 gactcaggcc gcacctgatg gcggtgattc gctgctggtt tcggcggtaa aacgcctgag
     2941 cgatcgcctc ggtatcactg tgcaacaggc cgcccatgca tgggttgatg catactgtca
     3001 gcaagtgcta aagccgctgt ttacggctga agcggattac ggcctggtgc tgctggcgca
     3061 tcagcaaaat attcttgtcc agatgcttgg ggatctgccg gtcggattta tttaccgtga
     3121 ctgtcagggc agcgctttta tgcctcacgc gacagactgg ctcgattcta ttggcgaggc
     3181 gcaggcggaa aatattttca cccatgagca gttgctgcgc tatttccctt attacctgct
     3241 ggttaactcc acttttgctg tgaccgctgc gctgggggct gccgggctgg acagcgaatc
     3301 gaatctgatg gctcgtgtac gagcatcgct ggctgaagtg cgtgatcagg tgactcataa
     3361 aacctgtctc aactacgtgc tggaaagtcc gtactggaac gtaaaaggta actttttctg
     3421 ttatctgaac gatcataacg agaacaccat tgttgaccct tcggtgatct atttcgattt
     3481 cgctaacccg ctgcaggctc aggaggtctg aatgtctgag gcaaacatta ttcacagcag
     3541 atatggactg cgctgtgaaa aactcgacaa gcccctgaat cttggctggg gactggacaa
     3601 tagcgcggtg ttgcactgtc ccggggagct gccgacaggg tggctgtgcg atgcgctgga
     3661 tcagatattt atcgccgcac cacaactgtc agcagtggct ctcccctggg ccgaatggcg
     3721 tgaggagcca caggcgctga cgctttttgg gcaggtaaaa agcgacatta tccatcgtac
     3781 tgccttctgg cagttaccgt tatggttgag ttcaccggca aaccgggcct ccggtgaaat
     3841 ggtttttgat gcagagcgtg agatttattt cccgcagcgc ccaccccgtc cacagggtga
     3901 ggtttaccgc cgttacgatc cacgtattcg caggatgctg agtttccgca ttgccgatcc
     3961 cgtttctgat gcagaacgtt tcacccgctg gatgaatgat ccgcgcgttg agtatttctg
     4021 ggagcaaagt ggctcactgg aggtacagac cgcctatctg gagcgccagc tcaccggtaa
     4081 acatgcattc ccgctgattg ggtgcttcga cgatcggcca ttcagctatt tcgaaatcta
     4141 ctgggcggcg gaagaccgca ttggtcgtca ctattcgtgg cagccctttg accgtggcct
     4201 gcatctgctg gttggtgaac agcaatggcg cggggcccac tatgtgcaaa gctggctgcg
     4261 cgggctgaca cattacctgc tgctggatga gccccgtacg cagcgcacag tactggagcc
     4321 acgcaccgat aaccagcgcc tgttccgcca tcttgagcct gcgggatacc ggacaattaa
     4381 agagttcgac ttcccacata agcgctcgcg catggtgatg gcggatcgtc atcacttctt
     4441 cacggaggtc ggtctgtaat gaatcacaag gactgggatt ttgttaaccg tcggctggtg
     4501 gcaaaaatgt tgtctgagat ggagtatgag caggttttcc acgcggaatc tcagggcgat
     4561 gaccactact gcattaacct gccgggagca caatggcgct tcatcgctga acgtggtatc
     4621 tggggctggc tgtggattga tgcccaaacg ttgcgctgca cggacgaacc agtactggcg
     4681 cagacgctgc tgatgcagct aaagccggtg ctgtcaatga gtgatgccac tgttgcagag
     4741 catatgcagg atttgtatgc cacgctactg ggcgacctgc aactgctgaa ggcccgtcgc
     4801 ggactgagtg ccagtgacct gattaatctt gatgccgatc gtctgcaatg cctgctgagc
     4861 ggtcatccta aattcgtttt taataaaggt cgccgtggct ggggcaaaga ggcgctggaa
     4921 cgatatgcgc cagagtatac caacaccttc agactgcact ggctggcggt aaaacgtgaa
     4981 catatgatct ggcgctgcga taacgatctg gatattcagc agttgttgac tgccgcaatg
     5041 gatccgcagg agtttacacg cttcagtcag gtctggcagg aaaacgggct ggatcacaac
     5101 tggctgccgc tgccggtaca tccgtggcag tggcagcaaa aaatcgccac agacttcatc
     5161 gctgattttg ccgaaggcag gatggtgtct ctcggcgagt ttggcgacca gtggctcgcc
     5221 cagcagtcgc tgcgtaccct gaccaacgcc agccgccggg gagggctgga tatcaagctg
     5281 ccgctgacca tctacaatac ctcatgctac cgggggatcc ctggcagata tatcgctgcc
     5341 ggaccactgg cttcacgctg gctgcaacag gtttttgcga ccgacgccac cctggtgcaa
     5401 agcggcgcag tgatccttgg tgaaccggct gcaggctatg tgtcccatga aggctatgcc
     5461 gcgcttgccc gggctcccta tcgctaccag gaaatgcttg gtgttatctg gcgggagaat
     5521 ccgtgccgct ggctgaaacc ggatgaaagt cctgttctga tggcaacact gatggagtgc
     5581 gacgaaaaca atcagccgct ggcaggcgca tatatcgacc gctccggact ggacgctgaa
     5641 acctggctta cgcaattgtt ccgggtggtt gtggttccgc tgtatcacct gctttgccgc
     5701 tacggtgtcg cgcttattgc acacggacaa aatataacgc tcgccatgaa agagggggta
     5761 ccacagcgtg ttctgctgaa agacttccag ggcgatatgc ggctggtgaa agaagcgttt
     5821 cccgaaatgg actctttgcc tcaggaggtg cgtgatgtca catcccgtct gagtgcggac
     5881 tacttaatcc atgatttgca gacgggtcac ttcgtgacag tactgcgttt tatttcgcca
     5941 ctgatggttc gtcttggcgt acctgaaagg cgattttatc aactgctggc agcagtgttg
     6001 agtgattaca tgaacaaaca tccacaaatg gcagagcgtt ttgcgctttt ctcactcttc
     6061 aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac tgacctggcc ggacctggat
     6121 ggcggcagtc gcatgctgcc gaattacctt gagaatctgc aaaatccgct gtggctggta
     6181 actcaggaat atgaatcatg aaaaaaagtg tcgattttat tggtgtcggg acagggccat
     6241 ttaatctcag cattgctgca ttgtcacatc agatcgaaga actgaactgt ctcttctttg
     6301 acgaacatcc tcatttttcc tggcatccgg gtatgctggt accggattgt catatgcaga
     6361 ccgtctttct gaaagatctg gtcagtgcag ttgcacctac aaatccctac agctttgtta
     6421 actatctggt gaagcacaaa aagttctatc gcttccttac aagcaggcta cgtacagtat
     6481 cccgtgaaga gttttctgac tatctccgct gggctgctga agatatgaat aacctgtatt
     6541 tcagtcatac cgttgaaaat attgatttcg acaaaaaaag tcgattgttt ctggttcaga
     6601 ccagtcgggg agaatatttt gcccacaata tctgccttgg tacaggaaaa caaccttatt
     6661 taccaccctg tgtgaagcat gtgacacaat cctgtttcca tgccagtgaa atgaatcttc
     6721 gtcggccgga cctgagtgga aaacggataa ccgtggttgg tggaggacag agtggtgcag
     6781 acctgttcct taatgcatta cgcggggaat ggggagaagc ggcggaaata aactgggtct
     6841 cacggcgtaa taattttaac gcactggatg aggctgcttt tgctgatgag tattttacac
     6901 ctgaatatat ttcaggcttc tccggactga aggaagatat tcgccatcag ttactggatg
     6961 agcagaaaat gacatcggat ggcatcactg ccgattcttt actgaccatt tatcgtgagt
     7021 tgtaccaccg ttttgaagtt ctgagaaaac caagaaatat ccgtctgcta cccagtcgct
     7081 cggtaacaac tctggaaagt agtggtccgg gctggaggtt attgatggaa catcatctgg
     7141 atcagggcag agagagcctg gaaagtgatg tggtgatttt cgccacaggt taccgttctg
     7201 cattgccaca aatacttccc tcactgatgc ccttgatcac catgcacgat aagaacacct
     7261 ttaaagtgcg tgatgacttc actctggaat ggagtggccc gaaagaaaat aacatctttg
     7321 cggtcaacgc cagcatgcaa actcatggca tcgccgaacc ccagctcagc ctgatggcct
     7381 ggcgatctgc acgtattctt aatcgcgtac tggggcgtga tttattcgat ctcagtatgc
     7441 cgcccgcact gattcagtgg cgcagcggca gccggaaaaa accacagccg gaggctgctt
     7501 ctttaactca ctatacagca aatattcagg aataacgatg atgcgcaaaa agtatatgcc
     7561 ccgggctctt ggtccgctgc ttcttgtcgt gctgtcacca gctgtcgccc agcaaaacga
     7621 tgataatgag atcatagtgt ctgccagccg cagcaatcga actgtagcgg agatggcgca
     7681 aaccacctgg gttatcgaaa atgccgaact ggagcagcag attcagggcg gtaaagagct
     7741 gaaagacgca ctggctcagt taatccccgg ccttgatgtc agcagccaga gtcgaaccaa
     7801 ctacggtatg aacatgcgtg gccgcccgct ggttgtcctg attgacggtg tgcgcctcaa
     7861 ctcttcacgt tccgacagcc gacaactgga ctctgtcgat ccttttaata tcgaccatat
     7921 tgaagtgatc tccggcgcga cggccctgta cggtggcggg agtaccggag ggttgatcaa
     7981 catcgtgacc aaaaaaggtc agccggaaac catgatggag tttgaggctg gcacaaaaag
     8041 tggctttaac agcagtaaag atcacgatga gcgcattgcc ggtgctgtct ccggcggaaa
     8101 tgaccatatc tccggacgtc tttccgtggc atatcagaaa tttggcggct ggtttgacgg
     8161 taacggcgat gccaccctgc ttgataacac ccagaccggc ctgcagcact ccaatcggct
     8221 ggacatcatg ggaaccggta cgctgaacat cgatgaatcc cggcagcttc aactgataac
     8281 gcagtactat aaaagtcagg gagacgacaa ttacgggctt aatctcggga aaggcttttc
     8341 cgccatcagc gggagcagca caccatacgt cagtaagggg ctgaattctg accgcattcc
     8401 cggcactgag cggcatttga tcagcctgca gtactctgac agtgatttcc tgggacagga
     8461 actggtcggt caggtttact accgcgatga gtcgttgcgg tactacccgt tcccgacggt
     8521 aaatgcgaat aaacaggcga cggctttctc ctcgtcacag caggataccg accagtacgg
     8581 catgaaactg actctgaaca gccaactgat ggacggctgg caaatcacct gggggctgga
     8641 tgctgagcat gagcgcttta cctccaacca gatgttcttc gatctggctc aggcaagtgc
     8701 ttccggaggg ctgaacaacc ataagattta caccaccggg cgctatccgt catatgacat
     8761 caccaatctg gcggccttcc tgcaatccag ctatgacatt aatgatattt ttaccgttag
     8821 cggtggcgta cgctatcagt atactgagaa cagggtagat gatttcatcg actacacgca
     8881 gcaacagaag attgctaccg ggaaggcgat atctgccgac gccattcctg gtggttcggt
     8941 agattacgat aactttctgt tcaatgctgg tctgctgatg cacatcaccg aacgtcagca
     9001 ggcatggttc aatttttccc agggggtggc attgccggat ccggggaaat attatggtcg
     9061 cggcatctat ggtgcagcag tgaacggcca tcttcccctg acaaagagcg tgaacgtcag
     9121 cgacagtaag ctggaaggcg tgaaagtcga ttcttatgaa ctgggctggc gctttatcgg
     9181 tgacaacctg cggactcaaa tcgcggcata ttactcgctt tccaataaga gcgtggaaag
     9241 gaataaagat ctgaccatca gtgtgaagga cgacaggcgc cgtatttacg gcgtggaagg
     9301 tgcggtggac tacctgatcc cggatactga ctggagtacc ggtgtgaact tcaatgtgct
     9361 gaaaaccgag tcgaaagtga acggtcaatg gcaaaaatat gacgtgaagg aatcaagtcc
     9421 atcgaaagcg acagcttaca ttaactgggc gccggaaccg tggagtctgc gtgtacagag
     9481 caccacttct ttcgacgtaa gcgatgcaga gggtaacgat attaatggtt acactaccgt
     9541 cgattttatc agtagttggc agcttccggt gggaacactc agcttcagcg ttgagaacct
     9601 cttcgaccgt gactatacca ctgtctgggg acagcgtgca cctctgtact acagcccggg
     9661 ttacggccct gcttcactgt acgactacaa aggccggggc cgaacctttg gtctgaacta
     9721 ctcagtgctg ttctgacggg gatgcttttg caacaagggt atgctgtaaa caacatggtc
     9781 cttggtggcc atgttgttta cagccagtat aatatacagc cttaacaaga tattccgtct
     9841 ccggaatggg ctgttcacca ctgaataaca ttatgtctgt tttatggatc tttgactgaa
     9901 atttatcgca tatcctcatt tcattgtccg tctgaaaact aaccttacaa gtaagcgtcg
     9961 tctcagcacc gtctggcaga tcctgaaatt cctgagagga tgatctgcgc ccaatatcct
    10021 gagccggaac tttgctgcaa tgaaggccaa cgaaaaatgg gggagtatgt tatagagtac
    10081 ttgctacagg gtaaaaagag gtacctgtcg ctggttctcc gttcagttta accgggagat
    10141 tttctcctgc gcctgtcata aagtccggta atggagattg ttaatacgat ggtgcacgat
    10201 gcatcgtcag tgcccggccc ggaggatgcc ccgtagctac atacggatca gggttggcaa
    10261 tatcgaat
//
LOCUS       DAJTYL010000124         9993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYL010000124 DAJTYL010000000
VERSION     DAJTYL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.124"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..102
                     /gene="rseD"
                     /locus_tag="OO572_003294"
     CDS             <1..102
                     /gene="rseD"
                     /locus_tag="OO572_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6662190.1"
                     /translation="KRDTLTLCLLKLQLMEWRFDSAWKFGLGRLYLG"
     gene            99..674
                     /gene="rpoE"
                     /locus_tag="OO572_003295"
     CDS             99..674
                     /gene="rpoE"
                     /locus_tag="OO572_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6662191.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            707..1357
                     /gene="rseA"
                     /locus_tag="OO572_003296"
     CDS             707..1357
                     /gene="rseA"
                     /locus_tag="OO572_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6662192.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            1357..2313
                     /gene="rseB"
                     /locus_tag="OO572_003297"
     CDS             1357..2313
                     /gene="rseB"
                     /locus_tag="OO572_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6662193.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            2310..2789
                     /gene="rseC"
                     /locus_tag="OO572_003298"
     CDS             2310..2789
                     /gene="rseC"
                     /locus_tag="OO572_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6662194.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            2987..4786
                     /gene="lepA"
                     /locus_tag="OO572_003299"
     CDS             2987..4786
                     /gene="lepA"
                     /locus_tag="OO572_003299"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6662195.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            4802..5776
                     /gene="lepB"
                     /locus_tag="OO572_003300"
     CDS             4802..5776
                     /gene="lepB"
                     /locus_tag="OO572_003300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6662196.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            6048..6728
                     /gene="rnc"
                     /locus_tag="OO572_003301"
     CDS             6048..6728
                     /gene="rnc"
                     /locus_tag="OO572_003301"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6662197.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            6725..7630
                     /gene="era"
                     /locus_tag="OO572_003302"
     CDS             6725..7630
                     /gene="era"
                     /locus_tag="OO572_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6662198.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            7642..8370
                     /gene="recO"
                     /locus_tag="OO572_003303"
     CDS             7642..8370
                     /gene="recO"
                     /locus_tag="OO572_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6662199.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            8382..9113
                     /gene="pdxJ"
                     /locus_tag="OO572_003304"
     CDS             8382..9113
                     /gene="pdxJ"
                     /locus_tag="OO572_003304"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6662200.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            9113..9493
                     /locus_tag="OO572_003305"
     CDS             9113..9493
                     /locus_tag="OO572_003305"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6662201.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            9913..9993
                     /gene="shoB"
                     /locus_tag="OO572_003306"
     CDS             9913..9993
                     /gene="shoB"
                     /locus_tag="OO572_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6662202.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2493 a         2450 c         2695 g         2355 t
ORIGIN      
        1 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
       61 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
      121 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
      181 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
      241 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
      301 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
      361 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
      421 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
      481 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
      541 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
      601 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
      661 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
      721 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
      781 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
      841 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
      901 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
      961 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
     1021 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
     1081 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
     1141 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
     1201 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
     1261 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
     1321 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
     1381 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
     1441 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
     1501 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
     1561 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
     1621 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
     1681 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
     1741 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
     1801 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
     1861 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
     1921 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
     1981 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
     2041 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
     2101 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
     2161 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
     2221 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
     2281 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
     2341 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
     2401 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
     2461 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
     2521 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
     2581 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
     2641 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
     2701 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
     2761 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
     2821 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
     2881 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
     2941 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
     3001 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
     3061 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
     3121 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
     3181 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
     3241 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
     3301 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
     3361 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
     3421 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
     3481 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
     3541 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
     3601 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
     3661 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
     3721 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
     3781 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
     3841 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
     3901 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
     3961 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
     4021 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
     4081 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
     4141 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
     4201 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
     4261 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
     4321 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
     4381 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
     4441 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
     4501 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
     4561 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
     4621 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
     4681 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
     4741 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
     4801 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
     4861 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
     4921 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
     4981 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
     5041 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
     5101 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
     5161 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
     5221 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
     5281 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
     5341 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
     5401 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
     5461 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
     5521 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
     5581 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
     5641 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
     5701 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
     5761 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
     5821 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
     5881 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
     5941 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
     6001 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
     6061 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
     6121 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
     6181 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
     6241 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
     6301 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
     6361 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
     6421 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
     6481 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
     6541 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
     6601 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
     6661 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
     6721 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
     6781 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
     6841 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
     6901 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
     6961 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
     7021 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
     7081 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
     7141 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
     7201 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
     7261 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
     7321 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
     7381 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
     7441 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
     7501 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
     7561 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
     7621 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
     7681 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
     7741 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
     7801 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
     7861 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
     7921 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
     7981 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
     8041 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
     8101 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
     8161 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
     8221 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
     8281 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
     8341 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
     8401 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
     8461 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
     8521 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
     8581 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
     8641 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
     8701 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
     8761 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
     8821 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
     8881 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
     8941 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
     9001 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
     9061 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
     9121 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
     9181 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
     9241 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
     9301 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
     9361 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
     9421 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
     9481 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
     9541 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
     9601 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
     9661 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
     9721 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
     9781 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
     9841 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
     9901 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
     9961 gctgttttac agctgatcct tctgttctta taa
//
LOCUS       DAJTYL010000125         9904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYL010000125 DAJTYL010000000
VERSION     DAJTYL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.125"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..626)
                     /locus_tag="OO572_003307"
                     /pseudo
     CDS             complement(1..626)
                     /locus_tag="OO572_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            920..1675
                     /gene="mlaA"
                     /locus_tag="OO572_003308"
     CDS             920..1675
                     /gene="mlaA"
                     /locus_tag="OO572_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6662203.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(1857..2915)
                     /locus_tag="OO572_003309"
     CDS             complement(1857..2915)
                     /locus_tag="OO572_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662204.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(3281..4621)
                     /gene="fadL"
                     /locus_tag="OO572_003310"
     CDS             complement(3281..4621)
                     /gene="fadL"
                     /locus_tag="OO572_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6662205.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            4993..5277
                     /locus_tag="OO572_003311"
     CDS             4993..5277
                     /locus_tag="OO572_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6662206.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5457..6767
                     /gene="fadI"
                     /locus_tag="OO572_003312"
     CDS             5457..6767
                     /gene="fadI"
                     /locus_tag="OO572_003312"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6662207.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            6767..8911
                     /gene="fadJ"
                     /locus_tag="OO572_003313"
     CDS             6767..8911
                     /gene="fadJ"
                     /locus_tag="OO572_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6662208.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            9114..9599
                     /gene="sixA"
                     /locus_tag="OO572_003314"
     CDS             9114..9599
                     /gene="sixA"
                     /locus_tag="OO572_003314"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6662209.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2483 a         2270 c         2479 g         2672 t
ORIGIN      
        1 cctgctgcag gaaacatcca taccatagtg gcgatcagcc agccggaaat gatcgcgttg
       61 gcaaacatct cgctgggggt gttcttcatc acatccatgc cgattttgac aaatgcatcg
      121 cgagtttctt cattgaagat aggcatatat tcaaatgccc acgcagcaat accagtcccg
      181 agaatattac ccagcagcac gactccccat aaccgcataa gtaagccaac gttgctcatt
      241 gtcggttttt gcatgacggg tagtaccgca gtcacggtgt tttcggtaaa taattgctgg
      301 cgggccatta tgacgataat aaaaccaaag gtataaccga gattctccag caagaagctg
      361 cccggcacac cttccagttc ggcatgaaat atcccttttg ccagtaacga agcgcccatc
      421 gacagacccg ccgcaatggc tgaccacagt agcgccattg cgtcgcgttc tagctctttt
      481 tcaccatcct ggcggatatg ctcatgaatt gccatcgctc gggaggggag tcggtcttca
      541 tctatttcta tttttttgcc gcgctctttt tcttcgctct aaacttcaat ttcgtcgctg
      601 tgttgatcaa ttttgtcgtt gtccatggtc tcttctcttg aattagcacg tatagctaaa
      661 gcgtagcggc ttttttgctc gcaactggcg ggagttactc tgaaaatgta gaaaaggctg
      721 cgtttgcctt tttctgtttc tatagaatca agtagcctac agggcggcga ttaccaggct
      781 atgatcaaat cagcaaatca gggcgtctgg acatcagttg acgtgctgtt acaatcgccc
      841 acacctaaac aggcggatac ggtatcgttc cgtcatggat ggcaaactgc ataagccata
      901 aaaaaacagg gagacattta tgaagcttcg cctgtcggcg cttgctctgg gaactacgct
      961 tctggtgggg tgtgcgagtt ccggtacaga tcagcaaggg cgttctgacc cgttagaagg
     1021 gttcaaccgc accatgtaca acttcaactt caacgtatta gacccgtata ttgttcgacc
     1081 ggtcgctgtc gcctggcgtg attatgttcc gcaaccggcg cgtaatggtt tgagcaactt
     1141 tactggcaac cttgaagaac ctgcggtgat ggttaactac ttcttgcagg gcgaccctta
     1201 tcaggggatg gtccacttta cccgcttttt cctgaacacc attttgggga tgggcggctt
     1261 tattgatgtt gcagggatgg cgaacccgaa actgcaacgg actgaacctc accgcttcgg
     1321 tagtacgctt ggtcattatg gcgtgggtta tgggccttac gttcagttac cgttctacgg
     1381 tagcttcacg ctgcgtgatg acggtggtga tatggcggat gctctttacc cggttctttc
     1441 ctggctgacc tggccgatgt ctgtgggtaa atggacgctt gaagggatcg aaacccgcgc
     1501 tcagctgctg gattccgatg gtctgctgcg tcagtcgtcc gatccttata ttatggtgcg
     1561 cgaagcgtac ttccagcgtc atgatttcat cgctaatggc ggcgaactca aaccgcagga
     1621 aaatccgaac gcgcaagcga ttcaggatga tttaaaagat atcgattctg aataagaaac
     1681 aaataaaaaa ggtgagtcgc aaaactcacc ttttttgttg ttatccctca attaccacaa
     1741 gtaaaaggag ataaaccgac aatgacccgg acgtaatgat gtaaaaatac tgatgtttgt
     1801 cactgactat ttccgatatt caggcgttga aaaatcaaaa aactggaaaa ataattttaa
     1861 atatcatcaa aattaacacc tagtgcagat agttgattaa tttcattttg atatttcgtc
     1921 tggcgatttt gaagtctttt ttcaacttta tcttcgagat tcttaaagtc tttgtattta
     1981 ttttcaggat ggtcaacgtc aactttgtta gcgaataaag tagaaattat ttcttttaca
     2041 ttatcgcgtc taaattttgc atcgtccact tgaaaattat atcttattgc tcgacctaaa
     2101 cgtttaaaaa cttcgttagg ttctctcagt ctttccccgt tgagctcgtt tacaacctga
     2161 tgatcggaac cagttgcaaa tgattctgcc atgatagctt tactgctttc aaaccacgct
     2221 gaagcactcc tggcgttttc actacaatat tgggtgagac tatcaccatt aatacgaatt
     2281 cctctttgaa tgtggtagtc gtcctgatcg tagtaaccga gagataagta atcatttccg
     2341 tttttatttt taattacaat agcgagagag tcgccttttt tatctttacc gtaccaggta
     2401 aataagtcag ttggttcgct tttcccgctc tcatcaaatt ccgttaccgt atgtctgagt
     2461 ttgtgtgata tcttatcgaa gataaaacca ggatctccat catatatttc ttttaatgcg
     2521 ctgaagatca tttccttgtt attgatatca tgagtcttct caactatgtt gtcattaatg
     2581 atagtacgga cgagttcttt gtctttatag ttcgcaggta attctattgg ttcttggttg
     2641 tcgataccaa tagtaatatg gtctgtatca ttggagccaa tatcaatata agtaactgag
     2701 gaattattaa tattagatct ataaggatcg tcgtttactc gtatggcgtt ttttataagc
     2761 tcaagtagat gtttaataca ggtgcttatt ccttccttac cattaaaaag gtttttgaac
     2821 tcttgtacta atccattaat ttcatcattg ttattttcat tgatggattc tgggtaagct
     2881 gcgctggtat tgttgattga aatagatggt agcatgattt tattccatgt tgattgattt
     2941 gttacaaata tatattacct aatgaaatag actatcaaaa aacgctcatt tatttagaac
     3001 ctatctttaa tttgagtgta taagcgcaca atagccaggg gggttgagta tcctggaaat
     3061 gcagaaaagt aaactctttg agaaggacga gcagaacgct tgaaaatatg catttccctt
     3121 caggtttgtc tgtggatacc acttactgat ttgaaagggc aagctgccat caggctgttt
     3181 gcaaaactca ggaggcaagc aatgaggaaa ctactttcgg tgaagtgggg gagttaaagt
     3241 cacctgcata gcaggtgact ttatccaggc gaacgcgtta tcagaacgcg tagttaaagt
     3301 tagtaccgaa cagccaggct ttaccttcag actcgaactg gtatgggcct tcgttaattt
     3361 tcacgctctg accgtgcata taagaaacac caacgtcgac tgaagcatct ttattaaatg
     3421 cgtaagtcgt acctgcactc agccagaaac ggtcctggtc cggaatggag atagaacgat
     3481 tctgagccgg aactgggctg tcatcaaagg cgataccggt acggaaggtc cagttatcat
     3541 cgtagtaata agtggtaccc aacgcgatgc ggtaagcatc tttaaagcct tcatgtttct
     3601 ggaacagcgt gtcgccactg gttgaggtcg ctttcagctg ctggaactga ctccagctgg
     3661 tgtaagccag gctatagtga atcgcccact gtggatcaac acggttataa cctgacactt
     3721 cccacatttc aggcaggttc agcgtcagat aacccgattg cgttgcgcca cctgtcgcgg
     3781 taggaattgg caaaccgtag ttattaaacg cacgattaag atcgctgctg tagttacctt
     3841 tgaagtcaat tttcacttca gaacggtagg tcagtgcata gcggttattt ttatccagtt
     3901 catacaggat tccggcgttc cagccaaagc cccactggtt accgttcaga tgagcgattt
     3961 tggtattact gtcgataccg ttggcggtag ctgccaatgc ttgcccttga ggagtttgcc
     4021 cggcaggaga ttgcataatc tgaccagcaa ccaactgccc cagatcgcct gcgaaacgtt
     4081 caattttcgc gcgagcgtag atggcgttga aaccaagacc aaagctccat gcattattta
     4141 agcgatacgc accgcttaag ttcaggttca tggtttcaag gtcagttgta cccccgacag
     4201 agccgcctgc ataagtatca ttaaactctg ttgccagacc atagttagag gtaatagaag
     4261 cgccccaacc aaattggtcg ttaatcggtg caacaaagtg catgttcgga acccatgccg
     4321 taggcgcgat gttatcggct ttcaggctac gaccagacgg agacgttccg ctgatattca
     4381 catccgggtc aatataaacc gcacccgcag aaaatgtcgg gcggtcaaac atggtaatca
     4441 atgcggggtt acggctaacg ttacctgcat catcggcaat tgcgccttcc cctgaataag
     4501 cccggcccag gccagaggaa gaaaattcgt ttaactgaaa gcctgccgac caggcctggg
     4561 tggagataag tgccactgcg actgcgagag cagactttgt aaacagggtt ttctggctca
     4621 tgaccataac ctcaatgatt tatttttata caaactatgt tacgtgctgt aacaggagcg
     4681 cgaagtgtag ggtctgaggt acatcttaga aatcagacca gtggcgagag tataggtcgg
     4741 accagctaga atgttgcaag tatgtttcca tcttttttca aatatgatct aagaaacgcg
     4801 atccagctgg caaaatttgg aggttgcgca ctcgcggatt cgggtgaatt ttgttttaga
     4861 tcatttttaa gtgtgatttc ggtcacttat ccgattttgc agaattaaca actggagcag
     4921 cactttccgg aagcgtaaaa tatccacaac gtttatctgg caccttgggt gcacatacag
     4981 aggaaatcaa cgatgagtaa atgtagtgct gatgaaaccc cggtttgctg ctgtatggat
     5041 gttggcacca ttatggacaa ctccgactgc accgcgtctt acagccgcgt gtttgcaaac
     5101 cgcgcagaag cagaacaaac gctggcggcg ctgactgaaa aatcccgtag cgtgaaatcc
     5161 gaaccgtgca aaatctcccc gactttcact gaagagtctg acggtgtgcg cctggatatc
     5221 gatttcactt ttgcctgcga agctgaaatg ctgatcttcc agctgggtct gcgttaattc
     5281 ctctcgggta cgtacgcccc tgtcagttgc tggcaggggc gttttctttt ctttcttctg
     5341 tcttcactgt gtcatagctc ccactttcct ttttctccgc ttggctaaat gtaaaaaaat
     5401 ggttaagact atgatcaggt cagaccactt tatttatttt tttacagggg agtgttatgg
     5461 gtcaggtttt accactggtt acccgccagg gcgatcgtat cgccattgtt agcggtttac
     5521 gtacgccttt tgcccgccag gcgacggctt ttcatggcat tcccacggtt gatttaggga
     5581 agatggtggt aggcgaactg ctggcacgca gcgagatccc cgctgaagtg attgaacagc
     5641 tggtctttgg tcaggtcgta caaatgcctg aagcccccaa cattgcgcgt gaaattgttc
     5701 tcggtaccgg aatgaatgtg cataccgatg cttacagcgt cagccgcgct tgcgctacca
     5761 gtttccaagc ggttgcaaac gtcgcagaaa gcctgatggc gggaactatc cgagcgggga
     5821 ttgccggtgg ggcagattcc tcttccgtat tgccaattgg cgtcagtaaa aaactggcgt
     5881 acgtgctggt tgatgtcaac aaagctcgaa ccatgagcca gcgcctgaaa ctcttctctc
     5941 gcctgcgttt gcgcgactta atgcccgtac cgcctgcggt agcagaatat tctactggct
     6001 tgcggatggg cgacaccgcc gaacaaatgg cgaaaaccta cggcatcacc cgagaacagc
     6061 aagatgcatt agcgcaccgt tcgcatcagc gtgccgctca ggcatggtca gacggaaaac
     6121 tcaaagaaga ggtgatgact gcctttatcc ctccttataa acaaccgctt gtcgaagaca
     6181 acaatattcg cggtaattcc tcgcttgccg attacgcaaa gctgcgcccg gcgtttgatc
     6241 gcaaacacgg aacggtaacg gcggcaaaca gtacgccgct gaccgatggc gcggcagcgg
     6301 tgatcctgat gactgaatcc cgggcgaaag aattagggct ggtgccgctg gggtatctgc
     6361 gcagctacgc atttactgcg attgatgtct ggcaggacat gttgctcggt ccagcctggt
     6421 caacaccgct ggcgctggaa cgtgccggtt tgacgatgag cgaactgaca ttgatcgata
     6481 tgcacgaagc ctttgcagct cagacactgg cgaatattca gttgctgggt agtgaacgtt
     6541 ttgctcgtga agtactgggg cgtgcacatg ccactggcga agtggacgat agcaaattta
     6601 acgtgcttgg cggttcgatt gcttacggac atcccttcgc ggcgaccggc gcgcggatga
     6661 ttacccagac attgcatgaa cttcgccgtc gcggcggtgg atttggttta gttaccgcct
     6721 gtgctgccgg tgggcttggc gcggcaatgg ttctggaggc ggaataatgg aaatggcatc
     6781 agcgtttact cttaatgttc gtctggacaa cattgctatc atcaccatcg acgtaccgga
     6841 tgagaaaatg aataccctga aggcggaatt tgcctcgcag gtgcgcgcca ttattaagca
     6901 actccgtgaa aacaaagagt tgcgcggcgt ggtgtttatc tccgcgaaac cggacaattt
     6961 tatcgccgga gcagacatca acatgattgg caactgcaaa acggcgcaag aagcggaagc
     7021 actggcgcgg cagggccaac agttgatggc ggagattcat gctttgccca ttccggttat
     7081 cgcggctatt catggcgctt gcctgggcgg tgggctggag ctggcgctgg cgtgccacgg
     7141 tcgcgtttgt actgacgatc ctaaaacggt gctcggtttg cctgaagtac aacttggatt
     7201 gttacccggt tcaggcggca cccagcgttt accgcgtctg ataggcgtca gtacagcatt
     7261 agagatgatt ctcaccggaa aacaacttcg ggcgaaacag gcattaaagc tggggctggt
     7321 ggatgacgtt gttccgcact ccattttgct ggaagccgct gttgagctgg cgaagaagga
     7381 tcgcccatct tcccgccctc tacctgtacg cgagcgtatt ctggcggggc cgttaggtcg
     7441 tgcgctgctg ttcaaaatgg tcggcaagaa aacagaacac aaaactcaag gcaattatcc
     7501 ggcgacagaa cgcatcctgg aggttgttga aacgggatta gcgcagggca ccagcagcgg
     7561 ctatgacgct gaagctcgcg cctttggcga actggcgatg acgccgcaat cgcaagcttt
     7621 gcgtagtatc ttttttgcca gtacggacgt gaagaaagat cccggtagtg atgcaccgcc
     7681 tgcgccatta aacagcgtgg gaattttagg tggtggcttg atgggcggcg gtattgctta
     7741 tgtcactgct tgtaaagcgg ggcttccggt cagaattaaa gatatcaacc cgcagggcat
     7801 aaatcatgcg ctgaagtaca gttgggatca gctggagggc aaagttcgcc gtcgtcatct
     7861 caaagccagc gaacgtgaca aacagctggc attaatctcc ggaacgacgg actatcgcgg
     7921 ctttgcccat cgcgatctga ttattgaagc ggtgtttgaa aatctcgaat taaaacaaca
     7981 gatggtggcg gaagtggagc aaaattgcgc cgctcatacc atttttgctt cgaacacgtc
     8041 atctttaccg attggtgata tcgccgctca cgccgcgcga cctgagcaag ttattggcct
     8101 gcatttcttc agtccggtgg aaaaaatgcc gctggtagag attattcctc atgcgggtac
     8161 atcggcgcaa accatcgcca ctacggtaaa actggcgaaa aaacagggca aaacgcccat
     8221 tgtcgtgcgt gacaaagccg ggttttacgt caatcgcatc ttagcgcctt acattaatga
     8281 agctatccgc atgctgaccg aaggtgaacg ggtagagcac attgatgccg cgctagtgaa
     8341 atttggtttt ccggtaggcc caatccaact tttggatgag gtaggaatcg acaccgggac
     8401 taaaattatt cctgtactgg aagccgctta tggagaacgt tttagcgcgc ctgcaaatgt
     8461 tgtttcttca attttgaacg acgatcgcaa aggcagaaaa aatggtcggg gtttctatct
     8521 ttatggtcag aaagggcgta aaagcaaaaa acaggtcgat cctgccattt acccgctcat
     8581 tggcgcacaa gggcaggggc gactctccgc accgcaggtt gctgaacggt gtgtgatgtt
     8641 gatgctgaat gaagcagtac gttgtgttga tgagcaggtt atccgtagcg tgcgtgacgg
     8701 ggatattggt gcggtatttg gcattggttt tccgccattt ctcggtggac cgttccgcta
     8761 tatcgattct ctcggcgcgg gcgaagtggt tgcaataatg caacgactgg ccacgcagta
     8821 tggttcccgt tttacccctt gcgagcgttt ggtcgagatg ggcgcgcgtg gggaaagttt
     8881 ttggaaaaca actgcaactg acctgcaata agaaggtcaa agctatatga atccgcgctg
     8941 aatggcggag tgttggtcaa aatgtaaacg catattgact atacttacgc cattgaggta
     9001 aaaaacagcg tttcattcgg tgaatggata aggcacaatg ccggccaccg ttttctttct
     9061 ctggtttcag atgaaagaaa acgggcgaat ctggttaaca aaagcggtgc aatatgcaag
     9121 tttttatcat gcgtcacggc gacgcagccc tcgattccgc cagtgattcg gttcgtcctc
     9181 tgaccactaa tggttgtgac gaatctcgcc tgatggcgaa ctggctgaaa ggtcaaaaag
     9241 tggaaatcga acgcgttctg gtgagcccgt tcctgcgagc cgagcaaaca ctggaagaag
     9301 taggggattg tctgaacctg ccttccagtg cagaagttct gccggagtta acgccctgcg
     9361 gcgatgttgg tctggtcagt gcctatttgc aggcgttgac caatgaaggt gttgcttcgg
     9421 tactggtgat ctctcactta ccgttagtcg ggtatctggt tgccgagtta tgtccgggcg
     9481 aaacgccgcc tatgtttacc acctcggcca ttgccagcgt aacgcttgat gaaagcggaa
     9541 acggtacatt caactggcag atgagcccgt gtaatctgaa gatggcaaaa gctatctgat
     9601 gtgttccaaa ttatgcccaa tgaggtagat tcattgggca taattgctca ttaggcagcg
     9661 gatatctgtc aacctcacta ctatcgaaga ctctccttat ctataaataa aacactttct
     9721 acattcattc ggaaattatt cttctacgcg tatcttgatt ttgcccaaag ttacatgttg
     9781 aaatgaataa tatctgtttg acactcattg tcatttatta atacagataa tagagaaaat
     9841 agagttgaat aatcaggcag gatgtaattg aaacttgatt aaattctggc tatagtgttt
     9901 atat
//
LOCUS       DAJTYL010000126         9790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYL010000126 DAJTYL010000000
VERSION     DAJTYL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.126"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(161..901)
                     /gene="pflA"
                     /locus_tag="OO572_003315"
     CDS             complement(161..901)
                     /gene="pflA"
                     /locus_tag="OO572_003315"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6662210.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="OO572_003316"
     CDS             complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="OO572_003316"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6662211.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3430..4287)
                     /gene="focA"
                     /locus_tag="OO572_003317"
     CDS             complement(3430..4287)
                     /gene="focA"
                     /locus_tag="OO572_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6662212.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /locus_tag="OO572_003318"
     CDS             complement(4693..6453)
                     /locus_tag="OO572_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6662213.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="OO572_003319"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="OO572_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="OO572_003320"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="OO572_003320"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6662214.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..>9790
                     /gene="aroA"
                     /locus_tag="OO572_003321"
     CDS             8567..>9790
                     /gene="aroA"
                     /locus_tag="OO572_003321"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6662215.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKC"
BASE COUNT         2485 a         2340 c         2457 g         2508 t
ORIGIN      
        1 attcgatggg gaagaacttc acgcgagcta ttatcagtcg ttattatctc aggtacggga
       61 tatggtagat gcactataag atgtgttaaa aacgctgtag cagaatgaag cgcggaataa
      121 aaaagcggca actcaataaa gttgccgctt tacgaggaaa ttagaacatt accttatgac
      181 cgtactgctc aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac
      241 catcgagttt gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg
      301 ggagaagttc gattttctca acgttaccca tatcacgggt aaattcaccg aggcgatgtg
      361 ccgaatcgtc atcgtcagac cagcctggga caacaacgta gcggatccac accttcacat
      421 ttttgttcgc cagatattta gcgaactcca gcgtgcggtg gttggaaact ccaaccagat
      481 tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca
      541 gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtatcc agacaggtat
      601 gaatgccttc ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg
      661 cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
      721 ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt
      781 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
      841 tacctgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
      901 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
      961 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
     1021 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
     1081 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
     1141 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
     1201 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
     1261 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
     1321 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
     1381 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac
     1441 agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
     1501 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
     1561 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
     1621 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcattgt taccaaactg
     1681 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
     1741 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
     1801 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
     1861 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca
     1921 gtccatgaag tgatccatgc gctccatcac ttcatcgtag ctcaggagat cgcctttgat
     1981 cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
     2041 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
     2101 gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat
     2161 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt
     2221 cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg
     2281 ccccatagtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
     2341 atcgaggccc ataccaccga tagattcggt tgcccagatt gggtcgccag agaacagttc
     2401 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
     2461 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
     2521 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
     2581 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
     2641 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
     2701 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
     2761 ctgtagagag gtgaactgag cgtatttgtc tttcatcagg tagtcgatac cgtacagcgc
     2821 aacgcgacgg tagtcaccga tgatacggcc acggccataa gcatctggca gaccggtcag
     2881 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
     2941 gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta
     3001 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
     3061 cggagcttca gtctgcagac caacaacttt ttccaacgct ttattgatgt agccagcgtc
     3121 gtgagaggtg atggtggaag caacagcggt gtcaaaatca actggcgcgt gagtgcggtt
     3181 ttccagttta acaccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
     3241 agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga agtcacggac
     3301 gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc
     3361 attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt
     3421 cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac
     3481 caacaaacca ccgccgataa tattaccgat cgtaaccggg atcaggttat cagtgatgaa
     3541 gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc
     3601 cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact
     3661 gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga acgctttgtc
     3721 catcaggctg cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag
     3781 gataccaaga cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag
     3841 gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc acattaaaag
     3901 tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttagccaa
     3961 ctgacaccag gtgatgcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa
     4021 gagatcggct ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac
     4081 cagttttgcc atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga tatagaagac
     4141 gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg
     4201 tttcgttgct ttatagacac ccgcctcttc ggccactttg gccattgcag caggaagtaa
     4261 aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga
     4321 tactaacaaa gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg
     4381 cccgtaactc gcatggtttt tatgcaaata cggagtaact atttgattat ccaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc
//
LOCUS       DAJTYL010000127         9754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYL010000127 DAJTYL010000000
VERSION     DAJTYL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.127"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            383..2014
                     /gene="oppA"
                     /locus_tag="OO572_003322"
     CDS             383..2014
                     /gene="oppA"
                     /locus_tag="OO572_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6662216.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2100..3020
                     /gene="oppB"
                     /locus_tag="OO572_003323"
     CDS             2100..3020
                     /gene="oppB"
                     /locus_tag="OO572_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6662217.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3035..3943
                     /gene="oppC"
                     /locus_tag="OO572_003324"
     CDS             3035..3943
                     /gene="oppC"
                     /locus_tag="OO572_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6662218.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3955..4968
                     /gene="oppD"
                     /locus_tag="OO572_003325"
     CDS             3955..4968
                     /gene="oppD"
                     /locus_tag="OO572_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6662219.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4965..5969
                     /gene="oppF"
                     /locus_tag="OO572_003326"
     CDS             4965..5969
                     /gene="oppF"
                     /locus_tag="OO572_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6662220.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6022..6351)
                     /locus_tag="OO572_003327"
     CDS             complement(6022..6351)
                     /locus_tag="OO572_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6662221.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6386..7846)
                     /gene="cls"
                     /locus_tag="OO572_003328"
     CDS             complement(6386..7846)
                     /gene="cls"
                     /locus_tag="OO572_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6662222.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7989..8162
                     /locus_tag="OO572_003329"
     CDS             7989..8162
                     /locus_tag="OO572_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6662223.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8217..9470)
                     /gene="kch"
                     /locus_tag="OO572_003330"
     CDS             complement(8217..9470)
                     /gene="kch"
                     /locus_tag="OO572_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6662224.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2556 a         2285 c         2428 g         2485 t
ORIGIN      
        1 tttgttaagg catggatgca agctatagat tctgatacgg tcaataaaag agaatcgctt
       61 aacaattttg caaaatgtat tggcgagtaa gaaccgcatt tggtactttc cgggcaaccg
      121 ccagacgatt ctttattggt aatgagaata attaacaatt aaagagcgtc gcgaaagaat
      181 aatgtgtctc gacaggggag acacagtacg aatcgacata aggtgatcgt ctgaatcacc
      241 agaataaata aagtcggtga tagtaatacg taacgataaa gtaacctgac agcagaaagt
      301 ctccgagctt gtgcagggtc ccaatccggg attacacatg ctggttaata ccagtaatta
      361 taatgaggga gtccaaaaaa caatgaccaa catcaccaag agaagtttag tagcagctgg
      421 cgttctggct gcgctaatgg cagggaatgt cgcgctggca gctgatgtac ccgcaggcgt
      481 cacactggcg gaaaaacaaa cactggtacg taacaatggt tcagaagttc agtcattaga
      541 tccgcacaaa attgaaggtg ttccggagtc taatatcagc cgagacctgt ttgaaggctt
      601 actggtcagc gatcttgacg gtcatccagc acctggcgtc gctgaatcct gggataataa
      661 agacgcgaaa gtctggacct tccatttgcg taaagatgcg aaatggtctg atggcacgcc
      721 agtcacagca caagactttg tgtatagctg gcaacgttct gttgatccga acactgcttc
      781 tccgtatgcc agttatctgc aatatgggca tatcgccggt attgatgaaa ttcttgaagg
      841 gaaaaaaccg attaccgatc tcggcgtgaa agctattgat gatcacacat tagaagtcac
      901 cttaagtgaa cccgttccgt acttctataa attacttgtt cacccatcaa cttcaccggt
      961 gccaaaagcc gctatcgaga aattcggcga aaaatggacc cagcctggta atatcgtcac
     1021 caacggtgcc tataccttaa aagattgggt cgtaaacgaa cgaatcgttc ttgaacgcag
     1081 cccgacctac tggaacaacg cgaaaaccgt tattaaccag gtaacctatt tgcctattgc
     1141 ttctgaagtt accgatgtca accgctaccg tagtggtgaa atcgacatga cgtataacaa
     1201 catgccgatc gaattgttcc agaagctgaa aaaagagatc ccggacgaag ttcacgttga
     1261 tccatacctg tgcacttact attacgaaat taacaaccag aaaccgccat tcaacgatgt
     1321 gcgtgtgcgt accgcactga aactaggtat ggaccgcgat atcattgtta ataaagtgaa
     1381 agcgcagggc gacatgcccg cctatggtta tactccaccg tatactgatg gcgcaaaatt
     1441 gactcagcca gaatggtttg gctggagcca ggaaaaacgt aacgaagaag cgaaaaaact
     1501 gctggctgaa gcgggttata ccgcagacaa accgttgacc atcaacctgc ggtataacac
     1561 ctccgatctg cataaaaagc tggcgattgc tgcctcttca ttgtggaaga aaaacattgg
     1621 tgtaaacgtc aaactggtta accaggagtg gaaaacgttc ctcgacaccc gtcaccaggg
     1681 cacttttgat gtggcccgtg caggctggtg tgcagactac aacgaaccaa cttccttcct
     1741 gaacaccatg ctgtcgaaca gctcgatgaa taccgcgcat tataagagcc cggcctttga
     1801 cagcattatg gcggaaacgc tgaaagtgac tgacgaggcg cagcgcacag ctctgtacac
     1861 taaagcagaa caacagctgg ataaggattc ggccattgtt cctgtttatt actacgtgaa
     1921 tgcgcgtctg gtgaaaccgt gggttggtgg ctataccggc aaagatccgc tggataatac
     1981 ctatacccgg aatatgtaca ttgtgaagca ctaatggcaa tacgtggggc aggagtgtcc
     2041 tgctccacgg tgtctgattt ttatcgcatt acagaaggca caggccagaa ggtagggcaa
     2101 tgttaaaatt tattctacgt cgttgtctgg aagcgattcc gacgctattt attcttatta
     2161 ctatttcgtt ctttatgatg cgcctcgcac cgggaagccc ttttaccggc gaacgtactt
     2221 taccgccaga agtgatggcc aatatcgaag cgaaatatca tcttaatgat ccgatcatga
     2281 cacagtattt cagctacctg aaacaactgg cgcatggcga tttcggtcca tcgtttaaat
     2341 ataaagatta ttcggtcaat gacctggtgg catccagttt tcccgtttct gccaaactgg
     2401 gagccgccgc atttttcctt gcggtaatac tgggtgttag tgctggcgtt attgccgcat
     2461 taaaacaaaa caccaaatgg gactataccg tgatggggct ggcaatgacc ggggttgtta
     2521 tccccagttt tgtggttgcg ccattattag tcatgatatt tgcgatcatt ttgcattggc
     2581 tgccgggcgg tggctggaat ggtggggcgc ttaaattcat gatattgccg atggtggcgt
     2641 tgtcactcgc ctatatcgcc agtattgcgc gtattacccg tggctctatg attgaagtat
     2701 tacactccaa ctttattcgt actgcccggg cgaaagggtt acctatgcgg cggatcattt
     2761 tacgccatgc attaaaacct gctctgttac ccgtgctctc ctatatgggc cccgcatttg
     2821 tcggcattat taccggttct atggtcatcg aaaccattta tggtttgccg gggattgggc
     2881 aactgttcgt taatggcgca ttgaaccgtg actattcctt agtgttaagc ctgaccatcc
     2941 tggttggtgc gttaaccatt ttgtttaatg ccattgtcga tgtgctatat gcggttatcg
     3001 acccgaaaat ccgttactga tactggagct cgcgatgatg ttaagtaaga aaaacagcga
     3061 gacgctggaa aatttcagtg aaaagctgga ggtcgaaggg cgcagcttgt ggcaggacgc
     3121 acgtcgacgt tttatgcata accgtgcggc ggttgccagt ctgatagtgc tggtgctgat
     3181 cgcgttattt gtaatcctgg caccgatgct ttcccagttt gcctatgacg atactgactg
     3241 ggcgatgatg tccagcgccc cggatatgga gtccggtcac tactttggta ctgactcatc
     3301 cggtcgcgac ctgcttgtgc gcgttgcgat tggcgggcgt atctcactca tggtcggtgt
     3361 tgctgcggca ctggtggcag tggtcgtggg gacactttat ggttcgcttt ccggttatct
     3421 gggcggtaaa gtggattcgg taatgatgcg tctgctggaa atcctcaact ccttcccatt
     3481 catgttcttc gtcattttgc tggtgacctt tttcggccaa aacatcctgc tgattttcgt
     3541 ggcgattggc atggtttcct ggctggatat ggctcgtatt gtgcgtgggc aaaccctgag
     3601 tctgaagcgc aaagagttta ttgaggcggc acaagttggc ggtgtatcga cgccgggcat
     3661 tgttattcgc cacattgtgc cgaacgtact cggtgtggtg gtggtctacg catcgctact
     3721 ggtgcccagc atgatcctct ttgaatcttt ccttagcttc ctggggttgg gtacgcaaga
     3781 gccgttaagc agctgggggg cattgctgag tgatggcgcg aactcgatgg aagtctctcc
     3841 atggttactg ttgttcccag cgggattcct tgtggtgaca ctgttttgtt tcaactttat
     3901 cggcgatggc ttgcgtgatg ccctcgaccc gaaagatcgt taaggagtgc agccatgagc
     3961 gtaattgaaa ctgcaactgt gccgctcgca caacaacagg ctgacgcact gctgaacgtg
     4021 aaagatttgc gtgtcacctt tagtaccccg gacggcgacg tcacggcggt caatgattta
     4081 aatttttccc tacgtgccgg agaaacgctg ggcattgtag gtgagtctgg ttcgggtaaa
     4141 tcgcaaactg catttgcgtt gatgggcctg ttggctgcca acggacgcat tggcggatcg
     4201 gcaaccttca atgggcgtga aatcctcaat ttgccagagc gtgaactcaa taaactgcgc
     4261 gctgaacaaa tctcaatgat ttttcaggac ccaatgactt cgttgaatcc ctatatgcgc
     4321 gtcggtgagc agttgatgga agtgctgatg ctgcataaga acatgagcaa agctgaggcg
     4381 tttgaagagt cggtgcggat gctcgatgcg gtaaaaatgc cggaagcacg taaacgcatg
     4441 aaaatgtacc cgcacgaatt ttctggcggc atgcgtcagc gagtcatgat tgcgatggca
     4501 ttgctatgtc gacctaagtt gctgattgcg gatgaaccca ctacagcgct ggacgtcacc
     4561 gtacaggcgc agatcatgac gctattgaat gaactgaagc gggaatttaa tactgccatc
     4621 attatgatca cccacgatct tggcgtggtg gcggggatct gtgacaaagt gctggtaatg
     4681 tacgccgggc gcacgatgga atatggcaat gcgcgcgatg tcttttatca acccgttcat
     4741 ccttattcta tcggtttgct caacgcggtg ccgcgtcttg atgcggaagg tgaaacaatg
     4801 ttgaccatcc cgggtaatcc gccaaacttg ctgcgattac cgaaaggttg cccgttccag
     4861 ccacgttgtc cacatgcgat ggaaatttgt agtagcgcac cgccgctgga agagtttacg
     4921 cctggccgtc tgcgtgcttg ctttaaaccg gtggaggaac tgttatgaat gctgtaactg
     4981 aaggaagaaa agtcctcctt gaaatcgccg atcttaaagt gcactttgaa atcaaagatg
     5041 gcaaacagtg gttctggcaa ccgccgaaaa cgctcaaagc cgtcgatggt gtcactcttc
     5101 gcctgtatga aggggaaaca ttaggtgtgg taggggaatc gggatgcggt aagtccacct
     5161 ttgctcgcgc catcatcggt ttggtcaagg cgaccgacgg tcatgttgcc tggttaggta
     5221 aagagttgct gggcatgaag cccgatgaat ggcgtgccgt tcgcagtgat attcagatga
     5281 ttttccagga tccgttggca tcgctaaacc cgcgtatgac catcggcgag atcatcgctg
     5341 aaccactgcg tacttatcat ccgaaaatgt cacgccagga agttcgcgag cgcgtgaagg
     5401 cgatgatgct gaaagtcggg ttattgccta acctgattaa ccgctatccg catgagttct
     5461 ccggtgggca gtgccagcgt atcgggattg ctcgtgctct tattcttgaa ccgaagctga
     5521 ttatctgcga tgagccggtt tcggcgctgg acgtgtcaat tcaggcgcag gtggttaacc
     5581 tgctccagca gctgcaacgt gagatgggat tgtcattaat ttttattgct catgacctgg
     5641 ccgtggtaaa acacatttcc gatcgtgtgt tggtgatgta tctcggccat gcagtagaac
     5701 tggggaccta tgatgaggtc taccacaatc cactacatcc ttacaccagg gcattgatgt
     5761 cggcagtccc catacctgat ccggatctgg agaagaacaa aaccatccag ttactggaag
     5821 gggaattacc gtcgccgatc aacccgcctt ccggttgtgt tttccgtacc cgttgcccga
     5881 ttgccggtcc ggagtgcgcc aaaacacgtc ctgttctgga ggggagtttc agacacgccg
     5941 tttcttgcct gaaagtcgat ccgctttaaa aacaataagg gctgacagtt gtcagccctt
     6001 tttcacgcta aaagcgatta tttattcccg ccagatgata tggcaaagtt tgtgatcttt
     6061 ttcgcggcat aacagaatgc gggcgaaaac atcgctgatt tcaccatctt cactgtccgc
     6121 cagaccaatc accacttcgg caaaaaagtc cgggttcaaa tcgaaatcaa catgttcctg
     6181 ccagtcttcc gccggatcga ataactccgc gccgccgcgc tcttcaaact gaagattgaa
     6241 cagcagaaca tcagctggat cgagattgtc ggcagccagt tcgagaaaaa tatcgtaggc
     6301 ctgctcaagc gtttcatctt cagtcaggcg attgtttaga tccatatcca tagtcactac
     6361 ctgtttaacc tctgttggcg acgttttaca gcaacgggct gaagaagtaa aacagtcgct
     6421 cggcgacacg ttgccataat ggacgtttta gccataaacg ggcatcgagc agacgtgaac
     6481 gcgaaatata atcgtcctga acggcggcaa ggtctgcacc aaaaccttta tcgtcgattg
     6541 ccaaggtaat ctcgaaattc agccacagac tacgcatatc aaggttaacg gtgccaacca
     6601 gacttagttc gccatcgacc agcacgctct tggtatgcag taacccgcct tcaaactgat
     6661 aaattttaac cccagcagca agcagttccg taaagaatgc gcgactggcc cagccgacca
     6721 gcatcgagtc attttttcgc ggaaggataa tactgacatc caccccgcgc tgcgccgccg
     6781 tgcaaatcgc atgaagtaaa tcatcgcttg gcacaaagta gggcgtggtc atgatcaaat
     6841 attcatgcgc cgaataagcc gcagtcaata atgcctggtg aatgagatct tccggaaagc
     6901 cggggccaga agcaattgtg tgaatggtgt gaccgctggc ctgttcaaac ggcataatat
     6961 tgacatctgg tggtggcggc agaatacgtt ttccggtttc aatctcccag tcgcaggaat
     7021 aaataatccc catcgcggtg gcgatggggc cttccatacg cgccatcaga tcaatccatt
     7081 gccctacgcc cgcatcttgt ttgaagtagc gaggatcgac catattcatg ctgccggtgt
     7141 acgcgatgta attatcgatc atgatcatct tgcgatgttg gcgcaggtcc atacggcgta
     7201 aaaacacacg catcagattg acctttaagg cttcgaccac ttcaataccg gcattacgca
     7261 ttagctcggg ccacgggctg cggaaaaaag ccacactccc ggcggagtcg agcatcaatc
     7321 ggcaatgaat accacgccgt gcagccgcca ttaatgattc agccacctgg tccgccatgc
     7381 cgccgggctg ccagatataa aacaccatct caatattatg gcgcgcgagc tggatgtcgc
     7441 ggattaacgc ctgcatcaca tcatctgact cggtcatcag ctgtagctga ttccctttga
     7501 ccccagcgat cccctgacga cgctcgcaaa gcttgaataa tggcgcagcg acactgctat
     7561 tttcttcggc gaagatatgc ttacaggctt taaggtcgtt aagccatttt gcggtggaag
     7621 gccacatcgc tctggcgcgc tcagcgcggc gtttgcctaa atggagctcg ccaacggcaa
     7681 gataggcaat aattccgact aacggcagaa tgtaaataat caacagccag gccatcgcgg
     7741 agggaactgc gcgtcgtttc attagaatgc gtaaagttac gcctgcaatg agcaaccagt
     7801 atcccagaat ggccaaccaa ctcaccaacg tataaacggt tgtcatagat tagaaatcct
     7861 tttgaaagcg cattgttatg agtttacgca tatggattca tctggcaaat aaaaacgcgg
     7921 gaaaagcact ggtcagcgtc gggggcgggg gtataatgac cattctgtta ttgcatagag
     7981 tagttaacat gaagcggagt agaacggaag tggggcgctg gcgcatgcag cgtcaggcta
     8041 gccgacgtaa atcgcgttgg cttgaggggc aatcgcgccg aaatatgcgt atccacagca
     8101 tcaggaagtg cattctaaac aaacagcgta actcgttatt gtttgcgatc tacaatatct
     8161 aaatgtactg ggcaccattg cggtgcccaa taagtttact cttccgtctt taaactctat
     8221 ttttgcgccg attctttact gtcagctttc gtttcttgtt catcgttatc gctgaaaata
     8281 ccatgacctg agttcaacaa cattgaaacg agcatatcat tattaatctc ttcaccattt
     8341 aaaactcgcg ccagaatttc gctgccaaac agttgcggtg agagaatgat atccggatgt
     8401 accatcttaa tcttattcag gtttttgcta tcactgacgg cgagaactgt tttgacatca
     8461 ctgctcatat cttttgccga gagtacaaca aacgcgttat ctgcatcgtt atcactcagc
     8521 gccagaatgg cccggcaacg atcgattccc gctttcttta atactgaact gtcattactg
     8581 tcaccgggga taacatcagc gttatcgcct aagcgttgct caagttgctt gatatcatct
     8641 tcaggcaagt tgctgataac cgttacgttt tgtccgcgtt gattcagttg caggatcgta
     8701 ttgatggcga gaatcgaatg tccgcaaaca ataaaatgat ctttacgatg cattgtatga
     8761 ttgtttcctt ttaaaagttt gttgaatccc ccgcggataa gcggaccaaa aattgaggtc
     8821 atagatgtgg caaaaacggt aatgccggaa ataatgaccg aaatagtgaa caatcgtgct
     8881 gattcagaaa cagggacaat atctccatag ccgacggttg acatggtttc tatcgaaaaa
     8941 tagaacgcgg tcatcaaact ttctattcgc ggattaaaac cttcgcttaa ataaagcgca
     9001 ccgtaggttg agtaaaacag taatgtcgtg aaactaataa atgcaaaaat tgtcccggct
     9061 gcggcgctac tgtgggagaa gtctttgcgc agtatcagca aaaacaccag cgtaaaaatg
     9121 caaaatccaa tactaaattt cagccaggga taaaaatgca gggtgtaaat tagcgctatc
     9181 aacaacaaaa tgatactgat tgcccatgcg agctttgccc ggaacagtaa gccaatggag
     9241 ttcaggacaa gaaagacgcc gagcataaat agtggggcgt ttgccagcaa ggagagatcc
     9301 aactctgaaa atgctttgat atggaaaata tcaagcaaat tgaccgacat accatagatt
     9361 gttttaaaaa taagcaatcc atttaaaaag acggccagcg cgagaatgtc gtgccgtagg
     9421 gtcacccata aatttgttgc tgtttgtttg aatgtagccc agtgactcac tgatacctct
     9481 gtaaccacaa ttttgtttaa ttatcttagc gtgcacattt tctgaacata catgcagcgc
     9541 gtgaatgtgt taaacaatag tagatgactt tatccgcagt aatcaaaggt tttgagtgag
     9601 caactctgcg tcaggaaagg gcttttttcc tgaacatgct cacatcatat aaagaaatac
     9661 agataaagtt attatctgct tgtggtagtg aatgcactga ccggctataa ggaaaggcca
     9721 aacaagaaca cggttgcaaa aaccgtgccc ttaa
//
LOCUS       DAJTYL010000128         9553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYL010000128 DAJTYL010000000
VERSION     DAJTYL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.128"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..189)
                     /gene="torD"
                     /locus_tag="OO572_003331"
     CDS             complement(<1..189)
                     /gene="torD"
                     /locus_tag="OO572_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6662225.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
     gene            complement(186..2726)
                     /gene="torA"
                     /locus_tag="OO572_003332"
                     /pseudo
     CDS             complement(186..2726)
                     /gene="torA"
                     /locus_tag="OO572_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(2726..3898)
                     /gene="torC"
                     /locus_tag="OO572_003333"
     CDS             complement(2726..3898)
                     /gene="torC"
                     /locus_tag="OO572_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6662226.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3985..4677
                     /gene="torR"
                     /locus_tag="OO572_003334"
     CDS             3985..4677
                     /gene="torR"
                     /locus_tag="OO572_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6662227.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(4650..5678)
                     /gene="torT"
                     /locus_tag="OO572_003335"
     CDS             complement(4650..5678)
                     /gene="torT"
                     /locus_tag="OO572_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6662228.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            5761..8472
                     /gene="torS"
                     /locus_tag="OO572_003336"
                     /pseudo
     CDS             5761..8472
                     /gene="torS"
                     /locus_tag="OO572_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            8574..>9553
                     /locus_tag="OO572_003337"
     CDS             8574..>9553
                     /locus_tag="OO572_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6662229.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAV"
BASE COUNT         2213 a         2589 c         2480 g         2271 t
ORIGIN      
        1 cgtggcaata ctgttttcca gatcattcac cgccgcagtg agcggcggtt cgcttttcag
       61 caacgaaaac cattcagcca tctgcgcact ggcgatttgc gtcagttgtt catcgtccag
      121 ttcacgggag aacaactgcg ccagccaggc gtaaacacag gcaatctgtt gtgctgtcag
      181 cgtggtcatg atttcacctg cgacgcggga acatattcgc actgcgccac catctccacg
      241 gggccgttaa acgccgtcac ctgctccact gttccattgt acttctcaat ttccaccagc
      301 gtagtgtgcg cactggtcgc ctgcgccagc tgcgaagtcc cgatgtcgat ggtcagcacg
      361 ttggggttac cgtatttgca cagcgcacca ggctcgccgc ctttatctgg atcgtaccat
      421 gccccttcgt gaattcgcgc cacgccgggt gcatagcggt cagaaactac tgctcctgcc
      481 aacacctgac cgcgagcgtt aaagacgcgt accacatcac cgttacgaat accgcgcgcg
      541 ctggcatcct gcgggttaat gaacactggc tctttacccg ctaccgtata ttgctgacgc
      601 agcgtttccg actcacatag ctgcgagtga agtcggaaat ccggatgcac agattgcaga
      661 tgcaacggat acttctgcga gccgggccca ccgtgggagc gttcgatttt ctcaaaccac
      721 atcggatgcc cctgacaatc gtcgtagttc atatcggcga tggttttcga gtagatctca
      781 atcaggccac tcggcgtgcc cagcggttcg agatccggat cttcgcggaa tgcctggtgg
      841 cgaacaaaca tctgcggatg gtcaaactcg acgtactctt tgttattcca gaagtcatca
      901 aacgctggca gatgaacgcc gcgtcctttg ccttgttgta caccttcctg ccagatgcgt
      961 ttcagccagc ccatttcgtc cagcccttcg gtaaaggctt cttcacgatt aaagcgacgg
     1021 cacagctcgc ggaaaatatc aaagtcgttg cgcgcctcga actacggcgg caccacctgt
     1081 ttcatggcga taatgccacg gttggagtgg ttgccgtact ggtcgagatc gttacgctca
     1141 aactgcgtgg tcgcaggcag cacgatatcg gcaaagcggc aggttgaggt ccactggtta
     1201 tctatggcga taaccgtttc cagcttgcgc cagccttcaa taatgcggtt gatctgctga
     1261 tggcgatgga atgggttagt tccggcaaaa atacacattt tcagcggcgg cagttttacc
     1321 gatttaccgt tccagttgat cactttcccc ggttcgagga tcgcatcgat aaaacgggca
     1381 atcggaatag tgctgctgta gcctttgtag tcactgttgt cgtgaacagg cggaatcgac
     1441 gtagagccgg agaaaccact cagaataacg cctttacgcc ccggcgtgcc tgcgccgttg
     1501 tagtgccagc caaaaccacc acctggcagg ccaatttgcc ccagcatcgc cgccagaacc
     1561 acaatcatcc acgcccactg ttcaccgtgc tgcatacgct gcacgcacca gccagcaata
     1621 atttgcgttc tgttcgccgc catctgccgc gccagcccac gaatggtttc ggcatcaatg
     1681 ccggtcagtt tttcagccca tgcggcatct ttcggctgac cgtctttctc acccagcaga
     1741 tacggcagga actgctcaaa acccacacag tagttagcga ggaagttttt gtcgtacagg
     1801 ttttcactgt acagcgtatg tgccagcgcc agttgcagcg gcacgtcagt ttgcgggtta
     1861 accgcaatgt gcttcacatg ctcgcgcccc agatactcat gggtggatgt gacaaccgga
     1921 tcgatgctga tgacctcaac ttcaccggcg gcgactttcg cttttagctg ctcgtaatat
     1981 tcataaacat cgtgatccgg gcaccaccag ttcgcttgct ggtttttcag caaatcggag
     2041 ccccacagca caatggtttt gctgttctgc aataccagcg gccaggaggt ttgctgttca
     2101 tacacttcca ttgaaccgac tacgcgcggc aggatcacct gcgcagcacc ggtagagtaa
     2161 tctccgcccg taccaacgct attaccatgc aaggcaatag ctttcgccag catccccgaa
     2221 gcgttatgga acatccccgt cgattgccaa ccactggcgg tcagcaaggc actcggcccg
     2281 tgagttttct gtacgcgttc cagttcttca tagaacatgt cgagggcttc atcccagctc
     2341 acgcgcacaa aacggttatc accgcgctgg gaggtgtcgc tcagatggcg cttacgcagc
     2401 cagtccacgc gtaccatcgg ataacgaata cgtgccgcgt tgtgtacgtg atccggcaat
     2461 ccggcgatca ttttcgacgg atatttatcc agttcgaacg gttttgccgc cacaaagcga
     2521 ccatccttca ccatcgcgcg gatagccccc cagtgcgacc cggtaagaat gccctctttc
     2581 gagatgacag cctcagtcgc cgcttgcgcc gcagttgcac ggcgcggcgt taacaatgac
     2641 ggccccagca tcccggcgac ggttaagccg ccgagttgtg ccagaaaacg ccgacgtgat
     2701 gcctgaaaga gatcgttatt gttcattatt tttcttcctt cttatcgccg tgagccttac
     2761 ctgcggtgtc agacgcattc atttgcagat atttcaacaa agtgcgttct tcacgtttat
     2821 cgagactggt aaagccaatc atgccgttga gcgtgccgat ccaaccgtta gcgtcaaagt
     2881 gggcgatttc cggtgcgccg tggcactcgt tacaggtgcc gttgtacaac gaatccgcat
     2941 aggcccagat cggtttgata tcgttcacca tgtcgccttt cttcatccac gcggtggcct
     3001 gcaacttgct ccactcggta ttggtgtcgg caacggtggt tttctccagc gttttcacct
     3061 gctgctgcac atcaccacga atcgaggcaa caaagatgcg tttacctggg aattgggtga
     3121 gtacacgctg acgtccggcg ctttccgtcc agccggtaat ttcaatttgc agccagtcgc
     3181 cgtcacgttt aaggactttc acttccgaag caggcagcag agaaccagag gcttctttat
     3241 cgcctttcgc cgcataaatt ggcttaatat caatagagta cagcgtgtca ccactgtcat
     3301 tagcactggc gcgcagctca tcgaactgct tacggaagcc gctactcata tccggtaact
     3361 ggtgggcaat acctttatga cagtcgatgc aggattgatt atctttcgct gccaccttca
     3421 tctgacgtgc cgcttcagga tgctgcctgg catgatccat cgcatcgtag ttatggcagg
     3481 agcgacaggt tgccgagttg ttttctttca ttcgcgccca ttcacgctcg gcaagttccg
     3541 cgcgtttggc ttcgaatttt tcaggtgtat caatggagtg ggcaataaag gtctggtaaa
     3601 tgtcgttgct cgcttccagt ttgcgcttca ccatgcctgg aatatccggc gggatatgac
     3661 agtcatggca ttcagctcgc acgccggagg cgttctggaa atgcaccgac tgtttatatt
     3721 cttcatacac cggttgcata ctgtggcaac tgacacaaaa ttcggttgtg ctggtgactt
     3781 tgatcccaac gtgtggcaat acaatcagcg caatgccaat cacaatccca attgcgacca
     3841 gcgccagtac cgaccaacga gcactgggtc ggcgtagcgc gttccagagt ttccgcataa
     3901 taacccctgt agaattatgg tttagtgaag cgatcttaat gagcaaatat gaacagatat
     3961 gaacagaatg agtaaaaccc tctgatgcca catcacattg ttattgttga agataagccg
     4021 gttacccagg cgcgattaca atcctacttc actcaggagg ggtataccgt ttccgttacg
     4081 gcgagcggtg ccgggctacg ggaaattatg cagaatcagc cggtagattt aattctgctg
     4141 gatatcaact tacccgatga aaatggcctg atgttaaccc gcgccctgcg agaacgctca
     4201 acggtgggga ttattctggt taccggacgc agcgatcgga ttgaccgtat tgttgggctg
     4261 gaaatgggcg cagacgatta cgtcaccaaa ccgctggaac tgcgcgaact ggtagtacgg
     4321 gtgaaaaatc tgctctggcg aatcgacctc gcgcgacaag ctcaaccgca cactcaggac
     4381 tactgctatc gctttgcggg ttattgcctg aatgtgtcgc gccatacgct ggagcgggat
     4441 ggcgagccga ttaaactgac ccgcgcagag tatgaaatgt tggtggcatt tgtgactaat
     4501 ccgggcgaaa ttctcagccg tgagcgtctg ctgcgtatgc tttctgcgcg tcgggtggaa
     4561 aaccctgacc tgcgcaccgt cgatgtgtta attcgtcgtt tacgtcataa actcagcgcg
     4621 gatttactgg tgacgcaaca tggtgaaggt tatttcttag ccgctgatgt gtgctgataa
     4681 aaatagaccg gacgaaatcc ccccggtgac agcgagcggc ggatatgttc tcggtcggca
     4741 tttttcggcg tcagaactaa aatcggtggg ctgacattat cagacaccga ttgcccctgt
     4801 aattgcctga tggcctgctc aactgccagt tccccctgcc agaccatttg atcgctggca
     4861 gccataatca ctcttccccg cttcagcccg cgatacacct gatgtgaaag ataaaacgac
     4921 accacggtaa gcggcgtttt caggttacgc ccttcaccca ttgccgcctc tgccgcaatg
     4981 gccgttccgg caacgacgtc gatttctggg tggcgttcca gcatctcctg caacaggttg
     5041 cgctggattt caatatcgtt atcaccaagc gcaatatcaa caatacgcac cgggcttccg
     5101 gcaatggctg cgcgaaaacc ctcgaccatc tccttactgc ccccggcgtt atcgggtccg
     5161 ggcatcagca gcacgttcag tggtttaccg tgcgcccatt gcaccaaata tcgcccaggt
     5221 tgatagccca tctgaaacca gggtacacca acgcggcttt tcacctgggg atcatcaaga
     5281 gcatttacca gttcgatcac cggcagattt gctacctgct tttgcaggtc gggaaatgac
     5341 gtcgtgctac taccgagcaa aatggcctct gcgccccact gtttacactg gtcgatttgt
     5401 gcttgctggg tagccaactg gctgtagccg cctgcctcca gcacttttaa atccacaccg
     5461 tagcggcgag cagcctcctg cataccatag ttcaacgata accagtatga atctttcagg
     5521 ctgggataaa gcgcgcacag tttccatacg cgtttggctt taagcggcat agaggcttgc
     5581 accgtgaaat gctgcgcatc atgccagcgc aacaggttat cagccgaaaa tgccgacaac
     5641 atgaaaaggg aaagaagtaa aaatagcagt acgcgcatga tagcctcatc aataataagg
     5701 ctttatgcta gatgcattcc actttgcgac tcaacctttt tcaccttaag tgcaccgacc
     5761 gtgaatttaa ccctgacccg aagactctgg atgggctttg ccctgatggc gctgttaacc
     5821 ctgaccagta ccctggtggg atggtacaac ctgcgcttta tcagccaggt ggaaaaagac
     5881 aatactcagg cattgattcc taccatgaat atggcgcgcc agttgagcga agccagcgcc
     5941 tgggaacttt tcgccgcgca gaacctgacc agtgccgata acgaaaagat gtggcaggcg
     6001 caggggcgaa tgctcaccgc ataaagcctg aagattaatg cgttgctgca agcgttacgg
     6061 gaacaaggtt ttgataccac cgctattgaa caacaggagc aggagatctc ccgttcatta
     6121 cgtcagcaag gggaactggt ggggcggcgt ctgcaactac gccagcaaca acggcaactc
     6181 agtcagcaga tagctgccgc cgatgagatc gcacgcctgg agcaaggtca ggcgaataat
     6241 gcgacaactt ccgctggagc gacccaggcc gggatttacg atttgatcga acaagatcag
     6301 cgtcaggctg ctgaaagtgc actcgatcgg ctgattgata tcgatcttga gtatgttaac
     6361 cagatgaatg aactgcgcct tagcgctctg cgggtgcagc aaatggtgat gaatctgggg
     6421 ctggagcaga tccagaaaaa tgcaccaacg ctggaaaagc agctcaataa tgcggtgaaa
     6481 attctgcaac gtcggcaaat acgcattgaa gatccgggag ttcgtgcgca ggtcgcaaca
     6541 acgttaacta ccgttagcca atatagcgat ctgttggcgc tgttccagca ggacagtgaa
     6601 atcagcaatc acctgcaaac tctcgcccaa aataatatcg cccagttcgc gcagtttagt
     6661 agcgaagtca gtcagctggt cgacaccatt gagctgcaca atcagcatgg actggcacat
     6721 ctggaaaaag ccagtgcacg cgggcaatac agcctgttat tgctggggat ggtttcactt
     6781 tgcgcactga ttatgatcct ctggcgcgtg gtttatcgct cattcacgcg tccacttgcc
     6841 gaacaaacgc aggcgctgca acggctgctg gacggtgata ttgactcccc tttcccggaa
     6901 accgctggcg tacgggagct ggataccatc gggcggctga tggatgcgtt tcgcagcagt
     6961 gttcatgcac tgaatcgcca ccgcgaacag ctggcggcgc aggtcaaagc gcgtacagct
     7021 gaattgcagg aactggtgat agaacaccga caggcacggg cggaagcaga aaaagccagc
     7081 caggcaaaat cggcgtttct gacggcgatg agccatgaga tccgcacacc gctgtacggt
     7141 attctcggca ctgctcaact gctggcagat aaccccgcac ttaacgccca gcgtgatgat
     7201 ttgcaggcga ttaccgactc tggcgaatcg ttgctgacca tcctcaacga tattctcgat
     7261 tattcggcta tcgaagcagg tggcaaaaat gtttcagtca gcgatgagcc ctttgaaccg
     7321 cgcccgctgc tggaaagtac cctgcaatta atgagcggac gggtgaaagg tcgcccgatt
     7381 cgcctggcaa cggcaattgc cgatgatgta ccgagcgcgt taatgggcga tccacgacgt
     7441 attcgtcagg tcattaccaa cctattgagc aatgccctgc gttttactga cgaagggcac
     7501 attatcctgc gtagtcgcac tgatggcgag caatggctgg tcgaagtgga agacagcggc
     7561 tgcggtattg ctcccgcgaa actggcagaa atcttccagc catttgtgca ggtaagcggc
     7621 aaacgcggcg gcaccgggct ggggctgact atcagtagcc gtctggccca ggcgatgggc
     7681 ggcgaactga gcgccaccag cacgccggag gttggaagct gtttttgttt acgcttgccg
     7741 ttacgtgttg ccactgcacc cgtgccaaaa acagccaatc aggcggtgcg tcttgacggt
     7801 ttacgtttgc tgttaattga agataacccg ctaacccagc gaattaccgt tgagatgctg
     7861 aacaccagtg gtgcgcaggt tgttgctatt ggcaatgccg cgcaggcttt agagacactg
     7921 caaaatagcg aaccgtttgc tgccgcactg gtggattttg atctgccgga tatcgacggc
     7981 attacccttg cccggcaact ggcacagcaa tatccgtcgc tggttttgat tggctttagc
     8041 gcccatgtca ttgacgaaac gctgcgccag cgtaccagtt cactatttcg cgggattatc
     8101 cctaaaccgg tgccgcgtga agtgctcggt cagttactgg cgcactatct ccaactgcaa
     8161 gccaataacg atctaccact ggatgtctcg caactcaatg aagacgctca gttaatgggg
     8221 acggagaaga tccacgaatg gctggcatta tttaaacaac atgccctgcc gcttctcgat
     8281 gaaatcgaca ttgcccgcgc cagccaggac agcgagaaaa taaagcgtgc cgcgcatcag
     8341 ctaaaaagca gttgctcgag tctgggaatg cgtagcgcca gtcagctatg cgcacaactg
     8401 gagcagcagc cattatctgc cccccttcca cacgaagaaa ttacacgcag tgttgccgct
     8461 ctggaagcat gattgaataa gaaagacctg aacgcgattt gaatcaatat cagccagtta
     8521 agaataacta gaatagtggc atttggtatg caacttaacg gactggcaaa atcatggcag
     8581 agaaaaaaag aacccgctgg cagcggcggc caggcacgac gggcggcaaa ttaccgtgga
     8641 atgactggcg caatgccacg acctggcgta aagcgacgca attcttacta ttggcaatga
     8701 atatttatat tgccatcacg ttctggtatt gggtgcgcta ttacgaaacg gcaggtagta
     8761 cgacatttgt cgccagaccg ggaggtatcg aaggctggct accgattgcc ggtctgatga
     8821 atctgaaata tagccttgca acaggtcagt taccgtccgt ccacgccgcc gcgatgctgt
     8881 tattggtcgc ttttatcgtc atcagtctat tactcaaaaa ggccttttgc tcatggttat
     8941 gcccggttgg tacgctttct gaattaatcg gcgatctcgg taacaaactg tttggtcggc
     9001 aatgtgtcct tccccgctgg ctggatattc ctctgcgcgg cgtgaagtat ttgctgttga
     9061 gtttatttct ctatatcgcg ttattgatgc ccgctcaggc gattcactat tttatgttgt
     9121 cgccctatag cgtggtgatg gacgttaaaa tgctcgattt ctttcgtcat atggggaccg
     9181 cgacattaat cagcgtgacc gttttgctga ttgccagcct gtttattcgc catgcctggt
     9241 gtcgttatct ttgcccatat ggcgcgctga tgggcgtggt ttcgctattg tcgccgttta
     9301 agattcgtcg caatgccgaa agttgtatcg attgtggcaa atgcgcaaaa aagtgcccat
     9361 cacggatccc ggtagataaa ttaattcagg tacgaacagt ggaatgtacc ggctgtatga
     9421 cttgcgtaga gtcatgtccg gtagcctcaa cattgacctt ttcactgcaa aaacctgcag
     9481 caaataaaaa agcctttgcg ttgtctggct ggttaatgac gctactggtt ctggggatta
     9541 tgtttgcggt gat
//
LOCUS       DAJTYL010000129         9527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYL010000129 DAJTYL010000000
VERSION     DAJTYL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.129"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(320..526)
                     /locus_tag="OO572_003338"
     CDS             complement(320..526)
                     /locus_tag="OO572_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6662230.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(633..1196)
                     /locus_tag="OO572_003339"
     CDS             complement(633..1196)
                     /locus_tag="OO572_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6662231.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(2571..4406)
                     /locus_tag="OO572_003340"
     CDS             complement(2571..4406)
                     /locus_tag="OO572_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662232.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            4507..4794
                     /locus_tag="OO572_003341"
     CDS             4507..4794
                     /locus_tag="OO572_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6662233.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            4766..6295
                     /locus_tag="OO572_003342"
     CDS             4766..6295
                     /locus_tag="OO572_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6662234.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(6465..7682)
                     /locus_tag="OO572_003343"
     CDS             complement(6465..7682)
                     /locus_tag="OO572_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662235.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8049..9035)
                     /locus_tag="OO572_003344"
     CDS             complement(8049..9035)
                     /locus_tag="OO572_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662236.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9032..9527)
                     /locus_tag="OO572_003345"
                     /pseudo
     CDS             complement(9032..9527)
                     /locus_tag="OO572_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2989 a         2196 c         1761 g         2581 t
ORIGIN      
        1 gggaaacagg taatgatatc cccgttgtca atcacaggag ggtaatggag atccccccgt
       61 attgctgtta ttctggaagt gtttttaata ttcgtattga tagttctcag agatatatca
      121 cactctagat attttaatta agtatcagtt cgatatcggc atattgtaca ctacctcagg
      181 tagatgatga gatcagatac agggatcaac tgacggtgag cgcagtcccc acattttatc
      241 cagggcttac tgcatattcc caacagccat tcgttagcca ggtagacgaa taccctgatt
      301 tgccgctggt tttgctttcc tatcgaaggg cccatacatc atcacgccca cgaaacaacc
      361 gacgaaataa cgcaactttc tcatccgtgg ataatacgaa aacgcactgc acagggctct
      421 gcggtttacg tcgccattca atcccatgag cttcaagtaa tgagaccagc cgggtatttt
      481 ctgcccgcag tactgctagt tcatcgctgt cagatttata aaccatatgc gttatcactt
      541 gtacctgtct cccggcggca ccacgactga aattcgtgtg aaggcaaatc cacgcgataa
      601 cttacgatag ttcggacata agcccccata attcagttat gtcgtggtcg accccggtta
      661 cttttttcgg gaagcctgct acggcccaga ataataccgt gttctttctg atcctgttta
      721 gccagatagc taagggcgta acgtaagtcg tctaccgcag atttatcact atagtgaatc
      781 acgtgatcga tacgcaccgg gtatttgtct ttagcccggc acagatgaaa ataaccctcc
      841 ccttccgtga tccgcctcca gatatctccc agttgcctgg atatctgata agagttcttg
      901 tgacgttgtc catccaggta gccgataaaa tgaatatgaa gccctttgtc tggtgtatat
      961 tccattaccc agtaatatcc cgccaacatc gtctgggttt cgctaagtaa acggtatatt
     1021 tccatacaca tactgtgctt acaggaatgc ccgaaactgg gcgtgtcttt cctgtaagca
     1081 aaatcaattc tgaaaggtaa cagtttagaa aaacgttgaa acataccatc catgtgttca
     1141 ttcacgtctt tcagaatcat gaaatccatt ttgtaattgg gattggcatt atacatttta
     1201 tagtccttac tttataaaac tcacaggaag agagaattac gtaaatactg agagtagaga
     1261 aacgccgtac cacggcatta ataaacgaag taaaccagca gcacagacaa cctgataaaa
     1321 ctgttttgta ttaccgcatt actgtgatga ggtaatactt cagaatacag tgagactctc
     1381 ttaaactgag aaccagtacg aattacaatg gaaaaacaca cctcatagaa ttgagtttca
     1441 ggaagcacca ctaaattctt tacaggaagt aatctgacat tcacaaaact cagtatcggc
     1501 tagctacagg caacatcgct attaatattg tcgaaccatt aagtaaagcc gagagagacc
     1561 acgggtattt aatcattagc ataaaagaat aatggtatac agaacatacc cacacaccat
     1621 aagaagattg ttccatacag aaaagatcat atttacctat acctataaac tgtaacggag
     1681 tcataacacg ctctaatagt gttccgatac gtaaccagtg ttaagtacgt acaggacaac
     1741 attacacaca taattcacca tacagcttcc cccatatgct tcactgcgta gtgtatggag
     1801 atatatgcat tacacacata cagaatactt agcataaaga ggagtgacat taccacgtaa
     1861 agagataagt tatacaacaa acatacaaac aatacggcac agacacacaa taccataact
     1921 ccaactaata atactaatta accttaatta tttatggtat atgaaataaa tgtgattctc
     1981 ccggaactaa ctaacctaac cagacagatg atcactgtat aaaaacttca gttaatactc
     2041 atttgttttg tcgactcagt atataatgag tgaccagtaa tcaggataat cacacttaca
     2101 taacctgtaa aaaagaacaa gaagaataag ccactaacat aatgcaaaat ggcatctgaa
     2161 tcgatcgcag tttggcattc gaatcgatca tgatttggcc caatccgtga tcgaatagag
     2221 cggaatacgc aacataattc accagacaag agcttacacc gaattacaga ttatgtatta
     2281 atgtcaaaac tggcatattt tacagataca atgaacatgc aacccatcaa tctaataggg
     2341 gcggtatgta catctgccca tgaatgggat ggcgctaaga atacaaaaca attagacata
     2401 gatcacaaaa acacaccatt ctaattgaaa aacaaaacaa acaaataaat catactatta
     2461 tagaattttc tcttgccaga catagataat acccctgcta attttgctaa tggttagcaa
     2521 aattagcagg gtatataaag attattgacc caaaacacac tcagatgaat tcacgacagc
     2581 tcaggagggt tattcctgat gtcatcttca caaataatgg ttaacgcatc atatatcatt
     2641 ctgcgtctgt tcctgttttc ggccttatcg ccatacaaca tcatcacatt tttaatttca
     2701 tcgaccggtg atttcacata gtgaactaat acaccaagct tctcaacaca ttgcagtaaa
     2761 gtatatttct tactgcctgt gaaaagccct atcatagttt ctttacgttc gcgactggat
     2821 aatactttgt tttccataat gacttctgcc agcgtcttac catcataaac atactcttta
     2881 tctatcagaa tatttatctt atccagcacc ttctctacgt catcaagcaa acctgtaata
     2941 tatgggggcg caatatatgg tttcgtactg atcttttcaa gcatacaatt atacatctta
     3001 ataacacgca tcagggtaaa attgttcgca tcatactgta tttcaccctc cttatataca
     3061 ggatcaataa gtggtatttt ttcaataaat gcatcctgat aggcttttac atggtcaagc
     3121 caataaaaca acgagccata ctttatattt tttctattta ctttaacctc cagtgctgtc
     3181 cgaagcaacg ataatgcccc cctgacatat cctgaaggta attcaccaaa caatcctttt
     3241 ctttccatgt catcataatg ctgcagcaca tcattaattt tatttttcct tatcagacaa
     3301 atgtacaccg caagtgtttc gtagcagctg gtcacatgaa cagcctcatt ctgcaactgc
     3361 ccaaaatagc ttataatctt tttcagatat gcgtcatcac aaccattctt atcattaata
     3421 ttaaatacaa gatctttcag acgggaaata tattcctgag atttatcatc aagtgaaaca
     3481 ctataacgag acaatgttcc ttctgcactg ttttgaaact tataaaacat ttccagtaat
     3541 ttttttgcat aaggcgttgt atcaaagctg gtaaagtcat aaagatattg cgcagcgtaa
     3601 ccatacaaac catgaatcca gagtccttct ctcaccaaca ctctgagata atatgcaagg
     3661 ctttgaagta tcttcgtctt tcgttgtata tcaagtaaga cacttaatga ataaaatgca
     3721 ttatagaatt tatcaatatt cctgagcact ttctccagtt catccttatc cagcttcttc
     3781 tcccggtaat cctttttata ttcatttttg atagccatga tatattgttg catcaggctc
     3841 ttgcttttcc cgttatcctt ccgtatagtt cctgtatatt tccccagagc atacagcttt
     3901 ccattttcat gaaaatcttc cttgttgaat cccagttcag ccagatattc ccgaagataa
     3961 cgtttaacag cagcttcatc gctttctgta tcagcgtgat caagacaagg acaatacgat
     4021 ttctgtaaaa aattataaat atgataataa aaagcagagt cattctctgg tctgtatgta
     4081 ttatacatat ctacaacaag aaaaataacc ccaaaagtgc gcagacaata gctgcgaagg
     4141 aattcaggtt ttagtgtgga atgcactgga taagaattca tcatgtaata aaaatgttca
     4201 tatcgggcat atatgatccg ccgttgctcc tgactgaaca ccccttcagc atcaataata
     4261 cgactgatct cattataacg ttgatcccag aatttttgat gcatttcaaa attggtactc
     4321 ttccgcttgt gtgccggatt ctcgccttcc ttaaacaacc ccaaaacagt acaaatatca
     4381 tacaacatat ttgtaaatgt gcgcatatgc tgtgctctcc ggttggtttg cattttatcg
     4441 cagaggacat tctgagctta acgtatcaaa ctgacacaac gacatgcaat gcatatggag
     4501 aacaatatgt caaatcaaac cggacagctt tacacgcctt cacgcatcct gcgtatggct
     4561 gaacttaccc gactgctggg tatatcaaga tctactattt acgtgaaaat aaatccggcc
     4621 tcgaaatacc acgaccctac ctttcccaaa ccaataaggc ttggtagtgc atccgtaggc
     4681 tggcgggcat ctgccatcga tgagtggctg atgttacata cggcaccagc ctggtctgaa
     4741 cctgaaaaca aactaaagga acttaatgat gacgacaaaa ctatcactcc ctgaacttga
     4801 cacaactaaa ctgcctgaca ggtgccaggc attacttgat gaaatgcatg aagaaacagg
     4861 aatacgccgt gaaatacttc tgtctgttat gctgacggtc atggctgcct cggtccagga
     4921 tacgcatgaa gttgaacttt ccggagggca acgtaccagc cttcagatat ttatgtgtct
     4981 ttcatcagca tcaggcagtg gaaaaacctc tgcctgcgca aaattaatcg ctcctgttca
     5041 tgaaacagaa gaagaactac atcaggcata tatcgacgat aaaaaaaact atgaccgaat
     5101 gatggaaatg tggacaacag ataaaaaaat cctggagcgg agatataaaa aggaaattga
     5161 aagatccccg gaaaatgctg ctgctgcacg agcagcactt gaaaagtgta tagcaaataa
     5221 acccgaacct cccgtacaac aggttctcat cgtgaatgac gcgacaccag aagggatagc
     5281 tttgaaactc agccagtcac cctctcttct gttgctgtct gatgaaggag gtactattct
     5341 ggataagcgt ttcgaacgca aatcggcact gtacaacaca ttatggagtg ggcaaccagt
     5401 caacgttgaa cgggcatcca gacccgggtt ccggctgaat gatgtccgtc tcactatact
     5461 tatactgact caaccggtaa tatttaataa gttttttact ctcactggcg atcagatccg
     5521 gggcaatggc tttctggccc ggttgctatt ttgcgaaccc ggagcaatta aaataatgac
     5581 gacagaaccg catactgcca ctcctgttgt gacgcaacaa tgtgcaagct gttttcgtaa
     5641 gaaaagtttt ggcagtcaga tcagagactc tctgagagca tccagagaaa gacgtgcaaa
     5701 aagtaaagag cgcatctgca tgacattatc aaatgcagca tcccgcgcac tggagcgctt
     5761 tcacaaagaa aatatggata ctgtcagaca gaatcctcat atgggtacat ttgaagacat
     5821 tattgtcaga aaaagagagc aggttgtcag gatagcagca cttctggaac tggagaaaga
     5881 tccagactcg acagtaataa cgctggaaag tactaatagc gccatttatc tcattgaatt
     5941 ttatttcaag catcttatat ataaacttga atcccttcgg gaaatatccc cggctgagaa
     6001 acttgataaa tggcttcaga aaagaattat cacaacagca ggttacatat tccagaaaag
     6061 ttatatttta caatatgccc catatgctct caggaaaaaa tgtgttctgg atgaagcact
     6121 tgacatactg gcggaacaaa ggaaaataag gattgatgat aacctggttg tttatatagg
     6181 aaacacgatt acaccatccg aactggcaaa aaaactaaat attcctgcat tcgatgccgg
     6241 agttttcatt tgtgatcatc aaaacatact gaagtatcac cgaaacagac tatagatact
     6301 atcggacaaa aaataaatta tcacaagtgt attattcgtt ataataatga ataatcgtta
     6361 tctgagtgca atgccaggtt ttcattacga actggcattg catttatttt tcaaaaaaga
     6421 aagcagtaac catttggcta ctctgagtat gccagtccat gaaactaagc gaattcctta
     6481 gtgtttttag caagttgaga catcacataa tccgcccatg cctgcatcat cggtacacgc
     6541 tgctccagta gatcagtacg atgatatgcc gcctcaacct tatttttcag cgtatgagcg
     6601 agcgcccttt ccgccagatc ccgcgaatac ccctgttcac tacaccagtc cctgaatgtt
     6661 gagcgaaaac catgtgccgt ggcaactcgc cccggaatgt cactgacggc tttcttttta
     6721 cgcagaaaac ttgtcaacac catatcggaa aggatctgct gctttctggg tgaagggaac
     6781 accagttcat catgcaggcc acgtatattt tccagaatgt aaatagcctg ccgggataaa
     6841 ggaacacgat gctgtagcct agctttcatt ctttctgcag gtatagtcca tacccgctta
     6901 tgaaaatcaa tttcagccca gcgcattccc ctggcttcgc ccgagcgagt tgctgtaagt
     6961 atcaccatta ataacagtgc gcgggtaaca ttataaggtt catcactata cacactggtc
     7021 gccacaaaaa gcggtaactg cctccagggc attgcgggtt ggtgttcatc acgtcctctt
     7081 gtctgctgag gaagcaaatg gtcaaccaca tcaacaggat ttgccacaca aaaaccgtgc
     7141 gcccatcccc actgcataac aacatgaatg cgctgtttaa cccggcttgc cgtttctgac
     7201 aaggttaacc agactggacg cagtgtttct gccacgtccg cagccgtaat cgaatccagc
     7261 gtttttgctc ccagttgagg aaacgcgtaa ttctcaagcg tcgataacca ctgccttaca
     7321 tgctttggat tctcccatcc aggagacagt tctgcatgta cacgcctggc tgcatcggca
     7381 aatgttggga taacgacttt ctcagattca gcctttttaa tctccagagg atcatcacct
     7441 gcagcaagtt gctctcgcat tatccgggca gtacgtgcag cttcagcaat actgacctct
     7501 gggtaagttc ccaatccagc attacgtctt ttttgtgtca ccggacttac ataacgaaaa
     7561 acccatttcc cccgcccctt tactgaagaa ggatgaaggg tcagtccggt aattccccca
     7621 tggggcaatg gtttgtcatc aggtttgata tgtcttgctt tcgtatccgt caatactgcc
     7681 atacgctaat tcccgtcact ctggtatgcc atccagtatg ccattagtcc ctcgcttcac
     7741 taagaataca ttagataaca tcggacaaca aaaagtgtaa tacattgttt ataaagaaat
     7801 tatataacac ccctggatta agtcagattt atttcaggcg gtccccctca ccgccatatt
     7861 taaagaagag cgcgtacgaa agtacgggct tttttttcgt atattgtaca caccgggtgc
     7921 ccccgcccac gtctgttagg gcgaggggaa attgtgcaca tcaactgtct ggttactcag
     7981 tcaacaatca aaatgcgtaa gatagtacag aacatatatg cgttatctgg tcacttcctg
     8041 taacttcctc acttattaaa atgcccccaa cggaaataca tgtcacatgc catcataaaa
     8101 tcgttatgcc agaggaatcg acaatggaaa aaatagtaaa ttaagcacgg tacatagcag
     8161 agtaaaaaat aggcttcaaa ccaaatccgc cattccacaa gcgttgccaa taccgacacc
     8221 agcgtaatcc acatcatttc aataacccgc aatatgaata accgaagcca aagcggcttg
     8281 gcaaaaaagg tcgacaacgt attttcactg gagaaaaaca gatcgatttc ctgcaaaaaa
     8341 aacctgccca gaaaagcggg ccgcttggag aagcgcagaa aaaacagatt aatcgccaac
     8401 actacgccac aaagaatgag caatgcaatt atcgatttaa acgttaacca ctgcccctct
     8461 gtcacaaaag aatcactgtt acgcagcggg ctgatagtaa gcaggttaac aaccgttgag
     8521 atgcacaccg aaagcagcac atagcgagtc agacttttat attttacttt tttcgctact
     8581 gaaatagcga cctgcttgac ataactggtg cggatcatcc gaaacagcag aaagatgacc
     8641 aacggcccaa gtaacgaaat caggaacagc gcatataacg agggtagaag ctggtatagc
     8701 ccccaactca ttgcactcaa catgattaac gacagaaatc ctgccaccag aggttgtgta
     8761 agaaataatt ctattttgtg gttctgcttt ttcttaaacc acgaaaaatc gatattcttc
     8821 gtgacgcatt tataagccca gtacgccttg agatcaaaaa taaaattata cagcaccagg
     8881 gccagaatcc cactaactac accaatatcc tctgatggaa aagtaaaccc tgcatttttc
     8941 caataaaagc ctatcagaat aagtccaata gcataaatgt atatcagatt tcgtttatcc
     9001 tgaaagcgaa taaaagttaa ataatcagga atcatttgaa tacctttgca tcactaaatg
     9061 ctttacgaca atgcataact cgttgctgat ccacgccagc aaaatcattt aacatattcc
     9121 ccaaataggg cttattactt atttcaagga aagctaccgg ggttggcata ttcagaatat
     9181 gtatcgagag attcagtcca tttcaccgta tgagttaagt gtaatgagag attttctttg
     9241 ataattgacg gagcgacctc gctttttgct gttacgttca tcagaacctg atgctctggc
     9301 gaagcgatat ccagtccggc aagataatcg cgcattgcct gaacgccgtc ttccatcaaa
     9361 cgcgtgtgcc aggcaccgct cacaccgagt ttaaccggtt cgtatccagc ggccatcagc
     9421 agcgtggtaa attcattcaa cgcagcctgc gttcccccaa taacctgctg gcgcggcgtg
     9481 ttatcacagc taatatccag cgcaatgcct gattccgtaa tcatcgt
//
LOCUS       DAJTYL010000130         9497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYL010000130 DAJTYL010000000
VERSION     DAJTYL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.130"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..661
                     /locus_tag="OO572_003346"
     CDS             <1..661
                     /locus_tag="OO572_003346"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6662237.1"
                     /translation="ITADGQGITLVNIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGL
                     RESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGEEVK
                     QRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGTLLC
                     VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPF
                     R"
     gene            1004..1720
                     /locus_tag="OO572_003347"
     CDS             1004..1720
                     /locus_tag="OO572_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6662238.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2176..2251)
                     /locus_tag="OO572_003348"
     tRNA            complement(2176..2251)
                     /locus_tag="OO572_003348"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2216..2218),aa:Asn,seq:gtt)
     gene            complement(2352..3979)
                     /gene="yeeO"
                     /locus_tag="OO572_003349"
                     /pseudo
     CDS             complement(2352..3979)
                     /gene="yeeO"
                     /locus_tag="OO572_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3984..4059
                     /locus_tag="OO572_003350"
     tRNA            3984..4059
                     /locus_tag="OO572_003350"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4017..4019,aa:Asn,seq:gtt)
     gene            complement(4097..5047)
                     /gene="cbl"
                     /locus_tag="OO572_003351"
     CDS             complement(4097..5047)
                     /gene="cbl"
                     /locus_tag="OO572_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6662239.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5149..6066)
                     /gene="nac"
                     /locus_tag="OO572_003352"
     CDS             complement(5149..6066)
                     /gene="nac"
                     /locus_tag="OO572_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6662240.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            6394..6469
                     /locus_tag="OO572_003353"
     tRNA            6394..6469
                     /locus_tag="OO572_003353"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6427..6429,aa:Asn,seq:gtt)
     gene            complement(6525..7460)
                     /gene="ldtA"
                     /locus_tag="OO572_003354"
     CDS             complement(6525..7460)
                     /gene="ldtA"
                     /locus_tag="OO572_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6662241.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7522..8601)
                     /gene="cobT"
                     /locus_tag="OO572_003355"
     CDS             complement(7522..8601)
                     /gene="cobT"
                     /locus_tag="OO572_003355"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6662242.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8613..9356)
                     /gene="cobS"
                     /locus_tag="OO572_003356"
     CDS             complement(8613..9356)
                     /gene="cobS"
                     /locus_tag="OO572_003356"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6662243.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(9353..>9497)
                     /locus_tag="OO572_003357"
     CDS             complement(9353..>9497)
                     /locus_tag="OO572_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6662244.1"
                     /translation="NEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGV
                     KIK"
BASE COUNT         2687 a         2400 c         2052 g         2358 t
ORIGIN      
        1 aattaccgct gatggtcagg gtattactct ggtgaatatt ggcgtgggac cgtccaatgc
       61 taaaaccatc tgcgatcatc tggcagtgct acgcccggat atctggttga tgattggtca
      121 ctgtggcgga ttacgtgaaa gccaggccat tggagattat gtccttgcac acgcttattt
      181 acgcgatgac cacgttcttg atgcggttct gccgcccgat atacctattc cgagcattgc
      241 tgaagtgcaa cgtgcgcttt atgacgctac caagttagtc agcggcaggc ctggtgagga
      301 agtcaaacag cggctgcgta caggtactgt ggtaaccaca gatgacagga actgggagtt
      361 acgttactca gcttctgcac ttcgttttaa cttaagccgg gccgtagcaa ttgatatgga
      421 aagtgcaacc attgccgcgc aaggatatcg tttccgcgtg ccatacggga cactactgtg
      481 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
      541 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      601 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      661 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      721 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      781 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      841 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      901 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      961 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
     1021 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
     1081 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
     1141 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
     1201 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
     1261 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
     1321 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
     1381 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
     1441 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1501 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1561 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1621 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1681 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1741 tctttatggc agatttcttt aatctcatat aattcttata aaaaatataa tatttaactc
     1801 gtcatattgc ttataaaaaa tccccgtccc cagagaaata atatttatta aaatggcagg
     1861 tcttcttttt ctgattaccg aagacaaagt ggcaattacg tatagtttcc cgataaagac
     1921 gcgacagcta cgtcccgcat aaggcatttt tctctttatc tttgtacggt acttcatgga
     1981 acagaatttt tgaccttgtg aatcgtgata tctgttgggg aggggcaatt tgctcactga
     2041 agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggc cactttttta
     2101 caaagtggcc gggtaaaaat aatttttatt ttttaactgt cctgagtctc atagagatgt
     2161 ctcaaaacta aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag
     2221 tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat
     2281 gcccacccac aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg
     2341 cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga
     2401 gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca
     2461 gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag catgattccc
     2521 tgcacataac cgactacaac ccggcaaccc cacatgctca acatagaaac ccacatggcg
     2581 taacgggcat cacgagcacc cttaaatcca gcgggtagca cccatgaggc ggaccaaata
     2641 ggcataaata aagcatttag ctaaatcaga atcacaacga cgtgtttaac ctgtggatcc
     2701 tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt
     2761 aatccaagag tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc
     2821 cctaccccca accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt
     2881 aagttgataa gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc
     2941 atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca
     3001 ctcgctggaa taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa
     3061 tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt
     3121 gcaactgcgc caatataacg agaaatggtt aaacccaacc cagccccgac caggagaaaa
     3181 ggccgtaaat caatatgccg ctaataataa tattaagaat attcaggcta ccgttaatca
     3241 gtagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
     3301 cagcggctgg ataactgagt acggtcagct ccagataagt caacgccagt gctttaactt
     3361 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaattagcg
     3421 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
     3481 ctcgtcgtcg atcccgctta ccgagactaa atgctacaac gacagtagta ccaagatcga
     3541 tagctgcaaa aaaagccata atgaccatat tgaagctgtc cgccaacccc acgccggcca
     3601 tcgcatcttt tcccagccaa ctgaccagaa aagtgctcag gacccccatc aacaggacac
     3661 aggcgttctc catgaagata ggaacagcaa gcggggttat ctcgcgctag aacaacactt
     3721 tgtagctctt gcgtttagag tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
     3781 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat gatgagggag
     3841 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
     3901 atagaaacgg caaacagttg ggaaatttaa aaatcgggtt tgacaaaata tttttcgccg
     3961 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
     4021 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
     4081 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
     4141 ggcgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccaaacat
     4201 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     4261 gccgcgtatc caggcggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     4321 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgtgcac
     4381 ttaatacaat atctgccggc aaacctttgc gggtaaatgc gtcatcagtg cgtgagcgcc
     4441 ccgtaattcc ctgtcggtaa gtaattaacg gccacttcgc tattgattcc agcgtcaatg
     4501 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacaaaacc
     4561 atgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     4621 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcga accctggatt agctcgagcc
     4681 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     4741 gagtatgcgt cgttgcaata gtgagaacac cagacgtatc gttggtaaac aggtctgcaa
     4801 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     4861 ccggttcagt catccccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaaatt
     4921 catcctccag ttcccgaata tgacggctaa cgcctgactg tgaggtaaaa agcatattcg
     4981 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     5041 aattcacggt aaactccggg cagttcagat atcccgttat tgttaaagtc taatgcccgg
     5101 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     5161 ttgccactgc cttttttcca tcactggaga actaatcact gacattaaca actctttcac
     5221 tgcctgtgcc tgtggtgaca agtttgctct ggcgggtaaa tttaaggaca aagagagact
     5281 cattgaagga gtggtaatgc gtgacatcca cccatttact gcgccacata acgaacgcgc
     5341 agccgattca ggtaatactg caacgcccat gccgctggca atcgctgcgg taagcgtggc
     5401 aatagactca atttcaccaa tgacttttgc cgtgagccgc cgtagggaaa aagcctcatc
     5461 aacacgaagt ctaacagcgc tgtaatcact ggggagaaaa aggttcattt gcgcaatagc
     5521 attaacatca acgttttgcc ccgggcaatc ttgagttcct accaggaaaa gatcttcttt
     5581 cagcaaagcc tgactggata ccccagccac aggggaatgc tcataaatca ccgccatatc
     5641 gagttggtga tttatcagtt tttcgttaag cactgcacca ctattttcat gaagatagat
     5701 aacgacctcc gggaattcag cgcgaaccgc ctgtaataag ggcatggtga tggatgacgc
     5761 agcggttcct ggcgcaaagc caatcgagac ttgccccgat aatgactgac caacgttatg
     5821 caccgccagt tgggcctgct cacactgacg taaaatggcc cgtgcatggg tatagagaat
     5881 ttttccggcg tctgttggcg taacgccccg ttttgtgcga atcaaaagct gttgatttaa
     5941 ctcaccttcc agtatggcaa cctgttggct gagcgctggt tgtgcgatat gcaatacttc
     6001 agcagcctgg gtcaggctac caatatctac aatttttacg aagtatttca ggcgtctgaa
     6061 gttcatgttg cctccggttt ttaagaatcg gcccaagtgc cgccattact tacaaccagt
     6121 atgcaagatg cctgccagtt ctattttggc gttgatgcgc aagctaacca actgtcaaat
     6181 aagagattat gatagattcg tcattggccc tttttatcag cagtcgcatt cacctgccct
     6241 ataaaaggag ggtatgcacc acgatggttc attacccaat aagattgaaa gctcaccact
     6301 ttgttgaaat tggcagcaaa caaacaaaaa aaatgcattt caccctttga catcaccatg
     6361 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     6421 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     6481 gcctgctttc gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt
     6541 gaaccagaac taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga
     6601 tttacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     6661 acttttcggt cagactcata ttctgcacga ttgcgcgaca gcggctcatg aacttccagc
     6721 cactttgaac catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacga
     6781 gtgcctacag gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag
     6841 ccctgactta cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     6901 ctgccaatat aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg
     6961 ggcaaactct cccctcgttt cgcatattcg cgccggatgt tcggcgttgg cgtccaggtt
     7021 ggtgcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     7081 tggccgatac caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga
     7141 cgcatttcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     7201 tgcggaatgg tgagttgtga gccaggcttc ggcaaaaaaa catcagcgcc cggattcgct
     7261 tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta
     7321 ttgtgatcag gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt
     7381 aatggataag ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga
     7441 atatttacac gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag
     7501 cttttatcag acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac
     7561 aatattactg gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat
     7621 ggacatcgcc agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg
     7681 ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg
     7741 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     7801 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc
     7861 tattccgacc aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt
     7921 tggttgattc aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt
     7981 cggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     8041 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac
     8101 accgtttttc gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     8161 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     8221 gataagcccg gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc
     8281 gcccgcttgt gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg
     8341 tatggctgtc acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc
     8401 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc
     8461 tgccagttgt atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgaataaccc
     8521 gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag
     8581 taaatcggca attatttgca tgtcacgttt ctttatagca gtgccagcag gaagaccagt
     8641 tcaccaagct ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg
     8701 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     8761 atacccagca ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt
     8821 cgcccatcaa ttttgccaat gaacacattg ccaagccctt cttcccgcgc ataacgatga
     8881 cgatacatca ataatgcggc agtcccgcgg ctgaccgcac atgctgccgc cagcgatgca
     8941 aggatcggtt cgccacgcag agccaactcg cttaacacca gaatctttgc cagtaccaca
     9001 aaaatcaatg ccagaccgcc gtgggtgcct aaacggctat cacgcatgat ttccaacatg
     9061 cgatcgcgac tacgtgcaga aaatacgcca tcacaggtat cagcgaggcc gtccagatgg
     9121 aattccccgg tcatcagcgc cagcacaagt acgctaaaca gtgccgccag tggtgcgcca
     9181 caccatgcct gcagcaccat gaagaccagc ccgctaatcg cgccaagcaa taatccaatc
     9241 aaaggaaaag taataatacc gcgagaataa tgttcgaaat ccagtccctg ggaccagcga
     9301 cgcggtacag gcagacgcgt aataaatgag agcatcgccc aaaataattt gctcatttga
     9361 tttttactcc aatacccgaa accaccagcc atacttcatt tgccgcagcg gccaactgct
     9421 gatttacccg cccggcaata tcacgaaaat gtcgtgccag acgactctcc ggcacaatcc
     9481 ccattcccac ttcgtta
//
LOCUS       DAJTYL010000131         9449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYL010000131 DAJTYL010000000
VERSION     DAJTYL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.131"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            376..1494
                     /locus_tag="OO572_003358"
     CDS             376..1494
                     /locus_tag="OO572_003358"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6662245.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1560..1808
                     /locus_tag="OO572_003359"
     CDS             1560..1808
                     /locus_tag="OO572_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6662246.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1873..2241
                     /locus_tag="OO572_003360"
     CDS             1873..2241
                     /locus_tag="OO572_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6662247.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2335..2988
                     /gene="nfsB"
                     /locus_tag="OO572_003361"
     CDS             2335..2988
                     /gene="nfsB"
                     /locus_tag="OO572_003361"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6662248.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3096..4343
                     /gene="ybdG"
                     /locus_tag="OO572_003362"
     CDS             3096..4343
                     /gene="ybdG"
                     /locus_tag="OO572_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6662249.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4411..5787)
                     /gene="pheP"
                     /locus_tag="OO572_003363"
     CDS             complement(4411..5787)
                     /gene="pheP"
                     /locus_tag="OO572_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6662250.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5888..9031)
                     /gene="cusA"
                     /locus_tag="OO572_003364"
     CDS             complement(5888..9031)
                     /gene="cusA"
                     /locus_tag="OO572_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6662251.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9043..>9449)
                     /gene="cusB"
                     /locus_tag="OO572_003365"
     CDS             complement(9043..>9449)
                     /gene="cusB"
                     /locus_tag="OO572_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6662252.1"
                     /translation="KTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQL
                     NTASEPMLLIPSQALIDTGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGE
                     KVVSGGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         2309 a         2622 c         2294 g         2224 t
ORIGIN      
        1 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa tcggaaagct
       61 tcaggcaccc gcagttatct tacccttcct caacaaaata gctgtttatt tatacagcaa
      121 atcctgtttc ggacttctca ctccccattt gatcaactga agctgaaccc gtttttttct
      181 gcatgcctca atcgtcaaaa ttcttcgcca ccagccagct ctgccataac ttgccacatt
      241 gaaaaagtag ccaaagtgct tcaggcacca tcagttcacc atcaccaaaa aaaagaaaca
      301 ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata gaaaacgtca
      361 acttaagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt taccctcggt
      421 attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca ggactcttca
      481 atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca cgatatcacc
      541 gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc tgccgggcag
      601 ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct ggaaatttgc
      661 ggcggtggca cgcccccgtt tcagaaatgg cagcgtcagg aggtatgcga caacgaacgc
      721 tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt ttttggtcag
      781 catgtccatg ttggctgtgc cagtggcgat gacgccattt atttgctgca cggcttgtca
      841 cggtttgtgc cgcactttat tgccctttcc gccgcgtcgc catatatgca gggaacggat
      901 acgcgttttg ccccctcacg accgaatatt ttttccgcct ttcctgataa tggcccgatg
      961 ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtctgag ttacaccacg
     1021 atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca ttttggcacg
     1081 gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa tatggcggga
     1141 ttaattcagg ccaccgccca ctggttactg acggaacgcc cgttcaaaca tcaggagaaa
     1201 gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatggtct ggaaggcgtc
     1261 attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagatacctt gcgattgctg
     1321 gaaaaaatcg ccccttcagc acataaaatt ggcgcatcga gcgcaattga agcactgcat
     1381 cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt cgccgatggc
     1441 ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccggtga ctaagccggg
     1501 aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt aacaatgtta
     1561 tgaaacaccc tttagaaacc ttgaccaccg cagcaggcat tttgctgatg gctttcctct
     1621 cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa ctggtgacca
     1681 tgttccatct gatggatctt agccagcttt acactttatt gttttgtctg tggtttttag
     1741 tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg ttctcgctgg
     1801 cggattaaac gtcgattatc gccgaacttc gcggcatact ttgcttattc tccttcgcct
     1861 aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct agctcttgag
     1921 ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt taaaattggc
     1981 ggcaagattt ttatgctgtc gtcggagcta cgcggcgtcc cctttatcaa tctgaagtcc
     2041 gatccacaaa aatccctgtt aaatcagcaa atatacccaa gcattaagcc agggtatcac
     2101 atgaataaaa agcactggat ttcggtgtat tccggcgagg aaatctccga agcgttactt
     2161 cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa acgcgatcaa
     2221 aaaagagtgc gtccaggcta aagcgggaat ctatagcgca tttttctcgc ttaccatttc
     2281 tcgttgaacc ttgtaatctg ctggcacgca aaattactta cacatggagt ctttatggat
     2341 atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag caaaaaactt
     2401 accccggaac aggccgagca gatcaaaacg ctactgcaat acagcccatc cagcaccaac
     2461 tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa
     2521 tccgctgccg gtaattatgt gttcaacaaa cgtaaaatac ttgatgcctc gcacgtcgtg
     2581 gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt tgaccaggaa
     2641 gatgccgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag
     2701 ttcttcgcct atatgcaccg taaagatctg catgatgatg cagagtggat ggcaaaacag
     2761 gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg cgctgggtct ggacgcggtg
     2821 cccatcgaag gttttgacgc cgcaatcctc gatgcagaat ttggtctgaa agagaaaggc
     2881 tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt taacgctacg
     2941 ctgccgaaat ctcgtctgcc gcaaaacacc accttaaccg aagtgtaatt ctctcttgcc
     3001 gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc tgtttcagcc
     3061 gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata tcccaggttg
     3121 aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt ttcggtatta
     3181 tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg cggaccttcg
     3241 aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag aataaactct
     3301 tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg gtattctggc
     3361 tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg tggatcatga
     3421 tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg gcgcagaaat
     3481 tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa ctgatcggcg
     3541 caatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca gcgattctaa
     3601 tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat ccgattcttg
     3661 gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc gactggctgg
     3721 aaatgccgaa atacggcgcg gatggcgcgg tgatcgatat tgggttaacc accgtcaaag
     3781 tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt tctgactcct
     3841 ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc agtatcagta
     3901 ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg aataaagcgc
     3961 atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg aatcgccagc
     4021 aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga acctttcgtg
     4081 cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg accttaatgg
     4141 tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg tttactaaca
     4201 ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac atatttgcga
     4261 ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat attcgctctc
     4321 tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga tgagagcgga
     4381 catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt taaatgccac
     4441 aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat catccatcgt
     4501 gcacatcagc agcaaaatca tgccgaggaa ggcgatgcaa agatagttgc cgaacggata
     4561 gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgccgcgc gaaaacgcag
     4621 atgcgccagg cagatcataa tccagttcaa cagcaacgtt gctaccacca gcgccatcag
     4681 cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg aagtgatcgc
     4741 tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag tcaaaaactt
     4801 cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt aaaccccgct
     4861 gttatacacc gacagcgatg ccactagaat gacgaagttc agcgcagaag ctaccacatt
     4921 gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca cttccaccca
     4981 cggatagagt gccagtaaaa ccaccagtga accgatataa aacagcagga tgcgatacac
     5041 cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag cggctgtaat
     5101 cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca aaatcagccc
     5161 attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac tggctttctc
     5221 gccgccgtaa ccagaaaaca gcagccacag gccaaagccg atcataccga tgatcgccag
     5281 cactttaatc agcgcaaacc agaactcggt ttcgccatat aagcgcacgt tcaccaggtt
     5341 aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa catccgggaa
     5401 ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca gcacgaacat
     5461 tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt tataggcaaa
     5521 gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct ggcgcataat
     5581 caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac ccgccatctg
     5641 aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca gcgcaatcag
     5701 ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg acgcagtatc
     5761 ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt tgttgagggg
     5821 caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt aaacgaggta
     5881 tcctgcttta attccgtacc cgatgtcggt gcagccacat cagcttatac gccgccggga
     5941 taataaacag cgacagcaaa ggtgcggtga tcatgccgcc aatcatcggt gcggcaatcc
     6001 ggctcatcac ctctgaacca gcccccgttc cccacagaat cggcagcaga ccggcgataa
     6061 tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac cgcgccgtga tacaacgcct
     6121 catccagctt ctgctcgctg aatgtttgtg gattattcaa cgacggctcg gcctctatgg
     6181 cgtgacgtaa atacatcagc atcaccacgc caaattcggc ggcgaccccg gcaagggcaa
     6241 taaagccggt acccgtcgcc acagaaagat gaaagcccat ccaccacagt aaccagatgc
     6301 cgcccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc acccgacgga
     6361 atgccagata caacagcacg aagatgatca tcagtgtcat cggcaccatg agtttcagct
     6421 tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg gtgcccggtt
     6481 ttaactgcac tttttcagct atcgcttttt gcaaatcgtg aaccaccgac accatgtcac
     6541 gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt ttcagcatcg
     6601 acggtccggt agagacttta acgtcggcca cgtctgccag ggtgatttgc tgcttcatcg
     6661 gcgtcaggat cggcaactgg cgcagtgcct gcggactatc gcgccagctt tgcggataac
     6721 gcagattaat tggataacgg gcaatccctt ccaccgtttc gccaaccatc gccccgccca
     6781 ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat accgtaacgt gcggcttttt
     6841 cacggttaat ctcaacgttg atatagcgcc caccttccag ccgctcagca agcgcagaag
     6901 ctacgcctgg caccgttcgc gccacttctt caatttgctc agccatcgcg tcgatatccg
     6961 ccagcacagt gccggaaact ttaatgccga tggggctttt aatgccggtt gagagcatat
     7021 cgatacggtt acgaattggc ggcacccaca gattcgccag ccccggcaga cgcacggtgt
     7081 tatccagttc ctcaatgatt ttgtccattg tcatgcctgg ccgccactga tcctgtggct
     7141 taagctggat ggtcgtttct accatctcca gcggagcaga atcggtggcg gtttccgctt
     7201 tcccggtttt gccaaatacc cgcgccactt caggtacgct cataattagc ttgtcggttt
     7261 tttgcagcat actcgccgcc tctgctgcgg aaatccccgg cagcgtcgat ggcatataca
     7321 acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc aactttattg agcggccaga
     7381 gaaccgtcag caccgaaagc gccgccacca gcagcgtggt tttcggccag tgcagtactt
     7441 tcagcaacag cggatgataa acacgaatca aaaagcgatt gagcggatta ctgctttccg
     7501 gcggaatttt gccacggatc cagtaaccca tcaggatagg gatcactacg atcgccagca
     7561 gcgccgcacc cgccatcgca tacgttttgg tgaacgccaa cgggccaaac agacgccctt
     7621 cctgcccttc cagggtgaag atcgggataa acgacaacgt gataatcagc agactgataa
     7681 acagcgccgg ccccacttca acagacgcat cggtgatcac ctgccagcgc gttttattat
     7741 ccagcgtggc gtcaggatgc tggtgctgcc actcttccag ctgtttatgc gcattctcga
     7801 tcatgacgat agcggcatcg accatcgccc cgacggcaat cgcaatgcca cccagcgaca
     7861 taatattggc attcagcccc tggaagggca tgacaataaa agcaatacac aaccccagcg
     7921 gcaacgaaat aatcgccacc agcgccgagc gcacatgcca gagaaacggc gcgcagacta
     7981 ccgccaccac aataaactct tccagcaact tgccgctgag gttgtcgatg gcccggtcaa
     8041 tgagctggct gcggtcgtat gttgtaacta tctccacgcc ttccggcaga ctacttttca
     8101 gcgtttccag tttgtccttc acggcggcga tcacttctcg ggcgtttttg ccggatcgca
     8161 ggatcaccac cccgcccgcc acttcgcctt cgccgtttag ttcggcaatg ccccggcgca
     8221 tcttcgggcc aatctggacc ttcgcaacat cgcgcagata aacgggcacg ccattttcac
     8281 tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg tagatagccg ctggcgcgca
     8341 ccatatattc tgcttccgcc agttcgattg acgaaccgcc cgcttcctgg tttgaagcat
     8401 ccagcgcgct ttttacttcg gcgagactga tgccatactg cgccaggcgc tgaggatcga
     8461 taaccacctg atactctttc accacaccgc ctaccgacgc cacttccgca acgtcaggaa
     8521 tggttttcag ctcatatttg agaaaccagt cctgcaatga gcgtaaatcg gcaagatcgt
     8581 gcttaccgct gcgatccacc agtgcatatt catagatcca gccaacaccc gtggcatctg
     8641 gtcccagctc ggcgctgact cccgcaggca gcttaccctg tacctggttg aggtactcca
     8701 gcacccgcga acgcgcccag tacggatcgg tgccatcttc gaaaatgaca tacacataag
     8761 agtcgccaaa ctgcgagaaa ccacgcacag tcttcgcgcc aggcaccgac aacatggtgg
     8821 tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctggccg ggatagctgg
     8881 ttttaataat cacctgcaca tcggagagat ccggcaacgc atccactggc gtattaatga
     8941 tggtccaggt gccccagatg ctcagaaaca acgcgcccat cagcaccaaa aaacggttcg
     9001 ccaccgagcg acgaataatc cattcaatca ttggttattc cctcaatgcg catgggtagc
     9061 actttcagag cgcatccgct ccagtgcgcc agaaatattg gcttcggaat caatcaggaa
     9121 caggccgccg gaaaccacct tttcaccttc cgccagacca gagcgtaatg cggtgacgcc
     9181 ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag cgcccgtcgg catcaacggt
     9241 aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga gcagcatcgg
     9301 ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc ggttttagcg cctcgtcggc
     9361 gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg gcatccacgc caggtagcag
     9421 cgtccatttg cggatggtga gtgttttat
//
LOCUS       DAJTYL010000132         9336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYL010000132 DAJTYL010000000
VERSION     DAJTYL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.132"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(56..829)
                     /gene="uxuR"
                     /locus_tag="OO572_003366"
     CDS             complement(56..829)
                     /gene="uxuR"
                     /locus_tag="OO572_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6662253.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1044..2504)
                     /locus_tag="OO572_003367"
     CDS             complement(1044..2504)
                     /locus_tag="OO572_003367"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6662254.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(2585..3769)
                     /gene="uxuA"
                     /locus_tag="OO572_003368"
     CDS             complement(2585..3769)
                     /gene="uxuA"
                     /locus_tag="OO572_003368"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6662255.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            4109..5452
                     /gene="gntP"
                     /locus_tag="OO572_003369"
     CDS             4109..5452
                     /gene="gntP"
                     /locus_tag="OO572_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6662256.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5703..6605)
                     /gene="fimH"
                     /locus_tag="OO572_003370"
     CDS             complement(5703..6605)
                     /gene="fimH"
                     /locus_tag="OO572_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6662257.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6625..7128)
                     /gene="fimG"
                     /locus_tag="OO572_003371"
     CDS             complement(6625..7128)
                     /gene="fimG"
                     /locus_tag="OO572_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6662258.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7141..7671)
                     /gene="fimF"
                     /locus_tag="OO572_003372"
     CDS             complement(7141..7671)
                     /gene="fimF"
                     /locus_tag="OO572_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6662259.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7681..>9336)
                     /gene="fimD"
                     /locus_tag="OO572_003373"
     CDS             complement(7681..>9336)
                     /gene="fimD"
                     /locus_tag="OO572_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6662260.1"
                     /translation="GPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQRE
                     GHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGK
                     NMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYF
                     NFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYL
                     SGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFS
                     HWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDG
                     NSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVV
                     LVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVAN
                     VVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLS
                     GMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2125 a         2486 c         2382 g         2343 t
ORIGIN      
        1 cttcttaatt taagaaagcg caaggaacgt ttacccttgc gcttattata ataagtcagg
       61 cgtcgacttt atccagcggc catgaatcaa agagatagcc gtcaaaataa atatcgtcaa
      121 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgctgccac attgccagct
      181 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
      241 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
      301 tttcccgcca ctgccaggac tgacggaata actccaccag catgctgtta tgcgttgctt
      361 cagcaatggc gagatggaac tgcatgtcac cgctttcgct gctacccggc gcgctggaag
      421 ccagttcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
      481 cctgcagtgc ggcaaactcg gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
      541 caaaaggacc cgcatcattg cagacgttgg catccggact gtctgtgttg tggctgcctg
      601 agctatcaag cacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
      661 gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct gcaatttcac
      721 gctccggcgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga tcgtggatca
      781 tcgccccgac ttcctggtaa ggtctttgcg cagaggtgac agatttcatg gtgttgtgtc
      841 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
      901 aacgggcaat tttacggccc ccgttaatct caaactatac cattagcact ggaagttggc
      961 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1021 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1081 cacagctgct gatatgccga cgtcaccgcg ccaacaaagt cggcattctg cggcagatca
     1141 tcggcaaaaa taccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     1201 gcgttaatct tctggaactc ggccagcatc gggtcgacca cgtcaatggc attaccctgc
     1261 tcatccacgc cctgggtgta acccatccag ccagccacgc ccagcgccag gtgacgccag
     1321 ctgccgccgt tttgcaggtg cagacgcacc gggtccagta gacgctgcgg taacttctgg
     1381 ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     1441 atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag cgttggcgct
     1501 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccgggttagt catggtgtcg
     1561 gcaatggttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     1621 agcatacgca gcttcatcat ttcgaacggc acaacgtctg caacgaactg tgtgcccact
     1681 ttatcccagt ccgggcgacc attaacgaag ttatcttcaa tcacccactg acggaacggt
     1741 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     1801 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     1861 tcaatccatg ccgctagctg cgggtcacgc gcctgagcca gccccagtac cgcgaccttc
     1921 gccacatggc cgttttcacg cacgttatcg caggacatca ccgtaaatgc tttcagccct
     1981 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2041 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagttgacc gcttgccgca
     2101 tcagcgcagt agcctttttc cgtgaccgtt agagagacaa tcgccgtttg cggacgcgcc
     2161 atcgcgttga gaataccttc gcagccgtcg atttccggat gcagcgcttc tttcatcgaa
     2221 ccgataattt tcagctcggt gctttctgcg cctttttccg ctacggtgta cagcagttgc
     2281 tgttttttca ggttttcgat cagcatgcgg tcgttgcctg gcatcaggtt aacttcgcag
     2341 atgccccagt cgctgtcggt gctttccagc agatgatggg tatacagcgc ctggtgcgcg
     2401 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     2461 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     2521 cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     2581 ggtattaacg gataaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcggcca
     2641 ggcctttcag acgaccaatt gcggagtaac cagggttggt tttcttcttc aggtcgtcca
     2701 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     2761 gacggtgttc ttcttcaaca atcgctttca ccactttgta catatcaacg tcaccgttca
     2821 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     2881 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     2941 cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3001 tccactgcat atcttcaatg gtggaaacaa tgcgcggcaa gccgaggatc gggcgcggcg
     3061 gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc agcaactgga ataatcgctt
     3121 tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc gatatctttg tacagctcca
     3181 ggtgcttacg gaactggtcg agggtatacc cttcttccgc gcccggcaga ccggcaatga
     3241 tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt tcagctgcct
     3301 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgtttcagg atatgcattt
     3361 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     3421 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     3481 tgccacactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgctcatagt
     3541 tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct ttctacgaca gaccacacca
     3601 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     3661 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     3721 ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     3781 tttcacatag tgggttccac agcaaagaga cgcggaacct tggagttgtt taggtatccg
     3841 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     3901 cttttggtaa gacatgtcaa ccagaattct gaaattggtt aaccgcgtcg caagaatttc
     3961 actctcatgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4021 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4081 acggttcaac caagtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     4141 cggcattggc ctgatgctga tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     4201 gttggtggcg gcgctgtccg tcgggatgct ggcaggcatg gatttgatgt cgctgctgca
     4261 caccatgaaa gcgggcttcg gcaacacgct gggagaactg gctatcatcg tggtgttcgg
     4321 tgcggtcatc ggtaaattga tggtcgactc cggcgcggct caccagatag cacatacgct
     4381 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggtg attatcatcg gccttatttt
     4441 cggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgttat
     4501 tgccgccgaa gctaaaattc cgttcctgaa actggcgatc ccggcagtag cagctgccac
     4561 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     4621 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtaatctg
     4681 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     4741 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     4801 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     4861 agatacccct gcctgggaag tggttaactt tattggttcc tcgccgattg cgatgtttat
     4921 tgcgatggtg gttgcattcg tactctttgg caccgcgcgt ggtcatgaca tgcagtgggt
     4981 gatgaacgct tttgaaagcg cggtgaagag tattgcaatg gtgattctga tcatcggtgc
     5041 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggcgacacca tcggcatgtt
     5101 gatgtcccac ggcaatatct cgccctacat tatggcatgg ctgatcactg tgctaattcg
     5161 tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc
     5221 aatccttgat ccagcaaccg gtcagctggt tggcgtgaat ccggcgctgc tggtactggc
     5281 gacggctgcg ggttccaaca ccctcaccca cattaatgat gcatctttct ggttgttcaa
     5341 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt
     5401 caactccgtg gttgggctga ttattgtgct gattattagc atggtagcgt aaaagaacac
     5461 cagagcctgc caatggcagg ctcagactga tgactaaagt ctaaaaatgt gcccggacag
     5521 tcccctcgcc cctccgggga gagggttagg gtgaggggaa cagaccagta aagtgcgctt
     5581 tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag
     5641 gttttgtagg tctgatagcg tagcgcctca ggtaccagca ttagcaatgc gctgtaattt
     5701 ttttattgat aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc
     5761 cctccggtac gtgcgtaatt tgccgttaat cccagactta ccgccgaagt tcctactgct
     5821 cctaacgata ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg
     5881 acgccctgcg ctggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg
     5941 gttgtgccgg agaggtaata ccccaggttt tggcttttcg cacaataaac ggtaagagga
     6001 attggcactg aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatca
     6061 caaccgccag tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa
     6121 tcatcgctgt tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct
     6181 ttaatagcca ccccgcccgc actgctcaca ggcgtcaaat aaagcgccac cggccacggc
     6241 ttatccgttc tcgaattata aacaacccgc ggcgtttcgc tggtggtcgg aaatggatag
     6301 ctactgccac tatattttac ggtcccggaa aaattagata acacgccgcc gtaagccgag
     6361 cctcgttgca gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt
     6421 tgcgtcgaaa gatctacgac caggttttgc cccacattca cgacaggcgc aaggtttaca
     6481 taaacattag cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg
     6541 aatgaccagg catttaccga ccagcccatc agcagtacag caaacagggt aatagctcgt
     6601 ttcattacaa ttatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg
     6661 aatggttccc tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg
     6721 cgctgattgt gaggaatcat ccacctgaac tgttttggtt gcgccagtat tcaatgtgtt
     6781 gccactgtca tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata
     6841 tccggtgctg tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca
     6901 attagtcaac tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact
     6961 gaaagaataa agatcgccga gatctaccgt ggcattggtg gtggaaaccg tacacggttt
     7021 ggcgacgacc ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc
     7081 gagcgccaac attgccgcca atacataccc acgtttgcac catttcatga gcatctccag
     7141 ttactgatat tcaagagtga aggtagccgt agcattgata tgccccgcag tgacaggcac
     7201 ctgtgtcgcc attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt
     7261 cgtccacgaa atcgcagacg atggagcatt aaggggtatt tgattttgct gctcattcag
     7321 aagctgtatt cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc
     7381 attgtggcta tctgcaacgc cggtaaaccc aacttttacg gcagaaacgg cattaccaca
     7441 gggtgacagc aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg
     7501 cttcgccgcg ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca
     7561 gccgttatct ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aaacgcgact
     7621 taccgccagc cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc
     7681 ttaacgacat tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg
     7741 ataattggcg acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc
     7801 taaaggcatt ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct
     7861 actctctgat gtcaccatcg ccccaaacgg cagcggctta ttattgtgag ttagcgtcat
     7921 gagcagtttt atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg
     7981 aacaacgtta gcgaccgcgt tatctaaatc gacgttatca gccaaggtat tggtatccag
     8041 cgccactcta ttttcccgat attcagtggc ataaggcagc acggcataac cacgccagtc
     8101 ggtacgcacc cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag
     8161 caccaccgta tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccaatacccc
     8221 accgctgact ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatattggc
     8281 attgccgtaa ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc
     8341 gcctcccccg gcatagccgg tttgcacgct ataactgagg ttgttgtctt ccagcaacgt
     8401 accgtataca ccagccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtagct
     8461 ggcactggca tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat
     8521 attgacgtta agcgctaaca tctgatcccg tcctttttgc caggcgtttt tcgtcaggct
     8581 atagctgagc gtccagttga tatcttcgaa cgcagtattt aatccagcct ggaattgctc
     8641 atcgacatta ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatgt
     8701 gcgcccgagt tgctgagtaa cggtgagctg taatttcccg cgtttgttat aagcgaggtt
     8761 gtaatagtcg gtgaatttcg gcttaacctg aataactcca tcctgtgttt cgatgttgta
     8821 gccattcatt cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg
     8881 gtaacccact aactgaatat tcgtgcctga ttcattgagc gatttgttat agagaaaacg
     8941 caccgattgt ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc
     9001 cacagacaga gcgcccagtg cctccatgtt tttcccgata ccgaaattaa aagcacgata
     9061 acgatccgcc agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa
     9121 tgtactctgg aaaaagcggg gtttttcctg ctgcgcattt ccactacggt attctcctgc
     9181 cgtaatggaa taacgagtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac
     9241 ggtaaaaatc tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt caccactatt
     9301 acctgcggca tagatatcgt tgatggtaaa aggccc
//
LOCUS       DAJTYL010000133         9296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYL010000133 DAJTYL010000000
VERSION     DAJTYL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.133"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(292..1269)
                     /gene="epmA"
                     /locus_tag="OO572_003374"
     CDS             complement(292..1269)
                     /gene="epmA"
                     /locus_tag="OO572_003374"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6662261.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1594..3402
                     /gene="frdA"
                     /locus_tag="OO572_003375"
     CDS             1594..3402
                     /gene="frdA"
                     /locus_tag="OO572_003375"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6662262.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3395..4129
                     /gene="frdB"
                     /locus_tag="OO572_003376"
     CDS             3395..4129
                     /gene="frdB"
                     /locus_tag="OO572_003376"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6662263.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4140..4535
                     /gene="frdC"
                     /locus_tag="OO572_003377"
     CDS             4140..4535
                     /gene="frdC"
                     /locus_tag="OO572_003377"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6662264.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4546..4905
                     /gene="frdD"
                     /locus_tag="OO572_003378"
     CDS             4546..4905
                     /gene="frdD"
                     /locus_tag="OO572_003378"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6662265.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4968..6100
                     /locus_tag="OO572_003379"
                     /pseudo
     CDS             4968..6100
                     /locus_tag="OO572_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6189..6722
                     /gene="blc"
                     /locus_tag="OO572_003380"
     CDS             6189..6722
                     /gene="blc"
                     /locus_tag="OO572_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6662266.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6719..7036)
                     /gene="sugE"
                     /locus_tag="OO572_003381"
     CDS             complement(6719..7036)
                     /gene="sugE"
                     /locus_tag="OO572_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6662267.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7212..7358)
                     /gene="ecnB"
                     /locus_tag="OO572_003382"
     CDS             complement(7212..7358)
                     /gene="ecnB"
                     /locus_tag="OO572_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6662268.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7469..7594)
                     /gene="ecnA"
                     /locus_tag="OO572_003383"
     CDS             complement(7469..7594)
                     /gene="ecnA"
                     /locus_tag="OO572_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6662269.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7646..8212)
                     /gene="efp"
                     /locus_tag="OO572_003384"
     CDS             complement(7646..8212)
                     /gene="efp"
                     /locus_tag="OO572_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6662270.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8254..9282
                     /gene="epmB"
                     /locus_tag="OO572_003385"
     CDS             8254..9282
                     /gene="epmB"
                     /locus_tag="OO572_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6662271.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2329 a         2408 c         2395 g         2164 t
ORIGIN      
        1 caaatccaaa tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta
       61 tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt ataacacgtg cccactgaac
      121 tgcactgcgc aacattgagc aggcagcgta aaaaccaata cagaattgat acttggatga
      181 tatcaaatat ccagtcttaa attgtggcct ttaacggaaa aatgaaaaca tatgctgcag
      241 aatggcgctt atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg
      301 gtcaacgcta aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag
      361 acgatcaaca cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat
      421 cagattctgg tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg
      481 ttcaaagcgt tgttgctgct cacgggcatc cgttaattca tggaaaccat tcgccagctc
      541 aataccttta taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg
      601 cgccagtgat gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttaccaat
      661 atttggctct acgccaaagg taaacagcaa ttgtagcaac gtgtcgcggt cttcttcggt
      721 atcagcaaca ttgctcaaat ccagtttcgc tgcgacttcc cgcagttgcg ttttgtcggc
      781 agagagcggg tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc
      841 tgccgggcag tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat
      901 atcatagtgc ggtcgatacc actccagcat agtgaactca gggttgtgat aacgccccat
      961 ctcttcatta cggaagctgc ggcacagctg gaataccggc ccacaaccag caaccagcag
     1021 gcgtttcata tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc
     1081 ggggccaacg aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct
     1141 catacaaggc gtctccacct ccagcactcc acgatcggca aagaaacgac ggatctccgc
     1201 cataatcgcc gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt
     1261 ttcgctcata gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac
     1321 ggcgagacaa attttacgca ggaatcaaac agcggtgggc agtgactaaa aaaagcacga
     1381 tctgatggtt tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact
     1441 aaaaaaatcg atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata
     1501 aaggagcagt ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg
     1561 ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc
     1621 attgtaggcg ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca
     1681 aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca
     1741 gaagggggct ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat
     1801 acagtagcgg gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac
     1861 tgcccaaccg aaatgaccca actggaactg tggggatgcc catggagccg tcgcccggat
     1921 ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg gttcgccgcc
     1981 gataagaccg gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag
     2041 atccagcgtt ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc
     2101 ggcctggtag caataaacat gatggaaggc acgttggtgc agatccgtgc taacgcggtc
     2161 gttatggcta ccggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt
     2221 accggtgacg gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc
     2281 gttcagtatc acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgt
     2341 ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg
     2401 ggcccggaaa ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac
     2461 aaagtttctc aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt
     2521 ggtgatgtgg tttatctcga cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg
     2581 ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt
     2641 ccggtacgtc cgactgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa
     2701 acccgcatta aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca
     2761 aaccgtctgg gctccaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa
     2821 caagcgacag agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag
     2881 gcagctggcg ttgaacaacg tctgaaagat ctagttaacc aggatggcgg cgaaaactgg
     2941 gcgaagatcc gcgacgaaat gggcctggca atggaagaag gttgcggtat ctaccgtacg
     3001 ccggaactga tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc
     3061 gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg
     3121 agccacggtc tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc
     3181 cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caactttctc
     3241 aaacacaccc tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg
     3301 aagattacta cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat
     3361 aaggcggaag cagccaataa taaggagaag gcgaatggct gagatgaaaa acctgaaaat
     3421 tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt
     3481 gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc
     3541 accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat
     3601 ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat
     3661 gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca
     3721 cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga
     3781 tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg
     3841 catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat
     3901 cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa
     3961 gaaggagcgt atggcgcagt tgaacagcca gaacggtgta tggagctgta ctttcgtggg
     4021 ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa
     4081 agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca
     4141 tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc
     4201 cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca
     4261 ttgaactgat tttcgcgctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg
     4321 tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg acggcagctc
     4381 tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag
     4441 acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca
     4501 ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat
     4561 ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc
     4621 gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt
     4681 ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc
     4741 gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac
     4801 gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat gggttttcta cggtctggct
     4861 gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat cgccaatgta
     4921 aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga
     4981 cgctctgcgc cttattaatt accgcctctt actccacatt tgctgcccct caacaaatca
     5041 acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg
     5101 cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg
     5161 ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat
     5221 ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatcccg
     5281 caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc
     5341 ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa
     5401 gcgacttgct gcgcttctat caaaactggc agcctgcatg ggctccagga acacaacgtc
     5461 tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga
     5521 gttttgagca ggcgatgcaa actcgtgtct tccagccact caaactcaac catacgtgga
     5581 ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag
     5641 tgcatgtttc gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg accattgaag
     5701 atatggcccg ctgggtgcaa agcaatttac cccccttgat atcaatgaga aaacgcttca
     5761 acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
     5821 gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg gcagtgacaa
     5881 taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc ctgcagtacg
     5941 cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg tcgcgtttat
     6001 tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca atccagcgag
     6061 agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
     6121 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
     6181 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
     6241 cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc gacgccaaac
     6301 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
     6361 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
     6421 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
     6481 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
     6541 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
     6601 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
     6661 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
     6721 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccagac
     6781 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
     6841 caatacccgt ccacacggca taagccgtcc ctaccggtaa cgatttcatc gcccaggcaa
     6901 gtagcgccat actgactatc atcgccgtca cggtaataac actcggcgtc aaacggctaa
     6961 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
     7021 agataatcca ggacatatca ggctccggaa atttggggcc gtccccggtg aaaagaggcg
     7081 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
     7141 tttcaactat tacctttatg acgatcgcag aaaaaacaaa cggcacgaca ctgctgtcgt
     7201 accgttattg cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca
     7261 gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc
     7321 agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg
     7381 ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat
     7441 ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttaccga
     7501 gatgtttgat gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc agcgtgctgg
     7561 caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca
     7621 gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg
     7681 tatccacttt gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag
     7741 tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg
     7801 tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc
     7861 acagagttac gatacactct gcctgatcca gcagccattt agcattgtcg ccaattgctt
     7921 ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt
     7981 cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag
     8041 atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa
     8101 atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt
     8161 ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct
     8221 ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc
     8281 ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt
     8341 ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag
     8401 ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac
     8461 gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc
     8521 tccaccgacc cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac
     8581 aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt
     8641 cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac tgcacttgag
     8701 tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg
     8761 gcgaaagagc acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa
     8821 cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg
     8881 gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc
     8941 aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc
     9001 ctgcttaacc agagcgttct gttacgtggt gtgaacgata acgcacaaac gttggcaaac
     9061 ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctagataaa
     9121 gtacagggcg cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag
     9181 ttgctgacat tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa
     9241 cccagcaaaa cgccgctgga tctccagcta cgccagcagt aactgattaa acaggg
//
LOCUS       DAJTYL010000134         9236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYL010000134 DAJTYL010000000
VERSION     DAJTYL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.134"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO572_003386"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO572_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6662272.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO572_003387"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OO572_003387"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6662273.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO572_003388"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OO572_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6662274.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OO572_003389"
     CDS             complement(2404..2607)
                     /locus_tag="OO572_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662275.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OO572_003390"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OO572_003390"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6662276.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OO572_003391"
     CDS             complement(3803..4015)
                     /locus_tag="OO572_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6662277.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OO572_003392"
     CDS             4275..4583
                     /locus_tag="OO572_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6662278.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO572_003393"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OO572_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6662279.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO572_003394"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OO572_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6662280.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OO572_003395"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OO572_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6662281.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OO572_003396"
     CDS             complement(8479..8982)
                     /locus_tag="OO572_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6662282.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9236)
                     /locus_tag="OO572_003397"
     CDS             complement(9070..>9236)
                     /locus_tag="OO572_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6662283.1"
                     /translation="DSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2291 a         2363 c         2192 g         2390 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgct gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcac
//
LOCUS       DAJTYL010000135         9127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYL010000135 DAJTYL010000000
VERSION     DAJTYL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.135"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..102
                     /locus_tag="OO572_003398"
     CDS             <1..102
                     /locus_tag="OO572_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR6662284.1"
                     /translation="DGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            161..817
                     /gene="pdxH"
                     /locus_tag="OO572_003399"
     CDS             161..817
                     /gene="pdxH"
                     /locus_tag="OO572_003399"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6662285.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            946..2220
                     /gene="tyrS"
                     /locus_tag="OO572_003400"
     CDS             946..2220
                     /gene="tyrS"
                     /locus_tag="OO572_003400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6662286.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2279..3142
                     /gene="pdxY"
                     /locus_tag="OO572_003401"
     CDS             2279..3142
                     /gene="pdxY"
                     /locus_tag="OO572_003401"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6662287.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3186..3791)
                     /gene="gstA"
                     /locus_tag="OO572_003402"
     CDS             complement(3186..3791)
                     /gene="gstA"
                     /locus_tag="OO572_003402"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6662288.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3897..5399)
                     /gene="dtpA"
                     /locus_tag="OO572_003403"
     CDS             complement(3897..5399)
                     /gene="dtpA"
                     /locus_tag="OO572_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6662289.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6010..6645)
                     /gene="nth"
                     /locus_tag="OO572_003404"
     CDS             complement(6010..6645)
                     /gene="nth"
                     /locus_tag="OO572_003404"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6662290.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6645..7340)
                     /gene="rsxE"
                     /locus_tag="OO572_003405"
     CDS             complement(6645..7340)
                     /gene="rsxE"
                     /locus_tag="OO572_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6662291.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7344..7964)
                     /gene="rsxG"
                     /locus_tag="OO572_003406"
     CDS             complement(7344..7964)
                     /gene="rsxG"
                     /locus_tag="OO572_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6662292.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7968..9026)
                     /gene="rsxD"
                     /locus_tag="OO572_003407"
     CDS             complement(7968..9026)
                     /gene="rsxD"
                     /locus_tag="OO572_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6662293.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2339 a         2284 c         2320 g         2184 t
ORIGIN      
        1 gacgggatct atgttttctg gtcgaaaggt gaggaagcga ctgtctataa acgcgaccgc
       61 atcgtcttga ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc
      121 gcacaatagc gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt
      181 gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gccgcgatct
      241 tcccgccgat ccattaaccc tttttgaacg ttggctctct caggcttgtg aagccaaact
      301 ggcggaccct accgcgatgg tggtcgctac cgtggatgaa catggtcagc cttatcagcg
      361 catcgtttta ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag
      421 ccgtaaagca catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac
      481 ccttgaacgc caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat
      541 gaaatatttt catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc
      601 cagtcgcatt tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt
      661 tcaacagggc gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca
      721 gattgagttc tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga
      781 aaatgatgcg tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta
      841 atcgctggta ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag
      901 tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac
      961 ttgattaaac aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta
     1021 gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac
     1081 agcttgcatt tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc
     1141 cacaagccgg ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa
     1201 gctgccgagc gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt
     1261 aagcaggttg ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac
     1321 aactatgact ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac
     1381 ttctccgtta accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat
     1441 caggggattt cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt
     1501 ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc
     1561 acttctggta tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt
     1621 ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg
     1681 ttggatccga agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat
     1741 gccgacgttt accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc
     1801 ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag
     1861 caggtgactc gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa
     1921 tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag
     1981 gacggcgtac cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat
     2041 tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc
     2101 attaacggtg aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt
     2161 ggtcgtttta ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa
     2221 tgcattaagt ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat
     2281 gatgaaaaat attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc
     2341 ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt
     2401 ttctaatcac acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac
     2461 cgaaattgtg caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag
     2521 tggctatctg ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa
     2581 agcagcgaat ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg
     2641 ctgtatcgtt gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga
     2701 tatcattgcg ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt
     2761 cgaagaagcg gttctggcag cgcgcgaact cattgagcaa gggccacaaa ttgtgttggt
     2821 taaacacctg gcgcgagctg gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc
     2881 cgatgaagtc tggcatatca gccgtccact ggtggatttt ggtatgcgcc agccggtagg
     2941 tgttggtgat gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca
     3001 ggaggccctg gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat
     3061 gcaggaatat gagctgcaag tggtggctgc tcaggatcgt attgccaaac cagaacatta
     3121 cttcagcgca acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat
     3181 ggctattact ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata
     3241 cgttgcataa atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc
     3301 cagcgcagaa ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag
     3361 tgctcatcct tcagtgcctc gttcacatat tgcagcttct tatccagctg cgcgcgaact
     3421 gtcggtttgt actcttccgg tgtatctggg cgaaacagag gtgtgaaacc tttatgcagc
     3481 tcggtggcga tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt
     3541 gccagcaact ggcggtcggg gacgctgtcg gcaagatact gcataatcgc aacgccttcc
     3601 gtcagcaaag taccatcgtc cagcagcaat gcaggcacct gccccttagg gttaacggaa
     3661 aagtaatcgt caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa
     3721 tcctttccgc tctcacgcag ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag
     3781 aacaatttca tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa
     3841 caaaaaaaaa gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac
     3901 gctacagtag ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagttta
     3961 ggcgcggtca gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat
     4021 acgcgaccat agacttccag tgacatcagc gggtcggtaa cgttatccgg cacagccatc
     4081 atacccgcaa cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc
     4141 ataatgaagc ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca
     4201 gagatcatca gttccccgat gctctgcagg ccatagcttg cgaccagcca gctgacagac
     4261 acgatacctg cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa
     4321 cacatcacca tgccgattgc aaacttggtt ggcatcggca gggtatcacc catcttgtta
     4381 tagatagcgg ccagaatcgg actaccgatg atgatccaga acgggttcag tgcctgatac
     4441 tgctcaggtt ctacggccag acccagaatg gagtgctcaa cgttacgaat cgcaaagaag
     4501 ttcagtgacg ttggcatctg gctgtacagc acgaagaaga tgattgcttc gagcatcagg
     4561 atgaaggcaa cgatcatttt acgacgcgca gcacctttca tcgcgaaagc ttctttaccg
     4621 aagataacca caataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg
     4681 tgcagtagcc aggtggcaac agcgatcagc gccacaacac caataatggt cagcaacagg
     4741 ttacggtagt tgactggttc aaagtctggt tttgaaccgt actgtttaac ccagcgttgg
     4801 cagaaggcga agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca
     4861 ctccagccgt atttcgcggc cagccacggg gtggctatca tagagaagaa agagccgatg
     4921 ttgacggaca tgtagtacat ggtgaatgca ccgtccagac gtgggtcgtt tttctcatag
     4981 catgtagaaa gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc
     5041 gccataccca tataaacaat accggcgtcg tgaccagacc aggcaaccag agcataacca
     5101 atcgccagca caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct
     5161 aaccagccgc cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg
     5221 attgaatccg cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt
     5281 aggccgtaat aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc
     5341 ggttgtttga aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact
     5401 tttacctctt tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa
     5461 acatacgcgc aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac
     5521 tttcagacaa gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat
     5581 tccgggaaat gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact
     5641 gcattgagag ggggatgatg gcttggcggc gatatgttct gctatttagc gtttttccta
     5701 gtgagatagt ccagtttctg aaaaatagcc agtgtaatgt tttgcaggtc aatatttgtt
     5761 gcgtgtgtaa atttaaatca ttttaacctg attcttgcaa tgtttttaca ttgccacagc
     5821 atcgtgatct tgatcacgaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc
     5881 atttttggtt atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac
     5941 gttaaaaaga tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac
     6001 cccttttctt cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga
     6061 gccacagcgg ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg
     6121 gcagtcgact ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac
     6181 gtttttcccc ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc
     6241 aatagtcggc cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc
     6301 gggcagggct tcaagcgcag cacgatcttc cggaacctcg ccattatgtt gttccagtaa
     6361 tatacggcag gttttgatga tattttctgc tttgctgtta taaaggccaa tcgttttgat
     6421 ataggttttc accccttcaa cgcctagttc aagcatcgct gcaggcgtat tcgccaccgg
     6481 gtagagtttc gccgtcgcct tattaacact gatatcggtc gcctgagcgg aaagcagtac
     6541 ggcaatcagc aattcaaaag gcgaactgaa attaagctcg gtggtgggat gaggattgtt
     6601 ctcacgcagg cgagtgagga tctccagacg ttttgctttg ttcatcagac attccctgtt
     6661 tcaccgtttg gcaatgcacg ttcggcagct gcttcagcac ggcgcttttt cattctttca
     6721 tcaatcaggt attttcctgc cagcatcagt cccaagccaa taaatgcacc tggtggcagc
     6781 atcgccagca ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag
     6841 ctacctaaca gcgcatctgc accgtcaaac aacgtgccat tgccgataat ttcgcgtagt
     6901 gaacccagca cgaacatggc gcaggttgcg cccgtaccaa ttgaaaagcc gtccagtgcc
     6961 gaaagcgccg gacctttttt ggcggcgaag gcttcagcgc ggcccacaac gatacagtta
     7021 gtgacaatca gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg
     7081 atcagcatct gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg
     7141 atctcggctg gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc
     7201 agcgtagtcg caagtcctaa acccagagcg ttagtggcag tggatgtgac cgccaatagc
     7261 ggacaaaggc cgagcagttg gaccagtgca gagttgtttt tccacaaccc ctgaacaata
     7321 acgtctttaa tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgtgcc
     7381 ggtaacgtct gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta
     7441 atcgtcgcgc cggtgaactg gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca
     7501 tcatccgcac cactgatttt ttttcccgca aaatgggtga gccagtcaga aaggcgcaat
     7561 tcgattttat cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc
     7621 gtgccattaa aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg
     7681 gttgcttcca gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc
     7741 tcacctttac ctaactctgg cgcagttacc agatagcaac tctgtgcgag cgcattgtta
     7801 tagcgttcgg ctggcagcac ctgatcaaat aacgtctttt gttgcagact ggcctgttca
     7861 gcaattgtcg ttttggtcat ctggttgatg gccgcagtta accctgtaga acccgctgca
     7921 aatagtgcca gtgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga
     7981 tggccgtaga cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc
     8041 aggacggcaa aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat
     8101 aagcccgcca gcgcgccgaa catcagccga ccacgattgg tcgtagaagc agtaactggg
     8161 tcagtcaaaa taaagaatgc gccgagcata gtcgcaccag acagcagatg aatttgcggt
     8221 gctgccagtg tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact
     8281 aagaagctga ggggaatatg ccagcgaatc gctttctgcc atagcaacca cacgccacca
     8341 gccagccagg cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc
     8401 ggatattgca taatctgttc aaccgaatga cctgcgcgga cagaggtttt gaatgtatcc
     8461 agcggtgtcg cctgactaat gccatcaata cctaagcgta gcgtattcat atcaccacca
     8521 ctggcggtat gaccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc
     8581 gcaatttcat gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc
     8641 acataaccaa tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta
     8701 gcgatgatca ccgcaaacac cgtacccagt acgaccatcc accatggcgc taggggggga
     8761 atacttaccg ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgttgcggct
     8821 accgactgct tgcgtagttt gagtacgaga gcttcggcta acagagcact gaccgacgcc
     8881 aacaggatct gaacgagagt accccaacca aaaaaccgca gttgcgctgc gattcctggc
     8941 acggctgcga gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa
     9001 ggggagctag ctattctgaa taccatttag tcctcgttta caaccttttg ctgggcggct
     9061 tttttggcct gaacgcgggc aatagccgcg gcaactgcgg ctttgcgcgg atcgacctgt
     9121 tcttctg
//
LOCUS       DAJTYL010000136         9068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYL010000136 DAJTYL010000000
VERSION     DAJTYL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.136"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO572_003408"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO572_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6662294.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO572_003409"
     CDS             1828..3147
                     /locus_tag="OO572_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6662295.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO572_003410"
     CDS             3445..3486
                     /locus_tag="OO572_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662296.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO572_003411"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO572_003411"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6662297.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO572_003412"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO572_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO572_003413"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO572_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO572_003414"
     CDS             5815..6711
                     /locus_tag="OO572_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6662298.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO572_003415"
     CDS             6708..7682
                     /locus_tag="OO572_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6662299.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO572_003416"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO572_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>9068)
                     /gene="osmC"
                     /locus_tag="OO572_003417"
     CDS             complement(8657..>9068)
                     /gene="osmC"
                     /locus_tag="OO572_003417"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6662300.1"
                     /translation="QAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEKGTNPEEL
                     IGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKSEVAVPGI
                     DASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2197 a         2226 c         2285 g         2360 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctga
//
LOCUS       DAJTYL010000137         9064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYL010000137 DAJTYL010000000
VERSION     DAJTYL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.137"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..822
                     /locus_tag="OO572_003418"
     CDS             <1..822
                     /locus_tag="OO572_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6662301.1"
                     /translation="STVIGQAIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWY
                     RNRAQFVLTDEERTYLSASVKPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYI
                     AWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAI
                     VCGLMMSALGTLLVVQSSSPAQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIA
                     GIQNFGSFVFASFAPIVTGWVVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1074..1991
                     /gene="oxyR"
                     /locus_tag="OO572_003419"
     CDS             1074..1991
                     /gene="oxyR"
                     /locus_tag="OO572_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6662302.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1974..3374)
                     /gene="sthA"
                     /locus_tag="OO572_003420"
     CDS             complement(1974..3374)
                     /gene="sthA"
                     /locus_tag="OO572_003420"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6662303.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3695..4345
                     /gene="fabR"
                     /locus_tag="OO572_003421"
     CDS             3695..4345
                     /gene="fabR"
                     /locus_tag="OO572_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6662304.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4345..4704
                     /locus_tag="OO572_003422"
     CDS             4345..4704
                     /locus_tag="OO572_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6662305.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4744..5844)
                     /gene="trmA"
                     /locus_tag="OO572_003423"
     CDS             complement(4744..5844)
                     /gene="trmA"
                     /locus_tag="OO572_003423"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6662306.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5958..6148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6213..8057
                     /gene="btuB"
                     /locus_tag="OO572_003424"
     CDS             6213..8057
                     /gene="btuB"
                     /locus_tag="OO572_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6662307.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8002..8859
                     /gene="murI"
                     /locus_tag="OO572_003425"
     CDS             8002..8859
                     /gene="murI"
                     /locus_tag="OO572_003425"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6662308.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2067 a         2196 c         2444 g         2357 t
ORIGIN      
        1 tctaccgtta tcggtcaggc tatcgcgcct cctgctcttg tattgatgca gctggcatgg
       61 ggctggcgga cgatgttcgt tatcatcggc gtggcaggga ttctggttgg gatctgttgg
      121 tacgcgtggt atcgcaaccg ggcgcagttt gtcctgactg acgaagaacg gacgtatctc
      181 tccgcgtcgg ttaaaccgcg tccacagctg caatttagcg agtggctggc gctgtttaag
      241 catcggacaa cctgggggat gattttaggt ttctctggcg tcaattatac cggttggctt
      301 tacattgcgt ggttacccgg ttatttgcaa gccgaacaag gtttcagtct ggcgaaaacc
      361 ggttgggtag cggcgattcc tttcctcgcg gcggcggtcg ggatgtgggt taacggtatt
      421 gtggtcgatc ggctggcgaa aaaaggctac gacctggcga agacgcgtaa aacggctatt
      481 gtctgcggtt tgatgatgtc ggcattaggc acgttgctgg tcgtgcaatc ttcctcgcca
      541 gctcaggcgg tagcgtttat ctcaatggcg ctgttctgcg tgcatttcgc gggaacgtct
      601 gcatggggac tggtgcaggt gatggtgtca gaaacaaaag tggcttccat cgccgggatt
      661 caaaactttg gcagttttgt ttttgcttcc tttgctccga tcgtaaccgg ttgggtagtg
      721 gataccacac actcgtttaa tctggcgctg gttatcgcgg cctgtgtgac gttcaccggg
      781 gcgctgtgtt acttctttat tgtcaaagat cgcattgagt aatcgttact atcaggctct
      841 cttgctgtgg gcctgtagaa taaaaaaaag cggatcctgg agatccgcaa aagttcacgt
      901 tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca agggttaaaa
      961 gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag ggataatcgt
     1021 tcattgctat tctacctatc gccatgaact atcgtggcga tggaggatgg ataatgaata
     1081 ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag
     1141 attcctgcca cgttagccag ccgacgctta gcggccaaat tcgtaagctg gaagatgagc
     1201 tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc
     1261 tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa
     1321 gccagcaggg tgagacgatg tccggaccgc tgcatattgg tttgattcct acggttggac
     1381 catacctgct accgcatatt atcccgatgc tgcaccagac atttccaaag ctggaaatgt
     1441 atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc aaactcgatt
     1501 gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg ttgtttgatg
     1561 agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa tgcgtactga
     1621 tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt ttgcgcgatc
     1681 aggcaatggg tttctgcttt gaagccgggg cggatgaaga tacacacttc cgcgcgacca
     1741 gcctggaaac tctgcgcaac atggtggcgg caggtagcgg gatcacttta ttgccagcgc
     1801 tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccatgc attaagccgg
     1861 aaccatgccg cactattggc ctggtttatc gtcctggctc accgctgcgc agccgctatg
     1921 agcagctggc agaggccatc cgcgcaagaa tggatggcca tttcgataaa gtgttaaaac
     1981 aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatcgt cgggtagtta
     2041 aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc cataatcgcc
     2101 tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct
     2161 tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac gatttgtgca
     2221 cgtgccagat gtttgaactg ggcgcggccc acttcatacg gcactttcat cgcggtcagc
     2281 tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc ggtagggata
     2341 tcttcaatca gatgtgcggt ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc
     2401 tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac cgcgtaaacg
     2461 tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcgact gtcagtttcc
     2521 agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc
     2581 aggcagtcag ctttcagttt tttacccgac ttcagatgca tgatcacacc gtcgtcacag
     2641 ctttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt ccagaagtgg
     2701 taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg
     2761 atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca gccgatcact
     2821 ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg
     2881 tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg agagccgcag
     2941 gcaataacaa atttttcagc ggttagtgtt tcaacgctgc cgtccgggca atccagcgcc
     3001 aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg attacgttcg
     3061 taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg gttaaggata
     3121 tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt atagtgggtt ttgattgaat
     3181 tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg
     3241 caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcgcacc ttgcttaacc
     3301 aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc atcgtaatcg
     3361 taggaatgtg gcatggtagg gcttacctgt tcttatacat aaaagcaaca gaatggtaac
     3421 attctatcgc gggtaagcca attgatcccc gtcatttatc tggctatatc ctgagcggcc
     3481 tttgctttgt ctgtttctta cttttgccct gacgttttat tggattttta tcgacgatac
     3541 tctccgttta agcggcaggt ttccgctgta cgtaaaagaa ccggccagta aatatgtttt
     3601 attgcgttac cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag
     3661 tgccagcagt acttttggca aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa
     3721 agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag
     3781 cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt
     3841 ttatcggcat ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt
     3901 aatgctacgc caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat
     3961 ccgtacctcg gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt
     4021 attattgcgg gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat
     4081 tcagcacttc attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc
     4141 gtttactgaa gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc
     4201 gttggacgtc ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg
     4261 aatgatttcg aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat
     4321 tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg
     4381 ctggcgttgg ttgctggttt atcgattaat ggtactttcg cagcgctgtt tagctccatt
     4441 gtgccatttt ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa
     4501 cgttatctta atcgcaccat gtcggtaggc ttgccgggtc tggcagctgc ctgttttatt
     4561 ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac
     4621 ttcttcccgg cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac
     4681 cgtaagcagg aagttgctga gtaatcggta tcatgccgga tgctggcgca tccggcatgg
     4741 gttttacttc gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc
     4801 aaacagagcc agacgttcga ccttgtgcgt ctggcttaat gtttccagat ttttgcataa
     4861 cgtttccgga ttgcaggaga tatacagaat acgtggatac gcctgcacca ttttctcggt
     4921 ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg gtttcgaact gatagctctt
     4981 taagtcgatc ccttgcaggc ggttaaactc gcgcacacca ttcatcgcct gagtaaattc
     5041 ttctgccgcc atacgaataa tttgtacgtt atcaatatgg ttagcggcaa tgttgtattg
     5101 cgcggcagcg actgacggtt tggcgatttc ggtggctaat acccgatcaa aattgcgcgc
     5161 cagcgctaat gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc
     5221 tttggttacg tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt
     5281 aaagctgttt tctacctgac gatagatcat ctctttccct gcgaccggca gacgctcgtc
     5341 gatgtaatct tgatccagcg cgattttggt tttcgttgcc cgaccaatca gatgcacatt
     5401 cagattctgc gcgcgcagtg catcgcgcag tgcttccgct tcctgacgcc actcatcatc
     5461 cagcttttta tggtacagca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc
     5521 aatctggaac aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt
     5581 catcaactgg ttgatgagtt cactggcggc ggggaagcta tccacgcgga tgcggctttt
     5641 ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc
     5701 cgcgcgcatc cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa
     5761 cggtgccatc atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc
     5821 tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt
     5881 agggattgct catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga
     5941 cagtaacttg ttacaacctg tagcatccac ttgccggtcc tgtgagttaa tagggaatcc
     6001 agtgcgaatc tagagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg
     6061 acactgccat ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg
     6121 aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg
     6181 cggcatcctt cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg
     6241 cgtgttccgt cacggcattt tccgcttggg cacaggatac cagcccggat actctcgtcg
     6301 ttactgctat ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga
     6361 cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc
     6421 cgggcgtcga tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg
     6481 gtacaaatgc cagtcatgtg ctggtgttaa ttgatggcgt ccgcctgaat ctggcggggg
     6541 tgagtggttc tgccgacctt agccagttcc ctattgcgct tgtccagcgt attgaatata
     6601 tccgtggacc gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca
     6661 tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc
     6721 agaactatga tgtctctacg cagcaacaac tgggggataa aacacgggta acgctgttgg
     6781 gcgattatgc ccatactcat ggttatgatg ttgttgccta tggtaatacc ggaacgcaag
     6841 cgcagacaga taacgatggt tttttaagta aaacgcttta tggggcgctg gagcataact
     6901 ttactgatgc ctggagcggc tttgttcgcg gctatggcta tgataaccgt accaattatg
     6961 acgcgtatta ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt
     7021 gggacgccgg gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata
     7081 gccatagcaa agattacaac tacgatcccc attatggtcg ttatgattcg tcggcgacgc
     7141 tcgatgagat gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta
     7201 gtattggtgc gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg
     7261 aggatggata tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg
     7321 attttacctt tgaaggcgca gcacgcagcg acgataactc acagtttggt cgtcatggaa
     7381 cctggcaaac cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg
     7441 ggacatctta taaggcacta aatctggggc aactgtatgg cttctacgga aatccgaatc
     7501 tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga
     7561 actggcgtat ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca
     7621 ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg tgtcgaggcg acagccaatt
     7681 ttgataccgg accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga
     7741 ttaccgacac gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc
     7801 agttgtatga cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg
     7861 attactcatc ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg
     7921 cggttgcgta tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg
     7981 acaaagatta tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg
     8041 gcagctacac cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg
     8101 ttgtcggtct atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc
     8161 gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg
     8221 gcaattgtca ccgcggtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac
     8281 accgccagta ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt
     8341 gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg
     8401 gcaacccgcg gaacagttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat
     8461 gaatgccaga tagaaatgct gggctcggca gagatggttg agttggctga agcgaagctg
     8521 catggcgaag atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg
     8581 aaagagccgc cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa
     8641 ctgttacaag tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc
     8701 cgaacggcct ggttgttaga acatgaagcc ccggatgcaa aatctgccga tgcgaatatt
     8761 gccttttgta tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac
     8821 ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt
     8881 cggaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
     8941 actgacacgg aacaacggca aacacgccga cgggtcagcg gggttctcct gagaattccg
     9001 gcagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgtacacccc
     9061 gcgc
//
LOCUS       DAJTYL010000138         9019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYL010000138 DAJTYL010000000
VERSION     DAJTYL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.138"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO572_003426"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO572_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6662309.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO572_003427"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO572_003427"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6662310.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO572_003428"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO572_003428"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6662311.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO572_003429"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO572_003429"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6662312.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4456..5580
                     /gene="dprA"
                     /locus_tag="OO572_003430"
     CDS             4456..5580
                     /gene="dprA"
                     /locus_tag="OO572_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6662313.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5552..6025
                     /locus_tag="OO572_003431"
     CDS             5552..6025
                     /locus_tag="OO572_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6662314.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6054..6596
                     /locus_tag="OO572_003432"
     CDS             6054..6596
                     /locus_tag="OO572_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6662315.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6601..7173
                     /gene="tsaC"
                     /locus_tag="OO572_003433"
     CDS             6601..7173
                     /gene="tsaC"
                     /locus_tag="OO572_003433"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6662316.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7178..7996
                     /gene="aroE"
                     /locus_tag="OO572_003434"
     CDS             7178..7996
                     /gene="aroE"
                     /locus_tag="OO572_003434"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6662317.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7993..8250
                     /locus_tag="OO572_003435"
     CDS             7993..8250
                     /locus_tag="OO572_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6662318.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8226..8780)
                     /locus_tag="OO572_003436"
     CDS             complement(8226..8780)
                     /locus_tag="OO572_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6662319.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2190 c         2251 g         2324 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag gcttgcttat
     4441 ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag catcagcagc
     4501 ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc gcatattgat
     4561 gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
     4621 ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
     4681 cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca
     4741 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
     4801 cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac tctggcgacg
     4861 tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt gcataaagcg
     4921 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
     4981 ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc tctcgtctcg
     5041 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
     5101 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
     5161 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg
     5221 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
     5281 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
     5341 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
     5401 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
     5461 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
     5521 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
     5581 aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
     5641 taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc tggaaaaact
     5701 tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc ctctctccat
     5761 gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg ggtttctgct
     5821 tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga tagagcgagt
     5881 gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga tcctgatggt
     5941 gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
     6001 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcaa
     6061 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
     6121 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
     6181 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
     6241 tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
     6301 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
     6361 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
     6421 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa
     6481 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
     6541 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
     6601 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
     6661 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
     6721 acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
     6781 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt gactgacgtg
     6841 cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt tcccgcgcct
     6901 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
     6961 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
     7021 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
     7081 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
     7141 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
     7201 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
     7261 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
     7321 cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc
     7381 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag ttaataccct
     7441 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
     7501 cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta tcggcgctgg
     7561 tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
     7621 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
     7681 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
     7741 atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat
     7801 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
     7861 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
     7921 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
     7981 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
     8041 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
     8101 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
     8161 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
     8221 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
     8281 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
     8341 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
     8401 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
     8461 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
     8521 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
     8581 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
     8641 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
     8701 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
     8761 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
     8821 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
     8881 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat acttgtgcaa
     8941 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacagga
     9001 tggtcggaac aacgaagag
//
LOCUS       DAJTYL010000139         9006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYL010000139 DAJTYL010000000
VERSION     DAJTYL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.139"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..717)
                     /locus_tag="OO572_003437"
     CDS             complement(<1..717)
                     /locus_tag="OO572_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6662320.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTP"
     gene            777..1055
                     /locus_tag="OO572_003438"
     CDS             777..1055
                     /locus_tag="OO572_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662321.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1056..1583)
                     /gene="gadE"
                     /locus_tag="OO572_003439"
     CDS             complement(1056..1583)
                     /gene="gadE"
                     /locus_tag="OO572_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6662322.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2382..2954)
                     /gene="hdeD"
                     /locus_tag="OO572_003440"
     CDS             complement(2382..2954)
                     /gene="hdeD"
                     /locus_tag="OO572_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6662323.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3209..3541
                     /gene="hdeA"
                     /locus_tag="OO572_003441"
     CDS             3209..3541
                     /gene="hdeA"
                     /locus_tag="OO572_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6662324.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3657..3983
                     /gene="hdeB"
                     /locus_tag="OO572_003442"
     CDS             3657..3983
                     /gene="hdeB"
                     /locus_tag="OO572_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6662325.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4047..4693
                     /locus_tag="OO572_003443"
                     /pseudo
     CDS             4047..4693
                     /locus_tag="OO572_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4735..5265)
                     /locus_tag="OO572_003444"
     CDS             complement(4735..5265)
                     /locus_tag="OO572_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6662326.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5421..5987)
                     /gene="slp"
                     /locus_tag="OO572_003445"
     CDS             complement(5421..5987)
                     /gene="slp"
                     /locus_tag="OO572_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6662327.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6234..7457)
                     /locus_tag="OO572_003446"
     CDS             complement(6234..7457)
                     /locus_tag="OO572_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662328.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8038..>9006)
                     /locus_tag="OO572_003447"
     CDS             complement(8038..>9006)
                     /locus_tag="OO572_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6662329.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2640 a         1778 c         1875 g         2713 t
ORIGIN      
        1 tggcgtactg ccctgaacct gtttgatttg cccactggcg acctcttctt tcatgcgtaa
       61 gaaatcttgc accgactgcg tgagatcgac ataaatcggg tccagacgtt gtacggtaac
      121 cagcgaatct gcctgattag cggtaacgag tgcgccgacg gtcaccgacg atttcccgct
      181 gacgcccgta atcggcgagg tgacattcgc gtattgcaga ttgatcgtcg cctgttcaac
      241 agccgctttg gcgacggtga cattggcttc tgcttcattc agctgggtgc gcgcggtgtc
      301 gtaatcctga cggctaacgt agttggtctt cagcaacgat gcctggcggt taaaggtgat
      361 gcgggcattg ctggcggtag agagcgcttt cgccagcgag cctttggcgg agtttagctc
      421 ggcctgtaaa ggtgcaggat caatctgata cagcgaatcg ccctggttca ctttatcgcc
      481 ttcgataaag ttgcgtttaa tgataatacc gcccacctgg ggacgtatct cggcaacttc
      541 ataaggaacg gttctaccgg gcaattcgct caacacattg accgaaccag gggagagtgt
      601 gacgacaccg acctcaggcg tcatggcggc ggcgttttcc gccgatttgt catcgcaggc
      661 ggtgagcatc gcgccgcaga ataacaacgg tattaacagc tttcttcttc tgttcatttt
      721 agtccctgaa aattcttgag aaataacggt tctttaaatt gcgcgcgagg agagatatga
      781 gggatttaca gaccagcggc atagtggggt tgtcagccag taaggtggga tacaggcaca
      841 gtgatcgaca tggtgaggtc aacgacaaaa aaaatgttgc cgttctgcca acagttcctg
      901 ccagcattcg ggcaacgaag ggttctactg gtggatacac ataccagggg aatcaagtcg
      961 ttttagcctc tcctttagta atcaccgggg gtaatgaaat cagcatctgc atccctcgtc
     1021 atgccagcca tcaatttcag ttgcttatgt cctgactaaa aataagatgt gatacccagg
     1081 gtgacgatgt cgctcatacg tcttagtttc agcttgtaca taatgttgaa tttctgaatc
     1141 tttaatgttt gttgcgaaat attgagcgtc gacgtgatat tgctttcatt tttatttttc
     1201 agaatcagtt gcataatttt gaactgatta tgtgaaaaac ataattgtga attcttatgg
     1261 ggcaagtgtt taccataagt aatctcaaga agatttttga tactttcttt gcggctaaca
     1321 aaataaatag gcgctttagc ttttagttta ctgatgtgat aaggtgccaa aacaataatt
     1381 ctctcggcat ctaatttctc cagaaattta atcgcttctt catcaaggat atgattgtga
     1441 taggtatcaa tgataacctt gaagggttta ttgcctactt ttttgatctc tgacagggaa
     1501 ttgattttta tcatttcgtg attatctttc aactgccaaa agccctgtaa aagaaaagaa
     1561 tctttcgtca tgagaaaaat cataacttgc tccttagccg ttatcgtttc gaacatgtca
     1621 tccttttcat tatttacatc cttgtccgaa tcgttgttca atatagtaaa cgcctataag
     1681 aactttgccc ttcgtgaact aatacgtcta ttaacactaa tcaatacttc agatttatcc
     1741 atcacgcact ccatatattg atcctaagta agattcctgg ttgttatcag cttgtaaata
     1801 gtaaacaaaa agttaacgtt tgcggattac tgcccaagaa taagttatca ataaccagtt
     1861 tgtgatctct gaagaatatt actaaagtta aaatgtaatc cgatttaaat atcgagtctc
     1921 cttgtttcga cttaagctgg caattggatt gccagctttc ttttaacgca ttgaaattac
     1981 ctgagtaaat aacgcattca tgtcttcagg tttttatata gcgtctggag acacggatat
     2041 ttatgcaatg taatcaacgc cagttgcaaa aaaatgaacc cggattactt atttaaccgc
     2101 ccatagcatc aacgcttacg gaactctttg ttcactttcc cacagcactt ttaaaacatt
     2161 aaacctgact acggaaaata tcagccatga taatgtttga aatggctaat ttgccatgga
     2221 gtgaaaaaaa ttagatgaaa ttcagtaggt tgaaataatc actagcaggt aattatttca
     2281 atgatagtgc gcaattgatc tacaacactg cgtagcggag agagtattaa tcggatcata
     2341 gtcacatcaa gtgactatga tccgggtgac aaccggggta attattgctg cttaacgaac
     2401 aaactggcga agctgaacag gctggcggcg ctaaatatca gttcaattcc caccagtgtg
     2461 gaaaccagcg ttacagacac catcggcgtt gcaccaagga atatccaggc aatgacgata
     2521 tccagcacac caataacgag ctgtagccag ctgcctttca ttgaacgctg acgataccaa
     2581 ctcatcaggc gaataacccc tgcaacacag aacaaaccgg caataaatgc cgcaatggca
     2641 aaaatgccca gctccggtgc acggatgaag aaatagccga tcaataaata ggcgactgcg
     2701 acgaggaaac cggataatac cggccagaaa ttatgactgc ggttgctgaa taacccgaca
     2761 ataagcgcaa tacccgagca gattaataat gcacccacta ctgtgcttaa aatatcgcca
     2821 gagacgaacg ggaaactgat acacagcaac ccgacgataa acagcagcac ggcaataaac
     2881 tggattgctc tgcgatgctt tttcagcatc tccagatcaa acttcaaaat tgttgcctta
     2941 tctatatata acatagaacc accctataaa attaagaaga aaatcccctg ctatcaatct
     3001 atgccaaaaa cgcgtctaag aatgcagtcg atttaataaa aatttcctaa ttgcagtatc
     3061 tgatgcatct gtaactcatt gtattgaaat aaaaatatct gattttgata ttttccatca
     3121 acatgacata tacagaaaac caggttataa cctcagtgtc gaaattgatt cgtgacggct
     3181 ctttcacttt atagttgagg atattacgat gaaaaaagta ttaggcgtta ttcttggtgg
     3241 tctgcttctt ctgccagttg tgagcaatgc agcggatgcg caaaaagcag ctgataacaa
     3301 aaaaccggtc aactcatgga cctgtgaaga tttcctggct gtggacgaat ccttccagcc
     3361 aactgcagtt ggttttgctg aagcgctgaa caacaaagat aaaccagaag atgcggtttt
     3421 agatgttcag ggtattgcaa ccgtaacccc agctatcgtt caggcttgta ctcaggataa
     3481 acaagccaac tttaaagata aagttaaagg cgaatgggac aaaattaaga aagatatgta
     3541 attccgggaa tgcgttacat cgtacttcct tgcatattga acaggccgga atatcttctt
     3601 taaaagcagc tattcctcct gttcatatat aatctctata ttgaatgggt tacaaaatga
     3661 atatttcatc tctccgtaaa gcgtttattt ttatgggcgc tgtagcggct ttgtcactgg
     3721 tgaacgcaca atctgcgttg gcagccaatg aatctgctaa agatatgacc tgccaggaat
     3781 ttattgatct gaatccaaaa gcaatgaccc cggttgcatg gtggatgctg catgaagaaa
     3841 cagtatataa aggtggcgat accgttactt taaatgaaac cgatctcact caaattccta
     3901 aagtgatcga atactgtaag aaaaacccgc agaaaaattt gtataccttc aaaaatcaag
     3961 catctaatga cttgccgaat taatgaggta caagtaaaaa ggagtagcaa gttgagccat
     4021 cttgctgctc ctttttgcat ttttatatga cagcagaatt tattatacgt cttatactcg
     4081 cggcaattgc ctgtggcgct attggcatgg aaaggcaaat gcgcggcaaa ggagcagggt
     4141 tacgcacaca tgtattaatt ggcatgggaa gcgccctgtt tatgattgtt tcgaaatatg
     4201 gttttgctga cgtgctgtct ttagatcacg tcggactcga ccccagccgt attgctgctc
     4261 aggtggtgac gggcgtcggg tttatcggtg caggtaacat tctcgttcgt aaccaaaata
     4321 ttgtaggtct gacgacggca gcggatatct gggtgaccgc cgcataggta tggttattgg
     4381 cagcggtatg tacgaactgg gtatttatgg ttcagtgatg accttgttgg tgctggaagt
     4441 cttccatcaa ttaaccttcc gcctgatgaa caaaaattac catttacagc tgacattagt
     4501 gaacggtaat acggtatcaa tgctcgactg gtttaagcag caaaaaataa aaactgattt
     4561 agtttcgttg caggaaaatg aagatcatga agtggtcgcc attgatatcc agcttcatgc
     4621 cacaacgttg atagaagacc tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt
     4681 ttcaattagc taatctgtgt ggcagcgtag ccagactcac cgtaagcctg aaattcacac
     4741 cagataatca atatgctgat ggcgtaccag ctcattgatc cgcttaagat tgaggatcat
     4801 catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat
     4861 atcttcagtt gtcatttgat tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag
     4921 tgacacattc gcttcaggca cagagcagta actgccattg atgagtgcga caaaatcaat
     4981 gatagtgata gtccgcggaa caaaggtcac cggtactttc cccagacagc gtttaatatt
     5041 aaagggggag aaaacaatga catggacagg ttttaatcgt tcaatttgag tgagaaaatt
     5101 agaatgaaat acattattca ttaatgtatc aataatgacg aaggcattct gatgcaacat
     5161 tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt
     5221 catcgcggtg aagaacatcg tatccctggt aattataaga aacataaccg actcctggtg
     5281 cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg cttataataa
     5341 taatagttag cttactgtat attaatttta tgtaatacat gatgcagatg ctgagaaagc
     5401 atctgcatct ttcggtggtg ttatttgacc agctcaggtg ttacctgact caccgcattg
     5461 gtgtagtaag gcgcacccca gcccggttcc ggccagaatg caccatagcc gtaatcccac
     5521 aggttatagg tggtattaac cacttctctc aaatgccaca cctggatgcc ctgcatattc
     5581 acttccagga agttatacgg gactttattg ataaagcctg gttgttcacc ctgaatggta
     5641 ccgaggatgg taacaaagtg attacgatag ttcactggat caaggaagcc gctttgtctg
     5701 gcgagcagtc ggccctgata gttggcttca atatcaggct tcgcatagct atccaacggt
     5761 aatacagcga tttctaacaa cgtatccgtt ttgccattga taacgttgat aaccttccca
     5821 ccaaagcgcg cttgttgacc aacatataac cccggctggt tatgaacagc aacaaaactt
     5881 ttttgaatat caggttggtt attgcctttg atattttgcg gaattgaact acatgcggca
     5941 agcaaaaatg aaaggctgag gatgagtgca ccttttgtca tgttcatgtt actatcctta
     6001 tcaacaaata ttaaaaccat aatagatatt tctttatagt tttcatctga ttctgaggtg
     6061 atatgcctga tagtaacgta aacctgtctg atgtttatat cccttctgtt tagttcaatt
     6121 ccgttatttt ggggcgtggt atgagtatcc atcgcccaat aatataaccc tttgttttaa
     6181 aattacataa aatctatttt tattcaatga ttataaagtg ctaataatca atattatggt
     6241 attatattct gataatgatt tgataagcga attcttttgc cagatggtat ttctatttcc
     6301 accccataat ttatatcttt ttcattaggc agatctctct ttaaataaaa ggggtatgaa
     6361 atcttttcgg gttgaagtgt ctcgtaatga aattctatga attcattttt agacattgtt
     6421 gcccattttt gaattttatt gtatagagag ggtgttgtaa actcttgaag ttgtttataa
     6481 catatttttt tgaactcttg cttatatttt aatatgtcat caaatgaaag attcactacc
     6541 tttgcctcta agaaatcaat tattctcgaa gcaatttccg ctttcgcgcg taaagagttg
     6601 tcattaatag ttaacaatgt agaaaacaaa ccattgattt gtttgtcaag atgttgttca
     6661 tatttatgta gtttggggat gggattttcc ttgtgtgaaa aaatagtgtt aaataaattt
     6721 gcgtcagtga aatttatatt aagtagatct tcaaaataaa attttgataa ggttaggact
     6781 agcttggaca tatcttgccc tgtcatgttg aaatcaataa agtcgcatcc agcaagggct
     6841 tccatcctat tttcattttt actgtgctca atatacagct tcgccatttt taagtacaag
     6901 ctttgaatgt ctcttatatc agttggtaag tcagctcgtt gcccagcaaa atttttatta
     6961 tcggaagaaa cggtttggac actgaatctt tcgggtattc cttcttctac gagaatttta
     7021 gttatgattg gattgttggg ttctggatta acgacaacaa tgatttgaca tacagaatcc
     7081 ttatcctttt cgtatctaat gttatgaatc tgattaagta ttgattgtgt ctgttgcgta
     7141 agtggtgtcg aacttatcgt ttcaatgtta ttaataagac ttacaagttg aaccacaact
     7201 tttgagtagt ctgcatttgt atatgtggtg ttcgtgtggc atgcattaat ttgttggagt
     7261 tgttgttgcg ctaaatcaaa ttgttcaggg ttgtatcctt ctccagagaa tgtggaatta
     7321 atgggttgtt gttcttccca attatcacaa ggccacttcg gtgtgtctgg cgttttaaca
     7381 ttatcggtat ttgttgtgca gttataaata tcgccgtgat atgtatttat tatacctggg
     7441 gtaaagtcaa ttgacataac attccttatt catcatctgt tttatgctaa tgtttttaaa
     7501 ttggataatt agaacaggaa tggattttct tctacacaca ataaatataa tgcgttatat
     7561 cttcctgata taattacctt taaattccct ttcatttcca cccggtaata tttgtgtata
     7621 aaaaaagggc agtgatagta tcgtaaagga aaacgttaac aactatcaaa aaacgctcat
     7681 tttaaaaagt tattatgttc tccgatagtg tgtgaagtaa tatttttgtg atgttttaga
     7741 tgtgtaataa tgaaccaggt taaagtgtat ttaaaaatgt gttgtattag cgactgaaat
     7801 ctgcctttta aaaaggagga gggtactgag acaaagagtc attgcttctc cgataaaatg
     7861 tcagagaagc atgattacgt ggttattgtc tgttgatgac ttgttgaccg agccgtcatc
     7921 attctcaatg cattagttaa atcatttgtg atttttaagg attatcctgt ctatgatttt
     7981 ctacttagaa ggatatggca ctaactatgc atcttatagt ttactgctgg catctattta
     8041 aaatagcaat tgagtcaaat agcccatcaa gatagccata cctaatataa ccgtcaggaa
     8101 tgcagctatc atcggcattc tgaacattga tttaagtaat atcacttccg tcaggcttgc
     8161 gccggcactg ccgatgatta atgccattaa tgctcccaga cccattcctt ttgtcatcag
     8221 aacagatgcc agagggataa ccgcctcagc ccggatatac agcggaatac cgacaacggc
     8281 gcttaatggg atggcgaagg gattatctgc tcctgcatga gcggcaatcc acgctgaggg
     8341 aataaagcca taaataaaag atcctattaa tactcctaac aaaagatatg gcagtacatc
     8401 tttaaactgt tgcaacgcat ctttggtggc tagttttatg ggattaatac ttactacagc
     8461 tttggtactg caacaggtaa ctacggggtt ctcaatggct ttagccgctg gactacaaca
     8521 actcactttt atgggcgtat atgtcgttcc cggcgaagtt tttgctggag cacaacaatt
     8581 tgctgatgag cttttactgg caatgatatg acgttcaaac cccagagaat ccaggataat
     8641 actggcaaga acggagacgc cggctgcgat aatcgcgtac aacaaggtga ctttccaacc
     8701 aaaggtcatc cacattagtc cgacgataat gggattaagt aatggtgaaa caaataaaaa
     8761 agtgagagtc ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca tgggtattgt
     8821 cgagcaacta cagaacgggg taacggctcc caacagagca gccaggagat agcctctccc
     8881 ttttctggcc cccatcatct gctggatttt atggtctggc accttttgtc ttatcaggct
     8941 gacaccggca ctaatcacta taaacagcaa cgaaagctcg acggcgagaa acacaaacat
     9001 ctctgc
//
LOCUS       DAJTYL010000140         8940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYL010000140 DAJTYL010000000
VERSION     DAJTYL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.140"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..155)
                     /locus_tag="OO572_003448"
     CDS             complement(<1..155)
                     /locus_tag="OO572_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="iron-sulfur-binding subunit; with XdhA and XdhB
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase"
                     /protein_id="HCR6662330.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGA"
     gene            complement(152..1030)
                     /gene="xdhB"
                     /locus_tag="OO572_003449"
     CDS             complement(152..1030)
                     /gene="xdhB"
                     /locus_tag="OO572_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6662331.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1041..3338)
                     /gene="xdhA"
                     /locus_tag="OO572_003450"
                     /pseudo
     CDS             complement(1041..3338)
                     /gene="xdhA"
                     /locus_tag="OO572_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            3753..4508
                     /locus_tag="OO572_003451"
     CDS             3753..4508
                     /locus_tag="OO572_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6662332.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4587..4660
                     /locus_tag="OO572_003452"
     tRNA            4587..4660
                     /locus_tag="OO572_003452"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4619..4621,aa:Gly,seq:ccc)
     gene            4795..6405
                     /locus_tag="OO572_003453"
     CDS             4795..6405
                     /locus_tag="OO572_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6662333.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6437..7044
                     /locus_tag="OO572_003454"
                     /pseudo
     CDS             6437..7044
                     /locus_tag="OO572_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7975..8721
                     /locus_tag="OO572_003455"
     CDS             7975..8721
                     /locus_tag="OO572_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6662334.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2291 a         2190 c         2291 g         2168 t
ORIGIN      
        1 caggcaccac attcacccac gcagcaccct tgtttgacac ttagcagtcc ttgttcgcgg
       61 agtaattccg agagcggcgt gcctggtgcg gcgtgaagct gaaaaggcat cccgttaatg
      121 gtgcattcgg tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc gccgcggcga
      181 cggcttcgct aatcactttt ttggtcatcg tctggatgag atgcagacga aactctttac
      241 tggcccgcca tgaagaacgc ggggcgacat cttgcaggac agattcgctg atagcttcca
      301 gcgtttgcag gtttaatggc gcattttgtg cagtctgttc ggcatgttgg cagcgaatcg
      361 gcgttggcgc ggcaacacca aatgccaggc gtaattcgct gaaattgccg ttatccagtc
      421 ggcaatgtgc ggcgcagcca atcgttgaaa tatccattgc gtcgcgcatg gcatatttaa
      481 aatgcgcgct gcccgcgtgt tctttcggct gtggcggaaa atgaaaggcg acgaggattt
      541 cgtcatgctc aagagacact ttgcccggcc cggtgtgaaa gccattaatc gggacgaaac
      601 gaacaccgcg cggggagtgg atctccagtt tcgcgtcata aattagcgtt ggcgtggcag
      661 aatctgcgct ggtggcaccg ttgcaaatat ttccaccgta ggtagcgacg ttacggatct
      721 gcggtccagc aatgaacgtg gccgcagcac ataacgccgg gagatgacgt tgagttatag
      781 gatcttctat tagctgggta aatgtcgttg cagagccgat acgtagcgag ccatcttccg
      841 ccagcgtaat tccccgcagc tccgccagat tatgaatatc aacaatatgg cgataacggt
      901 cattgtgatg gtggagctga atcagtacgt cagtgccacc ggcgagcagt ttggcctgcg
      961 ggttgtcagc cagcaggttg atggcatcgg caagggttgc tgcgcgatgg taagaagcaa
     1021 aatcaaacat gatgttatcc tcaaatcaat cctgccagat ggaactcttc atataaccgt
     1081 tttggcgtca gcggcagtgt attgattgca acaccggtag ccatcttcac cgcgttacga
     1141 atagcagcgg caacaggaat tatgggtggc tcacccagtg acttatgtcc gtatgcggat
     1201 tgcggctcat tgatttcgac gaacgcgctt tccagttgtg gcagatccgg catggtcggc
     1261 attttgtaat ccagcagatt ggggttacgg accacgccgc ttttagcatc gatgatcatc
     1321 tcttcaaata gcgcccagcc aatgcccatt cccattccgc cgtgtacctg accttctgcc
     1381 agcagtggat taagaatatg ccctgaatca tgaacgttga ggatgcggtt gatggtgact
     1441 ttgcacagcg caatatcgac cgtcagatca acaaaggtac agccaaacgc cggtgggtta
     1501 gtggtggttt tgatggagct ttcagcagag agctgcccgc cgcgttcagg gtggtagaaa
     1561 gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc aaccagcacg
     1621 atatggcctt ttatcagggt caaattcatc gctgactgat gtagcatgac tgcggcgtga
     1681 gcgatgattt tctcttttaa taatagtgcc gcactgcgca gcgcaggcgc ggcaacatag
     1741 ctctggcgtg aggcaaatgc gccgggatcg aacggcgtaa cgtcggtatc ttgtgttgaa
     1801 ataacgcgaa cgtcgctgac cggaaccccc acggtttctg ccaccatttg cgagaagacg
     1861 gtgtcggcac cctgaccgat ttccgtcgcg ccgctttgca cgttgatggt tccatcctga
     1921 ttcatcagaa ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt agaggtgtag
     1981 ctaaaacagg cgacgccaac gccgcggcgc aaattgcctt gctggttctg gcattctgca
     2041 cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg caaccctgcg
     2101 ctgcaaatac gtttgcccgt gagcggatta gcatctcctt cgcgggcggc gttgcgtaaa
     2161 cgaatttcaa caggatcaat acctaacgct gtcgcggcgt catcaagcat agactcaacg
     2221 gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg gaggttggta
     2281 tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc gactttattc
     2341 ccccccgcag aagcgatgga gtgcccgtga gatgcataag cgccggtgtt agacagaaca
     2401 tccagactat aacctttcaa ggttccgtcg cggttcacgc ccatttgccc gtcaatagta
     2461 aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga aactttcacc
     2521 ggaatgccgc caagcttgct ggtcaggaat gccgccattg gctcttccag tacatcctgt
     2581 ttattaccaa aaccgccacc gacaaacggt ttgatgactc gtacgcatga ccagggaata
     2641 tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct ggaaacgatg
     2701 gtaattcgcg agtcatcctc catccacgcc agcgatgtta cgctttccat gtgacaatgt
     2761 tgaataacgg gagtctgata gtgcccctgt acctggtagt cggcggcatc gattgtttgt
     2821 tggacattgc ccgtcgacat cgtgctttgt ttcagtaaat tgccgccgtt atggattggt
     2881 gcagcgtctt ctgtcagcgc cgcttctggc gtggtgataa cgggtaattc ttgccactca
     2941 atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac gacgatggca
     3001 acggcgtcac catgatgacg aacatggcga gttagcagtg cgcgatcggc ggtatcgcgc
     3061 ttgttttcgt caagtgtcta ggcatgccca gctgtagcga atggaatatc aggcacatct
     3121 tcccaagtaa aaatcgccag tacgcccggt aaacttctgg cttgttcatc attaatactt
     3181 acggcataac catgtgcgat agggctacgt acatatttcg cgtaacacat gcccgccata
     3241 acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc gacgcgcatg
     3301 catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc gatttgaaca
     3361 tttggttctt atggcaaaga tttagtctaa gcaggcaaga tttacgccag aaacgacgat
     3421 aagcgttagg ggaggaagga agtgtgatga cgttaaaaaa acacagatat gatacgaaac
     3481 aggactctga atagggacag tgatacgtat caatatgagt ggcgataaaa tcaatgtgat
     3541 aaatcaggat tattacgcat caggatggga aatagggtaa aaagtcagga acttcgatac
     3601 ctctacagcg aatattttgc tgatgatact tccctttgcc aaatgaaatt aacctctttc
     3661 tctattacca cgtttttcca gaagcaagag attgggtatc aagaggctgg ctgctatgat
     3721 atggcgtctt gttttttaag cgaggaaaga ttttgagtgc gggacgcctg aataaaaaat
     3781 ctctgggtat cgtgatgttt ttatcggttg gactgctatt ggcgggctgt tcgggtagca
     3841 aatcatccga tacaggaacg tattccggct ccgtttacac cgtgaaacgg ggggatacgc
     3901 tatatcgtat ttcgcgcacc acgggaacca gcgtaaaaga gctggcgcga ctgaacggca
     3961 tttccccccc ttacaccatt gaagttggtc agaaactaaa actgggtggg gcgaaaagta
     4021 gcagtagtac acgtaaatca accgccaaat caacgactaa aaccgcatcg gttacaccgt
     4081 catcagcggt accgaaatct tcctggccgc cagtagggca acgttgttgg ttatggccaa
     4141 cgacagggaa agttatcatg ccgtattcga cagcagatgg cggcaataaa gggattgata
     4201 tctcagctcc acggggtaca cctatttacg ccgcgggtgc aggaaaggtg gtgtatgtgg
     4261 gcaaccagct gcgtggctac ggtaatctca tcatgattaa acacagtgaa gattacatta
     4321 cggcttacgc ccataatgac acgatgctgg taaataatgg gcaaagcgtg aaggctgggc
     4381 aaaaaatcgc cactatgggg agcacggatg cggcatctgt tcgcctgcat ttccagattc
     4441 gttaccgtgc aacggcaatt gatccgctac gttacttgcc gccgcagggc agcaagccaa
     4501 aatgctgatg gcgaattaat cagcagtcag cagcgcggca cttgctaagg agagcgtaag
     4561 gtttataatg ccttacgcat ctcgaagcgg gcgtagttca atggtagaac gagagcttcc
     4621 caagctctat acgagggttc gattcccttc gcccgctcca tgacacttct taaagtctac
     4681 taacttactg aatcctaagg gatacggctt aacgccttcc gatagtttta ctcactgtaa
     4741 ccaacactgt gactaacttt gttgaagtag tgagcagacc taaggtctca gtacatgctc
     4801 aagtcaagaa cctaccttta tcagcgtaat ggtgtctttt acatccgtct taggatgaag
     4861 accactagtc gcctgaccgc ttcgcttcct tctcacaacc gttataaact agcgtcagta
     4921 tctttacgga ccaaggatag acgtaccgct atggctcact cacggcacat caagtcagca
     4981 cttaaagcaa tccatgcaga caaccctaac gcctcttatg aggagctacg ggagcacctg
     5041 aagaccattg tcgagtggga acttagtgtc agccgtgatg acctgaacga cccggagtcc
     5101 taccagcttt acgttgacca gtacgatgac attaagtcca accttcggga agctgtagcg
     5161 accgagcgtc tgactgtaga ccagcatcgc tatattaatg acgttatcgg tgtgcttaag
     5221 gcctgtcagg atagactcaa tggcgatagc tctggcctat tgtcttacct tgagcctgag
     5281 actggcagtc taagaccgtc cgtctcccta tctgtattgg cggaacctga agtccctgaa
     5341 cctaaagcac tgaccctcgc gtctctcatt gagcagtacg agcaggagaa cgcccagaac
     5401 tggaagccag cgaccctaag cgaaaaccga gcgtcacact ctacgctaat cgagatattc
     5461 gactatctgg atattcagga tgtcggcaag gcgacccgtg cagatatgct cagagttcgt
     5521 gaagtcctcc agcagctacc gaagaaccgc aagcaacgct ttaagtctat gccgctgtct
     5581 gacttgctga accgggagtc taagactgac tgtctggacg tcgttaccat taacaataaa
     5641 tatcttatca agatggctgc tgtgtttaag tgggcggtac gcaacgactt gatagctaag
     5701 aacctgaccg aaggtcttga gctgaaagtc cctcagcgta aagcctccga cgctcgtgat
     5761 gcgttcagtc ctgagcaggt agggcaacta ctggtcgcag ctaaagcgta ctcgcagaag
     5821 acctcaggta agccatacca ttattacgtc accgctctgg ctgcgattac aggtgcgaga
     5881 cttaacgagg tggcccaact tcaggttaaa gatgtcagga ccactgaggc tgggaccgtt
     5941 tttatccata tcaacgaaga cgatagcagc ctgccgggta agagtatcaa gaacgcccac
     6001 agtgaccgct gtgtcccgtt ggtagatggt gcttatggtt ttgtattggc tgactttatg
     6061 tcactcgtgg aagaccgtag aaagactgag ggtgataatg ctatggtgtt caatggcctc
     6121 aagctaatga agaacggcta cggtgagcag gttagcaagt ggtttaaccg aacgttgctg
     6181 cccaaagtcc tcgctgaccg tagcggctta gcttttcact cgtttagaca tacagttgcg
     6241 acccagttaa aacaacatgg ggtcgagtta gcgtatgctc aggcgattat gggtcatagc
     6301 tcagggtcta taacttacga ccgttacgcg aaagaggtcg aggtggaaac gctgaaagaa
     6361 aaattagcgg agtcattgtc agtaaaaaaa atagacggaa agtaatcgca tagggatagc
     6421 ggaatgtgag gacgtaatgg tcaaagaatt tttagttagc aaagaaaata agccttcata
     6481 tgaatgtatg aatactgctg aaacttgttt ggggctggct ggaatggctt cggaagattg
     6541 gcaacgtgag catcattgcc gcacaggtat aatatttact gcttttgcta ttgaggcaat
     6601 gttcatcttc tatagaaaac aggttgaccc cggctacgat aagacgcaga aagagtgcag
     6661 aaagacaatg cacaagaata cgttaaaatt gtgcggtata aacaattata tggggacaaa
     6721 accttaccaa attatcaaag agtgtcttga ggtaagagac gctattgctc acggcgattc
     6781 gtatacttcg agctttaatt tctcggcgga tcatttggat aaccaagacg atattgcaaa
     6841 agtgtcctgg cggtaaactc tgaacaattt cggggattaa ctgttgagcg ccttgcaaaa
     6901 tacattgaaa tggcaaagca tattgaccat gagatttctg ataatggtta caggccttct
     6961 gaaggttacc ttccagcagc cgagcgcaga agtctgatgt tagcttttgg cgtcagtggt
     7021 gtgtcaatgt ggcccagtgc ttgagcgata atacacctca ctatagagga atggaccctt
     7081 aactatcact ataggggaga gaccctaagt ccccacctta atagcagtca cgacccatac
     7141 acacattcat taagtctcag gcttccggta gcggaccgat tggaaaccat ccgcagacgc
     7201 ctagtgttaa atcaccttgg cctgagacca ttccttttaa cgtaagctat cttccctttt
     7261 gacaggcggg gagcagtaga ccacatccga ctttaagttg gctctgtgat aatcgctgcg
     7321 cggataactg aagaaccaag acaccattac tgaggtctgc catccatttg tggacaggcg
     7381 gaaactcaaa gaggttagat cagtggtgtc tgaggttgga gctttgagtc ttgaccagca
     7441 ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat tccaataaga
     7501 agaactttaa gtccagcctc cactcccgcc aacccgaagt aaaattatgg tcgctatgat
     7561 gtggtctcca tgcttcgggt cttactggaa gtactgaaca ctttcgcgtc aagttctccg
     7621 aagttcattc ctgtttgact aaactatcac tatagggacc atatctcgcc tctgacctcc
     7681 tgagagacgg caagcgagaa accgtggtcc acgctagcaa gctagccacc agcctaaggc
     7741 aagacagcct ctggtcagcc tgtgtgttct ttggtctcgt gacttaagtc tatcaccaca
     7801 cacgctcgga catgctgtcg aaccttcatc tctatggtca tcgcagacca ctaaccccaa
     7861 ttacctcaag tcattctgtt tgcgtcctgt tgcctgctgg tctcccttta cgtgggggct
     7921 tgagtaggtc agtggggtgc gacttcaggg tgcaaacaaa aggagtacac gtaaatgtcc
     7981 caacaagaaa tcagcatcat taacctcgac cagcttgtgt ccatgacctc agttgaaatt
     8041 gctgagctga caggaaaaga acataaacac gttttacgag atattcgtaa tatggtcgaa
     8101 gagcttaacg gggccaaaac tgagcactgc tcaactttga gcagcgagct taacgggtcc
     8161 aaatttgggc tcgttggcga ggaagtatac aaggacgcga aaggtgagag ccgaacaatg
     8221 tatcgtctcg accgtaagca tactttcatt ctggtcgctg gctattccgt tcacctccgc
     8281 gccaagtgtt acgaccatat tcagacgtta gagcgtcgag tccttcaact ggaagaccag
     8341 aagaagcgag cagccattca gtccgctaac cgccgaggcg tgacttgggg cgactactgc
     8401 aaaacctatg gtctgcccgc tcagaagcta atgaccgcac ttctccagca tcgtggcctc
     8461 tttcgtaaga acccaatcag taacgagtgg tctgtaaatc cgaagtattc cgactgcttt
     8521 cgtatcatta agccgtcaga ccagaagttc tcagcaggtg ggtataactt ccggttcaac
     8581 gctaaggggc tggaggtctt cggtaagcct gagatggttg acaagatgcg aggcatcctt
     8641 attgcattca ctggcaccga ccagcagaag caagagcacc tcctgaagct ggcacaatcc
     8701 ggtaaggtgg agggaatcta agatgatgat tgacccgcgc acacctgagg ggcgcatgac
     8761 tctccgttac cgtggctacc ggactgaagt cctcctcaga gagcttggtc tagacccgga
     8821 agatgagacc cgccagcacc aatcccgaga cgagctaata gcccaactgg tcgctatgaa
     8881 actgccgctt aaccgttagt agctcaccta ctcaatacct aatttaaaca ccttttgaga
//
LOCUS       DAJTYL010000141         8928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYL010000141 DAJTYL010000000
VERSION     DAJTYL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.141"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..790)
                     /locus_tag="OO572_003456"
                     /pseudo
     CDS             complement(1..790)
                     /locus_tag="OO572_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(841..1416)
                     /locus_tag="OO572_003457"
                     /pseudo
     CDS             complement(841..1416)
                     /locus_tag="OO572_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1420..1820
                     /locus_tag="OO572_003458"
                     /pseudo
     CDS             1420..1820
                     /locus_tag="OO572_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2223..2804)
                     /locus_tag="OO572_003459"
     CDS             complement(2223..2804)
                     /locus_tag="OO572_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662335.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2851..6708)
                     /gene="sigA"
                     /locus_tag="OO572_003460"
     CDS             complement(2851..6708)
                     /gene="sigA"
                     /locus_tag="OO572_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6662336.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7069..8073)
                     /locus_tag="OO572_003461"
                     /pseudo
     CDS             complement(7069..8073)
                     /locus_tag="OO572_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8058..8435
                     /locus_tag="OO572_003462"
                     /pseudo
     CDS             8058..8435
                     /locus_tag="OO572_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8474..8821)
                     /locus_tag="OO572_003463"
                     /pseudo
     CDS             complement(8474..8821)
                     /locus_tag="OO572_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2157 a         2125 c         2069 g         2577 t
ORIGIN      
        1 caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc gcccgcaggg
       61 cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac acgttcaggg
      121 ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga cgcgacagtg
      181 ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg gctcttgctt
      241 ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg atggtataca
      301 gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt gcatgcacat
      361 cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg ccggattcgt
      421 ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa ccggctaggt
      481 gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc gccgggggca
      541 tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca gttcttgttt
      601 taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact ttaccgggca
      661 tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca acggcaccgg
      721 tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat atttctgact
      781 ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg gtggagattt
      841 tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc gaccatacat
      901 ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac cattactcca
      961 gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg ccgccacgcg
     1021 ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta gctgtgattt
     1081 attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta ttttcagctc
     1141 aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt catccctggt
     1201 tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc tgacggcagt
     1261 agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga cgatggtttc
     1321 actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt tataattacc
     1381 actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac gaaaccgctg
     1441 ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga cactcagagt
     1501 tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac tatgcgaacg
     1561 atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg gtcagtctgg
     1621 gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga gcgctactgt
     1681 acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac cttcgccatg
     1741 tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt ccgtggcgca
     1801 tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta cgcttactca
     1861 tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac cctactcagt
     1921 acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca aaataaatca
     1981 accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct gaaaaacgcg
     2041 tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca acacgctcat
     2101 gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg cgtttataca
     2161 aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata ccgggacaga
     2221 gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct tactttacct
     2281 ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa tgtcacggat
     2341 gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat gagctgagat
     2401 ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat gtggcgcgta
     2461 tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag catgtgggag
     2521 acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat gataatggtc
     2581 ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa ccatgagaac
     2641 tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat agtttctttt
     2701 tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa aagaaagcta
     2761 ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc tccggtagaa
     2821 gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt tggcgttgat
     2881 cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac cgaagcgaag
     2941 attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt ctttttcacc
     3001 tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg caaacaggtc
     3061 aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg agaaggattt
     3121 acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct tcatggtcac
     3181 gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat aaaccagttc
     3241 tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg cttcagcacc
     3301 cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg tcgccgagta
     3361 ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag agtcaaatat
     3421 agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg cattactgct
     3481 ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc catccagctc
     3541 atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc caccagaagc
     3601 tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg tgtcacgcag
     3661 atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt aattaccgga
     3721 catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat atccggtcag
     3781 ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata tgttgctgaa
     3841 acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg gggctgttat
     3901 caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga ccgacagact
     3961 gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat ttacagactg
     4021 gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt ttactgtcac
     4081 agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc gggtttttaa
     4141 tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc tggctgaggc
     4201 tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca aacctgtagc
     4261 cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa caccaaacag
     4321 aatagatgct tttttgtctg attttatatt accattgacc caggcataat tttgagcctt
     4381 taacgtggca ccatcttctg tcatattata tccatctgac atatagattg ccgggctgta
     4441 tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt caatagcttt
     4501 atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa tcaggtctgt
     4561 attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa cagttatttt
     4621 gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat ccccggagcg
     4681 aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga acatgtcaat
     4741 gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt gtccttttac
     4801 attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc ggaaattaaa
     4861 tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg acttatattc
     4921 cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag aatagtcttt
     4981 ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg catgttctgt
     5041 cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt tgatatcgac
     5101 tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat tgataatatg
     5161 atttatgctt atattacctg atacattccc atggtacata tatgcatcag tattagtaag
     5221 tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat ctgttgctgc
     5281 gattttctga aatgactggt catagccgtt tagatccagt gttccgccat ttctggtaaa
     5341 atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt ttaccgtacc
     5401 tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga tgacagtccc
     5461 attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac cactacctat
     5521 tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg tattattatc
     5581 aatatctatc ccggcacctt tataagactt atcttttccg tacactttat atgtctggtt
     5641 cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg ttaatacccc
     5701 tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt gttttttacc
     5761 atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa tattattaaa
     5821 taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg tactggtaac
     5881 accatggaaa acccattttt gttgttcttt atcatataga taagaaccag aaccactatc
     5941 gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac tcttgttttt
     6001 ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt gtccaaacag
     6061 atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga agccaattat
     6121 ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg gagaaagtga
     6181 gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca gacgatgaac
     6241 cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat cccatgactg
     6301 tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt acgcacctcc
     6361 aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag gtagttctgg
     6421 aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac ccggtctgaa
     6481 ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat ttatatccag
     6541 cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa taataccgag
     6601 gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag aaacagcaac
     6661 taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac gtgaactccg
     6721 atatttctga gttgtggtta tgggatagca tttaattaca acggggaact atcagtcatg
     6781 gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta tgacagatgt
     6841 aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg atcacttggt
     6901 gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt caatccttta
     6961 caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga cttttgatga
     7021 tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc agaaggtgaa
     7081 ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg caacatcaag
     7141 gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc cgtcgttacc
     7201 ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat taaattctgt
     7261 gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg gggtcagatc
     7321 cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg tcacctgcgg
     7381 gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac tacgggagat
     7441 caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac tggcaccgat
     7501 gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg cgctgctgtc
     7561 ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt tgggggggaa
     7621 gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa tacccacatc
     7681 agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt ccacctcctg
     7741 cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga ctttaccgtc
     7801 gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat caccgggaag
     7861 ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga agtcccggag
     7921 gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga tggcgcggtc
     7981 gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat ccggattgga
     8041 gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc aaaatccacg
     8101 atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta atcggtgaac
     8161 tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg gcggtcaggc
     8221 aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag aaagaacaca
     8281 cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag cactgggctg
     8341 cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca gcagaaagag
     8401 cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat tccgtgtctg
     8461 actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca gcaagtgcaa
     8521 tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact tgtgattttt
     8581 tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct ttccatgcaa
     8641 tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat gctccaactt
     8701 ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt tcgctgtttc
     8761 caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca cctggcacca
     8821 gtctgaggtg tactggcaat agcggacact accatttgtt ctttttttaa gcagccatct
     8881 gatgatattt ttccctgaag gctgccgggg agatattccc cagacgag
//
LOCUS       DAJTYL010000142         8608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYL010000142 DAJTYL010000000
VERSION     DAJTYL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.142"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..287
                     /locus_tag="OO572_003464"
     CDS             <1..287
                     /locus_tag="OO572_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662337.1"
                     /translation="MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNF
                     YDFACYAKAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(434..2596)
                     /gene="uvrD"
                     /locus_tag="OO572_003465"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(434..2596)
                     /gene="uvrD"
                     /locus_tag="OO572_003465"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6662338.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2680..3396)
                     /gene="yigB"
                     /locus_tag="OO572_003466"
     CDS             complement(2680..3396)
                     /gene="yigB"
                     /locus_tag="OO572_003466"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6662339.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3396..4292)
                     /gene="xerC"
                     /locus_tag="OO572_003467"
     CDS             complement(3396..4292)
                     /gene="xerC"
                     /locus_tag="OO572_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6662340.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4289..4996)
                     /locus_tag="OO572_003468"
     CDS             complement(4289..4996)
                     /locus_tag="OO572_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6662341.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4993..5817)
                     /gene="dapF"
                     /locus_tag="OO572_003469"
     CDS             complement(4993..5817)
                     /gene="dapF"
                     /locus_tag="OO572_003469"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6662342.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5854..6057)
                     /locus_tag="OO572_003470"
     CDS             complement(5854..6057)
                     /locus_tag="OO572_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6662343.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6246..7724
                     /locus_tag="OO572_003471"
                     /pseudo
     CDS             6246..7724
                     /locus_tag="OO572_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7721..>8608
                     /locus_tag="OO572_003472"
     CDS             7721..>8608
                     /locus_tag="OO572_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6662344.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         1999 a         2360 c         2205 g         2044 t
ORIGIN      
        1 caatgttgat ggaaaacggt gtaattaatc cgcgtattca tcccaacgtc gcctcacgta
       61 aaattcgtaa cggtgttggg attaataaac atgggaatgc cgtgtttttg ttgagccagc
      121 aggcgacaaa tttttatgat tttgcctgtt atgccaaagc gaaactgaac gttgagcagc
      181 tgctttatct tggtggcacg atttcacata tgtatatgaa aggcggggca atcccgtggc
      241 aacgctatcc ttttgtcacc atgatttccg tcgagcggaa aggttagaat gatagctttg
      301 tgtcagatac taaatatgct gctgatgccg gatgcggcgt aaacgcctta tccggcctac
      361 atgacgttgc aatttattga atctgaatga ttttttaggc caaataaggt gcgcagcacc
      421 gcatccggca acgttacacc gactccagcc gggcgtaagc tgccaccagc catttgattc
      481 cctggccctg gaatgccacc tgcaaacggc tatgttcacc gctgccttcc atattgacga
      541 tggtgccttc accaaactta gcgtggcgta cacgctggcc gagcttgtag ccgctgtcgt
      601 tctcgaccat cggcgtaccc atccgctgat ggctgaccgg gcggcttacc gtggcgcgca
      661 ggcgcacctc ttccacacac tcttccggca actcgccaat aaagcgcgac gggcgatggt
      721 aaacctcttt gccatacaga cggcgagttt ccgcgtaggt cagcgtcagt ttctgcatcg
      781 cgcgggttac gccaacgtag gccagacggc gttcttcttc cagacgcccg ccttcatcca
      841 gcgacatctg gcttgggaac atgccctctt ccataccgac gataaacacc tgcgggaact
      901 ccagaccttt cgccgagtgc atggtcatca gttgtaccgc atcctgccag gtatccgcct
      961 gcccttcgcc tgcttccagc gccgcatggg ataggaacgc ctgcagcggc attaaatctt
     1021 cgtcttcttc gttgtagctg aactggcgcg ttgccgtcac cagttcctct aagttttcga
     1081 tacgcgtctg acctttttcg cccttctcct gctcgtacat ggtacgcagg ccggagtctt
     1141 taattacccg gtcagtctgt acatgcagcg gcatatcggc agtttcctgc gctaaggcgt
     1201 cgatcagttc cataaaccgc tgtaaggcgc tggcagcacg tccggcgagg gctttttcct
     1261 gcaacagttc acgacatgcc tgccagagtg ttaactggcg atcgcgcgat gtctgacgta
     1321 ccacgtccag cgtccggtca ccaatacccc gcgttggcgt attcaccaca cgctcaaagg
     1381 ccgcgtcgtc gttacggttg gcaatcaggc gcagatacga gagcgcatct ttgatttcct
     1441 ggcgttcgaa gaagcgcatc ccgccgtaaa tacggtacgg catactcgcc tgcaacagtg
     1501 cttcttccag cacacgcgac tgggcgttac tgcggtagag aatggcgcac tcggcaagcg
     1561 ccccgccgtt gtcctgccag gttttgatgc ggttaaccac aaaacgcgct tcatcgagtt
     1621 cgttaaaagc gcaatagagg gaaataggct caccgtccgc gccatcggtc cacagttttt
     1681 tacccagacg cccgttattg ttttcaatca gggcgttagc ggcgctcaga atattgctgg
     1741 tagagcggta gttttgctcc agacgaataa tttcggcacc ggggaaatca ttaaggaaac
     1801 gctgaatatt ctccacctgc gccccgcgcc agccgtagat tgactggtcg tcatcaccga
     1861 cgatcatcac tttgccggtg tcgcccgcca gcaggcggat ccacgcgtac tgaatgttgt
     1921 tggtatcctg gaattcgtcc accaggatat tggtaaaacg ttcgcggtag tgttgtagga
     1981 tatgcggctt gttaagccac agttcgtgag cgcgcagcag cagctcggcg aagtccacca
     2041 ggcccgcgcg atcgcacgct tcctgatacg cctgatacac cttctgccag gtctgctcca
     2101 ccggattacc gtagctttga atatgatgcg gacgcaggcc ttcatctttc tggctgttga
     2161 tgtaccacat tgcctggcgc ggcggccact gcttctcgtc gaggttcatg gctttgatca
     2221 gacgcttaag caggcgtagc tggtcttcgc tatcgaggat ctggaaatcc tgcggcagat
     2281 tggcgtccat atggtgcgca cgcagcaggc ggtgcgccag cccgtggaag gtgcctaccc
     2341 acatgccgcc ctggctggtg cccatcagtt gtccgatacg atgacgcatc tccgccgccg
     2401 ctttgttggt aaacgtcaca gccataatcg agtatggcga gcagttttcc acgctcatca
     2461 gccaggcgat acgatgcacc agtacgcgcg tcttaccact gcccgcgccc gccagcacca
     2521 gaaggttgct gcgtggcgcg gccaccgctt cgcgttgttt gtcattaagg ctgtcgagca
     2581 ggtaagaaac gtccattggc accgccgcgt aaaaataggt tgggcggaga agcgcaacgc
     2641 cctccgccaa aaagctgggt atatatacag atttgctgat tatatcagcg aggtcagaga
     2701 tgccaaccgg gaaatttcca gatgtggcag taaacggctg tcccaggttt gcatcagatc
     2761 gccattttcc ggtctgatcc aacaagcctg cattccgctg cgaattgccc cacccacgtc
     2821 agtggtgaga tcgtccccaa catgtaagat ctcgccgatc ggcacgttga gtttttccgc
     2881 agccaaaaag tacatatcgc tgaacggttt tgagcgcccg tgcgggccag cgcgcagcac
     2941 aaactcaaaa taatccccca aaccaaacag ttccggctgg gcgttaccgt tggtgatcgc
     3001 caccagcggc catttcttcg ccagctgttt taaggtgtcg tgagtttgct gcgggacgtc
     3061 gattcggctg cgccatttgg caaagttaat cattgctgcg tgtgcgcctg cactggcttc
     3121 ttcggcactc agcccggcgt cgagcatcgc ttgttcaatc gaacgaaaac gccaacgtgt
     3181 cacgtcgtga taaatctcgg gttccgcttc ccgtaccgcc tggcgcaggc gttgcagatc
     3241 ttcattctgg aagctgcgca gcgccggatg ataattttgc acaaaggtaa gcgcctctcg
     3301 ctcggtgcgc aaaatcaccg gacggttatc gtaaagggta tcatccaggt caaaggtgag
     3361 cgccgagatg cgccccaaag gccggtaaaa acgcattatt tcccccgttt ggcgcgtgga
     3421 tgcgccgcat cgtacaccga ggcaaggtgt tgaaaatcaa gatgagtata gatttgcgtg
     3481 gtggagaggt tggcatgacc cagtagctcc tgcacaccac gaagatcgcc gctcgactcc
     3541 agcatatgcg tagcgaacga gtgacgtaat ttatgtgggt gaacgtgatt attcagccct
     3601 tgttttatgc cccattcggc aaagcgtttc tgcacattac gcgcggagat acgcttgccc
     3661 agtttcgaca gaaaaagcgc gtcgtcttcg ctaccaaaca ggtcgcgcaa atcaagccag
     3721 tgctcaatcc acgccacagc gttgcgacca atcggcaggc ggcgctcttt gctgcctttc
     3781 cccatcaccc acacttcgcc ggactccagg tcgaggtgtt tgatatccag ccccaccagc
     3841 tcggaaagac gcagacccgc gccgtacatc acttccagca ttgcacggtc gcgtacagcg
     3901 aggggatcgt tgatatcaat atccagcagc cgattcatat cgtcgacgtc gatatttttc
     3961 ggcagatgac gcggcgcttt cggtgccgaa actcctttcg ccgggttagc tttgagttcg
     4021 ttctggctga ccagccagtc aaaaaagcta cgtagcgcag aaagccgtaa cgccagactt
     4081 gctgctccca gccctttacg gcgactgcgt acagcaaaat tgcgtaccat cgctgcatcg
     4141 cattgttgcc agctttgcag gccgttttcg ctggcaaaat tgatgatcgc ctcaagctga
     4201 cgctggtagt taagtagggt tatcgggcta agctgacgct ccacgctcag ataacgtagg
     4261 tagcgttcta catcggtgtg taaatcggtc atacgcgttc aatccaacgc tccagaagct
     4321 ccggcaacat cagcgcaatt tcatgaagta actgcgttcc ctgcccttgt tgatagtgac
     4381 tggcatcgcg actggtaaac agcacaacac ccaaatcagc atcgcttccc agcatcgaca
     4441 tcgccaccga tcccaccgct ttcgcttccg gtagcaccac cagcagctcc ggtccgttaa
     4501 gcggcccaag atagtgctgt tcctgcccca aacgctgaat acgcagcggt tcgaaagact
     4561 gacggcttaa tgccagatga gtgtggttcg acggcgcacc taagcgccag cgatctggaa
     4621 acaggcgcag actcgcacca gctaggccga gatcgcgcgc ccagcggtga aagcgcatca
     4681 gcatatcgtc gagactgctg gcggcggtga gactgcgctg caggtagagt aggcgataaa
     4741 acagcccttc gttggcgata gcctgctcca tcaacaacgc catgttctct tccagaacat
     4801 gaatgtgatt acgtgcgcgg gccatgtgcc actcgaccaa cgaaacggtg ccgcgtaccg
     4861 gatgcggcac acgtatcgct tctactgcgc gcgcattgcg gataaaaaac tcaggatttt
     4921 taatcagata atcgacaacc gcccggtcat caagctccgt gagtgtttcc tgcagttctt
     4981 cccctggttg cttcatagat gaataaatcc atcgtagaca tgtaccgccg ggccagtcat
     5041 atataacggg tgacccggac ctttccaggc gatatcaaga cgaccgccgg ggagttccac
     5101 gcgtacttct tcgtccagca aaccttgctg aatccctacc gccaccgccg cacacgcgcc
     5161 gctgccgcag gcctgggttt ctcctgcccc acgctcataa acgcgtaaac gaatatgctc
     5221 gcgcttaacc acttgcataa aaccgatatt ggcgcgctcc ggaaaacgct cgtgactttc
     5281 cagaacagga ccaagcgttt ctaccgccgc ggtatcgaca tcatcgacct gaatcacgca
     5341 atgcggattt cccatcgaca ccacgccgca taagattgtc tgctcggcgg cgcgcataat
     5401 ataggtcttt tccgctttgt tagcgcgaaa cggcacggcg gaaggttcga agttgggttc
     5461 gcccatattt acgcggacca gatcatcatc ggtgacggtc agaaccatcc gcccgttggc
     5521 ggtgctgacg cggatatcac gcttattggt cagtcctttc agacgcacaa aacgggcaaa
     5581 gcagcgcgca ccgttaccgc actgcgccac ttcactgcca tcagcattga aaatgcgata
     5641 gtgaaaatcc agttcaggat catacggcgg ctcaaccacc agcagctggt caaaccctac
     5701 ccccaggtgc cgatccgcca ggcgacgaat cagctccggt gaaaaaaaga cattctgcgt
     5761 taccgcgtcg acgaccataa aatcgttgcc aaggccatgc attttcgaga actgcatcat
     5821 ttactccaat cacgcgggta cagaaactga cttttagtaa ttcacctggg atggaccatc
     5881 gccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct ctaccggttt
     5941 ggtcggcggc ggtgcgtttt tatctgcagg cgggaaatag agcggacctt tcagaccgca
     6001 gcccgtcagg ctgaagagag taagtaatac agtgagtgcc ttaaacacgt ttttcattat
     6061 ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa ggcgtaaaag
     6121 caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg agtataacgc
     6181 ttactcgtga ggatttatgt tctgctggca gtttcgtgat caacacctcg cgccaggatg
     6241 gctttatgca ctatcgcaat gtggtttgtt tgttgtcttg ttcattgttt ttatcgtcgg
     6301 catggggatg tcggctttcc gcacctgaac ataatattta ccagaaacag ggaaaaggcg
     6361 tggtgtacct gcgcccttat aaagaaacaa atctttctct acctgacgtg aattacaaga
     6421 gtttgcgtcg gttacccaat ttattaatag acccgacaac actagacgaa tgggataaag
     6481 aaccgcctct gactgatttg acaaccgact acctctatga gggcgcacag gcctggtatc
     6541 ctcattattc atggcatagc gatggtcgca atatcctcta cgcgggtgaa gttgtgcaaa
     6601 accctcccgg cacacctccc gtcgatgtcg cctcgttcaa ggcatggggt gattttgccg
     6661 cagataaaca cagtctctat tttgaaggca agcgcaccga tgacaatggt ggtggaaata
     6721 gcttggatat caaaacgctg catcaggtgg aattccggcc tccctgggat cctgacttac
     6781 tggggctgat attacgtgac gctaatttct tgtatatcaa tgggcatcgc ctcgctgacc
     6841 ccgagagttt ccgtgtgctg gcgcagaagt cctgggatca gcgaggaaag ttttccacca
     6901 cgtttaaccc ctgcattgcc gtaccatttg gcccctggga taccctggct cgtacacgga
     6961 ccaaaatcct gctcaacggc gaacagcttg atgccgaccc ggacaccttt tccgtcgtgc
     7021 gctggatgcc cggctcactc ttgacctggc gtgataaaaa cgggctgcag cgtaaagtcc
     7081 tcgacaagga aaatctggcg tgggatgaag atttaacaaa gcactgtctg gatttttctc
     7141 tgctggaaaa gaaagtgttc tggcgtaaag gacctgcttg taaacaggaa gaattacccg
     7201 gactcgatcc ggagcagttt caacccatca gtgatgctgt cgcccagcat caggactcgc
     7261 tttataccat catcgaaaca gagtctggta accgtaagct ggagatcgtg aaacttgatg
     7321 atcccaatct tattatcaac aaacgtttca acgccgggaa acgccacggc tatttactta
     7381 cgcgtgccga agggtagcca taccattccg gtttacacgt gtttgaatct gacggaccgc
     7441 tgatcttact ggataaccgt tctccggatg aacgcgaagc ccatcttaat gaccatccct
     7501 ttttgcgcag atggtatgcc cgcgataacc gctacattta cagctttgat ggcgcgcagc
     7561 tctggcgata ccgcaccgct gatccgaaac aagttcgctt aatctggaag gaacaacatt
     7621 cgggatatgt ctatggcgta aattacaaaa cgggatatct ggacggaaaa attactgatg
     7681 acggcgaatt tattcctgcc ccgcgcaatg aggcgacaaa atgaagggtc aaataatcac
     7741 agttgcactc atcctgttgg gacacacatt tctgacgcct gctgtacagg ccatagggtt
     7801 cgactattac aatgatcacg gtgttatgtc ttacggtaaa ggttattggg gggataagaa
     7861 aatcattgcc cagtttccgg agatgagcag ggccggtttg cgccttgtga aaaatatctc
     7921 tgggaaaacg ggttatatac cttccgatga aataagcata gaaaatgagg aataccactg
     7981 ggtgacagac ggccgcgtta ttctctggcg tggcaaaatc gttagcaatc caccgggaac
     8041 actcactgtc gatattgcca gctttcaggc tatgggccgc ttcgcggtcg ataaatatag
     8101 cctctatttt gacggacagc gcaccgaaag taatagcggc gcgtcacgtg ttgagctggc
     8161 gacactaaaa gctatcgaag gtaactctac cacgctggtg gatagtaaaa atctctactt
     8221 gtccggtcga cgtcagggga gcagcagtga tgttactgta ttagaaaaaa gatggtgggg
     8281 tattaatcca cgtcttatga gtgtaaacag aaattcatat tccaatgatc tgcttatccg
     8341 cagtgggcag aatatttatt taaatggcgt tcaccttacg gcgaatgcag actcatttga
     8401 gataattcgc tggatacctc attcactgct ggtttttcgc gacaataagg gtctgcatcg
     8461 ttatcccttt ggtcaattat caggcaaagc gatacccgta gatgatgacg tctcttttga
     8521 agtaggggaa agtcgcgttc gctggcgtaa acagctcacg cccgaccgtc agtggagcaa
     8581 gtggatagac ctaccaggta ttgaacct
//
LOCUS       DAJTYL010000143         8602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYL010000143 DAJTYL010000000
VERSION     DAJTYL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.143"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            96..695
                     /gene="nemR"
                     /locus_tag="OO572_003473"
     CDS             96..695
                     /gene="nemR"
                     /locus_tag="OO572_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6662345.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            732..1829
                     /gene="nemA"
                     /locus_tag="OO572_003474"
     CDS             732..1829
                     /gene="nemA"
                     /locus_tag="OO572_003474"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6662346.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            1910..2317
                     /gene="gloA"
                     /locus_tag="OO572_003475"
     CDS             1910..2317
                     /gene="gloA"
                     /locus_tag="OO572_003475"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6662347.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            2420..3067
                     /gene="rnt"
                     /locus_tag="OO572_003476"
     CDS             2420..3067
                     /gene="rnt"
                     /locus_tag="OO572_003476"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6662348.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            3160..5274
                     /locus_tag="OO572_003477"
     CDS             3160..5274
                     /locus_tag="OO572_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6662349.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            5284..6576
                     /locus_tag="OO572_003478"
     CDS             5284..6576
                     /locus_tag="OO572_003478"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662350.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            7526..7618
                     /locus_tag="OO572_003479"
     CDS             7526..7618
                     /locus_tag="OO572_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6662351.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(7662..>8602)
                     /locus_tag="OO572_003480"
     CDS             complement(7662..>8602)
                     /locus_tag="OO572_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6662352.1"
                     /translation="KNFFQKRAAFPRFKKRGQNAAFRYPQDVKLDQENSRIFLPKLGW
                     MRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDAGVAKLATL
                     SDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL
                     HKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMR
                     RQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNG
                     ARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         1929 a         2323 c         2356 g         1994 t
ORIGIN      
        1 tcctcagata agattattac cattattgaa gctgttaatg tccaaagtag caactttgct
       61 tgcactagac caactggtct actacactcc aacgcatgaa caaacacacc gaacatgata
      121 ctcgcgaaca tctcttggcg acgggcgagc aactttgcct gcaacgtgga ttcaccggga
      181 tggggctaag cgaattacta aaaaccgctg aagtgccgaa agggtccttc tatcactact
      241 ttcgctctaa agaagcgttt ggcgttgcca tgcttgagcg tcattacgcc gcatatcacc
      301 agcgactggc tgagttgctg caatccggcg aaggtaacta ccgcgaccgc atactggctt
      361 attaccagca aacactgaac cagttttgcc aacatggaac catcagtggt tgcctgacag
      421 taaaactctc tgccgaagtg tgcgatctgt cagaagatat gcgcagcgcg atggataaag
      481 gtgctcgcgg cgtgatcgcc ctgctctcgc aggctctgga aaatggccgt gagaaccatt
      541 gtttaacctt ttgtggcgaa ccgctacaac aggcacaagt gctttacgca ctatggcttg
      601 gcgcgaatct gcaggccaaa atttcgcgca gtttcgagcc actggaaaac gcgctggccc
      661 atgtaaaaaa cattattgcg acgcctgccg tttagcaggc attttttatc accagacgac
      721 cgggagcctt tatgtcatct gaaaaactgt attccccact gaaagtgggc gcgatcacgg
      781 cggcaaaccg tatttttatg gcaccgctga cgcgtctgcg cagtattgaa ccgggtgaca
      841 ttcctacccc gttgatggcg gaatactatc gccaacgtgc cagtgccggt ttgattatta
      901 gtgaagccac gcaaatttct gcccaggcaa aaggatatgc aggtgcgcct ggcatccata
      961 gtccggagca aattgccgca tggaaaaaaa tcaccgctgg cgttcatgct gaaaatggtc
     1021 atatggcctt gcagctgtgg cacaccggac gcatttctca cgccagcctg caacctggcg
     1081 gtcaggcacc ggtagcgcct tcagcactta gcgcgggaac acgtacttct ctgcgcgatg
     1141 aaaatggtca ggcgatccgc gttgaaacat ccatgccgcg tgcgcttgaa ctggaagaga
     1201 ttccgggtat cgtcaatgat ttccgtcagg ccattgctaa cgcgcgtgaa gccgcttttg
     1261 atctggtaga gctccactct gctcacggtt atttgctgca tcagttcctt tccccttctt
     1321 caaaccatcg taccgatcag tacggcggca gcgtggaaaa tcgcgcacgt ttggtactgg
     1381 aagtggtcga tgccgggatt gaagaatggg gtgccgatcg cattggcatt cgcgtttcgc
     1441 caatcggtac tttccagaac acagataacg gcccgaatga agaagccgat gcactgtatc
     1501 tgattgaaca actgggtaaa cgcggcattg cttatctgca tatgtcagaa ccagattggg
     1561 cggggggtga accgtatact gatgcgttcc gcgaaaaagt acgcgcccgt ttccacggtc
     1621 cgattatcgg cgcaggtgca tacacagtag aaaaagctga aacgctgatc ggcaaagggt
     1681 taattgatgc ggtggcattt ggtcgtgact ggattgcgaa cccggatctg gtcgcccgct
     1741 tgcagctcaa agctgagctt aacccacagc gtgccgaaag tttctacggt ggcggcgcgg
     1801 aaggctatac cgattacccg acgttgtaat ccaacattgc gagcggcgta aagccgccgc
     1861 tatactaaaa caacattttg aatctgttag ccattttgag gataaaaaga tgcgtcttct
     1921 tcataccatg ctgcgcgttg gcgatttgca acgctccatc gatttttata ccaaagtgct
     1981 gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac aaatactcac tggcgtttgt
     2041 tggctacggc ccggaaaccg aagaagcggt gattgaactg acctacaact ggggcgtgga
     2101 taaatacgaa ctcggcactg cttatggtca catcgcgctt agcgtagata acgccgctga
     2161 agcgtgcgaa aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa
     2221 aggcggtact acggttatcg cgtttgtgga agatccggac ggttacaaaa ttgagttaat
     2281 cgaagagaaa gacgccggtc gcggtctagg caactaatct cctgccgggc gtgaactcat
     2341 cgcgcccgca tctttactgc atcgacaagt aatatttgtc ataatgcgcg ctgcaattta
     2401 tccgtattaa gagaatcaga tgtccgataa cgctcaactt accggtctgt gcgaccgttt
     2461 tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga
     2521 tgcgctgctt gagattgccg ccatcaccct gaaaatggat gaacaaggct ggctgatgcc
     2581 ggacaccaca ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc aaccagaagc
     2641 cctcgccttc aacggcattg acccgaacga tcccgatcgc ggcgcggtca gcgaatacga
     2701 ggcgctgcac gaaattttta aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg
     2761 cgccattatg gtggcgcaca acgccaattt tgatcacagc tttatgatgg ccgccgcaga
     2821 acgcgcctcg ctgaaacgta acccgttcca ccctttcgcc acttttgaca ctgctgcact
     2881 ggccgggctg gcactcggac aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga
     2941 cttcgacagc acccaggcgc actccgcgct gtacgacacc gaacgcactg ctgtgctgtt
     3001 ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga
     3061 ggtgtaatcg agtcgatgcc tgatgacatg caatgattca ggcatctata gtgaggctat
     3121 tccacgcatc aagcatgata ttcacgggga atagcgttaa tggcagataa tccagaccct
     3181 tcatcgctcc tgccggacgt gttttcaccg gcgacccgcg actggtttct tcgcgccttt
     3241 aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg cggcgcgagg cgaacatgcg
     3301 ctggtgattg caccgactgg ctccgggaaa acgctggcag catttctcta cgccctcgat
     3361 cggctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc
     3421 atcctctata tttcaccgat aaaagccctg ggcaccgacg ttcagcgcaa cttgcagatc
     3481 ccgttgaagg gtattgccga tgaacggcgg cggcgcggcg aaacggtaat tattgatgaa
     3541 gtccacgcgg tagcgggcag taaacgtggt gcgcatctgg cgttaagtct ggagcggctc
     3601 gatgcgctgc tccacacctc agcacagcga attggccttt ctgccactgt gcgctcagcc
     3661 agcgatgtgg cagcatttct tggtggcgat cgcccggtta cggtagtcaa cccgcccaca
     3721 atgcgccatc cgcagatacg aattgtcgta ccggtcgcca atatggatga tgtctcatcg
     3781 gtcgccagcg gcaccggcga agacagccat gccggccggg aaggctccat ctggccatat
     3841 attgaaacgg gtatccttga tgaagtgttg cgccatcgct cgaccattgt ctttactaat
     3901 tcacgcgggc tggcggaaaa actgacggca cgattaaatg agctttacgc cgcacgctta
     3961 cagcgttccc cgtctatcgc cgttgatgcg gcccatttcg agtcgacctc cggcgcaacc
     4021 tctaaccgtg tacaaagtag cgacgttttt attgcccgct cacaccacgg ctccgtctct
     4081 aaagaacaac gagcaatcac cgaacaggcg ctgaaatcgg gtgaattacg ctgcgtggtc
     4141 gcaacctcca gtcttgaact ggggattgat atgggcgcgg tggatctggt gattcaggtg
     4201 gcaacgccgc tttctgttgc cagtgggtta caacgcattg gtcgcgccgg acatcaggtt
     4261 ggcggtgtat ctaaagggct gtttttcccc cgtacccggc gtgatttagt cgattccgca
     4321 gtcattgtag agtgtatgtt cgcaggcagg ctggaaaacc tgacaccacc gcataatcct
     4381 ctcgacgtcc ttgcgcagca aaccgttgcc gccgcggcga tggatgcatt acaggtagac
     4441 gaatggtact cccgcgtacg ccgtaccgca ccgtggaaag atctgccaag acgtgttttt
     4501 gacgccacgc tggatatgct ttccgggcgc tatccctctg gcgatttttc tgcttttcgc
     4561 cccaaactgg tctggaacag ggagaccggg atattgaccg cccgacctgg cgctcaattg
     4621 ttggcggtta ccagcggcgg caccattccg gatcgtggca tgtatagcgt gttattaccc
     4681 gaaggtgaag aaaaggccgg ttcgcggcgg gtgggtgaac tggatgagga gatggtatat
     4741 gagtcgcggg tgaacgacat tatcactctc ggcgctacct catggcggat ccagcaaatc
     4801 acccgcgatc aggtgattgt gactcctgct ccgggtcgtt ctgcccagct ccccttctgg
     4861 cgtggtgaag gtaacggacg tccggctgaa ttaggcgaga tgatcggcga ttttcttcat
     4921 ttactggcgg atggcgcgtt cttttccggg actattcccc cgtggctggc agaagaaaat
     4981 acgaacgcca atattcaggg gttgattgac gagcaacgca acgcgacggg catcgttccg
     5041 gggagtcgcc atctggtcct cgaacggtgc cgtgatgaaa ttggcgactg gcgtattatt
     5101 ttgcactctc cctatggaag acgggtgcat gaaccctggg cgctggcgat tgccgggcga
     5161 atacatgccc tatggggcgc tgacgcgtct gtggtcgcca gtgatgacag cattgttgcg
     5221 cgcattcctg acactgatgg taaattgccc gacgccgcga tttttttgtt ttaaccagaa
     5281 aagttgctgc aaattgtccg cgaggcggta ggcagctcgg cacttttcgc cgcccgtttt
     5341 cgcgaatgcg ccgcgcgggc attattaatg ccgggccgca cgccgggcca tcgcaccccg
     5401 ctttggcaac aacggctgcg cgccagtcag ttgctggaaa tcgctcaggg atatccggat
     5461 tttccggtca ttctcgaaac cctacgcgaa tgtctgcaag atgtttatga tcttcccgca
     5521 ctggaacgtt tgatgcgtcg cctgaacggt ggcgaaattc aaatatccga tgtaacgacc
     5581 actacgccct cgcctttcgc cacaagttta ttgttcggct atgtcgcgga atttatgtac
     5641 cagagcgacg ccccgctggc agagcgccgg gcatccgtac tgtcgctgga cagcgagtta
     5701 ctgcgcaatc tactcggaca ggtcgatccg ggggaattac tcgacccgca ggtcattcgc
     5761 caggtggaag aagagttgca acgactggct cctggtagaa gagcgaaagg tgaagaagga
     5821 ttgttcgacc tgctgcgcga actggggcca atgaccgttg aagacctggc gcaacggcat
     5881 acaggcagca gtgaagaggt tgcgtcgtat ctggaaaatc ttcttgcagt aaaacgaatc
     5941 ttcccagcga tgattagcgg acaggagcgt cttgcctgta tggatgatgc cgccaggctg
     6001 cgtgatgccc tcggcgtacg gctaccagag tcattgccag agatttattt acatagagtc
     6061 agttacccgc ttcgcgacct ctttctgcgc tatctccggg ctcatgctct ggtcacggct
     6121 gaacaactgg ctcatgagtt tagtctcggt attgccattg tcgaagagca gcttcagcaa
     6181 ctgcgtgaac agggtctggt gatgaatctg caacaagaca tctgggtgag cgatgaagta
     6241 tttcgtcgtc tgcgtttgcg ctcgctgcaa gccgccagag aagcgacgcg tcccgttgca
     6301 gccacgacct atgcgcgatt gctgctggaa cgtcagggcg tattacccgc caccgatggt
     6361 agcccggcgc tctttgcctc aacatctcca ggcgtttatg agggcgtaga tggagtgatg
     6421 cgggtgatcg aacagcttgc cggagtcggt ttacccgcct cactctggga aagccagatc
     6481 ctgcctgccc gcgtacgcga ctattcgcca gaaatgctcg atgaattact ggcaaccgga
     6541 gcggttatct ggtcgggaca aaaaaaagct gggtgaagat gacggcctgg tggcactgca
     6601 tctacagaaa tatgccgcag aatcgttcac tcccgccgaa gcggatcagg cgaatcgttc
     6661 ggcgctgcag caagcgataa tcgctgttct ggctgacgga ggagcctggt ttgcacaaca
     6721 aatcagccaa cggatacgcg ataaaatcgt cgaatcggtt gattcctcgg cactgcaaga
     6781 ggcgttatgg gcgctggtct ggcaaggcgt cattaccagc gatatttggg caccgttacg
     6841 cgccctcacc cgcagcagtt ccaacgcacg cacctcaact cgccgcagtc accgggctcg
     6901 tcgtgggcgt cctgtctatg cacaacccgt ctcgccgcgg gtatcttaca acacaccaaa
     6961 tctggctgga cgctggtcgt tattgcaggt ggagccacta aacgataccg aaaggatgct
     7021 ggcgctggcg gaaaatatgc tcgaccgcta cggcatcatc agtcgtcagg cggtgatagc
     7081 cgaaaatatc cctggcgggt ttccatcgat gcaaacactt tgtcgaagta tggaagactc
     7141 cgggcgaatt atgcgaggtc gttttgtaga aggtctgggt ggcgcgcaat tcgctgaacg
     7201 tctgactatt gaccgattgc gcgatctggc gacacaagcc acgcaaacac gccactatac
     7261 accagtggcg ctctccgcca acgatccggc taatgtgtgg ggaaatcttc tgccctgggc
     7321 tgcacatccg gcaacgctgg taccaacgcg tcgggcgggt gcgctggtgg tcgtttctgg
     7381 cggcaaattg ttactctatc tggcgcaagg cggcaaaaaa atgctggtct ggcaggaaaa
     7441 ggaggaatta ctcgccccag agattttcca ggcgctgacc accgcactgc gtcgcgaacc
     7501 aaggctgcgc tttacgctaa cagaagtgaa tgatcttccg gtccggcaaa cgccgatgtt
     7561 tacgctgctg cgtgaggcgg gattttcaag ttcgccacaa gggctggatt ggggatagtt
     7621 gacatcctcc acgccctgaa agacgtggtt ccctgctacg ttcaagctgt cgcctgaatc
     7681 atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc acaggcaaga
     7741 acggcgtgcc ccgccgctaa aatgttacga gcgccattta catcggcgtt cgctgtatat
     7801 ccacatacct ggcatctgaa tttactttgt gacaggcggt tttctttcgc tgtatgacca
     7861 cagcacgcgc aacgctggct tgtgtacgct ggcggaacag caagcacctg accgccactc
     7921 cagagctgct tgtactcaag ctggcggcgc atttcatacc agccctgatc cagtatcgaa
     7981 cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt acccgctgct
     8041 gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg
     8101 acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt
     8161 cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt ccggcttaac
     8221 tgtcgctgaa gtctcgccag cgtcttctgg tttttttgaa aactgtttac aggctcaaag
     8281 actgtgccat ctgacagcgt ggcgagttta gccacgccag catccagccc gatcattgat
     8341 gctgaagggt gaaccggagt tgatacttca ctttctgtct gaatactgat gtaccacgta
     8401 ccgcaggact ggctgacagt gacatttttc acaactcccg tgacctgacg gctattccgg
     8461 tagcgcatcc agccaagttt cggcagaaaa atacggctgt tttcctgatc gagcttaacg
     8521 tcctgcgggt agcggaatgc agcattctgt ccccgctttt tgaatcgggg aaaagcagcc
     8581 cgcttctgga agaagttttt gt
//
LOCUS       DAJTYL010000144         8389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYL010000144 DAJTYL010000000
VERSION     DAJTYL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.144"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..300)
                     /locus_tag="OO572_003481"
     CDS             complement(<1..300)
                     /locus_tag="OO572_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6662353.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGD"
     gene            complement(367..1092)
                     /gene="fimC"
                     /locus_tag="OO572_003482"
     CDS             complement(367..1092)
                     /gene="fimC"
                     /locus_tag="OO572_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6662354.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1129..1668)
                     /locus_tag="OO572_003483"
     CDS             complement(1129..1668)
                     /locus_tag="OO572_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6662355.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1733..2281)
                     /gene="fimA"
                     /locus_tag="OO572_003484"
     CDS             complement(1733..2281)
                     /gene="fimA"
                     /locus_tag="OO572_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6662356.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(2762..3358)
                     /gene="fimE"
                     /locus_tag="OO572_003485"
     CDS             complement(2762..3358)
                     /gene="fimE"
                     /locus_tag="OO572_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6662357.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(3836..4438)
                     /gene="fimB"
                     /locus_tag="OO572_003486"
                     /pseudo
     CDS             complement(3836..4438)
                     /gene="fimB"
                     /locus_tag="OO572_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            5892..6608
                     /gene="nanC"
                     /locus_tag="OO572_003487"
     CDS             5892..6608
                     /gene="nanC"
                     /locus_tag="OO572_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6662358.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6628..7734
                     /gene="nanM"
                     /locus_tag="OO572_003488"
     CDS             6628..7734
                     /gene="nanM"
                     /locus_tag="OO572_003488"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6662359.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            7799..>8389
                     /locus_tag="OO572_003489"
     CDS             7799..>8389
                     /locus_tag="OO572_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6662360.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFN"
BASE COUNT         2473 a         1817 c         1732 g         2367 t
ORIGIN      
        1 gtcgcccgta ttaaatgtga catcacgcgt tgccatataa ccattattca aatagatatc
       61 gacgcgatac gtccctggcg gtaattcttg cccattttca aaacgcgata aatcggccac
      121 agcctgggga tcatccgcta aaaagcgcag attaaaatag aggtcggcag atgacaaagg
      181 tgcctgtgcg gcaaaagcac aggcgacaac gagtcggaca aaaaaaccag ccaaacgatg
      241 cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca gatatgacat
      301 tattcctccg gtttaaaatc acccggcagt caattctttt tattcaggcg aaaaattccc
      361 cctgcgttat tccattacgc ccgtcatttt gggggtaagt gcgccataat catttattgt
      421 tcggtaagta atattgcttc ctgcatcaga aggcaattta accgtgcttt cgcccattgg
      481 aggcaccaat gcattttcaa gaacccgggt tccggcattc aactctgtta ccgtcaggta
      541 atagggtgtc gggttaatca gcgtcagaga attcgcgcta cgacgaaatc ttaatttttc
      601 tgcggcctga tcgggtggca acgctaattt agccgggcga tagtacagtt taatgcggct
      661 gataattgcg agctgtagca tattctcagt caattttgat ttatccattg acggaatcgc
      721 tttaacgttc atccagaata aactttcccg gtcctgtggc aattggttat ttgttgcatc
      781 aagaatacgt aaggtattct ctttttttcc cttcatcgca aacagaggag gcgtcacgat
      841 aaaacgacca tcctttacac catcggcatt ttccacccat gattgaatta aataggtact
      901 attttcatca ttatttgtca cggcaagttg cacttgtttt tgccctgccg gataaattac
      961 gcgagtcgca cctaaggcca ctcctgcttc agcgcgtccg gcaaccatca ttgccatgaa
     1021 catcaggata cctgccagca agcagaatgt tatttcctgc gatttcctta cattgacgtt
     1081 tttattactc actgtcctgt tcctgttatc acattatcta ctgaacaatt actgataggt
     1141 taaagagaac caggcctggg cattggcgat gccgccagta acccgacgcc cggtagcacg
     1201 atatttggcg atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc agtttgctgg
     1261 aggacgatta atcggtacga ggtttccttc atcatcaaac aacgctacac caatattgga
     1321 ggctatccct ggcccctctc ccacggagag cacatccgga tttttaccat ccgcgacacc
     1381 gtgaaacgcc acacccacac gttcactcac caccgtgcta cattcccgta aatgaataac
     1441 aaaaggcacc ggtgcgctat cttcccctgc cgcatgaaac cggttactgc tgatttgccc
     1501 catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg caataatgac
     1561 gccctgaaat tgcatatttc cgccgggcag cgtggtattc catttatttc cggccagagc
     1621 aaacattggc agcaacgacg ctaacagaaa taatctcttc cttttcatcg ttatttttat
     1681 cgcacaaggg tgggcatccc tgcccgtaat gacgtccttg aacctgggta ggttattgat
     1741 actgaacctt gaaggttgca tccgcattag ctgcacccgg ggttgcctcg ccgattgcat
     1801 aataacgcgc ctggaacgga atggtgttgg taccgttatt cagggttgtt tgtgcactaa
     1861 atgtcgcacc gtccagcgtc agagcattgc ctgttctgtc taggatctgc acgccaacgt
     1921 ttgttgcact acctgcagca gaactctgca gagccagtac atcagtacgc gtagcatcaa
     1981 ttgccgtacc taagaaggca acagcggctt ttgtggcaac agtggtatcg caatcattca
     2041 gctgaatgtt aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg ctagcggtac
     2101 gaacctgtcc caactgaacg gtttgatcaa cagagcctgc atcaactgcg caagcggcgt
     2161 taacaacttc ccctttaaag tgaatggtcc caccatttac cgtcgtagta tcggccagag
     2221 ccgctgcgga actgagggac agagccgaca gaacaacgat tgccagagtt ttaattttca
     2281 tgctgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt ctgtacactt
     2341 tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa gatgaaacat
     2401 ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca gtttatttgt
     2461 tggcttaata ttctattatt atctttattt atagattttt atattgcatg aggtggtttt
     2521 tggagagaag aatgaggaag atgcgtcgag ccacagaaac gttagcttta catatagcgg
     2581 aggtgatgtg aaattaattt acaagagaaa taatttacat atcaaatagt tagatgcttt
     2641 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
     2701 cagtttggcc ccaattgtct tgtgtttatt tgttttttaa ctttattatc aataagttaa
     2761 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg
     2821 caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt cgatgcccga
     2881 gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa ccgcaagcat
     2941 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
     3001 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
     3061 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
     3121 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
     3181 ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca ctaatacgca
     3241 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
     3301 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
     3361 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
     3421 aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct atcagtcaat
     3481 tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta ttttcattga
     3541 tttgattcaa tataacaata gattacagag gattaacttc cagttactta aaaaataaag
     3601 aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg attttgttta
     3661 tttattttga agaacataaa tcattagatt ttgtatgggt atggttaact taatgtttat
     3721 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
     3781 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
     3841 aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
     3901 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctagataa
     3961 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatagcgt aacatgtgcg
     4021 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
     4081 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
     4141 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
     4201 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gctttaagat
     4261 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
     4321 gcaaaatcag acaataatta cgtgttgcat gaggcccggt atttgctgct ttaaggagtg
     4381 actcgatttc actatgggta aggaagttcc tttttttgtt atcagcctta ttcctcatcg
     4441 ttttccctta taattacaga cgcgcactag ctgtgctggg ttagcaataa tccaacattt
     4501 ttatcctgat tatgtttata aaagcgaacg tttgcttaat aacttacgta agtggaccag
     4561 ttcttctgag cgaacttaaa tggagtagcg atagttactt ataataactt tgccatacaa
     4621 acgccatgct cttgcatgct atgtaccttt atatatttat caatctgata cgaatctaat
     4681 aataaccctc atcattaatg caactaatct tatctatata tcatgtgata tgttctgtaa
     4741 caagtaaaat caacataaaa aattcagcaa aatttaatat ttgttaaact agccgtctca
     4801 aatatgacat tattagttaa ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta
     4861 atttcttagt ggctattatc atgctaattc ttagatccct agcgattatt cctgcctggt
     4921 tcgtcagctt aggccatgag attgttacat tcctttttat cctggagata tgggggaacg
     4981 caggcgacgc tttttcatct acatccgttg gtaaatatag ctttggtcac tacgacttaa
     5041 gccttcctca atcacagcta ttcgccgggc gatacggcaa acaaactaag tgaaacatca
     5101 tcctgaaaac accaacatca acaagcctct ccagatcgac atcaggagtg accagttaca
     5161 agccacaaac caagaaacat atttcatgat gagaattatg ctcagcctga tggcgagtaa
     5221 acccttattt tcaccactct cactgagtta gcttgcaaag ctttctttgt ttagattcta
     5281 aataaattat ttataaaacg tatagttaaa agaaaatctt tagtttttga agaccaccgc
     5341 aagtgttcgt ctggcttcac atggcatctt cttcttagaa aaagatcgac atattttgtg
     5401 acgcgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
     5461 ggtataaaga tgcaccacga tagtcaatga gactgggcat ctcgtaatct atggcaaaca
     5521 tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa
     5581 aatgagatca acagacactc tattttatga ctctgggtaa aatgggttga gtaagtgata
     5641 taacttacga atattcaaat caattaaaaa tcagaagaga ttttatactc aggtatttaa
     5701 tctggatctc tgtttattta aataatgtta aaagagaatt ttcacaggag accttataca
     5761 aaaaatataa aatacagtta cccgttgcca aagacactat aagcctggca aaaaaatatt
     5821 acacaacata aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat
     5881 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
     5941 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
     6001 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
     6061 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
     6121 atgttcaggt tgaagttaat tacgcaatta aacttgatga tcaatggacg gtgcgcccgg
     6181 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
     6241 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
     6301 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
     6361 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
     6421 agaatgatta tcgctatgca aaccacaaga aatgggcgac ggaaaatgcc tttgttctac
     6481 aataccatat gacgcccgat attacgccat acatagaata tgactacctt gaccgccagg
     6541 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
     6601 aactgtagta gacaggagac agtcacaatg aataaaacta taatggcgct tgctatcatg
     6661 atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt
     6721 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacagca
     6781 tggtacaagc tagatacaca ggccaaagat aaaaaatgga ctgcgttagc tgcattccct
     6841 ggtggaccga gagagcaagc aacctctgca tttattgatg gcaatctgta tgtgtttggc
     6901 ggcattggca aaaacagcga gggattgact caggtattta atgacgtaca caaatacaac
     6961 cccaaaacca atagctgggt taaattgatg tcgcacgcgc cgatgggcat ggcgggtcat
     7021 gtgacttttg tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc
     7081 aatggctatt ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc
     7141 aacgcccact attttgacaa aaaagcagaa gattatttct tcaataagtt tctgttgtct
     7201 tttgatccct caacacagca atggagttac gctggcgaat cgccctggta cggaacggct
     7261 ggtgcggcgg ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca
     7321 ggattgcgaa cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat
     7381 aagcttgatc ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat
     7441 gattctctta tatttgctgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac
     7501 ggtaagaact atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg
     7561 cataacggga aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca
     7621 ttgccctgga ataatagttt attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg
     7681 acggattcag ttttaatttc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc
     7741 agggccccgg taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat
     7801 gaacgcaata atatcacccg attattacta tgttcttacc gttgctggtc agtctaatgc
     7861 catggcgtat ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat
     7921 taaacaatta gcgagatttg cgcatacgca tcccggaggc ccgccatgtc actttaacga
     7981 cattattcca ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc
     8041 tctggcaacg aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg
     8101 gaaattactg cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg
     8161 cggatcggct tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca
     8221 tgatgcttgt cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg
     8281 agccgcactg gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga
     8341 atttgactta atgaccagtg actacgcgtc acaccctcaa cactttaat
//
LOCUS       DAJTYL010000145         8317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYL010000145 DAJTYL010000000
VERSION     DAJTYL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.145"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            40..735
                     /gene="minC"
                     /locus_tag="OO572_003490"
     CDS             40..735
                     /gene="minC"
                     /locus_tag="OO572_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6662361.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            759..1571
                     /gene="minD"
                     /locus_tag="OO572_003491"
     CDS             759..1571
                     /gene="minD"
                     /locus_tag="OO572_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6662362.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1575..1841
                     /gene="minE"
                     /locus_tag="OO572_003492"
     CDS             1575..1841
                     /gene="minE"
                     /locus_tag="OO572_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6662363.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2213..2557)
                     /locus_tag="OO572_003493"
                     /pseudo
     CDS             complement(2213..2557)
                     /locus_tag="OO572_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2592..2711)
                     /locus_tag="OO572_003494"
     CDS             complement(2592..2711)
                     /locus_tag="OO572_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6662364.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2672..2857)
                     /locus_tag="OO572_003495"
     CDS             complement(2672..2857)
                     /locus_tag="OO572_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662365.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            2958..3131
                     /locus_tag="OO572_003496"
     CDS             2958..3131
                     /locus_tag="OO572_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662366.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3133..3477
                     /locus_tag="OO572_003497"
     CDS             3133..3477
                     /locus_tag="OO572_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6662367.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3481..3816
                     /locus_tag="OO572_003498"
     CDS             3481..3816
                     /locus_tag="OO572_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6662368.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3873..6194)
                     /locus_tag="OO572_003499"
     CDS             complement(3873..6194)
                     /locus_tag="OO572_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6662369.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6311..6520)
                     /locus_tag="OO572_003500"
     CDS             complement(6311..6520)
                     /locus_tag="OO572_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662370.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7271..>8317)
                     /locus_tag="OO572_003501"
     CDS             complement(7271..>8317)
                     /locus_tag="OO572_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6662371.1"
                     /translation="VPLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFT
                     IIFNPPPLFSFYRLIDKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGE
                     IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQ
                     VARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTERE
                     PLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNAD
                     PESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLV
                     KIILSKPKVKVVVE"
BASE COUNT         2318 a         1950 c         1808 g         2241 t
ORIGIN      
        1 gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac tccaatcgag
       61 cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga acctaaggtt
      121 atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa acatgccccc
      181 gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat gcataaggcg
      241 gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc gcaacttaaa
      301 gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa agcgccacgt
      361 ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa aacgcgttta
      421 atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga tctgattgtt
      481 acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca tgtctatggc
      541 atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca aatattttgt
      601 acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag tgatcaaatc
      661 ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt
      721 caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt attgttgtta
      781 cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc actggtttgg
      841 cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt aatctcgacc
      901 tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt cagggcgatg
      961 caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat attcttccgg
     1021 catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg
     1081 atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg attgaaaccg
     1141 gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac ccggaagtct
     1201 cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca cgccgcgcag
     1261 aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac ccaggccgcg
     1321 taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc atcaaactcg
     1381 tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt gaaccggtca
     1441 ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg
     1501 gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc aaacgcttgt
     1561 tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag aacacagcca
     1621 acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc gatgcagaac
     1681 cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa
     1741 ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt tctattcttg
     1801 agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt aaaaacgcat
     1861 ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat ccacgttgtg
     1921 gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc cccctttaca
     1981 ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt aaataaccat
     2041 gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt cattgttctc
     2101 ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga attttctctt
     2161 tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat ggtcagaacg
     2221 accagttcac accagccacc gcgttccacg gggactccac accggcacca tggctatacc
     2281 ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     2341 ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact ttcacccgat
     2401 tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt tccataccgt
     2461 tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata ctgcgggtat
     2521 cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt gacctaagct
     2581 ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag agacagcgca
     2641 ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata tcccgcataa
     2701 gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa atcactcttt
     2761 tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa aataaaatga
     2821 ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac aataatattg
     2881 ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca cttcttgaat
     2941 aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt cacgtaaaaa
     3001 acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat agcgagcgtt
     3061 ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact tacttaatac
     3121 cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc attgttctgc
     3181 tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct aggcgcaggc
     3241 gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc tgttggcgct
     3301 ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa
     3361 gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct cgaaggggcg
     3421 attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat gaaataagga
     3481 atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc aatgacctgg
     3541 gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa
     3601 gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc ggtaatgggt
     3661 aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact gagcgccaaa
     3721 gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt gatcacggca
     3781 attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg
     3841 aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag gcccaccatc
     3901 actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc ccagacgctg
     3961 gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt actcacccca
     4021 tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt atggatccag
     4081 ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta
     4141 cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc attctcaata
     4201 cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc
     4261 tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt attcagggca
     4321 aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga ttcataggct
     4381 aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga gtcaatgtat
     4441 gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc gctatagcct
     4501 ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt gtagtcgcgg
     4561 gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc accggttaac
     4621 agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg
     4681 tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga acgctcctgt
     4741 tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg cataacaaaa
     4801 agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg caggacgggg
     4861 gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc gctcgttgcc
     4921 tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg ggttaatgaa
     4981 tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt tgattccccc
     5041 tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc tgcattggta
     5101 actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc cgtagctgaa
     5161 ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac gtaattttca
     5221 gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat atagaccgta
     5281 ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca ggtactatta
     5341 tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc tgaacccgtt
     5401 atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat aaaaacatct
     5461 gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc tttatggtag
     5521 aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt tgcttcctga
     5581 ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc agatgtattt
     5641 ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg acccgttgta
     5701 taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc atgagcattg
     5761 tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt ataatcaagc
     5821 gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc tgttccgtgg
     5881 gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc attacttcct
     5941 gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct tccgggagat
     6001 gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt atcatctccg
     6061 tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt gtcagtattc
     6121 aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt gacaatcgtg
     6181 tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct agctgttaat
     6241 gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac aatgacgcca
     6301 ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt ccattacccg
     6361 ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt gccacagcat
     6421 gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc gccagagcaa
     6481 taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta tccattcatt
     6541 ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa acggagggta
     6601 aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata tataaatgat
     6661 aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac tttggggtga
     6721 ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt
     6781 atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat
     6841 aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg
     6901 acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat
     6961 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     7021 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     7081 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attattcatg
     7141 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     7201 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     7261 tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca
     7321 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     7381 ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     7441 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     7501 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     7561 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     7621 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     7681 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     7741 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     7801 gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa
     7861 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc
     7921 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     7981 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     8041 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgctgg
     8101 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     8161 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     8221 tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata atgtcatccg
     8281 ccacgcgtaa tacatacctt acccctttca agggtac
//
LOCUS       DAJTYL010000146         8283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYL010000146 DAJTYL010000000
VERSION     DAJTYL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.146"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            827..1045
                     /locus_tag="OO572_003502"
     CDS             827..1045
                     /locus_tag="OO572_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6662372.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1263..1505
                     /locus_tag="OO572_003503"
     CDS             1263..1505
                     /locus_tag="OO572_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662373.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1835..2764)
                     /gene="fdhE"
                     /locus_tag="OO572_003504"
     CDS             complement(1835..2764)
                     /gene="fdhE"
                     /locus_tag="OO572_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6662374.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2761..3396)
                     /gene="fdoI"
                     /locus_tag="OO572_003505"
     CDS             complement(2761..3396)
                     /gene="fdoI"
                     /locus_tag="OO572_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6662375.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3393..4295)
                     /gene="fdoH"
                     /locus_tag="OO572_003506"
     CDS             complement(3393..4295)
                     /gene="fdoH"
                     /locus_tag="OO572_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6662376.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4308..7358)
                     /gene="fdnG"
                     /locus_tag="OO572_003507"
     CDS             complement(4308..7358)
                     /gene="fdnG"
                     /locus_tag="OO572_003507"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6771..6773),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6662377.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7552..>8283
                     /gene="fdhD"
                     /locus_tag="OO572_003508"
     CDS             7552..>8283
                     /gene="fdhD"
                     /locus_tag="OO572_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6662378.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATT"
BASE COUNT         1954 a         2104 c         2183 g         2042 t
ORIGIN      
        1 ttttgatatt cgagttcttc atgtctctaa caaattcaat gttggattgc aaggtaatat
       61 caacactgat gttttacaag attcgttttt taaaaatgtc gatttcattc atgaatataa
      121 ctcgtatacc gtagggatgg gagtctgacg agtaaaactc attaggatta tcagaattcg
      181 gacaatgcat tgcaaaaatg acatacaaag agagatgtgt gcaggtactt agccgcgagg
      241 ggcatggcga agccatacag aaagtaccag tattcagaac tccagtgaaa gcggcatagt
      301 ggccgcctgc tcaggagttt tgctatgacc cgtaatcgtg ctgaacgtcg ccaccctatg
      361 atgcgattaa aaaaactcat cgtcgcgctg atgattgacg tcaatgctat cggatgatgc
      421 cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga
      481 tgagcaagta aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc
      541 accttaaatc ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg
      601 taaataaata tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa
      661 ctaaaccatt tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac
      721 gcctcgcaaa aacaaaatga catgcaaaaa tccgcatttt tttaaatgct tttccggcgt
      781 aattttcgca catacattaa tgagtaatat atgtaaggtg gtggtaatgg cgatgaatac
      841 ggtttttctt catttatcag aagaggcaat taaacggctg aacaagcttc ggggctggcg
      901 taaggtttca cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca
      961 atttccggtg cgtaaagcaa agggaggcag gcaaaagggg gaggtagttg gtgttgatga
     1021 tcagtgtaag gagcataagg aatgaaaaaa atttttcatt cttgaatata aaaaacagat
     1081 gcccttattc tggtattaat acaaggctgt tttacttgaa cttataataa ctgcaactgt
     1141 tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt
     1201 taaagattcg gcaagggtac ttttctctcc atagattcaa aaatgatatt ggcgagatat
     1261 ttatgaactc tctggcaggt atagatatgg gcagaatttt actcgattta tcgaatgagg
     1321 tgattaagca acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa
     1381 gggaagcggt agatcaatac ctgataaatc aatcgcaaac agcaagaacc agtgttcctg
     1441 gcatctggca agggtgtgag gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat
     1501 ggtaatggga aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg
     1561 atgcggcgtg aacgccttat ccggcctaca aagtcatgca aattcaatat attgaagttt
     1621 attggtaggc ctgataagcg cagcgcatca ggcaatcata cgcttgtcat cagtgacata
     1681 cccataacct tatcgatgtg ttggagggaa gaacgaggca aacagacaat tgcggcatga
     1741 tcttgaatgg gtaacgcgga atacgagaga tggtgtcgtc acaccctatg agctgtaatc
     1801 cgggcgacct gcgccacccg gaaaacgtca gagattactc tccttcaccc ggaaacagga
     1861 acgggttaat ggaactacgg gcgtagcctt cttgctccat tcgcgcgtcc agtaccagag
     1921 aggcgaggtc atctgccacg gcttcaactt tcggttcttt ttcctgataa agaatcttca
     1981 ggtaagtgcc gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg
     2041 accagtaatg cagtttgccg ctctgctcgc agttgctgca ttttacgcgc actacgtgcc
     2101 attcggtttc acacaggttg cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca
     2161 ccatgctgga caccggcata gagccacata ccgggcaata ttgacgttgt tcgccgtatt
     2221 cagcgcgggc tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagcgcag
     2281 cccagataaa cggcgcttta tcgctgctga cggacgagaa atcagaggca aacagcgcgc
     2341 tggccatatc ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg
     2401 ccgggccgct catttcaggt ttcagctcag caattagcgc catcagcagc ttttgccagt
     2461 gcttatcacg cggcagaacg tgaatatcca gcgggggctt gccctgtgcg ctggcttctt
     2521 taatgcgcgc ggtcagatcc atctccagcg gatggtcgta cagcaccact ttctgggcgt
     2581 gggcgataag cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc
     2641 gcagacgctc ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa
     2701 tcatatccgc cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac
     2761 tcattcagct ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt
     2821 cttcgcccag gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca
     2881 aagggcggcg tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg
     2941 cagcattaac gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca
     3001 gataatcacg ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca
     3061 tttctgaccg aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg
     3121 aatattcttc gcccaaaaga tatcatcccg attgattagg ttttgatgcc agtaacggaa
     3181 aaacatgatg atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc
     3241 cagctgcggt gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag
     3301 cccgctcacc gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg
     3361 cgcggtgtag cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg
     3421 tgcagattat tctcttcctc atccgcacgg ttcggaccga cacccacgta gtggaagata
     3481 ctggccgcaa aggtagccgc aaagccaaca gccgcgagcg gtttccagat gcctttccag
     3541 aatttcacgg tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg
     3601 tcagcatggt gcagcacgta catgacgtgt gtaccaccga cgcctgccgg atcgtacaga
     3661 cccgcattgt cgtaaccgcg ggttttcagc tcagcaacgc gctcgctcgc cagcgttttc
     3721 atcgactctt tcgtgccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt
     3781 tcttgcccaa ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc
     3841 gggttgaggc gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac
     3901 tgctcggact ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg
     3961 cacgctttca ggcagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat
     4021 tccagtttgt cgttctgctc cacttccgag aagcgcatta ccgtccacga tttggcgctt
     4081 aaatcattgg ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc
     4141 cactctgaac acgccacctg acaggcttta cagccgatac aggtggtaac gtcgatgagt
     4201 ttcgccactt cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg
     4261 gaacgacgaa tgatatcttg agattgataa gccatatgtc gtctccgtta caccttttcc
     4321 acattcacaa ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc
     4381 gttaaagtat tggcaataaa gcctttttta gctacgcctt catagcccca gtgaatcgga
     4441 ataccgatgg tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc
     4501 accgctttgg ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca
     4561 atgccaagtt tattcgccag cgactccccg atttccacaa actgctctgg ctgcaaaatc
     4621 gcgttcaaca gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt
     4681 ccgacatacg ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt
     4741 gcagctgggt tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac
     4801 ggctcgtagt gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc
     4861 atgccttcct gctgcatgat aaacggcccg acgccgctac ccggaggcgc tgcgctgtag
     4921 tccggaatat cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga
     4981 tcccacgggt taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc
     5041 ggccatgccc atgcccagcc cagcgtgtta ccgaggccag acggatcggc gttatcacga
     5101 cgcgccatct ggttgccttc cggcgtccag ctaccggcga aaatccagca gccacaggag
     5161 gttgtaccgt catcgcgcaa ttgggcgaac gagctaagtt gttggccttt cttgacgata
     5221 accgcaccgg ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgct
     5281 acttcttccg acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctga
     5341 tccgggttcg cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag
     5401 aggatctcac cgtcagtcag cgcaatcccc ggggcgtccg cacctttcca gtgccactgc
     5461 aaccagcgac ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga
     5521 cggaacactt cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc
     5581 cagaagttag aggtttcagt gttcagtggg tcgatggtga cgaggaactt cagtttcgac
     5641 agacaaccga tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag
     5701 atatagccat tgaccttgcc ctctttcatc atctcgaagt attgcaggac gtcgtagcct
     5761 ttatcccact tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc tttatcacca
     5821 aagaaggcct tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttacctgg
     5881 ccttccagca gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc
     5941 tcgcttggca gcgtcatgta acctggcaga ctctgcgaca gtagccccag gtccgtcagc
     6001 ccctgaatat tggagtgacc gcgcagggcg ttaacgccgc cgccggccat ccccatattg
     6061 ccgagcagca gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc
     6121 gtccagccga gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg
     6181 tattcgcaga ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct
     6241 ggcgtgtagc gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc
     6301 gtggtatcgc gtttggcgaa gccgttttcg tccagttcat aagtccagga ggatttatcg
     6361 tacttgcgtt tttccgcgtc gtagccggtg aacaggccat cttcaaagcc gtaatcctca
     6421 cgcacgatca ggctggcgtt ggtataggct tcggtgtatt cgtggttgaa tttttcattg
     6481 ttcagcaggt acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg
     6541 gcatagtagt cagctaccgc cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc
     6601 gcgccgttgt gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg
     6661 gcgttaccgc ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc
     6721 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc
     6781 gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg
     6841 gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc
     6901 acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg
     6961 atgcgatcaa acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg
     7021 tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc
     7081 ggacaaagtg caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat
     7141 gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa
     7201 caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc
     7261 gcgagtgcta cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca
     7321 gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat
     7381 tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt
     7441 gcggaagggg ccgcaaaggt ccagaattgg ctcaatttcc ctccatctag gaagggtttg
     7501 taacagaata ccataatgtt ggtgtgtatg ttcttatctg gttaagagaa agtgaaaaaa
     7561 acacagcgaa aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta
     7621 tggcggcgtg atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt
     7681 gcgctggtct acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag
     7741 tactttgcgc tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc
     7801 ggcatggatg tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc
     7861 cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta
     7921 tgcggcgtgg agcaacttaa tgacatcgga aaaccggtgc agccgctacc gttcacccag
     7981 acgtttgatc tcaacaaact ggatgatgca ttacgtcatc tcaacgattt tcagccagtg
     8041 gggcgactga ctggttgtac tcacgccgct gcctggatgt tgccatctgg cgaactggtc
     8101 ggcgggcatg aagacgtggg tcgccatgtg gcgctggaca aactgttagg ccgccggtca
     8161 caagaagggg aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa
     8221 atggtgcaaa agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc
     8281 acg
//
LOCUS       DAJTYL010000147         8051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYL010000147 DAJTYL010000000
VERSION     DAJTYL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.147"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..212
                     /locus_tag="OO572_003509"
     CDS             <1..212
                     /locus_tag="OO572_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662379.1"
                     /translation="DAILFQEGVSVKLRLPQISESGEVVDMAAYEAWQQENHPDTWQQ
                     MQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(221..619)
                     /gene="vapC"
                     /locus_tag="OO572_003510"
     CDS             complement(221..619)
                     /gene="vapC"
                     /locus_tag="OO572_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6662380.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(619..846)
                     /gene="vapB"
                     /locus_tag="OO572_003511"
     CDS             complement(619..846)
                     /gene="vapB"
                     /locus_tag="OO572_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6662381.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            928..1299
                     /locus_tag="OO572_003512"
     CDS             928..1299
                     /locus_tag="OO572_003512"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6662382.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1320..6181
                     /gene="traI"
                     /locus_tag="OO572_003513"
                     /pseudo
     CDS             1320..6181
                     /gene="traI"
                     /locus_tag="OO572_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6201..6947
                     /gene="traX"
                     /locus_tag="OO572_003514"
     CDS             6201..6947
                     /gene="traX"
                     /locus_tag="OO572_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6662383.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7002..7562
                     /locus_tag="OO572_003515"
     CDS             7002..7562
                     /locus_tag="OO572_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6662384.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7693..7905
                     /locus_tag="OO572_003516"
     CDS             7693..7905
                     /locus_tag="OO572_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6662385.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1831 a         2043 c         2592 g         1585 t
ORIGIN      
        1 ctgatgccat tctatttcag gaaggagtgt ccgttaaact caggctacct cagatcagtg
       61 aatccggtga agtggtggat atggccgctt atgaggcatg gcagcaggaa aatcatccgg
      121 acacctggca gcagatgcag cgtcgtgaag aggtgaacat caatgtgcac cgggaacgcg
      181 gggaggatgt tgagccggga gatgatttct gatgaacagg tcagctccag tcttcagttc
      241 tcaggccgcc cacacgttca aattcccggg tgttattagt cacaataatc agcccccgac
      301 tgcgggcatg acctgcgatc atttgatcaa atggcccgac agggcgtccc tgacgggcaa
      361 gttctgctct tatctggccg gtgtgtgtgg ctgcagcagc gtcgtaatcc agaacatcaa
      421 tgcgggaaac aaatccctcg atcacagcga gattacgttc aggcatctgg cttttttctg
      481 caccatatat cagctccatc agggtgaccg aactgataca cattttcccc tggttcaggt
      541 taaaacgctc cctgacgctg gcgggtttgt tctttatcgt aaaaatgcag atgttggtat
      601 cgagcataaa cttcagcatc agaatgactc cctttcttgc atgccgggct gttccctgtt
      661 atccataaaa tcggtgctga cgctgtgtcc gtcgaaccat tcgtcccatg tctctccggc
      721 tggcgtaatg attctggttc gtccgacagc aatcacttca acgcgtttta cgttttccgg
      781 caatgcaacc gcttttggca gtctgaccgc ctggctgcgg ttgctgagaa atacggtggt
      841 ttccattatt tgcctcctta tgtggatatg tcttgtgtat atctattgtg ggattgatac
      901 cgggatatgt caaagggata tacgtttatg atgagtatcg cgcaggtcag atcggccgga
      961 agtgccggta attattatac cgacaaggat aattactatg tgctgggcag catgggagaa
     1021 cgctgggccg gccagggggc tgaacaactg gggctgcagg gcagtgtcga taaggatgtc
     1081 tttacccgcc ttctggaggg caggctgccg gacggggcgg atttaagccg catgcaggat
     1141 ggcagtaaca ggcatcgtcc cggctacgac ctgaccttct ccgctcccaa aagtatctcc
     1201 atgatggcca tgctcggggg ggacaaacgt cttattgaag cacataacca ggccgtggat
     1261 tttgctgtcc gtcaggtgga ggccagtgcc tccacctgac ggacggacag tcagaaacgg
     1321 tgctgaccgg taatctggtg atggcactgt ttaaccacga caccagtcgc gatcaggaac
     1381 cacagttaca cacgcatgcg gtggtgacta atgttacgca gtataacggc gaatggaaga
     1441 cactgagcag tgacaaagtg gggaaaacgg ggttcattga gaatgtgtac gctaatcaga
     1501 ttgcctttgg caggctctac cgggaaaaac tgaaagagca ggttgaggcg ctgggctatg
     1561 aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc gggtgtaccg gtggaggcct
     1621 tttccggacg ctcacagact atccgggagg ccgtcgggga agacgcctcg ctgaaatccc
     1681 gggatgtggc ggcgctggat acgcgtaaat ccaaacagca cgtcgatcct gaagtcagaa
     1741 tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga catccgggca tatcgtgacg
     1801 cagcggaaca acgggcatat acccgcacgc agacacccgg acccgcttca caggacgggc
     1861 cggatgtgca gcaggcggtg acacaggcca ttgccggatt aagtgaacgc aaagtgcagt
     1921 ttatgtatac ggacctgctg gccaggacgg tcggcatact gccgccggaa aatggtgtga
     1981 ttgaacgggc acgcgccggt atcgatgagg ccatcagccg tgagcagctt atccccctcg
     2041 atcgtgagaa ggggctgttc acgttcggga ttcatatgct cgatgagctg tcagtccggg
     2101 cactcagtcg tgacatcatg aaacagaacc gggtgaccgt acatccggag aaaagtgtcc
     2161 cccggacggc cggttacagc gatgccgtca gtgttctggc acaggaccgt ccgtcgctgg
     2221 ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga gcgggtggct gaactggtca
     2281 tgatggcccg ggagcagggg cgggaggtgc agattatcgc tgctgaccgt cgctcgcaga
     2341 tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat aaccggacgt cgtcagttgc
     2401 tggaaggaat ggccttcacg ccgggcagta ccgttatcgt cgaccagggc gaaaaactct
     2461 ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg tcataacgta caggtcctga
     2521 taaccgacag cgggcagcga accggtacag gcagtgcact gatggccatg aaggatgccg
     2581 gggtgaacac atatcgctgg caggggggag aacagcgacc ggccaccatc atcagtgaac
     2641 cggaccgtaa tgtcggctgg ccggagattt tgcggccagc gtgaaagccg gagaagagag
     2701 cgtggcacag gtcagcggag tacgggaaca ggccatactg acacaggcca ttcgcagtga
     2761 gctgaaaaca cagggcgtgc tcggacaccc ggaggtaacc atgaccgccc tgtcaccggt
     2821 ctggctggac agccggagcc gttatctgcg ggatatgtac cgcccgggaa tggtgatgga
     2881 gcagtggaac ccggagacac gcagtcatga ccgctatgtg acagaacgtg tgacggcgca
     2941 gagtcacagc ctgaccctgc ggaatgcgca gggcgaaacg caggtggtgc gtatttcttc
     3001 cctggacagc agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag acggtgagcg
     3061 tctgagggtg acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc gcctgcaggt
     3121 ggcatccgtc agtgaagatg cgatgacggt tgttgtgccg ggacgggctg aaccggccac
     3181 cctgcctgtg agcgattcac cgttcactgc actgaagctg gagaacggct gggtggaaac
     3241 gcccgggcat tccgtcagcg acagtgcgac ggtttttgcc tccgtcacac agatggcaat
     3301 ggacaatgcc accctgaatg gtctggcccg cagtggtcgt gatgtccggc tgtattcctc
     3361 actggatgaa acccgtactg cggaaaaact tgcccgccat ccctccttta cggtggtttc
     3421 tgagcagata aaggcgcgtg ccggtgagac attgctggaa accgctatca gtctgcagaa
     3481 agccgggctt cacacgccgg cacagcaggc cattcatctg gcccttcctg tgctggaaag
     3541 taaaaacctg gccttcagca tggtggacct gctgacagag gcgaagtcgt ttgctgcaga
     3601 aggaaccggt tttgctgacc tgggagggga aatcaatgcg cagataaaac ggggtgattt
     3661 actgtatgtg gatgtggcaa aaggctatgg cacaggcctg ctggtttccc gtgcgtcgta
     3721 tgaggcagaa aagagcattc ttcgccatat tctcgaaggt aaggaggcgg tcacgccgct
     3781 gatggagaga gtacctggcg aactcatgga gaaactgaca tcaggacagc gcgccgccac
     3841 ccgtatgata ctggaaacgt ctgaccgttt cacggtggtg cagggctatg ccggtgtggg
     3901 taagaccaca cagttccggg cggtgatgtc agccgtgaac atgctgccgg agagtgaacg
     3961 tccccgtgtc gtggggctgg ggcccacaca ccgtgcggtc ggggagatgc gcagcgccgg
     4021 cgtggatgca cagacactgg cgtcctttct gcatgacacg cagctgcagc agcgcagcgg
     4081 agaaactccg gatttcagca acacgctgtt ccttctcgat gagagctcta tggtgggcaa
     4141 taccgacatg gcacgggcat acgccctgat tgcggccggt ggcggtcggg ctgtggccag
     4201 cggtgacacg gaccagctgc aggccatcgc gcccggtcag cctttccgtc tccagcagac
     4261 gcgcagtgct gccgatgtgg tcatcatgaa ggagattgtg cgtcagacgc cggaactgcg
     4321 ggaggcggta tacagcctga ttaaccggga tgtggaaagg gcgctgtccg ggcttgagcg
     4381 tgtgaaaccg tctcaggtgc cacgtctgga gggcgcatgg gcaccggagc actccgtgac
     4441 ggagttcagt cacagccagg aagcgaaact ggcagaagcg cagcagaagg cgatgctgaa
     4501 aggcgaggct tttccggatg tccccatgac actgtatgaa gccattgtcc gcgactatac
     4561 cggcaggaca ccggaagcac gggagcagac gctgattgtc acgcacctga atgaggaccg
     4621 gcgcgtactg aacagcatga ttcatgatgc acgggaaaag gccggagaac tggggaaggt
     4681 gcaggtcatg gtgcctgtcc tgaacacagc gaatatacgc gacggggagc tgcgtcgtct
     4741 ctccacctgg gagaataacc cggatgccct tgccctggtg gataatgtgt atcaccggat
     4801 tgccggtatc agtaaggatg acgggctgat aaccctgcag gatgcggaag gtaacacgcg
     4861 gctgatttcg ccccgggagg cggtggctga aggcgtcacg ctgtacaccc cggacaccat
     4921 ccgggtgggg accggtgaca ggatacgctt cacgaagagt gaccgggagc gcggttatgt
     4981 ggccaacagc gtctggacgg tgacagcggt ttccggtgac agtgtcacac tttctgacgg
     5041 tcagcagacc cgtgtgattc gccccggtca ggagcgggca gagcaacata ttgacctggc
     5101 ctatgccatc accgcccacg gtgcacaggg ggcgagtgaa acctttgcca tcgcgcttga
     5161 aggcacggaa ggtaaccgga aactgatggc cggctttgag tcagcctacg tggccctgtc
     5221 gcgtatgaag cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat
     5281 caacaatgcc gtacagaaag gaactgccca cgatgtgttt gagccgaaac cggaccggga
     5341 ggtcatgaat gcagagcggc tgttcagtac ggcgcgggag ctgcgggacg tggcggcagg
     5401 ccgtgccgtt ctccgtcagg cggggctggc cgggggagac agtcctgcac ggtttattgc
     5461 tccgggacgt aaatatccgc aaccgtatgt ggcactgccg gcgtttgacc gtaacggcaa
     5521 gtccgccggt atctggctga acccgctgac cacggatgac ggaaacgggc tgcggggatt
     5581 cagcggtgaa ggtcgggtaa aaggcagcgg ggatgcgcag ttcgtggccc tgcagggcag
     5641 ccgtaacgga gaaagcctgc tggctgataa tatgcaggat ggcgtccgga ttgcccgtga
     5701 taatcctgac agtggtgtgg tggtaagaat cgccggtgaa ggccgtccgt ggaatcccgg
     5761 tgccatcacc ggtggccgcg tgtgggggga tatcccggac aacagcgtcc agccgggagc
     5821 cggaaatggt gaaccggtca cggcagaggt actggcacag cggcaggctg aagaggccat
     5881 ccgccgtgaa acggaacgtc gcgcagatga aattgtccgt aaaatggcag agaacaaacc
     5941 tgacctgccg gacggcaaaa cagagcaggc tgtcagggaa attgccgggc aggagcgtga
     6001 ccgggctgcc ataactgaac gggaagccgc gctgccggag agtgtactgc gtgaaccaca
     6061 acgggtgcgg gaagcggtcc gggaagttgc ccgggaaaat ttgctgcagg agcgtctgca
     6121 gcagatggag cgggatatgg ttcgtgacct gcagaaagag aaaaccccgg gcggagactg
     6181 atacaggaag acaaacactg atgacaacgg ataacacgaa cacgacacgt aacgattcac
     6241 tggctgcacg gaccgatacc tggttgcagt catttctggt ctggtcaccc ggacagcggg
     6301 atatcatcaa aacggtggca ctggtgctga tggtactgga ccacatcaat ctgatattcc
     6361 agctgaagca ggaatggatg tttctggccg ggcgaggagc ctttccgctt tttgccctgg
     6421 tgtgggggct gaatctgtcc cgtcatgcgc atatccggca accggccatt aaccggctgt
     6481 ggggatgggg aattattgcc cagttcgcat attatctggc aggctttcct tggtatgagg
     6541 ggaatatcct gtttgccttt gcagtggcgg cacaggtgct gacgtggtgt gaaacgcgca
     6601 gtgggtggcg tacagcagcg gccatactcc tgatggccct gtgggggcct ttgtccggca
     6661 ccagttacgg tattgccggg ttgctgatgc tggcagtcag caaccggctg taccgggcgg
     6721 aagacagagc ggaacgtctg gcgctggtcg cctgcctgct ggctgttatt ccggcgctta
     6781 accttgccac cagtgatgca gcggcggtgg ccggtcttgt gatgacggtg ctgaccgttg
     6841 gtctggtgtt gtgtgccgga aaatcattac cccgtttctg gcccggagac tttttcccga
     6901 cgttctatgc ctgtcatctg gctgtgctgg gcgttctggc gctgtgacgg gtgtggtatc
     6961 tttggccgca agaggatgat tcgtcagagg cagaacacag catgacagag cagaagcgac
     7021 cggtactgac actgaagcgg aagacagagg gaacagcgcc tgtccgcagc cggaaaacca
     7081 tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa gcagaagctg gccgagaaag
     7141 cggcccgtga agcagagctg gcggcaaaaa aagcgcaggc cagacaggcg ctgtccattt
     7201 atctgaacct gcccacgctg gatgaggctg tgaataccct gaagccctgg tggccgggat
     7261 tatttgacgg tgacacgccc cggcttctgg cctgcggtat ccgggacgtg ttactggaag
     7321 acgtggcgca gcggaatatc ccgctctcgc ataaaaaact gcgcagggcg ctgaaggcca
     7381 tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg tgcctgccgg tatgacacgg
     7441 aagggtatgt gacggagcat atttctcagg aggaggaagc gtatgcggga gcgcgtctgg
     7501 cgaaaattcg tcgccagaac cggataaagg cagaacttca ggccgtgctt gatgagaaat
     7561 aaaaaaagcc tcccccagag agaggaggca gggaaataag gcttaaaagg aaataacttc
     7621 cgcttccgaa aggaagccag tccactgaat tatttatcac aataatactg acaactaaag
     7681 ggggacaaga aaatgaatgg attcagaaac agttcacgga acggccaggt ctggcgttac
     7741 cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg gacgctggat ggaagcggca
     7801 gaagcatggc gacgggctgc ctgtgttgcg cctaggacag actggcagca gtttgcccgg
     7861 aaaagggctg aacactgcca ccggcgctgc cggggaaggg tgtaacgaaa aaagcagcct
     7921 tcattactct aggagtcaga aggctgaaag gagtttatga gatttgcttc ttaatatccg
     7981 gtcgggcagt atgaccggac agttctgagt agcacagagt cttttccgca ttaagtcgat
     8041 tttttatatt t
//
LOCUS       DAJTYL010000148         8037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYL010000148 DAJTYL010000000
VERSION     DAJTYL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.148"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1407)
                     /gene="tap"
                     /locus_tag="OO572_003517"
     CDS             complement(<1..1407)
                     /gene="tap"
                     /locus_tag="OO572_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6662386.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQV"
     gene            complement(1453..3114)
                     /gene="tar"
                     /locus_tag="OO572_003518"
     CDS             complement(1453..3114)
                     /gene="tar"
                     /locus_tag="OO572_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6662387.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(3259..3762)
                     /gene="cheW"
                     /locus_tag="OO572_003519"
     CDS             complement(3259..3762)
                     /gene="cheW"
                     /locus_tag="OO572_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6662388.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(3783..5747)
                     /gene="cheA"
                     /locus_tag="OO572_003520"
     CDS             complement(3783..5747)
                     /gene="cheA"
                     /locus_tag="OO572_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6662389.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(5752..6678)
                     /gene="motB"
                     /locus_tag="OO572_003521"
     CDS             complement(5752..6678)
                     /gene="motB"
                     /locus_tag="OO572_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6662390.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(6675..7562)
                     /gene="motA"
                     /locus_tag="OO572_003522"
     CDS             complement(6675..7562)
                     /gene="motA"
                     /locus_tag="OO572_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6662391.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(7689..>8037)
                     /locus_tag="OO572_003523"
     CDS             complement(7689..>8037)
                     /locus_tag="OO572_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="With FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator FlhC"
                     /protein_id="HCR6662392.1"
                     /translation="NAWQFLLKTGLCNGVDAVIKAYRLYLEQCPQAEEGPLLALTRAW
                     TLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPSRAVKRRKLSQNPADI
                     IPQLLDEQRVQAV"
BASE COUNT         1814 a         2229 c         1994 g         2000 t
ORIGIN      
        1 aacctgctca atcccccgcc gttgttcttc cgacgccgaa gcaatttctc ccataatgtc
       61 gttcacgcgg gtcaccgaac tgacaatatc ggtcatggtc gcggcggcgt tattcaccag
      121 tttcgaaccc tgctggacac gattgactga ctcttcgatc agccctttga tctcttttgc
      181 tgcctgcgcg ctacggctgg caagattgcg cacttcacct gccactaccg caaaaccacg
      241 cccctgctct ccggcgcgag ccgcttccac tgccgcattc agggccagaa tattggtctg
      301 gaaagcaatt ccgtcgataa cgctgataat gtcgccaatt ttttgcgagc tggtggcgat
      361 ctcctgcatg gtgtgagtca tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc
      421 ggcatttttt gccagttccg acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc
      481 ggttaattgc tccatactgg cggccgtttg tgccagcgat gccgcctgct gttcggtacg
      541 acttgagaga tcgttattgc ctgcgacaat ctccgcgatc ccaatgtgca tctcatggct
      601 tcccttacgc acatcactca ccgtcccacg caaagcctgc tgcatggtct tcaggctggc
      661 aaaaatggcg gtgatctcat tacgaccata taccgcaatc ggacgcgcca gattacccgc
      721 agcaatgctg tcaaaatggc taccgataat ggccagtggt tgaacaatca tcttgcgcgt
      781 ccaccacagc gcactgctga tgtagatcgc cgcaacaata atcatgctga taaacaccag
      841 cgccgaaatc tgatagttac gctggctttg cgcactggcg gcttccagca catggttgat
      901 ctccagctgc caggcctcaa agttaacgtc aaacgcattc tgtgatccct gcaccggcgc
      961 agtgaggaaa tccgaaagtt gattactttc aagccaggtc gcctgatgtt cgagatcgtt
     1021 gtgccagcgg gcaaaacttt tctccgtctc tttttgcaat gccctgacgt gctcgttgcc
     1081 cgcagtcatc gccataaaac ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc
     1141 cgtcgtcatc aacgttttaa tgtcatccgc cggatagcta agcgctgtca gagtgcctgc
     1201 tttgttcagc gcggtactgg cctgtaacat taccgcccga gtttgcgcta atgccgcacg
     1261 ttgctgattg ctctgctcgc cgtgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa
     1321 agacatgccg ttactgccaa tctgcaagat cccgcagaga atcaaaatta aaaacagcgt
     1381 ggtcgaaatt cgaatacgat taaacatcaa cgctccccat caggcggcaa tgaccgcgtt
     1441 agtaaaaact cgtcaaaatg tttcccagtt tggatcttgt tcagtaattc gcagtcgtgg
     1501 ctgtgccggt ggttgctcac tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg
     1561 gctggctgcc agacggaacg cggaaaccgc ttgcgttaaa cgactcgcct gttcttccag
     1621 cgcagctgcg gcggcagctg attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc
     1681 catttccgaa accgccaatg cgacttgatc gatgccacgg ctctgttcat ccgatgccga
     1741 tgcaatctcg cccataatgt cagtcacgcg agtgacagca ttgacgatat tgttcattgt
     1801 ttccccggcg ctttcgacca gcaccgaacc ggtatcaatg cgcgagacgg agtcttcaat
     1861 gagggctttg atctcttttg ccgccagggc gctgcgactg gcaagattac gcacttcacc
     1921 cgccaccacg gcaaaaccac ggccctgttc acccgcacgc gcggcttcaa ccgcggcatt
     1981 cagcgcgagg atattagtct ggaaggcaat accgtcgata acgctgataa tgtcggcaat
     2041 tttcttcgaa ctatcggcga tctcatgcat cgttttcact acgccatcca ccactttgcc
     2101 gccgtgctgg gcggtgtcgg aggcactttg cgccagttgc gaggcctggc gggcgttatc
     2161 ggcgttttgc ttcactgtcg cggtgagctg ctccatgctg gcggcagttt cttccagcgc
     2221 ggatgcctgc tgttcagtac gggaggaaag atcggtgttg cccgccgcaa tttcacgggt
     2281 accggcatag atggcatctg aaccttcgcg gacatgagtg acggtgtcag tcaaagagcg
     2341 ttgcatatgt gaaacgctct gcgccaggtc acccatttca ctgcgcccgt caatggtcag
     2401 ggtattcgcc aggttaccac cggcgatttc gcaaatgtga gcaataattt ttgccagcgg
     2461 agtaagcaac atacggcgaa tgccgtacca cgccaccagc agaatcaata ccaccaccag
     2521 cgcgataacc gccagttgcc actgggcaaa tcggtaatca tctgcgttgt cagtgacgat
     2581 atcgcgatac agtttttcac tgctgagggc gtactgagca aacgcttcgc ccattgcatt
     2641 ttgcattccc tgggttggct gagcgaaata agctccagta ttgccataat caagataatc
     2701 aatcagttca gttaacgctg tgtaatagtt tttatatttt tcatcaaaat tacggctggt
     2761 agcgaccatt tcaggtaacg gtgccatgct tttgaatttt ttataatgcg tcgcggcctg
     2821 ggccaatgtt ttcctggcgc tatcgagcaa ttcaactttg gcgttacttt gttgattgga
     2881 ggaatccatc atcatccgca ccgctgaacg actcaggtta atgcgcgttt gcagcattaa
     2941 atcccaggtt gacgtcagct cgccctgctg ttcccgtaat tgattggaaa ccacaaagct
     3001 cttctggcta tggtgaaggg aagaaaaaaa caaactgccg gaaataagct gtaacagtgc
     3061 gaataccccc agcaccatta ccaacagcgt gactacgcgg atacggttaa tcataaggca
     3121 ccttcctgaa aacaagttga tctcgttatc ggcaagaagg ggggaaactt tattgctgat
     3181 gccacccgcc gcgaaattga aataaaaaac ccgatgcgca gatcatcggg ttcatttcaa
     3241 ttgaggaaat cgggagaatt acgccacttc tgacgccgcg ctatctaaca gcgccatctc
     3301 ttcgctgttc agcagttttt cgatgttcac cagaatcaac atacggtcgc ccagtgcgcc
     3361 cagtccagtg agatattctg ttgaaagcgt cacggcaaac tctggtgccg gacgaatttg
     3421 ctccgccgtc aatgaaagca cgtctgaaac gccgtcaacc acgatgccga ccacccgctg
     3481 tccgagattc aggacgataa ctaccgtgtt gtcgttatag tccacatcca cctggctgaa
     3541 cttaattcgt aagtcaacaa tcggcacaat aacgccgcgc agattcgtga cgcctttgat
     3601 aaacgctggc gtgttcgcaa tccgtgttac ctgatcgtag ccacggatct cctgcacttt
     3661 caggatatca ataccgtact cttcatcacc aagggtaaat accagaaatt cctggcctga
     3721 cggctcgctg gccagctttg ttacattcgt cataccggtc atattgttac ctttttactc
     3781 attcaggcgg cggtgttcgc catacgttgt tcgcggttta tcgcctgcaa ggcggagaca
     3841 tcaacaatca gtgccacgct gccgtcgcca agaatggtcg cagcagaaat gccggggact
     3901 ttgcgatagt tactttcaag gtttttaacc acaacctggt gttgaccaat taattgatcc
     3961 accagcaagg cgtagcggcg accgccactt tgtaagatca ccacaattcc ctgggtggct
     4021 tcggttttcg cgcccgcgac gttgaacact ttccacagtt cgacgatggg cagatattca
     4081 ccccgcactt ccagcacccg ctcgccgccg gccagtggat ggagatcggc ttcacggggt
     4141 tgcagtgatt ccataacagc attcagcggc agaatgaaaa cttcatccgc aacgcgtacg
     4201 gacatgccgt cgaggatggc cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta
     4261 ccctgcttcg actggatttc gacatgaccg cccatctcct ggatattacg tttaacgacg
     4321 tccatgccga cgccgcgccc ggagacgtcg gtgacctgct ctgccgtgga gaagcctggt
     4381 gcaaatatca gcattgcaac ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc
     4441 gaggccgctt ttgccagaat tcgctcacgg tttagccccg ccccatcgtc ggtcacttca
     4501 atgcaaatgt tgccgccctg atgttcggca gacagaatta aatttccgac gctgttttta
     4561 cctgcggcga gccgtttttc tggcagttca ataccgtgat cgaggctatt gcgtaccagg
     4621 tgggtcagcg ggtcgataat gcgttctatc aggctcttgt cgagctcggt ggaactgccc
     4681 accagcgtca gttctacctg cttgccgagt tttcccgcca gatcacgcac cagccgggga
     4741 tagcgactaa agacatattc catcggcatc atgcgaatcg acatcactga ttcctgcaaa
     4801 tcacgggcgt tacgttgtaa ctgccccatg ctggtaatca aatcaccatg attaaccggg
     4861 tccagttcgc tggaacgctg ggcaagcatg gactgggtga taaccagctc gccgacgagg
     4921 ttaattaatt gatcaacctt ttctaccgct acacggatgc tggtggattc actgctgcgc
     4981 gtcgtttttt cccgctccac gcggccggtt ggcgcttgtt cggctgccag tttaagcaca
     5041 ggtggggtag ctatttttgg cgaaacttct actgtttcaa aggtaatctg atcggcttca
     5101 atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tatcgcccgg taatattgcc
     5161 gagagagaat ccgccccttt caccacgtca tttaacgttg tcagatgccc cagctcttct
     5221 tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg cggcaactga
     5281 ctgcgacttt gctcatcttg cggttcactt ttggcaacca cacttaatcg ggtcactgcg
     5341 gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca gatataatcg
     5401 tagctggcgg catccggctc ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg
     5461 tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc acctcgtctg
     5521 gcttcatcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct gaagccaaaa
     5581 gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa ttgttcggca
     5641 tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tatcagccaa cagttcgtcc
     5701 gcttcatcaa aaaatgtctg ataaaaatcg cttatatcga tgctcacgct gtcacctcgg
     5761 ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt tttctagggc
     5821 gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt
     5881 gttcagtacc aacaggctga tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa
     5941 gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgctatcca accctccgac
     6001 catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt tgctgtaacc
     6061 tttctcaccg ctggcgtagg gaaaatcatc ggtatgacct gaaaggctaa tacggttggg
     6121 aataccgttc agcacaggcg cgatggcgcg cagaatgtcg cgcatatagg gttcgacatc
     6181 ggcactgccg gttctaaaca tcgggcgatt ctggctatcg atgatctgaa tacgtagacc
     6241 ttcctggacc agatcgattt tgagatggag acgtaacgcc cgcagtttcg gatcggactc
     6301 tatcaactgg tcgagatcac cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt
     6361 cagctcttcg atgttcggct gcttattcac ttccccctgg ctttgggtgt aatcatcacc
     6421 accgccggga attgggcttt cactattgga aatgcgatcg ccgcccgtaa ccgctgtcgc
     6481 cagtggagtc cggaagtact ccgcaatctg aatcaactct tttgggctgg agatggagat
     6541 cagccacatc accagaaaaa aggccatcat cgcagtcata aagtcggcat aagcaatctt
     6601 ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt cgtttgacga caataatcgg
     6661 atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca
     6721 cgcacatgct cttccagttc aataaacgac ggacgttcgc tggaatagag cgttttgcga
     6781 ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag agtgactttg
     6841 acgcactgca tcattttgct ggtttcggcg cttttctgac gtaaaacact cgctagtggg
     6901 gaaataaatc cgtaagccaa taaaataccg aggaaagtcc ccaccatcgc atgtgcgata
     6961 agcgccccca gctcggcggc aggacgatcg gctgaaccta acgcgtgaac gccccccatt
     7021 acagccgcga caataccaaa cgccggaagt gagtccccga ccagcgccag actgttcgcc
     7081 gggacttctg cctcgctttc gtgcgtctca atctcttcat ccatcagggc ttcgatttcg
     7141 aaggtgttca tgtgaccgct gataatcagg cgcagataat cgacgataaa atcaagcatg
     7201 acgctatccg cgaggatgcg tgggtagctg gcgaagatct cgctctcacg gggattttca
     7261 atatcacgtt ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa ccgataaagc
     7321 agagccagca gatccatata cattgctttg gtgtatttgg agcgacgaaa cagcaacggc
     7381 agcgctttca gcgtgccttt aatcgctttg ccattattgc cgacgataaa cgacccaatc
     7441 cctgcaccgg caataatcac cagttcagcg ggttgataga gtgctccaag gcttccaccg
     7501 gtcatcaaat aaccgccgaa aactgtaccg agaacaacca ggtaacctaa taagataagc
     7561 acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg cgttaacgcg
     7621 cgattgggga aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt tgcgccacac
     7681 cgtatcagtt aaacagcctg tactctctgt tcatccagca gttgtgggat aatatcggca
     7741 ggattctggg aaagtttacg tctttttact gcccgggatg gcggttgaca taagctgcag
     7801 gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca gcagttgcag
     7861 ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc acgggttaat
     7921 gccagcagtg gtccttcttc tgcttgtggg cactgttcaa ggtataaacg gtaggctttg
     7981 atcaccgcat cgacgccatt acacaaaccg gttttcagta aaaactgcca tgcatta
//
LOCUS       DAJTYL010000149         7982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYL010000149 DAJTYL010000000
VERSION     DAJTYL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.149"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..213)
                     /locus_tag="OO572_003524"
     CDS             complement(<1..213)
                     /locus_tag="OO572_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR6662393.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIAL"
     gene            complement(232..2109)
                     /gene="bglF"
                     /locus_tag="OO572_003525"
     CDS             complement(232..2109)
                     /gene="bglF"
                     /locus_tag="OO572_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6662394.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2243..3064)
                     /gene="bglG"
                     /locus_tag="OO572_003526"
     CDS             complement(2243..3064)
                     /gene="bglG"
                     /locus_tag="OO572_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6662395.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(3351..4076)
                     /gene="phoU"
                     /locus_tag="OO572_003527"
     CDS             complement(3351..4076)
                     /gene="phoU"
                     /locus_tag="OO572_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6662396.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4091..4864)
                     /gene="pstB"
                     /locus_tag="OO572_003528"
     CDS             complement(4091..4864)
                     /gene="pstB"
                     /locus_tag="OO572_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6662397.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(4956..5846)
                     /gene="pstA"
                     /locus_tag="OO572_003529"
     CDS             complement(4956..5846)
                     /gene="pstA"
                     /locus_tag="OO572_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6662398.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5846..6805)
                     /gene="pstC"
                     /locus_tag="OO572_003530"
     CDS             complement(5846..6805)
                     /gene="pstC"
                     /locus_tag="OO572_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6662399.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(6892..7932)
                     /gene="pstS"
                     /locus_tag="OO572_003531"
     CDS             complement(6892..7932)
                     /gene="pstS"
                     /locus_tag="OO572_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6662400.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT         2011 a         2145 c         1895 g         1931 t
ORIGIN      
        1 taacgcgata tcttccgggt aacggtaata aaaatcgatg gcgacatctt tgatattctc
       61 tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta aatctgaggt
      121 cgtgatccct ttgccatctt ccagccaggc accttccacc tgattggcag ctgttgcgcc
      181 accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat
      241 gatggataac agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt
      301 aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat
      361 agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac ccacgttgac
      421 gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg ataccgacat gaatcaggat
      481 ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat
      541 tcgacccgca accggagaac gcacttcacc aaccgagggc agaatggcga tacctttacc
      601 caacaggcca ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt
      661 catcggtgaa cagataccgc cctgctcagg tggtgtaata acctctggtg ttttctcttg
      721 cggggcaccc tgcgctggct gacgtttact ggtgatgaaa tgaagcatca acgtaccaac
      781 aaatgcgcaa cctatggcaa tgacaccgcc aataacgctg gcccagacgg tgaaatcaat
      841 ccccgttgac gggatggtct gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac
      901 tttcgtttgc gcgtagccaa tgatggtggc ccccaatgct ccactgatac aggcgataac
      961 aaaggggtac ttacgcggca ggttgacgcc atataccgct ggttcggtga taccaaacag
     1021 acccgtcaac gccgctgatc ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa
     1081 gacgccgagc gccgccccga cctgcgccat aatggcgggc attaacagcg ggatcatggt
     1141 gtcgtagccc agcacggtga agttattgat acacagcggc accaggcccc tgtgcagtcc
     1201 gaacatgacg aagatttgcc agaagccgcc cattaccgcg cccgcaaatg caggaaccgc
     1261 ctgataaagc cagagataac cggcggcaat catttcgctt atccaggttg atagctgccc
     1321 caccagcaga aaggtgacgg gtgtgataac catcagacat agcaatggtg tgaagaaatt
     1381 tttgattgcc gacggtaacc acgcattaag tcggcgttcc agaatgctgc acaaccaggc
     1441 agaaaaaata atgggaataa ccgatgacga gtaattcaac aatgtgaccg gaatacccag
     1501 gaaatccagc cccggcgcat ccgctttttg cccgttctcg aaggcagtca gaattaatgg
     1561 atgcactaac gctccaccaa tcaccatggc agtaaatgga ttaccgccga agcgtttgcc
     1621 cgcggtgtat cccaggatta tcgggaagaa cccaaacaag gcatcactgg cgctgaataa
     1681 aattaaataa gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat
     1741 acctttcagg atcccggttg ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat
     1801 aacataaaca aagcggttta gcagattacc tttctcatca ttttccggtg cctgttgcgc
     1861 tttttcgcca aggcctgcca cgctgttaac cgccaggaag acatcggcca catggttacc
     1921 tatgaccacc tgaaactgac caccgctttc caccaccata ataataccgg gggtcttttt
     1981 cagtacctca gcttgcgctt tgctttcatc ctttaactta aaacgtaatc gcgttgcgca
     2041 atgcatcaga ctcacaatgt tatctgcgcc cccgactcct gcgactattt ttctggctaa
     2101 ctccgtcata acttgccctc taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa
     2161 aacggcctga cgtgaatcaa gcaatttttt tcaggttttg cccgcttagt gcggtaacaa
     2221 tcctttactc agtaataata tttcagtgtt ctttgcgcac gcgctctata tttatggcta
     2281 aaaacataat ctctgcgggt gaaattttac gttgatactg caaaccaata aaaatggcga
     2341 tccgttccgc acattgccat gcttgcgggt aattttgttt tactgcttgt tgtaatgatt
     2401 catcactatc gttaattgaa gcatgttcaa gaatacgcca ggataaaaac ttcagatgtg
     2461 taaccagtcg ctgataactc aagctttcct cctggtaatt aaggctgaac tgaaatttta
     2521 ttaattgcag catttcgcgc attaactgcg tgacacctgc aacatcctcc atatttccgc
     2581 tcatttgggc actgaccaga tgcatggcaa taaagcccac ttcatctttc ggtaactgca
     2641 cgcccaaccg tttatcaata atggttaacg cttcttcccc tagctggaac tctttcgggt
     2701 aaagccgctg gatatcccac agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc
     2761 aatggtcagt tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta
     2821 aagagataag acgatcacag gttgccatca cctcaagagg aatatgactt aagagttcgc
     2881 ttaatcgccc gttcagttca tgactgctca aggcatactc tttttctatt ccacttgagt
     2941 taattctttc gccagcgcgt ttttgaaagc caattccgcg ccccatgacg actttttccc
     3001 gctgttgatc atcaataacc accacaacat tattgttgag aattttggtg atttgcatgt
     3061 tcatagcaag gacctttttt ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt
     3121 atgtcaggtt ttgcctgcga atgcagtaac aatccagtca tttattaatg gtttttataa
     3181 cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt
     3241 gataaaaata tgaccatgct cgcagttatt aactttgtgt aattttagga atttataaag
     3301 ttatatataa caaatcccaa taattaagtt attgggattt gtctggtgaa ttatttgtcg
     3361 ctatcttttc ccgccagcag tttatccagc tcatcaccgc ctacatgacg gaaatcctga
     3421 cccttcacgt agtagaagat aaactcacaa atattctggc agcggtcgcc gatacgttcg
     3481 attgaacgcg cacagaacag cgcggtaagt acgctcggaa tagtgcgcga atcttccatc
     3541 atgtaggtca tcagttgacg aacaatacct tcgtattcct gatcgacttt tttatcttca
     3601 cgataaatac gtaccgcttc gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc
     3661 agcatctgga tggtatgacg gcccagcgac tccagactta ccagcaacgg ctgatgctgc
     3721 tgggagaatt tctccagcgc agtacggcag attttgtccg ccacgtcgcc aatacgctcc
     3781 agctcggcaa tggttttact gatcaccata accagtcgca ggtcgctcgc cgtcggctga
     3841 cgtttggcga taatgcgcac gcaggcttca tcgatcgcca cttccatcat gttgacgttc
     3901 ttgtcgcctt cgatcacgcg cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt
     3961 gcatcagaaa gctgctgctc caccatgccg cccatggtca tcacctgcgt gcggatactt
     4021 tccagttcgg cgttgaactg gccggaaata tgtttattaa gattgagact gtccataacg
     4081 cactcctgaa tcaaccgtaa cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct
     4141 tggtgaacag atcgtccgtg ttgctgaact caatcaattc gcccaggtac ataaatgccg
     4201 tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt gacgatcacc acggtgtaat
     4261 cctgcttcag ttcggtgatc agctcttcaa tacgcccggt agagatgggg tcgagcgccg
     4321 aacacggttc gtcgagcagc agcacttccg ggcgaatggc gataccacgc gcaatacaca
     4381 gacgctgttg ctgaccacca gagagagagt aaccgctctg gtgcaattta tctttggttt
     4441 cgttccacaa tgcggctttg gtcaatgccc actgcacgcg ctcgtccatg tcggcacggg
     4501 agagcttctc aaacagacga acgccaaagg cgatgttgtc gtagatggac atcgggaacg
     4561 gcgtcggttt ctggaacacc atgcccactt tcgcacgcag cagggcgata tcctgagagt
     4621 tggtcaggat gttgtcgcca tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca
     4681 gttcaaacat tttgttgaag gtacgcagca gcgtcgattt accgcagccg gacggcccga
     4741 taaacgccgt aacctggttt ttggcgatat ccaggttgat gtttttcagg gcatggaatt
     4801 tgccgtagta gaagttcaaa ttacgaacct gaattttact cggggcagtt tcaaccatac
     4861 tcattgcaat cttttcctca tcgtacctga tgcttttcgc taatcaggct taccgtaggg
     4921 ccgggtaagg cgttcacgcc gcatccggaa aagtgttaac cgtgtttatt cttcgcaaaa
     4981 acaacgcgcg ccagaatgtt cagcagcagg acgcacaggg taatgatcaa taccccggcc
     5041 caggccagtt gttgccattc cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc
     5101 aggttggcga tcggctgcat catgtccgtg ctccagaact ggttggagag cgcggtaaac
     5161 agcagcggcg cggtttcacc ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc
     5221 ccggacaccg acgctttcag cgtaatcgcg gagatcatct tccacttcgg tgtacccagc
     5281 gcataagccg cttcacgcag gctgtccggc accagtttca gcatgttctc agtggtgcgg
     5341 ataacgatcg gcacctgcaa cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc
     5401 tccatctgtg ccaccacgat ggtgtagaca aacagaccaa ccacaatcga cggcgcggag
     5461 agcagaatgt cgttaatgaa gcgaatcact tctgccagcc aggatttacg accatattcc
     5521 gccagataaa tccccgccat aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt
     5581 aacagcccgc tacccgccag ggcgttcgcc agaccaccac cttccgtatt gggcggcggc
     5641 gtcatttcag tgaacagcgc cagcgacata ccgtcgatac cgcgagtgat ggtggacatt
     5701 aaaatccaga tcagccagaa caggccgaag gccatcgtcg ccatcgagag cgtcagcgca
     5761 atacggtttt tgaggcggcg acgcgcctgc attttgcggc gagattcagc cagcgccgca
     5821 gtggtttgca tttcaaccat agccattagc gtgccccctc attcttagcc aggcgcataa
     5881 tcataaactt cgatgcggcg aggacgatga aggtaatcac aaacaggatc aggcccagtt
     5941 ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc aaattcgttc gccagcgcag
     6001 aggtgatact attgcccggc atgtacagcg aggcgctgtc gagctggtag gtgttaccga
     6061 tgataaaggt caccgccatg gtttcaccga gcgcgcggcc cagccccagc atgatgccgc
     6121 cgataacacc atttttggtg aacggaagaa cgatacgcca gataacttcc caggtggtgc
     6181 agccaatacc gtaggccgac tctttcatca tcaccggggt ttgttcgaac acatcacgca
     6241 ttaccgccgc aatgtacgga ataatcatga tggcgaggat cacgcctgcc gcgaggatac
     6301 cgataccaaa tgcggggcca gagaacagcg cgccaacaat cgggatattc gacatgatat
     6361 tgccgaccgg ctcctgaaag taaacggcga acagcggcgc aaagataaac aggccccaca
     6421 tgccgtaaac gatacttgga atggctgcca gcagctcaat ggcgataccc agcgggcgtt
     6481 tcagccagcc aggcgcaagc tcagtcagga acagggcgat accgaaactc accgggacgg
     6541 cgatcagcag cgcgataaac gaagtcacca acgtaccgta gatcggcacc agcgccccgt
     6601 agatatcgtt cggtgcatcc cactctttgg tccataggaa agccagacca aatttctgaa
     6661 tgctcggcca ggaggagatg atcagagaga caataatgcc acccaacatc aatagcacaa
     6721 tcagcgccgc cagttttacc agcacgctga aaattatgtc gcccttttta cccggtgggt
     6781 taaaagcggg cttggttgca gccataagtt actcttcagt taaacgcgtt tacaaagtca
     6841 ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc ctggagtttt attagtacag
     6901 cggcttaccg ctactgtctt taatattggt cttccacgca gcgcgaacct gttcaactac
     6961 actatccggc aggctggcgt aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc
     7021 ccagtcgaag aatttcagca cctctgtgcc ttgttctggt ttcttctgat ctttgtggat
     7081 cagaatgaac gtggtagagg taataggcca tgcatcttcg cctttctggt tggtcagatc
     7141 ctgagcgaag gttttgctcc agtctgcacc ttttgctgca ttagcgaagt tttcttcggt
     7201 cggactaacc ggtttaccat cagcggagat cagtttggtg tacgccaggt tgttctgctt
     7261 cgcgtaagca tattcaacat aaccaattgc acccggcaga cgctgaacga acgcggcgat
     7321 accgtcgtta cctttaccgc ccagaccgat cggccatttt acggtagagc cagtaccaac
     7381 gttgtttttc cactcttcgt tcactttcgc caggtagctg gtgaagacga aggaagtccc
     7441 ggagccatct gcgcggcgta ctacagcaat gttttgtgaa ggcagtttca gacccggatt
     7501 cagtttggcg atggcttcat catcccactt cttgattttg cccaggtaga tgtcgccgag
     7561 ggttttacca tccagcacca gttcgccaga cttcagccct ggaatgttaa ccgccagcac
     7621 cacgccgcca atcacggtcg ggaactggaa cagaccttcc tgagccagtt tttcgtcaga
     7681 cagcggcgcg tcagaggcac caaaatcaac ggtattagcg ataatctgtt ttacgccacc
     7741 ggaagaaccg ataccctggt agttaacttt attaccggtt tctttctggt aagtgtcagc
     7801 ccatttggca tacaccggcg cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc
     7861 aaacacagag aaagcactca tcgataaggt cgcggcgaca acagttgcga cggtggtacg
     7921 cataactttc ataatgtctc ctgggaggat tcataaagca ttgtttgttg gctacgagaa
     7981 gc
//
LOCUS       DAJTYL010000150         7933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYL010000150 DAJTYL010000000
VERSION     DAJTYL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.150"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1774
                     /gene="ldtD"
                     /locus_tag="OO572_003532"
     CDS             <1..1774
                     /gene="ldtD"
                     /locus_tag="OO572_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6662401.1"
                     /translation="FNAQADEPEVIPGDSPVAVSEQGEALPQAQATAIMAGIQPLPEG
                     AAEKARTQIESQLPAGYKPVYLNQLQLLYAARDMQPMWENRDAVKAFQQQLAEVAIAG
                     FQPQFNKWVELLTDPGVNGMARDVVLSDAMMGYLHFIANIPIKGTRWLYSSKPYALST
                     PPLSVINQWQLALDKGQLPTFVAGLAPQHPQYAAMHESLLVLLSDTKPWPQLTGKATL
                     RPGQWSNDVPALREILQRTGMLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDK
                     QTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVL
                     ALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNGNQVLDSRVIVGRPDRKTPMMSSALN
                     NVVVNPPWNVPPTLARKDILPKVRNDPGYLESHGYTVMRGWNSREAIDPWEVDWSTIT
                     ASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNK
                     ASDLANMLLQDAGWNDKRISDALKQGDTRYVNIRQSIPVNLYYLTAFVGADDRTQYRT
                     DIYNYDLPARSSSQIVSKAEQLIR"
     gene            1955..2503
                     /locus_tag="OO572_003533"
     CDS             1955..2503
                     /locus_tag="OO572_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6662402.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2530..3177
                     /gene="gloC"
                     /locus_tag="OO572_003534"
     CDS             2530..3177
                     /gene="gloC"
                     /locus_tag="OO572_003534"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6662403.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3400..4590)
                     /gene="aspC"
                     /locus_tag="OO572_003535"
     CDS             complement(3400..4590)
                     /gene="aspC"
                     /locus_tag="OO572_003535"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6662404.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4775..5863)
                     /gene="ompF"
                     /locus_tag="OO572_003536"
     CDS             complement(4775..5863)
                     /gene="ompF"
                     /locus_tag="OO572_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6662405.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6464..7864)
                     /gene="asnS"
                     /locus_tag="OO572_003537"
     CDS             complement(6464..7864)
                     /gene="asnS"
                     /locus_tag="OO572_003537"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6662406.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2038 a         1955 c         1989 g         1951 t
ORIGIN      
        1 gttcaatgcg caggccgatg agcctgaagt aatccctggc gacagcccgg tggctgtcag
       61 tgaacagggc gaggcactgc cgcaggcgca agccacggca ataatggcgg ggatccagcc
      121 attgcctgaa ggtgcggcag aaaaagcccg cacgcaaatc gaatctcaat tacccgcagg
      181 ttacaagccg gtttatctta accagcttca actgttgtat gccgcacgcg atatgcaacc
      241 catgtgggaa aaccgtgatg ctgttaaagc cttccagcaa cagctggcag aggtggcgat
      301 tgctggtttc cagccgcagt ttaataaatg ggtagagtta ctgaccgatc ctggtgttaa
      361 cgggatggca cgtgacgtgg tgctctctga tgcgatgatg ggctatctcc atttcattgc
      421 aaatattccg atcaaaggca ctcgctggct atatagcagt aaaccttatg cgctttcaac
      481 gccgccgctt tcggtgatta accaatggca gctagcgctg gataaaggtc aattgcctac
      541 gtttgttgca ggactggcac cgcagcatcc gcaatatgcg gcgatgcatg aatcgttact
      601 ggtcttactc agtgacacca aaccgtggcc ccaactgacc ggcaaagcaa cgttgcgccc
      661 agggcagtgg agtaacgacg taccggcgtt gcgcgaaata ttgcaacgca caggcatgtt
      721 ggacgggggg ccgaaaatta ctctacctgg cgatgacacg ccaactgacg cggtagtcag
      781 cccatccgct gttactgttg aaacagcaga aactaagccg atggacaagc aaacgacgtc
      841 tcgtagtaaa cctgcgcctg ccgttcgtgc cgcctacgat aatgaactgg tggaagccgt
      901 taaacgtttt caggcatggc aaggattggg agcagatggt gctattggcc cggcaacgcg
      961 tgactggtta aacgtaacgc ccgcccagcg tgctggcgtg ttggcactca acatccagcg
     1021 attgcgcttg ctgccaacag agctttctac cgggatcatg gttaacattc cagcctattc
     1081 gctgctctac tatcagaacg gcaatcaggt gctggattcg cgagtcattg tcggtcgccc
     1141 cgatcgcaaa acgccgatga tgagcagtgc acttaacaac gtagtggtaa acccgccgtg
     1201 gaacgtacca ccaactctgg cacgcaaaga tattctgccg aaagtgcgca acgatccggg
     1261 atatctcgaa agccatggct atacggtgat gcgcggctgg aacagcagag aagcgattga
     1321 cccatgggag gttgactggt ctacaatcac ggcctcgaat ttaccgtttc gcttccagca
     1381 ggctccaggc ccacggaact cgctggggcg ctataaattc aatatgccga gttcagaggc
     1441 catttatttg catgacacgc cgaaccacaa tctgttcaag cgtgatacac gcgcattgag
     1501 ctcaggctgt gtacgagtga ataaagcttc cgatctggcg aatatgctgt tgcaggatgc
     1561 aggatggaat gacaaacgta tttctgatgc gctgaagcag ggtgatacac gttacgtcaa
     1621 tattcggcag tcgattccgg tgaatctcta ctacctgacg gcctttgttg gtgcagatga
     1681 tcgtacccag tatcgtacag atatttacaa ttatgatctg cctgcgcgat ccagctcgca
     1741 aatcgtatcg aaagcggaac aattaatcag gtaaatgaag tagttcgggg aaatcaattg
     1801 tcgtaataat tagcatgatt gggggcgatt ctctgcagcc cccgtcactg ctggggttga
     1861 gtcatcttga cgtctgcttt acgggcggtt aaggtgcctc ttgtgcgcca gaagtgcata
     1921 taaacgataa cattgagctg tagacttgat tatcatggac aaattcgacg ctaatcgccg
     1981 caaattgctg gcgcttggtg gcgttgcact cggtgccgcc atcctgccga cccctgcgtt
     2041 tgcaacactc tctaccccac gcccgcgcat tttgacactc aataatcttc ataccggaga
     2101 gtcaatcaaa gcggagtttt tcgatggcag aggctatatt caggaagaat tggcaaaact
     2161 taaccatttt ttccgcgatt accgcgcgaa caaaataaag tccatcgacc caggattatt
     2221 cgaccagttg tatcgcctgc aagggttgtt aggcacgcgc aaaccggtgc aactcatttc
     2281 cggttatcgt tccattgata ccaacaatga actacgcgcc cgcagccgtg gagtagcgaa
     2341 gaaaagctat cacactaaag gccaggcgat ggatttccat attgaaggta tcgcgttaag
     2401 caatattcgc aaagccgcgt tatctatgcg cgcaggtggt gtaggatatt acccacgtag
     2461 taactttgtg catattgata ccgggccagc acggcactgg tagtaatcgc ttaacgaaac
     2521 aggggcagta tgaactatcg tattattccg gtcaccgcat tctcccagaa ctgttcattg
     2581 atctggtgtg aacaaacccg cctggccgca ctggtcgatc ctggcggcga tgcggaaaaa
     2641 atcaaacagg aagttgatga cagcggcctg acactgatgc agatcctgct gacgcatggt
     2701 catctggacc acgttggcgc agcggcggaa ctggcgcaac attacggcgt gccggttttt
     2761 ggcccggaaa aagaagatga gttctggctg caaggcttgc ctgcgcaaag tcgtatgttt
     2821 ggtctggaag agtgccagcc gctgacgcca gatcgttggc tgaacgaagg cgataccatc
     2881 agcataggga atgtgacttt acaggtgtta cattgccctg ggcatacgcc gggtcatgtc
     2941 gtgttttttg atgatcgggc aaagctgctg atttctggcg atgttatttt caaaggcgga
     3001 gtagggcgca gtgacttccc gcgtggcgat cataatcaac tgatttcttc aatcaaagat
     3061 aaattgctgc cactggggga tgacgtgaca tttattccgg gtcacggacc attatctaca
     3121 cttggttatg agcgcctgca caatcccttc ctgcaagacg aaatgcccgt ctggtaaagg
     3181 cgcataaaaa agtccacttt taatgcgggc ctggatttct ggcaaagtgc gctttgttta
     3241 tgccggatgc ggcacgagcg ccttatccgg cctacaaaat cgtgcaaatt caaaatattg
     3301 caggggacgc gtaggcctga taagcgtagc gcatcaggca atgttgcgtt tgtcatcagt
     3361 ctcagcccgc ttttcagcgg gcttcattgt ttttaacgct tacagcactg ccacaatcga
     3421 ttcgcacagc ggagccatgt tatctggtgt catcccggcc acatttacgc gaccagaagc
     3481 aacagcatat acgccaaact cttcgcgcag acgcagcact tgttcttttg tcagaccact
     3541 gaaggagaac atgccgttct gtttgatgat aaagctgaag tcgcggtttg cacctttttc
     3601 ctgcagcgta ttgacgaaca actgacgcat acgctgaata cgctggcgca tatcagtcag
     3661 ctcttgttcc caaatcgcac gtaatgcatc gctgctcagg atggtggcaa caacagaagc
     3721 gccgtgtgct ggagggttag agtagttagc gcgaatcgcc gctttcattt ggctgaatgc
     3781 gcgatcaacg atttcactgt cggcagcaac cagagtacaa gcgccaacac gctcgttgta
     3841 caggccaaag tttttagagt aggaactggc aacaatcagc tctttatgca tagccgcgaa
     3901 agcgcgcagt ccttcagcat cttcttccag accacgggca aaaccctggt aagcgaagtc
     3961 aaacagcggt aaccagcctt tctcaacgga gagttgtgcc agtgtctgcc attgttccag
     4021 cgtagggtcg ataccggttg ggttatggca gcagccatgg aacagcacta cgtcgccagc
     4081 ctgagcttcg ttcagactgt taatcagtgc atcgaagtca agggtgtgat tttccgcatc
     4141 ataataagcg tattcacgaa cttccagacc tgcagagtta aagacgctct tatggttcgg
     4201 ccagcttggg ttgctcaccc acacacgctt aacgctggta ttttttgcca ggaagtcggc
     4261 agccacgcgt agtgcgccag tgccacccgg agtctgtgcc gtgcgagcac gtttgtcatt
     4321 gatcagggcg ctacctttac caaacagcag ttcctgagtg cagcgaccaa attcaaggat
     4381 gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg agcagatact gttcagcctt
     4441 tttcacgctg gtcagtaccg gggttttgcc cgtctcatct ttatagacac caatcccgag
     4501 gttaattttg ccgggacgtt catcggcacg aaacagatcg gccaggccca gaatcgggtc
     4561 ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc cattatggtt acagaaggga
     4621 agtccgctat cagggtaacg ggagatttac aaaattccaa ctattactga tgaaaacgca
     4681 ggctgttttt gcaagacgtg agattgctct ggaaggcata aaaaaaacag gaccaacgtc
     4741 ctgttttttc ggcatttaac aaagaggtgt gctattagaa ctggtaaacg atacccacag
     4801 caacggtgtc gtctgaacct acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt
     4861 agtcaacata ggtggacatg tttttgttga agtagtaggt tgcgcccact tcaaagtagt
     4921 tcaccagatc aacatcaccg ataccttcta cgtctttcgc tttagatttg gtgtaagcga
     4981 tggacggacg cagaccgaaa tcgaactggt attgcgcaac taacagaacg tcttgcgttt
     5041 tgttggcgaa gccgctggtg tttgtaaatt tattagtgat cggcgtagcg ttacgggttt
     5101 caccgtagtt cgctgccagg tagatgttgt tcgcgtcgta cttcagacca gtagcccact
     5161 gttcagcttt tttaccgttg ccaagaggtt gagcttcttg caggttggta cggtcagctg
     5221 caccataagc accaacgata ccaaagcctt cgtattcgta gctgatagaa ccgccaacac
     5281 cgtcgccgtt agaacggcgt gcagtgtcac gctcgttttt acccaggtac tgaacagcga
     5341 agttcaggcc atcaaccaga ccaaagaagt tggagttacg ataggtagca acgccgccaa
     5401 cacgaccaac gaagaagtca tcgctgtatg cagtatcacc accaaattct ggcagcatat
     5461 cggtgtaacc cagtgcatca taaaccacac cgtagttacg gccgtaatcg aaagaaccaa
     5521 cgtcagcgta tttaagaccc gcgaatgcca gacgcgtttt gttaccagtt tgagcgtcag
     5581 cgccttcaga gttgttaccc tggaagttat attcccactg accataaccg gtcagatcgg
     5641 aattgatttg agtttcccct ttaaaaccaa gacgggcata ggtcatgtcg ccattgccac
     5701 cgtaactgtt ttcaccgtta cccttggaaa aataatgcag accaacagtt ttaccgtaca
     5761 gatctacttt gttgccatct ttgttataga tttctgcagc gtttgcagta cctgctacta
     5821 acagagcagg gacgatcact gccagaatat tgcgcttcat cattatttat taccctcatg
     5881 gtttttttat gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac
     5941 tattgatgag agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt
     6001 ttcgctaccg tgaaagtgct acaaagataa agatttagtt tcaaaaagaa aaaatatgta
     6061 accaaaagta aaaattaagg agctttgtga acaccgtcat atttccatag gaacgtgatg
     6121 atatttacag caatttttat ctatttatat gatttactta tatttaaatt aactaaacgg
     6181 aaattttgtt tctgatggaa actttatcga cctggcacaa aatcctcttt cagacatcca
     6241 gaatgccaaa aaatagtatg aaaattgtgc tatcagctaa gaaaaaacta atccgcaata
     6301 agagtacggg ttattttttg tgcgtaaatg ttgcaatgta atattccgta acaggatgat
     6361 cgttatggcg ggggtgcaaa tagattgctt gacttaacaa acagataact tgacagaaag
     6421 gataaaaaag agccagcagg cgctggctct ttgaggaaga aaatcagaag ctggcgttgc
     6481 gcggagtacg tgggaacgga atcacatcac gcacgttttg cacgccagtt acgtaagcaa
     6541 tcaggcgttc aaaaccaaga ccgaaacctg aatgcggaac agtaccgtag cgacgcagat
     6601 cgcgatacca ccagtaatct tctttattca ggcccatttc cagcatacgc tcgtccagaa
     6661 cgtccagacg ttcttcacgc tgggagccac caatgatctc accgatgccc ggagccagaa
     6721 cgtccatagc cgcaacggtt ttaccgtctt cgttgaggcg catatagaac gctttaatat
     6781 ctttcggata gtttttaacc actaccggtg ctttaaagtg ttcttccgcc agataacgct
     6841 catgctcaga agagagatcg acgccccagt aaaccgggtt ttcaaacttc ctgccgcagt
     6901 tttcgagaat ggtcactgcg tcggtgtaat ccacctgcgc gaaatcggct tcaatgaagc
     6961 gttccagacg tgaaacggca tctttatcta cgcgttcagc gaagaatttc atgtcgtctg
     7021 cgcgttcttc gagaaccgct ttgaagacat atttcagcat ggcttcagcc agacccgcga
     7081 tatcgttcag gttagcaaac gccacttccg gctccagcat ccagaattcc gccaggtgac
     7141 ggctggtgtt ggagttttca gcacggaaag tcgggccgaa ggtataaatt ttggataatg
     7201 cgcaagcgta ggtttcgccg ttcaactggc cagatacggt caggaaagac tctttaccaa
     7261 agaagtcttt gtcgaaatcc actttgccct gatcgttacg cggcaggttt tccagatcca
     7321 gcgtagaaac gcggaacatt tcgcctgcac cttcggtatc agatgcggta atcagtggcg
     7381 ttgaaaccca gaagaatccc tgctcgttaa agaagcgatg cagcgcctgc gccagcgtat
     7441 ggcgaacgcg cgcgacggca ccaatcaggt ttgtgcgcgg acgcaggtga gcgacttcac
     7501 gcagatactc aatgctgtgg cgttttgccg ccatcgggta agtgtctgga tcttcaaccc
     7561 aaccagcaac ttcaaccttg ctggcctgaa tttcaaattg ttgcccctgg cccggcgatg
     7621 ccacgacttt acccgtcaca atgaccgagc agccggtggt cagacgcagg acgtcttcat
     7681 tgtaattggg cagagaatta ttgatgacag cctgtacagg atcaaagcag gaaccgtcat
     7741 aaacggcgag gaaggagatg ccagcttttg aatctcggcg ggtacgtacc catccgcgta
     7801 cggtgacttc gctgtcaacg gctacgcggc cctggagtac gtcggctaca ggcacaacgc
     7861 tcataatatt ctctctgtta atagtcggaa aaaataaaca cttgtccacc cgtaaatggg
     7921 ggggatacct atg
//
LOCUS       DAJTYL010000151         7908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYL010000151 DAJTYL010000000
VERSION     DAJTYL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.151"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(453..1649)
                     /gene="hemY"
                     /locus_tag="OO572_003538"
     CDS             complement(453..1649)
                     /gene="hemY"
                     /locus_tag="OO572_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6662407.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1652..2851)
                     /gene="hemX"
                     /locus_tag="OO572_003539"
     CDS             complement(1652..2851)
                     /gene="hemX"
                     /locus_tag="OO572_003539"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6662408.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2873..3613)
                     /gene="hemD"
                     /locus_tag="OO572_003540"
     CDS             complement(2873..3613)
                     /gene="hemD"
                     /locus_tag="OO572_003540"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6662409.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3610..4551)
                     /gene="hemC"
                     /locus_tag="OO572_003541"
     CDS             complement(3610..4551)
                     /gene="hemC"
                     /locus_tag="OO572_003541"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6662410.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4938..7484
                     /gene="cyaA"
                     /locus_tag="OO572_003542"
     CDS             4938..7484
                     /gene="cyaA"
                     /locus_tag="OO572_003542"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6662411.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7524..7844)
                     /gene="cyaY"
                     /locus_tag="OO572_003543"
     CDS             complement(7524..7844)
                     /gene="cyaY"
                     /locus_tag="OO572_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6662412.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT         1765 a         2088 c         2051 g         2004 t
ORIGIN      
        1 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
       61 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      121 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      181 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      241 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      301 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      361 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      421 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      481 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      541 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      601 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
      661 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
      721 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
      781 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
      841 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
      901 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
      961 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1021 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1081 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1141 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1201 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1261 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1321 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1381 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1441 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1501 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1561 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1621 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     1681 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc
     1741 ccgctgccgg ttgtgccagc aggttacgca cgcgagtctg catcagtttt tccagcatcg
     1801 cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt aactggtcca
     1861 cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagca cgtacccagg
     1921 tggagacgtt ctccagcgcc tggcgataag tctcttcctg atgacgcggt acagcttgtg
     1981 ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta
     2041 acagcggtac ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaagttct
     2101 gccagctttt ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt
     2161 ctgaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta tctacctgat
     2221 ttgaaagctg attaagctta aggatgatgc cgtcataatc cacctgcgat actgcagaaa
     2281 ggctggcgat atcatcggta attgcccgac gaacggtaat cagactcggg tcattcatat
     2341 ccgccaggct ggcgtctgca cttttcagca acgctgcaga ggtcgtgacg tcctgatcgc
     2401 tccacagctt ccgtccggcg agtttcacca gaaaatcggc ctgagccagc agccaggttt
     2461 tagcatcgct gccggaaatg gtggcgatct tttgttggac ttcatccaac tgttttgcca
     2521 gcgtttcttg ctgacgattc gcctgcttaa gctgcgcagc ttgttgctta ataatgcctt
     2581 ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg ttagccaggg
     2641 catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac aaaccgacac
     2701 ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcagta ttgttcttac
     2761 tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa
     2821 ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta
     2881 atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct tgccagccca
     2941 gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag tgtagtaacc
     3001 agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg
     3061 taacaacgac catcgtcacc tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt
     3121 aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcaccgtca
     3181 gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg
     3241 caatattttg taattcaggt aattgtagca agacttcgct gatttcccga tcctgtgggt
     3301 agagaatctt ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt ccaatggcga
     3361 aataagtagg tagttggggc catttacgat cttgctgatg cagctgtgat tgggcaaaag
     3421 caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct gccagttgat
     3481 cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa tgccaggcca
     3541 cctgccccag tgtgcgcaga cggctcacta actcttctcc agcgggagac gggcgggtga
     3601 ccaggatact catgccgggg cctctccgtt atagacttca gcgaggatct cgcgcgcgcc
     3661 gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatctt gcggcgcacc
     3721 gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg cacatagcca
     3781 gatttcgcca tcaataagct cggcgtagct accaattggc acctgacatc cgccttcgag
     3841 acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc
     3901 ggcaagcagc tcgcgagtgc gtgtatcatc aaggcggcat tcaataccga ctgcaccttg
     3961 tcctaccgcc ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg actccagacc
     4021 taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt tatctagttt
     4081 gctcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg ggcggcgttc
     4141 agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg ctggtaacgc
     4201 atccagactg tcatagttat tggacacaaa ggcatcgcga ggatcttcac gctcgcaaat
     4261 agtgaccagc cccagacctt gcgggaattc aaccggcaca tctttcattg aatgtacggc
     4321 gatatcagcg cgattctcca ggagcgcgac ttccagctct tttacaaata agccttttcc
     4381 gcctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag
     4441 ttcaacgacc aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca
     4501 gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac
     4561 cgtcattatc atccgtggtc catcctaaca tccttgccag agtgatgtca gtgttgtggt
     4621 gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgt
     4681 ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg
     4741 catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt
     4801 cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt gacatacgaa
     4861 atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc
     4921 aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca
     4981 taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct
     5041 acagtctact gccgacattg ttgcactatc accatccgcg aatgccgggt taccttgatg
     5101 gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga
     5161 acgagcttga actgtatcgt ggaatgtcag tacaggatcc accgaaaggt gagcttccaa
     5221 ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc
     5281 tggatatctg ggtctgtcat caatcctggc tcgatagcga agagcgccag ttactacaac
     5341 gtaaatgcag cctgctggaa agctgggccg cctcgctggg tgtggaagtc agcttcttcc
     5401 tgattgatga aaaccgcttc cgtcataatg aaagcggcag cctggggggc gaagattgtg
     5461 gctccaccca gcatatactg ctgcttgacg aattttatcg tactgccgtg cgtctcgccg
     5521 gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agagcattac gacgactatg
     5581 tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat ctcggtggct
     5641 taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta
     5701 tcgattcccc atacaaagcg gtgctgaaaa cactgctgct ggaagcctat tcctgggaat
     5761 acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgagattg
     5821 tatcgtttgg cctcgatcca tactgcatga tgctggagcg tgttactgaa tacctgacgg
     5881 cgattgaaga tttcacccgt ctggatttag tacgtcgctg cttctattta aaagtgtgcg
     5941 aaaaactcag ccgtgaacgc gcctgtgtag gctggcgtcg cgcagtgttg agccagttag
     6001 tgagcgagtg gggttgggac gaagctcgtc tggcaatgct cgataaccgt gctaactgga
     6061 agattgatca ggtgcgtgag gcgcacaacg agctgctcga cgcgatgatg cagagctacc
     6121 gtaatctgat ccgctttgcg cgtcgcaata accttagcgt ctccgccagt ccgcaggata
     6181 tcggcgtact gacgcgtaaa ctgtatgccg cgtttgaagc attgccaggt aaagtgacgc
     6241 tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc
     6301 cgccgggccg ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt
     6361 cgatcatcag ccatcagccg ctggaatata accgttacct gaataaactg gtggcgtggg
     6421 catggtttaa cggcctgctg acctcgcgca cccgtttgta tattaaaggt aacggcattg
     6481 tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct
     6541 tacctgcacc gacaccgaag gcgctctaca gcccgtgtga gattcgccat ctggcgatta
     6601 tcgttaacct ggaatatgac ccgacagctg cgttccgcaa tcaggtggtg cattttgatt
     6661 tccgtaagct ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg
     6721 acctgctgta ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac ggcgagcaat
     6781 cgatgatcga agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag
     6841 atagcgtgga agtcttctgt tatagccagc atctgcgcgg cttaatccgt actcgcgtgc
     6901 agcaactggt ttctgagtgt attgaactgc gcctttccag cacccgccag gaaaccgggc
     6961 gtttcaaggc gctgcgcgtt tctggtcaaa cctggggctt gttcttcgaa cgcctgaatg
     7021 tatcggtaca gaagctggag aacgccatcg agttttatgg cgctatttcg cataacaaac
     7081 tgcacggctt gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg
     7141 gctttgccag cgaagggatc atccagttct ttttcgaaga aacgcaagac gagaatggct
     7201 ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca
     7261 gcaaagagga gctggtacgt gacgtcagtc gcttctactc gtcatcgcac gaccgcttca
     7321 cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg
     7381 atggtcgcga acaggtgatt ccgttccgaa caaaatcgat tggcaacctg ccgcccgcca
     7441 atcaggatca cgatacgccg ctattacagc agtatttttc gtgatgagcg tgccggaaag
     7501 cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc ctgttgcgtc
     7561 gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat ccattcatcg
     7621 cctttcaggt caaaatggta gccgccctgt ttggttgcca gccacacctg gtgcagcggc
     7681 tcctggcggt taataatgat tttgctgccg ttttcaaagg taatggtcag tacgccgccg
     7741 ttgatttcgc agtcgatatc gctgtcgcca tccctgtcgt ccagacgctc ttcaatggtc
     7801 agccacagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt
     7861 taagtgatgg cggcagtata gcggcatggg gtcagggctt caaagttt
//
LOCUS       DAJTYL010000152         7792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYL010000152 DAJTYL010000000
VERSION     DAJTYL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.152"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(66..686)
                     /gene="entD"
                     /locus_tag="OO572_003544"
     CDS             complement(66..686)
                     /gene="entD"
                     /locus_tag="OO572_003544"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6662413.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(861..3101)
                     /gene="fepA"
                     /locus_tag="OO572_003545"
     CDS             complement(861..3101)
                     /gene="fepA"
                     /locus_tag="OO572_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6662414.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3344..4546
                     /gene="fes"
                     /locus_tag="OO572_003546"
     CDS             3344..4546
                     /gene="fes"
                     /locus_tag="OO572_003546"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6662415.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4549..4767
                     /gene="ybdZ"
                     /locus_tag="OO572_003547"
     CDS             4549..4767
                     /gene="ybdZ"
                     /locus_tag="OO572_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6662416.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4764..>7792
                     /locus_tag="OO572_003548"
     CDS             4764..>7792
                     /locus_tag="OO572_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HCR6662417.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHS"
BASE COUNT         1676 a         2100 c         2129 g         1887 t
ORIGIN      
        1 acctgcttta cactttcgcc cgcagtcagt gatggctgcg ggcgaatcgt accagatgtt
       61 gtcaattaat cgtgttggca cagagttatg actatttttt cttttatctg ccagtgcaca
      121 gcaaacatct cattctcacg atgaatgatg acctgctgtt tattccagct aattatctga
      181 tagtccagaa aacctgcatc agtttggatc tcacttgcct taaatgcgct ctctttggcg
      241 gaaaatgcaa gtgtcagcgc cagagaaaag gctaaaccgc agtctgcgag tcgctcgtgt
      301 tctgctggtg taataatgtt gtctgtcaat tcggtcgctg tttgcgcaga aaaaatttct
      361 tcgatatcaa cgccaatcgg ttgacgggaa accacggcca atgccgtagc cccgcagtgg
      421 ctaatactgc cgtatacctc cgcaggccac accggttggc gtagctcgcc gattgcgggc
      481 acacatttat agccatattc ccgcaaggca taaacagcag cgatccgtcc ggctaaatgc
      541 tctgttttac gtttacgtcc agcgtgttgc agttgtgcgt agtgcggcag ccagagtaaa
      601 tcctgctcac aaaaattcgc cggatcgaac tcaacaaaat gcagcgtatg tccggcaaag
      661 gggagggagg tatgcgtagt tttcatatcg accatattcg agactgatga caaacgcaaa
      721 actgcctgat gcgctacgct tatcaggcct acgtgatcct gcaatatatt gaattggcaa
      781 gatctttgta ggccggataa ggcgttcacg ccgcatccgg cgtgaacgac gcgcactttg
      841 tcaacaatct gacgttagca tcagaagtga gtattaatgc tcatatacca cgtacgtccc
      901 ggctcgttat aggtatacgc gccggcaccg gcgatatagt tggctcctgc cagatcgccc
      961 gtggtctggg cattacctgc acgccacaaa cgtttgtcga acaggttgtc cacgccgccg
     1021 gtcagactga cattcttcgt cacatcccag gtcgcgctca gacccacaat gctgtaagga
     1081 ctgatttctt tggtttccgg gccaaccgct ggctgacctt tatagttgta cttcttcggc
     1141 tgctgcttgc cgtaccaggt gaaggtcgtt tgcatcgaca aatcttcccg tgcctgccag
     1201 ctcagcgttg agttcaacgt gtactccggg atgatcgaca aacggtcgcc cgtggttttg
     1261 ttttcactct tcagcatata agtgatgtta ttggtccaca tcaccgtttc gctaactggt
     1321 acgtttaacg atccttccag accttcaacc actgctttcg gcacgttatc ccactgatag
     1381 agatcagtgc cgactgcgtt ttgccctaca gccacatagc ccgcttcaat cttattgcga
     1441 taatcgttac ggaaccaggt gacgcccgcc agccagccgt cgcgtttgaa ctccagacca
     1501 atctctttgt tgatgctggt ttctactttc aggtcatcgt taccttgcag atagcagccg
     1561 cccgcgctgg catagcagcc ctgaccttta ctgtagagaa tgtagttcgg gttagtctgg
     1621 tataggctcg gcgctttata ggcgcgggca atgcctattt tcagcgtgaa gtcatcgcct
     1681 aaaccttgcg atatgttcag cgccgggctc cagttattgc cgacaatact gtgatgatcg
     1741 aaacgcagcc ccggcgttac gatggtgctg tcagtcagct ccatgttgtt ttcggcaaac
     1801 agcgagaaaa tttctgcttt tgaatacggg ctacggtcgg tggcactcac gccatcaata
     1861 gcgccaccgg tattcgttcc ggtcagtgct tgggtgttgg aacttaagtc cttcatccgt
     1921 tgctgattcc actccgtacc cagcgtcagc gtctggttaa cgaggaaatc aatcggcagg
     1981 ttaacttcgc tgtgcagcat cacgtcatcg agatcgatat cgacgaaatc ctgtgtcgct
     2041 ttttcgttaa atttcccttc ggtaccgccc gccagacctt ccggaatacg cgagttacgg
     2101 gtgtgttcat actgcaccca gttgctggtg gtcacgccgt tatcccagcc accgttccag
     2161 gtcagcgcgt agttctggcg atacaggcgg ttggtttcat cgccatattt cgagcgagtg
     2221 taagcgtcag agttggtgtt ctgggtatcg cccgcataca ggttaccctg gcggctgtaa
     2281 cccgcttcca gttccagcga ttgcaatggt gcgaagtccc agcgcaccac gccgttaata
     2341 tctttattga tcaccccttc gcgcccggct ggtaacgtcg tggcatacgt tccggcacgc
     2401 gcggactgat ggccctggtt gatatcccac gcgtcagcct gagttttgtc gaggttgcca
     2461 tacaagcgga agctgaattc gtcgcccagc ggaccggtca ggctaaagtt agtgcgtttg
     2521 gtggcacctt cctctttatg ttctggcgca ttgaaatatg cgtcccagga gccgtgccac
     2581 tcgccgctgc cttttttggt aatgatgtta accacgccgc ccgccgcgcc gttgccataa
     2641 cgcgcagctg ccggaccacg cagaacttca atacgttcaa tcatttcagg tggcacccag
     2701 gaggtatcac cacgggtatc gcgctcgcca cgccagccct gacgcaccga gttacggctg
     2761 cttaccggct tgccgtcaat caaaatcagc gtgttttccg gacccatacc gcgaatatca
     2821 atctgtcggt tattcccacg ctgaccactg gtggagttac cggtcaggtt aacgcctggc
     2881 atggtacgga tgatctccga cacatcgcgg gcaaccgggt ttttgcggat ttcatctgcg
     2941 gtgatggtcg aaacgccagg agcctgcaag ttctgctcgg cggcggtaac gacaatggta
     3001 tcgtcatgtg aaacaggtgt atcggtcggc tcttgtgcct gcgctacccc ataaatcccc
     3061 agattgacca acaaggccag ggaatgaatc ttcttgttca ttgttttatt cctgcatttt
     3121 tgccacgaat tgcaactgtc gggcatggtc gtcatcaaca cgacgcatcc cgttaccgcg
     3181 aaaacctttg atcctgaaag gcacgcagtg cagttggtta attaatgtcc gcgcttccca
     3241 cggcgcgcca ttacgctatt gcaaatgcaa atagttatta ataacattat caatatattt
     3301 ctgcaatcaa tgaaaaattg cacagtaaac atggggttat ggtgtgacgg cgttaaaagt
     3361 aggaagtgag agctggtggc agtcgaaaca tggcccggaa tggcagcgtc tgaatgacga
     3421 aatgtttgag gtcactttct ggtggcgtga tccccaaggt tctgaagaat actcgacgat
     3481 aaagcgcgta tgggtctaca tcactggtgt gaccgatcat catcagaaca gccagcccca
     3541 gtcgatgcag cgaattgcag gtactgatgt ctggcagtgg acgacacaac tcaatgccaa
     3601 ctggcgcggc agctactgct ttattcccac cgaacgcgat gacatttttt ctgcaccatc
     3661 ccccgatcgc ctcgaattac gtgaaggctg gcgaaaacta ttaccccagg caatagccga
     3721 tccgctgaat ccacaaagct ggaaaggcgg gctagggcac gctgtttctg cactcgaaat
     3781 gccgcaagcg cctctgcaac cgggatggga ttgtccgcaa gcgccagaaa taccagccaa
     3841 agaaattatc tggaaaagtg aacggttgaa aaattcacgt cgtgtatgga tttttaccac
     3901 cggcgatgta acagcagaag aacgcccgct ggcagttttg ctcgatggcg aattttgggc
     3961 gcaaagtatg cccgtctggc ctgtgctgac ttcgctgacc catcgtcagc aacttcctcc
     4021 cgccgtgtat gtgttgatcg acgctatcga caccacgcac cgcgcccacg aactgccgtg
     4081 taatgcggat ttctggctgg cagtacagca agagttatta cccctggtga aagttattgc
     4141 cccttttagc gatcgcgccg atcgcaccgt ggtcgccggg cagagttttg gtgggctgtc
     4201 cgcgctgtat gccggactgc actggcctga acgctttggc tgtgtattaa gccagtcagg
     4261 atcgtactgg tggccgcatc ggggcgggca gcaagagggc gtgttacttg aaaagctaaa
     4321 agctggtgaa gttagcgccg aaggtctgcg cattgtgctg gaagcgggta ttcgcgagcc
     4381 gatgatcatg cgggccaatc aggcgctgta tgcgcaatta caccctataa aagaatccat
     4441 tttctggcgt caggttgacg gcggacatga tgcgctttgt tggcgcggtg gcttgatgca
     4501 ggggttaatc gacctctggc aaccactttt ccatgacagg agttgaatat ggcattcagt
     4561 aatcccttcg atgatccaca gggagcgttt tacattttgc gcaatgcgca ggggcaattc
     4621 agtctgtggc cgcaacaatg cgccttaccg gcaggctggg acattgtgtg tcagccgcag
     4681 tcacaagagt cctgccagca gtggcttgaa gcccactggc gtactctgac accggcgaat
     4741 ttcacccagt tgcaggaggc acaatgagcc agcatttacc tttggtcgcc gcacagcccg
     4801 gcatctggat ggcagaaaaa ctgtcggatt taccctccgc ctggagcgtg gcgcattacg
     4861 ttgagttaac cggagaggtt gatgcgccat tactggcccg cgcggtggtt gccggattag
     4921 cgcaagcaga tactttgcgg atgcgtttta cggaagataa cggcgaagtc tggcaatggg
     4981 tcgatgatgc gctgatattc gaactgccag aaattatcga cctgcgaacc aatattgatc
     5041 cgcacggtac tgcgcaggca ttaatgctgg cggatttgca acaagatttg cgcgtcgata
     5101 gcggtaaacc actggtcttt catcaactga tacaggtggc agataaccgc tggtactggt
     5161 atcagcgtta tcaccattta ctggtcgatg gcttcagttt tccggccatt acccgccaga
     5221 tcgccaacat ttactgcgca ttgctgcgtg gcgaaccaac gcctgcttcg ccgtttacgc
     5281 ctttcgctga tgtagtggaa gagtaccagc aataccgcga aagcgaagcc tggcagcgtg
     5341 atgcggcatt ctgggtggaa cagcgtcgcc aactgccacc gcccgcgtca ctttctccgg
     5401 cacctttagc ggggcgcagc gcctcggcag atattttgcg tctgaaaatg gaatttaccg
     5461 acggggaatt ccgccagctg gctacgcaac tttcaggtgt gcagcgtacc gatttagccc
     5521 ttgcgctgac ggctttgtgg ctggggcgat tgtgcaatcg catggactac gccgccggat
     5581 ttatctttat gcgtcgactg ggctcggcgg cgctgacggc taccggaccc gtgctcaacg
     5641 ttttgccgct gggtattcat attgcggcgc aagaaacgct gccggaactg gcaacccgac
     5701 tggcaacccg actggcagca caactgaaaa aaatgcgtcg tcatcaacgt tacgatgccg
     5761 aacaaattgt ccgtgacagc gggcgagcgg caggtgatga accgctgttt ggtccggtac
     5821 tcaatattaa ggtatttgat taccaactgg atattcctgg tgttcaggcg caaacccata
     5881 ccctggcaac cggtccggtt aatgaccttg aactggccct gttcccggat gaacacggtg
     5941 atttgagtat tgagatcctc gccaataaac agcgttacga tgagccaacg ttaatccagc
     6001 atgctgaacg cctgaaaatg ctgattgccc agttcgccgc ggatccggcg ctgttgtgcg
     6061 gcgatgtcga tattatgctg ccaggtgagt atgcgcagct ggcgcagatc aacgccactc
     6121 aggttgagat tccagaaacc acgctgagcg cgctggtggc agaacaagcg gcaaaaacac
     6181 cggatgctcc ggcgctggca gatgcgcgtt acctgttcag ctatcgggaa atgcacgagc
     6241 aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt taaaccaggg gacagcgtgg
     6301 cggtggcact accgcgctcg gtctttttga ccctggcact ccatgcaata gttgaagctg
     6361 gcgcggcctg gctaccgctg gataccggct atccggacga tcgcctgaaa atgatgctgg
     6421 aagatgcgcg tccgtcgctg ttaattacca ccgacgatca actgccgcgc tttagcgatg
     6481 ttcccaattt aaccaacctt tgctataacg ccccgcttac cccccagggc agtgcgccgc
     6541 tgcaactttc acaaccgcac cacacggctt atatcatctt tacctctggc tccaccggca
     6601 ggccgaaagg ggtaatggtc gggcagacgg ctatcgtcaa ccgcctgttg tggattcaaa
     6661 atcattatcc gctcacaggc gaagatgtcg ttgcccaaaa aacgccgtgc agttttgatg
     6721 tctcggtgtg ggagtttttc tggccgttta ttgccggggc taaactggtg atggctgaac
     6781 cggaagcgca ccgcgacccg ctcgctatgc agcaattctt tgccgaatat ggcgtaacga
     6841 ccacgcactt tgtgccgtcg atgctggcag catttgttgc ctcgctgact ccgcaaaccg
     6901 ctcgccagag ttgcgtgacg ttgaaacagg ttttctgtag tggtgaggcc ttaccggctg
     6961 atttatgccg cgaatggcaa cagttaaccg gcgcgccgtt gcataacctg tatggcccga
     7021 cggaagcggc ggtagatgtc agctggtatc cggcttttgg cgaggaactg gcacaggtgc
     7081 gcggcagcag tgtgccgatt ggttatccgg tatggaatac gggtctgcgt attcttgatg
     7141 cgatgatgca tccggtgccg ccgggtgtgg cgggtgatct ctatctcacc ggtattcaac
     7201 tggcgcaggg gtatcttgga cgacccgatc tgaccgccag ccgctttatt gccgatcctt
     7261 ttgctcctgg tgaacggatg tatcgtaccg gagacgttgc ccgctggctg gataacggcg
     7321 cggtggagta cctcgggcgc agtgacgatc agctaaaaat tcgcgggcag cgtatcgaac
     7381 tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt caaacaagcc gttacccacg
     7441 cctgtgtgat taaccaggcg gcagccactg gtggtgatgc gcgtcagttg gtgggctatc
     7501 tggtatcgca atcgggcctg ccgttggata ccagcgcatt gcaggcgcag cttcgcgaaa
     7561 cattgccgcc gcatatggtg ccagtcgttc tgctgcaact gccgcagttg ccacttagcg
     7621 ccaacggcaa gctggatcgc aaagccttac cgttgcctga actgaaggca caagcgccag
     7681 ggcgtgcgcc gaaagcgggc agtgaaacga ttatcgccgc ggcattcgcg tcgttgctgg
     7741 gttgtgacgt gcaggatgcc gatgctgatt tctttgcgct gggcggtcat tc
//
LOCUS       DAJTYL010000153         7772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYL010000153 DAJTYL010000000
VERSION     DAJTYL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.153"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..1322
                     /gene="yjeM"
                     /locus_tag="OO572_003549"
                     /pseudo
     CDS             1..1322
                     /gene="yjeM"
                     /locus_tag="OO572_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OO572_003550"
     CDS             complement(1473..4784)
                     /gene="mscM"
                     /locus_tag="OO572_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6662418.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OO572_003551"
     CDS             complement(4806..5774)
                     /gene="psd"
                     /locus_tag="OO572_003551"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6662419.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OO572_003552"
     CDS             complement(5871..6923)
                     /gene="rsgA"
                     /locus_tag="OO572_003552"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6662420.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7018..7563
                     /gene="orn"
                     /locus_tag="OO572_003553"
     CDS             7018..7563
                     /gene="orn"
                     /locus_tag="OO572_003553"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6662421.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1759 a         2017 c         2102 g         1894 t
ORIGIN      
        1 gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg gatgaataat
       61 agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc ttatatcatc
      121 tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct ctatggtagt
      181 gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt tggtctgctg
      241 gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa taaaattgcc
      301 cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt gctgttagta
      361 agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa tttccttgca
      421 tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt atttgctatt
      481 tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga aaatccagaa
      541 aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg ttattcgctg
      601 gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa tggttccgtt
      661 aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct gggtaacgca
      721 ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga
      781 ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa gccatcatcc
      841 aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc
      901 cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg ctggtttcgt
      961 ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg aacgtgtcta
     1021 tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca cgtcaggatc
     1081 tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg
     1141 tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg gttgaagccg
     1201 gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga
     1261 tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact
     1321 gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc
     1381 gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga
     1441 gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg
     1501 cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg
     1561 aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat cagctggtgg
     1621 atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa
     1681 atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc
     1741 agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg
     1801 gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga ccagttgata
     1861 aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc ccagtcgctg
     1921 atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg gatcgttacc
     1981 gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg
     2041 aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat
     2101 ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag catcagcaga
     2161 tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa
     2221 atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat gataaatacc
     2281 agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc
     2341 gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct
     2401 gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc
     2461 aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc aattgctcct
     2521 tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc taacatctct
     2581 gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag catccagcgg
     2641 cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc
     2701 gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt
     2761 ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc gctgccttct
     2821 ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac cgccagcgca
     2881 ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgagg
     2941 ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta gcgcatccca
     3001 cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca
     3061 aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac accatcgcca
     3121 atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac
     3181 agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag
     3241 aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa
     3301 tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt
     3361 gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc
     3421 gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc
     3481 acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc
     3541 tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc
     3601 tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt
     3661 tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta
     3721 ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc
     3781 tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc ttcgccgagc
     3841 aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc
     3901 tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat ccgctgcgcc
     3961 tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc
     4021 ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg ctccgcctca
     4081 agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg
     4141 ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac
     4201 agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac
     4261 gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg
     4321 atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg
     4381 gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg
     4441 ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg
     4501 cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa
     4561 ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc
     4621 gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt
     4681 gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc
     4741 caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg
     4801 atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg
     4861 atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt ttcggtagat
     4921 actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc
     4981 actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc
     5041 tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg
     5101 atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca
     5161 atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc
     5221 acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg gttaacggaa
     5281 aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg
     5341 cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc
     5401 gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc
     5461 aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc
     5521 agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat
     5581 tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg
     5641 tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg
     5701 ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta
     5761 aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggccaacat
     5821 gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc
     5881 gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag
     5941 ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc
     6001 ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg
     6061 acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca
     6121 ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg
     6181 tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc
     6241 agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa aatgctgatg
     6301 cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct ggaaaccatc
     6361 aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa cgccatgcct
     6421 tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg caaggtttcg
     6481 caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca
     6541 atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa atccggacgc
     6601 gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc
     6661 gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta
     6721 cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca
     6781 aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc
     6841 ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct
     6901 ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat gggcaaaaca
     6961 tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg
     7021 agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag
     7081 cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa tattctggca
     7141 gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat ggatgactgg
     7201 aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc
     7261 gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc tgcgggaaaa
     7321 tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa atacatgccg
     7381 gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg
     7441 cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg
     7501 gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg
     7561 taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc
     7621 taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt
     7681 ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac gacgcagaaa
     7741 tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DAJTYL010000154         7704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYL010000154 DAJTYL010000000
VERSION     DAJTYL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.154"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(123..953)
                     /locus_tag="OO572_003554"
     CDS             complement(123..953)
                     /locus_tag="OO572_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6662422.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO572_003555"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO572_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6662423.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO572_003556"
     CDS             2379..2729
                     /locus_tag="OO572_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6662424.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO572_003557"
     CDS             2855..3415
                     /locus_tag="OO572_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6662425.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO572_003558"
     CDS             3418..6384
                     /locus_tag="OO572_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6662426.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO572_003559"
     CDS             6451..6828
                     /locus_tag="OO572_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6662427.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO572_003560"
     CDS             complement(7029..7688)
                     /locus_tag="OO572_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6662428.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1699 a         2193 c         2089 g         1723 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agct
//
LOCUS       DAJTYL010000155         7699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYL010000155 DAJTYL010000000
VERSION     DAJTYL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.155"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..322)
                     /locus_tag="OO572_003561"
                     /pseudo
     CDS             complement(1..322)
                     /locus_tag="OO572_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(661..1215)
                     /locus_tag="OO572_003562"
                     /pseudo
     CDS             complement(661..1215)
                     /locus_tag="OO572_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1358..1480
                     /locus_tag="OO572_003563"
                     /pseudo
     CDS             1358..1480
                     /locus_tag="OO572_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1742..2308)
                     /gene="ipgB2"
                     /locus_tag="OO572_003564"
     CDS             complement(1742..2308)
                     /gene="ipgB2"
                     /locus_tag="OO572_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6662429.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2590..2904)
                     /locus_tag="OO572_003565"
     CDS             complement(2590..2904)
                     /locus_tag="OO572_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662430.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2982..3092)
                     /locus_tag="OO572_003566"
                     /pseudo
     CDS             complement(2982..3092)
                     /locus_tag="OO572_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3416..4093)
                     /gene="ospD1"
                     /locus_tag="OO572_003567"
     CDS             complement(3416..4093)
                     /gene="ospD1"
                     /locus_tag="OO572_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6662431.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4465..4602)
                     /locus_tag="OO572_003568"
                     /pseudo
     CDS             complement(4465..4602)
                     /locus_tag="OO572_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4684..4982)
                     /locus_tag="OO572_003569"
                     /pseudo
     CDS             complement(4684..4982)
                     /locus_tag="OO572_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5571..5730)
                     /locus_tag="OO572_003570"
                     /pseudo
     CDS             complement(5571..5730)
                     /locus_tag="OO572_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5734..6963)
                     /locus_tag="OO572_003571"
                     /pseudo
     CDS             complement(5734..6963)
                     /locus_tag="OO572_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6983..7330)
                     /gene="tnpB"
                     /locus_tag="OO572_003572"
     CDS             complement(6983..7330)
                     /gene="tnpB"
                     /locus_tag="OO572_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6662432.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7330..7699)
                     /locus_tag="OO572_003573"
                     /pseudo
     CDS             complement(7330..7699)
                     /locus_tag="OO572_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1985 a         1789 c         1676 g         2249 t
ORIGIN      
        1 tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt tatccgccgg
       61 ggcgggagct ttttaatgtc cgtatgacgg tattgatgcc gattttcagc gtacggccag
      121 tatcgcgaac tccggcaccg ttgaaagcca tttcagtgat ttggttttta actcccggtt
      181 tacgggcctc gtaacagtaa gtgagctgaa acacccggtg acagtcacgg caacggaatc
      241 gatcatgtcc ttttgggttc tgcccgtgac ggtaaacctg aacggactgg caacgggggc
      301 aatgaaagtt aactgtgacc atgaaagaac ctaaaagcgg gtattataac ccctaattca
      361 actaattgga ggcattgtca tttttaatct atggatacac ttaaaaatcc tctcacctta
      421 cgttaaaaac cttctcatgt ttagagtttt ttattatgtt tatcataatg atatgacgat
      481 ctgagttttt aaaatcaaga agaaaagata agttttttaa atgcacagtg cctgtggata
      541 actttgcgta aatccggagg acagatcact ctgaagcaag acaacagcat ctgaaaacaa
      601 tatgctcacg tttcaaccac agggcgtaag tgatcctacc cacgtaatat ggacacaggc
      661 ctaagcgagg ttctggtttt caaattgttc cggactgagg ccgccacacc aactgtgccg
      721 tcgccagcga ttgtaatcac attcgatata attaaacact gttgccctca ttatttcccg
      781 gctgataaag tgttctccgt ggagacattc cactttcagc gaatgaaaga agctttccac
      841 gcaggcatta tcgtagcagc agccctttgc gctcatactg ccacgcagat tatgccactt
      901 cagcagcgcc tgataatctg ctgaacagta ctgacttcct cggtccgagt gaacgataac
      961 gttccggggt ctcttacgcc gccacagcgc catctgcagg gcatcgcagg ccagttgcgc
     1021 cgtcaggcgt ggtgacatgg accagccaat aacggcacgt gaccacaggt caatgaccac
     1081 agccagatac agccagcctt catctgtacg taagtacgtg atgtctcccg cccacttctg
     1141 gttcggacca ctggcgtaaa aatcctgctc cagcagattt tctgacacag gcaggccatg
     1201 tgcgcggtaa ctgaccgtat tgctgggtaa gatcaatttg ctcactggta tttactacgt
     1261 gcatcgaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtaagc
     1321 agccggagag tgaaactctc tccggctgac tcgactcgca ggttccgaca gcggtgacga
     1381 acatgcagcg gtgttgttct cgctgatcgg cacatgccgt ctgaacaatg tggagccaga
     1441 aaaatggctg cgttacgtta ttgaacatat ccaggactga ccggcaaacc gggtacgcga
     1501 tctgttgcct tggaaagttg atctgacttc tcagtaaata tcaatatgat tctgacgagc
     1561 cgcttacctt tgagcagaga aaggggagag ggatcccaca tccatgagct aggaaaatac
     1621 aaaaactggc tatacaacaa ttcaatatat taggacttac ttgcgaggag aaatatagca
     1681 ccgctcaggc aattcctggg tatttacttt tcttagtttc tttactcggg atgttctgtc
     1741 atcagaaagg cgattctaaa tttgtaatat agtcactcat attcccacgt aatttcttta
     1801 gatcaatata ttgcacattt ttctcaaaaa actgatcgga aataagattt gcaacattgc
     1861 ttgttgtatc atttttaacg cttctattca tcccttcaca aagttcgtcc attttctttt
     1921 tgaaataact gcttcctgat acaagatgat ttatggaact ttgtgcagca ttgatgtcta
     1981 gagaaatgcg ataatctttc tcaacctgtt taaatatctg tttacggaga ttatcgccga
     2041 tttttcggct gtgttctgca caaaacttat caatacattg gtttaccacc cgggatacgt
     2101 agactttttt ttcatcattt ttccaatctg taatattttc accgatttgt tctgaaacag
     2161 agctcaaaat actagtattt attttggttg atgatatttt tactatcctt ttccccattg
     2221 tacagcggat aatctcatcc acctttcctg aaaaatatct tttgtgagat aaactgattc
     2281 caaaattatt aaaagatgtt ccaagcatta taatcacctt gtcatgtaaa atagtaacat
     2341 tgtcattatt ttagctgatt caaacaattc tgtaaatgct ttgagttctt tactcccttg
     2401 gctcaatgat ggaccgtctg gaatcccaga gactaaactc tctgagaaag agagtatgaa
     2461 aaaacttggg gcagtcccta aatgaaggga ccaacagcgg taaaataacc ggcatcattc
     2521 ataatgatgc cctgttagtt gtagttatga agatttacgt cattaaaatt gctgttcgta
     2581 gtgtcagtcc tatggtctgg catcagctga ggatcgtagc cgatacatca ctggcttcac
     2641 gacattcgtg ttgaggctat tcatgaaaac tccacgctga aaacgccgtt ttgtataaac
     2701 ggtcacggta tgcccggagc cactgttgct gataaattcg ataaaacctt ggcgtttctt
     2761 gaagctatcg ttaatgcgga taatgaaaca acgattggtg agattcgctc ttttgccgac
     2821 gccctggacg ctgtccggtt caatcgcaac aaaatcaacc ggcagctgag caaacccaac
     2881 cttgcctctc tggcgctgga gcatgaagtg atttggctgg gacgtagccg ctgacagctt
     2941 ctataaaagt atgatcctac ccacgtaata tgaacacagg cttaagtgag gttctggttt
     3001 tcaaattgtt caggactgag acctccacac caactgtgcc gtcgtcagcg attgtaatcg
     3061 cacttgatat aattaaacac tgtatatttc atttgtggag tctccagtct gagagggctt
     3121 tatgcattcc gtattgggca ctatgatgcc agaaatctgc aaggctccac accttcctgt
     3181 atttcccttc ctctatcaca gtgcacttta cttaataggg tgggatgtgt tcattacatt
     3241 tattgtgttt tttatgattg tttttgcgtc cagtgtcgtt cgtcatggga aactggttct
     3301 atgggtagta tcgctcagtc tagttaactg tttgtttctt tttagaaatt aagcagatca
     3361 cacaattcgg ggagagatcc ttctaagtgc aattctttgc agctatttag cacacttaaa
     3421 acaactcttg tattttgtca gaaataatat taaacatgtc ttgttcatta ttgttaagtc
     3481 tgtctgctat ttctgtaagt tttatcccac tatgatggca tatatttata tatgccatca
     3541 tagcctcgat attccctttc cctgcggcca gaaataatcc tggtaacccc tcattatctc
     3601 ttacatccaa tagtttaatc gtttcttttg gtgataaatc aaaagtttta ataaaatctc
     3661 cataagcttg aatcgcagcg gaatgacctt cttgaagagc tatgaacaaa gcagattccc
     3721 cagcattatt tcgtgctgca agaacttcta gtttttcctc tttgggtatt tcggctgcca
     3781 acagcgattc accgtattta cgcgctgcat cggcatttcc atgccaaatc aatgaaaaca
     3841 aattatggcg gttatactcc ttattattct ttgtcggaat ggatatgttc tgtattttgg
     3901 ccaattttct gaaagaaaca ccactatatc ccccccctga tttttcttca ttgatggcgt
     3961 gggatatagc ggtatttgtc acgttaatga tattattttt cattccttta ttttcattag
     4021 cagtattgct gcctattatt aagtgcatat ttttgttgtt tgctggatgt aatccatagt
     4081 tatttattga catttttgtt catccttttt gctaaaaaaa aacaataacc tatttttgta
     4141 atgtaataga tattagtcta ttgctaatct aaaaaaacca ttgccacatt gaatggctat
     4201 attcaccaat aaattctttc gatttcgtat attgacatat cgtagatata tcttatccct
     4261 aagattcaat aatcgagtcc gtttaccatt ctgtgcaagc gccttcactc agaagtgacc
     4321 gtcaaggcga tcaccaaact tgataataaa gcagctcatt gccatacgcc agtccttaag
     4381 gggcgttgtc catttctgtg atgctgccta gatttaactg ccagctccgg cttatctaca
     4441 tctaaatatg tgagcctaat accgctacac tttgtccaga ctatgttttt ctccgggaag
     4501 tgggctaacc gtctccataa agtgacgaac ttttttcaac agtggccata ttgaacggca
     4561 ctgatgatta cgggttattg tctcatgaag agccggccac atcactgaca gcagtgactt
     4621 caggccttca gcaccagaca gcctgaacca gttaatccat cgcccgacaa atttagtaat
     4681 ctagggctgg tttttcttca gctgttgcgg ttcgaaagtg ccattacgat cacgcagagt
     4741 acggatttcc agaggttcat ctccagtagt gataatcttc gttaagcaac cattgcggga
     4801 attgctgcca gatctaagct gatttttctc gcacccaaga tgatgagtca tattagcatt
     4861 gagtacggtt tcattttcag catccaatca aactgactga gatcttcagg atttttgaga
     4921 cttttggcta gttcattagc taaagcccgc aacttattag atgtcaactg tttttcattc
     4981 ataaattcac cttcatttga tatcaaatta aatagttcaa catcagagaa ttatacaaac
     5041 ttatgtacag gctcctatga tcttcgaatc ctgaagatta tagtgaaaat aagttttcac
     5101 gttgaaatcc cacatagtta gtatatgata aataatcatg gatgacctga ttaacctctt
     5161 ggggtagtgc ttttatagaa gaataaatat tctactaatt taatacatca cctaaaatag
     5221 tattgttttt tatggttggt tgaaggtcgt ggtgatgatg ttagtccttt tgagaatatt
     5281 ccagtgccag atcttactta taataaaggg gcttggcagt ttggataaac tattgttctt
     5341 attccactaa tacaaacttt ttgtaaagta tattaaatat acttcatttc atactgaaat
     5401 ccccggaagt attcaatcaa acaattacac cataaaagga agagggggcc ctaattaagt
     5461 tgtcaagcat gtttatgata cctgcagggt ataaattttc tggctgcaag cagacacata
     5521 taaaacgtct ttattgatcc tacccacata atatggacac agtcgtaagt gaggctctgg
     5581 ttttcaaatt gttccggact gagaccacca caccaacggt gccgtcgcca gcgattgtaa
     5641 tcgcactcga tataactaaa cactgttgcc cgcactattt cccgatggat aatagtgttc
     5701 tccgtggaga cattccattt tcagcgaatg aattttcatc tgttgctgta tccagtcata
     5761 cagtgactgc atcagtggcg cggctctggc ttttcttgcc gccagacgct gttctgctga
     5821 acatccccgg acttctgcct cgatggcata cagttcaccg atacgctgca gggcttccgt
     5881 ggtgatgtcg gttggcactc ttgcatgcac atcgtggatt tttctccgga catgagccat
     5941 acatgcggct tccgttattc tgccggattc gtacaacgcc cggtaaccac cgtaagcatc
     6001 ggcctgaagc actccgctgt aaccggccag atgattctgt ggatggatac ctttccggtc
     6061 cggtgagtac gcgaaccaga ccgccggagg catttccgaa ccggcgttac ggtcatcacg
     6121 aacgtagacc cacagccggg cagtccttgt tttaccgctg cccggctcct ggaccggaac
     6181 ggggatatca tcggcatgga ctttaccggg catcagcaca tactggcgca ggatgtcata
     6241 cagcggttcc agcagttcag caacagcacc tgtccagcgc cccagtgtgg cacggctcag
     6301 ctccacgccc tgacgacggt atatttctga ctggcggtat aacggcagat agtctgcata
     6361 ttttccagca acaacatggg ccagaagccc cgctccggca taactgcgtg caatgggttt
     6421 tgaaggcacc ggtgcctgca cgatatggtc acaccggcaa caggccagtt tcggacgttg
     6481 tgtttcgata accttaaagg cgctgctgat aagctccagc tgctctgaca catcacatcc
     6541 cagagaactg agttcgccac cacaggccgg gcagcattcc tcttccggac ggatgacccg
     6601 ggtttcacgg ggaagtgagg ctggtaacgg tttacgggct gaagactggc gcaggggaga
     6661 tggcagtacc gggtcatatt gctcacccag cgtttccgcc atttcttcct gaagtgcgct
     6721 gattcgctcc tgtgcttcca gtatctgccg ttcggttttt gcgcgaagtt tttctgagct
     6781 tttaccgaac tgcatgcgct gcagtttcgc aaccagcgcc ttcagccggt tgatttcgga
     6841 agcataagcc gccacccgct gtgagagcag gcggttgtat ttcgccatct ggcggatggt
     6901 gtcctgttgc gtctgcaaca gtgcccgcag gcgggcattc tcatgagcaa gtgaggtgtc
     6961 catatcctca ctttacaacg ggttatatgc ggatgccagc gcgttccgtt cgtttcgggt
     7021 gtttccagtc gatgccctca agaagcatgg ataactgggc cggagtaagg tgcaccttgc
     7081 cgtcacgggt gaccggccag acgaagcggc cccgctccag gcgtctggta aagaggcaca
     7141 gtccgtcact gtcagcccac aacactttta tctggtcacc ccggcgtccg cggaagatga
     7201 acagatgccc ggagaacggg tcatccttca ggacgttctg aacttttgag accaggccgt
     7261 taaagccatt tcgcatatcg gtgatacctg caaccagcca gatacgcgaa cctgcaggga
     7321 aagatatcat cagtggctgc tcccttttat ttcgcggata agtatctgta ataacgccgg
     7381 cgtcagttta cctttaagcc tgagagttcc ggccggcaga accagctcac agcacagact
     7441 gtcggacggt gtatttatct gctctggttc ctgtgccgga gccgggattt tattatccgg
     7501 ctccggcgtt aacgtcacgg gaagcagtgc aggcatattt ttccggaagg cagcaggcca
     7561 cctttccggt attgatggcg ccagttgaag agcaggttat cgttgattcc gttttcacgg
     7621 gcgatctgcg ccacacaggc tccgggctgc agtgactgct ccactaaagc gattttaaat
     7681 tcataaggaa aattaggcc
//
LOCUS       DAJTYL010000156         7635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYL010000156 DAJTYL010000000
VERSION     DAJTYL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.156"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..184
                     /locus_tag="OO572_003574"
                     /pseudo
     CDS             1..184
                     /locus_tag="OO572_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(214..3126)
                     /locus_tag="OO572_003575"
     CDS             complement(214..3126)
                     /locus_tag="OO572_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662433.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3129..3245
                     /locus_tag="OO572_003576"
     CDS             3129..3245
                     /locus_tag="OO572_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6662434.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3609..4451
                     /locus_tag="OO572_003577"
     CDS             3609..4451
                     /locus_tag="OO572_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6662435.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4454..5542
                     /gene="capU"
                     /locus_tag="OO572_003578"
     CDS             4454..5542
                     /gene="capU"
                     /locus_tag="OO572_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6662436.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5547..6497
                     /gene="virK"
                     /locus_tag="OO572_003579"
     CDS             5547..6497
                     /gene="virK"
                     /locus_tag="OO572_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6662437.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6562..7506
                     /gene="lpxM"
                     /locus_tag="OO572_003580"
                     /gene_synonym="msbB"
     CDS             6562..7506
                     /gene="lpxM"
                     /locus_tag="OO572_003580"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6662438.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2062 a         1693 c         1800 g         2080 t
ORIGIN      
        1 aaaaaccgtg gcggcaagcc tgcgccgtca ggggctgagg gcaaaggcct cccggaagtt
       61 cagcccggtc agttaccgcg cacatggcct gcccgtgtca gaaaatctgc tggagcagga
      121 tttttacgcc agtggtccga accagaagtg ggcgggagac atcacgtact tacgtacaga
      181 tgaagttcgg ttacatcctg tcagcacgga acctcacgct ttttgagcgg cacgacggat
      241 cccccccacc cagtatccga cagacttttc actgcgttcg taaaaaccct tcttccctcc
      301 cagcatatcc actaattccg gtgacatatc ctgcagataa gtctgcaaat catgccccag
      361 atcttccggt gactgcaact cataaaccgc gcgccacaca tcacccttcc ttattttaag
      421 ccccgggtta agaatatact gtccgtggtt cagcctcaga aaaagctttt ttccataagg
      481 atttttgctg ttaacttcat gttgagagag ctggctgctg atataactcc ttcgtttgcg
      541 gcgttccggc agcaacgctt caggaagatg aaccacacac tctgccagtc gtgccgcact
      601 gaaggcttcc gaaaaaaaca gagcacttcc cagatgcgtg tggaacagcg cctgcatcag
      661 gatcaccaga aacattgcca tctgccggtt gtccagtttg atgaggcgtc cttccacctg
      721 cagtgacaga ctttcaggtt tcagcagggt atacaactgc gcacttttct gttgcgcata
      781 acggggcgta ctgaccgccg ctgacagaac ttgatgataa gccgccagcc cgtcattacc
      841 ggtcagcaag ggatctgcac cacgctccag cagtaactga accagtgcaa tattacctgc
      901 ccgggctgct gacatcagcg gagtaagatt aaacggcgaa cggtgatcaa caccataacg
      961 ttcaatatcc cgcaacacgg caaccgggtt attcattgag tatggccgga aatacttcag
     1021 ctccagtaac tgcagagtct tcgttctggg ctgccttgtg ggcttaaaat cttcccggta
     1081 cagtgcatta tacagcggga acagggaata aatcagactg tactccagcg cctgcagttg
     1141 tatggtttta tcggctgtac ctgcgaggat atgctgtttc cactgtcgtg catcctctgc
     1201 ggtgataatc tgccatggca tctcagatgt ctgcaggatc cggctacgga tggtgtccgc
     1261 ctgctcctgc ttcccctgtt tttcaagcag atgtgcggtt ttctgccact catcacggct
     1321 ggaaatctct tcctgcagat tcagaggctc ttcctgatgc gtgagcgcaa gcagactcca
     1381 cagaggatgc tccacctgct gttcaacaag atacacattg tgtttagcac gggtgagcgc
     1441 aacatacagc gcgttggtaa aaaatttata gatttccgct gaacgatcct gcctgtcccg
     1501 cggacgggaa taacgggcct cgccctcaag atcagccggt gtaagaccct cgcaaatatc
     1561 atcaaatgcc tgccgggcag cgcttactat attgtaaagg atcaccgttt cgtattccag
     1621 ccccttaacc tcctgaaccg aaaagaccag cggcgtactg aaccagcatc gcgcctgtgc
     1681 tttctgttcc ggatgcatca caatgaccgc tacacgattc gaaagtgagg ttttggcatt
     1741 cagttcctgc tttgtctctt ccctatcgtc cagcaaccgg atggtccctt ctgcctgacc
     1801 gcagcttctg acaaaatggt gactttcccg atcaatagca ctaaagcgga cctgtttgag
     1861 tctcagcaac cggttagcca gtgcagtgac gtggtgcccg ttacggtaat tcgcctgcag
     1921 gatattaacg gttgtgtcgt ttccctgttg ctgccggaaa aagagacttt tgagcgaact
     1981 ccatgagaaa aagctcggat gaactatctg attggcatca ccacagagta aaaactgacc
     2041 gggatgacgt agcgttttca gtaccagttg cagttgaaca ggcgtcatat cctgaacttc
     2101 atcaacaaaa atgacatcat aacggggagt tgccaacgaa agatactgat gactgagcag
     2161 attactgtca tactgatgcg actgtttcag ccatgcgata taacgttcaa acaggacata
     2221 gaccgtcggg cggtcctcca tgccatagag cgactgccgt atccccagtg acagatacgc
     2281 ctcccgggac agcggcccat tcccggaggc aaccgcaccg atcacgcccc tgaactcctc
     2341 atacagggta tgggcagccc ccagtgcggc gatagccctg ttgcggggca gccagtcgct
     2401 gaacgcactg aaagtcacct ccctgccttc cggaatgcgc agagtttcca gaaattccgt
     2461 cagcgaaaga aagtcaatat tctgtacctc actcccttcc cctgaagcgt catagagagt
     2521 acgcgctttt tccaccagaa atgaggaaag gctgagatac agtatatcac cagcagcctg
     2581 cttcattttt tccagtacca gcgccgtctt accacttccc gccgagccaa caatcaccag
     2641 cggtagcggg tgttcgtaca gtgcctgctg atcatcatca aaggacagca ttttatccag
     2701 gcggtggaaa cgcccatgag acggattgat atacgtcagc gcttcagtcg cgatatcaac
     2761 aggatccggc actggttgtt gttgtaagcg cccttcatca atcaccacgt ttctgcgaag
     2821 aaaacgtgaa gtgttatagg catgattgcg aatatattcc agaacaaggc agatagtttc
     2881 accgtggtag cgatacagcg aaaacagtaa tcggtcccgg atactgagcc gggcgcggta
     2941 aagattggtg tcaattttac gaacatcagc ctgaacaaag ttatcggtgg caatagcctg
     3001 tgcaaatttt ttataacccg ggatcgtgtc aggattcaga gtatgataaa gacagatttt
     3061 catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt ttacgaagga atgccttact
     3121 gaacaacctt gcccggaata ccccccgaaa tcagatggaa caatcagaaa aatgctctgc
     3181 agttatggaa taaagaaggt ggggtggtac gccgggaggg cgatccgctg gaaatggcgc
     3241 agtagctgca ggcctgtcat gatgcaggaa tagaagttcg tgtacaggtt aatgaaagcg
     3301 tcaccccgta aataaactct gcgctgaaat tccttttttt cattggagta cctgtgttgt
     3361 tctgaggtga acatatcacc tctgttcagg tagccaaatt cagtgtgcca cctcaaggtt
     3421 cttagtgagt cgtttttaaa agatatgata atatatcatt gtttatgaat tgttgatgtt
     3481 taatttagta aattatatca aatatgccat gggtttgcta tgttattcat ccagtatctg
     3541 gaaataaaat gcaccagctt tattgggttt tccttttgtc tctgcttgct aggtgccaga
     3601 aactttaaat gttaaacgaa gggggcattt tgttcaaagc caatcatgtt ccggtattaa
     3661 tgtatcatca tgtcagtcat tgccccggac tggttacact ttctcccgtg acattccgga
     3721 agcaaataaa atggttggct gaaaacaact ggaaaacact gtcatcagat gaactggagt
     3781 ttttttatcg gggcggaaaa ctcccccgga aaagcgttat gttgacattt gatgatggat
     3841 accttgacaa ctggtttcag gtatatcctt tattaaaaga gtttaatctc aaagcacata
     3901 tttttttaat taccggtttt attgggaatg gtccggttcg tcactctcct ggaaaggaat
     3961 attctcatcg cgattgcgaa catcaaatag cgacagggaa tgctgataat gttatgcttc
     4021 gctggtctga agtaaacgaa atgttgcaat ctggtctggt cgaatttcat gttcatacgc
     4081 atactcatac acgctgggat aaaaagttca gttcacgaga agaacagtgt aaacatcttc
     4141 ggcaggactt gttgagcggg agggaatatt tgaaagaaat gacaggaaaa tgcagtaaac
     4201 atttgtgttg gccggaaggt tattataaca aggattatat tcaggtcgct gaagaacttg
     4261 gattctatta tttatataca acagaaagaa gaatgaatgc tcctgctaaa ggaactacac
     4321 ggataggaag aataagcacc aaagaacggg aaagttgcgc atggctgaaa agacgacttt
     4381 tttattatac aaccccgttt ttttcatcgt tgctggcctt tcataagggg ccaagattac
     4441 cggatgatta ataatgaata tactatttac ggaatcatca ccgaatatag gtggtcagga
     4501 attacaggct gttgctcaaa tgaaggccct gaagaaaatg gggcattcag ttctgcttgt
     4561 ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa ttggggattg atatcacatt
     4621 cgcgttattt cgaaacagtc ttcacatccc tactgcatgg agattactcg gaatagttca
     4681 tggttttcag cccaatgcaa tcgtttgtca cagtgggcat gatagcaata ttgttggttt
     4741 agtacggtta ttcactagga aacatccatt cagaattatc aggcaaaaga catatttgac
     4801 acgaaaaaca aaagtttttt caataaatca tttttgcgat gaggtgattg ttcccggaac
     4861 aagtatgaag acacatctgg agcaggaagg atgtcgaacc cgggttactg ttgtgcctcc
     4921 aggctttgat ttccagaaat tatacgttga ttcccgaaac agtttgcctc caaatgttct
     4981 ttcttggctg gcgtcccgaa ggggatgccc cgttattgct caggtaggaa tgttgcgccc
     5041 ggaaaaaggg cacgaattta tgttgaattt actgttccat ttaaaaatga atggacgaca
     5101 gttctgttgg ttgattgtgg ggtctggttc gcctgaactg cgggagcatt tacagtatca
     5161 gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc
     5221 ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat cgtttggtat
     5281 ggtactggca gaagcatcgg cattttctgt gcctgtactg gccagtcaga ttggtggaat
     5341 ccctgatgtt attcagaata accagaccgg gacattgtta ccagcaggta ataagcacgc
     5401 atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg
     5461 ccaggcaaaa caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact
     5521 cacattagcg aagcacaagt aacaatatgt tttctgtaag taacttatca tttatcggtt
     5581 tccttaaaag gattgttttt tcctcagatt cactcccggg gaagtgggaa cacagaaaat
     5641 tccggttcat gtacattttg cgatgtgcta taaatccggt tgccagtatt cgatattatt
     5701 acgaactgcg ttccttgcag tgcattgagg atattctggc aatacaaccc acgttgccag
     5761 caagaattca tcgaccttat ttgcataaag gggggcgagc ctggtcccgg gggcaatata
     5821 ttcttgagca ttatcgcttt gtgcagaatt tgccagaaaa atattctgaa tttttgtttc
     5881 cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagaggat tttgatatcc
     5941 agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg tttttcaata
     6001 aaatagtgat tgcccggtta acattttcag tcattctgac tcaaaatgga cacaccgctt
     6061 ttattggcgg attacaggga gcccccaaaa ataccggacc tgatattatt cggtgcgcaa
     6121 cccgggcctg ttatgggctt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga
     6181 tgaaagcttg taatgtctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc
     6241 aattgagata ctggtatcag aaacgcaaaa cctttgttgc tgtctacagt gatttctggg
     6301 agtctgtagc agggaaaacc tgtggtgact ggtacaaatt accaacacag gtggttcgaa
     6361 agccgctaag caatatagct agtaaaaaac gctctgagta tcgaaaacga tacgctttgc
     6421 tggattatat ccatgaaact gctatccgct ctttggatgc atatcctgta aactcagaac
     6481 atcaggactt aaattaaggt ttgatgtatt ctctgaataa aatattaatg atgattttgg
     6541 taggggcatt cgcactaaat aatgaaaaaa tacaaatccg agtttattcc tgaatttaag
     6601 aaaaattatc tttcccctgt ttactggttt acatggttcg ttttgggaat gattgcaggt
     6661 atttcaatgt ttcccccttc attcagagat cctgtcttgg ccaaaatagg gcgttgggtg
     6721 ggcagattga gcagaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttcccg
     6781 gaaaagagtg atacagaacg ggaaataatt gtcgacaata tgtttgccac agcattgcaa
     6841 tccatagtga tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg
     6901 ttctggaaag gcctcgaaat tcttgaggaa attcggcaca ataacagaaa tgtgattttt
     6961 ctggttcccc atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggggaa
     7021 aaaatggccg ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattct
     7081 gtacggcgta aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt
     7141 cagtcagtac gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa
     7201 tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt
     7261 ctgatgaaca tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa
     7321 catttcctga ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat
     7381 aaaatgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa
     7441 cagtatatct gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac
     7501 ccttgaaatt ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa
     7561 acacatcccc cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt
     7621 taaatttaat ttatg
//
LOCUS       DAJTYL010000157         7472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYL010000157 DAJTYL010000000
VERSION     DAJTYL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.157"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1090)
                     /locus_tag="OO572_003581"
     CDS             complement(<1..1090)
                     /locus_tag="OO572_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6662439.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQ"
     gene            complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO572_003582"
     CDS             complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO572_003582"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6662440.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO572_003583"
     CDS             complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO572_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6662441.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO572_003584"
     CDS             complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO572_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6662442.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO572_003585"
     CDS             complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO572_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6662443.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO572_003586"
     CDS             complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO572_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6662444.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3394..4371
                     /gene="pspF"
                     /locus_tag="OO572_003587"
     CDS             3394..4371
                     /gene="pspF"
                     /locus_tag="OO572_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6662445.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO572_003588"
     CDS             complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO572_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6662446.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO572_003589"
     CDS             complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO572_003589"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6662447.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7076..>7472)
                     /locus_tag="OO572_003590"
     CDS             complement(7076..>7472)
                     /locus_tag="OO572_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6662448.1"
                     /translation="QLLLDGRNAGLAAAIGPTIFVDVDPNASLSREEIFGPVLVVTRF
                     TSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVFVNNYNDGDMTVPFGGY
                     KQSGNGRDKSLHALEKFTELKTIWISLEA"
BASE COUNT         1741 a         1991 c         1897 g         1843 t
ORIGIN      
        1 cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag ccacgcagtg
       61 ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt ttactggagg
      121 gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt tgcaccgcat
      181 gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca tcaccgttgc
      241 caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc actgtacctg
      301 gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt ttttccagat
      361 ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg cggacatact
      421 cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac acttcagggc
      481 tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg cgcgtggaat
      541 tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg acggcactga
      601 gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc tgttgtaaat
      661 agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca aacattaaat
      721 gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat
      781 gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt aataaatgaa
      841 catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg ggtagtggtt
      901 ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc
      961 agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt
     1021 tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa tccgtaattt
     1081 tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact actccgcaga
     1141 aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg
     1201 gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata aggtgtgaca
     1261 gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt gaccttcggc
     1321 attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg
     1381 agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
     1441 ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc
     1501 ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg
     1561 gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt taaacatgta
     1621 agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta acaatttttg
     1681 cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg acttaacagc
     1741 ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga ttttatcgcc
     1801 cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag cttgcctgct
     1861 aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt cataactttc
     1921 ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca taacgttcca
     1981 tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc agcaattcgc
     2041 tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca agcgcaaatg
     2101 acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc gacagcacca
     2161 ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg gcgcagacgc
     2221 cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta atgcccgcca
     2281 ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg cctgaatacg
     2341 ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct cactttgcga
     2401 caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg gtaaaacaaa
     2461 cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta ctccttagac
     2521 gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc ttgcttcatt
     2581 ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag ttcggcaaac
     2641 tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg
     2701 tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt gccactgtcc
     2761 agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat caatgcctgc
     2821 tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt cttcatgcgt
     2881 gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag atcggtcagt
     2941 ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag cagcgccagt
     3001 tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg gcgagtcagc
     3061 tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag tgtatcttcc
     3121 atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc taacagagcg
     3181 ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat gttgtcctct
     3241 tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt gccaattttt
     3301 tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc aggatgaatt
     3361 agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga taatttactt
     3421 ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc accgctggac
     3481 aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc cagccgcctg
     3541 cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc ggcgttaaat
     3601 gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac cggtgcgcaa
     3661 aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct tgatgaactc
     3721 gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta cggtgaactg
     3781 gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg cgcgacgaat
     3841 gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct cgaccggctg
     3901 gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat tatgctgatg
     3961 gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt
     4021 acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat tcgtgaattg
     4081 aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc gcttgatgac
     4141 atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt ttcagaaaat
     4201 acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca ggaaaaagag
     4261 ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc tgaattactg
     4321 gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta gcgaagttga
     4381 gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa tcaatacgtc
     4441 gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta ttaatcgctc
     4501 agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga
     4561 tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag cccctgcgcc
     4621 agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt ttgcggatcg
     4681 ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc cggaacgctt
     4741 tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc gcgttcgcag
     4801 agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc cagcgggttc
     4861 ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt cgcgttaccg
     4921 accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat taagtccggc
     4981 ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc gctttgcact
     5041 tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc ggcaaccagc
     5101 tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat aatggccgcg
     5161 acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag ggagtcctgt
     5221 gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc agggaacggg
     5281 ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat atatgtacga
     5341 ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc gcgagcaatt
     5401 ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt agcctgcccg
     5461 ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc gtacggcaca
     5521 atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc gaccagatca
     5581 gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat gtattcgttg
     5641 ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt acacatcacg
     5701 ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt gctcatgttt
     5761 cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct
     5821 gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg
     5881 gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca gcagttcagc
     5941 aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac cctgcatgta
     6001 atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca gcaggaagtt
     6061 gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt ttagtagttt
     6121 cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta cacccccgcc
     6181 gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc
     6241 cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc gttcggtggt
     6301 gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta ctttatcgcc
     6361 cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat tggcagtccg
     6421 aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat acacgcgccc
     6481 gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt gaatatgccc
     6541 accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc ggcgaatagc
     6601 gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct ctttctgctc
     6661 ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg ggcggtagtc
     6721 gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct cgaacatcat
     6781 gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt cgatgtcgcg
     6841 gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc cgatgcgtga
     6901 ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg aggagagccc
     6961 ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca gtgtgtcgaa
     7021 tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg
     7081 cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca tgcagggatt
     7141 tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt
     7201 tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg tgcgcgcggg
     7261 agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg gcaagctgca
     7321 gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa atctcttcgc
     7381 ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg gccgctgcca
     7441 gcccggcgtt gcgaccatcc agcagcagtt gc
//
LOCUS       DAJTYL010000158         7280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYL010000158 DAJTYL010000000
VERSION     DAJTYL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.158"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..219
                     /locus_tag="OO572_003591"
     CDS             <1..219
                     /locus_tag="OO572_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HCR6662449.1"
                     /translation="RLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHA
                     LPEQVYFVCYDEENAHLYERLLTQQGDE"
     gene            161..1642
                     /gene="clsC"
                     /locus_tag="OO572_003592"
     CDS             161..1642
                     /gene="clsC"
                     /locus_tag="OO572_003592"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6662450.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1650..2807)
                     /gene="mdoC"
                     /locus_tag="OO572_003593"
     CDS             complement(1650..2807)
                     /gene="mdoC"
                     /locus_tag="OO572_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6662451.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3201..4736
                     /gene="mdoG"
                     /locus_tag="OO572_003594"
     CDS             3201..4736
                     /gene="mdoG"
                     /locus_tag="OO572_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6662452.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4729..7272
                     /gene="mdoH"
                     /locus_tag="OO572_003595"
     CDS             4729..7272
                     /gene="mdoH"
                     /locus_tag="OO572_003595"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6662453.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1799 a         1746 c         1920 g         1815 t
ORIGIN      
        1 cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag tactggggtt
       61 tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc agaatttatt
      121 acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga aaacgcccac
      181 ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc tggcgagcgc
      241 ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc tggagaaaag
      301 tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata cgctggatgt
      361 tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg ccctgttagc
      421 cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata cgcccggact
      481 tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc tttttaatcc
      541 tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc gtctcaatcg
      601 ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg gaggacgaaa
      661 tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt tagatgtcat
      721 ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact ggtattgcaa
      781 atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg cggatcgcat
      841 cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac gcaaaatgga
      901 atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct gggcgaagac
      961 acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc attcactgtt
     1021 accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata ttatctcttc
     1081 ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga gaaaaggggt
     1141 aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg tccatgccgg
     1201 atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg aactcaagcc
     1261 gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt ccggtgccag
     1321 cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct ctttcaattt
     1381 cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga gcgaaacgct
     1441 ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct ggcagctccg
     1501 tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag aggttattct
     1561 caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg cgtcgatatt
     1621 gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc gttctcacgc
     1681 ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa atgaatttca
     1741 tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc aagccagttg
     1801 gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg gtgaaccaga
     1861 tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa gttaagcaaa
     1921 cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat ggtgatcacc
     1981 gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag taaataagcg
     2041 acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa caaggcttta
     2101 agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata aaatagcgtt
     2161 tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac aataaaaatc
     2221 gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga tagttttacc
     2281 atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat gcgcttaaat
     2341 atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag gtgtgatatt
     2401 aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca actttccgct
     2461 ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg gatggctgtt
     2521 aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa gggatagcgt
     2581 aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat gcggaacgag
     2641 tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt cacatgccat
     2701 gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat cagccaggcg
     2761 cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata tggttaacta
     2821 atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt gccatagcga
     2881 caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca tcatcgctac
     2941 cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct ggcaaaccct
     3001 acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg gttgcaatca
     3061 ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt cggaatggct
     3121 ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag tgcttactaa
     3181 ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca gtaatgttaa
     3241 ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag caagctcaat
     3301 ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt ttccgcgaca
     3361 tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac tggaacaatc
     3421 tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat accccggtca
     3481 aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg gattatttca
     3541 ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt gccggtttta
     3601 aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc atgctcgggg
     3661 ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc cgcggcctag
     3721 caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag ttctggatcg
     3781 agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac tcgccgcgtg
     3841 cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg gatgtgcagt
     3901 cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta accagtatgt
     3961 tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag ttgcacgact
     4021 ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg ttgaataacc
     4081 cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt ggtctgttgc
     4141 aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat ctccgtccaa
     4201 gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg gtggaaattc
     4261 caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat cagctgccgg
     4321 agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat gaagacaaac
     4381 tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg gatgtgaagc
     4441 agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat tttaccggcg
     4501 cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc attggtgata
     4561 atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc tggcgtctgg
     4621 tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct gcgctggtga
     4681 atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat gaataagaca
     4741 actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt gccgaagact
     4801 gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc gcgggaggat
     4861 gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga ttcacttgct
     4921 gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat gccagaagca
     4981 aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg tttctgggat
     5041 cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa agaagagcag
     5101 gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct gttgatcctg
     5161 acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc ttatcagggt
     5221 tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc ctttatgcag
     5281 cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact gttctgttgg
     5341 gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat tggtcgcgat
     5401 aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga gcatcgcacg
     5461 gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg cttgcgtgca
     5521 acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta cattctgagt
     5581 gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga gcttatcgct
     5641 gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt gaagcgtaaa
     5701 agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta catggtggtg
     5761 ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg cttgatggaa
     5821 gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat ggatacgctg
     5881 tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac agccggtttg
     5941 cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat ccgcgtgaaa
     6001 ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt tgccggttca
     6061 atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg gggggtctgg
     6121 attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct tgatgagcta
     6181 aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt cctggttaag
     6241 ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta tctctccgct
     6301 ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca tgcgttgacc
     6361 gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca gtggcgtcct
     6421 gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc gaagttattg
     6481 agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg gcgcgttaca
     6541 ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg catgctgttc
     6601 catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa ttcaccgcag
     6661 cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc acagctgctg
     6721 ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct gttctggctg
     6781 gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc cagccgtgcc
     6841 accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga gtattcaccg
     6901 ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg ctcccttgac
     6961 gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac cgcaatggcg
     7021 acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca cgttgaacag
     7081 gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct gctaagcgat
     7141 ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag atattcttca
     7201 tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa accggatgcg
     7261 gcttcgcaat aaaaacgtag
//
LOCUS       DAJTYL010000159         7260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYL010000159 DAJTYL010000000
VERSION     DAJTYL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.159"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..499
                     /locus_tag="OO572_003596"
     CDS             <1..499
                     /locus_tag="OO572_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6662454.1"
                     /translation="IKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQ
                     SINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKS
                     TLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACALANK
                     LARIA"
     gene            complement(838..1608)
                     /locus_tag="OO572_003597"
     CDS             complement(838..1608)
                     /locus_tag="OO572_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662455.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1653..2087)
                     /locus_tag="OO572_003598"
                     /pseudo
     CDS             complement(1653..2087)
                     /locus_tag="OO572_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2099..2320)
                     /locus_tag="OO572_003599"
     CDS             complement(2099..2320)
                     /locus_tag="OO572_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662456.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2321..3004)
                     /locus_tag="OO572_003600"
     CDS             complement(2321..3004)
                     /locus_tag="OO572_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6662457.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3068..3379)
                     /locus_tag="OO572_003601"
     CDS             complement(3068..3379)
                     /locus_tag="OO572_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662458.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3376..4278)
                     /locus_tag="OO572_003602"
     CDS             complement(3376..4278)
                     /locus_tag="OO572_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6662459.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4718..5677
                     /locus_tag="OO572_003603"
     CDS             4718..5677
                     /locus_tag="OO572_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6662460.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5677..6072
                     /locus_tag="OO572_003604"
     CDS             5677..6072
                     /locus_tag="OO572_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6662461.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6255..6635
                     /locus_tag="OO572_003605"
     CDS             6255..6635
                     /locus_tag="OO572_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6662462.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6681..7260
                     /locus_tag="OO572_003606"
                     /pseudo
     CDS             6681..7260
                     /locus_tag="OO572_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1963 a         1881 c         1770 g         1646 t
ORIGIN      
        1 aataaaacgg ttgtctctcg tattggctga acacgaaatc cccgaatatc tgagccgcct
       61 tttggtgagg ttgcataccc actaccttta ccttgttgag caaatagcag agctggaatc
      121 agaattaagt cagtcaatca acgctgatga taccgctcag cgcattatga caattcctgg
      181 tgttgggccg attactgcca gcttactatc gtcgcaactt ggtgacggga aacagttttc
      241 atgcagcaga gattttgcgg cttccacagg gctcgttccc cggcagtata gcactggagg
      301 aaaatcgact ctgttgggta tcagtaagcg aggtgataaa aatttacgcc ggttactggt
      361 tcagtgtgcc cgctcattca tgatgcagct tgaacgccag catggaaaac ttgctgagtg
      421 ggttcgggag caactcaaca agaagcattc gaatgtggtt gcctgcgctc tggcaaataa
      481 actggcccga attgcctagg caatcactac tcagcaaaaa gaatatcagg cctgatatat
      541 agatgctgac aaaactcaat tagcttattg caaacaataa gatacaagtt accgtctggt
      601 tttgcgaaga ttgattattg atgacatgaa cggcaaatcg acctgattaa aaacctaaca
      661 ggggaaaatg gctcactgaa gccgagggga tttttaggat tatcaggtgc ggaactcatc
      721 gaggcgcggg catcccttgc ccacagagac gccggataga tttaagcaag cccaccatta
      781 atcaaaaatc agtgttgcaa aaacgggggt gaccatagat tttccccgcc atcgtcatca
      841 tttccggatt tttgccaggg catccgccat gctgcggatt tcggcccggg cttcccggac
      901 aacttcccgc agaacaccac ggtcaaacca ccggcggggc ggttcatccg gtatacgcag
      961 cacatcgcag gccagcccac tggacaggat atgctcaata caatcgctga ccagatacat
     1021 ttccaccgcc gaaaaacgtg cgccccggtt caggatggca tcgagcacca cgggcaggtt
     1081 ttcatcgtct tcatcgagcc agcaggggtg atcgctttcc ttcacgacca ggcgaaagcg
     1141 gcgcgtctgg cgggtcagca acgtctgacc gcttgtggtc agcgccgccc ggatacgccg
     1201 cgcatcttct gccccccggc aactgacact gcatactgca tcgcgcaccg catcaagaac
     1261 tgccctgatt tcgtcttcgg tcagccattc accggcatac gggaaagaca cctgcgttcc
     1321 gtccgccgga agtaccggca gcgactgcca ctgactgccg gacagaacaa gttccgctcc
     1381 ggttccggtc agtttctgaa tggcggaggc caggtgcggc ggcagcacgc catcgtggcg
     1441 gttaacgagt aacgtcgcca gccggggcgc atccagccgc acgtaaatgc tgatccagcc
     1501 cggatagttc gggttacatt ggtcaacatc cgtctcttcc ggccagccct gcgccagcag
     1561 caggttacgg gcgtgacgac aaatcagagc attcagcgtt tcattcataa ttcatgaact
     1621 ccctgtatcg ccgcccacag gcggcgataa catcagtcgt tttcgtgtgt gttgtttttc
     1681 agcaggctgc gggcctgatc aaccatcgcc tgtcggcggg taacgtattc cgggaggggg
     1741 aaaccgtgat cccgggcagc ccattcacgt ttccacagtg cctgttcagc gatctgagtc
     1801 agggtctcca gcagttcagc agggacggtc acctggcggt tttcgcgcac gttgctgata
     1861 atctccatcg ccgcggcgga agaaataccg gactcaatcc gctagtcttc cgggatgtcc
     1921 tccaggtgcg ccaggacggt cctgatttca tgatcggaca gggaaatatc cattccgtca
     1981 gcgagggcct gaatctcttc cggggaccag ataaccagca tcagcggcgt gtctcccgaa
     2041 tactttgctg ccagcgcccg gcatagcgtt tcgcatgttc cgtacatact ctctcccctc
     2101 aatctttgaa gcaggaatcc agttccagaa tttcggtata tgcctccatc aagaggcggt
     2161 ttgcgcgaaa caacgtctga cgcaccagac gttccggcag ggtgtcagca ttaaagctgt
     2221 accgccgctc catctcattc atggttgtac gcaggtcgca catggcattg gccacctccg
     2281 ccacttcggc gcgaagtttt tcgtgtccgg catagttcat ttacgcagcc tccggcataa
     2341 accagtcatc attcgcggcc ccctgctgca tggcccgctg tacggcagcc agtcgttgct
     2401 gtccggcacg gtgatactgc tcaagcagct cgataccgat ataccggcgt ccggactgga
     2461 gggcggcgac gcaggttgaa ccgctgcctg caaacgggtc cagcactatc gcattcgggt
     2521 gtgtgaagct ctcaatcagc ggttgcaggc tggtaacggg cttttccgtc gggtgatggc
     2581 ggttgcccga atatttccag cccaacacgt ccggcagcgg gttttgtggc agacgtggac
     2641 ggccttttgc caggatgtag gcgcattcgt ggcgatagcc cacatatgcg gccttcgatg
     2701 tgtagttttt ggtgaagacc aggtgaccaa caacgctgaa tcccgcattt ttccaggcag
     2761 acataaagcg atcgacgcgg ttccagccat agaagctcac catcagcgcg tcttttttga
     2821 gtacgcggta catttcatta caggccggtt gcagccattc atcagtttta tcaccagcaa
     2881 tggtacgccc ctgacggtca cgaaaaccga cgagatatgg cggatcggtg aggatgaaat
     2941 caatggcgtt atcaggaata cgggccataa catcgatgca gttaccgagg acaaaacggg
     3001 acataacaga tccttctctt acagagggca cccctgtggt gcccgttccc cgctgcctgt
     3061 gggctgttca tcgcttccgg taacgggtca ggccgaccgt ggaataccgc agccgtcgtc
     3121 tttttgacag caaggttatg ccgcgaaagc ggcttgaagg gcgttcatca gaaaagggcg
     3181 acgggctggc gtacactgtc accgacacca gccgggagcg gtaaaagcgc ccgtcccgtg
     3241 cccgccggac gcgggacact gaccacaggc agcgtttatg ccgcgccgcg tcagccggcg
     3301 cagtaaaaag aggagcacgg cgacatcctt tgtcggaaaa gaaacgggcg gttgcccgcc
     3361 cgtctccgtc atacttcatc cgccataatg cgccacattg tcgactatcc acgcaggtcc
     3421 ggtgacttcc ggctgctcat cgtcatctgt cggggaagac cattcaagtt ccccccacag
     3481 cacattgacg agctctccac gctcatacaa ctgccagcca cagaaatcac agccctgttc
     3541 ggcataccag tgctccagcg tgcagctgaa acggcggctt aattctgcaa taacgtcgct
     3601 ttccggctga caccacggag tgtcgaaatc cacctgaatg aagttttctc cctgactgct
     3661 gatattcagg tcatatccac aaggccactt cacgccatac cgttccgtat accagtgata
     3721 tgcagaagga acaccgttca gaacaccacc attaaagccg ttcacttcaa catccaggcg
     3781 ggtcggcaga accgtcagca ggtcaaacgg catggttttt tccggcacta cgttgtcaca
     3841 cagacggttc caccacaccg atacatcctc gttaccccat atgtcacacc agtcacctct
     3901 ttttgcggtg aaatgcacag tgatggtttc cctggcatca tccggtaatc cttcccaacg
     3961 acgctggcca gtaccgctct gtaaccagag ttcatgcagc atcaggcagt tttgttcatc
     4021 cagcaacaca ccgttctgca ggtgtgtcag ccagcgggtg aacgcgatat tttccgtcga
     4081 tacagcacca cgtccggcag ccgtcactcc ggggcactgt tcagagcgga tattttcagt
     4141 gatctgcaga agtccggcac ttccggccag aaacagctga ataccttcat ttgtggcgcg
     4201 gcgataaagc ggtgtgactg caccgctggc cagtcgttta atctcagcga tctgtgccgg
     4261 ctcaccagga aaatacatac gattcgagca ccagtttggc atgggaattc tcctctcaca
     4321 tgcggcctcc atcaggccgt gccgcacctg taaccgggcg accgcatacg aatgcaggtg
     4381 cggccccgaa gccgcagcgc gtggaggagg aatgacgaaa cgtgaccggg tgaggaggcc
     4441 ggcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctggtt
     4501 aaatagtatc cgtctcagtt cgtggcgcaa aatgagaaac caataagaac tcaaaatgaa
     4561 ccacaacaag aacataaaga gactctaaaa gagcaacacc gtttgacata gactcaaaat
     4621 gagtcacaat aagaaacaat aaaaaactca aaaagatgca ccgacataaa ggatgctcaa
     4681 tttgagataa acacaaactc aaaacgaggc aaaaagaatg ctaaaagtat catgcgatga
     4741 tggttcaact aacgtaaaac tggcctggct tgaagatggc gaggtaagaa ccagtctgtc
     4801 aggaaacagc tttaaagaag gctggaaccc gggactgttt aatgccggca aagtttacaa
     4861 ttacgtcgtg gatgaaaaaa aatacaccta tgatttggga agcactgctg tcataggtac
     4921 aacacatgtc agttatcaat acagcaccac caatctgctt gccatacatc atgcactgct
     4981 gacatccggc cttcagccac aggatgtaga actgacagta acattacctg tgaccgaatt
     5041 ctttgataat gacaatcaac caaatgaaga aagaatagaa agaaaaaagg caaatgttct
     5101 ccgtgagata tctctgaata aaggggaaac atttaaaatt aaaaaagtta atgtaatgcc
     5161 tgaatctttg ccagctgcat ttgaatcact aaaaaaagat aaagtaaaca aacttgaacg
     5221 cagcctgatt atcgatcttg gaggaaccac tctggattgt ggtttaattc tcggtgcatt
     5281 tgaaggcatt tctgaaatcc gtggctattc agaaatcggg acatcccgca tcactcatac
     5341 cgtcatgaat gcgctgacga aagcctccac accatgtaat tattttattg ctgatgagtt
     5401 aataaaaaac agacacgata atgaatatct ccagacatta ataaatgatg tagctgaaat
     5461 aaaaaacatt tctcatgtaa tagacagaga agtgaaatct ctggcagaaa gcatcaggca
     5521 ggaaatttca acattctcag gaatgaaccg aatctatctc actggcggag gagccgagct
     5581 tatttaccct cacataaagc aatatttccc gaatctgaaa gtgaacaagg tggatgaacc
     5641 tcagtttgca ttggtgaaag caatggtgca tgcgtaatgg aaagcagtga tccaaaaaaa
     5701 agaaagaaag ttgtggcata cctgcacccg gcactttatc cgcaggacaa cctgacacaa
     5761 caaacaatag actctctccc agtacaaatg cggggagatt tctacagaca atcccttatt
     5821 tgcggagcag ctctttattc tgttgccccc cgactgttaa cgctaatcag cgtttttttc
     5881 agtgagaaaa tcactgcaga aaatctggtg aaacttatag aacaaacaac aggatacaca
     5941 tccacatcaa ttgatataag tgtgttaaaa aacatcatag aggcatcttc agaaaacaaa
     6001 tcagagagca tcacttcaaa agatgatttc gaagaacaaa ccagacgcaa tctttctatg
     6061 ttaaaaaaat aataaaagaa gagcaggttc agtcaattac ctgctcttct tttatacata
     6121 ctaaaaaatc agatttttac gaatattcag attatcataa aaaatgcaac agacaggaat
     6181 gcaataattt tgcattatct cgttgcttgt aatgcaaata tgcattattg tcagtgtgtg
     6241 acaataacag agagatgacg atggcgacca ttaacgcacg tattgatgat gatataaaga
     6301 accaagcaga tgaagtgctg aagctgatga atatcagtca aactcaggct attgcagctt
     6361 tttatcagta tattactgaa caaaagaaac tgccatttgt gatcacctca attgtaaaaa
     6421 cgccgcatga tttattacgt gaaagtacag acatgttagc tgaagctctt gcagtgattt
     6481 ccaatctaca ggtgtggact gaacagcagg atgggattgg aaaagcaaag ctaatggagt
     6541 attaccgccg cctcgacgca ctgtactgtt gtgcaaaaga gaaaataggt cttctttcag
     6601 ataaccggga tgctgaacta ggctgtgtcc cttaatcatc tgagttatag taactgtctg
     6661 tttacacaac atattgaact atggctcgct acgaccttcc cgatgaggca tggaccatca
     6721 tcaagcccct gttacctcct gaacccgcca caccacgggc cggacgccca tgggctgagc
     6781 accgtaaaat catcaatggc atgttctggg tgttatgttc cagtgcacca tggcgcgatt
     6841 tacccgaacg atatggggca tggaaaactg tttataaccg ctttaaccga tggtcaaagt
     6901 caggcgtgat taatattatt ttcaacaggt tgctttcgct acttgatgca aacggcttta
     6961 ttgactggtc tgccaccgcg ctggatggca gtaatatccg ggcgctgaaa tgtgccgccg
     7021 gtgctcaaaa aaacatcccg atatcgacgg agataatggg ccgggtcgct ctcgcggcgg
     7081 ttttggcacc aaaatccatc tggcaacaga cggaagtggc ctcccgttaa atatcgtgct
     7141 aagccccgga caagctcatg aaagccagtt cgcgcaacga cttctggacg ggattggcgt
     7201 tcagcgtcag aacggcagca tgaaacgccg tggacatgcg gtactggctg ataaagcgta
//
LOCUS       DAJTYL010000160         7260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYL010000160 DAJTYL010000000
VERSION     DAJTYL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.160"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1310
                     /locus_tag="OO572_003607"
     CDS             <1..1310
                     /locus_tag="OO572_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6662463.1"
                     /translation="EGSVWPKSIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCGE
                     EQYLKFGDDATSFGLKWEKGKPETVYYLCEHNGCVIRQSELDQTGGRWICDNTGMWTC
                     DGLTFFSASGNEIPPPRSITFHIWTAYSPFTTRVQIVCDWLDALKDPNGVKTFVNTTL
                     GETWEEAVGEKLDHQVLMDKVVPYTATVPVRVVYLTAGIDSQRNRFEMYVWGWAPGEE
                     AFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDIGGIDGEIVYQR
                     SKKHGIFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTP
                     VNEATSYAIRFPDNPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLV
                     YAYAALRVSVQRWQLDLAVQLHPARMQLHNRPLKNWQRSCPEE"
     gene            1311..1514
                     /locus_tag="OO572_003608"
     CDS             1311..1514
                     /locus_tag="OO572_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662464.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            1511..3104
                     /locus_tag="OO572_003609"
                     /pseudo
     CDS             1511..3104
                     /locus_tag="OO572_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3094..4512
                     /locus_tag="OO572_003610"
     CDS             3094..4512
                     /locus_tag="OO572_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6662465.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPLAAMPEMTVEKARPILAASPQANAGPSLRDQIMAL
                     DEAKGAEAQAEKLAACPGMTVENARAVLAAASGRAEPVSASTTAMFEHFMANHSPAAV
                     QGGVPQTSADGDADVKMLMAMP"
     gene            4549..4896
                     /locus_tag="OO572_003611"
     CDS             4549..4896
                     /locus_tag="OO572_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6662466.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            4954..5982
                     /locus_tag="OO572_003612"
     CDS             4954..5982
                     /locus_tag="OO572_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6662467.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6034..6420
                     /locus_tag="OO572_003613"
     CDS             6034..6420
                     /locus_tag="OO572_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6662468.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            6432..6809
                     /locus_tag="OO572_003614"
     CDS             6432..6809
                     /locus_tag="OO572_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662469.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            6796..>7260
                     /locus_tag="OO572_003615"
     CDS             6796..>7260
                     /locus_tag="OO572_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662470.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1649 a         1723 c         2292 g         1596 t
ORIGIN      
        1 ttgaaggttc ggtatggcca aaatccatac gcggttcaac gccaaaaatt aaaggctcct
       61 gtcagattga aaaagctgcg aatgaatctg cgcatttcat gcggttttat gtcccttgcc
      121 ctcattgcgg ggaggagcag tatctgaagt ttggcgatga tgcgacgtcg tttggcctga
      181 aatgggagaa gggtaaaccg gaaacggtgt attacctgtg tgaacataat ggctgcgtga
      241 tccgtcagtc tgaacttgat cagaccgggg ggcgctggat ttgtgacaat accgggatgt
      301 ggacgtgtga cggtctgacg tttttcagtg cttcgggtaa tgaaattccg ccgccgcgct
      361 ccatcacttt ccatatatgg acggcgtaca gtccgttcac caccagggta cagatagtct
      421 gtgactggct ggatgcactg aaagatccca acggcgtgaa aacttttgtg aacaccacgc
      481 tgggcgagac ctgggaagag gccgtgggcg aaaaactcga tcaccaggta ctgatggata
      541 aggttgtgcc ttacacggca acggtgcctg tccgggtggt ttatctgacg gcgggcattg
      601 actcgcagcg aaaccgtttt gagatgtatg tctgggggtg ggctccggga gaggaagcct
      661 ttctggtgga taaaatcatc atcatgggac gtcctgatga ggaagagacg ctgttacgtg
      721 tggatgcggc gatcaataaa aaataccgcc atgcagacgg taccgaaatg accatttccc
      781 gtgtctgctg ggatatcggg gggatcgatg gcgaaatcgt gtatcagagg tcaaaaaaac
      841 acggtatttt ccgggtactg ccggtaaaag gtgcctctgt ctatggcaag ccggtgatca
      901 ccatgccgaa aacccgcaat cagcggggtg tgtatctgtg tgaggtgggg acggataccg
      961 caaaagaaat tctctatgcc cgaatgaagg ccgatcccac ccctgtgaat gaggccacat
     1021 cgtatgccat ccgctttcct gataatccgg agattttttc gcagacagag gcacaacaac
     1081 tggtggcgga ggaactggtg gaaaagtggg aaaaaggaaa gatgcgtctg ttgtgggata
     1141 acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctatgcg gcattacgtg
     1201 tgtccgtgca acgctggcaa cttgatctgg ctgtgcagtt gcatcccgca aggatgcaac
     1261 tgcacaaccg acccttgaag aactggcagc gaagctgtcc ggaggagtga atggttacag
     1321 tcgctgaact gcaggcgctg cgtcaggcgc gccttgattt attaaccggt aaacgggttg
     1381 tgtcggtaca gaaagacggt cgcaggatcg aatatacggc agcgtccctg aatgagctta
     1441 accgtgcgat caacgatgcg gagtcggttc tgggtacgac ccgacgccgc cgaccgctgg
     1501 gagtgaggtt atgaaacgaa cgcctgtcct gattgatgtg aacggcgttc cgcttcgtga
     1561 gagtctcagc tacaacggag gcggcgcagg atttggcggg caaatggcag agtggttacc
     1621 accggcgcag agtgccgatg cggccctgtt gcctgcattg cgtctgggga atgcccgggc
     1681 agatgatctg gtgcgcaata acggtatcgc ggctaatgcg gtggccctgc ataaggatca
     1741 tatcgtcggg cacatgtttc ttatcagcta ccgtctgaac tggcgttggc tggggatgcg
     1801 ggagaccacg gcaaaaagtt ttgtcgggga ggtggaagca gcctggtcgg aatacgcaga
     1861 aggaatgttt ggcgagatcg acgtggaagg gaaacgcacg tttacggaat ttatccgtga
     1921 aggggtgggc gtgcatgcct ttaacggcga aatctttgtg cagccggtct gggatacgga
     1981 aaccacgcag ttattccgta cgcgttttaa agccgtgagt ccgaaacggg tggacacgcc
     2041 aggacacggt atggggaacc actttctgcg ggccggggtt gaggtcgatc ggtatgggcg
     2101 ggcgattgcg taccatatct gtgaggatga ttttccgttc tctggtgccg gacgctggga
     2161 gcggatcccg cgtgagcttc ccaccgggcg tccggccatg ctgcatattt ttgagccggt
     2221 ggaggacggg cagacccgtg gggctaatca gttttacagc gtaatggagc ggatgaagat
     2281 gctggactcc ctgcaggcca cgcagcttca gtcggccatt gtgaaggcga tgtatgcagc
     2341 cacaatcgaa agtgaacttg ataccgaaaa ggcatttgaa tatatcgcgg gggcaccgca
     2401 aggtcagcag gataatccgc ttattaatat tctggagaag ttcacccgct ggtatgacac
     2461 gaataacgtg acgctgggcg gtgttaaaat tccgcacctt ttttcctggc gatgcgctga
     2521 acctgcagac agcgcaggat tcagacaatg gattttcggc gcttgaacag gcgctgctgc
     2581 ggtatattgc agccggtctt ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg
     2641 tcagttactc aagtgcccgc gcctccgcca atgagtcgtg gcgctatttt atggggcggc
     2701 gaaaatttat tgcggcccgg ctggccacgc agatgttttc ctgctggctg gaagaggcgc
     2761 ttcttcgggg gattattcgt ccgccacggg cgcgttttga tttttaccag gcgcgttcag
     2821 cctggtcacg ggcagagtgg attagctccg gaagaatggc cattgacggg ctcaaggagg
     2881 tccaggaatc ggtgatgcgc attgaggccg gactgagcac gtatgagaaa gaactggcac
     2941 tgatgggcga agattatcag gacattttcc gccagcaggt cagggaatcc gcagagcgcg
     3001 aaaaagccgg actctcacgt ccggtgtgga tagcccaggc gtatcagcag cagatagcgg
     3061 agagtcgcag gccggaagag gagacaacat cacgtgagac gtaatctttc acatattatc
     3121 gctgcggcat tcaatgagcc gctgcttctg gagcccgcct atgcgcgggt tttcttttgc
     3181 gcgctcgggc gtgagatggg cgcagcaagc ctttcggtac cgcaacagca ggtacagctt
     3241 gacgctcccg ggatgctggc tgaaacggac gagtacatgg ccggaggcaa acgacctgcc
     3301 cgtgtttacc gggtggtgaa cggtattgct gtactgccgg tgaccggcac gctggtacac
     3361 cggctgggtg gcatgcggcc attttccgga atgactggct atgacggcat tgtcgcctgt
     3421 cttcagcagg caatggcgga cactcaggta cggggcgtac tgctggacat tgacagttct
     3481 ggcgggcagg ccaccggcgc gtttgactgc gctgacatga tttaccgtct ccggcagctg
     3541 aagcctgtct gggcactgtg caatgacacg gcctgttcag ccgccatgtt actggcatcg
     3601 gcctgctccc gacggctggt tacccagaca tcccgtatcg gctccattgg ggtgatgatg
     3661 ggccatgtca gctatgccgg tcatctggca caggccggtg tggatatcac gctgatttac
     3721 gccggatcgc acaaggtgga tggcaatcag tttgaatccc tgccagcaaa ggttcgccag
     3781 gacatgcagc agcgcattga tgcggcgcgc cggatgtttg ctgaaaaagt ggcgatgtat
     3841 accggtttat ctgtggatgc ggttatggga acagaggccg ccgtttttga aggtcagtcc
     3901 ggtattgagg ccgaactggc ggatgaatta atcaatgcgt cggacgccat cagcgtgatg
     3961 gctgcgacgc tgaacacaca cgatacagga ggcactatgc cgcaattaac tgcaacggat
     4021 gctgccgcgc aggagaacca gcgagtgatg gggatcctga cgtgtcagga agcgaaagga
     4081 cgtgaacaac tggccacgat gctggcaggg caacagggta tgagcgttga gcaggcccgg
     4141 gcgattctgg ccgtggcggc accgctggcg gcgatgccgg agatgacggt ggaaaaagcc
     4201 cgtccgattc tggctgcttc accgcaggcg aatgccggac cctcacttcg tgatcagatt
     4261 atggccctgg atgaggcaaa aggggcagag gcgcaggctg aaaaactggc ggcctgtccg
     4321 gggatgaccg tggagaatgc ccgggctgta ctggcggcag cctcaggcag ggcagaaccg
     4381 gtttctgcat ccacaaccgc catgtttgaa catttcatgg cgaaccattc accggcagcg
     4441 gtacagggtg gcgtgccaca gacgtcagca gacggtgatg cggacgtgaa aatgctcatg
     4501 gccatgccat gaagtcagtg ctgaccatca atatgaggtt tttacaatat ggtaacgaaa
     4561 accatcactg aacagcgtgc ggaagtacgt atttttgccg gtaatgatcc ggcccatacc
     4621 gccacaggct gcagcgggat ttcctcggca acaccggctc tgacgcccct gatgctggat
     4681 gaggataccg ggaaactggt ggtctgggac ggacagaagg caggccatgt ggttggcata
     4741 ctggtactgc cgcttgaagg tacagagacg gtgctgacgt attacaagtc ggggaccttt
     4801 gcgacggagg ccatccactg gcctgagaga gtggatacac acaaaaaggc gaatgccttt
     4861 gccggcagtg ccctgagtca cgcagcgctg ccgtaacatt ttatcaggtc actgcggtag
     4921 cctgactgat ttctgaatga aaggaactga tttatgggat tgtttacgac ccgccagtta
     4981 ctcggttata ccgaacaaaa agttaaattt cgtgcgctgt ttctggagct gtttttccgc
     5041 cgtacggtga atttccatac cgaagaggta atgctggaca aaattaccgg aaaaacgccg
     5101 gtggcggcct atgtctcccc ggttgttgaa ggaaaggtgt tgcgtcaccg tggcggtgaa
     5161 actcgtgtac tgcgcccggg ctacgttaag ccgaaacacg aatttaatta ccagcaggcg
     5221 gtggagcgtc ttcccggtga agatccggcc cagctgaacg gcccggctta ccgtcgcctg
     5281 cgtatcatca ccgataacct caaacaggaa gagcacgcca ttgtccaggt ggaagagatg
     5341 caggcggtga atgccgtgct gtatggcaaa tacaccatgg aaggagagca gtttgaaact
     5401 gttgaggtcg attttggccg gtcggtggga aataacatta cgcaggcagg agggacggag
     5461 tggagcgctc aggaccgcga tacctttgat ccgacgcatg atctggatgc gtactgtgat
     5521 tttgcttccg gcaccatcaa tattgccatt atggatggta aggtctggcg tctgctgaat
     5581 ggctttaagc tgttccgcga aaaactggat acccgtcgtg gctcaaattc acagctggaa
     5641 acggcagtga aagacctggg ggcggtggtg tccttcaaag ggtattatgg tgatctggca
     5701 atcgtggtgg caaaaacgtc ctatgtggca gaggacggta ccgaaaaacg ttatctgccg
     5761 gacggcacac tggtactggg aaatacggcc gcagagggta ttcgttgcta cggtgccatt
     5821 caggatgcgc aggcattgtc tgaaggcgtg gtggcgtctt cccgttatcc gaaacactgg
     5881 ctgaccgtgg gtgatccggc gcgtgaattc accatgacgc agtccgcgcc gctgatggtg
     5941 ctgccggatc cggatgagtt tgtggtggtg caggtgaaat aatccgtgag cgggagctaa
     6001 atgctcccgt ttttcattca atcaggagcg ggtatggcta cgaaagaaga aaacctgaaa
     6061 cgtctttgtg agctggctga gaggctgggg cgtgaaccgg atgtctccgg tagtgcggca
     6121 gatattgcgc agcgagtggc ggaactggag gaagagcttg gagatgcggg ggaggtcgca
     6181 gagccggata cgtactcacc atcgaaagat gatccgaccg cgcataaagg cgagtcagtt
     6241 cctgaacagc caggcattgt taccggagac gaaacggggc aggttacggt ggtggcactg
     6301 gcgacactgc atacaaaatg taatggttca gtgatatttg tttcaccggg aacgtcgttt
     6361 cgtgtttctg ctgaggtggc tgccagcatg gcggcaggtg gtctggcaaa aaggcagtaa
     6421 caggagatgt cgtggacggt ttcgataatc tgtttgatgc tgcgcttgcc ggtgtcaatg
     6481 aagtcattct ccgtgatatg gggatcagcg ccgtcattac gtccggagaa cttgagggga
     6541 cacacctgac aggggttttt gatgatcccg aaagtatttc ttttgttgcc ggggggatcc
     6601 ggcttgagga ttcctcaccg tgcctgtttg tgaaaacggc agatatatcg cagttacgcc
     6661 gtcaggatac gttaactatc ggggatgatt ctttctttgt ggatcgtatt actccggatg
     6721 acgggggatg ctgttatatc cggttaaggc ggggcagtcc tgccccacag aataatgcca
     6781 gaatgaggcg atatgatgaa gggacttgag aatgccatcc gcaatctgaa cagcctcgat
     6841 acccgcatgg tgccacagac cagcgcatgg gcggtaaacc gtgtggcggc aaagattgtt
     6901 tctgtcgcca cacggcaggt tgcgcagaat accgttgccg gggataacca ggtgaaaggt
     6961 attcccctga aaacggtcag ggagcgtgtc aggctgctta aagccagccc ctcaggaaaa
     7021 atgtatgccc gcatgcgtgt taaccggggt aacctgcccg ccattaagct gggtaccgca
     7081 caggtcagac tggcccgctc ccggcatggc agcaactcac gtcatcgtgg cagcgtactg
     7141 aaagtgggga aatacctttt ccgggatgcg tttattcagc aactggcgaa tggtcgctgg
     7201 catgtgatgc ggcgtattga cggcaaaaat cgttacccca ttgatgtggt gaaaatcccg
//
LOCUS       DAJTYL010000161         7242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYL010000161 DAJTYL010000000
VERSION     DAJTYL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.161"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(69..762)
                     /gene="ospZ"
                     /locus_tag="OO572_003616"
                     /pseudo
     CDS             complement(69..762)
                     /gene="ospZ"
                     /locus_tag="OO572_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            818..970
                     /locus_tag="OO572_003617"
                     /pseudo
     CDS             818..970
                     /locus_tag="OO572_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2351..3298
                     /gene="icsP"
                     /locus_tag="OO572_003618"
     CDS             2351..3298
                     /gene="icsP"
                     /locus_tag="OO572_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6662471.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3860..4232)
                     /locus_tag="OO572_003619"
                     /pseudo
     CDS             complement(3860..4232)
                     /locus_tag="OO572_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4476..4595
                     /locus_tag="OO572_003620"
                     /pseudo
     CDS             4476..4595
                     /locus_tag="OO572_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4845..5711
                     /gene="ospB"
                     /locus_tag="OO572_003621"
     CDS             4845..5711
                     /gene="ospB"
                     /locus_tag="OO572_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6662472.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            6040..6780
                     /gene="phoN2"
                     /locus_tag="OO572_003622"
     CDS             6040..6780
                     /gene="phoN2"
                     /locus_tag="OO572_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6662473.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7140..>7242)
                     /locus_tag="OO572_003623"
     CDS             complement(7140..>7242)
                     /locus_tag="OO572_003623"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6662474.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2267 a         1369 c         1395 g         2211 t
ORIGIN      
        1 aatcgatact ggaaaaactc taactcggtg gccagttttt gttgaccact tcatacaatt
       61 aatatacatt aagtaacagg cattcgagcc ttgatttgag aaagcctgtc attcatttgt
      121 tttatgtagc tatctatttc ctctgatgca taactaaaaa gttctcccat ttcacgagca
      181 ataataatct cagatttaat agactttaat ctctggcgac tcatctctta acacttgcat
      241 aatctctctt tttatatcat atagttctat aaaccgattt gcccgtagtt ctccttggga
      301 agaatcattt ctgaatacaa caattatata caaaaaatca ctattttctt tagagaagat
      361 cttacaacta atctctggga gctcacataa tgcatgtaag tcaggatggt acaaactatc
      421 cacagaaaat tgccccatag gacgttttcc agatataact gtcaaccagc ttccttccgg
      481 tgtactggct tcggctctgg aaataggctg aaaatcttcc ggacatggag aatacattgt
      541 cttcatataa ttaagtcgtc tggatttctc attaataaac tcctctattt ttaattgtgc
      601 catacttctg gttataccag agatatactt cttgtcatat atgtttggtg attcattaaa
      661 gtaaccatgt tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt
      721 tattggaaat acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa
      781 atataaacat taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca
      841 ctttatctat cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcaattacaa
      901 tcgctggcga cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa
      961 cctcgcttag gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg
     1021 cgtccatatt atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag
     1081 aaaatttata ccctgcaggt atcataaaca tgcttgataa cttaattggg gctccctctt
     1141 ccttttatgg tgtaattgtt tgattgaata cttccgggga tttcagtatg aaatgaagta
     1201 tatttaatat actttacaaa aagtttgtat tagtggaata aggacaatag cttatccaaa
     1261 ctgccaagcc tctttattat aagtaagatc tggcactgga atattctcaa aaggactaac
     1321 atcatcacca cgaccttcaa ccaaccattt tattgcattt aaatcccaga atagttcagt
     1381 agtggtcaat ttgggaaacg cacaatattg tacggttacg ttatcgcaat agagtgaact
     1441 ctcgccctat acggcgtttt caaggattag tccttaatcg gacaaccaca ttaaagcata
     1501 ataaataatt aagctttaat aatatactat agatacagtt ctattataaa aaatactatt
     1561 ttaggtgatg cattaaatta gtagaatatt tattctttta taaaagcacc aatgaattgt
     1621 ccaagtaaac atgagataaa atgtgtagag gataactgag tttggtcact ttaactgtat
     1681 tagtcgcagt ctgactctcg actttaaaag gatgggaatt acctgtactg atagtgagaa
     1741 agctttccat tgaaataagt caaataaaag ttgatggctt cagtgctaaa gtgaggtgcc
     1801 cgctagccgt attcactcga atcggaattc aatgtattgc ttctgccatt tccataatat
     1861 ttcacgggaa aaacaaaatc tgaaaaacta tacttaagtg ttgtattgag tcagttgaga
     1921 tacaaataca tatttatcag cacacgttac gtcaaataca ggaggttgtc atgttggtac
     1981 tgaaaggcac gttggctatt gcagccgtga tgacaattta ttgtcttatc gttgttctta
     2041 tggaacgctt ttctgattga tttcatattg gcgagagaac aagaaggtga gtagaattgt
     2101 cgcggaatcc tgaaactatc agcctcaggt atgaacacca aaaggtaggt agagaaatct
     2161 atggcccccg ttactttatt cgcacaatga ggtatttttt tatttttaca aaagttcaat
     2221 aaatattata ataaaagata tgttcttggt aacataaaca aatatttaat taaaataatg
     2281 cttggcattg acgtttatct tataaagtaa gaagatcatg gacatttcaa ccaaaaaagt
     2341 agagttctcg atgaaattaa aattctttgt acttgcactt tgtgtacctg cgatctttac
     2401 tacacatgct accactaact atccactttt cataccggac aacatcagta cggatattag
     2461 tctaggatct ctgagtggca aaacaaaaga acgcgtttat catcccaagg aaggggggag
     2521 aaaaattagt caactggact ggaaatacag taatgcgact attgttagag gtggcatcga
     2581 ttggaagcta attccaaaag tgtctttcgg agtttccggt tggactactt taggtaacca
     2641 gaaagcaagc atggttgata aagactggaa caattccaat acccctcagg tatggaccga
     2701 ccagagctgg catcccaata cgcatctccg tgatgctaac gaattcgagc tgaatcttaa
     2761 aggttggtta ttaaataatt tggattatcg acttggacta atagcaggtt accaagagag
     2821 tcgttacagt tttaatgcaa tgggagggag ttatatttat agtgaaaacg gggggagcag
     2881 gaataaaaaa ggggcacatc ctagtggtga aagaacaata ggttacaaac agctcttcaa
     2941 aataccttat attggattaa ctgctaatta ccgccatgag aattttgagt ttggagcaga
     3001 actgaaatat agtggttggg ttctttcatc tgatacagat aaacactatc agactgagac
     3061 aatttttaaa gatgaaataa aaaaccaaaa ttactgctct gttgctgcga atattggata
     3121 ctatgtcacc cccagtgcaa aattttatat agaaggctcc agaaattaca tttctaataa
     3181 aaaaggtgat acatctcttt atgagcaaag taccaatata tctggcacca ttaaaaatag
     3241 tgcaagtatt gaatatattg gttttctcac ttccgcaggt ataaagtata ttttttgaat
     3301 tggttcaccg agttgattac gttttcatgg ctaaaaattg ccgcccattg tgagtcatat
     3361 tcgccctgac tttccagaac catacgaact gcccgttgac ggacttcggg ggaaaaacgt
     3421 gtatttttag tcattctgtt tacctctttc tcagggcgtt tagtctccag gatttccggg
     3481 gcggttcaaa aaactcttcg ttgattactg tggacttact gttggcatta ccgaccctga
     3541 gaccggagaa ataagaaccg cttagatcat cgtggctgtt ctcggggtat caagttacac
     3601 atgggcagag gctacccaag gtctactatt atcgcctgaa agcgctgatg gaacagtgct
     3661 atcagggaca tgctgatgaa aatacctaaa taatcagcca gcactctatc tttccaaatc
     3721 cacagcatag caaagagagc aaaagagcct gtaaattcag aaattaagga aacgcctctt
     3781 ggtgtacata atctatctta tgttaaatga aatctaattt cttgattata tgctggatcg
     3841 ttcgtgggtg gctttgcccc tatatttcca gacacctgtt atcattggcc cgctgccgca
     3901 gatattcccg tggcgagcga taatccagcg cactatgcgg atgccattcg ttataatgct
     3961 cgaatgcctc tgcaaggttc tttgctgccg ttaacccgtc aggtttgggc atgatactga
     4021 tgtagtcaca ctttattgtt ttcacgaagc tctttgctat tctgttactc tccggactcc
     4081 gcaccgccgt gttcttcggt tcaacaaagt cccaacatcc gggtgaactg gcgtgtttca
     4141 ttagctcggt agcatgaacc attatccgtc agtcacttca ctggagacgc cggaagctcg
     4201 ttgccgaagc ggtgttccac cgctcccagc atgtcgccaa cgggagagca tctacggtct
     4261 gattgaactg tttattcaaa ttgtccaccg tctgtcagta cgctctgaac gtcggctggt
     4321 taaaacactg ctcgctgatt ttcagagggt tcacggtaaa acagcacttt tattccggat
     4381 tgcggaagcg gctctgaata atccggatgg gctggttaaa gaggttgtgt accatctgca
     4441 tattgttgaa ccagatcgca gcggtaagag aagttcatca tacttggcgc agttacgcga
     4501 tgtttctgct agaggtgatg ccgtaaagaa cggcagaaca ttaccggaac aagatagcgg
     4561 tctacctgca ctggtatcag acccggggct tccccggatg atctccacgg tactttaggt
     4621 actgtgtcta tattatgtag gtaggatcaa ttaactttgt agttgaaatt ctttcattga
     4681 aataatgaat aaaaaagaca aaccgcatta aacaatgcca gaatataatt gcatttttgt
     4741 tccgtttttt taaatcatag tatacaaaga agcttgcata ctatatgaaa atataacacc
     4801 ctttactatg atgtttataa aacaatatat ggagtcatgt aggtatgaat ttagatggtg
     4861 ttagaccata ctgtagaata gtcaataaaa agaatgaaag catatcagat attgcatttg
     4921 cacatataat aaaaagggta aaaaattcat catgtactca cccaaaagca gcattggttt
     4981 ttttaggaga gaaaggtttt tgtgatagca atgatgttct atctattatg ggacaacaaa
     5041 taccaagagt atttaagaac aagatgttat atgattatgt ttttaaaaat gaaaaaagta
     5101 aaaatgattt tctaaaaatg gctgaatcat ggctaccaca gagtgaacca atagtaataa
     5161 ataatgatga tgacgcattg aatgctgctg cttatttttc tgtaaaaaaa gcgaaaataa
     5221 aaacagtaaa cgatactgat tttaaagagt ataataaggt ttatattctt gggcacggta
     5281 gtcctggttc tcatcaatta ggccttggtt cggaacttat tgatgtacaa acaatcattt
     5341 caagaatgaa agactgtggt attctaaatg tgaaagatat ccgttttact tcatgcggct
     5401 ccgctgataa agtggctcct aaaaatttta acaatgcccc tgctgaaagt ctttcttgta
     5461 tccttaactc tctgcctttt tttaaggaaa aagaatcttt gctagagcag ataaaaaaac
     5521 accttgaaaa cgatgagtca ttgagtgatg gtctaaaaat atccggctat catggatatg
     5581 gagttcacta tggtcaagag ctttttccct actcacatta tcgttcaact tcaattcctg
     5641 ctgatccgga gcatacagta aaaagaagct ctcagaaaaa gacttttatt attaataaag
     5701 aactggatta gtataaaatt tttaacctat agttaattgt ttgcatttaa gatgaagtta
     5761 ttttatcttt agtagaaaag tgcttcccta tgagggaaaa tcacaaatca tcataataca
     5821 gagacaaaac gatacgaaaa aatagataaa aaacatcatt cttttaccac attattttcc
     5881 gtgaatatga aaaataatgt tattacttta atataagact attttttgtt tttccatcac
     5941 tctgttcaaa tttttccgca tgacttgtgt tttttgtaat acagctcgtt ttttacagct
     6001 gaccaaaatc atcaattaat tatgctaagg aaataaatta tgaaaaccaa aaactttctt
     6061 cttttttgta ttgctacaaa tatgattttt atcccctcag caaatgctct gaaggcagaa
     6121 ggttttctca ctcaacaaac ttcaccagac agtttgtcaa tacttccgcc gcctccggca
     6181 gagaattcag tagtatttca ggctgacaaa gctcattatg aattcggccg ctcgctccgg
     6241 gatgctaatc gtgtacgtct cgctagcgaa gatgcatact acgagaattt tggtcttgca
     6301 ttttcagatg cttatggcat ggatatttca agggaaaata ccccaatctt atatcagttg
     6361 ttaacacaag tactacagga tagccatgat tacgccgtgc gtaacgccaa agaatattat
     6421 aaaagagttc gtccattcgt tatttataaa gacgcaacct gtacacctga taaagatgag
     6481 aaaatggcta tcactggctc ttatccctct ggtcatgcat cctttggttg ggcagtagca
     6541 ctgatacttg cggagattaa tcctcaacgt aaagcggaaa tacttcgacg tggatatgag
     6601 tttggagaaa gtcgggtcat ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt
     6661 ttaatgggag catcggttgt tgcagtactt cataatacac ctgaatttac caaaagcctt
     6721 agcgaagcca aaaaagagtt tgaagaatta aatactccta ccaatgaact gaccccataa
     6781 agctggacag cctgtatcag gctatggagg gcccatagac aaatctaccc tatatgagca
     6841 taggaggagt ctatgggcac accacgtttt acccctgaat ttaagggatt actggaaagg
     6901 ctgggacata tccctccggc agaagcagaa aaagcttatt atgctgccat cggaaacgat
     6961 gatctggcaa cctgagttca cagataaaac attctctagg aaactcgggg cggttccgtt
     7021 caccacatgc aatgtggtgt tgcaggggaa cggtctgcac catcccctat gtcgatcaat
     7081 ataacagaaa tgacaacttc agattcaggg cacaacctaa atatatttta ggtcacctct
     7141 caaatcgttt gcctgatacc gctccatttt ttaataaaaa aatcaatcat ttttgaatcc
     7201 ttgcttttca ttgcattgtc aagcatagtg tccccactat tt
//
LOCUS       DAJTYL010000162         7113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYL010000162 DAJTYL010000000
VERSION     DAJTYL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.162"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(2..94)
                     /locus_tag="OO572_003624"
     tRNA            complement(2..94)
                     /locus_tag="OO572_003624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO572_003625"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO572_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6662475.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO572_003626"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO572_003626"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6662476.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO572_003627"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO572_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6662477.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO572_003628"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO572_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6662478.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO572_003629"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO572_003629"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6662479.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..>7113)
                     /gene="mltB"
                     /locus_tag="OO572_003630"
     CDS             complement(6114..>7113)
                     /gene="mltB"
                     /locus_tag="OO572_003630"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6662480.1"
                     /translation="TTTGTPSGGFLLEPQHNVMQMGGDFANNPNAQQFIDKMVNKHGF
                     DRQQLQEILSQAKRLDSVLRLMDNQAPTTSVKPPSGPNGAWLRYRKKFITPDNVQNGV
                     VFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVMGKTRILDALATLSFNYPRRA
                     EYFSGELETFLLMARDEQDDPLNLKGSFAGAMGYGQFMPSSYKQYAVDFSGDGHINLW
                     DPVDAIGSVANYFKAHGWVKGDQVAIMANGQAPGLPNGFKTKYSISQLAAAGLTPQQP
                     LGNHQQASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1639 a         2009 c         1718 g         1747 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtt
//
LOCUS       DAJTYL010000163         7069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYL010000163 DAJTYL010000000
VERSION     DAJTYL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.163"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..154
                     /locus_tag="OO572_003631"
     CDS             <1..154
                     /locus_tag="OO572_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6662481.1"
                     /translation="NPVRGYPSMLVSILFLGGVQLIGIGVLGEYIGRIYLETKSRPRY
                     LIKSRK"
     gene            167..1432
                     /locus_tag="OO572_003632"
     CDS             167..1432
                     /locus_tag="OO572_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6662482.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1499..2122)
                     /locus_tag="OO572_003633"
     CDS             complement(1499..2122)
                     /locus_tag="OO572_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662483.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2873..3211)
                     /locus_tag="OO572_003634"
                     /pseudo
     CDS             complement(2873..3211)
                     /locus_tag="OO572_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            3276..3734
                     /locus_tag="OO572_003635"
     CDS             3276..3734
                     /locus_tag="OO572_003635"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013043.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6662484.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3866..4126
                     /locus_tag="OO572_003636"
     CDS             3866..4126
                     /locus_tag="OO572_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6662485.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(4216..6213)
                     /locus_tag="OO572_003637"
     CDS             complement(4216..6213)
                     /locus_tag="OO572_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6662486.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(6213..>7069)
                     /locus_tag="OO572_003638"
     CDS             complement(6213..>7069)
                     /locus_tag="OO572_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6662487.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1889 a         1628 c         1487 g         2065 t
ORIGIN      
        1 taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg gtggagtgca
       61 actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag aaacaaaatc
      121 aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga agatattcat
      181 aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga tttggcttag
      241 acgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt tttggtataa
      301 agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg gttattttca
      361 aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt acgcaccatt
      421 attatcaaac ttttttggaa taataattag agctataatt atatggttct tatttaccga
      481 tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt taatttgcat
      541 gcccgggctt gatgaagtcc aagccaatat aacaaatgct cactggtatt tatcattgta
      601 tgttgcaatg ataataatat ctgaagaatc caagtcaaaa ttatggaaag cacatgattt
      661 attttttata gtattatctg gattgagcgg tccatttatt atattcataa ttgcatcatc
      721 tgtttttaaa tatttacact tatcaaaagg agtaataagc ataagaggtt tatacttatt
      781 ttatactcga ctgccatatt tggcaatgat cttgtgtggg ctgatacagg caacatcaat
      841 aattcttaca tttaatggaa caagatcaca tgctccatta ggattcagtt ttgatgttat
      901 gtcatctata atatcatcta atgtatttct tttttcattt gtcccttgga atatcgctaa
      961 tgctggatgg gacaatcacc ttttatcgta cacactatca actttagtaa ttacattatg
     1021 tgttttcatt tatattaaag gaaattggca aatgaaagtg tttgccaccc ttccaatact
     1081 tattgtcgca ttttctatgg caaaacctca actggcagat tctgttcctc aactgccaac
     1141 acttgcaacc ggaaacgggt caaggtattt tgtaaatatt catattgcaa ttttttcatt
     1201 aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc ttaaaaatat tctttaaaat
     1261 ctatgtttca gtcatcctta ttgcaatgat aaaattaaac ttttttataa cccctcttcc
     1321 tgacatgaac tggtctcaag gagcagaatt aataaataaa gcaaaacatg gagaagccgt
     1381 ctcatttaca gtgctccctc catggttaac tcttgattta ataaaaaaat aaaacttcac
     1441 attaataaat aacaatgaat ccgcctccct acgtattccc gtagggaggc tgtcgtgttt
     1501 atagtagcct ggtaatgcta aatgcaaacg aactagagat tcctgctccg ctaagcgtat
     1561 tgcgcagggt caccctaaat gtatctacgc caatataagt aacatcacaa ctaactgaat
     1621 caggcgcaga tataggtgtt atctgcacaa tataaccgct agcaactgct gcgagcggat
     1681 gagaaaagct aacctcatac acacctgtag caaccacgtt aaccgatgaa acaccagtag
     1741 caacgtcgat actaccattg gcgttaattt ttcccgagcg tgtgactgac aatgcagaag
     1801 ccgatgttgc cttactggtt gtttctgtaa aaacacaccc aatgtcaact acctcgcaat
     1861 tagctcctgt tatccagtat ggtcgatctg cacttggctg ataatcccct gcgctctgga
     1921 acgttgtgcc gttgagtatg actgtaactt ttcccccacc tgagcttgtt acttgaatat
     1981 tagtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt tccaccgttt ataaccacag
     2041 ttacagggcg cgtcccagta ttatctatag caaggtctgc ccccccggca ttagcctcaa
     2101 agtatgggtt gtttaatacc aaagcgcacg aaccgttaag gccatttatg gctaattgcg
     2161 caccacctgt actggaatcc ccctgagtac catttccttc acaagtcagg ctattgatag
     2221 ttgtaccagc accccagcgg tccccgagga taccccattc actgttctga ctgcacgtaa
     2281 gcctatttgc agttatagcg ttcagttcag aatgatttac ggagttggta gcacgccaac
     2341 cgatacgatt actctgcaag tacacgttcg tcatgtcgct cgtgagcatc gagtcagtgc
     2401 gtaacccttc cccgtcaggg tggcggcaga taacaatgtt ttcaagacga gtatgtgctt
     2461 tattctgcac cagcataccg taacttccgg gattcccttt cagcgtcatg ttgccgatgt
     2521 agtcatatgc agccaatctc tgactgttaa acgctgcgtc acctagcatt tttacgttaa
     2581 aatcagtagc tctacattcc agtattgtct cagccatgtt ctcgcctgag taaaaacagc
     2641 ggacacccgg agcacctaca aagtcggcag ggtaatcatc ataattaagt accagaggag
     2701 tggtaacatc atacacccct tgctttattt ttaatgttcc accgaaaggc cacgatgtaa
     2761 gcgggtcttg atatatttca ttttttattg caaatagctc atcttgaaga tttttcccgc
     2821 tattagtgcc aatgtaagta gccccttcat cagattgaag taactgcttt aactgatccg
     2881 ggtcatactt cagcaaattc ggaaaataga actgttgcga accatacgca tcatatacag
     2941 ccattgaatg gccttgctca gttacaaatt tggcaatctg cccgttgtac acaggatatc
     3001 cggcagcatt gataacaatt ggctgagaaa cagggacgtg agatccgtct tcattctcta
     3061 cataaacctg aatctggttt tcaggattta ccgggtcagt gtcaattttc ccgatataaa
     3121 tttttccatt ggcatttgct ttaaacgagc gagctaaagt aaataactga cttggttgtg
     3181 aaacaacaac gttgtataat tgttctggca taataaacat tccggagaaa ataatgagta
     3241 agagacgtca cgaggtagta actcgggagc gccttatgga ggttctctat tacaataaag
     3301 aaacaggaat tttcacttgg aaaaagaaat taagcgccag aggtgctgtt gggaaagttg
     3361 ctggcacaat ttcttatgga tataacgcaa ttaatattga tggcgtaaga tattttgcac
     3421 atagacttgc ctggctgtat gtatatggcg aatggcccaa acaagagatt gaccacattg
     3481 atagaaacag aagaaataac gcaatctcaa accttaggga tgtcagcaga gtcgtaaatg
     3541 cattaaatgt cggacctcga aatagcaatg ctggaatcaa gggggtaact ttctgtcaag
     3601 ccagaaatca atggcaagct cagataaatg tctcaggtaa aaacataacc cttgggagat
     3661 tcaacaccat tgatgaagcg gctatagctt ataaggcagc aaacatggtt gctgaccatc
     3721 tgttgagtag gtgatatctg acatttcatt gctccagacg aatgatatga tgcaaccatg
     3781 atgtgattgc ataccgaaat ggtactattg agtatttatc cagtaagtta cgatgccatt
     3841 ccacccaatt ggtgaggcat caaggatgta cagcaaatac gacgaggcgc agtttcactt
     3901 gagacttccg catgaactcc acgcgaaaat taagcagcgt gcgaagatga ataacaggtc
     3961 tctgaactca gagataattg cagcgattga agaatcactg gataaacaaa actctgcatc
     4021 agtttacatt gacgatgcag agcgtatggc agaacaacaa tccgatatgg ttaagaaaat
     4081 tgtctttgat acgctcaaag agctatataa aaaagacagc agctaaccat cagttacgga
     4141 ggatttatgc aaagagatat gctgaatatt gcgttctaca tatttggttt ttgcacgttc
     4201 ctggtgtttg cgaagctatt ctgacaacgc atcagacttg gcaccctgag tcagggcgtt
     4261 aatggccttt tgcgcctgct gcatggcttt ctcaaacgct gttgatccgc gtggggtatt
     4321 tgccattcgg agcattgcat ttctgaatgg ctcgctctca taggcgcgag taagaagtcc
     4381 gtagcttacc gctgcgccag ttgtcgccgg gttcattgcc gtcccatacc cgataatgaa
     4441 cgggatggtt tgctgccctg ttggtgttgt tactgccgct tttgctgcct gctgagtgga
     4501 ctgaaggtaa tttttcaatc ctttcagata agcagcgtcc tgccccttaa atgtgatgcc
     4561 agtctggttt tgcaggatgt taagctgccg aaggaactgg tcaggggatc cgccagattt
     4621 ctccatcgcc tttccaatga tgccattgcg catttgcacc ctgccaacac gaccaactga
     4681 gttatacagc gtcttaattt ccgatttgtt cttgctgaat agcatgttgt tgacaacttc
     4741 cggcgtcagg tcgcctttca tgagaacatt cttcagcctg gtattcttta gtttcgccgc
     4801 ttcgtcagcg tagacggcat tggcctgctg atatttacgg agagtatcgt tgccaagatt
     4861 ctgaccaatg gcaccattga tatcgtcggt catcgccttg taaacgcgct gaatggcagc
     4921 atcggaacgg tttggtaaca ctggtcgctc acccttcacg tccattctga actgactgcg
     4981 caggtcgctt aattgcttca aatccagatt taccggacct tcagggccag cattgcgaac
     5041 aagctcatca cgatatgact gaagttttga aattgtctcg ttatcagcca ccttaccaag
     5101 cttctgcagg ttagatattt ctgtatcaat ctgctgaatt gctcgcgcag gctgaatgtt
     5161 tactccagcc atagcattct gaacctgctc cagtcgatta ccggcggcac gacgaattcc
     5221 tgatgttttc gctttaaggc tgtcaataac aaccgctgga tcatactccc cgaatttatc
     5281 agcaaatctc tgcaccaact ggcttctcgc ttcctgttgc gttgctctca ttccgcttgt
     5341 gccagccaga gggatatttt ctgctgtagt ctgcgccatt tttccgacgc gggaagttgg
     5401 ttgtaacagg tctgtggtgt gcagaggaac tccttcacgc tctgcaaatc tgatagcctg
     5461 ctgagcttct ggcgcgatag caccacgaac gccacgataa gcagcaccta atccacgtcc
     5521 ggcagcgtta atagcaccgc cagcaagtac accaacgcct aaatcggtgg cgagtgcttc
     5581 cgcatcatct ttcgcactat ttgcagcaag tgatccaact gcgttttcag cgagaaggcg
     5641 agttgcaccc tgagcaattc gaccagcaag tgttggtgcc tgtgttgccg ctctctcaac
     5701 gccggcagga gtgaggtaag gcaatgcttc agcaaatacc ctgccctctg tcgtttgtgg
     5761 agtcagcgca ccttgctgaa ggccaaagtc ctgctctaat ccatgcgttg ttactcgtgg
     5821 cgctggttga tatgttccat cgccaatgcc gagtttaccg ccagcccatg cagccgcgct
     5881 tgttacagca tcggcaaccg atgcaggtat gtttgccacg ttcacgccag cctgcaccag
     5941 tccgcgacca gtctctttta ctgcttcgcc aagatcagac ataaatccac tttgctgtgg
     6001 ttgttgctgt gctactggtt gctgtgtctc cactggctgc acagatggca atggataggc
     6061 agcatagaaa gcttgcttag cctgctctgc attttctccg gcttgcgggg caacgacttc
     6121 attgaagtat tgctcctgag cctgcgcttt ttgttctggt gctaacgcct gatactgtgg
     6181 agatgcgata acatctttcc atgctttagc cattaatcac cccatagtga agaaaaatta
     6241 ctgccagcag gagattgttg ccctggcgta ttctgcaccg gctcctggta atcaaactgt
     6301 tttttaacag tgctcaactt gctttcaagc tgatttcgaa tctttccgat agagtcacga
     6361 aaagcctttt cactcatttt gggacttaga gcaccaaccg catcggataa ttttttaccc
     6421 tcagcatctg aaagagcccc catccccttc agggactgca ccatagggag gaaagtttga
     6481 gctttaaagg tgtcgagtct tgcttcaaag ttagccgcat cagatccagg aactgtcgga
     6541 aacgctgagc gaataccaac tgcttttgaa agtccagggc tttgctctat ctcgttgaga
     6601 gaatcaagcg cagtgctgaa cgtatcaaca gcgccctgag cggcggcctg tctgtcagca
     6661 cgagctattt cagccttttg ccgaacatct gcctgtttct gctttagctc ttcaagcttt
     6721 aactgattgc tttctctggc tatctgtctg tccagagcct tttcttgtaa ttctgctctt
     6781 tgtatttcgc gggaaagagc agcattctgt gcgctgatgt tctggccacg tatctggatg
     6841 tcctgacctc gtgctgttag tgcctcgcca gcctgattgc tgcggattgt ctctgccagc
     6901 ctacctcggt caatctcacg accagccatc ttgtcctgaa cattgaagta atcaatcgga
     6961 ccaagagcag ccatcccaag gtgatcaaca aactcaccaa atcctgaagg attctgctga
     7021 tacatctgag caacgttgtt aggatcaacg ccgacgcgag tcagttcct
//
LOCUS       DAJTYL010000164         7020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYL010000164 DAJTYL010000000
VERSION     DAJTYL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.164"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..711
                     /gene="hchA"
                     /locus_tag="OO572_003639"
     CDS             <1..711
                     /gene="hchA"
                     /locus_tag="OO572_003639"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6662488.1"
                     /translation="KHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFE
                     FEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAI
                     FVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSIC
                     AFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFA
                     ANALGKLAAQEMLAAYAS"
     gene            complement(819..2177)
                     /gene="hprS"
                     /locus_tag="OO572_003640"
     CDS             complement(819..2177)
                     /gene="hprS"
                     /locus_tag="OO572_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6662489.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2177..2848)
                     /gene="hprR"
                     /locus_tag="OO572_003641"
     CDS             complement(2177..2848)
                     /gene="hprR"
                     /locus_tag="OO572_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6662490.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2981..3394
                     /gene="hiuH"
                     /locus_tag="OO572_003642"
     CDS             2981..3394
                     /gene="hiuH"
                     /locus_tag="OO572_003642"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6662491.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3503..4507
                     /gene="msrP"
                     /locus_tag="OO572_003643"
                     /pseudo
     CDS             3503..4507
                     /gene="msrP"
                     /locus_tag="OO572_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4508..5143
                     /gene="msrQ"
                     /locus_tag="OO572_003644"
     CDS             4508..5143
                     /gene="msrQ"
                     /locus_tag="OO572_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6662492.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5401..6051
                     /gene="zinT"
                     /locus_tag="OO572_003645"
     CDS             5401..6051
                     /gene="zinT"
                     /locus_tag="OO572_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6662493.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1979 a         1590 c         1475 g         1976 t
ORIGIN      
        1 aaacataaaa ttctggtgat cgccgcggac gaacgttatt tgccgaccga taacggaaaa
       61 ctgttctcga ccggtaacca tccgattgaa acgttgctgc cgttgtatca tctccatgct
      121 gcaggtttcg aattcgaagt ggcgaccatt tccggtctga tgaccaagtt tgaatactgg
      181 gctatgccgc acaaagatga aaaagtaatg ccattctttg aacagcataa atcgttattc
      241 cgtaatccga agaaactcgc ggatgttgtc gccagcctca acgctgatag cgaatatgca
      301 gcaatctttg ttcctggtgg gcatggcgca cttattggtt taccagaaag ccaggacgtg
      361 gctgccgctt tacagtgggc aatcaaaaat gaccgttttg ttatctccct ttgccacggt
      421 ccggcggctt ttctggcact tcgccacagc gataacccac tgaatggtta ttctatttgc
      481 gcattcccag acgccgcaga caaacaaacg ccagatattg gctatatgcc gggtcatctc
      541 acctggtact tcggcgaaga gttgaagaaa atgggcatga acatcattaa tgacgacatc
      601 accgggcgag tacataagga ccgtaaacgt ctcaccggcg acagtccttt tgcagcgaat
      661 gcgttgggta aactggcggc gcaggaaatg ctggcagctt acgcgagtta atccctgtta
      721 cattgtgatg taagcgcata atcaatcgca gccacgttaa tactcacgac gtggctgcgt
      781 tttttcacgg ttaacttatg gcgtactgaa gcccgatgtt aatttctttg cggtaacgta
      841 attcggaaca cgttatgctt actgagatag tgatacgtag cacttcctcc atgtaattcg
      901 gcaatcgctt tgactaaaga aaggccaagt ccctgaccta cggaatgacg cgaattatct
      961 ccccgccaaa atctacggaa gagtttttca ggctcattaa ttttcgttcc aggactggcg
     1021 atatcaatat taagagagcc gttggcatca agaaaactgg ttatatgaat acgcgatttt
     1081 tctggtgaat atcgaatggc gttaacaata agattcgata acattcgttg cagtaaaatt
     1141 ttatccgcaa agatttgttg attgcacttg accttaaagc aaatctcttt ctcgtctgaa
     1201 agatattcaa gatagtccaa aagattttca acttccttat tgagagaaag cgaatccagt
     1261 ttcaccaaaa cattgttttt gtctgccctg gcaagaaaca gtatattctc tgttaaccgc
     1321 gaaatatttt ccagctcttc aatgtttccg gcaattgttt tttgatattc agcgatactt
     1381 ctgatttgac tgagcgtaac ctgattctga cccagtaatg cattaattgg cgttctaagt
     1441 tcatgagcga gatcgtcagc aaattgacta aggcgctcaa aatctttgac taaagcatga
     1501 tgcattttat tcaacgcctg cccaagaggt tttagttctc tcggtaatgc gttaacctca
     1561 accggcactc ggctatcgtt ataattcagc gcttccgtta cgccactcaa ctttttgatc
     1621 tctcgcaacc ccgtcctgat taacaacgga cttaatactg agcacagtat aatggcgaca
     1681 atgcaaatta taatgctatt aattttatac tgttcaagca tattatgtct ggctgaagcc
     1741 aatttagcca cagtaaccgt taatgatggc gaaacttcat caatgttaat tcgtaaagca
     1801 tctatctctg tatcattaat aatgcttctg taaatgcctg ctgcgctgat tgtctcacta
     1861 gcaggtatgt tatttaacat gtcatcactg acatttgtcc ggttaacaat tttattgatg
     1921 ccatcactat gaatgatcaa aatatcctga ctaacatcca tcatccggtt aaagtacaca
     1981 ggtaacgtat ctggatttac cccatcaatt aacaactgct tgatctgcgc tgtccggtta
     2041 atgagtgttg tatcatcgcg ccatttcaac tcacttgcca ggccattata gagagtccat
     2101 acaattccag cgccagcaac agactgcaga aatataaaaa gcaaggttaa acggacggtt
     2161 atagataatc ttttcattat ttttttaccg ctacgaatga atagcccatc ccccggattg
     2221 tggcgattag cttttcagga aaaggatcat caacttttgc gcggagcctg cgaatggcga
     2281 catccaccgt attggtatca ctatcaaagt tgattcccca aatttcactc gcaataaccg
     2341 ttctgggtat aatctcgcca gctctggagg ccagtagcca aagtaactga aactccttgc
     2401 gcgtcagtgt aatactgata ttgtccctgc tgacactttg actgacagag tccattttta
     2461 agccactgat ttctaatgtt gaattcagag cgtgatgttg ccttaattgt gcccgaaccc
     2521 ttgctagcaa ctcagaaaat gaaaaaggtt ttaccagata atcatttgcc ccactgtcca
     2581 accctctgac tctgtcatcg acagaatccc ttgcagtaag gcaaataaca ggggtttgct
     2641 ttgccgttct taacgtttgt aagatctgcc agccatccat acccggaagc ataatatcca
     2701 gaatgatcaa tgcataatca tccttcagcg caagataaag cccatctctg ccatcagaaa
     2761 cggcatcaat gacataaccc gcttcggaaa gcccctgcgt tacccattcc tgggtccttt
     2821 gattatcttc aataagtaga atcttcatga aaataatatg ccataaatca tatgttatag
     2881 aaacaacctg gttcattaca aaattgtaat gctgctgtaa ggttaccctg gacgtttttt
     2941 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
     3001 actctcggta gcaacggcag ccttttcatt accttctttg gtttatgccg cacaacaaaa
     3061 cattcttagc gtgcacattt tgaaccagca aacaggaaaa cccgctgccg acgtgacagt
     3121 cactcttgaa aagaaagcgg ataacggctg gttacaactt aataccgcca aaacagataa
     3181 ggatggacga attaaggcac tgtggcccga gcaaactgcg actactggcg attaccgtgt
     3241 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
     3301 tcccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tgttactcag
     3361 ccaatatggg tattcaacct atcgtggcag ttaatgcgta aatatgtaaa agctgatctc
     3421 tgctactgtt agggaggtga ctccctgaca gattaacagt aaacagctct tgttggttaa
     3481 cgacaaaaaa gtgtgatggc ttatgaaaaa gaagcaattt ttaaaagaat cagatgttac
     3541 ggccgagtcg gtattcttta tgaagcgtcg gcaggtgtta aaagcactgg gcatcagcgc
     3601 agctgcattt tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
     3661 cgatcgtccg cccgcccccg ccggaaaacc gctggagttc agcaagcctg ccgcctagca
     3721 aaataacctg ccactgacgc cagcagataa agtctccggt tataacaact tctatgaatt
     3781 cgggctggat aaagacgatc ccgcggctaa tgctggtagc ctgaaaaccg atccatggac
     3841 actgaaaatc agcggcgaag tggcaaaacc attgaccctc gatcatgatg atttaacccg
     3901 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtggaagcgt ggtcgatggt
     3961 ggtgccgtgg attggttttc cgctacacaa attgctggcg cttgccgaac ccaccagcaa
     4021 tgcgaagtat gtcgctttcg aaacgattta tgcgccggaa cagatgcctg gccagcagga
     4081 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     4141 aatgcatccg ctcacactga tgaccgtagg tgtttatggc aaggcgttac caccgcaaaa
     4201 tggcgcgccg gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     4261 cgtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     4321 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     4381 taccgaacga tttattggtt caggcggcat tctcgatgtt cagcgccagc caacactact
     4441 gtttaatggt tacgccgacc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     4501 tttctaaatg cgtctgacag caaaacaagt gatatggctg aaagtttgcc tgcatcttgc
     4561 cggattgttg ccgtttctct ggctggtctg ggcgatcaat cacggtggac tgggtgccga
     4621 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgcttgcggc
     4681 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     4741 cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct
     4801 ggagttgggc gtgaataatc tggcgttatt aggtaaggag ttaattaccc ggccttattt
     4861 aacgttagga attatcagct gggttatttt gcttgcttta gcgttcactt caacccagtc
     4921 gatgcagcga aaactcggca aacactggca acagttgcat aacttcgtct atcttgtcgc
     4981 gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc agccactcat
     5041 ctacgccggg ctggctgtac tgcttttagc cttacggtat aagaagttgc tttccctttt
     5101 caaccggtta cgcaagcagg cccacaacaa attatctcta taacttcata ttgccgacaa
     5161 acttcgcgtt atcccggccg gatgcggcgc gagcgcccta tccggcccac acagatcgca
     5221 tcaagttatg tttcgtttgt catcaacctt tcatcaccag aataatttat tttatttcaa
     5281 taagatagtt caatacacct gagaatacca tgctctcatt tcctaatgag ttgttgcatt
     5341 ttttcatatg ttacaatata acattacaca taatgtacat taactctgga ggaaattgtt
     5401 ttggcgattc gtcttcataa actggctgtt gctttaggtg tctttattgt tagcgctcct
     5461 gccttttcgc atggtcatca ttctcatggc aaacccttaa cagaggtcga acaaaaagct
     5521 gctaatggtg tttttgatga taccaatgta caaaaccgaa cgctcagtga ctgggatgga
     5581 gtctggcaat ccgtttaccc tttactgcaa agtggcaaac ttgatcccgt ctttcagaag
     5641 aaagcggatg cagataaaac caaaacattt gctgaaatta aagattatta tcgcaaaggt
     5701 tatccgacag atatcgaaat gattggcatt gaggacggca ttgtcgaatt ccatagaaat
     5761 aatgaaacaa catcctgtaa atatgattac gatggataca aaatactcac ctataaatca
     5821 ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc ctgaaagcaa agcccctaaa
     5881 tatatacaat ttagcgatca tataattgca ccacgaaaat catctcattt tcacattttt
     5941 atgggtaatg attcccagca atctttattg aatgaaatgg aaaactggcc aacgtattat
     6001 ccctatcagt tgagtagcga agaagtggtc gaggagatga tgtctcattg atttttttgc
     6061 gctgacttca gttatttaac agaacagtgc ctggtagtaa caacaataat ttaccaagac
     6121 aaataagtaa gacaaactca gacacgctca tacttgagtc gtgtcttttt tattatgtct
     6181 gtagaaaaaa actgagaaaa aggctaacaa taaatacaca actcagccat acagactggc
     6241 agcgcaaatg tttccaggag caagtcatct gtcatggtaa agctaaccag aaatataggt
     6301 taattttggt gtttaaacct tttttacttg ttagtatgat acaaaggctt tcaaaaaagc
     6361 tgggaaattc aacaactctt ggaaattatg gactttgtcc cgcggcttta atctgacggc
     6421 cgcgttcctt ttttggctca attattgacc ccttctctac gcaacttcag ttcccgccag
     6481 caactttgcg gcagtttcat aggatcaatg tctaaaagaa taatggtgac cgataagaaa
     6541 acgactgaat aactgtagat tttcgctcga aaccttcctg tcagctccat agcgaatcaa
     6601 gcgctgaatg tcacagtatc gaacagaaaa cagtgacgat ctaacccttc aagaatattc
     6661 tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg gagataagtc attgataaaa
     6721 tggcggagag agggggattt gaacccccgg tagagttgcc cctactccgg ttttcgagac
     6781 cgatgataca gagtcgaaaa atcaaagagt taaaaatttt tcttggaaaa aatgtcttaa
     6841 aatcgtacac gaaaaatcaa tgaattacat aacattaaag aagaatattc caagccttct
     6901 tgagtccacc acctgttttc aggtgtcatt cagacgtgat ttgccccaga ttgggaaagc
     6961 acgctttttt ctcagcttat tccgttttgc ctgtgtgttg aataattcca ctgcagttat
//
LOCUS       DAJTYL010000165         7014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYL010000165 DAJTYL010000000
VERSION     DAJTYL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.165"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..636
                     /locus_tag="OO572_003646"
                     /pseudo
     CDS             1..636
                     /locus_tag="OO572_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            641..1606
                     /gene="rbsC"
                     /locus_tag="OO572_003647"
     CDS             641..1606
                     /gene="rbsC"
                     /locus_tag="OO572_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6662494.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1631..2521
                     /gene="rbsB"
                     /locus_tag="OO572_003648"
                     /pseudo
     CDS             1631..2521
                     /gene="rbsB"
                     /locus_tag="OO572_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2647..3576
                     /gene="rbsK"
                     /locus_tag="OO572_003649"
     CDS             2647..3576
                     /gene="rbsK"
                     /locus_tag="OO572_003649"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6662495.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3580..4572
                     /gene="rbsR"
                     /locus_tag="OO572_003650"
     CDS             3580..4572
                     /gene="rbsR"
                     /locus_tag="OO572_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6662496.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4538..5965)
                     /gene="mdtD"
                     /locus_tag="OO572_003651"
     CDS             complement(4538..5965)
                     /gene="mdtD"
                     /locus_tag="OO572_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6662497.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(5988..6680)
                     /locus_tag="OO572_003652"
     CDS             complement(5988..6680)
                     /locus_tag="OO572_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6662498.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1780 a         1706 c         1852 g         1676 t
ORIGIN      
        1 tcgtaccgaa ctgatgaaag tgctctacgg cgcactgccg cgcaccagcg gttacgtcac
       61 cctggatggg catgaagtcg ttacccgttc accgcaggat ggcctggcaa acggcattgt
      121 gtatatctcc gaagaccgta aacgtgacgg tttagtgctg ggcatgtcag taaaagagaa
      181 catgtcgctg acagcgctgc gctacttcag ccgcgctggc ggcagtttga agcatgccga
      241 tgaacagcag gctgtgagtg atttcattcg tctgtttaat gtgaaaacgc cgtcgatgga
      301 acaggcaatt ggtctgcttt ccggtggcaa tcagcaaaaa gtggcgattg cccgcggtct
      361 gatgacaccc ccaaagtatt gatccttgat gagcctaccc gtggcgtaga tgtcggcgcg
      421 aaaaaagaga tctatcaact gattaaccag ttcaaagccg atggcttgag tatcattctg
      481 gtgtcatcgg agatgccaga agtattaggc atgagcgatc gcatcatcgt catgcatgaa
      541 gggcatctca gcggggaatt tactcgtgag caggccaccc aggaagtgtt aatggctgtc
      601 gctgtgggca agcttaatcg cgtgaatcag gagtaaaaaa atgataaccc agactgtctc
      661 tggtcgccgt tatttcacga aagcgtggct gatggagcag aaatcgctta tcgctctgct
      721 ggtgctgatc gcgattgtct cgacgttaag cccgaacttt ttcaccatca ataacttatt
      781 caatattctc cagcaaactt cggtgaacgc cattatggcg gtcgggatga cgctggtgat
      841 cctgacgtcg ggcatagact tgtcggtagg ttctctgttg gcgctaaccg gcgcagttgc
      901 tgcatctatc gtcggcattg aagtcaatgc gctggtggct gtcgctgctg ctctcacgtt
      961 aggtgccgca attggtgcgg taaccggggt gattgtagcg aaaggtcgcg tccaggcgtt
     1021 tatcgctacg ctggttatga tgcttttact gcgcggcgtg accatggttt ataccaacgg
     1081 tagcccagtg aataccggct ttactgagaa cgccgatctg tttggctggt ttggtattgg
     1141 tcgtccgctg ggcgtaccga cgccagtctg gatcatgggg attgtcttcc tcgcggcctg
     1201 gtacatgctg catcacacgc gtctggggcg ttacatctac gcgctgggcg gcaacgaagc
     1261 ggcaacgcgt ctttctggta tcaacgtcaa taaaatcaaa atcatcgtct attctctttg
     1321 tggtctgctg gcatcgctgg ccgggatcat tgaagtggcg cgtctctcct ctgcacaacc
     1381 cacggcgggg actggctatg agctggatgc tattgctgcg gtggttctgg gcggtacgag
     1441 tctggcgggc ggaaaaggtc gcattgttgg gacgttgatc ggcgcattaa ttcttggctt
     1501 ccttaataat ggattgaatt tgttaggtgt ttcctcctat taccagatga tcgtcaaagc
     1561 ggtggtgatt ttgctggcgg tgctggtaga caacaaaaag cagtaataac gactacagga
     1621 catcttgaat atgaacatga aaaaactggc taccctggtt tccgctgttg cgctaagcgc
     1681 caccgtcagt gcgaatgcga tggcaaaaga caccatcgcg ctggtggtct ccacgcttaa
     1741 caacccgttc tttgtatcgc tgaaagatgg cgcgcagaaa gaggcggata aacttggcta
     1801 taacctggtg gtgctggact cccagaacaa cccggcgaaa gagctggcga acgtgcagga
     1861 cttaaccgtt cgcggcacca aaattctgct gattaacccg accgactccg acgcagtggg
     1921 taatgctgtg aagatggcta actaggcgaa cattccggtt atcactcttg accgccaggc
     1981 aacgaaaggt gaagtggtga gccacattgc atctgataac gtactgggcg gcaaaatcgc
     2041 cggtgattac atcgcgaaga aagcgggtga aggtgccaaa gttatcgagc tgcaaggcat
     2101 tgccggtaca tccgcagccc gtgaacgtgg cgaaggcttc cagctggccg ttgctgctca
     2161 caagtttaat gttcttgcca gccagccagc agattttgat cgcaccaaag gtttgaacgt
     2221 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     2281 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     2341 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     2401 tatcgctcag ctacccgatc agattggcgc gaaaggcgtt gaaaccgcag ataaagtgct
     2461 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     2521 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     2581 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     2641 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     2701 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     2761 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     2821 gcgtttattg cctgtactgg cgatgacagc attggcgaga gcgttcgcca gcagctcgcc
     2881 actgataaca tcgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     2941 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     3001 gctctttccc catcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcgtta
     3061 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     3121 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     3181 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     3241 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa gggtatccgt
     3301 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     3361 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     3421 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     3481 catgctgccg ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     3541 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     3601 gcccgtttgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     3661 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     3721 tcagctctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     3781 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     3841 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     3901 ctggaaacgc tgatgcaaaa acgcgttgat ggcttactgt tactgtgtac cgaaacgcat
     3961 cagccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctaccgtgat gatggactgg
     4021 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     4081 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     4141 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     4201 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     4261 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     4321 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     4381 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     4441 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     4501 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     4561 ggttcggcat agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     4621 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     4681 attgttcgac agtcgttgtg ccttccatcc cttcataaat gcgaaggacg gccgcactta
     4741 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     4801 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aactgcgtcg
     4861 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     4921 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaatgtat
     4981 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     5041 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     5101 acatcaatgg cataaggaac ggtacacagc cggtccccag acgggttgca atattgccta
     5161 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     5221 tacgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     5281 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gaaaagagaa
     5341 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga
     5401 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattaatta
     5461 aaaatatcca gtgccaggtt gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa
     5521 ttggccccac cagacccggc atggcgacaa aattcaatac cggaagaagt tcattacgag
     5581 gataagcacg cagtaaggcc ggccgagcaa caggcatcat cattgcgtcg cctatccctt
     5641 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     5701 ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     5761 gccatccgct taccggaata agcatcgcca ccgtcagcgt ataactgatg atggctgatt
     5821 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     5881 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgaca
     5941 tactacgctt ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt
     6001 gagcgtagca gcgcctgaca agctttaaat gccgcgtcgc catcgctttg gataatcgca
     6061 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     6121 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     6181 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     6241 ttctctttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     6301 gtgccaaccg ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga
     6361 aagtgatcga tgacctgatg aaagttctct tctgttatcc accaagtaag caattcctga
     6421 tcaagaaaat tccagttcga ttgtggcatg acccgagtac caattcgcgg tcgcggtaaa
     6481 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     6541 aattgctcgc ccagctcaat ctcaccgggc aaaatggtgc cgggttcata ttcacctttt
     6601 aagatccgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
     6661 tgttgtgcgc ttaaaggcat tacttatctt cctttttctt tttattcctc cttagtatgc
     6721 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
     6781 cggggtgttt tgctgaaaaa atgcgcggtc agaaaattat tttaaatttc ctcttgtcag
     6841 gccgggataa ctccctataa tacgccacca ctgacacgga acaacggcaa atacgccgcc
     6901 gggtcagcgg ggttctcctg agaattccga cagaaaaagc aaaaataaat gattgactct
     6961 gtagcgggaa agcgtattat gcacaccccc ccgcgccgct gagaaaaagc gaag
//
LOCUS       DAJTYL010000166         6901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYL010000166 DAJTYL010000000
VERSION     DAJTYL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.166"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..567
                     /gene="espL1"
                     /locus_tag="OO572_003653"
                     /pseudo
     CDS             1..567
                     /gene="espL1"
                     /locus_tag="OO572_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            739..846
                     /locus_tag="OO572_003654"
     CDS             739..846
                     /locus_tag="OO572_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662499.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(899..1657)
                     /locus_tag="OO572_003655"
     CDS             complement(899..1657)
                     /locus_tag="OO572_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6662500.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1944..2873
                     /gene="pfkB"
                     /locus_tag="OO572_003656"
     CDS             1944..2873
                     /gene="pfkB"
                     /locus_tag="OO572_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6662501.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2974..3264
                     /gene="ghoS"
                     /locus_tag="OO572_003657"
     CDS             2974..3264
                     /gene="ghoS"
                     /locus_tag="OO572_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6662502.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3370..4230
                     /locus_tag="OO572_003658"
     CDS             3370..4230
                     /locus_tag="OO572_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6662503.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4271..4807)
                     /locus_tag="OO572_003659"
     CDS             complement(4271..4807)
                     /locus_tag="OO572_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6662504.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4954..5622
                     /gene="hxpB"
                     /locus_tag="OO572_003660"
     CDS             4954..5622
                     /gene="hxpB"
                     /locus_tag="OO572_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6662505.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5785..6375
                     /locus_tag="OO572_003661"
     CDS             5785..6375
                     /locus_tag="OO572_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6662506.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6508..>6901
                     /gene="tcyP"
                     /locus_tag="OO572_003662"
     CDS             6508..>6901
                     /gene="tcyP"
                     /locus_tag="OO572_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6662507.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFG"
BASE COUNT         1840 a         1647 c         1631 g         1783 t
ORIGIN      
        1 ctaacgaaac acctggactt tatatctcca tgttgtatgg attcgatgaa ataatcgata
       61 tctttctgaa tacattagcc actccaatag cactaagagc ttttaaacaa aaaactggtg
      121 atgagtattt tagccatgaa aatacatgat ggtgagccag gattatacgc cgcaatggaa
      181 aataatcacc ctttgtgtgt cacacggttc ctctctaaaa ttaatggcat cgcctttaaa
      241 tacaagttga gcaaagctaa catcatggat ttattaaaag gagctacagc acagggaacc
      301 cctgcattat acatcgccat gagcaagggt aatgaagacg tcgtgttatc ttatatatcg
      361 acgctgggtg cttttgcaaa aaaacattct tttagtcaac atcagttatt tacactattg
      421 gccgctaaaa atcatgacaa catgtcagct gttcatatag ccattcatca taagcattat
      481 aaaactgtag aaacatatta tgctgctata aatgtcatca gccaaagcct gagttttagt
      541 gctgatgaaa taaagacgta tttataacca gctatttacg atatatatcg atacataatt
      601 ttattttttt cataaaacgc agaaacagtg accatgtgca caacgccacc cgacggctgt
      661 aataacaaaa aatcccttga tgcctgtccc ttttgttaca ctccgttatc acgcacaaga
      721 gatatgcagg acactggtat gccgactaaa cgctttgata aaaaacactg gaagatggtg
      781 gtggtgctac tggcaatctg tggcgctatg ttgttgctac gttgggcagc aatgatttgg
      841 ggctgagcag tgacaaacga agacagccag accgcataac acggtccggc agataatttt
      901 acatgctgta gcccagcgat aaggttgtcc gacggtcggt atgttccggt gcagactctg
      961 gaggttcaga gttccatgtg acgttgtaag ccaccttcag tccaaaatgt tcattaatag
     1021 caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc cgcgccaaag accgaaacac
     1081 cctgcgtaaa tttagcgttg tcagtcaact gccatgcata agccccggag gcatagccca
     1141 gcggctgggt ttcactggca ttgtcggtat atttgtcgta acgcacacct ggaccgaatt
     1201 caaagcggaa actgtgtacc gggccattca aaaactgacg accataaccc gcggtcaaca
     1261 catcgcgctc tcgatagccg ttataacggt ctgtcagcca gcttgcctga ccaaataaat
     1321 agtcataatc agttaaatta aagcggctac gcccgcccgc cgcatatttt tctgaagaac
     1381 gctcatcatt agaagaagta ttactggcgt tcccccacag cgaccaggca gtggtttgtc
     1441 cataccaggt catggtggtg tcagccgtaa gtgaggagct tttcgtattg cctgattgtg
     1501 caagatatcc tgcgttcaga ttaccttcga aaggtttttt agcgctggca ggatcgtcca
     1561 tgacagtaaa aacggaatca tcggcagctg cattcagtga cgcaaacatg ccccccgcca
     1621 acataacgat ggcaggaact gtcttcaaaa gcttcattta tcaagagtcc gtacaacaaa
     1681 aaaagagacc atcgcggtcc cggaaacttt cttaaggatc aaagattagc gtccctggaa
     1741 aggtaacgaa ttataaaaag gcgcgaataa cttagcaatg tattcttatt tcattttttg
     1801 aataagcatg tggcgaaaac agatttttat ttatatatat ttatctgcaa aattttaaat
     1861 aaagctctaa taaatcatat tgttaatttc ttcactttcc gctgattcgg tgccagactg
     1921 aaatcagcct ataggaggaa atgatggtac gtatctatac gttgacactt gcgccctctc
     1981 tcgatagcgc aacaattacc ccgcaaattt atcccgaagg aaaactgcgc tgtaccgcac
     2041 cggtgttcga acccgggggc ggcggcatca acgtcgcccg cgccattgcc catcttggag
     2101 gcagtgccac agcgatcttc ccggcgggtg gcgcgaccgg cgaacacctg gtttcactgt
     2161 tggcggatga aaatgtcccc gtcgctactg tagaagccaa agactggacc cggcagaatt
     2221 tacacgtaca tgtggaagca agcggtgaac agtatcgttt tgttatgcca ggcgcggcat
     2281 taaatgaaga tgagtttcgc cagcttgaag agcaagttct ggaaattgaa tccggggcca
     2341 tcctggtcat aagcggaagc ctgccgccag gtgtgaagct ggaaaaatta acccaactga
     2401 tttccgctgc gcaaaaccaa gggatccgct gcatcatcga cagttctggc gaagcgttaa
     2461 gtgcagcact ggcgattggt aacatcgagt tggttaagcc taaccaaaaa gaactcagtg
     2521 cgctggtgaa tcgcgaactc acccagccag acgatgtccg caaagccgcg caggaaatcg
     2581 ttaatagcgg caaggccaaa cgggttgtcg tttcccttgg tccacaagga gcgctgggtg
     2641 tcgatagtga aaactgcatt caggtggtgc caccaccggt gaaaagccag agtaccgttg
     2701 gcgctggtga cagcatggtc ggcgcgatga cactgaaact ggcagaaaat gcctctcttg
     2761 aagagatggt ccgttttggc gtagctgcgg ggagtgcagc cacactcaat cagggaacac
     2821 gtctgtgctc ccatgacgat acgcaaaaaa tttacgctta cctttcccgc taacaaaaac
     2881 attcccccag cattggggga atcaccacca acctgtcggc aacgcgtttc tccgactatg
     2941 ctcaaaagtc atgtgataac aaaggggtga actatggcca gtggcgatct tgtccattat
     3001 gtcataaccg tcatgttgca tgaggataca ttgactgaaa ttaacgagtt gaataattac
     3061 ctgactcgcg acggtttttt gctcaccatg acggatgatg agggaaatat ccatgagctg
     3121 gggactaaca cttttggact tatcagtacc caaagtgaag aagaaattag agaactggtt
     3181 tcggggctta cccaaagtgc aaccggcaaa gatcctgaaa tcaccatcac gacctgggag
     3241 gaatggaata gcaacagaaa ataaatggtt tttgggcaat aatcagtctg tggtgtgcgt
     3301 tagctcgtgt ttttacaccg cattcttgcg ctaaccttat gatctggcag acaacatggg
     3361 agagacatca tgtggcaggc aatcagtcgt cttttgagcg agcagttagg tgaaggcgaa
     3421 atcgaactgc gtaatgaact gcctggcgga gaagtccatg ccgcatggca tttgcgctat
     3481 gcaggacatg actttttcgt caaatgtgat gaaagggaac tgcttcccgg ttttaccgcc
     3541 gaagccgacc aactggagtt actgtcgcgt agtaaaaccg tcaccgtgcc taaggtttgg
     3601 gcagtaggcg ctgaccgtga ctacagtttt ctggtgatgg attatctccc acctcgtccg
     3661 ctggatgcgc atagcgcatt tattcttggt cagcaaattg cgcgtttaca tcaatggagt
     3721 gaccaaccac aatttggcct cgattttgat aactcgctct ccacaactcc acagcccaac
     3781 acctggcaac gtcgctggtc aacgtttttt gctgaacaac ggattggctg gcagttggaa
     3841 ctggcagcag agaaagggat cgctttcggc aatatcgacg ccatcgtcga gcatattcag
     3901 cagcgtctgg cctcgcatca accgcagcct tctctgttgc acggcgattt atggtccggc
     3961 aactgtgcgc tgggtccgga tggcccgtac attttcgacc ctgcctgcga ctggggtgac
     4021 cgagagtgcg acctagcgat gttaccgctg catactgaac agccgccaca aatctatgac
     4081 ggctatcagt cagtatcccc gctacctgcc gatttccttg aacgtcaacc ggtttaccaa
     4141 ctctacacgc tgttaaatcg tgcaagatta tttggcggtc agcatttggt tattgctcag
     4201 cagtcattgg atagattatt agcagcatga tatgggttga ggataatggc cgctccgtgc
     4261 ggccttttga ttaaataaac ccaagcagag aaaagaacac ataacctgca gcaatacaaa
     4321 taaccggcag gatatatagt gagaaaaact gcaggaaaat agtatggtgc ggaacaacaa
     4381 tacgggactc aatttgttca cgcgttagcc cctcttcccc tttggctttt tccagaatga
     4441 gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg actcatccgg gcaccggaat
     4501 cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac ccagaacata acgttcacac
     4561 caccattaaa attaggggtc ggggagttat accagaacag attcaaaaaa ggcgtattcg
     4621 cctgcatcat atcgatcatg acatgagcaa agtcgagcat gaccgcatta atgccttcct
     4681 ggttttcctg ccgggtattc ataaatttca gcaaagaaat caatgtagag atcaatgcag
     4741 gtataaaaat cacccaaccc aaaatccttt tcaaaacagc aatgcgtcca gcttgttgat
     4801 acgtcatgag ttctccttga ttaagacgcg tcgtttcact tagtttacct gtagatatct
     4861 gttttcgccc attctttaaa ggcgatatga taggcgctta atcataagca cggcttaata
     4921 ccttacacat aatgctctaa aggagaggtt gtaatgtcaa ccccgcgtca gattcttgct
     4981 gcaatttttg atatggatgg attacttatc gactcagaac ctttatggga tcgagccgaa
     5041 ctggatgtga tggcaagcct gggggtagat atctcccgtc gtaacgagct gccggacacc
     5101 ttaggtttac gcatcgatat ggtggtcgat ctttggtacg cccggcaacc gtggaatggg
     5161 ccaagccgtc aggaagtagt agaacgggtt attgcccgtg ccatttcact ggttgaagag
     5221 acacgtccat tattaccagg cgtgcgcgaa gctgttgcgt tatgcaaaga acaaggttta
     5281 ttggtgggac tggcctccgc gtcaccacta catatgctgg aaaaagtgtt gaccatgttt
     5341 gacttacgcg acagtttcga tgccctcgcc tcggctgaaa aactgcctta cagcaagccg
     5401 catccgcaag tatatctcga ctgcgcagca aaactgggcg ttgacccact gacctgcgtg
     5461 gcgctggaag actcggtaaa tggcatgatc gcttctaaag cagcccgcat gcgttccatc
     5521 gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg tattagcaaa cgtcaaactc
     5581 tcatcgctga cagaactcac cgcaaaagac cttctcggtt aatgaccagg ggcagtgatc
     5641 acgctgcccc tggttcttta tctgaatttg cattcaactg acggattaat cgtcaattta
     5701 agagaaagag ttacaccgtc accacttccg tgcactgtat aaaaatccta tactgtacgt
     5761 atcgacagtt tagtgagttt tatcatgacg gcggaaggtc accttctctt ttctattgct
     5821 tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc tggcacaggg tgactggtgg
     5881 catattgtcc cttccgcaat cctgacgtgt ttgttaccgg acatcgatca cccaaagtcg
     5941 tttcttgggc agcgattaaa atggatatca aaaccgatcg cccgcgcttt tgggcatcgt
     6001 ggttttaccc acagtctgct ggcggtattt gcgctgctgg caacctttta ccttaaggtt
     6061 ccggaaggct ggttcattcc ggctgatgcg ctacaaggaa tggtgctggg ttatttgagc
     6121 cacatacttg ccgatatgct gacacccgcc ggtgttcccc tgctctggcc atgccgctgg
     6181 cgtttccgct tgcctatcct ggttccccaa aagggcaacc aactggaacg ttttatctgc
     6241 atggcattat ttgtctggtc ggtatggatg ccccattcat tacccgagaa cagcgctgtt
     6301 cgttggtcat cgcaaatgat caataccttg cagatccagt ttcatcggct tattaagcat
     6361 caggttgaat actaaaaagg caaaaatcac ctttctggaa taagcaattc catttgaata
     6421 taagagccag ctcacagttc tgttaatctt gcgccaacac tatgactgct acgcagtgat
     6481 agaaataata agatcaggag aacggggatg aactttccat taattgcgaa catcgtggtg
     6541 ttcgttgtac tgctgtttgc gctggctcag acccgccata aacagtggag tctggcgaaa
     6601 aaagtgctgg tgggtctggt gatgggtgtg gtttttggcc ttgccctgca taccatttat
     6661 ggttctgaca gccaggtact taaagattct gtacagtggt ttaacatcgt tggtaacggc
     6721 tatgttcaac tgctgcaaat gatcgttatg ccgttagtct tcgcctctat tctgagcgcg
     6781 gttgcccgtc tgcataacgc atctcagtta ggcaaaatca gttttctgac catcggtacg
     6841 cttttgttta ccacgctgat tgcggcgctg gtcggtgtgc tggtcaccaa cctgtttggt
     6901 t
//
LOCUS       DAJTYL010000167         6840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYL010000167 DAJTYL010000000
VERSION     DAJTYL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.167"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..215
                     /locus_tag="OO572_003663"
     CDS             <1..215
                     /locus_tag="OO572_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6662508.1"
                     /translation="VQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSDGEKQEVLRL
                     CRQRLMRKLRANKPASSDEPEQPSLF"
     gene            264..1160
                     /locus_tag="OO572_003664"
     CDS             264..1160
                     /locus_tag="OO572_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6662509.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            1241..1762
                     /gene="sodC"
                     /locus_tag="OO572_003665"
     CDS             1241..1762
                     /gene="sodC"
                     /locus_tag="OO572_003665"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6662510.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(1763..3775)
                     /locus_tag="OO572_003666"
                     /pseudo
     CDS             complement(1763..3775)
                     /locus_tag="OO572_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(3775..4632)
                     /locus_tag="OO572_003667"
     CDS             complement(3775..4632)
                     /locus_tag="OO572_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6662511.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(4635..4871)
                     /locus_tag="OO572_003668"
     CDS             complement(4635..4871)
                     /locus_tag="OO572_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6662512.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            5072..5506
                     /gene="slyA"
                     /locus_tag="OO572_003669"
     CDS             5072..5506
                     /gene="slyA"
                     /locus_tag="OO572_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6662513.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(5553..6020)
                     /gene="slyB"
                     /locus_tag="OO572_003670"
     CDS             complement(5553..6020)
                     /gene="slyB"
                     /locus_tag="OO572_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6662514.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            6294..>6840
                     /locus_tag="OO572_003671"
     CDS             6294..>6840
                     /locus_tag="OO572_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6662515.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQL"
BASE COUNT         1816 a         1707 c         1747 g         1570 t
ORIGIN      
        1 ccgtccagag cccgtgccgg ggaatttgcc agtctgacga acgcgggttt tgtcgcggct
       61 gttttcgtag tcgggatgag cgttttaact ggaataaaat gagcgatggc gaaaaacaag
      121 aggtgctgcg cctttgtcgg cagcgattaa tgcgcaaatt acgcgcaaat aagccagcgt
      181 catcagacga accggagcaa ccatcactct tttgaaccta atcttgcgta tactcctctt
      241 atatctgata agaggaagcg attatggttc agcgtattac tattgcgccg gaaggcccgg
      301 agttttcccg ttttgtgatg ggctactggc gattgatgga ctggaatatg tccgcccgcc
      361 agctggtcag ttttattgaa gagcatctgg atctcggcgt gaccaccgtg gaccatgctg
      421 atatttatgg tggctatcag tgcgaagcgg cgtttggcga ggcactgaaa ctggcacctc
      481 acctgcgtga acggatggaa atcgtcagta aatgcggtat cgcgacgacc gcgcgtgaag
      541 aaaacgtcat tggtcattac atcactgacc gcaatcacat cattaagagc gccgaacagt
      601 cgctaattaa tctcgcgacc gatcatctgg atctgctgtt aatccaccga ccagacccgt
      661 taatggatgc cgatgaagtg gcggacgcgt tcaaacatct gcatcagagc ggcaaagtgc
      721 gtcattttgg cgtatcgaac tttacgcctg cgcaatttgc cctgttgcaa tcacgtctgc
      781 cgtttaccct tgccactaat caggtggaaa tatccccggt gcatcagccg ttactgctgg
      841 atggcacgct cgaccaacta caacaactgc gtgttcgtcc gatggcgtgg tcctgccttg
      901 gtggtggtcg tctgtttaat gatgattatt tccagccgct gcgtgatgaa ctggctgtgg
      961 tggcggagga gttaaacgcg ggctcgattg aacaggtggt ttacgcctgg gtattacgtt
     1021 taccgtcgca gccgctgcca attatcggct caggtaaaat tgagcgcgta cgggcagctg
     1081 tcgaagcaga aacactgaaa atgacccgtc aacaatggtt tcgtatccgt aaagctgcac
     1141 tggggtacga cgtaccgtaa gcacttttag gaatagccgc cgttcaaaaa tgtgtcactg
     1201 gtttacactt attcaggaat gcacaatgaa cggaggtcct atgaaacgtt ttagtctggc
     1261 cattctggcg ctggttgttg cgaccggcgc acaagctgcc agtgaaaaag tcgagatgaa
     1321 cctcgtcacg tcgcaagggg ttgggcagtc aattggtagc gtcaccatta ctgaaaccga
     1381 taaaggtctg gagttttcac ccgatctgaa agcattaccc cccggtgaac atggcttcca
     1441 tattcatgcc aaaggaagct gccagccagc caccaaagat ggcaaagcca gcgccgcgga
     1501 atccgcaggc gggcatcttg atccacaaaa taccggtaaa catgaagggc cagaaggtac
     1561 cgggcattta ggcgatctgc ctgcactggt cgtcaataat gacggcaaag ctaccgatgc
     1621 cgtcatcgcg cctcgtctga aatcactgga tgaaatcaaa gacaaggcgc tgatggtcca
     1681 cgttggcggc gataatatgt ccgatcaacc taaaccgctg ggcggtggcg gtgaacgcta
     1741 tgcctgtggt gtaattaagt aattacgagg ccagcgtacc ttgcggtggt gcttgctcaa
     1801 gttgcgaaag cgagcagtac agccgccaga caattgccgc cagctcacgg gcggcaggtt
     1861 gatgatgacg ggcaaggctg tcgcaaatcc gctgtaattc ttcaagtgtg gccgccagtg
     1921 atttttgctg aacgccacgc tcactcatca ctccccgcaa cagtgaaata cagttatccc
     1981 gtactcgcga taacggatcg gaacgcgatt cccagtcgcg caattgccag acaacatgag
     2041 aacagttcag cagcactaca ccccagcgta ataaccaacg gcgagccagc gcatcctggc
     2101 tgttactcaa ctgactgacg tgatgataag tgagcgattc aaattcgctt tcactcagtg
     2161 ttggatggcg gcttagctga tcgacaaaat cccggcgcag cgcgcgaata tggcggcggc
     2221 ttttacgagc atccgatcct ggacgcagaa tggcgaacgc tagccaagcc agcgcaacgc
     2281 caacgatttt tgccagatta tagttaagaa aatcagcaaa atcatacacc gggggattag
     2341 tgacagcgat aaacgaaccc ataaaaacaa tcagttgccc ccacagtgcg gcaaatttgg
     2401 gcatctgcaa tttaagaagc tgcattgtcg ccagcagtgg aaagagaaac agtaaaaatt
     2461 gccacagatc gctaatctgg accatcaggc caaatttgac cacaaagctg aatagcgaaa
     2521 gtaacaccag cgtgcgcatc agcagtgaca acgacttaaa cggtgctgcg acggcggagt
     2581 agagtacgca gctaattgct gccagcgtta atgcattggc accggcatcc cattgcgatg
     2641 caatactcca tgcgccaatc atcatcaacg tacaaaatgt acgcagcccg ctccacatag
     2701 cttcggcgtt gtcggtatga cgagccagag cggatgtccg tgcgagtctg gcatgatcat
     2761 ctacaccgct ttgcagacga tgtaattcct gactactttg cagataaagg cggcaaaaat
     2821 aacgtagtcg ctgccagaag gcgacgtgcc ggtagtcggc gacgttggtc gggcgtagcg
     2881 gggcgataat acgtgcgacg gtgtaagcat ctgtttgcga actggcgagc gccgtcagca
     2941 attgttcgag aatttctcgc gtggcacctg gcggtgaggg ccagttgagc aacatacgtc
     3001 gcaggctgga gatgacactg gtcatacgta attgctggtg gagtagcgca ttaaggcgcg
     3061 cgttttgctg gcgaaaacga tagtggctcc agaaagcctg gatacgcagc aaattcatgg
     3121 tcagtatctg cccaatcacc ccctcatgtg ctgcacgaat ggcatcgttt gtttcaggct
     3181 gccagagtaa actggcatgt tccagtaatc gggcgtgcat gtttttcaat gcagttaaaa
     3241 gggcagtagc atcggaactg ctaggcagga tcatcatcat catgccgccg cacaaaatac
     3301 cgactatcac ctcgcaaacg cgcgcctgag cgatatccca cagctggctg gcctcagtaa
     3361 tattaaccat cggaaaggcg atgatggcag ccgtgtagcc cgccagttga aatgcatacg
     3421 cgacgttatt cgtgaagtgc gcacaggcct aggtacaaaa gccaagccac gccgacatgc
     3481 tcaatagaaa aaaccacggc tcattgagcg tatgtccggc aagaagcagt gccgcaatgg
     3541 ctcccagtaa actgccagcc atgcgtccga ggcttttgct gatgacaccg ccaacggtgg
     3601 gaaagctaac cactgcagcc gaggtcatcg cccaataggg ttcatccaga ttcaaataat
     3661 aggcaaccgt cagcgccaga cacatggcaa tggtattgcg taacgcataa cgccattgcg
     3721 ccagcgtggc cctgaaccag ggcaaattgc gcaaggacca tgacgatgcg ttcattattg
     3781 acctacggca atgctacagg tggtgcccga caccagcgtg acatcgcctg gaactttatc
     3841 cagcgcaaag cggacgggaa cgagttgggc gagacgaacc caggggacgt taggtttcac
     3901 atccggaata aggcttgagt cactttcaac gctctgatca taaatcgccc gaccgatact
     3961 ggaaacgtga ccctgtaacg ttttgttatc actatagagg gtaatttgtg ctggtgcacc
     4021 ttcgcggata tggcgcaact tggtttcttc aaaataacca atgacgtaaa acgagtggct
     4081 gtcgacaagg gcaaacagcg gtttcccggt gtcagcgtaa tcgccgatgc gggtagtgag
     4141 gttagtcacc catccactca ccggggcgcg aatttccgtt tgcgccagtt gccattgtgt
     4201 ctgtttcagc gtcgcctgtg cggcattaac actggcctgc atcgctttaa cattgaggtt
     4261 agcggtatcg agttcttcgg caaaaataaa attttgtgag agatgacgac gacgattcgc
     4321 ttcgttattg gcttttgcga ggtcagattg cgctttcgcc agctgagcct gtgcgttcag
     4381 ctcggcgatc tgaaagggcg ttttatcgat cgtgagcaga agttcccccg cgttaaccag
     4441 ttggttgtct tttatattca gctcaacaat acgtcccgac acctgtgggg tgatagaaac
     4501 ctgttctgcg cgtattttcc cgtcgcgtgt ccacggagat tgcatgtaat aattccacag
     4561 ccaccatccg gcaagaaccg caactatcgc tacaatgatt gttgagaaat acttaattgt
     4621 tttaattgac atagttacca cgcaatcagt attgccagag caaggcaaac gcaaatcgca
     4681 aacagcgata aatccattaa caagggatgc cagatatcgc cggcgtagat ccagccacga
     4741 agcaggcggt gtacgacaag ccagatgacg aatccaaacg cgaatgcttt gaaaaacgga
     4801 ggaaaataga cggacgcacc gaacaccaga tcttgtaagg gcaatcctgt tgcattgagc
     4861 ataaacttca caaagtagat tcctttacga ccgaatatat tgcgttagat taataaatat
     4921 tctttaagtg cgaaaaattt acgcgcaatt tctgaaaaat atgtctgacg gtaaccaaat
     4981 gcagcaatac atttgtttta gcaatacaat tgctgcacac tattctaaaa gccgcataat
     5041 atcttagcaa gctaattata aggagatgaa attggaatcg ccactaggtt ctgatctggc
     5101 acggttggtg cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc tggagttaac
     5161 acaaacccat tgggttacgt tacacaatat ccatcagtta cctccagacc agtcgcaaat
     5221 tcaactggca aaagcgattg gcatcgagca gccatcactg gtccgtactc tggaccaact
     5281 ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg ctaaacgtat
     5341 taaactgacg gaaaaggcag agccactgat cagcgaaatg gaagctgtta ttaacaaaac
     5401 ccgcgcggaa atattacatg gcatctccgc agaggaactg gagcaactga ttacgctcat
     5461 cgcaaaactt gagcataata tcattgagtt acaggccaaa gggtgaaatg aagggggcgt
     5521 gtggtcagta acctgaccac acgcaaactt atttagcgcg gagaaacggt cacctgactg
     5581 ccattgctgg ccagtacgac acgttggccc ggagagaaac gagtgttgcc ttgtttctgt
     5641 accaccatga tggtattacc atcgtcttta cgaatttcca gctcgacacc ctgcgttttg
     5701 ttcattgcac tctgtacgcc ctgaccagct acgccacctg caacagcgcc tgctgcagta
     5761 gccagagaac gcccggttcc gccaccaaca gtattcccca ggaaaccacc aagaacagca
     5821 ccgccaattg caccgataac gttggaatca tcaccgccct gaatctgtac cggacgtacg
     5881 ttaacgatgg tgccatagct gacattctgt acttgtttcg cttcagaagc ggtataaaca
     5941 tcccctgaca gggtgtcgtt attaacacaa ccgacaagag acagacctac cattgaaaca
     6001 accaatacgc gtttaatcat tgaaaaatct cctgttcacc atgaaacgcc acgcaagcat
     6061 ccctcatggt caaagtatat ggcatatgtg aatccatggt tacatgttct accaaatatg
     6121 agcaaatcat aatgaaagta ggatcaacca attataaaca aaacattcat gatgattatt
     6181 gatttgaaga taatgtttta ctaccaacgg taaaaatcgg tcattccgca tggtattcac
     6241 gaaaagttta tgtttgagtg gtgcgcaaac gtcagattga aaaaggatga actatgaaat
     6301 cgggccgctt tattggcgtt atgtcaggca ccagccttga tggtgttgat gttgtgttgg
     6361 cgacaattga tgaacaccgg gtcgcacagc tggcaagctt gagctggccg atcccggtgt
     6421 ctctgaaaca ggctgtactg gatatttgcc agggccagca gcttacactt tcgcagtttg
     6481 gacagcttga tactcaactc gggcgacttt ttgctgatgc ggtcaatgcc ttgcttaagg
     6541 aacaaaacct gcaggcgaga gatatagttg cgatcggctg tcacggtcaa accgtctggc
     6601 atgaaccgac gggcgtggca ccacacactt tacagattgg cgataacaat caaattgtgg
     6661 cacgcaccgg aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac
     6721 aaggcgcacc gctggtacct gcgttccatc atgccttgct ggctcaccca accgagcgac
     6781 gaatggtgct caatattggc ggcatcgcca atctgtcact gctcattcct gggcagctgg
//
LOCUS       DAJTYL010000168         6810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYL010000168 DAJTYL010000000
VERSION     DAJTYL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.168"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(68..970)
                     /locus_tag="OO572_003672"
     CDS             complement(68..970)
                     /locus_tag="OO572_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6662516.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1049..2002)
                     /gene="lsrR"
                     /locus_tag="OO572_003673"
     CDS             complement(1049..2002)
                     /gene="lsrR"
                     /locus_tag="OO572_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6662517.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2251..3786
                     /gene="lsrA"
                     /locus_tag="OO572_003674"
     CDS             2251..3786
                     /gene="lsrA"
                     /locus_tag="OO572_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6662518.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3780..4808
                     /gene="lsrC"
                     /locus_tag="OO572_003675"
     CDS             3780..4808
                     /gene="lsrC"
                     /locus_tag="OO572_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6662519.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4808..5800
                     /gene="lsrD"
                     /locus_tag="OO572_003676"
     CDS             4808..5800
                     /gene="lsrD"
                     /locus_tag="OO572_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6662520.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5812..6810
                     /gene="lsrB"
                     /locus_tag="OO572_003677"
                     /pseudo
     CDS             5812..6810
                     /gene="lsrB"
                     /locus_tag="OO572_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1558 a         1738 c         1796 g         1718 t
ORIGIN      
        1 tctgactaaa tagacatgtc cgcgctacag tctcaccaca gcgatcactg ctttcaaagg
       61 atgggactca tgacggcata cggaacggag taatcggcaa ctgatgccgg gcctcgctga
      121 gcgtgcactg cttattatcc gtgaacgtgc cgccaagaac cgcggtttgt gccggacgca
      181 caacgcctaa cccaaggcaa ccaagctgca cgtcgcctcc tccaacgacc accagagtgc
      241 ccgccttcag accgcagagt tccgccgctt gtgaacttac cacgcccagc aatgtgccgg
      301 tttctttgac aggagaaaga atatcggcac gcaggccagc catatccagc aatgcaggtt
      361 tccaggcacg ggtggttaga tcaagaagtc ccgtggtgcc agcgttagag ggatccaccg
      421 ccagttcgcc gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct
      481 gacggtaaat atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcacttaaag
      541 ccagtgtttg tccggtcgct cgataaactt cgttttcaaa ggtattgttg tgcagttctt
      601 taagttcgct aacttcgcgt gccgctctgg catccacatt ggcgcaggcc cagatcggtg
      661 ttccttcatt gttatataaa acaatgcctt cacgcataga acatgccgaa acggcagcga
      721 tatactccgg gtctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt
      781 gccagttttt gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc
      841 gccactccgc ctgtcccact gctatttgat tgccttccag gtcgaatatc acagcccgaa
      901 tacttccggt gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga
      961 gtcgagccat tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca
     1021 tcattcccgg taataaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct
     1081 gatcggtaac cagtgcgttg atataaccgc ctttcattgc agcggcaatt gcttcagctt
     1141 tattttctcc ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta
     1201 agccaatcag ttcgttatgt attttgatat ccgtgacaac gtcacctttt gcatcaaaaa
     1261 agtagcctaa aatgtcgcca accgcccctt ttcggccaat cattaactgt tcgccctggc
     1321 tgatataacc ggagcgaatg attgtcgcgt cgtcttgttg actcacggct ccaatgccga
     1381 caatcgccac atccgctgct tgcgcggcta gcagaacatc tttgacgcaa ttttcatttt
     1441 ttagcgtacg ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac
     1501 tgcacgccgc gttaagctgc ccgatacccg tcatataaga accgacgcca ccggagagcg
     1561 tgaccaggcg aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatgg
     1621 ttgcctcgcc aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat
     1681 gcgccgcgcc tatccctagt cgcccgccga catcagcatc cgcaagccca gggatcaccc
     1741 ggacatgttg cagcgaaaac tgacgacgta attgagtttc atattccaga cagccttcaa
     1801 agcgagaatt aatctgtacg cgaataatgc cggactgatg ccctttctcc agcaagcgcg
     1861 acactttcaa acgggtcagg ccgagacgat cgctgatctc gctctgggtc agcccgtcgt
     1921 gatagtaaaa ccacgcgatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa
     1981 ttgccgaatc gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat
     2041 gcaattgttc agttcttgcg catttatatc tgtgatggca accacagttt gactctacga
     2101 gcatgaacaa acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa
     2161 atatgtgcaa tgtccaccta aggttatgaa caaattaaaa gtagaaatac atttgttcaa
     2221 aactcacctg caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg
     2281 ctactttgcg cccgctcggt ttataaacag tattcagggg tcaatgtcct gaaaggcatc
     2341 gattttacgt tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa
     2401 tcgacgttaa tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt
     2461 gggggcaaca actacgccag attaacgcca gttcatgctc atcagctggg catttatctc
     2521 gttccccagg aaccgctgct tttcccaagc ctgtcgataa aagaaaacat cctgtttggg
     2581 ctggcaaaaa aacagctctc catgcagaaa atgaagaact tgctggcggc gctgggctgc
     2641 cagtttgatc tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa
     2701 atcctccgcg ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc taccgcctcg
     2761 cttacccctg cggaaaccga acgcttgttt agtcgcttgc aagagctgct tgctactggc
     2821 gtgggtattg tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt
     2881 agcgtgatgc gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac
     2941 gacattattc aggccatcac cccagcggta cgggaaaaat cgctctctgc cagccaaaaa
     3001 ttatggctgg agttacctgg taaccgccca caacatgccg tcggaacgcc ggtgctgaca
     3061 ctggaaaatc tgactggcga aggcttcagg aatgtcagcc tgacgctcaa tgccggagaa
     3121 attctgggcc tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat
     3181 ggtctgcgta ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caataaatta
     3241 tccactggag aacgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc
     3301 ggactgaatc tcgatgcttc gctggcctgg aacgtctgcg cccttactca taaccttcgt
     3361 ggattctggg cgaaaaccgc gaaagataat gccaccctgg aacgttatcg tcgggcgctg
     3421 aatattaaat ttaaccaacc ggaacaagct gcacggacat tatccggtag caaccagcaa
     3481 aaaatcctca ttgccaaatg cctggaagct tcgccgcaag cattgattgt cgatgagccg
     3541 acgcgcggcg tggatgtctc ggcccgtaat gatatctacc agctgttgcg cagcatcgcc
     3601 gcacagaatg tggctgtgct gtttatctcc tctgatatgg aagagatcga actgatggca
     3661 gatcgtgtgt atgtgatgca tcagggcgaa attacccact ctgcactgac cgggcgcgat
     3721 attaatgtcg agactattat gcgcgttgcc ttcggcgata gtcagcgtca ggaggcatca
     3781 tgctgaagtt tattcagaac aaccgtgaaa tcacggcatt gctggcggtg gtgctgctgt
     3841 ttgtattacc cggttttctc gaccgccagt atttaagtgt gcaaacgctg accatggttt
     3901 atagcagcgc gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc
     3961 gcaatattga tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt
     4021 tactgaacgc aggatattca ctacctgttg cctgtgtcgc gactttactg cttggtttgc
     4081 tcgcgggatt tttcaacggt gccctggtcg cgtggctaaa gatccctgcc attgttgcca
     4141 cccttggcac gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga
     4201 ttgaagggtt acccgccgaa ctgaaacagc tctccgcccc gctgctgctt ggcatttcag
     4261 caattggttg gttgacgata attctggtgg catttatggc ctggctgctg gcgaagacgg
     4321 cgtttggacg cagtttttat gccacgggcg ataatttaca gggcgctcgt caactgggcg
     4381 ttcgtacgga agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg
     4441 cgggaattgt gtttgcttcg cagattggtt ttatcctcaa ccagaccggt accgggctgg
     4501 agatgaaagc aattgcagcc tgcgtgctgg gcggcattag tttgctcggt ggttccggtg
     4561 cgatcatcgg tgcggtactc ggcgcatggt tcctgacgca gatcgatagc gtactggtgc
     4621 tgttgcgcat tccggcatgg tggaatgatt ttatcgcggg tctggttctg ctggcggtgc
     4681 tggtgtttga tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg caaaaatatg
     4741 cccgctttat gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg
     4801 ccgcataatg cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga
     4861 gattgtcgca tttggtgcaa ttaacccgcg aatgttagat ctcaatatgt tgctgttcag
     4921 caccagtgac tttatctgca ttggcattgt cgctctaccg ctgacgatgg tgattgtcag
     4981 tggcgggatc gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
     5041 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
     5101 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
     5161 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
     5221 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
     5281 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
     5341 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     5401 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     5461 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     5521 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     5581 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     5641 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     5701 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     5761 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     5821 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     5881 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     5941 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     6001 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     6061 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     6121 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     6181 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     6241 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     6301 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     6361 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     6421 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     6481 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     6541 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     6601 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgtattatt
     6661 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     6721 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     6781 gttaccggag cgcgtgatat tcaacaaaga
//
LOCUS       DAJTYL010000169         6710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYL010000169 DAJTYL010000000
VERSION     DAJTYL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.169"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(436..681)
                     /locus_tag="OO572_003678"
     CDS             complement(436..681)
                     /locus_tag="OO572_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6662521.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(815..2200)
                     /gene="puuP"
                     /locus_tag="OO572_003679"
     CDS             complement(815..2200)
                     /gene="puuP"
                     /locus_tag="OO572_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6662522.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(2190..2354)
                     /locus_tag="OO572_003680"
     CDS             complement(2190..2354)
                     /locus_tag="OO572_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662523.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(2503..3921)
                     /gene="puuA"
                     /locus_tag="OO572_003681"
     CDS             complement(2503..3921)
                     /gene="puuA"
                     /locus_tag="OO572_003681"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6662524.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            4133..4897
                     /gene="puuD"
                     /locus_tag="OO572_003682"
     CDS             4133..4897
                     /gene="puuD"
                     /locus_tag="OO572_003682"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6662525.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            4924..5481
                     /gene="puuR"
                     /locus_tag="OO572_003683"
     CDS             4924..5481
                     /gene="puuR"
                     /locus_tag="OO572_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6662526.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            5631..>6710
                     /locus_tag="OO572_003684"
     CDS             5631..>6710
                     /locus_tag="OO572_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6662527.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREG"
BASE COUNT         1702 a         1762 c         1750 g         1496 t
ORIGIN      
        1 gctgacgcaa tagacaaaac cgctgtcgcg gatatcagca tgcggggggg attcaggcgc
       61 ggcgattgcc tgaccactca caagtccagc aatcaccaaa agagacgata atacctggcg
      121 cataatatta agggatttta tgtaaagagg ctatcttact aatatttaat gacatttgcc
      181 attaccgttt gtgttcaggg gtcgtaatga gggccgtgtg gttggtcgtt gaacaggtgg
      241 cgtcgccatc tgttcaacat tcgtacccgt gatttctcta taactataac tcacagaaca
      301 acttagcgag gagcagagcg gtacgatcgt gagaaaccca ttaaagcacg ctgcagtgcg
      361 tgtcattgtt agccggatgc ggcgtgaacg ctttatccgg acaacgatac tgaccgatcg
      421 tctgcaatta aataattact cattacccca ttgattcaaa aactctgcga tctcatcaat
      481 gcgtacggga ttaatccccg cttcagcagc catttcatgt tgggcttctt cgctgatctc
      541 ttcattgttc atcaaacggg tgagcagtaa ctggaagtag tgggccagcg gtgtgcgttc
      601 agcctctgcg acaggctctg cgcattcggt cgcgtactca tcggcaatat caaaatattt
      661 tagcgggata tcgtggttca ttatttgccc ctggggttaa tgcgctaagc ataagggcgg
      721 atgatagcac tcgtgatccg ggtcatacat ctttttatag ttaatggcgc ggcgcgttac
      781 cctcaggcag gataatgcgc cgcgcatccg gctattacgt ttcgctcacc ggcgttctgt
      841 caccttcgta cagcggcact ttgcgatagc ggcggatcag ataccacaaa tatgcgccgc
      901 ctaacgaagc ccataccaga ccgagtgtca gtgacgttga ctctaggtta acccacagca
      961 cacccaccgt cagcgcgcca accagcggca tcagcaaata gtggaagtga tccttccagc
     1021 ttttattcat tcctttacgc cgccagaaat gattaaacac cgacagattc acgaaggtaa
     1081 acgccaccag agcaccgaag ttaatcagtg ctgtcgcggt gactaaatca aagaacagcg
     1141 ccgacagcgc gacaatcccg accataatga cgttgagtgc cggagtccgc cattttgggt
     1201 gcacatagcc aaacacgcgc tccggaaaca cattgtcacg ccccatcaca tacagcagac
     1261 gcgacacgct ggcatgtgaa gccaggccag acgctaatgt gttcacaaac gtggtgcaga
     1321 ggaaaatcga ctgaaagagc ttgccgccga cgtacagcgc aatttcaggc agtgcggcgt
     1381 ccgggtcttt aaagcggctg atatcgggaa agaacagctg cataaagaac gacgccgcga
     1441 taaagataac gccgccgtat accgccgtca ggaagatggc tttcgggatc acgcgtgcgg
     1501 cgtccggcgt ctcttccgaa agcgtggtca ccgcatcgaa accgaggaac gagaaacaga
     1561 caatcgtcgc accggtaata atcgggatca ggtgcgcgtt ctcgctgata aacggctgaa
     1621 gtgaccagac ggtgccaacg ccttctcctt tatgcagtcc ctgaaccacc aggaagataa
     1681 acaccaccat gatggagatt tgcaccagta caaacagggt attgaagtta gcgaccaggt
     1741 tgacgctctt cagattcgcg gcggttaaaa tggcgacgaa ggttaccacc cacacccacg
     1801 gcggcacttc cgggaagagg gcggagagat agattttcgc caacaaaacg ttgatcattg
     1861 gcaaaaagag ataatccagc agtgatgacc agccgaccat aaatccgacg tgcgggttaa
     1921 tcgacttttg cgcgtaggta taggccgaac cggcctccgg aaactggcga accagtttgc
     1981 cgtagctgat agcggtaaac agcacgcccg ccagcgccag caaataggac gccggaacgt
     2041 gaccgtcgct aatgccggag acaataccaa aagtatcaaa tacggtcatc ggcgtgagat
     2101 aggccagacc catcattacc acctgccaca atttcagtga ttttcgcaga cgggttttgc
     2161 cgggttgcgc agcaatattc agtggtgaat taatagccat aattgctgcc tcccctgcgt
     2221 aataaggttt gcggtcgtgt cgacctgaaa tcaaacctgt ttagcaaggg aagcaaaggg
     2281 ggcggagtcc agtcagtagc aggaggggcg catacgcagc ctccctcgcc atgcggggat
     2341 ttaatcatcg gcatcatctc atttcctcgt cacagtctta atcgttaacg gaagcccgtt
     2401 gccgcctgcg ctccataacg cgacatccgg gaaatgtctg gattaccagc aaatgtcagg
     2461 cctggctccg ctcaggccga tgaaacaacc ccgcaagggg aattacgcgt ttttcaacat
     2521 ccactcaatt tctgtttctg tgatgaggcg ctcaaactgc aacagctcat catttttaca
     2581 ggcgtgatac acatggcaaa accgttcgcc taaatagcgg cgcaggtgat cattctcgat
     2641 aaactcaccc agggcatcgc tctggcgaat cgggaagggt aagccttcct gttccagccc
     2701 gttgccttcg acttcttcct gcaacggcag ctcgttatca aggccatgca aaataccggc
     2761 aaaaatcgct gccatcacca gatatgggtt ggcatcggca cccgccacgt gatattccac
     2821 gcggtgatta tgacggtcac cgcacggaat acgcagggcg acggtgcggt tgttatggcc
     2881 ccacgacgcc tgcgtcggca catacattcc cggctggaag cggcgatacg agttcacgtt
     2941 tggtgccagc aacgccatcg acgacggcat caggtcaatc atcccggcga gcatcttttt
     3001 cagcagcggc gaatcttcgc cttctgtgtc agaaagcaca ttctcgccac gattattttg
     3061 catactgata tggatatgca tcccgctgcc cgcgtgctct tcatacggct tcgccataaa
     3121 agtggcgtgc atcttatgct tttctgccat cagacgcact aggcgtttta gtgccagcgc
     3181 atcatcgcag gcttccagca cgttatcagt atggtaaagg ttgatttcaa actgacccgg
     3241 cgaggcctca gcgaccgcgc catctgccgg aatcagctgc aactgcgcca gttcatcaat
     3301 atcattgagc acgtcggcga agtggttgag gttatcaacg gagtaaacct ggctttgtgt
     3361 attgcggtca tcggtgccag gcgcgcaggg cggttgcaga tccccttcag cgtcgcgctg
     3421 gcgatccagt aaatagaact ccagctctac cgctacaacc gggaacaatc cgcgctggcg
     3481 cagctgctgc cacaggcggt taagaacgtt ccgcggctca acgtcaaagg gagcgccatc
     3541 ttcatcgacc atggtcagga gcatctgacc gataaactct ggatcggcgg cagaaggagt
     3601 taaggaaccg agaacaggaa cacaggtacg atccggctcg cccatttcct gacccagacc
     3661 tgcttcttct actacgttgc ccagaatatc cattgcaaat accgaggccg ggaaataaca
     3721 ccctttctcg agcttcttca ggcttgaaac agggatacgt tttccacgga agcaaccatt
     3781 caaatcagta agtaaaacat caacatattg cgtattcggg tagcgctcca ggtagcgttt
     3841 cacttcctgc gtaaaggcgc taccccgcct ctcttctgac tgctgaacaa agttctctac
     3901 ttcaacgata ttggtttcca tgattcttcg cctttggttt gttttccgct cgttatcaaa
     3961 gcgtaaaata taatgaccat cattcgaatc tgtatgcaaa ccaaatgttt gtcaaatgtt
     4021 aaattgagtt tgcaaaaatg aaaatccact gctagattga aaaaatattg aacataaagg
     4081 tcatttaaag cgcagtaatg gcgataattt agtccatttt gtgagattga gcatggaaaa
     4141 tataatgaac aatccggtta tcggtgtcgt aatgtgcagg aacaggctta agggtcatgc
     4201 gacccagact ctgcaagaaa agtacctgaa tgccatcatc cacgcaggcg gcttgccaat
     4261 tgcgctacca catgcgctgg cggaaccgtc gttacttgaa caacttttgc cgaaactcga
     4321 tggcatttat cttcctagta gtcccagcaa tgtgcagccg cacctatatg gtgaaaacgg
     4381 cgatgagcct gacgccgatc ccgggcgtga tcttctgagc atggcgataa ttaatgccgc
     4441 actcgaaagg cgcatcccca ttttcgccat ctgccggggt ttacaagaac tggtggtggc
     4501 aaccggtggt tcgttgcatc gcaagctgtg cgaacagcct gaattgctgg aacatcggga
     4561 agatcccgaa ctgccggtgg aacagcaata tgcaccgtcg catgaagttc aggttgaaga
     4621 ggggggatta ctgtctgcgt tgttacctga atgtagcaac ttttgggtaa actctctaca
     4681 tggacaaggg gcgaaggtcg ttagcccacg gttgcgtgtt gaagctcgct cgccggatgg
     4741 cttagttgag gcggttagcg tcatcaatca tccttttgcg ctgggcgtac agtggcaccc
     4801 ggaatggaac agtagcgagt acgcgctttc gcgtatattg ttcgagggct ttatcaccgc
     4861 ttggcagcac catatcgctg aaaaacagcg actctgacca ctacagttta aggaaatgca
     4921 aatatgagtg atgagggact ggcgccagga aaacgcttgt cggaaatccg ccagcagcag
     4981 gggctttcac aacgtcgtgc cgccgaactc tccgggctga ctcacagtgc tatcagtacg
     5041 atagaacaag ataaagtcag ccctgccatc agtacgctgc aaaagctgct gaaggtgtat
     5101 ggtctgtcac tctcggaatt cttttccgag ccggaaaaac ctgatgagcc gcaggtcgtc
     5161 attaatcagg acgacttaat tgagatgggc agtcagggtg tgtcaatgaa gctggttcat
     5221 aacggtaacc cgaatcgcac gctggcgatg atctttgaaa cgtaccagcc gggcacaacc
     5281 actggggaaa gaattaagca tcagggtgag gaaataggca cagtactgga aggtgaaatt
     5341 gttctgacga ttaatggtca ggattaccac ctcgtcgcgg ggcaaagtta tgccattaat
     5401 accggcatcc cgcacagttt cagtaatacg tcggcaggta tttgccgaat tatcagcgcc
     5461 catacgccca ccacgtttta atctttttgt tctgtaagcc gggtaagcgc aatgcgcccg
     5521 gcaatccata aagaatgtcg ccagaaataa acgggcatgc agcccgggga tctctgcgcc
     5581 ctgacgttca caaactgcat atatctgata gacgtgaaac aggagtcata atgaattttc
     5641 atcatctggc ttactggcag gataaagcgt taagtctcgc cattgaaaac cgcttattta
     5701 ttaacggtga atatactgct gcggcggaaa atgaaacctt tgaaaccgtt gatccggtca
     5761 cccaggcacc gctggcgaaa attgcccgcg gcaagagcgt cgatatcgac cgtgcggtga
     5821 gcgcagcacg cggcgtattt gaacgcggcg actggtcact ctcttctccg gctaaacgta
     5881 aagcggtact gaataaactc gccgatttaa tggaagccca cgccgaagag ctggcactgc
     5941 tggaaactct cgacaccggc aaaccgattc gtcacagtct gcgtgatgat attcccggcg
     6001 cggcgcgcgc cattcgctgg tacgccgaag cgatcgacaa agtgtatggc gaagtggcga
     6061 ccaccagtag ccatgagctg gcgatgatcg tgcgtgaacc ggtcggcgtg attgccgcca
     6121 tcgtgccgtg gaacttcccg ctgttgctga cttgctggaa actcggcccg gcgctggcgg
     6181 cgggaaacag cgtgattcta aaaccgtctg aaaaatcacc gctcagtgcg attcgtctcg
     6241 cggggctggc gaaagaagca ggcttgccgg atggtgtgtt gaacgtggtg acgggttttg
     6301 gtcatgaagc ggggcaggcg ctttcgcgtc ataacgatat cgatgccatc gcctttaccg
     6361 gctccacgcg tacgggtaaa caactgctga aagacgcggg tgatagcaac atgaaacgtg
     6421 tctggctgga agcgggcggc aaaagcgcca acatcgtttt cgccgattgt ccggatctgc
     6481 aaaaagcggc aagcgccacc gccgcaggca tcttctacaa ccagggccag gtgcgcatcg
     6541 ccggaacgcg tttgttactg gaagagagca tcgccgatga attcttagcc ctgttaaaac
     6601 agcaggcgca aaactggcag ccgggccatc cacttgatcc cgcaaccacc atgggcacct
     6661 taatcgacag cgctcacgct gactcggtac acacctttat tcgtgaaggc
//
LOCUS       DAJTYL010000170         6687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYL010000170 DAJTYL010000000
VERSION     DAJTYL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.170"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1180
                     /locus_tag="OO572_003685"
     CDS             <1..1180
                     /locus_tag="OO572_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6662528.1"
                     /translation="ELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSA
                     EEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAK
                     TLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQ
                     LKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQP
                     GNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYS
                     HWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLG
                     LYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK
                     "
     gene            1232..2389
                     /locus_tag="OO572_003686"
     CDS             1232..2389
                     /locus_tag="OO572_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6662529.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2355..2477)
                     /locus_tag="OO572_003687"
     CDS             complement(2355..2477)
                     /locus_tag="OO572_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662530.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2680..4365
                     /locus_tag="OO572_003688"
                     /pseudo
     CDS             2680..4365
                     /locus_tag="OO572_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4403..>6687
                     /locus_tag="OO572_003689"
     CDS             4403..>6687
                     /locus_tag="OO572_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6662531.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAV"
BASE COUNT         1978 a         1453 c         1414 g         1842 t
ORIGIN      
        1 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
       61 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      121 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      181 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      241 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      301 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctacc
      361 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
      421 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
      481 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
      541 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
      601 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
      661 ttatgacaag ctgatttccg caatggattt ctacccaaca gctcttgatg cagccgatat
      721 cagcattcca aaagacctta agctggatgg tgttcccttg ctgccctggt tgcaagataa
      781 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
      841 tgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
      901 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
      961 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1021 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1081 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1141 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1201 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1261 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1321 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgaagcgtc
     1381 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     1441 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatac
     1501 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     1561 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
     1621 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     1681 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt atcctcttga
     1741 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     1801 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     1861 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     1921 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     1981 ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2041 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2101 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2161 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2221 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2281 ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta agaacagagt
     2341 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     2401 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     2461 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     2521 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     2581 atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag taaatcattg
     2641 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     2701 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     2761 aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg cgtggttaaa
     2821 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     2881 accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat ctttgtatta
     2941 atatccgcaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3001 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3061 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3121 atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3181 tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg tggaacagaa
     3241 tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg tggaacttta
     3301 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     3361 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     3421 aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat taaagagaac
     3481 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat ttattcgcgc
     3541 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     3601 ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa tttaagctgg
     3661 tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     3721 ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca acatgcggta
     3781 caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga gaacttgaac
     3841 tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc gggaaaaacc
     3901 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     3961 gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact gaaagagatt
     4021 atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt actgcatcag
     4081 gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4141 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4201 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg cttactgcgt
     4261 ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4321 tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca ataaaagccc
     4381 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     4441 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat
     4501 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     4561 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     4621 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa
     4681 aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc
     4741 aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     4801 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     4861 ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg
     4921 cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt
     4981 aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac
     5041 ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc
     5101 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5161 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5221 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5281 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     5341 gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact
     5401 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     5461 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt
     5521 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     5581 attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     5641 tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa
     5701 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct
     5761 gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga
     5821 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     5881 ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat
     5941 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6001 aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt
     6061 gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag
     6121 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     6181 caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg
     6241 ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     6301 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     6361 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     6421 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     6481 agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     6541 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     6601 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     6661 caatgtactg gatagcaaaa ccgctgt
//
LOCUS       DAJTYL010000171         6568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYL010000171 DAJTYL010000000
VERSION     DAJTYL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.171"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..481
                     /gene="ydfJ"
                     /locus_tag="OO572_003690"
     CDS             <1..481
                     /gene="ydfJ"
                     /locus_tag="OO572_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6662532.1"
                     /translation="LGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMV
                     ALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCT
                     MTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            570..2030
                     /locus_tag="OO572_003691"
     CDS             570..2030
                     /locus_tag="OO572_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6662533.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2066..2269)
                     /gene="ydfZ"
                     /locus_tag="OO572_003692"
     CDS             complement(2066..2269)
                     /gene="ydfZ"
                     /locus_tag="OO572_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6662534.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2447..3133)
                     /gene="ydfH"
                     /locus_tag="OO572_003693"
     CDS             complement(2447..3133)
                     /gene="ydfH"
                     /locus_tag="OO572_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6662535.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3222..3968)
                     /gene="ydfG"
                     /locus_tag="OO572_003694"
     CDS             complement(3222..3968)
                     /gene="ydfG"
                     /locus_tag="OO572_003694"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6662536.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4105..6150
                     /gene="dcp"
                     /locus_tag="OO572_003695"
     CDS             4105..6150
                     /gene="dcp"
                     /locus_tag="OO572_003695"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6662537.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6195..>6568)
                     /locus_tag="OO572_003696"
     CDS             complement(6195..>6568)
                     /locus_tag="OO572_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6662538.1"
                     /translation="LCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKV
                     AAEMATGAIERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGD
                     CETVLALAVRFALAQLLQLLL"
BASE COUNT         1632 a         1573 c         1685 g         1678 t
ORIGIN      
        1 ccttggttgg ttatccgata aaattggtcg ccggatcccg tatattatta tgaatacctc
       61 tgcgattgtg ctggcgtggc caatgctttc tatcatcgta gataaaagct atgccccgag
      121 caccattatg gttgcactga ttgtgattca taactgtgcg gtgctgggat tatttgctct
      181 ggaaaacatt accatggcag aaatgttcgg ctgtaaaaac cgcttcaccc ggatggctat
      241 ttcaaaagaa attggtggtc ttatcgcttc cggttttggt cctatcctgg cgggtatttt
      301 ctgcaccatg acggaatcct ggtatccgat cgccattatg atcatggcat attcagtgat
      361 tggtttaatc tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga
      421 agacgctgcg gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta
      481 aaccctcaat cccttctctt accggagagg ggatttttat tcatataaca aaacatatag
      541 cttgccatat ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga
      601 cactccctgt ttatgatcgt aataacctgg ccccaagaat tgttcattta ggctttggtg
      661 catttcaccg tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg
      721 actggggata ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac
      781 agcaagataa tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg
      841 ttggcgtcgt taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg
      901 cgatgtgtga accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc
      961 actctccggc gaccggacag ttaatgctcg agcacccgat ggtcgctgcc gacgtgcaaa
     1021 atccccacca gccgaaaaca gcaacagggg tgattgttga ggcgctggct cgccgtaaag
     1081 cggcaggact tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg
     1141 ttatgcgtga cgttgtcact tcctacgcgc aagccgttga tgtaaaactg gcacaatgga
     1201 tcgaagataa cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag
     1261 aggatacgct ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgct ggcgttgcct
     1321 gtgaaccttt ccgccagtgg gttatcgaag ataactttgt agccggacgt ccggaatggg
     1381 aaaaagcggg agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca
     1441 tgctcaacgg cagtcattca ttcctggcat atctgggtta tcttgctgga tatcagcaca
     1501 ttaatgactg tatggaagat gaacattatc gtcatgcggc gtatggcatg atgttgcagg
     1561 aacaagcgcc gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa
     1621 ttgcacgcta tagcaacccg gcgttacgtc atcgaacctg gcagattgcg atggatggca
     1681 gccagaaatt gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca
     1741 agttcgatct gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg
     1801 aacagggaaa tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac
     1861 aaagtagtgc cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg
     1921 gtgatgattt gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt
     1981 tattagcgca tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat
     2041 gccgccatcg gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg
     2101 ccagtttctc ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg
     2161 tcagaccttc agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa
     2221 cacggctgcc agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct
     2281 cctttctgat ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt
     2341 gaaatatttt ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag
     2401 gcggggggaa aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa
     2461 accagtcgct gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt
     2521 gcgtcattgc acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa
     2581 gatgctggcg taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc
     2641 gatcaacggt cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct
     2701 gcgtcaggag ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct
     2761 caatggcaat gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa
     2821 tcatgctcgc cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt
     2881 tgcgcacctg ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa
     2941 tcaggccgtt ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga
     3001 aacgaacaga aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa
     3061 tgtcgcgacg aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg
     3121 tttcgacggt cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt
     3181 tgtgacaagc aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc
     3241 agtccggcat agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca
     3301 ggcagcgttg acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta
     3361 ttttgatagg ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg
     3421 gtgccaccca ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc
     3481 gtacgcagat tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca
     3541 ccggcatacg gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt
     3601 tcaaccatac ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca
     3661 atcatcgttt cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg
     3721 ccggcattat ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc
     3781 tcttcaatag cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt
     3841 tcgtctgtta actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct
     3901 tgttgaataa aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa
     3961 acgatcatct cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta
     4021 agagcgggta atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga
     4081 acacctcgcg ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca
     4141 ctgccgtatc tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc
     4201 gatgagggaa tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg
     4261 cctgatttca acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt
     4321 accagcgtct tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat
     4381 gaacagtttt ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta
     4441 ttcgcgcggg tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc
     4501 atccgcctgg tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa
     4561 gctgataaag caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt
     4621 aaccagcggt tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag
     4681 ctggcaggaa tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt
     4741 ctggataaca aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa
     4801 atgcgcgatc gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa
     4861 aatgatgcca atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag
     4921 gcgaaactgc ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa
     4981 acgccagaag cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg
     5041 agcgatgaat tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg
     5101 cagccgtggg actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat
     5161 gaggcgcagc tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg
     5221 accgcgaatc agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat
     5281 cctgacgttc gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac
     5341 ggtgatttct tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag
     5401 caatcaacgc ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc
     5461 gctgccggtg agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt
     5521 ggtcatacgc tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac
     5581 acgccgcgtg attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg
     5641 caggtattcg ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg
     5701 caacagaaaa tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt
     5761 agcgccgcac ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat
     5821 gtcgatgatt ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg
     5881 ccacgctatc gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat
     5941 tacgcttatc tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag
     6001 ggcggattaa cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac
     6061 agcgaagatc tggaacgcct gcatcgacaa tggcgtggta aggctcctca gattatgccg
     6121 atgctgcaac atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg
     6181 ccaggccagc ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct
     6241 aaagctaata ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct
     6301 ttaatatgcc acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt
     6361 ccgccgtagc cggtaatggc aatactgaca tcagcatccg cacgctctat ggcacctgtt
     6421 gccatttctg ctgccacttt ctcactcacc gcagaatatc gttcaagaga ctgctggctt
     6481 acgctgagga ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat
     6541 ttgggtgtat cttcagctgc acacaggg
//
LOCUS       DAJTYL010000172         6555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYL010000172 DAJTYL010000000
VERSION     DAJTYL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.172"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..475
                     /locus_tag="OO572_003697"
     CDS             <1..475
                     /locus_tag="OO572_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662539.1"
                     /translation="KGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEAA
                     KRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPMT
                     FSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            447..881
                     /locus_tag="OO572_003698"
     CDS             447..881
                     /locus_tag="OO572_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6662540.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1654..2703)
                     /locus_tag="OO572_003699"
     CDS             complement(1654..2703)
                     /locus_tag="OO572_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6662541.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAVFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3154..4344
                     /locus_tag="OO572_003700"
     CDS             3154..4344
                     /locus_tag="OO572_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6662542.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4734..6194)
                     /gene="gtrII"
                     /locus_tag="OO572_003701"
     CDS             complement(4734..6194)
                     /gene="gtrII"
                     /locus_tag="OO572_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6662543.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6191..>6555)
                     /locus_tag="OO572_003702"
     CDS             complement(6191..>6555)
                     /locus_tag="OO572_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6662544.1"
                     /translation="VVEYVRAERVAGISKFNGWKLWNLALEGITSFSTFPLRVWTYIG
                     LFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYI
                     EVKNRPKYIIKKSHRGNP"
BASE COUNT         2073 a         1189 c         1303 g         1990 t
ORIGIN      
        1 gaaggggcgg cacgatcagt tgcttaccgc acttcgcgcg ctgctgttaa gccgcaagaa
       61 tccgtttggc gatatcaaat cggatggcac ggtgaaaacg gctctcgaaa accttggttt
      121 gggagaagca gctaaaagga atgtaggtac aggggcgaat cagatacctg atatgagcct
      181 gttcgcgtca attaataccg taacggctgc tgcgcaaaaa tttccgtctg gattaatttt
      241 acagtgcggt cagttgaatg gtgccccgaa tgtatcttca acatacggga tgaggttccc
      301 gatgacgttc tcaagagtaa ttgctgtcgt agttacattg aacgttactg gcgcggctgg
      361 gcagccgact gtatcggcga caagtgtcca gaacactgga tttaatatta ctgtgtcgcc
      421 cggttcagga tacggttcat ctgctgatgc gtattacatt gcaatgggat attaacaaaa
      481 tgtcatattt ttattctgca tcgacaaacg gattttattc gactgaattt cacggcacca
      541 atattcctga tgatgcagtg gaaatctcgg aatcagagtg gaaaacactg attaatgcac
      601 agagtgtaac aaaaatgatt acctgtggtg agaacggtca tcctgtcatt gttgaccgtc
      661 cttctccaac accagaacaa ttagccttaa taaatgatga aaagaaatct gcactgatag
      721 cagaggcaac gaatgtaata gctccgcttc aggatgcggt tgatttaggt atggcaacag
      781 atgatgaaac gaaactgtta ctggcatgga aaaaatatcg ggtgctgctt atgcgtgtta
      841 atgtagtaaa acccgagtgg cctatgcatc caaataaata gacttgttaa tctaggataa
      901 ttgttggaat aatactcgtt tttcagtagg ttcaggtagc ctgggtataa tgctcacttc
      961 tacaatgatt tcaacttatt ttatttaatt gatattacga aacttaactc atacataaac
     1021 aatatgactc aatactgtcc taaatagctg cgcagaaaag tgctccactt tcgctcagcc
     1081 cttgctgggc gaagcatcag gaaggcgttc tcagctaggt tgtgttatat ctggcttgag
     1141 ttttgttgcc catacgcaac acattaccca tctgtaaacg acaagtgtgg cagaattagg
     1201 tgcagctatt tagactatat cgacgcagta acgataatag atatcgaaac tgtactagat
     1261 attagctact tatggttacc ggggggagtc ttttgagcac tggtgttatc aattggcttc
     1321 aaataattcg ccaataaatt atattgcgat agctcatcac cttccaggtt gtttatagcc
     1381 agtttatcag cgtcaccacc tactgtttat gcttttattt ctttgattaa taagtatatg
     1441 atattttaat tcatctggtg tatttctttc tcatgtgttg gtcgactgaa tatctgttca
     1501 gtgaaggggt aattttatgt ttgcacttcg aacattccct tcagttgcta ggaaagaaaa
     1561 ccgtaacatt tgcgcattaa ttttaacagt ataatctgag cataaatatg cgctatatat
     1621 ctataaaaag gcgcaattaa ttgcgccttt ttatcatttt atcaaaaatg gcccaccgac
     1681 tgtcctcgat agccaatggc caattttatc tgatacatat acacaaatac aggtatatat
     1741 gattgcgcag ataggatttg gaatagtcac gctgaagcct ataattgcta aagccatagg
     1801 gataaataat ttggtgatat actccattcc acatatgtta agagtatttc ttccagctct
     1861 tacaataaag tcatttccac aaattatttt tgctatgaaa atattaaaga ttatcaagcc
     1921 taccgtggtg ataatagtat taatagcttg aatataacta cttgatatta tgtctgcatt
     1981 gaatattgag ataatcttaa aaagtaaagg tttctggtta agtagttgat acgcactcat
     2041 tatagcggta attgagaaga caatgaaacc aattttagat tttccaaaaa acctgtcacg
     2101 tgttaattct aaaaacatac atctaccgag aggcaataac caccagtatg ccatagcgga
     2161 gtctatgttc caaaaccatt gaggatctag taatgggtta tgattgagca ctgtttgtga
     2221 caacatgaac gataacagtg atatgattaa aatcactata ttgttcttga ctatttctct
     2281 aaagattgca tcaattgcta ttataacaaa aaggcagttt ataaaccata ttgttcctac
     2341 gaattggtta tttctaacac catatattat atcataaatg tggttgtata tataatcaat
     2401 cgtctcacct gagttaattg tgtttattat taatatagaa aatgcaaaag tgaaatatgg
     2461 aatcattaat ctgtaaaact ttgacttgat ataatcgaaa acagataagt catttttttt
     2521 gatagtaaaa aaaacagcag cgaaaaagaa tagtgggacg tgataactaa aaacaaaagg
     2581 atacaatttg ccagccccca acccaaggtg acctaaatat atagcaaaaa taccgagaaa
     2641 ttttaaagca tcaacccaat catgtcttat ttttctgttg ctgtcgattt tgttattata
     2701 catttaaagt aggctctcca gttttgtgtg atttttaaac actcattgca aatatgaatg
     2761 cctgaaagtt tatgattatt aatcaacgag ttcaatatta atttattatt tttcaaagtc
     2821 gcactatata ggcatttact tccgcagtac caccgatagt atcgatatca aatcagcctg
     2881 cggccgcgat aactttcgta acattctgcc agcggctttg ttgaataaat caggttcaag
     2941 ataatcgtct ctgcatcagg ttagagtttc aggcaataca tcctctttct ggcatacctg
     3001 ccactctcgc accatggtca tagcctttac gatctgcata ctcaccagac cagagcccgc
     3061 tcccttttag gctgaggaat ccccagaaaa gaagacaggc atagagtttt gtgatctact
     3121 gattgcgcta acaaattcaa tgagatcacc tctatgcctg cccgtaaagt atgccagact
     3181 tttttccgta atgctttagc ttccacgcat cagtatcgac aaaatgccat tattgattct
     3241 gctgctgctt tagtcggtgg cgcatctctt tctcttacca gcattggccg gcatttaccc
     3301 ggtcctgctc gcgtgaaaga taaaataaaa cgcgttgact gtctgttggg taataaacga
     3361 cttcataatg atatccctct gatattcaaa aatattacat ccatgatgac aaataaactg
     3421 tcgtggtgtg ttatcgctgt tgactggagc ggttaccctt ttcaggaata tcatgtcctt
     3481 cgtgccagcc tcctctgcga cggtcgctct atccccctga tgagtcaggt cttcccgtca
     3541 aaaaaaaata acgaggcggt ggaaatcgct tttctggatg cgctttccgg ggccatttct
     3601 ccccggactc gtgttgttat tgttactgat gctggttttc aaagtgcatg gttccgtcat
     3661 atcaaatcac agggatggga ttttattggt cgtatcaggg gggtagtgaa gttcaggctg
     3721 gatagtgaca aagataagtg gctggacata aaaatgtgca gggggagttc agaggcaaaa
     3781 tacctgggga cgggaaccct ggcccggaaa aaacgctcgc agtgtgaagg acatttttat
     3841 ctttataaac attcacctaa aggcagaaaa agcaggcgtg ccagagggag gccgggctta
     3901 ccaacaacgg aaaaagaaca aaaagccgca ggcagagaac catggttaat attcagcaat
     3961 acagctgagt ttaatgccaa aaaaatcatg aaactgtaca gtcgaagaat gcagatagag
     4021 caaaactttc gggatgaaaa aagtgaacgc tttggttttg gccttcgggc gagcagaagt
     4081 cggaggggag agcgttttct ggtattaagt ctactggtta cactggccag tatcgtactc
     4141 tggctacttg gttatcattt tgaaaataaa ggatttcatt taaagtatca ggctaatagt
     4201 ctcaaaaaac gaagagtatt gtcttttctg acgctggctg aaaacgtcct gagatttaac
     4261 cctgaacttc tacgacgagc acaacctgaa aagatattgt gccggttggc cagcacttac
     4321 cgaagtatgg tgctggctta ctgatgaaaa ttttctgggg atccctcagc ctttcgggga
     4381 ggaatgaggg ctctgatatt cttttgccgt agttcatcgt gaaaccgtcg gatgtcattt
     4441 ggcctgagtg acccatgcct cccaaaagca gagtcgctag tgttatgtaa ttaggcggtt
     4501 aaacttcaat tctcagtgtt aagtttgggt aaacgcttgt aaatggtatt gctaattttt
     4561 taattgacta atattagaaa cggttagtgt gattgtatat agatatttga gtgtaaggta
     4621 tttaaagggg cattcagccc ctccgtctat ttgtgattgc atccattgat gtgtgtatta
     4681 ttttgagctt gaaaaaggga ggcgctcttt aataataaat aaatctatca aagctaaata
     4741 ttaaatggaa gccacccttt tttctctgat agttttatta tcaccaaacc atttatttca
     4801 gctatcgaat ctggattcgg ccaggaaggt aaactcttca caattggata aatcttctct
     4861 atttgttctt tatttgctgg tttgcaatta cagattccat agtaattcat aaatgcaacc
     4921 atcctaaaat agttgccacc atcccaccaa aaaaaagatt ttccaaaagt gtctgcgctc
     4981 atggagagca tatttcctga gtcgtttgaa ccatatatat atatatattt tccgttaagg
     5041 gaaatgcctt ttgtttgaga tgtgtatgat atttctttag ctaaagagat atctttctgt
     5101 cttgcgagag tatcattcaa aaatagattt ttggatgttg aaacgccatt aaatataata
     5161 aataaaaaaa acatgcagtt taggtatttt atagagcgaa aattgcttaa gcaagaaaac
     5221 aaaattactg ctactatagg cattaaaaca aacaggcgag gtggcgcccc tgagccaaca
     5281 acaacgataa atataaccgg aagtatataa atgaaaatga ttgatgaaat caaataaatt
     5341 gatcttgttt ttaatttata tgttagggta aacatcagaa ttaataaagg aatcagtaac
     5401 cacttgtata ggtttaaacc agtgggagga ttgttataga agttatatat attatctatg
     5461 gctgatttaa caagccattt aatatcgctt gcattttgta tataatttga gatgtactcg
     5521 ttcgattcaa gcgaaaaata atgcttgata gctttggtta atagcaggta aattaaggta
     5581 gataaagcta tgagggatag agataaacat gaactataaa aagattcacg aatatttttc
     5641 tcatttcgta ttatcgaatt tatctgcaac ccaatgacga atgcaataat atatgttacg
     5701 aatgtttggt agattgccat tgaaagaatg gacagtacta taccagaaaa tatcacaatc
     5761 ctattttttt gcgagtgaaa aataattgct gatatcgctg ccagtagaaa agcaattccc
     5821 acagtatcag cttggttaag aaactctaat tgataggaga tctgagggaa ggtaataaat
     5881 actaacatac ctatcaataa ttcataagat tctagcttta gcgatctgca gattataaat
     5941 gctgaaatga tgataaaaga taaggctatt aatggtgtta tataaagtga aaaaggctca
     6001 gggagaaagt aatgtcgcaa aaaagtgtga aaccaccgcc caagcgtgat tgtttgataa
     6061 aaattatttg taaactcgcc atcaatatta agtgttccat cgtacaacca cccccaataa
     6121 caataaattg atattgcaaa acatgatatg aaggcaagga gatttgcatt tttaaataaa
     6181 ttaattttaa tcatgggtta cctcgatgag atttttttat gatgtatttg ggtctatttt
     6241 ttacttcaat atagattcta cctatatatt ctccgagaac accaatcccg atcagttgca
     6301 ctccacccaa gaaaagtatt gatacaagca gggagggata cccgcgtact gggttaccaa
     6361 agacaagggt gtctataatc atccatgcac catataaaaa tgaaatgctt gcaacaaaca
     6421 agcctatata agtccatacg cgaagaggaa aggttgaaaa acttgtgata ccttccagtg
     6481 ccagattcca taatttccag ccattaaatt ttgagatgcc agcaacacgc tctgcgcgta
     6541 catattcaac gacat
//
LOCUS       DAJTYL010000173         6368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYL010000173 DAJTYL010000000
VERSION     DAJTYL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.173"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..2418
                     /locus_tag="OO572_003703"
     CDS             <1..2418
                     /locus_tag="OO572_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6662545.1"
                     /translation="LSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGKVIVRG
                     KVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPFVFATN
                     GDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDG
                     SGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRIL
                     FLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQ
                     VAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSSTDYFGD
                     PVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFD
                     RTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQVKKND
                     KYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQNIQSM
                     TKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPEDIADPE
                     SDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHVNGVAV
                     GVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQ
                     QGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEAAQAVL
                     DNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSELENEIY
                     QLPPRSA"
     gene            2485..3954
                     /locus_tag="OO572_003704"
     CDS             2485..3954
                     /locus_tag="OO572_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6662546.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3954..5615
                     /locus_tag="OO572_003705"
     CDS             3954..5615
                     /locus_tag="OO572_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6662547.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5836..6177
                     /gene="symE"
                     /locus_tag="OO572_003706"
     CDS             5836..6177
                     /gene="symE"
                     /locus_tag="OO572_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6662548.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6224..>6368)
                     /locus_tag="OO572_003707"
     CDS             complement(6224..>6368)
                     /locus_tag="OO572_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662549.1"
                     /translation="GIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1875 a         1518 c         1478 g         1497 t
ORIGIN      
        1 ctaagtaacc tgacggaagc agacatcatt accaaatgcg ttatgccagc cattctcaat
       61 gcaggctggg acaacacaac gcaaatcaga caggaggtca aactccggga cggtaaagtc
      121 atcgtacgtg gtaaagttgc ggcacgcaga acggtaaaat ctgctgacat cgtgctgtac
      181 cacaagccag gcattccctt agccgtgatt gaagcaaaag ccaacaaaca tgaaattggc
      241 aaagggatgc aacagggcat tgaatatgcg cgcctgctgg acgttccttt tgtttttgcc
      301 accaacggtg atggctttat cttccgcgat gccaccgcag ccgaaggtga atgccttgaa
      361 aagcaaataa cgctggatga cttcccctcc cccgctgaac tctggcaaaa attctgcctc
      421 tggaaaggat acacacaagc tcagcttccg gtgattactc aggactatta cgacgatggc
      481 agcggcaaat cgcctcgtta ttatcaactt caggcaatca acaaaaccat tgaagccgtc
      541 tccaacgggc aaaaccgcgt tctgctggtc atggcgaccg gaacagggaa aacctatacc
      601 gcattccaga tcatctggcg cctgtggaaa tcaaaaaata aaaaacgcat tttgttcctt
      661 gccgatcgca acattctggt cgaccaaacc aaaaataatg atttccagcc atttggtacg
      721 gcaatgacca aagtcagcgg acgcaccatt gatcccgctt atgaaattca cctcgcgctc
      781 tatcaggcta taactggccc ggaggaagac caaaaagcgt ttaaacaagt cgcaccagat
      841 ttcttcgatc tgatcgtgat cgacgaatgc catcgcggca gcgcatccga agacagcgcc
      901 tggcgagaga tccttgatta tttcagttcc gccacccaaa ttggcttaac cgccacgcca
      961 aaagagacgc atgaagtctc cagcacggat tacttcggcg atccggttta cgtctactca
     1021 ctaaaagaag ggatcgaaga cggcttcctc gctccttata aagttgtccg tgttgatatt
     1081 gatgtcgacc tgcaaggctg gcgcccaacc aaagggcaaa ctgacttaaa cggcgaagtg
     1141 attgacgatc gtatctataa ccagaaagat ttcgatcgca cgatggtgat cgacgaacgc
     1201 acagaactgg ttgccagaac cattaccgac tacctcaagc gcaccaatcc gatggataaa
     1261 accatcgtct tctgtaacga catcgatcat gcagaacgta tgcgccgcgc cctggttaat
     1321 ctcaacccgc agcaggtgaa aaagaacgac aaatacgtca tgaaaatcac cggcgatgat
     1381 gaaattggca aagctcagtt ggataacttc atcaacccga aaaaaccgta cccggttatc
     1441 gcgaccactt cagagctgat gaccactggt gtggatgcta aaacctgcaa actggtagta
     1501 ctagaccaga acatccagtc gatgaccaaa ttcaagcaaa ttatcggtcg tggtacacgc
     1561 atcgacgaac gttacggcaa actctggttt accatcctcg actttaaaaa agccaccgaa
     1621 ctgtttgccg atgagcgttt cgatggcatt cccgaaaaag tcatggatac cacaccagag
     1681 gatatcgccg atccagaatc tgattttgaa gagaaactcg aagaaatcag cgaacatgac
     1741 gacgaacagg taacaggcgt tgatgaaccg cctgcgccac cataccaggt taccgatacc
     1801 gatgatgtcg gcccatttcc ggaagaagac gagaagaaaa tccgcaagtt tcacgtcaac
     1861 ggtgtagcgg tgggcgttat tgcacagcgc gttcagtatt acgacgccga cggtaaactg
     1921 gttaccgaat cctttaaaga ttacacccgc aaaacactgc tcaaagaata tgcctcgctg
     1981 gatgacttta cccgcaagtg gcaggacgcc gatcgcaaag aagcgatcat tcacgagctg
     2041 gagcaacagg ggatcatctg ggaagtactg gcagaagaag tcggtaaaga tctcgacccg
     2101 ttcgacatgc tttgccacgt agtgtatggt cagccgccgt taacccgcaa agagcgcgcc
     2161 gagaacgtgc acaagcggaa ctacttcaca aaatactctg aagcagcgca agccgtgctc
     2221 gataatctgc tggataaata cgccgatgcg ggcgtacagg agatcgaaag tattcaggtg
     2281 ctgaaactta aaccattcga cagcatgggc accttaccgg agattattaa aaccggattt
     2341 ggcgaccgta acgggtataa tcaggcgctc agcgagctgg aaaacgaaat ctaccaatta
     2401 ccgccccgct ctgcttaaca actctctttt gcgccttctg caaccatcat gttgcagaag
     2461 gccttgtttt tcatggaata caacatgtct atcagctcag taatcaaatc attacaagat
     2521 attatgcgca aagatgccgg tgtggacggc gatgcgcagc gtctcggtca gctctcctgg
     2581 ctgctgtttt tgaaaatctt cgatgcccag gaagaggcgc tggaactgga gcaggataac
     2641 tatcaatatc cgatcccaca gcgttatttg tggcgcagtt gggccgccaa cgcgcagggc
     2701 attaccggtg attccctgct ggaattcgtt aatgatgatc tgtttccggc gttgaaaaac
     2761 ctcactgcgc ctatcgataa aaacccacgc ggctacgtgg tgaagcaggc gttcagcgat
     2821 gcctataact atatgaaaaa cggtacgcta ctgcgccagg tgatcaacaa gctgaacgaa
     2881 attgacttta ccagcgccag cgaacgccat ctgtttggtg atatttacga acagatcctt
     2941 aaagatctgc aatctgcggg caatgcgggc gaattctata ctccacgcgc cgtcactcgc
     3001 tttatggtgg atcgcgttga tccgaaactc ggcgaatcca ttatggaccc ggcctgcggt
     3061 acgggcggtt ttcttgcctg cgcgttcgat catgtaaaga acaaatacgt gaagagcgtc
     3121 gccgatcatc agacactgca acaacagatc cacggtgttg agaaaaaaca gcttccgcac
     3181 ctgctggcga ccaccaatat gctgctgcac ggcattgaag taccggtaca aattcgtcac
     3241 gacaacaccc tgaataaacc gctttcctcc tgggatgagc aactggatgt cattgttacc
     3301 aacccgccgt ttggtggcac ggaagaagac ggtattgaga agaactttcc ggcagagatg
     3361 caaacccgcg aaacggcgga tctgtttctg caactgattg tggaagtact ggcgaaaaac
     3421 ggtcgtgcgg cggtggtatt gccggatggc acactatttg gcgaaggtgt taaaaccaaa
     3481 atcaaaaagc tacttaccga agagtgcaac ctgcatacca tcgtgcgttt accgaatggt
     3541 gtgtttaatc cctataccgg cattaaaacc aacctgttgt tctttaccaa aggtcagcca
     3601 accaaagaga tttggttcta tgaacatccg tatccggcgg gcgtgaaaaa ctacagcaaa
     3661 accaagccga tgaagtttga agagtttcag gcggagatcg actggtgggg taacgaggcc
     3721 gatggttttg ccagccgcgt cgagaatgag caagcgtgga aagtcagcat tgatgacgtg
     3781 attgcgcgca acttcaatct ggatattaaa aacccacatc aggcggaaac cgtcagccat
     3841 gatccggacg aactgttagc gcagtatgca aaacagcagg cggagatcca gacgctgcgt
     3901 aatcaactgc gcgatattct tggcgctgcg ctgtctggca aggaggttaa ctaatgagtg
     3961 tggaaaagct gatcgttgat catatggaaa cctggacctc ggcgttgcaa acccgttcca
     4021 ccgccgggcg cggcagctcc ggtaagattg atttgtatgg tattaagaaa ttgcgtgagc
     4081 tgattctgga actggcggtg cgcggtaaac tggtgccgca ggatccgaat gatgaaccgg
     4141 cgtcggacct gctgaagcgt attgcggcgg aaaaagcaga gctggtgaag caaggcaaaa
     4201 ttaaaaagca aaaaccactg ccagaaatta gtgaggaaga gaagccgttt gaattgccgg
     4261 agggatggga gtgggtgcac ctccctgata tttattgctc aatttctgag tcatctagaa
     4321 aaataaaatc ctccgaaata ctacctgagg gaaaatatcc tgttatcgag caaagccaag
     4381 aatttatttc tggttattgc aacaatgaat gtctgttaat aaagttaaat aatccagtta
     4441 tagtttttgg tgatcacaca aggaatatta aatttattga ttttgatttt gttgtcggcg
     4501 ctgacggtgt gaagatttta tcacctatat taatttgtga gcgtttcttt ttttggcagt
     4561 tacgatcatt taaattagat gtaagaggat acgcgagaca ttttaaagta ttaaatagct
     4621 gcttatttgc attacccccc attgcagaac aagagcgaat agttgaaaaa gttagctctt
     4681 tgatgtccct ctgcgaccaa ctagaacagc aatccctgac cagtctggac gcacatcagc
     4741 aactggttga aaccctgttg ggaacgctta cagacagcca aaacaccgcg gaactggctg
     4801 aaaactgggc gcgtattagc gagcatttcg acaccctatt taccaccgaa gccagcgtgg
     4861 atgcgttaaa acagaccatt ctgcaactgg ccgtaatggg caaacttgtg ccgcaggatc
     4921 cgaatgacga acccgcctct gaactgctca aacgtattgc gcaggaaaaa gctcaattgg
     4981 tgaaagaagg gaaaataaaa aaacaaaaac cactgccgcc aattagcgat gaggaaaaac
     5041 cgtttgaact tccggaaggg tgggaatggt gtcgtattgg taatatagtt aatattaaat
     5101 ctgaattagt atcaccaaaa gattatttaa atttatatca agtagcacct gatattattg
     5161 aaaagggcac tgggagagtg atatcaaaga gaacagtgaa agaatcagga gtcaaagggc
     5221 caaatagtcg cttttataaa ggacaaattg tttattctaa aatccgcccg tcattatcca
     5281 aagttttttt agcagagtat aatggtcttt gcagtgctga tatgtatcca ttagactgtt
     5341 acattaatcc aaattactta ttgaaatata tcctttccat tccatttctt atgcaagtca
     5401 aaaaagcaga gaacagaata aaaatgccaa aacttaattc tgacagtttt tacaatatta
     5461 ttgttgcgat tcccccctat aatgaacaac aagcaatatt tgataagata aattctattg
     5521 aagcagtatg caatggtctt atttcttata ttgggattta tcataaaacc caactgcatc
     5581 ttgcagacgc actcactgac gcagcgataa actaattccc tcgccctcac aaaaccaata
     5641 aagcggctaa atagccgctt tattcacatc accaaaattt atatttcacg ctaattttca
     5701 tcttattttc attaattttt agctggactt tccctgtatt tactgatgat gtatccaggt
     5761 atttagcacg gtgcggatgt gcgccaacac actcgcaccg ctaatcacaa tcactattta
     5821 tagagaatag taatcatgac tgacacgcat tctattgcac aaccgttaga agcagaagtc
     5881 tccccggcaa ataatcgtca attaaccgtg agttatgcga gtcgttaccc ggattacagc
     5941 cgtattcccg ccatcaccct gaaaggtcag tggctggaag ccgccggttt tgccaccggc
     6001 acggcggtag atgtcaaagt gatggaaggc tgtattgtcc tcaccgccca acccgccgcc
     6061 gccgaagaga gcgaactgat gcagtcacta cgcaaggtgt gtaagctgtc ggcgcgtaaa
     6121 caaaggcagg tacaggaatt tattggggta attacaggta aacagaaagt cgcctgatac
     6181 attgacccgg tagcgccagt gctaccgggt attattcgcc gcgttatacc aacgcgctct
     6241 ccatctgttt ttcaaatgcc tgacgaatta atcccataat gtaaggaagt tgtgcgaggt
     6301 cgctgaaacc aatttctaca tcgccattac cccagcgccc aacatttgtc acatctttgg
     6361 caatacct
//
LOCUS       DAJTYL010000174         6366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYL010000174 DAJTYL010000000
VERSION     DAJTYL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.174"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(106..1815)
                     /locus_tag="OO572_003708"
     CDS             complement(106..1815)
                     /locus_tag="OO572_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6662550.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO572_003709"
     CDS             complement(2247..2966)
                     /locus_tag="OO572_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6662551.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="OO572_003710"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="OO572_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="OO572_003711"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="OO572_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="OO572_003712"
     CDS             complement(3998..4576)
                     /locus_tag="OO572_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662552.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5234)
                     /locus_tag="OO572_003713"
                     /pseudo
     CDS             complement(4997..5234)
                     /locus_tag="OO572_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5421..5850)
                     /locus_tag="OO572_003714"
                     /pseudo
     CDS             complement(5421..5850)
                     /locus_tag="OO572_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5898..6263)
                     /locus_tag="OO572_003715"
     CDS             complement(5898..6263)
                     /locus_tag="OO572_003715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662553.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT         1763 a         1445 c         1143 g         2015 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccttcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     5281 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     5341 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     5401 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     5461 ataacctgct ctgccagacg gtaacccctc ttcagccccg taaaccgcat ctgacctccg
     5521 cgcagctcgc acttcagcgg gtcaaccttc agacactgct gatacatccc tctccttgtg
     5581 atttgcctcg ccgtttttct cactgtctcc gttatggcgt acaccacatc ttccagtaat
     5641 cgccgcttca ccggactcag gaccccgtat caccatccgg aaccccttat ccgccacatg
     5701 ccaggagaac ctttccataa actcgtcgcc actcatcaca aggttttctt ctcgttttgt
     5761 ccggtggctg ttgtaacgca ggccaatttc atcctgaccg gcataatgtt ccaggcggct
     5821 catctgcacc ggctgcttct ccagccagtt aagccatcgg tttccctgct gaaaaatatc
     5881 ggcaaaacgg ggaagcatca gaaaggcgga ataacgccat ccggccagtg aaccgtaccg
     5941 cattcagggc aatacatgcc gtcggcgttg ataccggcag gaattgtcgc aaattcccgc
     6001 tccgtgcagc gggcaatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
     6061 agttttttgt ttccggcatc cacaaagaac ggaatattca ggtctgcgca acactcaacg
     6121 gcatcgtcaa aattatcaaa gcgcagaacc tcagcttctt cttcgtcaaa aaaatcatct
     6181 tcgtgaagtt ttacgacata gcggaggagt ttgcttcttt gaggtgcagg ttttcgtttg
     6241 cgtggtttag ctgaacgggc catataacta cctgaaagac aatgatattg tctgttttta
     6301 taacggtaat cacaggccat gacaagccgc agccgtcagg ccgcctactc ggggcggttt
     6361 agaacg
//
LOCUS       DAJTYL010000175         6027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYL010000175 DAJTYL010000000
VERSION     DAJTYL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.175"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO572_003716"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO572_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6662554.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO572_003717"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO572_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6662555.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO572_003718"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO572_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..>6027)
                     /locus_tag="OO572_003719"
     CDS             complement(5797..>6027)
                     /locus_tag="OO572_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HCR6662556.1"
                     /translation="THNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGD
                     RVIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1578 a         1421 c         1430 g         1598 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggt
//
LOCUS       DAJTYL010000176         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYL010000176 DAJTYL010000000
VERSION     DAJTYL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.176"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..393)
                     /locus_tag="OO572_003720"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO572_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO572_003721"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO572_003721"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6662557.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO572_003722"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO572_003722"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6662558.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO572_003723"
     CDS             5797..>5983
                     /locus_tag="OO572_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6662559.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTYL010000177         5739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYL010000177 DAJTYL010000000
VERSION     DAJTYL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.177"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            127..1035
                     /locus_tag="OO572_003724"
     CDS             127..1035
                     /locus_tag="OO572_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6662560.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1081..3072)
                     /gene="tkt"
                     /locus_tag="OO572_003725"
     CDS             complement(1081..3072)
                     /gene="tkt"
                     /locus_tag="OO572_003725"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6662561.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFEAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3350..4108
                     /gene="loiP"
                     /locus_tag="OO572_003726"
                     /gene_synonym="yggG"
     CDS             3350..4108
                     /gene="loiP"
                     /locus_tag="OO572_003726"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6662562.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4314..5234)
                     /gene="speB"
                     /locus_tag="OO572_003727"
     CDS             complement(4314..5234)
                     /gene="speB"
                     /locus_tag="OO572_003727"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6662563.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5370..>5739)
                     /locus_tag="OO572_003728"
     CDS             complement(5370..>5739)
                     /locus_tag="OO572_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662564.1"
                     /translation="YQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVNAGD
                     SLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPP
                     CSGQAFPADNNGYAPPPCRK"
BASE COUNT         1380 a         1530 c         1513 g         1316 t
ORIGIN      
        1 aaacatactt ttctaatgca atgttttttc gctatacccc tttatagatt ctgaattctc
       61 acctgtcggc tgttttctcc tcatctcatg tgacatattc ccatacttta ctcaaggaag
      121 attgtcatga aagcacctgt ttcattaacc tcatttcgcc cacaaaaatc tcttgctatc
      181 gccattggcg tgcttgccgt ggttgtgctg ccatttctct cgtattacac cgttaatgaa
      241 ggtgaacgcg gcattttgct gcgctacgga aaaatcgtaa aagttgccga accggggctg
      301 ggttttaaga tcccttttat ggaaagcgtg gagaaaattt ccacgcgtaa tcaggctgtg
      361 gtctatcagg gattacaggc ctacagccgc gaccagcaac cggcacaaat gacagtatca
      421 gtcagcttcc acattaaacc ttcagaagca ggcgcggttt ataccaccta caacactatt
      481 gaagcattaa aagatcgtct aattgtccgt cagttgccaa ctcagctgga gaacattttc
      541 ggccaatata ccgccatcag cgccgtacag gatcgtacaa aattggttca ggatctgcaa
      601 aatgccatgc gcaaagccgt tgttgggcca gtggtaattg acggggtcca gattgaaaat
      661 attgattttt ctgacgctta tgaaaagtcg attgaaaacc gaatgaaagc agaagtcgcc
      721 attgccacgc gtaagcaaaa tctggaaaca gagaaaatcc aggcgcaaat agcggtaact
      781 caggcccagg cagaagcaga cagtaaactg gcggcagcca aagcagaagc ggaaactatt
      841 cgcgtaagag gcgcggcaga ggctgaaact attcgtttaa aaagtgcggc tgaagcagaa
      901 gcaatccgcc tgcgcggtga agctctgcgg gataatcccg gacttgtagc cctcaccact
      961 gccgaacgtt gggacggtaa actgcccgat accatgatcc ccggcagcac agttccgttt
     1021 atctctacta agtaaaaaaa gggccgcttt cgcggtcctt ctcacaaaac gtcttactga
     1081 ttacagcagc gcttttgctt tcgcaacaac gttatcaaca gtgaagccga actcttcaaa
     1141 cagcagctct gccggagcag attcaccgaa ggtggtcatg ccgacgatag cgccgttcag
     1201 gccaacatac ttgtaccagt agtcagcaat acccgcttct accgcaacgc gtgcagtaac
     1261 cgctttcggc agtacggatt cacggtaagc cgcatcctgc ttgtcaaatg cgtcggtaga
     1321 cggcatggac accacgcgcg ctttcacgcc ttcggcagtc agtttttcgt aggcagcaac
     1381 cgccagttca acttcagaac cggtggcgat gaagatcagt tccggctgac cagcacagtc
     1441 tttcagcaca taaccaccgc gcgcgatgtt tgccagctgc tcttcagtac gttcctgctg
     1501 cgccaggttc tgacgggaga ggataagcgc agtcgggccg tcctgacgct caacgccgta
     1561 tttccacgcg accgcggatt caacctggtc acacggacgc catgtagaca tgttcggggt
     1621 cacgcgcaga gaagcgacct gctctaccgg ctggtgagtc gggccatctt cgcccagacc
     1681 gatggagtcg tgggtgtaaa ccatcacctg gcgctgtttc atcagcgcag ccatacgtac
     1741 ggcgttacgt gcgtattcca cgaacatcag gaaggtggag gtgtacggca ggaagccacc
     1801 gtgcagggag ataccgttag caatcgcggt cataccgaac tcgcgaacac cgtagtggat
     1861 gtagttacct gcagcatctt cgttgattgc tttagaacca gaccacaggg tcaggttaga
     1921 cggtgccagg tcagcagagc cgccgaggaa ttcaggcaac agcgggccga acgcttcgat
     1981 agcattctgc gacgctttac ggctggcgat tttcgccgga ttagcctgca gtttagcgat
     2041 aaactctttc gctttggcgt cgaagtcaga cggcatttcg cctttcatac ggcgggtaaa
     2101 ttcagccgct tcctgcggat aagctttcgc gtaagcctca aacttctcat tccatgcaga
     2161 ttctttcgcc tggcctgctt ctttcgcatc ccactgagca tagatttcag acgggatttc
     2221 gaacggtgca tatttccagc ccagctgttc gcgggtcagg gcaatttcag cgtcgcccag
     2281 cggcgcaccg tgggagtcgt gagtaccggc tttgttcggg gaaccgaaac cgatgatggt
     2341 tttgcacatc agcagggacg gtttgtcagt cactgcgcgc gcttcttcaa ctgcgcgttt
     2401 gatggatgcc gcgtcatgac cgtcgatgtc gcgaataacg tgccagccgt aagcttcgaa
     2461 acgcattgct gtgtcgtcgg tgaaccagcc ttcaacgtga ccatcgatag aaataccgtt
     2521 gtcatcgtag aacgcaatca gtttacccag cttcagcgta cccgccagag agcaaacttc
     2581 gtgggagatg ccttccatca tgcagccgtc gcccatgaag gcgtaggtgt agtggtcgac
     2641 aatgtcgtgg cccggacggt taaactgcgc cgccagcgtt ttttctgcaa tcgccatacc
     2701 gactgcgttg gcaataccct gacccagcgg accggtggtg gtttccacac cagcggtata
     2761 acctacttcc ggatggcccg gagttttaga gtgcagctga cggaagtttt tcagttcttc
     2821 catcggcaga tcgtaaccgg tgaggtgcag caggctgtag atcagcatgg agccgtggcc
     2881 gttggacagc acgaagcggt cacggtcagc ccaggacgga ttctgcgggt tgtgtttcag
     2941 gaaatcacgc cacaggactt cggcaatgtc agccataccc ataggggcac ccgggtgacc
     3001 ggatttggct ttctgtactg cgtccatgct cagcgcacga atagcattgg caagctcttt
     3061 acgtgaggac attttgactc cagatcggat gatgaagggc acgcccttaa cgacttgacg
     3121 acagcgcgtt ttgggctacg ccggaaaatt tgccaacaat ttaccgcaag ccgcgcgtca
     3181 tgtacatgga acatcctttt gccgcttcag aaatctctgg atcatgctcg catgttgcgc
     3241 aatctgctcg cccgtccgct gcgcttttcc ttatactgag actgagcgtc gattcacctg
     3301 caaacggcgc atttttagaa taatcctgac cttgtgcgga agagaaaaca tgaaaattcg
     3361 cgccttattg gtagcaatga gcgtggcaac ggtactgacc ggttgccaga atatggactc
     3421 caacggactg ctctcatcag gagcggaagc ttttcaggct tacagtttga gtgatgcgca
     3481 ggtgaaaacc ctgagcgatc aggcatgtca ggagatggac agcaaggcga cgattgcgcc
     3541 agccaatagc gaatacgcta aacgtctgac aactattgcc aatgcgctag gcaacaatat
     3601 caacggtcag ccggtgaatt acaaagtgta tatggcgaag gatgtgaacg cctttgcaat
     3661 ggctaacggc tgtatccgcg tctatagcgg gctgatggat atgatgacag ataacgaagt
     3721 cgaagcggtg atcggtcacg agatggggca cgtggcgtta ggccatgtga agaaagggat
     3781 gcaggtggcg cttggtacaa atgccgtgcg agtagctgcg gcctctgcgg gcgggattgt
     3841 cggcagttta tctcaatcac aacttggtga tctgggcgag aaattagtca attcgcaatt
     3901 ctcccagcgc caggaagcag aagccgatga ttattcttac gatcttctgc accaacgcgg
     3961 catcagcccg gcaggtcttg ccaccagctt tgaaaaactg gcgaagctgg aagaaggtcg
     4021 tcaaagctca atgtttgacg accatcctgc atccgccgaa cgtgcccagc atattcgcga
     4081 tcgcatcagc gcggatggga ttaagtaaag cctagtggtg tcgaacgtag gtcagataag
     4141 gcgttcacgc cgcatctgac attaatggca catttgttac cttgtgcgca ttgccggatg
     4201 cgatgctggc gcatcttatc cggcctacgg gtgccgaacg taggtcggat aaggcgttca
     4261 cgccgcatcc gacattaatg gcacgtttta cccgtgcgca tcgcatctgg tgcttactcg
     4321 ccctttttcg ccgcctgaat atacagcatc tccagcgcca gcgtcgccgc agccagagcg
     4381 gtgatttccg actgatcgta tgccggagcc acttccacta cgtccatccc aacgatgttg
     4441 agatctttca ggccgcgtac cagtttaatg gcgcgatcgg aggtcaggcc gccaatcact
     4501 ggcgtaccgg ttcccggcgc gaaagcagga tccaggcagt cgatatcaaa agtcaggtaa
     4561 accggcatat cacccacaat ctgtttcacc tgggcgataa cgtcatccac gctgcgatcg
     4621 ttcacctggc aggcgtccag cacggtaaag ccgttgtctt tatcaaactc ggtacgaata
     4681 ccaatctgca cggaatgatt cgggtcgatc agaccttctt tcggcgcggt atagaacata
     4741 gtgccgtggt caaattcaca accgttcgca taggtatcgg tgtgggcgtc aaagtgtacc
     4801 agcgccattt tgccgaaatg cttcgcatga gcacgcagca gcggcagcgt aacaaagtgg
     4861 tcaccaccga aggagagcat acgcttaccg gcagccagca gcttctcggc gtgcgcctgc
     4921 agcttttcgc tcatctcacg ggcatcgcca aaggcatata ccagatcgcc gcagtccacg
     4981 acgttcagac gctcacgcat gtcgaaattc cacgggaagc ggttgtgttc ccaggccaga
     5041 ttcgtcgaaa cctgacggat cgctgccgga ccgtggcgac cacccgcacg accagaagtg
     5101 gccatatcga acggcacgcc agtaatcacc cagtctgcat cgctgtcata cggctggaag
     5161 ttcatcggca ggcgtaaaaa accaaaggca ttggaaacca gtgagttatc gtattgatga
     5221 cctaaggtgc tcatggatgg actcctatta caaagtcgat ataaaaaaac ccttccgcgt
     5281 cgttaggccc gacgaggaag ggttggattt gcgaaacaca agtttgtgct aattatcgcc
     5341 ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac acggtggcgg cgcatagcca
     5401 ttgttatctg ccggaaaggc ttgtccagag cacggcggca gcgcgttttg cgccgggcct
     5461 tgtaattcaa aggcggcaaa gccgattacc ccggtattac gcacatcgcc ttgcgccgaa
     5521 ttggctgcgt aggaatcagc aacgttggcg aactgaaaag ccgcttcagt atgtttatct
     5581 ttgcggaatc ctttaatcac taacgaatct ccggcattca caatatagcc gttgttattc
     5641 agcgaacctt gcttgccgtt cagcacatcc aggccatcag ccgtagcaac gatctcgtag
     5701 ttggtgttac gactgtaatt acggacatat aattgataa
//
LOCUS       DAJTYL010000178         5734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYL010000178 DAJTYL010000000
VERSION     DAJTYL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.178"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..163
                     /locus_tag="OO572_003729"
                     /pseudo
     CDS             1..163
                     /locus_tag="OO572_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            688..1866
                     /gene="ltrA"
                     /locus_tag="OO572_003730"
     CDS             688..1866
                     /gene="ltrA"
                     /locus_tag="OO572_003730"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6662565.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1949..2128
                     /locus_tag="OO572_003731"
                     /pseudo
     CDS             1949..2128
                     /locus_tag="OO572_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2158..2640)
                     /locus_tag="OO572_003732"
                     /pseudo
     CDS             complement(2158..2640)
                     /locus_tag="OO572_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2896..3876)
                     /locus_tag="OO572_003733"
     CDS             complement(2896..3876)
                     /locus_tag="OO572_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6662566.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3876..5075)
                     /locus_tag="OO572_003734"
     CDS             complement(3876..5075)
                     /locus_tag="OO572_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6662567.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5397..>5734)
                     /locus_tag="OO572_003735"
     CDS             complement(5397..>5734)
                     /locus_tag="OO572_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6662568.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1572 a         1315 c         1365 g         1482 t
ORIGIN      
        1 gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta aagtccatgc
       61 cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga ctgcccggct
      121 gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat aagttatcca
      181 tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca cagaccaaac
      241 gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa aaggcagaga
      301 tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa
      361 atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc
      421 tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc
      481 agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag
      541 tagtgatgaa agatctgtaa tgggtctgga gctaagggat ggggatcaca ttcattgcag
      601 aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa
      661 aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc
      721 gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg
      781 aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag
      841 tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga
      901 gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt
      961 taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt
     1021 tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg
     1081 gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc
     1141 tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc
     1201 actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt
     1261 gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt
     1321 gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc
     1381 ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg
     1441 tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt
     1501 acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca
     1561 gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag
     1621 agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac
     1681 ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg
     1741 ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg
     1801 ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat
     1861 gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga
     1921 gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc
     1981 ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct
     2041 gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc
     2101 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt
     2161 ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg
     2221 aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt
     2281 ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca
     2341 ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt
     2401 aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca
     2461 gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca
     2521 gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat
     2581 actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat
     2641 ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg aaggctgccg
     2701 tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt
     2761 ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa
     2821 acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg agttgctggc
     2881 gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg
     2941 atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc
     3001 ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac
     3061 cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat
     3121 aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact
     3181 cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact
     3241 taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga
     3301 cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa
     3361 tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg
     3421 caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg
     3481 agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag
     3541 caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat
     3601 cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc
     3661 cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtatc
     3721 cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt
     3781 aaatacatga actggagccg atatttcttc agtattattc aggatatttg tattcagcgt
     3841 acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca
     3901 atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct
     3961 tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg
     4021 cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc
     4081 tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag
     4141 ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca
     4201 ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt
     4261 gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct
     4321 gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat
     4381 atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca
     4441 ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg
     4501 ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt
     4561 gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga
     4621 tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg
     4681 gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg
     4741 aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag
     4801 atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt
     4861 tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt
     4921 aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga
     4981 aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc
     5041 tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt
     5101 taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat
     5161 gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta
     5221 agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac
     5281 cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt
     5341 ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag
     5401 tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc
     5461 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta
     5521 cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg
     5581 gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct
     5641 aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca
     5701 gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAJTYL010000179         5516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYL010000179 DAJTYL010000000
VERSION     DAJTYL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.180"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1028
                     /gene="narU"
                     /locus_tag="OO572_003736"
     CDS             <1..1028
                     /gene="narU"
                     /locus_tag="OO572_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6662569.1"
                     /translation="QNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSAL
                     GINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAI
                     ATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKT
                     QFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPG
                     TGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAV
                     TETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYG
                     RRKFSQK"
     gene            1110..4850
                     /gene="narZ"
                     /locus_tag="OO572_003737"
                     /pseudo
     CDS             1110..4850
                     /gene="narZ"
                     /locus_tag="OO572_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            4847..>5516
                     /gene="narH"
                     /locus_tag="OO572_003738"
     CDS             4847..>5516
                     /gene="narH"
                     /locus_tag="OO572_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6662570.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLC"
BASE COUNT         1290 a         1485 c         1497 g         1244 t
ORIGIN      
        1 tgcaaaatcc gaatactcct tttgggatat ttatcgttat cgctttgcta tgcggttttg
       61 caggtgcaaa ctttgcttcg agcatgggca atatcagttt cttctttcca aaagccaaac
      121 aagggagcgc tcttgggatt aatggcggat taggaaactt aggtgtaagt gtaatgcagc
      181 tggttgcacc gctggtcatt tttgtacctg tatttgcctt tctcggcgtc aatggcgtac
      241 cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc atggatttgg gtaccgctac
      301 tggcgattgc cacgatcgcc gcctggtcag ggatgaatga tatcgccagt tcacgcgcct
      361 caattgccga ccagctccct gtcttacaac gcctgcatct ctggctgctg agcctgcttt
      421 accttgccac cttcggttcg tttatcggtt tttctgcggg ttttgccatg ctggcaaaaa
      481 cccagttccc ggatgtgaat attctgcgcc tggcgttctt tggcccattt attggtgcca
      541 tcgcgcggtc ggttggtggt gctatttccg ataaattcgg cggcgtgcgg gtgacgttga
      601 tcaactttat ttttatggcg attttcagtg ccctgctgtt ccttacctta ccgggcacag
      661 gctccggtaa tttcatcgcc ttttacgccg tatttatggg gctgtttctg accgcgggtc
      721 tgggaagtgg ttctactttc cagatgatcg ccgtcatctt tcgccagata accatttatc
      781 gggtgaagat gaaaggcggt agtgatgagc aagctcagag agaagccgtc accgaaacgg
      841 cggcaacgct gggctttatc tcagccattg gcgcagtggg cggctttttt attccgcagg
      901 catttggcat gtcgctcaat atgaccggct ctccggtcgg cgcgatgaaa gtgtttttaa
      961 tcttctacat cgtttgtgtg ctgctgacct ggctggttta tggtcggcgg aagttcagcc
     1021 aaaaataaga gatcgatgcc tgatgtacaa ccatgtcgca tccgacaagg catcagatcc
     1081 caattcattc ctggagcagg agtcatgtca tgagtaaact tttggatcgc tttcgctact
     1141 tcaaacaaaa gggcgaaacc tttgccgatg gtcacggaca ggtgatgcat agcaaccgcg
     1201 actgggagga cagctatcgc cagcgttggc agttcgacaa aatcgtgcgt tccacccacg
     1261 gcgttaactg tacaggctcc tgtagctgga aaatctacgt taaaaatggt ctggtgacct
     1321 gggaaatcca acagaccgac tacccgcgca ctcgccctga cctgcccaat catgaacctc
     1381 gcggctgccc gcgtggcgca agttactcct ggtatcttta cagcgctaac cgcctgaaat
     1441 acccgctcat tcgtaaacga ctgattgaac tgtggcgcga agccctcaaa caacacagcg
     1501 atccggtact ggcgtgggca tcgattatga atgatccgca aaagtgcctg agctacaaac
     1561 aagtgcgtgg gcgcggcggg tttatccgct ccaactggca ggaactaaac cagctgattg
     1621 ccgccgctaa cgtctggacc atcaaaacct acggcccgga tcgcgttgcc ggtttctcgc
     1681 cgatcccggc gatgtcgatg gtttcttacg ccgccggaac gcgttatctg tcgctgcttg
     1741 gcggcacctg ttaaagtttc tacgactggt attgcgacct gccgcccgcc tcgccgatga
     1801 cctggggcga gcaaaccgac gtaccggaat ctgccgactg gtataactcc agctacatca
     1861 tcgcttgggg gtctaacgta ccgcagacac gtacgccgga cgcccacttc tttaccgaag
     1921 tacgctacaa aggcactaaa accatcgcca ttacccctga ctactctgaa gtggccaaat
     1981 tgtgcgacca gtggctggca ccgaaacaag gcactgatag cgccctggcg atggcaatgg
     2041 ggcatgtgat tttaaaagag tttcatctcg ataatcccag cgactacttt atcaactact
     2101 gccgccgcta cagcgacatg ccgatgctgg taatgctgga gcctcgcgac gatggtagct
     2161 acgttcccgg gcgcatgatc cgcgcatctg acctggtgga tggactgggc gaaagcaaca
     2221 atccgcagtg gaaaaccgta gcagttaata ccgcaggtga attggtagtg ccgaacggtt
     2281 cgattggttt ccgctgggga gaaaaaggca aatggaatct ggaatccatt gccgccggta
     2341 cggaaaccga attgtcgtta accctgctcg gtcaacatga cgctgttgca ggcgtggcct
     2401 tcccctactt tggcggcatt gaaaatccgc attttcgcag cgtaaaacac aatccggtgc
     2461 tggtgcgcca attgcccgtt aaaaacctga cgttagtcga tggcaacacc tgtccggtgg
     2521 tcagcgttta tgatttggta ctggcgaatt acggcctcga tcgcgggctg gaagatgaaa
     2581 acagcgcgaa agattacgct gaaatcaaac cgtacacccc agcctggggt gagcaaatta
     2641 ccggcgtacc gcgccagtat attgaaacca tcgcccgcga atttgccgat accgcccata
     2701 aaacgcatgg gcgctcaatg attatcctcg gcgcaggtgt taaccactgg tatcacatgg
     2761 acatgaacta ccgcgggatg atcaatatgc tgatcttctg cggttgtgtc gggcaaagcg
     2821 gtggcggctg ggcacactat gtcggccagg aaaaactgcg cccacaaacc ggctggttgc
     2881 cactggcctt tgcgctcgac tggaaccgac caccgcgcca gatgaacagc acctcgtttt
     2941 tctacaatca ttccagccaa tggcgctatg aaaaagtctc tgctcaggag ttactttcac
     3001 cgctcgccga tgccagtaag tacagcggtc atctgattga tttcaacgtt cgcgccgaac
     3061 gtatgggctg gctaccttct gcgccgcagc tggggcgtaa cccgctcggg attaaagctg
     3121 aagccgacaa ggccggatta tcccccacag aatttaccgc ccaggcgctg aaatcgggcg
     3181 atttacgtat ggcctgcgaa caaccagata gcggcagcaa tcatccgcgt aatttgtttg
     3241 tctggcgctc taacctgctt gactcctccg gcaaaggcca cgagtatata cagaagtatc
     3301 tgctggggac cgaaagcggg attcagggcg aggaactcgg tgccagcgac gggatcaaac
     3361 cggaagaagt cgagtggcaa actgcggcga ttgaaggcaa gctcgacctg ctggtgacgc
     3421 tcgacttccg catgtccagt acctgcctgt tctccgatat cgttctgccc accgccacct
     3481 ggtatgaaaa agacgatatg aacacctcgg atatgcatcc atttattcat ccgctttctg
     3541 cggcggtcga tccggcctgg gaatcacgca gcgactggga aatctacaaa ggtattgcca
     3601 aagcattttc gcaagtgtgc gtgggccatc ttggcaaaga aaccgacgtg gtattacaac
     3661 cactgctgca tgactctccg gcagagctct cacagccgtg tgaagtgctc gactggcgca
     3721 aaggcgaatg cgatctgatc ccgggtaaaa ccgcgccgaa tattgtggcg gtggagcgcg
     3781 actaccctgc tacgtatgaa cgctttacct cgctcgggcc attgatggac aaacttggca
     3841 atggcggtaa agggatttcg tggaataccc aggatgaaat cgatttcctc ggcaaactca
     3901 attacaccaa gcgtgatggc ccggcacagg ggcgtccgct gattgacacc gccattgacg
     3961 cttcagaagt gattctggca ctggcaccgg aaaccaacgg tcatgttgca gtcaaagcgt
     4021 ggcaggcgct gggcgagatc accgggcgcg aacataccca tctggcgctg cacaaagagg
     4081 acgagaagat tcgctttcgc gatattcagg cgcagccgcg taaaattatc tccagcccca
     4141 catggtccgg tctggaaagc gatcacgtat cctataacgc gggatacacc aacgttcatg
     4201 agttaattcc gtggcgcacg ctgtcgggac gccagcagct ctatcaggat catccgtgga
     4261 tgcgtgcttt tggtgaaagc ctggtggctt atcgcccgcc tatcgacacc cgtagcgtca
     4321 gtgagatgcg ccagataccg ccaaacggct tccaggaaaa agcacttaac ttcctgacgc
     4381 cgcaccagaa atggggcatt cactcaacct acagtgaaaa cctgctaatg ctgacgctct
     4441 ctcgcggtgg accgattgtc tggatcagcg aaacagatgc ccgagaactg accattgtcg
     4501 ataacgactg ggtggaagta tttaacgcca acggcgcgct gactgcccgc gcggtggtca
     4561 gccaacgtgt accgccgggc atgaccatga tgtatcacgc ccaggaacgc attatgaata
     4621 ttcctggttc ggaagtaact ggcatgcgcg gcggcattca taactcggtc acccgcgttt
     4681 gcccgaaacc gacgcatatg attggcggtt acgcgcagct ggcctggggc tttaactact
     4741 acggcaccgt cggttcgaac cgcgacgaat tcatcatgat ccgcaagatg aagaacgtta
     4801 actggctgga tgatgaaggt cgcgatcagg tacaggaggc gaaaaaatga aaatacgttc
     4861 gcaagtcggc atggtgctta acctcgataa atgtatcggc tgccatacct gttcggtgac
     4921 ctgtaacaac gtctggaccg gacgcgaagg catggagtac gcatggttta acaacgtcga
     4981 aaccaaaccg ggcattggtt atccgaaaaa ctgggaagat caggaagagt ggcaaggcgg
     5041 ctgggtccgc gatgtgaatg gcaagatccg tccgcgtctg ggcagcaaga tgggcgtaat
     5101 aaccaaaatc ttcgccaacc cggtggtgcc gcagcagatt gatgattact acgaaccttt
     5161 caccttcgac tacgaacatt tgcatagcac accggaaggc aaacatatcc ctactgctcg
     5221 cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctggggcc caaactggga
     5281 agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca
     5341 aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga
     5401 acactgcctc aatcccagct gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga
     5461 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgca
//
LOCUS       DAJTYL010000180         5492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYL010000180 DAJTYL010000000
VERSION     DAJTYL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.181"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..451)
                     /locus_tag="OO572_003739"
     CDS             complement(<1..451)
                     /locus_tag="OO572_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6662571.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKA"
     gene            complement(448..1323)
                     /gene="nanK"
                     /locus_tag="OO572_003740"
     CDS             complement(448..1323)
                     /gene="nanK"
                     /locus_tag="OO572_003740"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6662572.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1320..2009)
                     /locus_tag="OO572_003741"
     CDS             complement(1320..2009)
                     /locus_tag="OO572_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6662573.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2057..3547)
                     /gene="nanT"
                     /locus_tag="OO572_003742"
     CDS             complement(2057..3547)
                     /gene="nanT"
                     /locus_tag="OO572_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6662574.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3657..4550)
                     /gene="nanA"
                     /locus_tag="OO572_003743"
     CDS             complement(3657..4550)
                     /gene="nanA"
                     /locus_tag="OO572_003743"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6662575.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4672..5463)
                     /gene="nanR"
                     /locus_tag="OO572_003744"
     CDS             complement(4672..5463)
                     /gene="nanR"
                     /locus_tag="OO572_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6662576.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFDEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1319 a         1552 c         1414 g         1207 t
ORIGIN      
        1 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
       61 gcttatgcgg ctctcctggc ataaacacgg cgaacattcc cggtttcaga atgatggttt
      121 gctcgttttc aatggcgcta caaagctggt aatcatcttc atggtggaac tcttcacatt
      181 ggcgcgcagt gcctgccatg ccaaacagaa tccgttcctc accgtttaat aacagctgga
      241 tatcaatgta ttgctcgtgc aattccgctt ttttctcgac gggcgattga gtgttaaacg
      301 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
      361 gcggtctggc agctaaggcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
      421 acggtaatga ctgtacttca cccatcatca taatttttct ccctgggcca acagcgcagc
      481 cccaagtaaa cctgcatcat ggcggtaatg cgccgccagt aaatcaacat gaaatgccgc
      541 tggctcctgc gccagataca tttccaccaa cgccagatac ccttctgcca gaccaacgct
      601 gccaccaacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
      661 aagcgtacgt gcggagcgat gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg
      721 tgtgaaaata gttctcgcat ccgcgccagc cagctcgccc tgcgctgccg ccgcaatgcc
      781 gcgaccagaa gcaattgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
      841 tggatcggca agcgtatgcc cgatgtgccc cgccagaccg ccagggccgg taagcagttt
      901 gccgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaga ccatatcggt
      961 tatatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
     1021 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
     1081 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
     1141 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
     1201 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
     1261 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtatggt
     1321 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
     1381 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
     1441 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
     1501 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
     1561 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
     1621 atcgccagta aaccgtgatg atggatacgt gccagcagcg tttcaacagg caccggacgc
     1681 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
     1741 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
     1801 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
     1861 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
     1921 tcgagcgggc tgtcaggaac cggctggcag gagacaatca ggccaccgtt agcagcgatt
     1981 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
     2041 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
     2101 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
     2161 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
     2221 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
     2281 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
     2341 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
     2401 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
     2461 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
     2521 ctttgcgggt tcccagccag tcaccgagga agccacctac gcagcatccc acggccgcgc
     2581 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
     2641 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
     2701 ccaccatcag cattacgccc gttggccagc gtttacccgt actttgcacc ataaagctga
     2761 taaagattgc ggcgcataac agcccgagaa cagcgacgat tgcagcattt tgcaggttac
     2821 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
     2881 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
     2941 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
     3001 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
     3061 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
     3121 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
     3181 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
     3241 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
     3301 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
     3361 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
     3421 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
     3481 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
     3541 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
     3601 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
     3661 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
     3721 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
     3781 gttttcaggc cgcggaatac gcccgttttg atcagtaaat caatgacttt gttgcactca
     3841 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
     3901 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
     3961 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
     4021 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
     4081 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
     4141 tacaccacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
     4201 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
     4261 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
     4321 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
     4381 gaaaggcttt gtacaaaggc ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc
     4441 tgctgaatat tgaactgaac caggcggcgc agactcgctt tatccagtgc ttgttgttga
     4501 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
     4561 gaagtgatgc ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
     4621 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
     4681 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
     4741 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
     4801 ctgttgataa ctaacgttgt tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc
     4861 ggcaataagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccggggat
     4921 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
     4981 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg
     5041 ttcagccgca tagcgcacca ggctggattc aaagaacaga cgtaattgtt cgaaatgggc
     5101 aatcccacca ggatgagaaa ggaaatcttt cgccatgccg gaaagctcgc cgatgatagt
     5161 gtccgcagaa ggtcgagaga cacgagcgcg ttcgccgttg tttatttgca ccagaccttt
     5221 gcgttttaac gctgccagcg cttcacgcac agaaggacgc ccgacgttaa agaacgccat
     5281 cagttcgcgt tcagacggta attgttcacc ttcgtcaaat tcacgacggc ggatcatctg
     5341 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
     5401 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
     5461 cattctgtaa ggtcagtgtg attaacatca tc
//
LOCUS       DAJTYL010000181         5427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYL010000181 DAJTYL010000000
VERSION     DAJTYL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.182"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            10..1362
                     /gene="celF"
                     /locus_tag="OO572_003745"
                     /gene_synonym="chbF"
     CDS             10..1362
                     /gene="celF"
                     /locus_tag="OO572_003745"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6662577.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1375..2133
                     /gene="chbG"
                     /locus_tag="OO572_003746"
     CDS             1375..2133
                     /gene="chbG"
                     /locus_tag="OO572_003746"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6662578.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2180..4441)
                     /gene="katE"
                     /locus_tag="OO572_003747"
     CDS             complement(2180..4441)
                     /gene="katE"
                     /locus_tag="OO572_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6662579.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4624..4887
                     /gene="cedA"
                     /locus_tag="OO572_003748"
     CDS             4624..4887
                     /gene="cedA"
                     /locus_tag="OO572_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6662580.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5164..>5427
                     /locus_tag="OO572_003749"
     CDS             5164..>5427
                     /locus_tag="OO572_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662581.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQD"
BASE COUNT         1345 a         1202 c         1413 g         1467 t
ORIGIN      
        1 ggagaaatta tgagccagaa attaaaagtc gtcactattg gtggcgggag cagctatacc
       61 ccggagttac tggaaggatt tattaagcgt tatcacgaat tgccggtcag cgaattatgg
      121 ctggtggatg tcgagggtgg caaagctaaa ctggatatta ttttcgatct ctgccaacgg
      181 atgattgata acgctggcgt cccgatgaag ctttataaaa cgctggattg ccgcgaagca
      241 ttgaaagatg ctgatttcgt tactacccaa ctgcgcgttg gccaattacc ggcgcgtgaa
      301 ctggatgaac gtattccatt aagtcatggt tatcttggtc aggaaaccaa cggcgcgggc
      361 ggtttgttta aaggtctgcg taccattccg gtgatttttg acatcgtaaa agatgtcgaa
      421 gaactttgtc cgaatgcatg ggtgattaac ttcactaacc cggcgggaat ggttactgaa
      481 gccgtttatc gtcataccgg atttaaacgc tttatcggcg tgtgtaatat tccgatcggc
      541 atgaagatgt ttattcacga tgttctgatg ctgaaagaca gcgatgattt atctatcgat
      601 ttgtttggcc tcaaccatat ggtgttcatt aaggatgtgc tggtaaatgg caagtcgcgc
      661 tttgccgaat tgcttgatgg tgtggcatcc gggcagttaa aagtatctgg cgttaaaaat
      721 attttcgatc tgccatttag cgaaggctta attcgttctc tgaatctgtt gccgtgttct
      781 tatttgcttt attacttcaa gcaaaaagag atgctggcta ttgaaatggg cgaatactac
      841 aaaggcggcg cacgagcgca ggtagtacag aaagtcgaga aacaactttt tgagctgtat
      901 aaaaacccgg agttgaaagt taagccgaaa gaactggaac agcgcggtgg ggcttattac
      961 tctgatgcag cgtgcgaagt gatcaacgct atctacaacg acaagcaagc tgaacattac
     1021 gttaatatcc cgcatcatgg gcatattgat aatattccgg cagactgggc ggtagaaatg
     1081 acctgtaagc tggggcgcga tggcgcgacg ccacatccgc gcattacgca tttcgatgat
     1141 aaagtgatgg ggctgattca caccattaaa ggcttcgaga ttgctgccag caacgccgca
     1201 cttagcggag aatttaacga tgtgttactg gcgctaaacc ttagtccgtt ggtgcattcc
     1261 gatcgcgatg ctgagctgct ggcacgcgag atgattctgg cgcacgagaa atggctgcca
     1321 aattttgccg actgcatcgc agagcttaaa aaagcacatt aaccgaggct gattatggaa
     1381 cgcttactga ttgttaatgc cgatgatttt ggcttaagca aaggtcagaa ctacggcatt
     1441 atcgaggcct gtcgcaatgg gattgtcaca tcgacgacgg cgctggtgaa tgggcaggct
     1501 attgaccatg cggtgcagtt gagtcgtgat gaaccgagtc tggccatagg gatgcacttt
     1561 gtccttacta tgggcaagcc actgacagct atgccggggt taacccgcga tggtgtgctg
     1621 ggaaaatgga tctggcagtt ggcagaagaa gatgctttac cgctggaaga aattactcag
     1681 gagcttgcca gtcagtattt gcgtttcatt gagctatttg gacgcaaacc tacgcatctt
     1741 gatagccatc atcatgtgca tatgttcccg cagattttcc cgattgttgc caggtttgcg
     1801 gctgaagagg ggattgcgtt gcgcatcgac cgtcagccgc tatctaacgc tggtgattta
     1861 ccggctaatc tgcgcagttc gcagggattc agtagcgcgt tttatggtga agagattagc
     1921 gaggcactgt tcctgcaagt gctggatgac gcaagccatc ggggtgatcc ttcgctggag
     1981 gtgatgtgtc atccggcgtt tatcgataat actatccgtc agagtgctta ctgtttccca
     2041 cggttaacgg agctggaagt gctgacatca gcgtcgttga aatatgcaat tgctgagcgt
     2101 ggttatcggt tgggtagtta tcttgatgtg taagtaaggt ggtaaggtca ctccccaggg
     2161 gacgtgacct tatccatatt tacgccggaa ttttgtcaat cttaggaatg cgtgaccaca
     2221 cgcggtgttc tgccatcagc gttaacagtt catccataaa actaccgtcg gtgctgtcag
     2281 cttccacaat cccttcttca ccctggtcag ctaccttgat tgttgcttta aacttgcgcg
     2341 cgtctcccgc cagcgcaatc ggtttaaggt gtttgtaggc ttccatcaga tagtagttgg
     2401 catcgccgtt gtcagcgata tccgcgatat tgccgcaagg gacaatgacc gcatcgaccg
     2461 tcagcgaagg tgcaccggca aaggtagcgg ctataggcaa caccgtaccg tcatccgcag
     2521 tcacttcacc cattcgggag tagagcagtt tggcatgaac gcctttggcc ttcatggcct
     2581 tgagaatggc cagaaggtct gccgatctca cttcatcatt aagtaaaatc gctaccacgc
     2641 gacctttcac atcaccgtca ggaatggcgt acaaacttaa ggatggatcc tttttcagac
     2701 cgttgacgtc cggaggtggg gtgatattca gctggtcgtc agtcagttcg ataccgagat
     2761 ttttcgccac cgcctgggcc agagtgagat caatatgcgc cagctggtca acaacgcgct
     2821 cacgaatata cggacgaacg actttgctta actcaaaact gaaaccatcg acaatatggc
     2881 gctgctcgaa tggcgtctga cttagccaga acagacgcgg atgggaataa tattcgccaa
     2941 acgatgggct gcgctcgcga actttattgc cttccacgcg ctcttggtat gattcaaaac
     3001 cgccgcgttt cggccccggt ggtgtttcgc gcggccagtt atcgttgatc gagttcggtt
     3061 cataattcgc cgggttagtg tcgatcccca tacgatgcat gccgtcacgc tggaaattat
     3121 ggtaagggca ggtcgggcgg ttaatcggaa tctcatggaa attcggccca ccaagacgac
     3181 tgatttgtgt atcggtatag gagaacaaac gtccctgcaa caggggatcg ttggtgaagt
     3241 ccagaccagg aacaatatga cctggatgga atgctgcttg ttcgttttcg gcaaagaagt
     3301 tatccggatt gcgattgagc accattttgc cgacacgctg aacgggcacc agttcttccg
     3361 ggatcagttt ggttggatcg agaagatcga agtcgaactt gaattcatct tcttcaggaa
     3421 tcaactggaa gcccagttcg tattccggaa aatcgcctgc ttcaatggct tcccacaact
     3481 cgcggcggtg gaagtccggg tcacgtccgg tgagtttttg tgcttcatcc caaacgagtg
     3541 aggctttacc tgccagtggt ttccagtgga aacgtacaaa cgttgccttc ccttcggcat
     3601 taatcaggcg gaaggtgtga ataccgaagc cttccatggt gcggtaactg cgggggatgc
     3661 cgcgatccga catcgcccac atcacgttgt gcagagtttc aggttgcaga gaaacataat
     3721 cccagaaagt atcgtgggca ctttgccctt gtggaattgc ccagtgcggt tctggtttta
     3781 ccgcatgaac aaaatcgggg aatttatgcg catcctggat aaagaagatt ggcgtgttat
     3841 tgccaacgag gtcaaaaata ccctcttcag tatagaactt ggtggcaaaa ccacggatat
     3901 cacgcacggt atcggcagag ccagcgccac cctgaacggt agagaaccta acaaatactg
     3961 gggtgatttt gttcggatct gagaggaaat ccgctttggt gatatcgctt aagcttttat
     4021 atggctggaa ataaccgtga gctgctgagc cgcgtgcatg aacaatacgt tccgggatgc
     4081 gctcatggtc aaagtgggtg attttctcgc gcagaataaa atcttccagc agcgttggac
     4141 cacggttacc ggcacgcagt gagttttgat catcggcgat gcgcacgccc tgattagtgg
     4201 tcagcgcata attttcactg cctttgcgta cgtcttccag agaattaagt ttttcgttac
     4261 gtgtatcagg ggctttcagg ctcccagggg cggtaggttg tgcacctggc ggtgttggtt
     4321 cagccgctgg acgatgagat ccgtcctcag gtgccagtga gtccatcccc ggtttcgctt
     4381 cgctggaatc gtgtagtggt gactggtgct gatgtgggtt cttttcgtta tgttgcgaca
     4441 ttgaactcgt ctccttaatt tattactgaa agggccgctg ttttggtcaa aaaactctct
     4501 aactatagac caccagccag atcccgcttc ggagactatt ctggaaacgg tgttatgaaa
     4561 tacacgcaaa gcgggacgta caggggctaa atcggctaaa ctacagtttt tctaattttt
     4621 aatatgcgtt tagtgaagcc agtaatgaag aaaccgctcc gccagcaaaa ccgccagatt
     4681 attagctatg tcccacgcac ggaacccgcg ccgccagaac atgcgataaa gatggattcg
     4741 tttcgtgatg tctggatgct gcgtggcaaa tatgttgcgt ttgtactgat gggagagtca
     4801 tttctgcgct caccggcgtt tactgtgcct gaatctgccc aacgttgggc aaatcagatc
     4861 cgccaggaag gggaagtgac tgagtaagga ttacatacct cccggttttg ccgggagtta
     4921 caggacctga aattctgcat ttcgttgcta cattcattca aaccacctgt aacaaaaagc
     4981 tgcctttgac gagctattgc tataaataag agatcttttt tctttaatta tttatttatt
     5041 ttaaaatggt taagttttgc taatcatgat gagttatttt tgatagttca tcctgtggcg
     5101 tcgctcttaa acttatattg taaatgactt agatttaaat aagataataa ttttgttaac
     5161 aatatgaggc gggagatgtt ttctacaaac ttaatacaga gtaattatgg tgatttaaat
     5221 attaaaagcc tggctttcga ctcttttaaa gaaaggcttg agtctaccat ggcggcactc
     5281 actttcttta tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc gaacttcaat
     5341 cgtatgattg cctatatgtc taacattaac tatgatgcct caaagctagg agcgccagca
     5401 cttagttttg atacgtatct gcaggac
//
LOCUS       DAJTYL010000182         5262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYL010000182 DAJTYL010000000
VERSION     DAJTYL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.183"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..131
                     /gene="ssuC"
                     /locus_tag="OO572_003750"
     CDS             <1..131
                     /gene="ssuC"
                     /locus_tag="OO572_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="part of the ABC type transport system for
                     alkanesulfonate SsuABC; SsuB the ATP-binding subunit and
                     SsuC the permease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6662582.1"
                     /translation="DVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            128..895
                     /gene="ssuB"
                     /locus_tag="OO572_003751"
     CDS             128..895
                     /gene="ssuB"
                     /locus_tag="OO572_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6662583.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(1102..3717)
                     /gene="pepN"
                     /locus_tag="OO572_003752"
     CDS             complement(1102..3717)
                     /gene="pepN"
                     /locus_tag="OO572_003752"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6662584.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            3983..5185
                     /gene="pncB"
                     /locus_tag="OO572_003753"
     CDS             3983..5185
                     /gene="pncB"
                     /locus_tag="OO572_003753"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6662585.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
BASE COUNT         1203 a         1352 c         1408 g         1299 t
ORIGIN      
        1 cagacgtggt ggtagtcgct attattcttt acgccctgct cggcaaactt gctgacgtca
       61 gcgcgcagct gttagagcgc ctctggttgc gctggaaccc ggcttatcat ttgaaggagg
      121 ccacgatatg aatactgctc gtctgaacca gggcacacca ttgttgctca atgcagtaag
      181 caaacattac gcggaaaata tcgtcctgaa ccaactggat ttacatattc cggcaggtca
      241 gtttgtggcg gtggtgggcc gcagcggtgg tggtaaaagt accctgctgc acctgctggc
      301 cggtctggaa acgccaaccg caggcgatgt gttagcgggc accacaccgc tggctgaaat
      361 tcaggatgat acgcgaatga tgtttcagga tgcgcgtctg ctgccgtgga aatcggtgat
      421 tgataacgtt gggttaggcc ttaaaggtca gtggcgcgat gccgcgcgtc gggcgttggc
      481 tgcggcagga ctggagaatc gcgccgggga atggcctgcc gcactttctg gcgggcagaa
      541 acagcgtgtg gcgctggcaa gagcgttgat tcatcgaccg ggtttattgt tgcttgatga
      601 accgctcggg gcactggacg ccttaacgcg actcgagatg caggatttga ttgtgtcact
      661 ttggcagcag cacggcttta ccgtactgtt ggtgacgcat gatgtgagcg aagcggtagc
      721 aatggctgac cgggtgttgt taattgaaga gggaaaaatt agtctggatt tgacagtgga
      781 tatcccccgt ccgcgtcgat tagggtcggt gcgattagcg gagctggaag cggaagtatt
      841 gcaacgggta atgcggcgtg ggcattctga acaactgatt cggcgtcatg gttaatatgc
      901 cggatgtggc gcacaaaacc agcattttca acggacttgt aggcctgata agacacgtca
      961 agcatcgcat caggcagttg ggcacggctg ccggatgcgg cgtgaacgcc ttatccagcc
     1021 tacaaaacca gcaatttcaa cggagttgta ggcctgataa gacgcgtcaa gcgtcgcatc
     1081 aggcattttg gctcaaaagt atcaagccag tgctttagtt atcttctcgt aaagatcgcc
     1141 agagagattt tccagccctt tcaactgttc cagcgccgcg cgcattttct cctgacgttt
     1201 ggcatcataa cgtttcaggc gaatcagcgg ttcaatcaga cgtgaagcta cctgcgggtt
     1261 acggctgttg aggtcggtaa gcatttccac caggaactgg taaccgctgc catcttcggc
     1321 atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc aacgaacgaa tacggttcgg
     1381 gttgctcatg gtaaatgagc gatgctgcaa cagaccacgc accgtctcca gcacattcgc
     1441 cgccgggctg gtggcttgca ggataaacca tttatccatc accagaccgt cctgatgcca
     1501 cttatcgtcg tactcctgca tcagcgcgtc acggcaaggc agctgtgcag caaccgccgc
     1561 agaaagcgct gccagcgcat cggtcatatt gtttgcttca tggtattgct tgctcaccag
     1621 cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc aggcaggcat tacgcagagt
     1681 gcgttttgca atatcttcat gctcaacacg gtactcgctc tggtaattcg cgttgtaaat
     1741 agccagcagc tcatccgcca gttcagtcgc cagagtacga gtgagtgctt cgcgtacttc
     1801 ggcaatagca atcgggtcta tgatatcgaa cagttcagcc atttcattga cagaaggcag
     1861 cgtcaggatt tctgccgcca gcgccggatc aatcttctca tcgagcagca ccgcgcggaa
     1921 agcgtcagcc acatgtaccg gcagagacag cggctgccct tgctgatgac gggcgacgtt
     1981 cagcttgatg taggttgcca gcaaactttg cgccgcatcc caacgggaga aaccattacg
     2041 cgcatgacgc atcaggaagg tcagttgctg atcgctccac ttatattcca gtttcactgg
     2101 cgcagagaat tcgcacagca gcgcaggcac cggctggaag tagacattat caaaaacaaa
     2161 ggtctgttcc gcctgggtga cgttcagcac ggaattcacc ggatgaccgc ctttctgcaa
     2221 cgggatcact ttaccttcgt tgtcatacaa ttcgatggca aacggaatat gcagcggctg
     2281 tttttctgcc tgatccggcg tggctggcgt gcgctggctg atggtcaggg tgtactgctc
     2341 agtttccgga ttgtagtcgt ctttgacggt cacaaccggc gtaccggact ggctgtacca
     2401 acggcggaaa tgggagagat caacattcga cgcatcttcc atcgcctgca caaagtcgtc
     2461 acaggtcgct gcgctaccat catgacgctc gaaatagagt tgcatccctt tctggaagtt
     2521 ttcttcgccc agcagggtgt ggatcatacg gatcacttcc gcgcccttct cgtaaacggt
     2581 cagggtgtag aagttgttca tctcaatgac catgtccggg cggattgggt gcgccatcgg
     2641 gctggcgtct tccgcaaact gcaatccgcg catggtgcgc acattattga tgcggttaac
     2701 tgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg aagacggtta aaccttcttt
     2761 caggctgagc tggaaccagt cgcggcaggt cactcggtta ccggtccagt tatggaaata
     2821 ttcatggccg ataacgcgtt caatatcgag gtaatctttg tcggtggcgg tgtcggtgcg
     2881 ggccagcaca tatttggagt taaagatatt cagaccctta ttctccattg cgcccatatt
     2941 gaagaaatcc accgcgacga tcatatagat gtcgaggtca tactccaggc caaagcgttc
     3001 ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc cacggcgcgc gatcaaggtt
     3061 gccgcgatcg acgtacagct ccagtgctac ttcgcgacca gaacgcgtgg taaaggtatc
     3121 gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg tagcacggtt tcgggaacgg
     3181 gtcctgccac tgtacccaat ggcgtccgtt ttcgagttcg ccttgcgcaa cgcggttacc
     3241 attggaaagc aggaagggat atttgatttt atcggcaata attttggtgg taaaacgcgc
     3301 cagcacgtcc gggcggtcga gataatacgt aatatggcgg aaaccttcgg cttcacactg
     3361 ggtgcaaagc gcatcgcctg actgataaag cccttccagc gccgtattcg ccgccaggct
     3421 tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa ttactgatga ccagtgcgcc
     3481 ctcttcttct tctttccagg cggtccacgg ctcatcatta atatgaacag aaaccagttt
     3541 gaggtcttcg ccgttgagac gaagcggagc atctgatgca ccatgacgga cagcctggct
     3601 gaccgcggta acgaccgtct tttgcgcgtc gaggtcaaag gtcaagtcaa tatcagtaat
     3661 ctggtaatcc ggcgcacgat aatcgtgacg gtatttggct tgtggctgtt gagtcataaa
     3721 taacctttag catcttttat agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg
     3781 ctatgcagaa tcttcatctt ttcaggtaca aacgccttta ttgctacatt tttataacat
     3841 acaccgcgta atgccatcga ccagaaaggt ggcatatggt gtgatcgggg ttcaataaat
     3901 tgcgaaacaa ggtatactcc agcagttcct gaagatgttt attgtactaa acgctcctgt
     3961 acgaggacgc tactgcgcac ctatgacaca attcgcttct cctgttctgc actcgttgct
     4021 ggatacagat gcttataagt tgcatatgca gcaagccgtg tttcatcact attacgatgt
     4081 gcatgtcgcg gcggagtttc gttgccgagg tgacgatctg ctgggtattt atgccgatgc
     4141 tattcgtgaa cagattcagg cgatgcagca cctgcgcctg caggatgatg aatatcagtg
     4201 gctctctgcc ctgcctttct ttaaggccga ctatcttaac tggttacgcg agttccgctt
     4261 taacccggaa caagtcaccg tgtccaacga taatggcaag ctggatattc gcttaagcgg
     4321 tccgtggcgt gaagtcatcc tctgggaagt tcctttgctg gcggttatca gtgaaatggt
     4381 acatcgctat cgctcaccgc aggccgacgt tgcgcaagcc ctcgacacgc tggaaagcaa
     4441 attagtcgac ttctcggcgt taaccgccgg tcttgatatg tcgcgcttcc atctgatgga
     4501 ttttggcacc cgtcgccgtt tttctcgcga agtacaagaa accatcgtta agcgtctgca
     4561 acaggaatcc tggttcgtgg gcaccagcaa ctacgatctg gcgcgtcggc tttccctcac
     4621 gccgatggga acacaggcac acgaatggtt ccaggcacat cagcaaatca gcccggatct
     4681 agccaacagc cagcgagctg cacttgctgc ctggctggaa gagtatcccg accaacttgg
     4741 cattgcatta accgactgca tcactatgga tgctttcctg cgtgatttcg gtgtcgagtt
     4801 cgctagtcgg tatcagggcc tgcgtcatga ctctggcgac ccggttgaat ggggtgaaaa
     4861 agccattgca cattatgaaa agctgggaat tgatccacag agtaaaacgc tggttttctc
     4921 tgacaatctg gatttacgca aagcggttga gctataccgc cacttctctt cccgcgtgca
     4981 attaagtttt ggtattggga ctcgcctgac ctgcgatatc ccccaggtaa aacccctgaa
     5041 tattgtgatt aagttggtag agtgtaacgg taaaccggtg gcgaaacttt ctgacagccc
     5101 tggcaaaact atctgccatg ataaagcgtt tgttcgggcg ctgcgcaaag cgttcgacct
     5161 tccgcatatt aaaaaagcca gttaatatca tcagggagct aatcggctcc cttttttacc
     5221 tttaattccg aaatctttcg ctgcatttgc gaattctgct tg
//
LOCUS       DAJTYL010000183         5242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYL010000183 DAJTYL010000000
VERSION     DAJTYL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.184"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..160)
                     /locus_tag="OO572_003754"
     CDS             complement(<1..160)
                     /locus_tag="OO572_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662586.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIV"
     gene            236..2797
                     /gene="mutS"
                     /locus_tag="OO572_003755"
     CDS             236..2797
                     /gene="mutS"
                     /locus_tag="OO572_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6662587.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2903..3555
                     /gene="pphB"
                     /locus_tag="OO572_003756"
                     /pseudo
     CDS             2903..3555
                     /gene="pphB"
                     /locus_tag="OO572_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3606..4373)
                     /locus_tag="OO572_003757"
     CDS             complement(3606..4373)
                     /locus_tag="OO572_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6662588.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4569..5242
                     /locus_tag="OO572_003758"
                     /pseudo
     CDS             4569..5242
                     /locus_tag="OO572_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1296 a         1353 c         1359 g         1234 t
ORIGIN      
        1 gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt acttgcttca
       61 taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc aagaactcgc
      121 cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca
      181 acgaaaataa aaaccatcac accccattta atatcaggga accggacata accccatgag
      241 tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca agctgaaagc
      301 ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga
      361 cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc
      421 gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact atctcgccaa
      481 actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc cggcgaccag
      541 caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca tcagcgatga
      601 agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg acagcaaagg
      661 tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg aaccggccga
      721 ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc tgtatgcaga
      781 agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg
      841 ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca cccgcgatct
      901 ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt gtctgttgca
      961 gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca ccatggaacg
     1021 tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa ttactcagaa
     1081 cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg tcacgccgat
     1141 gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct
     1201 tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc agccggtact
     1261 gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa ctgctcgccc
     1321 acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc gtgcgcagtt
     1381 agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg agtttgccga
     1441 gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg
     1501 tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg
     1561 cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac
     1621 gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc gtgggcaaag
     1681 ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg agcgctacat
     1741 cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca aagcactggc
     1801 actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt
     1861 gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg cggaacgggc
     1921 ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc gcattaccga
     1981 aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta acccgctgaa
     2041 tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg gtaaaagtac
     2101 ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct atgtaccggc
     2161 acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg cggcagatga
     2221 cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca atattttaca
     2281 taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa cgtccaccta
     2341 cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga ttaaggcatt
     2401 gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa tggaaggcgt
     2461 cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta tgcacagcgt
     2521 gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg cgggcgtacc
     2581 gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa
     2641 cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc cagaagaaac
     2701 ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc cgcgtcaggc
     2761 gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga tagtcttttg
     2821 ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta taataggaaa
     2881 gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca atgcccatca
     2941 ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat tacaatcccg
     3001 cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg gcgataatat
     3061 tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct ggtttatctc
     3121 cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg atggcaatat
     3181 gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca agaggcaata
     3241 gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa cgacataata
     3301 aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg caaagaaata
     3361 gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa tggcgagtta
     3421 caacaaataa acggcgctga ttattttata tttggacata tgatgtttga taacattcag
     3481 acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg gctgtcattt
     3541 tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga tgttatttca
     3601 ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg
     3661 gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa tggtaacgcc
     3721 agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg
     3781 gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc
     3841 acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca cagcatggtc
     3901 gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata
     3961 attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca
     4021 gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc
     4081 agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat agccgctttt
     4141 tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga
     4201 gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc
     4261 cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat actgacaatg
     4321 cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc
     4381 tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac
     4441 taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca tctcaccata
     4501 tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta aattaacaag
     4561 gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg
     4621 gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc gcagacctga
     4681 acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg
     4741 cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg gcctaggtta
     4801 aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa
     4861 tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat
     4921 tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg
     4981 aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta cccgtgctgg
     5041 aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa
     5101 aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac
     5161 ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg
     5221 gaaattcctg gatgttcgaa aa
//
LOCUS       DAJTYL010000184         5172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYL010000184 DAJTYL010000000
VERSION     DAJTYL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.185"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..4042
                     /gene="sepA"
                     /locus_tag="OO572_003759"
     CDS             <1..4042
                     /gene="sepA"
                     /locus_tag="OO572_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6662589.1"
                     /translation="VSELARRVTCKSHRRLSRRVILTSVAALSLSSAWPALSATVSAE
                     IPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGD
                     HTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPA
                     EVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNS
                     YNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYG
                     NNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAANGTGTLTQGNTTWDMHGKK
                     GNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQTWMGGGIITDKG
                     THVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQQADADGKVQAFS
                     SVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQAADYGAIITNNS
                     EKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYDSSTKQYFILKASSYSPFF
                     SDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQLNGNMDVNIPQLSGKDVL
                     ALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQFTLEKLKLDAATF
                     HLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVSSVEGSATATTVGDQSDYS
                     GNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGSFVNSSLTLGKGAKLTA
                     QSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLEDNASFSVKNMGYL
                     SSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSITGAQSTVNMINAL
                     WYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNTTFLMHTNNSRADQLNVTD
                     KLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGFSNVTPVISTEKT
                     DDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVW
                     ARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGALLTYTDSNASSHAFSGKN
                     KSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLGTKDYSSHSWYAGAEVGYR
                     YHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIGRTGVDVGRAFSG
                     DDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTVGMNAEIKDNMRL
                     GLELEKSAFGKYNVDNAINANFRYVF"
     gene            complement(4211..4825)
                     /gene="istB"
                     /locus_tag="OO572_003760"
                     /pseudo
     CDS             complement(4211..4825)
                     /gene="istB"
                     /locus_tag="OO572_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(4857..>5172)
                     /locus_tag="OO572_003761"
     CDS             complement(4857..>5172)
                     /locus_tag="OO572_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6662590.1"
                     /translation="EAFSYVLNQWDALCYYSDDGLAEADNNTAERALRTVCLGKKNYM
                     FFGSDHGGDRGALLYGLIGSCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLT
                     DK"
BASE COUNT         1354 a         1238 c         1339 g         1241 t
ORIGIN      
        1 tgtctccgaa ctggcccgca gggttacctg caaaagccat cgcagacttt cacgtcgggt
       61 tattcttacg tctgttgcag ctttatcact ttcctcggca tggccagctc tgtcagcaac
      121 ggtcagtgca gagatccctt atcagatatt ccgcgacttt gccgagaata aaggtcagtt
      181 tacgccgggg accacgaata tttccattta cgacaagcag ggcaatctgg tcggtaaact
      241 tgataaagcc ccgatggctg atttcagtag tgccactatt actaccggta gcctgcctcc
      301 gggagaccat acactttact cccctcagta tgtggttacc gccaaacatg tcagcggttc
      361 tgacacgatg agcttcggat acgccaaaaa cacctatact gcggttggta caaataataa
      421 tagcggtctt gatattaaaa cccgccgcct gagtaaactg gtgacagagg tggcgccggc
      481 agaagtgtct gacatcgggg ctgtgagcgg tgcctatcag gccggcggac gctttactga
      541 attctatcgc cttggggggg ggatgcagta cgtcaaagac aaaaatggta atcgcaccca
      601 agtgtatacc aatggcggat ttcttgttgg gggaaccgtc agcgcgctga actcctataa
      661 caacggacag atgattacag ctcagaccgg tgacattttt aatcccgcga atggacctct
      721 ggctaattat ctgaatatgg gggacagcgg ctcccccctg tttgcttatg attccctgca
      781 aaaaaaatgg gtactgattg gcgtgctttc ctcaggaact aactatggaa ataactgggt
      841 cgtcaccact caggatttcc ttggtcagca accgcaaaat gattttgaca aaactatagc
      901 ctacacctct ggtgaaggag tactgcagtg gaaatatgat gcggctaatg gcaccggcac
      961 actgactcag ggaaacacga cttgggatat gcatggaaag aaaggaaatg atctgaacgc
     1021 aggaaaaaac ctcctgttca ccggcaataa tggcgaggtc gttctgcaga attccgttaa
     1081 tcagggagct gggtatctgc agtttgccgg cgattacagg gtgtccgccc tgaacggcca
     1141 gacatggatg gggggcggga ttatcaccga taaaggaacc cacgttctgt ggcaggtgaa
     1201 cggagttgcc ggtgataacc tgcataaaac tggcgaaggt accctgacag taaacgggac
     1261 cggtgtgaat gcaggcggac tcaaggtggg ggacggtacc gttattctca accagcaggc
     1321 ggatgctgac ggaaaggtac aggctttcag ctctgttggt attgccagcg gtcgtccgac
     1381 agttgtgctt tctgactcac agcaagttaa tccggataat atttcgtggg gataccgtgg
     1441 aggacgtctg gaactgaatg gtaataatct gacctttacc cgtcttcagg cggctgacta
     1501 cggggccatc attaccaata acagtgagaa aaaatcaacg gtaacactcg accttcagac
     1561 attaaaagcc agtgacatca atgtaccggt taatactgtc agtatttttg gggggagagg
     1621 agcgccggga gacctatatt atgacagttc taccaagcaa tatttcatcc tgaaagcgag
     1681 ttcatattcc ccgttcttct ccgatctgaa caacagcagt gtctggcaga atgtcggaaa
     1741 agatcgtaat aaagccattg atactgtgaa gcaacaaaaa attgaggcca gcagccagcc
     1801 ttatatgtat cacggccaac taaacggcaa tatggatgtg aatattccgc agctctcagg
     1861 taaggatgta ctggctcttg atggttcagt taacctgcca gaaggttcga taaccaagaa
     1921 gtccggcacg ctgatattcc agggtcatcc agttattcat gctggaacga cgacttcttc
     1981 cagccagagc gactgggaaa cccgtcagtt tacgctggaa aaactgaaac tcgatgcggc
     2041 aacattccat ctgtccagaa acggcaagat gcagggagat attaacgcca ccaatggaag
     2101 tacagtcatt ctgggaagta gccgtgtctt tactgacagg agtgacggaa ccggtaatgc
     2161 ggtctcctct gttgaaggga gtgccactgc aaccacagtt ggtgaccaga gtgattacag
     2221 cggaaatgtt acgctggaaa ataaatcatc cctgcaaatc atggagagat tcaccggtgg
     2281 tattgaggct tatgatagca ccgtgagtgt gacctctcag aatgctgttt ttgaccgtgt
     2341 tggcagcttt gtcaacagca gcctgaccct cggaaaagga gcaaaactta cggctcagag
     2401 cggtattttc agcaccgggg ctgtggatgt aaaagaaaat gcttctctga ccctgacggg
     2461 gatgccttct gcgcagaaac aggggtatta ctcacccgta atttctacaa cagaagggat
     2521 taacctcgaa gataacgcca gcttttctgt taaaaatatg ggctatctga gttcggatat
     2581 tcatgcagga accactgcgg caaccattaa tctgggagac agtgatgctg atgcagggaa
     2641 gacggactct ccgttattca gctccttaat gaagggatat aacgctgttc tgagaggcag
     2701 tattacgggg gcacagagta cggtaaatat gatcaatgct ctgtggtact ctgacggaaa
     2761 atcagaggcc ggtgcactga aggctaaggg ctcgcgaatt gaactggggg acgggaaaca
     2821 ctttgccact ttacaagtaa aagagctttc tgcagataat accacgttcc tgatgcatac
     2881 caacaacagc cgggctgacc agttgaatgt cacagacaaa ctgtcaggca gtaataatag
     2941 cgtcctggtt gactttttaa acaaaccagc cagtgaaatg agcgtgacgt taattaccgc
     3001 accgaaaggg agtgatgaga aaacgttcac tgccggtacg cagcagattg gtttcagtaa
     3061 cgttacgcca gtaatcagca cggaaaaaac ggatgatgcc acaaaatggg tgctgacagg
     3121 atatcagact accgctgatg ccggagcctc gaaagccgca aaagacttta tggcatcagg
     3181 ttataagtcc ttccttacag aggtcaataa cctgaacaaa cgtatgggtg acctgcggga
     3241 tactcagggg gatgccggtg tctgggcacg cataatgaat ggtaccggtt cggcagatgg
     3301 tgactacagc gataactaca ctcacgttca gattggtgtc gacagaaagc atgagctgga
     3361 cggtgtggat ttatttacgg gggcattgct gacctatacg gacagcaatg caagcagcca
     3421 cgcattcagt ggaaaaaaca aatccgtggg tggcggtctg tatgcctctg cactctttaa
     3481 ttccggagct tattttgacc tgattggtaa atatctccat catgataatc agcacacggc
     3541 gaattttgcc tcactgggaa caaaagacta cagctctcat tcctggtatg ccggtgctga
     3601 agttggttat cgttaccacc tgacgaaaga gtcctgggtg gagccacaga tagagctggt
     3661 ttacggttct gtatcaggaa aagcttttag ctgggaagac cggggaatgg ctctgagcat
     3721 gaaagacaag gattataacc cactgattgg ccgtactggt gttgacgtgg gaagagcctt
     3781 ctccggagac gactggaaaa tcacagctcg agccgggctg ggttatcagt tcgacctgct
     3841 ggcgaacgga gaaacggttc tgcaggatgc ttccggagag aaacgtttcg aaggtgaaaa
     3901 agatagcagg atgctgatga cggtagggat gaatgcggaa attaaggata atatgcgttt
     3961 gggactggag ctggagaaat cagcgttcgg gaaatataat gtggataatg cgataaacgc
     4021 caacttccgt tatgttttct gacgaatggg ataggtctgc cattcggcag gcctttttct
     4081 gaaagaactc cgtgagcctg tttaagattc tgtgtaaatg ccttttctca gaagtgaccg
     4141 tccaggtatt agacgtcaac tagttttgcc gactggcgcc aactatactg gccgcccctt
     4201 cagtgcgaca tcaagtcacg cttgttactg ccttcgctgt ctttttcctg tagctttccc
     4261 ctttcagttc gaacatatat ccgtgatgga tcagtcggtc tgctgccgcc accgccatag
     4321 tctcatccac gaagatgctg ccccacatgc tgaacggatg gttactggtt atcaccaggc
     4381 tcccacgttc atagcgatgc gctattaact cgaacagcac acccgtttcg gcgctgtcgc
     4441 gtttgacata gccaagatca tccaccacta tcacccggta gcgatccagt ttcagtagca
     4501 gctcattcag tttcaactga gctctggctt tacgtagttc ctgcaacagc tctcctgcgc
     4561 tgtagaaccg ggcccggtag ccctggccta ctacgccatc cacgatcgct gccgccagat
     4621 ggcttttccc caacccgctg gccccgaaca gcagaacgtt ttctccagca tcaacccagt
     4681 ctgtcgtttc acagagttgc tggaactgag ctccattcag ttccggcaac tgaatgaagt
     4741 cgtattcgct tagcgttttg gcaactggca accgggcctc ctttttataa cgacgcagtt
     4801 tttcactctc gcgccacagg agttctgtaa gcgtcaacgg aaggccgtat tgacgcttat
     4861 ttatcggtga gaactacgtt ccatggcagc agttcgtcaa ctcggttgga aggccattcc
     4921 ggcagtacgc tcaggatatg gcgcagatac gcttctggat cgataccgtt caaccgacag
     4981 ctcccgatta gtccgtacag cagagctccg cgatcgcctc catgatcgct gccgaagaac
     5041 atgtaattct ttttcccgag acagacggta cgaagcgctc tttctgctgt gttattgtcc
     5101 gcctccgcca gaccgtcatc actgtaataa cagagggcat cccactgatt cagtacatag
     5161 ctgaacgctt cg
//
LOCUS       DAJTYL010000185         5139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYL010000185 DAJTYL010000000
VERSION     DAJTYL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.186"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO572_003762"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO572_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6662591.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="OO572_003763"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="OO572_003763"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6662592.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="pgeF"
                     /locus_tag="OO572_003764"
     CDS             1872..2603
                     /gene="pgeF"
                     /locus_tag="OO572_003764"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6662593.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..>5139
                     /gene="clpB"
                     /locus_tag="OO572_003765"
     CDS             2733..>5139
                     /gene="clpB"
                     /locus_tag="OO572_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6662594.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKL"
BASE COUNT         1258 a         1244 c         1401 g         1236 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcaacag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgc
//
LOCUS       DAJTYL010000186         4937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTYL010000186 DAJTYL010000000
VERSION     DAJTYL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.188"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            44..547
                     /gene="yeaK"
                     /locus_tag="OO572_003766"
     CDS             44..547
                     /gene="yeaK"
                     /locus_tag="OO572_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6662595.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(548..652)
                     /locus_tag="OO572_003767"
     CDS             complement(548..652)
                     /locus_tag="OO572_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662596.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            822..1268
                     /locus_tag="OO572_003768"
     CDS             822..1268
                     /locus_tag="OO572_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6662597.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1225..2043)
                     /gene="nimR"
                     /locus_tag="OO572_003769"
                     /pseudo
     CDS             complement(1225..2043)
                     /gene="nimR"
                     /locus_tag="OO572_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2140..3320
                     /gene="nimT"
                     /locus_tag="OO572_003770"
                     /pseudo
     CDS             2140..3320
                     /gene="nimT"
                     /locus_tag="OO572_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            3375..3722
                     /locus_tag="OO572_003771"
     CDS             3375..3722
                     /locus_tag="OO572_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6662598.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3744..3998)
                     /gene="yoaF"
                     /locus_tag="OO572_003772"
     CDS             complement(3744..3998)
                     /gene="yoaF"
                     /locus_tag="OO572_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6662599.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4181..>4937
                     /gene="dgcP"
                     /locus_tag="OO572_003773"
     CDS             4181..>4937
                     /gene="dgcP"
                     /locus_tag="OO572_003773"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6662600.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRL"
BASE COUNT         1149 a         1247 c         1226 g         1315 t
ORIGIN      
        1 ataaccttct gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa
       61 aggaagcgtg acgcatcagc agttaatcgc tttattatca caagaaggtg ctaacttccg
      121 cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggtaccgc
      181 tctaggccag ggtgctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca
      241 tgttctggcg atcctcgcag ccgatcaaca ggccgatctg agccaactcg ccagccatat
      301 aggcggatta cgcgcttcgt tggccagccc ggcggaagtt gatgaactca caggctgtgt
      361 cttcggcgcg atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct
      421 actcttcgaa cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat
      481 tttgaaaacc gcggattatt tgcgcattgc gcaaccagaa ctggtcaatt tccgccgcac
      541 tgcgtagcta gcctatccgt tcaataagca gaacggacaa aaccagcaca accgcaatag
      601 caaaaaacga tgatgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc
      661 cccgggagat agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag
      721 ttcgcctgat tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagca
      781 taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc
      841 tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt
      901 caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg
      961 aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg
     1021 ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg
     1081 tggcaattgc agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg acgttaatgg
     1141 gcagccagcc gcaactggtt gccgggctgc tggtgggcac tgttttaggc gtagcgctgt
     1201 ttcgcggcgt accggttggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga
     1261 aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaagcctt tcttaaacat
     1321 agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc
     1381 gcctttcact aatccctgaa gcgccataat cagctgtaac tgctgacgcc actggcgaaa
     1441 actcaacccc gtctctttca ctatcaggcg ggcaagattg cgttcactca tcgcaaaaaa
     1501 gccagcccat tgccccaatg ccccccactc gacgggccct ttcgccatca tctccaccat
     1561 cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg
     1621 gagttcatca aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt
     1681 cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca
     1741 acatggtcgg cattgtcact gcgctgggtt cgataaacag aaaacagagt tcagcgttag
     1801 ctgtcacttg attgctatgc ttcaccccgc ccggtatcca aaccgcatat tgtggcggca
     1861 ccatccacaa agcattttcc accgtacagg taattgcacc atgtaatgcc agtatcagct
     1921 gtcctttgcg gtgctgatgt acaggactgg ttaactcatc cgttccggcg tgaatacaaa
     1981 atgcgaccgc ggcttcatgg tgtcggtggg ttcatagcca ttgagattca acctgtgcat
     2041 cattttgtcc gaacttagcg ataatttgtc attttagctt gattcaacat aacaataaaa
     2101 acggtaaggt acagcctcgt ttgtaacaat gagaagcata tgacctgttc aacttcatta
     2161 agcggcaaaa acaggattgt ccttatcgct ggcattctga tgattgccac aacattacgc
     2221 gtcaccttta ccggcgcagc gccgttactg gatacgattc gttccgctta ctcgctgacg
     2281 acagcgcaaa ccggcttatt aaccaccctg ccattattgg cctttgcgct aatctcacct
     2341 ctggctgccc cggtggcgcg acgttttggt atggaacgta gcctgtttgc cgcgttactt
     2401 ttgatctgtg caggtatcgc aattcgttct ctcccttcgc cttacttatt atttggcggt
     2461 acagcggtca ttggcggtgg gattgcatta ggcaatgtct tactgccagg attaattaaa
     2521 cgcgatttcc ctcattccgt cgccagactt accggcgcat attccctgac aatgggagct
     2581 gcagcggcac tgggatcggc tatggtcgtg ccgctggctt tgaacggttt tggctggcaa
     2641 ggcgcgttgt tcatgctgat gtgttttcct ctgctggctc tttttttatg gctgccacag
     2701 tggcgaagtc agcaacatgc aaatttgagt acctcacgcg ccttacatac tcggggtatc
     2761 tggcgttcgc cacttgcctg gcaggtaaca ttgtttcttg ggatcaactc actggtctat
     2821 tacgtgatta ttggctggct tccggcgatc ctcatcagtc acggctatag cgaagcacag
     2881 gcgggttcac tgcatggttt gctgcaacta gccacagcag ctcccggttt gctgatcgca
     2941 cttttcttac atcatgtgaa agaccagcgt ggtattgcag cgttcgttgc cttgatgtgc
     3001 gcagtgggcg cggttgggct ctgctttatg ccagcgcacg cgatcacctg gactctgctt
     3061 ttcggttttg gttccggcgc aacaatgata ctggggttga cgttcattgg tcttcggcaa
     3121 gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg tatttgctgg
     3181 cagcctgtgg gccgccgcta atgggtaaaa tacacgatgc taacggtaac tggtccgtac
     3241 cactgatggg tgttgcaata ctttcactac tgatggcgat tttcggactt tgcgccggga
     3301 gagacaaaga aattcgctaa tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag
     3361 ggagaacaaa cacgatgaat attcagtgca aacgcgttta tgatccggct gaacagagcg
     3421 atggttatcg cgtactggtc gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag
     3481 cccttgatga gtgggataaa gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg
     3541 gcgaagtcgt cgattttgca acctttcgcg agcaatatcg cgcagaactg gcgcaacacg
     3601 agcaagaagg aaagcggctg gcggacatcg ccaaaaaaca gccgctgacc ctgctctacg
     3661 cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc cgactggcta cgtagcttgt
     3721 gattttagta cggcatccgg cggttatttt tcaccagccg gatggtcccg tcgccacaat
     3781 gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac cccttgcgct
     3841 gtctgcactg gaatgagcgt cccgcccttc tgctggcaat agaccgacgc cggatttgcc
     3901 ataccaatct gcggcggttt aggtgcttct ggctgagaag gggttgaaca accagccagg
     3961 accagcaagc aaggcagaac aaaactgata attttcattt attgatctca catatttatc
     4021 caagcttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt acattcactc
     4081 aaaacatatt acgtcttgtt tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt
     4141 aaataatcag cacttacccg ccgtatcctg gagttgttcc gtgtcagatc agattatcgc
     4201 ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa agtctggtcc gacagcttct
     4261 ggagatgctg gaaatggtca ctgatatgga atcaacctac ctgaccaaag tggatgtcga
     4321 agcgcgcctg cagcatatta tgtttgcccg taacagccag aaaatgcaca tcccggagaa
     4381 ttttaccgtc ttgtgggatt actcgttatg caaacgcgcc attgatgaaa actgcttttt
     4441 cagcgatgaa gtccctgacc gttggggcga ctgtattgcg gcacgcaatc ttggcatcac
     4501 cacatttctg agcacgccaa ttcacttacc ggatggatca ttctatggca cgctttgcgc
     4561 cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggttttac agttattcgc
     4621 cggactgatt gcacaatata ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa
     4681 tgctgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga atcggcgggc
     4741 gatttttgaa aatctgacga cgctgttttc cctcgcccgg catcttaacc ataagataat
     4801 gatcgcgttt atcgatctgg ataacttcaa attaatcaat gatcgttttg gtcataatag
     4861 tggcgatctg tttctcattc aggttggcga gcgccttaat acgctccagc aaaatggcga
     4921 agttattggt cgtctcg
//
LOCUS       DAJTYL010000187         4910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYL010000187 DAJTYL010000000
VERSION     DAJTYL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.189"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1063)
                     /locus_tag="OO572_003774"
     CDS             complement(<1..1063)
                     /locus_tag="OO572_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6662601.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLD"
     gene            complement(1079..1963)
                     /locus_tag="OO572_003775"
     CDS             complement(1079..1963)
                     /locus_tag="OO572_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6662602.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(1974..2579)
                     /locus_tag="OO572_003776"
     CDS             complement(1974..2579)
                     /locus_tag="OO572_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6662603.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2750..>4910)
                     /locus_tag="OO572_003777"
     CDS             complement(2750..>4910)
                     /locus_tag="OO572_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR6662604.1"
                     /translation="VEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLG
                     NLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTI
                     KGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLIN
                     DEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDI
                     ATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQP
                     VVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELY
                     KDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEM
                     KHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAY
                     FCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPK
                     ADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVL
                     KELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWW
                     STLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPL
                     LALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1452 a         1207 c         1001 g         1250 t
ORIGIN      
        1 aatccaggga actgcctgaa tcaaagccgg tactcattcc gagatgcagg cccgcagagt
       61 gcgcaccgaa caatttcatc gcctttcgca taaagcgata cgctaaatca accggcgata
      121 atggcacggg tccaccagaa aggagccgcc atcgatccgc tgatggtcct gtgcggtctt
      181 catgctgata atcaaatttt tgcgatttgt tagcatataa gcggcagata aagctttgca
      241 ttttttcaaa tgcctgcgcc ctgttctctt cacccaatgt gtcgtgataa aagcctggca
      301 gcagatgcag ctctttcaga gggctacgta atctctgata aaaatcaatc tgcggttggc
      361 gatgcaccac atagtcatca cctgatatca gaagttgcgt ggggagcgta atcgccgccg
      421 catcgctaac aatacgttca gacgttttat agagatcgag caagatattg acggcaatcg
      481 cccgtgtgat cagcggatca ttattgaaac tcgccacccg ttgccgatcg tgggtcaaat
      541 agcgcccttt cacataggaa ttaataaaaa atagaccacg cagacgatgc cataacgcca
      601 gcgcaggacg tgccagcggc acatacaatt taaccttaaa ggccggagaa gccagcacca
      661 gcccacgaat tgcaggtgca taatcatgaa cccatgtggc aacaagcact gcgccgacgc
      721 tttgcgcgat cactaccacc tcttccagtc cgacctggct gtcgctggca gcaaaacgga
      781 caaattcatc gacatcctgc actgagcgca taagagacgg gctataacca cgcggccccg
      841 aattttttcc atgcccccgg gcatcccagg cataaaaagc agtatcaggc atcgccagtt
      901 catcaacgag atgttgtaga cgaccagaat gttcatgccc gcgatgaaat aagacgatga
      961 cctttttcgc ccctggctgt aaagtgggcc agtgccgata atacaatgct gtattatcac
     1021 tggtggtaaa aaaatgttcc ccagggatgc gtgaattttc cattttctct attttccttc
     1081 agtaacagca gtagcgaata aaataaaaaa ataccggagc ggtgaaaatt aatgagtcaa
     1141 tcctgtcaag gagtccgcca tgtcctagta atagttttcc actatcttta acaccaatat
     1201 ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc actaatacca attaacaaac
     1261 ccgctaataa tgcctgtaat gtatttagcg gtgtcagcaa cggtccaata atcagtgacg
     1321 caatcatggt ggtgattacg ccccccagca gaccttccag cgtttttccc gggctgactt
     1381 tggggaccac ttttcttctg ccgcaggatt tcccccataa atactgtgca atatcgtttg
     1441 actcggttaa cgccaaaaga aataacacca gtaacgcccc gccctggata tttatggttg
     1501 gcaacattaa tagccaggcg gcatgactca aggcaaaaac ggtcattatc catccccaaa
     1561 aaatggcgct cacggtatgc tgaaaaccgg aggggtcacc cacaaaaact cgccaggtgg
     1621 ctaatatcag aaacccagtc aaaggtataa ataacagaaa cagctcaaag caattaaaac
     1681 cgataagcaa atagttaaga ggaataaccc aatataacca acgcgaaaaa tgtacggata
     1741 taagcgtaca atattctttc aatgccagaa aacttatcag cgtaaaaaac gtcaacgtca
     1801 tccagcgtgg gcctgaaatc accagtgaaa aacaaatgac gatgccccac caggtacgaa
     1861 tacgtaatgt aacttcatcc ccttttctct caggtaaccg ccatagcaga aaacggttta
     1921 tcagcttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca gcattatctt
     1981 tcagcgctca taagaagcac actccgacaa cgattaatcg cagtacagat aagcagaatt
     2041 gaagcaatgc tccataaaag attattccat tgcatccact gtggataaat ggcaacggcc
     2101 agaccccaca gaccaaatat taacgcgcga tcacttttgc caaatggacc ggcataactg
     2161 cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat ggtgcaaaat
     2221 aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaacgg taaatagagc
     2281 gcaatatcgg aaataacatc gccagtctca ttcaaaatcg cccccagacg tgtttgctga
     2341 ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat aaaaagcacg
     2401 atgggcaata gcaaaaagag gatgggttgt gccaccaaca ccaacagcaa tccggtaaaa
     2461 agagaaagcg ccagtgccgt aagagtgatg tgattcgctg taacgtggtg cttataaagc
     2521 caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta gagcgtcatt
     2581 atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttcccctgac ggagatatcg
     2641 caacacgccg cttcattaaa aattcagaat atatcaaggt attgggggct cactgtcagg
     2701 aatgattatc cccaataaat caagtagtta aaaattatta cgctgttatt caatacaact
     2761 catcaagttt cctcatcgct tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct
     2821 ggcggactaa atccagttca gggttaacaa gctctaacgc cagcaaagga tcacgtttca
     2881 gcctcgcctt caatacatca actggaaagc ggggattatt catcgccaca atccaccact
     2941 cgggatcaat ttctgccgcc agtgccgcag agggggtgtg gcggttgcta agtgcgccga
     3001 acttgaaaaa tgtgagattg cttttcgcca gtgtactcca ccatatacga tccggttgtt
     3061 ttgccagtac atccagttgt gctaacgcat cggggtcgag tgcataataa acacgtaatg
     3121 cttcatcaac ctgcgcacgg gagctatttt cctgcctcag aaaaaacttc gcccaaatat
     3181 cattgcgtgg cgtgttttgc tgtaattcct tgagaacaac agcgggtaaa ttatcccaac
     3241 taactaatgc actctctaaa aagtcccagt cttccaaacg tatcagggcg gcggcattta
     3301 atagcatctg gtcatcaact tcgccgtggc taatcagctc agacaaaatc atatcagcgc
     3361 tggtttcata tgcatctgaa tctgctttcg gattattttt ccgatgcgat aaagcacgtt
     3421 gccaaatttt ttgtttcatc gacgtgctca gatactgatt tttcgccaga ttactccacc
     3481 cctcaggatc gttgaaacgt gtcaataacc gttgtaaagc agcgtcagag tgaattacct
     3541 gcatatcccg acagcaaaag taggcagggg attttttctc catcctttta acaaaattaa
     3601 actgtaagtt atctggcaat gctaaaaata acacacgtat tagatcatcg tcattctcga
     3661 atttttggta taaataggct gcaactttgt cactctcttg tggcgtcatc cgcaataatt
     3721 gaccatggtg tttcatctct gccagcttga acgctaagct tgtgacaaca ttgtttgatg
     3781 aatcgttcgc caactgcatt gcctgattgt aagtgagttg tcctccagca ccgacctgac
     3841 gaatagagga atcatcactg cggatatagc catcaataat ggattcgggc aataaccacg
     3901 catctttata aagctcctga atgatcatat cccaatctct tttcgccaga gtcatgacga
     3961 tatccagagg gagattttca cgtagcgcaa gattcaacgg accgacacca tcaggtagac
     4021 gactcattcg cgtcattatt tcgacagaga gcggttcacg gtcgagcaac cccagaacaa
     4081 caggctggta acccaattgc tgatcggtca actccagaat ctcattgtca ggcatatatc
     4141 tcgccatact ttcaatcgtt tgttcatcat tgctattaat aagttgtttg cgtaggttag
     4201 ctgaaattcc tttaagcctt gggagagttc gcattatggc aagatcaaat gtcgcaatat
     4261 ctcgtccttc aaatgcagca acgttgcctt catccagcat ttgcaattcc tgcactaact
     4321 caggagaagg cttagggcga aacagcgctt cttttttcat ctgattacag acattccctg
     4381 ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc atcttcatca tttattagac
     4441 cccaataata tgcatcagga tttattatca tcagcgcttt tttgacattt tcagtgtcat
     4501 ttttgtcact cgcacggacc atgaattcac tatttgtcga agacgcaaca acaggtgcaa
     4561 ttatgtcatt gataaatacc ccacgcgcat taatactccc cttaatcgta gacggattat
     4621 cgtaatatga gtcgataaat accagtccac ttgcatttat cgaaccggta attaacagtg
     4681 gtgaatccgt aactatattc gttgccgtca cgttaccgag aataacccat ggattagaaa
     4741 gagagaaagt ggaaatatta cctttgactg tcaaattgcc aagaacaatc aatttttgat
     4801 tactctctat attaccttct accactaaat cgccacgatg cactttccag tccgatgcag
     4861 ataaaccgaa ggggacaaga ttcacgctac tgttgatagc ttgttcaact
//
LOCUS       DAJTYL010000188         4867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYL010000188 DAJTYL010000000
VERSION     DAJTYL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.190"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            188..1114
                     /gene="tauA"
                     /locus_tag="OO572_003778"
     CDS             188..1114
                     /gene="tauA"
                     /locus_tag="OO572_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6662605.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1127..1894
                     /gene="tauB"
                     /locus_tag="OO572_003779"
     CDS             1127..1894
                     /gene="tauB"
                     /locus_tag="OO572_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6662606.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1891..2718
                     /gene="tauC"
                     /locus_tag="OO572_003780"
     CDS             1891..2718
                     /gene="tauC"
                     /locus_tag="OO572_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6662607.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2715..3566
                     /gene="tauD"
                     /locus_tag="OO572_003781"
     CDS             2715..3566
                     /gene="tauD"
                     /locus_tag="OO572_003781"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6662608.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3672..4646)
                     /gene="hemB"
                     /locus_tag="OO572_003782"
     CDS             complement(3672..4646)
                     /gene="hemB"
                     /locus_tag="OO572_003782"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6662609.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1089 a         1249 c         1392 g         1137 t
ORIGIN      
        1 attaaaatac acggcgcgcc tatcttgaat gttttatcga tatcgaagca ttgagcattt
       61 ttaactcgtt ttatcttgaa tgcaatcggc gcataactta ttgaatccca tcaaatcccg
      121 tcttttttta cccattcccg gttatcttga ataaattacc tagtcatata acacacttct
      181 tgccgcactg gcattcatcg cttttcaggc acaggcggtg aacgtcaccg tggcgtatca
      241 aacctcagcc gaaccggcga aagtggctca ggccgacaac acctttgcta aagagagcgg
      301 ggcgagcgtg gactggcgta agtttgacag cggagccagc atcgtgcggg cgctggcttc
      361 tggcgacgtg caaatcggca acctcggttc cagcccatta gcggttgcag ccagccaaca
      421 ggtgccgatt gaagtcttct tgctggcgtc aaaactgggt aactccgaag cgctggtggt
      481 aaagaaaact atcagcaaac ccgaagattt gattggtaaa cgcattgccg taccgtttat
      541 ctccaccacc cactacagcc tgctggcggc gctgaaacac tgggggatta aaccagggct
      601 agtggagatt gtgaacctgc agccgcccgc gattatcgcc gcatggcagc ggggtgatat
      661 tgatggtgct tatgtctggg caccggcggt taacgccctg gaaaaagacg gcaaggtgct
      721 gaccgattct gaacaggtcg ggcagtgggg tgcgccaacg ctggacgtct gggtggtgcg
      781 caaagatttt gccgagaaac atcctgaggt cgtgaaagcg ttcgccaaaa gcgccatcga
      841 tgcacagcaa ccgtacattg ctaaccctga cgcgtggctg aaacagccgg aaaacatcag
      901 caaactggcg cgtttaagcg gcgtgcctga aggtgacgtt ccggggctgg tgaagggaaa
      961 tacctatctg acgccgcagc aacaaacggc agaactgacc ggaccggtga acaaagcaat
     1021 catcgacacc gcgcagtttt tgaaagagca gggcaaggtc ccggctgtag cgaatgatta
     1081 cagccagtac gttacctcgc gcttcgtgca ataaaaggag gcgcagatgc tgcaaatctc
     1141 tcatctctat gccgattatg gcggcaaacc ggcactggaa gatatcaacc tgacgctgga
     1201 aagcggcgag ctactggtgg tgctggggcc gtccggctgc ggtaaaacca ccctgctgaa
     1261 tctgattgcc ggttttgtgc cttatcagca tggcagcatt caactggcgg gtaagcgtat
     1321 tgagggaccg ggagcagagc gtggcgtagt ttttcagaat gaagggctgc taccgtggcg
     1381 caatgtacag gacaacgtgg cgttcggcct gcaattggca ggtatagaga aaatgcagcg
     1441 actggaaatc gcgcaccaga tgctgaaaaa agtggggctg gaaggcgcag aaaaacgcta
     1501 tatctggcag ctttccgatg gtcaacgtca gcgggtgggg attgcccgcg cgctggcggc
     1561 gaatccccag ctgttattac tcgacgaacc gtttggtgcg ctggacgcct tcacccgcga
     1621 ccagatgcaa actctgctgc tgaaactctg gcaggagacg ggcaagcagg tgctgttgat
     1681 tacccacgat atagaagaag cggtgtttat ggcgactgaa ctggttctgc tttcatccgg
     1741 ccctggccgt gtgctggagc ggctgcggct caactttgct cgccgctttg ttgcgggaga
     1801 gtcgagccgc agcatcaagt ccgatccaca attcatcgcc atgcgcgaat atgttttaag
     1861 ccgcgtattt gagcaacggg aggcgttctc atgagtgtgc tcattaatga aaaactgcat
     1921 tcgcggcggc tgaaatggcg ctggccgctc tcgcgtcagg tgaccttaag cattggcacg
     1981 ttagcggttt tactcactgt atggtggacg gtggcggcgc tgcaactgat tagcccgctg
     2041 tttttgccgc cgccgcaaca agtactggca aaactgctca ccattgccgg accgcaggga
     2101 tttatggacg ccacgctgtg gcagcatctg gcagccagcc tgacgcgcat tgtgctggcg
     2161 ctactcgcgg cggtggtgat cggtattccg gtcgggatcg ccatggggct aagcccgacg
     2221 gtgcgcggca ttctggatcc ggtaatcgaa ctttatcgtc cggtgccacc gctggcttat
     2281 ttgccgctga tggtgatctg gtttggtatt ggtgaaacct cgaagatctt actgatctat
     2341 ttagcgattt ttgcgccggt ggcgatgtcg gcgctggcag gggtgaaaag cgcgcagcag
     2401 gttcgcattc gtgccgccca gtcgctgggt gccagccgtg cgcaggtgct gtggtttgtc
     2461 attttgcccg gcgcgctgcc ggaaatcctc accggattac gcattggtct gggtgtgggc
     2521 tggtctacgc tggtagcggc ggagctgatt gccgcgacac gcggtttagg atttatggtt
     2581 cagtcagcgg gtgaatttct cgcgactgac gtggtactgg cggggatcgc ggtgattgcg
     2641 attatcgcct ttcttttaga actgggtctg cgcgcgttac agcgccgtct gacgccctgg
     2701 catggagaag tacaatgagt gaacgtctga gcattacccc gctggggccg tatatcggcg
     2761 cacaaatttc gggtgccgac ctgacgcgcc cgttaagcga taatcagttt gaacagcttt
     2821 accatgcggt gctgcgccat caggtggtat ttctgcgcga tcaagctatt acgccgcagc
     2881 agcaacgcgc gctggcccag cgttttggcg aactgcatat tcaccctgtt taccctcatg
     2941 ccgaaggggt tgacgagatc atcgtgctgg atacccataa cgataatccg ccggataacg
     3001 acaactggca taccgatgtg acatttattg aaacgccacc cgcaggggcg attctggcag
     3061 ctaaagagtt accttcgacc ggcggtgata cgctctggac cagcggtatt gcggcctatg
     3121 aggcgctctc tgttcccttc cgccagctgc tgagctggct gcgtgcggag catgatttcc
     3181 gtaaatcatt cccggaatac aaataccgca aaaccgagga ggaacatcaa cgctggcgcg
     3241 aggcggtcgc gaaaaatcct ccgttgctac atccggtggt gcgaacgcat ccggtgagcg
     3301 gtaaacaggc gctgtttgtg aatgaaggct ttactacgcg aattgtcgat gtcagcgaga
     3361 aagagagcga agccttgtta ggctttttat tcgcccatat cactaaaccg gagtttcagg
     3421 tgcgttggcg ctggcaaccg aatgatattg cgatttggga taaccgcgtg actcagcact
     3481 atgccaatgc cgattacctg ccacagcggc ggattatgca tcgggcgacg atccttgggg
     3541 ataaaccgtt ttatcgggcg gggtgatacg agtgcggacg gttccctcgc ccccttgggg
     3601 agagggttag ggtgaggggg cgttcactgt actttcaaca ggttaactcc ccctttctga
     3661 gaggaaaaaa attaacgcag aatcttcttc tcagccaaat ccagcgcaaa gtagctgaaa
     3721 atcagatccg cacccgcacg cttaattgaa cctaagcttt cgagcacgac tttctcttca
     3781 tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct cacctgatac
     3841 gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag gtacgctcca
     3901 gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga ctcacgaatc
     3961 gcctcgcgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt taatgcgctt
     4021 cctgcagctt cacggaacgg gccataaaag gaagaggcga acttggtcga atacgacata
     4081 atcgccgtat ctttaaagcc tgcagcgtcc agcgcctgac gaatcgcctg tacctggccg
     4141 tccatcgcgg cggaaggggc gatgaagtct gcgcctgcag cagctgcaac cacggcttgc
     4201 ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag cacaccgcag
     4261 tgaccgtgag aagtgtattc acagaaacaa gtgtctgaca tgacgatcat ttctggcaca
     4321 gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca ggcgtcgctg
     4381 ccggtttcat cggtatggtg agagatgcca aaagtcatta cggaacggat accggcgttg
     4441 gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac gcctggcatg
     4501 gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa agatcggcaa caccaggtcg
     4561 ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt gcgcaggcga
     4621 cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaatt aaggtgcaca
     4681 gtatacctga agcggggcag gagtgtttta cgaaagttgt cgcgatgttg acaagaagag
     4741 aatgaaagag agaccaggac atctttgtaa tagaaatagc aataatttca tattcactga
     4801 aaatattttt aatctttatt tatagctctt ggttattatt ttttggcgtt tggttgtgct
     4861 gctataa
//
LOCUS       DAJTYL010000189         4701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYL010000189 DAJTYL010000000
VERSION     DAJTYL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.191"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            160..1575
                     /gene="tnaA"
                     /locus_tag="OO572_003783"
     CDS             160..1575
                     /gene="tnaA"
                     /locus_tag="OO572_003783"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6662610.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1666..2913
                     /gene="tnaB"
                     /locus_tag="OO572_003784"
     CDS             1666..2913
                     /gene="tnaB"
                     /locus_tag="OO572_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6662611.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3045..4220
                     /gene="mdtL"
                     /locus_tag="OO572_003785"
     CDS             3045..4220
                     /gene="mdtL"
                     /locus_tag="OO572_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6662612.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4195..>4701
                     /gene="yidZ"
                     /locus_tag="OO572_003786"
     CDS             4195..>4701
                     /gene="yidZ"
                     /locus_tag="OO572_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6662613.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLA"
BASE COUNT         1056 a         1141 c         1186 g         1318 t
ORIGIN      
        1 tgctatatct taattttgcc ttttgcaaag gccatctctc gtttatttac ttattttagt
       61 aaatgatggt gcttgtatat atacatggtg aattaatgga tattgcagat gtaatattca
      121 cagggatcac tgtaattaaa ataaatgaag gattatgtaa tggaaaactt taaacatctc
      181 cctgaaccgt tccgcattcg tgttattgag ccagtaaaac gtactactcg cgcttatcgt
      241 gaagaagcaa ttattaaatc cggtatgaac ccgttcctgc tggatagcga agatgtgttt
      301 atcgatttac tgaccgacag cggcaccggg gcggtaaccc aaagtatgca ggcagcgatg
      361 atgcgcggcg acgaagccta cagcggcagc cgcagctact atgcgttagc cgagtcagtg
      421 aaaaatatct ttggttatca atatactatt ccgactcacc agggccgtgg cgcagagcaa
      481 atctatattc cggtactgat taaaaaacgc gagcaggaaa aaggcctgga tcgcagcaaa
      541 atggtggcat tctctaacta tttctttgat accacgcagg gccatagcca gattaacggc
      601 tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata cgggcgtgcg ttacgacttt
      661 aaaggcaact ttgacctcga aggattagaa cgcggtattg aagaagttgg cccgaataac
      721 gtgccgtata tcgttgcaac catcaccagt aactctgcag gtggtcagcc ggtttcactg
      781 gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg atattccggt ggtaatggac
      841 tccgcacgct ttgctgaaaa cgcctatttc atcaagcagc gtgaagcaga atacaaagac
      901 tggaccatcg agcagatcac ccgcgaaacc tacaaatatg ccgatatgct ggcgatgtcc
      961 gccaagaaag atgcgatggt gccgatgggc ggcttgctgt gcatgaaaga cgacagcttc
     1021 tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc aggaaggctt cccgacatat
     1081 ggcggcttgg aaggcggcgc gatggagcgt ctggcggtag gtctgtatga cggcatgaat
     1141 ctcgactggc tggcttatcg tatcgcgcag gtacagtatc tggtcgatgg tctggaagag
     1201 attggcgttg tctgccagca ggcgggcggt cacgcggcat tcgttgatgc cggtaaactg
     1261 ctgccgcata tcccggcaga ccagttcccg gcacaggcgc tggcgtgcga gctgtataaa
     1321 gtcgccggta tccgtgcggt agaaattggc tctttcctgt taggccgcga tccgaaaacc
     1381 ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg cgcaacatat
     1441 actcaaacac atatggactt cattattgaa gcctttaaac atgtaaaaga gaacgcggcg
     1501 aatattaaag gattaacctt tacctacgaa ccaaaagtat tgcgtcactt caccgcaaaa
     1561 ctgaaagaag tttaattaat actacagagt ggctataagg atgttagcca ctctcttacc
     1621 ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac tgatcaagct
     1681 gaaaaaaagc actctgcatt ttggggtgtt atggttatag caggtacagt aattggtgga
     1741 ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg tgcctttatc
     1801 cttatcattg cctggttttc aatgcttcat tccgggttat tgttattaga agcaaattta
     1861 aattatcctg tcggctccag ttttaacacc atcaccaaag atttaatagg taacacctgg
     1921 aacattatca gcggtattac cgttgccttc gttctctata tcctcactta tgcctatatc
     1981 tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta tcacgctaat
     2041 ccacgtattg tcgggatctg cacagccatt ttcgttgcca gcgtattgtg gataagttcg
     2101 ttagccgcca gtcgcattac ctcattgttc ctcgggctga agattatctc ctttgtgatc
     2161 gtgtttggtt ctttcttctt ccaggtcgat tactccatcc tgcgcgatgc caccagcacc
     2221 actgcgggaa cgtcttactt cccgtatatc tttatggctt tgccggtgtg tctggcgtca
     2281 tttggtttcc acggcaatat tcccagcctg attatttgct atggcaaacg caaagataag
     2341 ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg tgatttatct cttctggctc
     2401 tattgcacga tggggaatat tccgcgcgaa agctttaagg cgataatctc ctcaggcggc
     2461 aacattgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat tatcgagttt
     2521 tgcctgctgg tgttctctaa cttagctgtt gccagttcgt tctttggtgt cacgctgggg
     2581 ttgttcgatt atctggcgga cctgtttaag attgataact cccacggcgg gcgtttcaaa
     2641 accgtgctgt taaccttcct gccacctgcg ttgttgtatc tgatcttccc gaacggcttt
     2701 atttacggga tcggcggtgc cgggctgtgc gccaccatct gggcggtcat tattcccgca
     2761 gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga tgttcacggt ctggggcggc
     2821 aatcttattc cggcgattgt cattctcttt ggtataaccg tgattttgtg ctggttcggc
     2881 aacgtcttta acgtgttacc taaatttggc taaatccttc aagaagccag ccattcgctg
     2941 gcttcttgcc tctcaggaaa tcacttatgt ccaaatggca actcgcctta tcctccttca
     3001 ccacgtatgc tttgcgtcac cttactatca ggacgcttta gcccatgtcc cgctttttga
     3061 tttgtagttt tgccctggtt ttactttatc ccgccgggat tgatatgtac ctcgttggtt
     3121 taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg
     3181 tatatctggc ggggatggca gctgcgatgc tatttgccgg taaagtggcc gatcgttcag
     3241 ggagaaagcc ggtcgccata cccggcgcgg cgctatttat tattgcctcg gtgttctgtt
     3301 cactggctga aaccagcacg ttatttcttg caggccgatt tctacagggg ttgggcgcag
     3361 gctgttgtta cgtggtggcg ttcgccattt tgcgcgacac gctggatgat cgacgtcggg
     3421 ctaaagtgct gtcattactc aacggtatta cctgcatcat tccggtgtta gcgccagtgc
     3481 tcggacatct gattatgctt aaattcccgt ggcagagtct gttctgggcg atggcaatga
     3541 tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa agaaacgcgc ccagcgtccc
     3601 ccgctgcttc ggacaaacca cgagaaaata gcgagtcgct gcttaatcgt tttttcctca
     3661 gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct cactttcgtc aatacatcgc
     3721 cggtattgct gatggaaatc atggggtttg agcgcggaga atacgccacc attatggcgc
     3781 tgaccgctgg cgtcagcatg accttttcat tctcgacgcc atttgcgctg ggaattttta
     3841 agccacgtac gttgatgatc acctcgcagg tgttattcct tgcggcgggg atcacccttg
     3901 ccgtttcacc ttcccatgcg gtttctctgt ttggtatcac gctgatttgc gccggtttct
     3961 cggtaggttt tggcgtagcg atgagtcagg cgttaggacc attttcatta cgcgcgggcg
     4021 tagccagctc gaccttaggt attgcgcagg tttgcggttc gtcactgtgg atttggctgg
     4081 ccgcggtggt tggtatcggc gcatggaata tgctgatcgg gattctgatt gcctgtagca
     4141 tagtgagcct gttgctgatt atgttcgtcg cgcctggacg ccccgttgcc gctcatgaag
     4201 aaatccatca ccacgcttga tctcaatctg ctgctctgtc tgcaactgct gatgcaggag
     4261 cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc cttcagcggt gagtaagtcg
     4321 ctggcaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc gctggggctg
     4381 tcgcccacac agctaatggt cagcatggag caaaatctgg cggaatggat gcaaatgagc
     4441 aatctgctgc tggataaacc gcaccaccag acaccgcgcg gcctgaagtt tgagttggcg
     4501 gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt cgaaacggat ctaccaacgt
     4561 tacccgcagg cgaccatcaa actacgtaac tgggattacg attccttaga tgccattact
     4621 cgtggtgaag tggatattgg tttttccggt cgcgaaagcc atcctcgctc gcgggagctg
     4681 ttaagctcgc taccgttagc c
//
LOCUS       DAJTYL010000190         4615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYL010000190 DAJTYL010000000
VERSION     DAJTYL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.192"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(678..1094)
                     /locus_tag="OO572_003787"
     CDS             complement(678..1094)
                     /locus_tag="OO572_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662614.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1121..1261
                     /locus_tag="OO572_003788"
                     /pseudo
     CDS             1121..1261
                     /locus_tag="OO572_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1261..2286
                     /locus_tag="OO572_003789"
     CDS             1261..2286
                     /locus_tag="OO572_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662615.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2339..2428)
                     /locus_tag="OO572_003790"
     tRNA            complement(2339..2428)
                     /locus_tag="OO572_003790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ser,seq:cga)
     gene            2522..3319
                     /gene="mtfA"
                     /locus_tag="OO572_003791"
     CDS             2522..3319
                     /gene="mtfA"
                     /locus_tag="OO572_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6662616.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3420..3495
                     /locus_tag="OO572_003792"
     tRNA            3420..3495
                     /locus_tag="OO572_003792"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3453..3455,aa:Asn,seq:gtt)
     gene            3657..4615
                     /locus_tag="OO572_003793"
                     /pseudo
     CDS             3657..4615
                     /locus_tag="OO572_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1266 a         1093 c         1019 g         1237 t
ORIGIN      
        1 attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cacgtaagag
       61 gctaacctac atgtgttcag catggattga gcctcagatt aatgttaagc gtcttgcagg
      121 acgcgtaatg ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgagacag
      181 atagcctcaa gcacccgcag tcattctact taactaagat ttccctgcaa accgtttttg
      241 tccggcacag taaatatcca actaaaccaa tagcgttcgc tgtatttacc gccagtattc
      301 aatgcacatg accgccatga acacccctaa aaaaagggca tttatatgtc caaacattaa
      361 tatcaaaaca tcaatttttt ccatatacct tgctgtgaag atgatgggca tacatgatgc
      421 gaacaaccag aacgcaacaa acaaaaactg caatgcgttt ttcattattc cccctacaat
      481 caatgtgcaa taacatttaa acacacctca atttggccgg acatataaat atctaaacca
      541 gaaaaaatca cttacatagc gttacaaact ctttagtcta aatactcatc gtaaaacatt
      601 ccccatactt atcagtccgt tccgcgccag gtagcttatt gccttatctg gcaacctata
      661 atcaggtttc cgctttttca gttggctggt cgtttaaccg acatagttaa cccattaatc
      721 tggttgccgg atgctggtgg attttcgcgt tttaattgtt cataaaagtg cacagcttta
      781 accagttctt ctgatgtaac cgggactggt ggggcagtga ataaggcctg aatttcatag
      841 ttcggcctgt cgttacaatc ctcttttttc ggtacatatt tccagtcacc aacccacaac
      901 tccccctgag agtccataac accttttttc acgtagcgat atcgccacgc tatcggctct
      961 gcttccagcg atgcccgtgc aattttgaat aactcgccct ctactcgcgc catccctgaa
     1021 ttggggtggc atttcgtaat cgctattttt aatttggctt cttcgattaa ttgttctttt
     1081 gttaattcag tcattttcat taccgccctg gtaatgctgc caccagcgaa aatggttaat
     1141 ggaaaacggg cttccgttct ataccaatcg tagtggcaaa ccgattgtca gccgggatct
     1201 atttacctgc aataaaactt taccaccacg cgaggtagag ccgaattttg gtgcgatctg
     1261 atgggaagac gaaggaaaaa tcctgaacac gaaaaattac ctccaaatgt atacccaaat
     1321 aaatatagtt atgtatggaa accaacatcc agagaatctg taacactaac cgccatcaag
     1381 gatggtttag ctgctttatg gaaaaagtat gaggaaactg taaataatcg cgatcgtgca
     1441 atgacattcg gtcgcttgtg ggaaaaattc ctcgccagcg cctattacag tgacctcagt
     1501 ccaagaacac aaaaagatta tctgcaacat caaaaaaagt tgcttgccgt attcggtaag
     1561 gtgccggcag attccataaa accagaacac atccgtcgat acatggacaa gagaggggaa
     1621 cagagtaaaa cgcaagccaa ccatgaaaaa agcagtatgt cccgcgttta cagttggggg
     1681 tatgagcgag ggtacgtgaa ggctaaccca tgtgcaggtg taagtaaatt caaggccaaa
     1741 aaccgcgaac gatatgtaac cgacaaagaa taccaggcag tattaagcgt tgcacctctt
     1801 cctgttttta tcgcaatgga aattgcctat ctgtgtgcag cgagggtttc cgatgtgtta
     1861 tcgctgaaat gggagcagat tggaaacgac gggatcttta tccagcaagg gaaaacagga
     1921 aaaaaacaga taaaagcatg gagtccacga ttacaggcgg cgatcgaaaa agcaaaacag
     1981 ttaccaacat ccgcctatgt aatcagcaat caatacggca accgatatat gtacaaaggc
     2041 tttaacgaaa tgtgggtaga agcaagaaat cgcgcaggca aaatttcagg tattttaacc
     2101 gacttcacct ttcatgatct gaaggcgaaa ggaatttcag actatgaagg aagcagtcgg
     2161 gataagcaac ttttctctgg tcacaaaacc gaggggcaag tgctaatcta tgacaggaag
     2221 gttaaagttt caccgacact tgatgtcccg ttacctgaaa atattccaag aaaatattcc
     2281 aagtaattcc aagtgtgatt tttgtcactg acttaatgat gtataagtga ttgaattttg
     2341 gcggagagag ggggatttga acccccggtg gagttgcccc cactccggtt ttcgagaccg
     2401 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
     2461 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
     2521 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
     2581 ggaagcacta tcgatccccc ttttaacgtg tctgacagaa caggaacaaa gcaaattggt
     2641 cgctcttgcc gaacgttttt tacagcaaaa acggcttgtt cctttacagg gctttgagct
     2701 gaattcatta agaagctgcc ggatagcact tctattttgc ctgcccgttc tggagttagg
     2761 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
     2821 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
     2881 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
     2941 ttctggtttt aacctgatta ttcatgaagt cgctcataag ctggacaccc gtaacggcga
     3001 tcgcgccagc ggagttccct ttatttcgtt gcgtgaggtt gctggctggg aacacgatct
     3061 tcatgctgca atgaacaaca ttcaggaaga aatcgaatta gttggtgaga atgcggcgag
     3121 cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     3181 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgtca
     3241 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     3301 agcgacgaat gttcattaat taacaacttt gaagattaat taaccaattg aaatggctta
     3361 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tctcacgatt
     3421 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     3481 cagtcagagg agccaatttt ctgttttcat gcatccttgc gaatctttat ctgatgttga
     3541 ttcaacaggt tagtgtgaaa actcttctcg gtgcattttg attttaccct acgcatcggg
     3601 aaaaattggt ggtcaaatct gggatcaggt tagttcgata atggagtgac ccccatatat
     3661 cccttaccga cgcaaaaatc cgcaccctca agccttctga taaacccttt aaagtctccg
     3721 attctcacgg tctgtatctg ctggtcaagc cgggtggctc ccgccactgg tatctcaaat
     3781 accgtattag cggtaaagaa tcccgcattg cgctgggtgc ctatccagcc atctccctgt
     3841 ctgatgcgcg acagcaacgt gaaggtatcc gtaaaatgct ggcgctgaat atcaacccgg
     3901 tacagcagcg ggctgctgaa cgtggctcac gaacaccgga gaaagttttt aaaaacatgg
     3961 cgctggcgtg acataaaagt aacaggaaat ggtcgcagaa caccgccgac cgtctgcttg
     4021 ccagcctgaa caatcacatc tttccggtca tcgggaacct acctgtatca gaacttaaac
     4081 cccgtcattt cattgacctg ctgaaaggga tcgaggaaaa aggtctgctg gaggttgcgt
     4141 cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag gagttaatcg
     4201 atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga cggcactatc
     4261 ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg tattggggca tatcatcagg
     4321 gccgtgaact gacccggcat gccgttctgc tgatgctgca tgtgttcatt cgctccagtg
     4381 aactgcgttt cgcccgctgg tcagagattg atttcacaaa ccgagtctgg acgatacccg
     4441 cgacgcgaga acccattatt ggcgtgcgtt attccggccg cggggcaaaa atgcgaatgc
     4501 cgcatatcgt ccccctctca gaacagtcca tcgccattct gaaacagatt aaggatatca
     4561 ccggtaataa tgaactgatc ttccccggcg accataaccc gtataagcca atgtg
//
LOCUS       DAJTYL010000191         4594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYL010000191 DAJTYL010000000
VERSION     DAJTYL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.193"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..212
                     /locus_tag="OO572_003794"
                     /pseudo
     CDS             1..212
                     /locus_tag="OO572_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            355..576
                     /locus_tag="OO572_003795"
                     /pseudo
     CDS             355..576
                     /locus_tag="OO572_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(597..659)
                     /locus_tag="OO572_003796"
                     /pseudo
     CDS             complement(597..659)
                     /locus_tag="OO572_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1109..2521
                     /gene="ospC1"
                     /locus_tag="OO572_003797"
     CDS             1109..2521
                     /gene="ospC1"
                     /locus_tag="OO572_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6662617.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2625..2774)
                     /locus_tag="OO572_003798"
                     /pseudo
     CDS             complement(2625..2774)
                     /locus_tag="OO572_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2850..4547
                     /gene="ospD3"
                     /locus_tag="OO572_003799"
     CDS             2850..4547
                     /gene="ospD3"
                     /locus_tag="OO572_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6662618.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1606 a          821 c          853 g         1314 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatgggggcg
      121 gtgaagaatt atctaacctt gtcttgttac atccaaattg ccatcgacag ttacacagtt
      181 gtgaagccgg ctctctcaaa aagggcttat aaaggcttga gcggtgtacc gggaaaccgg
      241 tatgcaccgt tcttagggga gggcagtcca gcaatgaact gtccttaccc gacaaacatc
      301 caaaacgaac ggaacgtgct ggaatccgca tataacccgt tgtaaagtga ggaaatggac
      361 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacact
      421 atccaccaga tggctgaata caaccgcctg ctctcacagc ggatggcggc ttatgcttcc
      481 gaaatcaacc gactgaaggc gctggttgcg aaactgcagc gtatgcagtt cggtaaaagc
      541 tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat tttcacgggc gatctacgcc
      601 acacaggctc tgggttgcag tgactgctcc attaaggcga ttttgaactc ataagggaag
      661 taacaaaaaa actgcaaaag tacaataatt atatatggcc aaactaacct ctattaaaaa
      721 acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat ttcattgcag
      781 aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag tatcgttttt
      841 ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt atgaaaataa
      901 tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt ttgaaatatc
      961 tggcatcaca taacatcaaa agtatataat actcttatct aattacatgc agtatattgt
     1021 atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa gatattaatt
     1081 aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga actcagcaaa
     1141 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
     1201 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
     1261 aaatattatt aaaagtttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
     1321 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
     1381 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
     1441 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
     1501 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
     1561 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
     1621 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
     1681 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
     1741 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
     1801 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
     1861 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
     1921 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
     1981 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
     2041 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
     2101 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     2161 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     2221 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     2281 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     2341 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     2401 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     2461 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     2521 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     2581 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     2641 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     2701 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
     2761 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     2821 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     2881 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     2941 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     3001 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     3061 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     3121 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     3181 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     3241 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     3301 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     3361 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     3421 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     3481 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     3541 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     3601 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     3661 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     3721 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     3781 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     3841 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     3901 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     3961 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     4021 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     4081 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     4141 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     4201 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     4261 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     4321 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     4381 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     4441 tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     4501 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     4561 gtgatacact gaatttgagg taaccggctc attt
//
LOCUS       DAJTYL010000192         4575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYL010000192 DAJTYL010000000
VERSION     DAJTYL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.194"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..575)
                     /locus_tag="OO572_003800"
     CDS             complement(<1..575)
                     /locus_tag="OO572_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR6662619.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
     gene            complement(690..765)
                     /locus_tag="OO572_003801"
     tRNA            complement(690..765)
                     /locus_tag="OO572_003801"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:ggt)
     gene            complement(772..846)
                     /locus_tag="OO572_003802"
     tRNA            complement(772..846)
                     /locus_tag="OO572_003802"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(811..813),aa:Gly,seq:tcc)
     gene            complement(963..1047)
                     /locus_tag="OO572_003803"
     tRNA            complement(963..1047)
                     /locus_tag="OO572_003803"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1011..1013),aa:Tyr,seq:gta)
     gene            complement(1056..1131)
                     /locus_tag="OO572_003804"
     tRNA            complement(1056..1131)
                     /locus_tag="OO572_003804"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Thr,seq:tgt)
     gene            1493..2443
                     /gene="coaA"
                     /locus_tag="OO572_003805"
                     /gene_synonym="panK"
     CDS             1493..2443
                     /gene="coaA"
                     /locus_tag="OO572_003805"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6662620.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2472..3437)
                     /gene="birA"
                     /locus_tag="OO572_003806"
     CDS             complement(2472..3437)
                     /gene="birA"
                     /locus_tag="OO572_003806"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6662621.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3434..4462)
                     /gene="murB"
                     /locus_tag="OO572_003807"
     CDS             complement(3434..4462)
                     /gene="murB"
                     /locus_tag="OO572_003807"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6662622.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1165 a         1154 c         1037 g         1219 t
ORIGIN      
        1 agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag agcagaacca
       61 cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc acgaacttcc
      121 atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg
      181 atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc
      241 gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc accggtgatc
      301 atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg
      361 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
      421 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      481 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      541 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      601 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      661 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      721 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      781 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      841 gcccgcatcc tagaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      901 ctctttattc gagccggtaa gtgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      961 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
     1021 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
     1081 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
     1141 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
     1201 tggtcacatt ttatgctaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
     1261 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
     1321 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1381 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1441 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1501 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1561 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1621 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1681 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1741 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1801 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1861 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1921 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1981 caaatccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     2041 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     2101 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ccgattttgt
     2161 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     2221 ttttctgaaa ttccgcgaag gggcttttac agacccggat tcctattttc ataactacgc
     2281 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     2341 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2401 aagtgctaat catgtggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2461 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2521 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2581 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2641 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2701 aattcacgta ttagcatggc cgccaacgta ttacgatcaa gattgatccc cgcttcctgc
     2761 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2821 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2881 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2941 gctcccagct ttcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     3001 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     3061 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     3121 gcatcgcccg atttaagctc tccgatacga tcaagaagga actgattcgt ggagtcaata
     3181 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     3241 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     3301 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3361 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3421 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3481 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3541 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3601 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3661 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3721 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3781 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3841 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3901 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3961 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     4021 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     4081 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     4141 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     4201 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     4261 tgatccggtt gatgatcacc gtgccgcgat agtcttccag aaaaagtaca ttacttcctt
     4321 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4381 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4441 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacgaggc tagtttaccg
     4501 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4561 ggcccgactg ggctt
//
LOCUS       DAJTYL010000193         4516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYL010000193 DAJTYL010000000
VERSION     DAJTYL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.195"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..758)
                     /locus_tag="OO572_003808"
     CDS             complement(<1..758)
                     /locus_tag="OO572_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6662623.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGV"
     gene            complement(770..1259)
                     /locus_tag="OO572_003809"
                     /pseudo
     CDS             complement(770..1259)
                     /locus_tag="OO572_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1263..2483)
                     /locus_tag="OO572_003810"
     CDS             complement(1263..2483)
                     /locus_tag="OO572_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6662624.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2487..3230)
                     /locus_tag="OO572_003811"
     CDS             complement(2487..3230)
                     /locus_tag="OO572_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6662625.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3121..>4516)
                     /locus_tag="OO572_003812"
     CDS             complement(3121..>4516)
                     /locus_tag="OO572_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6662626.1"
                     /translation="RVGMQTPNQHSASTYRANFTSRNRMLVEWSYRSSWIIGEAVDAI
                     PDDMTRKGIRITSEIDAKDRGILESQLDELQIWDALNDVLKWSRLYGGAVGFIMIEGQ
                     APMTPLRPETIGKGKFKGILPLDRWMVDPALTRRIKDMGPDLGKPEFYDVVTTATGIP
                     AWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAH
                     LRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGL
                     DDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGDADLANYYDRISSLQERRLRLPVRR
                     ILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRSTVALNTTNAISTALGDGLMTLKAA
                     MTDLRENSDVTGIGASITNEDIENAEDEAPPGIGEPDDEPQEPSGGNPVSNQPTQDSE
                     GGRRHRKWSLRWFK"
BASE COUNT          953 a         1286 c         1149 g         1128 t
ORIGIN      
        1 acaccacggc ctttcagcca tttaaccgcg cggatattca gcggaacacc gttctggtga
       61 tagctgatgg tgttggtctg aaggttcgtc aacagggact ggttacctgc agatgaaacg
      121 atgatgctgg acaacagagc aaactgctca ggcgggatca gcaaatcacg cggaaccaca
      181 gagtaaccag aagcggccca cgcatcagac agcacctggt taatgcttgc gcggatttcg
      241 tccggtgttg aggttgccca cgttttggca gcgttgttga caggcacgcc gtccagggta
      301 acaaggcctt tcaggtttaa tgcggaatcg ccaacatata cctgttcatc gttatccatc
      361 tgccatttca gttgcatacc gtcatacttc tgcgtatcaa tcgggcggcc gacctgctga
      421 gcagcctgca attctatgac cgtccagcca agttccatcc cccacaggtt cagcgggtta
      481 ccggatttgc cgatatccac gttcacgcca gcaatagcgg ttgagtcttt gcctacccag
      541 ttttttccat tcggatttgc gccagtaccc gcagcggcga agctggtatt cgtccagctg
      601 gaaatgtcat ctgcgatgga ggcgtcttca cgcagttgaa tatcgcggct ccaggtgtac
      661 cccaccagtg gcaggttcag cgtctggtcg agtctctcca gctccccgat gagaaaggca
      721 ccagagctgt caacggttgc ctgatcaaaa gtaatcattc gtctgttcct taaatcttcc
      781 aggaaatttc tgcattgccg tcagcatcac cggcacctgt gaattcagcg ttggtcagca
      841 ccacgttttt gccactgact gacgtggaca tgaatccacc cagaggcact ttgatggatt
      901 catcagtgga gatcgccttt tttgatagtg ctggcatcaa aatcagaacc gagattaacg
      961 gtcacgtagc cacgcttcat ggcgtcgccc gggaagttct tgcctgttcc cacctggcga
     1021 accatgtccg gctgcgacgt ggtcggataa gggcgcacgt agatcccctt caccttgtct
     1081 gcggtatcac catctgccag cggcacgaaa aaaccgtcat catcgtattt accagccagg
     1141 ccataggcag cgaaggcgtt atcggattta aggaccaccg gttcgacggt taagtcctgc
     1201 gggcgagaga cagccccggc aatgccaaca ggcatccggt acagaaatac attattcatt
     1261 ttttaccctt tacggtttgc ccagaattca gcgttttgtt tgttcaggga agcgatactg
     1321 gtcatgccca tgtttaggcg ctgtgcatcg ccggtggtgg cgcgggtgtt tcgccctttg
     1381 gcaacctcag acacggcatt aaacgccatg tcgaccgatt gtttcggtaa tttgcggata
     1441 tccgcatcac cgactatctg gcgaaccagc gttttgtcag cagaagccag aacctcgcgt
     1501 ttgaacgcgg tcggtttcat cttacggctc agatcgatac ccggaacgat aacttcggca
     1561 cgccaggctg agtcaccagt aatcgtggtt tcctcttcat cgtcctcgcc gtcaccggtc
     1621 ggattatcgt caggcttatt atcgttatcg cccgtggcat ttctttccag cttagccagc
     1681 agggctttca gtaatgtttt gaggtcatca tcactgtcgc cggttggatc tccgcccatc
     1741 tctggtgctt tgtccggtag tggttgctgc ggggacaggt tgatgttgag attaacgccc
     1801 tgcggcaaat ccccctcatc tcctgtaacc gatgcgggag ccgactccac cagttcgttc
     1861 atggtgtcgg catctcctgt cttgatggcc gcacgcatgc ggttccacca gtttttcttt
     1921 tgatttgcca ttgtgtctct gtctccaatt gcacaacgat ttccggctct gcctttaggg
     1981 acaagagcca catggtttcc ggtaatatcg acctgctcgg ctttacctgg ctcggtctgc
     2041 tcgtactccg cgtcatagcc gcacgacact tcgcgcaggc catcttcgat aagctgaatg
     2101 gcgctttcgt ctttgacgat aaggtcagcc agcatcaaat cagactgctc acccgtcccg
     2161 cgccggacat tctggaggtg cccgacagca agcactttcc agttctcggg atttaccagc
     2221 cgcacattcc cgttttcatc ttcaggatgc aggatcgtga tgctcatccc ttcgaatgag
     2281 gcaagcgtgg ccggatggaa tacctgctca ggagaacgcg tgacgactat ttcaacgaac
     2341 ttatcgggtt tcagttttgg caggtcatca gcaccataga gctgcttacc tgttcgtcct
     2401 atcggcacgt ctttgcacag caacgagccg tcagccaggc ggtatcgggt ttccccgagc
     2461 ctggtctcaa aaaagtattt catgggttat gctgcccttt tcattgcgaa tggaacgcga
     2521 acaaacacga tctctttata gcagcggcag tttggcagct cgcccgcgtg gccggtcatg
     2581 ccgtccaaag ttggaggtga atcccagcgg acgaatttac ctttcatgtg atcgtgggaa
     2641 tctcggacat cgccatcatc ggctgtacgc caaatataac catccgagcc aatagctatc
     2701 gctcgggcca tatccagcgc gcccgttgct cttcccagtt ccgttcgggc aatcagatcg
     2761 gccctagact tagcaacatc gccggtagat gcaatctctt tagcaaactg ctccgcccga
     2821 ccaccggtga caacagcctc tatcgctttg ttctggatgt cgtacaccct gtctgcggcc
     2881 tcgagaggca gagatttgat gtacttgacc tgttcggcga tgatggattg catcacctgg
     2941 cccacaggag cgctttccac aagattgcgg agctcgcgac tgatgttctt gctgtgttgc
     3001 cgccaaactt tctcgttctg ccgggttagg tccgcagtaa agttttccgc gacctttgtc
     3061 gcccaggggg tgatgatttc actgtaacgc tccagcgccg caataatttc cgtgatactg
     3121 tcatttgaac catcgtagcg accatttacg atgtctccga ccgccctcgc tatcctgcgt
     3181 aggctggttc gataccggat ttccgcctga cggctcctgc ggttcgtcat caggttcgcc
     3241 gatgccgggc ggcgcttcat cttcggcatt ctcgatgtcc tcgttggtaa tggatgcccc
     3301 tatgccggtt acgtcagaat tttctcgcaa atcagtcata gcggctttca gtgtcatcag
     3361 accatcaccc agcgccgtac tgattgcgtt ggtggtgttt aacgccaccg ttgagcgatc
     3421 gacatcagac atttgccaga gcgggttaaa ctcaaacgtg aaatcgtccg gcagcggctt
     3481 gccaagttcc gaacgatgca tgatgtccag tatccgccgc accggaagac gtaaacgcct
     3541 ctcctgcaac gaactgatgc gatcgtaata gttggcaagg tctgcatcac cggtagaaaa
     3601 tcctttcggg gactgtccga acaaccgcac cagtgggata ccaacagcgc cactaatctg
     3661 ttctgcaaac tgcgaaagga tgtcatccag accactgaag ctgtactgat gggtttcaaa
     3721 cttatcccgc gagtccatga gcgtcatgcc ttcattactc tggaactgtc gaatcaggtc
     3781 gatattcttc agcaacgctt catacgcagg accaccaagt gcgataagct cgcgtagctt
     3841 ctccacgctg taggtacgca aatgcgcctt gtagaccagc tgcgccgcgc cgacagtagc
     3901 gctgtcgaac gcggtaagac gatcccagat acgctctaca accgacattc cccattcgtt
     3961 ctcggtcatc ttctgctgaa atggcaacgt gacgccatca aagcgaatca ggcgactgtg
     4021 atgaatgcgc caggcaggaa ttcccgttgc tgtggtcacc acatcgtaaa actcaggttt
     4081 acccaggtcc ggccccatat ctttaatgcg gcgggtcagt gccgggtcga ccatccagcg
     4141 gtcgagcggg agaatcccct taaacttgcc cttaccgatg gtttcgggtc gcagcggggt
     4201 cattggtgcc tgcccctcaa tcatgatgaa acccaccgcg ccgccgtaga ggcgcgacca
     4261 tttcagcacg tcattcagcg catcccagat ttgcaactca tccagttgtg attcgagaat
     4321 gccacgatct tttgcatcaa tttccgaagt gatgcgaatg cctttgcggg tcatatcatc
     4381 cgggatagca tcgactgctt cgccgatgat ccaggatgaa cgataggacc attccaccag
     4441 catgcggtta cgactggtga aattagcccg gtaggtggat gctgagtgct ggttaggtgt
     4501 ctgcatccct actcgg
//
LOCUS       DAJTYL010000194         4513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYL010000194 DAJTYL010000000
VERSION     DAJTYL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.196"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..142)
                     /locus_tag="OO572_003813"
     CDS             complement(<1..142)
                     /locus_tag="OO572_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6662627.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIP"
     gene            complement(156..1214)
                     /gene="yeeE"
                     /locus_tag="OO572_003814"
     CDS             complement(156..1214)
                     /gene="yeeE"
                     /locus_tag="OO572_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6662628.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(1393..2751)
                     /gene="plaP"
                     /locus_tag="OO572_003815"
     CDS             complement(1393..2751)
                     /gene="plaP"
                     /locus_tag="OO572_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6662629.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2741..2803)
                     /gene="yoeI"
                     /locus_tag="OO572_003816"
     CDS             complement(2741..2803)
                     /gene="yoeI"
                     /locus_tag="OO572_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6662630.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(2964..3947)
                     /locus_tag="OO572_003817"
     CDS             complement(2964..3947)
                     /locus_tag="OO572_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6662631.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(3993..>4513)
                     /locus_tag="OO572_003818"
     CDS             complement(3993..>4513)
                     /locus_tag="OO572_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6662632.1"
                     /translation="RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWL
                     HNLPGTSVDILRLAGLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAP
                     KGGHIYNICAPAHPARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFE
                     YQYPDPLVMPLE"
BASE COUNT         1251 a         1202 c         1048 g         1012 t
ORIGIN      
        1 gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg
       61 ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca
      121 catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt
      181 cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc
      241 ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta
      301 ccgatagagc aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg
      361 ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct
      421 gcaataaatg cctccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc
      481 acgtcacccg cgaccagaaa ttgaagaata ttggccgttg gggaagtgac tcctaaccca
      541 aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt
      601 acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccggttcgg
      661 cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag
      721 agcgttatca ccagcaaaac ggtaaccaac ggccacacag acaaattaaa agtctcagca
      781 atagagttat gttcagtagt gtagtgctgc aaggtttgat ttaaaccact ggcgtgtgga
      841 gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat
      901 ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc
      961 ccgaaaagat acccacctat aacagtacca agccacggga acgccccggc ttcgtaagtc
     1021 agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtgccgaaat tgcaatcagc
     1081 aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc accagtcagg
     1141 caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat
     1201 atcattgaaa acataaatta attaaccaga tgaatgttaa tggggaaatt tttcatgact
     1261 ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga
     1321 tttcgataaa aaagaataaa aaaaccggag ccaaatgttc attcgactcc ggtttattat
     1381 tagaaaagat gattatgcta cgtcttcgta ctgcggaacc ggattacgga agctcttggt
     1441 gacgcaagcc aggtaaatca gaccgatagc agcccagatc agacccagaa ccattgagct
     1501 ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag
     1561 atactggaag tgatctttca gcgtcttgtt acgcttctca cggatccaga actgcgagat
     1621 gaccgacagg ttaacaaagg tgaacgccac cagcgcacca aagttaatca gcgccgttgc
     1681 cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat
     1741 cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga acacgccgtc
     1801 acgacccatt acgtacatca gacgcgctac gcctgcgtgc gccgccatac cggacgccag
     1861 tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag
     1921 catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag
     1981 ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat
     2041 cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacagattgc tgatgccgtc
     2101 aaagccggta aaggagaagc acaggattgt cgccccggta atcatcggga taacatgcgc
     2161 atcgccagac cagaacggac gcgtgctcgc cagcgtacca gcgccttcac cttcaaatac
     2221 gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac
     2281 agtgttgacg ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac
     2341 cagcgccacc acaaacatcc acgatggaat ggaaggcacc agagcttcaa aatagatttt
     2401 cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac
     2461 cataaagccg acagtcgggc taatggattt ctgggcgtaa gtgtatgcag agccagcaga
     2521 aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc
     2581 gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaatgtatc
     2641 aaacagcgtc atcggctgca tataggctag acccatcatt acaaccggaa ctaacgtaag
     2701 cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggttattct
     2761 cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag
     2821 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
     2881 tgaatgattg gtttgaagca aaaaataacc gacgctaatt aaacgtcggt ttttaatcat
     2941 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt
     3001 gtcataataa tttttctttg aatggctgat tttccgtcat cagattttgc ataaacaccg
     3061 gcgatcgcac tatttgctaa aatttcatct cgccaccagc cgctggcaag ccctgcggtc
     3121 tgttcattcc agccaatcca gacaggttca aagagagttt gggcgaccac tttcttctcg
     3181 attaacgcgc cactatcgag aaaacgttgc gccagatacc gtggtaaata gccgcagcca
     3241 aggccgctaa tttgcagctc cagtttggtt ttaaaatcga aaacggtaat ggcttcttgt
     3301 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtatcccc caccacaatc
     3361 gcccgatatc gtttaatgat acgacggttt aacggttctt cttcctgggc taacggatga
     3421 tgaggcgcga ccgcaaagac ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg
     3481 gaagatggtg gctcatgcat cgcgccaacg ataatatccg ctcgcccctg agtcagggca
     3541 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
     3601 taaaaagctt caatgagagg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
     3661 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
     3721 gtatgcagaa cttcccgccc tttctctaat aacatttttc cggtgcgggt gaatttagcg
     3781 cggtgtccgc tacgatcaag caattgaata tttaggtcgc tttccagctt gtgaacggtg
     3841 taactcagag cggacggcgt cttgtagagt ttggctgacg ccgccgcaaa actgccctct
     3901 ttttctatgg catcgaggat catcaacaca tccagcagtg gtttcataca cgcccccttg
     3961 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca acggcattac cagcggatcg
     4021 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
     4081 ttgccgctgt ccagactatt tctgaactgc ggaggttgca gccccagtaa acgggccatc
     4141 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
     4201 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
     4261 ttaacaccat gttcaccatc aggggcggtt tttccggcaa agaagcgtcc cggatgacgt
     4321 cccggtccga ccaggcccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
     4381 agccagtctt cgagttcttc taatactcgt ccactgttgg ttaccggatt acgcggggta
     4441 gtttctttaa ccgtgccttg cgcgtcgcca tagacagatg ttgagctggt aaaaataatt
     4501 cggggaatac gat
//
LOCUS       DAJTYL010000195         4511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYL010000195 DAJTYL010000000
VERSION     DAJTYL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.197"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            304..1212
                     /locus_tag="OO572_003819"
     CDS             304..1212
                     /locus_tag="OO572_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6662633.1"
                     /translation="MPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEA
                     TFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKM
                     TNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVR
                     IPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLT
                     QAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGD
                     YLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1212..1946
                     /locus_tag="OO572_003820"
     CDS             1212..1946
                     /locus_tag="OO572_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6662634.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2413..3174
                     /locus_tag="OO572_003821"
     CDS             2413..3174
                     /locus_tag="OO572_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6662635.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3218..4297
                     /locus_tag="OO572_003822"
     CDS             3218..4297
                     /locus_tag="OO572_003822"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6662636.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4294..>4511
                     /locus_tag="OO572_003823"
     CDS             4294..>4511
                     /locus_tag="OO572_003823"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6662637.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYS"
BASE COUNT         1240 a          857 c         1112 g         1302 t
ORIGIN      
        1 attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg cgtagcgttg
       61 tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt gtcgttatac
      121 cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg tttattttgc
      181 gtcatcgtgc tggccgctct cgcggacaga ccccgctgac gcggacgtcc ccttagcggt
      241 ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat ctcaaactat
      301 gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga ccagcgtata
      361 cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg ttgggggtta
      421 ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac aaacatagag
      481 agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga aggtggcatc
      541 gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg tatatgccgc
      601 ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat tgccttaatg
      661 gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc tgttccccat
      721 ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat gaatcaggta
      781 cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat tatgaccgcc
      841 gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag agctgaagct
      901 gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata cctgacgcag
      961 gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg gttcgttcgc
     1021 gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc aatccacaat
     1081 gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga ttatctgaca
     1141 gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg ttttactcgc
     1201 gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca aacatcaact
     1261 gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt ggcctggatc
     1321 tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg aaacttcagg
     1381 ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc gtgatatggg
     1441 taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag gtttgtcgtc
     1501 gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga atgacgaatg
     1561 atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg gatattttat
     1621 tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg tttataaaac
     1681 gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt agcacacgta
     1741 aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag tccgcaattc
     1801 gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt aatgctcaac
     1861 ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa agatgtaaca
     1921 atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct ttttcgatga
     1981 cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt tttgccacgg
     2041 gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag agagttaata
     2101 aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg ttttttaaat
     2161 tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt gtaggctttt
     2221 gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata acaaaattaa
     2281 gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag taaggcgttg
     2341 taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt tgtcgaaatg
     2401 gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg cttgtcgttt
     2461 ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca
     2521 gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca
     2581 gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa
     2641 gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca
     2701 ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa
     2761 ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa
     2821 gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc
     2881 ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag
     2941 ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc
     3001 aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg
     3061 ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg
     3121 attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc ataatgtatt
     3181 acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac tgtcattaat
     3241 tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat ttgattcttc
     3301 acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg gagagcaact
     3361 ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg ttggggagtt
     3421 tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt taaccgttga
     3481 tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg acaataaaaa
     3541 gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg attttgatag
     3601 ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa gtagtttagc
     3661 accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc agacaaatat
     3721 tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt atggcaggtt
     3781 cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat ggagcaagga
     3841 acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag gcataaatgc
     3901 gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt ttgacagcat
     3961 accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg aggacagcta
     4021 ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta ttaaacaaaa
     4081 tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg ataatttacc
     4141 aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg gcgaggagca
     4201 gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat atttcaaata
     4261 tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt ctccgtgggc
     4321 gaaatcacgg cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg gattcaaaac
     4381 gccactcatt ttaatgcaat atctacgggt attggcattt ctctggggct gttagggagt
     4441 ttatcgacag atattacgcg ttcgattgcg aatttatatt atgggaataa atatcgtatt
     4501 cgttatagta a
//
LOCUS       DAJTYL010000196         4505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYL010000196 DAJTYL010000000
VERSION     DAJTYL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.198"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(137..742)
                     /gene="azoR"
                     /locus_tag="OO572_003824"
     CDS             complement(137..742)
                     /gene="azoR"
                     /locus_tag="OO572_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6662638.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            943..>4505
                     /gene="hrpA"
                     /locus_tag="OO572_003825"
     CDS             943..>4505
                     /gene="hrpA"
                     /locus_tag="OO572_003825"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6662639.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKA"
BASE COUNT         1119 a         1143 c         1234 g         1009 t
ORIGIN      
        1 ttatctttat tcgagccaga cgctcgcata ttgtgcttaa ggaagagcac aaagcgatgt
       61 tgaaattatg ggaaaacagg taagtggatt gagatgtgga ctgaatatct acagtccaca
      121 tcaagaccgt gtccggttat gcagcaacaa tgctgtcgat ggctgctttt gcgtcagact
      181 gtgctttcgc tgccatttcc ggaccgtatg cgatcccttc ggcgaagaca aatttcacat
      241 cggtaatgcc gataaagccg aggaacgtgg acagatacgg cgtcaccagg tccgttggtc
      301 catctttgtg gatcccaccg cggctggtaa taacgatggc ttttttaccc gttaccagac
      361 cttccggacc gttctcggta tagcggaaag taacgcctgc gcgtgcaacc aggtcaaaat
      421 agtttttcaa ctgagttgag atgttgaagt tatacatcgg tgccgcaata acgataacgt
      481 cgtgggcttt cagctcggca atcagctcat cggaaagcgc cagagcttcc tgctgacgcg
      541 gagtcagcgg cgcatcgctc ggacgcagag cgccaaccag ttcgccatcc agtaccggaa
      601 tcggatttgc agctaggtcg cgaacggtga tttcatcagc ggagtgcttt tcgctccatt
      661 gttcaacaaa ataatcggac aactgattag actgagagta ccctgccagg atgctggatt
      721 taagaactaa taccttgctc atggtgtttc cttatagatg tttgaatgga cgatgccccg
      781 ttgcttgctg acactttatt cacaatcctg ccacagagat cgcgcaataa atcgaagtct
      841 atgttcgaat ttattgaaca acgcatagaa agccgcgatg tggtactcta tatctatcat
      901 ttaaaagaaa attaatcagg cagactactg cccactaacg ttatgacaga acaacaaaaa
      961 ttgaccttta ctgccttgca acagcggctg gattcgctga tgctgcgcga cagactgcgt
     1021 ttttctcgcc gtctgcattg tgttaagaag gttaaaaatc ctgatgcaca acaggccatt
     1081 ttccaggaga tggcgaaaga gattgaccag gcggcaggga aagtcctgct gcgtgaagcg
     1141 gcacgaccgg aaatcactta tcctgacaat ttaccggtta gtcagaaaaa acaggacata
     1201 ctcgaagcga ttcgtgatca ccaggtggtg atcgtcgccg gggaaaccgg ctccggtaaa
     1261 acgactcagc taccgaaaat ctgtatggag ctggggcgcg gtattaaagg gctgatcggt
     1321 catacccagc cgcgtcgcct ggcggcacga acggtggcca accgtattgc ggaagagctg
     1381 aaaacggagc cgggcggttg catcggttac aaagtgcgtt tcagcgatca cgtaagtgat
     1441 aacacgatgg tcaagctgat gaccgacggg atcctgctgg cggagatcca gcaagaccgc
     1501 ctgctgatgc agtacgacac catcattatt gacgaagcgc acgaacgcag cctgaatatc
     1561 gattttctgc tcggctattt gaaagagttg ctgccgcggc gtcctgacct aaaaatcatt
     1621 atcacttccg cgactatcga cccggaacgc ttttcgcgcc actttaataa tgcgccgatt
     1681 attgaagtct ccggtcggac ctatccggtg gaagtgcgct atcgcccgat tgttgaagaa
     1741 gccgatgaca ccgagcgcga tcagttgcag gcgatttttg acgccgtaga cgaactaagc
     1801 caggaaagcc ccggcgacat tctgatcttt atgagcggcg agcgggaaat tcgcgatacc
     1861 gccgatgcgc tgaacaagct gaacttacgc cataccgaaa tcttgccgct ttatgcgcgg
     1921 ctttcgaaca gcgaacagaa ccgcgtgttc cagtcgcaca gcggacggcg cattgtgctg
     1981 gcgaccaacg tcgcggaaac ctcgctgacc gtaccgggga ttaaatacgt catcgacccc
     2041 ggtacagcgc gtatcagccg ctacagctat cgcaccaaag tgcagcgttt gccgattgaa
     2101 cccatttcgc aggcgtctgc caatcagcgt aaaggccgct gtggtcgtgt gtccgaaggg
     2161 atctgtattc gtctctattc cgaagacgat ttcctctcgc gcccggagtt taccgatccg
     2221 gagattctgc gtaccaacct ggcctcggtt attttgcaga tgaccgcgct ggggctgggc
     2281 gatatcgctg cgttcccgtt tgtcgaagca ccggataaac gcaatatcca ggatggcgtg
     2341 cgtctgctcg aagaactggg cgcgatcacc acggatgaac aggccagcgc ctataaactg
     2401 acgccgctcg gtcgccagct ctcgcagttg cctgtcgacc cacgtctggc gcgtatggtg
     2461 ctggaagcgc aaaaacatgg ctgcgtgcgt gaggcgatga ttatcacgtc cgcgctctcc
     2521 atccaggatc cgcgcgaacg tccgatggac aaacagcagg catcggacga aaaacatcgt
     2581 cgcttccacg acaaagaatc cgacttcctc gcgtttgtaa atctgtggaa ttatcttggc
     2641 gagcagcaaa aggcgctttc ttccaacgcc ttccgtcgcc tgtgtcgtac cgattacctc
     2701 aactatctgc gcgtgcgcga atggcaggat atctacaccc agttgcgcca ggtggtgaaa
     2761 gaacttggca ttccggtcaa cagcgaaccg gcggagtatc gtgaaattca catcgcctta
     2821 ctgaccggtt tgctttccca tatcggcatg aaagatgccg ataaacagga atataccggt
     2881 gcacgtaacg cgcgtttctc catcttcccc ggttctggtt tattcaaaaa gccgccgaaa
     2941 tgggtaatgg tggcggaact ggtagaaacc agccgcctgt gggggcgcat tgctgcgcgt
     3001 atcgacccgg aatgggtaga acccgttgct cagcatttga ttaaacgcac ctacagcgaa
     3061 ccgcactggg aacgggcgca gggcgcggtg atggcaacgg aaaaagtcac tgtttatggt
     3121 ttgccgattg ttgctgcgcg caaggtcaac tacagccaga tcgattcggc gttatgtcgt
     3181 gaactcttta ttcgccacgc tctggtggaa ggtgactggc agacgcgtca cgcattcttc
     3241 cgtgaaaacc tgaaactgcg ggccgaagtg gaagagctgg aacacaaatc acgtcgccgc
     3301 gatattctgg ttgatgacga aacgctgttt gagttctacg accagcgcat cagccacgat
     3361 gtaatctccg ctcgtcactt cgatagctgg tggaaaaaag tcagccgcga aacgcctgat
     3421 ttgctcaact ttgaaaagag catgttgatc aaagaagggg cggaaaaaat cagcaagctg
     3481 gattacccga acttctggca tcagggcaat ctcaagctgc gtttgagata tcagtttgag
     3541 ccaggcgcgg acgcggacgg tgtgaccgta catattccgc tgccgttact taaccaggtt
     3601 gaggaaagcg ggtttgaatg gcagatcccc ggtctgcgcc gcgaattggt gattgctctg
     3661 attaaatcgt tgccgaaacc ggtacgccgt aattttgtgc ctgcaccaaa ctacgccgaa
     3721 gcgtttttag gccgcgtcac accgctggag ttaccgttgc tcgacagcct tgagcgcgag
     3781 ttacggcgga tgaccggcgt taccgttgac cgcgaagact ggcactggga tcaggtgccc
     3841 gatcacctga aaatcacctt ccgcgtggtg gatgacaaaa acaagaagct aaaagaaggg
     3901 cgctcgctac aagatctgaa agatgcgctg aaaggcaaag tgcaggaaac gctgtccgcg
     3961 gtggcggatg acggtatcga gcagagcggc ttacatatct ggagttttgg tcagctgccg
     4021 gaaagttacg aacagaagcg tggcaactat aaagtgaagg cgtggccggc gctggtggat
     4081 gaacgcgaca gcgtgacgat caaactgttt gataacccgc tggagcaaaa acaggcaatg
     4141 tggaatggtc ttcgccgtct actgctgcta aatatttcct cgccgatcaa gtatttacat
     4201 gagaagttac caaacaaagc caagctgggg ctgtacttta acccgtatgg caaagtgctg
     4261 gagctgatcg acgactgtat ctcctgcggt gtggatcaac tgatcgacgc caatggtggc
     4321 ccggtctgga cggaagaagg ctttgctgcg ctgcatgaaa aagtgcgcgc cgaactgaat
     4381 gacacggtgg tggatattgc gaagcaggtc gagcaaatcc tcactgcggt gttcaatatc
     4441 aacaaacgtc tgaaagggcg ggtggatatg accatggcac tggggctttc tgatattaaa
     4501 gcgca
//
LOCUS       DAJTYL010000197         4476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYL010000197 DAJTYL010000000
VERSION     DAJTYL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.199"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..2127)
                     /locus_tag="OO572_003826"
     CDS             complement(<1..2127)
                     /locus_tag="OO572_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6662640.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANW"
     gene            complement(2061..2684)
                     /locus_tag="OO572_003827"
     CDS             complement(2061..2684)
                     /locus_tag="OO572_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6662641.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2681..4369)
                     /locus_tag="OO572_003828"
                     /pseudo
     CDS             complement(2681..4369)
                     /locus_tag="OO572_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
BASE COUNT         1051 a         1217 c         1121 g         1087 t
ORIGIN      
        1 ccagtttgcc ggatgcgaaa gcagcgactg ctgttccacg cgatcgcgtt ccagcgtcaa
       61 taacgcttca tcaattggct ccgggaaagt aatcagcatc ttcgcggttt cgcctggctg
      121 gtacagcgtt ttatccgcca cgatatctac cgtaccagta tgcgccgtgc tgcccttacc
      181 gctgacggca tgacttaacc cagcgagaat taagccgtct ttatcgcgta atgtcagatt
      241 gtagttgcca ggtttagcga aattgacggt aaaggatttg ccgcctgacg gtagctctcc
      301 gctatggctc gtgcggtctt cgagacgcaa ccattcatac gtaacaggaa cctgttttga
      361 agattccagc gcggcataac ggaacacaac cgactcgccg ctattactgt attgtgcggc
      421 ggtacttaaa gagtaatgcg ccaggccgcg ttcaatgagg atctctttgg tggtggtgac
      481 gcgatacgcc gcgccgtcac tggcggagac ggttaacaaa tagcggctcg gtttatcggc
      541 ggcggggaga tttaacgcca catgaccgct ggcgtcggac accgtttcgc tgccttccag
      601 cgacacgggg aaacgtccgg cataacgcaa atcgttaccg accattgata attgctgagc
      661 gcgcaaactt aactgcacgc gggcattttt taccggctcg ccatccgggt agagcagttg
      721 cagtttgccg ctgaccgctt cgccagtttt gaactctttt ttgtcgagag ctaaaccaat
      781 ctcgaaatgt ggcttgatgt agtttgccac gcgaaaactg ctgctataga cctgattgcg
      841 gtaagcaaga cgtaactcat aacctccggc aacggcattt tctggcaggc ggaaacttcc
      901 ctgcccgcca ttgcgcgcat ccagcgtgac atcgacggtt tgcaacagac tgccgttagc
      961 gtccagcacc gaaagcttcg ccggggcgct gacgatgggg gatgaatgca acggatcgcg
     1021 gaactcgcgg cccatcactt taacatcgac acgatcgcct gcgcgatata gcgggcgatc
     1081 ggtaaaaata tacaagcggg tgttgtagat ttcgctttcg tagaagaagt tctcggagac
     1141 aaaaacgccg ccttcagcat ccttacccag aatgtatgaa cgttctggcg atatatgttg
     1201 taactgcaaa gtaccgctgt catcggtcac cccgcgggtc atcacgccaa gaccttcagt
     1261 ccacaagatc tctgagccgg gcttcgcttc accctgtttt ttacccgcgg tccataccag
     1321 aagctcgtta cctgacactt tgctaagcgc cacggtatcg gaaacaaaca ccaccgtcgt
     1381 cgcccgatac ccacctacca tcgcctcgac gaggtacagc cccggctctt gttggccgag
     1441 aggaatataa atattacccg gctgcggcga gatgaaattg ctggatgcgc cttccagttt
     1501 taccccttgc tgcggctcga ccggtttagc ctgccatagt ggataacgga actgttccac
     1561 caagggatat tttttcagcg gggaaaactg gttgttctgt acataacggg aaggtttaat
     1621 aatggcattg ccgagatgta attcgggtaa tgcctgagtc acattctgcc gtgactgaga
     1681 agagaaagta cgctgcatca cgcggcgaga tttgccgtac cagttatccc acagccaggt
     1741 cagcgtattg ttcagcccgt cgcccagata ttgcggttgc accacaatgc gatgcaggtt
     1801 tttctgctgg cgcaaaaatg ccatcgggtc aggaatacga tataggcgaa cgtccacgcc
     1861 gccgtactct tccatctgat agcgccgata atcacgcccc ggcgcttcca gtcgcacttt
     1921 cgcctcttca ctgctgctaa aactgctgtc agcaagcaaa aagaatgttc ccccggcggg
     1981 cggcgcatag ttactggaag gaagcgaatc atcagcttta gcaagccctg ttcccacaag
     2041 agacagggac aagcacgctg ctatcgcgcc agaaaattta aacgataaat gccaatgaaa
     2101 ttgggattgg aatcgttggg tatccatcgg gtgtccttcc atgtcataag ttgttgcaga
     2161 ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa
     2221 cgccccatcc agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca
     2281 ggcagcgcct ggtttatgtc ttggccaata aactggctgt tgtactgaat caaattaatc
     2341 gcggtcacgt aggggccgtt tttcccgctc ccctgattcc agttttgcgc cagttgacgc
     2401 tgttcaggtg ttagcgtcat ctctggcggc aaatactggc tagataaacc gttacttttt
     2461 agccatttac tgtcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca
     2521 caatcctgct gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt
     2581 acaaaccagg cgcgaaacag cctggattgt tcgacattca gcatttcgct atgggcaaca
     2641 acacaacaca gccagcaaat cagcgccagc agcccgtgtc tcatagcggc tgccaggtta
     2701 tgggtagcca ctgccaggcg acaccgggtt ccatctgggc cagcttcatg acataacgcg
     2761 gttgttgaga taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa
     2821 ccggttcgag attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc
     2881 cttgtggatt gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg
     2941 ggcgggtttt attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg agggagaaaa
     3001 gcagcgtttt gttttgcatc gccagcgaca cacggaaaaa ataatccgac attaattgat
     3061 cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacacc
     3121 agatttgcgc ttcgccctgc tgagtctggc ttaccggcaa aacaccttct ggcgctttgc
     3181 tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag
     3241 tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt tgagtcttca taccagcaca
     3301 gccccgcggc accgtctaac gccccattca gcttgtcgtt ttcctggctg atgtgcgaaa
     3361 gcatctcttc ggcaataccg tgtgaatacg gcaccgccac acagaagctg gctccggcag
     3421 gcatactgtt ccagaccggc gtaaaatcga aactggcatc tacgctggcg gattcatcat
     3481 ttaacgccac aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag
     3541 aaggcattaa ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgcaggc
     3601 gtactggcgt tttttcggct gtacgctctt ccaggccaaa gctggcttgc cagcgttttt
     3661 taccgctcaa caaatcactt gcgatcgccg tggcttcggt atcctgctga tcatctttaa
     3721 acaacatatc cgtactggaa aacaccagca tcttgtcctg atatgtcgcg aacatcaggg
     3781 cgttattgcc gttatagcgc aactgataaa caggaatagt ttcactattt atcttgatgc
     3841 tactgatttc cgttttgctt aactagctgt cgctggtagc ggcaaacaac aatggctcca
     3901 gcagtttgct taaaccactg cgctggatca gcaccatgta atgtgaaaga tggccttgtt
     3961 tatcgtgcca tagcgccgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt
     4021 tcagcgtgag atcgtgttca tagacaatgc ggcgaatgct gccttcaatg cccagacgat
     4081 cggcatgatt ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa
     4141 tggtgaggag atctttggga agctggctca gagagtcgct gtctagaaag aggtccggct
     4201 cattgagatc gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc
     4261 cactgccagc atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac
     4321 ctacaagacc atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc
     4381 ttgtgtatag ccagccattt tttacgggca cagccaaact ttaccgcgcc ctaatacgac
     4441 aaaagcccag actttgcagc ctggactttt caattc
//
LOCUS       DAJTYL010000198         4474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYL010000198 DAJTYL010000000
VERSION     DAJTYL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.200"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(51..635)
                     /gene="kptA"
                     /locus_tag="OO572_003829"
     CDS             complement(51..635)
                     /gene="kptA"
                     /locus_tag="OO572_003829"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6662642.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            885..1568
                     /locus_tag="OO572_003830"
     CDS             885..1568
                     /locus_tag="OO572_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6662643.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1565..2026
                     /locus_tag="OO572_003831"
     CDS             1565..2026
                     /locus_tag="OO572_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6662644.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2039..3211
                     /gene="iadA"
                     /locus_tag="OO572_003832"
     CDS             2039..3211
                     /gene="iadA"
                     /locus_tag="OO572_003832"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6662645.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3276..4187
                     /gene="hypT"
                     /locus_tag="OO572_003833"
                     /gene_synonym="qseD"
     CDS             3276..4187
                     /gene="hypT"
                     /locus_tag="OO572_003833"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6662646.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4180..>4474)
                     /gene="iraD"
                     /locus_tag="OO572_003834"
     CDS             complement(4180..>4474)
                     /gene="iraD"
                     /locus_tag="OO572_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6662647.1"
                     /translation="NSPHSALPSLLVSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHE
                     PRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLVWDNGGWRSATLENVS"
BASE COUNT         1026 a         1189 c         1147 g         1112 t
ORIGIN      
        1 cggatgtgct gcgcttacca ggcctatata acccctgcaa tatattgaat ttattgtttt
       61 tgtaccccag tcggcataaa cgaccactca agaaactcaa cagcaaccgt tgatgtcagc
      121 cagaccccgt tttccgcttg ccagaaaggg atgcctcgtt tcgccatttc ctgtgctttg
      181 acggttaaga caaccggcga tccgtgtctt gctcccactt tgcgtgccgt tgcttcatca
      241 gcagagagat gcacgtaatg acgatcccct gcaatcagcc cctgtttttt tatctcgtca
      301 aggaaacggc ttgccgtacc gtggtagaga aactgtggcg gtgttttttc ggcaaaggaa
      361 atagcgacct gcgaagtcga atgcccctga accgcgcgga tgcacctacc atcactggaa
      421 taactaaaac gctttttatc gctggttgcg accacggtat ccagcaatgc acgggttaat
      481 cgcttgcccg ctttttgggc acaaagtatg agcttattga tatccgccca tccttcacgg
      541 tccagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa aaacttgctc
      601 gtatccgcca gctctttttc gttgtacttt gccatcgcgt tttcgtccct ggattttctg
      661 tttttattta ctaatgtcat tgtcactggg tttatgaatt ttctgcaact caatcctgcg
      721 cagaaccttt tccacacagc gtaatttttc ccctatgcaa aaaacgcata gccagaaaca
      781 tacttgcatt ttccccatac ccacatggct gataaggttt ttcagttcca ggaattttcc
      841 tgactcaagc aacacacaaa cacaaaaaaa caatggggat tgttatgaca caacaagggg
      901 atgctgtagc cggggagctg gcgacagaaa aagtgggcat taaagggtat ctggcttttt
      961 ttctcaccat aatctttttt cccggtgttt tttccggaac agacagctgg tggcgcgttt
     1021 ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaat ggagccacca
     1081 cctcatttcg cggggtgggt ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc
     1141 tggcaccgtc agtcattctc tctctgggga ttatttcaat taccgatggt cttggcggat
     1201 tacgcgccgc gcagcagtta atgacacctg tgctaaaacc gttgttgggc attccaggga
     1261 tctgctcgct ggcactgatc gctaacctgc aaaacacgga tgccgccgcc ggtatgacca
     1321 aagagctggc gcaggaaggt gaaattaccg aacgcgataa agtgattttc gccgcatatc
     1381 aaaccagcgg tagcgccatc atcaccaact acttctcttc cggcgtcgcg gtgtttgcct
     1441 ttctgggcac gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag
     1501 ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg caaggagcgc
     1561 aagcatgaca actcaggtcc gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg
     1621 tggctttact atcgccacga ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat
     1681 tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg catatctgtg aacccgtaat
     1741 ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt ttagccgcat taatgagtat
     1801 gggcggcgca gtcggtgttg ccgccagtct ggcaaccgct gacgcattaa ccggccacga
     1861 tgtcaccgtt ttactgcccg caatgtattt aatgggcaac ccggtgcaaa atgtcggtcg
     1921 ctgcctgggg acagccgaag tgaatgccaa atattacccg catatcatta cggtctgcgt
     1981 gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag gagttaccat
     2041 gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt atgcgccgga
     2101 agatcgggga atttgcgatg tcctcgtcgc taacggcaaa attatcgccg ttgccagcaa
     2161 tatcccttct gacattgtac cgaactgcac ggttgtcgat ctcagcgggc agatcctctg
     2221 cccaggtttt attgatcaac acgtccattt gattggcggt ggcggcgaag caggtcccac
     2281 gacgcgcacg ccggaagtgg cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt
     2341 tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg ccaagacccg
     2401 tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc
     2461 ccgcactatt acgggttccg tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt
     2521 gaaatgcgcc atctctgatc accgttctgc cgcaccggac gtttatcacc tggctaatat
     2581 ggcggcagaa tcccgcgttg gcggtttgct cggcggtaaa cctggcgtca ccgtgttcca
     2641 catgggcgac agtaaaaagg cgttaaagcc tatttatgac ctgctggaaa actgcgatgt
     2701 gccgatcagc aagctgctgc cgacccacgt taaccgcaac gtaccgttgt ttgagcaggc
     2761 gctggagttc gcgcgcaaag gcggcaccat cgatatcacc agcagcattg acgaaccggt
     2821 cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg cacgcgtcac
     2881 cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga atttaaccca
     2941 tatcggtgtt gcaggttttg aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta
     3001 tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg gtttccttaa
     3061 catgagcggg aaaggcgaaa ttctgccagg caatgacgca gacttactgg tcatgacgcc
     3121 agaattgcgc attgagcagg tatacgctcg cggcaaactg atggtcaaag acggcaaagc
     3181 ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc agccgcccta atggacgtag
     3241 aatgccccca agggcggctg acagagtaaa acgtaatgga tgactgtggt gcggttttgc
     3301 ataatattga aaccaaatgg ctttatgatt ttctgactct ggaaaaatgc cgcaattttt
     3361 cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc atccgtgcac
     3421 tggaacaggc gattggcgtt gaattgttta accgccaggt gacgccactg caactctcag
     3481 aacaagggaa aatcttccat tcgcagatcc gccatctgtt gcaacagtta gaaagcaacc
     3541 ttgcagagct gcgtggcggc agcgattacg cgcaacgtaa aatcaagata gccgctgcac
     3601 actctctttc cctcggtctg ttaccgtcca ttatcagcca gatgccgccg ctctttacct
     3661 gggcaattga agccattgat gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg
     3721 actgtatttt ttcctttcac gacgaagatt tgctggaagc gccgtttgac cacattcgct
     3781 tatttgaatc tcaattgttc cctgtctgcg ccagtgacga acacggagaa gcacttttta
     3841 acctcgcgca gccacacttt ccgttactga attacagccg caactcctac atggtgcgat
     3901 tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt tttgtctctt
     3961 cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc tggctgccgg
     4021 agtatgccat ccaacaagaa attcgcagcg ggcagctcgt tgtgctgaat cgggacgaac
     4081 aggtgatccc gattcaggcc tacgcatacc ggatgaatac ccgcatgaat ccagttgccg
     4141 aacgcttctg gcgtgaactg cgcgagctgg agattgtgct tagctgacat tctcaagcgt
     4201 cgcactgcgc caaccgccat tatcccagac cagatcaaac gttagcggtt catcgtcgtg
     4261 gtacagcatt atctcaaggc tgatcgccag agtgtggcag tacgcttctt tcaaattaac
     4321 ctgcaacgcc gcaatccgtg gctcatgaaa taacagtgca gaacgcagct gacggcaata
     4381 ccagctctgg agatgtttgt cgctcgggtt atgcacttgc cactcgggca tgcctgaaac
     4441 aagtaatgac ggtaatgccg aatgaggaga atta
//
LOCUS       DAJTYL010000199         4395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYL010000199 DAJTYL010000000
VERSION     DAJTYL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.201"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..497
                     /locus_tag="OO572_003835"
     CDS             <1..497
                     /locus_tag="OO572_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6662648.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO572_003836"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO572_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO572_003837"
     CDS             2500..3114
                     /locus_tag="OO572_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6662649.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO572_003838"
     CDS             3114..3443
                     /locus_tag="OO572_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6662650.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO572_003839"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO572_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6662651.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
BASE COUNT          898 a         1121 c         1194 g         1182 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgaga
//
LOCUS       DAJTYL010000200         4316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYL010000200 DAJTYL010000000
VERSION     DAJTYL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.202"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..3923
                     /gene="pic"
                     /locus_tag="OO572_003840"
     CDS             <1..3923
                     /gene="pic"
                     /locus_tag="OO572_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6662652.1"
                     /translation="YRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSSVSS
                     NGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLVTEV
                     IPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAFKTGGTTGV
                     PLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVAVLRAYAGI
                     NGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLSQGSKNWTM
                     HGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGKTWTGAGII
                     TDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQQADTAGNI
                     QAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQAADYGAVI
                     TNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQTRFYILKSA
                     SYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQLTGNMDVAI
                     PQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLNQKDWENRQ
                     FTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNYVIPEEGTS
                     VPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLTAPGAFAGS
                     SLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYLTDGYDLTG
                     DNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLLEGYNAAFN
                     GAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNKLDATGSDF
                     VLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGTSAEMFKAG
                     TRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYKNFMTEVNN
                     LNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELDGVDLFTGV
                     TMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDYTGNFASLG
                     TKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDMDLSMKNRD
                     FSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGEKDS
                     RMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4074..>4316)
                     /locus_tag="OO572_003841"
     CDS             complement(4074..>4316)
                     /locus_tag="OO572_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6662653.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1157 a          981 c         1168 g         1010 t
ORIGIN      
        1 tttatcgtga tttcgccgaa aacaaagggc tttttgtacc tggtgccaat gatattccgg
       61 tatatgataa ggacggaaaa cttgtgggaa gactgggtaa agccccaatg gccgatttca
      121 gcagtgtgag ctcaaatggc gttgctacgc ttgtatcgcc tcagtatatc gtcagcgtaa
      181 agcataacgg aggatatcgg agtgtgagct ttggtaatgg gaaaaataca tattcccttg
      241 ttgaccgtaa taaccaccct tctattgact tccatgctcc acgtctgaat aaactggtta
      301 cagaagttat tccctcagcg gtaacatcag aaggaaccaa agccaatgct tataaataca
      361 ctgaacgtta caccgctttt tatcgggtgg gtagtggtac gcagtacact aaggacaagg
      421 acggaaattt agttaaggtt gccggtggat atgcttttaa aacaggagga accacaggag
      481 ttcctctgat atctgatgca acaatagtct ctaatcccgg gcaaacttat aatcctgtaa
      541 acggcccttt acctgactat ggagcccctg gggatagtgg ttctcctttg tttgcttatg
      601 ataaacaaca aaaaaaatgg gttattgttg ctgtattaag agcatatgca ggtattaatg
      661 gtgctacgaa ctggtggaat gtcataccaa cagattatct gaaccaggtt atgcaggacg
      721 atttcgatgc ccccgtagac tttgtttccg gactgggccc cctgaactgg acatacgaca
      781 aaacatcagg cacaggtacc ctgagccagg gcagtaaaaa ctggaccatg cacgggcaga
      841 aagacaatga cctcaatgcc ggtaaaaatc tggtattcag cgggcagaat ggtgcaatta
      901 tcctgaaaga cagtgtgact cagggtgccg gttatctcga atttaaagac agttacaccg
      961 tatctgctga atccggaaaa acatggacgg gtgccggcat tattactgac aaggggacga
     1021 atgtaacctg gaaggtcaac ggcgttgccg gtgacaactt gcataagctg ggggaaggaa
     1081 ccctgaccat aaacggaaca ggtgtaaacc cgggaggact gaaaacggga gacggtatcg
     1141 ttgtacttaa ccagcaggca gacactgcag gtaatatcca ggccttcagt tcagtgaacc
     1201 tcgccagcgg acgtccgacc gtggtgctcg gggatgcccg tcaggtcaat ccggataaca
     1261 tttcatgggg ataccgggga ggtaagcttg accttaatgg taatgccgtt accttcaccc
     1321 gactgcaggc tgctgattac ggggcggtga ttacaaataa tgcacagcaa aaatcccagc
     1381 ttttactgga tcttaaggct caggatacaa atgtcagtga accgacgatt ggaaatatat
     1441 ccccctttgg tggtaccggc actccaggaa acctgtacag catgatactc aacagccaga
     1501 cccgcttcta tattctgaaa tctgccagct atggtaacac tctgtggggg aacagcctga
     1561 atgatccggc tcagtgggag tttgttggca tgaacaaaaa caaagcagtt cagacagtaa
     1621 aagataggat cctggccggg cgggcaaaac aacccgttat ctttcatggt cagctgaccg
     1681 ggaatatgga tgtcgccatt ccacaggtgc cggggggaag aaaggtaatc tttgatggta
     1741 gcgtgaacct gccggaaggt accctgagtc aggacagtgg caccctgata ttccagggac
     1801 atccggttat ccatgcctcc atcagtggca gtgcaccggt cagcctgaac cagaaagact
     1861 gggaaaaccg tcagtttaca atgaaaacac tgtcgctgaa agacgctgac ttccatcttt
     1921 cacgtaacgc ctcgctgaac agtgacatta agtcggataa cagccatatc acactgggaa
     1981 gtgacagggc atttgtggat aaaaatgacg gaacaggaaa ttatgtcatt ccggaggaag
     2041 gtacctctgt cccggacacc gtgaatgaca ggagccagta tgaagggaat attacgctga
     2101 accataactc agccctggat atcggcagca ggttcaccgg ggggattgac gcttatgaca
     2161 gtgccgtcag catcacctct ccggacgtcc tgttgacagc cccgggtgct tttgccggca
     2221 gttcactgac agtgcatgat ggcggtcatc ttacagcact gaacggtctt ttcagcgacg
     2281 ggcatattca ggccggtaag aacggcaaaa tcaccctgag cggtacaccg gttaaagata
     2341 cggctaatca gtatgcccct gctgtatatc tgacggacgg atatgacctg accggcgata
     2401 acgcagcact ggaaattacc cgtggagcac atgcttccgg tgatattcat gcctctgcgg
     2461 catcaacagt taccatcggg tctgacacgc cggcagaact ggcttctgcg gaaacggctg
     2521 catcggcgtt tgccggcagt cttcttgagg gctataacgc agcattcaat ggtgccataa
     2581 ccggtggcag ggctgatgtc agtatgcata atgcactgtg gactctgggt ggggactctg
     2641 ccatccacag tcttaccgtc agaaacagcc gtattagttc tgaaggagac cgtacattcc
     2701 gtaccctgac ggtgaataaa ctggatgcaa caggcagtga ttttgttttg cgtacggacc
     2761 tgaaaaatgc cgataaaatt aatgtgactg aaaaagccac tggttcagat aacagcctga
     2821 acgtcagctt tatgaataat cctgctcagg gacaggccct gaatattcct ctggtcacgg
     2881 caccggcggg aacttcagca gagatgttta aggccggcac ccgggtgaca ggtttcagtc
     2941 gggtgacccc aaccctgcat gttgatacca gtggtggcaa tacgaagtgg atactggatg
     3001 gttttaaagc ggaggctgat aaagccgctg ccgcgaaggc tgacagtttc atgaatgccg
     3061 ggtataaaaa cttcatgacg gaagttaaca atctgaacaa acgtatgggt gacctgcgtg
     3121 acacaaacgg tgatgccggt gcctgggcgc gcatcatgag tggtgccggt tctgcagacg
     3181 gtggttacag tgataattac acccatgttc aggtcggctt tgacaaaaaa catgaactgg
     3241 acggtgtgga cctgtttacc ggtgtcacga tgacctatac cgacagcagt gcagacagcc
     3301 atgcattcag cggaaagacg aaatcggtgg ggggcggtct gtatgcttca gcattgtttg
     3361 agtccggtgc ctatatcgat ttgattggta aatatattca ccatgacaat gattacacag
     3421 gtaactttgc tagcctggga acgaaacact acaacaccca ttcctggtat gccggtgctg
     3481 aaacgggtta ccgctatcac ctgacagagg acacgttcat tgagccgcag gctgaactgg
     3541 tttacggcgc cgtgtccggg aaaacattcc gctggaaaga cggtgatatg gacctgagca
     3601 tgaagaacag ggacttcagt ccgctggttg gaagaacagg ggttgaactg ggcaagacct
     3661 tcagtggtaa ggactggagt gtgacggccc gtgccggaac cagctggcag tttgacctgc
     3721 tgaataatgg agagaccgta ctgcgtgatg cgtccgggga gaaacggata aaaggagaga
     3781 aggacagccg gatgctgttt aatgttggta tgaatgcgca gataaaggac aatatgcgct
     3841 ttggtctgga gtttgagaag tcagcctttg gtaaatataa cgtggataat gcggtaaacg
     3901 cgaatttccg gtatatgttc tgatgtgggg gtaaagggct gccggaaggc agcctttctc
     3961 agagactctt aaaatataaa actgaaggtc ttcggtaagg gggagtgtat tagacgtcaa
     4021 ctagttttgc cgactggcgc gagcctgttt aagattctgt gtaaatgcct ttctcagaag
     4081 tgaccgtcca ggcggtcacc gaactcgata ataaagcggc tcattgccat tcgccagtcc
     4141 ttcaacggca tcgtccattt ctgggacgca gactggattg ccagccacac cacttttttc
     4201 accgagtcgt ctgtcgggaa cactttacgc tttttgatcg catggcggat cacgctgttt
     4261 agcgactcga tggcattcgt cgtatagatc actttgcgga tgtccgttgg ataagc
//
LOCUS       DAJTYL010000201         4288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYL010000201 DAJTYL010000000
VERSION     DAJTYL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.203"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            424..1063
                     /locus_tag="OO572_003842"
                     /pseudo
     CDS             424..1063
                     /locus_tag="OO572_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1166..1489
                     /locus_tag="OO572_003843"
     CDS             1166..1489
                     /locus_tag="OO572_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6662654.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1556..3013)
                     /gene="nuoN"
                     /locus_tag="OO572_003844"
     CDS             complement(1556..3013)
                     /gene="nuoN"
                     /locus_tag="OO572_003844"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6662655.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3020..>4288)
                     /gene="nuoM"
                     /locus_tag="OO572_003845"
     CDS             complement(3020..>4288)
                     /gene="nuoM"
                     /locus_tag="OO572_003845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6662656.1"
                     /translation="LLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVF
                     LAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAI
                     LALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDA
                     HSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMT
                     FAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLY
                     ERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVI
                     TVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLG
                     FYPQPILDTSHSAIGNIQQWFVNSVTTTRP"
BASE COUNT         1209 a         1123 c         1051 g          905 t
ORIGIN      
        1 atagagcatc cggcaaaaga gtgagttaac atcattgtta atcaattcga ccagtcagta
       61 aatatgctga ctggtcgaac ataatttgct aaaaagtcat cactgactga tgtcagtccc
      121 accatctgcc agttcaatct acgggtattt ccagcatatc gcggcacgcg attgctggtc
      181 tgttgatgta gtggattgta gttgctgact tttcttaccc tcagatttct gccccacttg
      241 tcaattcaat ctgccaataa ataacataat tatattttta ttcctcacag aaattccttt
      301 ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat acctctacca agcgtaataa
      361 aataaacgct cataattcat tataaataat aatgaaatga cgccttttat tgataattta
      421 catatgaaaa aattttcctt ttgttatatt atttctgttt tcactccctc tgattatttt
      481 taccaaccct gggtcactgc attatcgcca acgccccgac acgtaagtcc tgagcaatta
      541 gaaaaaacag ttcgttatct tacacaaact gtgcatccac gtaatgccgg caatattgat
      601 aatctgaata ggtccgcaga gtatataaaa gagttctttg tcagtagcgg tgccagagtt
      661 accgcgcagg gcgtccccga ttggctatta cgactctgcg cctggaagcc agaattaccc
      721 ttacccggca atgtcctggc tttatcccga tcggggagac tttattgctg tggtcggcag
      781 aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca ttgttgtcat cccgggattt
      841 atctgtttat tctatgaatg ccccagggtt tattcccggt attgattttt ctgatcacct
      901 caattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      961 taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga aaatggctca
     1021 ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat ttctcgaggc
     1081 gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa gatgcgaaac
     1141 ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag aaattgactc
     1201 agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga
     1261 cacgctttca ggattacgcc gactggaatc actggatcga agtgagctgc gtaaacagtt
     1321 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
     1381 agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
     1441 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcagag
     1501 cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta acagtttaca
     1561 tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca cccagcacca
     1621 gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc cagtttgacg
     1681 gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc acgcgcaggt
     1741 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
     1801 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
     1861 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
     1921 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     1981 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     2041 cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     2101 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac gccatcaggt
     2161 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
     2221 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     2281 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     2341 ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg ccgaaacctg
     2401 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     2461 acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa gaagacgctg
     2521 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     2581 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac agagacgcca
     2641 gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2701 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2761 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2821 gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc gccgcgttaa
     2881 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     2941 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     3001 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     3061 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     3121 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     3181 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     3241 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     3301 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     3361 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     3421 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccgcagagaa
     3481 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     3541 ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa accgaggtgt
     3601 aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg tagaagatgc
     3661 cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc gggaacagcg
     3721 gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc gcgaggtcca
     3781 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3841 ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat tccacaccac
     3901 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3961 agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc gcctgggtgt
     4021 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     4081 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     4141 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     4201 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     4261 gcagaccggt cagcacgacc atcagcag
//
LOCUS       DAJTYL010000202         4263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYL010000202 DAJTYL010000000
VERSION     DAJTYL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.204"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(67..1017)
                     /gene="uspE"
                     /locus_tag="OO572_003846"
     CDS             complement(67..1017)
                     /gene="uspE"
                     /locus_tag="OO572_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6662657.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1169..1921)
                     /gene="fnr"
                     /locus_tag="OO572_003847"
     CDS             complement(1169..1921)
                     /gene="fnr"
                     /locus_tag="OO572_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6662658.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2116..2631)
                     /gene="ogt"
                     /locus_tag="OO572_003848"
     CDS             complement(2116..2631)
                     /gene="ogt"
                     /locus_tag="OO572_003848"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6662659.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2642..2785)
                     /locus_tag="OO572_003849"
                     /pseudo
     CDS             complement(2642..2785)
                     /locus_tag="OO572_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3175..3990)
                     /locus_tag="OO572_003850"
     CDS             complement(3175..3990)
                     /locus_tag="OO572_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6662660.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4087..>4263
                     /locus_tag="OO572_003851"
     CDS             4087..>4263
                     /locus_tag="OO572_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6662661.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISA"
BASE COUNT         1108 a         1003 c         1003 g         1149 t
ORIGIN      
        1 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
       61 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      121 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      181 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      241 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      301 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      361 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      421 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      481 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      541 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
      601 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
      661 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
      721 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
      781 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
      841 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
      901 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
      961 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1021 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1081 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1141 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1201 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1261 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1321 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1381 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1441 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1501 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1561 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     1621 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     1681 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     1741 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     1801 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     1861 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     1921 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     1981 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2041 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2101 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2161 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2221 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2281 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2341 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2401 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2461 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2521 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     2581 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     2641 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     2701 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     2761 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     2821 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     2881 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     2941 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3001 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3061 gtgcatcctc gtcttaaatc cggcctgggg tccaggtgac cggtgacagt gttataacgg
     3121 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3181 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3241 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3301 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3361 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3421 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3481 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3541 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     3601 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     3661 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     3721 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     3781 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     3841 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     3901 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     3961 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4021 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4081 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4141 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4201 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4261 gct
//
LOCUS       DAJTYL010000203         4207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYL010000203 DAJTYL010000000
VERSION     DAJTYL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.205"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..355
                     /locus_tag="OO572_003852"
     CDS             <1..355
                     /locus_tag="OO572_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6662662.1"
                     /translation="DSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQ
                     VFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVS
                     AGTVQSVIGVTFIYE"
     gene            689..2968
                     /gene="ydeP"
                     /locus_tag="OO572_003853"
     CDS             689..2968
                     /gene="ydeP"
                     /locus_tag="OO572_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6662663.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3216..3419
                     /gene="safA"
                     /locus_tag="OO572_003854"
     CDS             3216..3419
                     /gene="safA"
                     /locus_tag="OO572_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6662664.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3487..>4207
                     /gene="ydeO"
                     /locus_tag="OO572_003855"
     CDS             3487..>4207
                     /gene="ydeO"
                     /locus_tag="OO572_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6662665.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHT"
BASE COUNT         1189 a          915 c          963 g         1140 t
ORIGIN      
        1 tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag aaatacccct
       61 tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg ccaccactga
      121 cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat cgggagtggg
      181 tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct ccctggggac
      241 agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa caggaaacaa
      301 ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg aataattgtg
      361 attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt attttgtgaa
      421 ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag tcttattttt
      481 tatattggaa aatatgttaa tttaaacact atgagagatg atgcttaact tatcgtattt
      541 aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct tactcttata
      601 tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt acaaatccta
      661 ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct accagggtgc
      721 tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc agatggatat
      781 acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg actgtccggg
      841 ttgtgcatgg ccagatccta agaacagtgc gtcattcgac atttgtgaaa acggcgcaaa
      901 agcaatcgct tgggaagtca cggataagca ggtaaatgcc tctttctttg ctgagaatac
      961 ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc gtctcactca
     1021 gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc aacaagcttt
     1081 cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg aattctatac
     1141 ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc gtgaatacgg
     1201 gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg tgggtttggc
     1261 agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga agtgcgattt
     1321 agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga cttcgttgcg
     1381 cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg aacgtggcct
     1441 ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg agactcagtt
     1501 ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca aggggatgat
     1561 gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct cattgcttga
     1621 tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa
     1681 ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa tcgccgaact
     1741 ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg ggatcactca
     1801 gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga tgaaaggtaa
     1861 cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg tacagggcga
     1921 ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg
     1981 ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca tgcaagcaat
     2041 atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac tggcaatgcc
     2101 agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac acgtagccac
     2161 taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc cggttctggg
     2221 gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat
     2281 gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga
     2341 gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg tagcctggga
     2401 gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc tgccagagtt
     2461 cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa atgcagctgc
     2521 tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca aagggctgtt
     2581 agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac gcagccacga
     2641 tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat tcggtcaacg
     2701 agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag
     2761 agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca tggatagatt
     2821 aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc cagaatcgaa
     2881 tcacatgcta acacttgata accacgatcc attaagtggt attcctggct ataaaagtat
     2941 tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc tgtcatccgc
     3001 acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg attaaattaa
     3061 actacatact ataagatata aacttctgcc tacagctgta agaaactccg ctcagtactg
     3121 aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac tgattaacga
     3181 cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca
     3241 aaatcacgca aagagataac tataaagaaa tcatgtctgt aattgtggtt gtcttattac
     3301 tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt tcagaaatgg
     3361 gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac tggaaataaa
     3421 cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt gttttaagga
     3481 gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa cgctaacatt
     3541 ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa cgctgttcgt
     3601 acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga aaataccatt
     3661 aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc tgcgaagttt
     3721 ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg
     3781 gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt
     3841 gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt ttccgggaaa
     3901 gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac
     3961 tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc
     4021 tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt
     4081 tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg
     4141 ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagg
     4201 cacacgg
//
LOCUS       DAJTYL010000204         4160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYL010000204 DAJTYL010000000
VERSION     DAJTYL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.206"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(31..819)
                     /gene="phoH"
                     /locus_tag="OO572_003856"
     CDS             complement(31..819)
                     /gene="phoH"
                     /locus_tag="OO572_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6662666.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1426..2691)
                     /gene="efeB"
                     /locus_tag="OO572_003857"
     CDS             complement(1426..2691)
                     /gene="efeB"
                     /locus_tag="OO572_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6662667.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2697..3824)
                     /gene="efeO"
                     /locus_tag="OO572_003858"
     CDS             complement(2697..3824)
                     /gene="efeO"
                     /locus_tag="OO572_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6662668.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3882..>4160)
                     /locus_tag="OO572_003859"
     CDS             complement(3882..>4160)
                     /locus_tag="OO572_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6662669.1"
                     /translation="SLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFDMSAVLSTHSL
                     FGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA"
BASE COUNT          955 a         1160 c         1010 g         1035 t
ORIGIN      
        1 gttcgcccgg gctctgcacc gataatacac ttaactgtat gcatgcagcg tacgttggca
       61 aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg atcccaacca tttcatcttc
      121 ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg ccgcgaggca aatcgcattg
      181 cgtgatatca ccgttgacga taaccgtcac gttctccccg aggcgggtta aaaacatttt
      241 catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg acaactgcat tttcaaaggt
      301 acgtccacgc atataggcga acggcgcggt ttccaccttc cctatttccg gtcgcaggca
      361 gtactgcata aaggaagccc ctaagcgccg gaccagcaag tcgtagaccg ggcgaaaata
      421 gggagcaaac ttttctgcga tatcaccagg taagaagcca agatcttcat cggcttgcag
      481 aactggacgg gtgacgataa tcctgtcgac atccttatgt atcagggcct ctgccgcttt
      541 tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc gtggcgaata tcagctgctt
      601 actctcaata gcctgcagat agtgcaattg cgcttcattt cgcgcaagga tgggcgaggt
      661 atcgcgactg tcgcgggcca taccaatggc ttctacgccg ctcatctgca caagcgaggt
      721 gaccgattct tcttcacgct gcttatggct gcgcgaatcc cgtctcagca cacgttttgc
      781 ctcgcgacga gctttgatca ctgctttttg tcttcccatg gagagcacct tgagttgttt
      841 gtattcatca cacgcgccgt tggcagcgcg attatgcgca cgaacatcag agggttggct
      901 tccttgtaag ccatagtttg cttttggata aaatgccgaa aaacggctac gcacaccgtt
      961 tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa aatacagagt gacgaagttg
     1021 ctaccggagg agaaatctcc gcgagtggtg gagcgttgct taacaaaaca ttgtccagaa
     1081 caggacgtta ccatccgcga tctccatagt gactgactat cactgccggg aacttccgct
     1141 gctacttaat aagtacaaca gaactcgcat tcattgcaac aatatattca cttatattta
     1201 acgataacac ccatttcggt gacattaatt tctactggaa agatgacaga gtgatgacag
     1261 tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg ctggtaaaat gtgcctcgtg
     1321 tttgccggat tcggcgcgaa cgccttaccc gacctacaag tgattaataa tttgtgcaca
     1381 aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc gtcgttcaaa cctgcaatag
     1441 cgcccttccc agataatcgt tcgcgtcctt cactcccggc agcgaaaaaa aataaccgcc
     1501 gccgataggt ttaacgtatt cctccagcgc ttcgccattg agcctttttt gtactgtcag
     1561 gaagcctttt tccagatcgt gttggtagca gacaaacagc aaccccatat ccagttgtcc
     1621 ggagttggtg acccccaacg aataactgta gccgcgacgc agcatcaggc tggactcact
     1681 ctccgccgtg cggggattcg ccagccggat atggctgtcc agcgcgatca ccttcccttc
     1741 cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc attcccagcg gcgcaccggt
     1801 ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc ggcgttctgt cccaaaattc
     1861 cactcgaaac tgaatcaagc gtactgcctg atagctgcca ccgattgtcc acgcaggctc
     1921 ctgctgatct gccgttaccc acaccacttt ttgcatcaac ttatcattct ggctatcggg
     1981 attggcagtg ccgtctttga aacccagcaa attaatcggc gtctctttgc ctttactacg
     2041 cgccgcgtga tcggaaataa acccttcccg cttccagcgc acactgagca aatccggcgt
     2101 gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc tgggtgttgg cgcaaatctg
     2161 tagcaacaca tcaccatgac ataacgccgc atccagcgag tcgttgggga aacgcgtcat
     2221 cttctgcagc ttttttggca tctgtggcgc aaggccaaag cgctcatcaa acaatgagtg
     2281 acccaccgat aacgtgatgg tgagattatc gggcgcaatg tagccgccaa gaatgccgga
     2341 atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct gctccgccct gactcagaaa
     2401 agcaaaacgc tgagtcaaca agcgaaacaa ccgctcaaga tcggctttat cgctggcaag
     2461 cacatcaaac gccaccagca tcattgcggc ctgttgtggc gtcaggatcc ctgcctgatg
     2521 ctcaccataa aacggctgtt tctcattgcg tgcatccggt gaaagcgtgc ccggtgcact
     2581 ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc agcgcaccaa tccctttcag
     2641 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     2701 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggccct
     2761 ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     2821 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccaacagt
     2881 tccgggttgg ctttttgcag ttgtggacgc agcagatcga caattttctg cgagccttca
     2941 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctgatt
     3001 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
     3061 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat cggtataaag ctgctcagcg
     3121 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
     3181 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
     3241 ccatccagat cggagaacag ttcagcaatc ggttcaatac gctcatagtg ctggcgcgtc
     3301 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
     3361 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
     3421 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
     3481 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
     3541 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
     3601 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
     3661 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
     3721 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
     3781 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
     3841 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
     3901 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
     3961 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
     4021 gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat cgaaggcgat ttcctgaaag
     4081 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagccgccag ccctgcggcg
     4141 acgaagagaa taaacaggct
//
LOCUS       DAJTYL010000205         4131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYL010000205 DAJTYL010000000
VERSION     DAJTYL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.207"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..515
                     /locus_tag="OO572_003860"
     CDS             <1..515
                     /locus_tag="OO572_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6662670.1"
                     /translation="VLALPFLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGF
                     LDISLDFLFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNP
                     GYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSG
                     YLTLKSLQRQ"
     gene            complement(524..1831)
                     /gene="ynjE"
                     /locus_tag="OO572_003861"
                     /pseudo
     CDS             complement(524..1831)
                     /gene="ynjE"
                     /locus_tag="OO572_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(1898..2551)
                     /locus_tag="OO572_003862"
     CDS             complement(1898..2551)
                     /locus_tag="OO572_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6662671.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2551..4022)
                     /locus_tag="OO572_003863"
                     /pseudo
     CDS             complement(2551..4022)
                     /locus_tag="OO572_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(3995..>4131)
                     /locus_tag="OO572_003864"
     CDS             complement(3995..>4131)
                     /locus_tag="OO572_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6662672.1"
                     /translation="KLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH
                     "
BASE COUNT          948 a         1140 c         1096 g          947 t
ORIGIN      
        1 gcgtgctggc tctgccgttt ctggcgctgg gctggtatct ggcggcgcta gtcgtcattt
       61 tgttgaacag gctgcttgat ggtctggacg gtgcgctggc gcggcgcaga gggcttaccg
      121 acgcgggcgg ctttcttgat atttctctcg attttctctt ttacgcgctg gtgccgtttg
      181 gctttattct tgctgcaccg gagcaaaatg cgctggcagg cggctggttg ttgtttgcgt
      241 ttatcggcac gggcagcagt tttctcgcct ttgctgcact ggcagcaaaa catcagattg
      301 ataaccccgg ttatgcacat aagtcgtttt actatctggg gggattaacc gaaggcacag
      361 aaacgatctt actgtttgtg ctgggatgtt tattccctgc atggtttgcg tggtttgcgt
      421 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
      481 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
      541 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
      601 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
      661 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
      721 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
      781 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
      841 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
      901 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
      961 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
     1021 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
     1081 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
     1141 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
     1201 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
     1261 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
     1321 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
     1381 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
     1441 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
     1501 cggcgtcaac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
     1561 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtccagccag ctagcagaga
     1621 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
     1681 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
     1741 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
     1801 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
     1861 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
     1921 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
     1981 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
     2041 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
     2101 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
     2161 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
     2221 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
     2281 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
     2341 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
     2401 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
     2461 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
     2521 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
     2581 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
     2641 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
     2701 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
     2761 tgaaaatccc accgcgaagg caatcagcac agggcgcaac atgagtgggc atttcacgta
     2821 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
     2881 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
     2941 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
     3001 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
     3061 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
     3121 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
     3181 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
     3241 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
     3301 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
     3361 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
     3421 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
     3481 tcgccagcat cgccattgcc agcggctgta gcccagcgaa tccagcacaa tgacctgctg
     3541 caacagccgt ttttcgctca atactgccac taagatccac agcagaaatg cgctttcttt
     3601 cactgcaagg gtgaggccca gaccgatcca aatcggtcca ttggcggagt gaaatacggg
     3661 aaatagtcat atagcagccc tccgtcagca aagagcagca gggcgctggt ggcaaaagcc
     3721 acatggggaa tggcgagcag ccacggcaga cgggcgcaca tacgctgcca tttcggccca
     3781 ggccatagcg ccacaatcac cagcagggca atcaacaatg ccccgaccgc cgcgatggtt
     3841 gtcgacacca gcgttgccag taatgcctgc ggtaactgtg gatcggcaaa taacgcctgc
     3901 cagcgtgtca acgacaacgc aggcgatatc agcgtgagag ctgccgggat cagcggtgca
     3961 taaatcaccg ccaccatcgc ccacagcaaa aagattaatg cgtaccgtaa cggcgtaacc
     4021 attcttgttc cagtgcattc acccatcctg cgtgcggttc agccagtacc ggcggcagat
     4081 cctgcggcat tcttgattgc aatatttcgc gctgcccgtc aggcagtttt t
//
LOCUS       DAJTYL010000206         4052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTYL010000206 DAJTYL010000000
VERSION     DAJTYL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.208"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(896..1087)
                     /locus_tag="OO572_003865"
     CDS             complement(896..1087)
                     /locus_tag="OO572_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6662673.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1152..1475
                     /locus_tag="OO572_003866"
     CDS             1152..1475
                     /locus_tag="OO572_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6662674.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1417..2175
                     /locus_tag="OO572_003867"
                     /pseudo
     CDS             1417..2175
                     /locus_tag="OO572_003867"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2153..2683
                     /locus_tag="OO572_003868"
     CDS             2153..2683
                     /locus_tag="OO572_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6662675.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2690..2923
                     /locus_tag="OO572_003869"
     CDS             2690..2923
                     /locus_tag="OO572_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6662676.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3469..3861)
                     /locus_tag="OO572_003870"
     CDS             complement(3469..3861)
                     /locus_tag="OO572_003870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662677.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          969 a          922 c         1208 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctaacac ccgctgacaa gcccgtcaga
      901 ggttttcacc gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt
      961 cgtaaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca
     1021 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1081 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1141 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1201 gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg
     1261 cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     1321 ggaataacct gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
     1381 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     1441 ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1501 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
     1561 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     1621 tacacgtccg gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa
     1681 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt
     1741 ctgtgggtgg agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg
     1801 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt
     1861 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1921 aaccgccgga cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag
     1981 agcattacac ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
     2041 gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc
     2101 agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa
     2161 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     2221 gaaaatgctg agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc
     2281 aaacgagacg aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact
     2341 gaaaaaacat cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa
     2401 gctggcaggg aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag
     2461 tggcgggacg ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa
     2521 gcagaacgag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg
     2581 gagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat
     2641 ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct
     2701 ggaaagccat ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa
     2761 cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa
     2821 cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca
     2881 gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga acggttgata
     2941 caggagcgcg aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg
     3001 acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg
     3061 tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc
     3121 agaccttgct gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc
     3181 ttttgtcgct gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt
     3241 tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg
     3301 tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa
     3361 tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     3421 tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt
     3481 gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga
     3541 ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa
     3601 ggcgtgagat tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct
     3661 tccgtccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac
     3721 ttcttgaatt tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt
     3781 aggggagtgt attattctct cttttaataa gctgtttacc acagggttta gttgttgtct
     3841 aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg
     3901 ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc
     3961 aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct
     4021 tttttgcggc gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTYL010000207         4029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYL010000207 DAJTYL010000000
VERSION     DAJTYL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.209"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..74)
                     /gene="rcsF"
                     /locus_tag="OO572_003871"
     CDS             complement(<1..74)
                     /gene="rcsF"
                     /locus_tag="OO572_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6662678.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPV"
     gene            complement(192..1007)
                     /gene="metQ"
                     /locus_tag="OO572_003872"
     CDS             complement(192..1007)
                     /gene="metQ"
                     /locus_tag="OO572_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6662679.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(1047..1700)
                     /gene="metI"
                     /locus_tag="OO572_003873"
     CDS             complement(1047..1700)
                     /gene="metI"
                     /locus_tag="OO572_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6662680.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(1693..2724)
                     /gene="metN"
                     /locus_tag="OO572_003874"
     CDS             complement(1693..2724)
                     /gene="metN"
                     /locus_tag="OO572_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6662681.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            2912..3487
                     /gene="gmhB"
                     /locus_tag="OO572_003875"
     CDS             2912..3487
                     /gene="gmhB"
                     /locus_tag="OO572_003875"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6662682.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            3851..>4029
                     /locus_tag="OO572_003876"
     rRNA            3851..>4029
                     /locus_tag="OO572_003876"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1067 a          989 c          987 g          986 t
ORIGIN      
        1 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
       61 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
      121 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
      181 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
      241 taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
      301 tcttcacgcg ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg
      361 tctttcgccg gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg
      421 atttgcgcgt cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga
      481 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
      541 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt
      601 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
      661 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
      721 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
      781 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
      841 ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg
      901 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
      961 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
     1021 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
     1081 cctgcaaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
     1141 gtcgcgttgt agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
     1201 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
     1261 accagacccg gcaacgcttc cggtaacagc actttacaga cgatctgcat tggcgtggca
     1321 cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
     1381 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca
     1441 atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
     1501 gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
     1561 ataatttgcc ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac
     1621 ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc
     1681 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
     1741 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
     1801 tttaacgcca ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
     1861 acgcgcggtt tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag
     1921 catcggcacg cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat
     1981 atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa
     2041 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc
     2101 acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
     2161 gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct
     2221 ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg caatcgccac
     2281 acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc
     2341 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag
     2401 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
     2461 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa
     2521 ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaagt ttacacaacg
     2581 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
     2641 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
     2701 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca
     2761 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
     2821 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
     2881 cgtcccatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct
     2941 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
     3001 atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt
     3061 ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
     3121 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
     3181 ttattgcccg catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg
     3241 caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
     3301 ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg
     3361 aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga
     3421 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
     3481 gcaatgatta aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag
     3541 ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag
     3601 ccggttcggt tgaagagaaa aaatcctgaa attcagggtt gactctgaaa taggaaagtg
     3661 tgatatacgc cacctcgcga cagtgagctg aaagctgcgt cgcaactgct ctttaacaat
     3721 ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc aagacgataa
     3781 atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat tctttgagca
     3841 tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta
     3901 acacatgcaa gtcgaacggt aacaggaagc gcttgctgct tcgctgacga gtggcggacg
     3961 ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta
     4021 ataccgcat
//
LOCUS       DAJTYL010000208         4007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYL010000208 DAJTYL010000000
VERSION     DAJTYL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.210"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..236)
                     /locus_tag="OO572_003877"
     CDS             complement(<1..236)
                     /locus_tag="OO572_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6662683.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQA"
     gene            complement(246..938)
                     /locus_tag="OO572_003878"
     CDS             complement(246..938)
                     /locus_tag="OO572_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6662684.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(941..1396)
                     /locus_tag="OO572_003879"
     CDS             complement(941..1396)
                     /locus_tag="OO572_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6662685.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(1396..2244)
                     /locus_tag="OO572_003880"
     CDS             complement(1396..2244)
                     /locus_tag="OO572_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662686.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(2244..3662)
                     /locus_tag="OO572_003881"
     CDS             complement(2244..3662)
                     /locus_tag="OO572_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6662687.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            complement(3662..>4007)
                     /locus_tag="OO572_003882"
     CDS             complement(3662..>4007)
                     /locus_tag="OO572_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6662688.1"
                     /translation="SPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPD
                     YALEASAKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNY
                     FHRKEPCDNGSE"
BASE COUNT          947 a         1042 c          983 g         1035 t
ORIGIN      
        1 gcctgctgaa attctttctg ccgctgagcc tgcttttcct gctgaaaaac acccgcaata
       61 cctgaaagac cttgcaaagc agtcagccca acattgttag cgcctgaacg ctcaatatca
      121 ttgttctgcc tgataagctg aagcgtattg ccgatgtcat ttacgctcgg agcgtttgag
      181 ttgacgccgc cgataccagc caacaatccg ccgtttgttc cttgccaagt agccatgatt
      241 cccccttaaa acaacgagcc aagcaatccg ataccagcac caataccagc gccccaaggc
      301 gttgatgttc ccaaaaggct ggcaagacct gcaccggcaa tcgcaccaga cgtgccaccg
      361 ctaattgcag tctgaagact tgatggttta ttggcattag cagcggcaag agctgcgctt
      421 tgctgtgcaa tgctgctcat gttgttggcg tacgtctgcc cggcgtttgc ctgaccttgc
      481 agcgcaccaa gcccaacgtt tgccagattg ttgtaattgc tcatctgatt tgataaccaa
      541 gactgaccga gtgtcggcgc gatcgtagcc agttgattgc ttgtggctgt cgaaccaagt
      601 ccacccgtcg cctccgcagc agcaagactc tggtaacgcg cctgccctgc aaggtcttta
      661 tactgcttag agttgtaata ctgattaagt gcctgcccct gaccttctaa actggaaagg
      721 ttctgaagct ggttaacata ctgctccgca agaggcgtga acggagcaag gtttttcatg
      781 atcgtctgcc actgctgatt ttgcaggtct gcagcatact tctgggcttc tgctgcatac
      841 tttgcgcttt tatcagaact gccacctttc ccgccttttt cagggcaata aggttcctcg
      901 ccgcgcagtt ttctgcccag cttaaatgca tataacatgg ctatctcccg tgattcagga
      961 agtcgattag ttcttcgcgt gtggcgctgt aaaaagtcac gtcatccacg cctttgaagt
     1021 atttcttgat ggttcctaca cgcttaaggc caatcattgc gcagtacatc tgaccgtgtc
     1081 ggaatttgcg tgcagcgaac gatgttacgc actgaacggt ggtgttagtc agaatgtatc
     1141 gccagaacgc cagcccaatt tccttgctga agccgcgaat ctctggcagg tacatggcgt
     1201 ggcaatctaa tgtcagcggc tgaatctcct gatagtaaac aatgccgcca aactgaccgt
     1261 gcacgttcac ctcaaagtaa cggcattcag gtttgtagtc gtatccatca ccgttgttgc
     1321 tcccggcgat aatgtcaggg tgatttccga ctgcttctat caggtcgatg tttcgcgttg
     1381 gtttgaatgt aatcatcact gctccgcgat tatcttgatg gttgtggcag taaacgccgc
     1441 accattcgac tgaatggtta acgtactgcc atttgtggca agaaagccgt ctttatccac
     1501 gctgaagaac gtagctaaca ggatgttgtc ggttgttgtc gccgcattac gactgctgac
     1561 cagtgtgtca ggaacagagc cggaaaaggt tagctgcatt gacctgttgg cggttccgct
     1621 gggccacgtg ccgacaatcg acaacttgaa gagcagggtt ttgttctcgt tgaacacaac
     1681 catcttgttg ttaacggtgt cgaagaatgg tgccaacgag ccggatgacg gtgtgagcgt
     1741 tttcagcagg ctaacaaggt tggtcggcgc tgtcgggatg gttacagata caccagagta
     1801 aacaacctct gacttcttgc gtgtggttgc atactccaga gcatcgatgc gcgtttcatg
     1861 gtctgaaacc tgcgactcca gcgactgaac tcttgtgtca agcgacgaaa tatcgctttc
     1921 attctgagct attcgtgttt catggtcctg aagagttgat tctgcctgtc tgattcgctc
     1981 ctcatgatta acaagcgttg cttccgcagc agaaattcgc tgctcatggt cagcgagaat
     2041 cacatcctgc tcatcgttcc tgacctgtgc atcataagcg ccctgtccgg cctcgttggc
     2101 tttgttagcc acgttaccaa catcagtgcc ctgtgcgata acgtaaagca gatacgactg
     2161 cgagaagata ttgcgtggaa ggactgatgt atcgagccgc gtagcttgga tgattaccgg
     2221 cacattgaga ttcgaatcag ccattactca atccttatct gagcgccaga cagagtgaca
     2281 ggtgacttcg tgataacgcg caatttgaag ccgacatttt tcctgatgcg ccctactcgc
     2341 ttccacaaaa cacgcttgtc gtaaacgaac ggttcattct gctcaatcat ctgctcacgc
     2401 ccgtaattga tgcagtcagt ggttgcagag agaaaaaggc ggtcggcgta ctgcgcaacg
     2461 ccagttgacg attcaacctc aaggtcgaac actctggcgt tatccgcttt gaagagtgga
     2521 gtaaacagca ggtgttcctg ttgcttgtcg tactggctgc tgatatcgaa ttgcagtttc
     2581 ccggtaacag attccagctt atcgccgcac gttatctgat tgccttcgta aacgaagtcg
     2641 atagcgcggt acacatcgtc atacagacct gttttcagca cacaccattg tgggccattg
     2701 gcgattgaag atgcgtcgta cacgaggacg tgacgcggaa ggtggataat cagcaactca
     2761 tgagcatcaa atcgcagcga ttccatcaca ccatcagcca gttcatcagc agtgtaggag
     2821 cggaggattt tctcaatgct cgcgctggca attggtgaca cctgaccgga gtcgatgata
     2881 tacacagacg gcgcacccgt tgccggattg ctgataaacg catacgaatc agcgaatggc
     2941 gttttgcagt aagttccggc aatgcctttc tgcaccatca gcgatggttg tgcaacatac
     3001 aaagcggcac caacggtggt tgcgccagtc agggagaaat attcaatagt cgatgaacca
     3061 aagcagacga tgaagtctcg ccatgtcccg ataccgatga tgccgtcagg ctgagactcg
     3121 gcacgatatt gtgcgctgta acggtcagga tgagattcgt cttcaaggtc agtgataaac
     3181 catgaatcag ttccgtcttt tgaccacgca taacgcccac gtaagcgcgt aatgtcgcga
     3241 actgaaccta actcatactg agtgaatcca ctgtctgtag gccagtttga gacggttttg
     3301 accgtgccat catagcgata ctcgaccagt tgaccattaa cgcctaccgc ctgtgatgtc
     3361 cgaccatgcg ccattgatac acgaccactt ccggcaacat caccgacttc actttctcct
     3421 ttgtacagct tgccgccaca cacgcgataa acagcattct gcgccatgtt gtactcgacg
     3481 ccgcgagata caccgttcac gtcagaacgt ttggcaatgc ccgggaatga gcgaagatat
     3541 ccgctgctgt tcaggatttc tttgggtgta gccagcatat tcactggcag atagtcgata
     3601 tagtcggcgt ttcggaagtc tttgccgaca cctttcataa gcggaagttg ctgaataggc
     3661 attattcgct cccgttatcg caaggttcct ttcggtggaa gtaattccaa ccattccact
     3721 tcgccaactg gttaccacta ccaacaggca tacggtttgg ataaccggac ttacatttag
     3781 ctgcttttgc cctgtccatt gcagacagtt tgacgagtcg ctctttcccg tatctggcag
     3841 tggttataag ttttgcagac gcttccagcg cataatctgg agcaattcgg caggcaaggt
     3901 tgaaaatgac ggcattgata gcgttatttg ataaaccgtg ctcatcgccc ggatccggag
     3961 caacatctgc atcagcaaaa atgtagccaa cgttgatacc aggtgac
//
LOCUS       DAJTYL010000209         3999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYL010000209 DAJTYL010000000
VERSION     DAJTYL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.211"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(225..1082)
                     /gene="sitD"
                     /locus_tag="OO572_003883"
     CDS             complement(225..1082)
                     /gene="sitD"
                     /locus_tag="OO572_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6662689.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1079..1936)
                     /gene="sitC"
                     /locus_tag="OO572_003884"
     CDS             complement(1079..1936)
                     /gene="sitC"
                     /locus_tag="OO572_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6662690.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1933..2760)
                     /gene="sitB"
                     /locus_tag="OO572_003885"
     CDS             complement(1933..2760)
                     /gene="sitB"
                     /locus_tag="OO572_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6662691.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2760..3674)
                     /gene="sitA"
                     /locus_tag="OO572_003886"
     CDS             complement(2760..3674)
                     /gene="sitA"
                     /locus_tag="OO572_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6662692.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1087 a          981 c          964 g          967 t
ORIGIN      
        1 gttaggaatt ttatcgtgga ataacagaag ctgcggcacg tcgtatgcag gaacgtgccg
       61 cggttggctg gtaaactttc gatagtgcga gtattgaatg atttctaggc gttatcgatt
      121 ttacgtattt tttgcatgat agaattcgta cctcctccca acgaccttcc atggctttcc
      181 ggacttctgt aggctggcgg acgatgctgt cctcggatgt tgtgctaatt gggtgataac
      241 agatcctgtg tatgggcgtt tccctttgtg cgtgcgttga tgctggtaac ggtaaacgtc
      301 atgatgaaca cgaccgcgaa taagacgaca atcgtcggag ctggtgcgct gtcgataaaa
      361 aaggacagcc agacaccagt cattgacact attaccgata tgccagtcgc cagtaggagc
      421 gcaatatgaa agcgttgtgt caggagtact gcaatggcgc caggcgcaat gagcagagaa
      481 atagagagaa tgatacctac tgctttcagc gtcgccacaa tggtcaggga aaccatgcac
      541 agcagcccat aatgcagcca tctcgtatgc aaaccgctta cctgcgcctg ttgataatca
      601 aagctgaaca gcaaaaaatc ccgccatttt acgctaataa caagtgtaac cagcccggca
      661 ataatcaccg tctggattat gtcgccgatg gttatcccga gcatgtcgcc gaaaagaata
      721 tggtcaagat gtacgtctgg tttaattgca atatacaaga tcagacctgc ggcaaacatg
      781 ccagaaaaaa caatccccat aactgtatct tgcttgattc ggctgttgtc tttcaggtat
      841 cccgtcgcca ccgcgcagaa tactccggcg acgaatgccc ctgtagccag aggtaacccc
      901 agaatccagg taagcacgat gccaggaaaa accgcatgac tcatcgcgtc acccatcagt
      961 gcccaacctt tcaggaccag aaagaccgac agcagcgcac agggtatgga aactaccagt
     1021 gaaatcagca gtgcgtgact cataaaggtg aactgcagtg gctccagtaa taatgccatc
     1081 atgagcgatc ctccagcgct ttatgcgcac gccgacgatt tgccaaaagt ccatgggtgg
     1141 gcgcaaatac aaatgctaaa agaaacagca gtgtctgcgc caccacgatg attccacctg
     1201 tcgcgccgtc cagaaagtaa ctgacccacg cgccgaggaa actggttaca ctgccaatag
     1261 ttacagcaat tatgagcagg cggggaaaac gatcggttaa caaccatgct gttgcaccgg
     1321 gagtcaccac cagacagatc accagaaagg caccaaccgt ttgcagcgcc gcaacggtcg
     1381 aaaccgacag cagggtgaag aagaggattt ttaatctgcc cggatgtaat ccaatagcac
     1441 gggcgtgatt ctcgtcgaaa aaggtcacca taagatcttt ccatttgaaa aaaagcacga
     1501 tgatcgagag aatgccaatg atagtgagtt gcaggatatc cgccggatcg attgccagaa
     1561 tattgcccag gacgatggtc tgaatgttca cggatgtcgg gttcaatgac accataaaca
     1621 gccccaggcc aaaaaaggac gagaagatca ggccgatgat ggcatcttct tttaagcggg
     1681 tgcgttggtt aagaaacagc atgctgcccg ctgccagtcc gccggaaaag aaggctccca
     1741 gagaaaacgg aagccccagc atatatgccc ccgcaacacc cgggacaata gagtgcgaca
     1801 gcgcatcgcc aatcagcgac cagcctttta gcatcagata gcatgacagg aaagcgcaga
     1861 gaccgccgac cattgccgag acccacatcg cattgagcat atattcatag ctgaagggtt
     1921 ccagtagcac gttcatcttg cttccctctg caccgctgac ggtcgatgtg ccacaaaagg
     1981 gcgttcatcg tcagtaataa tgctttcttc cgagccgtta agtgtgacat ggcgcagtac
     2041 gccgctaaaa gccagctcca ggttctcggc tgtaaaagtg gtgtctgtcg gcccgcttgc
     2101 caggacggtg cctttgacca tgaccgtata atcgcaaaat gtcgtgactg agccgagatt
     2161 gtgcgttgag acaagcatcg ttttgccttc cgcgcgtaac tccctcaaca ggctgatgat
     2221 tttcgcttcg gttttcacat caacgccggt aaaaggttca tccagcagga tcacatcacc
     2281 ctgctgtgca attgctctcg ccagaaagac gcgtttcttt tgtccaccgg aaagctcgcc
     2341 gatttgacga tggcgaaaat ccaccatatc gacacgttcc agcgcatcgg tgacaatctg
     2401 acgatccctc tttttggcta tacgcaacat acccatatgc ccatagcggc ccatcatcac
     2461 cacatcttcc accagtacag gaaatgacca gtcaacctct tctgactgag ggacgtaggc
     2521 aaccaggttt ttctgtagcg cctgtcgcgt gggaataccc agaacagata tctttccgct
     2581 ggtcagacgc acaaatccca taatcgcttt aaacaacgtc gattttccgg aaccgtttac
     2641 cccaaccaga gcggcaatag atccacttgg tactgtgaag gatgcatcgc gcagtgctgt
     2701 gtgcccgtta cgccaggtga cagtaacatc attaacgaca atgcctgcag attgcatcat
     2761 tatttctccc ttttcccggc tttaattccc tgtaccagcg tactggtagt aaccttaaga
     2821 aggtcgatat acgtaggtac cgggccgttt tctgtgctca gggaatcgac atagagcaca
     2881 ccgccgtagt gtgcgccggt ttcacgcgca acctgacgcg ctggtttatc ggaaatcgta
     2941 ctctcgctaa agactgccgg gatatgattt ttcttaacta tatcaacaac cttacgtacc
     3001 tgctgcggtg ttccttgttg atcggcatta atcggccaca gataaagctc ttttagcccc
     3061 aaatcgcgtg cgagataaga aaaagcccct tcactggtga ccatccatcg ctgattctca
     3121 gggagttccg taatctgctt acgcaggggg gcaagggttt gggtaatctt ggctttataa
     3181 gtatcggcat tacgttggta ggtttgtgca tttgccggat cgtattttat caacgcatca
     3241 cgaatattat cgacgtaaat cagagcatta tctggcgaca tccatgcatg gggattaggt
     3301 ttgccctcat agggtccttc ggtgatccct actggcgtca cacccgaaga gacaattact
     3361 tctggaaccc cattgagatg ctggtaaaag cgttggaacc acaattccag attcatacca
     3421 ttggcgagaa tcagttgtgc cccctgcgca cgtttaatat cgccaggggt aggctgatac
     3481 tcatgaattt ctgcaccagg cttggttatg gatgagactt ctgcagcatc tccagccacg
     3541 tttttggcca tatccgcgat gatggtaaat gttgtaatga ccttgaattt ttcagttgca
     3601 cttgcctgaa atgcgatcga gcaggtgagg gcgaggcacc ccaagagcat ggttactttt
     3661 tttattgagt gcataattta gtacctatag tgagcaaaat tagaacaaac atagcaacgg
     3721 ctatgtttta tagcataacc tatacaaaat gaaaataatt atcattaaca gctttgaggc
     3781 ctaaaacagg gattaggttg tttcctgctg gcgacatgat attgaggaaa aaagtagagc
     3841 agctattagg ctttgaaatg actttatgag gaaaattgtt gatggcaaaa atggggcaaa
     3901 aaagagtggg ttatcgtagc ttattgttgt cgctgataat gtttaacata ttgaaaaata
     3961 aataaaatac ttatgagtca gatagttgtg atttttgcc
//
LOCUS       DAJTYL010000210         3949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYL010000210 DAJTYL010000000
VERSION     DAJTYL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.212"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..171)
                     /locus_tag="OO572_003887"
     CDS             complement(<1..171)
                     /locus_tag="OO572_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCR6662693.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTE"
     gene            complement(176..361)
                     /locus_tag="OO572_003888"
     CDS             complement(176..361)
                     /locus_tag="OO572_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6662694.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(358..2997)
                     /locus_tag="OO572_003889"
     CDS             complement(358..2997)
                     /locus_tag="OO572_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6662695.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3205..>3949
                     /gene="ldhA"
                     /locus_tag="OO572_003890"
     CDS             3205..>3949
                     /gene="ldhA"
                     /locus_tag="OO572_003890"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6662696.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALK"
BASE COUNT          985 a         1049 c          925 g          990 t
ORIGIN      
        1 ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat aaccataaaa
       61 tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca ttacattgct
      121 gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca tcgcctcaga
      181 aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg atgatgatct
      241 catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca gcgacttcaa
      301 tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt aaaattttca
      361 ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac gtgcgttctg
      421 ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt
      481 ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc gactggtacc
      541 tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt ttgtcgatga
      601 ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca tattgtcttt
      661 caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc tatttgccag
      721 ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg caccactaaa
      781 tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc tggatgagag
      841 attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagcac
      901 gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt cttcagtcca
      961 cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc
     1021 ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg
     1081 gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc
     1141 tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac
     1201 ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg
     1261 gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa taccgtccca
     1321 gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa
     1381 ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc
     1441 gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg
     1501 tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat
     1561 cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa tcgtgctgtc
     1621 atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactcccttt
     1681 tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg ccagcccatc
     1741 catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg
     1801 tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat
     1861 gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct caaaggtcag
     1921 cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc
     1981 cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca tgcttaattt
     2041 tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg tcagcacatt
     2101 cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga ctttgacacc
     2161 cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat cgcttacggt
     2221 caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc
     2281 cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgacgg
     2341 ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc catccggcac
     2401 gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa acgcgacgac
     2461 atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc
     2521 gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca
     2581 ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag gcagcatgga
     2641 ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc
     2701 cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga
     2761 aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg
     2821 aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag
     2881 cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa
     2941 cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg
     3001 tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt
     3061 aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat
     3121 cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga
     3181 ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac
     3241 aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt
     3301 ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta
     3361 aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc
     3421 gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga actggggctg
     3481 aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc catcggtatg
     3541 atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc
     3601 tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc
     3661 ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg
     3721 ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt cgatctgcca
     3781 accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac
     3841 catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc
     3901 agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaa
//
LOCUS       DAJTYL010000211         3940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYL010000211 DAJTYL010000000
VERSION     DAJTYL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.213"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            107..829
                     /locus_tag="OO572_003891"
     CDS             107..829
                     /locus_tag="OO572_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6662697.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(957..3940)
                     /gene="yfaL"
                     /locus_tag="OO572_003892"
                     /pseudo
     CDS             complement(957..3940)
                     /gene="yfaL"
                     /locus_tag="OO572_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT          903 a         1134 c          976 g          927 t
ORIGIN      
        1 gcaaattata acacagccgc gcagtttgag gtaaacctat acgctttatt cacatccaat
       61 gcctgatata ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga atgccgaaaa
      121 atcgccggaa aaccataacg tagaccacga agagatcgct aaatttgaag ccgtcgcctc
      181 ccgctggtgg gatcttgaag gtgagttcaa accgttgcac cgcattaacc cgctgcgtct
      241 gggctatatt gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg atgtcggttg
      301 tggcggcggc attctggccg agagtatggc gcgcgaaggc gcgacggtga ccggtctgga
      361 tatgggcttt gagccattgc aggtggcaaa gctgcacgcg ctggaaagcg gcattcaggt
      421 ggattacgtg caggaaaccg tggaagagca cgcggcaaaa catgccgggc agtatgatgt
      481 ggtgacctgc atggagatgc tggagcacgt acccgatccg cagtcagtgg tcagagcctg
      541 tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg acgcttaacc gcaacggcaa
      601 gtcatggctg atggcggtgg ttggcgcgga atatattttg cgcatggtgc ccaaaggtac
      661 gcatgatgtg aagaagttta ttaaaccggc agagctgctt ggctgggtgg atcagaccag
      721 tttaaaagag cgacatataa ccgggctaca ttacaacccg atcactaatt cctttaagct
      781 cggccccggc gtggatgtga actatatgct gcacacgcag aataagtgag gttgatgttt
      841 ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat caggcctaca aatgctctcc
      901 gtaggccaga taaggcgttt acgccgcatc cggcaattgt gatggtcaga cagcgattac
      961 catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact ccctttctgc
     1021 cacgccacgc tgccgcgcag ggacactcgc tgactgatat tgcccgtgac tcccactttt
     1081 atttcacccc gtagcttcac cgcatcgtca ctgatagtgc tgccatcttc ttcaatttcc
     1141 gtcgcatggg gatcgtgata gtaattcaga tctaatgttg gtgtgacacc aaccgcggta
     1201 cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat ccccctgcga ttgtgacacg
     1261 cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc
     1321 tgcggttcaa tcaccacacc acgccccggt aaccactgat acccagcttc cagcgaggcg
     1381 ataatccccg acgaatggta acgatccacg ccatcttcat gttcggaaac atcattgtta
     1441 aaccacgcgt attgcagcca ggccccctgc ttctgattac cgtgctgata ccagcttgag
     1501 gtcagcccaa cggcataacc gtggttctgg ttatcggcgc gagttccggt catattcgag
     1561 cggctgtcgc cctgattatc actgtagcca ccaaccgccc caagcatcca ctcgccatcc
     1621 gtgccccagc gcccgctaaa caagccgccg ctaagctgca ccgtagaagt gtcttcctgc
     1681 tgagccagtt gccctgccgc cgtgtaatga taacgtccgc cgataacacg taaattcagc
     1741 gtctgaccat cgccaccagc gtgatcgcgt cgctccatca taaacgcctg atttgccgcc
     1801 cgcaggttat tgagataacc gccaactttg gcattcagca ccggctggta agcaggcgta
     1861 ggggccgggt cgggggttgg gtcaggatcc ggcgtgggat caggatcggg agtcgggtct
     1921 ggctcaggtg gcgatggcgg cgttacttct tgcgatcgca gataccagtc gttgttatct
     1981 tccactagcg tgtagtcata cgctcccatg ttgacgtagc cactgccagc cagactgaac
     2041 tgtgcattgt tttgaaactg cgtgggatca gctgcgaaat caaccacttt aatgcctgtc
     2101 gatgtcggct caccaatccc tgtaatggag ttaaccacca cagttgtgtt gccagcagta
     2161 ttaccgttca tcaccaattg atcgcttacc gagtcatcgc cgtttaattc gctgtcgaat
     2221 agtagcgtac cgccgccggt ataatcaccg tttaccgtca gcgtatcgcc agcgacgcca
     2281 ttttgcaggc taaccgcacc ggacgtattg gtcagatcac cattaaccgt cacgtcagca
     2341 agataggtca ggctgtcagt ttcaaggttg gtattaagct cgcctgtcaa cgtcacgcca
     2401 tcggtagcat ttaccagcgt cccatcgccg ttaaatagtg aggcattaag ggcgacggaa
     2461 gtatcctgcc cggtcagacg caaaaccgta ccatcgctga tgtcgatagt gccgctggaa
     2521 gtagcatcaa ttgactgaat atcctgatcc gcgccagtaa cgaacgttgc cccatcacca
     2581 acccacagtg acgaagcata aggaaagata tccgcaacat cgcctttcag ggtgccttct
     2641 tcgacacgta ccgccgactg cgttgtaccg ctggcggtca gctccagcgt acccgccccc
     2701 gttttagtaa atgtgctacc ctcatcctga tgctgcccgc tgctgtcagc catcagtgcg
     2761 ccccactgcg tgtctacccc cgcatcaact gccacttcac cgtcggcgcg catttcaata
     2821 tcacgcccat gctcagtcgt cgtagagcga gtatcgacat tggcagtaat ctccatcacg
     2881 ctttcttgtt acttatcggt aaagataacc tggcggttat agtgggtatc tccaagctgc
     2941 gagttgtcgc tcaccatcag cgtaccggag gcgatttgtg ttgtgcccaa ataactatta
     3001 ttgtttgcca gacttacctg cccgtcgctc gcatcgacca ccacgtagac gccatctttc
     3061 ccgtcgccgc cgatatgcaa attgtcgcca cctgcaagat ctaccacatc ctgactgccg
     3121 ataaccgttc cgctgctgcc actgacttca aggccatcat tgtatgatgt gccgctctgc
     3181 caagtggaga aatcagagag atcgagcaca ccgccgttta acacgatcga ctgcggatcg
     3241 tcctggagag cggtaagatc tgccgcgtcg ccttccagcg aaagcaccgc accatcatca
     3301 acgactatat cgccggttag cgccatcgac tgcgcccctg ccagcacgta gctgccgttt
     3361 tgcgcaatgg tgagctgacc agcaccttcg atgatgccag caaaactgcc ctgattaaca
     3421 gtaacgttgc caccagcatc gatatttaaa gtgccattct gaaagcccgt caatgcgtgc
     3481 acaaaagttt gttgggtcga gccaacgttt agctcagcct gattctgata ctgatcaata
     3541 ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt atcgccgaca
     3601 ttcatcaggg agttgctgcg acccagggta acttcaccgt tttcaatctg catctcacca
     3661 gtaaagtcat tgttatctgc attaagtacc agatcgccgg aacctgtttt ggtgattaac
     3721 ccggtaccag caatagagtc aacagctccg tcattctctg tattgccgat aaccagcgtt
     3781 tttccgtcgg caatatcaaa ggtaacttca cttaagccga gatacataaa gccacccgcc
     3841 gcagtggagg gaccatctcc atagcctgct gcgctattgt tctcatcgac taacacgccg
     3901 ccgttctggc tgtagctgtc atcaacagaa atatcaataa
//
LOCUS       DAJTYL010000212         3834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYL010000212 DAJTYL010000000
VERSION     DAJTYL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.214"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            465..2117
                     /locus_tag="OO572_003893"
     CDS             465..2117
                     /locus_tag="OO572_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6662698.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2128..2298)
                     /locus_tag="OO572_003894"
                     /pseudo
     CDS             complement(2128..2298)
                     /locus_tag="OO572_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2431..2550)
                     /locus_tag="OO572_003895"
                     /pseudo
     CDS             complement(2431..2550)
                     /locus_tag="OO572_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2624..3554)
                     /locus_tag="OO572_003896"
                     /pseudo
     CDS             complement(2624..3554)
                     /locus_tag="OO572_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1059 a          884 c          882 g         1009 t
ORIGIN      
        1 cacactcacg tgggtggact ggtataataa tcgacgattg ctggaaaggc tgggccacat
       61 ccctccggca gaagcagaaa aagcttatta tgcttctatc ggaaacgatg atctggcagc
      121 ctgagttcac agataaaaca ttctccagga aacttggggc ggttcacgtc agtatcagac
      181 catattaatt gctgaaactg ataaacatca aaccttatag aagggtgaat gctgaaaggg
      241 ggctatggtg atcgacgtga tgacaacgac aggtaagacc gtgacctgat aaacctgaat
      301 tgtgtcctag acataaagtc cgtgaaggga ataaggctca ggtcagcgaa tcttatagtg
      361 gcccgcagct ctgactgcaa taaggccgga tacagagcag caactactcg tcgatgtcag
      421 aacaacggaa gccttgcaaa cggggatgcg ttcatacagg aacaatgata ccgttaaaaa
      481 aaaacattac gctaataatg ttcaccctgt cattactgac aggaaatcct gctatagcgt
      541 atgaaacaga taaagtatat aaaataaccg ttctgcatac aaatgaccac catggacact
      601 tttggcgtaa caatcatggt gagtacgggt tatcctccca aaaaactctg gtggacaata
      661 tccggcagaa agtaataaat aatggcggaa gtgttctatt attatctggt ggtgatatta
      721 acaccggtgt acctgaatca gaccttcaga aggctgaacc cgatattcgt ggaatgaatc
      781 taattggtta tgatgcaatg gccgtcggta atcatgaatt tgataatcct ctaaatatat
      841 tacgtcagca ggaaaaatgg gccacatttc ctttcctttc tgctaacata tatcaaaaaa
      901 gtacagggag gcgtttattc agtccatgga aaattttcat ccgtcagaat ttgaaaattg
      961 cagtcattgg cttgacgaca gatgatactg cgaaaacagg aaattctgaa tattttactg
     1021 atattgagtt ccgtcaacct gctgctgagg cgaggagtgt cattgatgaa cttaatcagc
     1081 aggaaaagcc agatattatt attgcggcaa cacatatggg tcactatgac aatggcgagt
     1141 caggttcgaa tgctcctggt gatgttgaaa tggctcgcag tttgccgact ggctcattag
     1201 caatgattgt gggaggacat tcacaagctc ctgtctgcat ggccagcgat aataaaaaac
     1261 agtggaatta tattccgggg acaacctgtg taccagataa acaaaatggc atctggattg
     1321 tacaagcgca tgagtgggga aaatatgtcg gtcaggctga ttttgaattc tgtaacggta
     1381 caatgaaact ggttaattat cagttacacc cagttaatct caaaatgaga attaccagag
     1441 aagatggaaa aacggaattt tctttttaca cccccgaaat tactgaagat ccacagatgt
     1501 tatccttact gacaccattc cagaataaag gcaaagcaca actggatgtg aaggttgggg
     1561 ttgtgaacgg acgtctggaa ggggacagaa gtaaagttcg ttttgttcag accagtatgg
     1621 gccatctgat tctgagcgct ctgacagaac gtattgacgc agattttgct gttgtcagtg
     1681 gtggggaaat tcgagattcc atagagagtg gtaacatcac atacaaagat atactgaaag
     1741 ttcagccatt tggtaatacg gtagtaagta ttgatttgac ggggaaagaa gtagctgact
     1801 acctagctac agtggcacag atgaaaccgg attccggagc ctacccacaa tttttgaata
     1861 ccagctttgt agtcaaaaaa ggaaaaatcg aaatgctaaa aataaaaggt aaatcggttg
     1921 accttaacaa aaaatatcgt atgacaacat ttagctttaa tgctactggt ggggatggct
     1981 atccacgtat tgataataga cccggataca taaataccgg atttattgat gctgaagtac
     2041 ttatcgaata tatccggaaa cattcaccgc tggatgctgc ttcatatgaa ccaaaaggtg
     2101 aagtcagttg gcagtgataa cgtaattgac gatcatcacg tgttttttgc tgacagtcgc
     2161 tgtgactttg tgaaagtagt ctctgcgtat gtttgcgata tgggaatgca gttgctgtat
     2221 tttgtgtttc tgcttctgcc agttgttgct gaatttaacc ttgcggctta actgatgttg
     2281 gagcgctgcc agcttcttcg tcaatttcgc tgattaaagt ttattcatta tggcatgatc
     2341 tgcgtaagcg gctcgtcaga accatattga tatttactaa gagctcagat caactttcca
     2401 gggcaacaga tcgcgtaccc ggtttgccgg tcagtcctgg atatgctcaa tgacgtaacg
     2461 cagccatttt tctggctcca cattgttcag acggcatgtg ccgatcagcg agtacaacac
     2521 cgccgcatgt tcgccaccgc tatcggaacc ccgccggaga gagtttcact ctccggctgc
     2581 tcacggaacc gtacgtgaca ctctcgcgtc atacggctct tgatcactca ttcgatgcac
     2641 gtagtaaata ccagtgggca aatagcttcg gatactgttt ccttaccagc gccagctttc
     2701 gctgagcctt ccggttcgaa agtctgtatt tagactgcat ccacttaagc aaacgtgact
     2761 gcattgcact ccacagtcga tagctgaagt ttctggacca gaactttcca tagtactcaa
     2821 tccagcctct cactatcgca ttgtagcgcc tcgcaaaatc cagcaaactc tcagctgttg
     2881 agcgatgtat acgccacttc ttgattgttt ctgttatttt gcacattgct gcatttgatg
     2941 cacccggcat acattttctg tacaattcgc ctttgaaatt cttcagagta cgcactttga
     3001 agtcatatcc gagagtggca tacaacatca tcggcgtacc tttcgaaggg aacgccggga
     3061 aactgacgtt gcatccacat atcaaaggca tagtgcagga acagatttga gagcaggggg
     3121 gagattacgc cgccctgcgg cgttcctcga ccccgtgcca gcattgctcc atccggcatt
     3181 tgcaccggtg ccttcagcct gcgctcaatg tacatcacca cccagcgtgc ctgagtatga
     3241 tgctgcactg tcttcagtag caggtcatga tcgatatcat caacgaagcc tttaatatcg
     3301 acttccacca cccagtcaaa cttccagcag cgtttcctcg cctgcgcaat cgcctggtgt
     3361 gcggatttac ccggtcgata accatacgag tctgcatgga acaggggttc cagtaccggc
     3421 tcgagtatct gcttaacgac catctgggcg atccggtccg acacggccgg aataccgagc
     3481 ggacgtacac caccgtcagc tttgggaatg tccacacgcc ttaccgcctg cggcatataa
     3541 ctgccggaag ccattctgtt ccacagtttg tagagattgt ctgtaacatt ctcgtcaaac
     3601 ccggccagtg tctgcccgtc aatgcctgcc gccccacggt tggcttttac tcgtttataa
     3661 gcttcccata cctgtcgttt acttacagga aatggctttg ccgattttac agcagtcctc
     3721 ccatcggcat tgtcgcccgt ggttgccgcc gcaccatcag cacaatgggg caagcccttt
     3781 gctccggacc cattacggtc cttcaccgct actacggttt actccgtccc tgta
//
LOCUS       DAJTYL010000213         3790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYL010000213 DAJTYL010000000
VERSION     DAJTYL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.215"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1852
                     /gene="pbpC"
                     /locus_tag="OO572_003897"
     CDS             <1..1852
                     /gene="pbpC"
                     /locus_tag="OO572_003897"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6662699.1"
                     /translation="EILILYLNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAV
                     LPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLA
                     PLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSSLAMIVVNHTDMRVR
                     GWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTG
                     DYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGA
                     APNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRR
                     IMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVG
                     QFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRL
                     ATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEP
                     WLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSG
                     GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            2002..2433
                     /gene="ndk"
                     /locus_tag="OO572_003898"
     CDS             2002..2433
                     /gene="ndk"
                     /locus_tag="OO572_003898"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6662700.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2583..3737
                     /gene="trmG/rlmN"
                     /locus_tag="OO572_003899"
     CDS             2583..3737
                     /gene="trmG/rlmN"
                     /locus_tag="OO572_003899"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6662701.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
BASE COUNT          866 a          979 c         1073 g          872 t
ORIGIN      
        1 tgaaattctg atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg
       61 tgcggcaagt tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc
      121 aatgctggcg gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg
      181 tgccgaagcc gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg
      241 tgagcaggta aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca
      301 actggcaccg ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac
      361 tacgctggat gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg
      421 gttgccaccg cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg
      481 cggctgggtg ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt
      541 caatgcgatc cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga
      601 tgaaggcttg atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta
      661 tcgaccaggt aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt
      721 gcgctcgctg aacttacctg ctgtgcaggt gctggaagcc tatggaccga aacggtttgc
      781 ggcaaagtta cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct
      841 ttcactcatt ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc
      901 gtttgctcgc cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga
      961 acggccttta atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc
     1021 gcaacccttg ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac
     1081 cagctatggc tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat
     1141 ctggactggc agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt
     1201 accattgttg aatcaggtca ataatatctt actgtcgcgc agtgcgaatc tgccggaaga
     1261 cccgcgcccg aactcagtca ctcgtggcgt tatctgctgg ccgggcggac agtctttgcc
     1321 ggaaggtgac ggtaactgtc gccgccgcct ggcaacctgg ctgctggatg gcagccagcc
     1381 gccaacgctg ttactgtcag agcaggaagg gattaatggc attcgcttcc ccatctggct
     1441 ggatgacaaa gggcagcgcg tcgccgctga ctgcccgcag gcgaagcaag aaatggtaaa
     1501 tgtctggccg ctaccgctgg aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt
     1561 accaccggcc tcgacaattt gcccgccata cggtcacgat gcccagctcc ctctgcagct
     1621 gacaggcgtt cgcgatggcg cgattattaa acgtttaccg ggcgcagcgg aagcgacttt
     1681 gccgttgcaa tcaagtggag gggcaggtga acgctggtgg tttctgaatg gcgaaccgtt
     1741 aactgaacgc gggcgcaacg tgaccctgca tttgacggat aaaggtgatt atcaattgct
     1801 ggtgatggat gatgtggggc aaatcgcgac agtgaaattt gtcatgcaat agtctgtttt
     1861 gtccctgatt gttgctaaaa ctcattttat tttaaaaaaa atgttacctt catcaatagt
     1921 caacggccct gttgctcatt ataatccgcg ccatctcgta cgctggtaca gacaacaaca
     1981 gaacaattta cagaggtaaa aatggctatt gaacgtactt tttccatcat caaaccgaac
     2041 gcggtagcaa aaaacgtcat tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa
     2101 attgtcggca ccaaaatgct gcatctgacc gttgaacagg cacgtggctt ttatgctgaa
     2161 cacgatggaa aaccgttctt tgatggtctg gttgaattca tgacctctgg cccgatcgtg
     2221 gtttccgtgc tggaaggtga aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc
     2281 aatccggcaa acgcactggc tggtactctg cgcgctgatt acgctgacag cctgaccgaa
     2341 aacggtaccc acggttctga ttccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc
     2401 ggcgaaggcg aagtgtgccc gcgcacccgt taataatttc gtaaatgccg cgtgcaaacg
     2461 tggcatccgt gcgccagaat ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg
     2521 gggcgtttct tttttcaacc ctccaggggc cataacgtgt aataacgagg ccggaatagc
     2581 atatgtctga acaattagtc acacctgaaa acgtcaccac gaaagatgga aaaatcaacc
     2641 tgctggatct caaccgtcag cagatgcggg agttttttaa agatttaggt gaaaaaacct
     2701 tccgcgccga tcaggtgatg aagtggatgt atcactattg ctgcgacaac tttgatgaga
     2761 tgaccgacat caacaaagtg ttgcgcggca aactgaaaga ggtggcggaa atccgcgcac
     2821 cggaagtggt tgaagaacag cgttcatccg acggcaccat taaatgggcg atcgccgttg
     2881 gcgatcagcg cgtcgaaacg gtgtatatcc cggaagacga ccgtgccacg ctctgcgtct
     2941 cttcgcaggt ggggtgtgcg ctggagtgta aattctgttc caccgcccag cagggcttta
     3001 accgcaacct gcgggtatcg gaaattattg gccaggtgtg gcgtgcggcg aaaatcgtcg
     3061 gcgcagcaaa agtcaccggt cagcgcccaa tcactaacgt cgtgatgatg ggcatgggcg
     3121 agccgctgct caacctgaac aacgtcgttc cggcgatgga aatcatgctt gatgatttcg
     3181 gttttggcct gtctaaacgt cgcgtgacgc tttccacttc cggtgtagtt ccggcgctgg
     3241 ataaactggg cgatatgatc gacgttgcac tggcaatttc cctgcacgcg ccgaacgacg
     3301 aaattcgtga cgaaattgtg ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg
     3361 cggtgcgccg ttatctggag aaatccaacg ccaatcaggg ccgagtcact attgaatacg
     3421 tgatgcttga ccacgttaac gacggcactg aacacgcgca ccaactggcg gaactgctga
     3481 aagatacgcc gtgtaagatc aacctgatcc cgtggaaccc gttcccggac gcgccgtatg
     3541 gacgcagttc gaacagccgt atcgaccgtt tctcaaaagt gctgatgagc tacggtttca
     3601 ccactattgt gcgtaaaacg cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg
     3661 gcgatgttat cgaccgtacg aaacgtactc tgcgtaaacg gatgcagggt gaagccatcg
     3721 atattaaagc ggtctgatag ccattacgcc acggttactt tatgtaccgt ggcgtaatct
     3781 tttatgaatg
//
LOCUS       DAJTYL010000214         3717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYL010000214 DAJTYL010000000
VERSION     DAJTYL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.216"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..213
                     /locus_tag="OO572_003900"
     CDS             <1..213
                     /locus_tag="OO572_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6662702.1"
                     /translation="DRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQ
                     NEARQQKRGLWSDADPVPPWIWRHRK"
     gene            259..468
                     /gene="hha"
                     /locus_tag="OO572_003901"
     CDS             259..468
                     /gene="hha"
                     /locus_tag="OO572_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6662703.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            507..1097
                     /locus_tag="OO572_003902"
     CDS             507..1097
                     /locus_tag="OO572_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6662704.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1337..1597
                     /gene="repA"
                     /locus_tag="OO572_003903"
     CDS             1337..1597
                     /gene="repA"
                     /locus_tag="OO572_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6662705.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1821..1895
                     /gene="tap"
                     /locus_tag="OO572_003904"
     CDS             1821..1895
                     /gene="tap"
                     /locus_tag="OO572_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6662706.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1888..2745
                     /gene="repA"
                     /locus_tag="OO572_003905"
     CDS             1888..2745
                     /gene="repA"
                     /locus_tag="OO572_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6662707.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3446..3637
                     /locus_tag="OO572_003906"
     CDS             3446..3637
                     /locus_tag="OO572_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662708.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRVIQAAFNAFAKV"
BASE COUNT         1016 a          905 c          933 g          863 t
ORIGIN      
        1 gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa tattaatcag
       61 tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga tcccgtgcta
      121 cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga tgctgatccg
      181 gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat cattcatctg
      241 ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca gttacggcga
      301 tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag tttatcaaac
      361 agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt aatcaccgga
      421 aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata attcagcaat
      481 aattaattgc caggagcaga taaataatga agttgattat ttttattctc attgttctga
      541 ttattgcagc gctacttatc cggattatcc tcagaagtgt aaatcagcat tctcctttgc
      601 tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata ctgtccagcg
      661 gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt tttatgaaag
      721 gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta cgggtagcgg
      781 atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg cagttattca
      841 ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg ttcagtattg
      901 ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga cgccgggata
      961 ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc cacgatgcca
     1021 gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat cagcagtcac
     1081 cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga aacgggaaaa
     1141 agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt tcacagttta
     1201 aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat cttcgccaca
     1261 attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga
     1321 gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa
     1381 gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg ccgctgttgc
     1441 aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc tgaaagatct
     1501 catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg agaaaatcat
     1561 caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg tggcgtagca
     1621 tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag gtggcaggga
     1681 actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata atcttcttta
     1741 actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca acaatcaagc
     1801 tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg tgctcgctcc
     1861 ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta ttaccgccag
     1921 gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct tccgttctgc
     1981 gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg
     2041 gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg tcgccgggcc
     2101 attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa ccgcgtccag
     2161 tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc tgccggaaaa
     2221 ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg actgattacc
     2281 taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca
     2341 tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc gcgccgcagt
     2401 cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct gggtatggat
     2461 gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag
     2521 cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag gcagcgtcag
     2581 gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg gcgcttcact
     2641 gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg
     2701 tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg acctcctcag
     2761 aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg cacaaaaaat
     2821 cagcgctaca tacaaaaaac aacctcatca tccaccttca ggtgcatccg gtcccccctg
     2881 tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat taaactgcaa
     2941 gggacttcac cataaggtta caaccgttca cgtcataaag cgccagacgt caaccctaca
     3001 gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt aattacattc
     3061 atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacaaatatg cacctcccac
     3121 cgcaagcggc gggcccccaa ccgaagccgg tttagttaca acatgcaaac ataaacccct
     3181 aaaaaaccca agtccaacgc aaaactgaaa ccacaacgcc cccctcataa ctgaaaagcg
     3241 ccgccgcccc cgcccaaagg gcgggaacag agtcgctttt aattatgaat gttgtaacta
     3301 catcatcatc gctgtcagtc ttctggctgg acgtatcgaa tacacgctcg taagcggccc
     3361 tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg tcgggaccac
     3421 tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg cagggctggg
     3481 gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg gcggttttac
     3541 tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg gcatcagagt
     3601 aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg gtcgtttcag
     3661 ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa aaaaaca
//
LOCUS       DAJTYL010000215         3598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYL010000215 DAJTYL010000000
VERSION     DAJTYL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.217"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            20..748
                     /gene="tonB"
                     /locus_tag="OO572_003907"
     CDS             20..748
                     /gene="tonB"
                     /locus_tag="OO572_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6662709.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(788..1186)
                     /gene="yciA"
                     /locus_tag="OO572_003908"
     CDS             complement(788..1186)
                     /gene="yciA"
                     /locus_tag="OO572_003908"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6662710.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1291..1830)
                     /locus_tag="OO572_003909"
     CDS             complement(1291..1830)
                     /locus_tag="OO572_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6662711.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1860..2603)
                     /locus_tag="OO572_003910"
     CDS             complement(1860..2603)
                     /locus_tag="OO572_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6662712.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(2631..2720)
                     /locus_tag="OO572_003911"
                     /pseudo
     CDS             complement(2631..2720)
                     /locus_tag="OO572_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2960..3598
                     /gene="ompW"
                     /locus_tag="OO572_003912"
     CDS             2960..3598
                     /gene="ompW"
                     /locus_tag="OO572_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6662713.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIAIVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         1008 a          874 c          881 g          835 t
ORIGIN      
        1 tttttctact gaaatgatta tgacttcaat aacccttgat ttacctcgcc gcttcccctg
       61 gccgacgtta ctttcggtct gcattcatgg tgctgttgtg gcggggctgc tctatacctc
      121 ggtacatcag gttattgaac tacctgcgcc tgcgcaacca atttctgtca cgatggttgc
      181 gcctgctgat ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc
      241 agaaccggaa cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa
      301 gccgaagccg aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa
      361 acgcgatgtc aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcacccgc
      421 gcgccccaca tcaagtacgg caacggctgc aaccagcaag ccggttacca gtgtggcttc
      481 aggaccacgc gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg
      541 cattgaaggg caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt
      601 acaaatcctc tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg
      661 cagatggcgt tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa
      721 aattaacggc accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt
      781 ttgactatta ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gctacatact
      841 taaataatgc ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca
      901 cccacacttc aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc
      961 agcacaccac atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacag
     1021 tcactacgcg accgtgggca atttcttttg ccagaatagc gccgccaata tccatttgtg
     1081 acattaacca accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag
     1141 tacgtaaaac aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt
     1201 aaaaggaaaa tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac
     1261 agcgccagcc ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg
     1321 gtagatatag ataccgctta acaatgtgaa gattaaggta agggcggtca ggccaaagac
     1381 tttaaagttg acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc
     1441 gcaaaggata aagaaaacag cccaggccag atttagcttc gaccataccg gttgcggcag
     1501 cgtgagttct ttactcagca tccgctgaat tagcggcttt ttcatcaccc attggctgac
     1561 taacagggct cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc
     1621 attgtggaag aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc
     1681 catcttctca accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac
     1741 gatcagcgct gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac
     1801 cagcggtaaa aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt
     1861 tattggcgaa tcaacatata caggcggaac agatagatga gcaatatggc agaaatcagg
     1921 ttgctcaagg tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag
     1981 gcaaaaagca gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga
     2041 cgcatattcg cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg
     2101 tcctgtacca gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg
     2161 accaccagca tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac
     2221 ttgggcaata tcggcgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca
     2281 gacaccagct ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaattct
     2341 gaaaaagtgg acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc
     2401 tgaaccaggt cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca
     2461 agctgtgcat cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc
     2521 aacgatacca acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat
     2581 acggactgcg ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg
     2641 ggcaattgta ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata
     2701 tctttgtaaa gcggaggtaa ttgctggcct tcaggcgtgg gcaaagcgcg gcaagtggcg
     2761 atcgagttaa tatttgcgta gagaaaatat ttaaaaattg atttaaatca cattaaccag
     2821 gattctcaat gcaacttcta aattaatcca gatcaataaa tggtgaatta tcatatgtaa
     2881 tgtgatctat gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa
     2941 ccataacgac ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct
     3001 tctttctggc agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc
     3061 aaccgtacgt ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt
     3121 gaccaataac actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt
     3181 ggaattactg gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat
     3241 tgcaatcgtt caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag
     3301 cagcaaattc cgtccttacg ttggggccgg tattaactac accaccttct ttgataatgg
     3361 atttaacgat catggcaaag aggcggggct ttccgatctc agtctgaaag attcctgggg
     3421 agctgccggg caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc
     3481 agtgtggtac atggatatcg ataccaccgc caattataag ctaggcggtg cacagcaaca
     3541 cgatagcgta cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaa
//
LOCUS       DAJTYL010000216         3478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYL010000216 DAJTYL010000000
VERSION     DAJTYL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.218"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(39..686)
                     /gene="ychE"
                     /locus_tag="OO572_003913"
     CDS             complement(39..686)
                     /gene="ychE"
                     /locus_tag="OO572_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6662714.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1163..>3478
                     /gene="adhE"
                     /locus_tag="OO572_003914"
                     /gene_synonym="adhC"
     CDS             1163..>3478
                     /gene="adhE"
                     /locus_tag="OO572_003914"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6662715.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQAR"
BASE COUNT          949 a          875 c          801 g          853 t
ORIGIN      
        1 taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag gccggggaaa
       61 atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa tagccccata
      121 atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca cggtgccatg
      181 cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa accaaacaga
      241 tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc ccctggcccc
      301 gccatcaacg gcaacgccaa tggcaccaca ccaatacttt cacgtaccgc ggtttctgat
      361 ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga catcgctatc
      421 gtcaccacca ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc aaaaagttgt
      481 agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga caggttggct
      541 gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct gataaagacg
      601 ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt gaagtaaacg
      661 ggaaaatcaa aaagggtctg aatcacggtt cgctccgaag caaaagccgg ataatgttag
      721 ccataaataa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat ttttcacctc
      781 ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa acatgctaat
      841 gtagccacca aatcatacta caatttatta actgttagct ataatggcga aaagcgatgc
      901 tgaaaggtgt cagctttgca aaaatttgat ttggatcacg taatcagtac ccagaagtga
      961 gtaatcttgc ttacgccacc tggaagtgac gcattagaga taataactct aatgtttaaa
     1021 ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt catcggttaa
     1081 gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta ctaaaaaagt
     1141 ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta acgcactcgt
     1201 agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc aagtagacaa
     1261 aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg cgaaaatggc
     1321 cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc actttgcttc
     1381 tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt ctgaagacga
     1441 cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta tcgttccgac
     1501 cactaacccg acttcaactg ctatcttcaa atcgctgatc agcctgaaga cccgtaacgc
     1561 cattatcttc tccccgcacc cgcgtgcaaa agatgcaacc aacaaagcgg ctgatatcgt
     1621 tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga tcgatcaacc
     1681 ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga tcctcgcgac
     1741 tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta tcggtgtagg
     1801 cgcgggtaac actccagttg ttattgatga aactgctgat atcaaacgtg cagttgcatc
     1861 tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt
     1921 tgttgttgac tctgtttatg acgcagtacg tgaacgtttc gcaacccacg gcggctatct
     1981 gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaatg gtgcgctgaa
     2041 cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct tctctgtacc
     2101 agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc
     2161 acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg aagacgcggt
     2221 agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt gcctgtacac
     2281 tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga aaacagctcg
     2341 tatcctgatt aacaccccgg cttctcaggg tggtatcggt gacctgtata acttcaaact
     2401 cgcaccttcc ctgactctgg gttgtggttc ctggggtggt aactccatct ctgaaaacgt
     2461 tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa acatgttgtg
     2521 gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg cgctggatga
     2581 agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa
     2641 tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt
     2701 cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag aactggcaaa
     2761 ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa
     2821 gatcatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg cgctgcgctt
     2881 tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat
     2941 cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga
     3001 cgacgctact ggtcagaaat acccgctggc ggactatgcg ctgaccccgg atatggcgat
     3061 tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg gtggtctgga
     3121 cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt tctctgatgg
     3181 tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct accacgaagg
     3241 gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg cgggtatcgc
     3301 atttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt
     3361 ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc gctacaatgc
     3421 gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc aggctcgc
//
LOCUS       DAJTYL010000217         3402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYL010000217 DAJTYL010000000
VERSION     DAJTYL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.219"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO572_003915"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO572_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6662716.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO572_003916"
     CDS             complement(687..1160)
                     /locus_tag="OO572_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6662717.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO572_003917"
     CDS             1492..2028
                     /locus_tag="OO572_003917"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6662718.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO572_003918"
     CDS             2143..2469
                     /locus_tag="OO572_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662719.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO572_003919"
     CDS             complement(2657..2896)
                     /locus_tag="OO572_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6662720.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          941 a          752 c          717 g          992 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc cc
//
LOCUS       DAJTYL010000218         3363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYL010000218 DAJTYL010000000
VERSION     DAJTYL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.220"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(255..899)
                     /gene="cheZ"
                     /locus_tag="OO572_003920"
     CDS             complement(255..899)
                     /gene="cheZ"
                     /locus_tag="OO572_003920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6662721.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(910..1299)
                     /gene="cheY"
                     /locus_tag="OO572_003921"
     CDS             complement(910..1299)
                     /gene="cheY"
                     /locus_tag="OO572_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6662722.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1314..2363)
                     /gene="cheB"
                     /locus_tag="OO572_003922"
     CDS             complement(1314..2363)
                     /gene="cheB"
                     /locus_tag="OO572_003922"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6662723.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2366..3226)
                     /gene="cheR"
                     /locus_tag="OO572_003923"
     CDS             complement(2366..3226)
                     /gene="cheR"
                     /locus_tag="OO572_003923"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6662724.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3245..3358)
                     /locus_tag="OO572_003924"
     CDS             complement(3245..3358)
                     /locus_tag="OO572_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6662725.1"
                     /translation="MANQADHLSSRVAVFTLEEHEVARHESAQLQIAPVVS"
BASE COUNT          796 a          983 c          774 g          810 t
ORIGIN      
        1 atttcttgca ccatgaactg acgaaaaaac gttatgaagt atggcatcac tacgatgtac
       61 tgcactacca gtcatcgtca ttttctcgtt cacaatttaa tgcattgata aatttactgg
      121 cgcaacaatc acgcttcgct gatctaaaat tatcaaacta caaataacca tcctccacgt
      181 actccctgaa cgatacccgc caccctgcta tcggcttggc atcatcgcgc aggccgaagt
      241 tcaggcaata caaatcaaaa tccaagacta tccaacaaat cgtccacctg atcctgactg
      301 gctaccacgc cggctttgct ggtatcgacc tgaggtccat taagcaaact ctggttttca
      361 cgttttggac gcgactcctg ttccgggatg ttttccaaca gcaccatcag caactggcgt
      421 tcgatctcct gaatgacatc catcatccgc ttaataacct gcccggtgag atcctgaaaa
      481 tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa agctggtatg cgcgggtaca
      541 tctgccagaa attgtcgtgt atctgttacc agttcacggg cgtcggcaag gtcaatcgga
      601 tcggcaaacc agtcatccca acgttgggtt aacgcttttg ctgatttctc catttgatcc
      661 tgatgcggtt gtgacgcctc aacactgttc agcgcccgct ccgcagcctg ggcggtcatc
      721 tgcacaacat agtacaaacg atcgcgcgca tcggggatgg cttccgccgc ttcggcaatg
      781 gcctgaacca gccccagttc ccgcaaactg tcgcgcagca tatgcgtcag gctgccgatg
      841 cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata
      901 gtcgcatcct cacatgccca gtttctcaaa gattttgttg agtttttcct ccagcgtcgc
      961 ggcggtaaat ggcttcacca catagccact ggcccccgct tgcgccgcag caatgatgtt
     1021 ctctttcttc gcttctgcag tcaccattaa cactggcaat gccgacatcg cgccatccgc
     1081 acgaattgtt ttcagcaatt ccaggccatc catattgggc atgttccagt cggagataac
     1141 aaatccataa ccgcctgcct gcaacttatt gagagcgtcg acaccatctt ccgcttcctc
     1201 aacattattg aatcccagct ctttcagcag gttacgcact atgcgtcgca tggtggaaaa
     1261 gtcatccaca accaaaaatt taagttcttt atccgccatt tcacactcct gatttaaata
     1321 cgtatcgcct gtccggcact aattgttgcc aacatttgct ggcttacctg gctaagatcg
     1381 accacttcgc agacaccacc catattgatg gcctcgcgcg gcatgccgaa caccacgcaa
     1441 cttgcttcgt tttgcgcaag ggtccatgcc cccgcctgac gcatcgccaa cattcccgcc
     1501 gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg cattacgccc cgcctgtttg
     1561 gcgacagaat ggaacaacac atctaccgaa ggccgatgac ggttaaccgc cgggccgtcg
     1621 tgaattttga tttgataatt tgcgccacta cgcgccagtt ccatatgccg atcgcccggc
     1681 gcaatatagg catgccccgg caagacacgt tctccgtctt cggcttcttt aaccccgatc
     1741 tggcaaagct tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg catatgttgg
     1801 gtaattaaca atgccgggct ggaaagcggc aacggttgca gtacgtgacg aattgcctca
     1861 gttccacccg ttgaagcacc aatcgcaatc agtttttcag aactcaacaa cggccctgcc
     1921 ttcagcgttg tcggtgctga caatggctta tgtgctgcaa ggctcgcctt tgctgccgta
     1981 cgcacctttt cagcaatcat ttcgctatac gccagcattc cttcgcgaat acccagttgc
     2041 ggtttggtga caaaatctat tgcccccagc tccagcgcgc gcagcgtgac ttctgatcct
     2101 ttgccggtca aggaagaaac catcacaacg ggcattggac gcaaacgcat taatttttcg
     2161 aggaaatcca gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac atcgggattg
     2221 aatttcttaa tcaagtcacg cgcaaccagc ggatcaggcg cggtcgccac catttccatg
     2281 tcgctatggc tgttgatgat ttctgtcatg atctggcgca tcagtgccga atcatcgaca
     2341 gataacaccc tgattttgct catcgttaat ccttacttag cgcatacacc gtctgaccac
     2401 gcagcgtgaa gcggcgctcg aggtggctaa agttttcaga gtgacccgca aacagcaatc
     2461 cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc ctgctgggta gtttgatcga
     2521 agtagatcat gacgttacga cagaagatcg catcaaacgg ccccggcacg gtgtactgtt
     2581 tcgccagtaa attcagcggg gcaaaatcaa catagttcgc cagctcctga cgcacgcgta
     2641 ccagcccttc atgcggtccc gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca
     2701 ggtttttcaa ctcttcatgg cgatagatac cgcttctggc tttttccagc acttcggtgt
     2761 cgatatcact ggcaaacact ttccagcgtc cgggcacggt gcccaatgtg tcagccagag
     2821 tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc cgcgctccat acgcgatcct
     2881 cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa atgatgagcc tcacggaaaa
     2941 atgccgtcag attcgtggtc agcgaattga taaacgcctg ccactcaccg ctgtgctgat
     3001 tagattccag caagttcaga taatgaccga aatccgtcag tcccagagaa cgcaaacgac
     3061 gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc cagaacgatc ccggctcgtt
     3121 gatagatcaa ttgacttatc cgccgaaaat gcgcgtcgga aagcgccagg cgctcggtca
     3181 tctgtaacaa taaagacgtt tgcccacagg gcagagatga agtcatagcg ccttctcaat
     3241 cacttcagga taccactggc gcaatttgta actgcgccga ctcatgtcgt gctacttcat
     3301 gttcttcaag ggtaaatacc gccacacgcg acgaaagatg gtcggcctga ttcgccagtt
     3361 gtt
//
LOCUS       DAJTYL010000219         3353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYL010000219 DAJTYL010000000
VERSION     DAJTYL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.221"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1071)
                     /locus_tag="OO572_003925"
     CDS             complement(<1..1071)
                     /locus_tag="OO572_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6662726.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETF"
     gene            complement(1156..1491)
                     /locus_tag="OO572_003926"
     CDS             complement(1156..1491)
                     /locus_tag="OO572_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6662727.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1491..1847)
                     /locus_tag="OO572_003927"
     CDS             complement(1491..1847)
                     /locus_tag="OO572_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6662728.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(1847..3343)
                     /locus_tag="OO572_003928"
     CDS             complement(1847..3343)
                     /locus_tag="OO572_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6662729.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT          749 a         1024 c          775 g          805 t
ORIGIN      
        1 gaaggtttcc cccagcgtgg aagaaatcgc attgatctgg ttctgcagca gcaacaactg
       61 attttctgtc gtcgcggcac gggcggcata ttctttctgc attgagccgc catactgctg
      121 cgcatccgcc acacgattaa agttggttcg caacaaatcc agattcgtga gcagcggcgc
      181 aatggccccc agggattcct tcccgaacag cgctttcagc acagcagcct gtttttcttt
      241 cggcactttt gccagagaat ccagtacgtg cagcatggcc ccgcgggcgt ctttctgcat
      301 atccgccgcc agttttttcg ggctaatgcg caacgagcgg agcacttttt tctgcgattt
      361 ggtggcagaa tccctcgctg tcagcgacaa cataaaattc tttatccccg tcgcagcaat
      421 ttctgactcc acccccattc cggcaatggt tgcccccatt gcggcaatct ctccggaggc
      481 cacacccgca acactgccta aagggccaat acgggtcaca acatcagaaa tctttttcgc
      541 acttgcagga ccggtattgc caagatagtt aatcttatcc gcaagccctg ccacctctcc
      601 ctgtgtcagt ttaaaggcag tgcgccactg cgccatcatc tgaccggatt cttctgccgt
      661 ggtgtcaaag gccacgccca tcttcacggc atcgtcagta aactgcatca gctcgtcacg
      721 ggcgatacca gcctgaccac ccgccgccac aatttcggcg ataccttccg ccgacatggg
      781 cagttctgtt gacaggtcgc gcacctgctc cgtcattgcc ttaaacgctt ccggcgtatc
      841 cagaccatcc accactttcc ggacatccgc catttttgat tcaagggcaa tggcagattt
      901 gaccgggagc gccagcgccc ccagtactgc ggttccggca cccgctgcgc ccagtgacag
      961 gctggcaaac tcctttttaa aacctttcag ctggcgctgc attcccttca gcggtgccga
     1021 taccttatcc acggcagtga tgatcgcctt cagctgaaaa ctgtcagcca tgcttcatct
     1081 cctcgttaat gcggacggcc tcggcttcca gttctgtaaa ctgcgaaaca gccacccgtc
     1141 gcagctccag tggattcagt ttccagaacc aggcaacatt gtagagtcgt ttccggaggt
     1201 gcttcccgtc tccgactggg taaaaaaacg caggatctgc atgctggtct taaaaatatc
     1261 cagttttgcc atctgcgccg ccgatgaaca cgggatcccc gccagcaacg ggatatattt
     1321 cagtgccacc tggctgtcca gtttaatact gccttcaccg gaaataatga aaggaaaacc
     1381 cagcgcctcg atttcgtcat acgtgggctc gcgcaactcc agcacatgca gcatttcgtt
     1441 atgcgctgtc accggttttt ttaaaactat ttctgtaacg ttcattgcca ttactgatac
     1501 cctccctctt cgccgtggaa ttcaagatct gccgtgcctt cttcggcatt atgattagcc
     1561 tctccgtgca gccatgccgc cgaaagcaca tacaccatgc cgtttgccag ctcagcggta
     1621 atcgtcatct ggtctgaggt ggtaattttg tcgaccggaa agtttttggg cactttaaac
     1681 gtgcccttaa cgtaaggtga ccgccaggtc tccttgtaat ccacatcccc tgccatgccg
     1741 acgatgtcat cccggacatt ggtgttcatc ggcacttcaa tgccacctgt cagcgataac
     1801 tgctgaccat ccactttaaa aaaacaggtt ccggcgatct ttgccattat gctgactcct
     1861 ctgaatactg aagacggaac tggttaacca ccgcaaagac acgtaactga ttaacataat
     1921 ccggcgggaa cagcgtattc agtcggttcg ggttatctgc atcacgctca actatcagat
     1981 actgtttaaa cagatcgtat ttttccacaa taccggcacg ctccatctga cgatatgtcg
     2041 ccagaagttc ccctttgata acggcaggag tgacaatcgc ctgccccgga ccaaaacggg
     2101 taccatcatt tgccagcttg tgacgtccgt acttgcttgt gatgaccgat ttcagtttgc
     2161 gaagaacata tgcactggta tgcagagttt cactgtccag atagctgttg tctgccacgc
     2221 catacgcatt ctttttgtag gtggttacgg aacgctggat ctgcaacgtg ccgccttcca
     2281 cataagccgt cgccacgccg tgagataaaa gggtctgctg ctctgtcatg atgaatcgct
     2341 tacctttcgg tgccggaagc atccccacca gctcccccgt ctgtgtcgga cgggcaggat
     2401 cattccggat aaataccgct tcacgggcaa ggcgactggc aaccagttca tcgacaggcg
     2461 actgggtttc tttttcgtaa ccggcaagcg tgatatgttg ctgattatgc atatctccgg
     2521 caccaaccag ctctgacagc gttcccagtt ttgcggtata gacatgcccg tataactggc
     2581 gcgcataact ccagcgaccg ctgctgtcat tcatttcggt catcatcata ttgatggagg
     2641 cggcatcgtt gaacggcaga ccgataaagt cgaatgcctc atcgcccata gcagcaacag
     2701 cggcggtaag atcaggcgct ccgctaccgg cagcaccgac ttccgtcacc acccgaagcc
     2761 ctgctggcaa aatctcacca ccaccagaac cataataatt cagacagacc ggtaactcat
     2821 taccatacag ccccttatga cgggcagtca gcgtcaccac gcctgcatca gatgacgccg
     2881 taaacggcag ggtgataacc ccgtttaccg cttccttaat cgcggtggca accgcagtgg
     2941 catcatcgcc attcaccaca ggcacctgta cacgggagcg cccgacatac agactcaggg
     3001 tgccgctttc ctctgcttct ccggcaaccg tcaccctgac cgtcgccgcc gcccctctgg
     3061 cttccggtac tgcaataaca tacagttcac cgaaaggatc tgtctgacgg tagacttcca
     3121 ccatacgcgc cagctggctc cccgccccac aaatctggcg ggcataatct gccgatggca
     3181 tcagcaccag gctgttaacc tcaatggctg catcgttgct ggcatgcccg atcagtaatg
     3241 caggcgcgct ggtcaccgct gtatttgccg cagaattgtt catctcggcg tagaacaacg
     3301 gcaccagcgt attcgacgga acagtattaa aacttatcgt catgattttt tag
//
LOCUS       DAJTYL010000220         3329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYL010000220 DAJTYL010000000
VERSION     DAJTYL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.222"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            10..888
                     /locus_tag="OO572_003929"
     CDS             10..888
                     /locus_tag="OO572_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6662730.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            885..2276
                     /locus_tag="OO572_003930"
     CDS             885..2276
                     /locus_tag="OO572_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6662731.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2273..2530
                     /locus_tag="OO572_003931"
     CDS             2273..2530
                     /locus_tag="OO572_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662732.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2625..2825
                     /locus_tag="OO572_003932"
     CDS             2625..2825
                     /locus_tag="OO572_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6662733.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2818..3198
                     /locus_tag="OO572_003933"
     CDS             2818..3198
                     /locus_tag="OO572_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6662734.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          879 a          722 c          965 g          763 t
ORIGIN      
        1 gtgacagcga tgatgacgtt taacctgcgt gaacaacaaa aaagactaca ggcgcgaatg
       61 gatgagttac gggcagagat tgcatttgct cagaagggcg aaaagccatg gccttatcgt
      121 tcctgcctga tgcgtgaagg tcgcggatat tgcgaaaaac atggcgaata tcacacgcat
      181 atactggtgt ggagcgatcg taatggcgag gacagagaag aaatttcatg ctgccctgac
      241 tgcttaatcg ctgaggccaa cgatttgacc atggagctgt cgtccatcaa ggcggaagag
      301 ctgactgata acgccggaat tgccctgcgt tttcgggact gcgagtttga taattatctg
      361 gaggttaatc ctggcgcagc cagaaatctt gcggcctgtc gccgctatgc ggagaactgg
      421 ccagatatgc tggagaacgg taccagtctt gttatgaccg gcagttgtgg taccggaaaa
      481 aatcatctgg ctgtggctat ggcaaaacac atcatccgta actatctggc cagtgtggag
      541 atcaccgacg tgatgcgcct tacccgtgct gtgaaaaatt gctggcggaa tgacagcgaa
      601 aaaacagcgg atgaagttat tgagcgttat gcgtcaatgg atttgctgat catcgacgaa
      661 gttggcgttc agtttggcag cgcggctgaa atggctattt tgcaggaaat tatcaacgcc
      721 aggtacgaaa gtatcttgcc caccattctg atcagtaacc tctcaccgga agagttgtgg
      781 gcgttcatca gtccccgaat tgccgacagg atcacagacg ggggacgcaa ctggttgtcg
      841 tttaactggc ccagttaccg ttctcgtatc agaggtgtgg ctgcatgaca actccagtat
      901 ggcgtaacga tgaccttgaa ggcgctgtca tcggcgcgtt ctttctgcgc ggagcagatc
      961 ctgaagtgat ggatattctg gccacactgc cagcggacgt tttttctgta cgagcgtatc
     1021 aggatatcta tacaggcatc tgcagacagg cccgtgtttc aggagtgatt gaccccgtgc
     1081 tgttgtgtaa tgagatgccg gaacttgccc cggtgattac tgataccggg cgtaaaacct
     1141 gggtgaagtc ttcactggag cactatgttg cagcgttgcg gcgcaatgcc gcactgcgcg
     1201 atgcagaaaa aacactgaat gaggcgctgc agaaattacg tgatgcgcat acctgtgaag
     1261 cagctgaaga tgccctgaag gatgcgcaga acatgatggt cacactgtcg acaggaaagg
     1321 gcgtcattca gccggtacat attgatgatg tgcttccgga agtggttgag cgtgttgaat
     1381 gccggaatca ggggctggag aaatccagga cgttgatgac cggtattgat gaactggacg
     1441 caaaaacagg cggtatggag cccggagacc tggtattcat tgccgcccgt ccttcgatgg
     1501 ggaaaaccga acttgcgctg gacatcatcg acaaggtgac tgagcagggg catggtgtgc
     1561 ttctgttcac catggagatg gcgaacatcc agattggtga acgtatggtg tctgcggctg
     1621 gagggatgcc agtatcacgc ctgaaatctg tggctcactt tgaagatgaa gactggacgc
     1681 gtttctcaca aggggtgggg cggatgaccg ggcgcaatat ctggatggtg gaccaggcga
     1741 acctggccat tgatgagata tgcgcaacaa cgaaacacca cctgattaaa tatccggaaa
     1801 cggcgctggt ggtggttgat tatctcgggc tgataaaaac ccgaaccacg gggcgtcatg
     1861 accttgccgt gggtgaaatc tcaaaggggc ttaaaggcct ggcaaaatcc ggtggttttc
     1921 cgttgattgc gctgagccag ctctcccgcg gtgtggagtc cagacccaat aaacgtccca
     1981 tgaactcaga cctgaaaaat tccggagaaa tagaggcgga tgcagacatc attctgatgc
     2041 tttacaggga tgaagtgtac aacccggata cgcaggccag gggcatcgca gaaatcaata
     2101 tcacgaaaca acgtaacggt tctctgggaa cgatttaccg gcgtttttat aacggacatt
     2161 ttctgcctgt ggaccaggaa agtgcacggg tgctttccac tcccatgaag cccggcaatc
     2221 cgcgcagata cagtaataaa cgaactgaca gcagtaagat ggagcgtttc ttttgaacaa
     2281 ccagacaatg acttttaccc ctgaacaatt gcgtaaacac gcgcaggaaa tgctgcgtca
     2341 tgctgaacaa ctcgaaaaaa cgggcataac aaaagatgct atccgtaaag atatggtacc
     2401 cgcgcttcgg gaactgatgc aggcgaagca tcgcgcacag aaagcagtag atgagctggt
     2461 ggactgtgtg gcagagctgg aaaccagagt cggaaagttt gaaaaactgg tgcaggaggc
     2521 gctgcgctga tgcgccatga gtttgttttg ccctggcttg tgcgcgatca ttatgtccct
     2581 ggtggaaagc ccgggataaa aatcaccgaa ccggagagta tttgatgagc gtcaaaattc
     2641 aaacgatacc ggaactgctt atccagacac gaggtaatat gaccgaagtt tcacgaatgc
     2701 tgaactgtaa tcgcgccacg gtaagaaaat atgctgaaga taaagagggc aaagggcacg
     2761 ccattgttga tggtgttctg attgttcatc gtggatggga caggggtaaa gacagtgatg
     2821 cgtgatattc agcaggttct tgagcgctgg ggcgcgtggg tggctaataa tcatgaggat
     2881 gtgacctggt cgtccattgc tgccggtttt aagggattaa tcccttcaaa agtaaaatcc
     2941 cgcccgcaat gctgtgacga tgacgcgatg gttatatgtg ggtgtatggc ccgccttaac
     3001 cggaacaaca acgatctaca tgacttgctg gttgactact acgtgtttgg gatgacattc
     3061 atgacgctgg cgcggaagca tggatgctca gatggttata taggaaagaa gctacaaaaa
     3121 gccgaggggg tggtggaagg aatgttgatg atgttgggtg tccggttaga aatggacaaa
     3181 tatattgaac atccctgatg gtaaggggcg actttctaga aaatatttta cgtacgtaaa
     3241 aaacgggata ttctgttaag agtggttgct tcgccacaca gcttaaaccc gcctccgggc
     3301 gggtttttta tgcctgaaaa acggtacag
//
LOCUS       DAJTYL010000221         3292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYL010000221 DAJTYL010000000
VERSION     DAJTYL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.223"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1757)
                     /locus_tag="OO572_003934"
     CDS             complement(<1..1757)
                     /locus_tag="OO572_003934"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6662735.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVAL"
     gene            1889..2998
                     /gene="apbC"
                     /locus_tag="OO572_003935"
     CDS             1889..2998
                     /gene="apbC"
                     /locus_tag="OO572_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6662736.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          786 a          822 c          868 g          816 t
ORIGIN      
        1 atcagcgcca cgcgcaggtc aactttggcg aagtcgtcaa aggtgatggt ttcctgaatc
       61 ggatcatctg ccagtgggcc agtaactggc gcggtagtgg ctttcacttc ttctttagag
      121 gcttccacca gagcttcaac ctgtttcata tcgatgcggt tatacagcgc cttgaacgga
      181 ttcactttgt ggcccagcag cggttgctgg ataccatccc aggtcagttc cgtattgagg
      241 aaggcttctg cacgctcggt cagtttcggc agtacaggct tcaggtaagt catcagtacg
      301 cggaacaggt tgatgcccat tgagcaaatc gcctgcaggt cagcatcgcg accttcctgt
      361 tttgcgacta cccacggagc ctgctcgtcg acatagcggt tagccaggtc agccagtgcc
      421 atgatttcgc gcactgcttt accaaattca cggctttccc acgcttcacc aatcacttca
      481 gcggcatcgg taaaggtttt gtacagctca gggtcagcca gctcgcttgc cagcacaccg
      541 tcaaaacgct tgttgataaa gcccgcatta cgggaggcca ggttaaccac tttgttcacg
      601 atatcggcat tcacacgctg aacgaaatct tccaggttga gatcgatatc atcaatgcgc
      661 gaagagagtt tcgcagtgta gtagtaacgc aggctgtcag cgtcaaaatg attcagccag
      721 gtgctggctt taataaaggt gccgcgagac ttggacatct tcgcgccgtt caccgtcaca
      781 tagccatgaa caaacaggtt ggtcggcttg cggaagttgc tgccttccag catggcaggc
      841 cagaacaggc tgtggaagta aacgatatct ttaccgatga agtggtacag ctcggcggtg
      901 gagtctttct tccagtattc atcgaagctt acgctgtcgc cgcgcttgtc gcacagattc
      961 ttgaaagaac ccatgtagcc aatcggtgcg tccagccaga cgtagaaata tttgcccggt
     1021 gcgttcggaa tttcaaaacc aaagtatggc gcgtcgcggg agatatccca ctgttgcagg
     1081 ccggattcaa accactcctg cattttgttt gccacctgct cctgcaacgc accgctgcgg
     1141 gtccatgcct gcagcatttc gctgaaagag ggcagatcaa agaagaagtg ttcagaatca
     1201 cgcatcactg gtgtagcacc agaaaccacc gatttcggct cgatcaattc agtcgggctg
     1261 taggtcgcgc cgcagacttc gcagttatcg ccgtattgat ccggggattt acatttcggg
     1321 caggtgcctt tcacaaaacg gtccggcagg aacatgcctt tttccggatc gtacagctga
     1381 gagatggtgc ggtttttaat aaaaccgttt tctttcaggc gagagtagat aagttctgac
     1441 aactggcggt tctcttcgct gtgcgtcgag tgatagttgt catagctgat gttaaagcct
     1501 gcgaaatcag tctgatgctc ctgactcatt tcgccaatca tctgctccgg ggtgataccg
     1561 agctgctgag ctttcagcat gatcggtgta ccgtgggcat cgtcggcgca gatgaagttg
     1621 acctcgtggc cgcgcattcg ctggtaacgg acccagacat cagcctggat gtgctccagc
     1681 atatggccga ggtggattga gccgttagcg tacggcagtg cgcacgtcac cagaattttc
     1741 ttcgcgactt gagtcatagt aggcattact tcttaatagt gaaaaggggt tagatagtac
     1801 caaatgggaa aatgttaagt aagtacttct ctatattctt caggtaaact atcagctgat
     1861 ggtttatcta tacaaaaaag gagtcgggat gaacgaacaa tcccaggcca aatcaccaga
     1921 agccttgcgc gcaatggtcg ccgggacact ggccaatttt cagcacccaa ccctgaagca
     1981 taacctcacc acgctgaaag cgttgcacca tgtcgcctgg atggacgaca cgttgcatgt
     2041 ggaactggtt atgccgtttg tctggcatag cgcgttcgaa gagttaaaag agcaatgtag
     2101 cgccgaactg ctgcgcatca ccggggcgaa agctatcgac tggaaactgt cgcataacat
     2161 agccactctg aaacgcgtga agaatcagcc gggaattaac ggtgtgaaga acattatcgc
     2221 ggtgagttcc ggtaaaggcg gcgtgggtaa atcgtccacg gcggtaaacc tggcgctggc
     2281 actggcggcg gaaggggcga aagtcggcat tctggacgcc gatatctacg gcccatcaat
     2341 tcccaccatg ctgggcgcag aaaatcagcg tccaacttcg ccggacggta cccacatggc
     2401 accgattatg tctcatggcc tggcaaccaa ttcaattggt tatctggtca ctgacgacaa
     2461 tgcgatggtg tggcgtggac cgatggcggg caaggcgtta atgcagatgt tgcaggaaac
     2521 cttgtggccg gatctcgact atctggtgct tgatatgccg ccaggcaccg gtgacattca
     2581 attgacgctg gcgcaaaaca ttccagtaac tggtgcagtc gtggtcacca ccccgcagga
     2641 catcgcgttg atcgatgcga aaaaaggcat tgtgatgttc gagaaagtcg aggttccggt
     2701 gctcggtatt gtcgagaaca tgagtgtgca tatttgcagt aactgtggtc accatgaacc
     2761 gattttcggt accggtggtg cagagaaact ggctgaaaaa taccatacac agctgctggg
     2821 gcagatgccg ttacacatct ccttacgtga ggatctcgat aaaggcacgc caacggttat
     2881 cagccgtccg gagagcgaat ttaccgctat ctaccgccag ctggctgacc gcgttgcagc
     2941 tcagctctac tggcagggtg aagtcattcc tggcgagatt tccttccgcg cggtctaacg
     3001 cctattaacc attctggtta ttcttcatac accgcagaga tgttatattg atgcggtgta
     3061 tttgtttttg ccgcattaat gaacacttat ttaatactgc gaatattctg cggaccttcg
     3121 tccgaaactc tacaaagtcg agtgatcgta caggcaatgc ttcattatca tccttcgtgc
     3181 tgtgtagtgg atgtacagct atagtttgtt atgcactcat tctatccagg cataaccttt
     3241 actacaggga taacgttcaa tgaataagta ttggctgtca ggaattattt tt
//
LOCUS       DAJTYL010000222         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYL010000222 DAJTYL010000000
VERSION     DAJTYL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.224"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..218
                     /locus_tag="OO572_003936"
     CDS             <1..218
                     /locus_tag="OO572_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6662737.1"
                     /translation="DNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTL
                     LWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            375..1124
                     /locus_tag="OO572_003937"
     CDS             375..1124
                     /locus_tag="OO572_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6662738.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1146..1712
                     /gene="yieF"
                     /locus_tag="OO572_003938"
                     /gene_synonym="chrR"
     CDS             1146..1712
                     /gene="yieF"
                     /locus_tag="OO572_003938"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6662739.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1766..3103)
                     /gene="adeP"
                     /locus_tag="OO572_003939"
     CDS             complement(1766..3103)
                     /gene="adeP"
                     /locus_tag="OO572_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6662740.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT          903 a          856 c          795 g          704 t
ORIGIN      
        1 ccgacaatct gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc
       61 aactgccgtt ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag
      121 ttccttttac cctgctgtgg cataaacgga acagccataa tccgaagatc gtctggttac
      181 gggaaaccat taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc
      241 caatttcaca ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc
      301 gctttagtct gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa
      361 gcatggagcg aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga
      421 tttctgttcg tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc
      481 aaagccgttt tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca
      541 ttggcgaatt gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa
      601 atttgccttc gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg
      661 aaggtgaatg tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac
      721 acgcgcccga taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg
      781 atcctaacga agcgcgggcg cagctcatta cgctgcgccg aagcgtgcta aaactaaccg
      841 gtgatgtttt gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga
      901 aatgtgctca tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt
      961 ccgtggcggt cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca
     1021 gctggaaaag cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc
     1081 gttttgccca gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag
     1141 tcattatgtc tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat
     1201 ttaatggcat ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg
     1261 cgttaccttc cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt
     1321 ttccagcaac ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg
     1381 tcacgccgga atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt
     1441 cccgcctgcc ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg
     1501 gcgtgattgg cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg
     1561 caatggtgat gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc
     1621 aaaccggaga agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat
     1681 ttggtgagtt tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg
     1741 gcgggttttt aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa
     1801 acagcagcgc aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct
     1861 tcatcacgca gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg
     1921 gcatcatcac ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc
     1981 gagcaagact tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagt
     2041 ctggcaccat tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa
     2101 ccactgccgt cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag
     2161 ccgtaacgga agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca
     2221 gcgcctgctt catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca
     2281 cgccaatcag cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc
     2341 ctgccagccc gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc
     2401 tcggcggcgc agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg
     2461 tcgtcaccac gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa
     2521 tgatgaaaaa gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca
     2581 ccagcgtttc cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa
     2641 acaggccgat accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc
     2701 agtaacgaac gcggaaaatg gtcaaaagaa gcagtccgac agcgccccag aagattgcgc
     2761 ccataccgat ctgccacggc agccccattg cctggacaac cacaaaagcg aaaaaggcgt
     2821 taagccccat cgcaggagcc agcgccaccg gaaggtttgc aaacaatccc atcataatgc
     2881 taccgaaagc agcaatcaga caggttgtta cgaagacggc actggtgtcc atgccagcaa
     2941 cgcccagaat ttgtgggtta acaaatacga tgtagaccat cgtcaggaag gtggtgaacc
     3001 cggcaatcac ttcagttcgt gccgtagtac catgttcgcg cagtttaaaa acacgttcca
     3061 gcatcctctg accagaagtc tgggtggtat gttgatgact cattatctat ttccgaacaa
     3121 ggagggaaaa tccgtcgcta tcgtatacca aaatgcgaca ataggcgcgt ttgtgagaga
     3181 cttttttatt ggatttactt atacggcaac gattgcgttg cgcaaatcgg ctctacgaaa
     3241 acgttaaact gattaaaa
//
LOCUS       DAJTYL010000223         3202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYL010000223 DAJTYL010000000
VERSION     DAJTYL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.227"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..179
                     /locus_tag="OO572_003940"
     CDS             <1..179
                     /locus_tag="OO572_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6662741.1"
                     /translation="FIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIAL
                     YLAFVKRLGAFDAL"
     gene            169..963
                     /locus_tag="OO572_003941"
     CDS             169..963
                     /locus_tag="OO572_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6662742.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            985..2409
                     /gene="patD"
                     /locus_tag="OO572_003942"
     CDS             985..2409
                     /gene="patD"
                     /locus_tag="OO572_003942"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6662743.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            2619..>3202
                     /locus_tag="OO572_003943"
     CDS             2619..>3202
                     /locus_tag="OO572_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6662744.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVA"
BASE COUNT          782 a          677 c          884 g          859 t
ORIGIN      
        1 attttatcgt cccgcagctg gttggtcctc caggatattt tatcggcaat atggtttatt
       61 cccagcaggg ggcgattggc aatatgccga tggcggcggc attcaccctg gtgccgatta
      121 ttctcatcgc actgtacctg gcgttcgtga aacgtctggg agcgttcgat gcactctgaa
      181 cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg ttgttttcct acattttccc
      241 atcctgataa tcgccgccta tgcgtttaac actgaagatg cggcgtttag ttttccaccg
      301 cagggcctga cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat tcttgatgcc
      361 gtgacgctgt cacttaaagt ggcggcgctg gcgacattaa ttgcgctggt gttagggacg
      421 ctggcagctg ccgcgctgtg gagacgagac tttttcggca aaaacgccat ttcgctgtta
      481 ttgctgctgc ccattgcgct gccgggcatt gtcactggtc tggcgctatt aaccgccttt
      541 aaaaccatca atctggagcc gggatttttc accatcgtgg tcggtcatgc gactttttgc
      601 gtagtggtgg tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcgtg gagtctggtt
      661 gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct tccgctacgt agtgttgccg
      721 aatctcagtt cggcgttact ggcaggagga atgctggcgt ttgccttgtc gttcgatgaa
      781 atcatcgtta cgacctttac ggcaggtcat gaacgaacgt taccgttgtg gttgctcaat
      841 cagcttgggc gaccgcgtga tgtaccggta accaacgtgg tggcactgct ggttatgttg
      901 gtaacaacct tgccgatcct gggggcctgg tggctaaccc gcgaaggcga caatggtcaa
      961 taaccactga tacaggaata agctatgcaa cataagttac tgattaacgg agaacgggtt
     1021 agcggcgaag gggaaaaaca gcctgtctat aatccggcaa cgggggacgt tttactggag
     1081 attgccgagg tatccgcaga gcaggtcgat gctgctgtgc gtgcggcaga tgcagcattt
     1141 gctgaatggg ggcaaaccac accgaaagcg cgtgcggaat gcctgctgaa actggcggat
     1201 gttatcgaag aaaatggtca ggtttttgcc gaactggaat cccgtaattg tggcaaaccg
     1261 ctgcatagtg cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg ctttttcgcg
     1321 ggtgcggcgc gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg tcatacttcg
     1381 atgatccgtc gcgatccgtt gggggtcgtg gcttctatcg caccgtggaa ttatccgctg
     1441 atgatggccg cgtggaaact tgctccggcg ctggcggcag ggaactgcgt agtgcttaaa
     1501 ccatcagaaa ttaccccgct gaccgcgttg aagttggcag agctggcgaa agatatcttc
     1561 ccggcaggcg tgattaacat actgtttggc agaggcaaaa cggtgggtga tccgctgacc
     1621 ggtcatccca aagtgcggat ggtgtcgctg acgggctcta tcgccaccgg cgagcacatc
     1681 atcagccata ccgcgtcgtc cattaagcgt actcatatgg aacttggtgg caaagcgcca
     1741 gtgattgttt ttgatgatgc ggatattgaa gcagtggtcg aaggtgtacg tacatttggc
     1801 tattacaatg ctggacagga ttgtactgcg gcttgtcgga tctacgcgca aaaaggcatt
     1861 tacgatacgc tggtggaaaa actgggtgct gcggtggcaa cgttaaaatc tggtgcgccg
     1921 gatgacgagt ctacggagct tggaccttta agctcgctgg cgcatctcga acgcgtcagc
     1981 aaggcagtag aagaggcgaa agcgacaggg cacatcaaag tgatcactgg cggtgaaaag
     2041 cgcaagggta atggctatta ctatgcgccg acgctgctgg ctggcgcatt acaggacgat
     2101 gccatcgtgc aaaaagaggt atttggtcca gtagtgagtg ttacgccctt cgacaacgaa
     2161 gaacaggtgg tgaactgggc gaatgacagc cagtacggac ttgcatcttc ggtatggacg
     2221 aaagatgtgg gcagggcgca tcgcgtcagc gcacggctgc aatatggttg tacctgggtc
     2281 aatacccatt tcatgctggt aagtgaaatg ccgcacggtg ggcagaaact ttctggttac
     2341 ggcaaggata tgtcacttta tgggctggag gattacaccg tcgtccgcca cgtcatggtt
     2401 aaacattaag ttagatgtta gtggctgtta taggacggcc actatcgttt taggttctga
     2461 gtttaatggc ggttaagtat tagaatgatt acattaataa ctggcgtaaa aattaggcta
     2521 aatgtttgat ggcgattttt catcctgtca cagttgatta gcataaggct ttagagtatc
     2581 gttattaaac taatacttta taagtagaag tattaacaat gaaagttcaa ttgtacaaat
     2641 tcacggaaga taaaaataaa acccttacct tcagatggac gaaaaaacac tttgagtttt
     2701 gtatggataa taaaatcttt ttaaatcata aggggaagaa gtcgtataaa gaaaggaatt
     2761 tatttctttt ctcaaaaggt gacaagataa caattgaaga taatgttatt gctgaagaat
     2821 attcaacaat gcctgtgaaa aatttctcca gtgtcggcgc tttttcattt ccgacctgcc
     2881 atttttcagg gaatatacgt ataggtcgat tttgtagtat tgcatcaaat gtaaaaataa
     2941 tgggtggaaa tcatcctttg aatcgtttta ctactcacat gatgacttat aacggtgaat
     3001 ttgataagtt tgctatgagt gaatttgaac gtagctggac attgaaacca ttcattacca
     3061 aacctgaaaa tccgattata ggaaatgatg tctggatagg aaatgatgtt gtattgaaag
     3121 gtggcatcgc aataggtgat ggtgctgtta ttgctgccaa ttccgttgtt accaaagatg
     3181 tgcccccata tgccatagtt gc
//
LOCUS       DAJTYL010000224         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYL010000224 DAJTYL010000000
VERSION     DAJTYL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.228"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            102..3005
                     /locus_tag="OO572_003944"
     rRNA            102..3005
                     /locus_tag="OO572_003944"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO572_003945"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO572_003945"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          702 c          992 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DAJTYL010000225         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYL010000225 DAJTYL010000000
VERSION     DAJTYL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.229"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO572_003946"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO572_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6662745.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO572_003947"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO572_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTYL010000226         3168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYL010000226 DAJTYL010000000
VERSION     DAJTYL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.230"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..1858
                     /gene="betT"
                     /locus_tag="OO572_003948"
     CDS             <1..1858
                     /gene="betT"
                     /locus_tag="OO572_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6662746.1"
                     /translation="LQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQP
                     DFSYHSWFAMLFSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWG
                     LHAWAIYAIVALILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFG
                     VATSLGYGVLQVNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSEL
                     NLGLALLLLALVLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWT
                     LLYWGWWLSWSPFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIM
                     NQGATDLANTVQQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTL
                     ASGGVANTPVWQRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGL
                     LKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAM
                     TLVQEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDND
                     EQQTEQHRYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            1921..>3168
                     /locus_tag="OO572_003949"
     CDS             1921..>3168
                     /locus_tag="OO572_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6662747.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLF"
BASE COUNT          736 a          731 c          816 g          885 t
ORIGIN      
        1 acttcagcaa caaatcttca cgaatgccag ctggttctac atccttgctg tggccctgat
       61 tttactgagt gtcacgttcc ttggactctc acgctacggt gatatcaagc tgggcccgga
      121 ccatgcgcag cctgatttca gctaccactc ctggtttgcg atgctttttt cggcagggat
      181 ggggatcggc ctgatgttct ttggcgttgc cgaacctgta atgcattatc tttcgccacc
      241 cgttggcact ccagaaaccg ttgcggcagc taaggaagca atgcgtctga cctttttcca
      301 ctggggactg cacgcatggg caatttatgc cattgtggcg ctgattctgg cgttcttcag
      361 ttaccgtcac ggtctgcctt taactctgcg ctccgcactc tatcccatta ttggcgatcg
      421 catatacgga cctgtaggac atgcggttga tattttcgct gttataggca cggtctttgg
      481 cgttgcgaca tcactgggtt acggtgtttt gcaggtgaat gccggtttga accatctttt
      541 cggggtgccc atcaatgaaa cggtgcaggt aattctgatc gtggtcatca cggggttagc
      601 gacgatttca gtggtgtccg gtctggataa gggaatacgt atcctgtctg aactcaatct
      661 gggtctggct ttgttgctcc tggcgctggt cctgtgtctg ggaccaaccg tgcttctgct
      721 gaagtcattt gtggaaaata cgggcggtta tctttcggaa ctggtgagta aaacgttcaa
      781 cctttacgcg tatgagccca agtcgagcaa ctggctgggg ggctggacat tactgtactg
      841 gggatggtgg ctttcatggt cgccgtttgt ggggatgttc atcgcacggg tctcccgcgg
      901 gcgaaccatt cgcgagtttg tcaccggcgt gctgtttgtt cccgcgggtt ttacgctaat
      961 gtggatgacg gtgtttggta acagcgcgat ctatctcatt atgaaccagg gggccacaga
     1021 cctcgccaat actgttcagc aggatgtggc gctggccctg tttaatttcc tggagcattt
     1081 cccgttctct tctgtgctgt cattcattgc aatggcgatg gtcatcgtct tctttgtaac
     1141 gtctgctgat tcgggggcaa tggttgtgga tactctggca tcaggtggag tggcaaacac
     1201 acccgtctgg cagcgaatat tctgggcctc gctcatgggc attgttgcaa ttgcgcttct
     1261 ccttgccgga gggctaagtg cgctgcaaac ggtgacaata gcgagtgcat tgcccttctc
     1321 agtgatctta ctaatatcta tatacggact tttaaaagct ttgcgccggg atttgaccaa
     1381 gcgtgaaagc ctgagcatgg cgacaattgc tcctacggct gcacgtaacc caattccttg
     1441 gcagagaagg ttacgcaata tcgcgtatct gccgaagcga tctcttgtga aacgttttat
     1501 ggacgacgtt atccagcctg ccatgacgct ggttcaggag gaactgaaca agcaggggac
     1561 gataagccac attagtgatg cagtcgacga tcgtattcgt cttgaagtcg atttgggcaa
     1621 cgagctgaat tttatatatg aagtgaggct tcgcgggtat atctcaccga ccttcgcgct
     1681 cgccgcaatg gataatgatg agcagcagac tgaacaacat cgatattatc gcgctgaggt
     1741 ttatctcaaa gaaggcggtc aaaattatga tgtgatgggc tggaaccagg aacagctgat
     1801 taatgacata ctggaccagt acgaaaaaca cctgcacttc ctgcacctgg ttcgttaata
     1861 gcaacatgcc gtcctggggg cggcaattat tatcccggcc gcaatatgag ggaatgcaga
     1921 atgatttcac gctggaaatg gatgctgaag cagacaatta aaaaactatg gttcagggca
     1981 acgttattcg caattgtcgc gataataacg gcccttttat caattctttt taaatcaatg
     2041 atacctgagt cggtttccgt gaaggttggt gcggaagcag tcgataacat tctgaacata
     2101 ctggcatcga gtatgctggc agtgaccaca ttttcgctga gtatcatggt cacagcctac
     2161 ggttcagcca ctactaatgt gactcccaga gctacgcgtt tagttgttga agacgtcacc
     2221 acacaaaatg tactggccac cttcatcggt tcttttctct tcagtctggt agggattatt
     2281 gccctcaata tgggagctta tggagaaagg gggagagtca ttttattcat tgtcacactg
     2341 gttgtcattg ccttaatcct catcacattg cttcgctgga tacagcattt gacctctctg
     2401 gggagggttg gtgagacaac ggcacaagta gaacaggcgg ccatcgaaac atttattgcg
     2461 agagcaagaa atccctgtct cggtggatat ccatggcttg agaacaatga acagccgaaa
     2521 ggaacggttg cagtttatcc gaagaagatt ggctatgttg aatatattga tatggtgaaa
     2581 cttagcaagc tgctgaccaa tgatccccgt catgtatacc tcgtggcgca gccaggcagt
     2641 ttcatacatc cgtccatgcc agttttgtac ctgagtcagg gccaggagtc gtcaatcaac
     2701 accgatttac ttgagacgat tattgtctcg gatgcacgtt catttgctca ggatcctcga
     2761 ttttgtctta gcgtcatggc cgaaatagcc tgccgggccc tttcccctgc agtgaacgat
     2821 cctggaaccg ccattgatgt cattggcaga ggtgttcgta tactttccac ttacgcgcag
     2881 aataaatctg atgaaataga agtgaaatat ccttcagtac atgttgcacc acttcagaat
     2941 aacgatctac tggaagactt tttctcacct gtcgcgcgcg atggtgccag tatgagggag
     3001 attcagataa gagtccttaa aggactgtcg atgctgagta aaggttggcc tgagatattt
     3061 gctgaagccg cacataccct ggcatttgaa acattagagc atgcaactcg tgctgaccat
     3121 atagattctg atagatatct aataaaatca acttattata atttattt
//
LOCUS       DAJTYL010000227         3143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYL010000227 DAJTYL010000000
VERSION     DAJTYL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.232"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..442
                     /locus_tag="OO572_003950"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO572_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO572_003951"
     CDS             1026..1742
                     /locus_tag="OO572_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662748.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO572_003952"
     CDS             2294..2698
                     /locus_tag="OO572_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662749.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3143
                     /locus_tag="OO572_003953"
                     /pseudo
     CDS             2761..3143
                     /locus_tag="OO572_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          885 a          641 c          711 g          906 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa aca
//
LOCUS       DAJTYL010000228         3120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYL010000228 DAJTYL010000000
VERSION     DAJTYL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.233"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(331..594)
                     /gene="rpsT"
                     /locus_tag="OO572_003954"
     CDS             complement(331..594)
                     /gene="rpsT"
                     /locus_tag="OO572_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6662750.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            697..915
                     /locus_tag="OO572_003955"
     CDS             697..915
                     /locus_tag="OO572_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6662751.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            923..1864
                     /gene="ribF"
                     /locus_tag="OO572_003956"
     CDS             923..1864
                     /gene="ribF"
                     /locus_tag="OO572_003956"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6662752.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1907..>3120
                     /locus_tag="OO572_003957"
     CDS             1907..>3120
                     /locus_tag="OO572_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCR6662753.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYP"
BASE COUNT          737 a          755 c          846 g          782 t
ORIGIN      
        1 aaggtctaat gaggagatat ttatgaatgg ttaaagtata taggaattta attcaaaaat
       61 agagtggtct taagtttaat acctcaatgt gtatcacaat atccatattc tttgtggggg
      121 agtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt tctacgattc
      181 ttaagccacg aagagttcag atagtacaac ggcatgtctc ttttgactat ctggcaaccg
      241 gcagtgtgtt ctctcacgca tcacaaaagc agcaggcata aaaaaacccg cttgcgcggg
      301 ctttttcaca aagcttcagc aaattggcga ttaagccagt ttgttgatct gtgcagtcag
      361 gttagcctta tgacgtgcag ctttgttttt gtggatcaga cctttagcag cctgacggtc
      421 cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat
      481 agctgcgtat actttcttga tgaaagtacg catcatagag cgacggcttg cgttgtgctt
      541 acgagccttt tcagactgaa tggcgcgctt cttagctgat ttgatattag ccaaggtcca
      601 actcccaaat gtgttctata tggacaattc aaaggccgag gaatatgccc ttttagcctt
      661 cttttgtcaa tggatttgtg caaataagcg ccgttaatgt gccggcactc gttacgtagt
      721 gatggcgcag gattctacca gcttgcgggg tgtgaataca gcttttccgc gataaaaatt
      781 gcagcaggcg gtcagtttct tcccgtgatt tgctccatgg caatgaaaag ccacttcttt
      841 ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat actcggacga
      901 ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc tcagccaggc
      961 cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc gcggtcatcg
     1021 cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat
     1081 gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg caagactgac
     1141 ccggctgcgg gaaaaactgc gttaccttgc agagtgtggc gttgattacg tgctgtgcgt
     1201 gcgtttcgac aggcgttttg cggcgttaac cgcgcaaaat ttcatcagcg atcttttggt
     1261 gaagcatttg cgcgtaaaat ttcttgccgt aggtgatgat ttccgctttg gcgctggtcg
     1321 tgaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg atatcaccag
     1381 tacgcaaact ttttgcgaag gtggtgtgcg tatcagcagc accgccgtgc gtcaggcgct
     1441 ggctgatgac aatctggctc tggcagaaag tttactgggg cacccgtttg ccatctccgg
     1501 gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg cgaatgtacc
     1561 gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc tgggcctcgg
     1621 tgaaaagccg ttacccggcg tggcaaacat cggaacacgc ccaacggttg ccggtattcg
     1681 ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc gccatataca
     1741 agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcgtcgctgg acgaactgaa
     1801 agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa caaaaccggc
     1861 ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga gtgactataa
     1921 atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg ccaagcgcga
     1981 accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcatcc gtgcggctaa
     2041 aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg gcagcattca
     2101 tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca aagggctttc
     2161 cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga tcgagctgaa
     2221 agtagagcaa gaatacggta agccgggtga gaaattcacc gccgccgagt tccgcgccaa
     2281 gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta tccgtctggg
     2341 cgtgctgggc gactggtcgc acccgtatct gactatggac ttcaaaactg aagccaacat
     2401 catccgcgcg ctgggcaaaa tcatcggcaa tggtcacctg cacaaaggcg cgaagccggt
     2461 gcactggtgc gtagactgcc gttctgcact ggcagaagcg gaagttgagt attacgacaa
     2521 aacttctccg tccattgacg tcgctttcca ggcggtcgat caggatgcgc tgaaagcgaa
     2581 atttggcgta agcaacgtta acggcccaat ctcgctggtg atctggacca ctacgccgtg
     2641 gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg cgctggtgca
     2701 gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa tgcagcgtat
     2761 cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcagagcttg agctgctgcg
     2821 ctttacccat ccgtttatgg gcttcgacgt tccggcaatc ctcggcgatc acgttaccct
     2881 ggatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg actatgtgat
     2941 cggtcagaaa tacggcctgg aaaccgctaa cccggttggc ccggacggca cttatctgcc
     3001 gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca tcgtcgttgc
     3061 gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca gctatccgtg
//
LOCUS       DAJTYL010000229         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYL010000229 DAJTYL010000000
VERSION     DAJTYL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.234"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(361..1056)
                     /locus_tag="OO572_003958"
     CDS             complement(361..1056)
                     /locus_tag="OO572_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6662754.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1049..2476)
                     /locus_tag="OO572_003959"
     CDS             complement(1049..2476)
                     /locus_tag="OO572_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6662755.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2487..>3069)
                     /locus_tag="OO572_003960"
     CDS             complement(2487..>3069)
                     /locus_tag="OO572_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6662756.1"
                     /translation="INSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTY
                     LADEPEWASRAEKVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKD
                     EPLTLLKNVRGLELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLI
                     GADVSCLLNISGRLQREGQKVKVMHIAEVLMSR"
BASE COUNT          751 a          859 c          664 g          795 t
ORIGIN      
        1 taaataccct gctgccattt gcctggctct gggatttttt ctaagcattg taatttctgt
       61 aatgttttgc cttgtcaaaa aaatggtaga tgaataccaa caaaactctg ggcagtaata
      121 aacagcattc tgatagagag tgcctgtata aagctgaagt gacaatgacc gggaagggaa
      181 cccgttttca ggaatggatg cctgtctttt tcactgtaaa tatcctggca ttactaatgc
      241 ctggatattt acagaagagc agcataacca tgacggaacg gggttaagtc aggttgtcct
      301 taacgctatt ttcaacctcg tatgccttct tcaggtttat gttcagatct catatctctc
      361 tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc cgacgacttt
      421 gataagctca atatccgccg ttgagctggg accactgatg atgttaatac atgacggcat
      481 tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta cacgcggcag
      541 gattgtgctt ttacgcagga taaaaagaga agattccggg agcaggctca atgaacgccc
      601 gcgctcggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat attcagcata
      661 cacgacaccc actttagcct gctctgcctg cgagatattc tcggcacctt tcgccggatc
      721 ccaaacaacg gcattgcatt cctgctgcaa acgttcacta atccccaatt cctccagcct
      781 cgtgtcaccg ctaatcacga ccgactgatc tcccagctct ttacacagac gtattgcagc
      841 ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc aacataacat cgctggcaaa
      901 ctgaataaac gcgtcacagc gctgctgttg gttaagttgg gtaagccgct cgttagcata
      961 gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg
     1021 agcaacgtta ttcaaaaatt cgctccgatt atccattctt tttctcctgc gcctgatgtt
     1081 tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt gcttccatcc
     1141 agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac cagcttgccg
     1201 catgagcgcc ggccatcatc ccgactttcc acaatcctgg atgactattg gcataagcga
     1261 acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc atcacccgac
     1321 gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgctg tcacaagcag
     1381 tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca agtagcggag
     1441 aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg ccaatatggc
     1501 gatatgccgg acaagtattc atacaagccc cgcagcgaat acagcgcagc acatcccgaa
     1561 attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga aactcttcag
     1621 gaccatcaac gtgccccgct tcgcgcggcc ctgtcagcca ggtgttgtat cccgtcaaac
     1681 gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg gcaaacgtgg
     1741 gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg gtacacattc
     1801 gcgcattacc ttcattggtc accaggcata ccgaaccggt ctctgccacc gcgaaattac
     1861 agccggtaat acctatttca gcactgagga aatcttcacg gattttttgc cggatgaata
     1921 aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt agcactcgac
     1981 ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagatggc ggatcttgat
     2041 ccagctggag aatatattca cccagatcgg tttcaatcac ctgaatgcca gcatcctgca
     2101 acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc accttccggg
     2161 catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa
     2221 aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga taagcgtcga
     2281 gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc tcccagtgcc
     2341 ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg cttgccaccg
     2401 ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa tctgtattac
     2461 tggttttgat cgacatcttt atatcctcag cggctcatca acacttcagc aatatgcatc
     2521 actttgactt tctgcccttc ccgttgtaat cgcccactga tgttcagcag gcaactcacg
     2581 tcagcaccaa ttaaatactc agggcggact tccatcaggt gcacaacctt ttctttcacc
     2641 atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc gcagcaggta
     2701 tcctgttcag caaaggtcaa cagctccagt ccacgcacat ttttcagcag cgtaagtggc
     2761 tcgtccttca ctcccagctt acgggccagg ctacaagatg ggtgatacac agctctccct
     2821 tgcaaactgg cacctacatc gactacccct aatttattaa caataaaaga ggtgagatcc
     2881 tgcatacgcg cggcaacctt ttcggcacgt gatgcccatt caggttcatc cgccagatac
     2941 gtcgggtaac tttttatggc ataggtgcaa gagccagccg gtgaaataat gggatcgtcg
     3001 ttatcctcca gtgcggcgat cagatttttc atccctggaa tcgcttcttt gatataaccg
     3061 ctattgatc
//
LOCUS       DAJTYL010000230         3044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYL010000230 DAJTYL010000000
VERSION     DAJTYL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.235"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..556)
                     /gene="tetR(B)"
                     /locus_tag="OO572_003961"
     CDS             complement(<1..556)
                     /gene="tetR(B)"
                     /locus_tag="OO572_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6662757.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPA"
     gene            638..1843
                     /gene="tet(B)"
                     /locus_tag="OO572_003962"
     CDS             638..1843
                     /gene="tet(B)"
                     /locus_tag="OO572_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6662758.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1956..2549)
                     /gene="tetC"
                     /locus_tag="OO572_003963"
     CDS             complement(1956..2549)
                     /gene="tetC"
                     /locus_tag="OO572_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6662759.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2682..>3044
                     /locus_tag="OO572_003964"
     CDS             2682..>3044
                     /locus_tag="OO572_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6662760.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEA"
BASE COUNT          824 a          555 c          623 g         1042 t
ORIGIN      
        1 aggctggctc tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca
       61 tactatcagt agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca
      121 atacgcaacc taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg
      181 aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg
      241 taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa
      301 aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt
      361 gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt
      421 ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg
      481 ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt
      541 tatctaatct agacatcatt aattcctaat ttttgttgac actctatcat tgatagagtt
      601 attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga caaagatcgc
      661 attggtaatt acgttactcg atgccatggg gattggcctt atcatgccag tcttgccaac
      721 gttattacgt gaatttattg cttcggaaga tatcgctaac cactttggcg tattgcttgc
      781 actttatgcg ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt
      841 tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca tcgctggatt acttattgct
      901 ggctttttca agtgcgcttt ggatgctgta tttaggccgt ttgctttcag ggatcacagg
      961 agctactggg gctgtcgcgg catcggtcat tgccgatacc acctcagctt ctcaacgcgt
     1021 gaagtggttc ggttggttag gggcaagttt tgggcttggt ttaatagcgg ggcctattat
     1081 tggtggtttt gcaggagaga tttcaccgca tagtcccttt tttatcgctg cgttgctaaa
     1141 tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa accaaaaata cacgtgataa
     1201 tacagatacc gaagtagggg ttgagacgca atcgaattcg gtatacatca ctttatttaa
     1261 aacgatgccc attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc
     1321 aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt
     1381 ttcattagcg ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat
     1441 agccactaaa tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc
     1501 atttgccttt ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt
     1561 ggctggtggt gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag
     1621 tcatcagcaa ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat
     1681 tggcccatta ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat
     1741 ttggattatt ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt
     1801 aacccctcaa gctcagggga gtaaacagga gacaagtgct tagttatttc gtcaccaaat
     1861 gatgttattc cgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttacgat
     1921 aaagaagatt tagcttcaaa taaaacctat ctattttatt tatctttcaa gctcaataaa
     1981 aagccgcggt aaatagcaat aaattggcct tttttatcgg caagctcttt taggtttttc
     2041 gcatgtattg cgatatgcat aaaccagcca ttgagtaagt ttttaagcac atcatcatca
     2101 taagctttaa gttggttctc ttggatcaat ttgctgacaa tggcgtttac cttaccagta
     2161 atgtattcaa ggctaatttt ttcaagttca ttccaaccaa tgataggcat cacttcttgg
     2221 atagggataa ggtttttatt attatcaata atataatcaa gataatgttc aaatatactt
     2281 tctaaggcag accaaccatt tgttaaatca gtttttgttg tgatgtaggc atcaatcata
     2341 attaattgct gcttataaca ggcactgagt aattgttttt tatttttaaa gtgatgataa
     2401 aaggcacctt tggtcaccaa cgcttttccc gagatctcat ctattgaaac agcttgatag
     2461 cctttttcaa caaacaatat tcgtgctgag ttaaccagtg attgataggt actcttaaaa
     2521 ttttcttgtt gatgattttt attttccatg atagatttaa aataacatac cgtcagtatg
     2581 tttatggtat catgatgatg tggtcgtgac aatcttaaga acatttaggt tattttatgt
     2641 atattgaaca gcattctcgc tatcaaaata aagctaataa catccaatta agatatgatg
     2701 ataagcagtt tcatacaacg gttatcaaag atgttctatt atggattgaa cataatttag
     2761 atcagtcttt actgcttgat gatgtggcga ataaagcggg ttataccaag tggtattttc
     2821 agcggctgtt caaaaaagta acaggggtca cactggctag ctatattcgt gctcgtcgtt
     2881 tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac tatccttgag atcgcattaa
     2941 aatatcaatt tgattcccaa caatctttta cacgtcgatt taagtacatt tttaaggtta
     3001 caccaagtta ttatcggcgt aataaattat gggaattgga ggca
//
LOCUS       DAJTYL010000231         3035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTYL010000231 DAJTYL010000000
VERSION     DAJTYL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.236"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..642)
                     /gene="ipaH9.8"
                     /locus_tag="OO572_003965"
     CDS             complement(<1..642)
                     /gene="ipaH9.8"
                     /locus_tag="OO572_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6662761.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDN"
     gene            complement(904..999)
                     /locus_tag="OO572_003966"
                     /pseudo
     CDS             complement(904..999)
                     /locus_tag="OO572_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1035..1340)
                     /gene="ccdB"
                     /locus_tag="OO572_003967"
     CDS             complement(1035..1340)
                     /gene="ccdB"
                     /locus_tag="OO572_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6662762.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1342..1560)
                     /gene="ccdA"
                     /locus_tag="OO572_003968"
     CDS             complement(1342..1560)
                     /gene="ccdA"
                     /locus_tag="OO572_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6662763.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2096..3035)
                     /locus_tag="OO572_003969"
                     /pseudo
     CDS             complement(2096..3035)
                     /locus_tag="OO572_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          755 a          741 c          727 g          812 t
ORIGIN      
        1 gttatcacta atatgtattg aacattcatt cctcagatta agaatgcttt ccgggatatg
       61 acttatctga tttctatcaa aaaaatactc tctgacaacg ggattatttc cctcagaaaa
      121 tgctggtagc tcagtgagga aattacgggt cgctctgaga tttttaagag cctgtgggag
      181 cgatggtaag gagactattt cgttatagct gatattcata gtcaataacg aatcgggtaa
      241 agctggcagg ttttccagct cattgtgttg tacctgaagt gactccagcg caggaggtag
      301 cacgggcaat tctgataatt tattgctggc ggaatataat ttttttagcg taacaggcag
      361 ttcaggtagg ttagttaatt gattatatga tacattgagc agcgttatct gagctggtaa
      421 gttgtcaggt agcgaggaca gatttaaacg gtccagtcga agttcatcgg aattattgat
      481 aagacattct ttaagtcggg agacagcctc atcacgctct tcaccgggga gcgcttgttt
      541 ttcccattta tcccatgctg aaaagtaatc agcatatgta ccggaaatag tgttataaaa
      601 agaattttgg ggcaatgaaa agttattatt tatcggtaac atgatgggca tccagttaaa
      661 gccggtggac aaatggcttt gttaaaagaa ataagtagga gtttttatga agttcattcc
      721 atgaaatgaa cttcagcact tttttattat aataaggata aacaaaattg gctgtaaaaa
      781 aacgatactg tttcagttaa ccgtgaatgg cctgtttgca ccgtattgtt cttgtttttc
      841 ggttagttaa acatcgtact tcatatttga acggtaagcg gctcgtcaga accgtattga
      901 tatttactga gaggtcagat caactttcca aggcaacaga tcgcgtaccc ggtttgccgg
      961 ccagtcctgg atatgctcaa tgacgtaacg cagccatttt gtcaacgcca cgatttttga
     1021 ccctagcctg acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt
     1081 tcgcggtggc tgagatcagc cacttcttcc ccgataacga agatcggcac actggccata
     1141 tcagtggtca tcatgcgcca gctttcatcc ccgacatgca ccaccgggta aagctcacgg
     1201 gagactttat ctgacagcag gcgggcactg gccaggggga tcaccatccg tcgtccgggc
     1261 gtgtcaataa tgtcactctg tacatccaca aacagacgat aacgactctc tcttttatag
     1321 gcgtaaacct taaactgcat ttcaccagtc cctgttctca tcggcaaaag agccgttcat
     1381 ttcaataaaa cgggcgatct cagccatccc ctgctgattt tcagcctgcc agcgttccgc
     1441 acgcagacgg cgggcctctt tttgcatggc cgtatttacc aggcctgaga tattcacgtt
     1501 tgcggattta agtaactgat agctgtcact gtctatagtg acagtaatac gctgcttcat
     1561 gataagccct caaaaacata cttatgatat gtatatgagt atatattaat ggtacaaacc
     1621 atcaatatcc ataaaaagta caccattaat tcgctataag tttttaatac aattttttct
     1681 gctgcgattt ttttatacta taattagcat acttataaca ccgtaagtaa attacatgaa
     1741 agcaggcaca gcgtgatccg atttttctgc gtataacgat tttccggaag tgtaatacgg
     1801 ttgcgccccg ggccgggcac ccttttctgt tgttgtgtac gggcccacag agaatggtac
     1861 cggaggcggg ctcagaagta ctgagtcggg tgatcgcgtc aggccgcgaa gtactgacac
     1921 gggtattggt gactggtgat gaagtactgc cccgggtatg atttcaggct gattcaggtt
     1981 ctgattctct gtcagtccgc gtggttgctg gcctggctga tttcctgttg tcaggcgttc
     2041 aacaaccgac tgcaggttat agcccggttc acgaacacct gacggcaggt atcactcacc
     2101 cggtgaaggt aaatccctca agaggcagca actctgccag tcgctcctcc ggccattccg
     2161 gcagacgcgt caggacgtcc gtcagccagg catgtggctc cagaccatta cgttttgctg
     2221 tttccagaag gctcattatc tgtgccgccc ggtttccggc catcagtgaa ccggcgaaca
     2281 gccacgcttt ccgtcccata acaaccgggc gaatggcacg ctcgcagata ttgttgtcca
     2341 ggggcacagc tccgtcaccc aggaagcagc tcagttcatc gcgccggttc agaatatagt
     2401 taatcgcctt atttaatggc ccgtcgggcg gacagcacgg ctcctgctct tcaagccagg
     2461 caaacaggtc attcactatc ggccgggagt accgttatcg ccactgccgt attttttcca
     2521 cagggcgctc acggatgagt ttctcaatgc ggtaaagacc cgcgattttt ctcagctcca
     2581 cgcctgcacg gggctccctg ctggccttat acagatttgc gaagccgcgc cctgcgtgac
     2641 tccagcaaca ggctgatgtg atgccggggt gattatccgc cagagatttg tacacgctgt
     2701 atccgtcact caccagcgta ctgccacacc agcactgcag ccaggtctca gggtatctga
     2761 gggcccgtcc ggtctgcgag tcaaaacaga cgaccggcgg accgcttctt tcaccgctga
     2821 cgtaagcaca cagccagccg gagcagggtt taccgccttt ccgggtatca aggagcctca
     2881 gggacgtttc atctgcatga ataacatccc gtgtcagaag ctcatggcgt agcagtttcg
     2941 ccagcggggc cagtgcagct ccggcaacac cgaccatatc cgccattgta ctgacgggga
     3001 gctccacacc cattcgggag aaaatgtgct gctgg
//
LOCUS       DAJTYL010000232         3025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYL010000232 DAJTYL010000000
VERSION     DAJTYL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.237"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..413)
                     /locus_tag="OO572_003970"
     CDS             complement(<1..413)
                     /locus_tag="OO572_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6662764.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSA"
     gene            complement(413..1261)
                     /locus_tag="OO572_003971"
     CDS             complement(413..1261)
                     /locus_tag="OO572_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662765.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(1261..2679)
                     /locus_tag="OO572_003972"
     CDS             complement(1261..2679)
                     /locus_tag="OO572_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6662766.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(2680..>3025)
                     /locus_tag="OO572_003973"
     CDS             complement(2680..>3025)
                     /locus_tag="OO572_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6662767.1"
                     /translation="SPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPD
                     YALEAPAKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNY
                     FHRKEPCDNGGE"
BASE COUNT          715 a          783 c          758 g          769 t
ORIGIN      
        1 gcactgtaaa atgtcacgtc atccacgcct ttgaagtatt tcttgatggt tcctactcgc
       61 ttaaggccaa tcattgcgca gtacatctga ccgtggcgga atttgcgcgc agcgaacgat
      121 gtgacgcact gaacggtggt gttagtcaga atgtatcgcc agaacgccag cccgatttcc
      181 ttgctgaatc cacgaacctc tggcaggtac atggcgtggc aatcaaaggt caaaggctga
      241 atctcctgat agtaaacaat gccgccgaac tgcccgtgca cgttcacctc gaagtaacgg
      301 cattcaggct tgtagtcgta tccatcaccg ttgttgctcc cggcgataat gtcggggtga
      361 tttcccacgg cttctatcag gtcgatgttt cgcgttggtt tgaactgaat catcactgct
      421 ccgcgattat cttgatggtt gtggcagtaa acgccgcacc attcgactga atggttaacg
      481 tactgccatt tgtggcaaga aatccgtctt tatccacgct gaagaatgta gctaacagga
      541 tgttgtcggt tgttgtcgcc gcattacgac tgctgaccag cgtatcagga acagagccgg
      601 aaaaggttag ctgcattgac ctgttggcgg ttccgctggg ccacgtcccg acgatcgaca
      661 gcttgaagaa caaggttttg ttctcgttga acacaaccat cttgttgtta acggtgtcga
      721 agaatggtgc caacgtgccg gatgacggcg tgagcgtttt cagtaggcta acaaggttgg
      781 tcggcgctgt cgggatggtt acagatacgc cagagtaaac aacctctgac ttcttgcgag
      841 tagtggcata ctccagagca tcgatgcgcg tttcatggtc tgaaacctgc gattccagcg
      901 actgaactct ggtatcaagc gacgcaatat cgctttcatt ctgagcgatt cgcgtttcat
      961 gttcctgaag agttgattct gcctggctga ttcgctcctc atgattaaca agcgttgctt
     1021 ccgcagcaga aattcgctgc tcatggtcag cgagaatcac atcctgctca tcgttcctga
     1081 cttgtgcgtc ataagcgccc tgtccggcct cgttggcctt gttggccacg ttaccaacat
     1141 cagtgccctg tgcgataacg taaagcagat acgactgcga gaagatattg cgtggaagga
     1201 ctgatgtgtc gagccgtgta gcctgaatga ttaccggcac attgagattc gaatccgcca
     1261 ttactcaatc cttatctggc agccagacag agtgacaggt gacttcgtga taacgcgcaa
     1321 tttgaagccg acatttttcc tgatgcgccc tactcgcttc cacaaaacgc gtttgtcgta
     1381 aacgaacggt tcattctgct caatcatctg ctcacacccg taattgatgc cgtcagtggt
     1441 tgcagagaga aaaaggcggt cagcatactg cgcaacgcca gttgacgatt caacctcaag
     1501 gtcgaacact ctggcgttat ccgctttgaa cagtggagta aacagcaggt gttcctgttg
     1561 cttgtcgtac tggctgctga tgtcgaattg caatttcccg gtcacggact ccagcttatc
     1621 gccgcacgtt atttgattgc cttcgtaaat gaagtcgata gcgcggtaca catcgtcata
     1681 caggcctgtt ttcagcacac accattgcgg accattggcg cttgaagatg cgtcgtacac
     1741 cagtacatgg cgcggaaggt ggataatcag caactcatgc gcatcaaacc gcagcgattc
     1801 catcacgcca tcagccagtt catcagcagt gtaggagcgg aggattttct caatgctcgc
     1861 gctggcgatt ggtgacacct gaccggagcc gatgatgtat acagacggcg cacctgttgc
     1921 cggattgctg atgaacgcat aagaatcagc gaatggtgtt ttgcagtaag tcccggcaat
     1981 acctttctgc accatcagcg atggctgtgc gacatacaaa gcggcaccaa cggtggttgc
     2041 acctgtcagg gaaaaatatt caatcgtcga tgagccaaag cagacgatga agtctcgcca
     2101 tgtacctatg ccgatgatgc cgtccggctg agactcggca cgatattgtg cactgtagcg
     2161 gtcaggatgc gattcatctt caaggtcagt gataaaccat gaatcagtgc cgtcttttga
     2221 ccacgcataa cgcccacgta agcgcgtaat gtcgcgaact gaacctaact catactgagt
     2281 gaatccactg tctgtaggcc agtttgagac ggttttaacc gtgccatcat agcgatactc
     2341 gaccagttga ccattaacgc ctaccgcctg tgatgtccga ccatgcgcca ttgatacgcg
     2401 accacttccg gcaacatcac cgacctcgct ttctccctta tacaacttgc caccgcaaac
     2461 gcgataaaca gcattctgcg ccatgttgta ctcgacaccg cgcgatacac cattcacatc
     2521 agaacgtttg gcaatgcccg ggaatgagcg aagatatccg ctgctgttga gtatttcttt
     2581 gggtgtagcc agcatattca ctggcagata gtcgatgtag tcggcgtttc ggaaatcttt
     2641 gccgacacct ttcataagcg gaagttgctg aatcggcatt tattcgcccc cgttatcgca
     2701 aggttccttc cggtggaagt aattccaacc attccacttc gccaactggt taccgctacc
     2761 aacaggcata cggtttggat aaccggactt acatttagcg gcttttgctc tgtccattgc
     2821 agacagtttg acgagtcgct ctttcccgta tctggcagtg gttataagtt ttgctggcgc
     2881 ttccagcgca taatctggag caatgcggca ggcaaggttg aaaatgacgg cattgatagc
     2941 gttatttgat aaaccgtgct catcgcccgg ttctggagcg acatctgcat cagcgaaaat
     3001 gtagccaacg ttgataccag gtgac
//
LOCUS       DAJTYL010000233         2990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYL010000233 DAJTYL010000000
VERSION     DAJTYL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.238"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..125
                     /locus_tag="OO572_003974"
     CDS             <1..125
                     /locus_tag="OO572_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6662768.1"
                     /translation="WNLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQSP"
     gene            complement(406..514)
                     /locus_tag="OO572_003975"
                     /pseudo
     CDS             complement(406..514)
                     /locus_tag="OO572_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1037..1113)
                     /locus_tag="OO572_003976"
     tRNA            complement(1037..1113)
                     /locus_tag="OO572_003976"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:tct)
     gene            complement(1215..1290)
                     /locus_tag="OO572_003977"
     tRNA            complement(1215..1290)
                     /locus_tag="OO572_003977"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Ile,seq:cat)
     gene            complement(1435..2256)
                     /locus_tag="OO572_003978"
     CDS             complement(1435..2256)
                     /locus_tag="OO572_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6662769.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2271..2627)
                     /locus_tag="OO572_003979"
     CDS             complement(2271..2627)
                     /locus_tag="OO572_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6662770.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(2640..>2990)
                     /locus_tag="OO572_003980"
     CDS             complement(2640..>2990)
                     /locus_tag="OO572_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6662771.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          809 a          831 c          597 g          753 t
ORIGIN      
        1 tctggaattt aatcaaaaac agccaggaat gccctacaaa tacagataac gtattgaatg
       61 aatgctttaa taaccgttgc actctgcaaa tatgccctta tggactaaaa caacaaagtc
      121 cataaggagt ttactcacat ctgacaaaat caatataaac agcccctccg gagaggggct
      181 ggagagtggc gctatgtgcc attccatggt gccgggtgcc tcccggtgaa ttcagtacca
      241 gcacctgaat ccgcgattat cccatatacc tactcgctga ttgcccctcc gcacaggggg
      301 attcaccatg ccagtttctt ttaacaaact ccccgcaaac cagacaacag tcaaccgcct
      361 gaattgtgaa gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt
      421 tttctttttc agcaacggga aagcaacaac caccacaccc gccaccagca caccgtcagc
      481 cggcactgac attatccggc tgctgcaatg ccattaacaa aaacagtaag caatcacttt
      541 ttaccgtaac aggtgataat ccagatatgt atctacccca gatgagtaat ccgaagttca
      601 tccataccac aggtcctggc tattctgttg tactcctgaa caagagcaaa taattctgaa
      661 ttagcaacca tgaactcatc gcaaaccctc tgtatagcat cactattcag aataataacg
      721 tctcttcccg aaagacgatc aggagtacag aacaaaactg tcaaacggct gaaggccttt
      781 gctcgtgctg cattgactat atcaatacgc tgcctaagga tgaaacaccc cgacgcctca
      841 tcaatattca ctctacccac accatatgaa tgataaatac ttaatgctga aaaaaccatt
      901 agaccgtata acaaatactc aatcaatact taacagaact tttatttttg acaaatatat
      961 aatattttca acaatatcct gagccaggta tatttcagta taaggctctg ccggaaggaa
     1021 tctggaagaa tgaatatggc gcgctgtact ggattcgaac cagtgaccga ttgcttagaa
     1081 ggcaattgct ctgtccggct gagctaacaa cgctgaatac cgataatgga ccgccatcgg
     1141 ggacccgccc cccgcaccaa caaccctgtt atcgtgtcgt ctgctcttcc tgataagcta
     1201 atggcggttt gtgatggtgg cccttgctgg atttgaacca gcgacctggc gattatgagt
     1261 cgctcgctct caccactgag ctaaagggcc gggagcagaa taataacggt ccgtaattaa
     1321 tttcgcaata aaaaaacccg ctcagcggcg ggttgtagaa actcttctaa cgccaggcat
     1381 aaaacgccca tcattatgac gaatttacca cagattccgg aaaaatcaac cttgttacct
     1441 agttaccttt tttaactgcc gctcagccca tgcttcttca atatcaaacc gggtcaccag
     1501 cgcatcataa aatttcttaa ctgttttttc ccatgacgcg cgtgttatct ggtttgtcac
     1561 ctcgcatata gcattaaatg cctccgttga tggtagtctt tcatagccac gaccaccaca
     1621 acgctggcag tctctgataa caggcatacc acgttttacc gactcttcac gatgaatggc
     1681 gacaccacgc ccacggcaat ccttacaggc ggtggaaacc tcaccctttc cgccacactc
     1741 cggacaggca acttttacca cctccctgac ttttttccat tcttcccagt aaaacggata
     1801 cacacctttc gtacactttg cccataccgg cggcttacca tccggatact ggatcttgtt
     1861 tgtaaaaact atgctttcaa taaatttttc cccatagcaa caagggcact gctttttact
     1921 cgctgcgctg cgggcataat cctcaaaagc gtacgaagcc ataatgcgca tcactaccgg
     1981 ttttatttct gccggaagtt ttctcaacgc cgccacacga tcgcaccgac tgagtgcata
     2041 atctgccagt aattctgttg cccgcgccct gtcattcata ctgatgccca ttttccccag
     2101 gaacgcagaa aaacccatct cagcccgatt ctgtgtcatg ccctgcgcgg ccatcacatc
     2161 agtgatactc agcgcatctt ttgacgttga ggccgatgca tcggtcaggc caggggattt
     2221 tggggagtag tatttcggta aatcttccag tttcattttt tgacctgctc ttaatgcatt
     2281 atggggtaaa tcttcacccc cagacgtcca ccagatactg gctgaccacg aacgatattg
     2341 atttcatcaa actgctcatc gtccattaac actcccgcat gcgtcagcgc atccagcggt
     2401 gctttcagga tattgtccag gtcgcgacga cgcttatccg gtggctctgc aatcaccttt
     2461 atcgccagcc ttccggacag gcttaatttc agccgctgct ggcgaacaat aagcgccaca
     2521 gcccggcgat aacgctttcc ctcctccgag ataaaatatg tgctgccacg gcgtcgccag
     2581 taagtgttca ccgtcggcgg gtaaggtaaa accaaatcta tgagcatcag tcacctcttt
     2641 tacccaagca cgccagttgc aaaggcgtga tcaagaaaac gaaaaattaa atcaacctga
     2701 gaaccatgct tttcttcgaa cgccagagga tccgcatgaa gctcgttgtg atgctcccga
     2761 cacagcggta gcgtgaaaat atcgtgagat ttggtcccca ttccgccctg accatgacca
     2821 atcaggtgat ggggatcgtc ggctggctta ccacaacacg cacacggctg tgtcttcacc
     2881 cagcgtttgt atttctcgtt aacccagcgg cgacgtttag gtcgtttcat gaaagattcc
     2941 ggagactcag gatcaacggc aatgctgacc accgtctttt cctgttgtgg
//
LOCUS       DAJTYL010000234         2981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYL010000234 DAJTYL010000000
VERSION     DAJTYL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.239"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..536
                     /locus_tag="OO572_003981"
     CDS             <1..536
                     /locus_tag="OO572_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6662772.1"
                     /translation="CHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTE
                     KLVAAIKSGKYDTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQL
                     LITADHGNAEQMRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLM
                     GMEIPQEMTGKPLFIVE"
     gene            546..1829
                     /gene="envC"
                     /locus_tag="OO572_003982"
     CDS             546..1829
                     /gene="envC"
                     /locus_tag="OO572_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6662773.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            1833..2792
                     /locus_tag="OO572_003983"
     CDS             1833..2792
                     /locus_tag="OO572_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6662774.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(2779..2981)
                     /locus_tag="OO572_003984"
                     /pseudo
     CDS             complement(2779..2981)
                     /locus_tag="OO572_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          764 a          790 c          832 g          595 t
ORIGIN      
        1 tatgccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc
       61 gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa atgagctccg
      121 cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac accattatct
      181 gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg gcggttaaag
      241 cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag tccgtgggtg
      301 gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat ccggcaacgg
      361 gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt ggtgataaga
      421 acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga
      481 tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg gaataatccc
      541 tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg cgcaggtttg
      601 caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt
      661 ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc gccgcaaaag
      721 agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc
      781 aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac acgctcaatc
      841 aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag cagcaaaaag
      901 ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag ggcgagcata
      961 ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt ttacaggctt
     1021 atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg
     1081 aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa caaacgctgt
     1141 tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag cgtaaaaaga
     1201 cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt gaactgcgtg
     1261 ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg aaagcgcgtg
     1321 ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa gcgacgcgca
     1381 aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga actggtggcc
     1441 tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg catcgctatg
     1501 gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc tctgaaggta
     1561 ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg caaggttacg
     1621 gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc tataatcaga
     1681 gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca ctggtgggca
     1741 gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag ggtcaggcgg
     1801 tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt aacgttcttg
     1861 catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt gccatcgtca
     1921 ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta
     1981 tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc aaagcgcata
     2041 acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa cagccgctgg
     2101 agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt attcgtagtg
     2161 cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc aagatgacct
     2221 ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat ctttacttcc
     2281 tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt actggcgtga
     2341 aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac atccgtgtgc
     2401 aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc attgggcatc
     2461 ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg ccagacatta
     2521 cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct acaccgccga
     2581 aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga
     2641 agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc gaaagcatca
     2701 gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc aaacagcccg
     2761 aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga
     2821 ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct ggtaaccgac
     2881 cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta aatatctcgg
     2941 caatcatccg ttggcgggta agaatatccg gctctttgcg c
//
LOCUS       DAJTYL010000235         2772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYL010000235 DAJTYL010000000
VERSION     DAJTYL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.240"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..789)
                     /gene="virF"
                     /locus_tag="OO572_003985"
     CDS             complement(1..789)
                     /gene="virF"
                     /locus_tag="OO572_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6662775.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            1218..1619
                     /locus_tag="OO572_003986"
                     /pseudo
     CDS             1218..1619
                     /locus_tag="OO572_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1801..2067
                     /gene="ospE1"
                     /locus_tag="OO572_003987"
     CDS             1801..2067
                     /gene="ospE1"
                     /locus_tag="OO572_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6662776.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            2457..>2772
                     /locus_tag="OO572_003988"
     CDS             2457..>2772
                     /locus_tag="OO572_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6662777.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTH"
BASE COUNT          938 a          516 c          480 g          838 t
ORIGIN      
        1 ttaaaatttt ttatgatata agtaaaattt ctttggagtt ataccataat attcattaaa
       61 tttccttata aaataagatg ggcttgatat tccgataagt cttgatacat cattaatata
      121 gctttgacta ttcaataaaa gctttgctgc atgatgcatg cgaatatcaa gaaggatttg
      181 ttgaaatgtt aatttttcac tctccaatcg ttttctaaca gctatttctg ataaattcaa
      241 gttatttgaa atatcagaaa gacgccatct cttctcgatg tttttttcaa caatcttcct
      301 tatctgatca gaaaaactaa gagaagaagc tatcgatatc gaagtatata aagcttcctc
      361 atcagaaaca gctgataaaa ggcaagctaa actatagatc tttcttttgc cgaaaggcat
      421 ctcttttata gatttgaaca aatcgataga aacctcctcc tcagagagga ggaatatttt
      481 tttgtttaac ccccttttct cctcagaata ggagtgttga aatgaataaa ttggttccat
      541 tattctaata atgcttaata ataaatttct gtcaaggctt ataatctcaa atggattaat
      601 gctatcagat ttttttatgg agacgtttat ttgtatattt cgctctataa aagcaatttg
      661 cccttcatcg atagtcaaag tttcattgcc tgagctaacc gtcattgaac acctttttgc
      721 atataaaata atatagttat gcaagcgaac ctttatatct attttgtttt tatgtcccat
      781 atccatcata aattcaacag aatataccaa actatattta gtgtttacag agtaactaag
      841 aaatttctgt aacagtgatt gtatacaaaa tctttccgtt attaagatag attaagtaat
      901 ttttcctata tttaacttta tttacatttt ttgaagaaag agcttatgca gcttctattt
      961 ctctgtgcaa caagctaagt atttgcactg aaagggacta taaccatatt atggtttcac
     1021 ttttcttagg caatatctgt gtaaatgttc ggaaaggagt ggagcttcgc aaaactccag
     1081 catcataata tcctatacgg aacgcataaa gccccttcag actggaggct ccacaaatcc
     1141 ggttggtgtt gatattggta agcgttaacc gaatgccgca ttgacgattc tcccgatgac
     1201 agcgttacgt tccatattgc gatcgtgaag ccatagactg ggcagcaggc acgggagatt
     1261 atgacaaagc aacggtgcag gatgtcatgt caggagcgat atggcgataa aggaccggaa
     1321 cggccaatcc agtggccgat aacggttcgg cttatacggc tcatgaaaca cggcagttcg
     1381 ccagagagtt gaatctggag ccctgcacga cggcagtcag tagccctcag agtaatggaa
     1441 tagcagaacg gttcatgaaa acgatgaagg aagactgcat cgcgttcatg ccgaaaccaa
     1501 gaacggcatt gcataacctt gcagtggcga tcgagcatta caatgaaaac catccgcata
     1561 gtgcgttggg ttatctctca ccaagggaat atcgacgtca gcgggtaatg tcaacttaag
     1621 atacaacgac tgtctgaata tcgtgggtca aggacagttc aatgacgtaa cgcagccatt
     1681 tttctggctc cacatctcat cgcttacgaa aaaagtatcg tttttttaca gtaaacttca
     1741 tttagccgac tataatgtaa aaaataaatt ctttactaaa atataacata aggaagcaat
     1801 atgcttacac aaactatatt cccttgcctt ccacaaaaac aagaaaatat catccttgaa
     1861 gtatcaaatc ctgtattatt atcttctact gttactacag atggatatac tgttttcaat
     1921 aaaaaagcag ctatatacga attacaaata cctgctgcca gccggacgaa aacgttgaaa
     1981 ttcactgcta cagaaatgca atggttgaca aagataaatg aagccggaat tgatgaaaaa
     2041 caaagccaac ggtattctga tttctgaagt taatttcaga ctcgtttaag ctccattttc
     2101 aaaaaaaata tatcagggtt tgttactaat aacttctgaa aacagaaaaa aatagagcag
     2161 taattctatt tcagagagga gtaactccat ctatcctcga tacgtactga acgttatcgt
     2221 gatatattca tcatgctttc ttcaccatta ttcgagtata gggagagacg caatgacctt
     2281 aaataccttt ttatggctga attaccattc tcaggaataa cagcagactc cttcccatag
     2341 acggcttagg gggaaagcaa tacccggaga aagggtacaa agcaaactgt tttaaaatta
     2401 ataagccata ttctatttaa ctagaattct tatcgttgat aagaattcta gttataatga
     2461 ttaaatcaac caatatacag gcaatcggtt ctggcattat gcatcaaata aacaatgtat
     2521 actcgttaac tccattatct ttacctatgg aactgactcc atcttgtaat gaattttatt
     2581 taaaaacctg gagcgaatgg gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct
     2641 tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta tctgagttag
     2701 atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata agaaaaaacc
     2761 tattaacaca tc
//
LOCUS       DAJTYL010000236         2741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYL010000236 DAJTYL010000000
VERSION     DAJTYL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.241"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..572
                     /locus_tag="OO572_003989"
                     /pseudo
     CDS             1..572
                     /locus_tag="OO572_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            565..2642
                     /gene="flhA"
                     /locus_tag="OO572_003990"
                     /pseudo
     CDS             565..2642
                     /gene="flhA"
                     /locus_tag="OO572_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2642..>2741
                     /gene="flhe"
                     /locus_tag="OO572_003991"
     CDS             2642..>2741
                     /gene="flhe"
                     /locus_tag="OO572_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6662778.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNH"
BASE COUNT          587 a          673 c          829 g          652 t
ORIGIN      
        1 gcactgctgg tggtgcttgg tgtcattcca atggtgggat ttgacgtctt tttccaaatc
       61 ttcagccacc tgaaaaagct gcgaatgtcg cggcaggata ttcgtgatga gttcaaacaa
      121 agcgaaggcg acccccatgt taaaggacgg atctgtcaga tgcagcgagc tgccgcacgg
      181 cgtcggatga tggccgatgt gccgaaagcg gatgtcattg tcaataaccc gacccactat
      241 tcggtagcgt tgcagtatga cgaaaacaaa atgagcgcac cgaaagtggt cgctaaaggt
      301 gcagggctgg tcgcgctgcg cattcgtgaa attggcgctg aaaataacgt cccgacgctt
      361 gaagcgccgc cgctggcgcg agcgctgtat cgacatgcgg agattggtca acaaatcccg
      421 ggtcaactgt acgccgcggt ggcggaagtg ctggcctggg tctggcaact gaaacgctgg
      481 cgtctggctg gtggacagcg ccctgtacac ctactcatct tccggtgccg gcagccctgg
      541 attttattaa cgagaaaccg acccatgagt aatctggccg cgatgctgcg cctgcccgca
      601 aacctgaaat cgacataatg gcagatcctt gccggaccga ttttgatcct gttgatcttg
      661 tcgatgatgg tgctgccact gcccgcattc atactcgacc tgttgtttac cttcaatatt
      721 gccttgtcga tcatggtgtt gctggtggcg atgtttaccc agcgcacgct tgagtttgct
      781 gcgtttccga ccattctgtt gtttactatg ttgttgcgtc tggcacttaa cgtggcttca
      841 acccgtatca ttttgatgga agggcatacc ggcgcggcgg cggctgggaa ggtggtcgaa
      901 gcgttcggtc acttcctcgt tggtggcaat ttcgctatcg gtatcgtggt gtttgtcatt
      961 ctcgtgatca tcaactttat ggtcattacc aaaggtgccg ggcgtatagc agaagtgggt
     1021 gcgcgctttg ttctcgatgg tatgccgggt aagcagatgg cgattgacgc cgaccttaac
     1081 gctggattga ttggtgaaga tgaggcgaaa aaacgccgct ccgaagtgac tcaggaagcc
     1141 gatttttacg gctcaatgga cggggcaagt aagtttgttc gcggcgatgc cattgccggg
     1201 atcctcatca tggtcattaa cgttgtcggc gggttgctgg tcggcgtgct gcaactggca
     1261 tgagcatggg acacgcggcg gaaagttata cgctattgac cattggcgac ggtctggtgg
     1321 cacaaattcc ggcgctggtg atttctaccg ccgcgggggg catcgttacg cgtgtcagca
     1381 ccgatcagga tgttggcgag cagatggtga atcagctttt cagtaaccca agcgttatgt
     1441 tgttaagcgc cgccgtgctc ggtttactcg gcctggtgcc tggaatgccg aacctggtat
     1501 ttttgctgtt cactgccgga ttgctggggc tggcctggtg gatacgcgga cgcgaacaaa
     1561 aagcgcctgc cgaacccaaa ccggtaaaaa tggcagagaa taataccgtt gtcgaagcga
     1621 cgtggaacga tgtacaactg gaagattctc tgggaatgga agtgggttat cgactgatcc
     1681 cgatggtcga tttccagcag gatggtgagt tgttgggccg tatacgcagt atccgcaaga
     1741 aatttgccca ggagatggga tttctgccgc cagtggtgca cattcgcgac aatatggatc
     1801 tgcaacctgc ccgctatcgc attttgatga aaggcgtgga gattggcagt ggtgatgctt
     1861 atccggggcg ctggctggcg attaaccctg gaaccgctgc cgggacgtta cctggtgagg
     1921 cgaccgtcga tccggcattt ggcctgaatg ctatctggat tgaaagtgcg ctaaaagaac
     1981 aggcgcagat tcaggggtac acagtggttg aggccagcac ggtggtagca acgcatctta
     2041 accacctcat tagccagcat gccgcagagc tgtttggtcg tcaggaggcg caacagctgt
     2101 tggatcgcgt cgcccaggag atgccaaagc tgacggaaga tctcgttcct ggcgtcgtca
     2161 cgctcaccac actgcataaa gtgctgcaaa atctcctcga tgaaaaagta ccgattcgcg
     2221 atatgcgcac cattctcgaa acgctggcgg aacatgcgcc catccaaagc gatccacatg
     2281 aattaagcgc cgtcgtgcgc gtggcgttgg gacgggcgat tacccagcag tggtttcctg
     2341 gcaaagatga agtccatgtt attggcctcg atacaccgct ggaacgtttg ttactacagg
     2401 cgctgcaggg cgggggagga ctggagccag ggctggcgga tcgtttactg gcgcaaactc
     2461 aggaagctct atcccgtcag gagatgctgg gtgcgccgcc agtattgttg gtgaaccacg
     2521 cgctgcgacc attattgtct cgcttcctgc gccgcagctt gccgcagtta gtggtcctgt
     2581 cgaatctgga actgtctgat aaccgacata tccgcatgac ggcgacaatt ggcggtaaat
     2641 gatgagaacc ttattagcga tattattgtt tccgctgctg gtgcaagccg ccggggaggg
     2701 gatgtggcag gcaagtagtg tcggaattac gctaaatcat c
//
LOCUS       DAJTYL010000237         2697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYL010000237 DAJTYL010000000
VERSION     DAJTYL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.242"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="OO572_003992"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="OO572_003992"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6662779.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2117)
                     /locus_tag="OO572_003993"
     CDS             complement(1725..2117)
                     /locus_tag="OO572_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6662780.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2478)
                     /locus_tag="OO572_003994"
     CDS             complement(2119..2478)
                     /locus_tag="OO572_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6662781.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          666 a          649 c          674 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctggtccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DAJTYL010000238         2682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYL010000238 DAJTYL010000000
VERSION     DAJTYL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.243"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            142..1002
                     /gene="tesB"
                     /locus_tag="OO572_003995"
     CDS             142..1002
                     /gene="tesB"
                     /locus_tag="OO572_003995"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6662782.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1051..2337)
                     /gene="amtB"
                     /locus_tag="OO572_003996"
     CDS             complement(1051..2337)
                     /gene="amtB"
                     /locus_tag="OO572_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6662783.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2367..>2682)
                     /gene="glnK"
                     /locus_tag="OO572_003997"
     CDS             complement(2367..>2682)
                     /gene="glnK"
                     /locus_tag="OO572_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6662784.1"
                     /translation="KPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAELYRGAEYS
                     VNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRIRTGEADEA
                     AL"
BASE COUNT          662 a          794 c          636 g          590 t
ORIGIN      
        1 aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt cagactattt
       61 cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct tgctgcggca
      121 gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac attgttaaat
      181 ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg tttacgccag
      241 gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac cgtcccggaa
      301 gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag taagaaaccg
      361 attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg ccgggttgct
      421 gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc accagaagcg
      481 ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct gccttcggaa
      541 acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga taaattcatc
      601 tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa aggtcacgtc
      661 gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga tgacctgcgc
      721 gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt agctctacag
      781 ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga ccattccatg
      841 tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga gagcacctcg
      901 gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg cgtgctggtt
      961 gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag ccggaggtga
     1021 aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct cgccgtggct
     1081 gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa ccgtcagatc
     1141 cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa tggcgatgct
     1201 ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc ccacgccgcc
     1261 cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac aaacgccgtg
     1321 cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca tggtaacgcc
     1381 ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa ccccaatgta
     1441 gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg ctcccagcag
     1501 agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg ccgccgccgt
     1561 tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc ccactgaccc
     1621 ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga ccatcggcag
     1681 gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca gatacgcgcc
     1741 caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga aatccagcgc
     1801 accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa tgtaagagag
     1861 cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt ccgccagcgc
     1921 cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta cgtggatata
     1981 ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca accagttaat
     2041 gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa ccacccagag
     2101 aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca cgtttttgcc
     2161 gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca gcaccagcgc
     2221 agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg cagccattac
     2281 cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta tcttcatttt
     2341 tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc gccggtacga
     2401 atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat ttttccggtg
     2461 taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc aatcgccaca
     2521 tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta cagctcggca
     2581 tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg aataccaatg
     2641 gaagataacg cttcacgaac gtcttccagc ttgaatggtt tg
//
LOCUS       DAJTYL010000239         2655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYL010000239 DAJTYL010000000
VERSION     DAJTYL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.244"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            9..2636
                     /gene="gyrA"
                     /locus_tag="OO572_003998"
     CDS             9..2636
                     /gene="gyrA"
                     /locus_tag="OO572_003998"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6662785.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
BASE COUNT          654 a          664 c          760 g          577 t
ORIGIN      
        1 gcggttagat gagcgacctt gcgagagaaa ttacaccggt caacattgag gaagagctaa
       61 agagctccta tctggattat gcgatgtcgg tcattgttgg ccgtgcgctg ccagatgtcc
      121 gagatggcct gaagccggta caccgtcgcg tactttacgc catgaacgta ctaggcaatg
      181 actggaacaa agcctataaa aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc
      241 atccccatgg tgacttggcg gtttatgaca cgatcgtccg tatggcgcag ccattctcgc
      301 tgcgttacat gctggtagac ggtcagggta acttcggttc catcgacggc gactctgcgg
      361 cggcaatgcg ttatacggaa atccgtctgg cgaaaattgc ccatgaactg atggccgatc
      421 tcgaaaaaga gacggtcgat ttcgttgata actatgacgg cacggaaaaa attcccgacg
      481 tcatgccaac caaaattcct aacctgctgg tgaacggttc ttccggtatc gccgtaggta
      541 tggcaaccaa catcccgccg cacaacctga cggaagtcat caacggttgt ctggcgtata
      601 tcgatgatga agacatcagc attgaagggc tgatggaaca catcccgggg ccggacttcc
      661 cgacggcggc aatcattaac ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg
      721 gcaaggtata tatccgcgcc cgtgctgaag tggaagttga cgccaaaacc ggacgtgaaa
      781 ccattatcgt ccacgaaatt ccgtatcagg taaacaaagc gcgcctgatc gagaagattg
      841 cggaactggt aaaagaaaaa cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca
      901 aagacggtat gcgcatcgtg attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca
      961 acaacctcta ctcccagacc cagttgcagg tttctttcgg tatcaacatg gtggcattgc
     1021 accatggtca gccgaagatc atgaacctga aagacatcat cgcggcgttt gttcgtcacc
     1081 gccgtgaagt ggtgacccgt cgtactattt tcgaactgcg taaagctcgc gatcgtgctc
     1141 atatccttga agcattagcc gtggcgctgg cgaacattga cccgatcatc gaactgatcc
     1201 gtcatgcgcc gacgcctgca gaagcgaaaa ctgcgctggt tgctaatccg tggcagctgg
     1261 gcaacgttgc cgccatgttg gaacgtgctg gcgacgatgc tgcgcgtccg gaatggctgg
     1321 agccagagtt cggcgtgcgt gatggtctgt actacctgac cgaacagcaa gctcaggcga
     1381 ttctggatct gcgtttgcag aaactgaccg gccttgagca cgaaaaactg ctcgacgaat
     1441 acaaagagct gctggatcag atcgcggaac tgttgcgtat tcttggtagc gccgatcgtc
     1501 tgatggaagt gatccgcgaa gagctggagc tggttcgtga acagttcggt gacaaacgtc
     1561 gtactgaaat caccgccaac agcgcagaca tcaacctgga agatctgatc acccaggaag
     1621 atgtggtcgt gacgctctct caccagggct acgttaaata tcagccgctt tctgaatacg
     1681 aagcgcagcg tcgtggcggg aaaggtaaat ctgccgcacg tattaaagaa gaagacttta
     1741 tcgaccgact gctggtggcg aacacgcacg accatattct gtgcttctcc agccgtggtc
     1801 gcgtctattc gatgaaagtt taccagttgc cggaagccac tcgtggcgcg cgcggtcgtc
     1861 cgatcgtcaa cctgctaccg ctggaacagg acgaacgtat caccgcgatc ctgccggtga
     1921 ccgagtttga agaaggcgtg aaagtcttca tggcgaccgc taacggtact gtgaagaaaa
     1981 ccgtcctcac cgaattcaac cgtctgcgta ccgccggtaa agtggcgatc aaactggttg
     2041 acggcgatga gctgatcggc gttgatctga ccagtggcga agatgaagta atgctgttct
     2101 ccgccgaagg taaagtggtg cgctttaaag agtcttctgt ccgtgcgatg ggctgcaata
     2161 ccactggtgt gcgcggtatt cgcttaggtg aaggcgataa agtcgtctct ctgatcgtgc
     2221 ctcgtggcga tggcgcaatc ctcaccgcaa cgcaaaacgg ttacggtaaa cgtactgcag
     2281 tggcggaata cccaaccaag tcgcgtgcga cgaaaggggt tatctccatc aaggttaccg
     2341 aacgtaacgg tttagtggtt ggcgcggtgc aggtagatga ctgcgaccag atcatgatga
     2401 tcaccgatgc cggtacgctg gtacgtactc gcgtttcaga aatcagcatc gtgggccgta
     2461 acacccaggg cgtgatcctc atccgtactg cggaagatga aaacgtagtg ggtctgcagc
     2521 gtgttgctga accggttgac gaggaagatc tggataccat cgacggcagt gccgcggaag
     2581 gggatgatga aattgctccg gaagtggacg ttgacgacga gccagaagaa gaataatttt
     2641 acttcttcat gccaa
//
LOCUS       DAJTYL010000240         2584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYL010000240 DAJTYL010000000
VERSION     DAJTYL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.245"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..717
                     /gene="lsrF"
                     /locus_tag="OO572_003999"
     CDS             <1..717
                     /gene="lsrF"
                     /locus_tag="OO572_003999"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6662786.1"
                     /translation="LAFDHGYFQGPTIGLERIDINIAPLFEHADVLMCTRGILRSVVP
                     PATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKN
                     IIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFER
                     IVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQA
                     VVHHNETADRAYELYLSEKQ"
     gene            741..1031
                     /gene="lsrG"
                     /locus_tag="OO572_004000"
     CDS             741..1031
                     /gene="lsrG"
                     /locus_tag="OO572_004000"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6662787.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1070..1828
                     /gene="tam"
                     /locus_tag="OO572_004001"
     CDS             1070..1828
                     /gene="tam"
                     /locus_tag="OO572_004001"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6662788.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1832..>2584)
                     /locus_tag="OO572_004002"
     CDS             complement(1832..>2584)
                     /locus_tag="OO572_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662789.1"
                     /translation="APFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLR
                     EVKTTLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPA
                     NRILLIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLIL
                     HRTVYLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLD
                     AICNAIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          661 a          612 c          694 g          617 t
ORIGIN      
        1 ctggcctttg accatggtta ttttcagggg ccgactatcg gacttgaacg cattgatata
       61 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
      121 agcgtagttc ccccggcgac caataagccg gtggtactgc gggcgtcagg tgcgaactct
      181 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
      241 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
      301 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
      361 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
      421 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
      481 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
      541 gagacgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
      601 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
      661 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
      721 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
      781 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
      841 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
      901 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
      961 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     1021 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     1081 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     1141 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     1201 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     1261 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     1321 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     1381 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     1441 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     1501 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     1561 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     1621 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     1681 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     1741 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     1801 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     1861 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     1921 tgcagatggc atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt
     1981 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     2041 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     2101 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     2161 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     2221 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     2281 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     2341 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     2401 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     2461 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     2521 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     2581 gagc
//
LOCUS       DAJTYL010000241         2558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYL010000241 DAJTYL010000000
VERSION     DAJTYL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.246"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            187..765
                     /locus_tag="OO572_004003"
     CDS             187..765
                     /locus_tag="OO572_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6662790.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            809..2284
                     /gene="ampG"
                     /locus_tag="OO572_004004"
     CDS             809..2284
                     /gene="ampG"
                     /locus_tag="OO572_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6662791.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT          534 a          661 c          638 g          725 t
ORIGIN      
        1 agcgtatgaa gtttactgat ttaaagattg aaacacaggc ctcgcatcag cgtttctttt
       61 accaggggtg attatttcgt caacatgatg aatttacatg ccttacccac ttcccctcgc
      121 cgttggcaat gttatgatgg cgggaatttt taccaaacac gcttaagagt ctctgagagc
      181 ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc tgtttatgct tgcaggatgc
      241 gcaaaaccgc caacaactat tgaagtttcc ccgacgatta cactgccaca gcaggatcca
      301 agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc agcgtaccga tcaggcgctg
      361 gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg cctcccgcgc tctgcgtttc
      421 ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg gttacatggt tggtccgaat
      481 ggcccggtta atctgcaaat catcgttagc caactgtatg ctgacgtgtc ccagggcaac
      541 gtgcgctaca acatcgctac caaagcggat atcgccatca tcgctaccgc gcagaatggc
      601 aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg aaggtgcgtt ccaggcctcc
      661 aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg ataccatcgc tgatatgtct
      721 caggacacca gcattcacga attcatcaag cagaacgcgc gttaatttct gccctctggc
      781 ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa tatttacgta tttttcaaca
      841 gccgcgttca gccatattgc tgatcctggg ttttgcttcc gggctaccgc tcgccctgac
      901 atccggcacc ttacaggcct ggatgacggt cgaaaatatc gatctcaaaa ccattggttt
      961 cttctctctg gtaggccagg cttacgtttt taaattcctc tggtcaccgc tgatggaccg
     1021 ctacacgccc ccattttttg ggcggcggcg cggttggctg ctcgccacgc aaatcctgtt
     1081 attagtcgcc attgcggcga tgggttttct cgaaccaggc acccaactcc gctggatggc
     1141 ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag gatattgtct tcgatgcgtg
     1201 gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc gcggcaatca gcgtgctggg
     1261 ttaccgttta gggatgctgg tttccggcgg cctagccctg tggctggcag ataaatggtt
     1321 gggctggcag ggcatgtact ggttgatggc ggcgctgttg atcccctgta ttatcgcgac
     1381 gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc aaaacgctgg aacaagcggt
     1441 tgttgcacct ctgcgagatt tctttggtcg caataatgcc tggcttattt tgcttcttat
     1501 cgtgctgtat aagctgggcg acgcattcgc catgagcctg acaaccacgt ttttgattcg
     1561 cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac aaaacgcttg gcttactggc
     1621 gaccattgtt ggcgcattgt acggtgggat tttgatgcag cgcctgtcgc tgttccgggc
     1681 gctgctgatt ttcggcattt tacaaggtgc gtctaacgct ggttactggc tgctgtcgat
     1741 tactgataag catctctaca gcatgggcgc agccgtcttt ttcgaaaacc tctgtggcgg
     1801 gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg tgtaataagt cattttccgc
     1861 tactcaattt gccctgctct cagcgctttc cgctgtaggg cgagtttatg tcggccccgt
     1921 ggcgggttgg tttgttgaag cacacggctg gtcgacattc tatctattct ccgtcgccgc
     1981 tgccgtaccg gggcttattt tgctgctggt ttgccgccag acgcttgaat atacacgagt
     2041 aaatgacaac tttatctccc gtaccgaata tccggcaggt tatgcctttg ccatgtggac
     2101 actggcggcg ggcgtcagcc tgttggccgt gtggttactt ctgttgacga tggacgcgct
     2161 ggatttgacg cacttctctt tcctgcctgc tctgctggaa gtcggggttt tagtcgccct
     2221 ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg ctacgaaaaa cgcatctgac
     2281 gtaatctgta aatattattt agctgtagta atcactcgcc gtaattatta cggctaaata
     2341 aacatcagca tcgcttatta atatttgttc ttttgtgcgg cttagcgttt ggaaattatt
     2401 gacgccattt ataaattcta tttttcttac acgattcagc taatgagtct ttattttctc
     2461 accacccagt tgtcacttta atgataatta tttgttaaat aattgtttta tttcgcattg
     2521 gttataccaa ttgcccacca agactccttc tgcactcc
//
LOCUS       DAJTYL010000242         2544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYL010000242 DAJTYL010000000
VERSION     DAJTYL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.247"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1269)
                     /locus_tag="OO572_004005"
     CDS             complement(<1..1269)
                     /locus_tag="OO572_004005"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6662792.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSI"
     gene            complement(1353..2516)
                     /locus_tag="OO572_004006"
     CDS             complement(1353..2516)
                     /locus_tag="OO572_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6662793.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
BASE COUNT          630 a          763 c          610 g          541 t
ORIGIN      
        1 aatactgttt accggcatct cccggtaaag ccgcggcagc tcgctctcct cgcaataacc
       61 aatgccgccc agcacctcca tcgcttcggc cacaaacggc atacctcgtt tgcagatcac
      121 aaattttgcc gcaggcgtaa ataaacgcgc ccacagggct tctttggcat cggcacgccg
      181 gtcccacgct cgcgcaagac gaaacagcaa cgccgtttgc ccttcaagct gaagtgccat
      241 gcgacttaag acatgacgca taaggggctg ttggatcaat ggattaccaa aaacatggcg
      301 ttgatgtgca tgataaatcg ccagcgaaaa tgcacggcgc atcatggcat ggctacccag
      361 ggcgcaatca aaacgcgtca tcccccccat tttcaggatc agacgaattc cttccccttc
      421 ctgccccaat aaccagccaa tggcatcctg aaactccact tcgcaactgg cgttagagcg
      481 attacccagc ttatctttca gccgctcgag gcgaatcgcg ttgcgttgcc cgtcaggcaa
      541 aaagcgcggc acaaaaaagc aggacagacc gcccgcggtc tgcgccagca ccagatgcgc
      601 atcgctttgc ggcaccgaga aaaaccattt atgccccacc agccgataag agccatcttc
      661 caaacgctct gcacgggtgg tgttgctcat gacatcggaa ccgccctgct tttccgtcat
      721 tcccatgcca atcaacaaac cgcgtttttg cccacctggc aataagtgag aatcgtagcg
      781 atcgctcagc agcggcgtgg tccagtcctg aaacggcgtg ggtaacatct gtaacaacaa
      841 tggcgtggcg gcaaaggtca tcgttactgg acataacgac ccagcctcaa cctgcgcatg
      901 taacataaaa cgcgccgcgc gcgccacaaa tgcgccggag cgagcgtctt cttcccaggc
      961 aagattgtgc acccgattgg tacataacgc ctgcatcagc aggtgccatg cgggatggaa
     1021 acgaacatcg tccagacgcc gcccctgggc atcgtagcgc aacagttcag gcgggtttac
     1081 attcgccagc cgccccagtt caagggattc agccgttcct aactgctgac caatactggc
     1141 tagaaaatcg ctatcccagc cagcaccttc acgcgttacc gcttcgcaaa gcgcgccatc
     1201 agacaggtat aagttgctgt tatttaatgg tataggttga ttaaaaatgg tgtgagtttg
     1261 ccagtgcact gtgtctccct ccatcaatgg cagtcaccat tagtatggtc actgccatgg
     1321 attcatgaca gaaagagatt gctaaaacat tcttatccag caatgtaatg cggaacgaaa
     1381 cgtgaggtgt ctttggtgat cagtgtgtta tcttcacgga tccccattcc gcacgcttca
     1441 tcatcgacaa tccagctacc gatgagtgtg tagctatcgc caaatcgcgg cagaggttga
     1501 aacgcctggt agatcatcgg ttcatcggcg taatcaccat cagcgtggtc aacgacgtta
     1561 ttctgaccgt cgaaaatggt gacgtttccg ccttcgcgcg agtagatcgg tttacgcaca
     1621 tagctttcgc cagcggcaat ctgcgatttt tcaccctcaa accaggatgc aagaaggtta
     1681 ggatgaccag ggaagaagcg ccaaagcagc ggcattagcc ctttattact caagatactt
     1741 ttccataacg gctccaccca ttgctcgcga cgcttgcgca gcagcggacc gttatcgtca
     1801 cgcatcatcc actccagcgg atacagctta aatgcacgct ggatgacatt atcatcaaga
     1861 tcggtcagta cgccgccgac gcccaaaccg agatcttcaa tgtagataaa ccgcgactcc
     1921 tgccctgcct gctgggcgca gtcctgcaag tacagcacgg tactcctgtc ttcatcggtg
     1981 tcctgacagc agcaaaaata aaacagttcc cgactgtaaa gctcactaaa gcgcgaaatc
     2041 aggcgttcct gaatggcgtt gtactgatcg gcatcacgcg gaataatgcc gctgcgccgg
     2101 gcatcctcca gccacaacca ctggaaataa gccgactcat acaacgaggt cggcgtatcg
     2161 gcgttgtact ccagcagctt caccggcgca ttgccacacc aggcaaaatc catccggcca
     2221 tacagcgaag gatcgcgtgc gcgccagctt tcggcgatca catcccagta caacggcggg
     2281 atcgccagtt gcgtcaggat ctcttcgtct ttaaccgcgc gatccaccac ctcaaggcac
     2341 atctgatgca gttccgcagt cggtttttcg atctgctctt caatctggcg cagagtaaaa
     2401 cggtaagccc gactctcgtc ccaatagatt tcattgtcga tgatatgaaa gtcgaaaccg
     2461 ttgttagcgg cgatctggtc cagatcccgc cgcacaggaa cgttgtgtct cagcatgatt
     2521 agcctcccca atgcccacgg gcgc
//
LOCUS       DAJTYL010000243         2434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYL010000243 DAJTYL010000000
VERSION     DAJTYL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.250"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(86..1510)
                     /gene="sbcB"
                     /locus_tag="OO572_004007"
     CDS             complement(86..1510)
                     /gene="sbcB"
                     /locus_tag="OO572_004007"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6662794.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            1692..>2434
                     /locus_tag="OO572_004008"
     CDS             1692..>2434
                     /locus_tag="OO572_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6662795.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLAT"
BASE COUNT          604 a          603 c          600 g          627 t
ORIGIN      
        1 gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg
       61 ccgtaccagc ggcggcggct tcaaattaga cgatctcttc cgcgtactgc caaagtgctt
      121 ttaacagcgc cactttctct ttgtcatcgg catattgttg taccagcatc tgcaattcat
      181 cagcataacc ctgcaaaaac tctggcgtga agacctggtg gcggtgctcc agccagcgtt
      241 gctgctcggc ataatccagc gtccccggga agttgcgtgc ccgataattg aacaacaact
      301 tttcaattcg tttatcaaca aaagtgatat ccagtgccgg taaattacgc ggctcggttt
      361 ccagcacaat tttcattgct gcacgatcgg cgtcactgaa aaagccatta taaagctgtg
      421 catccacatt atctgaaggt gtaaacggtt cggcttccgc gaatatcgct accacttttt
      481 cacgcacttg cggattttca cgcagaattt ttaggttatc gaggcaatgc tgacgattaa
      541 ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc ctgggccagt accggacatt
      601 tattgatatg caccagctta accggaacgg cggcgttatc gccaagatcg gcttttgcgg
      661 tatataaacg ctcgcgcaat gtgtcgatat ccagctccag taatggcgaa atgtctcctg
      721 ccaaatccgc cataattacg gcattgcgat tttcaggatg ccacgccagc ggtgccaccc
      781 agctggtatt gccacgccat gccccaaaca ttccggaaac gtgagccagg ggtttcatct
      841 gcggaacatc aatcaacgcc atcagtttgt gtttattacg atgggtaaag agataatcaa
      901 acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc aatagtggcg tacacatcag
      961 ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc tttggttaaa tgctcaaggc
     1021 gaaagctcgg tagaccgtca tcattttcag gccagtttat tccttccggg cgcagggcat
     1081 aacaggcacg cataacatcc agtaaatccc agcgcgagtt atcatgctgc cagctccagg
     1141 cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt gacttcgtcg tcgaaacgca
     1201 cattgttgta gcccagaata caggtcttcg gtacggtaaa aagcgagtga atacgggcgg
     1261 caaacgcggc ttcgttttct cctttcgccc gtgcctcctg cggggtaata ccggtaatta
     1321 atactgctcc aggccggggt aaatagtcat ccgcgggctt gcagtaaaag acttcgggtt
     1381 cgccgatgac attgaattcg tcatcggtgc gaatggctgc gaactgtgca gggcgatcta
     1441 acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg
     1501 tgtctgtcat tgtctagcat tgtccactgt agttaattaa ctgtctgtta taactcgaaa
     1561 agcaaccctt ttttcacctg agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg
     1621 ctgttgtcca tgtgatattg tgtaccagat gtgtgccaaa ctattatttc tgagcgtacc
     1681 aaatattatt tatggctata agcgacacaa aactgcgtac tatttatggt aaaccatatt
     1741 cgggcccaca agaagtggct gatgccgatg gcctcagcgt acgaatttca ccgaaggggg
     1801 tcatccagtt ccagtaccgc tatcgctggc atggcaagcc taatcgactt gggcttggtc
     1861 gatacccatc cctgtctttg aaggatgcca gacagatcac tgctgacttg cgaaagctct
     1921 atttctcagg aacggatcca cgcacctatt ttgaagagaa ggtggagaac tccatgacgg
     1981 tcgcccagtg tctcgactac tggttcgaca actacgtctc tacaactctc agagaaaaga
     2041 cccaggcact ttatcgatca acggttatga agcgcatgca tgacgccttc cctaatcgtc
     2101 cggcatcttc tatcacggtt aagcaatggg ttgacctgct taccgaagaa gaaaaagata
     2161 atccacgccg agcaaggcag gtgctaagtc aactaagatc agcaattagt tggtgcatgc
     2221 ggcgtcagtt gatagatagt tgcgcaatta tgagcatcca gccaagggac ttcggctccc
     2281 gcgctgaggt aggggatcgg gtactgtcgt atcacgaact ggctaagatt tggcttgcta
     2341 ttgaaagaag ccgtgcgtct acgtcaaata agctccttca tcagatgctt atgctgtggg
     2401 gggcgaggct ctcagagctt aggctggcaa caaa
//
LOCUS       DAJTYL010000244         2424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYL010000244 DAJTYL010000000
VERSION     DAJTYL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.251"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            97..1710
                     /gene="mppA"
                     /locus_tag="OO572_004009"
     CDS             97..1710
                     /gene="mppA"
                     /locus_tag="OO572_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6662796.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1761..>2424)
                     /gene="ynaI"
                     /locus_tag="OO572_004010"
     CDS             complement(1761..>2424)
                     /gene="ynaI"
                     /locus_tag="OO572_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6662797.1"
                     /translation="ITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNF
                     FSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSIS
                     VENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFAD
                     SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQ
                     GR"
BASE COUNT          687 a          553 c          589 g          595 t
ORIGIN      
        1 cttcctttgt cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat
       61 tacgcatcaa ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagttacg
      121 tgttgtgcgc tgttggtcag cagcatttct ctttcgtatg ctgcagaagt tccgagcggc
      181 acagtactgg cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg
      241 gatcccgcta aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaaggt
      301 ctggtgaatc agaacgaaaa aggggagatt gtccccggcg tcgcgactca atggaaaagt
      361 aatgacaacc gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca
      421 ccggtaacgg cgcaagattt tgtctacagc tggcaacgtc tggtggaccc aaaaacattg
      481 tcgccatttg catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat
      541 ggtaaagcta cgcctgatca gcttggcgtc accgcagttg atgcccatac tttgaaaatt
      601 cagcttgata aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg
      661 gtgcaaaaag ccaacgtaga aagcggtaaa gagtggacga aacccggaaa tctgatcggc
      721 aatggcgctt atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt ggtaccgaat
      781 acccattatt gggataacgc caaaacggtg ctgcaaaaag tgaccttcct gccaattaat
      841 caggaatccg cagccactaa gcgttacctc gcgggggata ttgatatcac cgaatccttc
      901 ccgaaaaata tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccaccg
      961 cagctcggga cctattatta tgcctttaac acgcaaaaag ggccgacggc agatcagcgc
     1021 gttcgcctgg cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttgggg
     1081 acgggcgaaa agccagcgtg gcattttata ccagatgtta ccgccggatt tacgccggaa
     1141 ccttcgccgt ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg
     1201 agcgcagctg gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca
     1261 gaaaaccatc aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta
     1321 gatgttaaat tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat
     1381 tttgatgtta tccgcgcctc atgggtgggg gattataatg aaccctccac tttcctgaca
     1441 ttattaacgt caatgcattc aggaaatatt tcacgcttta acaatccggc atacgacaaa
     1501 gttctggccc aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg
     1561 gcagaaaaaa tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga
     1621 cgattaatca aaccgtggct gaaaggttat cccattaata accctgaaga tgtagcgtac
     1681 agtcggacta tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga
     1741 taacgccagt gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt atccatatac
     1801 agcgtctggc tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc gataatcttc
     1861 aaataaacgt cttgctgtgc agcaagccac tcagcccata ccgtggtttt ggtaaagcaa
     1921 taaaccataa tattcaatga cgagtcagca aactggttga aataaaccag taaggtttgt
     1981 cgctggtcga tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc
     2041 acttttgccg catcctcata acgtaaacca atggtcgtgg taatgcggcg gttggtcatt
     2101 cgtcctgggt tttctacgct gatcgacgaa aacagcgagt tcggtacgta caatggacga
     2161 ttatcaaagg tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata
     2221 tttctgtccg gtgaacggat ccagtcgcca atactgaaag gacggtcgaa atagagcatg
     2281 atcccggaaa agaaattact cagaatatct ttaccggcca taccgacagc cagaccacca
     2341 ataccaccaa aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc ataaagcaga
     2401 acaagcacta caataatggt gatt
//
LOCUS       DAJTYL010000245         2412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYL010000245 DAJTYL010000000
VERSION     DAJTYL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.252"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..599
                     /locus_tag="OO572_004011"
                     /pseudo
     CDS             1..599
                     /locus_tag="OO572_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(641..2080)
                     /gene="aldA"
                     /locus_tag="OO572_004012"
     CDS             complement(641..2080)
                     /gene="aldA"
                     /locus_tag="OO572_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6662798.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2277..>2412)
                     /locus_tag="OO572_004013"
     CDS             complement(2277..>2412)
                     /locus_tag="OO572_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6662799.1"
                     /translation="SDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR
                     "
BASE COUNT          616 a          648 c          563 g          585 t
ORIGIN      
        1 gacgacactc tggatggcaa cgacaccatt gtttccgtgg cgtcatgcac cactaactgc
       61 cttgcgccga tggccaaagc cttgcatgac agtttcggga tagaagtcgg cacgatgacg
      121 accattcatg cctatactgg cacccagtca ctggtggatg gcccgcgtgg taaagattta
      181 cgtgcttcac gcgcagcggc agaaaatatc attccccaca ctacgggggc ggcaaaagcc
      241 attggtctgg tgatcccgga actgagcggc aaactgaaag gtcatgcgca acgcgtgccg
      301 gtgaaaacag gttcggtcac tgagctggtg tccattctcg gaaaaaagtg actgccgaag
      361 aggtgaataa cgcacttaaa caagcaacca ccaataacga gtcatttggt tataccgatg
      421 aagaaatagt ctcttccgat atcattggca gccatttcgg ttcagtgttt gatgccacgc
      481 aaacggaaat taccgccgtg ggcgatttac aactggtgaa aacggtcgcc tggtacgata
      541 acgaatatgg cttcgtcacg cagcttattc gcaccctcga aaaattcgct aaactctgac
      601 gcgcacaggc ggaggaaaaa acctccgcct ctttcactca ttaagactgt aaataaacca
      661 cctgggtctg cagatattca tgcaggccat gtttaccatc tgcgccgcca ataccggatt
      721 tacgccatcc ggcgtggaaa ccttgcatag cttcgaagtt ttcacggttg atgtaagttt
      781 caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg gtatagattg
      841 atgaggtcag gccgtaatca ctgtcattag ccattgagat agcatcttcc agcgtgtcaa
      901 atgcgacaac tggcagcacc gggccaaagg tttcctcatg cataatcgac atttcctggc
      961 gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc gctttgccac
     1021 ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctca accctttcca
     1081 gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg gggttaccaa
     1141 attgaaccgc ctgcaacgct tcacccagca gattgacgaa ctgatcataa atgcctttct
     1201 gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg cgtgaatcaa
     1261 cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc ggtgctttac
     1321 cacccaattc cagacacact ttggtgatgt ttttcgccgc agtcgccatg atcttctcac
     1381 ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc gccagttctt
     1441 gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga aggcctattt
     1501 catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg ggtttaatga
     1561 cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg aagaacggaa
     1621 agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaga agaatatttt
     1681 ctcccggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac tccgccatgt
     1741 aatcgatata gtcggcagta aaagccactt cgacttcagc tagctgctgg atcttgcccc
     1801 cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatcccg gcggagattt
     1861 tgcacaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt tgtgcacgtt
     1921 ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtatg cgggaaatga
     1981 cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag gtaacaaact
     2041 gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactct tgtgatttat
     2101 atgtttgttt tcggtgaata tattgttaat taacatcaat catcagagag acggcatcgg
     2161 gaaaggacaa gtctgtggag ggcttcataa aaacaagtgt aaaaggaata aaaggcagaa
     2221 ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg caatttttaa
     2281 cgtaacgagc gactttccat cgcctcgatg agcaccgcat catgtttcag cgtttgccat
     2341 gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc gcggggaccg
     2401 tagccatcgc ta
//
LOCUS       DAJTYL010000246         2332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYL010000246 DAJTYL010000000
VERSION     DAJTYL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.253"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..893
                     /locus_tag="OO572_004014"
     CDS             <1..893
                     /locus_tag="OO572_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6662800.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..>2332
                     /locus_tag="OO572_004015"
     CDS             894..>2332
                     /locus_tag="OO572_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6662801.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQV"
BASE COUNT          638 a          594 c          608 g          492 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg ca
//
LOCUS       DAJTYL010000247         2258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYL010000247 DAJTYL010000000
VERSION     DAJTYL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.254"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO572_004016"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO572_004016"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6662802.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          558 c          668 g          458 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcc
//
LOCUS       DAJTYL010000248         2240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYL010000248 DAJTYL010000000
VERSION     DAJTYL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.255"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(64..822)
                     /locus_tag="OO572_004017"
     CDS             complement(64..822)
                     /locus_tag="OO572_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6662803.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO572_004018"
     CDS             complement(830..1933)
                     /locus_tag="OO572_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6662804.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGRIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2240)
                     /locus_tag="OO572_004019"
     CDS             complement(1943..>2240)
                     /locus_tag="OO572_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6662805.1"
                     /translation="RSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVI
                     GYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          612 a          602 c          515 g          511 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcggccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga
//
LOCUS       DAJTYL010000249         2100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYL010000249 DAJTYL010000000
VERSION     DAJTYL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.256"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            46..1077
                     /gene="mdtN"
                     /locus_tag="OO572_004020"
     CDS             46..1077
                     /gene="mdtN"
                     /locus_tag="OO572_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6662806.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1077..>2100
                     /gene="mdtO"
                     /locus_tag="OO572_004021"
     CDS             1077..>2100
                     /gene="mdtO"
                     /locus_tag="OO572_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6662807.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADA"
BASE COUNT          453 a          578 c          574 g          495 t
ORIGIN      
        1 atgctgttct ggctggcatt tttctaatct atttgagacg cgctcatgga aagtacgccg
       61 aaaaaagcac ctcgcagtaa attccctgct ctgttagtgg ttgcgttggc gctggttgcc
      121 cttgttttcg ttatctggcg cgtagacagt gcgccatcaa ctaatgacgc ttacgcgtca
      181 gcagatacca ttgatgtggt gccggaagtc agcggtcgca ttgtagaact ggcggtcacc
      241 gacaaccagg cggtgaaaca gggcgatttg ctgttccgca tcgacccgcg cccgtacgaa
      301 gccaacctgg cgaaagccga agcctccctc gcggcgctgg ataagcaaat tatgctcacc
      361 cagcgtagcg ttgacgccca acagtttggt gccgactcgg ttaatgccac ggtagaaaaa
      421 gcccgtgccg ccgcgaaaca ggccacagat acattacgcc gcaccgagcc attactgaaa
      481 gagggttttg tctcagcaga agacgttgac cgtgcaagaa cggcgcagcg cgccgcagaa
      541 gcggatctta atgccgtatt gttacaggcg cagtcagccg ccagcgccgt cagcggcgtc
      601 gatgcgctgg tggcgcaacg tgcggcggtc gaagcggata ttgccctgac caaactacat
      661 ctggaaatga cgaccgttcg cgcgccattt gatggccgga tcatttccct caaaacctcc
      721 gtcgggcaat ttgcttctgc catgcgccct atttttaccc tcatcgacac tcgtcactgg
      781 tacgtgatcg ccaacttccg cgaaaccgat ctgaaaaata ttcgctcagg tacacccgca
      841 acgattcgcc tgatgagtga cagcggcaaa accttcgagg gtaaagtgga ttcgattggc
      901 tacggcgtgc taccggatga cggcggcctg gtgctgggcg gcctgccaaa agtgtctcgt
      961 tctattaact gggtccgcgt tgcccagcgt tttccggtca aaatcatggt cgataaacct
     1021 gacccggaaa tgttccgcat cggcgcttcg gcagtcgcta atcttgagcc gcaataatga
     1081 gcgcgctcaa ctccctgcca ttaccggtgg tcaggctgct ggcgttcttt catgaagagt
     1141 taagcgagcg gcgacccggc cgcgtgccgc agatcgtgca actctgggta ggctgcctgc
     1201 tggtgattct gatctcgatg acttttgaga tcccttttgt ggcgttatcg ctggcagtgc
     1261 tgttttacgg tattcagtcg aacgcgtttt acaccaaatt tgtggcgatc ttgtttgtgg
     1321 ttgccacggt gctggagatc ggcagcctgt ttttgatcta caaatggtca tacggcgaac
     1381 cgttgatccg attgatcatc gccgggccga tcctgatggg ctgcatgttt ttgatgcgca
     1441 cccatcggct gggactggtc tttttcgctg tcgccattta cgggcaaacc ttccccgcca
     1501 tgctcgacta tccggaagta gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct
     1561 atccaacctt gctgatgacg ttaatcggcg tgctgtggtt tcccaatcgt gccattacgc
     1621 aaatgcatca agcgcttaat gatcggcttg atgatgccat tagccacctg acggacagcc
     1681 tcgcaccgct acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa aaactgaatg
     1741 tcttttgtct cgcggacgat gccaactggc gaactcaaag cgcatggtgg caaagctgcg
     1801 tggcaacggt aacctacatt tactcgacgc tgaatcgcta cgatcccacc tcttttgctg
     1861 attctcaggc aattattgaa tttcggcaaa aattagcctc agaaatcaac aagctgcagc
     1921 atgccgttgc tgaaggtcag tgctggcaaa gcgactggcg gctcagtgaa agtgaagcgg
     1981 tggcggcacg ggaatgtaac ctggagaata tctgccagac gttgttacaa ctgggccaga
     2041 tgaacccgaa tacgccgcca acgcccgccg ccaaaccgcc atcaatggtc gccgatgctt
//
LOCUS       DAJTYL010000250         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYL010000250 DAJTYL010000000
VERSION     DAJTYL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.257"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..513
                     /gene="agaC"
                     /locus_tag="OO572_004022"
     CDS             <1..513
                     /gene="agaC"
                     /locus_tag="OO572_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6662808.1"
                     /translation="GLGLPFSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLN
                     WTTMLIVASAYAVIAFLCTYLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLR
                     VMFKAQYIPYLIAGFLFVCYIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASK
                     NEEEDYSNGI"
     gene            503..1294
                     /gene="agaD"
                     /locus_tag="OO572_004023"
     CDS             503..1294
                     /gene="agaD"
                     /locus_tag="OO572_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6662809.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1295..2050
                     /locus_tag="OO572_004024"
     CDS             1295..2050
                     /locus_tag="OO572_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6662810.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          498 a          504 c          537 g          555 t
ORIGIN      
        1 ggtcttggcc tgccgtttag tttgttaatg cagtacgtca ttctgttctt ctattccgct
       61 ttctcattat ttatgaccaa agccgataaa tgcgcgaaag aggcggatac ggcagcgttt
      121 tcccggctta actggacaac gatgctcatc gtcgcttcag cgtatgcggt gattgctttc
      181 ctctgtactt acctggcaca aggggcgatg caggcgctgg tgaaagcgat gcccgcctgg
      241 ttgacccacg gctttgaagt ggctggcggt attctgcctg ccgttggttt tggcttgctg
      301 ctgcgcgtaa tgttcaaagc gcaatatatc ccttacctga tcgccggttt cctgtttgtt
      361 tgctacatcc gggtcagcaa cctgttgccg gttgccgtac tgggcgcagg ctttgcggtg
      421 tatgagtttt tcaatgcgaa atcccggcag caagcgcaac cgcagcccgt tgccagtaaa
      481 aatgaagaag aggactacag caatgggatc tgaaatcagt aaaaaagata tcacccgtct
      541 gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac gaaaggatgc aggcgggcgg
      601 ttttacctgg gcgatgttgc cgatcctgaa aaagatttat aaggacgaca aaccgggctt
      661 aagcgcggcg atgaaagata acctcgaatt tattaatacc cacccgaatc tggtcggatt
      721 cctgatgggg ttattaattt cgatggaaga aaaaggagaa aaccgcgaca ccattaaagg
      781 cctcaaagtg gcactgtttg gcccaatcgc cgggattggc gatgcgattt tctggtttac
      841 tttgttgccg attatggcgg gaatttgctc atcatttgcc agccagggaa acctgctggg
      901 gccgattcta tttttcgccg tttacctgct tatctttttc ctgcgcgtcg gctggaccca
      961 cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg cgagagaact cgcagatgat
     1021 tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc gggctgatcg cttcgtatgt
     1081 gcatattaac gtggtgacat cgtttgccat cgacagtacc cacagcgtcg cgctgcaaca
     1141 ggatttcttc gataaagtct tcccgaacat tttaccgatg gcctacaccc tgctgatgta
     1201 ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta atcggcgtga cttttgtgct
     1261 ctctattgtt tgttccgcat tcggcatttt gtaaatggaa cgaggcactg cttctggcgg
     1321 tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt cagcaagttg aaaactatac
     1381 ggcgttaagt gaacgagcca gcgaatattt attggctgtg atccgtagca aaccggatgc
     1441 cgtgatttgc ctggcgaccg gagccacgcc attactgacg tatcattatc tggtagaaaa
     1501 aatccaccag cagcaggttg atatcagcca gctcaccttc gtgaagctcg acgaatgggt
     1561 ggatctgcca ttaacgatgc ctggcacctg cgaaactttc ctacaacagc atatcgtgca
     1621 gccgctgggg ctacgtgaag accagctcat cagctttcgc tccgaagaga taaatgagac
     1681 agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc ggtctggatt tatgcgttct
     1741 cggattgggg aaaaacggtc atcttgggct gaacgaaccg ggagaaagcc tacaaccggc
     1801 ctgccatatc agtcaacttg atgccagaac acagcaacat gagatgttaa aaaccacggg
     1861 tcgccccgtg actcgtggga tcaccttagg cctgaaggat attctcaatg cccgcgaggt
     1921 tttgttattg gtgactggcg aaggaaagca ggatgcgaca gaacgttttc tcacggctaa
     1981 agtctccacc gctatcccgg cttcattttt atggctgcac agtaacttta tttgtttaat
     2041 taatacctaa ccagatgaaa aatttgctca aaatttttaa attaacttat tcaa
//
LOCUS       DAJTYL010000251         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYL010000251 DAJTYL010000000
VERSION     DAJTYL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.258"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..799
                     /locus_tag="OO572_004025"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO572_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO572_004026"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO572_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO572_004027"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO572_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          438 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DAJTYL010000252         2068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTYL010000252 DAJTYL010000000
VERSION     DAJTYL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.259"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..564)
                     /gene="yehT"
                     /locus_tag="OO572_004028"
     CDS             complement(<1..564)
                     /gene="yehT"
                     /locus_tag="OO572_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6662811.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPL"
     gene            complement(561..>2068)
                     /gene="btsS"
                     /locus_tag="OO572_004029"
     CDS             complement(561..>2068)
                     /gene="btsS"
                     /locus_tag="OO572_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6662812.1"
                     /translation="GLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSMGG
                     MTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIA
                     RPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTE
                     GILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYT
                     LKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLF
                     SSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAV
                     IRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNI
                     AIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGL
                     YQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          506 a          570 c          481 g          511 t
ORIGIN      
        1 tagtggtgta cgactttcca aggtacgtaa tgtcaattcg gtaaagccct ctttcccttc
       61 gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg
      121 cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct gttgattttc
      181 cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca gcgttttctc
      241 cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt caaaggcttt
      301 aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt ccgggtcaag
      361 catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc
      421 cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact ctccaacgat
      481 ttcaatatcg ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta acggttcatc
      541 atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg
      601 gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa ccgttcacgt
      661 aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa
      721 ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc gctgattgcc
      781 actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt
      841 tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc
      901 tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac atgttcaatt
      961 tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa
     1021 agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg
     1081 ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt gatttctgac
     1141 tgggtgagca tcgctttttg ccgctcatat tgccctgcaa gaatctgcgc cgaaagcagt
     1201 tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaccggtt tttggcttca
     1261 tacaatttga tggtgcccat cacgcgctga ttttcgccac gcaacggaat taccagcgtc
     1321 gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt gccatcagcg
     1381 tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat cggtttgccg
     1441 ggtaaatggt ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc
     1501 gcgactgcac caatatccag ctcctgatac agtacctgag ccactttcat gctgttcact
     1561 tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag cgcagtggca
     1621 gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac
     1681 agcgccgcgc cgacggtatt ggtgaccatc attggcgcag caatattact caccagacgc
     1741 accgcatctt cataaggtcg ggcgatcgca aggatgatca gcatttgcac catttcagcg
     1801 acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg
     1861 atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggttga gatcatgcaa
     1921 cttagcgcgg tcatgccccc catcgaatat cgatgtaagc cgccggtcag accaaccagc
     1981 ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg
     2041 gcaatagaat cgtcaatgtg caacccaa
//
LOCUS       DAJTYL010000253         2067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYL010000253 DAJTYL010000000
VERSION     DAJTYL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.260"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(63..332)
                     /locus_tag="OO572_004030"
     CDS             complement(63..332)
                     /locus_tag="OO572_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6662813.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            389..1057
                     /locus_tag="OO572_004031"
     CDS             389..1057
                     /locus_tag="OO572_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6662814.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1373..>2067
                     /locus_tag="OO572_004032"
     CDS             1373..>2067
                     /locus_tag="OO572_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6662815.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          624 a          455 c          383 g          605 t
ORIGIN      
        1 aagtttacca gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt tttctttttc
       61 gcttacattg tgtctggttc ttccggccac tcaatatcag gtgcagttga tgtatcaaca
      121 cggttcagca acacccgata cttcttccag gcttccagca acgagttttc ttcctccgtt
      181 gcgatctcca gatctacagc atcctgaagt ggcgcaatat gctcactgaa tccctggatg
      241 tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga agcataccag
      301 gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca tcgccataat
      361 tgatttaatt cacaaataaa actataatat ggtgaaatta atgaaaaaaa acacagatga
      421 tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg ttttgggagt
      481 atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga
      541 acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc ttactcacgt
      601 acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc
      661 tacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgaacctta
      721 tcctgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc ttcactgcct
      781 gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg ccttaactga
      841 tgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca atagcttcgg
      901 tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa aagattccga
      961 agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca aggttcaagg
     1021 tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag cacgttcttg
     1081 cttaagacgt gctacggttt cctttacgat ttggggttgc gactttacct ctattgataa
     1141 tgcattccgg cagaacgttc aaatatgaac gtacgatatt taactaaccg aaaaacaaaa
     1201 tatagcacag gcgagacatt taccatctaa gaaaatagta tcgttttttt acagttaaat
     1261 caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt ttatttcagc
     1321 ttattattcc ccagtatcac attttatcag gatatccttc tgcgaggtta ttatgcttcc
     1381 tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta catcaatcga
     1441 caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca gcatttccgg
     1501 agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggagaaca gtgaaacatc
     1561 gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca atcttgatga
     1621 aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg cattgaaaga
     1681 gctgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt cgctggaata
     1741 tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt cactagaata
     1801 tattaatgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt cattggaatt
     1861 acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa gagatgatca
     1921 tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg aaagcatact
     1981 tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc ccagaataat
     2041 gcaaactttg ttacaacaaa ccgccca
//
LOCUS       DAJTYL010000254         2061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYL010000254 DAJTYL010000000
VERSION     DAJTYL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.261"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(91..>2061)
                     /locus_tag="OO572_004033"
     CDS             complement(91..>2061)
                     /locus_tag="OO572_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6662816.1"
                     /translation="SQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGRAEYNSISTWI
                     QRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFAPVVRGIAKSN
                     ATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLA
                     ILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALG
                     VGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRYSTE
                     GYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDV
                     ERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSGWWG
                     NSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGS
                     YGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAARLEIDNM
                     RGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFV
                     THVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLS
                     WGENASTKCIFNYSFSTPESESGLIEQGVTCH"
BASE COUNT          535 a          528 c          443 g          555 t
ORIGIN      
        1 cctttaggag ctgcaagtgc tacactactt aacgagagca taaacacagc aaagcaccac
       61 tgtttgatat acttgttcat aaaaatcctt ttaatgacat gtcacaccct gttcaattaa
      121 tccgctctca ctttctggtg tcgaaaatga ataattgaag atgcattttg ttgaggcgtt
      181 ttctccccag gataacgtaa cctgtccttt ttctggtaga ccagaaagat aaagttcgcc
      241 acggtcaccg acaattcctg aatctgccaa acttgcatct tgtatcgcag caatggctcc
      301 aaaaggtacc ggtttaccat ttgcatttaa cactctgaag agaacgcgat aacccacatg
      361 agtcacaaac tccgctttaa ccactgcacc acgcgtggga accatcgtaa cgacggtatt
      421 atcaagttct atattcgact tcgagaaata gttatcgctg attgctaccc gattttcatc
      481 ataagggttt agctgtgtct tgaccgtata gccgcgccag tccgtcgcag caccgcgcat
      541 attatctatt tctaacctgg cggccccagg tgctttaaca agagcaatag tttcacctaa
      601 ctcctgactt aacgttaccc cttcgctgtg tgccagaatt ccccctctga taccataatt
      661 cagcctctgg gaattttggc tgtagttata gctgccgttc accgtgccat aagagccatc
      721 ataggtcagg gacatattac cgccaacctc atcatcatga ctgttatagg actgcattaa
      781 attccatgaa aggttatttc tttccagcgc cgtaccgctg agtccgagtt gatgtgagct
      841 accggaatta tcgttttgcg aaacagaata ggtggcgtaa ctattacccc accagcccga
      901 taaaggaaca gataaagaga gcatgtagat ctgctcactg gtgttatcac tggcatggtt
      961 gttccccgtc ccctgtctgg cagtattgga ataactccag gagacgttcc acgagagacg
     1021 cttccagcta ctgctgtagc caaattgcat taatcgttcg acatcatcgg tatgccagta
     1081 ttgttgccgg cttaatgtca ggtataaatt gccataatct ctccccaacg actgcgttag
     1141 cgttccctcc acgcgactac gtcggttacc tgtttcccaa aagtcttcag ggctgttgcg
     1201 ccgcgatgcc cactcattga gggtatagta gccctcggtg gagtaacgat agccaaccag
     1261 gcgcaagcta gtgtctgtgt cgtcaaatag ttttgaatag agaaagcgat atgactgacc
     1321 cgtttctgta tcatcatggc taaaattcgc acgagcatgt gtcacatcga aagaaattgc
     1381 gcccaaagcg ccaagatctt taccgacacc cagagctgca gaaccataat tttcagcagc
     1441 ctgcatacct ccatataaag tgatcccgtg ggaaaaacca tgaagtattt gcgcctgaag
     1501 gacgtccggt gtaaatccgt cttcatcgcg caattcaccc acgctgacat caacatctgt
     1561 cagaccttca cgtttaagaa tcgccaatga agcataaggt tgggtgaatc gttgttcgct
     1621 accgtcttct tctttgatgg ttacgtccaa atcgccacct gtacttgcgg tgttgagatc
     1681 ggtgatctca aaagcacctt gtggaacggc tgactgataa atcacgtagc cattctgccg
     1741 aatgatgacg gtggcgttgg actttgcaat cccacgaacc acaggagcaa agccattctg
     1801 gctggcgggt agcatatcgt tatcagtata caaacgcgcg ccgcgaattt gcgtactgtc
     1861 gaaaatatcg ctcgccgtcc acgtatcacc aatcataatc tggcttctta acgcggcaat
     1921 atcgcgctgt atccaggtac taatggagtt gtattccgca cggccatcgc tggtgtttaa
     1981 agtactgtaa ttacgtagcc gccacggtcc aatattcagt ccattgcgca gattgagata
     2041 actggtatct gtctcttgtg a
//
LOCUS       DAJTYL010000255         1975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYL010000255 DAJTYL010000000
VERSION     DAJTYL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.262"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..199
                     /locus_tag="OO572_004034"
     CDS             <1..199
                     /locus_tag="OO572_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6662817.1"
                     /translation="ARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGH
                     WLDLTFMQLQLDERTEPDAIG"
     gene            196..645
                     /locus_tag="OO572_004035"
     CDS             196..645
                     /locus_tag="OO572_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6662818.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(646..879)
                     /locus_tag="OO572_004036"
     CDS             complement(646..879)
                     /locus_tag="OO572_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6662819.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(965..1138)
                     /gene="ortT"
                     /locus_tag="OO572_004037"
     CDS             complement(965..1138)
                     /gene="ortT"
                     /locus_tag="OO572_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6662820.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1333..1428
                     /gene="yncL"
                     /locus_tag="OO572_004038"
     CDS             1333..1428
                     /gene="yncL"
                     /locus_tag="OO572_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6662821.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1830..>1975)
                     /locus_tag="OO572_004039"
     CDS             complement(1830..>1975)
                     /locus_tag="OO572_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6662822.1"
                     /translation="NYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVF
                     RGL"
BASE COUNT          505 a          447 c          444 g          579 t
ORIGIN      
        1 agcgcgggat tgcgggaagc atgtcatggt cgccgggatc gaatcgcaaa atcaggcttc
       61 gctgcatctg caccagtcgc tgggatttgt cgtcaccgca caaatgccgc aggtaggcac
      121 taaatttggt cattggctgg atctgacatt tatgcagttg caactcgacg agcgcactga
      181 accggacgcg attggatgaa tcagtcgctc acccttgctt ttttgattgc cgccggaatt
      241 ggtctggtag tccagaacac gctgatggcg cgcatcaccc agacctcctc taccattctc
      301 atcgccatgt tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt
      361 aaacaaggca tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata
      421 cctggcttgc tgggttcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga
      481 gcggcgacaa ctattgcggt actggtcgcc agccagctga ttggcggtct ggtgctggat
      541 atcttccgca gtcatggcgt accactacgg gcgttgttcg gcccaatatg tggcgcaatt
      601 ttacttgtgg ttggcgcctg gttggtggcc agacgctcat tttaatcaga ggatggtgcc
      661 gcctttggtt aactgttcgt ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg
      721 agcaatagcg gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc
      781 gtcatcgctc agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc
      841 ggcatccacg cgcgcgatga cttcatccag atgcgacata ctttctcctt agccacttgt
      901 gaaatttatc gattaatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta
      961 agatttaaaa cagtgaaaac aacagcgcca ccgggaaact catcggccat gtggccccca
     1021 ccagaaaggc gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caggtgataa
     1081 gaaatgcgat tgctgccata accgcataaa aaacaagcat atgttgatag agaaacatgt
     1141 acgggcgtcc agccatatga ataaaacgcg cgcaaatatg cgccattttt tgacgtgcat
     1201 caagtttttt caccagtttg agaaaaaatc atcaaacagc aacttgccct tatccatctt
     1261 cagaatgagt attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa
     1321 aaggtggcaa aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct
     1381 gttggtttgt ttactcttat ctctatgaga tttggctggt ttatttgatg tagtgatttt
     1441 gcacgcagca cgtactgtca gagaaatcaa ctctctgaca gtacgcaaga tcttctcata
     1501 ctctccaagt ttgcaacatt atccttaata tttacttctt tttgctggca tctacattga
     1561 aatgagtaga aaacatcccc ctactgtcgt ctatacaaac agcttactga ttcaaacgga
     1621 atcttgtttt cacatttttc tatcacgaat actttatacg cgcgggagtc tataaatgct
     1681 ttatactttc aaggaataac gtaaatataa aaagatggcg taaagtatat gcatggcagg
     1741 atgtttgcaa cctccttgat gacgctgccc cattgaaatt gcaaaccccg ccctggttat
     1801 cttgcccatt tatcatgcaa aacagataat tataggcctc tgaatacctc agaaagattg
     1861 aattttttat agtccctctc ttgaatattt ccatttgata tcaatcgatt catctcttca
     1921 acaaaataat ttatatcatc acacttatta ttatccggaa gatctgagta gttat
//
LOCUS       DAJTYL010000256         1970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYL010000256 DAJTYL010000000
VERSION     DAJTYL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.263"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            100..795
                     /locus_tag="OO572_004040"
     CDS             100..795
                     /locus_tag="OO572_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6662823.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            824..>1970
                     /locus_tag="OO572_004041"
     CDS             824..>1970
                     /locus_tag="OO572_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6662824.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSD"
BASE COUNT          583 a          468 c          431 g          488 t
ORIGIN      
        1 taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat aacgcccttt
       61 tccttcctct ttctcgttgt atcaggttga aaaataacta tgtcaaaacg aacattcgcg
      121 gtgatattaa ccttgttgtg tagcttctgt atcggccagg cgcttgcagg gggtatcgtt
      181 ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt acctgtcgca
      241 aacaaaggcg cagaaacgcc ttatttactg caatcatggg tagataatat agatggtaaa
      301 agccgtgccc catttattat aaccccaccg ctatttcgtc ttgaggctag cgatgactca
      361 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      421 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      481 acgctggtat ttaaaacgcg ggtcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      541 gtaaacgatg cgtggaaatc actggaattt aaacgtagtg accattcact caatatatat
      601 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      661 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc agcatctggc
      721 ggaaagaacg tgaaatgggc tgcgatcaac gattatggcg gcagttccgg gacagaaact
      781 cgtccactgc aataaaaaat ataaaaacac aggccatcag ggaatgccac aacgacacca
      841 ccagggacat aaacgcacac cgaaacagtt ggcgctcatt atcaaacgtt gtttgccgat
      901 ggtgctcact ggcagcggca tgctttgcac taccgctaac gccgaagagt attatttcga
      961 ccccattatg ctggaaacta caaaaagtgg tatgcaaaca accgatctgt cacgtttttc
     1021 aaaaaaatac gcacaactac caggaactta tcaggttgat atctggctga ataaaaagaa
     1081 ggtttcacag aaaaaaatta catttaccgc caatgcagag caacttctgc agccacagtt
     1141 tacggtagaa caactacgtg agctgggtat taaggtggat gaaatcccgg cgctggctga
     1201 aaaagatgac gatagcgtga tcaactcgct tgaacaaatc attcccggta cagctgctga
     1261 atttgatttc aatcatcagc gacttaattt gagcattccc caaattgcac tgtaccgtga
     1321 tgcaagaggt tacgtctccc cttctcgttg ggacgatggt ataccaacgc tgtttaccaa
     1381 ctactcgttt acaggttctg ataaccgtta ccgccagggc aatcgtagcc aacgacagta
     1441 cctgaatatg caaaatggtg ctaattttgg cccctggcga ttacgtaact attctacgtg
     1501 gacacgcaac gatcaggcgt caagctggaa cactatcagt agttatttac aacgtgatat
     1561 caaggcgttg aagtctcagt tgcttctggg agaaagcgcc accagcggca gtattttttc
     1621 cagctacgcc tttactggcg tgcaactcgc ttccgacgat aatatgttgc caaacagcca
     1681 gcgcggattt gccccaacgg tacgcggtat cgcaaacagt agtgcaatcg tgactatcag
     1741 gcaaaatggt tatgtgatct atcaaagcaa cgtgccagcg ggtgcctttg aaattaacga
     1801 tctctacccc tcttccaaca gcggcgattt agaagtcacg attgaagaaa gtgacggtac
     1861 acaacgtcgc tttatccagc cttattcttc attacccatg atgcagcgac ctgggcatct
     1921 aaaatatagc gcgaccgctg gacgctatcg cgctgatgca aacagtgata
//
LOCUS       DAJTYL010000257         1963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYL010000257 DAJTYL010000000
VERSION     DAJTYL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.264"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(150..314)
                     /locus_tag="OO572_004042"
     CDS             complement(150..314)
                     /locus_tag="OO572_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662825.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1216..1500
                     /locus_tag="OO572_004043"
     CDS             1216..1500
                     /locus_tag="OO572_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6662826.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1488..>1963
                     /locus_tag="OO572_004044"
     CDS             1488..>1963
                     /locus_tag="OO572_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6662827.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKL"
BASE COUNT          503 a          413 c          565 g          482 t
ORIGIN      
        1 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
       61 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      121 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      181 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      241 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      301 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      361 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      421 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      481 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      541 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      601 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      661 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      721 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      781 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      841 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      901 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
      961 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1021 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1081 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1141 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1201 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1261 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1321 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1381 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1441 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1501 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1561 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1621 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1681 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1741 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1801 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1861 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1921 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa act
//
LOCUS       DAJTYL010000258         1929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYL010000258 DAJTYL010000000
VERSION     DAJTYL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.265"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            274..578
                     /locus_tag="OO572_004045"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO572_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..>1929
                     /locus_tag="OO572_004046"
     CDS             747..>1929
                     /locus_tag="OO572_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6662828.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          508 a          380 c          439 g          602 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcat
//
LOCUS       DAJTYL010000259         1883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYL010000259 DAJTYL010000000
VERSION     DAJTYL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.266"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(104..1558)
                     /gene="ospC2"
                     /locus_tag="OO572_004047"
     CDS             complement(104..1558)
                     /gene="ospC2"
                     /locus_tag="OO572_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6662829.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          599 a          318 c          292 g          674 t
ORIGIN      
        1 caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa tataacaaaa
       61 atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc ccaaatcgtt
      121 tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct ttgcttttca
      181 ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg tttacatcaa
      241 ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct tcactaagta
      301 aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc atctctgcga
      361 tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca gattttgcat
      421 tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc ttattgttaa
      481 tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg agtcttatct
      541 ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga aacacaaagt
      601 ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac tctcggaatc
      661 tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca agcctcatat
      721 ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca tacctgctaa
      781 cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca tggtaaaaaa
      841 caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat ggagagtcat
      901 gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta tttagtggga
      961 gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg ttagctattt
     1021 cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac ttaattttat
     1081 caaaggactt aatattaaga gtattatttg acaggttagc gttactttga tgtctgaagt
     1141 taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa agaggatgag
     1201 ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc tcaagtgacc
     1261 gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat ttgaaactgg
     1321 tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt aaaaagcttt
     1381 tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag gatgagtttg
     1441 ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt atttttccat
     1501 caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt attttcataa
     1561 aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt atctaactta
     1621 atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat tttcactctg
     1681 aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa cagtattatt
     1741 tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat atgatagata
     1801 aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa aattagtaac
     1861 aataaatgaa agaatcatgg gcg
//
LOCUS       DAJTYL010000260         1880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYL010000260 DAJTYL010000000
VERSION     DAJTYL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.267"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            3..830
                     /locus_tag="OO572_004048"
     CDS             3..830
                     /locus_tag="OO572_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6662830.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            874..1623
                     /locus_tag="OO572_004049"
     CDS             874..1623
                     /locus_tag="OO572_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6662831.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1620..>1880
                     /locus_tag="OO572_004050"
     CDS             1620..>1880
                     /locus_tag="OO572_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662832.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQ"
BASE COUNT          518 a          483 c          492 g          387 t
ORIGIN      
        1 agatgaaaaa agttattcct gctttagccc ttgttctgct gaccaccgga cttgtcggtt
       61 gcgatcgcgt tgagcctggt aatgttggca tcaaggtaaa caaactgggc gacgataaag
      121 gcgtcggaga agtggtcggt gttgggcgct actggacggg atggaacact gaggtttaca
      181 tcttcccgac cttcaaacaa atgaagacct acgatgagcc attcagcttc cagatgagtg
      241 acggtacaac catcggctat cacatcggcg tggcctacaa agttgatcca tccaaagtta
      301 ccacagtctt tcagacctac cgcaaaggcg tggacgacat taccgacacc gacctgcgcc
      361 agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgac aaatttatcg
      421 acggcggcaa gtctgagctg ctggatgcag ctcttaaaga cattcaggaa gaaatgacgc
      481 ccatcggtat tcaggtaatg agcctctcat atgtgggtaa gccggagtac ccgcctactg
      541 ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa aaccctgcaa tgcgagcagg
      601 aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc ggaagctgcc ggacaggcag
      661 atgcgattcg cacaaaagcc caggccgaag ccgatgctat tcgtttacgc ggtgaagctc
      721 tgcgccagaa cccaaacgtt atggagctgg aagccatcaa caagtggaac ggtacactgc
      781 cgcaatacat gaccagcggt gccaacacac catttattca ggttaagtaa tacatatgcc
      841 cggtattaca cgccgggctg tctggagata aaaatgaata ctgtaaccat caacaacaaa
      901 cagtttccgg taatcgaata tcgcggtcag cgtgttgtga cgctggcgat gattgatgaa
      961 gtccaccagc gaccggaagg aaccgcgcgt gcagcattca accgaaaccg tgagcatttc
     1021 atcagcggtg tggattatgc cgaattaggt gcggacgtaa tacgtacgga cctcccggaa
     1081 ggaacattct ctaaatttgc accatcgggg attgtacttt ttgaatcagg ttacctgatg
     1141 ttgacgaagc cattcaatga cgcccttgcc tggcaggttc agcgcgaact ggttaacagc
     1201 tacttccgca ctcgcacacc gctaacggaa atcgagatga tcgctgcgat agccgccgac
     1261 gctgttcgtc aacagaagcg cctgaatcat gtggaagaac agctcgaaac agtcacagaa
     1321 gcggtggaaa ccatcaaacg cggaaatatg cgcgccggat atgtcggtta ccgccaggtg
     1381 gtagccaaaa gtggaatgag tgacaccaag tgtcggaatc tggtgaacgc atatcaaatt
     1441 cctacagaca cccacgaatt catgactccg gacggtctgt tgtctcgcag ggctatcgtc
     1501 gagcttgagc catttatggc ggcgttccac caaatgatgt cagaggctga cccacgcggc
     1561 acacgctggt atcacccgaa gatggggctt ttccaggtga tcggatggga ggataaagca
     1621 tgatcatcca gtcaaaactt attcgcgccg ctctggtgtg cgctgctaaa aacgacgttc
     1681 gttattacct gaacggtctt cacatcacgc cgaaacatat tgaggcaacc aatggttccg
     1741 tggcactgcg catggctcac ggcatccgga cgaagaaaaa catcattgtc cagttcgaag
     1801 ggggcgtccc ggccaaagcc gaaacgacag agctgatttt tagtaaagag ccgatcgctg
     1861 ttcatcgcga ccagtttcag
//
LOCUS       DAJTYL010000261         1811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYL010000261 DAJTYL010000000
VERSION     DAJTYL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.268"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..389)
                     /locus_tag="OO572_004051"
     CDS             complement(<1..389)
                     /locus_tag="OO572_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662833.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWL"
     gene            complement(1051..>1811)
                     /locus_tag="OO572_004052"
     CDS             complement(1051..>1811)
                     /locus_tag="OO572_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662834.1"
                     /translation="IIAHTLADRSVLVPVRGGKRPEVVTAPSGTEITVEPIEQAILVS
                     HQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMVDVLESSKLLKTVELPALTG
                     SLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKALSRLAAKQGFGGEDAIVDM
                     LGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITVVLSRDGDSQSHDLEILGKV
                     FVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          507 a          410 c          421 g          473 t
ORIGIN      
        1 ctcagccaga cagcatcata gtgggtttct gccgctgttt tgtcatcttc gaaagtagaa
       61 gcaagaataa acgttcccca gggtttctcc gtcaacgtta actttcgtgt gttttctgtt
      121 aatgttagca atccttgagt ttcgtctttg gtatagtgac tctttatttc attttttaat
      181 ttggctgagt gaatcagaag acgataatta ggatacttat ctggatttaa cgataaataa
      241 cttttatctg ttgctgcccc cctggaatta agaatgttaa atacagattc gcttgtcact
      301 tctgcctctg tcgcctggca tgtgccgcac gaaaatatac cagcgagcag aagagttaat
      361 gcagatttcc ctttcataaa ataatccatc aatcgtttgt taaaagcgta gataaccact
      421 tattcaacaa cttgaaaata aggtatttta cctgagttgt tacaaaagga ttgcattgcg
      481 taaacgcttt ttatttacaa aaaaatgggg aagtgttaca gcgattgcga aaagcctgaa
      541 gtgattgaat taagataata tagaaaatca gatacagcaa aaatggcaga ttgatcaggt
      601 gaagataaat tggagcgggc gaagggagcc tacgacaaat cgaagacaac cagctaacct
      661 attgtttctt aaagacaggt gaatccagtt ggttgtagtg atagtcacac atttagagat
      721 agaaggtcaa gacttgaagc acgtaatatt ccctctaaag gaatagaact cacggtctga
      781 ctacccggcc cttttggtct ggctcaggtc aggctatggg ggcttttggc tcgctcttta
      841 tattagatga ggtctcacat ttttcagtca aaactcatcg tctaatagcc catagccaga
      901 ccaccagtcc cagctttagc aggaagacca cgagatagac tttgtgtagg tccatttggc
      961 cctcctactt tctgtattca caaactagat tactagtaca aaataaaggc cagacatcca
     1021 gttcggaagt ctgaccaata gtcgttattg ttagttagct tctttagtga agctcaggcg
     1081 tttgactaca ggcaggacag cagtagccga gccagtaccc atcttgatgg tcgtagcagc
     1141 ttcagctaca acaaacacct tgccgagaat ctcaaggtcg tgagactggg agtctccatc
     1201 acgtgaaagg accacagtaa tgctctcgcc atcgtgacgg aagcaaccga agctcagagc
     1261 cgtaaaacgc ttagccatca tgaacacgcc acggtcttca ccgttgtaat aggcgacgtc
     1321 agtccccagc atatcgacga tagcgtcttc accaccgaag ccctgcttag cagccagacg
     1381 ggagagagcc ttgaggtgtg actcatgggc gataatgccg tagtcagaaa cctcagaacc
     1441 gaaagaagac tccgtgttct cgtacagggc atccatgata gcgtcagcct tagcggtcag
     1501 gctaccagtc agggccggaa gttctacggt cttcagcagc tttgaggact ccagcacgtc
     1561 aaccatctta gagttaatgg tacgaatcac gttatcgcag atgttcagtg cgtcctgctg
     1621 gagcgtcccc tgagtgaaac cagtggtcga cttctggtcc caaagctttg tttggtgaga
     1681 cacgaggatc gcctgttcaa tcggctcaac ggtgatttca gtgccagacg gagcggtcac
     1741 aacttcaggt cgcttaccac cacggacagg gaccagcaca gagcggtcag ccagagtgtg
     1801 agcaatgatt g
//
LOCUS       DAJTYL010000262         1802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYL010000262 DAJTYL010000000
VERSION     DAJTYL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.269"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..609)
                     /locus_tag="OO572_004053"
     CDS             complement(<1..609)
                     /locus_tag="OO572_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662835.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGDTGTG"
     gene            complement(687..1109)
                     /locus_tag="OO572_004054"
     CDS             complement(687..1109)
                     /locus_tag="OO572_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662836.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(1093..1320)
                     /locus_tag="OO572_004055"
     CDS             complement(1093..1320)
                     /locus_tag="OO572_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6662837.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1397..>1802
                     /locus_tag="OO572_004056"
     CDS             1397..>1802
                     /locus_tag="OO572_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6662838.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          460 a          480 c          382 g          480 t
ORIGIN      
        1 tccagttccc gtatcgccct gctccgttcg aatcggtttt cgcaatgcct gtctgaaccg
       61 gacccactca tgccagagtg tttccggcaa ccaatcgggc agctcaatag cctccggctc
      121 gaatttttta gacgctcgtt tttggcgagg gggatttagg gggagatcag tatttagatc
      181 ttcctcttcc tcttccactg gtaacgcttt ttgatccgtt tgtgtaacgc tggcagcgtt
      241 accttttcgt ttcagttcgc gtatttttgt tactcgctcg tttgtaaccg cccgtttttt
      301 agagcttttt ccgttatgac gttcaaagtt aggtagagaa agcccaccgt cattttcgac
      361 cagccatcca acctgaatta acgcatcagc aaaaccagac ataaaagtga tgcgatctat
      421 tgcacttttt gtaacgccgc gagcgttaca atctgcatta ccgtctatca tttgttgatc
      481 cgcccatgcc cagaagcgaa taaccttccc taatgcggca tctggatcaa tattcagaat
      541 ctcagcaagc ctgaatattt ccggcttatc cggcgtaata acctcgagct ttatccagtt
      601 tgaagccatt tgttttcacc ttgtaacgct cgcagcgtta catttaactg ataccgaaca
      661 aaacaatccg gcacgattaa tttcaatcaa tgcactacga cagaatcgcc gggcgaccca
      721 ccaccgctga aatgtgcttt ccggtaaacg gcctggacag catcatcatg cgcatcaatt
      781 gccgtactca acgcttcctg cgccgccagt aatgcacggc gttccagggt atcgaagata
      841 cagagtcggt gacgcagctc acgcggaaga attgccagaa ccgcagggat cagtttctga
      901 attttttccc tttgcgcttt cgtttcacct ttcaaccaac ggtgatagat attctgctga
      961 ttgttccagt ccttgcctgg taccagagac aattcgccgc ccccctgccg cagatattct
     1021 tcagtaattg cgttagcgac ccacgcctgc cctttttcgg ctgccagggc taacagcact
     1081 gattcgatgt gctcatgcct gattttcatg aatcaccttc ccaggtcgtt ttgccttacg
     1141 atattcgtca taaactttgg ggtcgtactg aagttccccg ccagatgcct cttgcaggcg
     1201 catcgcgcga ccttcaggaa ccagttcccc ccatgcagca atgcttgcca gcctaactcc
     1261 tgcggcattg gcaagctttg ttttgctgcc aaaaaacgct atagcatcaa ttttcaacat
     1321 atcgagctcc ttagattttc ctaaggaaac caggccgtag agaaacctaa gtcaagaaaa
     1381 attagaattc cctaatatga aaaacgaaac cttcggtgct cgcctcttac ataggcgtaa
     1441 aaaattaaaa ctgtctcagg ccgcattagg taagctggtc aaagtggctc acgtaacaat
     1501 ttctcaatgg gaaagagatg aaacacagcc ggcggggaaa agattattcg cactgagcca
     1561 agcgcttcag tgctcaccga cttggcttct ttttggggat gaagataaac aaccaggcga
     1621 accgatcccg gataatcagc cagccattct gacagaagat caaaaagagt tacttcaact
     1681 gttcgacgca ctgcctgagt cagagcaaaa ggccctgttg tcagagatgc gtgctcgagt
     1741 tgaaaatttc aacaaacttt ttgaagaact actcaaagct cgcaaaagaa gcgcaaacaa
     1801 at
//
LOCUS       DAJTYL010000263         1799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYL010000263 DAJTYL010000000
VERSION     DAJTYL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.270"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1310)
                     /gene="ospC4"
                     /locus_tag="OO572_004057"
     CDS             complement(<1..1310)
                     /gene="ospC4"
                     /locus_tag="OO572_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6662839.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNIILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFIN"
BASE COUNT          539 a          313 c          298 g          649 t
ORIGIN      
        1 ttattgataa aatattctag cactttatag ttggcaccat ctttactaag taagtattca
       61 acatcataca gattacaata tctattgttt ttcattttct ccatctctgc gacatcttgt
      121 tttgtaaaat taaatttaga tagtaaataa agtgctatat cagctttttt attgcctaaa
      181 gaataagcaa gggcctgatg agccattttt ttatcagtca ctttattgtt aatctcttca
      241 taattagatg ctttaatcgc atcctccaaa gatatgtctc tgagttttat ttttttgaag
      301 ttatctgtac ttagcattct tggtatatga tactcaagct gaaacacaaa gtttataatt
      361 ctatccagac taacaggatc aatatttttg ttataacaaa actctctgaa tccttgatct
      421 ttactctttc ttataaaatc aataagatgc aacccaattc caagcctcat atctttagtg
      481 ttaaatattg gaacatcagt tttcccctga ttaccatgca catacctgct aacttcatcc
      541 acaacctctt taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga
      601 atagcattat caaagtgatc ggttaatgtc atatatccat atggagagtc atgatcaatg
      661 atatacgcat tggcgccaaa atctacagta tgatgtgttt tatttagtgg gagtttttct
      721 tgatgattgg aaatttcgac cccaaaaaaa acaaaatcgt ggttagagat ttctttaata
      781 tcctcagagt atgtattctt atgtgtttgt atattttcag actgaatttt atcaaaggat
      841 ttaatattaa gaataatatt tgacagatta gagttacttt gatgtctgaa gttaaaatta
      901 gaattaataa taatagaaag taagtgacgt tcgtctgaag aaagaggatg aactttttgt
      961 attatctcag agatatagtg cctggacacc ttatcgagat gctcaagtga ccgaaatgat
     1021 gaccgtgctc cagatggcgc ttcaagagaa agatttagtg atttgaaact ggtcccttga
     1081 gaaaacattt tgctataact ttgcgctgct atagacttcc ttaaaaagtt tttcacagta
     1141 ttagcacagt gatctaaatg tttttcactt atgccagaag aagatgagtt tgttacgcgt
     1201 aatacatttt tttgtaatgc ttttgttgct ttattcggat tcgtttttcc atcaacaaga
     1261 tcaatattat tctgaacatt aatatgattt actgcttcag gtattttcat cccccccccc
     1321 caattatgtg cgtttttatc tgtggttatt tagataacat tatctaactg gacactttct
     1381 tgtatgtatt atagctaaat gaacagattt taatatgttg tatggttttc actctgaaat
     1441 cccctgaatt taatcacgtt tcctttactg aaggcaattt taaccgtagt atcaattatt
     1501 aacccttggg cttgatatcc ctctcctctt ctgggcacgg gggatgtcag attacttatt
     1561 acagaaacca aaagaattgt aagcgtcatc tcagtgtcgt ctatttcaat gtctgatgat
     1621 tggcgatgat agagtcctct tataacgtta atggactcta tcaatgtcag attctcttcg
     1681 cccccgcagg attcgtgcct cttactctat gactctatgg agtttaagct tgatcttatt
     1741 gaaaaatctt accagctagg ggcctgcgtg gcccaactgg ccagagagta cgggattaa
//
LOCUS       DAJTYL010000264         1719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYL010000264 DAJTYL010000000
VERSION     DAJTYL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.271"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            114..1718
                     /locus_tag="OO572_004058"
     CDS             114..1718
                     /locus_tag="OO572_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6662840.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          470 a          394 c          437 g          418 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctaga
//
LOCUS       DAJTYL010000265         1642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYL010000265 DAJTYL010000000
VERSION     DAJTYL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.272"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            6..1031
                     /locus_tag="OO572_004059"
     CDS             6..1031
                     /locus_tag="OO572_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6662841.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1099..1642
                     /locus_tag="OO572_004060"
                     /pseudo
     CDS             1099..1642
                     /locus_tag="OO572_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          376 a          380 c          427 g          459 t
ORIGIN      
        1 gcattatgaa aaagatgatg atagccacac tggctgccgc cagcgtgctg cttgccgttg
       61 caaatcaggc gcatgctggc gcgacgcttg atgccgttca gaaaaaaggt tttgtgcaat
      121 gcgggatcag tgatggatta cctaggttct cttatgccga tgcagacggt aagttttcag
      181 gtattgatgt tgatgtttgt cgtggtgttg ccgctgctgt atttggtgac gacacgaaag
      241 tgaaatatac cccactcaca gcaaaagaac gcttcaccgc tttacagtca ggggaagtgg
      301 atttgctctc ccgtaatacg acctggactt catctcgcga tgccgggatg ggaatggcat
      361 ttaccggcgt cacttattac gacggcattg gcttcctgac gcacgataaa gcggggctaa
      421 aaagcgcgaa agaactggat ggcgctaccg tctgtattca ggcgggtact gataccgaac
      481 tcaacgtcgc cgactatttc aaggcaaaca atatgaagta cacaccagtg actttcgatc
      541 gctctgacga atcagcgaag gcactggaat ctggtcgctg cgatacgctg gcttcggatc
      601 agtcgcaact gtatgccttg cgcatcaaat taagcaaccc ggctgaatgg attgtcttac
      661 cggaggttat ctctaaagaa ccgcttgggc cggtagttcg tcgtggcgat gatgaatggt
      721 tctcgattgt acgctggacg cttttcgcca tgctaaatgc tgaagagatg ggcatcaatt
      781 cccagaacgt cgatgaaaaa gcggctaatc cagcaacgcc tgatatggca catctgttgg
      841 gtaaagaagg cgattacggc aaggatctga agttggataa taaatgggcc tataacatca
      901 tcaaacaggt gagtaactac tcggaaattt ttgagcgtaa cgtaggttca gaaagcccgc
      961 tgaaaattaa acgtgggcaa aataatctct ggaataacgg cggtattcag tacgcgccgc
     1021 ccgtgcgtta agtcggtgtg aggtaacgag cgtcgctacg gcggcgctct cttcagcgtt
     1081 atgctttccg aggtttctat gcctcatcgc cgctcaaccg ttaaaggctc actctccttt
     1141 gccaacccta cggttcgtgc ctggttattc cagatccttg ccgttgttgc tgttgtcggc
     1201 attgttggtt ggttatttca aaacactgta acgaatctca ataatcgtgg cattacttca
     1261 ggttttgcct ttctggatcg cggcgctggc ttcggtattg tccagcattt gatcgattac
     1321 cagcagggcg acacctacgg acgcgttttt attgtcggct tactcaatac gctactggtt
     1381 tctgcattgt gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct ggcgagactt
     1441 tcggataact ggctgctacg aaagctttcc acaatttata ttgagatctt ccgtaatatt
     1501 gcaaatcttc ttctggtact ttgccgtgtt gcgcaatttg cccggaccac gccaagctgt
     1561 gagcgcgttt gatctggcct ttttgagcaa tcgtgggctt tatattccgt caccgcagct
     1621 gggagacgga tttattgcgt tt
//
LOCUS       DAJTYL010000266         1635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYL010000266 DAJTYL010000000
VERSION     DAJTYL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.273"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(106..366)
                     /gene="yfhL"
                     /locus_tag="OO572_004061"
     CDS             complement(106..366)
                     /gene="yfhL"
                     /locus_tag="OO572_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6662842.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            581..1635
                     /locus_tag="OO572_004062"
                     /pseudo
     CDS             581..1635
                     /locus_tag="OO572_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          433 a          392 c          379 g          431 t
ORIGIN      
        1 aaattaactt agtgaatgat tatgctaatg tcatcaatta aataaatata atggcgttaa
       61 ggcttcccag taatataatt aatactctac ttccagagta gaatattaaa ttttatccgc
      121 gtggtgcatc agcacaaatt tatcccacaa ctgttcttct gtctcgacat gcgccggatc
      181 tttcacaata gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc
      241 tacgcattcg gtacacttat cgctgttaat ttcgtagata tgatctccca ttgaaatcgc
      301 ctcattcggg cattcgggtt cacacatatc acaattaata cagcgtttag taattagtaa
      361 agacatttca atggattacc gttaaatcat tttaaaatca gtaagttgta tcgagtttgt
      421 atgctttact gtcattaact tactgtatgt tgatccagtg tatttaacct tgataaactc
      481 agtccagcaa cacaaaaccg caacacattg cattttgtcc cgtagaaaag acttgtatgt
      541 gtgagcttgt tttctgcgcc tacgcagata aggattgaga atgccgcgca ctgtaacaca
      601 taatccggat agccccaata atgacgatgt tttagccgca tctgaaaaat gggacgcctg
      661 taaacccccc tataccagcg cacacatgaa aatctgtgtt gctgccgcca aaatcatcct
      721 cgctgcttcc ggcgtggctc gctgttccaa atacgaaaaa gagaactatc tccgtatcga
      781 tttcagcaaa gccggtaagg ttacatttta cgccgagttt ccaaaaaaga tgggcctcaa
      841 aggtaaaaag ctcggcgagt ggccggagct cgttatccag ctggcgcgcg aaaaagcact
      901 aggtatggct gacggtggac tgcgggcaga gtccgtacat gcagcgctgg aaatgtaccg
      961 ggatgaccca aagccaaagt agcccggcag aagctgagcc cggacagttt cacaacctac
     1021 ggggtgcgta tcgaccggat taaagtaacg ttcggcgagc gtgaggtgtt cagcgacgta
     1081 acatacaatc ggctggtgga agtgctggac gagtggatcg ccactcgctc gaacaataac
     1141 gccctggagt tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc tactctttgc
     1201 aacggccgca atgttgccgc cagtctgcca gatgattatg tttcctcccg cgtacagaaa
     1261 cctaccccca cacggctttt taccgatatt gaatcaatcg cccgactctg gctcaatgtt
     1321 gctgcctgca cctctgtaca ccagaagaat gctgttcgct tcatgatcat cactggtgtt
     1381 cgtccgatta atgtccataa cctgcgctgg gactacgttt acgaggaggc tggtgaaatt
     1441 gtttatccgg aaggggttat cggcatgcga ggggctatga aaacacaaaa ggctttccgc
     1501 ctgccgataa cgcctgagat ccggcggatt atcgacgagc agaaagcctg gcgtgattca
     1561 gttcctgagt gcaacaggga ttatgtattt ttgcagccac gtgatccaat gcagccattt
     1621 tcaaaacgat cactg
//
LOCUS       DAJTYL010000267         1602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYL010000267 DAJTYL010000000
VERSION     DAJTYL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.276"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..214
                     /locus_tag="OO572_004063"
     CDS             <1..214
                     /locus_tag="OO572_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6662843.1"
                     /translation="TLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKIIEP
                     VIELYNDVVWASFNTLHNKINPYKES"
     gene            216..581
                     /locus_tag="OO572_004064"
     CDS             216..581
                     /locus_tag="OO572_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6662844.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            578..1243
                     /locus_tag="OO572_004065"
     CDS             578..1243
                     /locus_tag="OO572_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662845.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1243..>1602
                     /locus_tag="OO572_004066"
     CDS             1243..>1602
                     /locus_tag="OO572_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6662846.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVN"
BASE COUNT          439 a          328 c          423 g          412 t
ORIGIN      
        1 tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc tgtttctcat
       61 tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga taatcggaaa
      121 aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat tcaacacact
      181 gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa aaacaacgtg
      241 cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa
      301 agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg cttcaccagg
      361 aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg cacaagcatt
      421 gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt
      481 ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct gacccggttg
      541 tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta ccaaagagcg
      601 actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag acggcggtga
      661 aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg tagcggaatg
      721 cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg gtcactcgct
      781 gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg atggatggaa
      841 agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt
      901 cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc ataaagcatg
      961 tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa
     1021 cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg gctctgatga
     1081 aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga
     1141 gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt atgttgatgg
     1201 ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa tcgaaccaaa
     1261 ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt
     1321 ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg caactctccg
     1381 gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg
     1441 attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt tttttgcccc
     1501 actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt agagagctca
     1561 actcactgga tgccgctacc ggaaccaccg caggaggtga at
//
LOCUS       DAJTYL010000268         1557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYL010000268 DAJTYL010000000
VERSION     DAJTYL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.277"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            193..462
                     /locus_tag="OO572_004067"
                     /pseudo
     CDS             193..462
                     /locus_tag="OO572_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="OO572_004068"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO572_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO572_004069"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO572_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6662847.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          458 a          316 c          372 g          411 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcaacaaggc gaatcacgca cggatttcca gccagagaca cctgaaa
//
LOCUS       DAJTYL010000269         1556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYL010000269 DAJTYL010000000
VERSION     DAJTYL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.278"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..379)
                     /locus_tag="OO572_004070"
     CDS             complement(<1..379)
                     /locus_tag="OO572_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR6662848.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLE"
     gene            complement(330..1094)
                     /locus_tag="OO572_004071"
     CDS             complement(330..1094)
                     /locus_tag="OO572_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662849.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1289..>1556
                     /locus_tag="OO572_004072"
     CDS             1289..>1556
                     /locus_tag="OO572_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6662850.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQ"
BASE COUNT          379 a          396 c          337 g          444 t
ORIGIN      
        1 cttccaacca caacatatcg atcccctcca gtgacttaat ttcttcgatg ttgcgccata
       61 atccataaaa aacaaattcc gagccagtaa cccggttaat gattttgttg ttcagaatac
      121 ggaaacggtg ccgcagacca aacctgtcta tctgaatttt gagcagggta tacaccgact
      181 cttcgatttt attctggatc tgacgggcac aacaaaaacg cagggtgtat ttattcgaca
      241 gaaatatggc aatgccagcg gcatcccatg attttgacga tgaccgacca ccataaagca
      301 ctttgttacg tgcccgcata gtccagaaac tacgtaaagc cggattcagc gtcggtttgg
      361 atgtcagagt agaagtcatt gaggtcacgc tctccattgc catcatcaat acctgcatca
      421 cggcgaagac gatcagcctc cagagacacc ttatcagtgg cggccttgcg gtaggctgta
      481 tcagcaaata tttttcctac cgtcgcaagc gtgccaacga tggactcaat acgaactgta
      541 ttgcgcatca ttgctttctc ggcggcgctg atattttcaa tcagcatctt cctttcctgg
      601 tccccattag catcttccag cgacaccaac caccgaccaa tattctctgc agcgacaagg
      661 ttgttagcac gaaggcgaaa taattcgtcc tcgagcgtca acgctttagc gtcctctatc
      721 acctcatctt tgagcagaag gcgacgggca taaccaccgt gttttaatgc ctgctggtta
      781 ccgggttgga atgggttagt cgggggatcg gtacgcattc cgcgtatcgg tttcgtatcc
      841 ggtgtagatt ctgtttttag ttgcgtactt ttttgtgtaa ggccagtaat ggcaggcttt
      901 ctgctggtac gcactttcct tttttgcgta ccatttttgc aaacctgcgt accgccactg
      961 cgtgcccaat caagcttttt ggccctcttc ctgatagccc cttctgtaac gccgtatttc
     1021 tcgcctatat cacggaggct aaggactccg gcccggtatg ccgattcgat ggcctcccag
     1081 tccggttttg ccatgattat gcttcctgtg atttactgtt atcgctgcct gtcacaatag
     1141 gttgctttaa ttcttcctca tcactcactc ctgcgacgct ccgtggcagg gccccccaca
     1201 ggagtacctt gtaaatgtaa ggtgtttaag cctaaattaa gtcgtatcac ggttaaatag
     1261 caaagaaaaa ttcacagaag gaaagacatt gatggagtgg ataaatgctt tgatgaacta
     1321 ttttgtactt catccatatc gcctatgggg gcttcttttt ataatagcgt tcacaaaatc
     1381 cactttactc atttcggcga tattgccacc agcatcaatt atgatggcca ctgtaattac
     1441 agtcagcaag acgactcttt caccatggga agctggaatc acggtcatga gtggcgcatg
     1501 gtgcggtact atcgttaatt accatcttgg tataataatt gggcatatcc ctcaac
//
LOCUS       DAJTYL010000270         1545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYL010000270 DAJTYL010000000
VERSION     DAJTYL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.279"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..281
                     /locus_tag="OO572_004073"
     CDS             <1..281
                     /locus_tag="OO572_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6662851.1"
                     /translation="EITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLA
                     TDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE"
     gene            307..1428
                     /gene="gmd"
                     /locus_tag="OO572_004074"
     CDS             307..1428
                     /gene="gmd"
                     /locus_tag="OO572_004074"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6662852.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1431..>1545
                     /locus_tag="OO572_004075"
     CDS             1431..>1545
                     /locus_tag="OO572_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="bifunctional GDP-4-dehydro-6-deoxy-D-mannose
                     epimerase/ GDP-4-dehydro-6-L-deoxygalactose
                     reductase;catalyzes the formation of GDP-fucose from
                     GDP-4-dehydro-6-deoxy-D-mannose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6662853.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDE"
BASE COUNT          384 a          406 c          417 g          338 t
ORIGIN      
        1 gtgaaataac cattggcgca cattcggtga tatcgcaaaa aagttattta tgcaccggta
       61 gccacgacca tgcaagtcaa catttcacca ttaacgccac gcctattgtg attggcgaga
      121 aatgctggct ggcaaccgat gtctttgttg ccccaggcgt cacaatcggc gacggcaccg
      181 tcgtgggtgc acgaagcagt gtttttaaat cgcttccggc aaatgtggtt tgccggggga
      241 atcccgcagt ggtgatacgc gaacgcgttg aaactgaata aattcaaaaa tacagaggaa
      301 taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg ttcttacctg
      361 gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg cgcatcgtca
      421 ttcaacaccg agcgcgtgga tcacatttat caggatccgc acacctgcaa cccgaaattc
      481 catctgcatt atggcgacct gagtgatacc tctaacctga cgcgcatttt gcgtgaagta
      541 cagccggatg aagtgtacaa cctaggcgca atgagccacg ttgcggtctc ttttgagtca
      601 ccagaatata ccgctgacgt cgacgcgatg ggtacgctgc gcctgctgga ggcgatccgc
      661 ttcctcggtc tggaaaagaa aactcgtttc tatcaggctt ccacctctga actgtatggt
      721 ctggtgcagg aaattccgca gaaagaaacc acgccgttct acccgcgatc tccgtatgcg
      781 gtcgccaaac tgtacgccta ctggatcacc gttaactacc gcgaatccta cggcatgtac
      841 gcttgtaacg gaattctctt caaccatgaa tccccgcgcc gcggcgaaac cttcgttacc
      901 cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg cctgtacctc
      961 ggcaatatgg attccctgcg tgactggggc cacgccaaag actacgtaaa aatgcagtgg
     1021 atgatgctgc aacaggaaca gccggaagat ttcgttatcg cgaccggcgt tcagtactcc
     1081 gtacgtcagt ttgtggaaat ggcggcagca cagctgggca tcaaactgcg ctttgaaggc
     1141 acgggtgttg aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc gccgggcgtt
     1201 aaaccgggtg atgtgattat cgccgttgat ccgcgttact tccgtccggc agaagttgaa
     1261 acgctgctcg gcgacccgac caaagcgcac gaaaaactgg gctggaaacc ggaaatcacc
     1321 ctcagagaga tggtgtctga aatggtggct aatgacctcg aagcggcgaa aaaacactct
     1381 ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc atgagtaaac
     1441 aacgcatttt tatcgctggc cattgtggga tggtcggttc tgccatcaag cggcagctcg
     1501 aacagcgcgg tgatgtggag ctggtattac gtacccgcga tgagc
//
LOCUS       DAJTYL010000271         1528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYL010000271 DAJTYL010000000
VERSION     DAJTYL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.280"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..901
                     /locus_tag="OO572_004076"
     CDS             <1..901
                     /locus_tag="OO572_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6662854.1"
                     /translation="MRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAY
                     GIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLLSGGWL
                     PLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRA
                     INVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYG
                     WRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRLRGKLYLLLQRNA
                     LRAPDQFEIPPNRVIELGTQVEI"
     gene            1068..1487
                     /gene="rbsD"
                     /locus_tag="OO572_004077"
     CDS             1068..1487
                     /gene="rbsD"
                     /locus_tag="OO572_004077"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6662855.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
BASE COUNT          369 a          373 c          385 g          401 t
ORIGIN      
        1 gatgcgcatt attcacacct ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa
       61 ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact ccagcaacct
      121 ggcggcggcg tacgggattg cggtgaccgg aaccatggta ctgacgtcta ttctctcgac
      181 taccgtggca cgtcagaact ggcactggaa taagtatttt gttgcgctga tcctgattgc
      241 tttcctttgt gtcgatattc cattgttcac cgctaacctc gataaactgc tctccggcgg
      301 ctggttgcca ttgagcctcg gtactgtgat gtttatcgtg atgaccacct ggaagagcga
      361 gcgtttccgc ttgctgcggc ggatgcatga acatggtaac tctctggaag cgatgattgc
      421 ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc
      481 aatcaacgtc attccctttg cgctgatgca taaccttaaa cataacaagg tattgcatga
      541 gcgggtgatt ctgttaactc tgcgcaccga agacgcgcca tatgtccata acgtccgtcg
      601 ggtacagatt gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt atggttggcg
      661 agaaacgcca aacgtggaag aagttttcca ccgctgcggt ctggaaggat taagttgccg
      721 gatgatggaa acctccttct ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg
      781 gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcacc
      841 agatcaattt gaaatcccgc caaacagggt tatcgaactg ggtactcagg tcgaaatcta
      901 acgccagacg cctcctttct tcataagggg gcgtttttgt tttcatggtt aatcaccatg
      961 taaaacgttt cgaggttgat cacatttccg taacgtcacg atggttttcc caactcagtc
     1021 aggattaagc tgcggatcag cgaaacgttt cgctgatgga gaaaaaaatg aaaaaaggca
     1081 ccgttcttaa ttctgatatt tcatcggtga tctcccgtct gggacatacc gatacgctgg
     1141 tggtgtgtga tgctggttta cccatcccca aaagtacaac gcgtatcgat atggcattaa
     1201 cccaaggtgt accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa atgcaggtcg
     1261 aggctgccat tatcgcggaa gagatcaaac aacataatcc gcaactccac gaaacgttgc
     1321 tcactcacct tgagcagctg caaaaacacc agggaaatac cattgaaatt cgttacacca
     1381 cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc agcggagaat
     1441 gttctccgta tgcgaatatc attctctgtg ctggcgtgac gttctgaggc cgtcatggaa
     1501 gcattacttc agcttaaagg catcgata
//
LOCUS       DAJTYL010000272         1480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYL010000272 DAJTYL010000000
VERSION     DAJTYL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.281"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            206..457
                     /locus_tag="OO572_004078"
     CDS             206..457
                     /locus_tag="OO572_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662856.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OO572_004079"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO572_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..>1480
                     /locus_tag="OO572_004080"
     CDS             804..>1480
                     /locus_tag="OO572_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6662857.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          362 a          375 c          388 g          355 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg gttttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca
     1321 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
     1381 aaaaaatccg ctcaatgtac cgtgaaagcg acatcgtgcc gggagagcag accgccacca
     1441 gcatactgaa gcagcgcaca aaaaatcttg cgccgctgcc
//
LOCUS       DAJTYL010000273         1456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYL010000273 DAJTYL010000000
VERSION     DAJTYL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.282"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..193
                     /locus_tag="OO572_004081"
     CDS             <1..193
                     /locus_tag="OO572_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6662858.1"
                     /translation="KAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQAL
                     HRRDVQVPDLLSLRGNAHA"
     gene            186..1145
                     /gene="ssuA"
                     /locus_tag="OO572_004082"
     CDS             186..1145
                     /gene="ssuA"
                     /locus_tag="OO572_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6662859.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1142..>1456
                     /locus_tag="OO572_004083"
     CDS             1142..>1456
                     /locus_tag="OO572_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6662860.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALF"
BASE COUNT          336 a          403 c          378 g          339 t
ORIGIN      
        1 gaaagctcag gagatcctgc acggcgtgtt tgccgatgac tcacaattaa ttgattacca
       61 tcacagaccc cagttcacgc caaatctgca aacccatctt gataccgcgc tagaaacttt
      121 ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa
      181 tgcccatgcg taacatcatt aaactggcgc tggcgggatt gcttagcgtc tctacttttg
      241 cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg
      301 gtatggtgct ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa tcaaaaatct
      361 cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg
      421 atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg gctgatttgg
      481 tatatgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg gcagaaaaca
      541 gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag aaaggttcca
      601 gttcacacaa ccttttactg cgtgcactgc gtcaggccgg acttaaattc accgatatcc
      661 agcccactta cctgacgcct gccgatgccc gcgccgcgtt ccagcaaggt aacgttgacg
      721 cctgggctat ctgggatccc tactactccg ctgcgttatt acagggcggc gtgcgggtgc
      781 tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctggcagct cgtccctatg
      841 cagaaaaaaa cggcgctttt attcagggcg tactggcaac ctttagtgag gccgatgcgt
      901 taacccgcag ccagcgcgaa caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
      961 caccggtgat tgcctcttac ttagatcatc gccctcctac caccatcaaa ccggttaacg
     1021 ccgaggttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaac cgtctggtgc
     1081 cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa ggaaaacaat
     1141 tatgagtctg aatatgttct ggtttttacc gacccacggt gacgggcact atctgggaac
     1201 ggaagaaggt tcacgcccgg ttgatcacgg ttatctgcaa caaattgcgc aagcggcgga
     1261 tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag atgcgtggct
     1321 ggttgcagcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgtcc
     1381 cagcgtaacc tcacctactg ttgccgcccg ccaggccgcc acgcttgacc gtctctcaaa
     1441 tggtcgtgcg ttgttt
//
LOCUS       DAJTYL010000274         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYL010000274 DAJTYL010000000
VERSION     DAJTYL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.283"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            82..1275
                     /locus_tag="OO572_004084"
     CDS             82..1275
                     /locus_tag="OO572_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6662861.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OO572_004085"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OO572_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          314 c          339 g          368 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac cattccatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJTYL010000275         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYL010000275 DAJTYL010000000
VERSION     DAJTYL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.284"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..194
                     /locus_tag="OO572_004086"
     CDS             <1..194
                     /locus_tag="OO572_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="regulator for the transport and utilization of the
                     aromatic beta-glucosides arbutin and silicin; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bgl operon transcriptional regulator BglJ"
                     /protein_id="HCR6662862.1"
                     /translation="FMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEA
                     EDILLCMRHCEASNVLHPY"
     gene            complement(232..1020)
                     /gene="fhuF"
                     /locus_tag="OO572_004087"
     CDS             complement(232..1020)
                     /gene="fhuF"
                     /locus_tag="OO572_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6662863.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1161..1397
                     /locus_tag="OO572_004088"
     CDS             1161..1397
                     /locus_tag="OO572_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6662864.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          359 a          318 c          380 g          376 t
ORIGIN      
        1 gctttatgtc gcgtggctac tcaatgacac aaattgccga gcagcttaaa cgtaatatca
       61 aaacgatccg agcgcataaa tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag
      121 ggttgttgga ggccgaagat attctgttat gtatgcggca ttgcgaagca agtaatgtgt
      181 tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct ttcatttcag
      241 cgtacaatag ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg
      301 caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt caccgttcgt
      361 gagcgttttc tcaaaaaaga gggcatggcg cagcgattca acagtagcct cgccgagcag
      421 ttgcttcatc tcagtgagat accagttgat caaataaccg gtattactcc agataagttt
      481 gccgttgatc tctccggtcg cttctagtgc ttgcacaacc ggaaccagcg cctggctgat
      541 taacgtttcc attcgctgct gcggcgaatg tggtgttgcg tttttatctt cacacacatc
      601 gacccagaaa caggcgacgc gtccggtttc gtgaaactca gcatggaaat gttccggcga
      661 cacatctaat gctttttcct gcgtcagtag cgccagcatt aatggtggca ccatcaggcc
      721 gatataccat tgtgcccata gtgagatcag cggtttgttc tcgcggatca tcgttggttg
      781 gttgcgatag atatgatcgg aatagaccgc cagtagagaa cttagcgcat tcggtgatga
      841 ccattgcgcc agcgtcatgg cgttaagcgg agcgggttca tccaggcgga taaactccag
      901 caaatgttca cgatgtttgg cgatcatcgc ccgtacggct tgtgcaagcc cagcatcctg
      961 cggctggaga tgcgttcgcc agatgacatc ttcatagagc ggtgcggaac gataggccat
     1021 aatcgtgata gtaatctaaa tgataatgat tgctaatcct agcgataggt ttactcgata
     1081 gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg gttttcatta
     1141 tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc
     1201 tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat cagtggcgga
     1261 ttgtcattct gatgggatta gtattgacgc cggtgatgct gtatcacaaa cagttgcggc
     1321 attacatttt gctaccatcg tgcctggcgc ttattgctgg catcatgctg atgataatga
     1381 atttgaatca gggatgacaa aacagggaag aaacaagaaa ggaagtaaag ata
//
LOCUS       DAJTYL010000276         1413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYL010000276 DAJTYL010000000
VERSION     DAJTYL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.285"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(68..733)
                     /locus_tag="OO572_004089"
     CDS             complement(68..733)
                     /locus_tag="OO572_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6662865.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(902..1258)
                     /locus_tag="OO572_004090"
     CDS             complement(902..1258)
                     /locus_tag="OO572_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662866.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1316..>1413)
                     /locus_tag="OO572_004091"
     CDS             complement(1316..>1413)
                     /locus_tag="OO572_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6662867.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          348 a          364 c          298 g          403 t
ORIGIN      
        1 tcaagcaccc gcagtcattc tacttacctc gccaatatga aatcaatcag aaaggtgccc
       61 cataaaatca ctccttctct tctttaccgt agtggagttg accaattttg ataagagggc
      121 gtccctgaga tttgcggtgt agattggtat cgcgcagaga atacacacag ccacaatatt
      181 cctgctgata gaatttttcg cgcttgctga tttcaatcat acgggacgag ccgccctgct
      241 tgcgccagtt ataatcccag tacaccatgc ccggataatg cgcgacggct cgccgcccac
      301 agtcgttaac ctgctgcata tttttccagc gtgaaatgcc cagtgaactg ctgatcacac
      361 tgaaaccatt ttcagcggcg tacagcgctg tccgctcaaa acgcatgtca aaacacatgg
      421 tgcaacggat ccctcgttcg ggctcccatt ccattccttt ggctcgttca aaccagttgt
      481 ctgtgtcgta atcagcatca ataaacggca cgccgtgttg ttcagcaaag cgaatattct
      541 catccttacg aattaaatac tctttctgag gatgaatgtt cgggttgtag aaaaagatgg
      601 tgtagtcgat ttccgaggcc tgaagcgcct ccatcacctc accggaacat ggagcacagc
      661 aagagtgcag tagtagtttg tttgccccgt ttgggagctc caatttgggc cgtttgaaat
      721 cagcaactgt cataaatatg tttattgagg tcataaaaat atcaaagagt gtagcattag
      781 aacggggcta tcggaaacag atgtgtgact gctccccgcc ctttcgggcg gtctcctgat
      841 gatttgaggg tgcagaaatc cctccggtta aggattaaat ttttaacagt gctaaattta
      901 attattcagt tctggatttt gtcgccctgc gtatccgcgc tttcgcgtta cgctcaatct
      961 gtatcagctt ttctatattt ctccgccttt cctgttcctc ctggcgcaat agccttacat
     1021 catctgccag tctggtttct ctttttgcca cagagagcat ccagtcaaac ggctccacaa
     1081 ccgcaccgca gattttacag cggacctgac gctctttttc gtcaacccgt acagaggcgt
     1141 gatggcaata tggtctttcc gatggcttat aaagaaaatt aacctgatta cgagggtcat
     1201 cctcttttat cggaaataaa acgatattgc ttaactcatc ttctggtttt atttccatgc
     1261 tcctctcctt tgatgcgaat gccagcggta attgaagcct gatagctaat ttcattcaca
     1321 gtatcgcctc ctgaaaatta ccctgataga aagccagtac acgctgcata gcttcgctct
     1381 tccggcactc gcgacagatt atattcaggc gcc
//
LOCUS       DAJTYL010000277         1380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYL010000277 DAJTYL010000000
VERSION     DAJTYL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.286"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO572_004092"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO572_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6662868.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO572_004093"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO572_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..>1380)
                     /locus_tag="OO572_004094"
     CDS             complement(1294..>1380)
                     /locus_tag="OO572_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6662869.1"
                     /translation="LARKTICFSRSVEIHEKVIGSFIEKHIF"
BASE COUNT          440 a          208 c          290 g          442 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
//
LOCUS       DAJTYL010000278         1329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYL010000278 DAJTYL010000000
VERSION     DAJTYL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.287"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..360
                     /locus_tag="OO572_004095"
     CDS             <1..360
                     /locus_tag="OO572_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6662870.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO572_004096"
     CDS             361..726
                     /locus_tag="OO572_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6662871.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..>1329
                     /locus_tag="OO572_004097"
     CDS             723..>1329
                     /locus_tag="OO572_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6662872.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLD"
BASE COUNT          369 a          280 c          374 g          306 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggata
//
LOCUS       DAJTYL010000279         1322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYL010000279 DAJTYL010000000
VERSION     DAJTYL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.288"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            83..449
                     /locus_tag="OO572_004098"
                     /pseudo
     CDS             83..449
                     /locus_tag="OO572_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            377..850
                     /locus_tag="OO572_004099"
                     /pseudo
     CDS             377..850
                     /locus_tag="OO572_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          323 a          334 c          399 g          266 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtccgc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcgccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatacacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg
      901 cagcacaaga gatgagggaa ggccccttgg tgtcggcgtg caggcgcagg caccaaacca
      961 tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg
     1021 agccgatcag gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa
     1081 gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg
     1141 cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac
     1201 ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca
     1261 atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa
     1321 tt
//
LOCUS       DAJTYL010000280         1307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYL010000280 DAJTYL010000000
VERSION     DAJTYL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.289"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..68
                     /locus_tag="OO572_004100"
     CDS             <1..68
                     /locus_tag="OO572_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6662873.1"
                     /translation="TYLTNLYFKIREDKRKAARGE"
     gene            72..701
                     /locus_tag="OO572_004101"
     CDS             72..701
                     /locus_tag="OO572_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662874.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            767..1300
                     /locus_tag="OO572_004102"
     CDS             767..1300
                     /locus_tag="OO572_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6662875.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
BASE COUNT          363 a          269 c          357 g          318 t
ORIGIN      
        1 tgacttatct gacgaatctg tatttcaaaa taagagaaga caagcgtaag gctgcacggg
       61 gagagtaaat aatgaagcat gaagaaatga atcagcgatt caatcacctg gaaaatgaaa
      121 tcactgagct taataaaaaa ttgtcggcgt tggtgagttc tgaagatgaa aacaaacgcc
      181 gcgatgagca ttatgcggcg atttacgatt actgccacaa agtcgctcac gagactttta
      241 tgaagttttt gcaggaaaag tttttaccgg ccgcattgtc agaaaaagag gcggcttacc
      301 tgcggcctga gtacgtcatt acggttaaca gtgccggaga ggaagagcat aaaagtgatt
      361 ttattgcgtc tgcaccggat aaagaccagg aaccccatcg gcctttcagg gtttcctgtg
      421 aagagggtga gttcgtcgtt tatgagaacg gaaaacctgt ccgggcatcg catcatcact
      481 gcctgaaaat tattaatctc gctatcagat gcctgaaaga cgaaaacacc agagttatga
      541 aacgcattgg ccgttgcatg gggtatttgc aggtggcggc agagattgaa gcacttgcca
      601 gtggtgcaga catggatgct gcggtgcggg aggctcttct tcgtgatttc aatactcccc
      661 ctttaagaaa gagcctgatg actggatcca gccggggctg acttatctta aaaggcgtat
      721 ataagttggc tcgttatttg ttgccgataa atcctgataa atatccatga gcgcaaaaat
      781 caaatacggc ctgtcggctg ctgttctggc gctgattgcc gcaggcgcgt ctgctcccca
      841 gatacttgac cagtttctga acgaaaaaga gggtaatcac acaacggcat accgtgatgg
      901 ttctggcata tggaccatct gtcgtggtgc cacaatggtg gatggtaagc ctgtcatacc
      961 gggaatgaag ctgtcgaagg aaaaatgtga ccaggttaac gctattgaac gtgataaggc
     1021 gctggcatgg gtggagcgca atattaaagt acctctgacc gaaccccaga aagcgggtat
     1081 agcgtcattt tgcccctata acattggccc cggtaagtgt ttcccgtcga cgttttataa
     1141 gcggctgaat gccggtgatc gtaagggcgc atgcgaggcg attcgctggt ggataaaaga
     1201 tgttgggcgc gattgccgca tacgttcaaa taactgctat ggacaggtta ttcgtcgtga
     1261 ccaggaaagc gcattagcct gttgggggat agatcaatga gcagagt
//
LOCUS       DAJTYL010000281         1304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYL010000281 DAJTYL010000000
VERSION     DAJTYL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.290"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            538..822
                     /locus_tag="OO572_004103"
     CDS             538..822
                     /locus_tag="OO572_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6662876.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            822..1097
                     /locus_tag="OO572_004104"
     CDS             822..1097
                     /locus_tag="OO572_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6662877.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          342 a          328 c          329 g          305 t
ORIGIN      
        1 gtgcctgaca gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca
       61 ttaagtatca cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata
      121 aaatctcgta gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact
      181 ggtttgtact ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg
      241 gccctcacgg cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac
      301 cactccgacc gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc
      361 ggagggggac tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt
      421 acacctgtgc tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg
      481 ccttgttccg tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg
      541 caaatgaaaa acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct
      601 atggctgata aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc
      661 aaacaggcgc tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa
      721 gccctggacg atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg
      781 gacagcctca gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca
      841 gtaaggcact ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg
      901 ctgccgccag aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac
      961 gtatgggaga acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg
     1021 agtacgaaat ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca
     1081 cacgagagaa caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat
     1141 tctggagcat tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat
     1201 aaaataaatt acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct
     1261 ccgggctggc aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTYL010000282         1290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYL010000282 DAJTYL010000000
VERSION     DAJTYL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.291"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..289
                     /locus_tag="OO572_004105"
     CDS             <1..289
                     /locus_tag="OO572_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662878.1"
                     /translation="LKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYY
                     AGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            291..896
                     /locus_tag="OO572_004106"
     CDS             291..896
                     /locus_tag="OO572_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6662879.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            883..>1290
                     /locus_tag="OO572_004107"
     CDS             883..>1290
                     /locus_tag="OO572_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662880.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLED"
BASE COUNT          300 a          306 c          359 g          325 t
ORIGIN      
        1 gctgaaaaaa agtgactacc tgccttcaga tattacccga caggtacagc aatccgtgct
       61 ggacgcattt aatggtacag atggtggtct gcgggcaaga gttgcctctg ttgtctctgc
      121 cgggcgctac tatgccggca tttacaaaac cgatccggaa catattgata ttctgggcct
      181 tactgtgagc cgtgatggtt cgtcatggac aacggctgtc actttcggga tagatgagat
      241 tccggttctg gatgtgtcgg atatcagtgt gaaactccag gaggcataac gtgcagaatg
      301 tggctgccac tgtgcttgcg cagtatgctg ccagcccccg actcaatgcc ctcattaaca
      361 gctttaacgc agcgctttcc cccgacagtt ttatcagtga tttttatggt cttatctgga
      421 acatcgatac cgcagaaaag tatggtcttg atgtctgggg aaagattgtg ggtgtcagtc
      481 gctggctgac ggtaaaggac gattttaatt acctgggctt cagcgagtcc aggatggaca
      541 ccccggtaat ggatgatccc tgtccgttta atcaggcacc gttttacaac ggaaaatcgg
      601 atacccggac tgttgacctg tctgatgctg tataccggcg gctgatactg atgaaagcca
      661 tgtcgaacat tactgactgt tccgttccgg atattaacct gatgctgaga tttatgttcg
      721 gaaaaaaacg ccgggcttat gttctgaata atggcgggct gaggatgagt tacgtctttg
      781 agtccgcgct ctcgttggca gaactggcga ttatccagtc gtcgggtgca ctgccatccc
      841 cgccgggtgt ttatgtttca gtagttttaa aggagtcccg taatgaaggc cagtgataaa
      901 ccccgccagc tggcggtccc ctttgcgagt accggagata aaaaccgtat cccggacaag
      961 gcgacacagc agaccagaga gagcggtaat gctgcgtatg attcaggttt tcctccggtg
     1021 accatgacag cggtctcagc gggaggtata ccgccacacg gcaaggattt taacggtctg
     1081 atgtacgata ttaccgcagc aatacggttc gcccaggctg gcggtttgta cacgtataat
     1141 gccggttttg cgggggccat tggtggatat gcaaaaggag ccattctcgc cggagtcgca
     1201 acaacagcgg tctggctgaa taccacggac gataacctga ccgatcctga aggctccgac
     1261 agtgcgggct gggtaaatct tcttgaggat
//
LOCUS       DAJTYL010000283         1285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYL010000283 DAJTYL010000000
VERSION     DAJTYL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.292"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>1285)
                     /locus_tag="OO572_004108"
     CDS             complement(<1..>1285)
                     /locus_tag="OO572_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6662881.1"
                     /translation="REALWSKWDKASDSQRSLAEKWLPSIQATDEMLNQGISTKTAFA
                     TVAGHYQVSASTLRDKYYQVQKFAKPDWAAALVDGRGASRRNVHKSEFDEDAWQFLIA
                     DYLRPEKPAFRKCYERLELAAREHGWSIPSRATAFRRIQQLDEAMVVACREGEHALMH
                     LIPAQQRTVEHLDAMQWINGDGYLHNVFVRWFNGDVIRPKTWFWQDVKTRKILGWRCD
                     VSENIDSIRLSFMDVVTRYSIPEDFHITIDNTRGAANKWLTGGAPNRYRFKVKEDDPK
                     GLFLLMGAKMHWTSVVAGKGWGQAKPVERAFGVGGLEEYVDKHPALAGAYTGPNPQAK
                     PDNYGDHAVDAELFLKTFAEGVAMFNARTGRETEMCGGKLSFDDVFEREYARTIVRKP
                     TEEQKRMLLLPAEAVNVSRKGEFTLKVGGSLKGAK"
BASE COUNT          288 a          371 c          300 g          326 t
ORIGIN      
        1 tttttcgcgc ctttaaggga gccgccaact ttaagcgtaa actcgccttt gcgtgaaacg
       61 ttcaccgcct cggcaggcag taacagcatc cgtttttgtt cttcggttgg cttacgcaca
      121 atcgttctgg cgtattcacg ctcgaaaaca tcatcaaacg agagtttgcc cccgcacatt
      181 tctgtttcac ggcctgttct ggcattgaac atcgccacac cttcggcaaa ggttttcaga
      241 aacagctctg catcaacagc gtggtcgcca tagttatcag gttttgcctg cggatttggc
      301 cccgtatatg cgccagccag tgccggatgc ttatcaacgt attcctcaag cccaccaaca
      361 ccgaaagcac gttcaacagg ttttgcctgg ccccagcctt taccggcaac aacgcttgtc
      421 cagtgcattt tcgcacccat cagtaaaaac agtccttttg gatcgtcctc ttttacctta
      481 aagcggtagc gattgggcgc gcctcccgtc agccatttat tcgcagcacc acgggtgtta
      541 tcaatggtga tgtgaaaatc ctccgggata ctgtagcgag tcacaacatc catgaacgag
      601 aggcgaattg aatcaatgtt ctcgctcaca tcgcagcgcc agcccagaat ttttcgggtt
      661 ttcacatcct gccagaacca tgttttcgga cggatcacat caccgttaaa ccagcgtaca
      721 aagacgttat gcagataacc gtcgccgttg atccactgca tggcgtccag gtgttccaca
      781 gttcgctgct gtgccggtat cagatgcatc agtgcatgtt caccttcacg acaggcaaca
      841 accattgcct cgtccagttg ctgaatccgg cgaaaggccg tggcacggga gggaatactc
      901 cagccatgct cgcgggctgc cagttccaga cgctcataac atttgcggaa ggcgggtttt
      961 tccggtcgca gataatctgc aatcagaaac tgccaggcat cctcgtcaaa ttcacttttg
     1021 tgaacattgc gacgggatgc tccacgtcca tcaacaagtg cagccgccca gtcaggcttc
     1081 gcaaacttct gtacctggta atacttgtcc cgcaaagtgg atgcgctgac ctggtaatgc
     1141 cctgcaacgg tcgcaaaagc cgttttcgtt gaaatcccct ggttcagcat ttcgtctgtg
     1201 gcctgaattg agggcaacca tttttcagcc aggctgcgct gggaatcgct ggctttatcc
     1261 catttgctcc atagtgcctc acgat
//
LOCUS       DAJTYL010000284         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYL010000284 DAJTYL010000000
VERSION     DAJTYL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.293"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            34..435
                     /locus_tag="OO572_004109"
     CDS             34..435
                     /locus_tag="OO572_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6662882.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO572_004110"
     CDS             447..818
                     /locus_tag="OO572_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662883.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO572_004111"
     CDS             805..>1275
                     /locus_tag="OO572_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6662884.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTYL010000285         1267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYL010000285 DAJTYL010000000
VERSION     DAJTYL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.294"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..935
                     /locus_tag="OO572_004112"
     CDS             <1..935
                     /locus_tag="OO572_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6662885.1"
                     /translation="VKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQDNLATA
                     MRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLNDIRGRY
                     ECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEMMREYA
                     NKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAENQQAQ
                     IQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDARANAEL
                     LLKDADQTHSQRMDFANLMRQVQTPSGGVAETPQ"
     gene            949..>1267
                     /locus_tag="OO572_004113"
     CDS             949..>1267
                     /locus_tag="OO572_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6662886.1"
                     /translation="MNPTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDY"
BASE COUNT          373 a          319 c          332 g          243 t
ORIGIN      
        1 cagtgaaaga ggtggctagt cttggcgttg atgcgcaggc ggcaaatggt caggtcgctt
       61 tcgataccgt caatcaactg aacatgcggg cagaccttga gacatacgtg tttcaggata
      121 acctggctac cgcaatgcga cgtgatggcg agatttatgc ctcaatggtc aacgatattt
      181 atgacgttcc tcgccatgta acgctgactc ttgaagacgg aagcgagaaa gacgttcaac
      241 tctatgcgca agttgtcgat taccagtccg gcaatgtggt cacactcaac gacattcgcg
      301 gtcgctatga gtgctataca gacgttggac catccttcca gagcatgaag gaacagaatc
      361 gcgcagagat tcaggagttg ctaacaaagg ttccacaagg tactccagag ttccagatgc
      421 tgatgctgca atacttcacg ctgcttgacg gtaaaggcgt cgagatgatg cgagagtacg
      481 cgaacaagca actggtgatg atggggctga agaaaccaga aacacctgaa gagatggaga
      541 tggtacagca ggctcaacag cagccgcagc agccatcagc agagcaaatt caggcgcagg
      601 gtatccttct gcaaggtcag gctgaattgc tcaaggcaga gaaccaacag gcgcagattc
      661 aggttgaagc cgccaaggtt gaagctcaaa accaactcaa cgccgcgaag attgcagaaa
      721 tcttcaacaa tatggacctc gacaagcagg cagaactgcg tgagtacctc aagctcgtag
      781 gtcaattcca gcaacagcgc agcaaagacg ctcgcgctaa cgctgagctg cttcttaagg
      841 atgcagacca gactcattca caacgcatgg atttcgcgaa tcttatgcgt caagttcaaa
      901 ccccctccgg cggagtagcc gagacacctc aataagagag agttaatcat gaacccaacc
      961 accgacattc aggcttctga agaattaacc ctgcccggca atcatgcagc ggcatctgct
     1021 gatggcttag ttgtcgataa tgccaacgac aacgcaggtc aggaagaagg cttcgagatt
     1081 gtcctgaaag acgatgagaa accaaaacaa gacccggcaa ctaatgctga atttgcccgc
     1141 cgccgcatcg aacgcaaacg ccagcgtgag cttgagcaac agatggaagc ggttaagcgt
     1201 ggagagttgc cggagcacct gcgggtgaac cctgagttac caaaacaacc agaccctaac
     1261 gattatc
//
LOCUS       DAJTYL010000286         1241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYL010000286 DAJTYL010000000
VERSION     DAJTYL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.295"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..789
                     /gene="ccp"
                     /locus_tag="OO572_004114"
     CDS             <1..789
                     /gene="ccp"
                     /locus_tag="OO572_004114"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6662887.1"
                     /translation="TISCAHCHTLNAGGVDGRKTSIGVGGAVGPINAPTVFNSVFNVE
                     QFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQFLEVYPQGFSGEN
                     ITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNKCATCHGGIILGGR
                     SFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVALTAPYFHRGDVPT
                     LDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1000..>1241
                     /locus_tag="OO572_004115"
     CDS             1000..>1241
                     /locus_tag="OO572_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6662888.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          344 a          237 c          310 g          350 t
ORIGIN      
        1 accatttcat gcgctcattg ccatacgttg aatgcggggg gcgtcgatgg cagaaaaaca
       61 tcgattggtg ttggtggcgc agttgggccg attaatgcgc cgacggtatt taactcagta
      121 tttaacgttg agcagttctg ggatggtcgt gcggcaacat tgcaggatca ggcaggtgga
      181 ccgccgttga acccgattga aatggcgtcg aaatcctggg acgaaattat tgctaagctg
      241 gaaaaagatc cgcagcttaa agcgcagttc ctcgaagttt atccgcaagg tttcagtggc
      301 gaaaatatta ctgatgccat tgctgaattt gagaaaacat taattacgcc ggattcccca
      361 tttgataaat ggttgcgtgg agatgaaaat gcgctgacgg cgcaacagaa aaaaggctat
      421 caattattta aagataataa atgtgcaact tgtcatggtg gtattattct cggcggacgt
      481 tcctttgaac cgttggggct gaaaaaagac tttaactttg gggaaattac ggcggcggat
      541 attggtcgta tgaatgtgac taaagaagag cgtgataaat tgcgtcagaa agtacccggt
      601 ttacgtaacg ttgctttaac ggcaccgtac ttccatcgcg gtgacgtgcc gacgctggac
      661 ggggcggtga aactgatgct gcgctatcag gtaggcaaag agctgccgca ggaggatgtg
      721 gatgatatcg tagctttcct gcacagtctg aacggggtgt acacgccgta tatgcaggat
      781 aaacaataat taatttgatc gcccgaacag gaatgtttgg gcgattttta ttacgataat
      841 aaagtctgtt tttaatatta tcatgttaaa tgtttatatt ataaaaagtc gtttttctgc
      901 ttaggatttt gttatttaaa ttaagcctgt aatgccttgc ttccattgcg gataaatcct
      961 acttttttat tgccttcaaa taaatttaag gagttcgaaa tggaccagaa gctgttaacg
     1021 gatttccgct cagaactact cgattcacgt tttggcgcaa aggccatttc tactatcgcg
     1081 gagtcaaaac gatttccgct gcacgaaatg cgcgatgatg tcgcattcca gattatcaat
     1141 gatgaattat atcttgatgg caacgctcgt cagaacctag ccactttctg ccagacctgg
     1201 gacgacgaaa acgtccataa attgatggat ttgtcgatca a
//
LOCUS       DAJTYL010000287         1236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYL010000287 DAJTYL010000000
VERSION     DAJTYL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.296"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..297
                     /gene="curA"
                     /locus_tag="OO572_004116"
     CDS             <1..297
                     /gene="curA"
                     /locus_tag="OO572_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6662889.1"
                     /translation="GLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYGHR
                     IHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            495..1160
                     /gene="mcbR"
                     /locus_tag="OO572_004117"
     CDS             495..1160
                     /gene="mcbR"
                     /locus_tag="OO572_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6662890.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          382 a          257 c          289 g          308 t
ORIGIN      
        1 ggcttagtga gcagctataa cgctacagag ctaccacccg gtccggatcg tttacccctg
       61 ttgatggcaa cggtgctgaa aaaacgtatt cgcttgcaag ggtttattat cgctcaggat
      121 tatggtcacc gcatccatga gtttcagaag gagatggggc aatgggtgaa agaggataaa
      181 atccactacc gcgaagatat tactgatggt ttagagaatg cgccacagac gtttatcggc
      241 ctgctgaagg gtaaaaactt cggcaaagtg gtgatccgcg tcgcgggtga tgattaaagt
      301 ttcaacggcg gcgtaagccg ccataaaaat ccaggcaaaa agcttatttt cagctttaat
      361 taactaacca cgtcattgtt ctgctgctaa taacaacaaa ggtgggtggt ttatgattat
      421 tcactttaat acactaggtg aactccttct gccatgcagg cagggttgga cagaaaacac
      481 gcgaaggaat taaaatgcct ggaacgggaa aaatgaaaca tgtcagtttg actctgcagg
      541 ttgagaacga cctgaaacat cagcttagta ttggcgcatt aaaacctggc gcacgcctga
      601 ttactaaaaa tctggcggag caattaggta tgagtattac acctgtgcgt gaagcattat
      661 tacgtctggt ttcggtgaat gcgctttctg tcgcacctgc acaagcattt acagtaccgg
      721 aagtgaggca aagtcagtta gacgaaatca atcggatccg ctacgaactg gaattaatgg
      781 cagttgccct ggctgttgaa aacctctcct cgcaagacat agcagaactt caggaattgc
      841 tcgggaaatt acagcaggcg caagaaaagg gcgacatgga acaaatcatt aatgtgaaca
      901 ggctatttcg cttagcgatt tatcatcgct caaatatgcc catcctgtgt gagatgattg
      961 agcaactgtg gatcaggatg ggacctgctt tacattatct ttatgaagcg attaacccag
     1021 cggaattacg ggagcgcata gaaaactatc atctattact cgctgcgtta aaagcaaaag
     1081 acaaagaggg atgcaaacat tgtcttgctg aaattatgca acaaaatatt gctattttat
     1141 accagcaata caatcgttaa attaaatgcc cggcaaatac cgggcatcaa gtaattactc
     1201 aaatcgccac gcaatattca tgccgacacc ataatt
//
LOCUS       DAJTYL010000288         1200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYL010000288 DAJTYL010000000
VERSION     DAJTYL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.297"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..868
                     /locus_tag="OO572_004118"
     CDS             <1..868
                     /locus_tag="OO572_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6662891.1"
                     /translation="VNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHV
                     TLTLEDGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQ
                     ELLTKVPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQ
                     QAQQQPQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEI
                     FNNMDLDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQV
                     QIPSGGVAETPQ"
     gene            882..>1200
                     /locus_tag="OO572_004119"
     CDS             882..>1200
                     /locus_tag="OO572_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6662892.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDY"
BASE COUNT          357 a          298 c          314 g          231 t
ORIGIN      
        1 cgtcaatcaa ctgaacatgc gggcagacct tgagacatac gtgtttcagg ataacctggc
       61 taccgcaatg cgacgtgatg gcgagattta tgcctcaatg gtcaacgata tttatgacgt
      121 tcctcgtcat gtaacgctga cacttgaaga tggaagcgag aaagacgttc aactctatgc
      181 gcaagttgtc gattaccagt ccggcaatgt ggtcacactc aacgacattc gcggtcgata
      241 tgagtgctat acagacgttg ggccatcctt ccagagtatg aaggaacaga accgcgcaga
      301 gattcaggag ttgctcacca aggttccgca aggtactcca gagttccaga tgctgatgct
      361 gcaatacttc acgctgcttg acggtaaagg cgtcgagatg atgcgagagt acgcgaacaa
      421 gcaactggtg atgatggggc tgaagaaacc agaaacacct gaagagatgg agatggtgca
      481 gcaggcacaa caacagccgc agcagccatc agcagagcaa attcaggcgc agggtatcct
      541 tctgcaaggt caggctgaat tgctcaaggc agagaaccaa caggcgcaga ttcaggttga
      601 agctgccaag gttgaagccc aaaaccaact caacgccgcg aagattgcag aaatattcaa
      661 caatatggac ctcgacaagc aggcagaact gcgtgagtac ctcaggctcg taggtcaatt
      721 ccagcaacag cgcagcaaag atgctcgtgc taacgctgag ctgcttctta aagatgcaga
      781 ccagactcat tcacaacgca tggatttcgc gaatcttatg cgtcaagttc aaatcccctc
      841 cggcggagta gccgagacac ctcaataaga gagagttaat catggaccaa accaccgaca
      901 ttcaggcttc tgaagaatta accctgcccg gcaatcatgc agcggcatct gctgatggct
      961 tagttgtcga taatgccaac gacagcgcag gtcaggaaga aggctttgag attgtccgga
     1021 aagacgatga gaaaccaaaa caagacccgg caactaatgc tgaatttgcc cgtcgccgca
     1081 tcgaacgcaa acgccagcgt gagcttgagc agcagatgga agcggttaag cgtggagagt
     1141 tgccggagca cctgcgggtg aaccctgagt taccaaaaca accagaccct aacgattatc
//
LOCUS       DAJTYL010000289         1192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYL010000289 DAJTYL010000000
VERSION     DAJTYL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.298"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>1192
                     /locus_tag="OO572_004120"
     CDS             <1..>1192
                     /locus_tag="OO572_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6662893.1"
                     /translation="GLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNA
                     ATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATV
                     TPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAE
                     NHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQS
                     GELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEEL
                     DDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQIT
                     VQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGR
                     NLSLT"
BASE COUNT          345 a          277 c          293 g          277 t
ORIGIN      
        1 gggcctgcgt tttggtcgcg atgttaatgc ctataccagc tatgacgaaa cggtgtatca
       61 ggtgagtttg ccgactacgc agaaacaaaa tctgcaacaa gtgatggcaa tcttcagtga
      121 atggagtaat gccgcaacct ttgaaaaact cgaagtagac gctgaacgtg gcgtaattac
      181 tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc gccgcccttt
      241 cctgctggct aatacccgta atttagaccg tgaacctatc ggcctgatgg atactgtcgc
      301 cacggtcacg ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac caaataatat
      361 gacctttatc gtggtcggcg atatcgacag taaagaagcg ctggcgctga taaaggataa
      421 tttaagtaag cttccggcta acaaagcagc tgaaaatcgc gtctggccga caaaagccga
      481 aaaccacctg cgctttaata tcattaatga taaagaaaac cgggtgaacg gcatcgcact
      541 ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa agctttgtcg aacaagctga
      601 atggagcatg ttagttcagc tgttcaatca acgtctgcag gaacgcatac agtcgggcga
      661 gttgaagact atttctggcg gcactgcgcg cagcgttaaa attgcacccg attatcagtc
      721 gctgtttttc cgtgtaaatg cacgagacga taatatgcag gatgctgcga atgcattaat
      781 ggcagagttg gcaaccattg atcagcatgg tttttctgct gaagaactcg atgatgtcaa
      841 atctacccgc ctcacctggc tgaaaaatgc ggttgatcag caagctgaac gtgatttacg
      901 tatgctgacc agtcgcctgg catccagctc attaaataat acgccgttct tgtcgccgga
      961 agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa gtctggcgga
     1021 aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg taaacaatga
     1081 gcttgccgcc aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa aagagtacgc
     1141 caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa ca
//
LOCUS       DAJTYL010000290         1191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYL010000290 DAJTYL010000000
VERSION     DAJTYL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.299"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..264)
                     /locus_tag="OO572_004121"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO572_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO572_004122"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO572_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6662894.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          353 a          291 c          183 g          364 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag g
//
LOCUS       DAJTYL010000291         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYL010000291 DAJTYL010000000
VERSION     DAJTYL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.300"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(113..643)
                     /gene="cybB"
                     /locus_tag="OO572_004123"
     CDS             complement(113..643)
                     /gene="cybB"
                     /locus_tag="OO572_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6662895.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            832..>1190
                     /locus_tag="OO572_004124"
     CDS             832..>1190
                     /locus_tag="OO572_004124"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6662896.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLIS"
BASE COUNT          338 a          271 c          258 g          323 t
ORIGIN      
        1 tatggtttta tttgtcatac aaataaatat aataggcgct tccgatgtag acccgtattc
       61 ttcgcctgta ccacgggtcg gttttagtac aggcgttttc tttaaatatc cttcaggaac
      121 gtttacgcgg catcatgcgt agaagtgtgt tgtccttcca gaaatagtgg tgtgccagtg
      181 cagccgcagc gtgcaacccg atgacaaaat atcccagatt cgccagcgtc tcatgccacg
      241 actttaagct atcaacccgt tcgaaattgg cctctgaagc gtaaggcatc gtcaaaccaa
      301 acgcaaacca cgggttgccc cggttataca tcatcaccaa accaatcact ggcagcgcaa
      361 taaacaggag ataaatcacc aaatgtccca aatgcgccag tcccgtcatc atcggtttcg
      421 gcttaggtat aatcggcggg gttgggtatt tcagtcttaa caaaagacga accaccatca
      481 gcacgagaat tgagatgcca caggaaacat gaatcatgtt gatgagcggc cgatcgctac
      541 gtgggaagaa accacgaaac tccattgcgc aatacgctgc gataaccagc aaaaagacca
      601 gccagtgaat gctgatttgt aaccttgagt atttattttc cataacattt cctgctttaa
      661 cataattttc cgttaacata acgggctttt ctcaaaattt cattaaatat tgttcacccg
      721 ttttcagtac gttacgtttc actgtaagaa tggagattgc ccatcccaca atcctggtct
      781 aagcctggaa aggatcaatt ttcatccgaa cgttcctgac aggagaaaac catgagtaaa
      841 gttggtatta acggttttgg tcgtatcggt cgactggtgt tgcgtcgttt acttgaagtc
      901 aaaagcaaca tagacgttgt cgctattaat gatcttactt ccccaaaaat tctcgcctat
      961 ctgctgaaac atgattcaaa ctatggtccg ttcccctgga gcgttgattt tacggaagat
     1021 tcacttatcg ttgatgggaa aagtatcgcg gtttacgccg aaaaagaggc taaaaatatt
     1081 ccgtggaaag cgaaaggggc agaaatcatt gtcgaatgta ctggctttta tacctccgcc
     1141 gataaatcgc aggcgcatct tgatgctggt gcgaagaagg tgttgatttc
//
LOCUS       DAJTYL010000292         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYL010000292 DAJTYL010000000
VERSION     DAJTYL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.301"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..651)
                     /gene="deoR"
                     /locus_tag="OO572_004125"
     CDS             complement(<1..651)
                     /gene="deoR"
                     /locus_tag="OO572_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6662897.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRP"
     gene            complement(709..>1151)
                     /gene="ybjG"
                     /locus_tag="OO572_004126"
     CDS             complement(709..>1151)
                     /gene="ybjG"
                     /locus_tag="OO572_004126"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6662898.1"
                     /translation="QRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHA
                     ADDSFPSDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGL
                     LAGMIGCLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
BASE COUNT          307 a          301 c          289 g          254 t
ORIGIN      
        1 cggacgcacc ttgccaaatt tactgtggtc gacaaccagc acatgctttt gcgccatcga
       61 catggcccag tgttttaccg gcaactcttc aagattaaaa caggtagcgc ctttactgac
      121 atgcacgccc gccgcggaat aaaaagcgat atccgggcaa aaattattca acgtttgctg
      181 aaaatcgatg ggtttgaaaa tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc
      241 gcggcaatgg ggtttctctt tcagcgccag aaaggtattt agcgaataac aaacggcggt
      301 aaaagggatt tcattatcaa tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa
      361 aaagagggtc tgatcgggtt ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc
      421 ttccaccagg cgggattttt gatcgcttaa caggtaatgg ctggcactgc gcggttccag
      481 aacaatatag ccgccgagca aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat
      541 cgtcatctcc gaaaccccaa gcagggcggc ggcgtcttta agatgtaact tatcgctgcg
      601 ttttaattct tgcagcagct gcccgatacg ctcttcgcga cgtgtttcca taatccctct
      661 gaatagttat tgaagcgagc cgctcaatac tacacttttt agcagagatc agtcacgcac
      721 ccagcctttg cggatcggta atgcaaaaca gacgcgatac cacgattgca gacgttgata
      781 gagtttatgc cccatcgctt gccagataat ctgggcacta aggcagccaa tcatacctgc
      841 cagcaatcca ccgagcatat ccagcggcca gtggacgcca agataaacgc gcgaccaggc
      901 aatgacgacg gccagcacca ttaaaagtga gccggaccac aggcgatgcc agcataaaaa
      961 tgccagtgca aaggtgaaaa tcaccgtacc gtgatcgctc gggaatgagt catccgccgc
     1021 atgatgcagg aagttatagc cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg
     1081 tcccatcgtc caggacacaa acaggctgac cgccagcgcg atagcgattt ttatcaccag
     1141 ttgccgttgt g
//
LOCUS       DAJTYL010000293         1149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYL010000293 DAJTYL010000000
VERSION     DAJTYL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.302"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(147..629)
                     /locus_tag="OO572_004127"
     CDS             complement(147..629)
                     /locus_tag="OO572_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6662899.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(620..922)
                     /locus_tag="OO572_004128"
     CDS             complement(620..922)
                     /locus_tag="OO572_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6662900.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          331 a          279 c          242 g          297 t
ORIGIN      
        1 tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc gtagagtaac
       61 gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca ggaaggagtg
      121 tccgttaaac tcaggctacc tcagactcag actttataaa acaaggtatt aggtgccaca
      181 actgactgaa caaaaccaaa acgctcataa aatgctctgg cgtcatcatc aattgcatgt
      241 accataacag ccttaatgcc aacctgatca gccagattga caacccggtg aatggcatca
      301 cttagtagcc atttaccgaa accatgcccc tgggtacaaa catcaacagc cagacgccct
      361 aacagaacaa cagggacagg attgggcatg ttatgccgca aactgcgacc aagctcggca
      421 tgagtgacgg aaccagtagc taatgagtag tatcccacaa tacgtaaagt atcctccaag
      481 caaataacga acgtgcgaga agcattgagc atctggtttt tcatagcccg accacgtaac
      541 cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc atccgtcaac
      601 aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga cgttcacgcc
      661 cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa cgttcgttat
      721 ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac gccagttcca
      781 tgatgaaatc ggtacggttc ttattgacca agctcgcagc ataatcaata acggctcgtt
      841 cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg acgcttgcag
      901 ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt tatcgcaaat
      961 ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat aaaaacaatg
     1021 gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg tctgattacg
     1081 gccgtttaat gtgattttga taatgaaatg cctgaaccac ggcagcctgg cggctgcaaa
     1141 tcggggtgt
//
LOCUS       DAJTYL010000294         1135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYL010000294 DAJTYL010000000
VERSION     DAJTYL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.303"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>1135)
                     /gene="yiaN"
                     /locus_tag="OO572_004129"
     CDS             complement(<1..>1135)
                     /gene="yiaN"
                     /locus_tag="OO572_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR6662901.1"
                     /translation="MFDVQIMAQTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVD
                     LPMKLVGHKPGGLGYVGVLAAMIMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAG
                     LIASGGIIAPIIPPSIPFIIFGVSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRL
                     NLPRQQKATMQEIWHSFVSGIWALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATV
                     IYREMTFATLWHVLIGAAKTTSVVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSP
                     RLLFIVIMVAILIVGMVMDLTPTVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLI
                     TPPIGNVLNVISGVAKLKFDDAVRGVFPYVLVLYSLLVVFVFIP"
BASE COUNT          344 a          292 c          287 g          212 t
ORIGIN      
        1 cgggaataaa aacaaatacg actaataatg aatataaaac caggacgtaa gggaaaacgc
       61 ctctgactgc atcatcgaat ttgagttttg ccacccctga aataacgtta agtacattac
      121 cgataggcgg tgtaattaag ccgattgagc agttaatgat aaacatgaca ccgaaataaa
      181 tcggatcgat tcctgcctct ttaactaaag gcattaatac cggggtaaga attaataccg
      241 tcggcgttaa atccatgacc atgccgacaa tcaggatagc caccataatg acgataaaca
      301 gcagacgcgg tgaatcgacc aacggttgca gtaaatcaga aaccatcatc ggcagttcag
      361 caatggtaat cagccaggcg gaaacttgtg ccgaggccac cagaaacata accactgagg
      421 tggttttcgc cgcgccaatg agtacatgcc aaagtgtggc gaaggtcatt tcacggtaaa
      481 taactgtggc gacaaacagc gcatagaaag cagcaacggc cccggcttcg gtaggggtaa
      541 acagaccgga gcgaaacccg ccaataatga tgactggaag aaacagcgcc cagataccgg
      601 agacaaaaga gtgccagatt tcctgcatcg ttgctttttg ctggcggggt aaattaaggc
      661 gactggcctg ccgccaccag gtaagcatca gcgttgcgcc catcattatg ccaggggcaa
      721 tgcccgccat aaacagctta ctgatggata atccgctgga aacaccgaag ataataaacg
      781 gaatggaagg gggaataatt ggcgcgataa tgccgccaga ggcaatcagc cccgccgccc
      841 ggttgaccgg gtagttggcg ctgcgcatca tcggcaccag tagagcggca acggcggcgg
      901 tatccgcaac ggcagaacca gaaaggctcg ccataatcat agctgccagc acgccgacgt
      961 agcccagccc gcctggttta tgccccacca gtttcatcgg caggtcaaca atgcgtttta
     1021 acaagccgcc cgcattcatg atttcacccg ccagcacaaa gaacggaatc gccaggaggg
     1081 agaagctatc ggctccgttc accagcgttt gtgccatgat ctggacatca aacat
//
LOCUS       DAJTYL010000295         1135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYL010000295 DAJTYL010000000
VERSION     DAJTYL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.304"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1077)
                     /locus_tag="OO572_004130"
     CDS             complement(<1..1077)
                     /locus_tag="OO572_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6662902.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRF"
BASE COUNT          305 a          306 c          251 g          273 t
ORIGIN      
        1 gaagcgccag ctgtacggga aaacttcaag catttgttcg gaatagccca tgtactcctg
       61 aacgccaaaa tttggtaccc ataggtcaaa atgcagcgcc gccgcatgac aaacaggcga
      121 taaatctgaa gggccatgcg aaccggtacg tacctgataa agcgatgcaa aatcggcaat
      181 ccgccgcatc ccggtaatgc cgcctgcatg ggtgatcgtc gtgcgaatat agccaatcag
      241 ctgctcttcg attaactgct tgcagtccca gatactgtta aacacttcac cgaccgcaat
      301 cggcgtcacg gtatgctggc gaatcagacg aaaacattcc tggttctcgg caggcgtcgg
      361 atcttccatc cagaacaggc ggtaatcctc aatgctcttc ccaaagcgcg ccgcttcgat
      421 gggggtcagg cgatggtgca tgtcatgcaa cagatgttcg ttaaaaccga acttattacg
      481 caccgcttcg aataacttcg gcgtaaaatc gagatacttt tctgaagacc acaactgctc
      541 ttctggccac aggcctttgg ttgccggttc atacgccagt cctttgccct tcgccaggcc
      601 ataagtggtt tgcatacccg gtacaccgca ctgcacacga atcgccttaa agcccatctg
      661 ctggtgcttt gcatagtctt caaggacttc atcaatcgta cgcccggtgg tgtggcaata
      721 aaccatcacg ccttcacgag aagcaccacc aagcagttga tacaatggca tatttgccgc
      781 tttagctttg atatcccaca acgccatatc aatggcagaa atggctgaca tcgtgacggg
      841 gccacgtcgc cagtaagcac ctttataaaa gaattgccag atatcttcga tacggctggc
      901 atcgcggcca attagttgtg gacaaagatg atctttcaga taagaagcga ccgacaattc
      961 gcgaccattt aacgtggcat ctcctaatcc acaaagtccg ctttcagtgg ttattttaag
     1021 cgtaacgaaa ttacgccctg ggcaagtaac aaaaacatct gcagcaataa ttttcattat
     1081 tgcaatctcc tgtgttgtaa atagacgttt caaacctaat tttacgccta ctacc
//
LOCUS       DAJTYL010000296         1132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYL010000296 DAJTYL010000000
VERSION     DAJTYL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.305"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..324)
                     /locus_tag="OO572_004131"
     CDS             complement(<1..324)
                     /locus_tag="OO572_004131"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6662903.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVT"
     gene            complement(457..762)
                     /locus_tag="OO572_004132"
                     /pseudo
     CDS             complement(457..762)
                     /locus_tag="OO572_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            770..1132
                     /gene="tnpA"
                     /locus_tag="OO572_004133"
                     /pseudo
     CDS             770..1132
                     /gene="tnpA"
                     /locus_tag="OO572_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          265 a          275 c          274 g          318 t
ORIGIN      
        1 tgtgactttg tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat
       61 tttgcgtttc tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg
      121 aagtctcgcc agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc
      181 atctgacagc gaggcaagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg
      241 gtgaaccgga gttgatactt cactttctgc ctgaatactg atgtaccact taccgcagga
      301 ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggctgttcc ggtagcgcat
      361 ccagccaagt tttggcagaa aaatacggct gttttcctga gcgagcttaa cgccctgcgg
      421 atagcggaat gcatcattct gtcaccgctt tttgaatcag gggaacgccg agcgcttctg
      481 gaaaaagttt ttgcaggcct gctcagggtc tttcagtgac tgttgcaatg gctgtgacgg
      541 agcatcttta atccattgcg tttcagtggc atttttccac tcaaccaatc aggaagccat
      601 tttcgcgtaa gggatgtctt tgttaccggc cttatgattc tcattctgac gtgccagagt
      661 acgactgaaa acgaaacgac atgcgcctgc gaagcgcctc atttcacgcg cctgttgacc
      721 accgggtctt aaccggaatt tgaatacctg tagtcgtttc acagggcaaa ttatactttg
      781 gtctgtgaaa aaaactgata tccgacgcgg aaggcactgt gttttcctga tgcatgctca
      841 ccgggtcttt gttaccaagt accgacgcca gatttttgac catgacgcga cagaaaaact
      901 acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg aaatggatgg
      961 cgaaccagat cacgtccatc tgttaattaa ctatcccccc aaactggcga tatccagtct
     1021 ggtaaacaaa cagcctcaaa ggcgtatcgg gtaggttact gcgacgggat cgaccagata
     1081 ttgctgccag gtattaccgc aaagatgttt tgtggcgtcc cggctatttt gc
//
LOCUS       DAJTYL010000297         1130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYL010000297 DAJTYL010000000
VERSION     DAJTYL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.306"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            13..>1130
                     /locus_tag="OO572_004134"
     CDS             13..>1130
                     /locus_tag="OO572_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6662904.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYL"
BASE COUNT          309 a          268 c          310 g          243 t
ORIGIN      
        1 cggagaactt aaatggccga cgaaaacaga ctcaattcca ttctgtgtaa gtttgacgca
       61 gactggatgg cgagcgatga agccagaacc gaggcgacaa atgacctgta ttttagccga
      121 gtgtcgcaat gggatgactg gctatcaaac tacactaccc tgcaatatcg cggacaattc
      181 gatgttgttc gcccggaggt caggaaactg gtcgcagaga tgcgccggaa ccctgtcgac
      241 gttctcttcc gacccaaaga cggcgctaat cctgatgcag ccgatgtatt gatggggatg
      301 tatcgtactg atatgcgcca taacacggca aagattgccg ttaacgttgg cgttcgtgag
      361 cagatagagt caggcgttgg tgcatggcgt ctggtcacgc agtacgaaga caacgatcca
      421 acaagcaaca atcaggtaat ccgacgcctg ccaatccatg aagcctgctc acacgtcata
      481 tgggacgcca acagcaagca gatggataag agcgacgcta agcactgcac ggtgattaac
      541 gccttgtcgc gcaatggctg gaaagagttc gcagaggatt acggtattga cccggatacc
      601 ctgccatctt tccagaatcc taacgataca tggctattcc cgtgggtgtc gaatgatgtc
      661 gtctacgtcg ctgagtatta cgaggtagaa gaggagaagg agaaggtctt catctaccgc
      721 gacccgctga caggtgagcc ggtcagctat taccagcagg atatcaaaga cgtcatcgac
      781 gacctggcta atcgtggatt cattaaggta gcagagcgca aggtgaagcg tcggcgtgtg
      841 tataagtcga tcatcacctg tacgcagata ctgaaagacc gcgagaagat agccggagag
      901 catattccaa tcgttccagt gtatggcgaa tggtcattcg ctggtgacaa ggagtgctac
      961 gagggtgtgg taaggctgac gaaagacggt caacgccttc gtaacatgat tatgtcgttc
     1021 aacgccgata ttgttgctcg gtcaccgaag aagaaaccga ccttcttccc tgagcaaatc
     1081 gaaggctacg aatacatgta cggcggaaat gatgactatc cgtactatct
//
LOCUS       DAJTYL010000298         1128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYL010000298 DAJTYL010000000
VERSION     DAJTYL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.307"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..864)
                     /locus_tag="OO572_004135"
     CDS             complement(<1..864)
                     /locus_tag="OO572_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6662905.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIV"
     gene            complement(1011..1087)
                     /locus_tag="OO572_004136"
     tRNA            complement(1011..1087)
                     /locus_tag="OO572_004136"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1051..1053),aa:Pro,seq:tgg)
BASE COUNT          289 a          304 c          272 g          263 t
ORIGIN      
        1 aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg gcgcaccgac
       61 aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat
      121 ccagtggtca aaaatcgcca ccagaaattc accaaattca tcggggcgca cagaccattc
      181 cgtcagttcc ccctgaatat caccaggcgc atgtagtttc agtccatcgc ggacagctgt
      241 ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc ccgcatcgca
      301 caaaaaatca taaacctgta acggttgctg cgcgctggtg cggttgacgc agaccagcac
      361 gttatagtcg acatgatgtt tttgcaggag cgtcagggca cgcatcacca gtttatgcgt
      421 gggtctgccg cctttggtca cgcgatattg attgtggatc tcagccggac catccagcga
      481 taacccaaca agaaaattat tttctgccag aaatgcacac cattcgtcat cgagcagcac
      541 gccgttagtc tggaaactgt tacttatctt cctgccagca ccatatttcg cctgtagcgc
      601 cacggcacgg cggtaaaact ccagcccgag tagcgttggt tcgccgccct gccaggtaaa
      661 agccacttcg ttttgcagtt cgctggcggc gatatagtga cggatatacg cttccagcgt
      721 gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt cgagataaaa
      781 acagtagtca cagttcagat tacaatcgga accgctcggt ttcgctatca catgaaaagc
      841 acgcgttgga acctgttgca gcatggtcgc tccttgcctg aagtcatcct tcaatacgtt
      901 aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc gaagggaggg
      961 tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga
     1021 gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca agctgcgcta
     1081 ctcgccgaaa tactgctttt tgaattttta gttcaattct ttaaagtc
//
LOCUS       DAJTYL010000299         1112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYL010000299 DAJTYL010000000
VERSION     DAJTYL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.308"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            57..554
                     /locus_tag="OO572_004137"
                     /pseudo
     CDS             57..554
                     /locus_tag="OO572_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            595..786
                     /locus_tag="OO572_004138"
                     /pseudo
     CDS             595..786
                     /locus_tag="OO572_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(880..>1112)
                     /locus_tag="OO572_004139"
     CDS             complement(880..>1112)
                     /locus_tag="OO572_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6662906.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          296 a          279 c          297 g          240 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgt aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat gacttcacgt tcgccaccgg agcaccgcag
      721 aagcagctcc agtcgttacg ctcactcagc ttcatagagc gcaatgaaaa catagtctta
      781 ctggggacat ctgacatcac aaatccccgg gtggggattt gtgtataaga gacagcccat
      841 ggaggaagga gatatatcgc agctctgacg actcactcct tatttacccg ggaaatactt
      901 ctccagcaat gcatacagga tggcagcggt ttcagcatcc attatgccgt cataattttt
      961 ctgccggaaa tgaagctgaa aagcgcggac aagcgcctta tatccgtcat ccgtctccac
     1021 accagaaata ctgtatccat aggtcttcag ccgtgacacg atatcaacct tatccggaat
     1081 gccctgcgat tcaaaaaata aatatttagt ca
//
LOCUS       DAJTYL010000300         1057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYL010000300 DAJTYL010000000
VERSION     DAJTYL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.309"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..952)
                     /gene="treF"
                     /locus_tag="OO572_004140"
     CDS             complement(<1..952)
                     /gene="treF"
                     /locus_tag="OO572_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6662907.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDL"
BASE COUNT          246 a          271 c          284 g          256 t
ORIGIN      
        1 gtaaatcgcg gtacacctcg ttgggcgggc gaccagaatg tttcgcggtt tcaacgtcct
       61 caagccagga ttcgtcacgc ggcgtgtcgc gatcgtccca gtaacggttg agcagcgatc
      121 cgtccggcat ccgcacaaca tggcgatagg cctgattagg gatcaacgat tctgcaccgt
      181 ccatccagaa ggcatattcc attttaaggt ggtcgagata gcggcgcgca ccgcgtacac
      241 catcttcttc aaacaactcc accatcagcg caaaaaccgg tggttgcgag cggctcaaat
      301 aataggtgcg gttgccgttg gggatgtgac cgtagttttc gatcatccag gcgaagttat
      361 cggccatgca tttcagcaaa tcttcccgac cactttccgc cagtcccagc atggtgaaat
      421 aggaatccca atagtaggtt tcgctaaaac ggccgcccgg gacaatatat gactgcggta
      481 gcgccagcag agaagaccac ggaatgtgat cctgtggttc gcgggttagc accggccaca
      541 gctggtcgat atgctctttc agggaatttt gcgggtccga tacatactcg ctggagtaga
      601 cctccggcag ccagaagtga ttttcaacaa acttgcgcaa gtcaaaatca cgatgacggc
      661 gcactttacg gtagcggatt aaaatatcca gcgggtccat tttgggtgcg cagtcgggaa
      721 aggttttact gtcggggaaa attttcgccg actgaacatg ctcgaacagt tcgagatagc
      781 gatcggcagg cgtcagcgca tcagaggcgg gcagcccttc aatcatctcg ggttccggtt
      841 ctgcctcgat catctcatcc agttttaatt catacgggtc cagttcatag cagagatcga
      901 cctcgatcat cagttcgtct ggattagggt tttgaatttt ctgattgagc ataaggatcc
      961 aacctccgaa attcgtcgct aattatcggg cttttcttta agggtagtca ttcgctccgc
     1021 ctggtgggcg acaaagcgtg cggtggatca cgtttat
//
LOCUS       DAJTYL010000301         1038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYL010000301 DAJTYL010000000
VERSION     DAJTYL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.310"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..1009
                     /locus_tag="OO572_004141"
                     /pseudo
     CDS             1..1009
                     /locus_tag="OO572_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          243 a          264 c          307 g          224 t
ORIGIN      
        1 ctggcgcagt aaacagcagc aacaaatgag ttcgtcagag cgcgaaagcg aactggccgc
       61 tgaaaatgtc cgccttaaac gacaactggc ggagcaggct gaggaactgt ccatcctcca
      121 aaaggccgcg acatacttcg cgaagcgcct gaaatgaagt atgtctttat cgaaaatcat
      181 cgggcagagt tcagcatcaa agcgatgtgt cgtgtacttc gggttgcccg cagcggctgg
      241 tatgtctggc tcaggcgtcg tcaccagatg agcctgcgcc aacagtttcg gctcacctgc
      301 gatgccgctg tccataaggc attctttgag gcaaagcagc gatacggtgc tccccgcctt
      361 gctgacgaac tgccggagtt caatattaaa accattgccg ccagcctgcg tcgtcagggg
      421 ctgcgggcga aagccagccg gaagttcagc ccggtcagct accgtgcaca tggcctgccc
      481 gtattggaga atctgctgga gcaggacttc agcgccagtg gcccgaaacc agaagtgggc
      541 gggtgacatc acgtacttgc gtaccgatga gggctggttg tatctcgcag tagtcatcga
      601 cctgtggtca cgcgccgtca ttggctggtc gatgtcaccg cgaatgacag cacaactggc
      661 ctgtgatgca ctgcaaatgg cgttgtggcg gagaagacgc ccggaaagcg tcattgttca
      721 tacggaccgt ggtggtcaat actgttcagg ggattatcag gcaatgctga agcgacacaa
      781 cctgcgtggc agtatgagtg cgaaaggcta ctgttatgac aatgcctgtg tggaaagctt
      841 ctttcattcg ctgaaggtgg aatgtatcca cggggaacgc tttagcagcc gggaaataat
      901 gcgggcaacg gtgtttaatt atatcgagtg tgattacaat cgctggcgtc gtcacagtgc
      961 ctgtggcggt ctcagcccgg aacaatttga aaaccataat ctcgcttagg gccgtgtcca
     1021 cattacgtgg gtaggatc
//
LOCUS       DAJTYL010000302         1036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYL010000302 DAJTYL010000000
VERSION     DAJTYL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.311"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..225
                     /locus_tag="OO572_004142"
     CDS             <1..225
                     /locus_tag="OO572_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662908.1"
                     /translation="EVNLEGKRIGYVIKTENKETPFTVVDIDGPSGNVKTLNDGVKKM
                     CLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            325..>1036
                     /locus_tag="OO572_004143"
     CDS             325..>1036
                     /locus_tag="OO572_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6662909.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKE"
BASE COUNT          326 a          236 c          237 g          237 t
ORIGIN      
        1 gaggttaatc tggaaggaaa gcgtattggc tacgtgatta agacagaaaa taaagaaacc
       61 ccattcactg tggttgatat cgacggtcca tcaggcaacg ttaaaacact taacgatggt
      121 gtaaaaaaaa tgtgtctggt gcacatagga aagaatctgc ccgcagaaaa aaaagccgaa
      181 tttctggcaa ctctgattgc aatgaaatta aaaggtgaaa tctgaaaaaa agaaagcctg
      241 cacactgtgc aggcctgaat gaagaacctg ggacatttat tcatcactcg cagtaatttt
      301 aatctgagtt gaggttaaaa aacaatgaac accgataaac aagtttaccc actgtattac
      361 gaagcaaaaa atgacaaagt aagaaaacgt ctcggtatta aaggcggttt ttactgggct
      421 gaagcgaaaa aattatccat tgccatctcc cgtggtgctg ttgcgattga cgatgcaggc
      481 tacgatgaag atgactttaa aaaacctgtt cgcgtcaatt tgcccgttgt tgatgacctc
      541 ccgccagaag gcgtatttga tacggaattc tgcaaccgtt acgaaaaagg cggggaagat
      601 ggcatcacaa tggtatttat cgcatcttct ccctctgttc aggacaaacc ggccagcact
      661 gacaatacca acgtcaacgg cgaagacatg acagaaattg aggagaacat gctcctgcca
      721 gtttccggtc aggaactgcc cattcgctgg cttgcgcaac acggcagcga aaaaccagta
      781 acgcacgttt cacgcgacga actccaggca ttacacattg ctcgggatga agaactacca
      841 gctgttactg ccctggctgt ttcccacaaa accagcctgc tcgatccgct ggaaattcgc
      901 gatctccaca aactggtgcg tgatactgac agagttttcc ctaatccagg caattcaagc
      961 ctggggctga tgactgcttt tttcgaagca tacatggacg cagactacac cgatcgcggt
     1021 ctgctgacaa aagagt
//
LOCUS       DAJTYL010000303         1031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYL010000303 DAJTYL010000000
VERSION     DAJTYL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.312"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..443)
                     /locus_tag="OO572_004144"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="OO572_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(491..1030)
                     /locus_tag="OO572_004145"
     CDS             complement(491..1030)
                     /locus_tag="OO572_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6662910.1"
                     /translation="MASLMLAGHALAEDGVVNFVGEIVDTTCEVTSDTADQIVPLGKV
                     SKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGTPLTAGNP
                     GDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARYVATNA
                     TVTAGTANADSQFTVEYKK"
BASE COUNT          283 a          237 c          214 g          297 t
ORIGIN      
        1 cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga tggcggaata
       61 tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac acctttggtg
      121 tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg cggaataacg
      181 gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc ctggatgaga
      241 taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt attacttaag
      301 taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg ttggcctaaa
      361 aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt aaagagcctg
      421 attaaacaac tttttctgga cataacgact ccctggccaa agccaactga ataaaaaggc
      481 ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc gccgtaccag
      541 ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca gccttgcctt
      601 cagcatcgat agcggtataa gcagagtcgt tataaagttt aacctgcgaa ttatcggact
      661 ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta ccggtaaaat
      721 caccagggtt gcctgctgta agcggcgtac ccactttcag gtcgccgtcg ccgccaggcg
      781 cttctgtacc atcaaaacga acggctgctt gagtgtacgt tgccgggcaa ttttcaagtt
      841 taatactgaa cttctgtggc gacgccagac tacctacacc tgaaaatgca tttttactga
      901 ctttacccag tgggacaatt tgatcggctg tatcggaggt aacttcacaa gtagtgtcga
      961 caatttcacc gacgaagtta acaacaccat cttctgccag tgcatgcccg gccagcatta
     1021 gagatgccag a
//
LOCUS       DAJTYL010000304         1013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYL010000304 DAJTYL010000000
VERSION     DAJTYL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.313"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..116)
                     /locus_tag="OO572_004146"
     CDS             complement(<1..116)
                     /locus_tag="OO572_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6662911.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIV"
     gene            254..>1013
                     /locus_tag="OO572_004147"
     CDS             254..>1013
                     /locus_tag="OO572_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6662912.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQA"
BASE COUNT          285 a          249 c          235 g          244 t
ORIGIN      
        1 actatctggc ttaaagctga ctgcaccatg ctcaggcggg ctgctgcgcg agtgaagcta
       61 cgctcctctg caacgacgac aaacgccatc agatcagcga tttcttcacg cttcattcag
      121 tatctccata caatctatta atcatttaaa tgataggtct aatcatgatt tagggtctaa
      181 tcaacttaat gatcaatatc tatagtgata tcaccaaccg tcaaaacgaa ccgacaactc
      241 catgaggaac tgaatgatga ataataaagt cagcttcact aacagcaata atccaaccat
      301 ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc agtatccggc
      361 aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa cctatgccga
      421 aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc aaggtgaaag
      481 tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg tcagtgccgt
      541 gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg cgatgggaat
      601 ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca tcaaggccat
      661 cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg aaaacaatgt
      721 gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa ccagcgatat
      781 tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg atgcgccgaa
      841 cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt atccaacagc
      901 accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg cttaccatat
      961 ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg cag
//
LOCUS       DAJTYL010000305          997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYL010000305 DAJTYL010000000
VERSION     DAJTYL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.314"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            31..780
                     /locus_tag="OO572_004148"
     CDS             31..780
                     /locus_tag="OO572_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6662913.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          280 a          213 c          218 g          286 t
ORIGIN      
        1 aaatatgtat cctctataag gagatgttat atgaaaagac agctttttac tcttagtatt
       61 gtcggggtat tttctttaaa tacctttgca tcattccctc cgggaaatga tgtgacaaca
      121 aaacctgacc tttactacct gacaaacgat aatgctattg acagtctggc attattaccg
      181 cctccaccac aaatcggaag tattgccttc ctgaatgacc aggccatgta tgaaaagggg
      241 cgtttattgc gaaatactga aagagggaag ctggcagctg aggatgccaa tctcagtagt
      301 ggtggcgtgg caaatgtatt ttctgctgct tttggttctc cgataacggc taaagattca
      361 ccagaattgc ataaattact gacaaatatg attgaggatg ccggagacct ggcaacgcgt
      421 tcagcaaaag aatattatat gcgtattcga ccttttgctt tttatggtgt ttctacgtgt
      481 aatacaaaag aacaggatac attatccaga aatggctctt atccatcagg tcatacatcg
      541 attggttggg caacagcact tgtattgtcc gaaataaatc cggcacgcca ggataccatt
      601 ctgaaacggg gttatgaact gggggacagc agggttattt gcggttatca ctggcaaagt
      661 gatgttgacg cagcacgtat tgttggctct gctattgtag caacactgca ctcaaatcct
      721 gtgttccagg cacaattaca aaaagcgaaa gatgaattcg ctaacaatca gaaaaaataa
      781 tgcttttagc cagtacgcct tataaccccg accttcaggg ctaatgccag tcagttaagc
      841 aactgactgg ctctttttca gggctgtggg gtatttccag tgcctctcct ttaccactct
      901 cggaaaggcc ctttcccttc ttgtcggtaa tttcactagg ctgtgtccct taattacgta
      961 agcgttgata aaagagtcgg atgcagccca gtttcac
//
LOCUS       DAJTYL010000306          975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYL010000306 DAJTYL010000000
VERSION     DAJTYL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.315"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>975)
                     /gene="arsB"
                     /locus_tag="OO572_004149"
     CDS             complement(<1..>975)
                     /gene="arsB"
                     /locus_tag="OO572_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6662914.1"
                     /translation="QPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFI
                     AVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILT
                     PIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYAS
                     VMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLL
                     VGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVV
                     YGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVL"
BASE COUNT          272 a          286 c          256 g          161 t
ORIGIN      
        1 agtaccgtcg gcatattgtt cataatagaa gagaggaagg cggtgaggaa tccggtgccg
       61 agcgtcgcgg cccacaggcc gttatccgcc agcacgttga gtacgccaga aagatattcc
      121 gttaatccgg cattgcgcag gccataaacc accagataca tgccgagcga gaagatgaca
      181 atctgccagg gggcaccgcg caggacttta cccgtattaa tcgcatgacc gcgtttagcg
      241 acgacaaata atatcagtgc gcccacagct gcaatggcgc tcaccggaat gccgagcggt
      301 tccaggacga aaaatcccac cagcagaagc agtaaaacaa cccagccagt tttgaacgta
      361 gcaggatctt tgatcgcttc tgcgggagat ttcagcagcg ccatatcgta gttctgcgga
      421 atatctttgc gaaaatagag atgtagcatc accagcgtgg caacaatcgc ggcgatatcc
      481 accggcacca tcaccgaggc gtattcgcga aagccgaggc caaagaaatc agcggaaacg
      541 atattcacca ggttggagac aataagcggc aggctggcgg tatcggcaat gaatccggcc
      601 gccatcacga acgccagcgt cgtgccttta ctgaatccta aagccagcag catggcgatg
      661 acaatcggcg tcaaaataag cgccgcgcca tcattggcaa acagggccgc aacggcggca
      721 ccgagcagga caatccaggt aaacagcaag cgaccgcgtc cattgcccca gcgtgaaacg
      781 tgcagcgctg cccattcaaa aaagccggac tcatccagca gcaggctgat gataatgacg
      841 gcaataaacg ccgccgtcgc gttccagacg atattccaca ccaccggaat atcacccgga
      901 tgaaccacgc ccgtaaccaa cgccagtacc gcgccgagcg ttgcactcca gccgatgcct
      961 aaacctttcg gctgc
//
LOCUS       DAJTYL010000307          973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYL010000307 DAJTYL010000000
VERSION     DAJTYL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.316"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(257..>973)
                     /locus_tag="OO572_004150"
     CDS             complement(257..>973)
                     /locus_tag="OO572_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662915.1"
                     /translation="RIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANLALAEAV
                     SAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVEL
                     AEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDG
                     IKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGG
                     SLAALTSPLISTTPVAENVK"
BASE COUNT          187 a          259 c          244 g          283 t
ORIGIN      
        1 gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg gaggggcttt
       61 cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct cgcgcaacgc
      121 tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca acgacctgat
      181 catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg tgccagggac
      241 gttcatagct gaaaatttat tttacgtttt cggcgacggg agttgttgag attaagggtg
      301 aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc attgagcaag gagtcaatca
      361 gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgct aggtttccgc
      421 cccccacatt acccgtgttt gcatcacccg cagcgctgcc actattcaga ccatcgacct
      481 gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct attaccgcag
      541 gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca gaaagtacgt
      601 tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt ttatgtgtag
      661 cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt tctgctttcg
      721 cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg attagggcaa
      781 cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt tgttgcgcac
      841 ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt tgttccgatt
      901 tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc tgctgttcga
      961 tctctttaat acg
//
LOCUS       DAJTYL010000308          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYL010000308 DAJTYL010000000
VERSION     DAJTYL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.317"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            30..722
                     /locus_tag="OO572_004151"
     CDS             30..722
                     /locus_tag="OO572_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6662916.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAAIAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            732..>967
                     /locus_tag="OO572_004152"
     CDS             732..>967
                     /locus_tag="OO572_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6662917.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQFIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQA"
BASE COUNT          257 a          220 c          269 g          221 t
ORIGIN      
        1 atcgacttcc tgaatcacgg gagatagcca tgttatatgc atttaagctg ggcagaaaac
       61 tgcgcggcga ggaaccttat tgccctgaaa aaggcgggaa aggtggcagc tctgataaaa
      121 gtgcaaagta tgcagcagaa gctcagaagt atgccgcaga cctgcaaaat cagcagtggc
      181 agacgatcat gaaaaacctt gctccgttca cgccgcttgc ggagcagtat gttaaccagc
      241 ttcagaacct ttccagttta gaaggacagg gtcaggcact taatcagtat tacaactctc
      301 agcagtataa agaccttgca ggtcaggctc gttaccagag tcttgctgct gcggaggcta
      361 cgggaggact tggttcgaca gccacaagca atcaactggc tacgattgct ccgactctcg
      421 gtcagtcgtg gttatcaaac cagatgagca attacaataa tctggcaaac gttgggcttg
      481 gtgcgctgca aggtcaggca aacgctgggc agacatacgc caacaacatg agcagcattg
      541 cacagcaaag tgcagcaatt gccgctgcta atgccaataa accatcaagt cttcagactg
      601 caattagcgg tggcacgtct ggtgcgattg ccggtgcagg tcttgccagc cttttgggaa
      661 catcaacacc ttggggcgct ggcattggtg ctggtatcgg attgcttggc tcgttgtttt
      721 aaggggtaat catggctact tggcaaggat caaatggtgg attgttggct ggtatcggcg
      781 gcgtaaactc aaacgctccg agcgtaaatg acatcggcaa tacgcttcag tttatcaggc
      841 agaacaatga tattgagcgt tcaggcgcta acaatgttgg gctgactgct ttgcaaggcc
      901 tttcaggtat tgcgggggtg tttcagcagg aaaagcaggc tcagcggcag aaagaatttc
      961 agcaggc
//
LOCUS       DAJTYL010000309          944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYL010000309 DAJTYL010000000
VERSION     DAJTYL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.318"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..944
                     /locus_tag="OO572_004153"
                     /pseudo
     CDS             1..944
                     /locus_tag="OO572_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          252 a          236 c          272 g          184 t
ORIGIN      
        1 ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa
       61 agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa agctacaacg
      121 cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt cagactccct
      181 gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac actctgccat
      241 gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga aaaaccagac
      301 ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag ccacggttcg
      361 gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat gggacgctgg
      421 cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc gactcaccgg
      481 tataaacgtg gtggtcatga acatgttgct atccctaact accttgaaag gcagttcgcc
      541 gtgaccgagc caaatcaggt gtggtgcggt gatgtgacct atatctggac gggtaagcgc
      601 tgggcgtacc tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg ctgggccatg
      661 tcgttctcgc cggacagcag gctcaccatg aaagcgctgg aaatggcatg ggaaacccgt
      721 ggtaagcccg gcggggtgat gttccacagc gatcagggca gtcattatac gagcaggcag
      781 ttccggcagt tattgtggcg ataccagatc agacagagta tgagccggcg cggaaactgc
      841 tgggataaca gcccaatgga acgcttcttc aggagtctga agaacgaatg gatgccggtg
      901 gtgggttacg taagcttcag cgaggcagct cacgccataa cgga
//
LOCUS       DAJTYL010000310          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYL010000310 DAJTYL010000000
VERSION     DAJTYL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.319"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..258
                     /locus_tag="OO572_004154"
     CDS             <1..258
                     /locus_tag="OO572_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662918.1"
                     /translation="LDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSG
                     DGQAVRIQFDSIINSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            382..624
                     /locus_tag="OO572_004155"
     CDS             382..624
                     /locus_tag="OO572_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6662919.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            628..774
                     /locus_tag="OO572_004156"
     CDS             628..774
                     /locus_tag="OO572_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662920.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            783..>932
                     /gene="xisR"
                     /locus_tag="OO572_004157"
     CDS             783..>932
                     /gene="xisR"
                     /locus_tag="OO572_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6662921.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDG"
BASE COUNT          248 a          198 c          253 g          233 t
ORIGIN      
        1 ttggatcgca tcattccgaa aaagtttgac cgctgtactc acccggtgct ccaggcgggt
       61 tacctgagtt atccagagaa gatgtttggt cgtgagcgta aatttattcc cgtccagtta
      121 cgtccctccg gtgacgggca agcggtcaga attcagtttg attccatcat caactcaatg
      181 tatggcaatc ctgaatttgt tgtgatgcct tgtcgtgatc atggcgattt caacgtggct
      241 caggagcatc cggaatgaaa atcgaatacc aggacgccgc aggaggtgaa tcaatgagct
      301 ggcctgatgc aatcgtaact ctggggatgg tattcgcagt agcgtttgtt gtgtactcga
      361 tttgtcgatg gggataacca catgttcgct ttgattcaac gcgggcagat atacacagac
      421 agagccggat acccagtaga aattactcgc agtactgagc actcagtgtt ctttcggagg
      481 atggacggac gtaccgggcg agtacgcatc ggtgaattca gtagcctgtt cgagcatatt
      541 gaccacctgg aatatcacaa gattctggct gaaacagaac aagaaaagca cctgaagaaa
      601 ttacgcgcaa tgcaaaggaa gtaaaaaatg aataaagcgt ttgagctatg ggtccaccag
      661 cgttacggca accactacga cctgacgcga gatgttgacg gtttctactg tcgtgaaatt
      721 gtgaaacgaa tgtttgaggt ctggtgccac tgccgtgggc tgagtgttgt gtgaggtaat
      781 gcatgggcaa tgtgattcaa ctggctccca atgaatgggt ttgtgaaagc gttctaatcg
      841 caattaccgg gctcaaacca ggcacaattc ttcggacccg gaaagaatgc tggatggttg
      901 gaagagagta tattcacgta tcaccagacg gt
//
LOCUS       DAJTYL010000311          930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYL010000311 DAJTYL010000000
VERSION     DAJTYL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.320"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..293)
                     /locus_tag="OO572_004158"
     CDS             complement(<1..293)
                     /locus_tag="OO572_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6662922.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDP"
     gene            complement(290..652)
                     /locus_tag="OO572_004159"
     CDS             complement(290..652)
                     /locus_tag="OO572_004159"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6662923.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          281 a          196 c          188 g          265 t
ORIGIN      
        1 gggtcttgca ggtcaacatc aattggaatc acggcatcgc cggttgtatg gtctaaccct
       61 gcaaacaatg ctggttcttt accaaagttg cgtgtaaatg acagcggaac aactagcgga
      121 tcagaaacag ccagagcgtt aatgattgac tccgtagcgt ctttgcttcc gtcatttatg
      181 aaaacaattt ctacttcata tggcttcaac tcttcgaatt cacgtaccgt tttataaaaa
      241 attggtatcg cttcttcttc attgaagaca ggaacgacaa gagatatctt catttcgcat
      301 ccctaaagac aatgaacttt gaatagacga aaccgcacac caagctgatg gcggagaagg
      361 taacaagagt gacaattgga ggaagtgaac atctatcagc aacccatcca acagtagcac
      421 tcagtgttcc catgaacccg acatataaca tgtagcgcat cgttgtagtt gatgctttga
      481 atgtgaattt tgcattcgca aagaagctaa aactcacagc cacaacgaaa cctgcgaagt
      541 ttgccatagc ctgattggta tgcgcggcat agatacatac accaaaaacc acccagtgta
      601 taagtgtgtt cagcacgcct atagatgtgt actttgcaaa tagctttaac atttcttcta
      661 tcagctaata atcaaaggca tgaagtctat catccaagtc tcaatcgatc gatacttgcg
      721 gtagttgatg agaaaaactc tggcacacaa atctttgcac tggattgcaa ggctttgtgc
      781 tcttctgtga atgtgtggct atatgtttga agatcgttgt gccgtatttg tgacatatat
      841 atggcaacat cattcatcaa ctttctgttt gtgccatcaa ctatatctta gtgaatgcgg
      901 ttaatgcttg ctaaaacaga tagttatgat
//
LOCUS       DAJTYL010000312          903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYL010000312 DAJTYL010000000
VERSION     DAJTYL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.321"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..903
                     /locus_tag="OO572_004160"
                     /pseudo
     CDS             1..903
                     /locus_tag="OO572_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          217 a          250 c          256 g          180 t
ORIGIN      
        1 gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag tgaagccctg
       61 aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct gtatgaatca
      121 cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga acgtgaactg
      181 gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga tgaagagctg
      241 gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag tatgtcttta
      301 ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc cgggtggccc
      361 gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt cagcagttcc
      421 gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag cgttacggtg
      481 ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta aaaaccgtgg
      541 cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc agcccggtca
      601 gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat ttttacgcca
      661 gtggcccgaa ccagaagtgg ccaggagaca tcacgtactt acgtacagat gaaggctggc
      721 tgtatctggc agtggtcatt gacctgtggt cacgtgccgt tattggctgg tcaatgtcgc
      781 cacgcatgac ggcgcaactg ccctgcgatg ccctgcagat ggcgctgtgg cggcgtaaga
      841 ggccccggaa cgttatcgtt cacacggacc gtggaggcca gtactgttca gcagattatc
      901 agg
//
LOCUS       DAJTYL010000313          883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYL010000313 DAJTYL010000000
VERSION     DAJTYL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.322"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..82
                     /locus_tag="OO572_004161"
     CDS             <1..82
                     /locus_tag="OO572_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6662924.1"
                     /translation="DMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            82..432
                     /locus_tag="OO572_004162"
     CDS             82..432
                     /locus_tag="OO572_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6662925.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          296 a          204 c          208 g          175 t
ORIGIN      
        1 ggatatgact ctcggttgga tcgtctggca gcaacgagct ggtatccatt tctcaacaat
       61 gtcacagcaa gaggtgaaat aatggagcca tacagcctca cactcgatga ggcctgtcat
      121 tttctcaaga tatccagacc gactgccatt aactggatac gcacagggcg tcttcaggca
      181 acacgcaaag atcccactaa gaataaatct ccttacctca caacacgaca agcctgcatt
      241 gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag
      301 gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga
      361 acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc
      421 atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa
      481 cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg
      541 atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatacaa ctaagagaca
      601 ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa
      661 actggaaact cagggcagaa tcatgcagaa aaaaagggaa acctgttcca gaatacacgc
      721 caaaaccagc gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa
      781 gagccgcaga gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac
      841 caaagaataa acggatccgc tggctggagc cccatgaagc aca
//
LOCUS       DAJTYL010000314          872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYL010000314 DAJTYL010000000
VERSION     DAJTYL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.323"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            71..343
                     /locus_tag="OO572_004163"
     CDS             71..343
                     /locus_tag="OO572_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6662926.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(309..716)
                     /gene="nudG"
                     /locus_tag="OO572_004164"
     CDS             complement(309..716)
                     /gene="nudG"
                     /locus_tag="OO572_004164"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6662927.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            803..>872
                     /locus_tag="OO572_004165"
     CDS             803..>872
                     /locus_tag="OO572_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6662928.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDK"
BASE COUNT          217 a          230 c          211 g          214 t
ORIGIN      
        1 tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa
       61 ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact
      121 ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc
      181 aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc
      241 tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac
      301 ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga
      361 atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc
      421 tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg
      481 ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg
      541 atgtccaatt cttcgtttaa ctcacgcacc agcgcctgct gctggctttc atccagctcg
      601 actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg
      661 agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat
      721 tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag
      781 gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt
      841 gttgcatcag tgcgtgcggg ttcttgataa ac
//
LOCUS       DAJTYL010000315          843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYL010000315 DAJTYL010000000
VERSION     DAJTYL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.324"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            50..361
                     /locus_tag="OO572_004166"
     CDS             50..361
                     /locus_tag="OO572_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6662929.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            345..503
                     /locus_tag="OO572_004167"
     CDS             345..503
                     /locus_tag="OO572_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6662930.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            496..>843
                     /locus_tag="OO572_004168"
     CDS             496..>843
                     /locus_tag="OO572_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6662931.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNA"
BASE COUNT          238 a          189 c          195 g          221 t
ORIGIN      
        1 tatgagatcg actccctacg atttcattca gttaatcaaa gaagtcataa tgcctggaaa
       61 aacagccgct ctatacgatg tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac
      121 tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa gccgttgggg tagatttaag
      181 cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt gtgcttgata accaacgtcg
      241 ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa ctcatcgctg aacctgctca
      301 accgacgctc gccctcaagg cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg
      361 aagagattac tctgcccata gtcctctcct gtaattatca gtctgatatt acttatcctg
      421 ggcaaaaaca gtttgattgc ggtaaccctg ttatcgataa atttgtacgc gctacgcgca
      481 tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat tgacagacaa
      541 agtggtgaac tgattggcat ctgtactttt acggcatatt cgctggaaag acaacgcgtt
      601 tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt agtcatgttg
      661 gggggagcgc ggaagtatca aaagcggggc tttggtcagg acctactatg tgattttttt
      721 gaacatgtaa aaaaaattca cccggcatta ccaattaaag gggtttatct tgatgctgac
      781 cctgccgcca ttaactttta tgctcgtctc ggctttgttc agctttcagc gacaccaaat
      841 gct
//
LOCUS       DAJTYL010000316          839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYL010000316 DAJTYL010000000
VERSION     DAJTYL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.325"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            123..566
                     /locus_tag="OO572_004169"
     CDS             123..566
                     /locus_tag="OO572_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6662932.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
BASE COUNT          268 a          174 c          196 g          201 t
ORIGIN      
        1 aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt cgactgttaa
       61 ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg ttcggccttt
      121 gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct atgagatggg
      181 aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca aaaattcgca
      241 tttctctccc caccacgatt tccagattga atttaacact tgccgatgta ctggataact
      301 tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc aggacaattg
      361 cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt gacccaccgc
      421 catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa ggcagtgatt
      481 tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac cgtgacggac
      541 gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa tgccacttgt
      601 aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa tgctcctttg
      661 catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta
      721 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
      781 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcac
//
LOCUS       DAJTYL010000317          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYL010000317 DAJTYL010000000
VERSION     DAJTYL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.326"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            33..823
                     /locus_tag="OO572_004170"
                     /pseudo
     CDS             33..823
                     /locus_tag="OO572_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          212 a          206 c          219 g          186 t
ORIGIN      
        1 agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat cccgaagagt
       61 ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc agcgttgcaa
      121 cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac ggtccggatt
      181 cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc cagaaagagc
      241 tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac ttcgcaaagc
      301 tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg ttcgcctgct
      361 ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc agccgcattc
      421 acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct ggctggaatc
      481 gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg ggcaacagtg
      541 cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc aggtcggata
      601 ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca ggctccagcg
      661 acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct acatcaggac
      721 ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca aaattatcgg
      781 ctggtccatg caatcccgga tgacaaagga cattgtcctg aac
//
LOCUS       DAJTYL010000318          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYL010000318 DAJTYL010000000
VERSION     DAJTYL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.327"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            116..>823
                     /locus_tag="OO572_004171"
     CDS             116..>823
                     /locus_tag="OO572_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6662933.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPE"
BASE COUNT          279 a          203 c          116 g          225 t
ORIGIN      
        1 atatatccgc ctcctccaga agttcctgtc tactgctgaa gggaaccaat aacattgcaa
       61 agcagataaa aaagaatcca acacctctca acataaagaa tacaccctga tatttatgct
      121 cccgacaaat aacaatcaca gattaatttc aaattcgttc tccacttatt caatcgacac
      181 tagccgcgca tatgaaaatt atctaaccca ttggactgaa tggaaaaata accgcataca
      241 agaagaacaa cgagacatcg cttttcagcg actagtatca tgtctacaaa accaagagac
      301 gaacctggac ttgtctgaat taggcctgac aacattacct gaaatccccc cgggaattaa
      361 atcaattaat ataagtaaaa ataatttaag cttaatctcc ccattgcctg cgtcccttac
      421 acaacttaat gtcagctata acagacttat tgaactgcct gctttgcctc aaggacttaa
      481 attattgaat gcgtcccaca atcaactaat cacactaccc acactcccca tatctttgaa
      541 ggagcttcat gtctcaaata atcaattatg ttctcttcct gttttaccag aactactgga
      601 aacattagat gtatcatgta atgggctggc agttttacca cctttaccat tttctttaca
      661 agagattagc gcaataggga atcttcttag tgaactcccc cctctacctc acaacattca
      721 ctccatatgg gcaatcgaca atatgttaac cgatattcca tacctgccgg aaaatttaag
      781 gaacggttat tttgacataa atcagataag tcatatcccg gaa
//
LOCUS       DAJTYL010000319          821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYL010000319 DAJTYL010000000
VERSION     DAJTYL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.328"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..757
                     /locus_tag="OO572_004172"
     CDS             <1..757
                     /locus_tag="OO572_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6662934.1"
                     /translation="DSELTRRYEYARLDTENIYLLPLARGNNHNYDGKSVVEIRKLNI
                     GKQSWPFNYVTDTCREFNGITTTGRMLYRNLKITSALDEIYGGICKKAHAATELAEGL
                     RLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTNEQPRI
                     DIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIGIEAYPGTAEIHSKMGYNVIP
                     GDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
BASE COUNT          270 a          159 c          166 g          226 t
ORIGIN      
        1 cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa atatctatct
       61 tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag
      121 aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata catgcagaga
      181 attcaatggt attacaacaa caggacgtat gctctatcga aacttgaaaa tcacctcagc
      241 tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag agttagcaga
      301 aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg actatacagt
      361 tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa ccacgatagg
      421 atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc agccaagaat
      481 tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt ccggattcgc
      541 ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca ttggtataga
      601 agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta tccccggtga
      661 tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag aactgtttga
      721 attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa tgagtactaa
      781 tattcaggtt atacagatat ttaaacaaaa ccctgcaatt t
//
LOCUS       DAJTYL010000320          810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYL010000320 DAJTYL010000000
VERSION     DAJTYL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.329"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..772
                     /locus_tag="OO572_004173"
                     /pseudo
     CDS             1..772
                     /locus_tag="OO572_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
BASE COUNT          195 a          228 c          211 g          176 t
ORIGIN      
        1 gttttttgcg ggtgagaacg gtaactccac cactggcgac gccatttata tgcgtggtgc
       61 cgatacctct aacagtattt atattgatgg tattcgcgat atcggcagcg tctcgcgcga
      121 caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg actacgggcg
      181 cagcgcaccg acaggctcga tcaatatgat cagcaagcag ccgcgcaatg attccggcat
      241 tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg acgtcaatca
      301 ggtcattggt gatactaccg cggtgcgcct gaatgtgatg ggcgaaaaaa cccacgatgc
      361 cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg cttttggcct
      421 tggtacagca aatcgtttgt atcttaatta tctgcatgtc actcagcaca acacgccaga
      481 cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg gagcggcggc
      541 cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt ccgattacga
      601 cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg ataacaccac
      661 cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga cggcgattat
      721 gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga cctagtcacg
      781 cacggcgaat accaaagatg tgagtaataa
//
LOCUS       DAJTYL010000321          785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYL010000321 DAJTYL010000000
VERSION     DAJTYL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.330"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>785
                     /locus_tag="OO572_004174"
     CDS             <1..>785
                     /locus_tag="OO572_004174"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6662935.1"
                     /translation="LWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGK
                     PTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPT
                     FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPR
                     VKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQV
                     IAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          167 a          218 c          225 g          175 t
ORIGIN      
        1 ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac cattggttct
       61 tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
      121 gctgcaggca aaccaacgga taaaccaaac ctagtgtgcg gtccggtaca aatctgctgg
      181 cataaattcg cccgctactg ggatgtggag ctgcgtgaga tccctatgcg ccccggtcag
      241 ttgtttatgg acccgaaacg catgattgaa gcctgtgacg aaaacaccat cggcgtggtg
      301 ccgactttcg gcgtgaccta caccggtaac tatgagttcc cacaaccgct gcacgatgcg
      361 ctggataaat tccaggccga caccggtatc gacatcgaca tgcacatcga cgctgccagc
      421 ggtggcttcc tggcaccgtt cgtcgccccg gatatcgtct gggacttccg cctgccgcgt
      481 gtgaaatcga tcagtgcttc aggccataaa ttcggtctgg ctccgctggg ctgcggctgg
      541 gttatctggc gtgacgaaga agcgctgccg caggaactgg tgttcaacgt tgactacctg
      601 ggtggtcaaa ttggtacttt tgccatcaac ttctcccgcc cggcgggtca ggtaattgca
      661 cagtactatg aattcctgcg cctcggtcgt gaaggctata ccaaagtaca gaacgcctct
      721 taccaggttg ccgcttatct ggcggatgaa atcgccaaac aggggccgta tgagttcatc
      781 tgtac
//
LOCUS       DAJTYL010000322          774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYL010000322 DAJTYL010000000
VERSION     DAJTYL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.331"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..160
                     /locus_tag="OO572_004175"
     CDS             <1..160
                     /locus_tag="OO572_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6662936.1"
                     /translation="DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEED
                     FSSAWTVR"
     gene            388..>774
                     /locus_tag="OO572_004176"
     CDS             388..>774
                     /locus_tag="OO572_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662937.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          237 a          118 c          165 g          254 t
ORIGIN      
        1 tgatattgtc agggatcttt cacgtctggg ctggacgaat gaacgtattg gtacagagct
       61 gggaatggac caggatgaag tgctgaggct gaaacagatt agcgggctga cagagttgtt
      121 tcaggaagag gatttcagct cggcttggac ggttcgttga tgttgttaaa tatgggcggt
      181 atttctgaaa gttgatactt tgggtagaaa aaatgatacc gaaaactatg gaggatgtgc
      241 ttgtcaggtg actggacacc ataggtctta ggctttgata acgggataag atgagcggct
      301 tttctcatac catcaggtgt gatgatggcg cagatttaca aaatctgatt gattctaaag
      361 atgtataagt aaaaatcaag ctttgatatg aaaaaacaga tatttattaa taataaacct
      421 cctgtagtcc cttactcagg gacgcacgcg aaaatattta aatacattga aatccctctg
      481 ccgtttttct actttatcta tacatcaggc gaaccattcc acatatctgt ccaaaatact
      541 gttatttatg tatcaaaata caatggtata tttattaata aattagttcc tttttctctt
      601 ttatttgaca gggatatatc tgttcttcaa agaagagata tttgtgttgt gcgatttacg
      661 tcagaggaaa taagtgaaca taacgtttta tttgatcatg acattgaacg attaaaaaaa
      721 atttccaaag ctcagcttat atctcccgat tatgttttga ttgatttttc tagt
//
LOCUS       DAJTYL010000323          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYL010000323 DAJTYL010000000
VERSION     DAJTYL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.332"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>764)
                     /locus_tag="OO572_004177"
     CDS             complement(<1..>764)
                     /locus_tag="OO572_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662938.1"
                     /translation="ADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNN
                     KPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSH
                     RSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDI
                     SVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKS
                     AKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQ"
BASE COUNT          209 a          182 c          138 g          235 t
ORIGIN      
        1 cctgttgatc aaaaagattt tctatttctc cactatcggc gaggcgtttt tcaaggagtg
       61 tttgcaacat cgagttgcca aggacaatgg atatcgtgcg atctttcgca gatttacacc
      121 ccatcacaac ggcaacattc gtatccccct caagagtttc atttatcgcc gtcgctaaaa
      181 tggtggtcaa acacgcaaac ggaactgtat ttctttcgtg atttaattta tctaccagtt
      241 cattccattt atcaaaagcc gggctatatt tgttgcgcac agaaatatcc gactggttaa
      301 acaattcagc catgcgattt tttatcatct cgatatccgg cgaaccagaa agactttgcg
      361 cataatacgg tcctgatgat gttgcataca ttaatgccaa gcccacacct gtcgccatga
      421 tgggcgcagc cacggttttg gcaagggcga cgctgcgatg gctatgcgct tcacaggcta
      481 aaggaacata ttccactgac tgcggtaatt ccttacatgc ctccctcatc attgcggtat
      541 gctttacaaa caacggtttt tcactgccca aaaaaacttt ctgcttaatt gcactaagaa
      601 aacgcaaatc aaaaataatt gtttttggtt tattattttt tggcacattt cctattatcc
      661 cgctaagaac cttccttgct gcatggagat ttttttgcac agcacataac acaattgatg
      721 aagccttctc cactgcatta ttgattttaa ttttaaaatc cgca
//
LOCUS       DAJTYL010000324          758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYL010000324 DAJTYL010000000
VERSION     DAJTYL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.333"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..573)
                     /gene="dkgB"
                     /locus_tag="OO572_004178"
     CDS             complement(<1..573)
                     /gene="dkgB"
                     /locus_tag="OO572_004178"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6662939.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEV"
BASE COUNT          205 a          165 c          174 g          214 t
ORIGIN      
        1 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
       61 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      121 gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca atgggatcgt
      181 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      241 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      301 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      361 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      421 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      481 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      541 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      601 ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa acgcagagga
      661 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      721 aggcttctta ataagtggcg gaacggacgg gactcgaa
//
LOCUS       DAJTYL010000325          758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYL010000325 DAJTYL010000000
VERSION     DAJTYL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.334"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..291
                     /locus_tag="OO572_004179"
     CDS             <1..291
                     /locus_tag="OO572_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6662940.1"
                     /translation="FPNQKLVAALKNCAAHNDLPLQFSTMKTGATDGGRYNVMGGGRP
                     VVALCLPTRYLHANSGMISKADYDALLTLIRGFLTTLTAEKVNAFSQFRQVD"
     gene            291..>758
                     /locus_tag="OO572_004180"
     CDS             291..>758
                     /locus_tag="OO572_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6662941.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFL"
BASE COUNT          176 a          205 c          198 g          179 t
ORIGIN      
        1 ttccccaacc agaaactggt agcagcgtta aaaaactgtg ccgcacataa cgatttaccg
       61 ctgcaatttt ccaccatgaa aaccggagcg acggatggcg ggcgctacaa cgtgatgggc
      121 ggcgggcgtc cggttgttgc gctgtgcctg ccaacccgct atctgcacgc taacagcggc
      181 atgatttcaa aagccgatta cgatgctctg ctcacgttga tacggggttt tctgacgacc
      241 ttaaccgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta atgctaaacg
      301 aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct ggtgagctgg
      361 cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat ctcaacttta
      421 cccttaacgg caaagcccgc attttcgcca gtggcagtgc gggctatcag ctggaaatct
      481 tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat cggctgctgg
      541 cgctgttatt actgaatact ttcactcccc gtgcgcaact cgcctcggcg cttaatttgc
      601 cagaaacgtg ggtagcagag cgtctgcccc agttaaaaca gcgttatgaa cgcacttgtt
      661 gcctggccag ccgccctggt ttgggccatt tcattgatga gacagaagag aaactcgtta
      721 tcttgctggc gaacttgctg cgcaaagatc cgttttta
//
LOCUS       DAJTYL010000326          752 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYL010000326 DAJTYL010000000
VERSION     DAJTYL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.335"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..283)
                     /locus_tag="OO572_004181"
     CDS             complement(<1..283)
                     /locus_tag="OO572_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HCR6662942.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNED"
     gene            complement(378..689)
                     /locus_tag="OO572_004182"
     CDS             complement(378..689)
                     /locus_tag="OO572_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6662943.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
BASE COUNT          177 a          190 c          168 g          217 t
ORIGIN      
        1 ggtcttcgtt ttgttcccca ccacgccaga ctggcccgac ggtgtgtact acggctttag
       61 cgggaagatc gcctgcaagc gtaataacgg catgccccgt agggcaatcg ccctgctgtt
      121 gcctgacttt taaacaggca tccagcaggg ccggacccgc tgcgcgatga atggccccat
      181 cgacgccgcc gcctcccatt aatgacggat tagccgcatt cacaatcaca tcaacggcca
      241 gtttggtaat atcaccctgc acaacatgaa tacgcgtttt cataacgcct ccttacacct
      301 tcttgtttct taagtgtaca gcaggctttt tggttaaatc gcatttttct gctcagcaac
      361 gtgagttgtt cagaaactta gttatattcg atgttcatta cagccagctt tttctgttta
      421 tcaagatact gcacttttac tgaggctatt tgcgcgtttt tgacattgcc gctcattaac
      481 gctttgctgg agtaggtttt gcccggaata gaaccattct gtgggcagtt catttctacg
      541 ggtgatgact gggtgctgac gtcacatgcc ggttccacaa tttggccata aaaatggatc
      601 acgccccctt gcgcggtgct tcctgcaatg gcaataaaag gaaaaaacaa actgccgagc
      661 ataagagagt ctaaaaaagg tctgaacatt tattctattt cctcaatgat tagtcatcct
      721 tgaaggttgt gttatccata ctttcaggtt gg
//
LOCUS       DAJTYL010000327          739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYL010000327 DAJTYL010000000
VERSION     DAJTYL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.336"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            167..406
                     /gene="relB"
                     /locus_tag="OO572_004183"
     CDS             167..406
                     /gene="relB"
                     /locus_tag="OO572_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6662944.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            406..693
                     /gene="relE"
                     /locus_tag="OO572_004184"
     CDS             406..693
                     /gene="relE"
                     /locus_tag="OO572_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6662945.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          197 a          157 c          190 g          195 t
ORIGIN      
        1 gctgacctgc acaatacagg atggacttag caatggctgc tcctggcaca aagcggacag
       61 tgatcaccgt tcttacgact actttctgac ttccttcgtg acttgcccta agcatgttgt
      121 agtgcgatac ttgtaatgac atttgtaatt acaagaggtg taagacatgg gtagcattaa
      181 cctgcgtatt gacgatgaac ttaaagcgcg ttcttacgcc gcgcttgaaa aaatgggtgt
      241 aactccttct gaagcgcttc gtctcatgct cgagtatatc gctgacaatg aacgcttgcc
      301 gttcaaacag acactcctga gtgatgaaga tgctgaactt gtggagatag tgaaagaacg
      361 gcttcgtaat cctaagccag tacgtgtgac gctggatgaa ctctgatggc gtattttctg
      421 gattttgacg agcgggcact aaaggaatgg cgaaagctgg gctcgacggt acgtgaacag
      481 ttgaaaaaga agctggttga agtacttgag tcaccccgga ttgaagcaaa caagctccgt
      541 ggtatgcctg attgttacaa gattaagctc cggtcttcag gctatcgcct tgtataccag
      601 gttatagacg agaaagttgt cgttttcgtg atttctgttg ggaaaagaga acgctcggaa
      661 gtatatagcg aggcggtcaa acgcattctc tgaaccaaag catgacatct ctgtttcgca
      721 ccgaaggtga cacttctgc
//
LOCUS       DAJTYL010000328          739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYL010000328 DAJTYL010000000
VERSION     DAJTYL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.337"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>739
                     /locus_tag="OO572_004185"
     CDS             <1..>739
                     /locus_tag="OO572_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6662946.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAH"
BASE COUNT          168 a          191 c          235 g          145 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacatta
//
LOCUS       DAJTYL010000329          736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYL010000329 DAJTYL010000000
VERSION     DAJTYL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.338"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>736
                     /locus_tag="OO572_004186"
     CDS             <1..>736
                     /locus_tag="OO572_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6662947.1"
                     /translation="KVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSG
                     SGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTI
                     NDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADAL
                     AADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNAS
                     VTVKEQNEPVAQVSQWKRYDSRTPVNL"
BASE COUNT          211 a          180 c          175 g          170 t
ORIGIN      
        1 aaggttattc tggcaaaatc cgcgggtgaa gagcaaaagc tacaaattat tgccgtatcg
       61 aataaaggcg atttaagttt ccctgcgcag caaaaatcac ttatcgcgct ggcaaataaa
      121 gcagttagcg gaagcggcgt tggcgaactc tcctcttcca gcctgaaacg ctggagtgcg
      181 gaaaactcgg taaccatgag cagtaaagtc agtggcatga atacgttgct ctccgttagc
      241 gcgcggacta ataaccctga acctggtttc cagttgatta accagcgaat cacccacagc
      301 acgattaacg acaatatttg ggcatcgcta caaaatgctc aaattcaggc gttgaaaacg
      361 ctcgaccagc gtccagcgga gaaattcgcc cagctgatgt atgagacgcg ctatgctgat
      421 gaccgcacga aattactgca agaaaatcag attgcacagt ttactgccgc agatgcgctg
      481 gctgccgatc gccagttgtt ttcatctcca gcggatatca cgtttgtcat tgtcggtaat
      541 gtcgcagaag acaaactcgt ggcgttaatt acgcgttact taggctcaat caaacactct
      601 gattcgccat tagccgcagg taaaccatta actcgcgcga cggacaacgc atcggttact
      661 gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt ggaagcgtta tgattcccgg
      721 acacctgtta atctgg
//
LOCUS       DAJTYL010000330          730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYL010000330 DAJTYL010000000
VERSION     DAJTYL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.339"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>730
                     /locus_tag="OO572_004187"
     CDS             <1..>730
                     /locus_tag="OO572_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR6662948.1"
                     /translation="NETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETG
                     LTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNI
                     GLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISR
                     QMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDT
                     TLELPLDSATTESLRAATHDVLAGL"
BASE COUNT          200 a          192 c          195 g          143 t
ORIGIN      
        1 aacgaaacca gcgatacctg gttcaacgcg gcaattgcga tgaacaagcc gtggtcggaa
       61 aaactgcacg atgtctctga agaagtgcac cgcgccctgc aaaaactgca gcagattgaa
      121 gaagaaaccg gcctgaccat cgagcaggtt aaagatatca accgtcgtat gtccatcggt
      181 gaagcgaaag cccgccgtgc gaagaaagag atggttgaag cgaacttacg tctggttatt
      241 tctatcgcca agaaatacac caaccgtggc ttgcagttcc ttgacctgat tcaggaaggt
      301 aacatcggcc tgatgaaagc ggttgataaa ttcgaatacc gccgtggtta caagttctcc
      361 acctacgcaa cctggtggat ccgtcaggcg atcacccgtt ctatcgcgga tcaggcgcgc
      421 accatccgta ttccggtgca tatgattgag actatcaaca aactcaaccg tatttctcgc
      481 cagatgctgc aagagatggg ccgtgaaccg acgccggaag aactggctga acgtatgctg
      541 atgccggaag acaagatccg caaagtgctg aagatcgcca aagagccaat ctccatggaa
      601 acgccgatcg gtgatgatga agattcgcat ctgggggatt tcatcgagga taccaccctc
      661 gagctgccgc tggattctgc gaccaccgaa agcctgcgtg cggcaacgca cgacgtgctg
      721 gctggcctga
//
LOCUS       DAJTYL010000331          729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYL010000331 DAJTYL010000000
VERSION     DAJTYL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.340"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            35..>729
                     /gene="curA"
                     /locus_tag="OO572_004188"
     CDS             35..>729
                     /gene="curA"
                     /locus_tag="OO572_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6662949.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVF"
BASE COUNT          164 a          156 c          241 g          168 t
ORIGIN      
        1 tgatggattc atgccaaaag cacaggagag cattatgggg caacaaaagc agcgtaatcg
       61 acgttgggtt ctggcctcgc gtccacatgg cgcaccggtt ccggagaatt tccgtcttga
      121 agaagatgat gtcgccacac cgggtgaagg acaggtgtta ctgcgcacag tttatttgtc
      181 cctggacccg tatatgcgtg gacgtatgag cgatgagcca tcttattcac cgccggttga
      241 tattggcggc gtgatggtcg gcggtacggt gagccgtgtc gtggagtcga atcatcctga
      301 ttatcagcct ggcgactggg tgctgggcta cagtggatgg caggactatg acatatccag
      361 tggtgatgac ctggtgaaac ttggcgatca tccgcaaaat ccatcgtggt cgctgggtgt
      421 gctggggatg ccaggcttta ccgcttatat ggggctgctg gatatcggtc agcctaaaga
      481 gggcgaaacg ttggtggtag ctgcggcgac agaaccagtg ggggcgacgg tggggcaaat
      541 cggcaaactt aaaggttgca gagtggtggg ggtagccggt ggcgcggaaa aatgccgcca
      601 tgctaccgag gtgttaggct tcgatgtttg ccttgatcac cacgcggacg attttgccga
      661 acaactggcg aaagcgtgcc caaaaggtat tgatatctat tatgaaaacg tgggcggtaa
      721 ggtatttga
//
LOCUS       DAJTYL010000332          718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYL010000332 DAJTYL010000000
VERSION     DAJTYL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.341"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..492
                     /gene="virK"
                     /locus_tag="OO572_004189"
     CDS             <1..492
                     /gene="virK"
                     /locus_tag="OO572_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6662950.1"
                     /translation="VIARLTFSVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYG
                     IFPKRIIFEAFCALMKACNISECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVA
                     GKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQ
                     NLN"
     gene            557..>718
                     /locus_tag="OO572_004190"
     CDS             557..>718
                     /locus_tag="OO572_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6662951.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAG"
BASE COUNT          202 a          138 c          157 g          221 t
ORIGIN      
        1 gttattgccc ggttaacatt ttcagtcatt ctgactcaaa atggacacat tgcttttatt
       61 ggcggattac agggagcccc taaaaatacc ggacctgatg ttattcggtg cgcaacccgg
      121 gcctgttatg ggattttccc aaaacggatt atttttgaag cattttgtgc gttgatgaaa
      181 gcttgcaata tctctgagtg tctggcagtc agtgagcaca gtcatgtttt ccggcaattg
      241 agatactggt atcagaaacg caaaaccttt gttgcggtct atagtgattt ctgggagtct
      301 gtagcaggaa aaacctgtgg tgactggtac agattaccaa cccaggtgat tcgaaagccg
      361 ctaagcgata tagccagtaa aaaacgctct gggtaccgaa aacgatacgc tttgctggat
      421 tatatccatg aaactactat ccgctctttg gatgcatatc ctgtacactc agaacatcag
      481 aatttaaatt aaggtttgat gtattctctg aataaaacag taatgataat tttggtagag
      541 gcattcgcac taaataatga aaaaatataa atctgagttt attcctgaat ttaagaaaaa
      601 ttacctttcc cctgtttact ggtctacatg gttccttttg ggaatgattg caggtatttc
      661 aatgtttccc ccttcattca gagatcctgt cttggcaaaa atagggcgtt gggcgggc
//
LOCUS       DAJTYL010000333          714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYL010000333 DAJTYL010000000
VERSION     DAJTYL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.342"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>714
                     /locus_tag="OO572_004191"
     CDS             <1..>714
                     /locus_tag="OO572_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCR6662952.1"
                     /translation="STVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASG
                     FAWQFSVLSRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAAR
                     LRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTE
                     LFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYR
                     QDCAHVDIISPGAFVKIGP"
BASE COUNT          152 a          185 c          215 g          162 t
ORIGIN      
        1 cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca
       61 tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc
      121 atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat
      181 ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc
      241 tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg
      301 cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc
      361 cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg
      421 cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg
      481 agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc
      541 atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag
      601 gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg
      661 attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg ccga
//
LOCUS       DAJTYL010000334          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYL010000334 DAJTYL010000000
VERSION     DAJTYL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.343"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..673
                     /locus_tag="OO572_004192"
     rRNA            <1..673
                     /locus_tag="OO572_004192"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAJTYL010000335          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYL010000335 DAJTYL010000000
VERSION     DAJTYL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.344"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            5..>693
                     /locus_tag="OO572_004193"
     CDS             5..>693
                     /locus_tag="OO572_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6662953.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          173 a          162 c          216 g          142 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggaggcgt tctggagcgt atccgcagac tgaccccacc
       61 acatgtaacc gccccattca gaacggttgc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt tgcgaggaga tcaaccgcct gaatcgtcag ttgcgggtgg aaaaaatcct
      181 gaaccgctcc ggcatccagc cgttgcaccg gaagtgttcg tttgcgaatt accgggcgca
      241 gaacgacggt cagcgacatg cactgagtca ggcgaaatcc atagctgacg aactgatgac
      301 aggctgtacg aattttgtgt tcagcggtaa gcccggcacc ggaaaaaatc atcttgcagc
      361 cgcgattggc aaccggctga tggcgaaggg gcgtagcgtg attatcgtca ccgtgtccga
      421 tgtcatgagc gtgttgcatg agagctacga caacggcaaa tccggtgaaa aatttttaca
      481 ggagctttgt ggtgttgacc tgctggtcct ggatgaaatt ggcatgcagc gggatacgaa
      541 aaacgagcag gtggtactga accagattgt tgatcgccgg acggcatcgt tacgcggtgt
      601 ggggatgctg acaaatatta accatgcagc gatgaataca cttctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTYL010000336          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYL010000336 DAJTYL010000000
VERSION     DAJTYL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.345"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            5..693
                     /locus_tag="OO572_004194"
                     /pseudo
     CDS             5..693
                     /locus_tag="OO572_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          175 a          164 c          218 g          136 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggcggcgt tctggaacgc atccgccgac tgaccccgca
       61 acatgtaacc gccccattca gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct
      181 gaatcgctca ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt accaggtgca
      241 gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg aactgatgac
      301 cgggtgtaca aattttgcgt tcagcggaaa acctgatacc gggtagaatc acttagcggc
      361 agctatcggg aatcgcctgc tgaaagacgg tcagacagtg attgtggtta ccgtggctga
      421 tgttatgagc gccctgcacg ccagctatga cgacgggcag tcaggcgaaa aatttttgcg
      481 ggagctgtgc gaagtggatc tgctggttct tgatgaaatt ggcattcagc gcgagacgaa
      541 aaacgagcag gtggtgctgc accagattgt tgatcgccgg acagcgtcga tgcgcagcgt
      601 ggggatgctg acaaacctga actatgaggt gatgaaaaca ttgctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTYL010000337          687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYL010000337 DAJTYL010000000
VERSION     DAJTYL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.346"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..560
                     /locus_tag="OO572_004195"
     CDS             <1..560
                     /locus_tag="OO572_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISEc37 family transposase"
                     /protein_id="HCR6662954.1"
                     /translation="AAEGSSKREWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYL
                     KKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVR
                     YYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFT
                     GLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          173 a          142 c          223 g          149 t
ORIGIN      
        1 tggcagcaga aggaagcagt aaacgggaat ggaatcgctt cctggacacg cattaccgcc
       61 gtggctggaa tgtcaacgta tcccgggtaa tggataatgc cacccatgtg gcggtgtact
      121 ttggctctta cctgaaaaag ccaccggtgc cgatgagtcg tctggagcat tatgccggtc
      181 aggatgaaat cggtctgcgt tacaacagtc accgaacaaa acgggaagaa tacctgttga
      241 tgagtggaga tgagttcatg gaaaggttct cctggcatgt ggcggataag gggttccgta
      301 tggtgaggta ctacggtttt ttgagtccgg cgaaacggcg gttactggaa gaagtggtgt
      361 acatcataac ggagacagtg agaaaaacgg cgatgcaaat cacctggaga gggatgtatc
      421 agaggttact gaaggttgac ccgctgaagt gcgtgctgtg cgggagtcag atgcgtttta
      481 cggggctgaa gcggggctac cgtctggcag agcaggtcct gatgcatgag ccgctggccc
      541 ggatgcggtg gtgcggctga gagccgcaga ggggaagttg cgtccatttt gagggaaacg
      601 gagcaaaaaa ccgccattca taccctgaat caatcagagc catccagttt aatcgtcggt
      661 tccgttcata tggagcaaaa gtggtgt
//
LOCUS       DAJTYL010000338          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYL010000338 DAJTYL010000000
VERSION     DAJTYL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.347"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>668
                     /locus_tag="OO572_004196"
     CDS             <1..>668
                     /locus_tag="OO572_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR6662955.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTYL010000339          665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYL010000339 DAJTYL010000000
VERSION     DAJTYL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.348"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..212
                     /locus_tag="OO572_004197"
     CDS             <1..212
                     /locus_tag="OO572_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6662956.1"
                     /translation="LTAYAAQPMSNLDSGQLRPAGTVSATGASNLSDLEDKLAEKARE
                     QGAKGYVINSAGGNDQMLGTATIYK"
     gene            477..>665
                     /locus_tag="OO572_004198"
     CDS             477..>665
                     /locus_tag="OO572_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662957.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPK"
BASE COUNT          196 a          167 c          145 g          157 t
ORIGIN      
        1 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
       61 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
      121 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
      181 agatgttagg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
      241 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc aatgtaaata
      301 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc atcaagtgat aatgcttatc
      361 aaaattatta tcacttgcac gaacactatc acgggattaa cagtggcatc gcatccgcag
      421 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
      481 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
      541 gtattggtat tacgcctgtg gcacaggcac tcgccgccga agggcaagct aacgcggatg
      601 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
      661 cgaaa
//
LOCUS       DAJTYL010000340          662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYL010000340 DAJTYL010000000
VERSION     DAJTYL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.349"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(13..174)
                     /locus_tag="OO572_004199"
     CDS             complement(13..174)
                     /locus_tag="OO572_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662958.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(171..359)
                     /locus_tag="OO572_004200"
     CDS             complement(171..359)
                     /locus_tag="OO572_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6662959.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
BASE COUNT          207 a          160 c          116 g          179 t
ORIGIN      
        1 ctggctcact ttttatgatg atgccggtgc atttatctcc agcaccagac tttctatctc
       61 aacgccatac gttgcatttt tggtaatatc cgtcagcgtc agtgcattta gtcccactgc
      121 cagactgtct tttatggcct ggaatgccgg gccagtacga tgacgtagta tcactccggc
      181 tcagttgcac cactgaccac cacatcacct tctgctgcaa tcgcctgcat cagggtataa
      241 ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt atgtgtcagc
      301 ttttccgcaa gatctgacgg cggcgccgcc ctgacaacat catagtgttt aaatgccatg
      361 gttctttcca acatctgaaa aataattctt taaaatacct gacatgtaat acagaaaaaa
      421 cacaaaacca taccttaaat aaaaacctga ttatcaagca gatatgcatg gataaactac
      481 aagacgagat ataaaccacc ctgcatttaa ataaacaata aacaacatca gaaaaataat
      541 tctgttctat ggtttaattc aaaaatatca tttatacttt tcagaacatc accagcaagg
      601 cataaacaag gaaactaaat gaagtggatt gtgattgata cagttatcca gccatcatgc
      661 gg
//
LOCUS       DAJTYL010000341          662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYL010000341 DAJTYL010000000
VERSION     DAJTYL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.350"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            78..>662
                     /locus_tag="OO572_004201"
     CDS             78..>662
                     /locus_tag="OO572_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6662960.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          139 a          178 c          198 g          147 t
ORIGIN      
        1 ggtatcagcg ccggcggtat gtactgcccg gagtgtggca cggttcactg gccggacgga
       61 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
      121 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      181 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      241 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      301 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      361 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      421 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      481 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      541 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      601 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      661 gg
//
LOCUS       DAJTYL010000342          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYL010000342 DAJTYL010000000
VERSION     DAJTYL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.351"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..212
                     /locus_tag="OO572_004202"
     CDS             <1..212
                     /locus_tag="OO572_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662961.1"
                     /translation="VFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMSHAQIRNDMS
                     NKRKAEARQKAERAENIKKNDKNPA"
     gene            286..360
                     /locus_tag="OO572_004203"
     tRNA            286..360
                     /locus_tag="OO572_004203"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Arg,seq:cct)
     gene            522..>656
                     /locus_tag="OO572_004204"
     CDS             522..>656
                     /locus_tag="OO572_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6662962.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     G"
BASE COUNT          186 a          167 c          148 g          155 t
ORIGIN      
        1 tggtgtttaa cggtacgctg cactggagcg atttcatctg gcccttcgca ctacctactt
       61 tagcggggaa catctgcggc ggcaccttta tcttcgcgtt aatgagtcat gcacagattc
      121 gtaacgacat gagcaacaag cgtaaagcag aagcacgcca aaaagcagaa cgtgcggaaa
      181 acattaagaa aaatgataaa aacccagcat aaatggcgag ggtttaagca atcgagcggc
      241 agcgtactta ccccgcagtc cattagcggg tatactcgcc gcattgtcct cttagttaaa
      301 tggatataac gagcccctcc taagggctaa ttgcaggttc gattcctgca ggggacacca
      361 tttatcagtt cgctcccatc cgtaccagtc cgcaaaatcc cctgaatatc aagccttccg
      421 tatattcaca gttcgtcatg gttcgcgtca gatcgttgac agccgcactc catgacgggt
      481 aaaaagtgga taaaataatt ttacccaccg gatttttacc catgctcacc gttaagcaga
      541 ttgaagcagc aaagccgaaa gaaaaaccat accgccttct cgatggtaat ggcctgtacc
      601 tttatgtccc tgtatccggg aaaaaggtat ggcagcttcg ctacaagatt gacggt
//
LOCUS       DAJTYL010000343          650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYL010000343 DAJTYL010000000
VERSION     DAJTYL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.352"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            18..>650
                     /locus_tag="OO572_004205"
     CDS             18..>650
                     /locus_tag="OO572_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6662963.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKG"
BASE COUNT          140 a          197 c          167 g          146 t
ORIGIN      
        1 tgtttccgga tttttttatg cacattggac aggctcttga tctggtatcc cgttacgatt
       61 ctctgcgtaa cccactgact tctctgggag attacctcga ccccgaactc atctctcgtt
      121 gccttgccga atcaggtact gtaacgctac gcaagcgccg tcttcccctc gaaatgatgg
      181 tctggtgtat tgttggcatg gcgcttgagc gtaaagaacc tcttcaccag attgtgaatc
      241 gcctggacat catgctgccg ggcaatcgcc ccttcgttgc ccccagtgcc gttattcagg
      301 cccgccagcg cctgggaagt gaggctgtcc gccgcgtgtt cacgaaaaca gcgcagctct
      361 ggcataacgc cacgccgcat ccgcactggt gcggcctgac cctgctggcc atcgatggtg
      421 tgttctggcg cacaccggat acaccagaga acgatgcagc cttcccccgc cagacacatg
      481 ccgggaaccc ggcgctctac ccgcaggtca aaatggtctg ccagatggaa ctgaccagcc
      541 atctgctgac ggctgcagcc ttcggcacga tgaagaacag cgaaaatgag cttgctgagc
      601 aacttataga acaaaccggc gataacactc tgacgttaat ggataaaggt
//
LOCUS       DAJTYL010000344          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYL010000344 DAJTYL010000000
VERSION     DAJTYL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.353"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            36..>648
                     /gene="rlmB"
                     /locus_tag="OO572_004206"
     CDS             36..>648
                     /gene="rlmB"
                     /locus_tag="OO572_004206"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6662964.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRR"
BASE COUNT          154 a          174 c          191 g          129 t
ORIGIN      
        1 gtgcatcagg caaaacgtaa acaacgagta cattaatgag cgaaatgatt tacggcatcc
       61 acgcagtgca ggccctgctg gagcgcgccc ctgaacgttt tcaggaagtc tttattttaa
      121 aaggccgtga agataaacgt ctgttaccgc tgattcacgc ccttgagtct cagggcgtgg
      181 ttatccagtt ggcaaaccgc caatatctcg acgagaaaag cgacggtgcc gtgcatcagg
      241 gcattatcgc ccgcgtgaag ccaggacgtc agtatcagga aaacgatctg ccggatctga
      301 tcgcttcgct cgatcaaccg ttcctgctga tcctcgacgg cgtaaccgat ccgcacaacc
      361 tcggcgcgtg cctgcgtagt gcggacgccg caggcgttca tgcggtgatt gtgccgaaag
      421 atcgctccgc acagctcaac gctacggcga aaaaagtagc ctgcggcgcg gcagaaagcg
      481 ttccgctgat tcgggtgact aaccttgcgc gcaccatgcg tatgttgcag gaagagaata
      541 tctggatcgt cggtacggca ggcgaggcgg atcatacact ctatcagagc aaaatgaccg
      601 gacgcctggc gctggtgatg ggcgcggaag gtgaaggtat gcgtcgcc
//
LOCUS       DAJTYL010000345          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYL010000345 DAJTYL010000000
VERSION     DAJTYL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.354"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>637
                     /locus_tag="OO572_004207"
     CDS             <1..>637
                     /locus_tag="OO572_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6662965.1"
                     /translation="VSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDISNVWARDY
                     LDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIGGSYSVTVA
                     HNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAETNPTTYSDA
                     LERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASLFEV"
BASE COUNT          205 a          124 c          131 g          177 t
ORIGIN      
        1 gtttctgaat tagcgaaaag agtttctggt aaaacaaacc gaaaacttgt agcaacaatg
       61 ttgtctctgg ctgttgcagg tacagtaaat gcagcaaata ttgatatatc aaatgtatgg
      121 gcgagagact atcttgatct tgcacaaaat aaaggtattt tccagcccgg agcaacagac
      181 gtaacaatca ctttaaaaaa cggagataaa ttctctttcc ataatctctc aattccggat
      241 ttttctggtg cagcagcgag tggcgcagct accgcaatag gaggttctta tagtgttact
      301 gttgcacata acaaaaagaa ccctcaggcc gcagaaactc aggtttacgc tcagtcttct
      361 tacaaggttg ttgacagaag aaattccaat gattttgaga ttcagaggtt aaataaattt
      421 gttgtggaaa cagtaggtgc caccccggca gagaccaacc ctacaacata ttctgatgca
      481 ttagaacgct acggtatagt cacttctgac ggttcaaaaa aaatcatagg ttttcgtgct
      541 ggctctggag gaacatcatt tattaatggt gaatccaaaa tctcaacaaa ttcagcatat
      601 agccatgacc tgttaagtgc tagtctattt gaggtca
//
LOCUS       DAJTYL010000346          629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYL010000346 DAJTYL010000000
VERSION     DAJTYL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.355"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..629
                     /locus_tag="OO572_004208"
                     /pseudo
     CDS             1..629
                     /locus_tag="OO572_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          133 a          190 c          171 g          135 t
ORIGIN      
        1 caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg tcgacacaca
       61 gctgccggtg cggggagatt tttcagtgcc gacacaaggt gcggaatcgc cctggggcgg
      121 cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac aaacgccgca
      181 agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc gcccaccgga
      241 ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag atcccgccgc
      301 attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct tgtagcaata
      361 tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc cttcgcccgc
      421 gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct ttaaccccgc
      481 acatgcgcag caaaaaatcg ccagcggcga tttgccggca agcagttaca gttttggctt
      541 tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg cgaatgccag
      601 tgctgcggcg aaggtagttg ccaatttcc
//
LOCUS       DAJTYL010000347          629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYL010000347 DAJTYL010000000
VERSION     DAJTYL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.356"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>629
                     /locus_tag="OO572_004209"
     CDS             <1..>629
                     /locus_tag="OO572_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6662966.1"
                     /translation="WSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLT
                     PVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDN
                     LFQYVSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNV
                     SRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQI"
BASE COUNT          167 a          131 c          146 g          185 t
ORIGIN      
        1 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
       61 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
      121 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
      181 aacaaaaaca atttgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
      241 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
      301 gataatctgt tccagtatgt cagttacgtc catgacttac ctggcggcat tcgtgtctcg
      361 ttatccattg atattcttta ctttatcacg tcttcgagga aaagcgttct gttctggatt
      421 ttgacggcgt taattttgct gaatatggtc cggatgcact tccgtttata ccaaaatgtg
      481 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
      541 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
      601 attgacatgg acaagttaaa gcaaataaa
//
LOCUS       DAJTYL010000348          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYL010000348 DAJTYL010000000
VERSION     DAJTYL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.357"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            18..491
                     /locus_tag="OO572_004210"
     CDS             18..491
                     /locus_tag="OO572_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6662967.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          207 a          111 c          125 g          183 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcaga
//
LOCUS       DAJTYL010000349          625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYL010000349 DAJTYL010000000
VERSION     DAJTYL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.358"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          226 a          102 c          116 g          181 t
ORIGIN      
        1 atcctgtttt tgaaccattt atttgtaatg cgataaaatt ggctattggg taataaaaac
       61 cataggttgg ataatttttc aatctttgat aattgatttc cgattatgaa aaataaaact
      121 gagaaggaga aaataattga gtaactaaaa gtactcaggc cataagtatg catgaaaatt
      181 gatggtgata ttgatatgta gcaaaataga gaactgagta ttaatgagac aagtatagtg
      241 ccgcagataa actctttggt cacaaaggat gcactgtttt gacatattac tttctcattt
      301 gcatgagtta tctttggtga taataacatt attataatac tgaacagcat cataacaaga
      361 aatataacat tccagttata atattcaata atcaatgcgc gaggtgctac taatggtatt
      421 actgcgctta cagatgatat atcagaaagc gcccgtttta gttttaatgg agggtgatct
      481 tacccaccaa tagtggacac aggactaagt gagtaaactc tcaaccagag gtgactcatg
      541 acaaaaccag tatcaatcag caagaagccc cgtaaacaac atacgcctga atttcgtaac
      601 gaagccctga aactcgctga acgca
//
LOCUS       DAJTYL010000350          622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYL010000350 DAJTYL010000000
VERSION     DAJTYL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.359"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            2..>622
                     /gene="dcuC"
                     /locus_tag="OO572_004211"
     CDS             2..>622
                     /gene="dcuC"
                     /locus_tag="OO572_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6662968.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIIS"
BASE COUNT          125 a          155 c          168 g          174 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cg
//
LOCUS       DAJTYL010000351          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYL010000351 DAJTYL010000000
VERSION     DAJTYL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.360"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>620)
                     /gene="pdeF"
                     /locus_tag="OO572_004212"
     CDS             complement(<1..>620)
                     /gene="pdeF"
                     /locus_tag="OO572_004212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6662969.1"
                     /translation="LSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGV
                     SYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQ
                     QALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGSFLPVAHEFGLSSSIDM
                     WVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAK"
BASE COUNT          163 a          161 c          148 g          148 t
ORIGIN      
        1 tttagccagc aactgactga cttcaacagg aaaacgagcc tgacataccg aggttggaga
       61 cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca taaattgcag
      121 cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg
      181 caagaagcta ccggggccga tcagttcatc attctcacct ttcatgcgca gaagaatttc
      241 atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa aatggttgtg
      301 ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt cgcgttgcaa
      361 atacattgcc ccgcaacgct gcatattttc cggggcgttg gtcacgatgg aaagttcggc
      421 gaccgtattt agctctccca gcagcaggta gatatgattc actggcgagc gcacatagca
      481 gtaactgacg ccaatctgcg gttgcatcgg catcccatcc caaaagaaac gaaattgctt
      541 gagatgacta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc
      601 gagatcgtta cccgaaagct
//
LOCUS       DAJTYL010000352          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYL010000352 DAJTYL010000000
VERSION     DAJTYL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.361"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..473
                     /locus_tag="OO572_004213"
     CDS             <1..473
                     /locus_tag="OO572_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6662970.1"
                     /translation="DVYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTT
                     HKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIK
                     VNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT          196 a          116 c          122 g          185 t
ORIGIN      
        1 ctgatgttta tattcggggc gatattacag ctccgcaaag ttgcgaaata aataatttaa
       61 agccagtttg ttttgatttt aaagaaatac cagctgcaga tttttcatct gttgttggaa
      121 gtgcggtaac aacacataaa attactaaaa cagtcactat tgagtgtgaa aacctgggga
      181 tactgaatac cgatgatatc agtacgtctt tttatgctac cgaacccaat acggacaatt
      241 caatggtcgt aacttcaaac tctaacgtgg ggataaaaat ttacgacaaa aataataagg
      301 aaatcaaagt gaacggtggt gagttgccaa cagacatgga taaatcaaca gtgtatggtg
      361 aaaaatcagg tagtgtaact ttttcagctg ctcctgcgag ccttactgga gctcgcccag
      421 ccccggggca atttactgca acagcgacga taactgttga gattgtgcgc taataggtct
      481 gacaacgatt gttcatcaca tcgttgtcat ctcacccatt tctttatcac ttttaaatgc
      541 atcatgacaa aaagatggca tgtggaattt tcatcttcac atttaaaatc agtaagttat
      601 ttggtaattc ctttctgtc
//
LOCUS       DAJTYL010000353          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYL010000353 DAJTYL010000000
VERSION     DAJTYL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.362"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..169
                     /locus_tag="OO572_004214"
     CDS             <1..169
                     /locus_tag="OO572_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6662971.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..>591
                     /locus_tag="OO572_004215"
     CDS             154..>591
                     /locus_tag="OO572_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6662972.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIR"
BASE COUNT          157 a          128 c          173 g          133 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg a
//
LOCUS       DAJTYL010000354          582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYL010000354 DAJTYL010000000
VERSION     DAJTYL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.363"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>582)
                     /locus_tag="OO572_004216"
     CDS             complement(<1..>582)
                     /locus_tag="OO572_004216"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6662973.1"
                     /translation="VVALRNAIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNP
                     PKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYV
                     IDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLA
                     RLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC"
BASE COUNT          162 a          116 c          161 g          143 t
ORIGIN      
        1 gcatccccaa cgaacgaatt catccacata acgtgtgtag ttggtaaaca agacgaacgg
       61 ttgaaaatgt tctactggcg taccggtata atggcgcaac cgtgcgaggg aaaaatcgac
      121 gcggcgcgca tcaaaatgcg atagcgggga gaattccgtt ggatgataaa tgccgtctgc
      181 agtttcatcg ccgatttgcg ccagttctgt tgtcgggaag tagcgagtta aatcagcgct
      241 cattgagcga tcaagtgtca attcagagcc atcgatgaca taaggataag ggatttcatg
      301 ttgcgagggt tgcacagaga tatgcgcacc ataatcctga taaagcaacg ttagttgctc
      361 attcagatac gaacgaaaga gagtagggcg agtaatcgtg gtggtgtagc tgcctgcgtg
      421 agtaaaacga ccaaatgcgc gcgttttggg gggatttgtt gtgctaccgt cccaggttac
      481 ggtcagtgat ggatagacaa aaagaccttg tttacgggcg ttttcatcag gtaattcgcc
      541 aattgtaata tatttgccaa tggcgttgcg taaagcaact ac
//
LOCUS       DAJTYL010000355          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYL010000355 DAJTYL010000000
VERSION     DAJTYL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.364"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            36..419
                     /locus_tag="OO572_004217"
     CDS             36..419
                     /locus_tag="OO572_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662974.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          137 a          155 c          164 g          122 t
ORIGIN      
        1 cctgcgtgtc gtctggcggc tgaggaacat atactatgta cgcaaaatct tttctcgcac
       61 ttgatggcaa cggacgtctg accggtgccc gtaccgcaca gactgcacct tacgatagct
      121 acacctgcca cctttgcggc agtgcactca gataccaccc gcaatacgac actgaacggc
      181 cctggtttga acacgctgac gaagggctga cggagcacgg tcatgagtgc ccttatgtca
      241 ggccggaacg cagagaaata cggttgatta aacgtctgca gcaattcgtc ccggatacct
      301 tacccgtagt acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg
      361 agcagtactg cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa
      421 cggtataaaa acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactctgc
      481 gctcagcaga aatatccggc agtgtttctg cctttcctgc catggccggt tgattctgca
      541 gacagatgct cagggggcgt ggtttgagca tgatttac
//
LOCUS       DAJTYL010000356          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYL010000356 DAJTYL010000000
VERSION     DAJTYL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.365"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>577)
                     /gene="ipaH4.5"
                     /locus_tag="OO572_004218"
     CDS             complement(<1..>577)
                     /gene="ipaH4.5"
                     /locus_tag="OO572_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6662975.1"
                     /translation="VEEQCTRDYHRIWDDWAREGTTTENRIQAVRLLKICLDTREPVL
                     NLSSLKLRSLPPLPLHIRELNISNNELISLPENSPLLTELHVNGNNLNILPTLPSQLI
                     KLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPELPSTLTILRIEGNRLTVLPELPH
                     RLQELFVSGNRLQELPEFPQSLKYLKVGENQL"
BASE COUNT          165 a           97 c          136 g          179 t
ORIGIN      
        1 agttgatttt cacctacctt caaatatttt aagctctgag gaaattctgg tagttcctgt
       61 agtctgttgc cggaaacaaa gagttcttgt agtctatgag gcaattcagg caagacagta
      121 aggcgattac cttcaatacg taatattgtt agcgttgatg gaagctctgg taacgtctcc
      181 agactattaa aacgtgccga gagtgattgt aaatatggtg gtaatgatgg cagacatgac
      241 aaatttcgat tgaatgaaat attaagctta atcagttgag atggaagtgt cgggagtata
      301 ttcaagttgt taccatttac atgaagttct gtcaaaagcg gagaattttc aggtagggag
      361 attaactcat tgttggaaat attaagttca cgtatatgca aagggagtgg tggtaaagaa
      421 cgtagtttca gtgagcttaa attgagaaca ggctcccggg tatccagaca tattttcaat
      481 aatcgaaccg cctggatgcg attttctgtt gttgttcctt ccctagccca gtcatcccag
      541 atgcggtggt aatctctggt acactgttct tctaccg
//
LOCUS       DAJTYL010000357          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYL010000357 DAJTYL010000000
VERSION     DAJTYL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.366"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            10..>566
                     /locus_tag="OO572_004219"
     CDS             10..>566
                     /locus_tag="OO572_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6662976.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEP"
BASE COUNT          145 a          122 c          144 g          155 t
ORIGIN      
        1 atttactcga tgaatatatc agaacaacaa ctgagtaata tgatggtggc agtcactatc
       61 gcactgcagc cactggtcag ggtattgccg gtgacggctg tagaatgggc cgatcaaaat
      121 tattacctgc ctaaagaatc gtcatacggt gagggggaat ggaaaacgct gcagtttcag
      181 gtcgccatca tgaactgcat gggcaatgac gagattcgta cagttaacct gattaaatct
      241 gcccgtgttg gttatacaaa aatggtgctt ggtgtgatcg ggtattttat tgagcacaaa
      301 tcccgcaaca ctcttctttt tcagccgacg gactcagctg ctgaagattt tatgaagtct
      361 cacgtggaag caacaattcg tgatgttcct tgcctgaaaa aactttctcc ctggctgggc
      421 cggaaacacc gggataatac gctcacactg aagcgttttt cctctggtgt ggggttctgg
      481 tgtttgggtg gtgctgctgc caaaaactac cgtgaaaaat ctgtcgatgt ggtctgctat
      541 gacgaactct cctcgtttga accgga
//
LOCUS       DAJTYL010000358          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYL010000358 DAJTYL010000000
VERSION     DAJTYL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.367"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>565)
                     /locus_tag="OO572_004220"
     CDS             complement(<1..>565)
                     /locus_tag="OO572_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662977.1"
                     /translation="DGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGF
                     TKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSN
                     GSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTA
                     KNKFIPRVFFSANKSTRIGMLCGLLETD"
BASE COUNT          141 a          161 c          115 g          148 t
ORIGIN      
        1 atccgtttca agcagaccac acagcatgcc tatacgcgtt gatttatttg cggaaaaaaa
       61 cacacgaggg atgaatttgt tttttgctgt gcaccccatt agtccataac ctcgcagggt
      121 ctccatgagt ggatttacct gaccacgcgc cgttgatatc agccagtcct taccatctcc
      181 agagaagttg taatcaggca gttcagcctt catacgctcg atcatgtatg gctcgacgtt
      241 tgaaaacttc acgctcccat tgctaagact accatccccg agaagggagc cgataaccca
      301 tgctaatggc tctgagtggt cgccaaagtc accagttatt tcaggaatcc taacgccatt
      361 tttgtgcctc ttcgtttcac tcaaccgctt caacccgaag gtatctatca cgcggcgttt
      421 ctcgcctttc gtgaatccac gactagtgac ctcccatagg tgatcgcctg cgcaatcaac
      481 ataacgacca tcttcaaatg tgactcggta aacatctcta acaccctgcg ggaatatccc
      541 agtgacagtt gacggattgc cgtct
//
LOCUS       DAJTYL010000359          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYL010000359 DAJTYL010000000
VERSION     DAJTYL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.368"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>565
                     /locus_tag="OO572_004221"
     CDS             <1..>565
                     /locus_tag="OO572_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6662978.1"
                     /translation="FTQVWRRLLLGETVNFNGKHIHVRGAKLLFPPIQQPYPPLYFGG
                     SSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAYGRKIRFGIRLHVIVRETN
                     DEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGV
                     GLVRGGAGTALVGDGPTVAARINEYAAL"
BASE COUNT          129 a          151 c          167 g          118 t
ORIGIN      
        1 atttacccag gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca
       61 tattcatgtg cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct
      121 ttactttggc ggatcgtcag atgtcgccca ggagctggcg gcagaacaag ttgatctcta
      181 cctcacctgg ggcgaaccgc cggaacttgt taaagagaaa atcgaacaag tgcgggcgaa
      241 agctgccgcg tatgggcgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga
      301 aactaacgac gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac
      361 tatcgccaaa gcacaagccg cattcgcccg gacggattcc gtaggacaac agcgaatggc
      421 ggcgctacat aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt
      481 tggcttagtg cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgccgc
      541 gcgaatcaac gaatacgccg cgctt
//
LOCUS       DAJTYL010000360          562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYL010000360 DAJTYL010000000
VERSION     DAJTYL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.369"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(65..>562)
                     /locus_tag="OO572_004222"
     CDS             complement(65..>562)
                     /locus_tag="OO572_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6662979.1"
                     /translation="KPYDKDAEWARAGKVILPLLQNMLSDPYFSQPAPKSTGREYFNY
                     GWLERHLRHFPGVDPRDVQATLAELTAVTISEQVLLSGGCERLMVCGGGSRNPLLMAR
                     LAVLLLGTEVTTTDAVGISGDDMEALAFAWLAWRTLAGLPGNLPSVTGASQETVLGAI
                     FPANS"
BASE COUNT          140 a          156 c          142 g          124 t
ORIGIN      
        1 accctcctta atcttccaag tgtaacggca gacacgataa gaaaaaattc agttaattct
       61 gatatcacga gttggcgggg aaaatagccc ccagtaccgt ctcctggctt gcgccagtga
      121 cggaaggcag atttcctggt aatcccgcca gcgtccgcca ggcaagccag gcgaaagcca
      181 atgcttccat gtcatcgcca ctaatgccaa cggcatcggt ggtggtgact tctgtgccta
      241 gcagtaatac cgccagacgc gccatgagta gcggattacg actacctcca ccacatacca
      301 tcaatcgttc gcagccaccg ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt
      361 ctgccagtgt ggcctgaaca tcacggggat caacacccgg aaaatggcgt aaatggcgct
      421 ccagccagcc gtagttaaag tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat
      481 acgggtcgct gagcatattt tgcagcagtg ggagaataac tttacccgcc cgtgcccact
      541 cggcatcttt atcgtaaggt tt
//
LOCUS       DAJTYL010000361          560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYL010000361 DAJTYL010000000
VERSION     DAJTYL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.370"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..320)
                     /locus_tag="OO572_004223"
     CDS             complement(<1..320)
                     /locus_tag="OO572_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6662980.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTI"
BASE COUNT          166 a           98 c          124 g          172 t
ORIGIN      
        1 ttgattgtca gactattggc gttggtattg gtagaaatac cgtcgtccag atagtaggta
       61 gaaatcacgc cgttaacggt agcgttatta atgaccgggt agatatcgcc gttgtaatag
      121 ctatcagcgg catagttgtt ccagcctaca taaccatcat agtaaacgtc atcagcatag
      181 gtggcgtcgt tgtagtgatg gaaagtattg taagtttgac cagaaatatc gttggtcgct
      241 gcattagcct gagaggtgat agccagagtg caagccaatg ctaattgtga tactacaagt
      301 ttctttttcc aggagtgcat ttgtcatccc tcctcaggga cgtaataagt tgatccatca
      361 attatcaatg catagaaaac atattatgtt tcgcgctaac gattatgctg cgttacagac
      421 gaatggttca attattcttt cccctaagtc cgaatgtgga catatataat ttatggagaa
      481 aaacacgcat caaattgata aaaggacgat atttcagtat gttgcagata atgttatttt
      541 tgatgcatac gcgttataaa
//
LOCUS       DAJTYL010000362          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYL010000362 DAJTYL010000000
VERSION     DAJTYL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.371"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..353
                     /gene="istB"
                     /locus_tag="OO572_004224"
     CDS             <1..353
                     /gene="istB"
                     /locus_tag="OO572_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6662981.1"
                     /translation="QLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQEEAKLFF
                     QVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRLR
                     QKRKAGVIAEANPE"
BASE COUNT          148 a          141 c          147 g          116 t
ORIGIN      
        1 ttcagttatc tacggcacaa cgtcagggcc gttataaaac gacgcttcag cgtggagtaa
       61 tggccccccg cctgctcatc attgatgaaa taggctatct gccgttcagt caggaagaag
      121 caaaactgtt cttccaggtc atcgctaaac gttacgaaaa gagcgcaatg atcctgacat
      181 ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg tgatgcagca ctgacctcag
      241 cgatgctggg ccgtatctta caccactcac atgtcgttca aatcaaagga gaaagctatc
      301 gactcagaca gaaacgaaag gccggggtta tagcagaagc taatcctgag taaaacggtg
      361 gatcaatatt gggccgttgg tggagatata agtggatcac ttttcatccg tcgttgacat
      421 ctgtcagtaa tgaatcgctg ctacccgaca gcacggaagt aagctgccgg gcggggacgg
      481 aagtgtgagg tatttcaggg gcagacaccc gacatcccag aaaccgtcgg tcccgtccgg
      541 ggccggcacc ca
//
LOCUS       DAJTYL010000363          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYL010000363 DAJTYL010000000
VERSION     DAJTYL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.372"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>551
                     /locus_tag="OO572_004225"
     CDS             <1..>551
                     /locus_tag="OO572_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6662982.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMET"
BASE COUNT          163 a          134 c          147 g          107 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc g
//
LOCUS       DAJTYL010000364          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYL010000364 DAJTYL010000000
VERSION     DAJTYL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.373"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>548
                     /locus_tag="OO572_004226"
     CDS             <1..>548
                     /locus_tag="OO572_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6662983.1"
                     /translation="AQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISFI
                     GVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTSL
                     ADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGIF
                     ATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          138 a          145 c          134 g          131 t
ORIGIN      
        1 gcgcagaagt ttaagccgca ggcatacacc cttgataccg atggtaacaa agagaacgtc
       61 gacaaccgtg ttgcaacggt gaccgtatcc tccaccaccg gatttaagcg cggcgacaaa
      121 atcagcttca ttggtgtgaa gttcctgtct cagatggcga agaatgtgct gactgatgac
      181 gcgactttct caatcacccg tgtgatcgat ggtactcaca tcgaaatcac gccgaagccg
      241 attgcgctgg atgacgcgtc actgacaaaa gaagagaagg cttacgctaa cgtaaacacc
      301 tctcttgctg ataccactcc ggtaaacgtt ctgaacgtgg caacaaccac cgctaacgtg
      361 ttctgggctg atgactcaat ccgcctgctg tctcagccga tcccggtaac ccatgaactg
      421 tttgctggca tgaaaacgtc ttccttcagc attccaggta ttggtgttaa cggcatcttc
      481 gcaacgcagg gtgatatcaa cactctgtct ggtaagtgcc gtattgctgt gtggtattca
      541 gcatgtgc
//
LOCUS       DAJTYL010000365          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYL010000365 DAJTYL010000000
VERSION     DAJTYL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.374"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     misc_feature    complement(10..133)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(109..150)
                     /gene="hisL"
                     /locus_tag="OO572_004227"
     CDS             complement(109..150)
                     /gene="hisL"
                     /locus_tag="OO572_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6662984.1"
                     /translation="MTRVQFKHHHHPD"
     gene            433..>547
                     /locus_tag="OO572_004228"
     CDS             433..>547
                     /locus_tag="OO572_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCR6662985.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACV"
BASE COUNT          166 a          108 c          121 g          152 t
ORIGIN      
        1 aaccgcgcaa taaaaaagcc cccggaaggt gatcttccgg gggctttctc atgcgttcat
       61 gcaccactgg aagatctgaa tgtcttccag cacacatcgc ctgaaagact agtcaggatg
      121 atggtggtgt ttaaattgaa cgcgtgtcat aaaaatctct gtgaatgttt attcaactga
      181 tgtcttttaa cctaaaccac tttcacgtta gaaagcaagg gcttttttat atattttatg
      241 gcgatggatt tgtttaatga atgagcgggg aaaggagggg gctaagagga ctgccctcta
      301 acttgacagc accaaaaaga gcaaagtctg atttttcgtc tcgctggcaa tgtcttgatc
      361 tacaaactaa ttaataaata gttaattaac acctatcatt gtacaatgaa ctgtacaaaa
      421 taggagattt acatgcgtac aattagctac agcgaagcgc gtcagaattt gtcggcaaca
      481 atgatgaaag ccgttgaaga tcatgccccg atcctcatta ctcgtcagaa tggagaggct
      541 tgtgttc
//
LOCUS       DAJTYL010000366          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYL010000366 DAJTYL010000000
VERSION     DAJTYL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.375"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(37..545)
                     /locus_tag="OO572_004229"
                     /pseudo
     CDS             complement(37..545)
                     /locus_tag="OO572_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
BASE COUNT          154 a          166 c          129 g           96 t
ORIGIN      
        1 aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc cgtcaaagtt
       61 gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc
      121 gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac
      181 acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa tccccgccag
      241 tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc
      301 catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt
      361 acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact
      421 aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
      481 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
      541 cgcaa
//
LOCUS       DAJTYL010000367          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYL010000367 DAJTYL010000000
VERSION     DAJTYL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.376"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            16..531
                     /locus_tag="OO572_004230"
                     /pseudo
     CDS             16..531
                     /locus_tag="OO572_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          110 a          144 c          152 g          125 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
      241 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
      421 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
      481 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa t
//
LOCUS       DAJTYL010000368          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYL010000368 DAJTYL010000000
VERSION     DAJTYL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.377"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>528
                     /gene="tagK"
                     /locus_tag="OO572_004231"
     CDS             <1..>528
                     /gene="tagK"
                     /locus_tag="OO572_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HCR6662986.1"
                     /translation="NLSTVKTISQTAITSEAITEYLDLDWFRQQHLTSQNPFDIIPVH
                     GASSTGEGAEKGNALTRLYQEYREALLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLV
                     TEAETLQDMVTGGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNT
                     VLPDLTRKEHRIIGI"
BASE COUNT          145 a          141 c          120 g          122 t
ORIGIN      
        1 gaacctgagc acagtaaaaa cgatatcgca gactgccatc acgtcggaag caataaccga
       61 atatctggat ctggattggt tcaggcagca gcatctgaca tcacaaaatc cattcgatat
      121 cattcctgtt catggagcct cctccaccgg agaaggtgcg gaaaaaggaa atgccctgac
      181 ccgactttat caggaatacc gcgaggcgtt gttatctccc gaacaagatc ttaatcgcca
      241 tgctgatcct tttcctttta acgaagagcc ctcctcgcag gatctggatt cattgcgtga
      301 cctggtcacc gaagccgaga cattgcagga tatggtcact ggtgggttaa gcattgacgc
      361 tattctcaat gtactggatg ccactggcga agatgaaact atctggcctg tcgaaaagac
      421 tccccctgat atcctgcatt tactttcccc ggaatacgcc ccggaaacag cgcataacac
      481 cgtactgcca gatctgacac ggaaagagca tcgcatcatt ggcattga
//
LOCUS       DAJTYL010000369          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYL010000369 DAJTYL010000000
VERSION     DAJTYL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.378"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            158..424
                     /locus_tag="OO572_004232"
     CDS             158..424
                     /locus_tag="OO572_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6662987.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
BASE COUNT          124 a          115 c          152 g          137 t
ORIGIN      
        1 tcagagtaat gaatgttttg atcaaacaga gggcaaaaaa atagccataa aaaagtaaaa
       61 atgcggggca acgcgcagga cagctctcga cgccgccctc tgtgctgcct attatttatg
      121 gcggtgtcgt ttggcttgaa gatcagagga agaagagatg cgagcagcgt tttgggtagg
      181 gtgtgccgct ttattgttgt cggcgtgtag tagtgagcct gttcagcagg cgactgcggc
      241 gcacgtagcg ccaagtttaa aagcgtcgat gtccagtagt ggagaagcaa attgtgcaat
      301 gatcggcggt tcgctttctg ttgcccgtca actggatggt acggcgattg ggatgtgtgc
      361 attacccaac ggcaaacgct gtagcgaaca gtcacttgcc gccgggagct gtggcagcta
      421 ttaattcatt aaatccgcca gcttataagt taatgtctgt ttcgcggtcg ccagcgttaa
      481 ctggttcgcg gtcagatcca cttgtgcacc ttctttcagc atttcgct
//
LOCUS       DAJTYL010000370          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYL010000370 DAJTYL010000000
VERSION     DAJTYL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.379"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            21..446
                     /gene="arsC"
                     /locus_tag="OO572_004233"
     CDS             21..446
                     /gene="arsC"
                     /locus_tag="OO572_004233"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6662988.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
BASE COUNT          134 a          130 c          140 g          122 t
ORIGIN      
        1 ctttgtaatg agatactgat atgagcaaca ttaccattta tcacaacccg gcctgcggca
       61 cgtcgcgtaa tacgctggag atgatccgca acagcggcac agaaccgacc attatccatt
      121 atctggaaac tccgccaacg cgcgatgaac tggtcaaact cattgccgat atggggattt
      181 ccgtacgcgc gctgctgcgt aaaaacgtcg aaccgtatga ggagctgggc cttgcggaag
      241 ataaatttac tgacgatcgg ttaatcgact ttatgcttca gcacccgatt ctgattaatc
      301 gcccgattgt ggtgacgccg ctgggaactc gcctgtgccg cccttcagaa gtggtgctgg
      361 aaattctgcc agatgcgcaa aaaggcgcat tctccaagga agatggcgag aaagtggttg
      421 atgaagcggg taagcgcctg aaataaagcg gcgatatcca ctgccgatag ttggtaaagt
      481 gcgctttgtt tatgtcggat gcgacgctaa cgcgtcttat ccgacc
//
LOCUS       DAJTYL010000371          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYL010000371 DAJTYL010000000
VERSION     DAJTYL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.380"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..184
                     /locus_tag="OO572_004234"
     CDS             <1..184
                     /locus_tag="OO572_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6662989.1"
                     /translation="PVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNMETA
                     HQLELLSNLICRALRK"
     gene            356..>522
                     /locus_tag="OO572_004235"
     CDS             356..>522
                     /locus_tag="OO572_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6662990.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPG"
BASE COUNT          155 a          110 c          128 g          129 t
ORIGIN      
        1 gccggttttt gccaatacgg aaaaattgaa cgatgctgtg gaagagctgc gtcagttgct
       61 cgataaccac catgacctga aagtcgtgga aacaccaatc tatggttatg tgtggctgaa
      121 catggaaacg gcacatcagc ttgagttgct atcgaatctg atttgccggg ccttgcgcaa
      181 ataattcctg aacttcagaa tcatcttgct gctgcttcga ttcagcaagg ataaagggta
      241 tgatagtgaa aagggataaa agcattgtca tctgcggcag ctatgagtaa tgttggccct
      301 aacgaatagc ggttgcttaa acgaatccga ctctcacatt atcaggggta taaaaatgga
      361 aactaccaag ccttcattcc aggacgtact ggaatttgtt cgtctgttcc gtcgtaagaa
      421 caaactgcaa cgtgaaattc aggacgttga gaaaaagatc cgtgacaacc agaagcgcgt
      481 cctgttgctg gacaacctga gcgattacat caagccaggg at
//
LOCUS       DAJTYL010000372          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYL010000372 DAJTYL010000000
VERSION     DAJTYL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.381"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(259..>520)
                     /locus_tag="OO572_004236"
     CDS             complement(259..>520)
                     /locus_tag="OO572_004236"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6662991.1"
                     /translation="FRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQAGRP
                     YNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT          153 a          121 c          104 g          142 t
ORIGIN      
        1 gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc ttcatcatat
       61 ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc acaagtcaac
      121 gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgct gagaagggta
      181 tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct gctcaccctg
      241 tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc
      301 gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt cattaccacc
      361 aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac gcagcaccat
      421 gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc
      481 cagtaagtat tggctgccaa taaataattc acctcgaaaa
//
LOCUS       DAJTYL010000373          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYL010000373 DAJTYL010000000
VERSION     DAJTYL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.382"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          137 a          119 c          133 g          127 t
ORIGIN      
        1 actgacgagc ttgccggggc acctttgttt gccggtcatt ctggtatcat gcagatattt
       61 acggcgtaca agcccactag catgacattg taataaggcc tgagcctaac gctttaatgt
      121 gtgtcataca ctcagtggcg taatcgttga caatatggtg catgtaatcg taagcgtcca
      181 gcgaaatcca tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg
      241 atctgcgccc aatatcctga gccggaactt tgacaaaggc caacgaaaaa tgggagcgga
      301 tgttacagag ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt
      361 taaccgggaa attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata
      421 cgatgctata cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatatgga
      481 tcagggttgg caatatcgaa tcgcaggcta taggca
//
LOCUS       DAJTYL010000374          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYL010000374 DAJTYL010000000
VERSION     DAJTYL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.383"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>511)
                     /locus_tag="OO572_004237"
     CDS             complement(<1..>511)
                     /locus_tag="OO572_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR6662992.1"
                     /translation="SDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFG
                     GVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALIS
                     PVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTGFFLW
                     HHWPQMMRLM"
BASE COUNT          128 a          147 c          130 g          106 t
ORIGIN      
        1 ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa
       61 ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc gagaatatcc
      121 gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa
      181 ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg ccgctaatca
      241 gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat
      301 tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc
      361 gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca
      421 gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt
      481 gaggtgtggg ggcttctgtt ttgtcgtcgc t
//
LOCUS       DAJTYL010000375          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYL010000375 DAJTYL010000000
VERSION     DAJTYL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.384"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..453)
                     /gene="bglJ"
                     /locus_tag="OO572_004238"
     CDS             complement(<1..453)
                     /gene="bglJ"
                     /locus_tag="OO572_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6662993.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSR"
     gene            complement(411..>506)
                     /locus_tag="OO572_004239"
     CDS             complement(411..>506)
                     /locus_tag="OO572_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6662994.1"
                     /translation="AQGARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT          142 a          132 c          102 g          130 t
ORIGIN      
        1 ccggctttgg ttaatgtacc actgattgtt cagtcggtcg gtcgcctgac gtacaccatt
       61 taatgacaaa aagagttcct gatgaaaaat ctccagcgtt gacgctttac ttaatacacc
      121 gtccagcggt gatggcgaca atgaaccaat cagccgagct tcaatatcat catccacaat
      181 aactaaacgc cgggtacgcg gaaacttaat cgccagctca gtcaggcaag ataatccctc
      241 tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc gccgcaaacg agatccgcga
      301 catggcatcc tgaaatgact cctgactggt ataggtatgg agcttataag ggttgcccgc
      361 aaactttctg aataaactct caataccgat actactcatg acgcattttt ctatgagtgc
      421 gacatttctc ttcttaattc ggctgtgttc cattctgcgg catctccata aacagaaggc
      481 aagttaagtt ctcttgcacc ctgcgc
//
LOCUS       DAJTYL010000376          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYL010000376 DAJTYL010000000
VERSION     DAJTYL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.385"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            155..>504
                     /locus_tag="OO572_004240"
     CDS             155..>504
                     /locus_tag="OO572_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6662995.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          126 a          139 c          127 g          112 t
ORIGIN      
        1 gggctgcatc cgactctttt atcaacgctt acgtaattaa gggacacagc ctaaatagct
       61 gcgcggaata gtagatccct ttgagggaac ttagcccgga ttgtgcgatc tgatcaatcg
      121 ccaaatcaaa acaaatcacc aaccggactg agcaatgccg atcatagcac caatttcccg
      181 tgacgaacga cgcctgatgc agaaagccat ccataaaaca cacgataaaa attatgcccg
      241 cagactgact gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgccagaac
      301 gctctgctgc gcccgttcct ctgttggacg ctggattaac tggttcacgc agtcgggtgt
      361 tgagggactg aaatcattac ctgccgggcg tgcccgtcgc tggccgtttg agcatatctg
      421 cacactgtta cgtgagctgg taaaacattc tcccggcgac tttggctacc agcgttcacg
      481 ctggagtaca gaactgctgg caat
//
LOCUS       DAJTYL010000377          502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYL010000377 DAJTYL010000000
VERSION     DAJTYL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.386"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..478
                     /locus_tag="OO572_004241"
     CDS             <1..478
                     /locus_tag="OO572_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6662996.1"
                     /translation="ANTSISATQQPAIQQPNVSGTVWIRQKVALPPDAVLTVTLSDAS
                     LADAPSKVLAQKAVRTEGKQSPFSFVLPFNPADVQPNARILLSAAITVNDKLVFITDT
                     VQPVINQGGTKADLTLVPVQQTAVPVQASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT          117 a          150 c          123 g          112 t
ORIGIN      
        1 tgcaaatacg tctatttcag caacacaaca acctgctatc cagcaaccga atgtctccgg
       61 tactgtctgg atccgtcaga aagtcgcact gccgcctgat gctgtgctga ccgtgacact
      121 ttctgacgcg tcgttagccg atgcaccgtc aaaagtgctg gcgcagaaag cggtgcgtac
      181 tgaaggtaaa cagtcaccat tcagctttgt tctgccattt aacccggcag atgttcagcc
      241 gaacgcgcgt attctgttga gtgcggcgat taccgtgaat gacaaactgg tatttatcac
      301 cgataccgtt cagccggtga tcaaccaggg cggaactaaa gccgacctga cattggtgcc
      361 ggtacagcaa accgccgtgc cggttcaggc cagcggtggc gcaacgacta ccgtaccttc
      421 gacttcacca actcaggtga atccgtcttc ggcagttccc gctcctacgc aatattaagc
      481 gacgttaacc ctctccgccc gg
//
LOCUS       DAJTYL010000378          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYL010000378 DAJTYL010000000
VERSION     DAJTYL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.387"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>500
                     /locus_tag="OO572_004242"
     CDS             <1..>500
                     /locus_tag="OO572_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6662997.1"
                     /translation="NPEVVKTIAKTGAALLTMGVAIGTLTRITKIMGSVMNMTPAKGL
                     IALLVGGAYLIIDNWETVGPVVKKVWQEVDQVVRAMGGWEQAVKTIATVSALYIGVKA
                     VASIRAATVAQNQWTTAAGKTGLKLKGLGKISLIGGLLELGMMAQEFEKEHPWLVKNF
                     VADALN"
BASE COUNT          146 a           89 c          153 g          112 t
ORIGIN      
        1 aaatcctgag gtcgtaaaaa ccatcgcgaa aactggtgcg gcattactga cgatgggcgt
       61 ggcgattggc acattgacac gcataacaaa aatcatgggt agcgtgatga atatgacgcc
      121 ggcaaaggga ttaattgcgc ttctcgtcgg tggtgcatat ctcataattg ataactggga
      181 aacggtcggt ccggttgtga aaaaagtctg gcaggaggtt gatcaggttg tcagagcaat
      241 ggggggatgg gaacaggcag taaaaacaat agcaacggtt tctgccctgt atattggtgt
      301 gaaggcggtg gcaagtattc gtgcggcaac agtcgcccag aatcaatgga caacggcggc
      361 aggaaaaaca gggctgaaat taaagggcct cggaaagatc agtctgattg gtggattgct
      421 tgaactgggc atgatggcgc aggagtttga aaaagaacat ccctggcttg ttaaaaattt
      481 tgtggctgat gcactgaaca
//
LOCUS       DAJTYL010000379          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYL010000379 DAJTYL010000000
VERSION     DAJTYL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.388"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>494)
                     /locus_tag="OO572_004243"
     CDS             complement(<1..>494)
                     /locus_tag="OO572_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6662998.1"
                     /translation="FIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDTLLLFVICT
                     LMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAKRLGKRFL
                     MRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLMPGQAGSAT
                     TLYT"
BASE COUNT          142 a          140 c          121 g           91 t
ORIGIN      
        1 tggtatagag cgtggtggct gaacctgcct gaccgggcat caaatcctga aaatagagca
       61 tcccgatgcc gcccagaatg ccaataaaaa tagcatttag cagctgcaag cccaacagaa
      121 tggcaggtga atgcgccatc agcattcctg cgtaaaaaca gacgccaccc acggcagcaa
      181 cgcgcattaa gaaacgctta cccagacgtt tggcgaaata tccggcaatc aacatcgtcg
      241 ggatttccag cccggcggcg gtccccatca tcacaccggc cagtttctcg ggaagatgca
      301 gttcgttgat aataaatagc ggcatgttga tgatgtacag gctgttcgag ccccacatca
      361 atgtacaaat gacaaacagc agtagcgtat cgcgacggtt acggcgcggc gcttcaaccg
      421 tgccggtcgc cagcggaagc tctttttgca tcgacggtaa aaacagccac accatcacgc
      481 cgcaaacaat aaac
//
LOCUS       DAJTYL010000380          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYL010000380 DAJTYL010000000
VERSION     DAJTYL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.389"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..299)
                     /locus_tag="OO572_004244"
     CDS             complement(<1..299)
                     /locus_tag="OO572_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6662999.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFS"
BASE COUNT          154 a          100 c          104 g          131 t
ORIGIN      
        1 gagaaggtga taagagtgac tattggagga agtgagcatt tatcagctgc ccaaccaaca
       61 atcacactca atattcccat aaatcccacg tataacatgt agcgcatcgc tgtagtcaat
      121 gctttgaatg tgaatcttgc attcgcgaag aagctaaagc tcacagccac tacgaaacct
      181 gtgaagtttg ccagagcctg actggtatgt gcggcataga tacatacacc aaaaaccacc
      241 cagtgtataa gtgtgttcag cacacctata gatgtgtact ttacaaataa ctttaacatt
      301 tatttaatca atgagctctg aaaggcatga agtctatcat ccaagtctca attgatcgat
      361 acttgctatg tctgatgaga caaaactgag acacataagg cctcacaatg gcttgcaagg
      421 ctttacatgt tttgatgtgg tgggacgtgt gagcgcagtg ttgatgggat aatcctttga
      481 attacaagc
//
LOCUS       DAJTYL010000381          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYL010000381 DAJTYL010000000
VERSION     DAJTYL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.390"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..346
                     /locus_tag="OO572_004245"
     CDS             <1..346
                     /locus_tag="OO572_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6663000.1"
                     /translation="GIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAED
                     ERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYN
                     AIEWPASPEGSS"
     gene            370..>485
                     /locus_tag="OO572_004246"
     CDS             370..>485
                     /locus_tag="OO572_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6663001.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVA"
BASE COUNT          127 a          118 c          123 g          117 t
ORIGIN      
        1 aggcattgaa ccccgtaacg ccagtgttat tgaagttcct gatattaccg ccaaccgccg
       61 cattaccgcg ccgggttact ggttttaccg caatgatgaa tttgtctttg actacagact
      121 caaagcggaa gatgagcgtg atgcccttct ggctcaggtc agtgcccgga caggggaatg
      181 ggaagaagac ctgctgctgg ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg
      241 tcgtatttac gcgaaatcgc tgcaggcgat ggatttcagc accatcactg ataaatcctc
      301 atacaacgcc attgaatggc ccgcctctcc ggaaggttct tcctgattta atttatcgcg
      361 agaaaaacaa tgtctgtagt gatatcaggt gcgctgactg atggcgcagg tatccccatg
      421 tccggatacc atattattct gaaatcccgg gtaaaccccc cggaagtggt gatgaacact
      481 gttgc
//
LOCUS       DAJTYL010000382          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYL010000382 DAJTYL010000000
VERSION     DAJTYL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.391"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..153)
                     /gene="hokE"
                     /locus_tag="OO572_004247"
     CDS             complement(1..153)
                     /gene="hokE"
                     /locus_tag="OO572_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6663002.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT          131 a          131 c          103 g          120 t
ORIGIN      
        1 ctacttcttc ggttcgtaag caagaacagc cttaaactca atattacgtt cctttacggt
       61 gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac
      121 tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg
      181 gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata gaaatatctc
      241 ttggggcttc atctttccaa acctcagagt taacctgaaa ccggaaagct tcaggcaccc
      301 gccgttatct tacccttcct tagcaaaata gctgtttatt tatacagtga gtcctgtttc
      361 ggacttctgt cggcaaaact catcattgct cttaaaacga aatcgcccgc agccatcact
      421 gaccgcgggc gaaagtgtaa agcaggtgcc ttaccatcct gacctgacaa ccggatatgc
      481 gggga
//
LOCUS       DAJTYL010000383          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYL010000383 DAJTYL010000000
VERSION     DAJTYL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.392"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..203)
                     /locus_tag="OO572_004248"
     CDS             complement(<1..203)
                     /locus_tag="OO572_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6663003.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
BASE COUNT          124 a          124 c          118 g          116 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 ca
//
LOCUS       DAJTYL010000384          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYL010000384 DAJTYL010000000
VERSION     DAJTYL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.393"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>480
                     /locus_tag="OO572_004249"
     CDS             <1..>480
                     /locus_tag="OO572_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6663004.1"
                     /translation="KAFLGIKDDDHHFDARREKKFSRPDRKKIARYVTRTESHLEYLQ
                     SRGISPEVVKRYEVVSGKVWNGERELDALVLPYKRDGELLQVKRISTERPDGKKVIMA
                     EGDCEPCLFGWQALDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIE"
BASE COUNT          121 a          102 c          149 g          108 t
ORIGIN      
        1 gaaagcattt ctcggtatca aggatgacga tcaccatttc gatgccagac gtgagaagaa
       61 attctccaga cctgatcgta agaaaatcgc ccgctacgtt accagaacag aatcccatct
      121 tgagtacctg caatcgcgtg gcatatcgcc agaagtcgta aagcgctacg aggttgtcag
      181 cggcaaggtg tggaatggag aacgagaact ggatgctctg gtgcttccgt acaaacgcga
      241 tggtgagttg ttgcaggtca agcgaatcag cactgagcgc ccggacggga agaaagtcat
      301 tatggcagaa ggtgattgcg aaccttgcct gttcggatgg caggctctgg acgctggcgt
      361 gagggtggtt gtgctttgcg aaggcgaaat tgattgtatg agctatgcgc aatacggcat
      421 ctcggcgtta tccgtgccgt ttggtggcgg gaaaggcgct aagcaacagt ggattgagtt
//
LOCUS       DAJTYL010000385          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYL010000385 DAJTYL010000000
VERSION     DAJTYL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.394"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          167 a           74 c           70 g          164 t
ORIGIN      
        1 ccggaaagta tgcagaccat ctgccattat accgccaaag tgatctacta ttccacacag
       61 ctatttagct ctctttttgt tgagctccca tgattctttc atttattgtt actaattatt
      121 ttatgttatc atgatgttag aacacgctct gctctgtgtc tctattttat ctatcatatt
      181 ctgaggatta actacataac aaggcgcaag atatatatac aaaaacaaat aataccgttg
      241 caataattcc ttttagtaaa ggaggtgtga ttaatttcag gggatttcag agtaaaaatt
      301 gtacaacata ttaaaatatg ttcatttcgt tataatacac acaaggaaat attaagttag
      361 ataatatatt ctaaataacc atagataaaa acgcacataa tggggaatat tttatgaaaa
      421 tacctgaagc agtaaatcat attaatgttc agaataatat tgatcttgtt gatgg
//
LOCUS       DAJTYL010000386          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYL010000386 DAJTYL010000000
VERSION     DAJTYL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.395"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..347
                     /locus_tag="OO572_004250"
     CDS             <1..347
                     /locus_tag="OO572_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6663005.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          120 a          121 c          121 g          112 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg ccca
//
LOCUS       DAJTYL010000387          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYL010000387 DAJTYL010000000
VERSION     DAJTYL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.396"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..473
                     /locus_tag="OO572_004251"
                     /pseudo
     CDS             1..473
                     /locus_tag="OO572_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAJTYL010000388          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYL010000388 DAJTYL010000000
VERSION     DAJTYL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.397"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>472)
                     /locus_tag="OO572_004252"
     CDS             complement(<1..>472)
                     /locus_tag="OO572_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6663006.1"
                     /translation="IIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLGLRSIIAN
                     PEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGGEVLYADMGHFGKFSIRLAWFT
                     VVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIASQAV"
BASE COUNT          145 a          132 c          106 g           89 t
ORIGIN      
        1 taaccgcctg cgaggcaatt accgtcgcca gtgcggcgat gatcagcagt gggatcagcg
       61 cccagtccgg tgccaacagg aagaacgggt tcttaatcgc ttccgggttc tttaacaaca
      121 acgctccctg accgaagtaa ttaagggtca aggaaggtaa tacgacggtg aaccacgcca
      181 ggcgaataga gaacttacca aagtgcccca tatcagcata cagcacctcg ccccccgtaa
      241 tcgacagcac cactgcccct aatgcaataa aagaaaccgt tttgtattca aggaagaaat
      301 gcaccgccca catcggattc agcgcatgca gcacttccgg gttagcaata atgctacgta
      361 accccagtcc tgccagaatc aaaaaccagg tcagcatgat cggcgcaaac agcttaccga
      421 ccatagccgt gccatgcttt tgaatcataa ataataaagt gagaacgata at
//
LOCUS       DAJTYL010000389          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYL010000389 DAJTYL010000000
VERSION     DAJTYL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.398"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..361
                     /locus_tag="OO572_004253"
     CDS             <1..361
                     /locus_tag="OO572_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6663007.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAJTYL010000390          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYL010000390 DAJTYL010000000
VERSION     DAJTYL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.399"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>466)
                     /locus_tag="OO572_004254"
     CDS             complement(<1..>466)
                     /locus_tag="OO572_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6663008.1"
                     /translation="ESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDANSK
                     QMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVA
                     EYYEVEEKKEKVYIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKR"
BASE COUNT           95 a          126 c          113 g          132 t
ORIGIN      
        1 acgcttcacc ttgcgctctg ctaccttaat gaatccacga ttagccaggt cgtcgatgac
       61 gtctttgata tcctgttggt aatagctgac cggctcacct gtcagcgggt cgcggtagat
      121 gtatactttc tccttcttct cttcgacctc gtaatactca gcgacgtaga cgacatcatt
      181 cgacacccac ggaaacagcc atgtatcgtt cggattctgg aaagatggca aggtgtcagg
      241 atcaataccg taatcctctg cgaactcttt ccagccattg cgtgacaaag cgttaatcac
      301 cgtgcagtgc ttagcgtcgc tcttatccat ctgcttgctg ttggcgtccc atatgacgtg
      361 tgagcaggct tcatggattg gcaggcgtcg gattacctga ttgttgcttg ttggatcgtt
      421 gtcttcgtac tgggtgacca gacgccatgc accaacgccg gactct
//
LOCUS       DAJTYL010000391          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYL010000391 DAJTYL010000000
VERSION     DAJTYL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.400"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..205
                     /locus_tag="OO572_004255"
     CDS             <1..205
                     /locus_tag="OO572_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6663009.1"
                     /translation="PTGAVDALKEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKD
                     SAKTFVQETLKDTWESADEWFVH"
BASE COUNT          146 a          117 c          109 g           92 t
ORIGIN      
        1 gccaaccggt gctgtggatg cgttaaagga agaattaacc cgacgcatca gtaaacgtta
       61 tgacgatgta gaggtgatcg taaaagccac cagcaacgat ggcctttctg ttacgcgcac
      121 cgccgataaa gattcagcta aaacttttgt tcaggaaact ctgaaagata cctgggagtc
      181 tgctgacgag tggtttgttc actaattagc acgtaaaatc agtaacggct ggaaatcatt
      241 caatactcgc actatcggaa gttcaccagc cagccgcagc acgttcttgc atacgacgtg
      301 tctgcggtct caactccgcc cgcccccgcc aatcatgatt gggcagtacc agtacagcac
      361 tagcaaagac aggaaattag cagtctcagc aaaacaccga ccagacggtg agaagacaaa
      421 aagatacgca aagatgccgc ggttccagaa gaataaaaaa ccgc
//
LOCUS       DAJTYL010000392          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYL010000392 DAJTYL010000000
VERSION     DAJTYL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.401"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>463
                     /locus_tag="OO572_004256"
     CDS             <1..>463
                     /locus_tag="OO572_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6663010.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTYL010000393          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYL010000393 DAJTYL010000000
VERSION     DAJTYL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.402"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          116 a           97 c           88 g          155 t
ORIGIN      
        1 cactattcat atcttggtct attaatgcaa ataaaacgag aaagtcttta aaaaaacaat
       61 cctagagctt tatcggcgtt ttcattccgg ctggaggaat atgttcctgt ctttccagta
      121 accgtcgatt gttatatcag tctacccatg ttaatgtgac cagttctact ttcatccagt
      181 ttggattgat ctttatcccc tatattttca aacaattttt atcatttaac tcattactat
      241 ctcgatattc ccatagcaaa cgatctggtt ggttatcccc tcaaaaaagt cagttgcacg
      301 ccgtaaatcc ctgccggtat tgagcgagga tttacgggtt gttgttaagg tccgtaatcg
      361 ggtcacagtt tttccggagt caatccggtc tgcggtttat gcttatgcga cgtgattatg
      421 aatggtgcag tcgtgagccg ttttcagaca atcgca
//
LOCUS       DAJTYL010000394          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYL010000394 DAJTYL010000000
VERSION     DAJTYL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.403"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..262)
                     /locus_tag="OO572_004257"
     CDS             complement(<1..262)
                     /locus_tag="OO572_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6663011.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNND"
BASE COUNT          141 a           88 c           94 g          122 t
ORIGIN      
        1 catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc acagcttccc
       61 tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt cgcttacact
      121 gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc agagaaggaa
      181 ttgccttatt cataccaggg caaggtatta atttaaactg taataattta attttaggat
      241 gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg gcgttacagg
      301 aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca ataggtcaaa
      361 aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc gtcaaataat
      421 ccagacgtac caataagcgc tggta
//
LOCUS       DAJTYL010000395          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYL010000395 DAJTYL010000000
VERSION     DAJTYL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.404"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>444
                     /locus_tag="OO572_004258"
     CDS             <1..>444
                     /locus_tag="OO572_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6663012.1"
                     /translation="KYLTLALMGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDG
                     NNADICARGWNNAKAAFYADVPKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRV
                     IRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRFGSGKKESYSS"
BASE COUNT          110 a           98 c          115 g          121 t
ORIGIN      
        1 aaaaatactt aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta
       61 gcgatagtag cgatgtcgat aacgacggcg acggaacatt ttacgcgaca gtgcaggatt
      121 gtattgatga cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg
      181 cattttatgc cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa
      241 attgctacta cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa
      301 gccgggttat tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat
      361 ttgcttcccg ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg
      421 gcaaaaaaga gtcttactct tcag
//
LOCUS       DAJTYL010000396          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTYL010000396 DAJTYL010000000
VERSION     DAJTYL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.405"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..365
                     /gene="rplQ"
                     /locus_tag="OO572_004259"
     CDS             <1..365
                     /gene="rplQ"
                     /locus_tag="OO572_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6663013.1"
                     /translation="RQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPL
                     ITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAP
                     MAYIELVDRSEKAEAAAE"
BASE COUNT          106 a          116 c          113 g          105 t
ORIGIN      
        1 gtcgtcaact gaaccgcaac agcagccatc gccaggctat gttccgcaat atggcaggtt
       61 cactggttcg tcatgaaatc atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg
      121 tagttgagcc gctgattact cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat
      181 tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt
      241 tcgcgagccg tgccggtggt tacactcgta ttctgaagtg tggcttccgt gcaggcgaca
      301 acgcgccgat ggcttacatc gagctggttg atcgttcaga gaaagcagaa gctgctgcag
      361 agtaatctga agcaatgtaa aaaaacccgc cccggcgggt ttttttatac ccgtagtatc
      421 cccacttatc tacaatagct
//
LOCUS       DAJTYL010000397          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTYL010000397 DAJTYL010000000
VERSION     DAJTYL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.406"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          105 a           97 c          109 g          127 t
ORIGIN      
        1 cattgaataa ttttaacatt gatgcagtct ctttgagagc tgacatcgct aatacatcac
       61 ttgctcactc ttcttattgt tggggtattc agattataca aatttcttgt tgttggggaa
      121 taacaggaac cacctcgcct gacgtagtta aaagcaggca cacaacgcga aatgacgcgg
      181 cggtaagtat ggcggagtta ttccttcccc gttgaggaca ccgggttgtc aggttgacca
      241 tacgcttaag tgacaaccac gccacaacac tccatgttga ttggtacctt tggcggcatc
      301 agtttcattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      361 ggctgagcgc acgcggaaca gttaaaaagc gattagttcc cacgtatcgg gtggttatgg
      421 gtttccctgt atccggcg
//
LOCUS       DAJTYL010000398          436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTYL010000398 DAJTYL010000000
VERSION     DAJTYL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.407"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>436
                     /gene="aroD"
                     /locus_tag="OO572_004260"
     CDS             <1..>436
                     /gene="aroD"
                     /locus_tag="OO572_004260"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6663014.1"
                     /translation="STEAYIALNRAAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDV
                     KVVMSNHDFHKTPEAEEIIARLRKMQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQ
                     YADRPIITMSMAKTGVISRLVGEVFGSAATFGAVKKASAPGQ"
BASE COUNT          107 a          112 c          113 g          104 t
ORIGIN      
        1 tttccaccga ggcttatatt gctctcaatc gtgcagccat cgacagcggc ctggttgata
       61 tgatcgatct ggagttattt accggcgatg atcaggtcaa agaaaccgtc gcctacgccc
      121 acgcgcatga tgtgaaagtt gtcatgtcca accatgactt ccataaaacg ccggaagccg
      181 aagaaatcat tgcccgtctg cgcaaaatgc agtccttcga cgccgatatt cctaagattg
      241 cgctgatgcc gcaaagtacc agcgatgtgc tgacgttgct tgccgcgacc ctggagatgc
      301 aggagcagta tgccgatcgt ccaatcatca cgatgtcgat ggcaaaaact ggcgtaattt
      361 ctcgtctggt tggtgaagta tttggctcgg cggcaacttt tggtgcggta aaaaaagcct
      421 ctgcgccagg gcaaat
//
LOCUS       DAJTYL010000399          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTYL010000399 DAJTYL010000000
VERSION     DAJTYL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.408"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            2..>435
                     /gene="bglB"
                     /locus_tag="OO572_004261"
     CDS             2..>435
                     /gene="bglB"
                     /locus_tag="OO572_004261"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6663015.1"
                     /translation="MLYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNE
                     INMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIPEAKIGNMLLGGL
                     VYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR"
BASE COUNT           99 a          109 c          125 g          102 t
ORIGIN      
        1 aatgctatat gggctggtga aaaactacgg cggttgggct aatcgggcgg tcatcgatca
       61 cttcgagcat tacgcccgca cggtctttac ccgctaccaa cataaagtag cgttatggct
      121 gacgtttaat gaaatcaata tgtcgttaca cgcgccattc acgggcgtgg ggctggcaga
      181 agagagtggc gaggcggaag tttatcaggc gatccatcat caactggttg ccagtgcgcg
      241 ggcagttaaa gcctatcata gcctgatccc cgaagcgaaa atcggcaata tgcttctcgg
      301 tgggctggtt taccccctca cctgccagcc acaggatatg ttgcaggcca tggaagagaa
      361 ccggcgctgg atgttctttg gtgatgttca ggcgcgtggc caatatcccg gctatatgca
      421 gcgtttcttc cgcga
//
LOCUS       DAJTYL010000400          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTYL010000400 DAJTYL010000000
VERSION     DAJTYL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.409"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..269
                     /locus_tag="OO572_004262"
     CDS             <1..269
                     /locus_tag="OO572_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6663016.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>434
                     /gene="tnpB"
                     /locus_tag="OO572_004263"
     CDS             266..>434
                     /gene="tnpB"
                     /locus_tag="OO572_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6663017.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKI"
BASE COUNT          109 a          114 c          122 g           89 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atac
//
LOCUS       DAJTYL010000401          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTYL010000401 DAJTYL010000000
VERSION     DAJTYL010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.410"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            35..434
                     /locus_tag="OO572_004264"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO572_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTYL010000402          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTYL010000402 DAJTYL010000000
VERSION     DAJTYL010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.411"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..71
                     /locus_tag="OO572_004265"
     CDS             <1..71
                     /locus_tag="OO572_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HCR6663018.1"
                     /translation="LVARAKPGEYVQGINGKWVRKF"
     gene            complement(243..>433)
                     /locus_tag="OO572_004266"
     CDS             complement(243..>433)
                     /locus_tag="OO572_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6663019.1"
                     /translation="VVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFST
                     VFKQRFGMTPGEYRRKFR"
BASE COUNT          137 a           95 c           95 g          106 t
ORIGIN      
        1 agctcgtagc tcgggccaaa ccgggggaat atgtacaagg gataaacggt aaatgggtgc
       61 gaaagtttta atagcaaaac cgcaatatcg ggataacgct acaggagaaa gcgattgatc
      121 tattttcctg aaacaaggtg aatattcaaa aactcctgtc aaattgcctt tcgccctgaa
      181 aaatgcatag tcaaatatct gttttaacta attggcgttg cagtacatgc aacgccaatt
      241 agttagcgga atttacgtcg atactcgcct ggcgtcatcc caaagcgttg cttaaatacc
      301 gttgaaaaat gactctggtc agaaaatccc caatgaaagc cgatgcctgc cagtttttca
      361 tcatctgcgg catggcgaat cgcatctgca caaaaatcga gacgacggtt acgaatatat
      421 tgcgcgacta cca
//
LOCUS       DAJTYL010000403          432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTYL010000403 DAJTYL010000000
VERSION     DAJTYL010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.412"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..145
                     /locus_tag="OO572_004267"
     CDS             <1..145
                     /locus_tag="OO572_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6663020.1"
                     /translation="MLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQ
                     ELN"
BASE COUNT          121 a          108 c          103 g          100 t
ORIGIN      
        1 aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg ctaacgcggc
       61 gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca ccgtctccgg
      121 tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc gtatgtgttg
      181 tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg cagccaaatc
      241 acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt accttcgcac
      301 tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta ttcacgctaa
      361 ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac ggacccggat
      421 cctcaatgct gc
//
LOCUS       DAJTYL010000404          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTYL010000404 DAJTYL010000000
VERSION     DAJTYL010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.413"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          145 a           82 c           71 g          133 t
ORIGIN      
        1 tgttgttaat gacattaata acgtcgttct taaaatcctc aaagtcttgt tgcggtaact
       61 tctggttacg ctgcttacta tcagcaagcg gtgaatccgg acgacaacaa cataggtttt
      121 gtataacccc cagcattctt ttcacctccg ttaacagtat attcacgaca ctgaaacgag
      181 agaatgctga taattttctc ggcgagatat caaaaatcgc tcattataaa acaagtaact
      241 tcaacgtcgc gtaaccgtgg acgataaaaa aacaataatg tcattcatat ttaaataatt
      301 tagccttaaa tgatttaaat aatattagcc acctgataaa tacattttta atgatagaga
      361 gggtcaacag gcttgcggaa gtcgtcacat cttcatgttt tcaatagcta aactttgata
      421 ttgaacatga t
//
LOCUS       DAJTYL010000405          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTYL010000405 DAJTYL010000000
VERSION     DAJTYL010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.414"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..299
                     /locus_tag="OO572_004268"
                     /pseudo
     CDS             1..299
                     /locus_tag="OO572_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          141 a           82 c           93 g          114 t
ORIGIN      
        1 atctgccagc agtgcttcta ttattattca aaaagaactt cagccggaca atgctataat
       61 ccagggaaag gctataagct ctgttggacg gaatgtggaa atcagcactg gtaaaaaggg
      121 gaactgacat taacacaaga aggattcctg caagcaactg cttatctgcc cggaacgcag
      181 aattaaagca taaccacaca ggagccagaa tactgtggtc gtacgcattc agtattgtga
      241 caactaatag aaataaaaat ctggccggtg cgaatattaa acgatgcgat accgtatagt
      301 gtaagtcaaa agtaacgggg tatttaaacc atcttttcat atttatttaa cataatgcag
      361 attctgatgt attagttcag ttgttcgcca gaatcagcgc tcgatcacta tgcgttttag
      421 cagtgtgctg
//
LOCUS       DAJTYL010000406          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTYL010000406 DAJTYL010000000
VERSION     DAJTYL010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.415"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            104..>429
                     /locus_tag="OO572_004269"
     CDS             104..>429
                     /locus_tag="OO572_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6663021.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPD"
BASE COUNT          158 a           70 c           72 g          129 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgattt
//
LOCUS       DAJTYL010000407          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTYL010000407 DAJTYL010000000
VERSION     DAJTYL010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.416"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(5..220)
                     /locus_tag="OO572_004270"
     CDS             complement(5..220)
                     /locus_tag="OO572_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6663022.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          137 a          105 c           91 g           95 t
ORIGIN      
        1 gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag tacagatttg
       61 tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca gcccactgtg
      121 acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct gcggaggtgc
      181 cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct ccgcaaataa
      241 cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa aacgcaaaag
      301 cgggcgaaac gatatataca gtaaggaaag cactctatcc aacaaaccac ccacagttaa
      361 tcggaataaa agcagagtgc ttatgaatga tcgcacgccc gaaggttagt atttctgcac
      421 agcaattt
//
LOCUS       DAJTYL010000408          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTYL010000408 DAJTYL010000000
VERSION     DAJTYL010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.417"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>428)
                     /locus_tag="OO572_004271"
     CDS             complement(<1..>428)
                     /locus_tag="OO572_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6663023.1"
                     /translation="ALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKS
                     ISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQ
                     YYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          103 a          122 c          121 g           82 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgc
//
LOCUS       DAJTYL010000409          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTYL010000409 DAJTYL010000000
VERSION     DAJTYL010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.418"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..280
                     /locus_tag="OO572_004272"
     CDS             <1..280
                     /locus_tag="OO572_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663024.1"
                     /translation="VNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSW
                     RFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            283..>421
                     /locus_tag="OO572_004273"
     CDS             283..>421
                     /locus_tag="OO572_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6663025.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     AS"
BASE COUNT          131 a           84 c          106 g          100 t
ORIGIN      
        1 tgttaacgga caggtaattc cgttaaatta cggaaacttc gatgcccgct ataaatatcg
       61 aacagagggg gtacaggatg tacggtatgg ccatgaaatg tattacagcc ccggcagtaa
      121 caccgtttcg tggagatttt gcgcaccttc gggacacggg ctgtcaggga tggcgatatc
      181 ggataccggc cgtaactcag cggataacgt tgacggtgtg tattaccgac cactgcaaaa
      241 actgattaat ggcacctggt ataacgtagc gagtatttaa caatgttgca tttaaaaaat
      301 attactgcgg gtaatccgaa aacagcagaa caatatcaga tgacaaaacg atattcggtc
      361 acctggcttt tttcagaaga cggaaaaaac tggtatgaag agctgaagaa tttcgccagc
      421 g
//
LOCUS       DAJTYL010000410          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTYL010000410 DAJTYL010000000
VERSION     DAJTYL010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.419"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          137 a           72 c           97 g          110 t
ORIGIN      
        1 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
       61 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      121 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      181 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      241 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      301 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      361 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtga
//
LOCUS       DAJTYL010000411          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTYL010000411 DAJTYL010000000
VERSION     DAJTYL010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.420"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>411
                     /locus_tag="OO572_004274"
     CDS             <1..>411
                     /locus_tag="OO572_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6663026.1"
                     /translation="ASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQ
                     PDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITN
                     NGFDFKQTISTNLSIGETKFTGGITAHNSQIAIG"
BASE COUNT          150 a           75 c           73 g          113 t
ORIGIN      
        1 agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg ttttgaaaga
       61 aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata atcagcagac
      121 ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat tacacctgaa
      181 caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat cagcaaataa
      241 atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag ggaaaaatat
      301 tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca taggagaaac
      361 taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg a
//
LOCUS       DAJTYL010000412          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTYL010000412 DAJTYL010000000
VERSION     DAJTYL010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.421"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            42..401
                     /locus_tag="OO572_004275"
     CDS             42..401
                     /locus_tag="OO572_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663027.1"
                     /translation="MPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIEL
                     FRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQ
                     EGLVAPSQEPQDVKKAV"
BASE COUNT          147 a           78 c           80 g          105 t
ORIGIN      
        1 caacagggcg tccaatagag tggtttatcc aaagtcagag catgccaatg gttgagcata
       61 aaaataatca agagaataaa aattctcaac ctgaccgcag cacattgaaa actaaactga
      121 tcgcaattgt cgaattgtta gaggacaagg aactgcattc tgcgattgag ttgttcagaa
      181 agaaaggatt tgaagctctt atgcctgaga ttttttcttc cgatgattcc atccaggctg
      241 atttgcaagg tatttcgcag caaaccttac aaacagccaa aatgcttgaa tcattaccaa
      301 cagatactcg taaaaagatt ttgtcaaagt atggtattta tgaacaggaa ggtcttgtag
      361 ctccttcaca agaaccacaa gatgtaaaaa aagccgtata acaaccatga
//
LOCUS       DAJTYL010000413          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTYL010000413 DAJTYL010000000
VERSION     DAJTYL010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.422"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>408
                     /locus_tag="OO572_004276"
     CDS             <1..>408
                     /locus_tag="OO572_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6663028.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNY"
BASE COUNT          130 a           84 c           97 g           97 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatc
//
LOCUS       DAJTYL010000414          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTYL010000414 DAJTYL010000000
VERSION     DAJTYL010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.423"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..101)
                     /locus_tag="OO572_004277"
     CDS             complement(<1..101)
                     /locus_tag="OO572_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6663029.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYAL"
     gene            complement(236..>405)
                     /locus_tag="OO572_004278"
     CDS             complement(236..>405)
                     /locus_tag="OO572_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6663030.1"
                     /translation="EFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLV
                     FLVLLFLNQDK"
BASE COUNT          128 a           84 c           58 g          135 t
ORIGIN      
        1 atcaatgcat attcgattgc agtaacacca cgttcatcct tcgcgaaacg cgcaaagcac
       61 tgtttagttt taccatagta cttagtaata aaagtattca tgttttcctc gtatattatt
      121 atttaaattc aaaccaccag cttcatctgc gtgtcaccgg tatcccatat ttttgggatg
      181 tggctataat tatctattcc cgctatttag tcttcaaaaa aaattacatt taatatcatt
      241 tatcttgatt aagaaatagt aagactaaaa aaacaagcgt cgtcagccag cacactgcta
      301 acacggtaag aacgctaata aaaacactcc actgcttcgt tctataccat tcaatatttt
      361 tgtcagtatg ttttattggg agcagctctt cagtagagaa ttcct
//
LOCUS       DAJTYL010000415          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTYL010000415 DAJTYL010000000
VERSION     DAJTYL010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.424"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..249)
                     /locus_tag="OO572_004279"
     CDS             complement(<1..249)
                     /locus_tag="OO572_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663031.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPS"
BASE COUNT           89 a           95 c          127 g           93 t
ORIGIN      
        1 ggacggacgc ggtggccgtt cggaataagg gagcctgacg aagattatcc agtgacgata
       61 atcgagactg agggctttct taatctcgta tccgtgtctg cggtagcgct gaatcagcca
      121 ttcagcctgt tcttcggtgc aggggtcgtg ctgatacccg tcagatttga atgcatgaga
      181 acgccgcccg tgcctgctgg caggggcggc agagttatcc gaattgtaaa atttggtatc
      241 gtgcgccatc tgttttctct gctggcgcag caggtgccag ttgttcaggc tgacggatgg
      301 attgtaaacc agaacgacca gaaaaaacaa aacccgccga agcgggttaa gtgcgggtgc
      361 gttgaggatg cctgacacat cagcggtggc gagggatttc tccc
//
LOCUS       DAJTYL010000416          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTYL010000416 DAJTYL010000000
VERSION     DAJTYL010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.425"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            1..395
                     /locus_tag="OO572_004280"
                     /pseudo
     CDS             1..395
                     /locus_tag="OO572_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          104 a           95 c          106 g           90 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtcaggtc acggg
//
LOCUS       DAJTYL010000417          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTYL010000417 DAJTYL010000000
VERSION     DAJTYL010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.426"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>395)
                     /locus_tag="OO572_004281"
     CDS             complement(<1..>395)
                     /locus_tag="OO572_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6663032.1"
                     /translation="LNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLCV
                     KHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATIL
                     TLSHPDATIENGRAVTTSVAVAEFFRKMH"
BASE COUNT           98 a          103 c          106 g           88 t
ORIGIN      
        1 tgtgcatttt ccggaagaac tccgcaaccg caacagacgt agtgacagcg cgaccatttt
       61 cgatggttgc gtcagggtga gaaagggtaa ggatagtagc catgatggca gcctcgatag
      121 tcagtttaaa tgactcacca ccagaggtag caatctcatg ggtggtgaga cgcacagggt
      181 tgctacaacc ggtgactacc gaaaccggcc agccttgcgg ctgccctgca cgccccacca
      241 taatctgaat gtggctgtgc ttgacgcata aaaaaaccgc ctgagcgcgg ttatgcgcag
      301 tagtcatcgt cggggtagca atcccggcac ccgttttatg aggtgcaggt gcactataat
      361 tccacccgtt ctggttttca atagctacat tcaac
//
LOCUS       DAJTYL010000418          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTYL010000418 DAJTYL010000000
VERSION     DAJTYL010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.427"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..225)
                     /locus_tag="OO572_004282"
     CDS             complement(<1..225)
                     /locus_tag="OO572_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6663033.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGRDTARTLKIG"
BASE COUNT          113 a           88 c           96 g           97 t
ORIGIN      
        1 gccgattttc agtgtcctgg cggtatcacg gccccctgcg ccgttatgtg ccatttcaac
       61 gatctgctcc ttaacgccga gttttctggc ttcataagag taagtgagct ggaaaacacg
      121 tttacatgag cgacaacgga aacgctcatg ccgggaacaa ctacggccat gtcgataaac
      181 ttcatcggaa ttacagcgcg gacaatgaac agtaacggta gtcatggaaa acctcaaata
      241 ccagtagatt accagaaact tctatcgatt gaagacatta ccttttgccg caataggtgg
      301 ggtaacattg gtgaaaacgc attacgccgg gtggcagttg gaaaaaaatt atcttttttc
      361 ggctctgatc gtggtggaga aaatgcggca ataa
//
LOCUS       DAJTYL010000419          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTYL010000419 DAJTYL010000000
VERSION     DAJTYL010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.428"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>394
                     /locus_tag="OO572_004283"
     CDS             <1..>394
                     /locus_tag="OO572_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6663034.1"
                     /translation="PSEINRLKALVAKLQRMQFGKSSEKLRAKTERWIQEAQERISAL
                     QEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGELSPLG
                     CDVSEQLELISSAFKVIEKQRPKLACRR"
BASE COUNT           99 a          111 c          111 g           73 t
ORIGIN      
        1 ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt atgcagttcg
       61 gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa gcacaggagc
      121 gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat gacccggtac
      181 tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca cttccccgtg
      241 ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt gaactcagtc
      301 cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt aaggttatcg
      361 aaaaacaacg ccctaaactg gcctgtcgcc ggtg
//
LOCUS       DAJTYL010000420          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTYL010000420 DAJTYL010000000
VERSION     DAJTYL010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.429"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>391)
                     /locus_tag="OO572_004284"
     CDS             complement(<1..>391)
                     /locus_tag="OO572_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663035.1"
                     /translation="YAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRA
                     WALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAEL
                     LANDLTAVGFYRLAHVLAQFRNTESEAR"
BASE COUNT          102 a          109 c           97 g           83 t
ORIGIN      
        1 ccgtgcctcg ctttctgtat tacgaaattg tgccaacaca tgtgccagcc gataaaaacc
       61 caccgcggtg aggtcattcg ccagcaactc tgcctgatta atagcgctct gttcctgata
      121 gcgccagccg ttatggagca gttgaataag taacgcctgg cagcgcatca gcaactgatg
      181 agcagtagac ggcacaggca gaacgctggc agaaggtagt gataccacca caggcgcagt
      241 ttctgcgtcc agcgcccagg cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa
      301 ttgcccatca acaaaactga caaagccaga aaccagacac acggggtcgt ctgtttgttg
      361 caaaagcgcc gccatgcgtt caatggcata a
//
LOCUS       DAJTYL010000421          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTYL010000421 DAJTYL010000000
VERSION     DAJTYL010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.430"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          116 a           82 c           84 g          108 t
ORIGIN      
        1 tgagggagtt gtattcatta ccgttgtctc tcgtccaggt ttactcctga tagaaagtca
       61 tcggcacttg acggtaatta cttatgacca aaaacctctt tgcgtcacat ttttacaaca
      121 attattgcag agaaaattaa tgctattgga atgaagagag taacgaatgg ctaaactggc
      181 taacccgaat gacgaaaaat tctggaaaac agcacgcaat ttcgtgaagt gattattttc
      241 gtccctaagg aaacctcata ggatgcctgc gggtgcctga gactttctgt tttcaggcta
      301 accgggaatc agaaagacga aggccccgac cgatatttct atcaactgta ctgcacccat
      361 tttgttggac gatgaaatgg aatagcccct
//
LOCUS       DAJTYL010000422          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTYL010000422 DAJTYL010000000
VERSION     DAJTYL010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.431"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>389
                     /gene="tcyP"
                     /locus_tag="OO572_004285"
     CDS             <1..>389
                     /gene="tcyP"
                     /locus_tag="OO572_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6663036.1"
                     /translation="IESNYVGKVSDQSVPQLVLSFIPKNPFADLTGANPTSIISVVIF
                     AAFLGVAALKLLKDDAPKGERVLTAIDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAG
                     SNLQDIIKLGSFVVASYLGLLIMFAVH"
BASE COUNT           87 a           96 c           95 g          111 t
ORIGIN      
        1 ccattgaaag taactatgtt ggtaaagtct ctgatcagag cgttccgcag ctggtcttgt
       61 cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg tcaattatca
      121 gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg aaggatgatg
      181 cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct acaaagctgg gtgatgaaac
      241 tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg accaaagtgg
      301 ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc gcgtcctacc
      361 tcggtctgct gattatgttt gctgtgcat
//
LOCUS       DAJTYL010000423          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTYL010000423 DAJTYL010000000
VERSION     DAJTYL010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.432"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(139..>387)
                     /locus_tag="OO572_004286"
     CDS             complement(139..>387)
                     /locus_tag="OO572_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6663037.1"
                     /translation="HGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAV
                     AIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          105 a          109 c           99 g           74 t
ORIGIN      
        1 agacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg
       61 attttgacgc caccaatacc cgtgcggttt gtaatgtcga agataaaaca accagggcag
      121 ataaaatgcc aatgctaatt aatcacgctt ttccagcaga gtccggtaaa tcagaccacc
      181 gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta atgcccagcc
      241 gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg
      301 aatactgatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg caaaacctgc
      361 cggagcgaat ttgtcggttg caccgtg
//
LOCUS       DAJTYL010000424          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTYL010000424 DAJTYL010000000
VERSION     DAJTYL010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.433"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>387
                     /locus_tag="OO572_004287"
     CDS             <1..>387
                     /locus_tag="OO572_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6663038.1"
                     /translation="DAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVD
                     VAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASL
                     NTWPIACSDGQLHEVKYGLSSNSGET"
BASE COUNT          101 a           96 c           87 g          103 t
ORIGIN      
        1 tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg
       61 aacgctatcc ggaagtcact ttatcgctag tctcctccga aacgattatt aatttgatcg
      121 aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggactccagc ttacgtgcca
      181 ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg
      241 ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg
      301 cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg
      361 gtttgtcatc caatagtggg gaaacac
//
LOCUS       DAJTYL010000425          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTYL010000425 DAJTYL010000000
VERSION     DAJTYL010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.434"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>386
                     /locus_tag="OO572_004288"
     CDS             <1..>386
                     /locus_tag="OO572_004288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663039.1"
                     /translation="VALQAAGLDVCREYPVPERGDGCGGRIDIVVTDRNGVRCGIELD
                     RNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGILVIGGAVRQKKFDPLSVDLPDWL
                     SETLWHEWVQFRQALRKPIRTELGAN"
BASE COUNT           81 a           85 c          129 g           91 t
ORIGIN      
        1 tggttgcgtt gcaggctgct ggcctggatg tctgtcgtga gtatccggtg ccagagcgtg
       61 gtgacggttg cggagggcgg attgatatcg tcgtgactga caggaacggt gtccggtgtg
      121 ggatcgagct tgaccgaaat tctccgcgac agaaatcact gctcaaaatc ggtgctgttg
      181 aaaccgggat atgtgtcttg cggcgcagtg atatcgcaag gcacaccgag cagggaattc
      241 tggttatcgg tggggctgtt cgccagaaaa aatttgaccc gttgtcagtt gatctgcccg
      301 actggttgtc agaaacactc tggcatgagt gggtccagtt caggcaggca ttgcgaaaac
      361 cgattcgaac ggagctgggc gctaac
//
LOCUS       DAJTYL010000426          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTYL010000426 DAJTYL010000000
VERSION     DAJTYL010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.435"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            80..253
                     /locus_tag="OO572_004289"
     CDS             80..253
                     /locus_tag="OO572_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663040.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(325..>385)
                     /gene="hokB"
                     /locus_tag="OO572_004290"
     CDS             complement(325..>385)
                     /gene="hokB"
                     /locus_tag="OO572_004290"
                     /inference="COORDINATES: protein motif:HMM:NF013968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6663041.1"
                     /translation="LRFRDGDKEVAALMACTSR"
BASE COUNT          110 a           88 c           93 g           94 t
ORIGIN      
        1 gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca cataagattc
       61 gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg gaacgcttgt
      121 ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca
      181 ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac
      241 atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta gtcagatgcg
      301 cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg cagcaacctc
      361 cttatcaccg tcccggaacc gcagt
//
LOCUS       DAJTYL010000427          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTYL010000427 DAJTYL010000000
VERSION     DAJTYL010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.436"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            55..>384
                     /gene="ynaI"
                     /locus_tag="OO572_004291"
     CDS             55..>384
                     /gene="ynaI"
                     /locus_tag="OO572_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6663042.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGR"
BASE COUNT          111 a           71 c           78 g          124 t
ORIGIN      
        1 cccgccaggg gtttccggca tgacgacaat tatctggagg agcctgataa aacaatgatc
       61 gctgaactgt ttacaaataa tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca
      121 ttgattctga tgagcttttg gtttcgccgt ggaaatcgta aaagaaaagg atttttattc
      181 catgcggtgc aatttttaat ctacaccata attatcagtg ctgttggtag catcagtaat
      241 tatgttatag aaaactataa actcaaattt atcaccccag gcgttatcga ttttatctgt
      301 acgtccctga ttgcggttat tctgacgatt aagctatttc tgctgattaa tcagtttgaa
      361 aaacagcaga ttaaaaaagg tcgc
//
LOCUS       DAJTYL010000428          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTYL010000428 DAJTYL010000000
VERSION     DAJTYL010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.437"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            139..>381
                     /locus_tag="OO572_004292"
     CDS             139..>381
                     /locus_tag="OO572_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HCR6663043.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGEL"
BASE COUNT          113 a           84 c           90 g           94 t
ORIGIN      
        1 tgaaacctgc ccgaacgaac tggtttaatc ttgcagctcc cctgcaatcc aggggagctg
       61 attcagataa tccccaatga cctttcattc tctattctta aaatagtcct gagtcagaaa
      121 ctgtaattga gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg
      181 gcgggatgtg taagtaatga caaaattgct gttacgccag aacagctaca gcatcatcgc
      241 tttgtgctgg aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc
      301 agctttggtg aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc
      361 aaactgtcta atggtgaact g
//
LOCUS       DAJTYL010000429          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTYL010000429 DAJTYL010000000
VERSION     DAJTYL010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.438"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(100..>380)
                     /locus_tag="OO572_004293"
     CDS             complement(100..>380)
                     /locus_tag="OO572_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCR6663044.1"
                     /translation="AGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQHLIF
                     MIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT           95 a          110 c           85 g           90 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca
//
LOCUS       DAJTYL010000430          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTYL010000430 DAJTYL010000000
VERSION     DAJTYL010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.439"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..154)
                     /locus_tag="OO572_004294"
     CDS             complement(<1..154)
                     /locus_tag="OO572_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR6663045.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGL"
     gene            complement(193..>380)
                     /locus_tag="OO572_004295"
     CDS             complement(193..>380)
                     /locus_tag="OO572_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6663046.1"
                     /translation="GGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGV
                     NVFIQDVPGDQKEQIPD"
BASE COUNT          114 a           84 c           74 g          108 t
ORIGIN      
        1 ttaagccagt ctgaatatca ccgagaatag cgccagttag ggtacaaacg attatcgggc
       61 ggaataaaaa taaggcttcc agccaaaaat caatccccag aacgaaaact aacgccgcta
      121 gggataatcc ctgaagtagg gttatttcat gcatttttat tacctcaaac caatattaag
      181 gcgattttag atttagtcag ggatttgttc tttttgatcg ccagggacgt cctgaatgaa
      241 aacattcacg ccacgttgtt taataaaacg taagtccgcg agatcctggt catcgacata
      301 aactttactg ctgatttgct ttttcccttc cgagaaatgc atattgccga cgttgacatc
      361 tttcaggtca atgccacctt
//
LOCUS       DAJTYL010000431          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTYL010000431 DAJTYL010000000
VERSION     DAJTYL010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.440"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>377)
                     /locus_tag="OO572_004296"
     CDS             complement(<1..>377)
                     /locus_tag="OO572_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663047.1"
                     /translation="DDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIW
                     QYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVN
                     GVHGHGKACPLNEPILLADGTWT"
BASE COUNT           92 a          100 c           88 g           97 t
ORIGIN      
        1 tagtccatgt cccatcagct aaaagaatag gctcattcag tgggcatgcc tttccgtgac
       61 cgtgcacacc attgaccagc gtcaactctg cctcacggaa ctggaattta tctgccagag
      121 attcccacgg tggattaaac agatactgct gcttgccgta gaaagcgttg atagtgtcct
      181 ggtaaaactc tcgcgcactg tagagttctt caggatcgaa gtaggatgcc gtgccgatgt
      241 actgccagat ttcatcctcg gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg
      301 gcagagtaac aagacggcaa cgatgttcac cgagtcggct tacgatttcc cttgcggctt
      361 cacgaccaac atcatca
//
LOCUS       DAJTYL010000432          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTYL010000432 DAJTYL010000000
VERSION     DAJTYL010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.441"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            260..318
                     /locus_tag="OO572_004297"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO572_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTYL010000433          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTYL010000433 DAJTYL010000000
VERSION     DAJTYL010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.442"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>375
                     /locus_tag="OO572_004298"
     CDS             <1..>375
                     /locus_tag="OO572_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6663048.1"
                     /translation="VMAGNDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPG
                     TLNDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAE
                     QAKNDIDAALTGTLKTANHLSE"
BASE COUNT           98 a           90 c          119 g           68 t
ORIGIN      
        1 atgtgatggc aggaaacgat ggtgaatact gtttccatgc gcggactgga aaatatggtg
       61 tgtatctgaa acaggactgg cgcaacgagt acaacgttgg cgacattgct gtatatgagg
      121 actcaaagcc cggcacgctg aatgactttc tgattgctcc tgatgagggc gacctgaaac
      181 cggatgtcgt caaacgcttt gaggaaatgg tggcgcaggc gcagcagagc gccggggccg
      241 cagccggaaa cgcacagcag acggcgcagg atgtggcggc agccgcaggt tatgcccgcg
      301 cagcagagca ggccaaaaat gacattgatg ctgcgctgac cggcactctg aaaacggcta
      361 accatctgtc tgaaa
//
LOCUS       DAJTYL010000434          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTYL010000434 DAJTYL010000000
VERSION     DAJTYL010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.443"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(266..>374)
                     /locus_tag="OO572_004299"
     CDS             complement(266..>374)
                     /locus_tag="OO572_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6663049.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          105 a           84 c           76 g          109 t
ORIGIN      
        1 aagacaggag tcaagttccc gctccgacat aactgcgtgc aatggatttt gaaggaaccg
       61 tcagcccatt ccccggaggc aaacatgggc tggcaaaagt gtagcggtat taggcacagc
      121 tatttaggaa agagggacca tctccatctc attaaatgga ccacgccaga caattcactg
      181 tcatgctgat gctgcactgt ggggacagaa tcactcatga tgacttcgat aatcgacgac
      241 attattattt ggccgagctt ttaggtcata tctcaaatcg tttgcctaat attgctccat
      301 tttttaataa aaaatcaatc atttttgaat ctttgctttt cattgcattg tcaagcatag
      361 tgtccccact attt
//
LOCUS       DAJTYL010000435          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTYL010000435 DAJTYL010000000
VERSION     DAJTYL010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.444"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            33..>373
                     /gene="mug"
                     /locus_tag="OO572_004300"
     CDS             33..>373
                     /gene="mug"
                     /locus_tag="OO572_004300"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6663050.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGITKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYE"
BASE COUNT           91 a           80 c          106 g           96 t
ORIGIN      
        1 acttttcctg gtgatgttag aaaggagtaa atatggttga ggatattttg gctccagggt
       61 tacgggtcgt gttttgcggt atcaaccctg ggctttcatc cgccgggact ggttttccct
      121 ttgctcatcc ggcaaatcgc ttctggaagg tgatatatca ggccgggttt accgaccgtc
      181 agttgaagcc gcaggaggca cagcatctgc tggattatcg ttgtggcatc accaaactgg
      241 tagaccgtcc aacggtgcaa gccaatgaag tttcaaagca ggagctgcac gcaggtgggc
      301 gtaagctgat tgaaaaaatt gaagattatc agccgcaggc gttggctatt ctgggcaaac
      361 aagcatatga aca
//
LOCUS       DAJTYL010000436          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTYL010000436 DAJTYL010000000
VERSION     DAJTYL010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.445"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>373)
                     /locus_tag="OO572_004301"
     CDS             complement(<1..>373)
                     /locus_tag="OO572_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6663051.1"
                     /translation="ITGVAILNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTA
                     VNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLPGNRVLLSRIPVVPDS
                     NLLLAPAIDTVNRLPILLYQS"
BASE COUNT           91 a           85 c           98 g           99 t
ORIGIN      
        1 tggctctgat agagcaggat aggtaatcta ttaacggtgt cgattgccgg agccaacagt
       61 aaattactgt ccgggacaac aggaatacga gacagcagga cccgattccc aggcagggat
      121 gtacaccaga ctattccctg tttgagaatg atgattgtgc gcaggtgagg tttcagagct
      181 gcttcagtgc caagctgata ttgctcctcg aggtcgcatt ccttcccggc aatgttaacc
      241 gctgtttgag tcgcctgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat
      301 cttgctcccg ccaggtactc tgcttttgcg gaataccaga gctggatgtt caaaatagcg
      361 acaccggtaa taa
//
LOCUS       DAJTYL010000437          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTYL010000437 DAJTYL010000000
VERSION     DAJTYL010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.446"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..311)
                     /locus_tag="OO572_004302"
     CDS             complement(<1..311)
                     /locus_tag="OO572_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6663052.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTYL010000438          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTYL010000438 DAJTYL010000000
VERSION     DAJTYL010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.447"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(15..179)
                     /locus_tag="OO572_004303"
                     /pseudo
     CDS             complement(15..179)
                     /locus_tag="OO572_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT          106 a           92 c           63 g          107 t
ORIGIN      
        1 atcatcgatg actaaatcac atacattgat gtcgtccact cagacagcgg aatattgtac
       61 tgttctataa cgccgccgat ggtcgcgcga gtcacaaccg acgccgcaat aatgaaaacc
      121 gccaacaaac caaagagaaa tttcttccct ttcgctgact tcagtttcgc cataatcatg
      181 atgtaatcct tgtgcagaca aatttcttgt tacctaaacg taacaatacc atgacgtttc
      241 accgttgtct gctctgaaaa cctcacctct tagctggatt tacaatcaac gcattgatta
      301 gttagctaat gttgctactt ccttccctga ctattgatgt tctgaatgaa ttgtcgcggt
      361 aaaatagc
//
LOCUS       DAJTYL010000439          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTYL010000439 DAJTYL010000000
VERSION     DAJTYL010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.448"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..343
                     /locus_tag="OO572_004304"
     CDS             <1..343
                     /locus_tag="OO572_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6663053.1"
                     /translation="RARSDAFYDASNSSPLDSLNPRKIFISASGVHNHFGVSWFNPED
                     LATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRPLPADYVTHCQNGS
                     VKIITPDSEDE"
BASE COUNT           94 a           93 c           78 g          100 t
ORIGIN      
        1 tcgtgccaga agtgatgctt tttacgatgc cagtaactct tcgccattag actctctcaa
       61 tccgcgaaaa atatttattt ccgccagcgg tgtgcataat cactttggcg tcagctggtt
      121 taaccctgaa gatcttgcca ctaagcgtaa agtgatgaac cgtggactac ggaaaatttt
      181 gctcgcccgc cacgcgttgt tcgatgaagt ggcctctgcc agcctcgcac cgatctctgc
      241 atttgacgtt ctgattagcg atcgtccgtt accggcagat tatgttacgc actgccagaa
      301 tggttctgta aagatcatta cacctgattc agaagacgaa tgacttactg aaaaaacacc
      361 acaat
//
LOCUS       DAJTYL010000440          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTYL010000440 DAJTYL010000000
VERSION     DAJTYL010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.449"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>360
                     /locus_tag="OO572_004305"
     CDS             <1..>360
                     /locus_tag="OO572_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6663054.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDII"
BASE COUNT          112 a           82 c           75 g           91 t
ORIGIN      
        1 aattccatct gattactggc aatattgcgc aatataaaga tcggctgtat gtaacaaaat
       61 tatcgacatt tggtgaagac cagcttgaga taatcccgct ggatacgcca gacctggtca
      121 ttgatcgctc atttaatagc ggcaaacagc atgcttactt tatccgccaa ttacggtcaa
      181 agagcttgca aattattcca gttaacggtt cgctaactaa aaacgatcgc ttcgcttatg
      241 acgatcgcaa tgtttataca tggaccgata cagaggtaag gattacgccc tccccctgcc
      301 cggcgaaaac tcgtgtcaga gaggagaacg tacgtgaagt tcaaaacaga gacatcatta
//
LOCUS       DAJTYL010000441          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTYL010000441 DAJTYL010000000
VERSION     DAJTYL010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.450"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          123 a           82 c           56 g           97 t
ORIGIN      
        1 ccccatgaaa acgcatctta atgagcaaaa ttcttcgaaa tggcttatcc acaagtaact
       61 ctttttgttg agattatccg attaaggtga agcagaaaaa gcacgctacc gccccaggct
      121 caacagctac ctgaatactg ataccgcact gatattgacg cgctagtgta tcacttcttt
      181 cgcggcaatc cgacccgaat gatttttaag cacattgttg attttcaata aagaaatcaa
      241 tcaaataaaa tcattagcaa ccatattctt tcactgttgc tctgcgattc cttcctgcac
      301 gtacagcaga aatcaatgaa tcgcataaaa atacaaaaaa agtatagatg ttcaacaa
//
LOCUS       DAJTYL010000442          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTYL010000442 DAJTYL010000000
VERSION     DAJTYL010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.451"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            39..>357
                     /locus_tag="OO572_004306"
     CDS             39..>357
                     /locus_tag="OO572_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6663055.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAJTYL010000443          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTYL010000443 DAJTYL010000000
VERSION     DAJTYL010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.452"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>355
                     /locus_tag="OO572_004307"
     CDS             <1..>355
                     /locus_tag="OO572_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6663056.1"
                     /translation="KIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDS
                     IPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAG
                     NPLSGHTMRTLQQITT"
BASE COUNT          114 a           79 c           62 g          100 t
ORIGIN      
        1 aagataaaag aattaccatt tcttcctgaa aatctaactc atttacgggt tcataataac
       61 cgattgcata ttctgccact attgccaccg gaactaaaat tactggtagt ttctggaaac
      121 agattagaca gcattccccc ctttccagat aagcttgaag ggctggctct ggctaataat
      181 tttatagaac aactaccgga attacctttt agtatgaaca gggctgtgct aatgaataat
      241 aatctgacaa cacttccgga aagtgtcctg agattagctc agaatgcctt cgtaaatgtt
      301 gcaggtaatc cattgtctgg ccataccatg cgtacactac aacaaataac caccg
//
LOCUS       DAJTYL010000444          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTYL010000444 DAJTYL010000000
VERSION     DAJTYL010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.453"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>351
                     /locus_tag="OO572_004308"
     CDS             <1..>351
                     /locus_tag="OO572_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663057.1"
                     /translation="GKESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVI
                     LNEEGTFNNIYLASGNGKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAA
                     TDDGTTITNSDTKK"
BASE COUNT          138 a           53 c           72 g           88 t
ORIGIN      
        1 cggaaaagaa agcattgtaa actggaatgc attgtattcc agtgatgatg ttttacacaa
       61 aataggcccc ggtactctga atgttcaaaa aaaacagggg gcaaatataa agataggtga
      121 aggaaatgtt attcttaatg aagaaggaac atttaacaat atataccttg caagcggaaa
      181 tggtaaggta atactaaata aagataattc ccttggcaat gatcaatatg cggggatatt
      241 ttttactaaa cgtggtggca cgctagattt aaatggacac aatcagactt ttactagaat
      301 tgccgccact gacgatggaa caacaataac taactcagat acaaagaaag a
//
LOCUS       DAJTYL010000445          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTYL010000445 DAJTYL010000000
VERSION     DAJTYL010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.454"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>348)
                     /locus_tag="OO572_004309"
     CDS             complement(<1..>348)
                     /locus_tag="OO572_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCR6663058.1"
                     /translation="IVAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEG
                     NLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQ
                     IFIAIEVDKLIDGP"
BASE COUNT           91 a           91 c           89 g           77 t
ORIGIN      
        1 gggaccgtcg ataagcttgt ccacttcaat ggcaataaaa atttgtgaaa tgccgtattc
       61 gtcgctgttg tcctgggtga cttcggcaac cgatgcgccg tcggaaagga gagtagcgat
      121 catatccagc acaatcgaca tgccagaacc tttccagtag cccatcggca aaatgcggcg
      181 attcttctcg ataacgccag gttctttggt caaattgccc tcatcatcaa agccaccatc
      241 gaccgggagc tgacggcctg ccaggcggtt aacttctaac atgccgtaag agaacatcga
      301 catcgacata tcgaccatgg tgatcggcgt ggaaggaatg gcgacgat
//
LOCUS       DAJTYL010000446          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTYL010000446 DAJTYL010000000
VERSION     DAJTYL010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.455"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT          102 a           96 c           65 g           84 t
ORIGIN      
        1 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
       61 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
      121 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
      181 catttaaatt tcacagacaa gaccgactct ttatggatac cggaaatgcg ccttcgtgtt
      241 gtgcccggtt ttatttcacc acttccgggc tttggtggta tctttactga agttctcaca
      301 caaccagtaa ggaaatgaat atgccaacgt atctcgccag agtagaa
//
LOCUS       DAJTYL010000447          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTYL010000447 DAJTYL010000000
VERSION     DAJTYL010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.456"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT           97 a           68 c           67 g          114 t
ORIGIN      
        1 catttcgact ataactgaca ggtcatcacg acgtatgact gcgtatttgc tactaaatag
       61 tttctttatt ttttccacga tgcccccagg tttataagta caaacggtta tatccacata
      121 gagacaaaaa tattaatctg ataaatattt atttcacgcc gtatatttga ttgtttaatg
      181 tgcaagtaca atgactttta ttttttgttg tgtatataat caaatatatg gttatttttc
      241 accctgcgta ttcagcacgc aacaaaaaac ccgccgaagc gggtttagtg cgggtgcgtt
      301 gaggatgcct gacacatgag aggtggcgag ggatttctcc cccgcc
//
LOCUS       DAJTYL010000448          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTYL010000448 DAJTYL010000000
VERSION     DAJTYL010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.457"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..214
                     /locus_tag="OO572_004310"
     CDS             <1..214
                     /locus_tag="OO572_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6663059.1"
                     /translation="LRERIQQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALH
                     AADIAMYQEKKHKQKTPFVAHPALHS"
     gene            247..345
                     /locus_tag="OO572_004311"
     CDS             247..345
                     /locus_tag="OO572_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6663060.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
BASE COUNT           91 a           73 c           79 g          102 t
ORIGIN      
        1 gctgcgagaa cgtattcaac agcaaatacg tggagaatat cacttaggtg atgttgattt
       61 gtattatccc ggtgccagtc ttggcatagt agaagtcgat cctgaaacaa ccgatgcaga
      121 cagtgccctg catgctgccg atattgcgat gtatcaggag aaaaaacaca aacagaaaac
      181 accttttgtc gcgcatccag cgctacattc ctgaggcgta ttcacatcct tttgattggt
      241 gataacatgc gaatcggtat tatttttccg gttgtaatct tcattacagc ggtcgtattt
      301 ttagcatggt tttttattgg ctgctatgct gccccgggag cataa
//
LOCUS       DAJTYL010000449          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTYL010000449 DAJTYL010000000
VERSION     DAJTYL010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.458"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            151..306
                     /locus_tag="OO572_004312"
     CDS             151..306
                     /locus_tag="OO572_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6663061.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          107 a           82 c           69 g           87 t
ORIGIN      
        1 gtgtttttta catcaaaaaa cttaggtttt tctacacaaa aatcttgatc accctcctta
       61 ggttattcta aattttattc atcaagacac cgcacggtgt tctcagcaaa cagttccgct
      121 accccggcgt taaggggaaa tggggtcaac atgaatacta tcgatcttgg caacaacgaa
      181 tctccggtgt acggcgtgtt ccccaaccag gacggcacat tcaccgccat aacgtatacc
      241 aaaagcaaaa cgtttaaaac cgaagctggc gcacgtcgtt ggttgggaag acattcaggt
      301 gagtaaaatg aacgagacag aattaaaaca cgttatcgct ctact
//
LOCUS       DAJTYL010000450          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTYL010000450 DAJTYL010000000
VERSION     DAJTYL010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.459"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(148..>343)
                     /locus_tag="OO572_004313"
     CDS             complement(148..>343)
                     /locus_tag="OO572_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6663062.1"
                     /translation="QLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLIS
                     AASWSALYVGSFSFVLPFFS"
BASE COUNT          113 a           69 c           81 g           80 t
ORIGIN      
        1 accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt ggggggtagc
       61 ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg ggggaagctg
      121 gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga agctaaaact
      181 accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat acactttcac
      241 tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca cggcgataaa
      301 gcgcgaagtg aaaagtattg acacagagtt attttgcaac tga
//
LOCUS       DAJTYL010000451          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTYL010000451 DAJTYL010000000
VERSION     DAJTYL010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.460"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>342
                     /locus_tag="OO572_004314"
     CDS             <1..>342
                     /locus_tag="OO572_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR6663063.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLR"
BASE COUNT          104 a           72 c           78 g           88 t
ORIGIN      
        1 tagtaaactg gaaaaataat cccgacggca cgcgcagtcc ctatgagatg aatgtcacct
       61 atatgaatgc gttaagccgc cgcgagagta gcgatgaaga acgttgcacc aggtttatcc
      121 ttgcccatgc gattttgtta agtttccccg gtgtacccgc gatatatatt caaagtattc
      181 ttggctcgcg taatgattac gcaggtgtcg aaaaactcgg atataaccgt gcgattaacc
      241 gtaaaaaata tcacagtgaa gagataacac gagaactgaa cgatgaagct acattaaggc
      301 atgcggtata tcatgagttg tcgcgtttaa ttacacttcg tc
//
LOCUS       DAJTYL010000452          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTYL010000452 DAJTYL010000000
VERSION     DAJTYL010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.461"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>340)
                     /locus_tag="OO572_004315"
     CDS             complement(<1..>340)
                     /locus_tag="OO572_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6663064.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTYL010000453          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJTYL010000453 DAJTYL010000000
VERSION     DAJTYL010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.462"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(155..>334)
                     /locus_tag="OO572_004316"
     CDS             complement(155..>334)
                     /locus_tag="OO572_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663065.1"
                     /translation="NVSLERNQLLSLASSPATRSELKVTFDNAPPGMRVIDLPKTGDP
                     FMKITHDVGYSPFKR"
BASE COUNT           98 a           76 c           64 g           96 t
ORIGIN      
        1 tgaaacaatc tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc
       61 aaccgtcatt aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat
      121 aattttgcct catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa
      181 catcatgggt gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc
      241 caggcggcgc gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa
      301 ggcttaacag ctgattcctt tccagtgata catt
//
LOCUS       DAJTYL010000454          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJTYL010000454 DAJTYL010000000
VERSION     DAJTYL010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.463"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            51..>332
                     /locus_tag="OO572_004317"
     CDS             51..>332
                     /locus_tag="OO572_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6663066.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTYL010000455          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTYL010000455 DAJTYL010000000
VERSION     DAJTYL010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.464"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>330
                     /locus_tag="OO572_004318"
     CDS             <1..>330
                     /locus_tag="OO572_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HCR6663067.1"
                     /translation="DNKRSKQADVQASAVSENNKVLGFLREKGCDYCHTPSAELPAYY
                     YIPGAKQLMDYDIKLGYKSFNLEAVRAALLADKPVSQSDLNKIEWVMQYETMPPTRYT
                     ALHWAGKV"
BASE COUNT           93 a           73 c           81 g           83 t
ORIGIN      
        1 cgataataaa cgcagtaaac aggccgatgt tcaggcatct gctgtcagtg aaaataataa
       61 ggttttaggc tttctccgcg aaaaaggatg cgactattgc cacacgcctt cggcagaatt
      121 acccgcctat tattatattc ctggcgcgaa acagttgatg gattacgaca ttaagcttgg
      181 atataaatct tttaacctcg aggccgtgcg tgcggcactg ctggctgata aacccgtttc
      241 gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat gaaactatgc caccaacgcg
      301 ttataccgcg ctacactggg cgggtaaggt
//
LOCUS       DAJTYL010000456          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTYL010000456 DAJTYL010000000
VERSION     DAJTYL010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.465"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..283)
                     /locus_tag="OO572_004319"
     CDS             complement(<1..283)
                     /locus_tag="OO572_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR6663068.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNT"
BASE COUNT          100 a           84 c           72 g           67 t
ORIGIN      
        1 acgtattacg cccggcaagc gacatcaacg tcaggatccc cccctcaccg gcgttatctg
       61 cccgcatcac gaaggtgaga tatttaatgg aaaccacaaa gattagcagc cagaagatca
      121 gcgataaaaa gccaaacacg gcatcgcgtt caacgccaaa accaaactgg ccggacaaac
      181 attcacgaag tgtatataac gggctggtac caatatcgcc gtagacaact ccaatcgccg
      241 cgagggtaat cgcgggcaat gattgcttat tatcagtgct catagactag tctttcgttg
      301 aaatatgaaa tgtgtgctta gtc
//
LOCUS       DAJTYL010000457          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJTYL010000457 DAJTYL010000000
VERSION     DAJTYL010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.466"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..62
                     /locus_tag="OO572_004320"
     CDS             <1..62
                     /locus_tag="OO572_004320"
                     /inference="COORDINATES: protein motif:HMM:NF027269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6663069.1"
                     /translation="NTLLACCCLSRPDEVNSEY"
     gene            52..225
                     /locus_tag="OO572_004321"
     CDS             52..225
                     /locus_tag="OO572_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6663070.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
BASE COUNT          105 a           65 c           73 g           79 t
ORIGIN      
        1 ccaacacctt actggcgtgt tgctgtcttt caagaccaga cgaggttaac agtgaatatt
       61 gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc
      121 gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcatcgc acgagaaaaa
      181 atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac
      241 cccgtgcgag gtgatgttcc gcttgttgct aatttagtga ccaatcattg gcgcttgtgg
      301 aattaagcgt cggtacaatt cc
//
LOCUS       DAJTYL010000458          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJTYL010000458 DAJTYL010000000
VERSION     DAJTYL010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.467"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>322)
                     /locus_tag="OO572_004322"
     CDS             complement(<1..>322)
                     /locus_tag="OO572_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6663071.1"
                     /translation="EKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVA
                     ESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQ
                     RLGSV"
BASE COUNT           74 a           87 c           63 g           98 t
ORIGIN      
        1 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgttcgct
       61 agaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcactgcg
      121 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
      181 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
      241 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
      301 atgaaccagc acctgttttt cg
//
LOCUS       DAJTYL010000459          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJTYL010000459 DAJTYL010000000
VERSION     DAJTYL010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.468"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>317
                     /locus_tag="OO572_004323"
     CDS             <1..>317
                     /locus_tag="OO572_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6663072.1"
                     /translation="RERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDT
                     ALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVL
                     WNW"
BASE COUNT           82 a           72 c           85 g           78 t
ORIGIN      
        1 ctcgcgaacg aaaactggta tcaggcttcg ttgattacct gtgcgaatca gtaggtcggg
       61 aacacactcc agccctgagt gatgccgcag agcaatacct taatacagtt gcgacatgca
      121 gaacccggga tacggcattg ctaaagtcgt ttgacgcttt ccgcgagtgg gtaaccattc
      181 aggccggatt ttacaccgag catatttatc ctgatggtag tcgtgggcgc agggcaaaat
      241 ctatcgcatt tgcgaacatg gacgaaaccg agtttcagca ggtttataaa tctgtactga
      301 atgtgctgtg gaactgg
//
LOCUS       DAJTYL010000460          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJTYL010000460 DAJTYL010000000
VERSION     DAJTYL010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.469"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
BASE COUNT           93 a           62 c           66 g           95 t
ORIGIN      
        1 cttgaaacgc attatttccg ggggcataaa aatgcgctct gcatttttgc tttctttttc
       61 acctgtcatg cccaatctct ctgttatata aagaatttta cagaattctt tgatttgcac
      121 tggttaaaat aaaaatgcgc atagcttttg aattgagcat ttttctgcgc tatcgttttt
      181 cccgatagcg gtagccggta tcagttaccg aaaacgttaa gagagtaagg atcgcagaat
      241 gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc gtatgaaggg
      301 gaagacacta tctcaa
//
LOCUS       DAJTYL010000461          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJTYL010000461 DAJTYL010000000
VERSION     DAJTYL010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.470"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>312
                     /locus_tag="OO572_004324"
     CDS             <1..>312
                     /locus_tag="OO572_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6663073.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     F"
BASE COUNT           74 a           81 c           77 g           80 t
ORIGIN      
        1 gcttgagcta ctctcacttc aacaacgacc tgtctgcaac catgagcaac ggtacttacg
       61 ttgacggtag caccaactcc gacgcttggg gcttcggttt gaaaaccggt tacgacttca
      121 aactgggtga tgctggttac gtgactcctt acggcagcat ttctggtctg ttccagtctg
      181 gtgatgacta ccagctgagc aacgacatga aagttgacgg tcagccttac gacagcatgc
      241 gttatgaact gggtgtagat gcaggttata ccttcaccta cagcgaagat caggctctga
      301 ctccgtactt ca
//
LOCUS       DAJTYL010000462          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJTYL010000462 DAJTYL010000000
VERSION     DAJTYL010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.471"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(96..275)
                     /locus_tag="OO572_004325"
     CDS             complement(96..275)
                     /locus_tag="OO572_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663074.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
BASE COUNT           65 a           67 c           71 g          107 t
ORIGIN      
        1 gcctgtaaca gttgctccag cctttttatt ttgttctcat tcatccggtt ttcctcgtat
       61 gctgcatcac accactgtgc taatgaaaaa tgtaatcagt gtggtggacg tgttttatcc
      121 gcattggaat tgtcgtcaac taaaggagat gaggcccgtt tgtatcgcaa tgggtctatg
      181 gcgctcatgc ccgtttgctg ttcgataatg gcttctctga gcatacggtg ttgcagttct
      241 cgtaaaaact ttgcgtcttc tggtgataaa tccattgtta ttactcgtaa agcaatgcgc
      301 tgtagtgcct
//
LOCUS       DAJTYL010000463          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJTYL010000463 DAJTYL010000000
VERSION     DAJTYL010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.472"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..93
                     /gene="yoeB"
                     /locus_tag="OO572_004326"
     CDS             <1..93
                     /gene="yoeB"
                     /locus_tag="OO572_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6663075.1"
                     /translation="FWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            176..>309
                     /locus_tag="OO572_004327"
     CDS             176..>309
                     /locus_tag="OO572_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6663076.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     "
BASE COUNT           80 a           61 c           87 g           81 t
ORIGIN      
        1 ttctggtccc gacgcattac agaggagcac cgtctggtat acgcggttac tgatgattca
       61 ctgctcattg cagcatgtcg ttatcattat tgaacagatt cattcataga atgaattgtc
      121 agttttgata cgctagcgta gccttataga tctaaggtgt atcaggagat aacggatgaa
      181 aaaggtcgca attgtcgggt tagggtggtt aggtatgccg ctggcgatgt cactttcagc
      241 gcgaggctgg caagtcaccg ggagtaaaac cacacaagat ggtgtcgaag cggcccgaat
      301 gagtggcat
//
LOCUS       DAJTYL010000464          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJTYL010000464 DAJTYL010000000
VERSION     DAJTYL010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.473"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>307
                     /locus_tag="OO572_004328"
     CDS             <1..>307
                     /locus_tag="OO572_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6663077.1"
                     /translation="PNRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVV
                     LGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS
                     "
BASE COUNT           71 a           66 c           90 g           80 t
ORIGIN      
        1 agcctaaccg taaccgtctg ctgtggagca gcaatctgaa tgttgatggc atgaagacag
       61 gaaccacggc aggtgcggga tataatctgg ttgcttcggc tacccagggt gatatgcgtt
      121 taatctccgt agtgctgggg gcgaaaactg accgtatccg ttttaatgag tctgagaaat
      181 tattgacctg gggtttccgc ttctttgaaa ctgtgacgcc aattaaacct gatgccacct
      241 ttgtgactca gcgcgtctgg tttggtgata agatcgaagt gaatctcggg gcaggcgaag
      301 cgggctc
//
LOCUS       DAJTYL010000465          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJTYL010000465 DAJTYL010000000
VERSION     DAJTYL010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.474"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>303)
                     /locus_tag="OO572_004329"
     CDS             complement(<1..>303)
                     /locus_tag="OO572_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6663078.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLG"
BASE COUNT           76 a           98 c           65 g           64 t
ORIGIN      
        1 cacccagcgg cggccagatc ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg
       61 actccagccc ttcccttgca acggcaaaaa agaccatcat caccagcgcc cagccatgat
      121 gatttccacg ctgcaatgcg ctatcgactg cctgttccag ttgcactttg acgttgcgcg
      181 acactttgcg catccagaaa accatccagg taaggatcac cacggcgatc accgccacga
      241 taccttcaaa cagttcctgc tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga
      301 tcc
//
LOCUS       DAJTYL010000466          299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJTYL010000466 DAJTYL010000000
VERSION     DAJTYL010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.475"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            100..>299
                     /locus_tag="OO572_004330"
     CDS             100..>299
                     /locus_tag="OO572_004330"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6663079.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISES"
BASE COUNT          100 a           60 c           56 g           83 t
ORIGIN      
        1 cctttctccc aagtgaaaat aaaaggttat cagtttgcaa cattgaacaa cattcgttgc
       61 aaatcgataa caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc
      121 acccgcgtaa aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa
      181 aatgatttga gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag
      241 cttatcgcgt gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatc
//
LOCUS       DAJTYL010000467          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJTYL010000467 DAJTYL010000000
VERSION     DAJTYL010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.476"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>297
                     /locus_tag="OO572_004331"
     CDS             <1..>297
                     /locus_tag="OO572_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR6663080.1"
                     /translation="LMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLT
                     ITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDA"
BASE COUNT           60 a           75 c           74 g           88 t
ORIGIN      
        1 ctgatgatgt ggggagcaat gcgctatggt tataagctga tttcgctgct ctgggcggtc
       61 gtgttgatga tcagcatcca cagctatcaa aattacattc ccatttatcc tggctatacc
      121 acgcagctga ccataacctc ctccagttat ctggtattct cttttattgt caattatatg
      181 gctgtactgg caactcgtca gcgagcggta gtcagacgca ttcagcggct tgcgtatgtg
      241 gacccggtgg ttcatctgcc aaatgttcgc gccctgaatc gcgcgttgcg tgatgcc
//
LOCUS       DAJTYL010000468          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJTYL010000468 DAJTYL010000000
VERSION     DAJTYL010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.477"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..>297)
                     /locus_tag="OO572_004332"
     CDS             complement(<1..>297)
                     /locus_tag="OO572_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6663081.1"
                     /translation="DFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNM
                     IGYSRLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATG"
BASE COUNT           81 a           80 c           52 g           84 t
ORIGIN      
        1 tccggttgct ttagcgaata cctgaaccat accaggaaca gcttcagcta gttgcccctt
       61 aagctcttca gccataatct gacccttacc agccatctgt gataatgctg tcagtgctcg
      121 cttaatctct tcttcacttc gccccatcag acgagaatag ccaatcatat ttctaaaaag
      181 ttcttcagaa ccttgaatgc ctagagactc tctagctgaa gcaccgaaac cagcaaactg
      241 tgattgcagt tcagcgatat ctaaacccgt tgtttgagct aatgactttg tgaaatc
//
LOCUS       DAJTYL010000469          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJTYL010000469 DAJTYL010000000
VERSION     DAJTYL010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.478"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..213
                     /locus_tag="OO572_004333"
     CDS             <1..213
                     /locus_tag="OO572_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6663082.1"
                     /translation="KKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPT
                     KAKNYQRYNERIKGKRQEWGILCNND"
BASE COUNT           99 a           64 c           71 g           61 t
ORIGIN      
        1 aaaaaaggca tcgaggttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc
       61 tcctggcggc ggatatgcaa agttttactc aataacgatt actggtgtcg ggcattatca
      121 tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaaaacgt
      181 caggaatggg ggatactatg caacaacgat taacgcagga tttaacgcaa tttctcgcca
      241 gcttgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg atcca
//
LOCUS       DAJTYL010000470          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJTYL010000470 DAJTYL010000000
VERSION     DAJTYL010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.479"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(55..243)
                     /locus_tag="OO572_004334"
     CDS             complement(55..243)
                     /locus_tag="OO572_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6663083.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDIGG"
BASE COUNT           74 a           72 c           87 g           62 t
ORIGIN      
        1 cgtgaacaga cgtttcagcg ggcggggagt aaaagcggac aacatgagga cggattaacc
       61 cccgatatcc tgtagccggg taagggctgc tgtgtcgcca cggcgctcca ggatggaggc
      121 cgcgaccatc gcaggtggtg ggtccatggg tttgagcatc ttcatccagt cccattcacg
      181 ttgtgagagc ttctcagcac caacagagag cagaacggta tctatacttt tgcgggtcaa
      241 cataagagga tgagtcctta ataaaacagg tcctcagagc atacatattc tgcgg
//
LOCUS       DAJTYL010000471          293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJTYL010000471 DAJTYL010000000
VERSION     DAJTYL010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.480"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..148
                     /locus_tag="OO572_004335"
     CDS             <1..148
                     /locus_tag="OO572_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic sulfatase maturase AslB"
                     /protein_id="HCR6663084.1"
                     /translation="KPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIMQAHLM
                     VVNK"
BASE COUNT           67 a           68 c           80 g           78 t
ORIGIN      
        1 caaaccgggg ctgaattatt tgtgtgccgg gtatcagcgt tatttccgcc atctaccgcc
       61 atatcttaaa gcaatggctg atttgctggc gcacggtcgc ccggccagtg acattatgca
      121 ggcacatttg atggtggtga ataagtaaaa aagcggccat ttggaagccg cctgcggttg
      181 attgccggat gcggcgtaaa cgccttatcc ggcctacatg atcgtgcaaa ttcaataaat
      241 tgcagcgttc tgtggatact ggtttgggtg atcgtatccg gcaaagcctt tac
//
LOCUS       DAJTYL010000472          291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJTYL010000472 DAJTYL010000000
VERSION     DAJTYL010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.481"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            66..>291
                     /gene="bolA"
                     /locus_tag="OO572_004336"
     CDS             66..>291
                     /gene="bolA"
                     /locus_tag="OO572_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6663085.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHAL"
BASE COUNT           78 a           56 c           78 g           79 t
ORIGIN      
        1 acggtaaaag cggctagtat ttaaagggat ggatgacatc tcagcgttgt cggaggagat
       61 atttcatgat gatacgtgag cggatagaag aaaaattaag ggcggcgttc caacccgtat
      121 tcctcgaagt agtggatgaa agctatcgtc acaatgtccc agccggctct gaaagccatt
      181 ttaaagttgt gctggtcagc gatcgtttta cgggtgaacg ttttctgaat cgtcatcgaa
      241 tgatttacag tactttagcg gaggaactct ctactaccgt tcatgcgctg g
//
LOCUS       DAJTYL010000473          291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJTYL010000473 DAJTYL010000000
VERSION     DAJTYL010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.denovo.482"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..>291
                     /locus_tag="OO572_004337"
     CDS             <1..>291
                     /locus_tag="OO572_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6663086.1"
                     /translation="DWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMATIPA
                     LSHPDVTIENGRAVTTSVAVAEFFHKRHDNVLRAIANTECSPEFNALNFEDVT"
BASE COUNT           67 a           85 c           62 g           77 t
ORIGIN      
        1 agactggccg ggttctcgag tgaccggtat ttccacccct gtacgtctca ccacccttat
       61 ggtcgtggaa aacctcggtg gtgagttaat taacttatca ctcgaggatg ccatcatggc
      121 tactatccct gccctttctc accctgacgt aaccatcgaa aacggtcgcg ctgtcactac
      181 gtctgttgcg gttgcagagt tcttccacaa acgacacgac aatgtgttac gtgccatcgc
      241 aaatactgaa tgctcacctg aatttaacgc cctcaatttt gaggacgtca c
//
LOCUS       DAJTYL010000474         5560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.179, whole genome shotgun sequence.
ACCESSION   DAJTYL010000474 DAJTYL010000000
VERSION     DAJTYL010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.179"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            <1..659
                     /gene="frdB"
                     /locus_tag="OO572_004338"
     CDS             <1..659
                     /gene="frdB"
                     /locus_tag="OO572_004338"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6663087.1"
                     /translation="YEVPYDATTSLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMV
                     NNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTA
                     DQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRD
                     HGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR
                     "
     gene            670..1065
                     /gene="frdC"
                     /locus_tag="OO572_004339"
     CDS             670..1065
                     /gene="frdC"
                     /locus_tag="OO572_004339"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6663088.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1076..1435
                     /gene="frdD"
                     /locus_tag="OO572_004340"
     CDS             1076..1435
                     /gene="frdD"
                     /locus_tag="OO572_004340"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6663089.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1498..2630
                     /locus_tag="OO572_004341"
                     /pseudo
     CDS             1498..2630
                     /locus_tag="OO572_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            2719..3252
                     /gene="blc"
                     /locus_tag="OO572_004342"
     CDS             2719..3252
                     /gene="blc"
                     /locus_tag="OO572_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6663090.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3249..3566)
                     /gene="sugE"
                     /locus_tag="OO572_004343"
     CDS             complement(3249..3566)
                     /gene="sugE"
                     /locus_tag="OO572_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6663091.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3742..3888)
                     /gene="ecnB"
                     /locus_tag="OO572_004344"
     CDS             complement(3742..3888)
                     /gene="ecnB"
                     /locus_tag="OO572_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6663092.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3999..4124)
                     /gene="ecnA"
                     /locus_tag="OO572_004345"
     CDS             complement(3999..4124)
                     /gene="ecnA"
                     /locus_tag="OO572_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6663093.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(4176..4742)
                     /gene="efp"
                     /locus_tag="OO572_004346"
     CDS             complement(4176..4742)
                     /gene="efp"
                     /locus_tag="OO572_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6663094.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4784..>5560
                     /gene="epmB"
                     /locus_tag="OO572_004347"
     CDS             4784..>5560
                     /gene="epmB"
                     /locus_tag="OO572_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6663095.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRG"
BASE COUNT         1371 a         1440 c         1414 g         1335 t
ORIGIN      
        1 tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag
       61 acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct
      121 gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca
      181 ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg
      241 atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc
      301 gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt
      361 tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc
      421 cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg
      481 accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggtgta tggagctgta
      541 ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc
      601 agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag
      661 gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg
      721 aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg
      781 tggttcagca ttgaactgat tttcgcgctg tttgccctga aaaatggccc ggaagcctgg
      841 gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg
      901 acggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc
      961 attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact
     1021 gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat
     1081 taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg
     1141 tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact
     1201 ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt
     1261 cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg
     1321 tatgcaccac gcgatgcacg atctgaaaat ccacgtgcct gcgggcaaat gggttttcta
     1381 cggtctggct gctatcctga cagttgtcac gctgattggt gtcgttacaa tctaacgcat
     1441 cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg
     1501 ttcaaaacga cgctctgcgc cttattaatt accgcctctt actccacatt tgctgcccct
     1561 caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc
     1621 cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat
     1681 gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc
     1741 agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta
     1801 agcgatcccg caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca
     1861 ctattacatc ttgcgaccta caccgctggc ggcctgccat tgcaggtgcc ggatgaagtg
     1921 aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg ggctccagga
     1981 acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg
     2041 tctggtttga gttttgagca ggcgatgcaa actcgtgtct tccagccact caaactcaac
     2101 catacgtgga ttaatgtacc tcccccagaa gaaaagaatt acgcctgggg atatcgcgaa
     2161 ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg aagcttatgg tgtgaagtcg
     2221 accattgaag atatggcccg ctgggtgcaa agcaatttac cccccttgat atcaatgaga
     2281 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
     2341 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     2401 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     2461 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     2521 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     2581 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     2641 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     2701 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     2761 ctggtcgttg cctgtagttt tcctacgccg ccgcgtggcg tgaccgtagt gaataatttc
     2821 gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     2881 cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc
     2941 attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac
     3001 tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     3061 ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac
     3121 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     3181 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     3241 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     3301 ctcgccagac gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg
     3361 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctaccggtaa cgatttcatc
     3421 gcccaggcaa gtagcgccat actgactatc atcgccgtca cggtaataac actcggcgtc
     3481 aaacggctaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     3541 gcaataacta agataatcca ggacatatca ggctccggaa atttggggcc gtccccggtg
     3601 aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     3661 ggcaggggaa tttcaactat tacctttatg acgatcgcag aaaaaacaaa cggcacgaca
     3721 ctgctgtcgt accgttattg cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt
     3781 accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt
     3841 tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt
     3901 ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa
     3961 tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg
     4021 gagttaccga gatgtttgat gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc
     4081 agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag
     4141 tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca
     4201 ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg aactttaacc
     4261 acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg
     4321 cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc
     4381 tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt agcattgtcg
     4441 ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat gaagtgccag
     4501 aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc gccttcagcg
     4561 gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg acgcagttta
     4621 acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc aaccgcgtaa
     4681 ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata gtacgttgcc
     4741 ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc atattgtaac
     4801 cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg tgaccgatcc
     4861 tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg ccggacgcag
     4921 cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg
     4981 caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc
     5041 gtccggattc tccaccgacc cactggaaga acagcacagc gtagtgcctg gtttgttgca
     5101 taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa attgccgcta
     5161 ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta actggcagac
     5221 tgcacttgag tatgttgctg cgcatccgga actggacgag atgattttct ccggcggcga
     5281 tccgctgatg gcgaaagagc acgagctgga ctggttgctc acacaactgg aagccatccc
     5341 gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg cacgtatcac
     5401 cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat
     5461 caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt
     5521 cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
//
LOCUS       DAJTYL010000475         5014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.187, whole genome shotgun sequence.
ACCESSION   DAJTYL010000475 DAJTYL010000000
VERSION     DAJTYL010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.187"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            48..707
                     /locus_tag="OO572_004348"
     CDS             48..707
                     /locus_tag="OO572_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6663096.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(908..1285)
                     /locus_tag="OO572_004349"
     CDS             complement(908..1285)
                     /locus_tag="OO572_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6663097.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1352..4318)
                     /locus_tag="OO572_004350"
     CDS             complement(1352..4318)
                     /locus_tag="OO572_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6663098.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4321..4881)
                     /locus_tag="OO572_004351"
     CDS             complement(4321..4881)
                     /locus_tag="OO572_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6663099.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
BASE COUNT          999 a         1371 c         1507 g         1137 t
ORIGIN      
        1 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
       61 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      121 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      181 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      241 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
      301 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      361 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      421 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
      481 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      541 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      601 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      661 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
      721 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
      781 atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat
      841 agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca gtgtgaccgt
      901 gtgcttctca aatgcctgag gccagtttgc tcaggctctc cccgtggagg taataattga
      961 cgatatgatc atttattctg cctcccagag cctgataaaa acggttagcg ccggggttgg
     1021 atttttcagc gttccagcta aggctaaggc attcctgttc aagcgcaagc cgggctataa
     1081 agcgcattat cgctttaccc gtgcccttat ttcgatcgca ctgagaaaca tacagctctt
     1141 taatatgcag ctgaccgctg tatcggggcg agggataaag aatattgcaa catgccaggc
     1201 cagtaatgtt attgccgcag cgcgctctga tcaccagggt accggaaagc cggttgaata
     1261 actttttaca gagataatcc ttcatcaacg cttcctgaat gataccttcg ccataatagg
     1321 ggtcgtctca gaaaacggaa aataaagcac gctaagccgg ttgcagaggc cgtagcggcc
     1381 tgaacttccc cgcgccgatc ttggcgctgc tgcgccatag gtaatcaccg gtcaggttga
     1441 tgtgctccca gccgagtggc gacaggtact gcaatagcga gtcatcgacg gcatgaccat
     1501 tgccgcgcaa cgcatgcgcc gcacgctcca ggtagaccgt gttccacagc acgatggccg
     1561 ccgtcaccag gttgaggccg ctggcccggt agcgctgctg ctcgaaactg cggtcacgga
     1621 tttcaccaag gcggttgaag aacacggcac gggccagcgc attgcgcgcc tcgcccttgt
     1681 tcagcccggc atgcacgcgg cggcgtagct cgacgctttg cagccagtcg aggatgaaca
     1741 gcgtgcgctc gatgcggccc aactcgcgca gcgcgacggc caagccgttc tggcgcgggt
     1801 agctgccgag tttcctgagc atcagcgagg ccgtcaccgt gccctgcttg atcgaggtgg
     1861 ccagccgcag gatttcgtcc caatgggcgc ggacgtgctt gatgttgagc gtgccgccga
     1921 tcatcggctt gagcgcgtca taggcggcat cgcccttcgg gatgtagagc ttggtgtcgc
     1981 ccaggtcgcg gatgcgcggc gcgaagcgga agcccaagag gtgcatcagg gcgaagacgt
     2041 gatcggtgaa gcccgccgtg tcggtgtagt gctcctcgat ccgcaggtcg gattcgtggt
     2101 acagcaggcc gtcgagcacg taggttgagt cgcgcaggcc gacattgacc accttggtgt
     2161 ggaatggcgc gtattggtcg gagatgtggg tgtagaaagt ccgtcctggg ctgctgccat
     2221 attttgggtt gatgtgcccc gtgctctttg ccttgctagc ggttcggaaa ttctgtccgt
     2281 ccgatgatga tgtggtgcca tcgccccagt gcccggcaaa gggatgccga aactgagcgt
     2341 tgaccagttc agccaacgct gtcgagtacg tttcgtcgcg ggtatgccag gcttgcagcc
     2401 aagcgagctt cgcgtaggtc gtgccggggc aggactcggc catcttggtc aggcccaggt
     2461 tgatcgcgtc ggccaggatc gtggtcaaca acaggttctt gtccttggcc agatcgcccg
     2521 atttcaagtg cgtgaagtgc cgggtgaagc ccgtccactc atcgacttcg agcagcagtt
     2581 cggtgatctt gacgtgcggc aggaccatgg ctgtctggtc tatcagcgcc tgcgcggtgt
     2641 cgggcaccgc cgcatccagc ggcgtgatct tcaagcccga ctcggtgatg atggcatccg
     2701 gcaggtcgtt ggctgccgcc atgcggttga cggtggcaag ttgtgcttcc agcagcgtca
     2761 gccgctcatg cagatattgt tcgcagtcgg tggccacggc cagcggcaat tcgctggact
     2821 gcttgaggct ggtgaacttc tcgggcggta ccaggtagtc ctcgaagtcc ttgaactggc
     2881 gtgaaccctg cacccagatg tcgcccgagc gcagggagtt cttcaactcg gacagcgcgc
     2941 acagttcgta gtagcgccgg tcgatgccgg cgtcggtcat caccagtttc tgccagcgcg
     3001 gcttgatgaa gccggtcggt gcatcggctg gcagcttgcg ggcgttgtcg gtgttcatgc
     3061 cgcgcagcac ctcaatggca tcaagcacgt ttttggcggc gggcgcggcc cgcagcttga
     3121 gcacggcaag gaattccggt gcatagcggc gcagggtggc gtagctctcg ccgatgcgat
     3181 gcaggaaatc gaagtcatcg ggttgcgcga gcttctgcgc ctcggtgacg ctctcggcaa
     3241 aggaatccca ggacatgacg gcctcgatgg cggcaaacgc atcgcggcct gattgcttgg
     3301 cgtcgatcag cgcctgaccg atgcgcccgt acagacgtac cttggcgttg atggccttgc
     3361 ctgacgcctg gaactgctgc tgatgcttat tcttggcagc gttaaacagc ttacccagga
     3421 tgcggtcgtg caggtcgatg atttcgtcgg tgacggtggc catgccctcg gtggccagcg
     3481 ccacgagagt ggcgtagcgc cgttgcggct cgaatttggc caggtcggcg ggtgtcatct
     3541 ggccgccctc gcgggcaatc ttgagcaggc ggttctggtg aaccagccgc tcgatgccgg
     3601 taggcagatc gagtgcctgc catgccttga ggcgttcgat gtgttccagc atatgccgcg
     3661 aatttggctt ggccggagac tggcgcaacc aagccaacca ggtcgtcttg ccgttgtccc
     3721 ggcgcttgag cagatcgtcg aggcggcggc gatgcgcgtc cgccagtggt tcggccaagg
     3781 cgtcgtagat gcgccggtta gcacgggtga tcgcctcggc actcgcccgc tcgacggcgt
     3841 tgagggcggg cagaatgacc gactgccgcc gcaggtgccc gatcaaggcg ctggccagca
     3901 cgatgccttt gtcggtttgc atcgccagct cggtcagcat ctggacggcc tgccggtaat
     3961 ggctcatggt gaagggccgg aaaccgaaca cggtttgcag ctcgctcagg tgctcgcgcc
     4021 gggtctgctc ccgctggccg tactcgttcc agctttcgac gccgaccttg agctggtcgg
     4081 cgaccagctt caacaagggc gggaacggta gttcatcgac gcccaggatg acgccgggaa
     4141 agcgcaggta acagagctgc accgcgaagc ccagccgatt ggctggcccg cgccgctgtc
     4201 ggatgatcga gaggtcggta tcgttgaatg tgtaatgtcg gatcaggtcg tccttggagt
     4261 ccggcaacgc cagcaggctt tcccgctcgg cggcggacag gatggaacga cgtggcatat
     4321 ttactgatcc gttctcaagt attgatacag ggtttcgcga ctgattccga attcacgagc
     4381 aagcttggtc ttttgctcgc cagcctcgac acgttggcgc agttcggcaa tacgctcaga
     4441 cgacagggat ttcttcctgc cacggtaagc cccgcgttgc ttggcgagcg caataccctc
     4501 gcgctgacgc tcgcggatca gggcgcgctc gaactcggcg aacgcgccca tcaccgagag
     4561 catcaggttc gccatcggag agtcttcgcc agtaaaactg aggtgttcct tgacgaattc
     4621 gatatgcacg ccgcgttgtg tcagcgtttg cacgatccgg cgcaaatcat cgagattgcg
     4681 cgccaggcga tccatgctat gcaccaccac ggtgtcgccg gtgcgggcga agcttatcag
     4741 cgcttccagt tgcggacgct tgacatcctt gccggatgcc ttgtcgctaa aagcgcgatc
     4801 aaccttgacg ccttccagtt gccgttccgg gttctggtcg aaggtgctga ccctgatata
     4861 cccaatgcgc tgtccagtca tggaattccc tgcaaaatgt cagggaagac tctatgacct
     4921 tcaacgagat atgtcaataa attcaaaatt caatcctatc ctgacgcaat ttacacatgg
     4981 catctgacat caggttaggg tatgcctcaa cctg
//
LOCUS       DAJTYL010000476         3241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.225, whole genome shotgun sequence.
ACCESSION   DAJTYL010000476 DAJTYL010000000
VERSION     DAJTYL010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.225"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..908)
                     /gene="intI2"
                     /locus_tag="OO572_004352"
                     /pseudo
     CDS             complement(1..908)
                     /gene="intI2"
                     /locus_tag="OO572_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1240..1713
                     /locus_tag="OO572_004353"
     CDS             1240..1713
                     /locus_tag="OO572_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6663100.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1808..2332
                     /gene="sat2"
                     /locus_tag="OO572_004354"
     CDS             1808..2332
                     /gene="sat2"
                     /locus_tag="OO572_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6663101.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2390..3181
                     /gene="aadA1"
                     /locus_tag="OO572_004355"
     CDS             2390..3181
                     /gene="aadA1"
                     /locus_tag="OO572_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6663102.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          894 a          719 c          785 g          843 t
ORIGIN      
        1 tgcgtataga tttgcgtggt cttaacatcg ttatgcccta agagttcttg cacagtgcga
       61 atatcacgcc ccgcttgtaa tagatgcgta gcaaacgagt gacgaaatgt atgacaagtg
      121 acacgcttgc taacgatgcc tgctttttgt acggctgcct tcaatgcctt tcgcgcaacg
      181 gagtcatgca gatgatggcg gcataatttg ccgttatacg ggtggttgca gagcgtgctg
      241 gagggaaaga caaacatcca cgccgcttgt cgataagcag aagggtattt gtgatctaaa
      301 gcaaaaggca gcgatggccc tacgccttgt aagttgtcgt cttgctgaat aagccgcgct
      361 tgctcaatga gttattttat tgctgggatt aggcgcgtgg gcagtaggct gtttctgctt
      421 ttcccaccct taccgtcatg cacagtgatg cagccattat caaaatcaaa atctttaacc
      481 cgcaaacgca agcattcatt aatgcgcaaa cctgcaccat acagcagcgt aaaaataact
      541 tggttgcgag tatccataac ctgcaaaatg cgttgcactt catttgcaga gataacagag
      601 ggtagccgtc taggcttgct tgcagggata taatcaatat cgcccaacgg ctgttgtaaa
      661 aacctgttgt acaaaaaagc tagggcattt aaagcgattt tctgcgtgtt tatggctaca
      721 tgtctgctgt ttgctaagct ggataaaaac agcctgacct cttcactgcc catggtctga
      781 ggatgacgtt ttttgtgaaa cagaataaaa cgcttaatcc agtgcaggta agttttttca
      841 gttttcagcg cataaccttt ttgtcgcata tccgtgcgta tagaatttaa aaatggactg
      901 ttagacataa aacgctcctt gtcttgcaac tgtctgccta tacagcctat tctagctggg
      961 atttaaaaaa gtgcctgttt tttacgccta gagatgcttg tttaccggta gagttttaat
     1021 ttaatgctaa ataaattaaa atgttatgag ttctttgggt gagataatgt gcatcgtgca
     1081 agcaggatag acggcatgca cgatttgtaa taacagagtg tcttgtattt ttaaagaaag
     1141 tctatttaat acaagtgatt atattaatta acggtaagca tcagcgggtg acaaaacgag
     1201 catgcttact aataaaatgt taacctctga ggaagaattg tgaaactatc actaatggta
     1261 gctatatcga agaatggagt tatcgggaat ggccctgata ttccatggag tgccaaaggt
     1321 gaacagctcc tgtttaaagc tattacctat aaccaatggc tgttggttgg acgcaagact
     1381 tttgaatcaa tgggagcatt acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt
     1441 acatctgaca atgagaacgt attgatcttt ccatcaatta aagatgcttt aaccaaccta
     1501 aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa aagcctgatc
     1561 gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg tgatgtttac
     1621 tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc ctctaacata
     1681 aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga ttcgcaaacc
     1741 tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc caggcgttag
     1801 gcgtcatatg aagatttcgg tgatccctga gcaggtggcg gaaacattgg atgctgagaa
     1861 ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct ttgaactatc
     1921 taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact ctgatgaaga
     1981 ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg aactcaactc
     2041 aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa
     2101 aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca gacagctcct
     2161 tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg
     2221 tggctttact ctcggcggca ttgacctgtt cacgtataaa actagacctc aagtctcgaa
     2281 cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct aacaattcat
     2341 tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca tgagggaagc
     2401 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2461 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2521 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2581 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2641 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2701 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2761 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2821 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2881 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2941 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3001 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3061 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3121 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3181 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     3241 a
//
LOCUS       DAJTYL010000477         3238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.226, whole genome shotgun sequence.
ACCESSION   DAJTYL010000477 DAJTYL010000000
VERSION     DAJTYL010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.226"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(1..908)
                     /gene="intI2"
                     /locus_tag="OO572_004356"
                     /pseudo
     CDS             complement(1..908)
                     /gene="intI2"
                     /locus_tag="OO572_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1240..1713
                     /locus_tag="OO572_004357"
     CDS             1240..1713
                     /locus_tag="OO572_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6663103.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1808..2332
                     /gene="sat2"
                     /locus_tag="OO572_004358"
     CDS             1808..2332
                     /gene="sat2"
                     /locus_tag="OO572_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6663104.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2390..3178
                     /gene="aadA1"
                     /locus_tag="OO572_004359"
     CDS             2390..3178
                     /gene="aadA1"
                     /locus_tag="OO572_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6663105.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          891 a          719 c          785 g          843 t
ORIGIN      
        1 tgcgtataga tttgcgtggt cttaacatcg ttatgcccta agagttcttg cacagtgcga
       61 atatcacgcc ccgcttgtaa tagatgcgta gcaaacgagt gacgaaatgt atgacaagtg
      121 acacgcttgc taacgatgcc tgctttttgt acggctgcct tcaatgcctt tcgcgcaacg
      181 gagtcatgca gatgatggcg gcataatttg ccgttatacg ggtggttgca gagcgtgctg
      241 gagggaaaga caaacatcca cgccgcttgt cgataagcag aagggtattt gtgatctaaa
      301 gcaaaaggca gcgatggccc tacgccttgt aagttgtcgt cttgctgaat aagccgcgct
      361 tgctcaatga gttattttat tgctgggatt aggcgcgtgg gcagtaggct gtttctgctt
      421 ttcccaccct taccgtcatg cacagtgatg cagccattat caaaatcaaa atctttaacc
      481 cgcaaacgca agcattcatt aatgcgcaaa cctgcaccat acagcagcgt aaaaataact
      541 tggttgcgag tatccataac ctgcaaaatg cgttgcactt catttgcaga gataacagag
      601 ggtagccgtc taggcttgct tgcagggata taatcaatat cgcccaacgg ctgttgtaaa
      661 aacctgttgt acaaaaaagc tagggcattt aaagcgattt tctgcgtgtt tatggctaca
      721 tgtctgctgt ttgctaagct ggataaaaac agcctgacct cttcactgcc catggtctga
      781 ggatgacgtt ttttgtgaaa cagaataaaa cgcttaatcc agtgcaggta agttttttca
      841 gttttcagcg cataaccttt ttgtcgcata tccgtgcgta tagaatttaa aaatggactg
      901 ttagacataa aacgctcctt gtcttgcaac tgtctgccta tacagcctat tctagctggg
      961 atttaaaaaa gtgcctgttt tttacgccta gagatgcttg tttaccggta gagttttaat
     1021 ttaatgctaa ataaattaaa atgttatgag ttctttgggt gagataatgt gcatcgtgca
     1081 agcaggatag acggcatgca cgatttgtaa taacagagtg tcttgtattt ttaaagaaag
     1141 tctatttaat acaagtgatt atattaatta acggtaagca tcagcgggtg acaaaacgag
     1201 catgcttact aataaaatgt taacctctga ggaagaattg tgaaactatc actaatggta
     1261 gctatatcga agaatggagt tatcgggaat ggccctgata ttccatggag tgccaaaggt
     1321 gaacagctcc tgtttaaagc tattacctat aaccaatggc tgttggttgg acgcaagact
     1381 tttgaatcaa tgggagcatt acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt
     1441 acatctgaca atgagaacgt attgatcttt ccatcaatta aagatgcttt aaccaaccta
     1501 aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa aagcctgatc
     1561 gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg tgatgtttac
     1621 tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc ctctaacata
     1681 aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga ttcgcaaacc
     1741 tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc caggcgttag
     1801 gcgtcatatg aagatttcgg tgatccctga gcaggtggcg gaaacattgg atgctgagaa
     1861 ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct ttgaactatc
     1921 taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact ctgatgaaga
     1981 ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg aactcaactc
     2041 aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa
     2101 aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca gacagctcct
     2161 tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg
     2221 tggctttact ctcggcggca ttgacctgtt cacgtataaa actagacctc aagtctcgaa
     2281 cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct aacaattcat
     2341 tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca tgagggaagc
     2401 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2461 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2521 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2581 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2641 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2701 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2761 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2821 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2881 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2941 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3001 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3061 acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc gcgcagatca
     3121 gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     3181 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJTYL010000478         3156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.231, whole genome shotgun sequence.
ACCESSION   DAJTYL010000478 DAJTYL010000000
VERSION     DAJTYL010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.231"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..1280)
                     /gene="pepD"
                     /locus_tag="OO572_004360"
     CDS             complement(<1..1280)
                     /gene="pepD"
                     /locus_tag="OO572_004360"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6663106.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQ"
     gene            1542..2000
                     /gene="gpt"
                     /locus_tag="OO572_004361"
     CDS             1542..2000
                     /gene="gpt"
                     /locus_tag="OO572_004361"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6663107.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2092..>3156
                     /gene="frsA"
                     /locus_tag="OO572_004362"
     CDS             2092..>3156
                     /gene="frsA"
                     /locus_tag="OO572_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6663108.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCP"
BASE COUNT          721 a          817 c          827 g          791 t
ORIGIN      
        1 atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac cagatgcatc
       61 accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc ggttttcgcg
      121 ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc gctgtcgatt
      181 agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac cacaccgacg
      241 ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac accgttcggg
      301 gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc agctttatcg
      361 ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag ctcgtttttc
      421 aggatctcct gataggtatt caccagagat ttcagtgcgt cgactttatc agctgcgaca
      481 gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc gttgaaatcg
      541 ataaggcgca ggtccagttc ttctgcatga cccgccagga agcgcaccag cagtttgttg
      601 gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag accttttaag
      661 gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg caggttggag
      721 gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg
      781 ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg
      841 gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc
      901 agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt ggtgccgcgc
      961 gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg
     1021 gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac cggtttacga
     1081 ttttccatac ctgcggtagc aggttgacga atcaggatat tacctacctg atcgcgttcg
     1141 acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga
     1201 taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat
     1261 aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca
     1321 ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtattga
     1381 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     1441 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     1501 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     1561 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     1621 aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggg gcgttactgg
     1681 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
     1741 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
     1801 ttgatgacct ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg tatccaaaag
     1861 cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg
     1921 ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc
     1981 cgccaatctc cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt gttcttttta
     2041 acttcaggca ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag
     2101 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
     2161 gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc
     2221 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg tattgaccca
     2281 cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg taccgacgat
     2341 gatttatacg ataccgtgat tggctaccgt ggtggcaact ggatttatga gtgggccacc
     2401 caggcgatgg tgtggcaaca aaaagcctgt acggaagaag atccgcaact cagtggtcga
     2461 cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct gaaaggagat
     2521 gacctggccg agcaagcgca ggctttatca aaccgcgcct atgaagaggc cgcccagcgt
     2581 ctgccgggca cgatgcggca gatggagttt accgtacccg gcggtgcgcc catcaccggc
     2641 tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg tggtggtctg
     2701 gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc gcgcggcatt
     2761 gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa gctcacccag
     2821 gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc gtgggtggat
     2881 cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt gcgtctggca
     2941 taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt tcataccctg
     3001 ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt tctggcgagt
     3061 cgtttgggga tgcatgatgc ttccgacgaa gcgttacgcg tggagctgaa tcgctattca
     3121 ttaaaagtgc aaggattgct ggggcgtcgc tgccca
//
LOCUS       DAJTYL010000479         2450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.248, whole genome shotgun sequence.
ACCESSION   DAJTYL010000479 DAJTYL010000000
VERSION     DAJTYL010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.248"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..446)
                     /gene="intI1"
                     /locus_tag="OO572_004363"
     CDS             complement(<1..446)
                     /gene="intI1"
                     /locus_tag="OO572_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6663109.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRIS"
     gene            656..1486
                     /locus_tag="OO572_004364"
     CDS             656..1486
                     /locus_tag="OO572_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6663110.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1599..2390
                     /gene="aadA1"
                     /locus_tag="OO572_004365"
     CDS             1599..2390
                     /gene="aadA1"
                     /locus_tag="OO572_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6663111.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          724 a          579 c          580 g          567 t
ORIGIN      
        1 tcactgatcc gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc
       61 agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac
      121 ggccgaggtc ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag
      181 aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc
      241 gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca
      301 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
      361 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      421 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
      481 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
      541 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttggg cgaacccgga
      601 gcctcattaa ttgttagccg ttaaaattaa gccctttacc aaaccaatac ttattatgaa
      661 aaacacaata catatcaact tcgctatttt tttaataatt gcaaatatta tctacagcag
      721 cgccagtgca tcaacagata tctctactgt tgcatctcca ttatttgaag gaactgaagg
      781 ttgtttttta ctttacgatg catccacaaa cgctgaaatt gctcaattca ataaagcaaa
      841 gtgtgcaacg caaatggcac cagattcaac tttcaagatc gcattatcac ttatggcatt
      901 tgatgcggaa ataatagatc agaaaaccat attcaaatgg gataaaaccc ccaaaggaat
      961 ggagatctgg aacagcaatc atacaccaaa gacgtggatg caattttctg ttgtttgggt
     1021 ttcgcaagaa ataacccaaa aaattggatt aaataaaatc aagaattatc tcaaagattt
     1081 tgattatgga aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc
     1141 atggctcgaa agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat
     1201 tattaatcac aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta
     1261 tctacaagat ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc
     1321 aaatagaacc ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa
     1381 atatgttttt gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa
     1441 agccaagaaa aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca
     1501 aaatcgccca acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg
     1561 ctggcgctcg gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg
     1621 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1681 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     1741 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1801 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1861 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1921 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     1981 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2041 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2101 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2161 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2221 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2281 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     2341 aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa
     2401 ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTYL010000480         2447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.249, whole genome shotgun sequence.
ACCESSION   DAJTYL010000480 DAJTYL010000000
VERSION     DAJTYL010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.249"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            complement(<1..446)
                     /gene="intI1"
                     /locus_tag="OO572_004366"
     CDS             complement(<1..446)
                     /gene="intI1"
                     /locus_tag="OO572_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6663112.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRIS"
     gene            656..1486
                     /locus_tag="OO572_004367"
     CDS             656..1486
                     /locus_tag="OO572_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6663113.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1599..2387
                     /gene="aadA1"
                     /locus_tag="OO572_004368"
     CDS             1599..2387
                     /gene="aadA1"
                     /locus_tag="OO572_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6663114.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          721 a          579 c          580 g          567 t
ORIGIN      
        1 tcactgatcc gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc
       61 agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac
      121 ggccgaggtc ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag
      181 aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc
      241 gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca
      301 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
      361 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      421 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
      481 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
      541 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttggg cgaacccgga
      601 gcctcattaa ttgttagccg ttaaaattaa gccctttacc aaaccaatac ttattatgaa
      661 aaacacaata catatcaact tcgctatttt tttaataatt gcaaatatta tctacagcag
      721 cgccagtgca tcaacagata tctctactgt tgcatctcca ttatttgaag gaactgaagg
      781 ttgtttttta ctttacgatg catccacaaa cgctgaaatt gctcaattca ataaagcaaa
      841 gtgtgcaacg caaatggcac cagattcaac tttcaagatc gcattatcac ttatggcatt
      901 tgatgcggaa ataatagatc agaaaaccat attcaaatgg gataaaaccc ccaaaggaat
      961 ggagatctgg aacagcaatc atacaccaaa gacgtggatg caattttctg ttgtttgggt
     1021 ttcgcaagaa ataacccaaa aaattggatt aaataaaatc aagaattatc tcaaagattt
     1081 tgattatgga aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc
     1141 atggctcgaa agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat
     1201 tattaatcac aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta
     1261 tctacaagat ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc
     1321 aaatagaacc ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa
     1381 atatgttttt gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa
     1441 agccaagaaa aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca
     1501 aaatcgccca acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg
     1561 ctggcgctcg gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg
     1621 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1681 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     1741 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1801 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1861 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1921 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     1981 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2041 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2101 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2161 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2221 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2281 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2341 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     2401 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DAJTYL010000481         1603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.274, whole genome shotgun sequence.
ACCESSION   DAJTYL010000481 DAJTYL010000000
VERSION     DAJTYL010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.274"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            57..1262
                     /gene="tet(B)"
                     /locus_tag="OO572_004369"
     CDS             57..1262
                     /gene="tet(B)"
                     /locus_tag="OO572_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6663115.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1375..>1603)
                     /locus_tag="OO572_004370"
     CDS             complement(1375..>1603)
                     /locus_tag="OO572_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6663116.1"
                     /translation="EKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHI
                     AIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          395 a          298 c          360 g          550 t
ORIGIN      
        1 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
       61 atagttcgac aaagatcgca ttggtaatta cgttactcga tgccatgggg attggcctta
      121 tcatgccagt cttgccaacg ttattacgtg aatttattgc ttcggaagat atcgctaacc
      181 actttggcgt attgcttgca ctttatgcgt taatgcaggt tatctttgct ccttggcttg
      241 gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat
      301 cgctggatta cttattgctg gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt
      361 tgctttcagg gatcacagga gctactgggg ctgtcgcggc atcggtcatt gccgatacca
      421 cctcagcttc tcaacgcgtg aagtggttcg gttggttagg ggcaagtttt gggcttggtt
      481 taatagcggg gcctattatt ggtggttttg caggagagat ttcaccgcat agtccctttt
      541 ttatcgctgc gttgctaaat attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa
      601 ccaaaaatac acgtgataat acagataccg aagtaggggt tgagacgcaa tcgaattcgg
      661 tatacatcac tttatttaaa acgatgccca ttttgttgat tatttatttt tcagcgcaat
      721 tgataggcca aattcccgca acggtgtggg tgctatttac cgaaaatcgc tttggatgga
      781 atagcatgat ggttggcttt tcattagcgg gtcttggtct tttacactca gtattccaag
      841 cctttgtggc aggaagaata gccactaaat ggggcgaaaa aacggcagta ctgctcggat
      901 ttattgcaga tagtagtgca tttgcctttt tagcgtttat atctgaaggt tggttagttt
      961 tccctgtttt aattttattg gctggtggtg ggatcgcttt acctgcatta cagggagtga
     1021 tgtctatcca aacaaagagt catcagcaag gtgctttaca gggattattg gtgagcctta
     1081 ccaatgcaac cggtgttatt ggcccattac tgtttgctgt tatttataat cattcactac
     1141 caatttggga tggctggatt tggattattg gtttagcgtt ttactgtatt attatcctgc
     1201 tatcgatgac cttcatgtta acccctcaag ctcaggggag taaacaggag acaagtgctt
     1261 agttatttcg tcaccaaatg atgttattcc gcgaaatata atgaccctct tgataaccca
     1321 agagggcatt ttttacgata aagaagattt agcttcaaat aaaacctatc tattttattt
     1381 atctttcaag ctcaataaaa agccgcggta aatagcaata aattggcctt ttttatcggc
     1441 aagctctttt aggtttttcg catgtattgc gatatgcata aaccagccat tgagtaagtt
     1501 tttaagcaca tcatcatcat aagctttaag ttggttctct tggatcaatt tgctgacaat
     1561 ggcgtttacc ttaccagtaa tgtattcaag gctaattttt tca
//
LOCUS       DAJTYL010000482         1603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600919
            SAMEA8900455-rid15816063.guided.275, whole genome shotgun sequence.
ACCESSION   DAJTYL010000482 DAJTYL010000000
VERSION     DAJTYL010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900455
            Sequence Read Archive: ERR6004824
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477738.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477738.1
            Genome Coverage       :: 21x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:55:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,372
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 3,973
            CDSs (with protein)               :: 3,973
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 173 of 350
            Pseudo Genes (incomplete)         :: 76 of 350
            Pseudo Genes (internal stop)      :: 136 of 350
            Pseudo Genes (multiple problems)  :: 34 of 350
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900455-rid15816063.guided.275"
                     /serovar="S. flexneri 2b"
                     /isolate="600919"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-16"
     gene            57..1262
                     /gene="tet(B)"
                     /locus_tag="OO572_004371"
     CDS             57..1262
                     /gene="tet(B)"
                     /locus_tag="OO572_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6663117.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1375..>1603)
                     /locus_tag="OO572_004372"
     CDS             complement(1375..>1603)
                     /locus_tag="OO572_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6663118.1"
                     /translation="EKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHI
                     AIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          395 a          297 c          360 g          551 t
ORIGIN      
        1 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
       61 atagttcgac aaagatcgca ttggtaatta cgttactcga tgccatgggg attggcctta
      121 tcatgccagt cttgccaacg ttattacgtg aatttattgc ttcggaagat atcgctaacc
      181 actttggcgt attgcttgca ctttatgcgt taatgcaggt tatctttgct ccttggcttg
      241 gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat
      301 cgctggatta cttattgctg gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt
      361 tgctttcagg gatcacagga gctactgggg ctgtcgcggc atcggtcatt gccgatacca
      421 cctcagcttc tcaacgcgtg aagtggttcg gttggttagg ggcaagtttt gggcttggtt
      481 taatagcggg gcctattatt ggtggttttg caggagagat ttcaccgcat agtccctttt
      541 ttatcgctgc gttgctaaat attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa
      601 ccaaaaatac acgtgataat acagataccg aagtaggggt tgagacgcaa tcgaattcgg
      661 tatacatcac tttatttaaa acgatgccca ttttgttgat tatttatttt tcagcgcaat
      721 tgataggcca aattcccgca acggtgtggg tgctatttac cgaaaatcgt tttggatgga
      781 atagcatgat ggttggcttt tcattagcgg gtcttggtct tttacactca gtattccaag
      841 cctttgtggc aggaagaata gccactaaat ggggcgaaaa aacggcagta ctgctcggat
      901 ttattgcaga tagtagtgca tttgcctttt tagcgtttat atctgaaggt tggttagttt
      961 tccctgtttt aattttattg gctggtggtg ggatcgcttt acctgcatta cagggagtga
     1021 tgtctatcca aacaaagagt catcagcaag gtgctttaca gggattattg gtgagcctta
     1081 ccaatgcaac cggtgttatt ggcccattac tgtttgctgt tatttataat cattcactac
     1141 caatttggga tggctggatt tggattattg gtttagcgtt ttactgtatt attatcctgc
     1201 tatcgatgac cttcatgtta acccctcaag ctcaggggag taaacaggag acaagtgctt
     1261 agttatttcg tcaccaaatg atgttattcc gcgaaatata atgaccctct tgataaccca
     1321 agagggcatt ttttacgata aagaagattt agcttcaaat aaaacctatc tattttattt
     1381 atctttcaag ctcaataaaa agccgcggta aatagcaata aattggcctt ttttatcggc
     1441 aagctctttt aggtttttcg catgtattgc gatatgcata aaccagccat tgagtaagtt
     1501 tttaagcaca tcatcatcat aagctttaag ttggttctct tggatcaatt tgctgacaat
     1561 ggcgtttacc ttaccagtaa tgtattcaag gctaattttt tca
//
